LOCUS       DAJTNP010000001       136760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTNP010000001 DAJTNP010000000
VERSION     DAJTNP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            196..1629
                     /gene="cscA"
                     /locus_tag="OO160_000001"
     CDS             196..1629
                     /gene="cscA"
                     /locus_tag="OO160_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5522295.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1637..2695)
                     /locus_tag="OO160_000002"
     CDS             complement(1637..2695)
                     /locus_tag="OO160_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5522296.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2863..3081
                     /locus_tag="OO160_000003"
                     /pseudo
     CDS             2863..3081
                     /locus_tag="OO160_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3099..4427
                     /gene="dsdA"
                     /locus_tag="OO160_000004"
     CDS             3099..4427
                     /gene="dsdA"
                     /locus_tag="OO160_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5522297.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4535..6073)
                     /gene="emrY"
                     /locus_tag="OO160_000005"
     CDS             complement(4535..6073)
                     /gene="emrY"
                     /locus_tag="OO160_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5522298.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6073..7236)
                     /gene="emrK"
                     /locus_tag="OO160_000006"
     CDS             complement(6073..7236)
                     /gene="emrK"
                     /locus_tag="OO160_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5522299.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7652..8266
                     /gene="evgA"
                     /locus_tag="OO160_000007"
     CDS             7652..8266
                     /gene="evgA"
                     /locus_tag="OO160_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5522300.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8271..11864
                     /gene="evgS"
                     /locus_tag="OO160_000008"
     CDS             8271..11864
                     /gene="evgS"
                     /locus_tag="OO160_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5522301.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11920..13065)
                     /gene="yfdE"
                     /locus_tag="OO160_000009"
     CDS             complement(11920..13065)
                     /gene="yfdE"
                     /locus_tag="OO160_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5522302.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13139..14083)
                     /gene="yfdV"
                     /locus_tag="OO160_000010"
     CDS             complement(13139..14083)
                     /gene="yfdV"
                     /locus_tag="OO160_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5522303.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14153..15847)
                     /gene="oxc"
                     /locus_tag="OO160_000011"
     CDS             complement(14153..15847)
                     /gene="oxc"
                     /locus_tag="OO160_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5522304.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15901..17151)
                     /gene="frc"
                     /locus_tag="OO160_000012"
     CDS             complement(15901..17151)
                     /gene="frc"
                     /locus_tag="OO160_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5522305.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17664..18287)
                     /locus_tag="OO160_000013"
     CDS             complement(17664..18287)
                     /locus_tag="OO160_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5522306.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18584..18859
                     /locus_tag="OO160_000014"
     CDS             18584..18859
                     /locus_tag="OO160_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522307.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18936..19178)
                     /locus_tag="OO160_000015"
     CDS             complement(18936..19178)
                     /locus_tag="OO160_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5522308.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19531..20451
                     /gene="lpxP"
                     /locus_tag="OO160_000016"
     CDS             19531..20451
                     /gene="lpxP"
                     /locus_tag="OO160_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5522309.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20806..20877
                     /gene="ypdK"
                     /locus_tag="OO160_000017"
     CDS             20806..20877
                     /gene="ypdK"
                     /locus_tag="OO160_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5522310.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20942..22180)
                     /gene="alaC"
                     /locus_tag="OO160_000018"
     CDS             complement(20942..22180)
                     /gene="alaC"
                     /locus_tag="OO160_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5522311.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22557..24043
                     /locus_tag="OO160_000019"
                     /pseudo
     CDS             22557..24043
                     /locus_tag="OO160_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24058..24792
                     /gene="ypdB"
                     /locus_tag="OO160_000020"
     CDS             24058..24792
                     /gene="ypdB"
                     /locus_tag="OO160_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5522312.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24805..25662
                     /locus_tag="OO160_000021"
                     /pseudo
     CDS             24805..25662
                     /locus_tag="OO160_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25665..28160)
                     /gene="ptsP"
                     /locus_tag="OO160_000022"
     CDS             complement(25665..28160)
                     /gene="ptsP"
                     /locus_tag="OO160_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5522313.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28185..29222)
                     /gene="ypdE"
                     /locus_tag="OO160_000023"
     CDS             complement(28185..29222)
                     /gene="ypdE"
                     /locus_tag="OO160_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5522314.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29222..30305)
                     /gene="ypdF"
                     /locus_tag="OO160_000024"
                     /pseudo
     CDS             complement(29222..30305)
                     /gene="ypdF"
                     /locus_tag="OO160_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30320..31567)
                     /locus_tag="OO160_000025"
     CDS             complement(30320..31567)
                     /locus_tag="OO160_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5522315.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31589..31915)
                     /locus_tag="OO160_000026"
     CDS             complement(31589..31915)
                     /locus_tag="OO160_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5522316.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32134..33099)
                     /gene="glk"
                     /locus_tag="OO160_000027"
     CDS             complement(32134..33099)
                     /gene="glk"
                     /locus_tag="OO160_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5522317.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33303..34559
                     /locus_tag="OO160_000028"
     CDS             33303..34559
                     /locus_tag="OO160_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5522318.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34674..35000
                     /locus_tag="OO160_000029"
     CDS             34674..35000
                     /locus_tag="OO160_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5522319.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35141..36379)
                     /locus_tag="OO160_000030"
     CDS             complement(35141..36379)
                     /locus_tag="OO160_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5522320.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36715..37917
                     /gene="nupC"
                     /locus_tag="OO160_000031"
     CDS             36715..37917
                     /gene="nupC"
                     /locus_tag="OO160_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5522321.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37967..40079)
                     /locus_tag="OO160_000032"
                     /pseudo
     CDS             complement(37967..40079)
                     /locus_tag="OO160_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40287..40362)
                     /locus_tag="OO160_000033"
     tRNA            complement(40287..40362)
                     /locus_tag="OO160_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40327..40329),aa:Ala,seq:ggc)
     gene            complement(40402..40477)
                     /locus_tag="OO160_000034"
     tRNA            complement(40402..40477)
                     /locus_tag="OO160_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40442..40444),aa:Ala,seq:ggc)
     gene            40698..41057
                     /locus_tag="OO160_000035"
     CDS             40698..41057
                     /locus_tag="OO160_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5522322.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41059..41451
                     /locus_tag="OO160_000036"
     CDS             41059..41451
                     /locus_tag="OO160_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5522323.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41503..42918)
                     /gene="gltX"
                     /locus_tag="OO160_000037"
     CDS             complement(41503..42918)
                     /gene="gltX"
                     /locus_tag="OO160_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5522324.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43177..43252
                     /locus_tag="OO160_000038"
     tRNA            43177..43252
                     /locus_tag="OO160_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43210..43212,aa:Val,seq:tac)
     gene            43297..43372
                     /locus_tag="OO160_000039"
     tRNA            43297..43372
                     /locus_tag="OO160_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43330..43332,aa:Val,seq:tac)
     gene            43377..43452
                     /locus_tag="OO160_000040"
     tRNA            43377..43452
                     /locus_tag="OO160_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43410..43412,aa:Lys,seq:ttt)
     gene            43483..43608
                     /locus_tag="OO160_000041"
     ncRNA           43483..43608
                     /ncRNA_class="other"
                     /locus_tag="OO160_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43728..44069
                     /locus_tag="OO160_000042"
     CDS             43728..44069
                     /locus_tag="OO160_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5522325.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44060..44986)
                     /locus_tag="OO160_000043"
     CDS             complement(44060..44986)
                     /locus_tag="OO160_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5522326.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45076..46074
                     /locus_tag="OO160_000044"
     CDS             45076..46074
                     /locus_tag="OO160_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5522327.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46071..46289)
                     /locus_tag="OO160_000045"
     CDS             complement(46071..46289)
                     /locus_tag="OO160_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5522328.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46291..48306)
                     /gene="ligA"
                     /locus_tag="OO160_000046"
     CDS             complement(46291..48306)
                     /gene="ligA"
                     /locus_tag="OO160_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5522329.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48377..49363)
                     /gene="zipA"
                     /locus_tag="OO160_000047"
     CDS             complement(48377..49363)
                     /gene="zipA"
                     /locus_tag="OO160_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5522330.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49593..50354
                     /gene="cysZ"
                     /locus_tag="OO160_000048"
     CDS             49593..50354
                     /gene="cysZ"
                     /locus_tag="OO160_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5522331.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50539..51510
                     /gene="cysK"
                     /locus_tag="OO160_000049"
     CDS             50539..51510
                     /gene="cysK"
                     /locus_tag="OO160_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5522332.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51894..52151
                     /gene="ptsH"
                     /locus_tag="OO160_000050"
     CDS             51894..52151
                     /gene="ptsH"
                     /locus_tag="OO160_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5522333.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52196..53923
                     /gene="ptsI"
                     /locus_tag="OO160_000051"
     CDS             52196..53923
                     /gene="ptsI"
                     /locus_tag="OO160_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5522334.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53964..54473
                     /gene="crr"
                     /locus_tag="OO160_000052"
     CDS             53964..54473
                     /gene="crr"
                     /locus_tag="OO160_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5522335.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54515..55366)
                     /gene="pdxK"
                     /locus_tag="OO160_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54515..55366)
                     /gene="pdxK"
                     /locus_tag="OO160_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5522336.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55471..55845
                     /locus_tag="OO160_000054"
     CDS             55471..55845
                     /locus_tag="OO160_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5522337.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55878..56601
                     /locus_tag="OO160_000055"
                     /pseudo
     CDS             55878..56601
                     /locus_tag="OO160_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56805..57716)
                     /gene="cysM"
                     /locus_tag="OO160_000056"
     CDS             complement(56805..57716)
                     /gene="cysM"
                     /locus_tag="OO160_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5522338.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57850..58947)
                     /gene="cysA"
                     /locus_tag="OO160_000057"
     CDS             complement(57850..58947)
                     /gene="cysA"
                     /locus_tag="OO160_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5522339.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58937..59812)
                     /gene="cysW"
                     /locus_tag="OO160_000058"
     CDS             complement(58937..59812)
                     /gene="cysW"
                     /locus_tag="OO160_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5522340.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59812..60645)
                     /gene="cysT"
                     /locus_tag="OO160_000059"
     CDS             complement(59812..60645)
                     /gene="cysT"
                     /locus_tag="OO160_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5522341.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60645..61661)
                     /gene="cysP"
                     /locus_tag="OO160_000060"
     CDS             complement(60645..61661)
                     /gene="cysP"
                     /locus_tag="OO160_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5522342.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61965..62756)
                     /gene="ucpA"
                     /locus_tag="OO160_000061"
     CDS             complement(61965..62756)
                     /gene="ucpA"
                     /locus_tag="OO160_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5522343.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62943..63743)
                     /gene="murR"
                     /locus_tag="OO160_000062"
     CDS             complement(62943..63743)
                     /gene="murR"
                     /locus_tag="OO160_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5522344.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63907..64803
                     /gene="murQ"
                     /locus_tag="OO160_000063"
     CDS             63907..64803
                     /gene="murQ"
                     /locus_tag="OO160_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5522345.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64807..66231
                     /gene="murP"
                     /locus_tag="OO160_000064"
     CDS             64807..66231
                     /gene="murP"
                     /locus_tag="OO160_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5522346.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66411..67310)
                     /gene="yfeX"
                     /locus_tag="OO160_000065"
     CDS             complement(66411..67310)
                     /gene="yfeX"
                     /locus_tag="OO160_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5522347.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67406..67981)
                     /locus_tag="OO160_000066"
     CDS             complement(67406..67981)
                     /locus_tag="OO160_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5522348.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68042..68491)
                     /locus_tag="OO160_000067"
     CDS             complement(68042..68491)
                     /locus_tag="OO160_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5522349.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68478..68903)
                     /locus_tag="OO160_000068"
     CDS             complement(68478..68903)
                     /locus_tag="OO160_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5522350.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69117..69986
                     /gene="amiA"
                     /locus_tag="OO160_000069"
     CDS             69117..69986
                     /gene="amiA"
                     /locus_tag="OO160_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5522351.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69990..70889
                     /gene="hemF"
                     /locus_tag="OO160_000070"
     CDS             69990..70889
                     /gene="hemF"
                     /locus_tag="OO160_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5522352.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70895..71947)
                     /gene="eutR"
                     /locus_tag="OO160_000071"
                     /pseudo
     CDS             complement(70895..71947)
                     /gene="eutR"
                     /locus_tag="OO160_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71993..72493)
                     /gene="eutK"
                     /locus_tag="OO160_000072"
     CDS             complement(71993..72493)
                     /gene="eutK"
                     /locus_tag="OO160_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5522353.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72506..73165)
                     /gene="eutL"
                     /locus_tag="OO160_000073"
     CDS             complement(72506..73165)
                     /gene="eutL"
                     /locus_tag="OO160_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5522354.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74347..75138
                     /locus_tag="OO160_000074"
     CDS             74347..75138
                     /locus_tag="OO160_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522355.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75858..76253
                     /locus_tag="OO160_000075"
     CDS             75858..76253
                     /locus_tag="OO160_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5522356.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76271..76534
                     /locus_tag="OO160_000076"
     CDS             76271..76534
                     /locus_tag="OO160_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522357.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76557..77099
                     /locus_tag="OO160_000077"
     CDS             76557..77099
                     /locus_tag="OO160_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522358.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77238..77525
                     /locus_tag="OO160_000078"
     CDS             77238..77525
                     /locus_tag="OO160_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522359.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77564..77770
                     /locus_tag="OO160_000079"
     CDS             77564..77770
                     /locus_tag="OO160_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522360.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78082..78567
                     /locus_tag="OO160_000080"
     CDS             78082..78567
                     /locus_tag="OO160_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5522361.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78548..78826
                     /locus_tag="OO160_000081"
     CDS             78548..78826
                     /locus_tag="OO160_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522362.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78819..80279
                     /locus_tag="OO160_000082"
     CDS             78819..80279
                     /locus_tag="OO160_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5522363.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80313..81122
                     /gene="murI"
                     /locus_tag="OO160_000083"
     CDS             80313..81122
                     /gene="murI"
                     /locus_tag="OO160_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5522364.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81241..81935)
                     /locus_tag="OO160_000084"
     CDS             complement(join(81241..81689,81689..81935))
                     /locus_tag="OO160_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5522365.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82454..83827)
                     /gene="eutA"
                     /locus_tag="OO160_000085"
     CDS             complement(82454..83827)
                     /gene="eutA"
                     /locus_tag="OO160_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5522366.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83802..84281)
                     /gene="eutP"
                     /locus_tag="OO160_000086"
     CDS             complement(83802..84281)
                     /gene="eutP"
                     /locus_tag="OO160_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5522367.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84294..84629)
                     /gene="eutS"
                     /locus_tag="OO160_000087"
     CDS             complement(84294..84629)
                     /gene="eutS"
                     /locus_tag="OO160_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5522368.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84922..87201)
                     /gene="maeB"
                     /locus_tag="OO160_000088"
     CDS             complement(84922..87201)
                     /gene="maeB"
                     /locus_tag="OO160_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5522369.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87490..88440
                     /gene="tal"
                     /locus_tag="OO160_000089"
     CDS             87490..88440
                     /gene="tal"
                     /locus_tag="OO160_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5522370.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88460..90463
                     /gene="tkt"
                     /locus_tag="OO160_000090"
     CDS             88460..90463
                     /gene="tkt"
                     /locus_tag="OO160_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5522371.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90566..91609)
                     /locus_tag="OO160_000091"
     CDS             complement(90566..91609)
                     /locus_tag="OO160_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5522372.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91735..92310)
                     /gene="nudK"
                     /locus_tag="OO160_000092"
     CDS             complement(91735..92310)
                     /gene="nudK"
                     /locus_tag="OO160_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5522373.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92378..94357)
                     /gene="aegA"
                     /locus_tag="OO160_000093"
     CDS             complement(92378..94357)
                     /gene="aegA"
                     /locus_tag="OO160_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5522374.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94563..96280
                     /gene="narQ"
                     /locus_tag="OO160_000094"
                     /pseudo
     CDS             94563..96280
                     /gene="narQ"
                     /locus_tag="OO160_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96482..99595
                     /gene="acrD"
                     /locus_tag="OO160_000095"
     CDS             96482..99595
                     /gene="acrD"
                     /locus_tag="OO160_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5522375.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99694..99753)
                     /gene="ypfM"
                     /locus_tag="OO160_000096"
     CDS             complement(99694..99753)
                     /gene="ypfM"
                     /locus_tag="OO160_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5522376.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100134..100490
                     /locus_tag="OO160_000097"
     CDS             100134..100490
                     /locus_tag="OO160_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5522377.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100494..101621
                     /gene="dapE"
                     /locus_tag="OO160_000098"
     CDS             100494..101621
                     /gene="dapE"
                     /locus_tag="OO160_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5522378.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101649..101849
                     /locus_tag="OO160_000099"
     CDS             101649..101849
                     /locus_tag="OO160_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5522379.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101959..102657)
                     /gene="ypfH"
                     /locus_tag="OO160_000100"
     CDS             complement(101959..102657)
                     /gene="ypfH"
                     /locus_tag="OO160_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5522380.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102731..104746)
                     /gene="tmcA"
                     /locus_tag="OO160_000101"
     CDS             complement(102731..104746)
                     /gene="tmcA"
                     /locus_tag="OO160_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5522381.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104761..105624)
                     /locus_tag="OO160_000102"
     CDS             complement(104761..105624)
                     /locus_tag="OO160_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5522382.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105792..106505)
                     /gene="purC"
                     /locus_tag="OO160_000103"
     CDS             complement(105792..106505)
                     /gene="purC"
                     /locus_tag="OO160_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5522383.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106718..107752)
                     /gene="bamC"
                     /locus_tag="OO160_000104"
     CDS             complement(106718..107752)
                     /gene="bamC"
                     /locus_tag="OO160_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5522384.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107769..108647)
                     /gene="dapA"
                     /locus_tag="OO160_000105"
     CDS             complement(107769..108647)
                     /gene="dapA"
                     /locus_tag="OO160_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5522385.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108793..109365
                     /locus_tag="OO160_000106"
     CDS             108793..109365
                     /locus_tag="OO160_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5522386.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109365..109835
                     /gene="bcp"
                     /locus_tag="OO160_000107"
     CDS             109365..109835
                     /gene="bcp"
                     /locus_tag="OO160_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5522387.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110088..110705
                     /gene="hyfA"
                     /locus_tag="OO160_000108"
     CDS             110088..110705
                     /gene="hyfA"
                     /locus_tag="OO160_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5522388.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110705..112722
                     /gene="hyfB"
                     /locus_tag="OO160_000109"
                     /pseudo
     CDS             110705..112722
                     /gene="hyfB"
                     /locus_tag="OO160_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112733..113680
                     /gene="hyfC"
                     /locus_tag="OO160_000110"
     CDS             112733..113680
                     /gene="hyfC"
                     /locus_tag="OO160_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5522389.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113697..115136
                     /gene="hyfD"
                     /locus_tag="OO160_000111"
     CDS             113697..115136
                     /gene="hyfD"
                     /locus_tag="OO160_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5522390.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115148..115798
                     /gene="hyfE"
                     /locus_tag="OO160_000112"
     CDS             115148..115798
                     /gene="hyfE"
                     /locus_tag="OO160_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5522391.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115803..117389
                     /gene="hyfF"
                     /locus_tag="OO160_000113"
     CDS             115803..117389
                     /gene="hyfF"
                     /locus_tag="OO160_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5522392.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117379..119093
                     /gene="hyfG"
                     /locus_tag="OO160_000114"
                     /pseudo
     CDS             117379..119093
                     /gene="hyfG"
                     /locus_tag="OO160_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119103..119648
                     /gene="hyfH"
                     /locus_tag="OO160_000115"
     CDS             119103..119648
                     /gene="hyfH"
                     /locus_tag="OO160_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5522393.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119645..120403
                     /gene="hyfI"
                     /locus_tag="OO160_000116"
     CDS             119645..120403
                     /gene="hyfI"
                     /locus_tag="OO160_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5522394.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120396..120809
                     /gene="hyfJ"
                     /locus_tag="OO160_000117"
     CDS             120396..120809
                     /gene="hyfJ"
                     /locus_tag="OO160_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5522395.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120839..122668
                     /gene="hyfR"
                     /locus_tag="OO160_000118"
     CDS             120839..122668
                     /gene="hyfR"
                     /locus_tag="OO160_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5522396.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122669..122851
                     /locus_tag="OO160_000119"
     CDS             122669..122851
                     /locus_tag="OO160_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5522397.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122873..123745
                     /locus_tag="OO160_000120"
                     /pseudo
     CDS             122873..123745
                     /locus_tag="OO160_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123783..124844)
                     /locus_tag="OO160_000121"
     CDS             complement(123783..124844)
                     /locus_tag="OO160_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5522398.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125057..126520
                     /gene="bepA"
                     /locus_tag="OO160_000122"
     CDS             125057..126520
                     /gene="bepA"
                     /locus_tag="OO160_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5522399.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126541..126900
                     /gene="arsC"
                     /locus_tag="OO160_000123"
     CDS             126541..126900
                     /gene="arsC"
                     /locus_tag="OO160_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5522400.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127033..127779)
                     /gene="hda"
                     /locus_tag="OO160_000124"
     CDS             complement(127033..127779)
                     /gene="hda"
                     /locus_tag="OO160_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5522401.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127829..129118)
                     /gene="uraA"
                     /locus_tag="OO160_000125"
     CDS             complement(127829..129118)
                     /gene="uraA"
                     /locus_tag="OO160_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5522402.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129204..129830)
                     /gene="upp"
                     /locus_tag="OO160_000126"
     CDS             complement(129204..129830)
                     /gene="upp"
                     /locus_tag="OO160_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5522403.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130154..131191
                     /gene="purM"
                     /locus_tag="OO160_000127"
     CDS             130154..131191
                     /gene="purM"
                     /locus_tag="OO160_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5522404.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131191..131829
                     /gene="purN"
                     /locus_tag="OO160_000128"
     CDS             131191..131829
                     /gene="purN"
                     /locus_tag="OO160_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5522405.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            132001..134067
                     /gene="ppk1"
                     /locus_tag="OO160_000129"
     CDS             132001..134067
                     /gene="ppk1"
                     /locus_tag="OO160_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5522406.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134072..135613
                     /gene="ppx"
                     /locus_tag="OO160_000130"
     CDS             134072..135613
                     /gene="ppx"
                     /locus_tag="OO160_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5522407.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135652..>136760)
                     /gene="pdeF"
                     /locus_tag="OO160_000131"
     CDS             complement(135652..>136760)
                     /gene="pdeF"
                     /locus_tag="OO160_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5522408.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34141 a        35182 c        33606 g        33831 t
ORIGIN      
        1 tgagttacgc atagtgataa acctctttct cgcaaaatcg tcatggattt actaaaacat
       61 gcatattcga tcacaaaacg ccatagttaa cgttaacatt tgcgatattc atcgcattta
      121 tgaaagtaag ggactatatt tttataaaag ttaacgttaa taattcacca aatttgctta
      181 accaggatga ttaaaatgac gcaatctcga ttgcatgcgg cgcaaaacgc actagcaaaa
      241 cttcatgagc accggggtaa cactttctat ccccattttc acctcgcgcc tcctgccgga
      301 tggatgaacg atccaaacgg cctgatctgg tttaacgatc gttatcacgc gttttatcaa
      361 catcacccga tgagcgaaca ctggggacca atgcactggg gacatgccac cagtgacgat
      421 atgatccact ggcagcatga gcctattgcg ctagcgctag gagacgataa tgacaaagac
      481 gggtgttttt caggtagtgc tgtcgatgac aatggtgtcc tctcacttat ctacaccgga
      541 cacgtctggc tcgatggtgc aggtaatgac gatgcaattc gcgaagtaca atgtctggct
      601 accagtcggg atggtattca tttcgagaaa cagggtgtga tcctcactcc accagaagga
      661 atcatgcact tccgcgatcc taaagtgtgg cgtgaagccg acacatggtg gatggtagtc
      721 ggggcgaaag atccaggcaa cacggggcag atcctgcttt atcgcggcag ttcattgcgt
      781 gaatgggcct tcgatcgcgt actggcccac gctgatgcgg gtgaaagcta tatgtgggaa
      841 tgtccggact ttttcagcct tggcgatcag cattatctga tgttttcccc gcagggaatg
      901 aatgccgagg gatacagtta ccgaaatcgc tttcaaagtg gcgtaatacc cggaatgtgg
      961 tcgccaggac gactttttgc acaatccggg cattttactg aacttgataa cgggcatgac
     1021 ttttatgcac cacaaagctt tttagcgaag gatggtcggc gtattgttat cggctggatg
     1081 gatatgtggg aatcgccaat gccctcaaaa cgtgaaggat gggcaggctg catgacgctg
     1141 gcgcgcgagc tatcagagag caatggcaaa cttctacaac gcccggttca cgaagctgag
     1201 tcgttacgcc agcagcatca atctgtctct ccccgcagaa tcagcaataa atatgttttg
     1261 caggaaaacg cgcaagcagt tgagattcag ttgcagtggg cgctgaagaa cagtgatgcc
     1321 gaacattacg gattacagct cggcactgga atgcggctgt atattgataa ccaatctgag
     1381 cgacttattt tgtggcggta ttacccacac gagaatttag acggctaccg tagtattccc
     1441 ctcccgcagc gtgacacgct cgccctaagg atatttatcg atacatcatc cgtggaagta
     1501 tttattaacg acggggaagc ggtgatgagt agtcgaatct atccgcagcc agaagaacgg
     1561 gaactgtcgc tttatgcctc ccacggagtg gctgtgctgc aacatggagc actctggcta
     1621 ctgggttaac ataatatcag gtggaacaac ggatcaacag cgggcaaggg atccgcgtca
     1681 ctcttccccc ttcacgacct tcaataatat gcaatgcagc ttcccgcccg ataatgtcat
     1741 gtggaagctg aattgtggtc agcggcggta aaaacagatg cccgacgcca accagattat
     1801 caaagcccat tacagcgaca tcctgcggga tacgtacccc cttcgccaaa agaacctgat
     1861 aagccacaaa ggctgcgcga tcgttaccac atatcagaac atcaaaatct ggtttgcccg
     1921 atttgaagtg agcattgagt aaacttgcga gatcggtgta gtgatcatca cctgttgcca
     1981 tgtgaaattg tttcacctca gccagatctc gtccagcatc acgccaagcc tgctcaaatc
     2041 cctgccgacg ataccctgtt gccaacgcac tttccggtag ccagaagcat aatggttgac
     2101 gatagcccgc cgcgagcaaa tgctgtgttg attcatattg tgcagtgtaa tcatcaggga
     2161 tataactggg taacgctggg tcatccgcca cacagttcgc caatacaata ttttcaccat
     2221 acagagactc aggcagcgtg atatgtcgca gccccattgt agtatagata atgccatccg
     2281 gacggtgggc aagcagctga cgtgccgcgc gggcagcgtc atcttcagaa aaaatattga
     2341 ttaaaaaact attccagccg aactcgctgg cggtttgctc aatggcaagc agaatatcaa
     2401 cagagaaagg agtggtagcc gtgtcctgcg ccagcacggc gagagtcgac ggcttacgtc
     2461 cttgagcgcg catcttacgg gcggaaagat caggaacata attcagggtc tggattgcct
     2521 gcaatacgcg gtcacgcgtt gcaggacgca cagattctgc attatgcatc acccgggaga
     2581 ctgtcatcat cgacactccc gccaggcgtg cgacatcctt taatgaagcc atacccaagc
     2641 cgtttgccgt aaaacgggca ctgtagcaga aacagacgtc actggcgaga tccaacgccc
     2701 tatcacctga cacagcaata caataaaaaa taacaataat tcccggacaa ttgtccccaa
     2761 ttccgcctct gttctcgcca acgagtaacc atataggcaa gtaagtaaaa catgccaact
     2821 ggcatcgcca acctggagaa gaaaatataa atatggatca ttgtgctgcc actgtatccc
     2881 gacatcagcc cggaaattat tgcgattgct atcggttcag gtgcaattgg ctgcacgatc
     2941 gttacggact cgcttttctg gctggtgaag caatattgcg gcgctacgct caatgaaaca
     3001 tttaaatact atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc
     3061 ctgctgtcat ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact
     3121 cgctcatcgc ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt
     3181 ttaatcctag cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg
     3241 atgttcagga cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttg
     3301 ctgaaacagc tgccactggg gggattattg aatcagaact ggttgccatt ccggctatgc
     3361 aaaaacggct ggaaaaggaa tatcaacaac cgatcagcgg gcaactccta ctgaaaaaag
     3421 atagccattt gcccatttcc ggctctataa aagcacgcgg cgggatttat gaagtcctgg
     3481 cacatgcaga aaaactggct ctggaagcgg ggttgctgac gcttgatgat gactacagca
     3541 aactgctttc tccggagttt aaacagttct ttagccaata cagcattgct gtgggctcaa
     3601 ccggaaatct ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag
     3661 ttcatatgtc tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc catggcgtta
     3721 cggtcgtgga atatgagcaa gattatggtg ttgccgtcga ggaaggacgt aaagcagcgc
     3781 agtctgaccc gaactgtttc tttattgatg acgaaaattc ccgcacgttg ttccttgggt
     3841 attccgttgc tggccagcgt cttaaagcgc aatttgccca gcaaggccgt atcgtcgatg
     3901 ctgataaccc tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg
     3961 cattcgggct taaactggcg tttggcgatc atgttcactg cttttttgcc gaaccaacgc
     4021 actccccttg tatgttgtta ggcgtccata caggattaca cgatcagatt tctgttcagg
     4081 atattggtat cgacaacctt accgcagcgg atggccttgc agttggtcgc gcatcatgct
     4141 ttgtcgggcg ggcaatggag cgtctgctgg atggcttcta tacccttagc gatcaaacca
     4201 tgtatgacat gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa ccttcagcac
     4261 tggcgggtat ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg
     4321 gtttcagcgc agaacaattg cgtaatgcca ctcatctggt gtgggcgacg ggaggtggaa
     4381 tggtgccgga agaagagatg aatcaatatc tggcaaaagg ccgttaataa cgtttcaacg
     4441 caacatcgca atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc
     4501 cggtcttacc cgctataacc ccctcctttt atcatcaccc aacgcctttc gctgtaaacg
     4561 gcggtttcgc aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc gccatacgga
     4621 aaatctcatt tgcagaaata gaaagtgact gctgggttat ttcattatta atttcattaa
     4681 gaactcctga aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaacacgg
     4741 ggttaaattg atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc
     4801 acagcgtcat tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca
     4861 tactcgaggc attggcaaat ttattatctg gcaagcctga aaacgaaatc gttgttaagg
     4921 gtaaaaagaa acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa
     4981 aatcaatcgt tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa
     5041 aactgaatgt cactaacacc cgcatgtcta ttttattgcc ataacgtcct atcaaaggtg
     5101 aaatcaatag tggcatgatg ccgatgggcg cataagcaag cccggcccat atcgcattat
     5161 accccatcgt tttctggagt aactgcggca taaggacgat cgctccagag taaaataaat
     5221 acgcgcaggt gatactcaca ataccaatgg tgaaattacg ggacttaaac aaactgagat
     5281 caagaatcgg gttctctgag gtcgattccc aaatgactaa agagatcaga gaaataactg
     5341 atactactgt taatattatt attgtactcg agttgaacca atccagatcg cgccctttat
     5401 caagcataat ttgcaagcca ccaacaccga gcactaacag ggtcagtctt ggtagattca
     5461 tttttaccgg tgaagtttca gtttctcttc ctttaagtaa ggttaagcat aatgtcagga
     5521 cgataatccc catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa
     5581 tatagccgcc caatatcggc ccacatatcg gagcgataat cacggtcatt gaccataatg
     5641 ccagagcaaa tgttcttttt tctggtggat aattccttaa taataaactc tgtgacagtg
     5701 gaattaacgg ccccgccatt aatccctgaa cgactctaaa aaatatcagc acatcaagat
     5761 tggtcgataa actacacatt aaggaagaca gcgaaaaaaa agtgactgaa agtaaaaata
     5821 accttaattc gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg
     5881 ctacgccaaa cgaggtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag
     5941 atattgtcgg tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta
     6001 atgacaatgc aatagtaacg caccataacg tcccaccggt taatggtgcc ggagttgatt
     6061 tagtgattgc cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat
     6121 cggactggta tcgataacta aagccttact ggtataagca ggcatggagg tcacggttga
     6181 agccagatca ggcatctcag caatgtcttc gtccttcgta tcaatagttg cagtcatcga
     6241 taaaccaatg cgcaaggggt gttccatgag ttcttttgga tcaagagaaa cttcaaccgg
     6301 tacacgctga acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa
     6361 cgcattgccg gttcccatat tgatccctgt cacccgacca tgaaacacaa cattttcacc
     6421 ataaagatcg ctgataatat tgactgattg accaatccgt acatccgtga gttgtgtttc
     6481 tttaaagttg gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc
     6541 ggaactcact gtttcgccga cctgaacact tcgctgggca atatagccag taaccggact
     6601 ccttatatcc gtacgtttaa gcaccaacca ggcttctttg gttgcatccg ccgcttcaac
     6661 tacttgtggc tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc
     6721 ctggatagcg gcattcagtg ccgctttgct acttattaac gtatctttgg tatgctccag
     6781 cgtttctttt gaaataaccc cctgcttcgc taaaggcaca cgacggttat aatcttctaa
     6841 agattgttga tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa
     6901 gtacagttta ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc
     6961 gatagtggca tcagttttat ccagtgaaac taaaatgtca ccctgtcgaa cgtagttcgt
     7021 atctttatga ttaactacag tgacactacc tgagacttgt gcagaaattg gatctgcatt
     7081 ccccgtgaca taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc
     7141 ataggcacct gaaaacgcaa taaataaaac tatcaccaat aaagaaaagt atttttttct
     7201 gttagactgt tttttatttg aattaatctg ttccactatt atctctcatt tcgcatagat
     7261 gaaatttatg aattgaatga aaagagtatt cattcttcat atcaaacaca gtgcatgtgt
     7321 tgcacattat cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag
     7381 aggactaaac cgtggctttt gcaatacaat tcttacgcct gtaggattag tgagaagact
     7441 tatagtgcta acttgaaact ataaatcatc ggtataatcc ctgattttat tgttgacatt
     7501 tcatttatgc cgactattta tatggtatac ttgtcgaatt atcttaaagg aaactcagat
     7561 tttcttattt ttattgagaa aatgagatga cgccttatgt ctgtattact acagggagaa
     7621 gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc
     7681 tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga
     7741 gttaactgaa ggcggaagcg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat
     7801 cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg
     7861 ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt acgggaaaca
     7921 ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga acaatatcat
     7981 tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt
     8041 tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag
     8101 tgtcatgcgg tatattcttg atggcaagga taataatgac attgcggaaa aaatgttcat
     8161 cagcaacaaa actgtcagca cgtataaaag tcgcctgatg gaaaaattag aatgtaaatc
     8221 actgatggat ctttacacat tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt
     8281 taccctattt ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacgaag
     8341 attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat ccagtacgtc
     8401 tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca
     8461 agtcccaaac ggctaccttg ttgcataccg attcgcagca acaggttcgt ggtattaatg
     8521 ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg
     8581 cagatcatca aaaagcaatg gacgcgcttg cagaaggtga agtcgatata gtgttatcac
     8641 atttagttac ttcgccgcct cttaataatg acattgctgc aaccaaacca ttgataatta
     8701 cctttccggc gctggtaacc acccttcacg actcaatgcg accgcttacc tcaccaaaac
     8761 cagtaaatat tgctcgggta gcaaattacc ccccagacga ggtaattcat caatcatttc
     8821 caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc gtctcagctg
     8881 ggcacaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgctatt
     8941 tcactcactc cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat aattttttct
     9001 tgaccagaaa agaatctgtc attcttaatg aagtactcaa tagatttgtt gatgctttaa
     9061 caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac ctggcctttc
     9121 tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcag catcccaatt
     9181 taaaggtgct ggaaaatcct tactcgcccc cctattctat gacggatgaa aatggctcgg
     9241 ttcggggcgt tatgggggac attcttaata ttattacctt gcaaacaggt ttaaattttt
     9301 ctccgatcac cgtttcacac aatatccatg ctggaacaca acttagcccc ggaggatggg
     9361 atataatacc tggcgctatt tatagtgaag atcgagaaaa taatgtttta tttgctgaag
     9421 ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat
     9481 taaaaaaagg aatgaaagtt gccattccat attattatga gctgcattcg caattaaaag
     9541 agatgtatcc ggaggttgaa tggatacagg tcgataatgc cagcgctgca tttcacaaga
     9601 ttaaggaagg tgaacttgat gctctggtcg cgacacagct aaattcgcgt tacatgatcg
     9661 atcattacta tcctaatgaa ctttatcatt ttcttattcc tggcgttccg aatgcatcgc
     9721 tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga
     9781 atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa atgcccaatg
     9841 tgaccattga cacatgggac ctatatagcg agcaatttta tattgttacg acattatccg
     9901 ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc
     9961 gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca ctctcggatt
    10021 ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cataatagtg
    10081 cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca ttagaaaaca
    10141 gtgattcacc ctttaaagat gttttttcta atgcgcatga agtcacagca gaaacgaaag
    10201 aaaatcgaac aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca
    10261 ttaatcactg gcatacatta tgcaatcttc ctgcaagtga caatgcagta tatatttgtg
    10321 gttggcaaga tattactgaa acgcgtgatc taattaatgc actcgaggta gaaaaaaata
    10381 aagcgataaa ggctaccgta gcaaaaagtc agtttctggc aacgatgagt cacgaaataa
    10441 gaacaccaat aagctctatt atgggcttcc tggaacttct gtcgggttct ggtcttagca
    10501 aggagcaacg ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct
    10561 taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac
    10621 aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg
    10681 caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga
    10741 tcgaccctca ggcgtttaag caggtcttat caaatttact gagtaatgct ctcaaattta
    10801 ccaccgaggg ggcagtaaaa attacgacct ccctgggtca cattgatgac aaccacgctg
    10861 ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac
    10921 tgtttaagcg ctacagccaa acaagtgcag gtcgtcagca aacaggttct ggtttaggct
    10981 taatgatctg caaagaatta attaaaaata tgcagggcga tttatcatta gaaagtcatc
    11041 caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag caagtggcga
    11101 ctgtcgaggc aaaagcagaa caacccatca cactacctga aaagttgagc atattaatcg
    11161 cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg
    11221 atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg
    11281 atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac
    11341 tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg
    11401 aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg ttgaccctgg
    11461 atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca cctcagtatc
    11521 gccaccttga tatcgaagcc ctgaaaaata atacggcgaa tgatctacaa ctgatgcagg
    11581 agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg tttcatgcac
    11641 tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta
    11701 acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgctt
    11761 cagatgacag taagcctgaa attcttcagc tgctgaactc tgttaaagaa cacattgcag
    11821 agctggacca ggagattgct gttttctgtc agaaaaatga ctaaattgcg gctcccacaa
    11881 tgttctaatg tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg
    11941 aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca
    12001 gccgctgatt tttatcggat tacccggcat cattattcct ccggcttcaa tcaacatatt
    12061 tctcgcctga gtttgtggca aattaatggc ctcagccaca cttaataacg gcgcgacggg
    12121 cacaccaact tcatgtattt tggctaacca aacttcagct gcctgcgttt ttaacgttcg
    12181 ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatcg
    12241 gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc
    12301 attaccacaa caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat
    12361 gtagggatgg cgatttccca ggcgttgtgg tgacttccct gtcgcaatat atgccatcag
    12421 accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct
    12481 ctggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc
    12541 gcatagatcc gcaagagagg taccaacacg cactggcgga gcatcagggt accctgtttc
    12601 catcattatc ccactcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg
    12661 accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgtaa
    12721 cctttcccat gaaaacccca gtttttccat tgtacctagg cgaaaattct cagctaatac
    12781 atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag
    12841 aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc catccacata
    12901 gggaccaaat gtgcgggtat catcaccatg acctggtggc tcaactttaa ttacccttgc
    12961 gcccatatta taaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat
    13021 aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga
    13081 aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt
    13141 atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaag cccagtacgc
    13201 tcacagccaa tgacgctgta ccagtacggg tgtagacatt aaaccggctg gcaataatga
    13261 ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaaa tgttcgctgt
    13321 tcaaatgaca tgccatacca acgagaagca gcgccagtgg catcagaatc aacttcagga
    13381 aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg
    13441 cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg
    13501 ctgccggaat ttttacccca accagcacca ggatcgttgc cagaacaggt gcccatacta
    13561 ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc
    13621 ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag
    13681 aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg
    13741 caaaccccag gaatccaatg gtaggtgaac ccgcaattaa tgcacataca gctgcttctg
    13801 catgggtacg tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa catccgacaa
    13861 taaccacaag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc
    13921 gggtaataga aacaaataga gccgcaggaa gagcgtagtt taataccagt ttattaaatg
    13981 cgcgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga
    14041 ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa
    14101 gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac
    14161 cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt
    14221 cgataaccac attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac
    14281 gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt
    14341 cataccttgc atggtgcaac agatccgttg gtgatggtgc accagcgcca ctgagatcaa
    14401 caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc ggcaggttat
    14461 atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg
    14521 cgacaaccgg agaaccagaa gtcacgctag caccgatggc atagcccata ccgatgccca
    14581 tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc
    14641 gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg ttctcgcgca
    14701 atacgtcgcg cacagcactt aatgcattaa agtaatttaa tggttgagta tctgtactta
    14761 atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata
    14821 ccagtggagt cgtaaatgtg ttttgtttca gttctgccag cataccttgc atactggatg
    14881 caatatcacc aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa
    14941 tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt gccaataacc
    15001 aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg
    15061 cagctgccgc agaaagtgga tgcgtatctt caaggatccc tttcgccata gacattggca
    15121 ggaatggaat ctgagcactt tcaataaatt cacgaagctg ttcatcagct tgtgaatacg
    15181 ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc
    15241 tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact ttaacaatcg
    15301 tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat
    15361 aaactccgcc agggcgaccc gatacagaga cccggatagc gcgtgccaat gcaatgccaa
    15421 gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt
    15481 ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc
    15541 taatcatgat aatcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga
    15601 ggaatcctgg cgcagaaact gtcaggcaga tccccggttt ttgggtaaga aaaccgcttg
    15661 ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg
    15721 cctgggcatg gcgtgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa
    15781 tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat
    15841 ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc cccgttaata
    15901 tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg
    15961 caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg gcgtaaaggc
    16021 agagaatttc attggacaac caacggtcag atattttcca cgcaacggtt gttccacttc
    16081 aacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca tacttaaaac
    16141 cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt tatcaatagt
    16201 gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gggcatgggc
    16261 tgtactgtat gccggatcgg taatccattc tggtttgccg atggctttac aggtgttttc
    16321 ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt cccagccttt
    16381 acatttcagg atccagccag gctgaccgcc accacctgca ttaccaccgc ggggaactgc
    16441 atcaccaaat gtaccattcg gatactgcgg gtattcttcc agataaccca atttatcgag
    16501 acgctgctgg tcacgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat
    16561 ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat
    16621 cagcaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc
    16681 atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc
    16741 ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg
    16801 tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg
    16861 aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc
    16921 cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag
    16981 cgcatcgata tcaggaatat ctcgcagttg gtgacgcgtt acgtcaccaa cgccgggacg
    17041 ttcaatttta ataacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga
    17101 ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc
    17161 tattagttaa ttaaatatgt gttaatttag cgtttattta aggcataacg ccctctataa
    17221 ataatctgca aaatatcttc gtagcggcac tcgatatttt taaattcctg tatcaagtac
    17281 tgaaaattat tttatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa
    17341 agaagaatat gataaatatt aattcgtggt aatcagagag agtcgcgaca tttgtgatgt
    17401 tattcttaca actgtaagaa gcgccggtga tttacaagta agagtatgat tacgcttttt
    17461 aaagcgtacc ataaagcata acataataaa tgatttgtga tgacacggga ttattgcggt
    17521 ttactttgag gaaaataaaa tgcgtgttta tagaaccgcg agcgccaaat aattttatga
    17581 ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta
    17641 cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctatc gacgatgatt
    17701 ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt
    17761 ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc
    17821 attacgccaa caccggccag gcgaagctct tccattgcgc cctttttatc ccctttcgcc
    17881 attttttcat tggcaatttt tatcgcggct tctttttcgg gtgttgctac ataactctca
    17941 gaaattccga cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta
    18001 cctggcttag cgaactttgc ccattcggta ctgtcatctg acagcaaagc ggaagcttca
    18061 ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc
    18121 acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg
    18181 gtttgctgag tttgctgttg tgccgctacc ggtgcattat cagcagccca tacagttgaa
    18241 gatgccagaa ttgctgtgac catcgtagcc ataattaaac gtttcatact tacctcattc
    18301 tttaacagtt ttagaaaacg ccgccatcat attgggtgag catgaaatga gaaatcagag
    18361 agcaacggtc attttgaaag ggttatctta caattgtagt tattatcgca aagcggcatt
    18421 tagtcttaca gttgtaggaa tatgcctcct gtcgcagaga actattctta tataccacaa
    18481 agagatgtca tttattatat tgatgtaaat attgattaat tatatgtaaa tacagccata
    18541 acatcgtatg gttctcagtg aaccattttc aggataactt tctatgaaag taaacttaat
    18601 acttttcagc ttatttttat tggtctctat tatggcatgc aatgtttttg cattttccat
    18661 ttctggtggt gtaagtgaga ggagctataa agagactgaa aaaacatcag cgatgacgac
    18721 cacacactct acaaaacttc agccatcaca ggcgattttg tttaagatga gagaagatgt
    18781 gccaccatta aacctcacag aagaaatcac gcctacttat ccgacaaagg cgaattatct
    18841 tattcatcct gtgcgatagc gtctgtcaca tggcaggtga acaatcatgt ctgatgctga
    18901 tgtgaacacc tgcccaaatg agccatcctg acccactatc gcctgccata ccccaccata
    18961 aagcgaatca tcaagccaca agccggggca agaaacataa atatccccgt caccagttca
    19021 ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat caccatgccg
    19081 aagaaagaag agaccgccga taccacgttc agtacctgac cgatgtagcc gcttacgcag
    19141 acaatacaca gggcgagaaa catccataaa taaatcatag ctcaacctcc tgccgcgcta
    19201 aaaaacctgt aacttcagtc actttaatag taagacaatc cttaacctct ggcaattttt
    19261 gctaagcatc aatgaaaaca gcacgttaaa tgcaacgcta ttgcgtgcgg aaaatttttt
    19321 actttgcacg attaataatc agtagctgaa agcagtcagc cagtgataaa gtagagccac
    19381 cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt acactcttta caggaaccat
    19441 tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt cgacatggaa tcttctgcga
    19501 tacaacaatt cgtatctaca gaaggtaact atgtttccac aatgcaaatt ttcccgcgag
    19561 tttctacatc ctcgctactg gctcacatgg tttgggcttg gtgtactctg gctttgggta
    19621 cagcttcctt atcctgttct ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca
    19681 ttcctgaaac gtcgtgaatc tatcgcccgt aaaaacctgg aactttgttt cccgcagcat
    19741 tctgcggaag aacgcgagaa gatgattgcc gaaaactttc gttcactcgg catggcgctg
    19801 gtagaaaccg gcatggcatg gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt
    19861 gaagggttgg ataaccttaa acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc
    19921 gtccatttta tgtcgctgga actgggcggc cgcgtgatgg gactgtgcca accaatgatg
    19981 gctacctatc gtccacataa taatcagctg atggaatggg tgcagacccg tgggcgcatg
    20041 cgctctaaca aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg tgcactgaag
    20101 aaaggtgaag cggtatggtt tgctcccgat caggattatg gtcgtaaagg cagctccttc
    20161 gcgccgttct ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt
    20221 ctctctggcg cagccatgtt gaccgtaacg atggtaagaa aagcggatta cagcggatat
    20281 cgtttgttca tcaccccaga gatggaaggc tacccgacag atgaaaatca agccgctgct
    20341 tatatgaaca agattatcga gaaagagatc atgcgcgcac cggagcagta cctctggatc
    20401 caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta acaaactgct
    20461 cttactcttt ccctcctcaa atcggccata ataacctcat gatttttata gcttttttct
    20521 taagtttttt ccttcgctga atatctaatt attaccgtca gaaattaaac tgtcgccgat
    20581 atgagacact tgtaagttac taaaagtaaa taagaaaatg cctcttgtct cgtttcagtt
    20641 ttaggcgtac attagcgccg tctcgagcag tttagctcag aattctgggg tgatgcccac
    20701 ggtaaaatac agggctttta aggtcgcttt gcccggtgtc aactcactgt atcaggtgta
    20761 atgaagtcat tcaggcgtaa cagtaattac gcggagagat gtaaagtgaa atatttcttt
    20821 atgggcattt cttttatggt catcgtttgg gccggtactt ttgccctgat gatctaaaag
    20881 caaactgtgc aatgaaaaac aggagccatc aggctcctgt ttgcatttct gacgacggtg
    20941 tttattccgc gttttcgtga atatgtttgc tgctgacggg tagtaaaccg tcggcacgga
    21001 acatcgcttt aatccctctg atggcctgac gaatacggtc gtggttttca atcagtgcaa
    21061 agcgaacatg cgtatcacca tagtcgccaa agccaatccc cggcgagaca caaaccttcg
    21121 cttcgttaag gagcttcttg gcaaattcca gcgatcccat ggccgcatat ggttccggga
    21181 ttttcgccca gacatacatc gaagccttcg gcatttcgac catccagccc gcttcatgca
    21241 gccctttaac caatacatca cgacggcgtt tgtactgttc ggcaatgtcg cgcacgcact
    21301 gttgatcgcc ctcgagtgcc gcaatcgccg ccacctgcaa cggcgtaaaa gtgccgtaat
    21361 cgtgatagct tttgattcgc gccagggcac tgaccagcgt tttgttgccg accataaagc
    21421 cgatacgcca gcccgccatg ttgtagcttt tcgacagcgt aaagaactcg accgccacat
    21481 cgcgtgcacc cggcacctgc ataattgacg gcgctttcca gccatcgtag acgatatcgg
    21541 cataggccag gtcatggacc accagcacat cgtagcattt cgccagcgcc acaacctttt
    21601 cgaagaactc cagttccacg cattgcgcgg ttgggttcga cgggaagccg aggatcatca
    21661 tcttcggttt cggataactt tcacgaatgg cgcgctccag ttcgttgaag aaatcgacac
    21721 cttccaccag cggcactgag cgtacctgcg ccccggcaat caccgcgcca taaatatgga
    21781 tcgggtaact tggattcggc accagcaccg tgtcaccatg atccagcgtc gccagcatca
    21841 gatgcgccag gccctctttc gaaccaatag tgacgatggc ttctgattcc gggtcgattt
    21901 caacgtcgta gcgatcctga taccagcggg aaatggcgcg acgtaaccgc ggaatgccgc
    21961 gtgaagtgga gtaaccatgc gtgtccgggc gctgggccac agtacataat ttttcgacga
    22021 tatgcggcgg agtcgcaccg tccgggttac ccatgctgaa atcgataata tcttcgccgc
    22081 gccgacgcgc agccattttc agttcagcgg tgatgttaaa aacgtagggc gggagacgat
    22141 caatgcgcgt aaagcgacgt tcagggcgag tgtcagccat aatttcctca gattaacgtt
    22201 agcgcccgga ccgtccgagc gacgctgcca caatggtggc tgttttgaaa atagcctgat
    22261 taatttctgt ctgtccagcg ttttagaaca tttttatcaa tggatttaaa tgaggaactc
    22321 cacttcaaaa acaggaaatt tttctacgct aacaagaaaa tcaccaccaa ttttattaac
    22381 attataattt caataaattt accagccaga agtgcattat caagagatac attgctaata
    22441 caccatttat cttttaatcc ccttttaaat ttatggtttt tcctcaaaaa cggcctgctg
    22501 gtcatcagcc ttcaggttac ctatcataga ggtttaatcc ttattcagag tcacccgtgc
    22561 acgaaatatt caacatgctg ctggcggtct tcgatcgggc agcgttaatg cttatctgcc
    22621 tgttctttct catccgtatc cgcctgtttc gcgaactgtt gcacaagtcg gcgcactccc
    22681 caaaagaatt gctcgccgtt accgccattt tctcgctgtt cgccctgttc agcacctggt
    22741 ccggcgttcc cgtagaaggc tcgctggtga acgtacgtat tatcgcggtg atgtccggcg
    22801 ggattctgtt tggcccgtgg gtaggcatca ttaccggcgt gattgcgggt attcaccggt
    22861 atttaattga tatcggcggc gtgacggcga tcccctgctt tatcaccagc attctggcgg
    22921 gttgtatatc aggctggata aacctgaaaa tccccaaagc acagcgctgg cgctggatat
    22981 cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac gcaaggtctg
    23041 cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata ccgatcatgt
    23101 gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact tcattagccc
    23161 gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg atgaagccca
    23221 ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag gggtcgtgac
    23281 cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat tacaggaaat
    23341 ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg ccgagcagct
    23401 acgtgaaatg gcaaataagg cagagattcg cgccctgcaa agcaaaatta atccccattt
    23461 tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg ataccgctcg
    23521 ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa aagacgatga
    23581 gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca ttgagcaggc
    23641 ccgctttggc gacaagctga cggttatcta tgatattgat gaagaggtga attgctgcat
    23701 tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta ttcagccttg
    23761 caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg tacgcattgc
    23821 ggtgcgagat accgggcacg gcatcgttcc aaaggtgatt gagcgggtcg aagcgaatga
    23881 aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt tattgtatgg
    23941 cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt acattcctaa
    24001 ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag tgatattgtg
    24061 aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg gctaattaaa
    24121 gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt gttgaagttt
    24181 ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc gctggatggc
    24241 gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt gttcatcacc
    24301 gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta cattctcaaa
    24361 ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa
    24421 cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac cattaatctg
    24481 gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac
    24541 gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc
    24601 gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc
    24661 aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat tctgcgactg
    24721 aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt tcgccagtta
    24781 atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt
    24841 ttcgccgatc tgttcagcgg cctagtgctt aacccgcaac tactggggcg cgtctggttt
    24901 gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc ccgtctggat
    24961 atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg aatggtggaa
    25021 ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa caaaccggtg
    25081 atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta cgatagccgc
    25141 accacgtcgt tgttgcatcc tgctcgccag acccagcttc ccagcctgca acgcggtgaa
    25201 ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcagaatatc
    25261 attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa tatgccgccg
    25321 ggtaattcac aaccgcgcgg cgattttctc tatcacagca tttgtaactg ggtttaggat
    25381 aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat cacgcccaat
    25441 catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta tgtgcgttgg
    25501 gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat tcatgaagtg
    25561 gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg gcgtcagttt
    25621 agtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct
    25681 gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag
    25741 tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg
    25801 ttagcaccca gcgtcagcat gatcaccagt tcgacttcgc ccatttctga ctgccagtca
    25861 accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg
    25921 tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg ctgccagaca
    25981 tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt gccgcacagg
    26041 aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt ctccagcgcc
    26101 agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc aatttcctgc
    26161 gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct atccagttga
    26221 cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc atccaggccc
    26281 agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca aatgcctacc
    26341 catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag gaacgatggc
    26401 gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac
    26461 tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa gtggtcgatg
    26521 atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc tgcatgacgc
    26581 aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac ccataagatc
    26641 tgatcgaggc tgtgaaccat cgggatcatc aactgggcgt tgccgaaact ggcggcgcgc
    26701 aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg
    26761 cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg
    26821 ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag tagcacctgc
    26881 tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt
    26941 tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg cgcttccaga
    27001 gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata ggccagctgc
    27061 gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc gacctgataa
    27121 taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcact
    27181 gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag taccgggatc
    27241 gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc cagaatcata
    27301 cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc ttcagccacc
    27361 agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg ccaggtgata
    27421 tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag ataatcgctg
    27481 gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagt
    27541 ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc
    27601 agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctga
    27661 ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg
    27721 gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg
    27781 ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa
    27841 cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc gctgtcgata
    27901 aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta
    27961 atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc cagcgagctt
    28021 ttagcatcag cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct ttgccactgg
    28081 ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag
    28141 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
    28201 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
    28261 tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc
    28321 cccattacca atgtgggtac gccagtaccg gttaagtgca ccgctccacc gtccgtgccg
    28381 ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgccac ggtttcaatc
    28441 cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag catcggcccg
    28501 ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta
    28561 tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc gccgcgtaat
    28621 cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc
    28681 agctcacgta gtaacgtcac cagcagatag caactgaggc gatcatcaaa ggctttgccc
    28741 atcacccgct ggtgagggag gacctgaaaa gtggtatcga aggtgacgcg atcgcctgga
    28801 cgaattcccg cctgcatcac ttcgtcatag gtgcgcgcgc caatgtccac gcgcatggcg
    28861 ctaacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca ctcttcacgg
    28921 gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc
    28981 acgtcaatag cgccttcgcg ggagatgctg cgcaccataa atcccacttc gtccatatgg
    29041 gcgcagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga
    29101 ccatcaaatc gcacttcttt ttgcaggcga gccgcttctt ccagcaggat ctgccgcact
    29161 tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc
    29221 attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt
    29281 gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctagc aaataaatcc
    29341 ccggctccac ggtcagcaac atgcctggct gtagcgtcgt ggtgtcccgc ggtgaaaaac
    29401 gcggatcttc atggacttca atgccgatag cgtgaccagt gttatgaccg aaatagtcgc
    29461 caaatccagc ctcggtaatg acccggcgcg cggcgtcgtc aatctgctgg cagcgcacgc
    29521 cggggcgaat tgcagagatt gccgcgagct gtgcctgcag gacaatctga tagacgtcaa
    29581 acagcgggtg agattcggcg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat
    29641 cagcagtagc cctgatacag cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca
    29701 atcttgtcgc tggctttgcc gtgcggcagc gccccgcgcc agccgctggc gacaatggtg
    29761 tcaaaagagg ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc
    29821 tcgcgctcgc tcatccccgc ctgaataaag cggcgaatat gctccgctcc gcgatcggca
    29881 atcccacagg cgaggcggat tatctccacc tcctctggcg ttttgatttg ccgcagcaca
    29941 tccggcgtgg cgctaaccag tttcgcattg agttcagact tccagcggtg cgcggtttcc
    30001 caactcacct gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttga
    30061 ttgacgatag tggtaagcgt gttcgttgcg tcaagcaaat gcagctggta gccttgcgca
    30121 cgggcttcca cctcaacgaa atagcgcgaa tctaccagaa tgtgcgcact ttcacggcta
    30181 ataaccacat agcctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag
    30241 gagagaagca ccgcatccag ttgttgcacc ttaagccagt tgcgcagcga agcgagtaat
    30301 gtcatctgga ttttccttct tacaggctat cgattaacaa tttgcctttg cggaacatca
    30361 tcagacgcag gaacaccacc atcagcgcag taatgattgc ccccagcgcg atccccgcca
    30421 tataaacgcc caagttagtg accagcggcc atgcccagat agcggattcc gggaaccatt
    30481 gcactgcgcc cagccagaca gctgcggttg agccgacaat cgcgccgacc atatatgacg
    30541 gaatagcggt gatggggctt tccagcgcaa acggaatcgc accctcacta atccccataa
    30601 aggccaggaa catcgcggtc ttaccctgcg gataaagttg agcgttgaac aggcgtttgc
    30661 cggttaaacg acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag
    30721 cgatagaacg agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac
    30781 ctgcggcttt gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg
    30841 cgtacatcag cgcgcccttc tcacctgcgg cagtcagcac agtacggata ccgccgttga
    30901 tccagccacc aaagggagtg atgacgtagt acatcgccag catgacaaaa atggcggaaa
    30961 gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct
    31021 ggttcatcca tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg
    31081 tggacggcac cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt
    31141 gcgtcggctg ggtagacatc aacccgccga taaaaccagc cgggaacgcc agtttgccac
    31201 cgatagagtt cgccacaaat gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc
    31261 cgaaggactg cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc
    31321 cgctattaag agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa
    31381 tcagctggct gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa
    31441 tccctgacat cacatgctgc gggagttcac cccagaagct gcttttagag gcctgcggat
    31501 tcttaactgc cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa
    31561 tggccataac aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga
    31621 tttctttgat gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt
    31681 cgcgtgattc gaaacgttcg ttatcttccg gggtaactgc cacggaatgg atgatgatgg
    31741 tcgcttcggc gatatcctgc gccgtcaggc gattctggat gccgtccgcg ccctgggttt
    31801 caattttcac ttcataaccg gcttctaccg ccgcttcttc cagcacctga gcggccataa
    31861 aagtgtgagc caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta
    31921 attcctcgtt cagtgatagt tgactagtga aattatgaac gtgcaaaaca aatcgccgtt
    31981 accagacaaa taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg
    32041 tcggccttgt gaggggacac aaagaaaaat tgaattaact gtatgattta aaagattatc
    32101 gggagagtta cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg
    32161 taaatgtgca ccggaaccga gaagacccgg atagtcatgg acgatgagat acaccggaat
    32221 atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccggaggc
    32281 tttgaagaac tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt
    32341 cccgagattg agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa
    32401 tgcgcggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt
    32461 gagattttct ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc
    32521 agggccagaa agcacgcgct ccgccgaaac atgaccaatt tccgcacgca atatttcgag
    32581 gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa
    32641 gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc
    32701 gtaaaccgca ataggcttac cttcgaccgg ttctgcgcca ccaaactgaa tcagatgctc
    32761 ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg
    32821 gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat
    32881 tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac
    32941 cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga
    33001 ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt
    33061 ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag
    33121 tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg
    33181 caggctccct gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt
    33241 gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga
    33301 acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg
    33361 ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt
    33421 tgctctggca acgactgccg ggaactctgg ggatagccca ggattcaccc ctctggatca
    33481 tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc
    33541 atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc
    33601 tacctagact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg
    33661 aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac
    33721 cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt
    33781 ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag
    33841 ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta
    33901 ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga cagatggaaa
    33961 tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg
    34021 ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat ccggtgctcg
    34081 tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga ccagtttcgc
    34141 tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg
    34201 attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct
    34261 ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc
    34321 atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg
    34381 tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca
    34441 ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag
    34501 gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat aatgtttagc
    34561 aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc
    34621 gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca
    34681 ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca aatgcaggtg
    34741 aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat tacggtaatt
    34801 actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat gaatggcgca
    34861 aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag cgtaaagcat
    34921 atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg
    34981 gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga tgcgacgcta
    35041 gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac
    35101 gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc agcgccgtcc
    35161 ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag cctgtctgtt
    35221 ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca
    35281 gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc
    35341 gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac
    35401 gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc cccgccagtg
    35461 tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag accgttgccg
    35521 cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag
    35581 tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcaccaga ttgacaaaac
    35641 cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg
    35701 aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg
    35761 tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca
    35821 ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag
    35881 ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac
    35941 gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg
    36001 aaacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg
    36061 ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat
    36121 cacgaatctg ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggatct
    36181 gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc
    36241 tagcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa
    36301 tgaacgcagg tcccattaat gcgagcctca tcttacgcgc cgcccgtccg ctgctactct
    36361 caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta
    36421 tgctaaacga gaatgattac caaattcatt taaatggatt gtggtgattt ctctgataga
    36481 ccaagattat gattacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag
    36541 cacatttaca taactttcag cttccataca caacatagca gaaatgtatg acagatcact
    36601 atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac
    36661 catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac
    36721 cgcgtccttc attttgtact ggcacttgcc gttgttgtga ttctcgcact gctggtaagc
    36781 agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta
    36841 ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa
    36901 atgttcgaaa aactgctcgg atttgccaac gaagggacta acttcgtctt tggtagcatg
    36961 aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct
    37021 gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt
    37081 ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc cgtcagctcc
    37141 ctgattctgg gtcagtcaga aaactttatt gcctataaag atatcctcgg caaaatctcc
    37201 cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt
    37261 ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt actgaacatg
    37321 ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc cagtgaagaa
    37381 aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac
    37441 attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg ctttatcgcc
    37501 ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg ctacagcatc
    37561 tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat gggtgttcct
    37621 tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc
    37681 gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc
    37741 atctctgtgt tcctagtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca
    37801 gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt
    37861 tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac
    37921 catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa tcagctaatg
    37981 gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg
    38041 cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca
    38101 gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata tctttcacaa
    38161 agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat
    38221 atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt
    38281 tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg
    38341 cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca atattctttt
    38401 gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg tcgcaagggt
    38461 gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt
    38521 taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg tcatatttca
    38581 gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga
    38641 gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag
    38701 tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga tgatgtgcgc
    38761 aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc
    38821 catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa
    38881 tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc
    38941 tcagcactaa cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca
    39001 acggcagcag cgtacggcaa attgagcgga tacagtgaac gcgcatcatt aagccgtaat
    39061 gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccgcctctt
    39121 gctccggcgc ttgttccaac gcacgaaagt tgggtaggag cgttaacgga tctgttagcg
    39181 cctgcaaatg ccagcggcga ttaagccatt cactgcgatg ataaatgcgc accatataga
    39241 gcaggcaaac gctaaaggaa atcagcaccg cgagaataaa tgccagcgaa tattcggttt
    39301 ccaccccttg caaaaagttc tgattgtaat tcagaaggca aagcgttgaa acagcccagg
    39361 tgagatttaa aaacggatag cgaagattac cgaccccgag ggtaaagatg atgaagaaaa
    39421 cgggtaccag gtaaccggca ataaagtcgt tttcataagg tgtgcacaac agaagtagca
    39481 gcacgcttaa cgctgccagc cagcttaagg taaatgcgag tgagatagta gaaaagcatg
    39541 ttgtaaatca gcactgcggt gaaaaggctt agtaaatcaa cgaccgtgaa aatggcatcc
    39601 gcatcgccga aaaaggtgga tatctttagc gggaaatcaa agaagctccc cacaagatac
    39661 atgctgcatt tgatgccgat cggcgtcacc aaaccaagcc agaacagacg ttgccagaca
    39721 tatcggctgg tcaatccata acgccaacgc gtccccagct gccagcgtaa tattgcgcaa
    39781 gcacacaaca cgacaaacgt ctggctgccg agcatgaccg cagtctgtaa aaaagagaga
    39841 tgaaagttcc acagattggt gcaaaacatc ccgcataaaa cgggaacgac accgcgccag
    39901 ccaaagataa acaagattga cagcattacg cacagtggca tccacgccag aaagatataa
    39961 ctggaatgga taatggcaag tggcgaaata aaacgggata gctgaataag taccacggtg
    40021 agcgtaaacg ctagtgtgaa tatcttgata ttttttatca ggttatgctc cacaaacatg
    40081 agtacactac tctcactgat cggggttttc aggcacacaa ataataagtt tattcgcatc
    40141 gcgaagcaaa caggggccag cctgttgccg atttttcatc caatgatgct ttcaccaggc
    40201 ggcgctaacc cggacgtaga ctgtattaca aaagcggcaa aaagcagaga caaaaaaccc
    40261 ccgctttgca gcgagggttg gaaatttggt ggagctaagc gggatcgaac cgctgacctc
    40321 ttgcatgcca tgcaagcgct ctcccagctg agctatagcc ccacgatgcg tttacgtacc
    40381 aaatttgctg ggtgcaaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    40441 tgccatgcaa gcgctctccc agctgagcta tagccccgtc acgtaaagct tgtcgagttg
    40501 acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt
    40561 tcaatcgctg aaaaatcagg caaatgaaca attttggaaa accgctcgca ttcagtagtt
    40621 attcatgtca cggtttcctg taaagtggtg ttataaaatg aactactaat agacccacat
    40681 acattcaggg aattgttatg ttcaaggaga ggatgacacc agatgaactt gccagactga
    40741 ccggctatag ccgccagacc attaataaat gggtacgcaa ggaaggctgg acaacgtcac
    40801 caaaacctgg cgtccagggt ggcaaagccc gactggttca cgtcaatgaa caggttcgtg
    40861 aatatattcg caatgctgaa cgtccagaag gtcagggaga agcgcctgcc ctttctggtg
    40921 atgcgccgct tgaagtattg ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa
    40981 aacagtttac atccttgctt ctgcgggaag ggattatcgg attgttacaa cgcttaggga
    41041 ttcgcgatag caaataatat gaaaagatta cgcaataaaa tgaccaccga agaactggct
    41101 gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga ccagagaaaa aggctggaaa
    41161 acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac ttattctggt cgatacgcaa
    41221 gtttgcgagt ttattcagaa cacgccagcc ttccataaca caccaatgtt gatggaagct
    41281 gaagagagaa ttgctgaata cgctcctgga gcacgcgccc cggcgtatcg acaaatcatc
    41341 aacgcgatag ataatatgac agacattgag caggaaaaag tcgcgcaatt tttgtcacgt
    41401 gaaggaattc gcaatttcct cgcccgtctc gatattgacg aatcagcata aataaaaaac
    41461 ggcaggatat tatctcctgc cgtttatctt tttacacgct aattactgct gattttcgcg
    41521 ttcagcaata aaatccagcg ctttgttgat acgctcgata ctgcgggtct taccaattgc
    41581 gtgaacggta acatccagtg ctggagactg ccccgcaccg gttacggcga cacgcagcgg
    41641 cataccaact ttacccatac ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg
    41701 aacgttttca gcggtccagt cagtaatcgc ggccagtttg tcacgaacca cttccagcgg
    41761 ctgacgcgct accggacgca gatgtttttt cgccgcatcg gcatcgaact cagcaaaatc
    41821 ttcgtagaaa taacggcagc tctgtgccat ctctttcagc gtcttgcagc gttcgcccag
    41881 cagtttcacc agatcagcca gctgcgggcc gttacgggta tcgatatttt cctgctcaat
    41941 gtgccactgt aagtgagtag caacatactc cggcggcagc gcgttaatgt agtgatggtt
    42001 cagccacagc agcttgtcgg tgttgaacgc actggcagat ttgctgacgg cattcaaagt
    42061 gaagtatttg atcatctctt cacgagtgaa gatttcctga tcgccgtggg accagcccag
    42121 acgcaccaga tagttcagca gtgcttctgg caaataaccg tcatcacgat actgcattac
    42181 gctgactgcc ccgtgacgtt tggacagttt tttaccgtca tcgccattga tcatagaaac
    42241 gtgcgcgtaa accggcaccg gcgctttcag ggccttaaga atgttgatct ggcgcggcgt
    42301 gttgttgata tggtcttcgc cacggataac gtgggtgatt tccatatccc agtcatcgac
    42361 aaccacacag aagttatagg ttggggaacc atcggtacgg cggataataa gatcgtccag
    42421 ttcctggttg ctgaactcga tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc
    42481 ctgcgggtta gcaaaacgta caacacacgg ttcatcatca gcatgatgct catggctgtg
    42541 gcggcagcga ccgtcataac gcggcttctc acctttcgcc atttgctctt cgcgcagcgc
    42601 ttccaggcgc tctttagagc aatagcattt ataagcagtg ccctcttcca gcatctgatc
    42661 gatcaccgcg ttgtagcgat caaaacgttt ggtctggtag tacggacctt catcccactc
    42721 caggctcagc cagttcatgc catccataat ggcttcgata gcttccggcg tggaacgctc
    42781 aagatcggtg tcttcaatac gcagcacgaa ctcaccgccg tggttacgtg caaaaagcca
    42841 ggagtaaaga gcagtacgcg cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa
    42901 gcgagttttg attttcatga aatggcctta cgtttagaaa gatgccgaca accggcaaat
    42961 cctggaaaaa ttgagtgggc gatattctat cactcccgcc tgattcctca atgtagttcg
    43021 ggtttctacc ttacgcatta ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc
    43081 gatcatcttg cttaaattta cgacgaacga acaatttctt tagaaaatgc gttgactcat
    43141 tttgaactct ccctataatg cgactccaca cagcgggggt gattagctca gctgggagag
    43201 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac caactacttt
    43261 atgtagtctc cgccgtgtag caagaaattg agaagtgggt gattagctca gctgggagag
    43321 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac cacttcgggt
    43381 cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg
    43441 cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctatct gcgatactca
    43501 aagattcgaa cctgcagcag gtttgagttg agcgcagcga aacaacggag ccgctcgcgg
    43561 cgacggcccg aagggcgagc gaagcgagtc atcctgcacg acccaccaat gcaaaaaagc
    43621 gccctaaagg cgcttttttg ctatctgcga tttgtaagat tgatttttct tctcaagttc
    43681 cttgttcttc taccgatatt acctcgtaca gtgacgagga ctgaacaatg gccactatct
    43741 cttcaacttc aattccttcc atacaaacac agagtagtaa cagggcatca caaggtagcg
    43801 atgttgcttc acaaatcgcg cggataagtc agcaaatcat caaactcact caacaaatta
    43861 aagagattgt agacactagc ggcagtgctg aagacaagca aaaacaggcc gaattgattc
    43921 aacagcaaat caccctactg gaaacacaac tggcgcagtt gcagaaacag caggcagaaa
    43981 aggcgcagga gaaagagcag cgtttgtctc tgaatgtatc ccttctaaat cctgttgaaa
    44041 aaacgactca tatcgatatc tatatctgat acaacggatc cccttcccga ccagccatta
    44101 gcgcattgcc ctggtctcgt accacgtccc ataacgcttc agcggcggtg gaaagtgaac
    44161 ggttcttcct gcgcaccagc atcaactggc gttcaaccac cggcgtaatg cgcttaacca
    44221 ccagtgggct accttcaggc aatggtagcg ccagtgcagg aagaatactg atgccgatgc
    44281 ctgcggctac catcgggaac agcgtcgccg gatgtccaat ctcctgcaca atattcgcct
    44341 gaataccatt gcgcgccagc gctgcgtcaa tcagcggtcg gctgcctgac gcgtaatcct
    44401 gcaacaccaa tttcgctccc tgtaatgcct gccagggtac ataatcttcc acggccaaag
    44461 cgctatcgcg atggcaaagc agaaagaaag gctcggaaag aatcgcttca cattgcagat
    44521 cgcccactgg gcctggatcg atgacgatgc caaagtcgac atctccctgg cgaatacttt
    44581 ccatcaccca ctgctgcgga cgatcatgca ggacaaactg aatatctgga tagcggcgat
    44641 ggctttcagc aatgcattgc ggaataagat gcgcggaaat ggtctggctg gcagcgaccc
    44701 gaacttttcc gctcagttgt tgccccatac gcccggtatc gcgcaacgtg ctgttaagtt
    44761 catccagcag tcgctcaaga cgcaaagcca actgctgccc tgcatctgta agcaccactt
    44821 cacgcgtggt tctgtccagc aggcgaacac cagtatgatt ttccagctcc ttcacactgt
    44881 gacttactgc cgactggctc aagccgatac gctctcctgc acgactaaaa cttttctcct
    44941 gcgctactgt gacgaaaacc tttaattgtt ttaaagaata attcatctgt tttcttcatg
    45001 aatggatgca ataaatcaat tttatttctc aaactggaag aagcacaata gaaccatcga
    45061 tcatctggag tctttatgaa actttttcgt atcctcgatc ctttcacctt aaccctgatc
    45121 acggtggtgt tgctggcctc tttctttccg gccagaggcg atttcgtccc cttctttgaa
    45181 aatctgacca ccgcagctat tgccctgctg ttctttatgc acggcgcgaa gttgtcgcgt
    45241 gaggcgatta ttgctggcgg tggtcactgg cgactgcatt tgtgggtaat gtgcagcacc
    45301 ttcgtgctgt ttccgattct gggtgtactg tttgcctggt ggaaaccggt aaatgtcgac
    45361 ccgatgctct actccggttt tctctacttg tgcattctcc cggctaccgt gcagtctgca
    45421 atcgccttca cgtcaatggc gggcggtaac gtcgcggcgg cggtttgttc tgcgtcagca
    45481 tccagcctgc tggggatttt cctttcacca ttgctggttg gtctggtgat gaatgttcac
    45541 ggtgcaaggg gcagccttga gcaggtcggt aaaattatgc tgcaactgct gctgccgttt
    45601 gtgttggggc atctttcccg gccgtggatt ggtgactggg tgtcgcgcaa taaaaaatgg
    45661 attgcgaaaa ctgaccagac gtccattctg ttggtggttt atacagcgtt cagcgaagcc
    45721 gtcgttaatg gtatctggca taaagttggc tggggatcat tgctgtttat cgtggtggtc
    45781 agctgcgttc ttctggctat cgtgattgta gttaacgtct ttatggcacg ccgactgagc
    45841 ttcaataagg cagatgaaat tactatcgtc ttttgtggtt cgaaaaagag tctggcaaat
    45901 ggcatcccga tggcaaacat tctgttcccc acatcggtga tcggtatgat ggtgctgccc
    45961 ctgatgattt tccatcagat ccaattgatg gtctgtgcgg tgctggcgcg tcgatacaaa
    46021 cgccagaccg aacagttaca ggcgcagcag gaaagcagcg ccgataaagc ttaaagcgga
    46081 cgcttcaggg gctggaccag ttgcgtcagc ccctcggttt taatcagcag cgtgatttgc
    46141 attagctcac cgagcttccc tgccgggaac tcatctttgc gggcaaacca cagcagatac
    46201 tcctccggca ggtcaattaa gcgacgccct ttgtatttgc caaacggcat tatcgtattg
    46261 gctatttcaa tcagctgctc tttttccatc tcagctaccc agcaaacgca gcatttccgc
    46321 ttcgtcgatg acttcaatgc ccagttcctg cgccttcgcc agtttagatc ctgcagcttc
    46381 acccgctatc accagatcgg ttttcttcga cacgctgccc gcgactttcg cgcccagttc
    46441 gaccagtcga gctttagcgt catcacgcga catctggctt aagctgcccg taagcaccac
    46501 ggttttacca gcaaacgggc tgtcaatctc ttccgcgttg ataacgatcg gcgcaggcca
    46561 gtgaacacct tccgccaaca gctcgctgat gacattgcgg ttgctttctt cggcaaagaa
    46621 gttgtgaacg tgggatgcaa cgacaatgcc aacatcaggc accttttgca gctcttcaat
    46681 cgaagcggct tccagcgctt ccagcgtgcc gaaatatgcc gccagacctg ctgcggtggc
    46741 ctcgccgact tcacggatgc caagtgcata gaggaagcga gcaaaggtgg tttctttcgc
    46801 tttttccagc gcgttaacca cgttttgtgc caattttggc cccatacgct ccagtccggt
    46861 cagtttgcct gcggtgagtt tgaacagatc tgccggagta tggacatatt ctttttcaac
    46921 cagctgatcg atgattttgt cgcccattcc gtcaacatcc atcgcacggc gggaaacaaa
    46981 gtgtttcagc gactctttac gctgcgcacc gcaaatcagg ccaccggtac agcgggcaac
    47041 cgcttcacct tccacacgct caacgtcaga accacatacc ggacaatgcg tcgggaatac
    47101 aacctcacgg gtatcttccg ggcgttcaga aagcacgacg ttaaccacct gcgggatcac
    47161 gtcgccagcg cggcgaatca ccactttatc gccaatgcgt aaaccaagac gttcgatttc
    47221 atccgcattg tgtaaggttg cgttactcac cagcacgcca gcaacatgga caggttccag
    47281 acgcgcaaca ggcgtaatcg cgccagtacg cccaacctga aactcgacgt cacgcacaaa
    47341 ggtcatctgc tcctgcgccg ggaatttaaa cgcaaccgcc cagcgcgggg cacgcgcgac
    47401 aaagccaagc tgctcctgct gttccagtga gttgacctta atcaccacgc cgtcgatatc
    47461 aaagcccagc gtcgggcggt cttcttccac tttgtgatag aacgccagca cttcttccgc
    47521 cgattcacaa agcgttaccc gatcgctgac cggcaccccc cactttttaa attgcagtaa
    47581 acggccaaga tgagtatccg gcagctcgcc accttccaga acaccaacgc catagcagaa
    47641 aaaagtgagc ggtcgcttcg ctgtaatacg cggatcaagc tgacgcagtg aaccagctgc
    47701 cgcattacgt gggttagcaa aaactttccc gcccgtgcgt cgcgcatctt cgttaatctt
    47761 ttcgaacccc gcctgcggca ggaacacttc accacgcact tccagacgcg ccgggatatt
    47821 ctctccgtgc agcttcagcg gaatggcgcg aatagtacgc acattagacg tgatatcttc
    47881 cccggtggtg ccatcgccac gggtcgcggc actgactaaa acgccatttt catacagaat
    47941 actgacggca aggccatcca gcttcagctc acagcaccag gtgactttct cgttgttttt
    48001 cagacggtcc tgcacacgtt tgttgaaagc aagaaagctt tcttcatcaa aaacgttatc
    48061 cagtgacagc attggtactt catggcgtat ctggctgaaa gctgccagcg gcgcagcacc
    48121 tacacgttgg gtaggcgaat caggcgtaat cagttctgga tgtttggttt ccagctcgcg
    48181 cagttcgcgc atcagcctgt cgtattcagc gtcgggaatt tccggcgcat ccatcacatg
    48241 ataaagatat tcatgatggc aaagcgtcgt tcgcagttct gtcagttgtt gttcgattga
    48301 ttccatatcg caccatcaat gctaaaaacc cccgacaagc gggggttcga agaggagtta
    48361 atttgcctta agtgtatcag gcgttggcgt ctttgacttc gcggatgatg tcctggtact
    48421 cgcgcaattt ctgcggagtc atcatacggc gctgatcgtc aagcacgaca ccgcccactt
    48481 catcggcaat atgctgcgca gattgcagca tcagcttgaa gttctgcagc tcgtcaccgt
    48541 aagacggtac ctgcataaag atagtgacac ccggagtagt gaaatccttc atttcaggat
    48601 caaaggttcc cggtttcacc atattcgcca ggctgaataa cgccgggccg ctgccatccg
    48661 ggctaagatg acgatggtaa atattcatat cgccaaaaat gaagcccgct tgttgaatgc
    48721 tgttaagaag cagttcaccg tttagctcgc taccgtgatg cgccgcgacg ttcatgataa
    48781 tcaccgcttc tttgcgcttc ggtttatcca taactggagc cggttccgct acaggctcag
    48841 gctgtggtgc cgctacgggt tctgcaggct ggaaagcctg ttgtgccggt tgcggtgctg
    48901 aatgcacagg ctgcggcgct ggcgcgacct gtggcgaaac tggctgctgc aacggctgtt
    48961 caggctgcgg ctgataggca ggctgctgca ccggctgcgc tggacgcgga gcatgttgcg
    49021 gcggtacctg cgcttcaggc ggctgctgga ccggttgacg cggctgcgca gacgcataag
    49081 gcggtaggta ctggtgttgc ggcgacggac gagcagcctc atgctcctga gcgttagccg
    49141 gggcatgatt cacgcggtga acacgaacct caccaacgcc ctcatcatct tcgacatcct
    49201 cgtcataaga atcgtcgtca cgttttgact tcattcgttt taatggccga tcgcggaaca
    49261 tagaagatcg ttctttacgg ctggtccaga aaccatgtac cagtaaagcg attatggcga
    49321 tcgcgccaac aatgattaat atcagacgca aatcctgcat cattatattc tctgttgttc
    49381 taacaccttg ccaccacggc aaacatttac tcactaagag tatttgccga ttacctcaag
    49441 tgcaagtgca ctattaactt tcacagcaca aagatagatg aaatcgtgct ttttgctgtt
    49501 ttttcgaaca tatcctaact gtccattgcg caattacccg gtaaaatacg cagaattttc
    49561 ctgggattgg tcaaaaggag ctcatcctga ttatggtttc atcattcaca tctgccccac
    49621 gcagcggttt ttactatttt gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc
    49681 gtttcgttat tttaccgctg ctggtcaata ttttgttgat ggggggcgca ttctggtggc
    49741 tctttacaca gctcgatgtc tggatcccga ctttcatgag ttacgttccg gactggctac
    49801 aatggctgag ttatctgttg tggcctctgg ctgtcatctc tgtgctgtta gtgtttggct
    49861 atttcttctc cacgattgct aactggattg ccgctccgtt taacggttta ttggctgaac
    49921 aactggaagc acgattgact ggcgctacac cgccagatac cgggattttc ggtatcatga
    49981 aagatgtgcc gcgaatcatg aaacgcgaat ggcaaaaatt tgtctggtat ctgccgcgcg
    50041 caattgtatt actaattctt tacttcatcc ctggtattgg gcaaaccgtc gcgccggtac
    50101 tgtggttcct gtttagcgcc tggatgttag ccatccagta ttgcgattac cccttcgata
    50161 accacaaagt gccgtttaaa gagatgcgca ccgccctgcg cacacgcaaa atcaccaata
    50221 tgcagtttgg tgctttaacc agcctgttta cgatgatccc gctgcttaat ctgttcatca
    50281 tgcccgttgc cgtttgtggc gcgacggcga tgtgggtcga ttgctatcgc gataaacacg
    50341 cgatgtggcg gtaacaatct accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg
    50401 cttatgccgc cccttattcc atcttgcatg tcattatttc ccttctgtat atagatatgc
    50461 taaatcctta cttccgcata ttctctgagc gggtatgcta cctgttgtat cccaatttca
    50521 tacagttaag gacaggccat gagtaagatt tttgaagata actcgctgac tatcggtcac
    50581 acgccgctgg ttcgcctgaa tcgcatcggt aacggacgca ttctggcgaa ggtggaatct
    50641 cgtaacccca gcttcagcgt taagtgccgt atcggtgcca acatgatttg ggatgccgaa
    50701 aagcgcggcg tgctgaaacc aggcgttgaa ctggttgaac cgaccagcgg taataccggg
    50761 attgcactgg cctatgtagc tgccgctcgc ggttacaaac tcaccctgac catgccagaa
    50821 accatgagta ttgaacgccg caagctgctg aaagcgttag gtgcaaacct ggtgctgacg
    50881 gaaggtgcta aaggcatgaa aggcgcaatc caaaaagcag aagaaattgt cgccagcaat
    50941 ccagagaaat acctgctgct gcaacaattc agcaatccgg caaaccctga aattcacgaa
    51001 aaaaccaccg gtccggagat ctgggaagat accgacggtc aggttgatgt atttattgct
    51061 ggcgttggga ctggcggtac gctgactggc gtcagccgct acattaaagg caccaaaggc
    51121 aagaccgatc ttatctctgt cgccgttgag ccaaccgatt ctccagttat cgcccaggtg
    51181 ctggcaggtg aagagattaa acctggcccg cataaaattc agggtattgg cgctggtttt
    51241 atcccggcta acctcgatct caagctggtc gataaagtca ttggcatcac caatgaagaa
    51301 gccatttcta ccgcccgtcg tctaatggaa gaagaaggta ttcttgcagg tatctcttct
    51361 ggagcagctg ttgccgcagc gttgaaacta caagaagatg aaagctttac caacaagaat
    51421 attgtggtta ttctaccatc atcgggtgag cgttatttaa gcaccgcatt gtttgccgat
    51481 ctcttcactg agaaagaatt gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc
    51541 acctttttag gtgctttttt gtggcctgct tcaaactttc gcccctcctg gcattgattc
    51601 agcctgtcgg aactggtatt taaccagact aattattttg atgcgcgaaa ttaatcgtta
    51661 caggaaaagc caaagctgaa tcgattttat gatttggttc aattcttcct ttagcggcat
    51721 aatgtttaat gacgtacgaa acgtcagcgg tcaacacccg ccagcaatgg actgtattgc
    51781 gctcttcgtg cgtcgcgtct gttaaaaact ggcgctaaca atacaggcta aagtcgaacc
    51841 gccaggctag actttagttc cacaacacta aacctataag ttggggaaat acaatgttcc
    51901 agcaagaagt taccattacc gctccgaacg gtctgcacac ccgccctgct gcccagtttg
    51961 taaaagaagc taagggcttc acttctgaaa ttactgtgac ttccaacggc aaaagcgcca
    52021 gcgcgaaaag cctgtttaaa ctgcagactc tgggcctgac tcaaggtacc gttgtgacta
    52081 tctccgcaga aggcgaagac gagcagaaag cggttgaaca tctggttaaa ctgatggcgg
    52141 aactcgagta atttcccggg ttcttttaaa aatcagtcac aagtaaggta gggttatgat
    52201 ttcaggcatt ttagcatccc cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga
    52261 cgaaattgtc attgaccgga aaaaaatttc tgccgaccag gttgatcagg aagttgaacg
    52321 ttttctgagc ggtcgtgcca aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg
    52381 tgaaacgttc ggtgaagaaa aagaagccat ctttgaaggg catattatgc tgctcgaaga
    52441 tgaggagctg gagcaggaaa tcatagccct gattaaagat aaacacatga cagctgacgc
    52501 agctgctcat gaagttatcg aaggtcaggc ttctgccctg gaagagctgg atgatgaata
    52561 cctgaaagaa cgtgcggctg acgtacgtga tatcggtaag cgcctgctgc gcaacatcct
    52621 gggcctgaag attatcgacc tgagcgccat tcaggatgaa gtcattctgg ttgccgctga
    52681 cctgacgccg tccgaaaccg cacagctgaa cctgaagaag gtgctgggtt tcatcaccga
    52741 cgcgggtggc cgtacttccc acacctctat catggcgcgt tctctggaac tgcctgctat
    52801 cgtgggtacc ggtagcgtca cctctcaggt gaaaaatgac gactatctga ttctggatgc
    52861 cgtaaataat caggtttacg tcaatccaac caacgaagtt attgataaaa tgcgcgctgt
    52921 tcaggagcaa gtggcttctg aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat
    52981 tacgctggac ggtcaccagg tagaagtatg cgctaacatt ggtacggttc gtgacgttga
    53041 aggtgcagag cgtaacggcg ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat
    53101 ggaccgcgac gcgctgccca ctgaagaaga acagtttgct gcttacaaag cagtggctga
    53161 agcgtgtggc tcgcaggcgg ttatcgttcg taccatggac atcggcggcg acaaagagct
    53221 gccatacatg aacttcccga aagaagagaa cccgttcctc ggctggcgcg ctatccgtat
    53281 cgcgatggat cgtaaagaga tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc
    53341 tttcggtaaa ttgcgcatta tgttcccgat gatcatctct gttgaagaag tgcgtgcact
    53401 gcgcaaagag atcgaaatct acaaacagga actgcgcgac gaaggtaaag cgtttgacga
    53461 gtcaattgaa atcggcgtaa tggtggaaac accggctgcc gcaacaattg cacgtcattt
    53521 agccaaagaa gttgatttct ttagtatcgg caccaatgat ttaacgcagt acactctggc
    53581 agttgaccgt ggtaatgata tgatttcaca cctttaccag ccaatgtcac cgtccgtgct
    53641 gaacttgatc aagcaagtta ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg
    53701 tggtgagctt gctggcgatg aacgtgctac acttctgttg ctggggatgg gtctggacga
    53761 attctctatg agcgccattt ctatcccgcg cattaagaag attatccgta acacgaactt
    53821 cgaagatgcg aaggtgttag cagagcaggc tcttgctcaa ccgacaacgg acgagttaat
    53881 gacgctggtt aacaagttca ttgaagaaaa aacaatctgc taatccacga gatgcggccc
    53941 aatttactgc ttaggagaag atcatgggtt tgttcgataa actgaaatct ctggtttccg
    54001 acgacaaaaa ggataccgga actattgaga tcattgctcc gctctctggc gagatcgtca
    54061 atatcgaaga cgtgccggat gtcgtttttg cggaaaaaat cgttggtgat ggtattgcta
    54121 tcaaaccaac gggtaacaaa atggtcgcgc ctgtagacgg taccattggt aaaatctttg
    54181 aaaccaatca cgcgttctct atcgaatctg atagcggcgt tgaactgttc gtccacttcg
    54241 gtatcgacac cgttgaactg aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc
    54301 gcgtgaaagt tggcgatact gtcattgaat ttgatctgcc gctgctggaa gagaaagcca
    54361 agtctaccct gactccggtt gttatctcca acatggacga aatcaaagaa ctgatcaaac
    54421 tgtccggtag cgtaaccgtg ggtgaaaccc cggttatccg catcaagaag taattcttgc
    54481 cgcagtgaaa aatggcgccc atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa
    54541 tcaattcatc gctctcatgc tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg
    54601 cacggtgcac cgcatcgttt aacgccttcc ctttcagcaa accactgata agctgagcac
    54661 aaaacaggtc gccagtccct ttcaggtcgg tttttacccg tgaatgggaa atgacattca
    54721 cgctgtcggc actgaccacc accacctgca tctcctgatt ttcttcatta ccggaggcgc
    54781 tggtaatcac cacccatttt aatgtgtctg aaagcagact ttttgcggca gcaatggcac
    54841 tgtcgagatc gcggcaattt ttaccggtca ggatttccag ctcaaagata ttgggggtaa
    54901 ttccctgcgc cagcggcagt aaatattgtc gatacgcctc gggaaggtca ggtttgacat
    54961 aaattccgct atcaatatcg ccaatcaccg gatcgaccat gatcaatagg tcaggatggt
    55021 ctttgcgtag cgcagtcagc cactcggcaa ggattttgat ttgcgatgcc gttcccatat
    55081 agcccgtggt tacagcacga agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat
    55141 agccgctaaa ccattcgtcc ggaatcgcac caccgtagaa agtgtcataa tgcggcgtat
    55201 tgctcagcaa taccgtcggc acggcaaaga cattcaagcc gttctgtttt atagcaggca
    55261 ccgcaatgct gttgcctacg cttccgtaca ctacctgcga ctgcacggcg acgatatccg
    55321 cctgcagcgc cctactctta tcgttaaaca acaacaaact actcatttaa ttttttctcc
    55381 ttgccgatga tcctcatcgt attccaaccg aaactttacc tgattctggc agtcaaatcg
    55441 gctattacaa aacaaagata aggtaattca gtgaagaaaa tcatttgtct ggccattacg
    55501 ctgctaatga cactccccgc ttacgcgaag ttaactgttc atgaagaagc ccgcatcaac
    55561 gccatgctgg agggattagc acagaaaaag gatttgatat ttgtgcgcaa cggtgatgaa
    55621 catacctgcg atgaagcggt ttcccacctg cgtctgaagc tcggcaatac ccgtaaccgc
    55681 attgacactg ccgagcagtt tattgataag gtagcttcgt cgtcatcgat taccgggaag
    55741 ccgtatatcg tgaagatccc cggtaagagc gatgagaacg cacagccttt tttacatgcg
    55801 ttaattgcgc agacggataa aacggtgcct gcggaaggaa attaatccgc tttggtaagg
    55861 catttacagg aggtaacatg aaaaaatgct ttatttatca cgatgaaaaa tcgaataaat
    55921 tttggtggat agattacgaa ggggatagtt tagctgtcaa ctatggcaag gtaggtagta
    55981 ttggtaaatt ccatacaaaa gaattcgata atgaagaaca gtgtctgaaa gaagccagta
    56041 aattgattgc cgcaaaaatg aaaaaaggat atcaagaaac tccagacttt aacttcatgg
    56101 atctctacta ttttgatgat gaagagattg ggttacatct taaaacgtca cacccaaact
    56161 tccagtgcca ttttactgat ccactttata tgtgttgctg ggatgaagaa tctccttttg
    56221 gtagcgatga aggtgctgat gctctaaacg aacttgaaaa tagcctccgt tctggactgt
    56281 gctgatttcc ctcaaatgtt aattgaaact atgtggggta tgaaatacat cgctatggac
    56341 agtattcttg aagaggatgt gcgtgcgcaa ttactagccg atgaaatgag ctctatccag
    56401 agcaatatga ttacctacgc gactgcattc ggtcagatca aagtcatggg taaaatctcc
    56461 cataaactta aaaagatggg actcaatgca ctagcgcgtc atcagcttac cgcaaaaatt
    56521 cttcaatggg gtgacggtca ggactcacca atacttcaaa aaatgattga tgaccttact
    56581 gcttttccac acgaaaatta aaaactgcat ttgtcggcag caacaactgc cgacaaatca
    56641 gattattgac aaagtgcact ttgtttatgc cggatgcggc gtaaacgcct gatccggcat
    56701 aaaaatattg ctaattcaat atattgcaga aacattgtag gcctgataag cggagcgcat
    56761 caggcagttt tgcgtttgtc atcagtctcc aatgctatta atccttaaat ccctgccccc
    56821 tgactaaaat gctcttcccc aaacactccg gtggagaggt agcgatcgcc acgatcgcag
    56881 atgatcgcta ccaccaccgc accagggtta gcttttgcca cccgcagtgc tccagcaacc
    56941 gcgccgcctg agctgacgcc acagaatatt ccttcccgca ccgccagttc gcgcatggtg
    57001 ttttccgcat cgcgctgatg aatatccagc acctcatcca ccagcgcagc gttgaaaatc
    57061 cccggcagat attccgcagg ccagcggcga atgcccggaa tactgctgcc ttcttccggt
    57121 tgcaggccaa caatggtcac cggtttggat tgttcgcgca taaagcatga aacgccggtg
    57181 atagtgccgg tcgtccccat gctggagaca aaatgagtga tgcgcccgcc ggtttgctgc
    57241 cagatttccg gcccggtggt ggtgtaatgc gcataagggt tatcgggatt attgaactga
    57301 tcgagcagct ttccttcgcc acgattcgcc atctccagcg ccagatcgcg cgcaccttcc
    57361 atgccctgct ctttggtgac aagaatcagt tccgcaccat aagcacgcat cgccgcacgg
    57421 cgttcctggc tcatgttgtc gggcatcagc aatttcatgc gatagccttt cagcgcggca
    57481 atcattgcca gcgcaatgcc ggtgttacca ctggtggctt cgattaagac atcacccggt
    57541 ttaatttccc cgcgcttttc cgcctcgacg atcatcgaaa gtgccgcacg atctttcacc
    57601 gaacctgccg ggttattgcc ttccagtttt aaccacactt cactgccgtt atccggcccc
    57661 attcgctgca acttcaccag aggcgtattg cctattgttt gttctaatgt actcacaatc
    57721 tctatccata cgtggtgttg cctgatgcga cgcttgcgcg tcttatcagg cctacaggtt
    57781 acaaaccttg ccattaaaaa gcccggatcg cgggaagcgc ctccgggcgt ttaacattca
    57841 ctcaacctat caggcgcttt gtgcgagagc aagttcctca tcgcgggttt caatacgctc
    57901 gtcgccgtta tacagccgcg catgttgcag accaacgaat aaacgctcgc catgctgcgg
    57961 ggcatcgtcg ccatgcatca cgaccgtcag cggttcgttg taccacccca gcggctgcac
    58021 cactaattgg gtgtagtgac ctttcgggct ggcttccagt acctgtaccg gcagcggcga
    58081 atcgagactg gtacggcggc tgatatccac ttcccacggg cgcaggaaga gatccaccgg
    58141 cccctgatac gcaggtgtgt agcccagcgg ccagcgatgc gcgccaacat ggaactgccc
    58201 gccgcgaatg gttccctgca ggcggttcac ttcgcccata aattcgagta caaaacgggt
    58261 cgctggttcg cgccatacct gattcggcgc atcagcctgt tcaatattcc cctgactcat
    58321 caccactaca cgatcggcta cttcggtcgc ctcttcctgg tcgtgggtca caaacacgct
    58381 ggtgaatttc agctcttcat gcagttgacg cagccagcga cgcagctctt tacgcacctg
    58441 cgcatccagc gcgccgaacg gttcatcaag cagcagaatt tgcggttcta cagccagcgc
    58501 gcgcgccagc gccacacgct gtttctggcc gccggaaagc tgcgccggat aacgatccgc
    58561 cagatgcgca agctggacca tttccagcaa ttttgtcact ttcgctttga tggctgcggc
    58621 attcgggcgc tcgcgacgcg gcagcaccgt caggccaaaa gtgatattgt cgaataccgt
    58681 catatggcgg aacagcgcgt aatgctggaa cacgaaaccg actttacgat cgcgcgcgtg
    58741 caggcgactc acgtcggtgc cgtggaagcg aatatgcccg ctggtttgat gctccagccc
    58801 ggcgataatc cgcagcagcg tggttttccc ggaaccggac ggccccagca acgcgaccat
    58861 ctgaccagaa ggaatatcca gtgagatatc gttcagcacc tgggtgcgac caaaagactt
    58921 cttgatattg gcaatctcaa tgctcatgat gctcctcctg ctgcgcgcgt ttttcctgat
    58981 tctccaggcg ccactgcaac atactcttta aaaacagggt gataatcgcc atcaacgtca
    59041 acagcgcggc ggcggtaaag gagccgacgg tgttgtagtc ctgctccaac aattcaatct
    59101 gtaacggcag cgaaagtgtt tcgccgcgaa tcgagccgga aaccaccgac actgcaccaa
    59161 actcgccaat cgcgcgggcg ttggtcagca ccacgccgta aagcagcgcc cagcgaatat
    59221 tcggcaatgt gacgcgacgg aacatctgcc agccggacgc gccaagcaaa atcgccgctt
    59281 cgtcttcctg gctgccctgg cttaacatca ccggcaccag ctcgcgcacc acaaacggac
    59341 acgtcacaaa gatggtgacc agcaccattc cgggccagga gaacataatt tgcagattat
    59401 gttcatccag ccagccgccg agcgggccgt tggagccgta gaacagcaga tacaccagac
    59461 cggcaaccac cggcgataca gcaaacggaa tgtccagtag cgtcagcagt aactggcgtc
    59521 ccgggaagtt aaagcgcgtc accagccagg ccagcagaat gccgaacacc aggtttaccg
    59581 gtacggcaat cagcgcgatc atcaccgtca gccagatggc gtgcagcatg tccggatcgg
    59641 ccagattctg taaaaccggc atcagcccct tgctgaatgc ctgcacgaag atgtaaatca
    59701 tcggcaccag caggatgaac gccgaaacca gcatcccgat gccaatcaga aaccatttgc
    59761 cccagttaat cgggcgcgcg tcataacgct tcaattgggt aacttccgcc attaatgacc
    59821 taccacacgc cgaccaaagc gactttgcag agtgttaatt gagaacagca gcagcagaga
    59881 tgccgcgagg atcaccgaag caatcgcgct cgctgccggg taatcaaact cctgtaagcg
    59941 cacaaaaatc atcagcgacg tcacttccgt cttccacgcg atatttccgg cgataaaaat
    60001 caccgcgcca aattcaccaa gactacgggt aaacgacagc gccacgcccg ccaccagcgc
    60061 cggagaaagc tccggcagca ccactttgca gaaactctgc cagcgcgttg caccaagcgt
    60121 ttccgccgct tcttcatatt ccgggcctaa ctcttccagc accggctgca cggtacgcac
    60181 cacaaacgga atgctggtaa aggccatcgc caccgcaata ccaagccagg tataggtgac
    60241 tttgatatca aacttcgcca gccattcacc gtaaaaaccg tttacggaaa agagcgaggc
    60301 cagcgttaaa ccggcgactg ccgttggcag cgcaaagggt aaatccatca gcgcatcaag
    60361 cagcgtgcgg cccggaaagc gataacgtgt taaaatccac gccatcagca gaccgaaaac
    60421 gccgttaaaa atcgatgcca caaacgccga cagcagcgtc actttgtagg ccgcgaccac
    60481 ctgcgggttg gtgatcacct cccagtactg cgcccagctc atctcagaaa gctgcatcac
    60541 cagcgcagag agcggcagca gcaatatcag gcacacaaac agcagactgg tgccaaggct
    60601 taaagtaaag cccggcagca cgcgtcttga agaaactgca aacattactt acgccccgcc
    60661 gctaacagct tgtctaactc gccgccgctg gtgaagtggg ttttcatcac ttccggccat
    60721 gagccaaatt tgtcttccac gcggaacagt tcggtctgcg ggaatttatc tttcagtttg
    60781 tccatgactt ccgggttatt cacacggtag taatagtcgg tgatgatggt ctgcgcctgt
    60841 gggctgtaga gccagttcag gtaggctttt gcggcttttt ccgtaccgtt ggcctgcaca
    60901 tttttatcga cccacgccac cgggaattcc gccagaatat tggttttcgg gatcaccact
    60961 tcaaagccct gtgcttcata ctgtttacgg atgttgttca cttccgattc aaagctaatc
    61021 agcacatcgc ccaagccacg ctcggcaaaa gtggtggttg cgccacgacc gccggtatcg
    61081 aacacttcaa cgtttttcag gaactgggtc ataaactgtt cggttttggc tttatcgcca
    61141 ccgtcagctt tatccgctgc gccccatgcc gccagatagg tataacgcgc gttacccgac
    61201 gttttcgggt tcgggaaaat cagcttcacg tcggagcgca ccaggtcgtt ccagtcgtgg
    61261 atattcttcg ggttgccctt acgcaccagg aagcccatgg tggagtagaa cggcgagcta
    61321 ttattcggca ggcgcgactg ccagtcagcc gggatcagct tgcctttgtc gtgcaggatt
    61381 tgtacgtcgg tcacctggtt ataagtgaca acgtctgctt ttaagccctg caaaatcgcc
    61441 agcgcctgtt ttgatgaccc ggcatgagat tgttttatcg tcagtttgtc gccgccgtta
    61501 tcttttgccc attgctgttc aaacggcgga ttcagggcgg caaacagctc gcgggagacg
    61561 tcataagaac tgttcagcag ttccgttgcc tgtacatggc ccgccagcag cagagaagcg
    61621 accagcgcga gtgagttctt tttcagtaag ttaacggcca ttgcgcaccc ttataaattt
    61681 aatgactttc taatagccat catatttata acggacgtga aaggagtaac ggttttatat
    61741 accgtttggt gatttggaag ttaaaaaggg aataagaact tggttcaaca ggcgtaattg
    61801 ttgcaggcag tttggacacg gacagcgcgg agaaaccgga gcgtacacat agtacgtgag
    61861 gatttcgagc actgcccggg gccaaaatga caaatgaaat agcctggtga acttagttca
    61921 agacgaaatc ctccccacac atgcagggag gaaacagagg tgaatcagat accgacgcta
    61981 accgtctccg gcagtgtgct gccgccatca atcacattct gtgtaccggt taaatagctg
    62041 gattcatccg atgcgaggaa ggccgccagt tcgccgactt ccagcggatc ggcgaggcga
    62101 cacagcggga ttgcttttgc catttcagtc agcaccgatt ctggatcttc cgggttcgac
    62161 tggcgggcaa tgctttccgc cattggcgtg cggacgtatc ccgggcagat ggcgttaacg
    62221 cgaataccgg attgcgcgta ctccaccgcc agcgatttag tcaggccaac aatcgccgct
    62281 ttcgttaagg catacgccgt ttcgccagga tcggccacca tatcaccagt gactgaagac
    62341 atcatcacaa tgcgaccatc tttgcgcgca atcatctccg gcagcaccgc cttcgtgacg
    62401 ttccatacgc ctttaatatt gatatcaata tggaaatcgc gatcttcatc gctcatatcg
    62461 aggaaactgc ccagacgaca aacgcctgcg ttattcacca ggatatcaat gcgcccttct
    62521 ttttccttcg cgcgtttgat agctgcggct accgacgccg ggtcacgcac atcggcgaca
    62581 accgccgtac agcgatgacc acgaccacac agttcgtccg ctagcttttc gatctcaggg
    62641 gagatatcca gcaagattag gttcgcgcca tgacgtgcaa aagttctggc aattccttcg
    62701 ccaattccct gcaatgcgcc cgtaatcagt gctgtcttgc ccgtgagttt acccatttta
    62761 atgctctcct tgttaataca gagcgttaca ctccattccc tctgaaaata caggataaac
    62821 aaaatattgc tgcggatcag ctcactaatt caggaaaatc tcaatccagt cgctgcttta
    62881 tgcatcattt caggcgttgt gttagctcac tgctgcgctg aatcattttc aatgacgcca
    62941 catcattgat gttgcacaag accaacaaac agtaaatcgg taacagaatt ttgcgccgtg
    63001 cgggtagaca tcgacgagct acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa
    63061 tgcgccagcc gccgcagcgg agagtcagcc agagaagtaa tggcaatcac cgttgccccc
    63121 tgctttcgtg ccgcttctgc gcacaagaca atctcttttt tactgccgct gtaagagata
    63181 gcgatttgta catcgccttt tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtcg
    63241 gcttcgcagg ccacgcgata gccaattttc atcagtttga acgacagatc gcgccccacc
    63301 agcgccgatc cgcccaggcc cgttatctgg ataaacggtg ctttactgat gacctcaatg
    63361 attttttgca gccgcgcgta gtcgaacaac gcacaggttt gctccagcgc cagctctttt
    63421 tcgcgattca gtttgcgggc aatcacttcc agcgaatcat cactggtaat cgaactgtgc
    63481 aggtgcagcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc aattaacgcc
    63541 atacgcaatt cagtaaatcc ttgcgcgccg agcttctggg caaatttcac aatgctcgac
    63601 tggctgatac cgagctgttt cgccatctgc cgtgaagaga cggatttcag ctcgctcact
    63661 ctggcccgca gaaagtcagc aattttttgc tcgttcccgg taaattcact ttccgcattg
    63721 cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa aattccgctt
    63781 aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc tttaagtctg
    63841 tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat agtaaggtca
    63901 ccaccgatgc aactagaaaa gatgattact gaaggctcga acaccgcctc ggctgaaatt
    63961 gaccgcgtat cgacgctgga aatgtgccgg attatcaacg atgaagataa aaccgtaccg
    64021 cctgccgttg atcgcgtcct gccggatatc gccgcggcga tcgatgttat ccacgcccag
    64081 gtcagcggcg gcggtcgtct gatttatctc ggtgcgggaa catccggtcg tctggggatt
    64141 ctggatgcca gcgaatgtcc gcccacctac ggcgtgaaac cgggtctggt ggttggtttg
    64201 attgctggag gcgaatatgc cattcagcac gcggtggaag gagctgaaga tagccgggaa
    64261 ggtggtgtta acgatctgaa aaatattaat ttaacggcac aggatgtggt ggttggcatt
    64321 gctgccagcg gtcgcacgcc gtatgtgatt gccggactgg aatacgcacg ccagctcggc
    64381 tgccgcacag tgggaatttc ctgtaatccg gggagcgccg tttcaaccac cgctgagttt
    64441 gccattacac cgattgtagg tgccgaagtt gttaccggtt cttcgcggat gaaagcaggt
    64501 acagcgcaga aactggtgct caatatgctt tccaccgggc tgatgattaa atccggcaaa
    64561 gtgttcggca acctgatggt cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag
    64621 gtcaatatcg ttaaaaacgc caccggatgt aatgccgaac aagcggaagc ggcgttaatt
    64681 gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct cgatgccgca
    64741 gaagctaaaa aacgcctgga tcaacacggc ggctttattc gtcaggtttt agacaaggaa
    64801 taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc
    64861 ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat
    64921 gacagttcac tggttgatcc caatatcaaa acgctggaag gcgtgaaggg cgtcattttg
    64981 accagcgacc aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg
    65041 atgagcgagc tgctgggcga tgcaccggta caggatgccg cagaaatcgc cgcccagaac
    65101 aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc
    65161 accatcttca cgccgctgat ccccggtttt attgccgccg gtctgctgct ggggatagcg
    65221 acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct acccgacgcg
    65281 ctgaatttta tgaaggtgtt cagcaaaggt ttgttcacct tcctagtgat tctggtgggc
    65341 tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc
    65401 tttttgctcg gttataaccc cgctgcaacc accggttact acgccggttt tcacgatttc
    65461 tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgctgattgc cgcgtgggcc
    65521 tgcgcccgca ttgaaggcat ggtgcgccgc tttatgccgg acgatctcga catgctgctg
    65581 acctcgttaa tcaccctgct gatcaccgct acgcttgcgt acctgatcat tatgccgctg
    65641 ggtggctggt tattcgaagg tatgtcatgg ctgtttatgc acctgaacag taatccgttc
    65701 ggttgtgcgg ttttagccgg gctgttcctg atcgccgtgg tatttggcgt gcatcagggc
    65761 tttattcctg tttacctcgc gttaatggac agccagggat tcaacagctt atttcccatc
    65821 ctttcaatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa
    65881 ccgcacagtg cgctacgcag tcaggtacgc ggggcgatta ttcccggcct gctgggcgtt
    65941 ggtgaaccgc tgatttacgg tgtcaccctg ccccgcatga agccgtttgt taccgcctgt
    66001 ttaggcggcg cggcgggcgg tttgtttatc ggattgatag cctggtgggg tctgccgatg
    66061 ggcttaaaca gcgcctttgg gccgtctggt ctggtggcgc tgccgctgat gacttccgca
    66121 caaggcatcc ttccggcaat ggcggtttat gctggcggga ttctggtggc atgggtttgc
    66181 gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg atattatgaa
    66241 acggacaatg ctctatcttt ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata
    66301 ccctgttctg acagaaggta gttgatcaag tatatgcggc gttaaagcag taataaaaag
    66361 gccagtcagt aatgttactg cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc
    66421 atcaacttgt ccagcgacgg tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc
    66481 aacatcgcat cacgtttgcc atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg
    66541 ttatgcagac gcgcgcagta ggcgcagaag tacagaccgt gagtgccact ggcggtgccg
    66601 tacggcaggc tctggcgaac aatcttcagc cctttgccat cttctttcag atcaacgcgg
    66661 gtgaggtgag aggtttccgg acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg
    66721 cgcccgatca tcatctcctg atcgtgaacg ctcatccggt tgagctgctt caggttgtgc
    66781 tcccaacgct gcacaaacac atagctgccg ccggaatcca cgccgtcttt gataaccgcc
    66841 acttcgcggc gcgtctcttc acccgccggg ttttctgtac cgtcaacaaa gccgctcagg
    66901 tcacgctctt ccacccaacg gaagccgtgg atctcttctt tcacttcaat gcagtcacca
    66961 aaggcttcca tcgccgcctg agcgacagag aagtttacgt cgtgacgcag agaaagaatg
    67021 tggatcaaca catcgaactg ggtcgtcggc gcaaggcctt taccgtagcc cggaaaatct
    67081 ttcagctctt ctgccccaac gccgccgctc agagcgcgcc aggtgttgtt accaaaggca
    67141 accaccgcac caagatgcgc gtccgggaat ttcgcttcaa aagttgccag tttgtcggca
    67201 aatgttttac tggccgcacg cagggcgtca acttcccctt tcacgttggc ttcgatccaa
    67261 atcgccgcgc ggcaatgttc tggcaaaatg ccactctgaa cctgagacat tgttcctcct
    67321 gaaaataaga atgccacgca atccgtggca ttgttgagcg ctattttacg tgttttttcg
    67381 cgacggcggt ttgttcagac gcaaattaac gccgccagat aattttactg actttccagt
    67441 ttttcagggt atcgtccgaa ggcattaacc cttcaggacc acgccattcc ccgctgaact
    67501 ggtagctaat atgttgactg ccttcggctt tacattcgac ggcacgatta tcatcaccat
    67561 cagctttttg gcaattgcca aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt
    67621 taacaccggt gtcagcagga atatcgctat ccagcacgtc aatgcgactg atcgtgcctt
    67681 gatcgccgtt aatcaccatc gccacgttgt cgcctttcat cacttcaaaa aagcgcacca
    67741 cgttgccgtt cgtggttttc attccgctgc gcaggcgata atcgccatca agcgcatcag
    67801 caatggcttg ttcttgcagt ggtgtggacg ccgttaattc acccacgccc tgctcgctca
    67861 ctttggtgga cgatccaaac cagttccacg ggttagcggc agaccagtta actgacgaca
    67921 tcgtggaaca gccggtcagc atcaacggca tagcgcataa cattaaacgc agcgatttca
    67981 tgtcacttcc tttggttatt caataacgtt gcttggagtg caaattcacc aaaaagtgcc
    68041 gttattcttt ttcttcatta aaacaagcgc gtaaacgtcg attggtcagc agccagatta
    68101 acgccacaat atccgccact accagcgcca gaccgatacc gctaacggat tcaccgttca
    68161 gccacagcca cggttgccag caaagtaaaa ccacctgcgc caacagcaac agaaaataga
    68221 gcacatgcca ggtgcgagga aacgaagccc gccgaccgct caacaaaaac gccagcaccg
    68281 ccggaatacc gggaatcagc cccagccaga aattatcgtg atcgggataa aacagattta
    68341 gcagcgcagt accctgctca cgcgacgcac cggcaatgac aaacagcacc caggttcgcg
    68401 cctgaagcaa tagcacaagc cagaagagca agggtaaacg caggcgtccg tgcgcatcat
    68461 aatggacagg atggaactca gtactcttca tcttcaatca aacgcttacc cagactcagc
    68521 acgtcggcgt gttcatatcc caggcgttca tacattccga gcaccatgtc gttatcttcc
    68581 ggcacgttga tctgaatttt cgggcagccg cgagcaatca gctttttctc cagccggtta
    68641 agcaacgcat tggcaattcc acgcccacga aactctggat gcacgccaag ataataagca
    68701 gacccgcgat gcccgtcata accgcccatc accgttccga ccacttcacc gtttacctca
    68761 gcgaccaaaa acaaactgac gtcatggttc atcttacgct cgatgtccat ttccggatcg
    68821 ttccacggac gcagcaagtc gcaacgctcc caaagggtga tgacctcttc gaaatcttcc
    68881 tggcgaaata cgcgtatctc catggtattc gttacctttt tgcgggttaa aaggctgatt
    68941 atggcgtgaa cggtcgaatt agccaatatc tgacgaaaat cggttgaaaa agtggcataa
    69001 tggggagttg tcaactattg aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta
    69061 acggattacg cgatacgata taacatctgg aactttatta ttacaactca ggccgtatga
    69121 gcacttttaa accactaaaa acactcactt cgcgccgcca ggtgctgaaa gccggtttag
    69181 ctgccctgac gttgtcagga atgtcgcaag ccattgccaa agacgaacct ttaaaaacca
    69241 gcaacggaca cagcaagccg aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg
    69301 atccaggcca cggcggaatt gataccggag cgatcggacg caacggttcg aaagaaaaac
    69361 atgtggtgct ggcgattgct aaaaacgtcc gttccatttt gcgtaatcat gggattgatg
    69421 cgcgtttaac gcgttctggc gatacgttta tcccacttta cgatcgcgtt gaaatcgccc
    69481 ataaacatgg cgcagatctg tttatgtcaa ttcatgccga tggctttacc aacccgaaag
    69541 ctgccggtgc ttcggtattt gccctctcta accgtggggc aagtagcgca atggcgaaat
    69601 acctgtctga acgcgaaaac cgcgccgatg aagttgccgg taaaaaggcg actgacaagg
    69661 atcacctatt gcagcaagta ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc
    69721 tgacgcttgg ctcgcatatt ctgaaaaaga ttaagccggt gcataaactg cacagccgca
    69781 acaccgaaca agccgcgttt gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg
    69841 aaacctcgtt tatcaccaac ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc
    69901 agaaaatcgc cacagcgatt gctgaaggcg tgatcagtta tttccactgg ttcgacaacc
    69961 agaaagcaca ttcgaaaaag cgataagtta tgaaacccga cgcacaccag gttaaacagt
    70021 ttctgctcaa ccttcaggat acgatttgtc agcagctgac cgccgtcgat ggcgcagaat
    70081 ttgtcgaaga tagttggcag cgcgaagctg gcggcggcgg gcgcagtcgg gtgttgcgta
    70141 atggtggtgt tttcgaacag gcgggcgtca acttttctca cgtccacggt gaggcgatgc
    70201 ctgcttccgc caccgctcat cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg
    70261 tttcactggt agtgcatccg cataacccgt atgttcccac cagccacgcg aatgtgcgtt
    70321 tttttattgc cgaaaaaccg ggtgcagagc ccgtttggtg gtttggcggc ggcttcgatt
    70381 taaccccttt ctatggtttt gaagaagacg ccattcactg gcaccgcacc gcccgtgacc
    70441 tgtgccagcc atttggtgaa gacgtttatc cccgttacaa aaagtggtgc gacgattact
    70501 tctacctcaa acatcgcaac gaacagcgcg gcattggcgg gctgttcttt gatgatctga
    70561 acacgccaga tttcgaccac tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg
    70621 acgcttattt accaattgta gagcgacgta aagcgatggc ctacggcgag cgcgagcgca
    70681 attttcagct ctaccgtcgc ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca
    70741 cgctgtttgg cctgcaaact ggcgggcgca ccgagtcgat cctgatgtca atgccgccac
    70801 tggtacgctg ggagtatgat tatcagccaa aagatggcag cccagaagcg gcgttaagtg
    70861 agtttattaa ggtcagggat tgggtgtaac tccctcaccc ccattcccgc atccgctgat
    70921 gcaacgtcaa cgacggcttc tcggcaaaca gctgctggta atccgtggca aattgcccca
    70981 gatgccagaa tccccactgc atggcggcgt cttttaccgt tgtgctttgc gaccacgggc
    71041 ttatcagttc gcggcgtacg acgttcaggc gaatgcgttt cagccacgcg tttgggccaa
    71101 tgcctaaaat agcgtgaaac gcgttttgta gcgtgcggcg actgacatgc agttgattac
    71161 acaaatccag caccgtcacc ggttcggaca tgttttccag cacatattca cgggcgcggg
    71221 aaagcaaccg acggtaactc tgatgactga tgctttccgc cgtcaccatt ggttgcgctt
    71281 cttccagcat cgcccccatc gccattagca aattatcccc cagcactttt cgcactgcag
    71341 gctgatggag attttccggg ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc
    71401 acagcgcggc tttatgctgc tctttcactt ccagcgccga ctggctacgc agcatatgta
    71461 atacccgatc cggattgtgc aaaaagttag cctgacaggt gatgacatct tcagacagca
    71521 ctacgcccag gatcgtataa tcgtccggcg tacttaattc aaactcggtt ccaccggggc
    71581 gcgtcgctat ttctgcgctt cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg
    71641 tcgccggaat gccaaaccag aacgagttcg gccagaccag gcacgactga cgcagcgcca
    71701 gaccggtgta ttcacgaaaa acctgaatat catcgagtag aatttccgta aattcaccat
    71761 gaaacttgcc cggatgcagc tgatcgtaaa tctgctgcta ggcggtaatc gtcaaagcat
    71821 gttcatagac atccgttgtc tgtcgttgat gaacattatc cacttcgacc ttcggcgtga
    71881 gcttcaggtt ttcgggtaag ggttcatgat aaagatggtg caaattggct gtacgggtct
    71941 ttttcatgat gttaatgccg ggtgttgtag gacacccggc acctccgaca ggttaatggg
    72001 gcttgagacg ataacgacta ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga
    72061 gcgcatttct ggctttttcc agcggccagc caaagtgggc ggccacttct cctgccgtca
    72121 ttccctgaca tactgatgtt aacagcgcca acaactcgtc agcagattcc gcgacgataa
    72181 ctggcgcgtc gggttccttt acgggttgct tcggctggcg gttaaagcca gtaaccagcc
    72241 actgggtgtc atcgtctggc cgaccgatct ccttacggct gatgacacgc ccggtacgca
    72301 tcgcggcagc acaacctgcg tccagcgccg cacgacacgc cgccagatcg ccttccacca
    72361 ccagcgttaa gcgaccaggg tcaagcactt cgtgactgag cagacgaacg ttagctgctt
    72421 tgagcatggc atccgctgca tctatcgcgg cgaccatgcc gtccacttcc agcaatccca
    72481 gtgcattgat catcggcaac ctccgttacg cacgctggat tggattacgc gcgatttcca
    72541 gcactgcatc ggtaaaggcg ttacaggctg ctttacacgc ggcctggcta ccggttaaaa
    72601 atgctgccga gtagttggtt tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg
    72661 cagatttcaa cgcggcatca atgccgtagg tcgcttccag cggcggtgcc accagatacg
    72721 ccatcggatc gccaagcgtg atcccggcgg ttgatgaaag ataagaaccg gtacgcgaaa
    72781 ccacatgcgc caggaaggcg gtgttttctg cgtcattggc ccactggaaa gctgcgccat
    72841 tttcaatatg tgcaaccatc gcatccagac cggcacgcac ttccgccggg tttggcccac
    72901 caagcataat cagcacttca ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca
    72961 gcgagcgacc atataccact tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt
    73021 aggttacgtc gtcagaatcg gcagaaatca gcccgaggct acgaatatgc ggcggcaatt
    73081 ttagttcacg cgcaaattca gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg
    73141 gtcgaatcaa atctaaagct ggcatgatgc ctccttatcg ggtcatgttg atgccggatg
    73201 ctttctgctc cagcatacgt ttcgccaaat ccacaatgac ggcacagcag cacgaaagag
    73261 aagcaaaagt cagctacctg gtacactagg cagcgggtca gcaaaggggg gcaaatgccc
    73321 cttttttcat gtcagataat cagatcatta cctcaataaa ctaataatca catcgcggtt
    73381 acaaccagtt tgatagtgat agctcaaacc cagacgtggc gctggctgca agcaaaataa
    73441 gccgcaaaaa tgcgacccca tgtttactag ccgcaaaaat gcggtcctat ttgccagaac
    73501 cttttattat tcaattggtt agtgcaagaa aaagcgtgat tgctgttcaa gaagtatcca
    73561 gaaaggtcat gattttgacg ctgaaaaaaa ataggtgggc atttttgccc actcactagc
    73621 cgcaaatttg cccactcact agccgcattt ttgcggccca aagtaataaa tacagagaaa
    73681 taataaatac agatcagcgc caagattttt cgtcttttcg gcggaatcat gagactagtg
    73741 cttacgtcct gataacctcc agagcagacc ttaacttctg tgcagcagga ctacgctcat
    73801 tgccgctcgg ctaggtcaaa tcctcacgat aaagctgttc ggattgtgac cataatgtta
    73861 aaggactctt taaatcattc cttgacgaat taactagctg gcttaagcgg aatagcatgc
    73921 aggataaaaa caaaaccagc ttagcagtgg ggagagcttc gcttcgctcg tctcctgggg
    73981 ccaatgctca atgaaacgac gtaaggcacg gagcgctcac cataggtaga cacataaaca
    74041 tctgagagcg caccttctat cgcgctacgc gctcaataaa aaccaacgac accgccacac
    74101 tgcaagacca atggttttta tcgcgtatac agaccagaca ggaaacagca gcaaagcagc
    74161 gtatagccag gtgcagacag cacactcaca cgacgcacag caggcttaag tgatcactca
    74221 ggaaacttta tccccgccgc cgatcaccgc gaactatcat gaccgcgcca aattcgaaag
    74281 gaatggccca tagaacgcga tgaacacaca taagcgctac acttacttaa cgtcgccaca
    74341 gagcgattga gagcgcttaa aatcgatgcg atttcattaa atttttctca ctcgcctgaa
    74401 tggtggcgat caggactgat cgcaacattg ccccgcctcg atattgagcg cataatagat
    74461 acatacatta agcggaaaag agataagttt attgtgaaaa tcagggggtt acatatcatg
    74521 cgcatatcag aacaacaacg gcagatcctc gactatctgg caaaaaacga atcaatcacc
    74581 gcacgacaag ccgcagatct cgtttatggt ggcaatgtta cccgcgtcca ggttgacgcc
    74641 gcccgccgct cactcatgac gatggttagt aatgggctaa tcaggaaaca aggccgcgct
    74701 ttcgtagctg aaagacatat caatcacgct tatagccagg acgttgaaga attgcgccgc
    74761 cagatccttt gggtattcag caaacagggt caggagtatt tcctttctga acacccaaat
    74821 ttcgccgcca aagagttaac agtaagcgga ttgtcgtgcg cactggatta tcttgtcggt
    74881 gaaggttgcg ttacttacaa gcagctatgg cacgccctga atacgcttgt agaagatggt
    74941 ttactcattg ccacgcgtga gaacatcgaa tactggataa tgccgcgttc aggtcgccgc
    75001 aatcatgcac ggtatatttc aggcaacaga tacagaacgc cggactttaa tccgacacag
    75061 gaagatcggg accgtgccga aatgatgtgc agggaacagg agaaaagaat tatggataag
    75121 ttattttcga tgcagtgaac acaagcgggc gcaatgcccg tttttattgc cttattccac
    75181 gcctgacgaa tcaatttgat cacacctgag aaaatgcata gccagaagtt aaggccgtta
    75241 tattgcgatt gggagcgttt taaacggtat caatttaatt aattctgatg tggtgatttg
    75301 caggcaaaaa aaacccgccg gagaagcggg aaatagagac atatcgttgt agttattaac
    75361 agatcacttt gaggtcgagg gcggctaacc ctcgctaata ttatcccgct attaccttgc
    75421 gggtgcaaat aatcttttat tgctttgatg atttaataaa tagcactttt tgctaacaaa
    75481 aaccgctgtc aatatatggc caaaaatgtc aacagaaaaa ataaggttta taatattaag
    75541 tatcacatgg gtaatgggtt cagggaatcg gaatccaccg cctcacggaa attcaaaatt
    75601 tgcttcttct ttcctccgtg ggcggcttct ttttcgtatc gaggtattta tgaaagcaat
    75661 aacattagaa cagttcgcag caatggcgaa tgtatcgacc agaacgctga ggcgctggct
    75721 aaaaaaacca tcaaccggag tccctttgcc atttacccca cgcggtactc gccaattatt
    75781 ctgggagcac gaatgcaaag aatggttgta cagaacagaa cgataataat cacgcatcac
    75841 attttgaggt aaacatcatg accaatatcg aaagattaga acgctgtgcc gatattatgc
    75901 gacgcagatg gatttatgac cctgaagcgg gtttattgtt tagccgtgaa acaaaagacg
    75961 ttatcaaagg cagcatgacc aataaagggc atttggttgt aacggtacat gaaggtaaat
    76021 tcaaagtgac actcacctat cagaaagcct gctatgtata cacatacggc gcttatgacg
    76081 agacattata tgaagtgcac cacgttaatt tgaacaaaca ggataaccgc atcaagaata
    76141 ttcgcctgat gccgaaagaa aagcaccgcc ggatacataa aaacgtccgc aaacttattc
    76201 ggctgggcca cacacaatgg cttcaaccgc aattaattga acaatcacta taaacacagg
    76261 agtaaatacc atgcattcat tagacagtta ttttcagcgc acgacagccc ctaaatcagc
    76321 cgcccaggaa cgccgtgagg aattccatga gaaagtgatg cgttctgctg attatattgc
    76381 ggacaagttc gtggaaacgg ttcgtccact tgttgatgaa gtagctgata aacttcagtc
    76441 tgaaatgccg gaagatatgg aaggcactgc aaagcggcga ctcatttgcg aattaagtcg
    76501 tcgttttggc gtgtcaataa gcgcttttaa ataatcgaaa aaagggggga aagataatgg
    76561 acgcatttga ggtactggca aataaaggcg ctgaactggt agcacagcgt gataaaaccg
    76621 ccaacgaagg ggaacgcgca ctgctaaata aacagattaa agcgatccgt atggcccagt
    76681 ttaaattaat cagtaatgaa gttatcgaaa caattaaacc gcagatccca caaatcgttg
    76741 ctgacgctat taaagccgcc ggactggaga aacgcattgc cgggctaaaa acgaacgcag
    76801 agaaaatgga gatgtacaaa cagagcggtg ctccgaatcc cgatgagtac ttctcaccgg
    76861 atgttgagtt tatggcgcag gttgaggaac gtctgggtag ctgtctcacc gaggaacagc
    76921 gtcgctattt tgatggagtg gatagttcgg caggtattga cctgaactcg tatttcggtc
    76981 gtgaaattga gcattttgat gccgccccgt ctatgccgga gccagaacca gaaatggcca
    77041 ccgatcctga agctgagcaa cggaaacgcg tattcaatgc gatagggatt cggtactgat
    77101 cggataaaag aaagccggga attaccccgg ctgacttaac aatgtgacca atgaacaaat
    77161 cggacaagac aaacaggata agcagtctcg aaacaatgtt cgggaactat attagcacaa
    77221 gtccggagaa aatcatcatg ttggcactac gcatcatgca gggcatcgct aagaccctcg
    77281 cagaacatgt cttagacctg aaacattcgc cactgagcaa acaggccatg aagcgccaaa
    77341 cgcttcgtct gtgggctgaa tattcgcttg gaactatcaa taaaatcatc gacatgaaat
    77401 cggggccgag caaccagagc gcggaggaaa tggagttcat caggcgttta attctcattc
    77461 gcagagacat tcatagccag ttacacagcg tgggaatcga tattaatgat gggacgggcg
    77521 attaattttc ggacaagtac tatgcgcgcg cgaggagctg aacatgaagc aagatattgc
    77581 agacaggctg gagatactcg aaggccagcg ggcagaagcg aaacaactac gtaagcaggc
    77641 cagaagagcg cacaggaaca acgaagcaga gttactaacc aaatacatca gtttcaccaa
    77701 ttactgcatc tacgagtgtt acaaggaaga tgcagaggac tggctggatt cattgccaga
    77761 acagtactga gcagaataac accacgtcat gttgttctga ggtgagcata tcacctctgt
    77821 tcaggtggcc aaatttagtg tgccacttca gcatcaccgt tgaaatcaca ataacgattt
    77881 ttttgtaaat atgctctcac atatgtagga atagaccagg ttggcattga aacacatcta
    77941 ccatgaccat aataaagtaa ttttttgata tgtttatggt tttatattaa ttaaaatata
    78001 tctaaatttg atagttttaa aattgcaaaa tccagattgt gtttgtaatg tttaatctga
    78061 ttgtattgag tggcgtttat tatgaaaaat aaatcaggta attcttgtta tagagaagca
    78121 gtcgatattc atagctcact tgagctttca tttatattgc ataaatatca tttgcgtgta
    78181 gatgagaaga aatctcgaaa tgtattcagc gccatacgtg atgagctcat ccttgatggg
    78241 gattcacgac aaaacttagc tactttctgt caaacatggc ttgatgatga aatccgtgag
    78301 ttgcttaatt tagctattga taaaaacatg atttataaag atgaatatcc acaaatggcg
    78361 gaacctgaag gtcgctgtgt gcgtatgctt tccgattcgt ggagtttccc cgattcgcga
    78421 cacaccttag gttgctcaac cattggttca tctgaagctg ctatgctcgg ccgcttggcg
    78481 ttaaaatggc agtggtgtaa aaaacgcgaa gtacaaggta aatcaaccga acctgatttg
    78541 cgatcctgtg caaatatgct ggcataaatt ctgccgttac tgtgatgtga aattgtgtga
    78601 aattccactt gatgatcatc acctgataat gaatgctaaa gaggttttga agcatgtcga
    78661 tgaaaacact attggtgtcg ttacgacttt aggtattact tatttttgta aagcagattg
    78721 cgttagtgtt tttaagataa gtgttaaagt taatattagt ttttatattt acatcgtaat
    78781 tatatgtttc tattttaaaa atgttcgaga gtataaaaat gtctaatgct aatatcgcac
    78841 ccaaaagtaa gaaaatgaca gtggggacac tcgctatcat gaatataact gctgtagtaa
    78901 gtctacgatt tataccttct gaagcagaat acggcttagg tgccattttt tactatacct
    78961 ttgctgcaat tatgttttta gtacctgttg cctttgttgc ggctgagcta gctacaacat
    79021 atccacaaaa agggggggca tttcgatgga taggagaagc atttggggct cgctggggtt
    79081 ttgtaggaat gcttatgacc tggctacaag tcatccccta ctttccaaca gtactgacat
    79141 ttggtgcagt ctcggttgca tttattgatg ttgatatggg ggtggcagaa tcaattgcca
    79201 gtaataaatg gtatatttta tttttcgtac tatttgttta ctggggagct atttgtgttg
    79261 cattacgtgg cgttggtatt ttctcgcagg tatctaagtg gtgcggtatt atagggacaa
    79321 ttattccagc tattatcctt gttattcttg gtttctccta tctgtggttc agcggtaaac
    79381 cagcggtgat cccattaggt tggggtgatc ttttccctaa tatagctaac ttccagaata
    79441 ttgttcttgc cgccagcata tttcttgctt atgcagggat ggaaatgaat gcggtccatg
    79501 ttaacgattt agataatcca actaaaaaat acccaattgc aattaccatc gcttctatag
    79561 gtactattgc aatattcctg ctcagtactt taggggtcgc ctttattatt cctacagata
    79621 aaattagtct aactcaaagt ttgttggttg cctacgatat gctgttcgaa tgggctggag
    79681 tgccatggct tggctctgtg atggcattta tgctagcaat tggcgtactg ggtggggtgg
    79741 ttacttggat tgcagggcct aatacaggtg tattagcgat cgcgaaagct ggttatttac
    79801 ctaagttttt ccaaaaaacc aatcgtcatg gaatggggca ccatctaatg tttgtacaag
    79861 gtataattgt ctcagttctt tctgttactt ttgtgataat gccttcagtt caagcagctt
    79921 ttcagatttt atcacagtta acagttatgc tctatttagt tatgtatatg ctcatgttcg
    79981 ctagtgctat ctatctacga cattctcaac ctgatacaaa acgcccatat cgagcccctg
    80041 ctttattttt ctgggcaaca cttggattct tagggtcttt tctggctttt gtattaagtt
    80101 ttattccgcc cgagcaaatc ccaactggta gtccaaccag ctatgttctt tatttgttag
    80161 tattagttgc cctattttta ttaattccaa tattaattta cgccttccga aaatctgact
    80221 ggaaagatcc aaactctgat ttcgaaccat tttcttggga aaaaagaact agtaattaaa
    80281 ttttccataa gctacaaaag atgaggttaa atatgtttag tgctgaatct ccaattggtg
    80341 taatagactc aggtgtcggt ggattgacat cggtaaagga aataattaat ttgcttcctg
    80401 gtgaagatat tatctattgt ggagataata tgaatgcacc ttatggcaac cgttctgccg
    80461 atgatatcat cagtttgact aaaaaaatgc tgactttttt acagtcaagg aatgtcaaac
    80521 tagttgctgt cgcatgtaat actatatcct caactttgga atccgaagaa tacagcggat
    80581 atgctaagag ctttccgttt cctatcttat ccatcattga acctgcagta gaagatgtga
    80641 ttcgccagca atataaaaat gttggtatta tcgctaccga gtttactatt aaaacggggt
    80701 gtcacaaaga gctaataaaa aaattgaatt caacaataaa tgtatttgga gaaccaagca
    80761 aaaatcttgc aatgttaatt gaggaaggta atcttaatgc tcctgcaatc ttgaatgaca
    80821 tcaaaaaaca tgttaaccat ttaatttccc tccatccagt aaatgaaatt attctcggct
    80881 gtactcacta tccaattgtt cagaattttt ttgaagcagt tgcaccggat ataaaattta
    80941 ttaatcctgc acatgatcag gctatctcta ttaaaaatca cttgggacag ctaaatttat
    81001 taaactcctc aaatatagga actttgcaaa taaatactag tggaagtatg gagatctaca
    81061 aaacggtact aggggaattg agcattacga aacctcatac attcagtatc aggcagtttt
    81121 aaatttatcc ggtacaccgt aaaatcccgt ccttctgaac tgcaccccaa aagttggaca
    81181 ccaactgagt aaggtgcatt ttatggacaa acccagttac atactgggta gtgcatccaa
    81241 ttagtagaac atgtgttttt cgataaacgc tccgatcact ttttcgtgga tctcaactga
    81301 gcgtgagaag cagattgttt tacgacccaa ccgcttaatg cgggtgcgta gcgtcagatt
    81361 attacgctca atgcgttggg tgaatatttt gccggtcaga tgcttattct tcggcacctc
    81421 ccggccatag ctgccccagt catcgctggt cagcatgccg atgttgaagg gtgtaagcag
    81481 tgccagtagc tcccggcacg tttgatcggt tcggggacca aaagtgtagg ccagtacacc
    81541 gcctgttttg gtgttgtacg cgtacccgag ccaatgttgc cgggctttac tgccaacgta
    81601 gctccattgc tcatcaagct cgcagataaa cgccacatca gcatgggcaa caggcgaaga
    81661 agttattcgc tttggcgcga gttttttaaa gtccggatga cggtgttact accaattttc
    81721 agtgtcctgg cggtatcgcg aaccccggca ccattaaagg ccatttcagt tatcagctct
    81781 ttcatacccg gcttacgtgc ttgataagta taagtgaact gaaacacacg gtggcagtca
    81841 cggcagcgaa gtctgtcacg gcctttaggg ttctgaccat ggcggtaaac ctgagctgac
    81901 tgacaacggg gacaatgaat attaacgctg gtcatgagat aacctcaaaa gcccgtatta
    81961 tacatcagat tcaactaatt agaggcatca cccatccagc attataaaac atactaacaa
    82021 taactaaatt ataataatca caacttacaa tcaaataata aatgcataag attaacaatg
    82081 attttttcgg gttatattta tctaaaaaag cagaatatta tgtaacttat tattttctaa
    82141 gcgaaaattt attgtgcggt cagactaaca aacttgcgta ccacccgaag aacatgcgaa
    82201 gatttaagta ttctttcttt tttggttttt atttttcatt ttctcttttc ttctctttcc
    82261 ggttctgttc tggttttggt tttttccttt ttatttttat tctttcttca gggttatgct
    82321 aaaacaccaa aacatttttc accacaaaag catccaatca tcttgccaga ccaatcacct
    82381 taaccggata cactgccgga tataatccca ctaacaccca gacaccaagc accatacctg
    82441 ataactaccg tattcaggaa ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc
    82501 cgccaaaaag aggcgtacca atgtcgatat agtcccccgc gcggacaatc acttcgtcaa
    82561 tgactgccaa cgggagttgt tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa
    82621 agtcctgccc ggccaccacc agcaggggat gcggattcgg aaaacgcgcg acgaaatcga
    82681 ccagcgcgtt gatgaccgtc agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg
    82741 ccagcacgta cacgtcagtt ttgggatcaa gatccagctg aagcagcgcc tgttgccagg
    82801 cactcaccag atccgtttca tcaatcggga tcgccaccgg caaattgcgc agcggcagtt
    82861 gtacgccctc cagccagatt gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa
    82921 tcaccgtgga gcgtacggtt tgcgccggaa actgcacatt catctcacgc aggcgcggat
    82981 ggtcatgcag cgccgttgcc agaagtgggc caatatcggc aaaacagaac gggttggcgg
    83041 gctggtggcg ataacattcg cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac
    83101 ctgcgggcag caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa
    83161 tcacttcgac aatcagcgcc gccatccgcc gggtaacctg caccagctgc gcgccggtca
    83221 gcgaacgggc gtcagtgcct gcaccgaagc actcatccac aatcatctgc cccggtttat
    83281 gagcgtaaac cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgagac
    83341 aggcagtgcc gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga
    83401 tatcgatatt cagtacccga cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt
    83461 gaccggcgat cacggattcg aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg
    83521 actgtttttc cttcatcact ttttccatca gctgtgcgac caggctttcg gtaaactggc
    83581 cttccggcag ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg
    83641 gcacgctggc gggcacggac gccgttaccg ggatcgattc atcacattcg gtgatggtaa
    83701 aacccagtag atccgccact tcacgggctt ccggggtaat aatgctggcg cgcagaacca
    83761 cggacattgc ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt
    83821 ttttcacctg cttcctcctg tttggtgaga gatgccagat aatccaccag ttgctgcacg
    83881 ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat
    83941 aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg ttttactgat gacggcgatt
    84001 tgccgtttgc tgacgccaat atccagcaac ccggcaggca accgactttc cgggtcattt
    84061 gcgccatgga catagatcag catgtctaca tcctgtagcg tggtaattaa ggcgtgatac
    84121 cagcggggat ggctaaaata ttcacccggc gtatcaatat cgcccttatc attaaattcc
    84181 acggccagtg tttttctggc gagggtataa ttcccctgca acgcattaaa taacgttgtt
    84241 tttcctgccc cgacgctacc cacaaacgca attcgtttca tgaatgcctc gtattaactt
    84301 ttggtcattt cacaaagcgt ataatttaat aagcgaccta aaccgctgac ggtttgtgat
    84361 aacgcttctt ctactgcgcc caccgagcca taaatcacca atgcaccgct gaagcggtcg
    84421 agaaaaccga tatgcacatc agcggctttc agcgctaaat caccggcgat catcgccgtt
    84481 tcgccgggag ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc gattttcttc
    84541 gccagttcct caccgggatg cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca
    84601 aattcctgaa tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat
    84661 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
    84721 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
    84781 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
    84841 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
    84901 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
    84961 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
    85021 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
    85081 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
    85141 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
    85201 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
    85261 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
    85321 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
    85381 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
    85441 cagcagcgca ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
    85501 caccacacta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
    85561 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
    85621 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
    85681 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
    85741 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
    85801 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
    85861 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
    85921 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
    85981 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
    86041 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
    86101 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
    86161 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
    86221 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
    86281 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
    86341 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
    86401 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
    86461 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
    86521 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
    86581 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
    86641 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
    86701 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
    86761 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
    86821 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
    86881 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
    86941 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
    87001 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
    87061 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
    87121 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
    87181 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
    87241 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
    87301 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
    87361 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
    87421 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
    87481 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
    87541 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
    87601 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
    87661 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
    87721 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
    87781 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
    87841 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
    87901 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
    87961 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
    88021 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
    88081 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
    88141 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
    88201 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
    88261 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
    88321 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
    88381 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
    88441 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
    88501 tatggatgct gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
    88561 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
    88621 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
    88681 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
    88741 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
    88801 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
    88861 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
    88921 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
    88981 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
    89041 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
    89101 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
    89161 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
    89221 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
    89281 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
    89341 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
    89401 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
    89461 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
    89521 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
    89581 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
    89641 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
    89701 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
    89761 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
    89821 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
    89881 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
    89941 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
    90001 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
    90061 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
    90121 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
    90181 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
    90241 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
    90301 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
    90361 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
    90421 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
    90481 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
    90541 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
    90601 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
    90661 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
    90721 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
    90781 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
    90841 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
    90901 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
    90961 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
    91021 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
    91081 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
    91141 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
    91201 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
    91261 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
    91321 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
    91381 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
    91441 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
    91501 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
    91561 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
    91621 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
    91681 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
    91741 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
    91801 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
    91861 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
    91921 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
    91981 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
    92041 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
    92101 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
    92161 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
    92221 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
    92281 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
    92341 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
    92401 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
    92461 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
    92521 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
    92581 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
    92641 ctggcatgac aaattcactg ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg
    92701 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
    92761 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
    92821 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
    92881 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
    92941 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
    93001 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
    93061 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
    93121 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
    93181 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
    93241 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
    93301 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
    93361 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
    93421 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
    93481 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
    93541 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
    93601 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
    93661 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
    93721 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
    93781 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
    93841 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
    93901 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
    93961 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
    94021 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
    94081 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
    94141 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
    94201 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
    94261 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
    94321 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
    94381 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
    94441 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
    94501 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
    94561 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
    94621 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    94681 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    94741 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    94801 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    94861 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    94921 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    94981 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    95041 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    95101 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    95161 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    95221 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    95281 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    95341 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    95401 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    95461 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    95521 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    95581 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    95641 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    95701 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    95761 acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc agttacgcga
    95821 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    95881 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    95941 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    96001 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    96061 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    96121 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    96181 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    96241 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    96301 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    96361 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    96421 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    96481 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctgacaa tcctgttgtg
    96541 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    96601 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    96661 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    96721 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    96781 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    96841 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    96901 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    96961 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    97021 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    97081 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    97141 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    97201 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    97261 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    97321 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    97381 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    97441 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    97501 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    97561 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    97621 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    97681 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    97741 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    97801 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    97861 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    97921 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    97981 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    98041 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    98101 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    98161 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    98221 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    98281 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    98341 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    98401 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    98461 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    98521 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    98581 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    98641 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    98701 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    98761 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    98821 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    98881 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    98941 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    99001 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    99061 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    99121 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    99181 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    99241 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    99301 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    99361 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    99421 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    99481 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    99541 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    99601 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    99661 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    99721 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    99781 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    99841 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    99901 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    99961 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
   100021 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
   100081 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
   100141 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
   100201 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
   100261 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
   100321 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
   100381 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
   100441 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
   100501 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
   100561 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
   100621 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
   100681 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
   100741 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
   100801 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
   100861 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
   100921 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
   100981 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
   101041 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
   101101 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
   101161 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
   101221 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
   101281 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
   101341 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
   101401 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
   101461 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
   101521 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
   101581 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
   101641 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
   101701 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
   101761 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
   101821 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
   101881 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
   101941 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
   102001 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
   102061 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
   102121 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
   102181 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
   102241 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
   102301 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
   102361 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
   102421 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
   102481 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
   102541 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
   102601 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
   102661 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
   102721 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
   102781 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
   102841 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
   102901 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
   102961 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
   103021 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
   103081 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
   103141 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
   103201 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
   103261 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
   103321 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
   103381 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
   103441 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
   103501 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
   103561 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
   103621 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
   103681 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
   103741 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
   103801 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
   103861 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
   103921 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
   103981 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
   104041 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
   104101 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
   104161 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
   104221 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
   104281 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
   104341 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
   104401 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
   104461 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
   104521 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
   104581 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
   104641 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
   104701 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
   104761 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
   104821 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
   104881 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
   104941 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
   105001 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
   105061 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
   105121 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
   105181 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
   105241 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
   105301 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
   105361 tgcctgaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
   105421 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
   105481 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
   105541 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
   105601 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
   105661 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
   105721 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
   105781 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
   105841 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
   105901 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
   105961 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
   106021 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
   106081 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
   106141 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
   106201 actagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
   106261 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
   106321 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
   106381 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
   106441 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
   106501 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
   106561 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
   106621 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgccgac gcacacgttt gcgtatcata
   106681 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
   106741 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
   106801 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
   106861 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
   106921 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
   106981 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
   107041 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
   107101 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
   107161 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
   107221 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
   107281 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
   107341 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
   107401 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
   107461 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
   107521 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
   107581 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
   107641 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
   107701 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
   107761 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
   107821 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
   107881 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
   107941 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
   108001 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
   108061 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
   108121 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
   108181 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
   108241 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
   108301 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
   108361 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
   108421 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
   108481 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
   108541 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
   108601 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
   108661 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
   108721 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
   108781 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
   108841 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
   108901 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
   108961 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
   109021 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
   109081 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
   109141 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
   109201 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatctgc ggacgcagca
   109261 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
   109321 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
   109381 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
   109441 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
   109501 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
   109561 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
   109621 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
   109681 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
   109741 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
   109801 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
   109861 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
   109921 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
   109981 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
   110041 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
   110101 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
   110161 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
   110221 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
   110281 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
   110341 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
   110401 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
   110461 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
   110521 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
   110581 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
   110641 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
   110701 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
   110761 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
   110821 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
   110881 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
   110941 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
   111001 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
   111061 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
   111121 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
   111181 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
   111241 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
   111301 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
   111361 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
   111421 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
   111481 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
   111541 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
   111601 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
   111661 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
   111721 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
   111781 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
   111841 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
   111901 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
   111961 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
   112021 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
   112081 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
   112141 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
   112201 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
   112261 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
   112321 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
   112381 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
   112441 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
   112501 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
   112561 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
   112621 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
   112681 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
   112741 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
   112801 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
   112861 cgggccgggg atctggcaga attatcgcga tatccacaaa ctgtttaaac gccaggaagt
   112921 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
   112981 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
   113041 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
   113101 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
   113161 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
   113221 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
   113281 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
   113341 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
   113401 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
   113461 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
   113521 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
   113581 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
   113641 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
   113701 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
   113761 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
   113821 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
   113881 tcaacgtggg ggatgtggca ttatttggcc tggtcattga tcgcgtgagt acgctgattc
   113941 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
   114001 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
   114061 ttatcggcgc gatggcggga ctggtactct cctcgacgct gctcggtcag ttgttgtttt
   114121 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
   114181 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
   114241 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
   114301 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
   114361 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
   114421 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
   114481 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
   114541 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
   114601 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
   114661 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
   114721 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
   114781 tgcgtggcgt attgcacacc ctgccgttgc caggcgtgga tttctgcgtg gcagcgctgg
   114841 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
   114901 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
   114961 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
   115021 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
   115081 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
   115141 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
   115201 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
   115261 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
   115321 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
   115381 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
   115441 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
   115501 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
   115561 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
   115621 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
   115681 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
   115741 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
   115801 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
   115861 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
   115921 gcttagggat cacactgctg ctgattctag cactctgggt ggtccaaact gccgctgatg
   115981 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
   116041 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
   116101 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
   116161 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
   116221 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
   116281 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
   116341 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
   116401 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
   116461 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
   116521 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
   116581 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
   116641 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
   116701 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
   116761 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
   116821 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
   116881 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
   116941 tgccatttac cgccgtgctg tttggtggcg gtgcgctggc gctggcgctg gcagggatgc
   117001 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
   117061 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
   117121 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
   117181 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
   117241 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
   117301 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
   117361 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
   117421 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
   117481 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
   117541 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
   117601 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
   117661 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
   117721 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
   117781 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
   117841 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
   117901 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
   117961 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
   118021 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
   118081 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
   118141 accaactcgg ttgaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
   118201 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
   118261 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
   118321 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
   118381 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
   118441 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
   118501 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
   118561 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
   118621 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
   118681 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
   118741 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
   118801 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
   118861 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
   118921 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
   118981 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
   119041 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
   119101 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
   119161 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
   119221 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
   119281 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
   119341 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
   119401 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
   119461 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
   119521 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
   119581 gcaaacaacg cgcaacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
   119641 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
   119701 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
   119761 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
   119821 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
   119881 atttactggc gcagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
   119941 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
   120001 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
   120061 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
   120121 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
   120181 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
   120241 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
   120301 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
   120361 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
   120421 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
   120481 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
   120541 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
   120601 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
   120661 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
   120721 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
   120781 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
   120841 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
   120901 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
   120961 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
   121021 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
   121081 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
   121141 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
   121201 ctggctggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
   121261 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
   121321 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
   121381 ccatcatttt ttcggtctgg cttcagtcag tatggtactg ggcgatcatc gaaagaacga
   121441 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
   121501 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
   121561 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
   121621 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
   121681 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
   121741 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
   121801 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
   121861 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
   121921 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
   121981 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
   122041 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
   122101 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
   122161 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
   122221 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
   122281 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
   122341 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
   122401 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
   122461 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
   122521 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
   122581 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
   122641 ttcagcgctt cgcagttcaa tggcgtagtt gctgcacccg acgacgccag agaatgacga
   122701 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
   122761 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
   122821 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
   122881 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
   122941 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
   123001 gtttatggcg atgtccgctg gcgtgtttat ggcgatcgga tttacttttt acctttccgt
   123061 tatcgccgat gccccgtctt cataggcatt aacccatctg gtgggcggcc tttgctttac
   123121 acttggcttt attttgctgg cggtttgcgg caccagcctg ttcacctcgt cggtaatgac
   123181 ggtaatggca aaaagtcggg gcgttattag ttggcgaact tggctgatta acgcacttct
   123241 ggtggcctgc ggtaatctgg caggtattgc ctgtttcagt ttgttaatct ggttttccgg
   123301 gctggtgatg agtgaaaacg cgatgtgggg agtcgcgatt ttacactgcg ccgagggcaa
   123361 aatgcatcat acatttactg aatctgtcag cctcggcatt atgtgcaatc tgatggtttg
   123421 cctggcgctg tggatgagtt attgcggacg ttcgttatgc gacaaaatcg tcgccatgat
   123481 tttgtccatc accctgtttg tcgccagtgg ctttgagcac tgtatcgcca atttgtttgt
   123541 gattccgttc gccattgcca ttcgccattt cgcccctacc tccttctggc aactggcgca
   123601 cagtagcgca gacaattttc cggcactgac ggtcagccat tttattaccg ccaatctgct
   123661 cccggtgatg ctgggtaata ttatcggcgg tgcagtgctg gtgagtattt gttatcgggc
   123721 tatttattta cgtcaggaat cctgaaaaat cagcccggcg aaacagttcg tcgggctgaa
   123781 gattattctt gcgcgatttg cccatcgggc caggcgtgga tcacggcttt gatcagcgtc
   123841 gccaatggaa tggcgaagaa tacgccccag aatccccaca aaccaccgaa gatcaccacc
   123901 gataaaataa tcaccagcgg atgcaggtta accgcttcgg agaacaacac cggtactagc
   123961 aggttgccgt ccagcgcctg aataatcaga tacaccgcga agcagctcca gaattcagta
   124021 cctgcgccaa actggaatag cgccacgcca accaccggaa tggtcaccac aaatgcgcca
   124081 atgtacggaa taagaaccga gaagccgacc agcactgcca gcagcagcga atagttcagc
   124141 ccgaagagca agaaccccag ccaggtggcg atccccacca cgatcatctc cagcactttg
   124201 ccgcggatat agttggtgat ttgttgattc atctccttcc acacctgtcc tgccagccca
   124261 cggttacgcg gcagcacccg gcgaacggcg ttcagcatct gctctttgtc tttcagcagg
   124321 aagaagacca tcaatggcac cagcaccaga tagacggcta tggtcagcaa accgaccagc
   124381 gaggcgaggg aaattttcac caccgaatcg cccatggtca gcatccgact gcgcatattt
   124441 tcggccattg catcaataat gcccgcgtcc attaacaccg gatagcggcg cggcaacgtg
   124501 gcggcaaagt cagaaagctt attgagcatc cccggcatat cgcggattaa gtagatgccc
   124561 tgttgccagg cgataggcag taccacgaac gccatcagta gcaatatacc gacaaaaacc
   124621 accaatacaa tcgacgtcgc ccagcggcgg gagcagccaa tagattgcag gcgcacggtt
   124681 ggccattcca gcaaataagc cagcacaata gccaccagca acggagcaag caggccacta
   124741 aagaaaaaga taatgccaaa tccggcaact aaaataacca gcaaggcaat cgcttccggg
   124801 tcgctaaaac ggcggcgata ccattgcatc aacatttcga gcataaagac cttccctgaa
   124861 cctcaagagc gggattgcga tccgcaattg tatcgaaatg tcacaaaaaa gacttcgctt
   124921 tttatgacgg attcaggaaa ctgaaaagtc atttgagtgg gctaatcttc gccgttacac
   124981 tcaaaggcgg cgaggtggga acgatatttc acagtatcgg tcaaatgact atcttcagaa
   125041 atacaggata gaggttatgt tcaggcagtt gaaaaaaaac ctggttgcaa ccctcattgc
   125101 tgctatgacc attggtcagg tagccccggc gtttgccgac agcgcagaca ccttgccgga
   125161 tatgggaacc tccgcaggaa gcacgctttc cattggtcag gaaatgcaga tgggcgacta
   125221 ttatgtccgc cagctacgcg gcagcgcgcc gttaattaat gacccgctgt taacgcaata
   125281 tattaattcg ctggggatgc gtctggtttc gcatgccaat tcggttaaga caccgtttca
   125341 tttttttctg atcaacaacg acgaaattaa cgcctttgct ttctttggcg gcaacgtggt
   125401 gctgcactct gccctgttcc gttattccga taacgaaagt caactggctt cagttatggc
   125461 gcacgaaatc tcccacgtca cccaacgtca cctggcgcga gcgatggaag atcaacaacg
   125521 cagcgcgccg ctgacctggg tcggcgcgtt aggttctatt ttactggcga tggccagtcc
   125581 gcaggcgggg atggcggcgc tgaccggtac actggcggga acgcgccagg ggatgatcag
   125641 tttcacccag caaaatgaac aggaagcgga ccgcattggt attcaggtgc tgcaacgctc
   125701 gggattcgat ccacaggcga tgccgacctt cctggaaaaa ttgctcgatc aggcgcgtta
   125761 ctcctcgcgc ccgccggaaa ttttattgac tcacccgttg ccggaaagtc gtctggcaga
   125821 tgcccgcaac cgtgctaatc agatgcgccc gatggtggtg cagtcgtcgg aagatttcta
   125881 tctggcgaaa gcgcgcacac tggggatgta taattccgga cgtaaccagc tcaccagtga
   125941 tttgctggat gaatgggcga aaggaaacgt tcgtcagcaa cgagcggcgc aatatggtcg
   126001 tgctttacag gcgatggaag ccaataaata cgacgaggcg cgtaaaacgc tgcaaccgtt
   126061 actggcggca gaacctggca acgcatggta tctcgatctg gctaccgata tcgatcttgg
   126121 gcaaaacaaa gccaatgagg caatcaatcg cctgaaaaat gcccgcgatt tacgcaccaa
   126181 tccggtgttg cagctcaacc tggcgaacgc ttatctgcaa ggcggtcaac cacaagaagc
   126241 ggccaatatt ctgaatcgct acacctttaa taataaagat gacagcaacg gctgggattt
   126301 gctggcacag gcggaagccg cgctaaataa ccgcgatcag gagctggctg cgcgagcaga
   126361 aggttatgcg ctcgccggac gactcgatca ggccatttcg ctgttgagta gcgccagttc
   126421 gcaggtgaaa ttaggcagcc tgcaacaagc gcgttacgat gcgcgcatcg accagttgcg
   126481 ccagctgcag aaacgcttta agccttatac caagatgtaa ttcgcattaa aggaagaaca
   126541 atgaccaaac aggtaaaaat ctaccataac ccacgctgtt caaagagccg ggaaacgctg
   126601 aacctgctga aagaaaacgg cgtggagcca gaagtggtgc tctaccttga gacacccgcc
   126661 gatgcggcaa cgctgcgcga tttgctgaaa atgctgggga tgaatagcgc ccgtgaattg
   126721 atgcgccaga aagaggatct ttataaagaa ctaaacctgg ctgacagttc gcttagcgaa
   126781 gaggcgctga ttcaggcaat ggtggataat ccgaagctga tggagcgccc gattatggtc
   126841 gcgaatggca aagcgcggat tggtcgtcca ccggagcagg tactggaaat cgtgggttaa
   126901 gatgtgctcc atcgctagtt gaagcacatt gccggatgcg acgcttgcca cgtcttatcc
   126961 ggcctacgaa tactatggat taacttcgcg taggtcaggc gttcgcgccg catccgacaa
   127021 taaacacctt atctacaact tcagaatttc tttcacaaac ggaatggtca gcttacgttg
   127081 cgcggtaatc gacgcacgat ccaactgatc caacgtcata aatagcgtgc gcatttctct
   127141 gtcgagccgc ttcagcaaga aacgccccac atcttccggc agttcaaaac cacgcaaacg
   127201 cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa agtggctgca atttgtagat
   127261 ctgcccccag tcgagacgcg acgcgagatc cggtaatccc agattcaact gccgcggtgg
   127321 acgatcgccg gtgatcaaca accgtgtttt gcccgattcc agaattcgat tgtagagatc
   127381 gaaaatcgcc atctcccaca actcatcgcc tgcaatacac tcaatgttgt cgatacagac
   127441 cagcgacaaa tgctccatac cgtcgagcac ttccggaaca aaccaggtgc gtttatccag
   127501 cgggacatag cccaccgcat cgccacgctg cgacaattcc gcgcaagccg cgtgcagcag
   127561 atggctgcgc cccgcgcctt cgcgtgccca gagatagatg taaccgctat gttcctgacg
   127621 cagcacgttt tgcagcgcgg ccagtaaaga ggagttatcc cccggccaga aacttgcaaa
   127681 ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt gccggtgtgt tcagagatac
   127741 ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca gaatcccatg atgtttgaac
   127801 cgggcagcaa cactgcccgg tcggtacatt atttgtctgt tatatccgcg tcttctgcgt
   127861 ccagcaccac ttcttcaggg cgcagcacac tgatcagttt gaaaatcaaa cttaagccga
   127921 taccgacgat ggtcgccagc gccatacctt ttaactccgc cgcgccgatg tttaccttcg
   127981 cgccactgac gccgatgatc aaaatcacgg aagtcaggat caggttctgt gctttgttgt
   128041 aatccacttt cgattcgatc aaaacacgaa taccggaagc accaatgaca ccataaagca
   128101 gcagcgaaac gccgcccatc accggcaatg ggatcatctg gatagcggca gccagtttac
   128161 cgacgcagga aagcaggata gcgaaaatcg ccgccccgcc gataacccag gtactgtaaa
   128221 cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt ggtatttggc gtagagccaa
   128281 agaagccgga gatcacggtc gacaggccgt tagcaaacat cgaacggtgc agacctggat
   128341 cgcgcagcag atcttttttg acgatattag ccgttactac caggtgccct acgtgttcgg
   128401 caataaccac taacgccgct ggcagaatag tcagaatggc aaaccactcg aagcgcggcg
   128461 tatagagggt tggcagtgca aaccagtgag cattaataat cggcgtggta tcgacaattc
   128521 ccattgcgaa agagagcgcg taccccacca gcacgccaat taaaatcggg ataattgcca
   128581 ggaaaccacg aaacagcacg gaacctaaaa ccgtgaccgc cagggtggta atagagatga
   128641 tgatggtttt ggagtttggc gtttgccctt cagccgggag taaacccgcc ataccggcag
   128701 ctacgcccgc cagctccaga ccgatgacgg caacgattgc gcccattgcc gcaggtggaa
   128761 acagcacgtc caaccagccg gtccccgctt tcttcacgat aaaagaaacc aggcagaaca
   128821 gcacgccgca cataataaag ccgcccagcg cgacttcata ccctaacggc aacagtaaca
   128881 ataccggtga aataaaggca aagctggaac caagataagc cggaattttc cctttacaga
   128941 tgaagagata cagcagcgtt ccaataccgt taaataacag tacagtcgcc gggttaatat
   129001 gaaataagac gggcaccagg acggttgcac caaacatggc gaacaaatgt tgcaaactaa
   129061 gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc gatagcacgg cgcgtcatag
   129121 tattatcctc tgtattatgt gttataggcg ctttactcaa aaaaaagccg actcttaaag
   129181 tcggctttaa ttatttttat tctttatttc gtaccaaaga ttttgtcacc ggcatcgccg
   129241 aggcccggaa taatgtatcc gtgctcgttc agtccctggt caatcgatgc agtatacagt
   129301 tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt ctggcgcagc gaccagcacc
   129361 agaactttga tgctgctgca gcccgctttt ttcagcaggt cgatggtcgc gataacggaa
   129421 ccaccggttg ccagcattgg gtcaacgatc agcgccatac gctcatcgat gttagaaacc
   129481 agtttctgga agtacggtac cggctccagc gtttcttcat tacggtacat accgacaacg
   129541 ctgatgcgcg cgctcggaac gttttccagc acaccgtcca tcataccaag acccgcacgc
   129601 agaattggca caacggtaat tttcttacct ttgatctggt cgatttctac cgggccgttc
   129661 cagccttcga tagttacttt ttccgtttcg aggtcggcgg tcgcttcgta agtcagcagg
   129721 ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc tgatatcttg ctcacgcatc
   129781 agtcccagct tgtgtttgac gagtgggtgt ttgacttcca cgatcttcat actctttctc
   129841 ctttgagggg cagccacaaa aaaatcgacg gattatacct cctttcttca gggcggcaat
   129901 attattttcg ttgactttag tcaaaatgat aacagtttga gataaagtta ttttatattc
   129961 aaatggttat gaaagaagat tattccatcc gaaaactaac ctttgccctg gcacaagtct
   130021 tctttcgccg cgcgcccggg gaaaagacgt gcaaaaaggt tgtgtaaaac agtctcgcaa
   130081 acgtttgctt tccctgttag aattgcgccg aattttattt ttctaccgca agtaacgcgt
   130141 ggggacccaa gcagtgaccg ataaaacctc tcttagctac aaagatgccg gtgttgatat
   130201 tgacgcgggt aatgctctgg ttggaagaat caaaggcgta gtgaagaaaa cgcgtcgtcc
   130261 ggaagtgatg ggcggtctgg gcggcttcgg tgcgctgtgt gcattgccgc aaaaatatcg
   130321 tgaacccgtg ctggtttccg gcactgacgg cgtaggtacc aagctgcgtc tggcaatgga
   130381 cttaaaacgt cacgacacca ttggtattga tctggtcgcc atgtgcgtta atgacctggt
   130441 ggtgcaaggt gcggaaccgc tgtttttcct cgactattac gcaaccggaa aactggatgt
   130501 tgataccgct tcagcggtga tcagcggcat cgcggaaggt tgtctgcaat cgggctgttc
   130561 tctggtgggt ggcgaaacgg cagaaatgcc ggggatgtat cacggtgaag attacgatgt
   130621 cgcgggtttc tgcgtgggcg tggtagaaaa atcagaaatc atcgacggct ctaaagtcag
   130681 cgacggcgat gtgctgattg cactcggttc cagcggtccg cactcgaacg gctattcgct
   130741 ggtgcgcaaa attcttgaag tcagcggttg tgatccgcaa accaccgaac ttgatggtaa
   130801 gccattagcc gatcatctgc tggcaccgac ccgcatttac gtgaagtcag tgctggagtt
   130861 gattgaaaag gtcgatgtgc atgccattgc gcacctgacc ggcggcggct tctgggaaaa
   130921 cattccgcgc gtattgccag ataataccca ggcagtgatt gatgaatctt cctggcagtg
   130981 gccggaagtg ttcaactggc tgcaaacggc aggtaacgtt gagcgccatg aaatgtatcg
   131041 caccttcaac tgcggcgtcg ggatgattat cgccctgcct gctccggaag tggacaaagc
   131101 cctcgccctg ctaaatgcca acggtgaaaa cgcgtggaaa atcggtatca tcaaagcctc
   131161 tgattccgaa caacgcgtgg ttatcgaata atgaatattg tggtgcttat ttccggcaac
   131221 ggaagtaatt tacaggcaat tattgacgcc tgtaaaacca acaaaattaa aggcaccgta
   131281 cgggcagttt tcagcaataa ggccgacgcg ttcggccttg aacgcgcccg ccaggcgggt
   131341 attgcaacgc atacgctcat cgccagcgcg tttgacagtc gtgaagccta tgaccgggag
   131401 ttgattcatg aaatcgacat gtacgcaccc gatgtggtcg tgctggctgg ttttatgcgc
   131461 attctcagcc cggcgtttgt ctcccactat gccgggcgtt tgctgaacat tcacccttct
   131521 ctgctgccga aatatcccgg attacacacc catcgtcagg cgctggaaaa tggcgatgaa
   131581 gagcacggta catcggtgca tttcgtcacc gatgaactgg acggtggccc ggttatttta
   131641 caggcgaaag tcccggtatt tgctggtgat acggaagatg acatcaccgc ccgcgtgcaa
   131701 acccaggaac acgctattta tccactggtg attagctggt ttgccgatgg tcgtctgaaa
   131761 atgcacgaaa acgccgcgtg gctggatggt caacgtctgc cgccgcaggg ctacgctgcc
   131821 gacgagtaat gcccccgtag ttaaagctcc agctctgccg ctggcgtttt tcaattcacc
   131881 tgtaaatcgc aagctccagc agtttttttc ccccttttct ggcatagttg gacatctgcc
   131941 aatattgctc gccataatat ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta
   132001 atgggtcagg aaaagctata catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc
   132061 gtgcttcagg aagcggcgga caaatctaac ccgctgattg aaaggatgcg tttcctgggg
   132121 atctattcca ataaccttga tgagttctat aaagtccgct tcgctgaact gaagcgacgc
   132181 atcattatta gcgaagaaca aggctccaac gctcattccc gccatttact gggcaaaatt
   132241 cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga gctattgctg
   132301 gagatggcgc gcaaccagat cttcctgatt aatgaacgcc agctctccgt caatcaacaa
   132361 aactggctgc gtcattattt taagcagtat ctgcgtcagc acattacgcc gattttaatc
   132421 aatcctgaca ctgacttagt gcagttcctg aaagatgatt acacctatct ggcggtggaa
   132481 attatccgta gcgatagcat ccgttacgcg ctgctggaga tcccatcaga taaagtgccg
   132541 cgctttgtga atttaccgcc agaagcgccg cgtcgacgca agccgatgat tcttctggat
   132601 aacattctgc gttactgcct tgatgatatt ttcaaaggct tctttgatta tgacgcgctg
   132661 aatgcctatt caatgaagat gacccgcgat gccgaatacg atttagtgca tgagatggaa
   132721 gccagcctga tggagttgat gtcttccagt ctcaagcagc gtttaactgc tgagccggtg
   132781 cgttttgttt atcagcgcga tatgcccaat gcgctggttg aagtgttacg cgaaaaactg
   132841 actatttccc gctacgactc catcgtcccc ggcggtcgtt atcataattt taaagacttt
   132901 attaatttcc ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc gcgtttacgc
   132961 catatttggt ttgataaagc ccagttccgc aatggttttg atgccattcg cgaacgcgat
   133021 gtgttgctct attatcctta tcacaccttt gagcatgtgc tggaactgct gcgtcaggct
   133081 tcgttcgacc cgagcgtact ggcgattaaa attaacattt accgcgtggc gaaagattca
   133141 cgcatcatcg actcgatgat ccacgccgca cataacggta agaaagtcac tgtggtggtt
   133201 gagttacagg cgcgtttcga cgaagaagcc aacattcact gggcgaagcg cctgaccgaa
   133261 gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg
   133321 atttcacgta aagaaaacgg tgaagtggtc cgttacgcac acatcgggac cgggaacttt
   133381 aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc tgaccgcaga tgcgcgtatc
   133441 accaacgaag tacggcgggt atttaacttt attgaaaacc cataccgccc ggtgacattt
   133501 gattatttaa tggtgtcacc gcaaaactct cgccgtctgt tatatgaaat ggtagaccgc
   133561 gaaatcgcca acgcgcagca agggctgccc agtggtatca ccctgaagct aaataacctt
   133621 gtcgataaag gcctggttga tcgtctgtat gcggcctcca gctccggcgt accggttaat
   133681 ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg aaggcattag cgacaacatt
   133741 cgtgccatca gtattgttga ccgttacctt gaacatgacc gggtttatat ttttgaaaat
   133801 ggcggcgata aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa tattgattat
   133861 cgtattgaag tggcgacacc gctgctcgat ccgcgcctga agcagcgggt gctggacatc
   133921 atcgacatat tgttcagcga tacggtcaaa gcacgttata tcgataaaga actcagtaat
   133981 cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat ttacgactac
   134041 atcaaatcac tcgaacaacc tgaataaccc tatgccaata cacgataaat cccctcgtcc
   134101 gcaggagttt gctgcggtcg atcttggttc aaacagtttt cacatggtca tagcccgtgt
   134161 ggtagatggt gccatgcaga ttattggccg cctgaaacag cgggtgcatc tggcggacgg
   134221 cctggggcca gataatatgt tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc
   134281 gctgtttgcc gaacggctac aagggttttc tcctgccagc gtctgtatag tcggcaccca
   134341 tacgctgcgt caggcgctga acgccactga ctttctgaaa cgcgcggaaa aggtcattcc
   134401 ctacccgatt gaaattattt ccggtaatga agaagcccgt ctgattttta tgggcgtgga
   134461 acatacccaa ccggaaaaag gtcgcaaact ggttattgat attggcggcg gatctacgga
   134521 actggtgatt ggtgaaaatt tcgaacctat tctcgttgaa agccgccgga tgggttgtgt
   134581 cagctttgcc cagctttatt ttcctggcgg ggtcatcaat aaagagaatt ttcagcgcgc
   134641 tcgcatggcg gcagcacaaa aactggaaac tttaacctgg caattccgta ttcagggctg
   134701 gaacgttgca atgggcgctt ccggtaccat aaaagccgcc catgaagtgt taatggaaat
   134761 gggcgagaaa gacgggataa ttaccccgga acgtctggaa aaactggtaa aagaaatttt
   134821 acgtcaccgt aatttcgcat cgctgagttt accgggtctt tccgaagagc ggaaaacagt
   134881 cttcgttcct ggactggcga ttttatgtgg tgtgtttgat gctttagcca tccgtgaact
   134941 gcgcctttct gacggggcgt tacgcgaagg cgtactgtat gaaatggaag gacgtttccg
   135001 tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc aaccagtatc acatagacag
   135061 cgaacaggcc cgacgagtgc tggataccac tatgcaaatg tacgaacagt ggcgggaaca
   135121 gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg cgatgggccg ccatgctgca
   135181 tgaggtcggg ttgaatatca accacagcgg tttgcatcgc cactccgctt atattctgca
   135241 aaacagtgac ttgccgggtt ttaatcagga acagcagctg atgatggcga cactggtgcg
   135301 ctatcaccgt aaagcgatta agctcgacga tctgccgcgc tttaccttgt ttaagaagaa
   135361 acagttcctg ccactgatac agctattgcg ccttggcgta ttactaaaca atcaacgtca
   135421 ggcaaccacc acaccgccaa cattgacact gattactgat gacagtcact ggacactgcg
   135481 tttcccgcat gactggttta gtcagaatgc gctggtactg cttgatctgg aaaaggagca
   135541 agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa gtaccccaga
   135601 aatcgccgct taatgctttg cgggccgacg agaaatgtcg gcccgcatta ttcaggcact
   135661 ttcgcgaatg ggttcgattt cattcagcgt atcaattaac ggttgcggct taccaataag
   135721 ataaccctgc atataatcga tccccaaaga gagcaccgcc tcgcggatct cttcgttttc
   135781 aacgtactct gccactaccc gcattttctt cattcgcgcc agatggcaaa tcgacgccac
   135841 aatctgataa tccagactat ttgacacaat attgcggata aagctgccgt caattttaag
   135901 cagatcggca ttcacatttt taagccgtgc atagctggcg tagccggtgc cgaaatcatc
   135961 aatcgcaatc tggcagccta attcctgaag atgctgcaag gtgatttgcg cctgcttaac
   136021 attggtcaga gcattacttt cggtgacttc aaaaataagt tgccacgctt caatctgata
   136081 tttagccagc aactgactga cttcaacagg aaaacgagcc tgacataccg aggttggaga
   136141 cagattaata gcaaaacggt gagcgggcat cttcgctctg ttttcagcca taaattgcag
   136201 cgtatgctca atgacccaca tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg
   136261 caagaagcta ccggggccga tcagttcatc attctcacct ttcatgcgca gaagaatttc
   136321 atggtaaaca tcaccacgca taccggtaat cggctgggcc atcaggaaaa aatggttgtg
   136381 ttccagcgcc tgctgtagtc gattcatcat cgcgacttta tctttcaatt cgcgttgcaa
   136441 atacattgcc ccgcaacgct gcatattttc cggggcgttg gtcacgatgg aaagttcggc
   136501 gaccgtattt agctctccca gcagcaggta gatatgattc actggcgagc gcacatagca
   136561 gtaactgacg ccaatctgcg gttgcatcgg catcccatcc caaaagaaac gaaattgctt
   136621 gagatgacta tccagtgcgg taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc
   136681 gagatcgtta cccgaaagct gataaacatc ttcacccggt tccagcaagg gtgacagcca
   136741 gtgagaaagt ttttgcttgt
//
LOCUS       DAJTNP010000002        94921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTNP010000002 DAJTNP010000000
VERSION     DAJTNP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1020)
                     /gene="icd"
                     /locus_tag="OO160_000132"
     CDS             complement(<1..1020)
                     /gene="icd"
                     /locus_tag="OO160_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5522409.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHG"
     gene            1192..1845
                     /gene="rluE"
                     /locus_tag="OO160_000133"
     CDS             1192..1845
                     /gene="rluE"
                     /locus_tag="OO160_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5522410.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            1855..2316
                     /gene="nudJ"
                     /locus_tag="OO160_000134"
     CDS             1855..2316
                     /gene="nudJ"
                     /locus_tag="OO160_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5522411.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2370..3476
                     /gene="mnmA"
                     /locus_tag="OO160_000135"
     CDS             2370..3476
                     /gene="mnmA"
                     /locus_tag="OO160_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5522412.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3512..4153
                     /gene="hflD"
                     /locus_tag="OO160_000136"
     CDS             3512..4153
                     /gene="hflD"
                     /locus_tag="OO160_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5522413.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4157..5527
                     /gene="purB"
                     /locus_tag="OO160_000137"
     CDS             4157..5527
                     /gene="purB"
                     /locus_tag="OO160_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5522414.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5696..6367
                     /gene="phoP"
                     /locus_tag="OO160_000138"
     CDS             5696..6367
                     /gene="phoP"
                     /locus_tag="OO160_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5522415.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6367..7827
                     /gene="phoQ"
                     /locus_tag="OO160_000139"
     CDS             6367..7827
                     /gene="phoQ"
                     /locus_tag="OO160_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5522416.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8624..8905)
                     /locus_tag="OO160_000140"
     CDS             complement(8624..8905)
                     /locus_tag="OO160_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522417.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(8919..10580)
                     /locus_tag="OO160_000141"
     CDS             complement(8919..10580)
                     /locus_tag="OO160_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5522418.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10564..10890)
                     /locus_tag="OO160_000142"
     CDS             complement(10564..10890)
                     /locus_tag="OO160_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5522419.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLEQYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(10946..11128)
                     /locus_tag="OO160_000143"
     CDS             complement(10946..11128)
                     /locus_tag="OO160_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522420.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11112..11552)
                     /locus_tag="OO160_000144"
     CDS             complement(11112..11552)
                     /locus_tag="OO160_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5522421.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11552..11848)
                     /locus_tag="OO160_000145"
     CDS             complement(11552..11848)
                     /locus_tag="OO160_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5522422.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(11845..12183)
                     /locus_tag="OO160_000146"
     CDS             complement(11845..12183)
                     /locus_tag="OO160_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5522423.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12180..13391)
                     /locus_tag="OO160_000147"
     CDS             complement(12180..13391)
                     /locus_tag="OO160_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5522424.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13393..13965)
                     /locus_tag="OO160_000148"
     CDS             complement(13393..13965)
                     /locus_tag="OO160_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5522425.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14005..15162)
                     /locus_tag="OO160_000149"
                     /pseudo
     CDS             complement(14005..15162)
                     /locus_tag="OO160_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15463..15687)
                     /locus_tag="OO160_000150"
     CDS             complement(15463..15687)
                     /locus_tag="OO160_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522426.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(15829..15990)
                     /locus_tag="OO160_000151"
     CDS             complement(15829..15990)
                     /locus_tag="OO160_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5522427.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16000..16305)
                     /locus_tag="OO160_000152"
     CDS             complement(16000..16305)
                     /locus_tag="OO160_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522428.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16302..16580)
                     /locus_tag="OO160_000153"
     CDS             complement(16302..16580)
                     /locus_tag="OO160_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522429.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16869..18617)
                     /locus_tag="OO160_000154"
     CDS             complement(16869..18617)
                     /locus_tag="OO160_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5522430.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18614..18913)
                     /locus_tag="OO160_000155"
     CDS             complement(18614..18913)
                     /locus_tag="OO160_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5522431.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18919..19152)
                     /locus_tag="OO160_000156"
     CDS             complement(18919..19152)
                     /locus_tag="OO160_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522432.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19154..19374)
                     /locus_tag="OO160_000157"
                     /pseudo
     CDS             complement(19154..19374)
                     /locus_tag="OO160_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19367..19588)
                     /locus_tag="OO160_000158"
     CDS             complement(19367..19588)
                     /locus_tag="OO160_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522433.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19578..19778)
                     /locus_tag="OO160_000159"
     CDS             complement(19578..19778)
                     /locus_tag="OO160_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5522434.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19908..20087
                     /locus_tag="OO160_000160"
     CDS             19908..20087
                     /locus_tag="OO160_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522435.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20212..20541
                     /locus_tag="OO160_000161"
     CDS             20212..20541
                     /locus_tag="OO160_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522436.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20591..20779)
                     /locus_tag="OO160_000162"
     CDS             complement(20591..20779)
                     /locus_tag="OO160_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5522437.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21154..22383)
                     /locus_tag="OO160_000163"
     CDS             complement(21154..22383)
                     /locus_tag="OO160_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5522438.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22632..23753
                     /gene="roxA"
                     /locus_tag="OO160_000164"
                     /gene_synonym="ycfD"
     CDS             22632..23753
                     /gene="roxA"
                     /locus_tag="OO160_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5522439.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23802..25028)
                     /gene="pepT"
                     /locus_tag="OO160_000165"
     CDS             complement(23802..25028)
                     /gene="pepT"
                     /locus_tag="OO160_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5522440.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25278..26414
                     /gene="potA"
                     /locus_tag="OO160_000166"
     CDS             25278..26414
                     /gene="potA"
                     /locus_tag="OO160_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5522441.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26398..27255
                     /gene="potB"
                     /locus_tag="OO160_000167"
     CDS             26398..27255
                     /gene="potB"
                     /locus_tag="OO160_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5522442.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27252..28046
                     /gene="potC"
                     /locus_tag="OO160_000168"
     CDS             27252..28046
                     /gene="potC"
                     /locus_tag="OO160_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5522443.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28043..29089
                     /gene="potD"
                     /locus_tag="OO160_000169"
     CDS             28043..29089
                     /gene="potD"
                     /locus_tag="OO160_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5522444.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29245..30066)
                     /gene="cobB"
                     /locus_tag="OO160_000170"
     CDS             complement(29245..30066)
                     /gene="cobB"
                     /locus_tag="OO160_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5522445.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30082..30993)
                     /gene="nagK"
                     /locus_tag="OO160_000171"
     CDS             complement(30082..30993)
                     /gene="nagK"
                     /locus_tag="OO160_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5522446.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31022..32266)
                     /gene="lolE"
                     /locus_tag="OO160_000172"
     CDS             complement(31022..32266)
                     /gene="lolE"
                     /locus_tag="OO160_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5522447.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32266..32967)
                     /gene="lolD"
                     /locus_tag="OO160_000173"
     CDS             complement(32266..32967)
                     /gene="lolD"
                     /locus_tag="OO160_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5522448.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32960..34159)
                     /gene="lolC"
                     /locus_tag="OO160_000174"
     CDS             complement(32960..34159)
                     /gene="lolC"
                     /locus_tag="OO160_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5522449.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34421..35494
                     /locus_tag="OO160_000175"
     CDS             34421..35494
                     /locus_tag="OO160_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5522450.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35622..39068
                     /gene="mfd"
                     /locus_tag="OO160_000176"
     CDS             35622..39068
                     /gene="mfd"
                     /locus_tag="OO160_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5522451.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39211..40173
                     /gene="ldtC"
                     /locus_tag="OO160_000177"
     CDS             39211..40173
                     /gene="ldtC"
                     /locus_tag="OO160_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5522452.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40256..40468)
                     /locus_tag="OO160_000178"
     CDS             complement(40256..40468)
                     /locus_tag="OO160_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5522453.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40753..41385
                     /gene="comR"
                     /locus_tag="OO160_000179"
     CDS             40753..41385
                     /gene="comR"
                     /locus_tag="OO160_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5522454.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41447..41986)
                     /locus_tag="OO160_000180"
     CDS             complement(41447..41986)
                     /locus_tag="OO160_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5522455.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42213..43517)
                     /gene="ndh"
                     /locus_tag="OO160_000181"
     CDS             complement(42213..43517)
                     /gene="ndh"
                     /locus_tag="OO160_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5522456.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43925..44467)
                     /locus_tag="OO160_000182"
     CDS             complement(43925..44467)
                     /locus_tag="OO160_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5522457.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44490..45515)
                     /gene="nagZ"
                     /locus_tag="OO160_000183"
     CDS             complement(44490..45515)
                     /gene="nagZ"
                     /locus_tag="OO160_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5522458.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45526..46350)
                     /gene="thiK"
                     /locus_tag="OO160_000184"
     CDS             complement(45526..46350)
                     /gene="thiK"
                     /locus_tag="OO160_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5522459.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46331..46972)
                     /gene="lpoB"
                     /locus_tag="OO160_000185"
     CDS             complement(46331..46972)
                     /gene="lpoB"
                     /locus_tag="OO160_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5522460.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46987..47364)
                     /locus_tag="OO160_000186"
     CDS             complement(46987..47364)
                     /locus_tag="OO160_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5522461.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47367..47726)
                     /gene="hinT"
                     /locus_tag="OO160_000187"
     CDS             complement(47367..47726)
                     /gene="hinT"
                     /locus_tag="OO160_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5522462.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            47857..48038
                     /locus_tag="OO160_000188"
                     /pseudo
     CDS             47857..48038
                     /locus_tag="OO160_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            48071..50256
                     /gene="fhuE"
                     /locus_tag="OO160_000189"
                     /pseudo
     CDS             48071..50256
                     /gene="fhuE"
                     /locus_tag="OO160_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50316..51749)
                     /gene="ptsG"
                     /locus_tag="OO160_000190"
     CDS             complement(50316..51749)
                     /gene="ptsG"
                     /locus_tag="OO160_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5522463.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52044..52841)
                     /locus_tag="OO160_000191"
     CDS             complement(52044..52841)
                     /locus_tag="OO160_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5522464.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52852..53856)
                     /gene="holB"
                     /locus_tag="OO160_000192"
     CDS             complement(52852..53856)
                     /gene="holB"
                     /locus_tag="OO160_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5522465.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53853..54494)
                     /locus_tag="OO160_000193"
     CDS             complement(53853..54494)
                     /locus_tag="OO160_000193"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5522466.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54484..55506)
                     /gene="yceG"
                     /locus_tag="OO160_000194"
     CDS             complement(54484..55506)
                     /gene="yceG"
                     /locus_tag="OO160_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5522467.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55509..56318)
                     /gene="pabC"
                     /locus_tag="OO160_000195"
     CDS             complement(55509..56318)
                     /gene="pabC"
                     /locus_tag="OO160_000195"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5522468.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56438..57679)
                     /gene="fabF"
                     /locus_tag="OO160_000196"
     CDS             complement(56438..57679)
                     /gene="fabF"
                     /locus_tag="OO160_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5522469.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57766..58002)
                     /gene="acpP"
                     /locus_tag="OO160_000197"
     CDS             complement(57766..58002)
                     /gene="acpP"
                     /locus_tag="OO160_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5522470.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58213..58947)
                     /gene="fabG"
                     /locus_tag="OO160_000198"
     CDS             complement(58213..58947)
                     /gene="fabG"
                     /locus_tag="OO160_000198"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5522471.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58960..59889)
                     /gene="fabD"
                     /locus_tag="OO160_000199"
     CDS             complement(58960..59889)
                     /gene="fabD"
                     /locus_tag="OO160_000199"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5522472.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59905..60858)
                     /gene="fabH"
                     /locus_tag="OO160_000200"
     CDS             complement(59905..60858)
                     /gene="fabH"
                     /locus_tag="OO160_000200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5522473.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60926..61996)
                     /gene="plsX"
                     /locus_tag="OO160_000201"
     CDS             complement(60926..61996)
                     /gene="plsX"
                     /locus_tag="OO160_000201"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5522474.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62077..62250)
                     /gene="rpmF"
                     /locus_tag="OO160_000202"
     CDS             complement(62077..62250)
                     /gene="rpmF"
                     /locus_tag="OO160_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5522475.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62302..62823)
                     /gene="yceD"
                     /locus_tag="OO160_000203"
     CDS             complement(62302..62823)
                     /gene="yceD"
                     /locus_tag="OO160_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5522476.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63022..63606
                     /gene="yceF"
                     /locus_tag="OO160_000204"
     CDS             63022..63606
                     /gene="yceF"
                     /locus_tag="OO160_000204"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5522477.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63718..64677)
                     /gene="rluC"
                     /locus_tag="OO160_000205"
     CDS             complement(63718..64677)
                     /gene="rluC"
                     /locus_tag="OO160_000205"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5522478.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65250..68441
                     /gene="rne"
                     /locus_tag="OO160_000206"
     CDS             65250..68441
                     /gene="rne"
                     /locus_tag="OO160_000206"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5522479.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68638..69580)
                     /gene="flgL"
                     /locus_tag="OO160_000207"
                     /pseudo
     CDS             complement(68638..69580)
                     /gene="flgL"
                     /locus_tag="OO160_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69592..71234)
                     /gene="flgK"
                     /locus_tag="OO160_000208"
                     /pseudo
     CDS             complement(69592..71234)
                     /gene="flgK"
                     /locus_tag="OO160_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71300..72241)
                     /gene="flgJ"
                     /locus_tag="OO160_000209"
     CDS             complement(71300..72241)
                     /gene="flgJ"
                     /locus_tag="OO160_000209"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5522480.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72241..73338)
                     /gene="flgI"
                     /locus_tag="OO160_000210"
     CDS             complement(72241..73338)
                     /gene="flgI"
                     /locus_tag="OO160_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5522481.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73350..74048)
                     /gene="flgH"
                     /locus_tag="OO160_000211"
     CDS             complement(73350..74048)
                     /gene="flgH"
                     /locus_tag="OO160_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5522482.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74101..74883)
                     /gene="flgG"
                     /locus_tag="OO160_000212"
     CDS             complement(74101..74883)
                     /gene="flgG"
                     /locus_tag="OO160_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5522483.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75021..75772)
                     /gene="flgF"
                     /locus_tag="OO160_000213"
                     /pseudo
     CDS             complement(75021..75772)
                     /gene="flgF"
                     /locus_tag="OO160_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75792..77000)
                     /gene="flgE"
                     /locus_tag="OO160_000214"
     CDS             complement(75792..77000)
                     /gene="flgE"
                     /locus_tag="OO160_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5522484.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77025..77720)
                     /gene="flgD"
                     /locus_tag="OO160_000215"
     CDS             complement(77025..77720)
                     /gene="flgD"
                     /locus_tag="OO160_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5522485.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77732..78135)
                     /gene="flgC"
                     /locus_tag="OO160_000216"
                     /pseudo
     CDS             complement(77732..78135)
                     /gene="flgC"
                     /locus_tag="OO160_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78139..78555)
                     /gene="flgB"
                     /locus_tag="OO160_000217"
     CDS             complement(78139..78555)
                     /gene="flgB"
                     /locus_tag="OO160_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5522486.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78710..79369
                     /gene="flgA"
                     /locus_tag="OO160_000218"
     CDS             78710..79369
                     /gene="flgA"
                     /locus_tag="OO160_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5522487.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79445..79738
                     /gene="flgM"
                     /locus_tag="OO160_000219"
     CDS             79445..79738
                     /gene="flgM"
                     /locus_tag="OO160_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5522488.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79743..80159
                     /gene="flgN"
                     /locus_tag="OO160_000220"
     CDS             79743..80159
                     /gene="flgN"
                     /locus_tag="OO160_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5522489.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80199..81734)
                     /gene="murJ"
                     /locus_tag="OO160_000221"
     CDS             complement(80199..81734)
                     /gene="murJ"
                     /locus_tag="OO160_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5522490.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81934..82767)
                     /locus_tag="OO160_000222"
     CDS             complement(81934..82767)
                     /locus_tag="OO160_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5522491.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82769..83416)
                     /locus_tag="OO160_000223"
     CDS             complement(82769..83416)
                     /locus_tag="OO160_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5522492.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83427..84011)
                     /gene="rimJ"
                     /locus_tag="OO160_000224"
     CDS             complement(83427..84011)
                     /gene="rimJ"
                     /locus_tag="OO160_000224"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5522493.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84247..85455
                     /gene="mdtH"
                     /locus_tag="OO160_000225"
     CDS             84247..85455
                     /gene="mdtH"
                     /locus_tag="OO160_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5522494.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85519..86166
                     /gene="grxB"
                     /locus_tag="OO160_000226"
     CDS             85519..86166
                     /gene="grxB"
                     /locus_tag="OO160_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5522495.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86300..86860
                     /locus_tag="OO160_000227"
     CDS             86300..86860
                     /locus_tag="OO160_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5522496.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86966..88012
                     /gene="pyrC"
                     /locus_tag="OO160_000228"
     CDS             86966..88012
                     /gene="pyrC"
                     /locus_tag="OO160_000228"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5522497.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88086..88331
                     /gene="dinI"
                     /locus_tag="OO160_000229"
     CDS             88086..88331
                     /gene="dinI"
                     /locus_tag="OO160_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5522498.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88621..88875
                     /gene="bssS"
                     /locus_tag="OO160_000230"
     CDS             88621..88875
                     /gene="bssS"
                     /locus_tag="OO160_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5522499.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88990..90108
                     /gene="solA"
                     /locus_tag="OO160_000231"
     CDS             88990..90108
                     /gene="solA"
                     /locus_tag="OO160_000231"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5522500.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90156..90269
                     /locus_tag="OO160_000232"
     CDS             90156..90269
                     /locus_tag="OO160_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5522501.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90530..91096
                     /locus_tag="OO160_000233"
     CDS             90530..91096
                     /locus_tag="OO160_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5522502.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91100..91675
                     /locus_tag="OO160_000234"
     CDS             91100..91675
                     /locus_tag="OO160_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5522503.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91717..92769)
                     /locus_tag="OO160_000235"
     CDS             complement(91717..92769)
                     /locus_tag="OO160_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5522504.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92994..93914
                     /gene="lpxL"
                     /locus_tag="OO160_000236"
     CDS             92994..93914
                     /gene="lpxL"
                     /locus_tag="OO160_000236"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5522505.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94086..94283
                     /locus_tag="OO160_000237"
     CDS             94086..94283
                     /locus_tag="OO160_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5522506.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94307..>94921
                     /locus_tag="OO160_000238"
     CDS             94307..>94921
                     /locus_tag="OO160_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5522507.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQ"
BASE COUNT        22813 a        25176 c        23440 g        23492 t
ORIGIN      
        1 accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg tttgctccag gggcgatacc
       61 gataccgcca acctgcgctg ccagagcgtc agaaatgtag tcaccgttca ggttcataca
      121 ggcgataaca tcatattcag ccggacgcag caggatctgt tgcaggaatg catcagcaat
      181 cacgtcttta atgacgatct ctttgccggt attcgggttt ttaactttca gccacgggcc
      241 gccgtcgatc agttcaccgc caaattcttc acgcgccagc tggtagcccc agtctttaaa
      301 cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc agggtcacag agtcacgatc
      361 gttagcaatt gcgtattcga tcgctgcacg aaccagacgt ttggtgcctt cttcagaaca
      421 cggcttaata ccgataccgc aatgttccgg gaagcgaatt ttcttcacgc ccatctcttc
      481 acgcaggaat ttaatcactt tctcggcgtc ggcagagtca gctttccatt cgatacccgc
      541 ataaatgtct tccgagtttt cacggaagat aaccatatcg gtcagttcag ggtgtttaac
      601 cgggcttgga gtgccctgat agtaacgtac cggacgcagg cagatgtaga gatccagttc
      661 ctgacgcagg gcaacgttga gagaacgaat accgccacca accggagtgg tcagcgggcc
      721 tttaatggca acgcgatatt cacgaatcag atcaagggtt tcagcaggca gccagacatc
      781 ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt tccatccagg agattttacg
      841 ctcgccttta taggctttct cgactgcagc gtcgaccact ttcagcatgg ctggggttac
      901 atctacaccg attccatcac cttcaatgta agggataatc ggattttcag gaacgttgag
      961 tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga acaactactt tactttccat
     1021 tcacctctcc ttcgagcgct actggtttgc tcgtctgcca ccacgttgca tatgcgtttg
     1081 cgtcctgcga tacggatgct ttagagcaat tttttgttaa tgatttgtaa ttgacttgtc
     1141 aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt attaggctat aatgcggcaa
     1201 ttcataatct ctgaaaatac catgcaaaaa acttctttta gaaatcacca ggttaagcga
     1261 ttcagctcgc aacgttctac caggcgtaaa catgaaaacc agcccacgcg tgtgatcctc
     1321 ttcaataaac cctacgatgt tcttccgcag ttcaccgatg aaaccggacg caaaacatta
     1381 aaagaattca tcccggttca gggcgtttat gcagcaggtc gccttgaccg cgatagcgaa
     1441 gggttgctgg tgctgaccaa taacggcgcg ttgcaggcgc gtttaaccca gccgggtaaa
     1501 cgcaccggaa aaatctatta tgtgcaggtg gaaggtattc ccacacaaga cgcacttgaa
     1561 gccttgcgca atggcgtaac cttaaatgat ggccctaccc tgcccgcagg cgcggagctg
     1621 gttgacgaac ccgcgtggtt atggccgcgg aatccaccaa ttcgtgaacg caaaagtatt
     1681 cccaccagct ggctgaagat caccttatat gaaggacgta atcgccaggt gcgccgcatg
     1741 accgcccatg ttggtttccc cacgctgcga ctgattcgct atgcgatggg agattactct
     1801 ttggataatc ttgccaatgg cgaatggcga gaagtgacag attaaggatg caaaatgttt
     1861 aaaccgcacg ttaccgttgc ttgcgtggtg cacgcagaag gcaaattttt agtcgttgaa
     1921 gagacgatta atggtaaagc gttatggaac caacctgccg ggcatctgga agccgatgaa
     1981 accttagtgg aagccgccgc ccgtgaactg tgggaagaaa ccggcatcag cgcgcagccg
     2041 caacacttta ttcgtatgca tcagtggatt gcgccagata aaacgccgtt tttgcgtttc
     2101 ctctttgcca ttgagcttga gcaaatatgc ccgacccagc ctcatgacag cgatatcgac
     2161 tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt caaatcttcg ttcgccgctg
     2221 gtggcggaaa gtattcgttg ttatcaaagc gggcaacgtt atccgctgga gatgattggc
     2281 gattttaact ggccttttac aaagggtgtc atctaaaagg ctgcgtgata gaatacgccg
     2341 ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac cgcaaaaaaa gtaatcgtcg
     2401 gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct gttgcaacaa cagggatatc
     2461 aggtcgaagg cctgtttatg aagaactggg aagaagacga cggtgaggaa tattgcacag
     2521 cagcagcgga tctggctgat gcccaggcgg tctgcgacaa gctcggcatt gaactgcaca
     2581 ccgttaactt tgctgccgag tactgggaca acgtcttcga actgttcctt gccgaatata
     2641 aagccggtcg cacgccgaat ccggatattc tgtgcaacaa ggagatcaaa tttaaagcct
     2701 tcctcgaatt tgccgccgaa gatttaggtg ccgattatat cgctaccggt cattacgtac
     2761 gtcgggccga tgtcgatggc aagagccgcc tgctgcgtgg tctggacagc aataaagacc
     2821 agagctactt cctttatacg ctcagccatg agcagattgc gcaaagcctg ttcccggtag
     2881 gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga tcttggtctg gtcaccgcga
     2941 agaaaaaaga ctctaccggc atttgcttca ttggcgaacg taaattccgc gagttcctgg
     3001 gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt cgatggcgat gaaattggtg
     3061 agcaccaggg gctgatgtat cacactctcg gtcagcgtaa aggtctgggt atcggtggca
     3121 ccaaagaagg taccgaagaa ccgtggtatg tggtggacaa agacgtcgaa aacaacattc
     3181 tggttgtcgc tcagggccat gaacacccgc ggctgatgtc tgtcgggttg attgcccagc
     3241 agctgcactg ggtcgatcgc gaaccattca ccggcactat gcgttgcacg gtaaaaaccc
     3301 gctatcgcca gaccgacatt ccgtgcaccg tcaaggcgct ggacgacgat cgcattaaag
     3361 tgattttcga tgagccggtt gccgccgtga cgccgggcca gtctgccgtc ttctataacg
     3421 gtgaagtgtg cctcggtggc ggtattattg agcagcgtct gccgctgccg gtctgattat
     3481 tatctttact taaaacaagg aagcagtgaa cgtggcaaag aattactatg acatcaccct
     3541 cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa caactcgctc accaggggca
     3601 ttgtgatgcc gatgcgctac acgtctcact caacagtatt attgatatga accccagctc
     3661 gacgctggcg gtttttggcg gtagcgaagc caacctgcgc gtcgggctgg aaaccctgct
     3721 cggcgtgctc aatgccagca gttgccaggg cttaaacgcc gaattaaccc gctacacact
     3781 cagcttgatg gtgcttgagc gcaaactctc ctcagcgaaa ggcgcgctcg acactctggg
     3841 caaccggatc aacggcctgc aacgccagct cgaacacttc gatttacagt ccgaaacgct
     3901 gatgagcgcg atggctgcta tctatgttga tgtgatcagc ccgcttggcc cgcgcattca
     3961 ggtcaccggt tcccctgctg tactgcaaag cccacaagtg caggcgaaag ttcgcgcaac
     4021 cctgctggca ggcattcgcg ccgccgtgct ctggcaccag gtcggcggcg gacgtctaca
     4081 actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa caaattcttg ctcatttaac
     4141 cccggagttg tgatctatgg aattatcctc actgaccgcc gtttcccctg tcgatggacg
     4201 ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc gaatatggtt tgctgaaatt
     4261 ccgtgtacaa gttgaagtac gttggctgca aaaactggcc gcgcacgcag cgatcaagga
     4321 agttcctgct tttgctgccg acgcaatcgg ttaccttgat gcaattgtcg ccaatttcag
     4381 cgaagaagat gccgcacgca tcaaaaccat cgagcgtacc actaaccacg acgttaaagc
     4441 ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg gaactgcacg cggtttctga
     4501 attcatccac tttgcctgta cttcggaaga tatcaataac ctctcccacg cattaatgct
     4561 gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt caactgattg atggcattaa
     4621 agatctcgcc gttcagtacc gcgatatccc gctgctgtct cgtacccacg gtcagccagc
     4681 cacgccgtca accatcggta aagagatggc aaacgtcgcc taccgtatgg agcgccagca
     4741 ccgccagctt aaccaggtgg agatcctcgg caaaatcaac ggcgcggtcg gtaactataa
     4801 cgcccacatc gccgcttacc cggaagttga ctggcatcag ttcagcgaag agttcgtcac
     4861 ctcgctgggt attcaatgga acccgtacac tacccagatt gaaccgcacg actacattgc
     4921 cgaactgttt gattgcgttg cgcgcttcaa caccattctg atcgactttg accgtgacgt
     4981 ctggggttat atcgccctta accacttcaa acagaaaacc attgctggtg agattggttc
     5041 ttccaccatg ccgcataaag ttaacccgat cgacttcgaa aactccgaag ggaatctggg
     5101 cctttccaac gcggtattgc agcatctggc aagcaaactg ccggtttccc gctggcagcg
     5161 tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg ggtatcggtt atgcgctgat
     5221 tgcgtatcaa tccaccctga aaggcgtgag caaactggaa gtgaaccgtg accatctgct
     5281 ggatgagctg gatcacaact gggaagtgct ggcagaacca atccagacag ttatgcgtcg
     5341 ctatggcatc gaaaaaccgt acgagaagct gaaagagctt actcgcggta agcgcgttga
     5401 cgccgaaggc atgaagcagt ttatcgatgg tctggcgttg ccagaagaag agaaagcccg
     5461 cctgaaagcg atgacgccgg ctaactatat tggtcgagct atcacgatgg ttgatgagct
     5521 gaaataagcc tcaaatcagt gccggatggc gatgctgtcc ggcctgctta ttaagattat
     5581 ccactttttc ttttttcact ttacctcccc tccccgctgg tttatttaat gtttaccccc
     5641 ataaacacat aatcgcgtta cactatttta ataattaaga cagggagaaa taaaaatgcg
     5701 cgtactggtt gttgaagaca atgcgttgtt acgtcaccac cttaaagttc agattcagga
     5761 tgctggtcat caggtcgatg acgcagaaga tgccaaagaa gccgattatt atctcaatga
     5821 acatttaccg gatattgcga ttgtcgatct cggattgcct gacgaggacg gtctgtcact
     5881 gattcgccgc tggcgtagca acgatgtttc actgccgatt ctggtattaa ccgcccgtga
     5941 aagctggcag gacaaagtcg aagtattaag tgccggtgct gatgattacg tgactaaacc
     6001 gtttcatatt gaagaggtga tggcgcgaat gcaggcatta atgcggcgta atagcggtct
     6061 ggcttcacag gtcatttcgc tccccccgtt tcaggttgat ctctctcgcc gtgaattatc
     6121 tattaatgac gaagtgatca aactgaccgc gttcgaatac accattatgg aaacgttgat
     6181 acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg ctacaactct atccggatgc
     6241 ggagctgcgg gaaagccata ccattgatgt actgatggga cgtctgcgca aaaaaattca
     6301 ggcacaatat ccgcaagaag tcattaccac cgttcgcggc cagggctacc tgttcgaatt
     6361 gcgctgatga aaaaattact gcgtcttttt ttcccgctct cgctgcgggt acgttttctg
     6421 ttggcaacgg cagcggtagt actggtgctt tcgcttgcct acggaatggt cgcgctgatc
     6481 ggttatagcg tcagtttcga taaaactacg tttcggctgt tacgtggcga gagcaatctg
     6541 ttctataccc ttgcgcagtg ggaaaacaat aagttgcatg tcgagttacc cgaaaatatc
     6601 gacaagcaaa gccccaccat gacgctaatt tatgatgaga acgggcagct tttatgggcg
     6661 caacgtgacg tgccctggct gatgaaaatg atccagcctg actggctgaa atcgaatggt
     6721 tttcatgaaa ttgaagcgga tgttaacgat accagcctct tgctgagtgg agatcattcg
     6781 atacagcaac agttgcagga agtgcgggaa gatgatgacg acgcggagat gacccactcg
     6841 gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa aattaaccat tgtggtggtg
     6901 gataccattc cggtggagct aaaaagttcc tatatggtct ggagctggtt tatctatgtg
     6961 ctctcagcca atctgctgtt agtgatcccg ctgctgtggg tcgccgcctg gtggagttta
     7021 cgccccatcg aagccctggc aaaagaagtc cgcgaactgg aagaacataa ccgcgaattg
     7081 ctcaatccag ccacaacgcg agaactgacc agtctggtac gaaacctgaa ccgattgtta
     7141 aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc tcaccgacct gacccatagt
     7201 ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt ctctgcgtag tgaaaagatg
     7261 agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca gccgcatttc acagcaaatt
     7321 ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat tgctcagccg cgagctgcat
     7381 ccggtcgccc cactgctgga caatctcacc tcagcgctga acaaagtgta tcaacgcaaa
     7441 ggggtcaata tctctctcga tatttcgcca gagatcagct ttgtcggtga gcagaacgat
     7501 tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta aatattgcct cgagtttgtc
     7561 gaaatttctg caaggcaaac cgacgagcat ctctatattg tggtcgagga tgatggcccc
     7621 ggtattccat taagcaagcg agaggtcatt ttcgaccgtg gtcaacgggt tgatacttta
     7681 cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa tcaccgagca atatgagggt
     7741 aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga tggaggtgat ttttggtcgc
     7801 cagcattctg cgccgaaaga tgaataaata tgtcctttta ccactacatc aagtcattga
     7861 ttcgatcaaa caattttcat tgaaaaagtg ccaaaaatcg caaattgact acaccattaa
     7921 ctacaccgtt cggtgcactg tatgaaacaa cgtggaacaa atagacacaa gaaatataca
     7981 ggcgggtaat ctttccaggg ggaagcgcac caattcatga ggggcgttaa tgtcgatatg
     8041 gggatcccca taacggggct accggatttt ttttccggtt aacctttaat caggctggtg
     8101 ggttttacat acccttgatc accgtaatga tgatccagat gtgcaccttt tccaccaatg
     8161 gcggaaaagc tccaaccgta acgatgatcg ctatggttgc ctcgatggtc tgcgtggtca
     8221 ttatgaccgt tcagccagtg accttaatca gaatttttcg ctaaaaaatg acgttggcca
     8281 cgtcatccgg aaagcacaaa atcacgtcga tttttaatga acggttaaac cgtctttttg
     8341 tcacggtgtg tcactggttg tcactctccg tcacgattga ggtggtggtc attctgcccg
     8401 accgcgcatc tttgcgctat cgggaatatc aaacagttac ctcgccgact tcctcaaatt
     8461 gaggtttccg aaaatatcag agcgggttag cgattaagct gacgagtaat taaactgcga
     8521 aaatttcgta gtttctgaat atattccggc ctgctggtta acagtcctca gatgtgaggg
     8581 atgtagccag cgcaattttg cgctttgctt taatctcagt gagttaccgc cgtaaccgct
     8641 ccggcttctt ccagtggtac gtgattttct ccttctcccg atacagttca acgcggcgat
     8701 tgtaggccag catttccaga acgcggatcc gtatgtcgcg catatccgca tcattaagct
     8761 ggataccatc acggcgcatc acctcagcaa caacacgcgc ataattttcg gctgtcacgc
     8821 tgtccggctg cgtggtctgt tcgtcagcct gctggctgat tccagagacg cggcggatta
     8881 atcgcagtat ttcggcttct gtcatgctgc tgacctcatt acaccccgct aaattcttcc
     8941 agtttctggc ggtggctgtc gcttatatca aaagcaaaat cctcatgctc tgcctggaat
     9001 gtaccaaacg ccatcagcgc cgctacgctc gggtctatct tgttcggtga ttttttcttg
     9061 ttcggcttga tattggcgtt cgcgtcactc tgcatcacga cgttactcat cgaccaggac
     9121 aacaccggat cgccacgatg cacaatcacc ctgcggttaa caaaaacttc gaacgatttc
     9181 gccgccggac tgaatctgag gtaggtttgc gggaacggct ccacctcaaa accagccccc
     9241 tgtaattgcg tcctgagatg cgtggcgttc cacgtatcaa agcccaccag cctgatatta
     9301 aatttctccg catcctgcat gatgtcatct ctgatccggt cgtaatcaat gcagtcacct
     9361 ggcgttgtgc gtatccagcc cgctttagcc cactggcgat agacagcgcg gtttttattg
     9421 gcggggttct gtagctggaa ctccggcaga taatgacggg aaatcagcat aatggtttta
     9481 ccgaccggaa aggcatagca tacgctggaa atatcgctgg ttgatgataa gtccagcccc
     9541 gcgtaacact cctgaccgtg taaatcttcc tccgtgaacg ttccggcaca ctcagcccat
     9601 gcaccattac ccatccacgg ggtagtcccc tgacaccaga tattaaatcg ctttgtcatc
     9661 atctccaccc actgcgacgg aataccccgc gctttctgga tggttgaggc cagtttttca
     9721 cgatccacgg aaacatcaat attagggttc gcctttatcc acatcgccgg atcatcaacc
     9781 tcgttttcgt cgtccagttc gtaaatcagt acaaaaattg aatcgttaac ctcttcgccg
     9841 tccaggatct ggcagcaata atcatagtgc tgtttacagg ctgaaacgac gttactgccc
     9901 gatgtggtaa tggcaaataa cagcccttcg ggacgtgcgc ccatccccag ctcaagcgcg
     9961 gaataaacgc cgttatcggg gtgtaggtgg tattcgtcca cgatggaaag actggggttt
    10021 gtcccctcga tggttgccgc ttttgctgcc agcggcttta acaggctgtt gcttttcggg
    10081 tgtatcacct tatgcgcctg aatattcacc cgcctgcgta acggtcggga taaaaggcac
    10141 atctgacgcg cattatcaaa cacgatccgc gcctggtcac ggctcaccgc tgcggtgtag
    10201 atatcctgct gaccgttctc cataatcaga aaccagttag ccagaatcgc ggcggtcgtg
    10261 gatttcgcat ttttgcgcgg cacttcgata aaggcgctcg tgtatttgcg ccgtccggtg
    10321 gccttaacct taaagccgag gatgcacgca aaggcgaact gctgccacgg ctccagctca
    10381 atgggtctgc cacgcatcgg tccttttacg tgcgggcaca ccctggaaaa ggcaataaac
    10441 cgctccacaa cctcacgatc gaacgtgtaa agggggcttt taaggtccga aaagtaccgt
    10501 ttaacagcct gttttaaccg tttacaggcc ggaattttgc ccgtttttac gtcttctgcg
    10561 tacttattcc aggcggtcaa gctcgtcctc ttcttctgtt tccggtggat ttttacggcg
    10621 gcttatcggg tcaaaaccga gcaaggaggc catttttatc atcactcttt cagcgtcgga
    10681 ttttgcgctt aatgcggggt ttctgctctc gccgccctga ctgttaacaa tgctgaaccc
    10741 gcgcgccgca aggtctgcaa cggctttccg gtaaatggag tagttgacgc aatactgttc
    10801 cagattgctc cagtcggcgg gggtcaggtc tccccgttcc gcaagctgcc gcgatttttc
    10861 ccgccactgc ttcacggcga tatcatccag gtaggcgggg tcgggtttgt cctgaaaacg
    10921 ccccccaccc cgtcatacag cctcatcagc gattgcggaa acattccata acctcgcggt
    10981 cacggtcggt taatcgcttc gctgtgatgc gttctgcgcg tcctgacgct ttatctttat
    11041 gccctgtttc ctgtgtcttc catgcgtcac gctgccttat aagtccacgg ataaggcggt
    11101 tttgttcccg ctcattcatc agcgtcatac atccagttat tgcggttagc agcccgttct
    11161 tcctcttcac gaaatccacc tgcggcacgc ttgctttttg ttgccggatc aagccatttc
    11221 gttttctggt tatgacacgc ctggcacaat ggttgatggt tccattcggg ccagaagaga
    11281 acatcatcac cgccattaat cgggataatg tgatccacca ccacggcggg cgtatatatc
    11341 cccttctcaa ggcatcgcac gcataacggg tttttactca gatacatggc gcggtatttg
    11401 tcccactgtc tggaatatcc acgcgcgcgg cggtgtcctc gtctggcatc ctctgcacgc
    11461 catgccgccc gcctgtgctc ctcacacttg ccggatttaa cgcgcctgtt acagcctggt
    11521 tctgtgcatc gtcttaatgg ttgccacggc atcagtaccc ccccacatca cggtaagccg
    11581 tccagagtgc gcctatcgtc atgggtacgc gtgtttttgc gttatccgcg acaatctggc
    11641 gattttcata caggtgagcg ataaacatca tgcagccaat ctttatggct ggcgtgaacg
    11701 ccagcccgtc atcaaagcgc ctgcctatgt gtatctggca cgcctcaagc gatgcggcaa
    11761 tgtatccgct gattaactgg tcttcatcgt cgccatcgat gcggcaatgg agtttcactt
    11821 cttccagggt gataagttct tctgtcattt ttccgcgccc tcacgacaaa gaatttcaag
    11881 gcgtgtcctg gcggcatccg gcagcggctg cccgatgata ttcagtacac gccccgccag
    11941 cggccccgta ctgaccttta tccggctggt ggcgttgatg tccttccggt accgtatcca
    12001 gatccttacg gtaccaatcg ccagctctgc gcccgatgaa atggcctcct tgctgctgac
    12061 catgttcacg cttgcccaga gtgtgtgacc gtcctcccac gtttcgagta tttcgccggt
    12121 tatatccctg gtctgtttca gggtctgaat cgttatcctg tcacgcagtc gccctatgtt
    12181 cattccggtt tttctccctc gctgattttt acttcctgtt tccatgcctg gctgaactca
    12241 tcaccaccat cacgcggtga cagcccttca cgttcgcggg cttcgttcgg acacataacg
    12301 tcggatttta tgccgcgctc ataggtggca aagcgttcgc caggtgtggc ccgtaacagg
    12361 tccgcgctgt caaactccac ctgataccgg ataccaggta caggcgaggc caccagcaag
    12421 gcgtttttta tctgctgctc aaagttcgcc agccacgggc gcatcgtcat ggtgagaaat
    12481 gcgcggcttg cctcgctgaa gttgctgtag gtgctgttgc tgtattcctg caaaaaaatc
    12541 ggcgagacgt tgaacattcg ggcgatgtct tcaatggtga agcgacggga ggccagccat
    12601 tcggcatcct gattactcat gcccagctgc tgatagctca tccccccttc aaggatgggc
    12661 gtttttccgg cgtttctggc ccctttgtag cgttccagtg cggctaatgc ctgtttgccc
    12721 ttcacgccgt ccagccattc gcctgacgtg ataacccctg ccgccatcat gccatcacgc
    12781 atcacgctcg cgccgtggcg ttgttgggcc agcccaagcc ccagcgattc gcggcagatg
    12841 gttacaggtg agcgccccat aaaaccgtca tccgtggagt aacgaaggtg gagaacctcc
    12901 cacggtaaat agttgcggat gtttccggtg taggcgtcag tgatgcagta gcgccagtta
    12961 tgatcacctg tctgctccac gttcaccgac tgcggcgggt aaggatgtaa agccgccgga
    13021 aaaccatcac gcccccactg aataaccgca taagcattac cacttaacag gcagtagcgg
    13081 atcatcattc gcttaaactg gtagggtgtc tgccacgcgt tcgggcgctc gttgaggatg
    13141 tgatcgaccg gatgagaatc aagccattcg cgggcctcct tccccttctc attgcgtacc
    13201 aggtacaggt aacacggcat ggtagccacc gcgtcagaga tgaccgtgac ggcgttcatg
    13261 acggcgggga gtgattccgc tgtcccagat gatacgtact cacccgcccc cgtgttcgat
    13321 gtgccggcca gcgccataaa ttcatcaagc gtcatgctgc gctgctcttt ttttcgtctg
    13381 aaaggccaca tatcacaccc ccgctaaatc cgcccaccag cggcgattat ccgcacgcgg
    13441 cattttttcg gggtgctgct catacaggga acggcgggcc agctccacgc cggaatcggg
    13501 gtaagccggt accgatgtaa cggtaatttc gtacagttcc gccaccagca cggtgcgcac
    13561 gcatggatct gttgtggtat cccatacatc cttacgggag cgaaagccaa agctcatccc
    13621 cgagatgtcc ccacgcttaa ccagttcgat aacgtcgcgc cctgtactgg tatccggtgg
    13681 ggtcagttca aagcgtaacc ctgtttcgtc ctcttccagt ttcagcgtgc cggaacgggt
    13741 gcgccccagt aacatgctgt ggtcatgctc atacaggccg cgaacgtcat tacccgccgc
    13801 aagccattca gtaaacgccc cccgctggaa tttttcgtaa aactcacccc ataacagttc
    13861 tgaaagctta tcccagcgaa caacgtagcc cgtcagcgta ccggctccgc tggtggtgat
    13921 ttccgatgac cggatttcca tactcttcat aattttttca cccataaaac actaaagggg
    13981 ctttttagcc ccttctgtat gctgttaatc gtcgtcttcc ggcagtgcca gaatcttgat
    14041 cgcgttcgaa tccaccacgc caccgcctaa atatttttgc gtgaaaattt tgatgaatcc
    14101 tggttctgtc aggttgtccg gtctggtgcg aacacctgtt tcgtgatcaa caatgtagta
    14161 accgcgtttg aagtcaccca gggcaataac gttatcaggc ataaactcca gatattcgac
    14221 cggaaggccc agcaacgtat caggatcacc cgcctgtaaa cggtcgcgcc agatataatc
    14281 accgttcgcg ttcttcacct tctgaagttt tgccgccgtc gtggagttaa ccactcagac
    14341 cgcgtttttg cggtattttt tacgtaatgc aaatttcagg tcgatcagcg ggtccgcaga
    14401 tgtccacgcc agagattcgg aaggtttaat tacctgcaac gtaccaaaat cacgctcttt
    14461 gtcgttcttc tctgcacggg gtacggataa aaaacctttt gcttttttgt caccgtcgcc
    14521 cacaaccaga tcgctttctt cggtttccgt gaaggtgtcg ccaatctcac ccgtcagcca
    14581 tgaaaggatg tccacatcgg aaaaatccac gatttcctgt gtggtgcgcg ggtacgcata
    14641 gaccggatac agcttaatgc tcacctcgtt aatctgcggg gtgctggtct gttcgcgtgc
    14701 cttaccctct tcaccgtggt taacggtcgc accgccagcg gaaacaagct gcttaaactc
    14761 gttgctgctg attttcttca cggtacagat gcgacgcatg gtggattcat ccgccagcat
    14821 tcgcatgatt tcggtgttca gttcagggat aacggtataa ccaccatcag cgggaacgcc
    14881 tgtacttaat gcgcgggttt caccggtcag aatgtagtta cgtagttctg cggggtcagt
    14941 ggtctggctg cttttacctg gcttgctgcg ctcttcgtct gcaatggctt caaggcggga
    15001 aatgtcttta tcgagggatt cagctttagc gcgtaattcg tcaaattttg cgccctcagc
    15061 atcgttaaga ctgcggtttt ctttttcagc gttctccagc atgtcgcgca tctgattttt
    15121 aatggcggtt ttctgctggc gtaattcgat tattctctgc ataaaaaaag tcctggggtt
    15181 aagtaaggaa ctccaggacg cggcaaacac tcaaccgttt ttcataagga aatcagtaat
    15241 cgcaccgatc gttttcccgc ctggtaatga atattggcga gcacattaac aggcgggaaa
    15301 gtggccccag cgtcctggca ccacgggcga gaataatcat gattcagttc gggtaaaata
    15361 tgccgatccg ctcagtgaac aacgtggaac aaccacgaac aaataattta caaaaaataa
    15421 caaaaagccg gattgctccg gctgtatgtg ctgtatgggt gctcagtctc tgatcccttt
    15481 cagaaattct atcaatgcat ctgtctgttc aggatttact gccagcattt caccggatag
    15541 cgcgcaacga acaaaaccat gatgatcctt ttcaatcagc gcccccgttt ccagaaatgc
    15601 gcggtaatca gtgatattca tcgtctccat gttgtcagca tggtatttat cacgctgttt
    15661 aagtatctca tcaaatttca tcggcattgc gttttcctct gttgtgtctg tttgtttcag
    15721 atagtaacta tgccggaccg tgacgaaaag ccagtagtgc tccataccgt ttcaaatgac
    15781 gcaaaaaaag ccggattgct ccggctgcgt ggtgtggttc tgatattcct actctgctaa
    15841 tttgctcagc cccatgtctg cgtaagtgcg atttactgca tttctcaggt ctgcgtagtt
    15901 ctccgttggc ggctccggtg tgcccttgct gctctgtttt accagcgggt cgcccaccag
    15961 ctgcccgtaa gctgcaattt gtgctgtcat attgccacct cagtgaagcg atccggcatt
    16021 gttctttttc atctcttcca tcattttttc agtgagcatt acgcactgaa accccatgtt
    16081 tttgatgtct acgggtttca tgcctttgtc agtggatatc acaggcagaa tatcctgata
    16141 cacatcgcgg tcagggtaac taagcacaca aagctcccat gatgccacgg gtgaggatcc
    16201 ttcgtctgtt tcaacccacc agcctggtgt agctgcgctt atttgcagaa tcgtgatccg
    16261 tccgttgtgc ttgtaaaatt tttcaccaaa ttttggttcg ctcatgattc cacctctccg
    16321 gttttaacgt tgattgttgt tacctgttcc gcttcggcaa tctcccgctc tgtcagcgtg
    16381 gcaaaatttg ccgccgtcgt ggtcatgaat gcgcttatca gttcgggatg tgctttcgcg
    16441 tatccttccc ccgcgtggcg gtctatcgtt ctgattgcca cctttaaagc gtgctctgtc
    16501 atgtctaacg cgcgatattt cggttctgtt ctgtctctgc gttttttgaa tgatttatta
    16561 aatttccctg aagtgtgcat atttattttt accccctcgt ttaaaaagtt ttgagttgtg
    16621 cctccccttg tctaccttat ctaccttagt ggccctcatg ccagtaatgg cgctgctttc
    16681 agcggggtag agtgctttta tccactatct accccgtgtc tacctccctg tctgattcag
    16741 gtaaaatcag gtagagaggg tagatagtgg gtagacagta aaaaaaggct atctacctaa
    16801 cttaatgcac tgaattaaat gtatttttct ttactcaggt agacagggta gacagcaatt
    16861 acaaaaaatt ataaaaacgc gtcgcactcg tctgttgtta ttgcgttagt ctgcgttact
    16921 cccttaactt tccgcgtaat atattcatgt ccgtaaactt tcgcggctgg cttcatagcc
    16981 ttgccaaagt catttacgtt tagcggtttg ctcctgcctg cgtacgccat aaacgccaga
    17041 tagacgcggt aaaggctgtt tctggtcgtg tacttcactg aatcgccacc gccgcccatc
    17101 atcaggccgc gtgcttcctc cagaaaattc aggaactggc aaaactcaat aaccggatcc
    17161 gtctgttgct ttattgccag agcttcatca ccgtcacgct gttccagtag taaagcccgt
    17221 gctttctcag agtcggtaaa gttcgccagc aatcggcgga taatgacagg gatttcagcc
    17281 gcaatctttt ccggtagctc cctgtctttt tcggcctcac taacgatatt gtcgaaacgg
    17341 aaaatcacgc gacgacgtgc cacacctccg gcccgttcgg tgaatatcat cgggttattg
    17401 ttggtcgcca gcaccaccgc cctgattaca gccgtgaaac gcttttcgta tttcgggtta
    17461 atttccacgg ggtcaccgcc cgtgattttc ttgatgcccg ttccttcgcc tgtatatttc
    17521 ggctggtcag ccaggacgat aagacgactc ccgacaacct gcgcacgtcc accagcatca
    17581 tcaagcgatg tcatttcagc gcttaccgtg ttctgtttcc ctgccagaag gctggctatg
    17641 tgtgtgaatg tacttttacc gctcccgccg tctccggtgg cctcaataaa catctgccag
    17701 tcgtaccggt tcgccataat catgtacagc gcggcacata tacgcatcat cttgcgctgg
    17761 tcttttccgg ctgcgtgctc aagccattta tgaaagttta gcgcgttatc gcggatgttc
    17821 tcccctggtg ctggtggcgt gtactcaatg ccgttgtgcg tggtgatcca gttctccggc
    17881 gtgtgcgggg aaaattcccc cgttttcagg tcaagcgcac cattggcgaa cggcagcaaa
    17941 tcgccggacg gctcgcccat tggttcggca ataactttta acgcttccac ggcgttattg
    18001 attacgcgct tgctgaaagt ggccctgtgc tctgaataga tcaccaccat ttcgcggcta
    18061 agttccattg tgctgaccgg acaccatacc ccgccgcgcc atacgtgaac gatttcactt
    18121 tcctgatgca cacaaacgcc atcaaagcgc tcggcaagca gctgcgcgcg ctcactgtct
    18181 gccatttgtg ccagttgcgt atttttctgc tccggcatgg taagccctgc ggcaatattt
    18241 tcacgctcag cattcagata gcgccgccag ttttcacact tctggacgtg cattccttcg
    18301 gggtaaaaat ttgcgtcctg aatatctgcc gccgccagct tctgaccaat ctttttggtc
    18361 tccaccaaat ccagttctcc ggcctggtac agccttacgc gtttttttcc atccggaaca
    18421 attttcagtg catcaagttc ggcaagctga tttggcccaa gccacacagg cggcacatta
    18481 tcgccggatg cggggccatc ctgctcctgc cattgtttcg cgtgcgacca ggcatcacta
    18541 cccgcaaaga taattacctc tgtgtctttg tgttttattc cgcgtggctg tttttttacg
    18601 ttcggtgcca gtttcatttt ttacccctga atacgttaag catctttttt atttcctgaa
    18661 tattggcgcg tgctttctcc ctgctggttg gtttactgcg gggcgctgcc tgtaccagcg
    18721 tgaaatcacg ccggaattga taaacaggca tcacgcaatc atattcgtaa ccttcacggc
    18781 ggtaggtgat gcgccgttct gccacgcctt taatcgttac catgccgccg tactggtcgc
    18841 ggtaaatatc accgcgcgta aatttagggt gagtgttgcc actggcagtt aagccagaat
    18901 atttaagttt cattattttt attctccggt gtgttgttct ttatatctgt cgtgcaatag
    18961 atctatttct tgcagttcca ttattacagg ctcaagaagc gttattaatg ccgtggcaat
    19021 tcttgatttt tgtttgtcgc gttcattgtc gccaagtgtt tcaagccata tgcgcaatat
    19081 ttccagcatg ttttcactgt gagaaagtgc aagaaatgcg cggtctattg tttcgtggta
    19141 aatatcacgc atattaatcc ccgtccgtcg tttttcttaa aataacctct gtcacgaaat
    19201 cagcataatc ggcggcgata ttcagtacat caagcccgtt gatttatatt ctctcgtgga
    19261 aagtaagaaa aaagccgctc taataagttc tggcattgac gaaagcgcat cagccgcatc
    19321 atcaggaacg ccggaaaatt cctgtttcag ggaattaaaa cgatcatcac gcataacccc
    19381 ccccattttc acaatcggca acaagaatat ttttagcgtc attcagtgac cgcgttgcga
    19441 tgtatcggat acattccagg gcgaactgtg tccggtcttt ctgttcttct ccctgcgcaa
    19501 gctctgccgt gcattcaata tcaataagcg cgtgcatcag cgtagtgagt gcggcggctg
    19561 ctgcgttcgg tgtggtttta ttgtgcatag gtttccccct gacgaatacg ggcggcgaat
    19621 acagcaacac aaccggacgg gcaacggcta cgcgcttcgc gttccgtcca agcggttacg
    19681 tggatgattt gggattctcc ggcactcagt gccagaaaac gccacacaaa ggccgtttgt
    19741 gtgtgtacaa ggtgtggtat atgatttacg gcaaccataa cggctcctag tttacgttgt
    19801 tggttagaag ccccgttact gctcctacag tgcggggctt tgctttttgt gttgattgtg
    19861 tgtaatgtgt cactacacat aaatacatta cctgaggtgt aattaacgtg tcaacacaca
    19921 aaaacgaaag gcgtggtaat cctccgttcc agttccgttt agatcctgac ttacgggaaa
    19981 agatggaaaa ggcacaacaa caggacggcg atgattctct tgctgcctgg ataaagcgaa
    20041 taatccgcaa agaattacag caacgcggca tagaacccaa aggataaaaa ctatcagcgc
    20101 cgtggtgtga ggtactacgg cgcattgctt tacaggcgca cacaatgacc aacgaagaat
    20161 caaccgaaaa aacatcacct gcacgaaaaa gacgacgcag aaagaaagag catgaatcag
    20221 aacgattcgc gccgtgttct tttgcccttg agaagtacct taaagaatac tcatcaaagg
    20281 agagaacttc gcagttatgg caacgcactg aaccagactg attgcattgc ccaccagcct
    20341 gatagcggct atcatccccg tgcttatgtt tgggatcaca cacacaaacg gcgcagcggg
    20401 ttatctgttc agaaaggcgg ctccatttcg gggccgcttt ttttatgcct gaaaaacccc
    20461 aattttgtgg ttttccagtt tcaccagggc gaacgaatcc ccgcccacgt tctggcgtat
    20521 attcaatctt catggttata gctctgtgtt cagatgattg atgtgtggcg gctgcgtgcc
    20581 gccagcgtga ttaatgaact gccttgcagc tatccttcca ggccagaacc tcggataaag
    20641 accagccaac ggaacgaccg ccaagtttac gacgtgatgg gaattgtccg gccttttcca
    20701 ggcggtagcg gcatgagcgg ctaaggcctg ttagcttttc gcattctttt tcacgtataa
    20761 accgatcagt gcttaacact attgccccct ttcgtttctt aaagagttat ttcgtgttct
    20821 attgcgttgg gatgtgtctg attgtgtcag gatgattcag agttggcaaa tgttgaggcc
    20881 gtatggttta cagaaagagg aataatcagg ataaaatcct ttaaattcat gttaatacaa
    20941 aggcataaaa tattgtttca tgcctttttt ctcgctattt aaagagtgat tcgctagtgt
    21001 ataaaaaatc agtaacacat taaaaatcag ctacttataa atctgtacac tttttcacct
    21061 cttgttcgta gttgttccgc attgttgctc attgttgcac gttgtatctg ttcgcatatc
    21121 cagtatgcgc atactgaaaa aacacgaaaa aaattatttt cttctggcta ctggtagcgt
    21181 ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc tccgtccaca tatccagcgc
    21241 attgcgttta gcatccagat aacgggaatg attataaact cgctgcattc ctggcatctg
    21301 gtggcctgta agctgctcca cgacgtgagg atccacgcct aaatcgttca gcatcgttgt
    21361 aaaggtgcgc cggatgtcat gtagtgacca gtgagggtga tttagcctcc tgtgcgctaa
    21421 tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc cccagcaata agcccgtgtg
    21481 cctgttctgc tccaccagct gcgtgacgaa cggcagtatt gcttccggta tgggccggaa
    21541 tattgccacc ttcgttttgc tgtgttcttt cggcacggtc cagagcattt cggtaaaatc
    21601 ccactcgctg atctccgata acctcagttc taccgtccgg catccgaaca caatcaggag
    21661 gcggattaac gcgatgtagt agggggagaa tatttttttg tccagtgcct gcaataattc
    21721 gcccagttct ttggtgctta agacacgctc gcttatatcc ggttttttcc caacgtccgc
    21781 cacactcata tcatcaagaa cgttgctgat tgcatagcgc cgcctccggc agaacttaag
    21841 cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca ggcgttcgct ttgccacctg
    21901 gtcaaaacag gccagccagt gccgtagctc gcatttatcc agcggcatag caccaatgtg
    21961 ctgtattacg tgattattaa ggcgcttttt cagggcggca taatccacgc ggttttcctt
    22021 tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc gttaccggct ttaacgcttc
    22081 ctgtacggtg taattaagct catgacgtgg atttttcccc tctgccagcc atgcgcgaca
    22141 ctgggcggct ttttccctgg ctgatttcag gctcagatca ggataatttc ccagcttaat
    22201 gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa gtgaaatacc aggttaaaag
    22261 gccactggtt gaatgcctga cgctcaggtt tccaccgtca ttaagaaagg ctgttttttg
    22321 ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc agtttgttaa gtgctctgct
    22381 cataattttg attccggtca tcacaattaa ctacaccatt aactacaccg atcgttgcac
    22441 aaagggctac aacgtgaaac aagctggaac aaggaaaatc acaaatctgt ttataatcaa
    22501 aaacataatg aacaatctga aacattatga aacggcaaaa aacactaatt gataaaatat
    22561 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    22621 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    22681 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    22741 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    22801 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    22861 ccaactggtc attactggta caggcagtaa accactggca tgagccgacc gccgcgctga
    22921 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    22981 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    23041 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    23101 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    23161 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    23221 actattccgt gggttttcgc gcgccaaata cgcgggaatt aattagcgga tttgccgatt
    23281 atgtgctgca acgtgaactg ggcggcaact actacagcga tccggatgtt ccacctcgtg
    23341 ctcatcctgc ggatgttctg ccgcaagaga tggataaact gcgtgagatg atgctcgaat
    23401 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    23461 atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc tacgatgcgc
    23521 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    23581 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    23641 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    23701 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    23761 tttaagccgg atgcagcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    23821 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag aatcacaaac
    23881 tcatgcttac catgatagtt gtaaccgcca gtgaacaggt tcgggcaagg taatcccata
    23941 aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg
    24001 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctcg
    24061 cgcatattgt agtaactgtc ttcaatcacc aattcaatgt agcaatcagg atgtaaccct
    24121 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    24181 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    24241 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    24301 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    24361 tgaacattgt taccgacaat ttttatattg accgatgcgg cgttgaagtt ttcaaactcc
    24421 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    24481 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    24541 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    24601 gctttgtcat cagcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    24661 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    24721 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    24781 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    24841 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    24901 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    24961 ctcacccctg cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt
    25021 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    25081 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    25141 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    25201 gaattaccgc cctcattagg agtcaccaag gtggttaacc acaaaccccg catcggtaag
    25261 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    25321 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    25381 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    25441 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    25501 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    25561 tccaaagcta tgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    25621 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    25681 tgcagctgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    25741 gcgtcgccat tgctcgagcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    25801 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    25861 gtaagcttgg cattacattc gttttcgtga ctcacgacca ggaagaagca ctcaccatgt
    25921 cggacaggat tgtggtgatg cgcgatggtc gcattgagca agacggcacg ccgcgtgaaa
    25981 tctacgaaga gccgaaaaac ctgtttgttg ccgggttcat tggcgaaatt aatatgttta
    26041 acgccacggt gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    26101 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    26161 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    26221 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    26281 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact
    26341 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    26401 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    26461 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    26521 agtttcgtca aaatggtctt tacactggat aactacacgc gtctgctcga tccgctctat
    26581 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    26641 ctgggctatc catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    26701 ctgtttctgc tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa
    26761 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    26821 acaccaatcc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    26881 ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    26941 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca
    27001 ctgacgatgc cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    27061 ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    27121 gtacagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    27181 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct gaataagaag
    27241 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    27301 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    27361 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    27421 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacgttt gctacgctta
    27481 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgcggtaag ccgttcgtta
    27541 gcggaatgct gtttgtggta atgatgtcac cagatatcgt gatggcaatt tcgctgctgg
    27601 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    27661 ccttctgcct gccatttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    27721 ggatgctgga agcggcgaaa gatctcggtg ccagcgaatt taccattctg cgaaaaatca
    27781 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    27841 tggacgatgt ggtagtttct tcattcgtca ccggaccaag ttatgaaatt ctgccgttaa
    27901 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    27961 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    28021 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    28081 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    28141 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    28201 ggttatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    28261 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    28321 ggggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    28381 catgctcaac aagccttttg acccgaataa cgactactcc attccgtata tctggggtgc
    28441 gacggcgatt ggtgttaacg gtgatgcggt ggatccgaaa tctgtcacca gctgggccga
    28501 tctgtggaag ccagagtaca aaggcagcct gctgttgacc gacgatgccc gtgaagtgtt
    28561 ccagatggcg ctgcgtaagc tgggctactc cggtaacacc accgatccga aagagattga
    28621 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    28681 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggttctgc
    28741 tttcgttgca cgccaggcgg gtacgcctat tgacgtggtg tggccgaaag aaggcggcat
    28801 attctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    28861 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgctgaaa ctatcggtta
    28921 tccaacgcca aaccttgcgg cgcgtaagct gttaagtcca gaagtggcaa acgataagac
    28981 gctctacccg gatgctgaaa ccattaaaaa tggcgaatgg cagaatgacg ttggcgcagc
    29041 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaagcg
    29101 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    29161 taaagtttta tcccgctctt tcgtcggtat tgtggtccgc ttgtaggcct gataagcgta
    29221 gcgcatcagg caatgcttcc tgctttataa tccctccagc aacttttcaa caaattctgg
    29281 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccaa cctgactcgg
    29341 ttcaagattc agttcaacgg tgtgagcacc atgtagtttg gcttcgtgta caaacccagc
    29401 cgccggatag acatgcccgg aagtaccaat ggcaataaag acatccgcca tcgacaacgc
    29461 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    29521 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    29581 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    29641 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    29701 atcgccgaga gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    29761 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    29821 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    29881 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    29941 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    30001 acgctcgcgc aggcgacgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    30061 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    30121 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    30181 acaaggcaac ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    30241 aatgacgacc aggtcagggt caacaatggt caggatattt cccagagaaa ccgctaataa
    30301 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    30361 aatttcggga gcctgcaacg gttgatgata atagtgttga tacagccacg caaaaccgcg
    30421 accagacaga taattttcaa tgcagccatg ctgaccacag ccgcagcggc gtaacgggaa
    30481 atccaacccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    30541 aatgtagctt ttaccagtaa tcggtttgcc gttgaaaacc agcccgccgc caacgccggt
    30601 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    30661 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    30721 gtcggcacgc agcggtttgc cgctggcagc ggggacatta gcggcataca gcttaccatc
    30781 ttctgtttcc ggcattcccg gaataccgat gccgacagaa cctttacagc caaaacgttg
    30841 gtcagcttca gctaccagct cacacactgc atctaaaaat gcgtcatagc tgtcacgcgg
    30901 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    30961 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    31021 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    31081 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    31141 gcaattccga tggcaggaag tcaataaaat aaatatcgct ggagaggaac tgatgaccga
    31201 tcagcttttc aatccactca ataatcgggg taagttgcag tgaaacaact acgccgataa
    31261 ccaccccaca caggctgccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    31321 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    31381 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    31441 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    31501 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    31561 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    31621 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    31681 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    31741 cgggattcga gttggggatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    31801 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccacgcat
    31861 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgtggg ttaacgccct
    31921 tcacctggat tgcgcgcaga ttcgctccac tttccaccag accggtgaaa ttgatatacg
    31981 gcgcagcggc ggcaataccc ggcactttct gcacgttatc cagtgcttcc tgccagttag
    32041 tccatggctg atttaccgct tcgatttcgc catgcggcac caccgccaga atgcggttat
    32101 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    32161 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    32221 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    32281 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    32341 cgccagttgc aggtcgtgag tgaccaccag gaaagcggtg ccctgcaagc gattcaactc
    32401 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggttcatc
    32461 cgccagtacc agacgcggat tattgaccag cgcacgggca atcgctacac gctggcgttc
    32521 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    32581 catctccagt gcacagctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    32641 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    32701 cagcttctgg ttgcgcagtt ctgctttcgc cgctgaagac agtttgctca tcggctgacc
    32761 gttaaagatc acatcgccgg aggttggcgt atccagcccg cctaacaggt gcagcaaggt
    32821 acttttaccg gaaccagagc taccgacaat tgccatcatt tcaccttcgc ccacgctgaa
    32881 actgacattg agcagcacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    32941 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttgt gtggcggcag
    33001 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    33061 caatgacctg taaaggttcg atagccaccg gcagcgccgc gccatcaagc aggacgccga
    33121 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    33181 ttgcaccgat aatcccggcg ctagcccctt gcaccataaa gaccatcatg atttgtcgcg
    33241 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatc accatcagcc
    33301 ccagcgaggt aataatgtta aacgccgcaa cagcgacaat caggctcagc agtaagccca
    33361 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    33421 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactctc agcggctcat
    33481 ccagccacaa acgccagccg gtaatattgg ctgccggata acgcatcaga cgcgaggcat
    33541 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaagtgccaa
    33601 tcacattgaa caggcgctga cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    33661 gcaccatcac gcggatttga tcaccgcgat tgacgcccaa ctgtgaggca agttgttcgc
    33721 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    33781 gcgttagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    33841 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    33901 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    33961 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    34021 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    34081 agacgaaacg accgaagcga tccactgcac gcccacgcat gtaacgcagg ccaataaata
    34141 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    34201 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    34261 cccccgtaaa tcaatcggct aaattgccat attttatcat caatcttgcc gcctgatctt
    34321 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taaccagcag
    34381 gatcaaccgc atgtaaatta aaaaagagcg tgttgttctg atgaaacaaa aagagctatg
    34441 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    34501 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gcaaatgctg
    34561 gatctatttc aatctttacc ttgccccctt tcgtatgccg gtttttttct ttatatccgg
    34621 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    34681 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    34741 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    34801 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    34861 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    34921 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    34981 aatgaacagt gtgatacgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    35041 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    35101 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    35161 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    35221 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    35281 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    35341 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    35401 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    35461 ttcactgccc gctgccgtca gttactctcg ttaaaccatc accccagccg gggcgattca
    35521 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    35581 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    35641 atacgctgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    35701 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    35761 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    35821 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    35881 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcatactga
    35941 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    36001 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    36061 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    36121 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    36181 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    36241 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    36301 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    36361 aagtgagtaa aggcacatta cctgctggga tcgagtactg gcagccattg ttcttcagcg
    36421 aaccactgcc accgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    36481 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    36541 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    36601 tcttctcaga gctgaaaaac tggccccgag tgcagctaaa aactgaacat ttaccgacaa
    36661 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcgcaac
    36721 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    36781 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    36841 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    36901 ttggcgctgc cgaacatggt tttgtcgata aggcgcgtaa tctggcgctg atctgcgaaa
    36961 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    37021 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    37081 agcacggcgt cggtcgctat gccggaatga ccacgctgga agctggcggc attactggcg
    37141 agtacctgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    37201 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    37261 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    37321 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    37381 gtgagcagta tcagttattc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    37441 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    37501 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    37561 ataaccacaa gcaggtagcg gtgttggtgc ctaccaccct tcttgcgcag cagcattacg
    37621 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    37681 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    37741 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    37801 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    37861 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgtacgctg aatatggcaa
    37921 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    37981 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    38041 tgcgcggagg acaggtttat tatctctaca atgatgtgga aaacattcag aaagccgccg
    38101 aacggctggc agaactggtg cctgaagcac ggattgccat cggtcacggg cagatgcgcg
    38161 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    38221 gtaccaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    38281 gtgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcac
    38341 atcatcaggc atatgcatgg ctgctgacgc cgcatccaaa agcgatgact accgatgcac
    38401 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    38461 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    38521 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    38581 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    38641 ggatgccgtc gctattgcca gatgatttca tccctgacgt gaacacgcgt ctgtcgttct
    38701 acaaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    38761 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    38821 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    38881 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    38941 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    39001 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    39061 tcgcttaatg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    39121 gcactttcct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    39181 caataacctt atatttatta tgtatttttg gtgatgatca aaacgcgttt ttctcgctgg
    39241 ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc
    39301 tggccgctgc cgccagcgag cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    39361 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    39421 ttacaagcta accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc
    39481 ccgttgcaaa ccctacttcc agacgcgccg cgcgaaggca ttgtgatcaa cattgcggag
    39541 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc aataggtatt
    39601 ggtcagttag gtggtgacac gctgacaccg acaatggtga ccaccgtttc agacaaacgt
    39661 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    39721 gagttgcctg cggtagtgcc ggctggaccg gataacccaa tgggccatca tgcgattcgt
    39781 ctggcggcct atggcggcgt ttatttgctt cacggtacga acgccgattt cggcattggc
    39841 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    39901 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    39961 ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    40021 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    40081 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    40141 cgccgtcatc aagtgagccc acaaactctg taaatcctgg ataaaaaaaa tccccgcgac
    40201 acggcatgtc gcggggaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    40261 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    40321 attttgcgcc catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg
    40381 tccccgcgtt agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    40441 cgacagccgc aaagttggca aatgacatgg agcttaaaat cgccgcagcg atgaggtttt
    40501 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa ttacttaacg
    40561 atcgataact aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc
    40621 attaattaat ttaaaaatct ttaaatttca gtcaaataga aagcaattat ttttatggga
    40681 tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg
    40741 tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca
    40801 aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt
    40861 tatgaagcga cttctcttgc ggacctcgtc gaagcaaccg gagccaaagc gcccacgcta
    40921 tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat
    40981 cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg
    41041 ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc
    41101 tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat agccaatacg
    41161 ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa
    41221 gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt
    41281 attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actgatgcag
    41341 attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaggcc
    41401 cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat
    41461 tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt acccggatcg cggtccatgc
    41521 ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg
    41581 acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga
    41641 gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga
    41701 catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca acagcgccga
    41761 gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt
    41821 tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct
    41881 gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct acgcccaacg
    41941 cagctgcgac accaatcccg atacccgcca acattgattt attcacggga acacctcctt
    42001 ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga
    42061 aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg aatgcctgat
    42121 gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggataag
    42181 gcgtttatac cacatccgcc agtgtacgtc gattaatgca acttcaaacg cggacggata
    42241 acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca
    42301 atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc ttcaatcatc
    42361 attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt cgacagcgat
    42421 accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt catctgcgcc
    42481 agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg cggcggaaca
    42541 aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg
    42601 cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt ttcaagacca
    42661 ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat cagatcagcc
    42721 tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt
    42781 tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc agagatacgt
    42841 ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt cagggcttcg
    42901 ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg caattcagcg
    42961 gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt gccatttgcg
    43021 cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa gcgacgcgcc
    43081 tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt gaaatcgtta
    43141 gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc cggaaccaac
    43201 agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc acgatcaata
    43261 tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc cagatagctc
    43321 aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag tggtttccac
    43381 aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg tcccagctta
    43441 tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaatcttt
    43501 ttcaatggcg tagtcaacgt gcccccctta aatttattaa ccaattgtta attaatggtt
    43561 attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag
    43621 aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctta
    43681 aaagataggc aactttaccg ggggagatca aaaaatagag tttattgttt attatttgag
    43741 caaattcgga agggtaatat agggtgaagc gtatatcgag ggcgtttggt cgtcctgata
    43801 agatgcgcag catcgcatca ggcattgatg ctcgactgtc ggatgcggcg tggacgcctt
    43861 atccgactta cagaattcgg agcgcatcag gtaccggcaa ggttacctga tgcgacgagg
    43921 gcatttaccc gagggttttg aacgctttaa tgcgctgtaa atgcggggag atattcttga
    43981 atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac tcttcagatg
    44041 tccgctggct gttaagtgct tcatcattac gcgacaaaat aaccagacaa cgatcgcgat
    44101 tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct tccgggcgat
    44161 caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc acctggcgga
    44221 tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc caggccaacg ccgcaaatta
    44281 gcggacgctc gtcaacgttc agttgcaaca ttttgtccac ttctttaagc agatgctgca
    44341 tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg tcaataaact
    44401 gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg tgtaaataga
    44461 taatcatcgc ttcctcacat aagccagggt tagtgaccta ctttctcttc ctgccagcgt
    44521 tcatgtaact gattcagacg ggtgctgatc gctttccagc gagccgagtc catcagttcc
    44581 tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac gttctgcctt gatcggtgac
    44641 agattatcta acacgctgac ggccccttta cgattattgc agaccaggat catatcgcaa
    44701 cccgcatcca gtgatgcctg cccgcgttcg gcataactgc ccataatagc ggcaccttcc
    44761 atcgataaat cgtcagagaa aatcacgccg tcaaacccca gttcctgacg caaaacggtt
    44821 ttcagccagt agggagaacc gctcgccgga cgcggatcaa catcactgta gatcacatgc
    44881 gcaggcataa tggcgtcgag tttattttcg cggattaagg aactgaagac cgacatatct
    44941 ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc
    45001 gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc ttcatgcata
    45061 ccatcaataa accggctggc aattgccagg gctttttgtg gatcggcatg ataagaacgc
    45121 tcgccaatcg ccgcgctgat atgcccgaca tccagcactg gcgcaaagct gatatcaata
    45181 tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag tttgccgccc
    45241 tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg
    45301 cgaaaacgct gcacgcgtcc accttcctga tctaccgcca ccaccagatg attgcgcgat
    45361 gctgcgcgga tctggcgcac cagttcacgt aactgggcag gatcatgata gttacgcgta
    45421 aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc cgcgtccagt
    45481 tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt gttttattaa
    45541 tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg tttgtcgcca
    45601 gcggtactca aaccaccctc ctttgagcat cagcagccag ggaaaccatc gcctgacctg
    45661 acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga ccaattgccg
    45721 gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat caccatctcc
    45781 agcatactcc cagtcgatga gttttaaccc tgatgcgcta tgcactaaat ttccggcgtg
    45841 gacatccata tgcaatggac ttaagcgtaa aggtcgtggt tcccgcgctt tgcgcagacg
    45901 ttttaacatt cgcagccaac ccacagtccg ccgcgccgga tcgctttgct gccagtacag
    45961 ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat gtagataata
    46021 cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttaccgcac cgggcagata
    46081 gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag
    46141 ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg
    46201 ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga aaactaccgc cactaagccc
    46261 actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg
    46321 cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt tcgcccgtct
    46381 gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag gcgcaggagt
    46441 acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta cgggtgccta
    46501 aactgtcctg cggcgacaaa cctaactgtt gcttagccat cgacagttgc tgggcggaaa
    46561 ccagggtaaa tttcccgtta ttagccagcg catttcgcag cgtttcggtc gcttctgcgg
    46621 cattcagcga accgttagta cggttgttaa cgctatcaac cagcaggacg ctacccgcag
    46681 tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca ttccagtcat
    46741 aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc gtgctcaatt gggcctggct
    46801 gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc tcggctggtt
    46861 gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc acacacccgg
    46921 cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc atcaagattc
    46981 acccctttac aaatatagat aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc
    47041 gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg
    47101 catctccagc cctctggcgt cataccagta aaaacgataa tgaacggtaa cgggttcttg
    47161 cctttcgtta taaatcgttg aggatgctga aggttgaata tcagacgtcg aaaggacggg
    47221 cttttctgca ctgatgcccg cagccaataa agatgactcc atcaccagcg attgctcatc
    47281 attcaccgga atttccggat gtgaacgaca gcccaccagc agtaacaaca ccagagacac
    47341 cagcccaaag catccttttc tcatcgttac agacctttat gcgccagcat tggtcccagc
    47401 ggacggccac ccaacaagtg catatggatg tggtaaacct cttgtccgcc atggcggttg
    47461 gtattcatga tcagacgata gccatcttcg gcaatacctt cttgctcagc aatttttgcc
    47521 gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc
    47581 ggaatgagaa tattcggaat gatcagaata tgcgttggcg cttgcggcga aatatcgcga
    47641 aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg acgaataatt
    47701 ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg
    47761 ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac ctgctctttc
    47821 aatcttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat atcgccatta
    47881 ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt acacgacaaa
    47941 tatgaatgcg tatatttctc atttgcattt gcatttgcat ttgcagcaaa attattcgca
    48001 cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa cataaaccaa
    48061 gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag tcatcaccaa
    48121 accgtcacta cttgccggtt gcatagcact ggcactatta ccttctgccg cttttgctgc
    48181 accagccact gaagaaacgg tgattgttga gggttcagcc acagctccag atgatggcga
    48241 aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga ctcaacatga
    48301 tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc agttacaaac
    48361 gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg attccgatcg
    48421 tgctctttat tattcccgcg gattccagat cgataactat atggttgatg gtatccccac
    48481 ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac tgtttgaacg
    48541 cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc catctgcggc
    48601 aattaatatg gttcgaaaac acgcgacctg tcgtgaattt aaaggcgatg tctcggcgga
    48661 atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac tcaccgaaga
    48721 cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat ggctggaccg
    48781 ctacaatagt gaaaagacct tcttctcggg cattgtcgat gctgatttag gcgatcttac
    48841 gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccaa cctggggcgg
    48901 tttaccgcgc tggaatactg atggcagcag caacagttac gatcgcgcac gcagtaccgc
    48961 acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc tgaagcagcg
    49021 gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg aatttgacag
    49081 caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg ttgggccata
    49141 cagtaattat ggacctggct ttgatcatgt cggcggcacc ggttggaaca gtggcaaacg
    49201 taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg gtcgtcagca
    49261 caatctaatg tttggtggca gttacagcaa acaaaacaat cgttcagttc atgggccaac
    49321 atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc acaagccgac
    49381 tggtcatcac agagcctggc gcaggacgat accacacata tgaaatcgtt atatgctgcc
    49441 actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta taccaactgg
    49501 cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta cgctggtctg
    49561 gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat tttccagccg
    49621 caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa caactacgag
    49681 ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc catcttccgt
    49741 attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag caacggcgaa
    49801 accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga actcaacggc
    49861 gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatcttgc agaggataac
    49921 gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt caccagctat
    49981 cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca aaatcgcgtg
    50041 tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag ctacgcgctg
    50101 gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg gaacgtcaat
    50161 aacctgttcg acaaaaccta cgataccaac atggaaggtt ctatcgtcta cggcgcaccg
    50221 cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa aaaggcagcc
    50281 atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat gtactcatcc
    50341 atctcggttt tcaggttatc ggatttagta ccgaaaatag cctgaacacc agaaccagca
    50401 accactacgc ccgctgcgcc cagtttcttc aggccagcct gatccacttt agacacatca
    50461 gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt ttctttacca
    50521 ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgttgcttt tgcatcttca
    50581 gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac gcggaagatg
    50641 gtgtagtaaa caatcgcata accgataccg acgatcggta acagccacag tttgctgctg
    50701 ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc gtcacgcatc
    50761 cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat gatgtacagg
    50821 atcggcgcaa cgaacatgaa gaagaactcg atcggctcgg tgataccggt caggaacgag
    50881 gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg tttagcagag
    50941 tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa gccaccagac
    51001 agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg gaaaacctga
    51061 cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca gatgtggtgc
    51121 agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc aactaccggg
    51181 ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg cggccaaatg
    51241 aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg cacaaagcgt
    51301 ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg gttaaacatg
    51361 tacgctgcaa tcgcaccgga gataatccct ccgagtacgc cagtatccgc caagtgttta
    51421 gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc catggtttta
    51481 accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt attggtaaag
    51541 ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga accgcctgct
    51601 tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga accgacgccc
    51661 agcagaatac ctgcgatagg tagtacggat accggcagca tcagcgattt accgaccttt
    51721 tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta tgggtgcttt
    51781 ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg catctaagcg
    51841 ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac ggctatcacg
    51901 tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt cacaatattt
    51961 caaacgcggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt tagccattaa
    52021 ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc gtcgatgtga
    52081 aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc aacacctttc
    52141 aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc ttttcctcga
    52201 tgcggtaccg gcgcaaggta aggtgagtca gtttccacca gtaatcgatc cagggggaca
    52261 taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc ggaaaaggag
    52321 atgtaaaatc cgagatccag taatttaccc gccgtttctc tgtcctctgt aaaacagtgt
    52381 agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt atcggcacgg
    52441 gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg gatatgatgg
    52501 atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc cagcccggtt
    52561 tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc tacatcgtag
    52621 ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc acgttcgcct
    52681 accagatccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag acaaaatttc
    52741 acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa agattcataa
    52801 tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct cttaaagatg
    52861 aggaaccggt aacacaacgc ccggttgcag gtaatgctca atacgcagta aaagatcggt
    52921 gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa tgtggcaaac
    52981 atcccccagt atagcctgca ggcacgaggg agaaagatgg tttgccagtt cggcgaccag
    53041 gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta gcgcatccat
    53101 cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa gggccgccag
    53161 cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca atgtttcacg
    53221 agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac cggcgcttaa
    53281 gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa gccaagtcac
    53341 ggcgtactgt tctggaggcg gcgcaaggta atgtaaccga caacgactac gtaatgttgc
    53401 cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg gtggctcttc
    53461 aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat cggttaccca
    53521 gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga cctcacgtac
    53581 cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt aatcgggatg
    53641 cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt ggccctgcgg
    53701 ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc ccatgcccgg
    53761 taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg ctaccagttt
    53821 ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt cacccagtgg
    53881 gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc atcaatggta
    53941 tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg attaaagaaa
    54001 tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg ttttaagcca
    54061 acttccgggg taacatcgag atagagcgtt aagtctgggc gaaaatcccc gagaacagca
    54121 tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg atacgcctga
    54181 gtggaaagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc aggtttgatg
    54241 accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc tttatcggta
    54301 atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt ttcggcaagt
    54361 tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg ctcgagcgtc
    54421 tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc aatgacgata
    54481 tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag acttgttatg
    54541 actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa aatagagata
    54601 cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg tagctatcgc
    54661 acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt ccaggtctgc
    54721 acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg ggtcggtctg
    54781 caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat cgcgttcact
    54841 ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat ttttatcttt
    54901 ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca ccattttctt
    54961 gtgaactcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatca aggtgtctgg
    55021 ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg ctacggtggc
    55081 gtacttatcg tcactcagcg tgtgcttgat atacggagcc tcacgcaatt gcttgaggta
    55141 gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt taccgctttc
    55201 caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag tcccggcttt
    55261 aaagtgagaa agatccggtt cgatacgcag cagccattga aaaacccgtg ggcgattgat
    55321 gatcttatca gcataaagct gttcaccgag cgccagacgt ccggtccctg gcttcagggt
    55381 aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa ccttccagac
    55441 gcccacacca gcggcgatac ccagtaccac cagcaataac aagataatca ataacacttt
    55501 tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa cgttgctgac
    55561 gaaaaggtga catcgccaca ggcacgtacg ggcatcactg gcattaacgc attacaaata
    55621 accatctcat ctgcctgcaa cgcctcctcc agcgaggctt gcacttcgac aagctgataa
    55681 gaggattgtg ccagtaaacg gatacagaat tgtcgcataa tgccgttaac acctgcctga
    55741 tccagtcgcg gcgtataaac tacattgccc ttccgccaga acaaattagc cgcacagcat
    55801 tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt ctgctcaaga
    55861 tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt taatacctgc aagatgagga
    55921 ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg caaacggtcg
    55981 taatgtgcag gataagccgt aacggagaga atccgcgttg ctggtccgct gttcaatgtg
    56041 ctgtaccctc gcccgccact accgcgactg atcacgactt tcagcacacc attttgctgt
    56101 tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca ggaaatcatc
    56161 aaccgctgac aagcatcctg tagtcgcagg atatgcgccg ataacaaact gactttaccg
    56221 tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg atcgcttact
    56281 gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta tcagagaatt
    56341 gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag aaaaaaggcc
    56401 cgcaagcgga ccttttatat gggtggaaaa tgacaactta gatcttttta aagatcaaag
    56461 aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt ccgctgacct
    56521 gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta tccaggttga
    56581 tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat tctactgcac
    56641 ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac acacggcttg
    56701 cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca gccggcgtag
    56761 aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca tcacgcagag
    56821 catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc atatgataag
    56881 catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg cgttttttcg
    56941 cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg aagccatcac
    57001 gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta gataatgcac
    57061 gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc tttctctgcg ccacctgcaa
    57121 ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg ttgtgcacgc
    57181 cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac atgatagtca
    57241 gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg atcttacgtg
    57301 gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg ccaatcccgg
    57361 agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg ccagaatcct
    57421 gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc ttgcgctgtt
    57481 ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca aatttcgttg
    57541 cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg gcaagcagag
    57601 ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt ccggtcacaa
    57661 ctacacgacg cttagacacg tttgtcctcc aaggagggaa aaatgattct agtgggacaa
    57721 aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc
    57781 gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat
    57841 ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc
    57901 gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg
    57961 ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc
    58021 aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa
    58081 tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa
    58141 atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg
    58201 tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat
    58261 gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc
    58321 gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg
    58381 tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc
    58441 aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt
    58501 ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg
    58561 acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt
    58621 agtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt
    58681 gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa ttttttccag
    58741 aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc cgttggcacc
    58801 taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa taactttcgc
    58861 gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac
    58921 cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga gcgccgctgc
    58981 catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag
    59041 gccagtaagc accttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgccat
    59101 gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg
    59161 gatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag aaactgttgg
    59221 tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg gtttcatcag
    59281 cgcacagtga gacggtacgc tcactggtaa cggcagcgcg cgttttgcgc cagcagcttt
    59341 acaggcagcg ccagcacgct caaccgcttc tttatgaccg gcaataacca cctgtcccgg
    59401 agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac acgctttcgc
    59461 aataggcgca tcatccagac cgatgattgc cgccatagcg cccgtgcctt ccggtacggc
    59521 ttcttgcatg aacttaccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac
    59581 gccagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca ttgccggtgc
    59641 tttaccgccc tgctgctgcc atacgcgata cagcgcaaca gatgcagtca acagcgctgg
    59701 ctgggtttgc caggttttat tcagttcttc agctgacccc tgctgggtca gcgcccacag
    59761 gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc
    59821 cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa
    59881 ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcagagcccc aagtgaatcc
    59941 accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt cgcgtacagc
    60001 ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac
    60061 cacgacattg tccatcgaca taccgagttt tttcgccgtt gcactgataa tacgcaggtt
    60121 agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg ccgccagcgt
    60181 ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt tgcccgccat
    60241 cgtcagatga attgaattct ctggattcac gcggtcggcg tttggcagcg tcagcaattc
    60301 accataacta ccgtcggcat gcagatgggt ggaaatgatt cccggctctt cagaggcagc
    60361 cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat ctgttggatc
    60421 gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt
    60481 cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcacaggctg ctgcaacgtc
    60541 aaatgccggg caacctttaa tgtccaacat gctttgaatc tgacaagctg cgctcgggaa
    60601 agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc
    60661 cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg
    60721 cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc
    60781 caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc
    60841 aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa
    60901 ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca
    60961 gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc
    61021 gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac
    61081 catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg
    61141 ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta
    61201 gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga
    61261 caacaccttc catcgtcttt aacgtgacat ttcctgtaaa gccgtcacaa accagcacat
    61321 ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg atagaaggga
    61381 ttgttttaag caccgctgag gcatcccgaa tactgtcaag accctttact tcttcttcac
    61441 caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca gccagaactg
    61501 agcccataat ggcaaattgc accaacattg tgctgtcaca atcgacgttg gcccctaaat
    61561 cgaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc gccggacgct
    61621 caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc ccggtattac
    61681 cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc atacgcattg
    61741 aactcccacg gctggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact
    61801 gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca agtaatggcg
    61861 tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa ttcagtgcct
    61921 gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca tctaacgcca
    61981 gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg
    62041 aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga ccttgcggcc
    62101 gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc
    62161 tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga
    62221 acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac ggtttatccc
    62281 cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg caaatgggtt
    62341 tggcttttgc acttcttcag gcagttcacc aaagaccatg tccgcttcgg acacttcaca
    62401 gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat cttcaaccat
    62461 tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg cttccggcag
    62521 tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt agacctgatg
    62581 agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg catcgccgtt
    62641 taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca catcactgtc
    62701 cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga taccctggta
    62761 atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt ttaccttttg
    62821 catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg cagcctgtgg
    62881 ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc gttcagtgct
    62941 gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat catcaatacg
    63001 ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc gccatggcgt
    63061 cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcacctga ggtcgacgaa
    63121 accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca agaaaaatcg
    63181 caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca ggtatgtgtt
    63241 cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct gcaattacgc
    63301 aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa ttcggcgaat
    63361 gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct aagcgaggcg
    63421 gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag ctttaagagt
    63481 gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa cacgctggtt
    63541 ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg
    63601 ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc aggcggcctg
    63661 atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat gcttgtatta
    63721 gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct tcatccatcg gcgcttcgat
    63781 acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt gcaggaacag
    63841 acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt caccgtagcg
    63901 atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct gatgagtacg
    63961 tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt ccactttaaa
    64021 gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc cgctttgcag
    64081 aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact gaccgcgcac
    64141 cagcgccagg taatcttttt gcattccttt ttcacgtaat tgctcatgca gagaacgcaa
    64201 cgccgagcgt tttttcgcta ccagcaacac acctgaagtg tcccggtcga gacgatgaac
    64261 cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa cgccgaagct
    64321 taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatatgatc
    64381 atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc
    64441 ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag
    64501 tttatattca ggcttaatac gttttttgtt cacccgcact tcacctttac gcaaaatacg
    64561 gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat cgatacgttg
    64621 ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt
    64681 gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct tatatttagt
    64741 tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa ccaatcacca
    64801 gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata
    64861 acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa
    64921 ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta
    64981 ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat
    65041 ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc cgcatgaaaa
    65101 acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg
    65161 actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc
    65221 gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc
    65281 aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa
    65341 gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac
    65401 cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa
    65461 aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta
    65521 aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca
    65581 acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc
    65641 caaataaccc gcgcgcaggt ggcatttctc gccgtatcga aggcgatgac cgtaccgaat
    65701 taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg
    65761 ctggcgtagg caaatctgct gaggcgctgc aatgggattt aagcttccgc ctgaaacact
    65821 gggaagccat caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt catcaggaga
    65881 gcaacgtaat cgttcgcgcc ttccgcgatt atttacgtca ggatatcggc gaaatcctta
    65941 tcgataaccc gaaagtcctc gaactggcac gtcagcatat cgctgcatta ggtcgcccgg
    66001 atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc cactaccaga
    66061 tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc ggtggttcca
    66121 ttgttatcga cagcaccgaa gcgttaacag ccatcgacat caactccgca cgcgcgaccc
    66181 gcggcggcga tatcgaagaa actgcgttta acaccaacct cgaagccgcc gatgagattg
    66241 ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga
    66301 cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc
    66361 gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgccagc
    66421 gcctgagccc atcactgggt gaatccagcc atcacgtctg cccgcgctgc tccggtaccg
    66481 gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtttgatc gaagaagaag
    66541 cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc gcttcttatc
    66601 tgctgaatga aaaacgttct gcggtgaatg cgattgaaac gcgtcaggac ggtgttcgct
    66661 gcgtgattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgttg cgcgtgcgca
    66721 aaggggaaga aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg
    66781 cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc tgaacaacct gcactggcaa
    66841 cctttgccat gccggatgta ccgccagcgc caaccccagc tgaacctgcc gcgactgtcg
    66901 tagccccagc accgaaagct gcaacggcaa caccagcagc tcctgcacaa cctgggctgt
    66961 taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa gccaaaccga
    67021 ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaaac
    67081 ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga cacccgtagt gaacgtactg
    67141 aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag cagcaaactg
    67201 ccgagacgcg tgagagccgt cagcaggctg aggtaacgga aaaagcacgt accaccgacg
    67261 agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc
    67321 aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag
    67381 aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt
    67441 acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa actgccgctg
    67501 ccgaaccaat tgttcaggaa gctccagctc cacgcacaga actggtgaaa gtcccgctgc
    67561 cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat aaccgtgaca
    67621 acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc
    67681 gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc gccaatgccg ttgaccgtag
    67741 cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatcca attgtacgtc
    67801 cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga agtacaagtg cagccgatgg
    67861 tgactgaggt ccctgtcgcc gccgctgtcg aacctgttgt tagcgcgcca gttgttgaag
    67921 aaatggctga agtcgtagaa gcccccgtac cggttgccga accgcaaccg gaagtggttg
    67981 aaacgacgca tcctgaagtg attgccgccg cggtaactga acagccgcag gtgattaccg
    68041 agtctgatgt tgccgtagcc caggaagtcg cagaacacgc agaaccgatg gttgaaccgc
    68101 aggaagagac ggcagatgca gatattgaag aagttgccga aactgctgag gttgtggttg
    68161 ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc gcagcagaag
    68221 ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga gcataaccac gctaccgcgc
    68281 caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac agtgactggc
    68341 agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg gcaacgcatc
    68401 atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct caaagtaatc
    68461 aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata ttaaaattca
    68521 tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaaatgc ccgccccggc
    68581 gatcccagag cgggcagaca aaacataccc agtttcatga tatgttttaa agcgagctta
    68641 tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat acgatgcctg
    68701 caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca catcaaccag
    68761 atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaaggaat ccagcgactc
    68821 cagtttgttc agctgcgtgc ctaattccgc gcgaacggta aggacattat tcagcgagtt
    68881 tttcagtcca cggttggttt tatccaatgc cgcagcggcg atttctttat ccgcttcgct
    68941 atccgcgacc ggcgttttca gggctgcgat ggcgctatcc agcatggcaa aaagattggt
    69001 ttcagaagcg ctaccgtctg gttccgctac cgcgttactg gtaatactgt cgaaaatttt
    69061 gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg acctgttgct taatactttc
    69121 tgttccgcca acataatcac cgttagcctc actaaacggc gcagtctctg ttttatatgt
    69181 agcgcccatt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa
    69241 tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa
    69301 ttttttcctg agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg
    69361 acactttttg agtggcgaaa gtacgtgcca gcgtgtactg gctgttttgc gcctgtgcct
    69421 gggagagaac aacagcttgt gatgcagcaa tgggatcgtc tgaagggtta acgactcgct
    69481 tacccgtcga catctgctcg ccgtacttca tccattctgc ctgagaattg gtgatgccgc
    69541 gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc cttagcgaat
    69601 gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta
    69661 atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga
    69721 ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac tggttttcaa
    69781 cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt
    69841 cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt
    69901 gtcgctatca cccgcatctt cttcgctcgc catcgcgatt ttcgcttcgt cggtgattaa
    69961 tacatccatg ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc
    70021 aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatctgg
    70081 tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa
    70141 cgagattttg taatctgtcg ccagtaccac ggaggcatca gttaccgtgg caccgatcgc
    70201 aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag taaagaaatc
    70261 ttcaccagca tcgccgttag catcaaatcc ggctttgtgt tggctgttga aagcctcggc
    70321 aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa
    70381 tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc
    70441 tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc
    70501 cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcat cgtgatgtta
    70561 taagtaccgc catcctgaac gctgacttct acgccaacaa tctggtttaa ttcgctcacc
    70621 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    70681 tgcgagattt gatcgttcag gctggcaatt tgtttagcgt aattgttgat ctgatcaacg
    70741 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt
    70801 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc cgccgggtct
    70861 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    70921 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcggtc
    70981 aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgt atcatactca
    71041 cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta
    71101 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    71161 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg
    71221 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga
    71281 cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca
    71341 ccttgtcgct tatcgatttc atctgctgga tcatgttggt gagtttacgg gcatagtgag
    71401 gatcggtagc atagcccgcg tcctgtagga cctgcgcccc ctgttccgca ctcgcggcgg
    71461 tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc gacaatgctt
    71521 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccgttttcat
    71581 attcagtcgt ggtgatttca gtgacttgcc ctttccagtt gccagaggct ttgacaccaa
    71641 acaggttata gcttggctcg ccgttttcgc ggcggatttg ccgttgcccc caaccagatt
    71701 caagcgcagc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    71761 gggcgggcag cgagagttgc gcgaggaatg ctctactgtc acccggcagc gaatcatcgt
    71821 agttacgtgg tacggccttt tgcaccagct gcgaaagcgt ctggttttga taacgcacca
    71881 cggtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
    71941 ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct
    72001 gttgggcaat ttgctggtca tacatactgg tatacagacg agtgtgctcg ctgctgaaca
    72061 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
    72121 ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgctcgct ttcgccttta
    72181 gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgctg tcgctgatca
    72241 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcaaaataga
    72301 catcagatcc atcggcgtag cgcccagcgc attgagcgca cgcaccacgt tattgaggct
    72361 ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg
    72421 tggagtaaca acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
    72481 acgattaact gttactgaga gatttccctg cgctaccgcg cagctgtcga gggtcacttc
    72541 gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
    72601 gacattaacc tgcatattct ggatatcggc aaggaaacgg acctgggaac tgttgccact
    72661 tggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac
    72721 gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa
    72781 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
    72841 ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgcctg cggaggctcc
    72901 tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
    72961 aagcggagtc atcaacaacg tacctccacg caagcttttg gcattaccca tggaagaaac
    73021 caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacactg tcaccattac
    73081 cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga
    73141 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccagtgcc
    73201 atccagcccc actaccaagc catagccaat cagtgagttt tgccttaccc cctgaacact
    73261 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa gaattaatgc
    73321 agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc
    73381 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatacgc
    73441 gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
    73501 acgcccgaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt ttcacccacc
    73561 acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
    73621 gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga ggcttcgaca
    73681 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
    73741 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
    73801 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
    73861 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
    73921 ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga gggtatccag
    73981 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
    74041 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttatgcc
    74101 ttagagttgc gtcagttttt gcagcatctg atcggtggtg gacaccgctt tactgttgat
    74161 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
    74221 cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt
    74281 cggtgcaccg gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagtccggt
    74341 atcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
    74401 ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat
    74461 ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt tctgatccac
    74521 ctggaaagag ccgtcacgga tataggctga tgagccatct ggcaacatta cctggaaaaa
    74581 gccctgccct ttaatcgcga catctttgct gttattggtc tgcgacagat tgccctggct
    74641 gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa
    74701 ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc
    74761 aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat
    74821 gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat
    74881 catataatgt cctgtagtgg attgctgaca agcagagcag aagcacgcga cagtgtgaat
    74941 ttcccctcac cctaaccctc tccccaaagg ggcgagggaa tagtccgcgc gcttttattg
    75001 caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta
    75061 tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg
    75121 tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga catcacacgc
    75181 aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc gcttaaacga
    75241 aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag tttcagacgc
    75301 cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat tgtgccatcg
    75361 gcagcaatcg tgatttccgc cccttccggc acagcaattg gccccgcctc gcctatcacc
    75421 ggatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta
    75481 tacccttcgc tgccgtcagc ggtctgcacg gccagccatc ctgctgcaac gcaacgtcca
    75541 gcgggcgaga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg
    75601 ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg cactgcgcgc aaggcgttta
    75661 actgcgcgcg aaaaccgggc gtagaggcat tggccagatt actggcggtt accgcctgtt
    75721 gattcagcgt ctggctggct gctcccatcg cagtataaat tgcgtgatcc attgagctat
    75781 cccgtcagcg attagcgtaa gttaacccgc gtgttgagga tctggtcctg ggttttgatg
    75841 gtctgggcgt tagacttata gttacgctgg gcaacgatca tattgaccag ttctttactg
    75901 agatcgacgt tggacgcttc cagcgcaccg ttggtcagag tgccaaagtt tcccgtcccg
    75961 gctgtcccca acagcgccac gccagaagat tgcgtcgcag accagacgtt gtcgccttcg
    76021 gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgggtt
    76081 tgttcgttgg aattgttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga
    76141 tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt tgttagcgcc ggtattttgc
    76201 tgcatggagt tgaggaagct cagactaaac gtggcgggtt ctgcaccgtt aattgcgccg
    76261 gttgcgatat tattcgccat cgcaccatcc actaatgtgc cattagcatt aaattccagt
    76321 gttgtcgctg tcttcgcaat gctgtttgga tcactgctat cctgggtgta gacctgccag
    76381 ttattatccc cggtcttcac aaaatagacg ctcatgtcat gagcattacc ctgactgtcg
    76441 aaaacagtca ccgaaccttt tttgttatag ctatccgcat tgctggcgct gaatggcgta
    76501 acagtaggaa gcggatcact ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt
    76561 ttcgctgcca tcagggtatt cgggatcgaa atattggtcg gattcgcccc ttgctgaata
    76621 gtcggcggcg taccggttac cgggtaaccc gtcagctgta aaccttgcgt attcaacagg
    76681 ttacggtttt catccagctt aaattgtccg ttacggctgt agaacaccga accgttgctg
    76741 tctaccagac ggaaaaaacc gttctggctg atagcaacgt ccagacctcg cccggtgttg
    76801 gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa cttttacccc cagtcccact
    76861 ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg
    76921 gagttggcga tattgttgcc aataacatcg aggttggtgg cggcagcgtt taatccgcta
    76981 accgcttgag aaaaggccat gactgactcc tgaaatgtga aggcttaaat tatctgccgt
    77041 acttcgtcga gggtggtggt gccgtaagtg ccgagatcca gcgtattacc gctgttgccg
    77101 cggatcacac cctgcaccag agcaaactgc agcggctggg caaccagttg tgtaccaccg
    77161 ttactggcgc taatcgctac attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac
    77221 gaaccgtccc aggtgaaact gtgaactccg gcggtcagtt caccaatatc aatggtgcgc
    77281 acaaccgcgc catttttatc ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc
    77341 tcaacaccaa acggcgtggt cgtggtcaca gccccttctt cactgccggt tcccgcaaga
    77401 acagttgtgc cggggatcat cacgccgtga ccgatcaggt tactggcctg taacgactgg
    77461 ctgttatcaa tctgtccgga aatagatccg agcgtggtat tgagtttttc aatcccgctg
    77521 accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg attggtcggg
    77581 tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg
    77641 ttgctgcccg tgagcgaact actgctggtg gtactgacgc cggtatttgt cggatcggtg
    77701 gtggttaccg caatggacat agctttctcc tttattgacc gagcgtaagg gttttcagca
    77761 tcatgctttt caccgtgttg agcacttcaa cattggcctg atagctgcgt gacgccgaca
    77821 tggtgttaac catctccccg acgacatcaa cgttcggcat ttttatgtag ccctttgcat
    77881 ctgccagcgg attacctggt tcataaacca gtttgtccgg ggcctgactt tcttaacatc
    77941 ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg ttaacctgga ataccacctg
    78001 ttttgcccga tatggctgtc catcaggacc ggtcacgcta tcagcatttg ccagattact
    78061 ggccgccacg ttcaggcgct gggactgggc agttaacgcc gacccggcga tatcaaaaat
    78121 attcagcagt gccatccgtt aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg
    78181 cccgctcaac gcgctaaggc tcatctggta ttgcaggctg ttatcggcaa actgggtgcg
    78241 ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc tggtccggaa tacggtattg
    78301 cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg tgctgcgttg aggtcatcgt
    78361 cagtgcaacc acactggttg catcccgtcc acgttgcatg acttttttaa gttcactggc
    78421 aaaatcgata tcgcgcgcct gataaccagg ggtatcggca ttggcgatgt ttgctgccag
    78481 cacttcctga cgctgggcgc gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc
    78541 gagcttatcg agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct
    78601 ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatgg
    78661 tcattgcgga caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctgacaat
    78721 aaaacgtagc gtggcgatca tcgcgatact gttcagtccg ttaagtacgg cgagcaatct
    78781 cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg ggggtaagtg atgaggttcg
    78841 tgtttctatt cgtacagcgc ccaatctact accgccatgc gagcagccat tgctttcgat
    78901 gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa
    78961 acgatattta caggttaatg tgcaggccac aggaaattat gtggttgccg cgatgcctat
    79021 tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg aaacgtggac gcctggatac
    79081 cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt gatgccgtta gcctgcgcga
    79141 tctatcaccc gatcaaccta tccagttaac ccagtttcgc caggcatggc gggtaaaagc
    79201 gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt agcgccaacg cagaaggtca
    79261 ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg cgcatggtat cggaacaggt
    79321 agtcagcggc gttgttgatg cagatgggaa tattcttata aacctgtaag ctgttaaaga
    79381 ttacccgtcc cttgccgata aataagcaac acatgataaa agcgccctca atgaggaata
    79441 aaccatgagt attgatcgca cttcgcctct gaagcctgta agcaccgttc aaccgcgcga
    79501 aaccaccgac gcgccggtaa cgaacagccg ggcggcaaaa acaaccgcct ccaccagcac
    79561 cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa
    79621 tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac ggtgaactaa aaatggacac
    79681 cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa gacttgcaga gtaactgatc
    79741 gtatgacacg tcttgcagag atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa
    79801 cggtaatgga tcaagagcag caacatctct ctatggggca gatcaacggc agccagttgc
    79861 aatggattac agaacaaaaa agctcactgc tggcgacgct ggattacctc gaacagttac
    79921 gcaagaaaga acccaataca gcaaatagcg ttgatattag tcaacgctgg caggaaatta
    79981 ctgtgaaaac gcagcaacta cgccaaatga atcaacataa cggctggtta ctggaaggac
    80041 agattgagcg caatcaacag gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat
    80101 atggggcgaa cggtcagacc tcaacacccc atcgcggcag taaaaagatt tcgatctgaa
    80161 tattatctcc ggcctgcact gcaggccgga atgcattgtt acaccgtccg gcgggcaaat
    80221 tctttaactt tgaagcccag taccgccagt gcagcgaagt acgcggcaat ccccgccagc
    80281 acgaccgcca ttaaacgcag taagcgccag ggcatggtac ccaatgacca ctccggcatg
    80341 atatgtaaca tacctaaaag cacgccagac atcaccagta ccgccaccac cagacgcaac
    80401 agaaacgcca tccagccggg ttgcggagta aagattttct gcttacgcaa ctgccagtaa
    80461 agcagcgaag cattcagaca cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc
    80521 aacggaccaa taaacgccag gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt
    80581 ttcactggcg ttttaatgtc ctggcgggaa taaaagccag gagccaacac tttcactaca
    80641 atcaggccaa tcaaacccac cgagtaggca attaacgccc gctgggtcat cagcgcatca
    80701 aacgcggtaa atttaccgta ctggaacagc gaaacggtta acggacctga aagaatgccc
    80761 aacgcaaccg cactcggcag tgccaacagg aaacaaagac gcaaccccca gtccatcaaa
    80821 cggttgtatt catcatgatt gccactggca aaacttttcg acagcgacgg cagcaaaatg
    80881 gtgccaagcg ccacgcccag cacaccggag ggaaactcca ttaagcggtc ggcgtaatac
    80941 atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa tggtgttgat gattaaggag
    81001 atctggctca cagagacgcc aaggatcgcc ggtcccatct gtttcaccac gcgcattgct
    81061 ccagcatcgt ggaagttaat gcgcggcagg accagcatgc cgatcttctt caggtgcggt
    81121 agctgataca ccagctgcag aatgccgccg accgtaacag cccacgccag cgccagcacc
    81181 ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt
    81241 gttggcgcaa acgccggaat cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc
    81301 gacgccaggg agatcagcaa gatataggga aaggtaatct ttagtagctg gctggtcagg
    81361 gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg
    81421 gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa
    81481 gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt tatattccgc cagaatcggt
    81541 acaaatgcct gggaaaatgc cccttcggca aagatacggc gtaacaagtt aggaagttta
    81601 aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa agattctggc gacaattgcg
    81661 tctcgtgcga agccaagcac acgcgaaaac atggtcatcg agctgacggc ggccagcgat
    81721 tttaataaat tcatcggtgt tctaatccgg acccacgggc aaaacgcctg caaggcaggc
    81781 gctgaaagag gcgaatagtc tacctagatt atggtgaatt gctaccgcca gatgttacag
    81841 ggtttattca ctcatcgcat cgcgccagat cttgtcaacg atacgttgcg ccagcactgc
    81901 ctattcgccg gcggtttgcg gaactgtctg attttacacg cattcaatga agtgccgcgc
    81961 acagccgaca aatccacgtt gctcaagagt gctctgccaa ccaggaatcg gtttattcac
    82021 tacaccctgc ccgcgctcct cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc
    82081 gtcagtcacg gcctgcacgg tttcacgctg acttccggca cggcgatgca tacaagtggt
    82141 gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt
    82201 agtcagtagc gtaccgccat ccagagaggc tttgccgccc gacaaccaca gcgcggtatc
    82261 caccacatgc agataatcat ccagcaacgt gaaataaaga tcgtgtggcc cgacgctatt
    82321 gctacgatgt ttatccattc ttagcgaggc tgcggtggcg agttgcgttt ttaactcacc
    82381 gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc
    82441 cagttccacc agcctttcag catcgcgcag attttctgcc agcggtttat cgacacagac
    82501 atgtaccccc gcattgagta acgtactgac cacgtcaaag tggctggcgg tgctggagtg
    82561 cacaaaaacc gcatcacaac tggcggcaag gctggataac gaatcggcat aaggaatgcg
    82621 ccagctgtca caaattggca gggctttggc gcgcgtaggc gaccaggctc cttgtaacgt
    82681 ccagtcagac gctgccgcca gcaccggtaa ccacgctttt tgcacaatgc cgcctaatcc
    82741 cactacgccg atacgtaatt ttttcacttt aatctcccag atgggccagc aacgaatcaa
    82801 gacgttgttt cagttctgcc acttcgattt ccagggcttc gacgcgggtc tgtaaatcac
    82861 cgtcaaccat gttcgacata gccgttaccg ccggctgatc ttcaacctca ccactgaaaa
    82921 gatgcatgta gcggctttcg cgtttacccg gttcgcgggc cagacgcacc acaaaaggac
    82981 catcttcgcg attcgccagt tgttccagcg tcgactccac ttccgccata tcgctgaatt
    83041 catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca
    83101 acgtggtgat taacgccact tctgcggcgc tcagtttcag atcgccaaat tctgaattac
    83161 aaaaacgttg ctcatatttg gtgacccgat taccaaaacc gctcactgtg cgtagataat
    83221 gacgtttgac cagattatcc agctgttcct gcacttcgga ttcactcaga ttcatgaccg
    83281 gttcacggtt cgttttctga ttacaggccg tgactacacc attgactgag agcgggtatt
    83341 gctccggcgt ggtcacctgc ttttccagca agcaaccaat tacgcgagct tccagggcag
    83401 taagttgata tttcatgaga ttctccttag cggccgggcg tccagtctgg ggtagttaat
    83461 gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca gatagtcttt cgcatagcct
    83521 tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc gtttattgtg cggcatataa
    83581 ttagccataa tgcgatgaat atgttgggtg cgctgcatat aacgaatggc tgcggtcagg
    83641 gcttcaaaca tgagtccttt gccctgccat ttttgcccaa tcgaataacc gagatagcag
    83701 gcatgaaaag agccgcgaac aacattggaa aaattggcaa cgccaataat ctctttttcg
    83761 tccgggtcga ataagccaaa gtagaaagct gaaccttgtt tatgaaattc gttaatcatc
    83821 cctagcctgg cctgccagcc tgatggataa cagtggcttt cgtcgcgcac tggctcccag
    83881 ggcttgagga aatggcgatt ctctgcgtaa taatccgcca gacgccaggc atcacgatca
    83941 tgcaccagac gcacgaccag tcggtctgtg gttaagcgca cttttggcac gttactgcga
    84001 tagccaaaca ttctatacct actccttccc gtaacgtctt taatacgctg taagtattac
    84061 tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc cattttctgc attattcaca
    84121 tatttgataa aaactctcta tcaaagcgac gttttgataa aaaaatactg tccccgaagt
    84181 gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc tcatctttta tttcccggga
    84241 ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta aatatttcct gctcatcgat
    84301 aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt
    84361 gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc tcggtctacg ccagtttatt
    84421 cagcaaggtc tgggtatttt cggcggcgca attgccgacc gctttggtgc caaaccgatg
    84481 attgttaccg gtatgctgat gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa
    84541 ccgtggctat tgtggttttc atgcctgctc tcgggactcg gcggcacgtt gtttgatccg
    84601 ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac aacgtggtcg ttttttctcg
    84661 ctgttgatga tgcaggacag tgccagtgcg gtcattggcg cattgttggg gagctggctg
    84721 ttgcaatacg actttcgcct ggtctgcgcc acaggggccg ttttgtttgt gctatgtgcg
    84781 gcgttcaatg cgtggttgtt accggcatgg aaactctcca ccgtacgcac gcccgttcgc
    84841 gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca cctatgtcct gacgctggcg
    84901 ggttactaca tgctggctgt acaagtgatg ctgatgctgc caattatggt caacgacgtg
    84961 gctggcgcgc cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg
    85021 ttgctctacc ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttgatg
    85081 gctgggctgt tgataatgtc attaagcatg atgccggtgg gcatggtcag cggcctacaa
    85141 caacttttca ccctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt
    85201 gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg gcagctatat ggggtttagc
    85261 cgtctgggtc tggcgattgg cggcgctatt ggttatatcg gtggtggctg gctgtttgac
    85321 ctgggcaaat cggcgcacca gccagagctt ccgtggatga tgctgggaat tattggcatc
    85381 ttcactttcc ttgcgctggg ttggcagttt agtcagaaac gcgccgcgcg tcgtttgctg
    85441 gaacgcgacg cctgatttga tactcccccc tgactcctcc atactgaaag tgaactgaca
    85501 gccaaatgga ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa
    85561 gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac
    85621 gacgcagaaa cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat
    85681 gacagccgct atatgccaga aagcatggat atcgttcact atgtcgataa actcgacggc
    85741 aaaccgttac tgaccggcaa acgttcccct gccattgaag agtggctgcg caaggtcaat
    85801 ggctacgtca acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct
    85861 actcccgccg cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc
    85921 gacctgctgg cccactctga cggtctgatt aagaatatca gcgatgattt gcgtgcgctg
    85981 gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac tttcggaaga tgatattcag
    86041 ctatttccgc tactgcgtaa tctgacgctg gtagccggaa tcaactggcc aagccgcgtt
    86101 gctaattacc gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg
    86161 atttaatcct gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt
    86221 tctgctggcg agtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga
    86281 tttcatgtcg aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg
    86341 cctgctcgtt ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca
    86401 gtcgcttgcg aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc
    86461 ccatattgtt ctgacaaact tgaccagcca aattggtcgc gaagagccga ataaggttac
    86521 cctaaccgga gacgccaatc tggacatgaa ctccctgttc ggtagtcaga aagcgaccat
    86581 gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa
    86641 agagatggaa gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac
    86701 gttgcttccc tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt
    86761 cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa aaactggcga aaggcattga
    86821 agtgaagcca ggcgaaattg tcattccgtt tacggattaa tgacgagggc gcattcgcgc
    86881 cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat
    86941 cccttcagcc ggagcataga gattaatgac tgcaccatcc caggtattaa agatccgccg
    87001 cccagacgac tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcgtgccgta
    87061 taccagcgaa atttatggac gggctatcgt aatgcccaat ctggctccgc ccgtgaccac
    87121 agttgaggct gccgtggcgt atcgccaacg tattcttgac gccgtacctg ccgggcacga
    87181 tttcacccca ttgatgacct gttatttaac agattcgctg gatcctaatg agctggagcg
    87241 cggatttaat gaagccgtgt tcaccgctgc aaaactttac ccggcaaacg caaccactaa
    87301 ctccagccac ggcgtgacgt caattgacgc aatcatgccg gtacttgagc gcatggaaaa
    87361 aatcggtatg ccgctactgg tgcatggtga agtgacacat gcagatatcg acatttttga
    87421 tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct
    87481 gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa
    87541 tgaacggctg gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct
    87601 ggttggtggc gtgcgtccgc acctgtattg tttacccatc ctcaaacgca atattcacca
    87661 acaggcattg cgtgaactgg tcgccagcgg ttttaatcga gtattcctcg gtacggattc
    87721 tgcgccacat gcacgtcatc gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc
    87781 cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag atgaatgctc tgcagcactt
    87841 tgaagcattc tgttctgtaa acggcccgca gttctatggc ttgccggtca acgacacatt
    87901 catcgaactg gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac
    87961 cctggtgcca ttcctcgccg gggaaacggt acgctggtcc gttaaacaat aaaaatcgta
    88021 gcttcctgtt gtcattaggt tattttacct gtataaataa ccagtatatt caacaggggg
    88081 ctattatgcg aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg
    88141 acgccctggc tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc
    88201 acgtatcggt acgttatgcc gcagcgaata atttatcggt tattggcgca acaaaagaag
    88261 ataaacagcg cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg
    88321 tcagcgaata atatgcagtg atttttgttt ttgccggatc gccccggctt ttttatttta
    88381 cttacgaatt ttgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa
    88441 aaaaatatga atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc
    88501 tgttcccccc tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc
    88561 agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg
    88621 atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc
    88681 gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag
    88741 caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt
    88801 atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc ggtaaacgag
    88861 tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta
    88921 ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga
    88981 gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc
    89041 gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg
    89101 catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc
    89161 gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt gggatgaact ttcccgccac
    89221 aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc ggctgactcc
    89281 gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc
    89341 gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggc
    89401 ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa
    89461 ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc aattcgtcat
    89521 gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa aaaagcgatt
    89581 gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca gcccgtacgt
    89641 aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggcg
    89701 tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg
    89761 ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg
    89821 tttgcggaag tggccagtga tgggtcagaa gccttcccgt tcttgcgcaa tgtattgccg
    89881 ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat
    89941 tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg cctgagtggg
    90001 cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc gcaagaccaa
    90061 aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg
    90121 cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg cactatctca
    90181 tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta
    90241 tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt
    90301 taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt ctacagcact
    90361 caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca ttattaattc
    90421 aaaattattg aaataaaaat aaatatcatt caatttgtta tttttgtgtt gcaattcatt
    90481 ctccgacacg ccattttctt tctctcgcaa attcgtattg gatgcactta tgtcattcac
    90541 aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat
    90601 tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt acgatggctg
    90661 gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt
    90721 tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc
    90781 catgactcgt cttgccgcaa aagctggaca tctcgcattg tgcctattgc tgttcgccat
    90841 cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg
    90901 gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag gcagactttg ccggtgccct
    90961 gcatttttgg ctcgcgtgga gcgtcgtagt actgtctgtt atgcacggat ttatggccct
    91021 gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc
    91081 tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat
    91141 gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc
    91201 ctttgttaac ttccgcatac agcatcttgg ctatagctgg ttatatggca cctttaaaga
    91261 tttcgacggt acttttacct ttgatgaaaa aaatccggct gccgataaag tgaatgtgac
    91321 aattaacacc accagcgtcg atactaacca cgccgaacgc gataaacatc ttcgcagtgc
    91381 ggattttctc aataccacaa aatatccaca ggcaacattc acctccacca gcgtgaagaa
    91441 agacggtgac gaactggata ttaccggcga tctgacgctg aatggcgtaa ccaaacccgt
    91501 cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca tggggcggta aacgtgcagg
    91561 ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat atcaagacag atttaggtcc
    91621 agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga agtaagagtg
    91681 aaatgttgcc ggatggcacg catccggcaa tgatatttat tctgttggat caggaatacc
    91741 cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc
    91801 tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact
    91861 acaacaacct ttgtattttt ccgcgcatac tggacactga ataaacagca ggtggcagcc
    91921 atcattttta cagttggtat ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat
    91981 aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa
    92041 acgcaccggc aagccctgct cgcgcgcctt acgggcgtat tcaataattc cgccctcgat
    92101 atgccagact ttgttgaatc cgttatgttt catccaggca ctggcctttt cacaacgaat
    92161 gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc
    92221 ttttggcagc tgctcacgga aggtatctgc cggaatttcg agcgcgtttt caaagtgccc
    92281 cacttcatac tcatagtggt tacgcatgtc gataaatagt gcatcgggat cgtcaagcat
    92341 ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa agtgaggatc
    92401 gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg cgcagtaccc agaaggattt
    92461 cccgtcatca tccaacgcga tattcaggcg taaaccctct aaagccgggt cgaaggcata
    92521 gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg cgttaatgcc
    92581 ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagct gatataaagc
    92641 gtcacgggtc gccttaggat cggcgatgtg gaaatacttg taaaacgaaa tggtggttcg
    92701 cggttcgctc tcagccaaca ttttggcttt taacgcgtcg ttggaaatgc ggttgtgtaa
    92761 cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg catgatatag
    92821 caattatcga taattaacat ccacatattt tacgctacat ttgcgcatta aaaattattt
    92881 gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg ttgcgggcga
    92941 aaaaatgcga caatacatac aattgcccgt ataggttgaa aaacaggatt gatatgacga
    93001 atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc tggttgggta
    93061 ttggcgtact ttggttggtc gtgcaattgc cctacccgat tatctaccgc ctcggttgtg
    93121 gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg catcgcaacc
    93181 tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg gtgaagaatt
    93241 tcgaatccgt tggcatgggc ctgatggaaa ccggtatggc gtggttctgg ccggaccgcc
    93301 gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg caggcgcaaa
    93361 aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagctgggc gcgcggcagt
    93421 ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca ctgattgact
    93481 ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc aaagatttaa
    93541 aaggcatgat taaagccctg aaaaaaggcg aagtggtctg gtacgcacca gatcatgatt
    93601 acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt tgagcaggct gcgaccacga
    93661 ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc gttccacgcc
    93721 gtaagccaga tggcaaaggg tatcaattga ttatgctgcc accagagtgt tctccgccac
    93781 tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa tgcatcatga
    93841 tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgcccg gaaggcgttc
    93901 cttcacgcta ttaaatctcc catgccggat gcttcagaat ggcatccggc attaccacag
    93961 caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga agtcgggcgc
    94021 ataattagtg tgtttatctt ttcttcttat gttcaccgcg cctggcgcac caacagcgga
    94081 ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac ctgatcgtcg
    94141 cctggctagg ctgttttctt accggggccg ccttcagtct ggtaatgccc ttcttacccc
    94201 tctacgttga gcagcttggt gttaccggcc actccgccct gaatatgtgg tccggtattg
    94261 tcttcagtat aatgggttcc taaacacatc attttaggta ttgaatatga gacgggcaat
    94321 cagctacatc agattttcaa gtgaacgaca actgaaaggg gactctgtac gccgacaatc
    94381 taagctggtt actgactggc ttgataagaa ccctgagttt tacttggatt catccttgag
    94441 ctttaaagat ttggggaaaa gtgcttttag tggtaagcac ctgaaaggtg gtctaggtga
    94501 ctttttaacc gctatcgaga aagggcttgt taaagctggt gatacgctac taatcgagag
    94561 tttagaccgt ctgtcacgtc aggatattga tattgcttct gagctattac gccgtatctt
    94621 aagggctggt gtagatgtag tcacactgag cgatggagaa cattacacac gagaaagttt
    94681 aaaagacccg ttagccctga ttaaaagcat tctgataatg cagcgggcgc atgaggaaag
    94741 tttgagaaag tctgagcgtg tccaagctgc atggaaccgt aaaaaagaac tcatctccga
    94801 aggtatcaag gtatctaggc gttgtcctgc atggttaagg ctgaatgatg accgtcgaac
    94861 attcacaatc attcctgata aagtggaagt tgttaaacga gcatttgacc tgagattaca
    94921 g
//
LOCUS       DAJTNP010000003        75636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTNP010000003 DAJTNP010000000
VERSION     DAJTNP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(437..643)
                     /locus_tag="OO160_000239"
     CDS             complement(437..643)
                     /locus_tag="OO160_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5522508.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="OO160_000240"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="OO160_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5522509.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="OO160_000241"
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="OO160_000241"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5522510.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="OO160_000242"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="OO160_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="OO160_000243"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="OO160_000243"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5522511.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..8042
                     /gene="kdpD"
                     /locus_tag="OO160_000244"
                     /pseudo
     CDS             5362..8042
                     /gene="kdpD"
                     /locus_tag="OO160_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8039..8724
                     /gene="kdpE"
                     /locus_tag="OO160_000245"
                     /pseudo
     CDS             8039..8724
                     /gene="kdpE"
                     /locus_tag="OO160_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8999..9103
                     /locus_tag="OO160_000246"
     CDS             8999..9103
                     /locus_tag="OO160_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5522512.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9414..11611
                     /gene="speF"
                     /locus_tag="OO160_000247"
                     /pseudo
     CDS             9414..11611
                     /gene="speF"
                     /locus_tag="OO160_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11608..12927
                     /gene="potE"
                     /locus_tag="OO160_000248"
     CDS             11608..12927
                     /gene="potE"
                     /locus_tag="OO160_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5522513.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12983..14623)
                     /gene="pgm"
                     /locus_tag="OO160_000249"
     CDS             complement(12983..14623)
                     /gene="pgm"
                     /locus_tag="OO160_000249"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5522514.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14649..15194)
                     /gene="seqA"
                     /locus_tag="OO160_000250"
     CDS             complement(14649..15194)
                     /gene="seqA"
                     /locus_tag="OO160_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5522515.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15379..16143
                     /gene="ybfF"
                     /locus_tag="OO160_000251"
     CDS             15379..16143
                     /gene="ybfF"
                     /locus_tag="OO160_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5522516.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16283..16576
                     /gene="ybfE"
                     /locus_tag="OO160_000252"
     CDS             16283..16576
                     /gene="ybfE"
                     /locus_tag="OO160_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5522517.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16716..17246
                     /gene="fldA"
                     /locus_tag="OO160_000253"
     CDS             16716..17246
                     /gene="fldA"
                     /locus_tag="OO160_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5522518.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17456..17542
                     /gene="uof"
                     /locus_tag="OO160_000254"
     CDS             17456..17542
                     /gene="uof"
                     /locus_tag="OO160_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5522519.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17535..17981
                     /gene="fur"
                     /locus_tag="OO160_000255"
     CDS             17535..17981
                     /gene="fur"
                     /locus_tag="OO160_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5522520.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18065..18391)
                     /locus_tag="OO160_000256"
     CDS             complement(18065..18391)
                     /locus_tag="OO160_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522521.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18428..19834)
                     /gene="chiP"
                     /locus_tag="OO160_000257"
     CDS             complement(18428..19834)
                     /gene="chiP"
                     /locus_tag="OO160_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5522522.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20413..22077)
                     /gene="glnS"
                     /locus_tag="OO160_000258"
     CDS             complement(20413..22077)
                     /gene="glnS"
                     /locus_tag="OO160_000258"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5522523.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDTVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22280..24226)
                     /gene="nagE"
                     /locus_tag="OO160_000259"
     CDS             complement(22280..24226)
                     /gene="nagE"
                     /locus_tag="OO160_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5522524.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24559..25359
                     /gene="nagB"
                     /locus_tag="OO160_000260"
     CDS             24559..25359
                     /gene="nagB"
                     /locus_tag="OO160_000260"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5522525.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25419..26567
                     /gene="nagA"
                     /locus_tag="OO160_000261"
     CDS             25419..26567
                     /gene="nagA"
                     /locus_tag="OO160_000261"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5522526.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26576..27796
                     /gene="nagC"
                     /locus_tag="OO160_000262"
                     /gene_synonym="nagR"
     CDS             26576..27796
                     /gene="nagC"
                     /locus_tag="OO160_000262"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5522527.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27844..28596
                     /gene="nagD"
                     /locus_tag="OO160_000263"
                     /pseudo
     CDS             27844..28596
                     /gene="nagD"
                     /locus_tag="OO160_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28993..30657
                     /gene="asnB"
                     /locus_tag="OO160_000264"
     CDS             28993..30657
                     /gene="asnB"
                     /locus_tag="OO160_000264"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5522528.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31036..31112
                     /locus_tag="OO160_000265"
     tRNA            31036..31112
                     /locus_tag="OO160_000265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31070..31072,aa:Met,seq:cat)
     gene            31121..31205
                     /locus_tag="OO160_000266"
     tRNA            31121..31205
                     /locus_tag="OO160_000266"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31155..31157,aa:Leu,seq:tag)
     gene            31229..31303
                     /locus_tag="OO160_000267"
     tRNA            31229..31303
                     /locus_tag="OO160_000267"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31261..31263,aa:Gln,seq:ttg)
     gene            31338..31412
                     /locus_tag="OO160_000268"
     tRNA            31338..31412
                     /locus_tag="OO160_000268"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31370..31372,aa:Gln,seq:ttg)
     gene            31428..31504
                     /locus_tag="OO160_000269"
     tRNA            31428..31504
                     /locus_tag="OO160_000269"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31462..31464,aa:Met,seq:cat)
     gene            31553..31627
                     /locus_tag="OO160_000270"
     tRNA            31553..31627
                     /locus_tag="OO160_000270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31585..31587,aa:Gln,seq:ctg)
     gene            31665..31739
                     /locus_tag="OO160_000271"
     tRNA            31665..31739
                     /locus_tag="OO160_000271"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31697..31699,aa:Gln,seq:ctg)
     gene            complement(31893..33068)
                     /gene="ubiF"
                     /locus_tag="OO160_000272"
     CDS             complement(31893..33068)
                     /gene="ubiF"
                     /locus_tag="OO160_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5522529.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33214..34638
                     /gene="miaB"
                     /locus_tag="OO160_000273"
     CDS             33214..34638
                     /gene="miaB"
                     /locus_tag="OO160_000273"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5522530.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34791..35831
                     /locus_tag="OO160_000274"
     CDS             34791..35831
                     /locus_tag="OO160_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5522531.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35828..36295
                     /gene="ybeY"
                     /locus_tag="OO160_000275"
     CDS             35828..36295
                     /gene="ybeY"
                     /locus_tag="OO160_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5522532.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36385..37263
                     /gene="corC"
                     /locus_tag="OO160_000276"
     CDS             36385..37263
                     /gene="corC"
                     /locus_tag="OO160_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5522533.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37288..38826
                     /gene="lnt"
                     /locus_tag="OO160_000277"
     CDS             37288..38826
                     /gene="lnt"
                     /locus_tag="OO160_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5522534.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39223..40131
                     /gene="gltI"
                     /locus_tag="OO160_000278"
     CDS             39223..40131
                     /gene="gltI"
                     /locus_tag="OO160_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5522535.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40301..41041
                     /gene="gltJ"
                     /locus_tag="OO160_000279"
     CDS             40301..41041
                     /gene="gltJ"
                     /locus_tag="OO160_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5522536.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41041..41715
                     /gene="gltK"
                     /locus_tag="OO160_000280"
     CDS             41041..41715
                     /gene="gltK"
                     /locus_tag="OO160_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5522537.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41715..42440
                     /gene="gltL"
                     /locus_tag="OO160_000281"
     CDS             41715..42440
                     /gene="gltL"
                     /locus_tag="OO160_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5522538.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42558..43493
                     /gene="rihA"
                     /locus_tag="OO160_000282"
     CDS             42558..43493
                     /gene="rihA"
                     /locus_tag="OO160_000282"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5522539.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43577..45247
                     /gene="hscC"
                     /locus_tag="OO160_000283"
                     /pseudo
     CDS             43577..45247
                     /gene="hscC"
                     /locus_tag="OO160_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45307..46758)
                     /gene="djlC"
                     /locus_tag="OO160_000284"
     CDS             complement(45307..46758)
                     /gene="djlC"
                     /locus_tag="OO160_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5522540.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46755..47462)
                     /locus_tag="OO160_000285"
     CDS             complement(46755..47462)
                     /locus_tag="OO160_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5522541.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47564..48118
                     /locus_tag="OO160_000286"
     CDS             47564..48118
                     /locus_tag="OO160_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5522542.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48128..49555)
                     /locus_tag="OO160_000287"
     CDS             complement(48128..49555)
                     /locus_tag="OO160_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5522543.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49552..50259)
                     /locus_tag="OO160_000288"
     CDS             complement(49552..50259)
                     /locus_tag="OO160_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5522544.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50423..51400
                     /locus_tag="OO160_000289"
     CDS             50423..51400
                     /locus_tag="OO160_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5522545.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51470..51952)
                     /locus_tag="OO160_000290"
     CDS             complement(51470..51952)
                     /locus_tag="OO160_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5522546.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52187..54769
                     /gene="leuS"
                     /locus_tag="OO160_000291"
     CDS             52187..54769
                     /gene="leuS"
                     /locus_tag="OO160_000291"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5522547.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54784..55365
                     /gene="lptE"
                     /locus_tag="OO160_000292"
     CDS             54784..55365
                     /gene="lptE"
                     /locus_tag="OO160_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5522548.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55365..56396
                     /gene="holA"
                     /locus_tag="OO160_000293"
     CDS             55365..56396
                     /gene="holA"
                     /locus_tag="OO160_000293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5522549.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56398..57039
                     /gene="nadD"
                     /locus_tag="OO160_000294"
     CDS             56398..57039
                     /gene="nadD"
                     /locus_tag="OO160_000294"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5522550.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57063..57674
                     /locus_tag="OO160_000295"
     CDS             57063..57674
                     /locus_tag="OO160_000295"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5522551.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57934..58251
                     /gene="rsfS"
                     /locus_tag="OO160_000296"
                     /gene_synonym="rsfA"
     CDS             57934..58251
                     /gene="rsfS"
                     /locus_tag="OO160_000296"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5522552.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58255..58722
                     /gene="rlmH"
                     /locus_tag="OO160_000297"
     CDS             58255..58722
                     /gene="rlmH"
                     /locus_tag="OO160_000297"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5522553.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58753..60654
                     /gene="mrdA"
                     /locus_tag="OO160_000298"
     CDS             58753..60654
                     /gene="mrdA"
                     /locus_tag="OO160_000298"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5522554.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60657..61769
                     /gene="mrdB"
                     /locus_tag="OO160_000299"
                     /gene_synonym="rodA"
     CDS             60657..61769
                     /gene="mrdB"
                     /locus_tag="OO160_000299"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5522555.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61780..62868
                     /gene="rlpA"
                     /locus_tag="OO160_000300"
     CDS             61780..62868
                     /gene="rlpA"
                     /locus_tag="OO160_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5522556.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63007..64218
                     /gene="dacA"
                     /locus_tag="OO160_000301"
     CDS             63007..64218
                     /gene="dacA"
                     /locus_tag="OO160_000301"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5522557.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64329..64592
                     /locus_tag="OO160_000302"
     CDS             64329..64592
                     /locus_tag="OO160_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5522558.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64693..65334
                     /gene="lipB"
                     /locus_tag="OO160_000303"
     CDS             64693..65334
                     /gene="lipB"
                     /locus_tag="OO160_000303"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5522559.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65593..66546
                     /locus_tag="OO160_000304"
     CDS             65593..66546
                     /locus_tag="OO160_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5522560.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66755..67720
                     /gene="lipA"
                     /locus_tag="OO160_000305"
     CDS             66755..67720
                     /gene="lipA"
                     /locus_tag="OO160_000305"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5522561.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67821..68024)
                     /gene="tatE"
                     /locus_tag="OO160_000306"
     CDS             complement(67821..68024)
                     /gene="tatE"
                     /locus_tag="OO160_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5522562.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68153..68941)
                     /locus_tag="OO160_000307"
     CDS             complement(68153..68941)
                     /locus_tag="OO160_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5522563.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69034..69417
                     /gene="crcB"
                     /locus_tag="OO160_000308"
     CDS             69034..69417
                     /gene="crcB"
                     /locus_tag="OO160_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5522564.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69471..69680)
                     /gene="cspE"
                     /locus_tag="OO160_000309"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69471..69680)
                     /gene="cspE"
                     /locus_tag="OO160_000309"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5522565.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69855..70415)
                     /gene="pagP"
                     /locus_tag="OO160_000310"
     CDS             complement(69855..70415)
                     /gene="pagP"
                     /locus_tag="OO160_000310"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5522566.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71004..72389
                     /gene="dcuC"
                     /locus_tag="OO160_000311"
     CDS             71004..72389
                     /gene="dcuC"
                     /locus_tag="OO160_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5522567.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72430..72771)
                     /locus_tag="OO160_000312"
                     /pseudo
     CDS             complement(72430..72771)
                     /locus_tag="OO160_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73109..73618)
                     /locus_tag="OO160_000313"
     CDS             complement(73109..73618)
                     /locus_tag="OO160_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5522568.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73781..74026
                     /locus_tag="OO160_000314"
     CDS             73781..74026
                     /locus_tag="OO160_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5522569.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            74001..75632
                     /locus_tag="OO160_000315"
                     /pseudo
     CDS             74001..75632
                     /locus_tag="OO160_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18617 a        18995 c        19209 g        18815 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc cactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggctatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1621 ggtgcgcagc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat agacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatgc ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgctg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctac ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggctaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcgccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggcggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggctg ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaaagtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcgcggcg gcgcggctga
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggcgcta ttttatggac gctggccttc ggtggttgcc accgtcatta
     6721 atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg
     6781 tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta
     6841 ctgctggtgt gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc acacggcact
     6901 tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca
     6961 gcgaacaatt tattgcctcc acgtttcatg cccgcagtca ggtgttgttg cccgatgaca
     7021 acggtaaatt gcagccgtta acacatccgc aaggaattac gccatgggac gatgccatcg
     7081 cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg ttacccggtg
     7141 taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacgggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ccggaaccgg
     7621 gtttattgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt cgctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggt attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta
     8461 gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc
     8521 ctgctggcgg tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac
     8581 caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat
     8641 ctgcgacaaa aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt
     8701 attgggtatc ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa
     8761 tttattatta aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat
     8821 ataaaacacg aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat
     8881 aattaatgat ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata
     8941 gccatttcat ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat
     9001 ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc
     9061 ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc
     9121 accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg
     9181 tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact
     9241 tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag
     9301 caaactactg atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt
     9361 ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag
     9421 aattaaaaat tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg
     9481 tgaatattga cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg
     9541 ttgaacgtgg aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg
     9601 caacggaaaa tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg
     9661 agcattgcga atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt
     9721 atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca
     9781 atagcgcttt tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag
     9841 ggaatcagtc cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag
     9901 acgtagcaat gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg
     9961 cggcaaaagt gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta
    10021 acaaagtggt tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata
    10081 accacaaatc taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg
    10141 aaacggcacg taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa
    10201 gttacctgcg tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt
    10261 tccgcctcgc tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg
    10321 tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct
    10381 atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga
    10441 acgatccggg tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa
    10501 cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca
    10561 aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg
    10621 ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg
    10681 attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc
    10741 gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa
    10801 ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag
    10861 gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta
    10921 ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact
    10981 tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taacgccatc ctcttcctgc
    11041 tgactccggc agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga
    11101 aaaactgctt gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca
    11161 tgaagagcgc tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta
    11221 tgcccgccac aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca
    11281 cgtcagcatg aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt
    11341 tcgtctaccg gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg
    11401 tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc
    11461 tctggaagaa gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat
    11521 cgaagaacat gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca
    11581 aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc
    11641 gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg
    11701 acaaagcttg ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc
    11761 tcaatggcac cggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt
    11821 atgggcggtt atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc
    11881 tacggcgtct cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc
    11941 accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg
    12001 ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc
    12061 attaccgtgt gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg
    12121 tttagcccga cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca
    12181 gtaggttctt ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg
    12241 aatactgatg tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg
    12301 ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat
    12361 atggagctgg caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca
    12421 gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc
    12481 tggcagttca ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa
    12541 attttctccc gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt
    12601 atccagagcg ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg
    12661 ctggttaacc tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta
    12721 gtcattattc agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt
    12781 gcttttgttg gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg
    12841 ctgtacggtt ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc
    12901 tttgaactga aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc
    12961 gctctttttt tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg
    13021 cttctttctc aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat
    13081 atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca
    13141 gaccgccaat agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac
    13201 ctgccagggt gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg
    13261 cggaagtcgc agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt
    13321 cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac
    13381 acataatgat gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag
    13441 cccctgcgct ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc
    13501 atttgaaacc taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga
    13561 tcatcgcaga agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat
    13621 gctggaacag gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg
    13681 gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat
    13741 cacgcagtgc cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac
    13801 cgtctttatc gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca
    13861 ggttgaggtt gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg
    13921 gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggtca
    13981 gcccttccac gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt
    14041 cgtcgaggga gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccttgt
    14101 cttccaccac tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga
    14161 taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc
    14221 cacctttttt attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat
    14281 tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga
    14341 acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt
    14401 ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc
    14461 tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat
    14521 tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat
    14581 cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct
    14641 gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac
    14701 tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa
    14761 tacggcgtgc ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt
    14821 tcatctgccg caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac
    14881 gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc
    14941 ttttgctctg cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta
    15001 atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc
    15061 accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat
    15121 gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact
    15181 tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta
    15241 tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa
    15301 accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc
    15361 gaaaaaaaga actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac
    15421 aataattctc ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta
    15481 ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt
    15541 ctttcaccga gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc
    15601 ctggatgcac tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg
    15661 gtaatggcac ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc
    15721 gcaccggtcg actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc
    15781 agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat
    15841 gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac
    15901 gtgccggtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg
    15961 gatcaccctg ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt
    16021 gatgatttac tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac
    16081 tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat
    16141 taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg
    16201 actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt
    16261 ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct
    16321 gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca
    16381 gctgcgtatt aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt
    16441 cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa
    16501 tatgagccgt agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag
    16561 tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc
    16621 tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc
    16681 cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg
    16741 gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag
    16801 acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca
    16861 ttctgctgct gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact
    16921 tcttcccgac tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg
    16981 gtgaccagga agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca
    17041 ttgaaccgcg cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag
    17101 catcaaaagg tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc
    17161 agccggaact gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc
    17221 atctcgacga aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat
    17281 taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtaccta
    17341 tacaatgtcc cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt
    17401 attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat
    17461 acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac
    17521 aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa
    17581 cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg
    17641 cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat
    17701 atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg
    17761 gtaaatccgt atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact
    17821 gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg
    17881 caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag
    17941 gcgattgccg cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa
    18001 gccaacctgc gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc
    18061 tcacttattt gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg
    18121 cctgatcatt tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac
    18181 tttcctggtc ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct
    18241 ggcaggcttc aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct
    18301 ctttcagacg gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg
    18361 ccatcatggc gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg
    18421 tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct
    18481 ggaagatgtt accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
    18541 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
    18601 ccaggctgta ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
    18661 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
    18721 ctggaagatt ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt
    18781 tagcgttgaa gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt
    18841 aggttggggt catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc
    18901 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc
    18961 aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat
    19021 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    19081 caaaatattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat
    19141 ttttgaagtc gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt
    19201 tctgataaaa ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt
    19261 aggagaaact caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct
    19321 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa
    19381 tgtaacctgc cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc
    19441 ttataccgct tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg
    19501 cgatttcgtt cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag
    19561 caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc
    19621 aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc
    19681 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg
    19741 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg
    19801 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    19861 tgctgcacca aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa
    19921 gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt
    19981 aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt
    20041 aagtttgcgg ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag
    20101 cggttacatt ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg
    20161 aagcggaatt aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat
    20221 tgtttgctat ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca
    20281 aattcaatat attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag
    20341 cgtttgaaat ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg
    20401 tagaacttaa aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt
    20461 aaataccggt ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc
    20521 aaactggaat gctttacccg caaccgtatc tttcagcgac ggttcagcaa agccctgttt
    20581 gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg
    20641 cacgctgaac agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac
    20701 ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc
    20761 gtcataagta cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc
    20821 cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg
    20881 cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac
    20941 ctgacggctc cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc
    21001 gccctgatag ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc
    21061 attttcgttg agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc
    21121 ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg
    21181 acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc
    21241 ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga
    21301 gaattcgtac tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag
    21361 acgacggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc
    21421 gctgatgcaa tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg
    21481 gtgttcagca aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc
    21541 catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt
    21601 ttcgaacagc gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg
    21661 ctgcgtcagg gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc
    21721 caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga
    21781 gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc
    21841 gacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc
    21901 tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag
    21961 atagccattc ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag
    22021 atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt
    22081 ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg
    22141 acaactgtta gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa
    22201 aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc
    22261 gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa
    22321 catggccctg agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg
    22381 ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt
    22441 gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg
    22501 ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga
    22561 acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt
    22621 tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag
    22681 gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata
    22741 ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag
    22801 ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt
    22861 ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag
    22921 cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca
    22981 gacgggtaat acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat
    23041 agttggttgc cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga
    23101 tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac
    23161 tgaacaccac gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt
    23221 tctggctggc ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag
    23281 agaagccggc atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg
    23341 cgtgcaggag gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca
    23401 caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac
    23461 gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga
    23521 tcgggaagaa gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga
    23581 tgtcgccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga
    23641 tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga
    23701 taccggaacc cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct
    23761 gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg
    23821 cgatcggcac aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg
    23881 accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca
    23941 tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac
    24001 ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga
    24061 agattaatgc gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa
    24121 gtaaatctgg ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga
    24181 gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac
    24241 gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg
    24301 atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca
    24361 tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc
    24421 cttttactgg ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg
    24481 cctgtctgcg caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt
    24541 tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa
    24601 tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg
    24661 tttgtactgg gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa
    24721 atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat
    24781 gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc
    24841 gatcacgttg atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc
    24901 gatgccgagt gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt
    24961 atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct
    25021 tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt
    25081 gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg
    25141 gatgccgaag aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc
    25201 gccgttgaag gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa
    25261 gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat
    25321 ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc
    25381 cttgctcagg gcaatatttt ttaaaatcgg ggggcagaat gtatgcatta acccagggcc
    25441 ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga
    25501 ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg
    25561 gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt
    25621 ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg
    25681 agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga
    25741 aacagggcgt gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc
    25801 tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg
    25861 tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca
    25921 aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg
    25981 ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc
    26041 gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg
    26101 aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg
    26161 atggcctgca tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac
    26221 tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg
    26281 cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg
    26341 gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac
    26401 tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac
    26461 gtctcggcac gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta
    26521 aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac
    26581 accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc
    26641 ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag
    26701 ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat
    26761 caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga
    26821 aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct
    26881 gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca
    26941 gcagacgctg gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg
    27001 caagctgcgc gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag
    27061 cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact
    27121 ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc
    27181 ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg
    27241 cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt
    27301 cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt
    27361 tggctgcctg gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt
    27421 aaagcagggc taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa
    27481 agccgcgaac aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct
    27541 gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg
    27601 tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca
    27661 agcgctgaag gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc
    27721 ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca
    27781 tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag
    27841 tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc
    27901 gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg
    27961 ttgctgacca actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc
    28021 ggtgtcgatg tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg
    28081 cgtcgctagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg
    28141 tacaaagccg gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca
    28201 cgttcctaca actgggacat gattcataaa gcagcctatt tcgtcgctaa cggtgcacgc
    28261 tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg
    28321 tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg
    28381 tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc
    28441 ggcgataacc tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg
    28501 gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg
    28561 atttacccgt cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg
    28621 gttggctctg atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag
    28681 ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat
    28741 ccggcatgaa caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa
    28801 taaaacccgc taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt
    28861 caccaatcga caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca
    28921 gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta
    28981 acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag
    29041 ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc
    29101 ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt
    29161 aacgcggggg cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt
    29221 gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca
    29281 gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac
    29341 gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt
    29401 ggtcgcgacc atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg
    29461 tatgtggcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg
    29521 gcggggagct atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg
    29581 ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg
    29641 gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt
    29701 ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa
    29761 gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg
    29821 ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac
    29881 gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc
    29941 gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag
    30001 atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc
    30061 ggttatcttt acttccataa agcgcccaac gttaaagaac tgcatgaaga gacggtgcgt
    30121 aaactgctgg ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg
    30181 ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt
    30241 aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt
    30301 tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc
    30361 gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag
    30421 caactggaaa ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat
    30481 ctgtaccgcg agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg
    30541 ggcggtcctt ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa
    30601 aaaatggacg atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa
    30661 atgcggtact gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc
    30721 tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg
    30781 taggccagta tttcatagat taatttccca ccactattgt tgaattccga atttcatcct
    30841 gtacgacaaa agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa
    30901 tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa
    30961 aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta
    31021 tcgcgaaaaa aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
    31081 ggtcacaggt tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt
    31141 agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg
    31201 caccattcac cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt
    31261 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg
    31321 gttcaccgcc cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc
    31381 attccctggt tcgaatccag gtaccccagc catcgaagaa ataatctggc tacgtagctc
    31441 agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc
    31501 accaaattct gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat
    31561 cgccaagcgg taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc
    31621 ccagccaatt tattcaagac gcttacatcg taagtgcacc cagttggggt atcgccaagc
    31681 ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac
    31741 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
    31801 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
    31861 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
    31921 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
    31981 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
    32041 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
    32101 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
    32161 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
    32221 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
    32281 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
    32341 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
    32401 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
    32461 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
    32521 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
    32581 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
    32641 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
    32701 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
    32761 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
    32821 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
    32881 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
    32941 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
    33001 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
    33061 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
    33121 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
    33181 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
    33241 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgcctcc
    33301 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
    33361 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
    33421 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
    33481 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
    33541 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
    33601 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
    33661 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
    33721 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
    33781 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
    33841 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
    33901 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
    33961 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
    34021 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
    34081 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
    34141 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
    34201 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
    34261 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
    34321 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
    34381 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
    34441 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
    34501 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
    34561 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
    34621 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
    34681 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
    34741 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
    34801 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
    34861 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
    34921 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
    34981 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
    35041 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
    35101 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
    35161 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
    35221 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
    35281 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
    35341 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
    35401 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
    35461 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
    35521 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
    35581 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
    35641 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
    35701 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
    35761 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
    35821 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
    35881 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
    35941 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
    36001 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
    36061 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
    36121 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
    36181 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
    36241 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
    36301 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
    36361 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
    36421 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
    36481 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
    36541 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
    36601 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
    36661 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
    36721 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
    36781 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
    36841 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
    36901 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
    36961 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
    37021 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
    37081 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
    37141 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
    37201 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
    37261 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
    37321 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
    37381 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
    37441 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
    37501 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
    37561 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
    37621 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
    37681 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
    37741 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
    37801 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
    37861 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
    37921 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
    37981 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
    38041 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
    38101 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
    38161 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
    38221 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
    38281 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
    38341 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
    38401 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
    38461 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
    38521 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
    38581 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
    38641 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
    38701 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
    38761 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
    38821 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
    38881 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
    38941 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
    39001 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
    39061 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
    39121 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
    39181 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
    39241 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
    39301 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
    39361 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
    39421 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
    39481 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
    39541 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
    39601 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
    39661 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
    39721 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
    39781 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
    39841 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
    39901 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
    39961 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
    40021 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
    40081 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
    40141 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
    40201 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
    40261 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
    40321 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
    40381 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
    40441 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
    40501 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
    40561 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
    40621 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
    40681 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
    40741 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
    40801 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
    40861 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
    40921 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
    40981 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
    41041 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
    41101 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
    41161 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
    41221 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
    41281 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
    41341 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
    41401 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
    41461 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
    41521 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
    41581 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
    41641 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
    41701 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    41761 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    41821 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    41881 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    41941 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    42001 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    42061 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    42121 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    42181 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    42241 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    42301 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    42361 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    42421 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    42481 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    42541 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    42601 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    42661 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    42721 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    42781 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    42841 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    42901 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    42961 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    43021 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    43081 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    43141 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    43201 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    43261 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    43321 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    43381 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    43441 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    43501 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    43561 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    43621 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    43681 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    43741 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    43801 aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc
    43861 ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa
    43921 agatgtggtg atctccgttc tggcttattt cagcgatgaa caacgcaagc atacccgttt
    43981 agcagcggac ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc
    44041 gatggcgtat ggcctgcata cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg
    44101 cggcacgttt gacgttacgg tgcttgagta cgccacgccg gtgattgaag ttcacgcctc
    44161 cgctggcgac aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt
    44221 aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt gaactggcag ccttgtacgc
    44281 ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg cacattcgct ggtagtacca
    44341 ggatgaaacg cgggaatgcg aattttacga gaacgaactg gaagatttgt ggttgccgct
    44401 gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag
    44461 tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat
    44521 cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gatccgagca ccattgtcgc
    44581 gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaagtgat
    44641 cctcactgat atttgccctt actcgttggg cgttgaggtt aaccgccagg gcgtttccgg
    44701 cattttctcg ccgattattg aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta
    44761 ttcaaccatg cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca
    44821 caaagttaaa aacaacattc tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc
    44881 ttatcagtcg attgatattc gctttagtta tgatatcaac gggttgcttg aagttgacgt
    44941 gcttctggaa gacggcagcg ttaagtccag agtgattaac cacagcccgg taacattgag
    45001 cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga
    45061 tatgctcatc aatcgcacct ttaaagccaa actggaagag ttgtgggcgc gggcgctggg
    45121 tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa
    45181 cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac
    45241 ctcataatca cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac
    45301 acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt
    45361 taaactgatt ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc
    45421 ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt
    45481 cgtcaacagc atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct
    45541 tgtattaaga cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc
    45601 tacccgatac aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt
    45661 ccaccacgcc catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg
    45721 cgataatttt ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc
    45781 aggaacatat ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat
    45841 cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca acaaattatc
    45901 agcttgtgta gatgggtaac tttcccagta cgcgccgaga tcagcaagca ggctttcccc
    45961 ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc tttctggttc
    46021 ttccgcttgc cactgtttgg ctacaggaat gagttccacc atacctgttt gcaccgagct
    46081 gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggtccttc
    46141 catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa
    46201 gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc taaggaaatc
    46261 gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc
    46321 ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag cacaatttaa
    46381 tgacagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc
    46441 ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg
    46501 atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc
    46561 atctttttct tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc
    46621 ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac acaacggtaa
    46681 gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat
    46741 ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg
    46801 acagttaatg gctgccaggg aagcgtggca tagaaacggt cgcttcccgc tgccagctct
    46861 tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa
    46921 gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata ggcagaaaaa
    46981 tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga ttgcagccat
    47041 aaactctctt cttccgtcag ccagttgttg agcacaccgg cccgtgacag aaatcccatt
    47101 cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg
    47161 aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca
    47221 ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg accatcatca
    47281 gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag
    47341 agatcgaatt cttttatccc ggtatacatt tttggcgagt catgcgcggg tttgcaactc
    47401 tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc
    47461 attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg atgagtataa
    47521 acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata aaaaattaat
    47581 gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac ccaagaaaac
    47641 gatgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat caccaggcag
    47701 tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact caacatcgca
    47761 atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg attatacaca
    47821 ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact
    47881 ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag caattctctg
    47941 gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg gtttgatcta
    48001 cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt taaaactggc
    48061 cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg cacattaacg
    48121 tcgactgtca cccgaagaga tggagtattt tccctatcaa accggcaaag ataaaaatgt
    48181 agaaaataat tttgatgacg cccagacagc ccagcccgcc atcatcaacg ggcattggct
    48241 gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc tgaattaata
    48301 atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg cggctcacaa
    48361 tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg gcaactaaat
    48421 ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
    48481 tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca tcgctgagta
    48541 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt agcggttttc
    48601 cctgatcgtt aaccagatca cgcgcttcaa tcatcatcac cactaatggg taataataga
    48661 cccgatgctg acggcaccat tgcaacatta aagcatccgc ctgggtggag ggatactcgc
    48721 gccagtagta acaaagttct gccagcaggc tttcgccttc tccacaatag acgcgttgtg
    48781 cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac gaccgggcgt
    48841 aatccaggag ttcggggatt cccaggcggc ttgcgctata ccagcggagt aaatttctgt
    48901 gcagttttgc atcatcgggg ataatcactg cgcgatgttg cgctaaatac aatgtcagcc
    48961 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
    49021 gcacggcaac tggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
    49081 catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc
    49141 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
    49201 cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
    49261 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
    49321 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
    49381 atttagcagg cgactgcgca atccgtagcg cctcctcata cgcctgacga agttgtttaa
    49441 aaccctgcgg atcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
    49501 taatatcgac gtcagtcgtt tcctctatat cgaggatctt ccagcaattt ttcataagtc
    49561 gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt gccaggacaa
    49621 ggtagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat tgccggaatc
    49681 gtcgtattct ttacgcgcta agaattcgcg taataaaggc agatgatcat cttcgggaga
    49741 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
    49801 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
    49861 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
    49921 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
    49981 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accagcggtg
    50041 ataaaaccct gccaaccgag cggcatgacc gttctcgatc attcggtgaa ttatgcgcag
    50101 caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa
    50161 atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
    50221 ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc ttaaacattc
    50281 atttaatgaa tgtaaactac atttcgaggc tcagttattt atacttttct gacgcaacat
    50341 aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa tatattttat
    50401 ctacaaccat aaggaaatga taatgatttt tacgtcaagt tgctgcgata atttatcaat
    50461 agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt
    50521 ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct tttactggct
    50581 gaagctggcc gctgagcaag gtcattgtga agcacagtat tccttagggc aaaagtatac
    50641 cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga aaaaagctgc
    50701 cctacaaggc catactttcg cttccaacgc ccttggctgg atactggatc gtggagaaga
    50761 ccccaactat aaagaagcgg ttacctggta tcagatagcc gcggagagcg gaatgtctta
    50821 tgcgcaaaat aatcttggat ggatgtacag aaatggcaac ggagtcgcac aagactatac
    50881 gctggcattt ttctggtaca aacaagctgc attacaaggc catagttacg cacaagacaa
    50941 tctggccgat ctttataaag acggagaagg cgttgctcaa aacaagacac tcgccgcatt
    51001 ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga
    51061 ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact ggtatctgaa
    51121 ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt ataaacacgg
    51181 acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg
    51241 caatgacgcc actgcctggt ataacctggc cattatgtat cactacggag aaggaagacc
    51301 tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga
    51361 tgtcagtcag gaaattcgtg agactgaagc tttactgtag aaataaaaac aagggccggg
    51421 gaatttcatt ctgcccatat tctctttatt gctgtgcgaa atcagactat tacggctcaa
    51481 acgggcgtct ctggaactgg tcataaccac atttcgggca tagcgtcagc acttccggtg
    51541 tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc ccgaccactt
    51601 ctccgctgtg ataaacccca tgatgattga ggtcctggaa aatttcgcgc cattcaagct
    51661 gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa
    51721 agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt
    51781 cacgtctgac agctcgcgtc agctcatcga tctcggttcg cgttaactcc cctgttttta
    51841 ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc
    51901 gttcgttcag tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat
    51961 ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac
    52021 gcccacaaac tctgtaaatt aatgtacgaa taatccgggc atcatttcaa cgtcgagtgc
    52081 gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct
    52141 caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata
    52201 ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga
    52261 agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc
    52321 tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgtta
    52381 ccagcgcatg ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt ttggtctgcc
    52441 tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt acgacaacat
    52501 cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct
    52561 ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa
    52621 aaaaggcctg gtatataaaa agacttctgc ggtcaactgg tgcccgaacg accagaccgt
    52681 actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg
    52741 taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc tgctcaacga
    52801 tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg
    52861 tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt
    52921 ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag ctgcgggtca
    52981 tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta ttgacgaatg
    53041 ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac
    53101 tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt
    53161 cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga
    53221 ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg cggctgacgg
    53281 ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg
    53341 cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc
    53401 gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgcca
    53461 gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccaac
    53521 cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag
    53581 cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg
    53641 tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg
    53701 cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc tgccggtgga
    53761 tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca
    53821 caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg
    53881 tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg
    53941 ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa
    54001 agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa
    54061 caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt
    54121 tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg gtgtggaagg
    54181 ggctaaccgc ttcctgaaac gtgtctggaa actggtgtac gagcacacag caaaaggtga
    54241 tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt
    54301 gcataaaact atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc
    54361 aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg atggcgagca
    54421 ggatcgcgct ctgatgcagg aagcactgca ggccgttgtc cgtatgctca acccgttcac
    54481 cccgcacatc tgcttcacgc tgtggcagga actcaaaggc gaaggcgata tcgacaacgc
    54541 gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca
    54601 ggttaacggt aaagtccgtg ccaaaatcac cgtcccggtg gacgcaacgg aagaacaggt
    54661 tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg
    54721 taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga
    54781 agcgtgcgat atctggcaac actgttgtta tctctggcgg tgttaatcac cgccgggtgt
    54841 ggctggcatc tgcgtgacac cacgcaggtt ccttccacta tgaaggtcat gatcctggac
    54901 tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt
    54961 gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtaaa
    55021 gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag
    55081 atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta cccgattagc
    55141 gccaaagtct tccgttcatt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa
    55201 caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg
    55261 ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat
    55321 actccggcaa cgcctgcacg cgtctccacc atgctgggta actgatgatt cggttgtacc
    55381 cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta
    55441 acgatcctct gttattgcag gaaagccagg acgctgttcg ccaggtagct gcggcacaag
    55501 gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt
    55561 cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag
    55621 aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg
    55681 acgacctgct attgatcgtc cgcggtaata aattaagcaa agcgcaagaa aatgccgcct
    55741 ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc
    55801 agcttccccg ctgggttgct gtgcgcgcaa aacagctcaa cttagaactg gatgacgcgg
    55861 cgaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg
    55921 agcgtttatc gctgctctgg ccagacggca aactgacatt accgcgcgtt gaacaggcgg
    55981 tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa
    56041 gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt
    56101 tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc
    56161 atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga
    56221 tgggcgaggc gttaaatcgc ttaagtcagc cgcagttacg tcaggccgtg caactcctga
    56281 cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag
    56341 ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg
    56401 aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg tcatctaaaa
    56461 cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat ccctaataat
    56521 gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa
    56581 ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc
    56641 ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc ggacgtgccg
    56701 ctggcgttta ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa
    56761 acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg
    56821 gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt
    56881 caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg
    56941 accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg
    57001 gtattgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca
    57061 tgatgcgact gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca
    57121 gcggtcatgc gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata
    57181 cactgctaga tgatgtttcc tttgacttgg ttttatgcag tgaactggaa cgggcacagc
    57241 ataccgcgcg actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca
    57301 acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg
    57361 ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag
    57421 gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc
    57481 actatcagaa tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt
    57541 taattggcat gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg
    57601 ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg
    57661 tcgagaacgc atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc
    57721 cgggcaggct gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat
    57781 tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga
    57841 aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca
    57901 tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt
    57961 gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcaaggc
    58021 aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg
    58081 tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta
    58141 gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc
    58201 atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag
    58261 ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag
    58321 tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggcaaa
    58381 cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg
    58441 gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg
    58501 cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtaag tctactgatt
    58561 ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg ctgagcagag ctggtcgctg
    58621 tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg
    58681 gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag
    58741 aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg
    58801 tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc
    58861 gccaacctgt ataatctgca aattgttcgc tttaccgact accagacccg ctctaatgaa
    58921 aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt
    58981 atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat
    59041 aacgtgcagc aaacgctgga cgctttgcgc agcgtggtag atctgaccga tgacgatatt
    59101 gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact
    59161 aacctaaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc
    59221 gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc
    59281 ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg
    59341 gccaactatg cggcaacgca tgatattggt aagctgggca ttgagcgtta ctatgaagac
    59401 gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc
    59461 cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc
    59521 aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggttgttacc
    59581 gaaccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacctg
    59641 tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc gaatacaccg
    59701 ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg
    59761 gcggtttcgg cattaagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc
    59821 tggtggcaac taccagggtc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg
    59881 cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc
    59941 tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac
    60001 accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag
    60061 aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggccagggt
    60121 tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt
    60181 atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg
    60241 gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa
    60301 gacggtatgt acggtgttgc taaccgccct aatggtacgg cgaataaata ctttgccagc
    60361 gcaccgtaca aaattgcggc gaaatctggt accgctcagg tcttcggtct gaaagcgaac
    60421 gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc
    60481 tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggcggcgcg
    60541 ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct gggtgataac
    60601 aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga
    60661 cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc
    60721 tgattttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg
    60781 atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg
    60841 tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct
    60901 gtattatttt gctggtggcg gtagatgctt tcggagccat ctctaaaggc gctcaacgct
    60961 ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac
    61021 tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aatactggca
    61081 tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa
    61141 catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc
    61201 gtctgattgg cgtcgcagta gtgctggtag cggcgttcat tccgattctg tggttcttcc
    61261 tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac
    61321 tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg
    61381 gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccatactg
    61441 actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg
    61501 ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct
    61561 ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata
    61621 ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg
    61681 gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc
    61741 acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg
    61801 atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg
    61861 gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac
    61921 ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc
    61981 tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat
    62041 gccgaaccag gcagtaacct gacggcctct ggcgaagctt tcgatccgac aaagctgacg
    62101 gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg
    62161 cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg
    62221 ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat
    62281 ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc
    62341 gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt
    62401 tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa
    62461 gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac gaccttagcg
    62521 cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagcttcg
    62581 tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    62641 agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac
    62701 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    62761 tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt
    62821 ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg taaagcaggc
    62881 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    62941 gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt
    63001 cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct
    63061 ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc
    63121 ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa
    63181 gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg
    63241 accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc
    63301 actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg
    63361 ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa
    63421 tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt
    63481 gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg
    63541 gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc
    63601 caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg
    63661 tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc
    63721 gacggcatca aaaccggaca cactgacaaa gcaggttaca atcttgttgc ttctgcgact
    63781 gaaggccaga tgcgcttgat ttctgcggtg atgggtggac gtacttttaa aggccgtgaa
    63841 gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg
    63901 aaagtaggta aagagttcgc ctctgaaccg gtttggttta gtgattctga tcgcgcttcg
    63961 ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa
    64021 gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc
    64081 ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg
    64141 caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc
    64201 catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta
    64261 agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    64321 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    64381 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    64441 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    64501 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg
    64561 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    64621 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    64681 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    64741 gagccaatct cccgggctat gcatgaattc accgataccc gcgataatag tacccttgat
    64801 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    64861 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    64921 tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    64981 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    65041 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    65101 tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    65161 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    65221 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg
    65281 gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat
    65341 acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    65401 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc
    65461 gtaactcacc ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc
    65521 gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca tgaataaaat
    65581 tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca
    65641 gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt
    65701 tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc
    65761 ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt
    65821 attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc
    65881 agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa
    65941 cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat
    66001 cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg
    66061 gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat
    66121 cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcaaggga
    66181 aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt
    66241 ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga gatgtttccg
    66301 gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac
    66361 agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg ttgctggccg
    66421 ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac
    66481 aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca
    66541 ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca
    66601 acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata
    66661 ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct
    66721 ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg
    66781 cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac
    66841 agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc
    66901 tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga
    66961 ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat
    67021 cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt
    67081 agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg
    67141 ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccaacactt
    67201 tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt
    67261 gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa cgcccccaga
    67321 tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac ggcctggcgc
    67381 agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc
    67441 gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta ttgaggtaat
    67501 gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag
    67561 ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc
    67621 cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta
    67681 ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata attacttaca
    67741 tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag
    67801 ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg
    67861 tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg
    67921 gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc
    67981 agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac cttcttgaca
    68041 tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc
    68101 gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata
    68161 attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc acctgacgca
    68221 cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg
    68281 tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg cactcccccg
    68341 ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccagtgat
    68401 gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca gctccctgta
    68461 aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct
    68521 tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca tggcgtgatt
    68581 cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg atgtttcctg
    68641 cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga tgaatcgtca
    68701 gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt
    68761 ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg
    68821 cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac
    68881 aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca gcaaccagca
    68941 ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta
    69001 tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt
    69061 attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt taacccgctg
    69121 catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga
    69181 atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc
    69241 accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt
    69301 ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa ccttctgggg
    69361 tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt acactaaaga
    69421 ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
    69481 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
    69541 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
    69601 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
    69661 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
    69721 tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
    69781 taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa gaagttacta
    69841 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
    69901 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
    69961 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
    70021 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
    70081 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
    70141 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
    70201 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
    70261 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
    70321 gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
    70381 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac atttatcaaa
    70441 aatctactac tagcatagca aagctacaat taacataacc ttaatagacc caacataaag
    70501 aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat ttccgcgcat
    70561 cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc
    70621 ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac gagatacaac
    70681 aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt agattatttt
    70741 ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc
    70801 aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat
    70861 gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc
    70921 atcgccaatt tattgggcat attttttcct taagctttag gaagttttta ttatttactt
    70981 tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg
    71041 ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt gtgttatttg
    71101 tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca
    71161 gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa
    71221 tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca
    71281 tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata
    71341 ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg atgtcactgg
    71401 ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt ccggtgatgg
    71461 taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg gcggcgatta
    71521 ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga
    71581 ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga
    71641 tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt
    71701 tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg ccgttcacgc
    71761 cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta
    71821 ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc
    71881 agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg
    71941 tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta
    72001 ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg
    72061 tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt
    72121 atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt
    72181 tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg
    72241 tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct
    72301 cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg atggtgccag
    72361 gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc
    72421 tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat
    72481 ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg
    72541 gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa
    72601 cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg
    72661 ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat
    72721 actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca gtgtattgat
    72781 tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg atgggattag
    72841 atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa
    72901 agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac actataaatc
    72961 atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg tcaccaaatg
    73021 actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt
    73081 gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct tgtggttcca
    73141 acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa atctcttggc
    73201 gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac
    73261 ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt aatccggaaa
    73321 gcccatattt agccatgttt agctctatct ctcggacttc attttctcct agtttattga
    73381 ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca attttttgca
    73441 tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct tgtgaacgct
    73501 tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca ggcattccat
    73561 ccagttcagt aatttgtttg ggtgtcatcc actggtttat ctcatttgcg cttttcataa
    73621 actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa accacattaa
    73681 ctgatataaa ccggtttggt ttatttggta atatataaaa cagataatca agacgaatta
    73741 tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga
    73801 ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt
    73861 gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca ggccatggcc
    73921 taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag
    73981 tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc atgggtttat
    74041 ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa ggatggctca
    74101 agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct tctcttccta
    74161 acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt cagggatgtt
    74221 ttgaaatcgc ccgccccacg ctggaagctc atgattatga tcgtgaggca ctatggagca
    74281 aatgggataa agccagcgat tcccagcgca gcctggctga aaaatggttg ccctcaattc
    74341 aggccacaga cgaaatgctg aaccagggga tttcaacgaa aacggctttt gcgaccgttg
    74401 cagggcatta ccaggtcagc gcatccactt tgcgggacaa gtattaccag gtacagaagt
    74461 ttgcgaagcc tgactgggcg gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg
    74521 ttcacaaaag tgaatttgac gaggatgcct ggcagtttct gattgcagat tatctgcgac
    74581 cggaaaaacc cgccttccgc aaatgttatg agcgtctgga actggcagcc cgcgagcatg
    74641 gctggagtat tccctcccgt gccacggcct ttcgccggat tcagcaactg gacgaggcaa
    74701 tggttgttgc ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg gcacagcagc
    74761 gaactgtgga acacctggac gccatgcagt ggatcaacgg cgacggttat ctgcataacg
    74821 tctttgtacg ctggtttaac ggtgatgtga tccgtccgaa aacatggttc tggcaggatg
    74881 tgaaaacccg aaaaattctg ggctggcgct gcgatgtgag cgagaacatt gattcaattc
    74941 gcctctcgtt catggatgtt gtgactcgct acagtatccc ggaggatttt cacatcacca
    75001 ttgataacac ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc aatcgctacc
    75061 gctttaaggt aaaagaggac gatccaaaag gactgttttt actgatgggt gcgaaaatgc
    75121 actggacaag cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt
    75181 tcggtgttgg tgggcttgag gaatacgttg ataagcatcc ggcactggct ggcgcatata
    75241 cggggccaaa tccgcaggca aaacctgata actatggcga ccacgctgtt gatgcagagc
    75301 tgtttctgaa aacctttgcc gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa
    75361 cagaaatgtg cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa
    75421 cgattgtgcg taagccaacc gaagaacaaa aacggatgct gttactgcct gccgaggcgg
    75481 tgaacgtttc acgcaaaggc gagtttacgc ttaaagttgg cggctccctt aaaggcgcga
    75541 aaaacgttta ttacaacatg gcattaatga atgccggagt gaaaaaagtt gtggtcagat
    75601 ttgatccaca gcagttacac agcacggttt attgag
//
LOCUS       DAJTNP010000004        72851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTNP010000004 DAJTNP010000000
VERSION     DAJTNP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO160_000316"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO160_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5522570.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO160_000317"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO160_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5522571.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO160_000318"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO160_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5522572.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO160_000319"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO160_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5522573.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO160_000320"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO160_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5522574.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO160_000321"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO160_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5522575.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO160_000322"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO160_000322"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5522576.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO160_000323"
     CDS             complement(4598..5320)
                     /locus_tag="OO160_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5522577.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO160_000324"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO160_000324"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5522578.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO160_000325"
     CDS             6520..6738
                     /locus_tag="OO160_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5522579.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO160_000326"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO160_000326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5522580.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO160_000327"
     CDS             complement(7472..7672)
                     /locus_tag="OO160_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5522581.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO160_000328"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO160_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO160_000329"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO160_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5522582.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO160_000330"
     CDS             10101..12014
                     /locus_tag="OO160_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5522583.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO160_000331"
     CDS             12014..13036
                     /locus_tag="OO160_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5522584.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO160_000332"
     CDS             13030..13248
                     /locus_tag="OO160_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5522585.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO160_000333"
     CDS             13302..14171
                     /locus_tag="OO160_000333"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5522586.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO160_000334"
     CDS             complement(14226..14630)
                     /locus_tag="OO160_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5522587.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO160_000335"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO160_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5522588.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO160_000336"
     CDS             15615..17705
                     /locus_tag="OO160_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5522589.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO160_000337"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO160_000337"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5522590.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO160_000338"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO160_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5522591.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO160_000339"
     CDS             complement(19673..20275)
                     /locus_tag="OO160_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5522592.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO160_000340"
     CDS             complement(20265..20432)
                     /locus_tag="OO160_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5522593.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO160_000341"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO160_000341"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5522594.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO160_000342"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO160_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5522595.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO160_000343"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO160_000343"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5522596.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO160_000344"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO160_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5522597.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO160_000345"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO160_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5522598.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO160_000346"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO160_000346"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5522599.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO160_000347"
     CDS             28269..28436
                     /locus_tag="OO160_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5522600.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO160_000348"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO160_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO160_000349"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO160_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5522601.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHVPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO160_000350"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO160_000350"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5522602.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO160_000351"
                     /pseudo
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO160_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO160_000352"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO160_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5522603.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO160_000353"
     CDS             complement(33764..34849)
                     /locus_tag="OO160_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5522604.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO160_000354"
     CDS             complement(34861..36165)
                     /locus_tag="OO160_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5522605.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO160_000355"
     CDS             complement(36177..36530)
                     /locus_tag="OO160_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5522606.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO160_000356"
     CDS             complement(36541..37419)
                     /locus_tag="OO160_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5522607.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO160_000357"
     CDS             complement(37416..38642)
                     /locus_tag="OO160_000357"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5522608.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO160_000358"
     CDS             complement(38643..39806)
                     /locus_tag="OO160_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5522609.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO160_000359"
     CDS             complement(39890..40252)
                     /locus_tag="OO160_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5522610.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO160_000360"
     CDS             complement(40269..41174)
                     /locus_tag="OO160_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522611.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO160_000361"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO160_000361"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5522612.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO160_000362"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO160_000362"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5522613.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO160_000363"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO160_000363"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5522614.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO160_000364"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO160_000364"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5522615.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO160_000365"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO160_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5522616.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO160_000366"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO160_000366"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5522617.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO160_000367"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO160_000367"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5522618.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO160_000368"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO160_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5522619.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO160_000369"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO160_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO160_000370"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO160_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5522620.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO160_000371"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO160_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5522621.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO160_000372"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO160_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5522622.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO160_000373"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO160_000373"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5522623.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO160_000374"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO160_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5522624.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO160_000375"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO160_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5522625.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO160_000376"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO160_000376"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5522626.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO160_000377"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO160_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5522627.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO160_000378"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO160_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5522628.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO160_000379"
     CDS             complement(59604..61355)
                     /locus_tag="OO160_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5522629.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO160_000380"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO160_000380"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5522630.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO160_000381"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO160_000381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5522631.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO160_000382"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO160_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5522632.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO160_000383"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO160_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5522633.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO160_000384"
     CDS             66301..68622
                     /locus_tag="OO160_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5522634.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO160_000385"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO160_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5522635.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO160_000386"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO160_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5522636.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO160_000387"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO160_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5522637.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..>72851
                     /gene="rpnA"
                     /locus_tag="OO160_000388"
     CDS             72064..>72851
                     /gene="rpnA"
                     /locus_tag="OO160_000388"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5522638.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIA"
BASE COUNT        17554 a        19742 c        18425 g        17130 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatgtccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctag gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag c
//
LOCUS       DAJTNP010000005        72037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTNP010000005 DAJTNP010000000
VERSION     DAJTNP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1322..2908)
                     /locus_tag="OO160_000389"
     CDS             complement(1322..2908)
                     /locus_tag="OO160_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5522639.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3263..5434)
                     /gene="glcB"
                     /locus_tag="OO160_000390"
     CDS             complement(3263..5434)
                     /gene="glcB"
                     /locus_tag="OO160_000390"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5522640.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5456..5860)
                     /locus_tag="OO160_000391"
     CDS             complement(5456..5860)
                     /locus_tag="OO160_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5522641.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5865..7084)
                     /gene="glcF"
                     /locus_tag="OO160_000392"
                     /pseudo
     CDS             complement(5865..7084)
                     /gene="glcF"
                     /locus_tag="OO160_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7095..8147)
                     /gene="glcE"
                     /locus_tag="OO160_000393"
     CDS             complement(7095..8147)
                     /gene="glcE"
                     /locus_tag="OO160_000393"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5522642.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8147..9646)
                     /gene="glcD"
                     /locus_tag="OO160_000394"
                     /pseudo
     CDS             complement(8147..9646)
                     /gene="glcD"
                     /locus_tag="OO160_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9897..10667
                     /gene="glcC"
                     /locus_tag="OO160_000395"
     CDS             9897..10667
                     /gene="glcC"
                     /locus_tag="OO160_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5522643.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10791..11932)
                     /locus_tag="OO160_000396"
                     /pseudo
     CDS             complement(10791..11932)
                     /locus_tag="OO160_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12294..14024
                     /locus_tag="OO160_000397"
     CDS             12294..14024
                     /locus_tag="OO160_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5522644.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14021..14935
                     /locus_tag="OO160_000398"
     CDS             14021..14935
                     /locus_tag="OO160_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5522645.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14968..15216
                     /locus_tag="OO160_000399"
     CDS             14968..15216
                     /locus_tag="OO160_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5522646.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15216..16387
                     /locus_tag="OO160_000400"
                     /pseudo
     CDS             15216..16387
                     /locus_tag="OO160_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16423..17502)
                     /gene="lptG"
                     /locus_tag="OO160_000401"
     CDS             complement(16423..17502)
                     /gene="lptG"
                     /locus_tag="OO160_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5522647.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17499..18569)
                     /gene="lptF"
                     /locus_tag="OO160_000402"
     CDS             complement(17499..18569)
                     /gene="lptF"
                     /locus_tag="OO160_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5522648.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18600..19160)
                     /locus_tag="OO160_000403"
     CDS             complement(18600..19160)
                     /locus_tag="OO160_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5522649.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19172..19996)
                     /locus_tag="OO160_000404"
     CDS             complement(19172..19996)
                     /locus_tag="OO160_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522650.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19996..21345)
                     /locus_tag="OO160_000405"
     CDS             complement(19996..21345)
                     /locus_tag="OO160_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5522651.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21391..22149)
                     /locus_tag="OO160_000406"
     CDS             complement(21391..22149)
                     /locus_tag="OO160_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522652.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22181..22894)
                     /locus_tag="OO160_000407"
     CDS             complement(22181..22894)
                     /locus_tag="OO160_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5522653.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23068..23760
                     /locus_tag="OO160_000408"
     CDS             23068..23760
                     /locus_tag="OO160_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5522654.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23809..25308)
                     /gene="pitB"
                     /locus_tag="OO160_000409"
     CDS             complement(23809..25308)
                     /gene="pitB"
                     /locus_tag="OO160_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5522655.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25600..27459)
                     /gene="gss"
                     /locus_tag="OO160_000410"
     CDS             complement(25600..27459)
                     /gene="gss"
                     /locus_tag="OO160_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5522656.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27664..28530
                     /gene="yghU"
                     /locus_tag="OO160_000411"
     CDS             27664..28530
                     /gene="yghU"
                     /locus_tag="OO160_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5522657.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28653..28901)
                     /gene="hybG"
                     /locus_tag="OO160_000412"
     CDS             complement(28653..28901)
                     /gene="hybG"
                     /locus_tag="OO160_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5522658.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29035..29376)
                     /gene="hypA"
                     /locus_tag="OO160_000413"
     CDS             complement(29035..29376)
                     /gene="hypA"
                     /locus_tag="OO160_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5522659.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29369..29857)
                     /gene="hybE"
                     /locus_tag="OO160_000414"
     CDS             complement(29369..29857)
                     /gene="hybE"
                     /locus_tag="OO160_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5522660.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29850..30344)
                     /locus_tag="OO160_000415"
     CDS             complement(29850..30344)
                     /locus_tag="OO160_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5522661.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30344..32047)
                     /gene="hybC"
                     /locus_tag="OO160_000416"
     CDS             complement(30344..32047)
                     /gene="hybC"
                     /locus_tag="OO160_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5522662.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32044..33222)
                     /gene="hybB"
                     /locus_tag="OO160_000417"
     CDS             complement(32044..33222)
                     /gene="hybB"
                     /locus_tag="OO160_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5522663.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33212..34198)
                     /gene="hybA"
                     /locus_tag="OO160_000418"
     CDS             complement(33212..34198)
                     /gene="hybA"
                     /locus_tag="OO160_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5522664.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34201..35319)
                     /gene="hybO"
                     /locus_tag="OO160_000419"
     CDS             complement(34201..35319)
                     /gene="hybO"
                     /locus_tag="OO160_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5522665.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35508..35795)
                     /locus_tag="OO160_000420"
     CDS             complement(35508..35795)
                     /locus_tag="OO160_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5522666.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35914..36801)
                     /locus_tag="OO160_000421"
     CDS             complement(35914..36801)
                     /locus_tag="OO160_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5522667.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36958..37998
                     /gene="gpr"
                     /locus_tag="OO160_000422"
     CDS             36958..37998
                     /gene="gpr"
                     /locus_tag="OO160_000422"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5522668.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38038..38532)
                     /locus_tag="OO160_000423"
     CDS             complement(38038..38532)
                     /locus_tag="OO160_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5522669.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38723..39607
                     /gene="yghA"
                     /locus_tag="OO160_000424"
     CDS             38723..39607
                     /gene="yghA"
                     /locus_tag="OO160_000424"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5522670.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39879..40304)
                     /gene="exbD"
                     /locus_tag="OO160_000425"
     CDS             complement(39879..40304)
                     /gene="exbD"
                     /locus_tag="OO160_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5522671.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40311..41045)
                     /gene="exbB"
                     /locus_tag="OO160_000426"
     CDS             complement(40311..41045)
                     /gene="exbB"
                     /locus_tag="OO160_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5522672.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41297..42484
                     /gene="metC"
                     /locus_tag="OO160_000427"
     CDS             41297..42484
                     /gene="metC"
                     /locus_tag="OO160_000427"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5522673.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42624..43283
                     /locus_tag="OO160_000428"
     CDS             42624..43283
                     /locus_tag="OO160_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5522674.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43323..44222)
                     /gene="yqhC"
                     /locus_tag="OO160_000429"
     CDS             complement(43323..44222)
                     /gene="yqhC"
                     /locus_tag="OO160_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5522675.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44416..45579
                     /gene="yqhD"
                     /locus_tag="OO160_000430"
     CDS             44416..45579
                     /gene="yqhD"
                     /locus_tag="OO160_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5522676.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45684..46511
                     /gene="dkgA"
                     /locus_tag="OO160_000431"
     CDS             45684..46511
                     /gene="dkgA"
                     /locus_tag="OO160_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5522677.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46711..47637
                     /locus_tag="OO160_000432"
     CDS             46711..47637
                     /locus_tag="OO160_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5522678.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47688..47945
                     /gene="yqhH"
                     /locus_tag="OO160_000433"
     CDS             47688..47945
                     /gene="yqhH"
                     /locus_tag="OO160_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5522679.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47988..50207)
                     /locus_tag="OO160_000434"
     CDS             complement(47988..50207)
                     /locus_tag="OO160_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5522680.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50318..51730)
                     /gene="ftsP"
                     /locus_tag="OO160_000435"
     CDS             complement(50318..51730)
                     /gene="ftsP"
                     /locus_tag="OO160_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5522681.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51805..52542)
                     /gene="plsC"
                     /locus_tag="OO160_000436"
                     /gene_synonym="parF"
     CDS             complement(51805..52542)
                     /gene="plsC"
                     /locus_tag="OO160_000436"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5522682.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52776..55034)
                     /gene="parC"
                     /locus_tag="OO160_000437"
     CDS             complement(52776..55034)
                     /gene="parC"
                     /locus_tag="OO160_000437"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5522683.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55580..56062)
                     /locus_tag="OO160_000438"
     CDS             complement(55580..56062)
                     /locus_tag="OO160_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5522684.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56115..56507)
                     /gene="ygiW"
                     /locus_tag="OO160_000439"
     CDS             complement(56115..56507)
                     /gene="ygiW"
                     /locus_tag="OO160_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5522685.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56659..57318
                     /gene="qseB"
                     /locus_tag="OO160_000440"
     CDS             56659..57318
                     /gene="qseB"
                     /locus_tag="OO160_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5522686.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57315..58589
                     /gene="qseC"
                     /locus_tag="OO160_000441"
     CDS             57315..58589
                     /gene="qseC"
                     /locus_tag="OO160_000441"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5522687.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58635..58967)
                     /locus_tag="OO160_000442"
     CDS             complement(58635..58967)
                     /locus_tag="OO160_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5522688.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59286..59867
                     /gene="mdaB"
                     /locus_tag="OO160_000443"
     CDS             59286..59867
                     /gene="mdaB"
                     /locus_tag="OO160_000443"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5522689.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59898..60212
                     /locus_tag="OO160_000444"
     CDS             59898..60212
                     /locus_tag="OO160_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5522690.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60260..62152)
                     /gene="parE"
                     /locus_tag="OO160_000445"
     CDS             complement(60260..62152)
                     /gene="parE"
                     /locus_tag="OO160_000445"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5522691.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62181..62762)
                     /gene="yqiA"
                     /locus_tag="OO160_000446"
     CDS             complement(62181..62762)
                     /gene="yqiA"
                     /locus_tag="OO160_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5522692.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62762..63589)
                     /gene="cpdA"
                     /locus_tag="OO160_000447"
     CDS             complement(62762..63589)
                     /gene="cpdA"
                     /locus_tag="OO160_000447"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5522693.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63614..64036)
                     /locus_tag="OO160_000448"
     CDS             complement(63614..64036)
                     /locus_tag="OO160_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5522694.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64037..64666)
                     /gene="nudF"
                     /locus_tag="OO160_000449"
     CDS             complement(64037..64666)
                     /gene="nudF"
                     /locus_tag="OO160_000449"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5522695.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64871..66352
                     /gene="tolC"
                     /locus_tag="OO160_000450"
     CDS             64871..66352
                     /gene="tolC"
                     /locus_tag="OO160_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5522696.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66500..67171
                     /locus_tag="OO160_000451"
     CDS             66500..67171
                     /locus_tag="OO160_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5522697.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67177..68337
                     /locus_tag="OO160_000452"
     CDS             67177..68337
                     /locus_tag="OO160_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5522698.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68375..69163)
                     /gene="ygiD"
                     /locus_tag="OO160_000453"
     CDS             complement(68375..69163)
                     /gene="ygiD"
                     /locus_tag="OO160_000453"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5522699.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69306..70079
                     /gene="zupT"
                     /locus_tag="OO160_000454"
     CDS             69306..70079
                     /gene="zupT"
                     /locus_tag="OO160_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5522700.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70137..70307)
                     /locus_tag="OO160_000455"
     CDS             complement(70137..70307)
                     /locus_tag="OO160_000455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522701.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70568..71221)
                     /gene="ribB"
                     /locus_tag="OO160_000456"
     CDS             complement(70568..71221)
                     /gene="ribB"
                     /locus_tag="OO160_000456"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5522702.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71325..71473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71595..71885
                     /gene="ubiK"
                     /locus_tag="OO160_000457"
     CDS             71595..71885
                     /gene="ubiK"
                     /locus_tag="OO160_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5522703.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18021 a        19159 c        17731 g        17126 t
ORIGIN      
        1 aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac agggcttatt
       61 gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata agaaatcaaa
      121 aagccttctc atctatttta agttttccca acggaaaata agagctttca ttaatactga
      181 accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga tccatcggtg
      241 catccctgta tcccgcgatg agactaaaat agtcgtgatg taagctcgta tcagattctt
      301 agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt acattatttg
      361 gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa tctcttacta
      421 ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc aggaaatact
      481 caggtgtcaa cattcgaact ccaactctga aatattcata taagttaatg attttaatga
      541 ttttaatgat tttaatgatt ttaatgattt tttcatttat tcacacctaa taaaagtatt
      601 caaattcaga tgatttttct gctcaacaga cacaaacagg caataatact ctccaaagcg
      661 aacaatattt ggcattacga tttaatgctt cataatcacc aaattattta accataaatt
      721 ttcttatttc taacaaaatg attggtgtgt tgttggagtt aaaatacata cataatatta
      781 acatctgcca acatttcact ttccgcaaga cggatgattt cgaatcctca tccacgttac
      841 cctatcccaa atatctttat taataggcag taacgaacac tgttatagca agattaactt
      901 attaacctaa cggcatcttt tatgtaacaa gtggagataa attgcttaaa attgatcctc
      961 gacaaatctt aaaagttctc attattctgc aaaatcagct ttgcgaataa tcttatgttt
     1021 ttgggagttt tgggaatttt gatctactca ttgttctagt gaaaaatata aataagagaa
     1081 aaaacgccat tcgtcatgaa agcaacagat gaagcaatgt aataacatac taaaattgat
     1141 ctcatgaaag tgggtacttt ataaccagca cagaagccaa gttgtataaa tattaaacaa
     1201 ttcgcatcgt aaaacggaga ctacgtataa agaagccaat cgtaaaaacg gttccctggc
     1261 gagataaacg acagacaaat ggaaagccgg gtgatattta ccttcatccg gctttattga
     1321 tttacgagac taacatcccg gtaaacacat acgcctgcag cagggtgata acgctggcaa
     1381 aaatcagact gtgcttcacg gtgtagcgga acagttcaga ttctcggccc accatgcccg
     1441 tcgcggcgca ggccacggcg atagattgcg gggagatcat cttgccagtt acgccgccgc
     1501 tggtatttgc tgccaccagc agagtgtcag agacgttgat ttgctgcgcc gtggtcgatt
     1561 gcagtgaacc aaacagggcg tttgaagaag tgtccgaccc ggtaaggaat acgcccagcc
     1621 agccgagaaa cggtgagaaa aacgggaaca tcacgcctgt acctgccagt accagcgcca
     1681 gcgtggtgga catgccggaa tagttggtga cgaaggcgaa tgccagcacc atgccaatcg
     1741 acaatatcgg ccacttcaga ctaattagca tttcggcaaa gacgccaatc cctttcttga
     1801 tgcccacgcc gaggatgaag atagagataa tcgccgcaat aaaaatagcg gtgccgccag
     1861 ccgagagagg gtcgaattta aacaccgcat ccattggcgt tggttgggcg acaatgggtg
     1921 ccgctttcaa cacttgttga tgcaaatgag ggatctggaa attaatcacc agtgaataaa
     1981 acgcgccgcc cggagcaaat aacgctttaa acggcttcat ggtccagatg gtgaccagca
     2041 ccgttaagat taaaaacggt gaccacgcac gaatgatttg ccccagacta tattctgaag
     2101 gcacgggacc gccagaagat ggcttattta ccaccatcgc acctgcggat tgccccatgc
     2161 tgattgccgt ttcggtattt ttcggccgcc agactttaag gaataaagcg agtgagacga
     2221 tactcaccag cgccgaagta atatccggca gttccggacc aatatagtta gaggtaaaga
     2281 actgagtgac agcgaagctt cccccagcaa ccagcgccgc tggccacgtc tctttcaccc
     2341 ctttccagcc gtccatcatt gctaccagcc agaacggcac aagaaccgac aggaacggta
     2401 actgacgtcc cgccattgcg ccaatgtgga acggatcgat tcccgttacc tgaccggcga
     2461 ccagaatcgg cacgcccaac gcaccaaacg ccaccgtcgc accaaagcca gcagctcctt
     2521 ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga tcgtcggtga
     2581 tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt aatttataca
     2641 ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata aagccatagc
     2701 ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca atcaggatag
     2761 ataatataag ggttattgct ccagcaacat gtcctttcag acgtaatacc gcgagtgcaa
     2821 cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt cctcccatcg
     2881 gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct ttatatgctt
     2941 gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata ggcgttatac
     3001 tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa gtatatcggg
     3061 taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc atattgttgt
     3121 tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg ataatcgctc
     3181 cggttatttc cgggataaat gtactaccgc agttactatc atagccccga caataaaact
     3241 tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc caggcgtgta
     3301 ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa tcgctggcag
     3361 ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc ggatcgccag
     3421 cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc tgttctttgg
     3481 tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc agcgttgcgc
     3541 ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca atcccctgct
     3601 ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc tcttgttgga
     3661 tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga tcgtccagca
     3721 gcggttcaaa ttcagcactg aactcggtct gggcaatgtt ggcttgcacg ctctgtacgt
     3781 tggtatggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga acccaggctg
     3841 tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc atcaggtccg
     3901 gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag aacagaccgg
     3961 aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc tggtttttac
     4021 gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg tcgaggaaac
     4081 cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctacgc aagttcagcg
     4141 aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtgcc ataccgagca
     4201 ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga ccgtgcattt
     4261 tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt aaatcataga
     4321 gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca ttgccttcgc
     4381 tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac agcaacgagc
     4441 gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg tcattcagtt
     4501 tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc tgcatcaggc
     4561 ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg accgaatctt
     4621 cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg tgcgccggat
     4681 cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg ccgttatttt
     4741 tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac tgtgctggag
     4801 tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta tcgaccacct
     4861 taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca tcgaggaaac
     4921 gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg accatcgccc
     4981 cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc cagcgagcgt
     5041 tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt ggccccgcct
     5101 gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc ggttgcggca
     5161 ccaggtagcc cagttcacga aggaaagatt tataggccgc tttatctttt accggccccg
     5221 gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct gccagcaact
     5281 gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag aatgcagcag
     5341 cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa ttggcgtcaa
     5401 tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt ttcgcttatt
     5461 tcgccaacac cgctgctgcc actttcgcga cctgcgcatc ctgtgctccg gttaaaccag
     5521 aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg ccttccagcg
     5581 acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc tcttcatagc
     5641 ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag atgtaagccg
     5701 caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg tcatcggcaa
     5761 cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg gcaataattg
     5821 cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc cttttcaagg
     5881 gcttgttcta caatttcaat ccagtgacgc acagaggtac gaccggcgct cgccagatgc
     5941 gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc cagcgcattc
     6001 attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata tgttcccgct
     6061 gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag acgaagtaac
     6121 actttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg gcagtggaag
     6181 gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg cagaagttcg
     6241 actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa cgcatcgttt
     6301 ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt ttgcaaaatt
     6361 gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg cgcccgtgcc
     6421 aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc tgcttcgtta
     6481 gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc gttggtgttg
     6541 ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt atggcgcagc
     6601 ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac ctgttccggt
     6661 aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc cggacgcggc
     6721 actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt cactttctgc
     6781 tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga aggacaggtg
     6841 gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt aagcgtgact
     6901 tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc gtccagttca
     6961 tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg aacacaggcg
     7021 cacaggatgc tgtcggcttc cagcgcgcgt tctgccgcat ctcttcggtt aatcgggttt
     7081 gcatagcctg ctcctcaaag ttccgcgtac atgcgaccgg ggttaaacac gccgcaaggg
     7141 tcgagctgct gtttaagctg ctggtgatag cggaataaag gagccgatag cggggcaaag
     7201 ccaccatctc cggcactaaa gcgggtcgca tgaccgccag cgttgcgggc gatgcgatgg
     7261 atttgattgt catcggctgt cgatttcagc cagcgtaacg ccccgcccca gtcgatcagt
     7321 tgctcgccgg gtaaatccat catcggcgca tcgctgggta atgaaatgcg ccataaggta
     7381 cctggtaacg agaagaacgg cagttgttgt tcacgcaatt gctgccagaa ctgacccaca
     7441 acctcttcgc cacccagcag ttcacgcgct gcttttaccg atccttcgcc gccctcaagg
     7501 cggatccaca acgcattgtc gaagtaacat aagccactaa tgggtaatgg ctggagttgc
     7561 cactcggcga tttcactcat ggcttcttgc aggctgattt cccgacgcag gctcagagag
     7621 gcacgcggtc gcggtaacac tttcattgag atttcagtga gcacgccaag acaaccgtag
     7681 cttccggcca ttaaccgtga gagatcgtat ccggcaacgt ttttcatcac ttcgccacca
     7741 aaacgcagat gttttccagt gccggtaatg atgcgcgtgc cgaggacaaa atcgcggacc
     7801 gaaccgctcc acgggcgacg cggccccgcc agcccgcagg cgaccatccc gccccaggtg
     7861 gcttcttcac cataatgcgg cggctcgcag ggaagcattt gcccggcgct ctccagcgcc
     7921 gcttcaatcg ccaccagcgg cgttccggca cgagcggtta tcaccagctc ggtcgggtcg
     7981 taattaacaa tgccgcgatg acaacgcaca tccagcgttt gcccggtgac tgggcgacct
     8041 aaaaaggctt tgctattgct gccctgaatc accagcggcg ttttatcgct aattgcctga
     8101 ttcacctgct ccagcagcgc ctgactgtaa tcacactcgc gtagcatcaa aagcgctcca
     8161 gttcaggaaa aggtaaatga ccgtgatgca catgcatggc accaaattca gcacagcggt
     8221 gtagcgtggg aatgtttttc ccagggttca gcaaaccatc ggggtcaaac gccgccttga
     8281 ccgcatggaa ggtcgtgatt tcatcgctgt tgaactgggc gcacatttga ttgatttttt
     8341 ctcgcccgat gccatgttcc ccactgatgc tgccgccaac ttcaacgcag agttcgagga
     8401 tcttcccgcc cagctcttcc gcgcgggcaa attcaccagg ttcgttggca tcgaaaagga
     8461 ttaacgggtg catgttgcca tctccggcat gaaagacgtt ggcaacacgt aaatcatatt
     8521 gctgcgataa acgggcaatg ccttccagta cgccaggcag ggcgcgacgc gggatggtgc
     8581 catccatgca gtagtaatcc ggggagatac gtcctaccgc cgggaacgca tttttgcgac
     8641 cggcccagaa acgtacgcgc tctgcttcgt cctgtgccaa acggacgtca gtcgcgcccg
     8701 ctttcaacaa gatgtagtta acccgctcgc agtcttcctg tacgtcagac tccacgccgt
     8761 ccagttcgca taacaaaatc gcttcggcgt cgacgggata accggcatga ataaaatctt
     8821 ccgccgcgcg gatcgacagg ttatccatca tctccagccc gccggggata atgccattgg
     8881 cgatgatgtc accaaccgca agtccggctt tttctaccga gtcaaagctg gctaacagaa
     8941 cccgcgccac gggcggcttc ggcagcagtt ttaccgtcac ttcggtggtc acgccgagca
     9001 taccttccga tccggtgaac agcgccagca ggtcaaaacc aggtgaatcc agcgcgtccg
     9061 atcccagcgt cagcgcctcg ccgtccagcg tttgcacttc aattttcagc aggttatgta
     9121 cggtcagacc atatttcagg cagtggacgc caccggcatt ttccgccaca ttgccgccaa
     9181 ttgaacaggc gatttgtgag gaaggatccg gcgcgtaata gagattatgc ggcgcaacgg
     9241 cctaggagat cgccaggtta catacgcccg gctgcacgcg cgcgcggcga ccaacggggt
     9301 taatgtcgag gctctcttta aagcgcgcca tcaccaacaa cacacctttt tccagcggca
     9361 gcgcgccacc agaaagcccg gtgcctgcac cacgggtcac caccggtaca cgcaggcgat
     9421 ggcagacagc cagaatcgct gtcacctgtt ccatttgctt aggcagaaca accagtaatg
     9481 gacgcgtgcg atacgcgctc aacccgtcac actcgtaagg aatgatctcc tcatcggtat
     9541 gcaggatctc aagtccaggg acatgctcac gcagtgccat cagtaccgat gtgcggtcga
     9601 catcgggtaa agcgccatca agacgctctt cgtacaagat gctcatgagt aggcttcgct
     9661 ttgttgtgtt gtgtggcagc tgatttttgc gcgctgcttc tgtgaacagt tattaagcgg
     9721 gcttttcgtt ttcgtctatc tctttagcta ccggtcagac catttttttt ccagctctgt
     9781 gaccttgtct tggttaactt aatgttaaat tgatgtaaca taatcacttg cgtgatgtgc
     9841 gtgttttgcg agttaagaac agaaaaattg gtcctacctg tgcacgaggt ccgggaatga
     9901 aagatgaacg tcgccctatt tgcgaagtgg ttgcagagag tatcgaacgg ttaattatcg
     9961 acggcgtact gaaggtcggt cagccgcttc cctcggaacg tcgactgtgt gaaaagctcg
    10021 gcttctcacg ctccgcactg cgtgaagggc tgaccgtgct gcgcgggcgc gggattattg
    10081 aaacggcgca gggtcgcgat tctcatgtcg cacggcttaa tcgggagcag gacaccagcc
    10141 cgctgatcca tctgttcagt acgcagccgc gaacgctgta cgatctgctc gacgttcgcg
    10201 cattactgga gggcgaatcg gcaaggctgg cggcaacgct gggaacgcag gctgattttg
    10261 ttgtgataac ccgctgttat gaaaaaatgc tcgccgccag tgagaaccac aaagagattt
    10321 cgctgatcga acatgcgcag ttggatcacg ctttccatct cgccatttgt caggcttctc
    10381 acaatcaggt gctggtgttt acgctgcaat cattgaccga tctgatgttt aattcagtgt
    10441 ttgccagcgt aaataatctc taccatcgac cacagcaaaa aaagcagatc gatcgccagc
    10501 atgcgcggat ctacaacgcg gtgttgcagc ggctgccgca cgtcgctcag cgcgcagcgc
    10561 gcgatcatgt acggaccgtg aaaaagaatc tccacgatat cgagctggaa ggccaccatt
    10621 tgattcgctc ggcggtgccg ctggagatga acaaggtggg aatgtgagca gagttttacc
    10681 ctatccccaa ggcgagggga ccgcccctgc acttaacagg atctggtgtt aaatctgttc
    10741 atctataccc tctcccgaat gaccgcccct gcgctaaaca ggatctggta ttaaatttgc
    10801 ttttcataca atcggtaacg cttgtacggc tccgccccaa tgcgttccag catgttattc
    10861 atgcctgtat tggtttcgag gatccatgac atctccagcg catcgatctt ccggcgggca
    10921 aacggatcgc gtaaggcttc aatcaataac agcgcaatca ccgggccgat gcggctgaac
    10981 tgatactcgt cgcgcacgcc catcagcggt actcgcgcag ttcgcacacc gctgactttc
    11041 aaacgccaca gcaattttgc ccagccgaag ggaaagagcg atccgttcag atcggcaatc
    11101 gcctcgttga tgttaggcaa gccacaataa acgcgcaggg tgcagaatca atctcagcga
    11161 tgtagatcat atcgtccggc accagatatt taagttggtc gcccatggtc gcgaattcat
    11221 gttcggtaaa cggcacaaat ccccagttgt gctgccagcc agagttgaaa atctcacgca
    11281 ggatctgcat ctcttcggca aaccgctgac gattgatgca gcgaatggtc acctttttgc
    11341 gcacctgatc catcagtttt tttagcgccg gagagaaagt gagatcggtt cgctgcatcc
    11401 accacgccag taaatcaatg cctttgtgat aacccagttg ttcaatatgc gcggcatacc
    11461 acggtttgcc gtgtggcatc atcgcacagg gtggtgtgtc aaaaccttca atcagtaatc
    11521 cgctttcctg attgatattc aggctgaaag gcccgctgat cttacttgca ccttgtgact
    11581 tcaaccacgc ttccgctgcg ccaaacaacg cggcaaaatc ctgcggatca tcaatggcgt
    11641 caatcatgcc gaaatgaccg gtatcttcac cgtaacgctc acggtgcaag gtatctattt
    11701 gcgcggtaat acgcccaact atctgccccg ctttttttgc cacccacgcc tgccagatga
    11761 tatggtccgt ccccggattt ttcgcagaca aatgctcgtt gcgttcaatg aataaagggg
    11821 gtatccagtt tggatcgtcg ggataaagtg atgacggaaa agcgataaat gccttaaggt
    11881 catttttatt aattactttt tcaattttaa tcatcagcag atcgcattcc atatctctgt
    11941 ggataacaaa taattaatgg tgaaaaacag gttgcaacag caacgagcgg catcttaata
    12001 atcaactcaa tgaatacaaa cgacgaataa atagtattaa ttaatgatta tgataaggtc
    12061 agaggctaca cagaaacgcc tcaatgcctt ttgtcaattt aaggcgaccg gacaaggctt
    12121 cttcttaata tattcataaa ttaaatatca ttcataataa aaacaaaaaa cttaaactca
    12181 ttatttaaat caatgttcat tttcacaccg atgaaaatac tatctcattt atcttttatg
    12241 cctatataat ccaccgcatt attgtcattt tatttttgat atccaatttt ttcgtggtgt
    12301 atatgtctaa taaaatcttt acgcattccc tacctatgcg ctatgccgat tttccaacgc
    12361 tggttgatgc tttggactac gccgctctga gtagagccgg aatgaatttt tatgacagac
    12421 gttgccaact tgaagatcaa ctggaatatc agacattaaa aacgcgtgcc gaagctggtg
    12481 cgaagcggtt gttatcgctg aacctgaaaa aaggcgaacg cgtggcgctg attgccgaaa
    12541 cgagtagcgg gtttgtagag gctttttttg cctgtcagta tgctggctta gtcgccgtcc
    12601 cgttggcgat tccaatgggc gtcggtcagc gggattcctg gagcgccaaa ctccagggtt
    12661 tactggcaag ttgccagccc gcagccatta tcactggtga tgagtggttg ccactggtca
    12721 atgccgcgac gcataacaac cccgaattac atgttttaag ccacgcttgg tttaaggcat
    12781 taccggaagc cgatgttgcg ctccagcgtc cagttccgaa cgatatcgcc tacctccagt
    12841 acacctccgg cagcacccgt tttccccgtg gcgtcattat cacccatcgc gaagtgatgg
    12901 ctaatctacg tgctataagc cacgatggca ttaaattacg ccctagcgac cgctgcgtct
    12961 cctggcagcc tttctaccat gatatgggac tggtcggctt tctcctgccc cccgtcgcca
    13021 cgcaactttc agtagattat ttgcgcactc aggattttgc catgcgtcct ttgcaatggc
    13081 ttaaattgat cagtaaaaat cgcggcaccg tttccgttgc gccgccgttt ggctatgaat
    13141 tgtgccaacg ccgcgtgaat gaaaaagatc tcgctgaact ggatctttcc tgctggcgcg
    13201 tcgctggtat tggtgctgag ccgatctccg cagaacaact ccatcaattc gctgaatgtt
    13261 tccgtcaggt taactttgac aataaaactt tcatgccgtg ctacggactg gcagaaaatg
    13321 cgctggctgt cagcttctct gatgaagcct ccggggttgt ggttaacgaa gtggatcgcg
    13381 acatcctcga atatcagggt aaagccgtcg cgccgggtgc agagacacgc gccgtatcga
    13441 ctttcgtcaa ctgcggcaaa gcgttgccgg aacatggtat tgaaatccgc aatgaagcag
    13501 gtatgccggt cgcggaacgt gtggtaggcc atatttgcat ctccggtccc agtctgatga
    13561 gcggttactt tggcgaccag gtttcgcaag acgagattgc cgcgacgggc tggttagaca
    13621 ccggcgacct cggttatctg ctggacggtt atctgtatgt caccggacgc attaaagatc
    13681 tgattattat tcgtggccgt aatatctggc cgcaggatat tgaatatata gcggaacagg
    13741 aaccggaaat tcattctggc gatgcgattg cttttgttac cgcccaggaa aaaatcattt
    13801 tgcagatcca gtgtcggatc agcgacgaag aacgtcgcgg gcagcttatc cacgcgctgg
    13861 cggcacggat ccaaagcgaa tttggcgtga ccgcggctat cgatctgttg ccgccccaca
    13921 gtattccccg aacgtcctcc ggcaagcctg cccgtgcgga agcgaaaaaa cgttatcaga
    13981 aggcttatgc tgccagtctt aatgtgcagg aatccctggc ataaatcaaa ctgtcgcggt
    14041 gacgggcgct accgggttta tcggtaaata tattatcgat aacctgctcg cccgcggctt
    14101 tcacgttcgc gcattgacgc gtactgcccg cgctcacgtc aatgataatc tcacctgggt
    14161 gcgcggttcg ctggaagata ctcattcact tagcgagctg gttgccggag ccagcgtagt
    14221 ggtccattgc gccgggcaag tgcgcgggca caaagaagag attttcaccc gctgtaacgt
    14281 tgacggcagc ctgcgcctga tgcaagcagc aaaagagagc ggcttttgcc aacgttttct
    14341 gtttatctct tcgctggcgg cgcgccatcc cgagctctcc tggtatgcaa aatccaaata
    14401 cgtcgccgag caacgactgg cagcaatggc ggacgaaatt acgctgggcg tttttcgccc
    14461 gacagccgtg tatggtcccg gcgataaaga gttaaaaccg ctgtttgact ggatgctacg
    14521 cggcctgctg ccacgacttg gtgcgccaga tacacaactc tcttttcttc acgtcaccga
    14581 ttttgcgcaa gcagtgggtc agtggttaag cgccgaaact gtacagacgc aaacctatga
    14641 attatgcgat ggcgtcgccg gtggctatga ctggcaacgc gtacgacagc ttgccgccaa
    14701 agcccgttgt ggttccgtgc gaatggttga cattcctctg ccggtactca caggccttgc
    14761 ggatatcagt accgcgttga gtcgcctggc aggtaaagaa cctatgctga cccgctcgaa
    14821 aattcgtgaa ttaacccacg ccgactggtc ggcaagtaat aaccgtattt ctgaagatat
    14881 caattggttt cccgggatta gcctggaaca cgcattacgc aacgggctat tttgattgtc
    14941 ttgttttaaa ggtgtgttaa cgtttctatg gtaaatcgtg aaatagtaat ggattacatc
    15001 ctttcctgtt tacaggattt ggtcgaaaac ggtgtggaaa ttaagcccga cagcgatctc
    15061 gtcaacgatc ttggcctgga gtcgatcaaa gtgatggatc tcttaatgat gctggaagac
    15121 agattcgata tttccattcc tattaatatt ctgctggatg tcaaaacccc tgcgcaatta
    15181 atggaaactc tacttccatg gctggagaat aaataatggg gctatacgat aaatatgcgc
    15241 gccttgctgg cgagcgtctg caatttagtg acaacgggtt aacaccgttt ggcacctgta
    15301 ttgatgaagt gtattccgcc accgaaggcc ggatcggcaa taaaaaagtg attctggccg
    15361 ggaccaataa ttatctcggt ttaaccttta atcatgatgc catcagcgaa ggtcaggccg
    15421 cgttagcggc ccaaggcacc ggcaccaccg gttctcgtat ggcaaacggc agttatgcgc
    15481 cgcacctggc gttagagaaa gagattgccg agtttttcaa tcgccctacc gctatcgttt
    15541 ttcaaccggt tataccgcta atttaggcgt catcagcgca cttgccgacc ataacgccgt
    15601 ggtgttactg gatgccgaca gccacgccag tatttatgac gtctgttctc tcggtggcgc
    15661 ggaaattatt cgtttccgcc ataacgatgc taaagatctg gaacggcgca tggttcgcct
    15721 cggtgaacgg gcgaaagagg cgattatcat cgttgagggc atctacagca tgctcggtga
    15781 cgttgcgccg ctggcggaaa ttgttgatat caaacgtcgt ctcggtggtt atttgattgt
    15841 cgatgaagcg cattcgtttg gcgtgttagg cgcaacgggg cgcggactgg ctgaagctgt
    15901 cggcgttgaa gatgatgtgg atattatcgt tggtactttc agtaaaagtc tggcctcgat
    15961 tggcggtttt gctgtcgttt cggaagcgat ggaagtgctg cgctacggca gccgcccgta
    16021 tatttttacc gcctctcctt ctccgtcctg cattgcgacc gtgcgctcgt cgttaagaac
    16081 tattgccaca caaccagagc tgcggcaaaa gctgatggat aacgccaacc atctgtatga
    16141 cggtctgcaa aaactgggtt atgagttaag ttcacatatt agcccggtag tgccagtaat
    16201 aatcggttcg aaagaggaag gtttacgcat ctggcgcgag cttatctcgc cgggcgtgta
    16261 tgtgaatctt atcctgccgc ctgccgcgcc tgcaggcatc acgctgctgc gctgtagcgt
    16321 aaatgctgcc cacagtcgtg agcaaatcga tgccatcatt caggcatttg ctactctgaa
    16381 gcagtaatgt gagcccgccg tggtgtttgc gccacggcgg gtttaatgtt gtctgtcata
    16441 ccaatacacc aggctcaacg ccaccaacac aaacgctacc ggtaaaccga gcgcggtaac
    16501 aggtgcgctt aacgcaaata gcaatcccag attaaccatg atttgatagc tgatccaggt
    16561 gagtaagccc acgatgacgc ctacagcgag acggctaccc atcccaggcg aacgcggggc
    16621 actaaaggta aacggcacag ccagcaaaat catcgccagg gtcaggatgg gttgccccag
    16681 tttttcccac agtgcaaggt ggtattcgct gctgggttgc ccggtttcct gcaaataatg
    16741 gatgtaatga ttaagctgac ggacagaaaa agtattcccc ggcattgata actcttcaag
    16801 attcatactg gtgaaggccg attgccaggc aagattatct aatgtttcca gcgtttcttt
    16861 gccattcagc cattttttat gattcacacc atgcaggacc cacgttttgt cgtcttcaat
    16921 ggtggcactg cgtgcataaa tgtaggattc aagcgagaga tcgtcgcgat aatgaaatat
    16981 ctccacgccc accggctggc cctgctcgtt cagcgatttc accgtcacaa attcattacc
    17041 acgcctggcc cacagcatat tgccggtaat gtcatcgtcc tcccccaacg cggtggcggt
    17101 cgatttgatt tgcaacgcct gttgctgcaa tggcgacgcc acccactcat ctatcgcgcc
    17161 taacgaaaca gtccacaata tcccggcaac cagcgccacc agtgcaatac ggaagataga
    17221 aaaccccgtg ctgcgaatgg cggtaagctc actgtttttc gataactgcc ccagtccaac
    17281 aataccaccc aataacgcga taaacggcga aagttcgacc agtgtgcgcg gtaaggtcat
    17341 tagcaccacc agcaccgcct gagtccagcg ataaccgccc gggctgacac catccagttc
    17401 gttaatcagg ttgaaggtgg taaaaagcgg cagcaatagc cctgcagcgg cggcaaaacc
    17461 gagaaagaga tgacggatta aatagcgact gaaaacattc atcgggaaaa tttctgcagc
    17521 aaagagaagt cacgtgcgac cagtatcagc aggcccatta gcatgagtcc gggcactaac
    17581 cacaaaccag gaatgagggg aatcgcgccg ttagccacca gtgtacggca gacgtcgccg
    17641 ccataaaaaa tggcaataaa cagcaacgtc agtggcaata acgttgaaaa tcgcccttgc
    17701 cgcggtttta cccgacttaa tgaaatggcc agcagcgcca tcaacaatgc ggtcaggcca
    17761 cggctttgtc gccattgcag ttcggcatga tcggcaggaa aggcggagcg cgccagctcc
    17821 gtgaccgatt tcgctttgcg tttgacgttc gggctttgat ccagcggatt cagatgtaat
    17881 tgcagattac ggtagatctg ttcgttgtcg tcacgcccct gatgatcgag aagataggca
    17941 gtgccgttgt gcaacataac ggtcggtttc tcgggtgatg ggtcaaccac atcaaccgaa
    18001 ccggcgcgga aaatacgggt tcgattggca gtagaagtat aaatcagcgc gtcagtcagg
    18061 cgattattat cctgatcaac cgtctgcgaa aggatcattc gtccgttatc gttagtgtta
    18121 aatttctttg cccgcaactg gcgaacatcc agctccgact gcgactgctg ctccagttga
    18181 taaatctgcg cataagccca cggacgacca tacatcgaca gaagggtgac aaaaatactt
    18241 agcggtatcg ccagataaag aacggctttg tacaagcgtc ccggactgcc ccccgccgca
    18301 gagatagcgg taatttccga gtcggtatac atctgcccta gcgtcacgcc aactgacaca
    18361 tacagaccaa caggtaacaa catctccagt gcaatcagta ctttgtagaa aacgatatcc
    18421 agcacaacat caagagctaa tgtcccattt gccgcttcgg tcagataacg ctgtgcggag
    18481 tagctggcga aaataaagat cagaaaaccg acgataatca gcaccagacg gcgcgtacca
    18541 cgcatgatgt agtgttcaac cagcttcatc tcactaacgc cctcaatcct ttgagttttt
    18601 tattcattga tcagtttctg cgccaacgca atgacatgat ctacctcttc cggcgtcagg
    18661 ctgccgtcct ttgcccactg aatttgcccg gctttattca gcaccagaat ggttgaactc
    18721 tgctccggca agcgccaggc aacgcgtccc tgtccattgc cgtcaataac gaattgcgcc
    18781 cacggatagc ggcgtttatt tttttcgatt ttgccaacaa caaaatagcc agtaccaaag
    18841 atggcatcat cggtgttcac aatggtggtg ggctgaaaac gatcctgcgg aaagtttgcc
    18901 gcttcaaccg ctttaattaa cagtgagttc tttttcttcg ccgacttacg cccggcgata
    18961 tactgaataa tgcggacttt tcccgctaat tgtgtgctgt tccacttttg gtagtgaatt
    19021 tcgtcgtcgc tattcagaag cagctcgccg ccttcctgga tataaacagg ggtaaccgtt
    19081 ttgccagtga cgaaattgtg tgcctgcaca cccggggcta agagcatgat aatcaacgca
    19141 ataatcaaac gagatgacat cattgctccg gctatagcgg aaaacgcgaa atcagcagct
    19201 ctaagcgctg acaaatatcg ctatccagcg ccaacggtgc ataatacgac tggcggtttt
    19261 ccatatcatc cgccaccatg tcgtcgtgtt tatctcccat caggccatta atggcggcga
    19321 ggaaacggga aatctgtaac gcctctttat tttcaggatc aagcgatctt aaggtgtcgt
    19381 aataagaaac ctgctggctg atgatttttc gcacatcatg aaattccctg ccggttagtc
    19441 gcggtttggc aataatctct ttcagggtat gcatttgctt ttgccgatag gcaataaaac
    19501 tgcttacgct ttctaactgg ctatggcgta atgaatagtt caccaacgac cagaccggag
    19561 agcttaaata aatccgtaac aggttgccgt aatacgacac aatatttttc ttaccgttac
    19621 gaaagccgtc ctgaaaacgc cccagaaaaa cggtcgtttt gccgaataaa aatccggcct
    19681 gatgcttctt taaatacagc cgttgcgcaa aacgcaggtg cttgtagcgc gctcgcatgt
    19741 atttaaattg tttgcgctca tcagcagtca gctcatcgcc tttggcttgt ttgcgaacta
    19801 aacgcagtaa ttcctgacga tcaacgccat tgagccagaa ttgcaggcat aacgcgtaat
    19861 tatcatgaat gatttcggtg gtacaactta gtgcaatagt cggcggcaaa ctaacctgtg
    19921 catcaacgac atcatcttct tcaatcgcaa caaatagacg ctcgctcgcc tcaagcggga
    19981 aatattttct catcatcagg atttctctcc ggctgcgtta taaagcaagg aatggcgctg
    20041 aaaaaatgcc tttaaggttg gaattaacga gagcgccacg tccagacatt gcccaccgaa
    20101 gtaagcaaac gctgctccca gcacaccaaa cagatgacag aacatgaaca gcaatacgat
    20161 gtagccgatt gaggcgatgg tttgcgccac gagaaaagcg cgctgttttc ccgccatcaa
    20221 tagcaatgac tcctgcggga agcccagcat cgaaatcaca attgcgccca gcatcacctg
    20281 aatcaggtca tacgcttcga gatacttaac gccaaacacc agcgaaatga gcggcttgcc
    20341 gacgatcaac accgcgagcg ccaccagaat accgattccg cccgccagta aaccggactt
    20401 cacacctaac aaccacggtc tggtggtgcg cggatctaaa cgcatcacct ctgggtaaaa
    20461 acttttaccc agcaaacccg ctggcgttcc ggcggcgtcg aagaatgtca tggcgatttt
    20521 aaataacccg gcggcagcgg gtcctaacac gatccccacc aacactgtgc tgcacgagtt
    20581 acgcgccgac cagatggagt gggcaatgtt ggttgaccag acaaaactcc acgcgccttt
    20641 aatgtgtcgg gcagactcaa acagattcaa tttgaaggcg ttatggatat ttctacggcg
    20701 taattcgcgc gcggcaaacc accagtacat ggtgccgcca accagattcg acacgtacca
    20761 ggcaataaca aaacccacaa aaccaaagtc aaaataccag gctgcgacgc tccccgctgc
    20821 gcgcagaaaa ggtttcgtcg cctgctgtac agcaatcaaa tcaaagcgat ctaccgcacg
    20881 cagaatgccg gtcggcgtgg aggaagccat tgaaggaatg agcgtgcaat acagcgccgc
    20941 cagccaaaag ctttggtcat ccagacctaa tgaatgggaa aggaatggca gtaaggcaat
    21001 gccgccgaca atcgccaccg cgccgctgac gatatccagc gagaaggaaa atgagacgac
    21061 attgcgaaat tgctgcggat tattgttggt caaggctggc gttccgtact gaaccaccag
    21121 ctgccatgtc tgaaacttaa taaaatcgct gatcgacttg gcgtacgatt gcacaatcac
    21181 cagtacacca aacatggcgg gcgtcatccc tttaccggca cacgagagcg ccaacagccc
    21241 cagcaaggcg ctctcgacat tactggagcc taaccaggcg ctattgcgaa taatggtgcg
    21301 aaacgcgcca tctgcaaacc aatgtttgat gttaaaaccc gccaattcag cctgacctta
    21361 tcgttgatag taaaaaatat cccgccagcc ttaagttaaa cttcggcggt cagaaacgat
    21421 ggcaaccaga gaaaccgcct tctgtacctg ttccagcact tcgctgtagg gcgctctgga
    21481 atcaatctca agaatttttg tgccgttata gccaatcgtc gacatgacgc cgattttgtc
    21541 ctgcagctcg gcatactcat ggtcaggctt gcgggagatg gcagtatcaa tatcaatccc
    21601 caaacgaatg attaattcag gacggtattg cgccattttt tggtataaac tccgttcgcg
    21661 ctgcgccaga aacatgctga ttttcccggt cgcacgttcg acgccgatcc ccggtccgtc
    21721 ataataaaag cccgaaattt cagcctgcgg gaagcgatcg ctgaccacca gaacgccact
    21781 ttgcgccagt cgctgaacct tgcgtagatt cgccattctt cgcagcgaga agcagtacat
    21841 aatcaccgcc gcccatagcg ccggagattt ggttttcatg ctttgggttt tcgaggattt
    21901 ggccgccaat cgccgttcca gccagacgcc aaccaacggc aatcgtttga ttttgtcgcc
    21961 gtcttcgccg gagagcagcc ccagatagcg gcgctcggtt tgccagtgtt gttgcagcga
    22021 tttcaccagg tcggtggtca gcgtggattt accggtgcca tcacacccga ccaccgcaat
    22081 cagccccgga atgtagttgg gctgcggcgc ggttgtacta ttaacagttt gtgtttgtag
    22141 tgcatccatt cgttaaatca tcccctatcc attttggtga tcaggaaagc gcggcgtgaa
    22201 ttgcgcgtag tgacgcttgc ataatttcat cggctggttg ccgaccatcc agttcaagga
    22261 tcttggcgcc attgaatgtc agttgcggcg taacggcgat tttttcctgt aacgctgcca
    22321 gttggtggtc aggtttacgc gcaaacgcgg tttgttcatc aatgccaaga cgaatcaaca
    22381 atacgggtaa ataagacgcc atccattggt acagcttcaa ctcgcgctgc cttaacattt
    22441 ttatccaacc gttaccgccc gtggtttttg ccaattgcgg gccatcaaag cgaaaacccg
    22501 gcacttcaac ctgcgggtag cggtcggtga tgagcagata gccttgctgg cttttacaca
    22561 acattttgcg aaacttgtac gcccgccagc aggaaagcag atagataacc agcgcagtaa
    22621 tattgccagg cggagttaag ggcttttcgt gtacatgtgc cgctttactt cgcagataac
    22681 acccacaagg tgcgccaata acagggagct gtgaaatcca ttcgccaatt cgcccggacg
    22741 attgcccgag ataaatgtgt tctgttggca ttcttgctgc cagttcattt accaggcttg
    22801 ccgtgagggt cgatttacct gaaccatcac atcctacaat ggcaataaca cgcactggcg
    22861 tggaattaat tattgacata ttacgttgat tcacgaaaaa cccggcagta aataatgtat
    22921 tgaatattac gtggtccgtg ctcagaatat ccgttcaacc ttattgtgac aatgactaat
    22981 tgtgacattg acctgaatta cgctttcata aaaacatatt aaccaaataa atatttttaa
    23041 tggatattta aattaaagga tatattcatg cagtcaataa cacctccatt aattgccgtt
    23101 attggtagcg atgcttcagg caagtcaacg gtgtgtgaac atcttattac cgttgtcgaa
    23161 aaatatggtg ctgccgaaag agttcattta ggaaaacagg ccggaaatgt cggtcgtgca
    23221 gtgacaaaat tacctttgat gggaaaatcc ttacataaaa caattgaacg aaatcaggtg
    23281 aaaacagcaa aaaaattgcc tggaccagtt ccggcgctgg taattacagc gtttgtcgcc
    23341 cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc gtcgcgggtt aattgttcta
    23401 accgaccgtt atcctcagga ccaaattcct ggcgcttacg atggtacggt gttcccacct
    23461 aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc aggaacgtaa agcgtttcac
    23521 tggatggcga gccataagcc tgatctggtc atcaaactca atgttgacct tgaagttgcc
    23581 tgtgcacgta aacccgacca taaacgggaa tcgctggcga ggaagattgc cataacgcca
    23641 cagttaacct ttggtggtgc acaactggtt gatatcgatg ccaatcagcc actggaacag
    23701 gtgttggttg atgcagaaaa agcgattacg gattttatga ccgcgcgtgg ttatcactag
    23761 tcaaaaatgg aaatgcccga tcgccaggac cgggcatttt caggaaggtt aaatcaactg
    23821 caatgctatc cagtacagcc caccagaaag aaaaattgcc gccggtaaag taaacaccca
    23881 cgccatcagg atgctggtta ccgttttacg ctgtaaccca ccgccgtcca ccaccatcgt
    23941 ccctgctacc gcagacgaga ggacgtgtgt tgtggagaca ggcatcccaa tataactggc
    24001 aaggccgata gacactgccg ccgtcatttg tgccgccatg ccttgcgcat acgtcatgcc
    24061 gcgcttacca atcttctcac cgatggtcat cgctacacga cgccagccaa tcatggtgcc
    24121 aatgccgagc gccagtgcta ccgccatgat gatccacacc ggagcgtact caatggtgct
    24181 taacatatcg ctgcgcagtt ttttcagcag gttctggtct tctttactga cgcctggcag
    24241 tttcgccagc ttcgcggagg tatcggagat gcacagcata atgcggcgca gctggctgcg
    24301 ctgactcacg cttaacggct cgtaactttc catattgcct ggcagcatcg ttttaacgcg
    24361 cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac tctgttactt gcgtgccatc
    24421 agtcgatgct gcaggcaatg gaggttccat cgcaatcaac ttctgcggca gttcaggatg
    24481 ctgttgcagg tagtgttcga agttggtaac ggcatcgcgg gtacgggtaa tttcatagcc
    24541 ggacgcattc atattgacga cgaagccagc aggggcaatc cccaccagta ccagcattac
    24601 caggccgatc cctttctgcc cgtcgttcgc gccgtgcgaa aacgccacgc ccgcagcgga
    24661 aacaatcagc gcaatacgcg tccagaatgg cggtttacgt ttgccctttt tctttttgcg
    24721 atcttccgga atgcggtgaa tacggtcacg ctttttcgtc ccactccaga aacggcgcag
    24781 caggaaaatc agaccacccg caatgaccag gccgacgata ggggaaacaa tcagcgagga
    24841 gaaaattttg gttacttcac gcaggtttaa cgcatccatc accgatgagc cggttaacag
    24901 cgcgttggtt aaaccgatgc cgataatcgc accaatcaag gtgtgcgaac tggaggccgg
    24961 taaaccaaag aaccacgttc ccaggttcca gataatcgcc gccagcagca tggaaaagac
    25021 catcgccaaa ccgtgagttg accccatatt cagcaacaaa tcgattggta acatatggac
    25081 aatggcatag gcaacgctaa gtccgcccaa taacacgcca aaaaagttaa aaaatgccgc
    25141 catcaccaca gcaagttgtg gctgcatggc acgagtataa ataacggttg ccaccgcatt
    25201 cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac acaaatgcca gagcaagtaa
    25261 taacaaaagc cccgtatata tatcaaggcc aacaaataaa tttagcatat attagattac
    25321 gccattttga atttacgaac ggacgcatta tcagtgactt taacggcatg ggcaaagtga
    25381 aatatcattt tttaacgata aaaagagtgt tatttcttgt gtatcagaca ataagcattc
    25441 attcaaaacc tttagaaata atcaaaacac aggtattcca ctggttaata tattcacata
    25501 tgaaatgaat aaatattgga atatataaat attgaatatt ttgattaatc cccgtactaa
    25561 ttattcttca taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa
    25621 cggttcaata tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc
    25681 accgtagttg ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg
    25741 caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc
    25801 cagcacctct tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcgatcgg
    25861 tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag
    25921 gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat
    25981 caccgtccac agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg
    26041 cacttcgttt tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac
    26101 ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag
    26161 tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg
    26221 caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata
    26281 gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt
    26341 ccaggcacca gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata
    26401 gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc
    26461 ggcgttgtat tcgtaaacct tcaggccacg ctcatccatg cagaaatcca tacgaccagt
    26521 gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg
    26581 aatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata
    26641 catcagatgc agctcgttgg tggctttaat tagctcctgc tcggcactct cggtaatggt
    26701 gtagtaatga tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg
    26761 tagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc
    26821 tccactgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc
    26881 ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata
    26941 gcagccgttt tcgaccacca tctccagctc gcgcgtccac tgttgccctt gcggcaacgg
    27001 ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat
    27061 ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc
    27121 gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac
    27181 cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac
    27241 cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc acttgtggcc
    27301 catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc
    27361 gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt
    27421 cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca
    27481 acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac
    27541 agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca
    27601 cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc
    27661 actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc
    27721 ggcgcgttcg ccaatatcaa tcgcccgttt tctggtccga cgcatgaaaa aacgctgccc
    27781 gttggcaaac atccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg
    27841 attatgcttg aggagctact ggcgctgggc gttactggtg cagagtacga cgcctggctg
    27901 attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg
    27961 aagatcccgg cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgaatctggt
    28021 tcgatcctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaaa
    28081 cggactgaaa cgttgaactg gctgttctgg ttacagggtg ccgcgccgtt cctcggcggt
    28141 ggttttggtc acttttacca ttacgcgccg gtgaaaattg agtacgccat caaccgcttt
    28201 accatggaag ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt
    28261 gttgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg
    28321 gtgctcggcg gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacatgtg
    28381 caacgctggg cgaaagaagt gggcgaacgt ccggcagtga aacgtgggcg tattgttaac
    28441 cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc atgacgccag tgattttgag
    28501 acaaatacag aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca
    28561 ggccggataa ggcgttcacg ccgcatccgg caatcgtgca caatgcctga tgcgacgctg
    28621 ccgcgtctta tcaggcctac aaaagctaat catcacgcgc tggtaatgtc gtaatccatt
    28681 tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat
    28741 ccgacgtgta ccagcaccca ctggcccagc agatcggcag ggttaccttc gcaaatcagg
    28801 gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct
    28861 tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttatttc
    28921 gattgccgga caaacgcgaa aaagaacctt tagcgggtgt tccctctcac cctaaccctc
    28981 tccccggagg ggcgagggga ccgtccgtgc gctttttagg cgttgtcctc agtcttattc
    29041 aacttcaata cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg
    29101 acagagcgga cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca
    29161 ccaggcctgg gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg
    29221 gcagacaatt tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat
    29281 ttccagccac acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat
    29341 ttcaacggcg ctctggcaaa gcgacaactc atgcattttc gcccctcctg cggccaaaca
    29401 gcagggcgcg acgccctgcg tgtggtacat ccggattggt gactggcaac gaaaggatca
    29461 ttcgtgcgca gtcatccgtc aggcgttggc cttcttcaat cgacatgctg tgcgaaagcg
    29521 gcgacatcag cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg
    29581 tcatggtgcc atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga
    29641 gttgatccgg gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg
    29701 tccactgacc ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa
    29761 aagagagatc gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct
    29821 tcggggaggt ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct
    29881 tccacgcctg attcgcgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct
    29941 tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg
    30001 gtcagctttt tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca
    30061 agctgatgcg gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc
    30121 atcatcgttc ccggagtgtt ctttttcgac acaatggcat ccgcaataat cagatgatcg
    30181 cgatttgcca tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca
    30241 taatccggca gaatgtatcg ttgctctaac gcttcgacaa tccgcacgcc gatggcttca
    30301 tcggtcagca aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg
    30361 aaaccacttc gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga
    30421 aggagtgaat ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca
    30481 ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg
    30541 ttgatggaac aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag
    30601 agagcatacc gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa
    30661 tgttcggctt cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt
    30721 attggttttg caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc
    30781 ccagcgtgga gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg
    30841 caacgatttc attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca
    30901 tattagccag cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg
    30961 aatatttccc gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggcg
    31021 cctgtggcgc ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca
    31081 ggtattcatc ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt
    31141 agcctcccgg gaacaggaag ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca
    31201 ggtagttcac cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg gcgataaccg
    31261 cggtatcaac cttataaacc tgctcaacaa agtcgctcag tttgtcgatg aaagacttga
    31321 tgtacatcag gcgctcaagg ttcaacacgc ccaacccgtc gaggttgatt gggttcgcga
    31381 caccacccac agccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc
    31441 ggttagcgtc acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca
    31501 cttccggcgg cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact
    31561 gaccgctggc aaccagatct ttgatcttgt tctgaacttt agtgaactct tccggactgt
    31621 tcagatgcca ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca
    31681 gtgcagaagt gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt
    31741 catgcgtggt gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt
    31801 cgatattcag cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata
    31861 cgccacagat acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct
    31921 cttccatgcc gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga
    31981 tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct
    32041 ggctcatgct ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat
    32101 cggcagtaga cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga
    32161 aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac
    32221 caacgaatag gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa
    32281 cagcatacgg gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat
    32341 cagcaggact tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag
    32401 cttgtcgcga tagataagct cgccaaagcg cagcccgatg aaaatcgcca gcaacacact
    32461 gatggtgttg gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc
    32521 cgcctgcacc agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag
    32581 cagcgagaac agcggcaaca tttcatagct ctgccagagc ggatgcacct tgtagcccgc
    32641 cgagatcatc agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc
    32701 gatgatgaag aacatcacct tgttcagacg ttgtagcgac accttccagc ccagacgttc
    32761 aaacagtgcc ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc
    32821 cgtctcgaac agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt
    32881 ccagtagcga cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact
    32941 cgccaacagc gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc
    33001 ccacgccagc gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat
    33061 ccacacgccc cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag
    33121 acgcttcaca atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt
    33181 actgatgatt ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt
    33241 gatggtcgtt tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg
    33301 gtagcatcat gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa
    33361 gatcgtccag tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct
    33421 gagtaccgcc gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca
    33481 ggtaagtgtc gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca
    33541 gcgccagacg ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc
    33601 ccgccgggca cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt
    33661 tctggttgca cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg
    33721 gcacgttgta cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt
    33781 aatggacaat gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac
    33841 agttcggatc gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct
    33901 ggtctttgtt gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg
    33961 acagtttgtc gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga
    34021 tatcctgaca cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca
    34081 tccccagcga tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca
    34141 gcgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg
    34201 ttattctccc cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc
    34261 catcacgctg acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc
    34321 agagacgttg ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt
    34381 ttcgacgttg gcaagctgat ggatgccttt atggaagccg ataccttttt cgttacagcc
    34441 atagcacggg tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt
    34501 tgagcagttg ccgtaagttt ctggcccttt acagccgagg tggtacaagc accagccttc
    34561 gcggtggcct tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg
    34621 ttcgcagtgt tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag
    34681 tttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt
    34741 cggcgggcag cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac
    34801 tgcgccagtt gggttaactc cagctgcggc aacaccgccc cacgcggagc aggaaccaat
    34861 agcgataatg gctgctgagc cttccgccgc tttgcggatg tgatccacaa tcggctcacc
    34921 ggcaaccatg caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac
    34981 atactgccct ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa
    35041 ggcggcggaa agcacttcgt gatattccag agagatagtc tccagcacga ggttttctac
    35101 cgttggatgc gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat
    35161 ccagataact ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt
    35221 gctacttaac cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt
    35281 aatgccgtga gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgag
    35341 aacctggcat atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccattcac
    35401 cacacattta ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga
    35461 agaagcatta gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg
    35521 cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc
    35581 attaccatct ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta
    35641 agctggcgat ctccggtctt ttcgtacatc ctgtgtgagt agcctaatac aaaaccgcgt
    35701 ttataatcgg cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat
    35761 cccgccatta agcctttacc aaaatggtta ttcatcgttc acctcattca ttatatatcg
    35821 ccatatataa cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatactt
    35881 gtttaagacg taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt
    35941 cagtgtcctt tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt
    36001 atggaatccg tgattaaccc ccggatagat ataagcctca taaaccttat tattgtcttt
    36061 caacgccgcc tcgtaaggag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg
    36121 gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa
    36181 cggcaccgcg caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc
    36241 atagcaaaat ccggtaatac ccactttacc tgtcgcttgc ggatagcgtt gcataaactc
    36301 aattgcggca aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg
    36361 acctttatca tcatttcccg gataacctcc aacggaactt aatccgtcag gtgccagggc
    36421 gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg
    36481 attctcatgc accaccccca cggctggcgt tttgccgctc atttttgcgg gtttcaccag
    36541 ataaccccgt acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat
    36601 ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa
    36661 caacgctaat gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg
    36721 tttcgaaatt ttcccgtgaa cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc
    36781 tttggcggtt aaacgcggca tcgttgcatt cctcaactgg tgttttttta agcaaagcat
    36841 aagcgcgtgt ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct
    36901 ggcgaatcgc taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg
    36961 gtctggttag cgaatcccga acgttacggg cagatgcagt accgctattg cggaaaaagt
    37021 ggtttacgcc tgcccgcatt atcgctcggt ttatggcaca atttcggtca cgttaacgcg
    37081 ctggaatcac agcgtgcaat cctgcgtaaa gcgtttgatt taggcattac gcactttgat
    37141 ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg
    37201 cgcgaggatt ttgctgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat
    37261 atgtggcccg gcccgtacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac
    37321 caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat
    37381 gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag
    37441 gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg
    37501 ctgcacgagt ggaaaattcc gcttttaatt catcaacctt cgtacaattt actgaaccgc
    37561 tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgtattgcc
    37621 tttactcctc tggctcaggg attgctgacc ggaaaatatc tcaacggcat tccgcaagat
    37681 tcacggatgc atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa
    37741 gccaacctca acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg
    37801 gcgcaaatgg cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt
    37861 gccagccgcg cggagcaact agaggagaac gtgcaggcgc tgaataatct gacatttagc
    37921 accgaggagc tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag
    37981 gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga accgcgcccg aatcagatca
    38041 gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt
    38101 cagatggata atgacgtacc acatcagttt gttatccggg acatttttcg catccataaa
    38161 gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag
    38221 cgacgttgcg tccattttcc ccagccactt cagcttctct ttgttctcgg agatatccag
    38281 ctgtgagacg aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag
    38341 cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat
    38401 attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag
    38461 cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag
    38521 aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag
    38581 taaacagcac gcaacagcaa atcaacatat acgttaaacc tttgtcgcat aattctgagg
    38641 tttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt
    38701 tgaacctgaa gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac
    38761 tggtgaatat cccaaacaga aacaaccgac gccaagcatc caggcgaaga tgacaccgat
    38821 accggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact
    38881 ggtgacaggg ggcgattccg gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg
    38941 ggctgacgtg gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa
    39001 gatcattgaa gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa
    39061 attcgcccgt tcgctggttc acgaagcgca caaggcgtta ggcgggctgg atattatggc
    39121 gctggtcgcc gggaaacagg ttgccattcc ggatattgca gacctcacca gcgaacagtt
    39181 tcaaaagacc tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc
    39241 cctgctaccg aaaggtgcaa gtgtcatcac cacttcgtca atccaggcat accagccaag
    39301 tccgcattta ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct
    39361 ggcaaaacag gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gccctatctg
    39421 gacggcactg caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa
    39481 aacgccgatg aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc
    39541 aagtcaggag tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt
    39601 aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac
    39661 ctgatgtggc gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat
    39721 agattttgta ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg
    39781 tcgtgaacgc cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt
    39841 gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct
    39901 tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc
    39961 aacgtctcgt aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct
    40021 tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata
    40081 aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc
    40141 gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc
    40201 ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc
    40261 atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg
    40321 cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca
    40381 cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca
    40441 atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt
    40501 gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc
    40561 gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg
    40621 aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc
    40681 actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg
    40741 tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag
    40801 cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc
    40861 agcagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg
    40921 aagaagattg cccaggtgac tacagaggcc aaaataagcc caatcatcac gcacttaacg
    40981 acaatatcgg cgtactgata cataccccag acggaaaggt ccgtctgcat taaattatta
    41041 cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac
    41101 gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc
    41161 cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat
    41221 gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa
    41281 aaacaggaat cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg
    41341 cagcaaaaaa tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt
    41401 atttgagagt atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt
    41461 ctatggacgg cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga
    41521 aggtggcgca ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct
    41581 tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca
    41641 ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct
    41701 gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc
    41761 gccgggttcc atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag
    41821 tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa
    41881 ggcgctggat tttggcatcg atgtttctat tcaggccgcc accaaatatc tggttgggca
    41941 ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga
    42001 aaatgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg
    42061 cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga
    42121 atggctggca gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa
    42181 aggacacgaa ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct
    42241 taagaaaaaa ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag
    42301 catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat
    42361 cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat
    42421 tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg cacggattgt
    42481 ataacattgc cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt
    42541 tgcgtccggg atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta
    42601 aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc
    42661 aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt
    42721 ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct
    42781 tgttgatact ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg
    42841 cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag gggagctggt
    42901 tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc
    42961 gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg
    43021 catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct
    43081 tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct
    43141 atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga
    43201 tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta
    43261 aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt
    43321 atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag
    43381 taacgtttaa actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct
    43441 gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc
    43501 ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat
    43561 acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc
    43621 tcaattcgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc
    43681 agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt
    43741 ttgccgagaa tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg
    43801 cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg
    43861 aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg
    43921 agacgcaacc ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac
    43981 ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa
    44041 ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt
    44101 gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga caaactattt
    44161 tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc ttcacgtttc
    44221 atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt
    44281 tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg
    44341 cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag
    44401 caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt
    44461 aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc
    44521 tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa
    44581 ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg
    44641 aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt
    44701 tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat
    44761 ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt
    44821 gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa
    44881 accacaggcg acaagcaggc gttccattct gcacatgttc agccggtatt tgccgtgctc
    44941 gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc
    45001 tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt
    45061 ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca
    45121 gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg
    45181 attggcactg gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg
    45241 atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa
    45301 aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact
    45361 gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag
    45421 caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct
    45481 ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg
    45541 ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt
    45601 cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt
    45661 gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca
    45721 atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg
    45781 ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga
    45841 acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca
    45901 tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc
    45961 tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta
    46021 tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca
    46081 aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg
    46141 gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac
    46201 acgcttggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag
    46261 ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc
    46321 cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg
    46381 tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg
    46441 aactcggcaa aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt
    46501 tcggcggcta acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag
    46561 aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa
    46621 atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg
    46681 ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc
    46741 ctggcaagtt ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg
    46801 cagatttatc agaactataa atcagatgcc agtacgcctt atttcggtga aaccggagag
    46861 cgggcgataa cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacacta
    46921 ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat
    46981 ctggtgttag aaagcgtagc gataacccaa actgacgtcg atcatgccga tgccgttgtg
    47041 cgctttcgta tctttaaaga tgataaagaa aagaccacac agacactgaa aatggtggcg
    47101 gaaaatggtc gttgggtcat tgatgatatt gtcagcaatc atggcagcgt cttacaagca
    47161 gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcagaa agaacagcca
    47221 gaatcctttg ttgctgaact ctttgaacat attgctgatt atagctggcc gtggacgtgg
    47281 gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac cttcaagacg
    47341 gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc
    47401 tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc
    47461 gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa tgaagagcaa
    47521 gagctggttt tacagcggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac
    47581 agcggcagcc tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct
    47641 gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt
    47701 tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa
    47761 aggtcaatca actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc
    47821 aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca
    47881 atacgcgtct tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag
    47941 aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta acgtccaacc
    48001 gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt agacgacgct
    48061 ttttttgccg tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt agtcaacgcc
    48121 ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt tggcgcaggc
    48181 actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg
    48241 cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc
    48301 tgtttgtcgc ccttcggtac gaagacgcct tcgctcttat aaccaatctt cgccagcggt
    48361 tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta gaagttctgt
    48421 acctggtcga gacggaaacg atgctttttc agccacagcg ccaaattcac catatcttca
    48481 tcacgcgtac cggggtgcgc agagatgaaa tacgggatca gatactgctc tttacctgcc
    48541 tgtttcgagt aggtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc
    48601 atcatcttcg acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg
    48661 acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat gtcataacgt
    48721 acgcctgagg cgataaggat ttttttaatg cctttcagat cacgcgcacg gcgatagagg
    48781 ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata aacgcacgac
    48841 aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata catgttggca
    48901 gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct
    48961 tcgatctcat taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg
    49021 gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac cgaaaaacgg
    49081 atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac gcgcttgtac
    49141 ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg ggatagcagg cgggttgatc
    49201 cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg
    49261 tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa
    49321 gaaggcagca atacgtaggt tttttcccac ggtttctggc gcggtggctg cacgattaca
    49381 gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg caaatcttcg
    49441 ccatacggat gcgggattgg gtcgattttc cctagggtat caagacgggt ggaatccaca
    49501 ccgctccagc caggcagcgc ctcttttacg ataattgcgg tattacgcac atcgcggatt
    49561 tcactaatcg gctcgcccat cgccagacga tgcgctactt ccaccagcgg acgttcaccg
    49621 ttaccaaaca tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg cacggtatcg
    49681 gaccagtaat cataatgcgc ggtacggcgc aggctagcct caataccgcc gaggatcacc
    49741 ggcacatctt tccacgcctc tttacaacgc tgggtataaa ccagcgtggc gcgatccggt
    49801 cgcttacccg cgacattatc tggcgtgtag gcatcatcat gacgtaaacg gcgatcggcg
    49861 gtatagcggt tgatcatcga atccatgttg ccagcagtaa caccaaaaaa cagattcggt
    49921 ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat gatcccgacg
    49981 cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg
    50041 acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc
    50101 tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc
    50161 caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa
    50221 atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaag
    50281 gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgaccaaca
    50341 gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga
    50401 agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa
    50461 cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct
    50521 ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt
    50581 cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc tgtccattaa
    50641 tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca gccatgattt
    50701 cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc
    50761 gcagcgttag caccaaggta gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac
    50821 gatcaacaat gctcgccgct tcaccacagg tgatcgacac ttcatcgccg ttgctcatat
    50881 ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag
    50941 caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta
    51001 gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga
    51061 cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc
    51121 ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga
    51181 ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg acttcatctt
    51241 ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat
    51301 tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt gcccagtcag
    51361 cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg
    51421 ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc
    51481 ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc
    51541 gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac
    51601 ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct
    51661 tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac
    51721 tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc
    51781 cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc
    51841 tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag ccagctcacg
    51901 aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag
    51961 accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat
    52021 aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc
    52081 gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa
    52141 atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa
    52201 taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt
    52261 acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt
    52321 ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca
    52381 ctcaactttc aggccgaaca gtggcgcaag acggccaaac atatgcccaa aggtggccac
    52441 atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat
    52501 gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct
    52561 gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc
    52621 tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta
    52681 tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct
    52741 tgctagcggg aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg
    52801 gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta
    52861 ccgcggcgtc cacgttcgcc agtgactttc tgtagctctt ccggacgcag tttaattttg
    52921 cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga
    52981 ccatcctcgc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc
    53041 ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt
    53101 gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc
    53161 ggtaaggtga tcaaagcctt acccgcacgg ttacgcgcta ccagatcgtt aaaggtgcag
    53221 acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc
    53281 atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg ttcgccctga
    53341 ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca
    53401 aacactaccg gttggttact cttacctttc accgccgctt tgaagctatc acccgcttta
    53461 taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc
    53521 gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct
    53581 tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc
    53641 agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg
    53701 tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca
    53761 agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc
    53821 gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg
    53881 ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga
    53941 cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt
    54001 tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga
    54061 tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg
    54121 cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc
    54181 agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg
    54241 cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc
    54301 cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg
    54361 cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc
    54421 tgatcgagcg tagttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc
    54481 aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc
    54541 aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc gtcgaagttt
    54601 ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac
    54661 aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag
    54721 ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc
    54781 gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta
    54841 cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca
    54901 atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc
    54961 acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct
    55021 gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata
    55081 ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc
    55141 cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc
    55201 tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca
    55261 tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa
    55321 gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg tctccaccag
    55381 tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tatccccgag acggcttacg
    55441 gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa attataagcc
    55501 caacaacaat acgcctgtga cattgttcgc tcaaacttat cgcgagtgat ttggctcaca
    55561 ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct
    55621 ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct
    55681 cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca
    55741 gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac
    55801 cataacgatt atcgggaatc agttcgctaa cgctgccgca gatatcaaag cgaaacgctt
    55861 ccggtgcggt ggtggcaggg gcgtcccagg cgacgccaaa agtctggctt tggttaacag
    55921 gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact
    55981 cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta
    56041 catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc
    56101 gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa
    56161 ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt caccgtcaca
    56221 ccgttccagc gtttgtgatc gatatcaaca ttgatagtac cgctggcatc tttaaacacg
    56281 tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca
    56341 cgcagggatt ttgcgctttc tacagtcgtt acgctgccgt tcggcccctg aaatcctccg
    56401 gcctgacttt gcgttaccga tgggccagaa aaaccgccct gctctgctgc catcaccggt
    56461 gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc
    56521 tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa
    56581 aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt
    56641 tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggtgac
    56701 ggcatcaaaa ctggccttag taaaatgggt tttagtgtcg actggtttac acaaggtcgt
    56761 cagggaaaag aggcgcttta tagcgcacct tatgatgcgg tgatcctgga tttaacctta
    56821 ccaggcatgg atggtcgcga tattttgcgc gaatggcgag agaaaggtca gcgtgagccg
    56881 gtactgaacc tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct gcgtctggga
    56941 gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag gctggaggct
    57001 ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc
    57061 gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa
    57121 tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt
    57181 gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg
    57241 catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac
    57301 acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa
    57361 tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa
    57421 caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aaacggttaa
    57481 gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat
    57541 taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca
    57601 gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat caacgggaag
    57661 gttttgctga cgggcaactg gtcggtgaag acgatccttg gcgttttgtc tggatgacct
    57721 cacctgatgg caaatatcgc atcgttgttg gccaggaatg ggaataccgt gaagacatgg
    57781 cgctggcgat tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa
    57841 tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac
    57901 tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta cccagcgaag
    57961 tgcggccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc
    58021 gagaacgacg ctttacctcc gatgcagctc acgaactacg tagcccgtta acagcgctga
    58081 aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg aaaaaagcac
    58141 tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcacgc
    58201 tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc
    58261 tcctacaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc
    58321 gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt
    58381 tggtgcgaaa tttgctggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg
    58441 gcgaacgctt ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcga
    58501 ttgtccagcg aatcgccaag ctgcatgaca tgaatgttga gtttgggaat gcggaacaag
    58561 gtggatttga ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac
    58621 tctcatagat tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa
    58681 accagacaaa taacgagcga gaatacatat agccaaaact cgaacatttc ccgcataaag
    58741 agttttctta agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct
    58801 tgcaatatac gatcttcgcc tgaaacggaa ggtatactgc aaatattgcc tcccgactca
    58861 agcatccagt catacttatt gaaagcaaaa aaagaatcta ttaaccaata agtaagcatt
    58921 atatatagca atgcttcaaa tattgtttta attttctgct ttaacatata tcagtacgct
    58981 cataaaaatc catttataat tatcaacatg acacgttgat attttcatct tttacttatt
    59041 caaagccata ttttcaatcc ccaaaaaaac cgaaccaaac gtgtaaatta ttccggtgcg
    59101 agtaagaatt atgaggaatg gctatcagta ttgtcatttt cagaaaatat ttatcctgca
    59161 tcggtgagtc agagctagat cagacttttg ctaaattcgc aaaagacttt gcacattttg
    59221 ctaatttcac agtaccgctc tgtgacgtac tatagtcgac aaacgtctca ccttgagatt
    59281 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    59341 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    59401 tccgcatcgt tcgcgccgac agcgactaca atgtcaaagc ggaagtacaa aactttctct
    59461 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    59521 aaaaatatat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc
    59581 gtacccgcaa agatccgtcg aaaaaatacg gttccggcag cctggtacag gacaaaaaat
    59641 atatgctttc tctaacctgg aacgcaccaa tggaagcctt caccgaaaaa gatcagttct
    59701 tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg
    59761 gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    59821 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    59881 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    59941 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    60001 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    60061 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcac cttgaagcgc
    60121 atctgcaaac cccgcacatg aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga
    60181 tgaatatccg tattctgcag ccagggattt aatcctgcct tgtttgcccg gccatcctga
    60241 ccgggcaatg ttctttcctt taaacctcaa tctccgccat gtcgcctttc tcttgcaacc
    60301 agttgcggcg atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac
    60361 gctgatcgtc ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg
    60421 tggtttcacg caattgcatc gggttcattt cccccagacc tttaaaacgc tggacgttcg
    60481 gcttgccttt cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg
    60541 cgtaataaac ctctttcccg agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt
    60601 gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa
    60661 tgtgcagacc atcagagtcc gcatccgcga ggatacagat tttgccataa cgaagctggc
    60721 tcagatcgtc gctgtcagga tcgataccga tcgctaccga aatatcatgc acttcctgcg
    60781 aggccagcac ttcgtcggaa gaaacttccc aggtgttaag gatcttacct ttcagcggca
    60841 tgatcgcctg atattcacga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt
    60901 ccacgaggaa cagttcggta cggttaaggc cctgcgcggt acaatcagcc agtttgccag
    60961 gcagcgccgg gccgctggtc agctttttac gcaccacttt tttagccgca cgcatacgac
    61021 gctgggcgct ggaaatcgcc atctctgcca gcaactccgc cgcctgaacg ttctggttca
    61081 gccacaggat gaaggcatct ttcaccacgc ccgaaacgaa tgccgcacac tgacgcgaag
    61141 agagacgctc tttggtctgc ccggcaaact gcggatcctg cattttaacc gacagaacat
    61201 aggcgcagcg atcccagata tcttccgccg acagcttcac gccgcgcggc agaatgttgc
    61261 ggtattcaca gaactcacgc atcgcgtcca acaggccctg acgcagacca ttaacatggg
    61321 taccgccctg catcgttggg ataaggttga cgtagctttc ggtcagcagt tcaccgcctt
    61381 ccggcagcca cagtagcgcc cagtccacag cttcagtatc gccagcgaaa ttaccgataa
    61441 acggtttttc tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac
    61501 cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc atctttaaag gtaatctcaa
    61561 cgccggggca cagtactgct ttggccttca gcacatgcgt caggcgtgaa acagaaaaac
    61621 gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt
    61681 taccgcaagt gccgacaacc tgtaaatcct gcaccttttc gccattttca aaggcgatgt
    61741 tataaacctg accatcgcgg cgcacgttaa cttctacgcg cttcgacaag gcgttaacta
    61801 ccgaaatccc cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac
    61861 cgcctgcatg cagacggcaa agaatcagtt caaccgccgg tactccttct tccgggtgaa
    61921 tatccaccgg catcccgcgc ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga
    61981 ttacatccac gcgttttgcg tgacccgcca gtgcttcatc gacactgtta tcaataactt
    62041 cttgccccaa atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg
    62101 gctcaagccc ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag
    62161 ttagcaattc gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg
    62221 aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag
    62281 gaagcatagt acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc
    62341 agccagatca aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata
    62401 tggcgtgact ctagcacata ttgctgcccg gtgtaggggt tctcgttctg accgagatag
    62461 tccgtcagca attcaaacgg gcgtaccgcc gggtttacca ccactgcggg cagcataaaa
    62521 cattgcgaca accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa
    62581 tcgccgccat gttccaggac aatggattcc agcagttctg ccgcgtcgga aggatacggc
    62641 ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac
    62701 aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac
    62761 atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct
    62821 cggtggtcag cgtgccatca gcatgtaact cgagagtacg ccagccgggc gcgatagtat
    62881 ccagcgtaaa gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca
    62941 ggcggcgacc gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt
    63001 tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc agactgtgtt
    63061 gatcgagcca actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc
    63121 gttctggcgc atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac
    63181 cgtgcggcac gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa
    63241 acacccgctt cgccggggag atacccgcat cctgtaacgc gctgtacatc gcgggctgga
    63301 aatcgtggtt gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga
    63361 aatgctgata ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt
    63421 cgaattcgtg ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt
    63481 ttacccctaa cagggcttcg tgcttttgtg caaacaggtg agtatcggta atttgtaaaa
    63541 tccttcctct ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt
    63601 aggtttcacg acgctagtaa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca
    63661 accagtccgc taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc aactttttat
    63721 taggataatc ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca
    63781 ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg
    63841 gcgcagtctg ttctattgtc accagggtag tgtatcgggt cgattccaca atcgtcagcc
    63901 gatattgtgc gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta
    63961 acaaacggcg caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag
    64021 gtgtgtaacg cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc
    64081 attgcaaagc gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg
    64141 cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa
    64201 taccgcttgc ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc
    64261 cccccgggct tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct
    64321 cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc accctcttca atcatcccgg
    64381 caaccatctc cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct
    64441 caatcagcac aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc
    64501 gctcaaaaat ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa
    64561 atctataaag atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta
    64621 catcgttttt gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctag
    64681 cactaatagt gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcata
    64741 tgttaaattg aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg
    64801 cttctgctag aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca
    64861 aggaatgcaa atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag
    64921 ttcgttgagc caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc
    64981 ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg
    65041 cagtccatta ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg
    65101 cgacgcgaac ggcatcaact cgaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat
    65161 ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga
    65221 cgtcacatat cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa
    65281 cgtgttgaat gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg
    65341 tcaattagat caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca
    65401 gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct
    65461 tgataacgcg gtagagcagc tgcgccagat caccggtaac tactatccgg aactggcggc
    65521 gctgaatgtc gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga
    65581 agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggagcgcga
    65641 gcaaattcgc caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg
    65701 gatttctgac acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga
    65761 cgatagcaat atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg
    65821 cggaatggtt aactcgcagg tgaaacaggc acagtacaac tttgttggtg ccagtgagca
    65881 actggaaagc gcacatcgta gcgtcgtgca aaccgtacgt tcctccttca acaacattaa
    65941 tgcttctatc agtagtatta acgcctacaa acaagccgta gtttccgcgc aaagctcatt
    66001 agacgcgatg gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc
    66061 gaccaccacg ctgtacaacg ccaagcaaga gctggcgaat gcgcgttata actacctgat
    66121 taatcagctg aatattaagt cagccctggg tacgttgaac gagcaggatt tgctggcact
    66181 gaacaatgcg ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc
    66241 ggaacagaat gctattgctg atggttatgc gcctgatagc ccggcacccg tcgttcagca
    66301 aacatccgca cgcactacca ccagtaacgg tcataaccct ttccgtaact gatgacgacg
    66361 acggggcttc ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat
    66421 tcttccccct tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca
    66481 ctggtcccgg aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa
    66541 aaactggagc gcacgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct
    66601 ggctggctgt gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc
    66661 agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga
    66721 agccgaacgt actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga
    66781 aggtcagtgt cagcaagcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc
    66841 tcagcaatcc agcgggagtt tctggatgcc gctgatggcc ggttacatga tggggcgtct
    66901 gatgggcggc ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccggccag
    66961 tccggcttac ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag cccagccagg
    67021 ccgcaccatg accgtaccga agacggcaat ggcaccaaaa ccggcgacca ccactaccgt
    67081 tacccgtggc ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac
    67141 cggcacctct tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag
    67201 cgcccggact ggcgtgagaa agcccacgaa tacggtttca attttcacac catgtacggc
    67261 gagccgtact ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt tgaaaagctg
    67321 gaagaagtca ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc
    67381 agcgatgagc tgatgaccaa attccgcata ccaaaacaca cctggagttt tgtgcgtcaa
    67441 tcatggctga ctcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact
    67501 ggcgaaccta aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg
    67561 ttctttcagt ggatctggct ggaagatcag cttaacgccg gtaacttgcc ggagggcagc
    67621 gaccagttta acagtctgca agaaaaactg atcgatcgct tcgttgagct gcgtgaacag
    67681 tatggcttcc agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc
    67741 attcagtatt tgcaggactg cgcaaccgaa gctgaaatcg ccaccgagtt cctctacatc
    67801 gacgagatag gtttaggcga aaaaggccag ttcacggatt tacaggatca ggtgatttct
    67861 aacctgttca agctttatcc gtgggaattt atgctgcgcg aaatgttctc caccaagctg
    67921 gaagatgctg gcgtacgctg gctggaaccg gcgtggaaga gcattatctc caacaaggca
    67981 cttctaccgc tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt
    68041 gcggaagatg atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa
    68101 ggcgcaaacg tgtcgatcat tgagaacggc aaaaccattg aagcagcgga aggtccgtat
    68161 ggcgaagaag ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat
    68221 atgctgattg gtagctggct ggtgaacgat caacctgccg gaattggcat tcgtgaagac
    68281 cgtgcgttga tcacccagga tatgtctcgg ttttatccac atatttttgt tgaataagcc
    68341 acgataccgg atggcactcg ccatccggta attgttagcc tatctgcacc gacagcatac
    68401 ttaaactgcc catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccaca
    68461 cacctaacac atacaacaac ggcagatagt gctctggcgt tgggttcaat aacgcgccac
    68521 cttcatggtc gagataattc accagaggat gctgctctac cggcccttgc cacgtcagat
    68581 tcgctttcac atactcatta aacgacgtcg cccacggata cggtgaacta tcaccgtgcc
    68641 acttcactgt gcgcaggtta tgcaccacgt taccgctggc gaccaacatt attccttcat
    68701 cacgcagcgc cgcgagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt
    68761 cgatactcaa ctgcaccata gggatatcgg cgtcaggata catcttaatc agcacgcccc
    68821 acgagccgtg gtcaaagccc caggcttctt tatccagcgt caccgggatc ggcgctaaca
    68881 gctcaaccag acgctgtgcc agcgcaggcg aacccggagc aggataatgc gtatcgtaca
    68941 gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc
    69001 ctgttccacg ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg
    69061 tcatccccaa cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac
    69121 taccgtgacc taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta
    69181 aaggtgtcat tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac
    69241 aatgatgatg atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt
    69301 tgtcgatgtc agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg
    69361 cgtttctcgg cgttctcgga caaaaaccct cgaaccgctt actggcgttt tcgctaggtt
    69421 ttgcggcggg gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag
    69481 ctgaaggaat gtcgccggtg ttgggttatg gaatgtttat cttcggtctg cttggctact
    69541 ttggcctgga ccgcatgttg ccgcatgcgc atccgcagga tttaatgcaa aaatcggtgc
    69601 agctgttgcc aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc
    69661 ataacttccc ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggat
    69721 ttggcatcgc actggccgtc gcgttgcaca atatccctga aggtctggca gtggcaggcc
    69781 cggtttatgc ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg atttctggac
    69841 tagcagaaat tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg
    69901 tggtcatggc ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg
    69961 aattaatgcc gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat
    70021 gtggaatgtc agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa
    70081 aacaacatca gaagttgtcc agcacatgga caacttctgc cagcttcacg aaagccttat
    70141 tctacctttt cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc
    70201 agtgcgtctc tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc
    70261 accacaacca gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta
    70321 atatttaaca atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt
    70381 catatggtcg cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc
    70441 tgatattaat gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg
    70501 caaaataaaa accgaagcca gctacaactt cggtttcttg attaaggcag taattaagca
    70561 gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccagg tcttcaatag
    70621 tcacgagcgc catattgtgt ttattggcaa actcaatgca ctctggtgca cgcgccatcg
    70681 tgccatcgtc attagtcagc tcacacagca caccagccgg tttaaagcct gccagcgtca
    70741 tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc
    70801 gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca tcggcaatcg
    70861 ctgcgcgaac ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg
    70921 cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct accatcattg
    70981 gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata ccgctaccgt
    71041 ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag atcatatcgc
    71101 cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca cgcagcgcag
    71161 ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca
    71221 tggtaaaaaa acctcactaa aattatggtt accagaatca gggcagtctt aggagtggcg
    71281 gcatatagcc aaaataacgt gagcgggtcc atgcccgaca gaatcgttac tctctcccat
    71341 ccggactcta accgtcggcc ccggaattac accggatctg ctgtcctttg agttcgcacc
    71401 caaagcgctc gcgggctttc aactgagttg atttaccgcc ggtggggaat ttcgccccgc
    71461 cctgagaata agcggattca ctataacgct aatgattagc ggcagcaacg catagcttca
    71521 cataattctg gtttatgact tacccttatc gcactacaat ggtactcaac acctatcagt
    71581 acagggaaac tacaatgatt gacccgaaaa aaattgagca aatcgctcgc caggttcacg
    71641 aatcaatgcc taaaggaatc agggagttcg gggaagatgt ggagaaaaaa atccgccaaa
    71701 ccctacaagc tcagctgacg cgcctcgacc ttgtaagccg tgaagagttc gacgtccaaa
    71761 cgcaagtcct gttacggact cgtgagaaac tggcgctgct tgaacaaaga atcagcgaac
    71821 tggaaaatcg cagtactgag atcaaaaagc agccagatcc agagactctc cccccaacgc
    71881 tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta atcctctaat ttacagtgtt
    71941 ttctattaca ttatcgctta atcccattca gagtatcatc acaaaaatga atgattgcaa
    72001 ttgaaaatat aattgttatg tattctttaa gttttac
//
LOCUS       DAJTNP010000006        70449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTNP010000006 DAJTNP010000000
VERSION     DAJTNP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..712
                     /locus_tag="OO160_000458"
     CDS             <1..712
                     /locus_tag="OO160_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5522704.1"
                     /translation="LLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGN
                     DSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAV
                     VPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSD
                     RNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFS
                     TPDSEPTTSVLQGTAQCH"
     gene            723..1814
                     /locus_tag="OO160_000459"
     CDS             723..1814
                     /locus_tag="OO160_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5522705.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1857..2717)
                     /gene="rsmI"
                     /locus_tag="OO160_000460"
     CDS             complement(1857..2717)
                     /gene="rsmI"
                     /locus_tag="OO160_000460"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5522706.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2782..4818
                     /gene="lpoA"
                     /locus_tag="OO160_000461"
     CDS             2782..4818
                     /gene="lpoA"
                     /locus_tag="OO160_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5522707.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4776..5171
                     /locus_tag="OO160_000462"
     CDS             4776..5171
                     /locus_tag="OO160_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5522708.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5191..5781
                     /gene="diaA"
                     /locus_tag="OO160_000463"
     CDS             5191..5781
                     /gene="diaA"
                     /locus_tag="OO160_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5522709.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5791..6366
                     /gene="yraP"
                     /locus_tag="OO160_000464"
     CDS             5791..6366
                     /gene="yraP"
                     /locus_tag="OO160_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5522710.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6480..7520)
                     /locus_tag="OO160_000465"
     CDS             complement(6480..7520)
                     /locus_tag="OO160_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5522711.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVSGLALLF"
     gene            complement(7593..8228)
                     /locus_tag="OO160_000466"
     CDS             complement(7593..8228)
                     /locus_tag="OO160_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5522712.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8356..8874
                     /gene="yhbO"
                     /locus_tag="OO160_000467"
     CDS             8356..8874
                     /gene="yhbO"
                     /locus_tag="OO160_000467"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5522713.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8854..9297)
                     /locus_tag="OO160_000468"
     CDS             complement(8854..9297)
                     /locus_tag="OO160_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5522714.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9348..9650
                     /gene="yhbQ"
                     /locus_tag="OO160_000469"
     CDS             9348..9650
                     /gene="yhbQ"
                     /locus_tag="OO160_000469"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5522715.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9637..10140)
                     /locus_tag="OO160_000470"
     CDS             complement(9637..10140)
                     /locus_tag="OO160_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5522716.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10134..10658)
                     /locus_tag="OO160_000471"
     CDS             complement(10134..10658)
                     /locus_tag="OO160_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5522717.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10867..11862
                     /locus_tag="OO160_000472"
     CDS             10867..11862
                     /locus_tag="OO160_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5522718.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11871..12749
                     /locus_tag="OO160_000473"
     CDS             11871..12749
                     /locus_tag="OO160_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5522719.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12830..13837
                     /locus_tag="OO160_000474"
     CDS             12830..13837
                     /locus_tag="OO160_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5522720.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13955..15199)
                     /gene="mtr"
                     /locus_tag="OO160_000475"
     CDS             complement(13955..15199)
                     /gene="mtr"
                     /locus_tag="OO160_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5522721.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15353..17242)
                     /gene="deaD"
                     /locus_tag="OO160_000476"
     CDS             complement(15353..17242)
                     /gene="deaD"
                     /locus_tag="OO160_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5522722.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17235..17315)
                     /gene="yrbN"
                     /locus_tag="OO160_000477"
     CDS             complement(17235..17315)
                     /gene="yrbN"
                     /locus_tag="OO160_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5522723.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17422..18306)
                     /gene="nlpI"
                     /locus_tag="OO160_000478"
     CDS             complement(17422..18306)
                     /gene="nlpI"
                     /locus_tag="OO160_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5522724.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18415..20550)
                     /gene="pnp"
                     /locus_tag="OO160_000479"
     CDS             complement(18415..20550)
                     /gene="pnp"
                     /locus_tag="OO160_000479"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5522725.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20797..21066)
                     /gene="rpsO"
                     /locus_tag="OO160_000480"
     CDS             complement(20797..21066)
                     /gene="rpsO"
                     /locus_tag="OO160_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5522726.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21215..22159)
                     /gene="truB"
                     /locus_tag="OO160_000481"
     CDS             complement(21215..22159)
                     /gene="truB"
                     /locus_tag="OO160_000481"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5522727.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22159..22560)
                     /gene="rbfA"
                     /locus_tag="OO160_000482"
     CDS             complement(22159..22560)
                     /gene="rbfA"
                     /locus_tag="OO160_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5522728.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22724..25396)
                     /gene="infB"
                     /locus_tag="OO160_000483"
     CDS             complement(22724..25396)
                     /gene="infB"
                     /locus_tag="OO160_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5522729.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25421..26908)
                     /gene="nusA"
                     /locus_tag="OO160_000484"
     CDS             complement(25421..26908)
                     /gene="nusA"
                     /locus_tag="OO160_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5522730.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26936..27388)
                     /gene="rimP"
                     /locus_tag="OO160_000485"
     CDS             complement(26936..27388)
                     /gene="rimP"
                     /locus_tag="OO160_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5522731.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27595..27671)
                     /locus_tag="OO160_000486"
     tRNA            complement(27595..27671)
                     /locus_tag="OO160_000486"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27635..27637),aa:Met,seq:cat)
     gene            28019..29362
                     /gene="argG"
                     /locus_tag="OO160_000487"
     CDS             28019..29362
                     /gene="argG"
                     /locus_tag="OO160_000487"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5522732.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29370..30977)
                     /locus_tag="OO160_000488"
                     /pseudo
     CDS             complement(29370..30977)
                     /locus_tag="OO160_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31455..31541)
                     /locus_tag="OO160_000489"
     tRNA            complement(31455..31541)
                     /locus_tag="OO160_000489"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31505..31507),aa:Leu,seq:gag)
     gene            complement(31556..31888)
                     /gene="secG"
                     /locus_tag="OO160_000490"
     CDS             complement(31556..31888)
                     /gene="secG"
                     /locus_tag="OO160_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5522733.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32116..33453)
                     /gene="glmM"
                     /locus_tag="OO160_000491"
     CDS             complement(32116..33453)
                     /gene="glmM"
                     /locus_tag="OO160_000491"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5522734.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33446..34294)
                     /gene="folP"
                     /locus_tag="OO160_000492"
     CDS             complement(33446..34294)
                     /gene="folP"
                     /locus_tag="OO160_000492"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5522735.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34384..36318)
                     /gene="ftsH"
                     /locus_tag="OO160_000493"
     CDS             complement(34384..36318)
                     /gene="ftsH"
                     /locus_tag="OO160_000493"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5522736.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36418..37047)
                     /gene="rlmE"
                     /locus_tag="OO160_000494"
     CDS             complement(36418..37047)
                     /gene="rlmE"
                     /locus_tag="OO160_000494"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5522737.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37173..37466
                     /gene="yhbY"
                     /locus_tag="OO160_000495"
     CDS             37173..37466
                     /gene="yhbY"
                     /locus_tag="OO160_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5522738.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37622..38098)
                     /gene="greA"
                     /locus_tag="OO160_000496"
     CDS             complement(37622..38098)
                     /gene="greA"
                     /locus_tag="OO160_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5522739.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38346..39779
                     /gene="dacB"
                     /locus_tag="OO160_000497"
     CDS             38346..39779
                     /gene="dacB"
                     /locus_tag="OO160_000497"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5522740.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39965..41137)
                     /gene="cgtA"
                     /locus_tag="OO160_000498"
     CDS             complement(39965..41137)
                     /gene="cgtA"
                     /locus_tag="OO160_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5522741.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41153..42118)
                     /locus_tag="OO160_000499"
     CDS             complement(41153..42118)
                     /locus_tag="OO160_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5522742.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42245..42502)
                     /gene="rpmA"
                     /locus_tag="OO160_000500"
     CDS             complement(42245..42502)
                     /gene="rpmA"
                     /locus_tag="OO160_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5522743.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42523..42834)
                     /gene="rplU"
                     /locus_tag="OO160_000501"
     CDS             complement(42523..42834)
                     /gene="rplU"
                     /locus_tag="OO160_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5522744.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43092..44063
                     /gene="ispB"
                     /locus_tag="OO160_000502"
     CDS             43092..44063
                     /gene="ispB"
                     /locus_tag="OO160_000502"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5522745.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44290..44568
                     /gene="sfsB"
                     /locus_tag="OO160_000503"
     CDS             44290..44568
                     /gene="sfsB"
                     /locus_tag="OO160_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5522746.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44616..45875)
                     /gene="murA"
                     /locus_tag="OO160_000504"
     CDS             complement(44616..45875)
                     /gene="murA"
                     /locus_tag="OO160_000504"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5522747.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45930..46184)
                     /gene="ibaG"
                     /locus_tag="OO160_000505"
     CDS             complement(45930..46184)
                     /gene="ibaG"
                     /locus_tag="OO160_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5522748.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46344..46637)
                     /gene="mlaB"
                     /locus_tag="OO160_000506"
     CDS             complement(46344..46637)
                     /gene="mlaB"
                     /locus_tag="OO160_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5522749.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46637..47272)
                     /gene="mlaC"
                     /locus_tag="OO160_000507"
     CDS             complement(46637..47272)
                     /gene="mlaC"
                     /locus_tag="OO160_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5522750.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47291..47842)
                     /gene="mlaD"
                     /locus_tag="OO160_000508"
     CDS             complement(47291..47842)
                     /gene="mlaD"
                     /locus_tag="OO160_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5522751.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47847..48629)
                     /gene="mlaE"
                     /locus_tag="OO160_000509"
     CDS             complement(47847..48629)
                     /gene="mlaE"
                     /locus_tag="OO160_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5522752.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48637..49446)
                     /gene="mlaF"
                     /locus_tag="OO160_000510"
     CDS             complement(48637..49446)
                     /gene="mlaF"
                     /locus_tag="OO160_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5522753.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49656..50621
                     /locus_tag="OO160_000511"
     CDS             49656..50621
                     /locus_tag="OO160_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5522754.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50635..51621
                     /gene="kdsD"
                     /locus_tag="OO160_000512"
     CDS             50635..51621
                     /gene="kdsD"
                     /locus_tag="OO160_000512"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5522755.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51642..52208
                     /gene="kdsC"
                     /locus_tag="OO160_000513"
     CDS             51642..52208
                     /gene="kdsC"
                     /locus_tag="OO160_000513"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5522756.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52205..52780
                     /gene="lptC"
                     /locus_tag="OO160_000514"
     CDS             52205..52780
                     /gene="lptC"
                     /locus_tag="OO160_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5522757.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52749..53306
                     /gene="lptA"
                     /locus_tag="OO160_000515"
     CDS             52749..53306
                     /gene="lptA"
                     /locus_tag="OO160_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5522758.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53313..54038
                     /gene="lptB"
                     /locus_tag="OO160_000516"
     CDS             53313..54038
                     /gene="lptB"
                     /locus_tag="OO160_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5522759.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54086..55519
                     /gene="rpoN"
                     /locus_tag="OO160_000517"
     CDS             54086..55519
                     /gene="rpoN"
                     /locus_tag="OO160_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5522760.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55542..55829
                     /gene="hpf"
                     /locus_tag="OO160_000518"
     CDS             55542..55829
                     /gene="hpf"
                     /locus_tag="OO160_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5522761.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55947..56438
                     /gene="ptsN"
                     /locus_tag="OO160_000519"
     CDS             55947..56438
                     /gene="ptsN"
                     /locus_tag="OO160_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5522762.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56484..57338
                     /gene="rapZ"
                     /locus_tag="OO160_000520"
     CDS             56484..57338
                     /gene="rapZ"
                     /locus_tag="OO160_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5522763.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57335..57607
                     /gene="npr"
                     /locus_tag="OO160_000521"
     CDS             57335..57607
                     /gene="npr"
                     /locus_tag="OO160_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5522764.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57820..58452
                     /gene="yrbL"
                     /locus_tag="OO160_000522"
     CDS             57820..58452
                     /gene="yrbL"
                     /locus_tag="OO160_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5522765.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58449..59176)
                     /gene="mtgA"
                     /locus_tag="OO160_000523"
                     /pseudo
     CDS             complement(58449..59176)
                     /gene="mtgA"
                     /locus_tag="OO160_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59173..59826)
                     /gene="elbB"
                     /locus_tag="OO160_000524"
     CDS             complement(59173..59826)
                     /gene="elbB"
                     /locus_tag="OO160_000524"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5522766.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60056..62392)
                     /gene="arcB"
                     /locus_tag="OO160_000525"
     CDS             complement(60056..62392)
                     /gene="arcB"
                     /locus_tag="OO160_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5522767.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62488..63417)
                     /locus_tag="OO160_000526"
     CDS             complement(62488..63417)
                     /locus_tag="OO160_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5522768.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64092..68552
                     /gene="gltB"
                     /locus_tag="OO160_000527"
     CDS             64092..68552
                     /gene="gltB"
                     /locus_tag="OO160_000527"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5522769.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68565..69983
                     /gene="gltD"
                     /locus_tag="OO160_000528"
     CDS             68565..69983
                     /gene="gltD"
                     /locus_tag="OO160_000528"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5522770.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70167..>70449
                     /locus_tag="OO160_000529"
     CDS             70167..>70449
                     /locus_tag="OO160_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522771.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFY"
BASE COUNT        17243 a        19113 c        17505 g        16588 t
ORIGIN      
        1 actgctggat gatggtcgcc tgagctatag cctggaacaa agtctggatg acgataacaa
       61 ccataacagt agcctgaacg ccagttaccg ttcaccttat ggaaccttca gtgccggata
      121 cagttacggt aatgacagca gccaatacaa ttacggcgtt accggcggcg tggttatcca
      181 tcctcatggc gtgacgctct cgcaatatct gggcaacgct tttgcgctta tcgatgctaa
      241 cggggcttct ggcgtgagga tacaaaacta tccggggatt gctactgatc cctttggcta
      301 tgcagtggtt ccttatctca cgacttatca ggaaaaccgt ctctcggtag atactacgca
      361 gctgcccgat aacgtcgatc ttgaacaaac aacacagttt gtggtgccca acagaggtgc
      421 aatggtagcg gcgcgtttca acgccaatat cggttatcgc gtacttgtta cagtcagcga
      481 tcgcaacggt aaaccgttgc cctttggcgc tcttgccagc aacgatgata cggggcaaca
      541 aagtatcgtc gatgagggcg gcatactata tctctctggg atatcgagta aatcacaaag
      601 ctggactgta cgctggggaa atcaggcaga tcaacaatgt cagtttgctt ttagtacacc
      661 ggattcagaa ccaacaacct ctgtattaca aggcacagcg cagtgccatt aaggataaaa
      721 aaatgaaaag agcgcctctt ataacaggac ttttgttgat atccacatcc tgcgcttatg
      781 cctcctcagg agggtgtgga gccgacagca ctagcggtgc gacaaattac agcagtgtgg
      841 ttgatgatgt tacggtgaac cagacagata acgtgacagg acgggagttt acctctgcaa
      901 cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag gcagttaaac
      961 ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga tattacaaac
     1021 tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca ggactcacaa
     1081 ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc accgtatatt
     1141 ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga cccgttaata
     1201 ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc tcgatgacca
     1261 ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg ggaagcccgt
     1321 ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc accgccccac
     1381 agtcgtgcaa aattaatcag ggcgatgtta ttaaagttaa ttttggattc atcaatggtc
     1441 agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac tttgatatca
     1501 cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc atcgacggta
     1561 caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca gacaatgtgc
     1621 ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt ccttttcaga
     1681 acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc gcctggccag
     1741 ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcacagcc accattaccg
     1801 tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg gcactgttac
     1861 ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg aatttctgcg
     1921 gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag cgtgcgcagg
     1981 gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag caccatttcg
     2041 cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac gggcgcgccg
     2101 tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg ggattcgcct
     2161 aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc ataaaaaatc
     2221 agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt tgatttggca
     2281 ggtaaaaagc cttcgtaaca gaaacggtca gatggtaaac ccgcggcgct taacgcagtg
     2341 atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg gcaggtacgc
     2401 accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac cagcgcaatg
     2461 ttttgcccct cttgcagctt tgccagcagc gtttcggctt tttgttgttc gttatggtcg
     2521 tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc ggtgtgacga
     2581 gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgtacg ctgggtgata
     2641 tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga attatccgcc
     2701 gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc attgcgtaaa
     2761 aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa agccgcgcgt
     2821 tgtctgcctg ttgttctggc agccctgatt ttcgccggtt gtggcaccca tactcccgat
     2881 cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta tcttcagcag
     2941 atgcagcaaa gctctgatga taccaggatc aactggcaat tactcgccat tcgtgcactg
     3001 gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc gcaagaactg
     3061 aacgattctc agcgtcgcga gaaaacactg ctggcggcag agattaaact ggcgcagaaa
     3121 gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt agaacaaaac
     3181 cagcaagcgc gttactggca ggcaaaaatc gatgccagcc aggggcgtcc ttccattgat
     3241 ttactgcgcg cgttaattgc tcaggaaccg ctgcttggcg cgaaagaaaa acagcagaat
     3301 attgatgcca cctggcaggc gctctcctcc atgactcagg aacaggcgaa tacgctggtg
     3361 atcaacgccg acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt ctggtttgat
     3421 aaccgtaacg atcccgacat gatgaaagcc gggatcgccg actggcagaa acgttatccg
     3481 aacaatccgg gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc gtttaaacca
     3541 gcctcgacca acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc ggtatttggt
     3601 cgcactattc agcaaggctt tgaagccgcg aaaaatatcg gcactcagcc agtggcggct
     3661 caggtagctg ccgcacctgc cgcagacgta gcagaacaac ctcagccgca aactgcggat
     3721 agcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca gcctgcggcc
     3781 cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc acccgcaaat
     3841 ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca gatcttaagc
     3901 caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa taacgttgaa
     3961 gagttgctga agagcaacac cccgctgaac gtactggcac tgaaccagcc ggagaatatc
     4021 gaaaaccgcg tcaatatttg ttacttcgcg ctttcaccgg aagacgaagc gcgcgatgca
     4081 gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc acgcagtgca
     4141 ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc agaaactggg cggcggcacc
     4201 gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa cggcggttct
     4261 ggtattgctt taacgggtag cccgattact cccagagcga caaccgactc cggcatgacg
     4321 accaacaatc caacgctgca aaccacgcca accgatgacc agttcaccaa taatggcggt
     4381 cgtgtcgatg cggtctacat tgtggcaacg ccgggtgaaa tcgcttttat caaaccgatg
     4441 atcgccatgc gtaacggtag ccagagcggt gcaacgctgt acgccagttc ccgcagcgca
     4501 caaggcaccg caggcccgga tttccgactg gagatggaag gcttgcagta cagcgaaatc
     4561 ccgatgctgg caggcggtaa tctgccgtta atgcagcagg cgctcagcgc ggtgaacaac
     4621 gattattcgc tggctcgcat gtatgcgatg ggcgttgatg cctggtcgtt ggcaaatcat
     4681 ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg aagtctgacg
     4741 gctaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca acaaggtcag
     4801 gtagtccccg ccagttaacc accaaacaga ccggcgatgc gtgggaagca caagcgcgtc
     4861 gctggctgga aggcaaagga ctgcggttta tcgccgctaa cgtgaacgag cgtggcggcg
     4921 agatcgatct gataatgcgt gaaggcctga ccaccgtttt tgtcgaggta cgctatcgcc
     4981 gctctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa cacaaattat
     5041 tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact gtggattgcc
     5101 ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat gcctttaatg
     5161 accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc ttgcttcact
     5221 gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat ctcccgtgca
     5281 gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg tggtaatgga
     5341 acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt cgaaacggag
     5401 cggcccagct tacctgccat tgcactaaat actgataatg ttgtcttaac ggcgattgcc
     5461 aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg tcatgcggga
     5521 gatgtattgt tagccatttc cacccgtggc aacagccgcg atattgttaa agcagttgaa
     5581 gccgccgtta cgcgtgatat gaccattgtg gcattgaccg gctatgacgg cggcgaactt
     5641 gcaggtttgt tagggccaca ggatgtggag atccgcattc cttcgcatcg tagtgctcgc
     5701 attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga taacacgctt
     5761 ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat cgcagtcctt
     5821 atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac cgctgctgtg
     5881 ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc aggtggacga tggtaccctg
     5941 gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga aacccgtatt
     6001 aatgtcacgg cctatcaggg caaagtgctg ctggttgggc agtcaccaaa tgctgaactt
     6061 tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt gtataacgag
     6121 attcgtcagg gccagccgat tggtctgggc gaagcatcta acgatacgtg gatcaccacc
     6181 aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt gaaagtgacc
     6241 accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc gaaagcggcg
     6301 gcagatattg ccagccgggt gagcggcgta aagcgggtta ccacagcgtt tacgtttatt
     6361 aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca ggcctacaac
     6421 gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt tccctcaaat
     6481 cagaacaaca gcgccagccc gctgacaatc acaccagaca ctgccaccat cgcccctgtc
     6541 agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg caaactcact
     6601 gccgggagcg tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc cagcatcatc
     6661 gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc tgctaccgcc
     6721 atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg gaataaccag
     6781 acgcgtgcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt actccagaaa
     6841 agtgtccata gcgccctgcc ccagcggcta aaaaaaccgc tctgtgcttc cggtatgtca
     6901 atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac cagcgtcgca
     6961 atcagcaaca ccatcaccag cccggccacc agacgaatcg ccgcaaaacc ccagccgagg
     7021 acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat ccagaatgcc
     7081 agcgcaccgc ccatcgatac ctgttggcga cgcattcccg ccgcgaccgg agccgcacag
     7141 caggtacaca tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc gcgaaagcgc
     7201 gattgcccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat caacgaaccg
     7261 aggatcaccc ccagcaccgc cgctttccat actgcgagga aatagatcat cgcgtaatcc
     7321 aacgccgctt gccacggatt agcatccgcc tgcgcaagga tagatttacc gatactgtgg
     7381 gtttcggctg cagtaaacgc tttgccgtag taaggttgcc atttcacata ccagagaccg
     7441 gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt tgccgcctga
     7501 gatgaagact gaccagtcat agcattcccc aggaataagt tatgataatt gagcgcgtga
     7561 atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg taattctgaa
     7621 gagcttaaaa tcgtcacgcc ttcatgctca ggccgcatcg cctctgccag catgacccgc
     7681 gccacgtctc tcgcatcaat ggatttccag ttacctggta acaaacggaa cagcggcgca
     7741 aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gtaacatcga cgggcgagca
     7801 atggtcaatt tcggccagtt ctgggcaatt aatgcttctt ccatctcccc tttgacgcgg
     7861 ttatagaaaa acggcgagtg ggcattggca cccatagcac tgaccaccag catatgctgc
     7921 gcgcccagtc gccgcccggt taatgcggta tccactacca gcgtgtaatc ggcatgaata
     7981 aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg
     8041 atgggttcgg tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg attaaacacc
     8101 ccgggcatat cgcccaacgg acgtcgcgtc ggcgcagcaa tggcgttaac tttcggttca
     8161 ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt aatcagtacc
     8221 tgactcatct tcgttccttt acagaattgt ctgccttgcg ctccccggct catgcaccag
     8281 tcttaataga ccatgaggta attatccccg attgtggaaa attcgcctca tccaatgcaa
     8341 caacgcggag gaagcatgag taagaaaatt gccgttttaa tcactgatga atttgaagat
     8401 tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt gattactatt
     8461 gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt gaccatcgat
     8521 aaatccatcg atgaagtgac gccagcggag tttgatgccc tgttgctacc gggtggtcat
     8581 tcaccggatt atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga ttttgtgaat
     8641 agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag cgccgatgtg
     8701 attcgggggc gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa aaatgcgggc
     8761 gtggaatttt acgatcagga agtcgtggtt gataaagatc agctggttac cagccggaca
     8821 ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc ctgagtcgcg
     8881 tagccaaatc atttttttac caaagcccag cgtgttgtcg gtgaatttga tttcatcgag
     8941 ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgac gattgtacgc
     9001 cttgcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt taaactgcac
     9061 accgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa ccgcagcctg
     9121 cggcccactc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt
     9181 ctgcgcatca aaaagataaa aggcattagc gcaccagagt tccccttcct gctgcacaca
     9241 ccaggtgaca acatgttgtt ttgccagcca acggctgatg gcgatgagtg tttccattgc
     9301 tgttctcttt tatactgtgg gcctgacctt aacatgcgat gcagacgatg acaccctggt
     9361 ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc acggatgtcg
     9421 aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc gggaaaggag
     9481 aactgacgct ggcgttttct gcgccagtgg gcgaccgttc gctggcacta cgggcggaat
     9541 atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa ggcgcagggt
     9601 ttgcagaact gttgagcagc ctgcaaaccc cggagattaa aagcgattga aatgctcgtg
     9661 atactcaacc agaccggtta cgccattcag cgcgtcatcc gctaaacgat gtaccaggaa
     9721 ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca gttcaaagcc
     9781 gaaacgactg tacagcgccg gatcgcccag cgtcaccact gcggcatagc cgaactcatt
     9841 aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac ggtatttttc
     9901 atcaaccgcc aacggtgcca tgccgaccca ttgcaggtct tcgccctgca catcaaccgg
     9961 actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca gccccagcgt
    10021 cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac tttcgaatga
    10081 gcgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc gaattagcat
    10141 ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc ctcaacaaaa
    10201 tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag ttcaatggcg
    10261 tccatcaggt ttttcacata cacccccagc tccgtatcgc cttcaatcac cagccgacgc
    10321 tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag atcgctggcg
    10381 tcggcactaa aactcacatc agcttgcgtg ttctggctga ccaccagttt gccattcacc
    10441 accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca gcggccttca
    10501 agaaactcca gctcgccatc atccagcgcc tgacggaatt gccagcttaa gacctgctca
    10561 agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa cagagatggc
    10621 cccaaatgca caatacggga acgcagttta tccaacacga gttttactcc ctgtttcaac
    10681 aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa caattccata
    10741 tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat tgtcgcagca
    10801 aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgatagcgca accttcagga
    10861 aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa
    10921 aacggcgcag atgctgttta tatcgggcta aaagatgata ccaatgcccg tcacttcgcc
    10981 ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca tcaacatcgc
    11041 cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc ccgttggcag
    11101 cgtgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc cgacctcgcc
    11161 atgctggagt acgccgccga gcgttacccg catattgaac gtcatgtgtc agtgcaggct
    11221 tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgacgt tgcccgcgtg
    11281 gtgctgccgc gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt tactcctgtc
    11341 ccgctggaag tgtttgcttt cggcagcctg tgtattatgt cggaaggccg ttgctatctg
    11401 tcgtcgtatc tgacgggtga gtcgcccaac actgtgggtg cgtgttctcc ggcccgtttc
    11461 gtgcgctggc aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt gctgatcgac
    11521 cgttatcagg acggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg ttatctggtg
    11581 gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct ggaactgctg
    11641 ccggagttaa tggctgcgaa tattgcttcg gtgaaaattg aaggccgcca gcgtagcccg
    11701 gcgtatgtca gccaggcggc gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat
    11761 ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc
    11821 acgcaaacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca atgaaatatt
    11881 ccttagggcc agtgctgtgg tactggccaa aagagacgct ggaagatttt tatcagcaag
    11941 ccgccgccag cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc aagcgccggg
    12001 caaccaaagt tggcgactgt ctggagatgg caaaatcgct cgccggaagt ggcaagcaga
    12061 ttgtgctctc cacgctggcg ctggtgcagg catcctctga actgggcgaa ctgaaacgct
    12121 atgttgagaa cggtgaattt ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg
    12181 ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa ctgctacaac gcggtgacgc
    12241 tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag ctttcccgcg
    12301 actggctggt gaatctgctt aatcagtgcg atgagctggg cattcgcaac cagtttgaag
    12361 tggaagtcct gagctacggt catctaccgc tggcctactc cgcccgctgc tttaccgcgc
    12421 gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat ccgaacgggc
    12481 gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt cagaccatga
    12541 gcggctacgt ttacaacctc ggtaacgagc tggcatccat gcagggattg gttgatgtag
    12601 tccgcctgtc accgcagggc actgacactt tcgcgatgct cgacgccttc cgcgctaatg
    12661 aaaatggcgc tgtgccactg ccgttgacgg caaacagcga ctgtaacggc tactggcggc
    12721 ggctggcagg actggagctg caagcctaag taaatagctc actttgttaa caactttaac
    12781 tattctttaa tgcagtatta aagattaatc ggtaacaaag tgagctgtta tgactgataa
    12841 aaccattgcg ttttcgctac tcgatctggc ccccataccc gaaggctctt cagcgcggga
    12901 agcgttctcc cactctctcg atctcgcccg tctggctgaa aagcgcggct atcatcgcta
    12961 ctggctggca gaacaccaca atatgaccgg cattgccagt gctgcaacat cggtattgat
    13021 tggctatctg gcggcgaata ccaccacgct gcatctgggg tctggcggcg tgatgttgcc
    13081 taaccactta ccgttggtca ttgccgaaca gttcggcaca ctcaatacgc tctatccggg
    13141 gcgaatcgat ttggggctgg gtcgtgctcc gggtagtgac caacggacaa tgatggcgct
    13201 acgtcgtcat atgagcggcg atattgataa tttcccccgc gatgtcgcgg agctggtgga
    13261 ctggtttgac gcccgcgatc ccaatccgca tgtgcgcccg gtaccaggct atggcgagaa
    13321 aatccccgtg tggttgttag gctccagcct ttacagtgca caactggcgg cgcagcttgg
    13381 tctgccattt gcgtttgcct cacacttcgc gccggatatg ttgttccagg cgctgcatct
    13441 ttatcgcagt aacttcaaac cgtcggctcg gctggaaaaa ccatacgcga tggtgtgcat
    13501 caatattatc gccgccgaca gcaaccgcga cgctgaattt ctgtttacct caatgcagca
    13561 agcctttgtg aagctgcgcc gtggcgaaac cgggcaactg ccgccgccga ttcaaaatat
    13621 ggatcagttc tggtcaccgt ctgagcagta tgacgtgcag caggcgctga gtatgtcgtt
    13681 ggtaggcgat aaagcgaaag tgcgtcatgg tttgcagtcg atcctgcgcg aaaccgacgc
    13741 tgatgagatt atggttaacg ggcagatttt cgaccaccag gcgcgcctgc attcgtttga
    13801 gctggcgatg gatgttaagg aagagttgtt gggatagtgt gtcttaacgc gggaagcctt
    13861 atccgagctg gcaacgctgt cctacgtagg cctgataagc gaagcgcatc aggcattgtg
    13921 taggcagcag aaatgtcgga taaggcaccg ctgattactg atacaccggc agtaaattaa
    13981 agctcgataa aatatgcacc agtgcgttgc cgacgccaaa caccagaatc agcgcaatca
    14041 tcggcttgcc accccagacg cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg
    14101 ccagcagtgc cggaacaatt gccgcccaga tggtagccgc taaaccagca taaccaatgg
    14161 catacaggaa tccgttcggg aacagcagac cacccacaac aggcggggca aaggtcagca
    14221 atgccgtttt caagcggccc acagccgagt cgtcgaaacc aaacagatct gccagatagt
    14281 caaacaaacc cagcgttacg ccaaggaacg aactcgctac cgcaaagttt gagaacacga
    14341 ccagcagaag gtccaggcta cggctgttca gcacgccgct taatgcttgt accaacacat
    14401 caatattacc gcccttctcg gcgatgccga taaactccgg acgagggatg ttgcccattg
    14461 tcgccagcaa ccagatggta tacagcgcca gtgccatcag cgtaccgtac accaggcatt
    14521 tcacgatggt tttcggatct ttgccgtaat acttcatcag gcttggcacg ttaccgtgat
    14581 aaccaaacga cgccaggcag aacggcaggg tcatcagcaa atacggcgca taagatgcat
    14641 tgctttcggc aacgttgaac aatgtcgcag gctgcacatg ccccagcagg ctaccgaagg
    14701 tgaggaagaa ggtaatgact ttcgccccca gcacaatcgc cgtcatgcgg ctgacggctt
    14761 tagtactcaa ccacaccaca aacgctacca gcagtgcaaa accaaaaccc gccgcccgtg
    14821 ccgggacgtt tagcgacatc tctgcgaagg tgtgatgcag aatcgaacca ctggcagaaa
    14881 tataggcata ggtcaggata tagagcacaa aggcaatgga aatgccgttg accacgttcc
    14941 agcctttgcc cagcaaatct ttggtgatgg tgtcaaaact cgaaccgatt cggtaattca
    15001 ggttagcttc cagaatcatc aagccggaat gcagcataca gaaccaggta aagatcagcg
    15061 ctgccattga ccagaaaaac cacgccccgg acatgaccac tggcagagaa aacatccctg
    15121 cgccaataat ggtgccgccg ataatcacca cgccgccaag cagcgacggt gacgtttggg
    15181 tggtggttag tgttgccatg agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt
    15241 atgcattgca ctgtaccagt acatgagtac aaaagacaga aaaaaagccc cgatggttaa
    15301 aatcggggct gtatatatta ttttacagat tgtgttcgct gttcagcgat gattacgcat
    15361 caccaccgaa acgacgacga ccggtagagt catcacgacg cggtgcgcgg ccttcacgac
    15421 gttcgccgct aaaacgacga ccatcaccac gaccaccttc acggcgttca ccgctgaagt
    15481 tacgaccgcc ttcacgacgc tcgccaccga aaccacgacc accgccacga cgctcaccgc
    15541 cagtatgcgg ctgtgcatcg cccagtaact gcatgttcat cggcttgttg agaatgcgag
    15601 tgcgcgtaaa gtgttgcagc acttcacccg gcataccttt cggcagttcg atggtggagt
    15661 gagaagcaaa cagcttgatg ttaccgatgt aacggctgct gatgtcgcct tcgttagcaa
    15721 tcgcgccaac gatatgacga acttcaacac catcatcgcg gcccacttca atgcggtaca
    15781 gctgcatatc gccaacatca cgacgttcac gacgcggacg atcttcacgg tcgccacgcg
    15841 ggccacggtc gttacgatcg cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg
    15901 gacgcatcgg cgcatctggc ggaacaatca gagtacgttc accctgtgcc attttcagca
    15961 gtgccgcagc cagagtttcg agatccagct cttcaccttc tgcagtcggc tgaattttgc
    16021 tcagcagtgc gcggtattga tccagatcgc tgctttccag ctgctgctgt actttagcgg
    16081 cgaatttttc cagacggcgt ttgcccagca gttctgcgtt cggcagttct acttccggaa
    16141 tagtcagctt catagtacgt tcaatgttgc gcagcagacg acgctcgcgg ttctcaacga
    16201 acagcagcgc gcggccagca cgacccgcac gaccggtacg accgatacgg tgaacgtaag
    16261 actcagaatc catcgggata tcgtagttaa ccaccaggct gatacgctca acgtccaggc
    16321 cacgggccgc aacgtcggtc gcaatcagga tgtccagacg accgtcttta aggcgttcca
    16381 gtgtctgctc acgcagcgcc tggttcatgt caccgttcag cgcggcgcta ttgtagccgt
    16441 tacgctcaag agcttcagcc acttccagag tcgcgttttt ggtacgaacg aagataatcg
    16501 ccgcatcaaa atcttccgct tccaggaaac gtaccagtgc ttcgttttta cgcatacccc
    16561 acacagtcca gtagctctgg ctgatgtcag gacgggtagt cacgctggac tgaatacgca
    16621 cttcctgcgg ctctttcata aagcggcggg taatgcgacg aatcgcttcc ggcatggttg
    16681 cagagaacag agcggtctga tgaccttccg ggatctgcgc cataatggtt tcaacgtctt
    16741 cgatgaagcc catgcgcagc atttcgtcag cttcatccag aaccagaccg ctcagtttag
    16801 agaggtccag agtgccacgt ttcaggtggt ccagcagacg gcccggagta ccgacaacga
    16861 tctgcggccc ctgacgcagg gcgcgtaatt gcacgtcata acgctggccg ccgtacaggg
    16921 caaccacatt tacgccgcgc atgtgtttag agaaatccgt cattgcttca gcaacctgta
    16981 ccgccagttc gcgggtcggt gccagcacca gaatctgtgg tgctttcagc tcaggatcaa
    17041 gattctgcag cagaggtaaa gagaatgctg cagtttttcc gctccccgtc tgggccatac
    17101 ccagaacgtc gcggccattc agcagatgtg gaatacactc tgcctgaatt ggagatggtt
    17161 tttcgtaacc cagatcgtta agggcttcaa ggataggagc cttcaggccc agatctgcaa
    17221 aagtggtttc gaattcagcc atgtagtacg tgtgcctcaa aattaatggc ggccagtcta
    17281 cataactcat catgaaattg atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt
    17341 aggtgtatta acgaacaaca acgccctcac ccgttaaggt gatggcaatc aaaaaagatt
    17401 acgggctgat gtgtacgtca gctattgctg gtccgattct gccaggtcat cttggtcctg
    17461 gcccaggagc gataattcca acaatgcgta tcggtgctca acaaagttat gaacgttgtt
    17521 ggcaaccgcc agtttgaaca gtgccgtggc gctgtccaaa tcccccagac ttaggtagta
    17581 cttacctaaa tagaagttgg tttcactgag atgctcagcg agcgaggtgt tatccgttgc
    17641 gtccgccttg agcctttcca ttaacgtttg ttcgctaatg ttgcccaggt agaactcgac
    17701 aatgttccat ccccactgtt ccttatccga tttttcgaag tgctgtttca atacttcttt
    17761 agcctgcttc tcatcgagct tctgctcggc gagataaagc cacagactac ggaaaggatc
    17821 attgggatcg tcttgataaa acgccagcag atcatcttgc gctaacttgt cacgaccgcc
    17881 gtaatataat gcaatcccgc gattcaagtg cgcgtagttg taagttggat caagctcaag
    17941 tacagaatca aacgcttcat aggcagcatc aaaattgcct gcctgcgtta aatatatgcc
    18001 taagtaattg aatacttcag gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt
    18061 acgcgctaat gccctcagac cgagactatc atacaacact ccgcgctcat ataaaagctg
    18121 tgcgcgttcg tcatcggtta aagcccgact ggcaaggatt tgttccatac gtgccagaat
    18181 cacttcctgc tgtaaagtcg gttgcaatgg taccgcgagg acttcacttt tacgccagga
    18241 agtattactg catcctgcaa gcgtaagtgc tgtcgcaacg aaacaccagc gcaaaaaagg
    18301 cttcatttcc cactcccgaa gaccacggtt gaatgaacgt cctgttcccg gttgctaaca
    18361 aggcgtcctg cccggttaaa agccccccgc cgcagcggag ggcaaatggc aaccttactc
    18421 gccctgttca gcagccggag cttccggtgc tgcagcaggt tgagactgct cagtcgcttc
    18481 tttaatgctc agacggatac ggccctggcg atcaacttcc agaactttca ccggtacttc
    18541 ctgacccatc tgcaggtaat cggtcacttt ctcaacgcgt ttgtcagcga tttgagagat
    18601 gtgtaccaga ccttctttac cgccgccgat ggcaacaaat gcgccaaagt caacgatacg
    18661 ggtcacttta ccagtgtaga cgcggcccac ttcgatttct gcagtgatct cttcgatacg
    18721 acgaatagca tgtttcgctt tctcgccgtc ggtcgctgca atcttcacag taccgtcatc
    18781 ttcgatttcg atggtggtgc cagtttcttc ggtcagggca cggataacag agccgccttt
    18841 accgataacg tctttgatct tgtccgggtt gatcttgatg gtatggatac gtggtgcgaa
    18901 ctcagagata tcgccacgcg gcgcgttgat cgcctgttcc attacgccca ggatatgcag
    18961 acgcgcacct ttagcctggt tcagcgcaac ctgcatgatc tctttggtga taccttcaat
    19021 tttgatatcc atctgcaatg cagagatacc ctcgcgggaa cccgcaactt tgaagtccat
    19081 atcgcccagg tgatcttcgt cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc
    19141 tttcaccaga cccattgcga tacccgcaac ggcagctttg atcggcacac ctgcgtccat
    19201 cagcgccaga gacgcgccgc acacggaagc catagaagag gaaccgttgg attcagtgat
    19261 ttcagacaca acacgtacgg tgtacgggaa tttgtccata tccggcatga ctgccagcac
    19321 gccgcgcttc gccagacgac cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc
    19381 ggtttcgcct acggagtacg gagggaagtt gtagtggaac aggaaggtat cggtacgttc
    19441 gcccatcagt tcatcaagaa cctgcgcgtc acgagcagta cccagcgttg cggtaaccag
    19501 cgcctgcgtt tcaccacggg tgaacagcgc agaaccgtga gtacgcggca gcacgccagt
    19561 acgcacatcc agaccacgga tcatatcttt ttcacgaccg tcgatacgcg gttcgcctgc
    19621 cagtacgcgg ctacgaacaa cgtttttctc gatcgcgtgc agaatttcac ccagttcgtt
    19681 ttcgtccagg gtttcgtctt cagcaagcag cgtcgcgatg gtttcagatt tgatgacatc
    19741 aacctgcgca taacgctctt gtttgtcggt gatgcggtaa gcatcgctca ggcgagcttc
    19801 agccagtgca gcaacgcgcg cgttcagcgc ttcgtttacc ggctccggct gccagtccca
    19861 acgtggttta ccggcttctt tcaccagttc attgatgttc tgaataacaa cctgctgttg
    19921 ttcatgaccg aacactactg cgcccagcat ctggtcttcg ctcagcagtt cagcttcaga
    19981 ttcaaccatc agtacagcgg cttcagtacc ggcaacaacc agatccagtt tgctctcttt
    20041 caactcgtcc tgagtcgggt tcagtacgta ctggtcattg atgtaaccta cgcgagcagc
    20101 accaatcgga ccattgaacg gaataccaga cagagacagt gctgcggaag caccaatcat
    20161 cgcgacgata tccgggttaa cttgcgggtt aacagaaacc acggtggcga taacctgaac
    20221 ttcgttgacg aagccttccg ggaacagcgg ccgaatcggg cggtcaatca gacgcgcgat
    20281 cagggtttcg ccttcgcttg ggcggccttc acgacggaag aagctacccg ggatacgacc
    20341 agcagcgtag gtacgctcct gatagttaac ggtcagtggg aagaagtcct gacctggttt
    20401 ggcttttttc tggccaacaa cggtaacaaa taccgcggtg tcatccatgc taaccataac
    20461 agcggcagta gcctgacgag ccatcatgcc ggtttccaga gtcacggtgt gttggccgta
    20521 ctggaattta cgaacgatcg gattaagcaa tgtaatatcc tttctctttc ttagacagta
    20581 ccttacggca ctggtgttaa tacccgatct tctgcgcatc ctcgcgacta atgacaaccc
    20641 taacccagct ctatgtgggt aaagcctctc attagccgcg cgaacctctg caacggaaga
    20701 tcattcatag caacaataca ttagtttcca gtgaattgct gccgtcagct tgaaaaaagg
    20761 ggccactcag gccccctttt ctgaaactcg caagaattag cgacgcagac ccaggcgctc
    20821 gatgagctgg gtgtaacgtg ctacgtcttt acgtttcagg tagtcgagca gtttacgacg
    20881 ctgagaaacc atgcgcagca gaccacgacg gctgtggtga tcttttttgt gctctgcaaa
    20941 gtggccctgc aggtggttga tctgtgcagt cagcagtgct acctgaactt cagtagaacc
    21001 ggtgtcgttt gcgtcacgac caaactcaga aacgatttta gctgttgctt cagtacttag
    21061 agacatttta aaactccaaa gtatatagaa tgaaaggacg ccgatctcta attcagcgat
    21121 cccagtgtac gttacgcaaa gtgttaaaca atttacgcga cgttaagcgg cagtattcta
    21181 ctcgtagcga cctgttatcg caagacggtt aacattacgc cgggtattca accaccaggc
    21241 gacgaggcgc aacgcggcct tcatcgtcaa tttcgcccat accgataaat ttgccgttct
    21301 caccttctgt aacgcgaacc agtccttcca gcggcgcacc ggatgtacga actgggttac
    21361 catttttgaa gtaaacagaa gacgttaacg gaagattcac caccgggtag tccgaagctg
    21421 gactgtccat tggcatcagt aatggatcaa gtaactccgc agccggaata tcctgctgtt
    21481 cggcttgttc gaccagttca cgcagatgtt ccagggtcac aatccgttca accggatatt
    21541 tacttaccgc cagacgacgc aggtaaataa catgcgcgcc acaaccgagt ttttcaccca
    21601 ggtcatcaat gatggtgcgg atataagtgc cttttgagca gtgaatttcc agctccagct
    21661 cattgccttc gtggcgaata aacagcaatt cataaacggt aatcgggcgc gcttcacgcg
    21721 gtacttcaat gccctgacgc gcatattcgt acagtttttt accctgatat ttcagtgctg
    21781 aatacatcga agggatctgt tcgatatcgc cacggaaagt atccagcgcc gccgccagtt
    21841 gctctgcgct aaaggttacc ggacgttctt caacgatttg tccgtcggca tcagaagtat
    21901 cggtacgctg tccaagacgc gcaatgaccc gatagcgttt gtcggagtcc agcagatact
    21961 gggaaaactt cgtcgcttcc ccgaggcaaa ttggcaacat gccagttgcc agcgggtcca
    22021 gcgcaccggt atgcccggca cggttggcgt tataaatccg tttcactttt tgcagcgcat
    22081 cattgctgga cataccctgc ggtttatcca gcaacaaaac gccgttaatg tcgcgaccgc
    22141 gacgacgagg acgactcatt agtcctcctt gctgtcgtcc gggttaacac gacgttcttc
    22201 gtcatgtttg accacgctgg tcaccaggtt tgacatgcgc atcccttcaa ccagagagtt
    22261 gtcgtagaag aaggtcagtt ccggcacgat acgcaggcgc atcgctttcc ccagcaggct
    22321 gcggatgaaa ccagaagctt cttgcaacgc tttgatgccc gctttaaccg cgtcttcatc
    22381 tttgtcgttg aggaacgtca catatacttt ggcatacgcc aggtcgcgag acatttcgac
    22441 accggaaacg gtggtcatca tgcccaggcg aggatcttta atttcacgct gcaggatgag
    22501 agcgatctct ttttgcattt cctgcgctac gcgctgcggg cgaccaaatt ctttcgccat
    22561 aataaattct cctgacaaaa aaggggctgt tagccccttt ttaaaattaa tttcaggtgg
    22621 aagggctgtt cacgttggcc tgataagacg cgccagcgtc acatcaggca atccatgccg
    22681 gatgcggcgt aaacgcctta tcccgcatgg aaccctaaaa accttaagcg atggtacgtt
    22741 ggatctcgat gatttcgaat acttcgatca catcgccagt gcggacgtcg ttgtagttct
    22801 taacgccgat accacattcc ataccgttac ggacttcgtt aacgtcatct ttgaagcggc
    22861 gcagggactc cagctcgcct tcgtagataa ccacgttgtc acgcagaacg cggatcgggt
    22921 tgtgacgttt aaccacacct tcggtaacca tacagcctgc gatggcacca aatttcggcg
    22981 atttgaacac gtcacgaact tccgccagac cgataatctg ctgtttcagt tccggagaca
    23041 gcataccgct catcgccgct ttcacttcgt caatcaggtt atagatgacg gagtagtaac
    23101 gcagatccag gctttccgct tcaatcactt tacgtgcaga ggcatcagca cgtacgttga
    23161 agccaaccag gatggcgttg gacgccgcag ccagggtggc gtcggtttcg gtgataccac
    23221 ctacgccaga accgatgatc ttcactttaa cttcgtcagt agacagtttc agcaaggagt
    23281 cggagatcgc ttccacagaa ccctgtacgt ctgccttcag gacgatgttc acttcgtgaa
    23341 cttcgccttc ggtcatgttg gcgaacatgt tctcgagttt agatttctgc tgacgcgcca
    23401 gtttaacttc gcggaattta ccctgacgat acagtgcaac ttcacgcgct ttcttctcgt
    23461 cacgtacaac ggtaacttca tcacccgcag ccggtacgcc ggacaggccg aggatttcca
    23521 ccggaatgga cggacccgct tccagcactt cctgacccag ttcgttacgc atcgcacgaa
    23581 cacgaccgta ttcgaagcca cacagaacga tatcgccctt gtgcagagta ccttcacgta
    23641 ccagaacggt agcaaccgga ccacgacctt tatcgaggaa ggattcgata accgcaccgc
    23701 tcgccatacc tttacgtacc gctttcagct ccagaacttc cgcctgcagc aggatagcgt
    23761 ccagcagttc atcgatacca gtacccgctt tcgcagacac atgtacgaac tggctttcac
    23821 cgccccactc ttccggcagg atgccgtact gggagagttc gttcttaacg cgatccggat
    23881 cggcttctgg tttatcgatc ttgttcactg caacgaccac cggaacctgc gccgctttcg
    23941 cgtgctggat tgcttcgatc gtctgcggca ttacgccgtc gtcggcagca acaaccagga
    24001 ctacgatgtc cgttgcctgc gcaccacgag cacgcattga agtaaacgcg gcgtgccccg
    24061 gggtgtccag gaaggtgatc atgccgtttt cagtttcaac gtggtatgca ccaatgtgct
    24121 gggtaatgcc gcccgcttcg ccagaggcca ctttcgttga acgaatgtag tccagcagag
    24181 aggttttacc gtggtcaacg tgacccatga tggtcacaac cggcgcgcgc ggttcagccg
    24241 cagcacccgt gtcacggtcg ctcattaccg cctcttccag ctcgttttca cgacgcagga
    24301 taactttatg gcccatctct tcagcaacca gctgtgcggt ttcctgatcg ataacctggt
    24361 tgatggttgc cattgcgccc agtttcatca tcgctttgat gacctgagag cctttaaccg
    24421 ccatcttgtt cgccagttcg ccaacggtga tagtttcgcc gatcacaacg tcacggttaa
    24481 cggcctgagc aggcttctgg aagccttgct gcagcgaaga acctttacgt tttccgcctt
    24541 taccgccacg tactgctgcg cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt
    24601 tgcctttctt cggacgcgct gctttcgcgt tacgaccacg gccacggccg ccttcgactt
    24661 cacgatcgct ttcgtcttct gcctggcgag catgttgaga agtagtgacg tgataatcgc
    24721 tggaatcttc agtcggttcc gcgttatcag tccatttgtt ttcttccgcc atacgacgtg
    24781 cttcttcagc aacgcgacgt gcttcttctt cgagtttacg acgcgcttct tcttcagctt
    24841 tacgcttgag ctctgcagct tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag
    24901 tcatatcgtc ttgttgattg ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt
    24961 cagcagcttc acgcttagct tgttcagcag cttcacgctt agcttgttct gcggcctcac
    25021 gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct
    25081 cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc
    25141 ggacttcgat ttgtaccgat ttgctttttc caccggtacc aggaatgtta agggtgctgc
    25201 gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc aggtggtcaa
    25261 tcaaagtctg tttctcttgt gcagacacag agtcgtcagc agacttccgg atacctgcat
    25321 cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa
    25381 tcgttacatc tgtcatgctg ttccttcctg ctacagttta ttacgcttcg tcaccgaacc
    25441 agcaaatatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga
    25501 tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg
    25561 cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg
    25621 gtttgttatc accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag
    25681 cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt
    25741 tcatcggcac ataggccagt tcttccagcg tcgagaaacc ttcttctacc agaacagtcg
    25801 cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg
    25861 cctgatgctt agcctgcagg tcgtcaacgg tcattacgtt gagttcccaa ccgctcagct
    25921 gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg ttaccagctt
    25981 caacggcgat atccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg
    26041 gtgccattgc gttaatcaca aactgcgccg ggttatcatc ccacaggacg atatcgatac
    26101 gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc gccacgcata cctacgcaag
    26161 cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg
    26221 ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttccggc acttcaatac
    26281 ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc gcgccacgcg
    26341 cttccgggcg aacggaataa agcacgccac gaacgcggtc gccagggcgg aagttttcac
    26401 gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga tccagagaga
    26461 tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga
    26521 actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg
    26581 ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc
    26641 ccaagttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct
    26701 gggtgacttc atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat
    26761 cgatctgtac acggacgtcg atctcttgct catatttttt ctttgttgct gtcgccagcg
    26821 cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt
    26881 caactacagc caaaatttct ttgttcatcg cgggcttttc acctcatcca gactattaaa
    26941 agtggggaac caggttcgcc ttctggatat tactcagcgc gaacacttca tctttacctt
    27001 cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc catttacgac
    27061 ggttttgtac cgccatacgg agaaccagag tcacctcttc tccgacaaaa cgggcgtagt
    27121 gttcagccgt gaacagtggg cgatcgagac ccggtgagga gacttccagg ttataagcaa
    27181 cggtgatggg atcttcaaca tccagcacag cacttacctg gtggctcaca tcagcacaat
    27241 catcaacatt gatgccatct tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc
    27301 gaataaattc gatgcctacc agttcaaaac ccagggcctc aactggcgca gtaatcatct
    27361 ctgttaattt ttgctctaat gtggacaagc ccacccccaa gacataaaaa aagggcctaa
    27421 agcccagtta ttctgtagtc agataacaaa aaaccccgat aaatcggggc tttatataac
    27481 tgaaccctat aaccgcaact gcggtctgga gcactttcca gaaggatttt ttcaaatccc
    27541 actacgaagg ccgaagtctt cacagtatat ttgaaaaagg actctaaggg aaagtggttg
    27601 cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc
    27661 tccaccccgc gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact
    27721 gctgggattt ggtgtaccga gacgggacgt aaaatctgca ggcattatag tgatccatgc
    27781 cacattttgt caacgtttat tgctaatcac gtgaatgaat atccagttca ctttcatttg
    27841 ttgaatactt ttgccttccc ctgctctccc ttaagcgcat tattttacaa aaaacacact
    27901 aaactcttcc tgtctccgat aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa
    27961 attcagtgag agcggaaatc caggctcatc atcagttaat taagcagggt gttattttat
    28021 gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg
    28081 tctggacacc agtgccgcac tgctgtggat gcgacaaaag ggagcggttc cttatgcata
    28141 tactgcaaac ctgggccagc cagacgaaga ggattatgat gcgatccctc gtcgtgccat
    28201 ggaatacggc gcggagaacg cacgtctgat cgactgccgc aaacaactgg tggccgaagg
    28261 tattgccgct attcagtgtg gcgcatttca taacaccacc ggcggcctga cctatttcaa
    28321 cacgacgccg ctgggccgcg ccgtgactgg taccatgctg gttgctgcga tgaaagaaga
    28381 tggcgtgaat atctggggtg acggtagcac ctataaagga aacgatatcg aacgtttcta
    28441 tcgttatggt ctgctgacca atgctgaact gcagatttac aaaccgtggc ttgatactga
    28501 ctttattgat gaactgggcg gccgtcatga gatgtctgaa tttatgattg cctgcggttt
    28561 cgactacaaa atgtctgtcg aaaaagccta ctccacagac tccaacatgc ttggtgcaac
    28621 gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca acccgattat
    28681 gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca gaagaagtca cagtacgctt
    28741 tgagcaaggt catccggtgg cgctgaacgg taaaaccttt agcgacgacg tagaaatgat
    28801 gctggaagct aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc agattgagaa
    28861 ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg cactgctgca
    28921 cattgcgtat gaacgtctgt tgaccggtat tcacaacgaa gacaccattg agcagtatca
    28981 cgcgcatggt cgtcagttgg gccgtctgct gtaccagggg cgttggtttg actcccaggc
    29041 gctgatgctg cgtgactctc tgcaacgctg ggttgccagc cagatcactg gtgaagttac
    29101 cctggagctg cgccgtggga acgattattc aatcctgaat accgtctcag agaacctgac
    29161 ctacaagcca gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg
    29221 tattggtcaa ttgaccatgc gtaatctgga tatcactgat acccgagaga aactttttgg
    29281 ttatgccaaa actggcctgc tttcctcctc tgccacttca ggcctgccgc aggtggagaa
    29341 tatggaaaac aaaggccagt aattcgacat caaccctgcc cttcaggggt ggggttacct
    29401 tgcttatcct ctgtaaactc ctttctgagc atcacataat cgaaaacatc atggtttgca
    29461 tccacaaccc gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt
    29521 tccggctttg ttacccccat ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag
    29581 atattgtttt ccgttgtgcg atccacgcca tccccaagaa cggggctata ccagataaac
    29641 atcgggacat gatatgcctg ctggctagcc tccctgcctc catgaaaata gacgttcttc
    29701 ttcgtagggt cacgttccag gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct
    29761 tttaataatt caaaaacctg acctagcaaa ctatctgtgt aatgaatgga gttgtcatag
    29821 caggcatcct gatcgtcctg cggttgaaac acggcgctgg attgcggata ggcgctacaa
    29881 gccggttcat ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc
    29941 tgtaatgctt gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt
    30001 tccatggcgc gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc
    30061 gagcttagcc agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg
    30121 taattatgaa tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc
    30181 gcagtgtaag gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct
    30241 tcaacttgcg gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca
    30301 gactccccca caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa
    30361 tacggcaccg tgtttgcaat tgagagtaat ctttgatgcg ctttcgctgc taaagcaaaa
    30421 tagttgagat tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg
    30481 aacgcttttt tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc
    30541 gaaatgacaa tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat
    30601 tttattatta ctgccaaaaa aagaagggat aaaaaagcaa aagcacatag ataagggata
    30661 tacatcccca gcattttcac aacttcatcc ggatcactct gcagcacact aatcgcaaat
    30721 ccatcattga aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc
    30781 gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt
    30841 ttaacaacca ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgctccggaa
    30901 gaggcgacta agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat
    30961 ttaaaatctt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    31021 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    31081 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcgaccatt gcgttctcag
    31141 taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt
    31201 tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa
    31261 ctggagttga cacaataaag tgccaattat gtcagcagta gggaaaagtt tataactaag
    31321 gcgtaccggc accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca
    31381 gatacctgac aaccattcat cagaccagga ccaaaacgaa aagagacgct tttcagcgtc
    31441 tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta
    31501 ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt
    31561 tcgggatatc gctggtcggc ttagccggag cagctggctg agtttgttcg gttttcgccg
    31621 gtgcactcag attttcccat tcgctacctc tattggtttt gttgctattg atgttaccca
    31681 gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt
    31741 taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca
    31801 tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa
    31861 ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta
    31921 ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat
    31981 actaaccaaa acgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa
    32041 aaaaacagca aaaccgatgt gttcgccgag aaaaaaggcg acaatcgccg cctttttagc
    32101 cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt
    32161 cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt
    32221 acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt
    32281 aactgactca tgctcaagcg gatcgccgct acctgcggtg taacgtacgt taaccagaat
    32341 ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat
    32401 cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtggttt tatccagcag
    32461 gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag
    32521 tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag
    32581 ttcaagcccc atgttgctca tcaatgtacc cacagcacca ccacgcagct ggccctgacg
    32641 aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg
    32701 gtcaaccata atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc
    32761 agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg cattgatgtt
    32821 tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt
    32881 cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag
    32941 gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac
    33001 gatacggctg gctttaccca gttcagccga atcaacgcag ctgatctcct tttccatttc
    33061 cgcttcgatg gcctcttcta ccgcatccgg cagtttagtg ccgtcgatag agaagaattt
    33121 aatcccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg
    33181 gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga
    33241 aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg
    33301 cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc
    33361 ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat
    33421 cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag
    33481 tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt
    33541 gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg
    33601 gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca
    33661 ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt
    33721 taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc
    33781 aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg
    33841 gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg
    33901 tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt
    33961 gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc
    34021 agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt
    34081 caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg
    34141 cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc
    34201 caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa
    34261 ggtccagtga agtaccctgg gcaaagagtt tcattatgtt atccctggta tgaattgata
    34321 agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg
    34381 aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc
    34441 atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg
    34501 ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg
    34561 tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt
    34621 gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg
    34681 gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag
    34741 gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca
    34801 ctgagtcacc atgttacgtg ccaggttggt cgcaacttta atatcgttgg acgcaccggt
    34861 agaaacatgt tccggcccgt agatgatctc ttctgccaga cgaccaccgt acagcgtaga
    34921 aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa
    34981 agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc
    35041 cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg
    35101 cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt
    35161 ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc
    35221 gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacgag caatgattgc
    35281 cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga actttcagga tctgctcacg
    35341 accgcgaaca tctggcaagc cgaccacaac ctggcggtcg aaacggccag gacgcagcag
    35401 ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc
    35461 ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc
    35521 accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat
    35581 gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc
    35641 gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc
    35701 tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc
    35761 tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag
    35821 ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat
    35881 ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc
    35941 gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa
    36001 ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt
    36061 gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt
    36121 ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg
    36181 aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga
    36241 ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag
    36301 tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa
    36361 cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta
    36421 gggtttacgc ccggtcgcta caatatacac ttctcgcgaa cgtgcacgag aagagtccgg
    36481 cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc
    36541 ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat
    36601 ttctagcgcc agttccacca gatacatggc tcgggggata tccaccgccg gtgttccgct
    36661 catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag
    36721 cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat
    36781 aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac
    36841 cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt
    36901 aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaatcc
    36961 ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga
    37021 gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc
    37081 aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc
    37141 cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc
    37201 acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga
    37261 ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga
    37321 aaatcgccac cgaagatcgc gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa
    37381 ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaag
    37441 aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa
    37501 aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg
    37561 ccgcagagcg gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt
    37621 cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga
    37681 ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa
    37741 tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt
    37801 cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca
    37861 ttttggtgac atcaatcacc tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc
    37921 cttcgcagaa gccctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc
    37981 catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa
    38041 aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt
    38101 gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct
    38161 gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc
    38221 caggtcagct acaattcaca ttttgatggt cattttaccc tgaagttccc gaagggtcat
    38281 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    38341 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    38401 aggccgcgaa tgttgatgag tacgttactc aactccccgc aggtgccaac cttgccctga
    38461 tggtgcaaaa agtcggtgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    38521 tgcccgccag tacccagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg
    38581 attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    38641 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata
    38701 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    38761 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    38821 gctttagcgc tccgcctgct gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    38881 acagtgcccc aaagcctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    38941 cgatgttcag ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgaac
    39001 tggatgtggt gccaagcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt
    39061 ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc ggtgcaattc
    39121 tgaaagatga gttaaaacag gcgggtatca cctggagcgg aacactgctg cgccagactc
    39181 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc
    39241 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatcg
    39301 gccatgcgcg cttcaatgtg cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga
    39361 tcctgcgcca gcaagccggt gtcgatattg gtaacaccat tattgccgat ggttcagggc
    39421 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    39481 aacacgacaa tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt
    39541 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg
    39601 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    39661 tggcatttgt acaatatctt tctggctatg cagtaaaacc cgcggatcag cgtaatcgcc
    39721 gtattccgtt agtgcgtttt gaaagccgtt tgtacaaaga tatttatcag aacaattagt
    39781 caaaaagaaa ccccggcgca tggccggggt ttcagattat tgacaaagtg cgctttgttt
    39841 atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt
    39901 gcaatcatct tgtaggcctg ataagtgtag cgcatcaggc ttatttggca ttggtcatta
    39961 gtgattaacg cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat
    40021 cccagtcttc atcatcctct tcagcaatct cttcaagctg ctgacggtga taatcatccc
    40081 acatgaattc gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa
    40141 tgataaaggt catcacatcc cagcagagat ctttcacgcc cagtccgctc gccgcagaga
    40201 tcagataata tttatcttcc cagcccagcg cttcagcgat cgctttcgct ttctcttcgg
    40261 cttctgcctt atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca
    40321 gatcctggct gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg
    40381 tgccgtcaat cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct
    40441 tcaggaagcg aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat
    40501 cggcaacaac gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag
    40561 tggtaaacgg ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg
    40621 atttacccgc gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca
    40681 gcagctcacg cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg
    40741 acgatttgaa acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct
    40801 gaccgtgttt agtcatatca cccatggttt caccagtacc ctggtcgatt acacgcgtac
    40861 ctaccggcac tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc
    40921 cgttctgacc gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca
    40981 ggttctcgtc ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc
    41041 cgcctttcgg aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg
    41101 caacgaccag aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc
    41161 ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac
    41221 caatggcgga atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt
    41281 ttaacatcgg tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca
    41341 gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca
    41401 tcgcttccgc caaggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg
    41461 cgagctgcca gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcga
    41521 ttgtacataa agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa
    41581 ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagataactc
    41641 cccaggtgta atcggtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca
    41701 ccagaccgct caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca
    41761 ggataaatac gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag
    41821 cggtcggact caggtattgc aaggatgagc tgaacaggat gaagttccca aacagcccgg
    41881 cggtcgccac tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca
    41941 acctcttctt caccgcaaga atggcaccaa ggccaatact cgccatcaag aaacggtaaa
    42001 acacgattgt cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc
    42061 cccagcaaat tgctgtggta agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac
    42121 cgtattccct gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt
    42181 atcgaatgta aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa
    42241 aaacttattc agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt
    42301 tcactttacc gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc
    42361 cagcgtggaa tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc
    42421 caccgaaacg cttaacgccc aagcgtttag cttctgaatc gcgaccgtta cgtgtggagc
    42481 cgccagcctt tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt
    42541 aattttcaca tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg
    42601 acgaaactta acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat
    42661 tacgccgcca tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag
    42721 cacttcagcg aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt
    42781 ctgaccttcg cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa
    42841 ctccgcttcc gcgcacacct tttcaatgat tcagagtgcg ctataaatat tcacaatagg
    42901 gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa
    42961 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa ttaccctata
    43021 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    43081 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    43141 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca actgatcaat cagttaggct
    43201 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    43261 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    43321 acacggcgac tctgctacac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    43381 ctaccgccaa cgccgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    43441 cccgcgcgtt ccagatgatg accagcctcg gttcactcaa agtgctggaa gtcatgtcag
    43501 aagctgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    43561 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    43621 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaggaggag aaaggcctgc
    43681 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    43741 atgccgatgg cgaacagtta ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga
    43801 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    43861 ccgccatcga acaaggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    43921 cttgtggatc tcttgaatgg acgcgtcagc gcgccgagga agaagcagac aaagccatcg
    43981 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    44041 tcgctgttca acgcgatcgt taatcccctc ccttcatccc gcgcagtgcg cgggatgatt
    44101 ccagtaaatt ccataaaaac actaattcag cgctataaca cctgaaaaaa gggcgatgaa
    44161 tctttatata ttctgaatat tcacactctt tacaggaact ttttagcgca atagaccatc
    44221 aggagtatag tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    44281 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg
    44341 cgcaagaaag gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg
    44401 ctggcaaacg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    44461 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    44521 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    44581 tagccccgcg aacggggctg ccagctctca gacgattact cgcctttcac acgctcaata
    44641 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    44701 atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat
    44761 gcacgcagat cggttgccat aacctgtgca ccagaaagtt tttcaacacc atgacaaata
    44821 acggtattgc tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg cacatgcata
    44881 aagcggtttt caaagaccgt ttcggtgatg aacccggtcc cttcagccac caggttcaac
    44941 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    45001 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    45061 tccgctccag catcacgcag tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta
    45121 cggcagataa ttttgccacg agagatcgcc gccgccacca ggaaagtacc ggtttcgata
    45181 cgatccggca gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    45241 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    45301 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc cagggttgca
    45361 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    45421 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    45481 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    45541 ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagcgc ccagatagaa
    45601 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    45661 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag tttcattgat
    45721 gtatcgacgt ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtagt
    45781 gcggcaaaaa gaataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    45841 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    45901 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    45961 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    46021 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatacc gtcaaacaac
    46081 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    46141 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    46201 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    46261 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    46321 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    46381 ttcgcgaggg tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt
    46441 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    46501 cggctaagat cgatgcaggt aatccccttc accgcctcct cacgcatttc ccaaagcggt
    46561 agcagaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    46621 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    46681 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    46741 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcttgc cagtttcccg
    46801 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa
    46861 taatggtaac gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    46921 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    46981 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    47041 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    47101 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    47161 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    47221 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    47281 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    47341 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    47401 caatgagatc ttccagcacc atcgcagact tggtgtcctg aattgtatcg ccatccttca
    47461 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttctc
    47521 ccagcaggcc ggaagtacga atgctcagcg aactggtatc aggaatgtgg ttataacgtt
    47581 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    47641 cccggcccac aacaacacca ccaatgctga ccggagagcg cgctttcagg ccgccaatgt
    47701 tatcgaacgt cgcataaagc gtgtaggtcg attcagtacg tatggacgtc acgttcgccg
    47761 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    47821 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    47881 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    47941 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    48001 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    48061 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagcgat
    48121 ccgccccaga tccccacggc gacgaagata accgtcaaca gtggtaatga aataacccca
    48181 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    48241 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttaacgccga accagcacgc
    48301 ccggcaaaca acagcgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    48361 agcataccca gactggtttc cgcgctataa gtggtcagaa ccagataacc ttgcagcccc
    48421 aacaccattc cgatgaacac gccagaaact acaataatca gcatcgacag gacgccgaca
    48481 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    48541 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    48601 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    48661 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    48721 ctgacgtacg cgcggatcag gattcgtctg caacgcctgg gcactgccat gagcgacaat
    48781 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    48841 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    48901 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    48961 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    49021 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    49081 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    49141 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttacgcac
    49201 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    49261 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    49321 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    49381 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    49441 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    49501 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    49561 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    49621 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    49681 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    49741 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    49801 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    49861 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    49921 cgctggttcg tccttttacc gtccattccg atgttctgcg ccgtgaatta cccttaatgt
    49981 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgaagcgatg
    50041 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    50101 aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc
    50161 ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg
    50221 tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatggctc
    50281 tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata gcgggggttc
    50341 gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg
    50401 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca
    50461 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    50521 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    50581 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    50641 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    50701 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    50761 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    50821 atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat
    50881 cctagtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    50941 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    51001 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    51061 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    51121 agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    51181 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    51241 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    51301 gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt
    51361 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    51421 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    51481 cgtgtgcgcc ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    51541 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    51601 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    51661 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc
    51721 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    51781 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    51841 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    51901 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    51961 gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc
    52021 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc
    52081 cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc
    52141 gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    52201 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat
    52261 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    52321 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    52381 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    52441 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    52501 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc
    52561 acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    52621 gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    52681 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    52741 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    52801 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    52861 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    52921 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    52981 gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg ttctaccaga
    53041 tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa
    53101 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    53161 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    53221 agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga
    53281 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    53341 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    53401 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg
    53461 taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    53521 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt
    53581 tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt
    53641 ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc
    53701 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    53761 gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc
    53821 cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg
    53881 tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    53941 tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg
    54001 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    54061 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    54121 actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga
    54181 acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac
    54241 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc
    54301 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    54361 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta
    54421 ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc
    54481 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgaaaccgg
    54541 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat
    54601 cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gacccggtcg gtgtagcggc
    54661 aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga ccacgccgtg
    54721 gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca atcacgactt
    54781 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    54841 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    54901 cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga
    54961 cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    55021 cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    55081 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    55141 agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca
    55201 ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    55261 tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg
    55321 cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg cggcggaaaa
    55381 cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    55441 ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    55501 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    55561 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa tttgccaaac
    55621 ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca
    55681 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    55741 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    55801 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    55861 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    55921 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    55981 gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc
    56041 tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag gtggtttttg
    56101 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    56161 cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa
    56221 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    56281 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg
    56341 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    56401 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    56461 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    56521 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    56581 ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg
    56641 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    56701 acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa
    56761 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    56821 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    56881 ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc
    56941 tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    57001 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    57061 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    57121 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    57181 tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc
    57241 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    57301 agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    57361 cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    57421 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt
    57481 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    57541 tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga
    57601 agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac aataagaaaa
    57661 tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt
    57721 cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac
    57781 catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa
    57841 agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc
    57901 tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta
    57961 aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac
    58021 ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc cgattttgac
    58081 ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt
    58141 gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg
    58201 acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc
    58261 ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag
    58321 tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg
    58381 gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg
    58441 cgcgaagctt aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc
    58501 tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa
    58561 cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc
    58621 gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat
    58681 tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc
    58741 agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga
    58801 ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    58861 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    58921 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    58981 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga
    59041 ccattaccgc tgagaaggga acaggcgcaa cgctaaacaa cgcgatgccc ccgccccaga
    59101 aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta
    59161 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    59221 cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc
    59281 ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca tctcttccag
    59341 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    59401 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    59461 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagac tggcaaaatt
    59521 gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    59581 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    59641 attgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt
    59701 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    59761 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    59821 cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    59881 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    59941 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc
    60001 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    60061 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    60121 tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca ggagactgaa
    60181 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    60241 tatgtccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    60301 tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    60361 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    60421 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    60481 tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga
    60541 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    60601 cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    60661 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    60721 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcgacaatca
    60781 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    60841 catcaaacgc atcatcgacc tcttctgcca ccgacggtgc gtggatcgtc aacgtaaagg
    60901 ttgaaccttt gccctgttca ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    60961 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    61021 tcacctggta atacatggcg aagattttat ccagctcatc ctgcggaatg ccgataccag
    61081 aatcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    61141 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    61201 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt aggctccagg ttaaagcgca
    61261 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    61321 aatcaaccgg ctgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    61381 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatagttttg agatattttt
    61441 cctgctcggc ggtgagttcg gtatccagca gaatgcggct caggccgacg ataccgttca
    61501 gcggtgtacg caattcgtga ctgatggtgg aaataaacgt cgttttgtcg cggctggcgc
    61561 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    61621 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc
    61681 gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt
    61741 tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca
    61801 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    61861 aaaactcttt atcttcgtta cgataaaaaa ccaagtcggg tgaagcatca aggaaggaac
    61921 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    61981 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcaattt
    62041 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    62101 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    62161 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    62221 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    62281 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    62341 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    62401 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    62461 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtagccacg gacgtcccag
    62521 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    62581 tcgattttcg caccacagcg gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    62641 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtataatc
    62701 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    62761 cacaatatgc agcggatgca gctttatgcc gtccacgctg gtttcaacca ctcgctccag
    62821 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac
    62881 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    62941 gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    63001 ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    63061 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    63121 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    63181 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    63241 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat
    63301 ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat gcaccttttg
    63361 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    63421 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    63481 gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca aaattaccga
    63541 aatttcatgc acaatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    63601 cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa
    63661 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    63721 aggaattgac tgtttctcta acgactctcc ttttagcctt aaagataaaa tctattttaa
    63781 tttcagctat ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    63841 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    63901 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    63961 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    64021 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    64081 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    64141 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    64201 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
    64261 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    64321 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    64381 ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt gtgggctggc
    64441 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca
    64501 ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc acgcgatatg gagcgtcgtc
    64561 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    64621 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
    64681 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catttgcctg ttccaccagc
    64741 gcttctccac taacaccgta ccgcgctggc cgctggcgca accgttccgc tatctggcgc
    64801 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
    64861 aattccagac gccgctgatc cctgacctgc acgacgccgc gccattcgtc aacgaaaccg
    64921 gttctgactc cagttccatg gataacatgc tggaactgct gctggcaggc gggatggata
    64981 tcatccgcgc catgcgcctg ttagtgccac ccgcctggca gaacaacccg gatatggacc
    65041 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggtccgg
    65101 cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
    65161 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
    65221 tctgggatta ccagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
    65281 tggtgatcga cactcgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    65341 aaagccgcca cccttataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    65401 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    65461 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
    65521 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
    65581 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
    65641 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
    65701 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
    65761 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
    65821 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctat
    65881 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgtt gctggtgctc tccgaccgga
    65941 atatcgctaa agatcgcctg ccggttccgg ctccgatggc ggttggcgcg atccagaccc
    66001 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    66061 gcgatccgca ccacttcgcc gtgttgctgg gtttcggcgc gacggctatt tatccgtacc
    66121 ttgcctatga aacgctgggc cgcctggtag acacccacgc gattgccaaa gattatcgta
    66181 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    66241 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctac
    66301 acgatgatgt agtgggcctg tgcttccagg gcgcggtcag ccgcattggt ggggcaagct
    66361 ttgaagactt ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc
    66421 cgattagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcctacaacc
    66481 cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc gactatcagg
    66541 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    66601 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    66661 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    66721 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
    66781 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
    66841 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    66901 agccgggcga aggtggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
    66961 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
    67021 tcgaagactt agcgcagctt attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    67081 ccgttaagct ggtgtccgaa ccaggcgttg gcaccatcgc gactggcgtg gcgaaagctt
    67141 atgcggactt gatcaccatc gcgggctatg acggcggcac cggcgcaagc ccgctgtcgt
    67201 cagtgaaata tgcaggctgc ccgtgggaac tcggtctggt ggaaacccaa caggcgctgg
    67261 ttgccaacgg cttgcgtcat aaaattcgtt tgcaggttga tggcggcctg aaaacgggtg
    67321 tcgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccaa
    67381 tggtggcgct cggctgtaaa tatctgcgta tttgccacct gaacaactgc gccacgggtg
    67441 tagcaactca ggatgacaaa ctgcgtaaga atcactatca cggcctgccg ttcaaggtga
    67501 ctaattactt tgagtttatc gcccgtgaaa cccgcgagct gatggcacag ctgggagtaa
    67561 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agagctggac ggtttcaccg
    67621 ccaaacagca gaaactggcg ctgtcgaagc tgctggagac tgccgagcca catgcaggta
    67681 agtcactcta ctgcactgaa aacaacccgc cgtttgataa cggcctactg aacgcgcagt
    67741 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
    67801 tccgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
    67861 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
    67921 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
    67981 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
    68041 tccgcagcca tgaagcaagc attatcggca acacctgcct gtatggcgcg accggtggtc
    68101 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca
    68161 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt attgtctgta
    68221 ttctgggtaa aaccggcgtt aacttcggcg cgggcatgac cggcggcttc gcttacgttc
    68281 tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgaa gtcttaagcg
    68341 tcgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag catgttcagc
    68401 ataccggttc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccacgaaat
    68461 tcgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcac cgtagtcgta
    68521 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
    68581 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgtaaaa
    68641 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc
    68701 gctgtctgtc gtgcggcaac ccatactgcg agtggaaatg cccggtacac aactacatcc
    68761 caaactggct gaagctcgcc aacgaggggc gtatttttga agcggcagaa ctgtcgcatc
    68821 agaccaacac actgccggaa gtatgcggac gtgtctgccc gcaagaccgt ctgtgcgaag
    68881 gttcctgcac cctgaacgat gagttcggtg cggtgaccat cggcaacatt gagcgttata
    68941 tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg aagcaaaccg
    69001 gtaaaaaagt ggcgattatc ggtgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga
    69061 cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc gggctgctga
    69121 ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct
    69181 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtacagctgg
    69241 acgatctgct gagtgattac gatgccgtgt tccttggcgt tgggacttat cagtcaatgc
    69301 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
    69361 ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg
    69421 aaggcaaacg cgtggtagta cttggcggtg gcgacactgc gatggactgc gtgcgcacgt
    69481 ctgtacgcca gggggcaaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
    69541 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
    69601 tccagccact ggggattgaa gtgaacggta acggcaaagt cagcggcgta aaaatggtac
    69661 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt
    69721 ccgaacatat cgttccggca gatgcggtga tcatggcgtt tggtttccgt ccacacaaca
    69781 tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgctccgg
    69841 aaggcaacga caacgctttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
    69901 tccgtggttc cgatctggtc gttaccgcca tcgccgaagg tcgcaaagcg gcagacggca
    69961 ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga aaagagtcgg
    70021 cgctgaaatt ctcctttctt tctggaaatc gcctcgcata atctgaagaa aaccaatgta
    70081 atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc cattattcaa
    70141 cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc tcaacaggct
    70201 accagcaaat ccacaacggc attattcatc tggtcgatag cgcccgaacg gaaacggtac
    70261 gtagcgttaa cgcgctgatg accgcgacgt actgggaaat tggccgacgg attgtcgaat
    70321 ttgaacaagg tggtgaggcc agagcagctt atggtgcaca gttgattgat cggctatcga
    70381 aggatttaag tcgacggtat aaacgtgggg tctctgcaaa aaacttacgc caaatgaggc
    70441 ttttttacc
//
LOCUS       DAJTNP010000007        67417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTNP010000007 DAJTNP010000000
VERSION     DAJTNP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..229
                     /locus_tag="OO160_000530"
     CDS             <1..229
                     /locus_tag="OO160_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5522772.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="OO160_000531"
                     /pseudo
     CDS             624..1263
                     /locus_tag="OO160_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="OO160_000532"
     CDS             1366..1689
                     /locus_tag="OO160_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5522773.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO160_000533"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO160_000533"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5522774.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO160_000534"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO160_000534"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5522775.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO160_000535"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO160_000535"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5522776.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO160_000536"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO160_000536"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5522777.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO160_000537"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO160_000537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5522778.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO160_000538"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO160_000538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5522779.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO160_000539"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO160_000539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5522780.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO160_000540"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO160_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5522781.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO160_000541"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO160_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5522782.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO160_000542"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO160_000542"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5522783.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO160_000543"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO160_000543"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5522784.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO160_000544"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO160_000544"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5522785.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO160_000545"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO160_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5522786.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO160_000546"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO160_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5522787.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="OO160_000547"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="OO160_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5522788.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="OO160_000548"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="OO160_000548"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5522789.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="OO160_000549"
     CDS             complement(21227..23059)
                     /locus_tag="OO160_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5522790.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO160_000550"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO160_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5522791.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="OO160_000551"
     CDS             complement(23807..24301)
                     /locus_tag="OO160_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5522792.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /locus_tag="OO160_000552"
     CDS             complement(24384..24839)
                     /locus_tag="OO160_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5522793.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="OO160_000553"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="OO160_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5522794.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="OO160_000554"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="OO160_000554"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5522795.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVARKQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="OO160_000555"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="OO160_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5522796.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO160_000556"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO160_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5522797.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO160_000557"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO160_000557"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5522798.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO160_000558"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO160_000558"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5522799.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="OO160_000559"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="OO160_000559"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5522800.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="OO160_000560"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="OO160_000560"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5522801.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="OO160_000561"
     CDS             33415..34308
                     /locus_tag="OO160_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5522802.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO160_000562"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO160_000562"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5522803.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO160_000563"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO160_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5522804.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="OO160_000564"
     CDS             complement(36224..36940)
                     /locus_tag="OO160_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5522805.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO160_000565"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO160_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5522806.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO160_000566"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO160_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5522807.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO160_000567"
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO160_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5522808.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO160_000568"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO160_000568"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5522809.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO160_000569"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO160_000569"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5522810.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO160_000570"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO160_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5522811.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO160_000571"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO160_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5522812.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO160_000572"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO160_000572"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5522813.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO160_000573"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO160_000573"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5522814.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="OO160_000574"
     CDS             complement(45789..46448)
                     /locus_tag="OO160_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5522815.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO160_000575"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO160_000575"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5522816.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="OO160_000576"
     CDS             complement(47343..48356)
                     /locus_tag="OO160_000576"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5522817.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO160_000577"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO160_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5522818.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="OO160_000578"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="OO160_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5522819.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="OO160_000579"
     CDS             complement(50649..51827)
                     /locus_tag="OO160_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5522820.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO160_000580"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO160_000580"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5522821.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="OO160_000581"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="OO160_000581"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5522822.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="OO160_000582"
     CDS             complement(55609..55887)
                     /locus_tag="OO160_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5522823.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO160_000583"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO160_000583"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5522824.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="OO160_000584"
     CDS             complement(56469..57278)
                     /locus_tag="OO160_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5522825.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO160_000585"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO160_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5522826.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO160_000586"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO160_000586"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5522827.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO160_000587"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO160_000587"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5522828.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="OO160_000588"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="OO160_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5522829.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="OO160_000589"
     CDS             complement(60979..61869)
                     /locus_tag="OO160_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5522830.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="OO160_000590"
     CDS             complement(61856..62380)
                     /locus_tag="OO160_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5522831.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="OO160_000591"
     CDS             complement(62377..62847)
                     /locus_tag="OO160_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5522832.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="OO160_000592"
     CDS             complement(62844..63392)
                     /locus_tag="OO160_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5522833.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="OO160_000593"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="OO160_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..64861)
                     /locus_tag="OO160_000594"
     CDS             complement(64139..64861)
                     /locus_tag="OO160_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5522834.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO160_000595"
                     /pseudo
     CDS             complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO160_000595"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66024..66779)
                     /locus_tag="OO160_000596"
     CDS             complement(66024..66779)
                     /locus_tag="OO160_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5522835.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66861..>67417)
                     /locus_tag="OO160_000597"
     CDS             complement(66861..>67417)
                     /locus_tag="OO160_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5522836.1"
                     /translation="FVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVP
                     DDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGN
                     YYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAW
                     LVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16829 a        18242 c        16743 g        15603 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaataa
      601 cgccttttat tgataattta catatgaaaa aattttcctt tttttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aagcctcatt atttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac ggtcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaggaa acatacaacg catgatacat atccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcaaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32521 aaccgttaag cctctttgag tctgctgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgagaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca atatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
    66121 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
    66181 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
    66241 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
    66301 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
    66361 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
    66421 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
    66481 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
    66541 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
    66601 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
    66661 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
    66721 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
    66781 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
    66841 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
    66901 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
    66961 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
    67021 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
    67081 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
    67141 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
    67201 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
    67261 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
    67321 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
    67381 atcaccattg ccgcagcaat agctgtttta acaaact
//
LOCUS       DAJTNP010000008        66973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTNP010000008 DAJTNP010000000
VERSION     DAJTNP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO160_000598"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO160_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5522837.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO160_000599"
     CDS             complement(1151..1237)
                     /locus_tag="OO160_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5522838.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO160_000600"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO160_000600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5522839.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO160_000601"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO160_000601"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5522840.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO160_000602"
     CDS             3586..3630
                     /locus_tag="OO160_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522841.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO160_000603"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO160_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5522842.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO160_000604"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO160_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5522843.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO160_000605"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO160_000605"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5522844.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO160_000606"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO160_000606"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5522845.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO160_000607"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO160_000607"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5522846.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO160_000608"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO160_000608"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5522847.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO160_000609"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO160_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5522848.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO160_000610"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO160_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5522849.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO160_000611"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO160_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5522850.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO160_000612"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO160_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5522851.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO160_000613"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO160_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5522852.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO160_000614"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO160_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5522853.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO160_000615"
     CDS             complement(16981..17745)
                     /locus_tag="OO160_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5522854.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO160_000616"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO160_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5522855.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO160_000617"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO160_000617"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5522856.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO160_000618"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO160_000618"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5522857.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO160_000619"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO160_000619"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5522858.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO160_000620"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO160_000620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5522859.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO160_000621"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO160_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5522860.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO160_000622"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO160_000622"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5522861.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO160_000623"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO160_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5522862.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO160_000624"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO160_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5522863.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO160_000625"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO160_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5522864.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO160_000626"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO160_000626"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5522865.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO160_000627"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO160_000627"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5522866.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO160_000628"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO160_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5522867.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO160_000629"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO160_000629"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5522868.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO160_000630"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO160_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5522869.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO160_000631"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO160_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5522870.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO160_000632"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO160_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5522871.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO160_000633"
     CDS             complement(40421..41815)
                     /locus_tag="OO160_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5522872.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO160_000634"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO160_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5522873.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO160_000635"
     CDS             complement(42158..43444)
                     /locus_tag="OO160_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5522874.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO160_000636"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO160_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO160_000637"
     CDS             complement(44451..45221)
                     /locus_tag="OO160_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5522875.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO160_000638"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO160_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5522876.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO160_000639"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO160_000639"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5522877.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO160_000640"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO160_000640"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5522878.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO160_000641"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO160_000641"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5522879.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO160_000642"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO160_000642"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5522880.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO160_000643"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO160_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5522881.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO160_000644"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO160_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5522882.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO160_000645"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO160_000645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5522883.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIIEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO160_000646"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO160_000646"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5522884.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO160_000647"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO160_000647"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5522885.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO160_000648"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO160_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5522886.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO160_000649"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO160_000649"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5522887.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO160_000650"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO160_000650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5522888.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO160_000651"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO160_000651"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5522889.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO160_000652"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO160_000652"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5522890.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO160_000653"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO160_000653"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5522891.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="OO160_000654"
     CDS             complement(66071..66289)
                     /locus_tag="OO160_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5522892.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="OO160_000655"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="OO160_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5522893.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16245 a        18236 c        17244 g        15248 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacgct tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgag gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccatga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac acatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctctatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg actaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt
    66901 gaggtattaa acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt
    66961 cataaatatc tcc
//
LOCUS       DAJTNP010000009        66528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTNP010000009 DAJTNP010000000
VERSION     DAJTNP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO160_000656"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO160_000656"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5522894.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO160_000657"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO160_000657"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5522895.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO160_000658"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO160_000658"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5522896.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO160_000659"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO160_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5522897.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO160_000660"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO160_000660"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5522898.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO160_000661"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO160_000661"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5522899.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO160_000662"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO160_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5522900.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO160_000663"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO160_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5522901.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO160_000664"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO160_000664"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5522902.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO160_000665"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO160_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5522903.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO160_000666"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO160_000666"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5522904.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO160_000667"
     CDS             complement(15665..16702)
                     /locus_tag="OO160_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5522905.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO160_000668"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO160_000668"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5522906.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19312)
                     /gene="ggt"
                     /locus_tag="OO160_000669"
     CDS             complement(17582..19312)
                     /gene="ggt"
                     /locus_tag="OO160_000669"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5522907.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSRIITTVLQMVVNSIDYGMNVAEATNAP
                     RFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDP
                     RSVDDLTAGY"
     gene            19432..19872
                     /locus_tag="OO160_000670"
     CDS             19432..19872
                     /locus_tag="OO160_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5522908.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19859..20602)
                     /gene="ugpQ"
                     /locus_tag="OO160_000671"
     CDS             complement(19859..20602)
                     /gene="ugpQ"
                     /locus_tag="OO160_000671"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5522909.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20599..21589)
                     /locus_tag="OO160_000672"
                     /pseudo
     CDS             complement(20599..21589)
                     /locus_tag="OO160_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21591..22436)
                     /gene="ugpE"
                     /locus_tag="OO160_000673"
     CDS             complement(21591..22436)
                     /gene="ugpE"
                     /locus_tag="OO160_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5522910.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22433..23320)
                     /gene="ugpA"
                     /locus_tag="OO160_000674"
     CDS             complement(22433..23320)
                     /gene="ugpA"
                     /locus_tag="OO160_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5522911.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23418..24734)
                     /gene="ugpB"
                     /locus_tag="OO160_000675"
     CDS             complement(23418..24734)
                     /gene="ugpB"
                     /locus_tag="OO160_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5522912.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25131..25844)
                     /gene="livF"
                     /locus_tag="OO160_000676"
     CDS             complement(25131..25844)
                     /gene="livF"
                     /locus_tag="OO160_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5522913.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25846..26613)
                     /gene="livG"
                     /locus_tag="OO160_000677"
     CDS             complement(25846..26613)
                     /gene="livG"
                     /locus_tag="OO160_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5522914.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26610..27887)
                     /gene="livM"
                     /locus_tag="OO160_000678"
     CDS             complement(26610..27887)
                     /gene="livM"
                     /locus_tag="OO160_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5522915.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27884..28810)
                     /gene="livH"
                     /locus_tag="OO160_000679"
     CDS             complement(27884..28810)
                     /gene="livH"
                     /locus_tag="OO160_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5522916.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28858..29967)
                     /gene="livK"
                     /locus_tag="OO160_000680"
     CDS             complement(28858..29967)
                     /gene="livK"
                     /locus_tag="OO160_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5522917.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30391..30774
                     /gene="panM"
                     /locus_tag="OO160_000681"
     CDS             30391..30774
                     /gene="panM"
                     /locus_tag="OO160_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5522918.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30973..32076)
                     /gene="livJ"
                     /locus_tag="OO160_000682"
     CDS             complement(30973..32076)
                     /gene="livJ"
                     /locus_tag="OO160_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5522919.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32347..33201)
                     /gene="rpoH"
                     /locus_tag="OO160_000683"
     CDS             complement(32347..33201)
                     /gene="rpoH"
                     /locus_tag="OO160_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5522920.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33446..34504)
                     /gene="ftsX"
                     /locus_tag="OO160_000684"
     CDS             complement(33446..34504)
                     /gene="ftsX"
                     /locus_tag="OO160_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5522921.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34497..35165)
                     /gene="ftsE"
                     /locus_tag="OO160_000685"
     CDS             complement(34497..35165)
                     /gene="ftsE"
                     /locus_tag="OO160_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5522922.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35168..36664)
                     /gene="ftsY"
                     /locus_tag="OO160_000686"
     CDS             complement(35168..36664)
                     /gene="ftsY"
                     /locus_tag="OO160_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5522923.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36814..37410
                     /gene="rsmD"
                     /locus_tag="OO160_000687"
     CDS             36814..37410
                     /gene="rsmD"
                     /locus_tag="OO160_000687"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5522924.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37400..37669
                     /locus_tag="OO160_000688"
     CDS             37400..37669
                     /locus_tag="OO160_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5522925.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37672..38031)
                     /locus_tag="OO160_000689"
     CDS             complement(37672..38031)
                     /locus_tag="OO160_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5522926.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38172..38798
                     /locus_tag="OO160_000690"
     CDS             38172..38798
                     /locus_tag="OO160_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5522927.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38872..41070
                     /gene="zntA"
                     /locus_tag="OO160_000691"
     CDS             38872..41070
                     /gene="zntA"
                     /locus_tag="OO160_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5522928.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41172..41417)
                     /gene="tusA"
                     /locus_tag="OO160_000692"
     CDS             complement(41172..41417)
                     /gene="tusA"
                     /locus_tag="OO160_000692"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5522929.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41638..42303
                     /locus_tag="OO160_000693"
     CDS             41638..42303
                     /locus_tag="OO160_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5522930.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42376..42933
                     /gene="dcrB"
                     /locus_tag="OO160_000694"
     CDS             42376..42933
                     /gene="dcrB"
                     /locus_tag="OO160_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5522931.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42937..44154)
                     /locus_tag="OO160_000695"
     CDS             complement(42937..44154)
                     /locus_tag="OO160_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5522932.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44288..45337
                     /locus_tag="OO160_000696"
     CDS             44288..45337
                     /locus_tag="OO160_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5522933.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45392..45979
                     /gene="acpT"
                     /locus_tag="OO160_000697"
     CDS             45392..45979
                     /gene="acpT"
                     /locus_tag="OO160_000697"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5522934.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46090..47664
                     /gene="nikA"
                     /locus_tag="OO160_000698"
     CDS             46090..47664
                     /gene="nikA"
                     /locus_tag="OO160_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5522935.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47664..48608
                     /gene="nikB"
                     /locus_tag="OO160_000699"
     CDS             47664..48608
                     /gene="nikB"
                     /locus_tag="OO160_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5522936.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48605..49438
                     /gene="nikC"
                     /locus_tag="OO160_000700"
     CDS             48605..49438
                     /gene="nikC"
                     /locus_tag="OO160_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5522937.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49438..50202
                     /gene="nikD"
                     /locus_tag="OO160_000701"
     CDS             49438..50202
                     /gene="nikD"
                     /locus_tag="OO160_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5522938.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50199..51005
                     /gene="nikE"
                     /locus_tag="OO160_000702"
     CDS             50199..51005
                     /gene="nikE"
                     /locus_tag="OO160_000702"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5522939.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51011..51412
                     /gene="nikR"
                     /locus_tag="OO160_000703"
     CDS             51011..51412
                     /gene="nikR"
                     /locus_tag="OO160_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5522940.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51532..51891)
                     /locus_tag="OO160_000704"
     CDS             complement(51532..51891)
                     /locus_tag="OO160_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5522941.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51888..52163)
                     /locus_tag="OO160_000705"
     CDS             complement(51888..52163)
                     /locus_tag="OO160_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5522942.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52222..53346)
                     /locus_tag="OO160_000706"
     CDS             complement(52222..53346)
                     /locus_tag="OO160_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5522943.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53346..56081)
                     /gene="rbbA"
                     /locus_tag="OO160_000707"
     CDS             complement(53346..56081)
                     /gene="rbbA"
                     /locus_tag="OO160_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5522944.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56078..57145)
                     /locus_tag="OO160_000708"
     CDS             complement(56078..57145)
                     /locus_tag="OO160_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5522945.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57511..59133)
                     /locus_tag="OO160_000709"
     CDS             complement(57511..59133)
                     /locus_tag="OO160_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5522946.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59395..60984)
                     /locus_tag="OO160_000710"
     CDS             complement(59395..60984)
                     /locus_tag="OO160_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5522947.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61369..62400
                     /locus_tag="OO160_000711"
                     /pseudo
     CDS             61369..62400
                     /locus_tag="OO160_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62487..62963)
                     /locus_tag="OO160_000712"
     CDS             complement(62487..62963)
                     /locus_tag="OO160_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522948.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62956..63720)
                     /locus_tag="OO160_000713"
     CDS             complement(62956..63720)
                     /locus_tag="OO160_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5522949.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63966..64727
                     /locus_tag="OO160_000714"
     CDS             63966..64727
                     /locus_tag="OO160_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5522950.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64834..65334)
                     /locus_tag="OO160_000715"
     CDS             complement(64834..65334)
                     /locus_tag="OO160_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522951.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65485..>66528)
                     /locus_tag="OO160_000716"
     CDS             complement(65485..>66528)
                     /locus_tag="OO160_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5522952.1"
                     /translation="GSTISLSQPLGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWR
                     GITFSINWAKNDLLTSHKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQ
                     VSLSGQNSGGVWWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQL
                     GANLSGGILITRDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQP
                     YRRDDVILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPI
                     PFGAVISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKK
                     SASGLYQIAATCL"
BASE COUNT        16167 a        17862 c        16327 g        16172 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccggtaa
    17881 ccagccaggt tttaccgtct ttcaccacaa tggtcggcga catcgacgac agcgggcgtt
    17941 tgttcggccc gacggcgttg gcatcaccgc ccaccagccc gtaaacgttc ggtacgcccg
    18001 gtttggcgga gaaatcatcc atctggttat taagcagaat accgctctca cccgcgacaa
    18061 tacccgtacc gaaggtggtg ttcagcgtat aggtcaccgc caccgcgtta ccgtctttat
    18121 ccactacaga gtaatgggta gtttgattac tctcataagg cgcaagcttg ccagggcgaa
    18181 tctcactgga cggcttcgct tttttgatat caatttgatc ggcaatggat ttggcatagg
    18241 ctttattggt cagtgcctgc cacgggactt tgacaaaatc cgggtcgcca agatattccg
    18301 agcggtcggc gtaggcgtat ttctccgctt ctgccatgat ttgcatcgca tcggcgctgc
    18361 caaagccgta tttcttcata tcgaagtttt ccagaatatt gaggatttgt acgatatgga
    18421 tcccgccgga ggatggcggt ggcatggagt aaacctgata cccgcgataa tcgccgctta
    18481 tcggagtgcg ttcgaccgct ttataggccg ctaaatcttc tttagtgatc aagccaccgt
    18541 ttttctgcat ctcctgggcg atctgttccg caatcgtgcc tttatagaat tcgtccgggc
    18601 cgttttcagc aatcatctcc aggctctttg ccaggttcgc ctgcaccagc gtgtcgccct
    18661 ttttcagcgg ctcgccctct ttccagaaga tagctttact gttttcgtga ttcggcaaca
    18721 cttcgctacc gtaggttttg agatcgtcag ccagcgcgtc gttaacgata aaaccatcgc
    18781 gtgccagttt aaacgcgggc tgcacgactt tgttcagcgg catggtgccg tatttatcca
    18841 gcgccagcga gaaacctgct accgtacccg gtgtgccgga agccagatgc gaagtgagtg
    18901 attttttgct gtccgggttg ccctgatcat cgaggaacat atcgcgggtc gctttggcgg
    18961 gtgccatttc gcggaaatcg atagccgtgg tattgccatt tttcgagcgg attaacataa
    19021 aaccaccacc gcccagattc cctgcctgcg gatgcgttac cgccagcgcg tagcccaccg
    19081 ccacggcggc atcaacggca ttcccgccct ccttgaggat atccaccccc acctgagtgg
    19141 cagtggcgtc cacagacgct accattccct gtttcgcgcg taccggatgg aagacatctt
    19201 cctccacacc atacgagacg ggcggcgcag gaggcgcggc ggcggcgcta aaacaacttc
    19261 ctgagagcag agcagcaatg gccacccggc gtaaaaacgt cggttttatc atcgttattc
    19321 tccagagatt aaggggcaac cccagctaag cctggtatat aactctgaat taatcatcgt
    19381 tttgtcggga agcgagtaaa cttaaaggat atcttcagag gaggatacgc gatgaaacga
    19441 cttctgattc ttacggcact cctgccgttt gtcggctttg cacagcccat taatactctg
    19501 aacaacccta accagccggg gtatcagata cccagccagc agcggatgca aacccagatg
    19561 cagactcagc aaatccagca aaaagggatg ctgaatcagc aactgaaaac gcaaactcag
    19621 ttgcaacagc agcatttaga aaaccagata aacaataatt ctcagcgggt gttgcagtca
    19681 cagccggggg agcgaaatcc cgcccggcag caaatgctgc ccaacaccaa cggcgggatg
    19741 ttaaacagca accgtaatcc ggatagttcg ttgaatcagc agcatatgct gccggagagg
    19801 agaaacggcg acatgctgaa tcagcccagc acgccgcagc ctgacattcc gttgaaaact
    19861 attgggccgt aaagttcgga ccaatcacgt caatcgcatc ggtacagatg caatccacgc
    19921 cccagcgcag caactctgcc gcgtgctggg gtttattgac ggtataaacc agaatccgta
    19981 atccggcgtc tttcaactgc atcactcgcg ctttatcgag taacttatga ttgagatgaa
    20041 tagagacgca gcccagccgc gcggtcagtt cgcgccagtc gtcgcgccac tcatcaagca
    20101 ataaaccacg cggcagttcc ggtgccgcct gttgtgcagc ttctaaagca tcaatctcaa
    20161 acgatgacag cagcggcggc gtcataccgg cccacagttg gcgtgccgcc agcgccacca
    20221 ttttgcccgt taatggtcca gtgccagtgg tgggtttgat ttcgatattc gccatcatcc
    20281 cgtgttcgcg acagcgttcc gccacctgcg aaagcaacgg cagcggctca cctttaaacg
    20341 ctttgctgta ccaactgccc gcatccacgc gcagtaaatc ctgccagttc agttcacccg
    20401 cgacgcccca gccgttgctg gtgcgttcga gattgtcgtc atggagcagg aagatctcgc
    20461 catctttcga taacttcgcg tcaaattcga tcatcttatg accgtatttt gccccgacgt
    20521 cgattgccgc cagggtgttt tccggggcca gcttaccgcc gccacgatga gcgacgatgc
    20581 ggggataagg ccagttactc atactcgttg tcctgtttca ccatcaaaaa gatgcagctg
    20641 attttccgcc agatgcagcc acagcgtgct gcctgccgtc gggcgctcct gatgcgccag
    20701 tcgcaccacc agcttctgct tgcgagctta gcgcaatatg ttccgggcga atgccgaggg
    20761 tcattttacg cccggcatac tgacggtagc caccgtttag cggtagcacg ataccgccgt
    20821 ccagttcgaa atgcgtgcct tcattattca tgcggcctgt cagcaggttc atcgccgggc
    20881 tgccgataaa actcgccaca aacaggctgg cgggcttttc gtagacttcc accggcgtgc
    20941 caatctgttc ggcaacgccg ccgttcatca ccattactcg ctgggcgagc gtcatcgcct
    21001 caacctgatc gtgagtaacg tagagtgaag tcgttttcag gcgacggtgc agctgttgca
    21061 gttcaagacg catctgtacg cgcagcttgg catcgaggtt agagagtggc tcatcaaaca
    21121 agaacaccgc cggatcgcgc acaatcgcgc gtcccatcgc cacacgctgg cgctgaccgc
    21181 cggaaagctc gcgcgggcga cgtttgagca gaccgtccag ctccagaatg cgcgccgctt
    21241 ctttaacgcg ctcggctatt tgctgcttgc ccatgccgcg aattttcagc ccccacgcca
    21301 tgttttcttc gacgctcata tgcggataaa gcgcgtaatt ctggaacacc atcgcaatcc
    21361 cgcgatcttt cggctccatt tcggtcacgc gctggtcgtt gatccagata tcgccttcgg
    21421 tcaccctctc cagcccggca accatacgca gcagcgtcga tttcccgcag ccagacggcc
    21481 cgaccatcac gataaattcg ccatccgcca catcaagggt cagcggttta atgacctagg
    21541 ttttgccatc ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca
    21601 ctatcgacca ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac tactggtggg
    21661 atcagggtta acaacatcgc cgccatcact gagttccatt ccgtggtgcc ttcgccagtg
    21721 gcgatcatcc ctttgatccc tgccacggtg gtgccgagat ccacgtcggt aataatcaac
    21781 aacggccaca aatactggtt ccagccgtaa ataaaggtga tcacaaacag cgccgccaga
    21841 ttagttttgg agagcggaaa aacgatgtcg caaaagaagc gcattggtga cgcaccgtcg
    21901 atccgcgccg cttccaccag ttcatccggc agcgtcataa agaactggcg aaacagaaac
    21961 gtggcggtcg ccgaggccat cagcggcagc gttaaaccgg cgtagctgtc gagcatcttc
    22021 aggttggcga tgacttccac cgtcgggaag atacgtactt caaccggcag catcagggtg
    22081 ataaaaatca tccagaagaa gaggttacgt agcggaaaac gaaaccagac aatggcaaat
    22141 gccgagagca tcgagacagt aattttgccg agcgtaatgc tgaacgccat cacaaagctg
    22201 ttaagcaaca tccgccagaa cggcgcgcta tttgtgccta ccccgttcac ccagatgttg
    22261 tggatgtttt ccagcagatg cgtgccgggg atgagcgtca tcggcgcggc gtagacggcc
    22321 tgtttatcca gcgtcgccgc gacaaacgcc acgtacagcg ggaagaggat caccgcgatc
    22381 ccgaggatca gcatggtatg gctgaatatc gtcagccacg gacggttctc aatcattggt
    22441 aacgcacctt gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca
    22501 tcagcaccat cgactgtgcg gcagacgaag ccagatccag tccggtaaaa ccttcgcggt
    22561 agatcttata aatcagcgtc gtggtggcct gaaccggccc gccggacgtg gcggcgtcga
    22621 tcaccgggaa ggtgtcgaag aaggcataca ccaggttcac taccagcagg aagaaactca
    22681 ccggggcgat aagcggcagc gcaatcttaa agaagcggcg aatcggccct gcaccgtcga
    22741 tggctgcggc ttcgatcaac gaacggggaa tggattgcag cgcggcatag aagaacagaa
    22801 agttgtagct gatttgcttc cacaccgagg caaacaccac cagaaacatc gcctgaccgc
    22861 tattttgcgc gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc
    22921 gaccggggtt aaacaggaag atccacaata cggcggcaac ggcgggagcc acggcatacg
    22981 gcagcaacat taaggtttgg tagaaacgac tgccgcgcac gatgtactcc accagcgccg
    23041 caaagaacag cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg
    23101 tccagaagga gtcgagataa tagctgtcat gaaacagcgt gacgaagtta tccagaccga
    23161 caaactggct ggagaaacca aacggatcga cgctttgtag cgagtaccac aacgcttcgc
    23221 ccgcaggcca gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcagataag
    23281 gcagccagcg cgagcggaac accggacggg atgatgacat tacggttaat tcctgaactg
    23341 tgccggatgc gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc
    23401 atccggcatt acactgatta agacttcgtc gatttctcaa agcggcgcag taactggttc
    23461 ccacgctcaa ctgcggtatc cagcgcctgc tgtggcgtct tcttaccggt ccacacgctc
    23521 tcaagctctt catccacaat cacgcggatc tgcggcatgt tgcccagacg aagccctttg
    23581 gtgaacggca acggcggctt gttcagcatc tgacgcgtcg cggtatccgc acctgggttt
    23641 ttctcgtaaa agccctgctc acgggtcagg tcatacgccg ctttggtaat tggcagataa
    23701 ccggttttct gatgccactc agcagcgttt tctggcttcg cgaggaagtc gagaaacttc
    23761 gccacgccgg tatacgtttc tttatctttg ccctgcatca cccacaggct ggctccgccg
    23821 ataatggcgt tttgtggcgc atctttcgca tcggcgtcgt aaggcatcat acctacgccg
    23881 tagttaaatt tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtggtcatc
    23941 gcgcaatcac cgttatagaa cttctcggtg gattcatcct tacgaccgac gtagctgaag
    24001 tcgcccttct tgttcatctc ctcgagcatg gcgatgtgtt tcacctgctc cggcttgttg
    24061 aactccagca ccgcgtccgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg
    24121 ttccaggcgc taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac
    24181 ttaataccgg aggctttcag ttttgcggaa tagtccgcca gatcctgcca ggttttcggc
    24241 ggctgttccg ggtctaaccc cgctttcttg aaggcgtctt tgttgtaata gagaacggaa
    24301 gtcgagctgt tgaacggctg ggagagtaag tggccggttt tgctgtcgga gtagtaaccg
    24361 gaaaccgtcg gcacaaactg cgactcatcg aactgaatcc ctgcctcttt aaacacgtca
    24421 tacaccggtt taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc
    24481 aaaatagccg gagcgttgcc ggtacgaaat acggcaatcc ccgcgcttaa attctgttcg
    24541 tagttgcctt tataggtcgg tacaattttg taatccggat tttcggcgtt aaaacgttgg
    24601 gccagagaat ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc
    24661 gtcactgcct gtgcattccc cattaacgcc agtccgagcg ccagtgctga agctgtataa
    24721 tgtaacggtt tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta
    24781 tgctcgcggg gtaacatgac atgctcgaat tacagaaaaa taactttttt gttacatttg
    24841 taagatagta aggtgtcaga aagatgacaa ggcggtgacg gcgtgggtga gggaaaatgg
    24901 gagatggggc acggataagc ggaaaaatag aaggtctgaa tcaaactcta cagattactc
    24961 atcgtttcat gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc
    25021 aatatattgc aactcaccca gtaggcctga taagcgcagc gcatcaggca attttgcatt
    25081 tgtcacctgt ctcaaaggag tcttttgact ccttatcaat caacgtgtta ttacccgcct
    25141 aaatacgcac ttctcactgc ttcgttcgcc agcagcgcgt cgccggtatc ggagagcacc
    25201 acatggccgt tttccagcac gtagccgcga tccgccagct ttagcgcctg gttggcgttc
    25261 tgctcgacga ggaagatagt catcccctgc tcgcgcagct gctcgatggt gtcgaaaatt
    25321 tgctggatga taatcggcgc aagaccgagc gatggctcat caagtaacaa caaacgcgga
    25381 ttgctcatca gcgcgcgacc aatcgccagc atctgctgtt caccgccgga catggtgccc
    25441 gcccgctgaa cacggcgctc atgcagacgt ggaaacagtt catacaccca ctttatgcgc
    25501 tcctgaaact ggtcacgttc ggcaaagaag ccgcccatcg ccaggttctc ttccaccgtc
    25561 atccgcgaga agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc
    25621 gccgtctgcc agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc
    25681 ggatcgccgc ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgaga
    25741 gtgacaatct cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg
    25801 tagtgggcgc tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa
    25861 ataggcacgg atcacgtccg ggttattacg gatttgctcc ggcgtaccgt ttgccagtgg
    25921 cgtcccctga ttgaccacat aaattcggtc tgaaatcccc atcaccagct tcatatcgtg
    25981 ttcaatcaac aaaatagtgg tgttgtgatg attgcgcagt tcggcaatca gctcatccag
    26041 ctctttggtt tcttttgggt taagacctgc cgccggttcg tcgagcatta aaatctccgg
    26101 ctgcgtcacc atgcagcggg caatctccag acggcgttgg tcaccatagg ccaggttact
    26161 cgcctgacgg ttggcgtgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc
    26221 gagcgcttcg ctctgggcgc gacggaaaga aggcgttttc aacaggccag agaacagccc
    26281 ggttttcagt tgctgatgct gcgccaccag caggttttca attaccgtca tttcacggaa
    26341 cagacgcaca tgctggaagg tgcgcaccac gcccatgcga gcaatttgct gccccggtaa
    26401 accttcaaga tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttgt agaaaccggt
    26461 caggcagtta aaaaccgtgg tttttccggc accgttaggg ccgattaacg agacgatttc
    26521 ctgcgggtac agttcaagat tgacgttgtt caccgccagc aggccgccga agcgcatcat
    26581 cagaccgtta acagataata atggctgact catgcctgct ctcctttcgc tgcgccgttt
    26641 ttcagcttca gttgcgggcg cgtcatgggc agcaaaccct gcggacgcca gatcatcatc
    26701 agcaccatca aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaattcg
    26761 cgcgacacca ccagcagaac agccgccaga atcactgcaa attgcgagcc cataccgccg
    26821 agcaccacta tcgccagcac aaaggccgat tcagcaaagg tgaaggattc cgggctgaca
    26881 aagccctgac gcgccgcaaa cagcgttccg gcaaaaccgg caaatgcggc gctgatggta
    26941 aaagcagtca gcttgatacg gcgcgggctt aagcccagcg aacgacaggc gatctcgtct
    27001 tcacgcaacg cttcccacgc gcggcccagc ggcatccgca gcaggcggtt aatgacaaac
    27061 aggcttaaca ccaccagcag caacgccacc aggtagagga agataacgcg atcggacgga
    27121 tcgtatttca ggccaaagaa attactgaac gtgtcccagc cgccttcacg agcggtacgg
    27181 ctgaactcga gtccgaagag tgtcggtttc gggatctgac tgattccgtt cgggccgccg
    27241 gtgatttcag tgttattgag cagcaatatg cgcacaattt cgccgaaacc aagggtaacg
    27301 atcgccagat agtcaccgcg cagacgcagc accggaaaac cgagcaggaa acccgccgcc
    27361 gctgccatca gtccggcaat cggcaaacag gtccagaacc ccagaccgta atagtgattg
    27421 agcagcgcaa aagtgtaagc gccgatggcg taaaaaccgc cgtaccccag caccagcaga
    27481 ccagaaagac caacaaccac attcagcccg agaccgagga taatgtagat catggtcaga
    27541 gtggcgatat ccaccgtccc acgtgacacc ataaacggcc acgccaccac aagcaccagc
    27601 agcgccacga ggaacagctt ttgcttcacc gtggacccat caatcgcggg cagaataaac
    27661 ttcggcccgg aaacgctttt caaccctttc tggaaagccg gtcgcaaaag ctggaagaaa
    27721 aagaccaccg ccgtgccgat aaacatccac tgccaacgga tgtctgaagc cgtgtcgacc
    27781 accagtttgg tgccatccag ctccagttgt acgcccataa agacgcccgc caggacaaag
    27841 aacatcgcgg cagagagcag tgccattgca atatgcatcg gtttcatact ttctctacct
    27901 ccgggcgacc cagaataccg gtcggcatca ccagcagcac cagaatcagc agggcgaatg
    27961 agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca
    28021 ggatcagccc gccaatcatc gcccctggaa tgctgccaat cccaccgagc accgcagcgg
    28081 taaaggcttt catcccggcc ataaagccga tataggggtt aatgacgcag tagaactgac
    28141 cgagcagtac gccagccact gccgccatcg ccgcgccaat aacaaaggtc aaagcaatca
    28201 cccggtcggt gttgatgcca agcaggctcg ccattttcag atcttccgcg caggcacgac
    28261 aggcgcgccc catacgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaagg
    28321 tgacaatcca gatcaccgcc tgcatggtgg taatagaggc agagaagttt tcgctatggc
    28381 ccaccaccca ctgaccgtta aacaggctcg gcagcgccac gtcgcgcgaa ccttcggtca
    28441 ggctgacgta gttttgcagg aagatggaca taccgattgc ggaaataagc gcaatcaggc
    28501 gcttagagtt acgcaccggg cggtaagcta cccgttcgat actccagccg taggcgctgg
    28561 cgatgacgat tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca
    28621 tcatcatcag cgcggcgatg atcataaatg agacgtagct gccaatcata taaacctcgc
    28681 cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca
    28741 gcgcgtaggt actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact
    28801 gctcagacat aaggtaacct ttctaaaccc gcccgcattt tacgggcggt gggatgatca
    28861 cttggctgcc gtggatgaac cgtcggcgtg ccactggaag acaccaaaat caaatccctt
    28921 aagatcgcct ttttcatccc agttcagcgg cccaatcacg gtgtttgcac cgttagcttt
    28981 taaatctttc accagcgcca gcggctcatc gctgccggta cgctcaaggg cagttgccag
    29041 agattgcact gccgcgtaag tgatccagac ataaggcccg gacggatctt tcttgtctgc
    29101 tttcagcgca tcaacgatgc cctggtttgc cggatcctgg tcatagcgtt ttggcatagt
    29161 gaccaacatg ccttcggcgg catcaccggc aatgttcgac agcgatgcgt taccgacacc
    29221 ttccggcccc ataaactggg ttttcaggcc aacggaacgg gcttggcgca gcatctgccc
    29281 catttccggg tagtaaccgc cgtagtaaac gaagtcgata ttttcttttt tcaggcgggc
    29341 gatcagcgcg gagaaatctt tctccccggc ggtaataccg tcgaagaaga cgacgttggc
    29401 gttagccgct ttcagcccgt cctgcaccga acgcgccagc ccttcgccat actgttgttt
    29461 gtcgtgaatg atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt
    29521 aggcccctgg gaagagtcca gcccggcagt acgcataatg tgttgataac cgcgttgggt
    29581 cagctccggg ttggtcgctc ccggcgagat catcagaata ccttcgtctt cgtagatatc
    29641 cgacgctggc tgggtagagg aagaacacag atgaccaata acgtatttaa tgccgtcgtt
    29701 aacgattttg ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac
    29761 gccaaccagc ttgtcgccct taattccccc tttgacatta atgtctttaa tcgcctgacg
    29821 cgcgccgtta aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac
    29881 ggcgacttta atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc
    29941 gatgatagtt ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc
    30001 tttgttttta tgtgttaaca aatcagactg ttcttttttt atactgcact gtttttgcct
    30061 gtctgattaa ggggttagcg cagtattttg tgatgatagc gattaaaatc cctatttttc
    30121 agtcgattaa gaacagataa tattctgaat ttattgatag ataaacagaa aaaagtgcct
    30181 ttgtcagcat aaaataacgg cacaaagggc ggaataattc actatcattc aggggattat
    30241 gctggacatt tttcattctc taatgtttta actttgtaat tattgctgtt aaaaaattaa
    30301 tcacctgcca aaagaaataa aaaagagaaa gcctccgatt aaattatttc gctacactgg
    30361 ttccactttt gtgatttaca cgggttaccc atgaagctga ccatcattcg attagaaaac
    30421 tttagcgacc aggaccggat tgacctgcaa aagatctggc cggagtattc cccttcctct
    30481 ttacaggttg acgataacca ccgtatctac gccgcgcgtt ttaacgagcg tctgcttgcg
    30541 gccgtgcggg taaccttaag cggcaccgag ggagcactgg attctctacg cgtgcgggaa
    30601 gtcacccgcc gtcgcggtgt ggggcaatat ctgctggaag aggttttgcg taacaatcct
    30661 ggcgtttcat gctggtggat ggcggatgca ggcgtggaag atcgcggtgt gatgacggcg
    30721 tttatgcagg cactgggatt tacggcacag cagggcggct gggagaagcg ttaatcgtca
    30781 agttgatttc aaaagtgatg accaacgaga aattgcctga tgcgctacgc ttatcaggcc
    30841 tataatgtgt gctgcaattt actgatttct ttggttcttg taggccggat aaggcgttta
    30901 cgccgcatcc ggcatgaagc aacgtattcg atattagcaa tttggcggca acccaaagtt
    30961 gccgattaac aattacttcg catcggtcgc cgtgccgttg gcgtgccagt caaatacgcc
    31021 gaactcaaag ccctttagat cgcctttctc atcccaggtc agtggcccca tcacggtatc
    31081 cacggagttc gcttttaggt atttggcgat ttcagccgga tcgtcagact gattcaggcc
    31141 cgcctgcaaa gattgcagcg cggcgtaggt ggtccagacg aatgcgccgc ttgggtcctg
    31201 ttttttcgct ttgatcgcat caacaatggg tttgttcgcc ggaacctgat cgtagttctt
    31261 cggcttggtc accagcagcc cttccgctga ttcgcccgca atgttagaca gcgaaacgtt
    31321 agccacacct tccggcccca taaactgagt tttcagccct gccgcgcgtg cctgacgcag
    31381 gatttgcccc atttccgggt gataaccgcc gtagtaaacg aagtcgatat tctctttttt
    31441 cagacgcgcc accagcgttg agaaatcttt ttccccggcg gtgataccat caaagaacac
    31501 cacgttagca ttgcctttct tcaggccgtc ctgcaccgct cgtgccagac cttcgccgta
    31561 ttgctgtttg tcgtgaacga tagcaatacg ctgcggtttc actttctcaa gaatatattt
    31621 cgccgccgtc ggcccctggt cggagtccag gccggtggtg cgcaggatca gctgatagcc
    31681 acgggcggtc agctccggcg cggttgccgc tggggtgatc attaaaatgc cttcgtcttc
    31741 gtagatgtca gacgcaggct gcgttgatga agaacagagg tgaccaatca catatttaat
    31801 gccgtcgtta acgactttgt tcgccaccgc aaccgcctgt ttcgggtcac aggcatcgtc
    31861 atattttacg atttgcagtt tgttgccttt aatgccgcct ttagcgttga tatccgcaac
    31921 cgcctgctct gcgccggtaa actcctggtc accgtactgc gcaaccggac cggacattgc
    31981 acccacgacc gcgactttaa tatcttctgc cagagccata ttgctgaatg ccagcgcgat
    32041 acatcctgcc agtaacgctt taccctttat gttcatcctg agaatcccca ttcttctggt
    32101 tattacgtgt gttgtgatgt tgttgttcag cactttattt cgttttatgc atgactaccc
    32161 gtgctttagc agcatactct gctaaaacat acccgatttt tatgatattg gaatagctat
    32221 tttgacagtt tattaacaat ctgcgtgggg attggcgttt tgccggaggg ggaattgatt
    32281 attgtagagg cccaaaaaac aaaaaccccg gactctcatc cagggttctc tgcttaatag
    32341 cggaaattac gcttcaatag cggcgcgaag ttttttcatc gcgttctttt ccagttggcg
    32401 cacacgttca gcggaaacac cgtaacggtc agccagttcc tgcaacgtgg acttgttgtc
    32461 ttcgtccagc cagcgcgcac ggatgatgtc ctggctgcgt tcgtccagac catgcatcgc
    32521 gtcggtcaga cggtttgccg cctgctcttc ccagttatca tcttcgatgc cgtcggcaaa
    32581 gttagatgat ttatcctgca gatagagcac cggtgccatc ggctggctgt cggaatcgtc
    32641 gtcggaagac aggtcaaagg tcatgtcctg tgccgccata cgtgattcca tctcacgtac
    32701 gtctttgctg gttacgccca gttcacgggc caccatttcg acttcatcct ggttaaacca
    32761 gcccagacgc tgcttggttt tacgcaggtt gaagaacagt ttgcgctgcg ctttggtggt
    32821 cgcaactttg acgatacgcc agttacgcag aacgtattcg tggatctctg ctttgatcca
    32881 gtggacggcg aaggagacca ggcgcacacc cacttccggg ttaaaacggc gcactgcttt
    32941 catcaggccg atgttacctt cctgaatcaa atccgcctgt ggcaggccat agcccgcata
    33001 attacgagca atatgaacaa caaaccgcag gtgagacagg atcagcgttt tagctgcttc
    33061 cagatcgcca tggtaatgca gcttttcagc cagcgcccgc tcctcgtcag ccgacaacat
    33121 cggccacgcg ttagctgccc ggatgtagga atccaggttg ccaactgggg ctaaagctaa
    33181 actttgcatt ttgtcagtca ttcaaatcct ctcaatcgat atcttctggc gcttcagtgg
    33241 tagcaacaac tgtgccaaag cttaagagca acgaggttat cattcactgt tttatcagac
    33301 cgtgatttta tccacaagtt caatgcaagc ttgtgaataa attacgcaca aaatgtgaca
    33361 tagagatgaa ataccgggaa gagacaacgg ggtctctttc cctgctacgg aacccattgc
    33421 agggaaagag tataacacgc ttttattatt caggcgtaaa gtggcgtaaa tgttgtaccg
    33481 tggcaagcca cgctgccacc cagccaatca tcgagcatac cagcagcaat agcaggcatt
    33541 catcgaacga taaaccattg atatcaaact tcgttccgaa aacctgtgcc acttccgcca
    33601 ccgctgacga caaacgcagt accagaattt ctgacaaaat taatgacaac aatgcgccag
    33661 aaaatcccag cagcgcaccg ccgtacagga acgggcgaag gatgaaacca tccgtcgcac
    33721 caatcagttt ctgcacgttg atggagtcac ggcgagcaaa gatgctcaga cgtacgctgt
    33781 tgccgatgac gaggaacact gccgccacca tcaacacgcc gatcatcgcc gaaacgcgcc
    33841 caaccagccc ggtcaacgcc gccagacggg caaaccagct gtcatccatc cgcacttcgt
    33901 caataccgtt aatctgcgtg atacgatcac gcagcgtatt gagtgattcc gtcccctgaa
    33961 aatcgagttt cgggatcacc accgccactg ccggaagcgg gttttcttcc agcatatcca
    34021 gcgcaccacc aaaaccagac cagttacgga actcgcccag tgcgtcttca cgagaaagat
    34081 agttcacttt ctccacgcct tgctcggcct gcaactgtgc caccacgccc gcagcggcgt
    34141 catcgtccag cgttttttgc aagtaaacag tgatttgcgg tgacggataa tactgcgtcg
    34201 ccgcctgatt aacgtttttg tacaccatat aacagacgct gggcagcgtc agggagatgg
    34261 cgataaccat caccgttaaa aacgtggcga acggtttgct tttcagatcc tgcaatgcgc
    34321 cgtggaaggc atagcgcacc tgttcgttga aaacgttggt tttgcgattt accggttttg
    34381 gcgaggattt cgcgcgtttc ggtgcgttac gaccgccgtc gcctgagccg ccgaccgatt
    34441 tacggaagcg atccagacgc ccaccaaact gccgaatatg attgattgca tcgcgcttat
    34501 tcatggccca cgcctccatg caagtgacca tcgctcaggg tgagcatgcg ataggaacgc
    34561 cgtgaaatca gattaatgtc gtgcgttgcc atcaatacgg ttaccccaac gcggttaaac
    34621 tcttcaaaca gacgtagaat cccttccgac aacgcgtcgt ccaggttacc ggtcggttcg
    34681 tccgccagca gtaccgcggg cttattcacc accgcgcggg caatgccaac acgctgttgt
    34741 tcaccgccgg aaagctgaat agggaagttc ttcgctttgt ccagtagccc gactttatcc
    34801 agtgccgccg acacccggcg acgaatatcg tcaccgctgg caccagcgat aatcagcggg
    34861 atcgccacgt tatcgtagac agtacggtcc atcagtagat ggtgatcctg gaaaatcatg
    34921 ccaatctggc ggcgcagaaa cggaacttca cggtttttca gacgcgtgat gtcatggccg
    34981 ctaaaccaga ttttcccggc gctgggccgc tcaatcccac agatcagctt caggagggta
    35041 cttttccctg cgccggaatg accggtcaga aacgccatct cacccggctg catatggaac
    35101 gtaacgccct gcagcgcctg tctcccaccg agataagcct tgctgacatg ttcaaagcga
    35161 atcattgtta atcctctcgg gcaaaaagtg cctctataaa gtcgtccgcc ttaaacggac
    35221 gcaaatcctc aatacgttcg ccgacaccaa tgtagcggat agggatacca aactggtcag
    35281 ccaccgagaa aattaccccg cctttcgccg tgccgtccag tttcgttagc gtgatgccgg
    35341 ttaagccaac agcttcgtgg aacagtttgg cctggcttac cgcgttctgc ccggtgctgg
    35401 catcaatagt cagcataact tcatgcggcg cttcaacgtc gagtttcttc atcacgcgga
    35461 cgattttctt caactcttcc atcagatgcg atttgttctg cagacgtccg gcggtatcgg
    35521 caatcaggac gtcgatatta cgcgctttag ctgcctgaat ggcgtcgaag ataacagagg
    35581 cggaatccgc accggtatgc tgggcaatca ccggaatatt gttgcgctga ccccagacct
    35641 gaagctgttc aaccgcagct gcacggaaag tatcgcccgc cgccagcatc accgatttac
    35701 cctgctgctc aaactgacgc gccagcttac caatcgtcgt ggttttaccc acaccgttga
    35761 cgcccaccat caggatcacg aacggcgttt tatcttcaac attcagcggc tcatcgactt
    35821 tcgccagaat ctcgcccatc tcttctttca gcaggccata gagcgcctcg gcatcacgaa
    35881 gctgcttgcg ggatgcgcct tccgtcagat tggtgataat tttacgtgtg gtttccacgc
    35941 ccacatcggc gatcaacagc tgttcttcca gctcttcaaa cagatcatcg tcgatttttt
    36001 taccgcggaa caggctgata aatccggaac cgagattttc tttggttttt aacaggctgc
    36061 gtttcaggcg cgcgaagaag ccttctttgg tcggtttttc ttgctcctgg gcaatttctg
    36121 caaccggctg ctcttcttct gccggaggaa ctaccatcac cgcctcttct gccgcttcgg
    36181 cagccagcgc ctgtgcttcc agctcttcgt cggtgatttc ttctttagcc gcttcttctt
    36241 ctgccgcttc gacaatctct acggtttccg cttcagcctg ccactcttct ggcgaaacct
    36301 cttcggcgtt gacgtcttcc ggcaacggca gctcttcacg ttcgatagcc actggctccg
    36361 gcgtttcttc tacgaccggt tccggctgtg caacaacttc cgcttcaggc tgcgcttttt
    36421 cactttcaac aacctgttca gtgacttcca caacgtcggc agcaaaagtt tccgcctcgg
    36481 cttcagtatg cgcctgcggc tgctcttcaa cggcttgttc agaagccttc gcaggctctt
    36541 gcgcctgaac gatttcttct acaaccggtt gttcattctg aacttctgtc tctttttccg
    36601 gggtctgctc tttttgacca aagcccagcc aggaaaaaaa gccacgtctt ttttctttcg
    36661 ccatttgcga ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata
    36721 gcagctaaaa aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa
    36781 gtatgtgtta tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc
    36841 ggccaaatcc gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagc
    36901 ccaggtctgc gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg
    36961 gtcattgttg acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa
    37021 gcgttatcgc gctacgctgc gggggcaacg ttgattgaga tggatcgcgt ggtttctcag
    37081 cagttaatta agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac
    37141 gcaatgtcat tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg
    37201 ttccgccgtg gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct
    37261 gacgaagccc tgatttatgt cgaaagcgaa gtcgaaaacg gtctgcccac tgttccggca
    37321 aactggtcat tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacac
    37381 gaagcacaag gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgcgtttg
    37441 gggattttta atcctcaatc tggtgcatcc cttcccgcgc ccgctaaata ttttcgttaa
    37501 cgtggcgctg atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac
    37561 tttaccgaaa gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg
    37621 cgtgtttgaa ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc
    37681 gccaacaaag ctaacaaagc gcgttccttt atagctcagc gtacctttat cgcccactgt
    37741 cagggcgtgg tactgctggg cgtcgaggcg aaacgtctgc tccattcctc cgctttgcgg
    37801 tttgaagctt gcctcatagc gcatactggt gcctgccgga gtcacttcct gctggcgcga
    37861 acggcgatcg ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag
    37921 cggagccata tcattatcag ctttttcccg tcgctgttgc ataaaacgaa acgatgcggc
    37981 gacgacaatt aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc
    38041 tcatcggaaa atgcggaatt aagcataccc tgccagttat ggtgttgtca tccgtccacc
    38101 ctcgccacta aactggaagc aagaccgtag gcattctgct tacgaaaaaa taacgaattc
    38161 aaggaactaa gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg
    38221 atgcatcgta tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc
    38281 tcctgctgtt actggcctgg caagcaccga tgttcgacgc cattagctat ctggtgctgg
    38341 cgggactgtg cgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga
    38401 tgtacgccat cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc
    38461 agatgacgct ctctttcttc tggcctctgc cactggtgtt gctggttctg ggtgcgctgc
    38521 tactggcgat tatctggacg cggctggaag agtaccgttg gcctatctgc acgtttatcg
    38581 gcatgacgct ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc
    38641 cggcgctctc tgcgtttgtt ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg
    38701 ggagccacta tcgccgacgc ttccgtgcgg ataacgcgat tgctgcggcc tgctactttg
    38761 ccggtcactt cctcatcgtc cgctcactgt atctctgata aaacttgact ctggagtcga
    38821 ctccagagtg tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgaca
    38881 cctgacaatc acggcaagaa agcccctcaa tttgctgcgt tcaaaccgct aaccacggta
    38941 cagaacgcca acgactgttg ctgcgacggc gcatgctcca gctcgccaac gctctctgaa
    39001 aacgtctccg gcacccgcta tagctggaaa gtcagcggca tggactgcgc cgcctgtgcg
    39061 cgcaaggtag aaaatgccgt gcgccagctt gcaggcgtga atcaggtgca ggtgttgttc
    39121 gccaccgaaa aactggtggt tgatgccgac aatgacatcc gtgcccaggt tgaatctgcg
    39181 gtgcaaaaag cgggctattc cctgcgcgat gagcaggccg ccgacgagcc acaagcatcg
    39241 cgcctgaaag agaatctgcc gctgattacg cttatcgtga tgatggcaat cagctggggt
    39301 ctggagcagt tcaatcatcc gttcgggcaa ctggcgttta tcgcgaccac gctagttggg
    39361 ctgtacccga ttgctcgtca ggcattacgg ctgatcaaat ccggcagcta cttcgccatt
    39421 gaaaccttaa tgagcgtagc cgctattggt gcactgttta ttggcgcaac ggctgaagct
    39481 gcgatggtgt tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagccgc
    39541 gcgcgtcagg gcgttagcgc gttaatggcg ctgaaaccag aaaccgccac gcgcctgcgt
    39601 aacggtgagc gggaagaggt ggcgattaac agcctgcggc ctggcgatgt gattgaagtc
    39661 gccgcaggtg ggcgtttgcc tgccgacggt aaactgctct caccgtttgc cagttttgat
    39721 gaaagcgccc taaccggaga atccattccg gtggagcgcg cgacgggcga taaagttcct
    39781 gcaggagcca ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc
    39841 agcgccattg accggattct gaaactgatc gaagaagccg aagagcgccg cgcccccatt
    39901 gagcggttta tcgaccgttt cagccgtatt tatacgccag cgattatggc cgtcgccctg
    39961 ctggtaacgc tagtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa
    40021 ggtctgacgc tgctgctgat tggttgcccg tgtgcgttag ttatctcaac gcctgcggcg
    40081 ataacctccg ggctggcagc agcagcgcgt cgtggggcgt taattaaagg cggtgcggcg
    40141 ctggaacaac taggtcgtgt tacccaagtg gcgtttgata aaaccggtac gctgaccgtc
    40201 ggtaaaccgc gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg
    40261 acactggcgg cggcggtcga gcaaggcgcg acgcatccac tggcgcaagc catcgtacgc
    40321 gaagcacagg ttgctgaact cgccattccc accgccgaat cacagcgggc actggtcggg
    40381 tctggcattg aagcgcaggt taatggtgag cgcgtgttga tatgcgctgc cgggaaacat
    40441 cccgctgatg catttgctgg tttgattaat gaactggaaa gcgccgggca aacggttgtg
    40501 ctggtagtac gtaatgatga cgtgctgggt atcattgcat tgcaggatac cctgcgcgcc
    40561 gacgctacaa ctgccatcag tgaactgaac gcgctggggg ttaaaggagt gatcctcacc
    40621 ggcgataatc cacgcgcagc ggcggcaatt gccggggagc tggggctgga gtttaaagcg
    40681 ggcctattgc cggaagataa agtcaaagcg gtgaccaagc tgaatcaaca tgcgcccctg
    40741 gcgatggtcg gtgatggtat taacgacgcg ccagcgatga aagctgccgc catcgggatt
    40801 gctatgggta gcggcacaga cgtggcgctg gaaaccgccg acgcagcatt aacccataac
    40861 cacctgcgcg gcctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc
    40921 cagaacatca ccattgcgct gggactgaaa gggatcttcc tcgtcaccac gcttttaggg
    40981 atgaccgggt tgtggctggc agtgctggca gatacgggtg cgacggtgct ggtgacggcg
    41041 aatgcgttaa ggttgttgcg caggagataa ggcaaaccga tcgcaacatt gagcgcgatc
    41101 ggtcccctcg cccctctggg gagagggtta gggggtgagg aaaaggcggc atcgaagcca
    41161 atcagcccct atcaaccgcc tttacgaatc aaataacgat aaggcagtcc atccgtctct
    41221 ttcgcaacca gttcgtgttc cataaaggta caaaacccag gaatatcgcg ggtagtggcc
    41281 ggatcgtcgg cgataatcag caacgtttcg ccaggctgca tattgcgcac ggttttgcgc
    41341 accatcatca ccggttccgg gcagcgcagg ccaagcgcgt cgagtgtgtg gtcagggctg
    41401 gaaaagagat cggtcatttt cttctcatca cttaaaaaaa cggcgctagt ttacgccctg
    41461 tgagtccgta agcaaaccag gttaacgatt gcgtgaaaat taaccattgc attgtcaacg
    41521 taaagcagta tcatgcggcg gctcgaaaaa agggtaagca cgttattatg ttaaggtaac
    41581 agacgtgtcg tacgtattgg gttccctcac cccaatggtt aatcaaaaag gtacaatatg
    41641 aacgttttct cgcaaactca acgctataag gcgttgttct ggttatcgtt atttcatctt
    41701 ctggtgatca cctccagtaa ctatctggtt cagcttcccg tctccatttt tggtttccat
    41761 accacctggg gcgcgtttag ctttccgttt atttttcttg ctaccgacct gaccgtgcgt
    41821 atttttggcg caccgctggc ccgacgcatt atcttcgcgg taatgatccc tgcgttatta
    41881 atctcctacg tcatctcgtc gctattctat atgggttcct ggcagggatt cggcgcactc
    41941 gcccacttca acctgtttgt cgcccgtatc gccaccgcca gtttcatggc ctacgcgctg
    42001 gggcaaatcc tcgacgtgca cgtttttaac cgcctgcgtc agagtcgccg ctggtggctg
    42061 gcaccgacag cgtccacact gttcggtaac gtcagcgaca cgctggcctt tttcttcatt
    42121 gccttctggc gtagcccgga tgcctttatg gctgaacact ggatggaaat cgcgctggtc
    42181 gattactgtt tcaaagtgtt aatcagtatc gttttcttcc tgccaatgta tggcgtatta
    42241 ctcaatatgc tgttgaaaag actggcagat aaatccgaaa tcaacgcttt gcaggcgagt
    42301 taaaggttcg ttatcagagt tgtgataaga tggatgaatg agccgttatg gccgtttatc
    42361 gaaaggaaga agtcaatgcg caatctggtt aaatatgtcg gaattggcct gctggttatg
    42421 gggcttgcgg cctgtgatga taaagacact aacgctacgg cgcagggttc ggttgcggaa
    42481 agtaacgcta ccgggaatcc cgtcaacctg cttgatggca agttaagttt ctcgctgcca
    42541 gcggatatga ccgaccagag cggtaagctg ggaacgcagg ccaataacat gcatgtctgg
    42601 tccgacgcca ccgggcagaa agcagtcatc gtcatcatgg gcgatgatcc gaaagaagat
    42661 ctggcggtgt tggcgaagcg tctggaagat cagcaacgta gccgcgatcc gcagctgcaa
    42721 gtggtaacca ataaagccat tgagctgaaa ggtcacaaaa tgcagcagtt agacagtatt
    42781 atctccgcga aaggccagac ggcgtactct tccgttattc tgggtaacgt gggtaatcaa
    42841 ctgctgacca tgcaaattac gctgccagct gacgatcagc aaaaagcgca aaccaccgca
    42901 gaaaacatca ttaatacgct ggttattcag taagttttaa gatgatgagg cggcctcagg
    42961 gacgtgttcc ggaggccgtt tttttaatcg ccacgtcagt aataacgcga ttgcgaccag
    43021 tcccgccgcc gccagataaa tcaccggtac gcccgcccag ctcatcacca gcccagccag
    43081 tggtccagtc acgccaagcg ataaatccat aaatacggtg taagttgcca gcgccgcccc
    43141 ctgattttgc tgcggaaccg cttttaccgc cactacaccc aatgccggga acaccagcga
    43201 aaacccggcc cccgccagta agacgccgat tttcgccatc cacggcatag tcgccacgcc
    43261 aaccagtagc aggccgatta tctcaacgct aaagcaaatc atcgctacgt ttaagccacc
    43321 gatacggtta atgccgttag ggaataacaa acgcgtaccg acaaacgcac agctaaacag
    43381 cgtcagcgcg aaagccgcac cgtcccaacc tttagcgtca taaaacagcg tgataaaggt
    43441 ggcgatgacg ccaaatccgg cggaagccag tgccagcgcc ataccgtaca gccagacgcg
    43501 cccaagcacc gcgcgaaacg gcagcggttt gcctttactg gcttttaccg tcggacgcgg
    43561 gatcgccaac aaaatggcca ccagcgccac gcccataatg attaacgcta acgcctgcaa
    43621 gccgccccag tgataaaaca cgacgcctaa cggcgcaccc atcgccatcg ccccgtaagt
    43681 gacaatgccg ttccacgaaa tcacccgccc gatatgcagc gagccaacca ctccaacgcc
    43741 ccatagggtc gatcccgttc cggcaaaact ttgcccaatc ccaaggatga cgcgccccag
    43801 gcaaagtaat aacaggctga tgacaggcag actggcggtt aatcctgccg tcagataccc
    43861 cagaccgctc aaaaagcagc cgcataaacc gaagacgaca atctttttgg gtcccagcaa
    43921 atcggcgtaa cgtccggcat gagggcggct cagcaaggtg gcgaaatatt gcaggctgat
    43981 aaccaatcct gcccagaagg cgctaaagcc catcacatca tggacatagc ccggtaatac
    44041 agcgagcggc aacccgatgg tgaggtagct ggcgaagtta aacatgacaa tggagacaat
    44101 gcgcaaattc aggcgcaatc cgtttagcgc gggttcggct acgggttcgg gcatgaggat
    44161 caccacattt ttacaacagt gtttcatttt taccatgtgc tgacgtgaaa atcagcagta
    44221 agaatcagaa tattgctggc gtgactcccg cgctacactt aacacaaaaa gtcacaagga
    44281 agccccaatg gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc
    44341 ctcgctggta gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt
    44401 actggcgctg ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg
    44461 agtacaacgc cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc
    44521 gctggtcggc gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa
    44581 taaggagctg acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca
    44641 tatgtcgccg gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac
    44701 agcgctaatg accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt
    44761 agtttttatg ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa
    44821 cccacagatt cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc
    44881 gttaaagacg ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat
    44941 gggcgtacag tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa
    45001 tatcggcgcg gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt
    45061 ttacgaatgt attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat
    45121 tttagaacca cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc
    45181 attgttaatc tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac
    45241 cagcgtgtgt aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact
    45301 ggggccgggc agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca
    45361 ctttcatttt ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc
    45421 gaccttaagc gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg
    45481 acgcgaacgc tggctggcgg ggcgtacatt gctttcgcac acgctttccc cgctaccgga
    45541 gatcatctat ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa
    45601 cttaagccat agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg
    45661 cgatatcgaa gtgattcgcc cgcgcgtcaa ctggcgctgg ctggcgaacg ccgtgttcag
    45721 cctcggggaa cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg
    45781 gcgcatctgg acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat
    45841 cgtcagcgta gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa
    45901 tttaagcctt gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg
    45961 gatcgattca gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta
    46021 atcagtatga cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt
    46081 ggggttacca tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct
    46141 tttatcgtcc atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg
    46201 ccactaaacc cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa
    46261 ccattggtga aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact
    46321 cattcagaag atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac
    46381 ggtgaaccgt tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt
    46441 caacgtcacg cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa
    46501 acagagctgc aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg
    46561 ccccgtcctt tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga
    46621 attaaagcgc cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac
    46681 gatgtcttcg tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc
    46741 tttaacgtca tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac
    46801 ctgctgtacg gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat
    46861 ccggcttacc acacccaact gtcacagccg atcgaaaccg tgatgctggc gctcaatacc
    46921 gccaaagccc ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa
    46981 aaatcgctga ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc
    47041 ccttctgtgc cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg
    47101 aaagagttgc tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa
    47161 aatggtcagc cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg
    47221 atggcggaaa tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc
    47281 gaagaagaga gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac
    47341 cgcacctggg gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg
    47401 cacgctgact tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc
    47461 ggcgaagtgc tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg
    47521 acccgtctgc atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta
    47581 tcaaaaccgg agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa
    47641 cagattaaac cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga
    47701 tcccgatggt gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg
    47761 acccggcgct cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct
    47821 ctacccgcac catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt
    47881 ggaaggcgct gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg
    47941 atatgctgaa cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc
    48001 tgctcacttc cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt
    48061 tcgccgtgcg ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt
    48121 tgctagtgat ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct
    48181 ggcagcacat cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc
    48241 gactgctgcg cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc
    48301 tacgcggcct gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc
    48361 cgatgatcac cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg
    48421 aaaacatctt tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg
    48481 actatccggt gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt
    48541 tgattgtcga tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc
    48601 acgcgtgaac tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc
    48661 cctgctggcg ctgattgcgc tcaccagcca gtggtggcag ccgtacgatc cacaggcaat
    48721 tgatttgccg tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt
    48781 aggtcgcgat attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat
    48841 ggcctgcctg ctgctggtgc tgacattagg gctggttatt ggcggcagcg ccgggttgat
    48901 tggcgggcac gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac
    48961 ctcgattctg tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat
    49021 cgccatcgcc ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc
    49081 actacgccaa cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt
    49141 gtttgtcgat catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga
    49201 tatcggccat atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc
    49261 gccgaccgcc gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc
    49321 gctgcaaatg ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt
    49381 gggtgacgca ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg
    49441 ccgcaacaga ttgaactacg taatatcacg ctacaggccg cgcagccgct ggtacacggt
    49501 gtatcgttaa ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg
    49561 aaatcattaa cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg
    49621 ggggaaattt tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt
    49681 gccaccatca tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac
    49741 gcgcgggaaa cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc
    49801 atagaagcgg tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg
    49861 agcggcggca tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt
    49921 atcatcgccg atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat
    49981 ctgctggaaa gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg
    50041 ggcgttgtgg cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa
    50101 cagggcgatg tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt
    50161 tccgctcatc tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg
    50221 gcctttccca tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc
    50281 tgaataacgt ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg
    50341 gctgcggaaa aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga
    50401 atattagctg gcacggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc
    50461 gccgtgatat tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg
    50521 tgcgcgagat cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac
    50581 tggcgcgcgc cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac
    50641 gcccaccgca gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg
    50701 tcgaaccgaa attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg
    50761 cgggcgtcat tcgcctgctg aaaaagctac aacaacagtt tggcactgcc tgcctgttca
    50821 tcactcatga cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg
    50881 gacaaatcgc cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac
    50941 gtgtgctaca aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg
    51001 tttaacgcca atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga
    51061 cagcctgagc cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg
    51121 tagtgccctg gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc
    51181 gtatgtgtat gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca
    51241 tcaccacgac ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga
    51301 aatcgccgtg ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc
    51361 ccagcgcggc gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc
    51421 taaaacaagg aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca
    51481 tagatagatc ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg
    51541 attcaaacgc tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc
    51601 agccgcatta ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat
    51661 cttttctgta cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg
    51721 cagggaaagc tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata
    51781 acctgacaag cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac
    51841 agctggttgc ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc
    51901 cctatactta gtaaccagtc acacacgctt tctacagcgc ctttatcggt atctggcgac
    51961 ggatgaggcc gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct
    52021 cttccttcct taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg
    52081 atcccttgag gaacaggagt tttaaatatt tgatccaatg tatttttttg tttcttccgt
    52141 aaagcctgta ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt
    52201 atgaagtcaa gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag
    52261 tgcgatggtg aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat
    52321 ttcgaatccg gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg
    52381 catggtcagc ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc
    52441 ggaaagcatt tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga
    52501 acgcgctatc gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac
    52561 gcccagcata aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat
    52621 cagcaccagc gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc
    52681 catcatgatc tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc
    52741 acgctcgcgg atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat
    52801 gatcgccatc accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt
    52861 ttccagcgat accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc
    52921 accgttgata atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc
    52981 atcgacgttc acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg
    53041 cggaatattt atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc
    53101 ggtgatcatc tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga
    53161 taactgcgat tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt
    53221 aacggtcgct gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc
    53281 tttatcaccg agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaaatg
    53341 gcgcatcatc cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg
    53401 gctatcagca acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac
    53461 gttccgcggg cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca
    53521 ggcccttcca gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat
    53581 gtgataatcg ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc
    53641 agccccattc cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa
    53701 ctgcctttat gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc
    53761 agcatcccca gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg
    53821 ggggtcacgt aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg
    53881 cttagcattg acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc
    53941 aggcttttta cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg
    54001 ctggcgggtg tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc
    54061 acgtaacctt ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt
    54121 tccacaggcg taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc
    54181 gtgatatcgc ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt
    54241 tcgataaagt aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg
    54301 tcgcggtcga gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt
    54361 atcagcatca ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc
    54421 agttccagcg cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc
    54481 ggcgcgtggg tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc
    54541 gcttcctgca aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc
    54601 agttcctgcg gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg
    54661 caacgttccg cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc
    54721 gagagatcga ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta
    54781 ggctcatcga ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc
    54841 tggcgaatgc cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa
    54901 cgctcgctca tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg
    54961 gcatgtaact ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac
    55021 atatagccca cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc
    55081 cacgcctcac cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta
    55141 ccgcagccgt tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg
    55201 tgatcaacgg caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc
    55261 tctgcgtttt caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct
    55321 tgcggtaaca gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc
    55381 tcttcggcgc tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag
    55441 cgttcggcct cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg
    55501 cgaatactgt cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt
    55561 ggctcatcaa ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt
    55621 tgcttcatcc cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg
    55681 ctggtcagca gctcattaat tcgcgcttcc cgctccgctt tatcgtgacc gaacaggcga
    55741 gcgaaaaaat cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc
    55801 tgcggcatcc aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg
    55861 ccacccagca ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat
    55921 aaactcgact tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata
    55981 tcgagagtga tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt
    56041 tgcgcgacag gcgggacggg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga
    56101 ggtcgtcagg ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg
    56161 ttttgacata ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga
    56221 acatcagttt cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac
    56281 tggcgacaaa actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc
    56341 gggcttcacc gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat
    56401 agacgtcgct gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg
    56461 gctcggcaac ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga
    56521 tatctgcggc aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa
    56581 tgatattggt gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg
    56641 attccagcgc agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag
    56701 aaatagcccc tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca
    56761 gttcggcctg gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct
    56821 ccagcaaagc ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt
    56881 ccagtcgctg ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa
    56941 caaactggcc ttctttcacc agaatggtgt cgatacgccc ggcaattttg ctggcgatat
    57001 ccacttccgt cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg
    57061 ggcgcaacag ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg
    57121 ccagatggcg cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg
    57181 cgtgttacaa ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa
    57241 acgacaaata cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagtgaaaac
    57301 gggtagtaat gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa
    57361 gtgtgatcta taaaactggc tgacaaaacg gtatccatcc tgttggatcg catttcacgc
    57421 ctgttcagtt gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat
    57481 agtaacatta atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat
    57541 caaaggcata agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat
    57601 ctctggccct gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg
    57661 tttactgtca cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc
    57721 accatttccg ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag
    57781 tgcttcaagt ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca
    57841 agagatagcc cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt
    57901 aataatttcc gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt
    57961 tatcggtcct ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc
    58021 cattattttg tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt
    58081 atcctcatca agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt
    58141 ttcatgattt ccagctaata catgaacatt ttcattgata ctcattatgc gttttaacaa
    58201 atcgatgata aattgatcgc cacagatatt actaaatcga tcgccggttt ggtcgccaag
    58261 aaacgtacat ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa
    58321 aacattatct cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc
    58381 attaaagttc tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct
    58441 aaggaaattt tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa
    58501 aactgcaccg actgagccat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc
    58561 aacaggtgaa caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc
    58621 gaatttaata tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga
    58681 ctcagtaact gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac
    58741 aatgctgcac caattaatat cggtattatc aagagggcaa ccgtaatttt tactattaat
    58801 tgtcacaata aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag
    58861 catactatat ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat
    58921 agattgttgg aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat
    58981 tgtcctggca atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc
    59041 tttagtgaag ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact
    59101 gccattagtg cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag
    59161 gaatacatag ttaacatttt atcttatgac atacacctca tattcgatgg ggaaataatt
    59221 atattttgca caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag
    59281 agaaagtccc taaatattgt agaggcgtca tcagtacata cctctacaat cgattaagaa
    59341 aacctatgaa atactggcaa aattaaaaag agataccaca gcactcatga agttttattg
    59401 aggttgaaaa caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat
    59461 cttccaagaa tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa
    59521 tccataatgg ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc
    59581 ttcaggtttt ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg
    59641 tgctatagca ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt
    59701 tttattacat atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg
    59761 aacctgcaaa gcaccgagac aataacattg attttcttca tttcttatta ttccgtgatg
    59821 actggttaaa actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt
    59881 atacgtatca ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa
    59941 attgatttca tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt
    60001 accctccata tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt
    60061 actaaatcta tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt
    60121 taaaaaaaca tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta
    60181 gagcaattta ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa
    60241 ttcacataat aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga
    60301 ataaagtaca gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg
    60361 tgggttaatc acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata
    60421 gctatcaaac ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt
    60481 aaaattaccg ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt
    60541 ataaattatg tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc
    60601 aatcttaact gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt
    60661 aaagataaac gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga
    60721 tggctcacta ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca
    60781 gtgaccatgt tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc
    60841 attatccagc ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg
    60901 gtgttgatga ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt
    60961 aaatatattg tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa
    61021 ataaatgata catgtctgaa agacatattt acgacgatat aaaacaataa tacttatatg
    61081 ttcgctgatt ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca
    61141 tccctggaag acaattgtaa aaaaaaacaa cagaataaga attaatctta ggataaatat
    61201 ttatttatca tggccttttg accgctaaaa aatggcactc ggcacagtaa accccgctat
    61261 agttatgcta tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta
    61321 tcattaatgc acatttcaat attagcaggg atacctgagg agtttaaagt gaacatatat
    61381 atcgggtagc ttttcaaatt aatccctttg attataggct taatttgcat cgcgttaggt
    61441 ggctttgtgc tggaaagttc agggcaacgt cacgggtcat gtgctgattt ctctggcggc
    61501 catatgcctg gcattattca ctaccgcatt tattatcatt tcgcaactca cgcgcggcgt
    61561 taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat
    61621 gatatggggt tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt ttgttgccgg
    61681 ccatgttatt tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg cagcgtcgtc
    61741 cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgacg gaacaccggt
    61801 acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct
    61861 gctcggtttc atttggtcta ttacgctgtt acatagtgcc gacataactc cgcattatgt
    61921 cgccttgggt taaccgcaat ctgtgcctgt ctaattggcc tagtggccac aatcgtccat
    61981 caaacacgta atacgttctc atccaaagag cactggctgt ggtgttattg ggttattttt
    62041 ctcggctcag tcacggtgct gcaggggata tacgtcttag tcagttccga tgcaagcgcc
    62101 cgactggctc ccggcattat tctcatttgc ctcggaatga tctgttacag catattctca
    62161 aaagtctggc tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg
    62221 atgataccgg tatttacctg tctgttttgc ttgtttttgt catcattcct cgcggaaatc
    62281 gcacaaactg agctggacta ctttattcca tctcgggttc tggttggatt aggcgcagta
    62341 tgttttacat tgttctctat tgtttcaata ttagaggccg gttctgcgaa aaaaaattaa
    62401 aatttacata atcccagcgt tattcatatc ttaacgctgg ttttttacga acttagtgaa
    62461 ataatgtaga atataaaatt ataattttat attggagcac atgtgttaat gatcaacccc
    62521 gggatactaa aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt
    62581 aaagatgcat cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc
    62641 gaaactttgg ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa
    62701 aaatacgaat gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa
    62761 ctaccgctac catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga
    62821 tctgttttag catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca
    62881 cgagccttcc agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata
    62941 aaatacattt tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc
    63001 attagaggat tttatacatc ttgataaata ccgctgccct tccccaagca aataatgcat
    63061 actatttaca tcatatataa aatttgcacc tggcacacag ggtaaatgct ttttcgaaat
    63121 ttcagcggcc tctatttgag atttagcgag gccaatctcg ttaagattct ctgaaagata
    63181 taaatcacag gtgtcaatct tacctgattt aaccaatgga taatccgtta tactgtaggt
    63241 atctttaaaa gccacggcaa acatttcaat aataacaatt actaccacaa gcgaaatcaa
    63301 aatgcgaaaa agaggaatac tccttttgcg ctctactttt tcaacaacat cttcttgtaa
    63361 atgagaatca tcataagatt gtttacttat tgttatgttt tcactgatgt aaaagcctac
    63421 tttgggtatc gtaagaatca cattttcttc aacaccaata tttttaaaac ttttgcgtaa
    63481 cagggatata tattggttca atgtgttgtt tgacagatca aatgaaaatc tctccaaaac
    63541 gctatcaatg attgagttcc tgtcaaccgc tccaccatta ctttccagta acactgccaa
    63601 aatacacccc gccgtagctg gaaaatttat ctcatcctgg gtatttacat tcgagagcga
    63661 gcatttgttc ggatcaaaaa taatcgcgtc attgataatg aaaaaacatc tccgctccat
    63721 gcaggcaaca acacaaaata agattttgtc attttagcct attcctggtg gaactgacac
    63781 aagaaactaa aatacaaata taaccctgaa aaagtcatat aaaacttata tccacagaat
    63841 aaaaaatcaa acatgtcagt tttatcaata taaagtatcg caaggcgaaa agtgatgcaa
    63901 gaatactcct aagtaaatga aagcgcacca catgaaatta tcaacacatg taatttcatc
    63961 attaggtgct atcgaaaata actcaggagc tacttaagat gaataatgtc ttactcatga
    64021 gacccgatga ctcagaattt atgatacctg atgactggtc agggctaaac gcaaaaggca
    64081 ttacatacga acaagataat agagtgacag agtcttttct gccttttaaa tctttttatt
    64141 gttcaccggt gtgcacgaca gttgaactca tctcacaaaa taattgtttt gctatgggta
    64201 tccgctacct tctggaaaag aaaagagact taaggctaaa tcactttaaa gccttctctc
    64261 ataaatgcga gacaaaacct gacgtgttaa ttatgaccgg gtgcaccgtc gctgatactt
    64321 atagtatatt ctcacgcatt gttgaagaaa atataatgaa aacgatgaca cctatcgtca
    64381 tcattactgg gcatcactca gaagatcttt gttctctggc atcgaaactg ggaggagtta
    64441 aaataatatc accgacagaa aagattgcaa aaatagaaaa cgctatcaat gatgctttta
    64501 acagcaagcc taaaaaaata agatttttaa cagatatact ctctcctcaa caaattgaga
    64561 ccatgaaaat ggttgcgcag ggtagatccg ttaatgagat ttcagccgca atgaatatta
    64621 gcatcaagac cgtgtatgca cataaagttc gtgctatcaa taagctggga attaagaaaa
    64681 aagtttatga agcgcttttt tataaagcgc taattcagtt caactgatgt aaaaatcacc
    64741 ctcgcttaat aaataatata ttctatgtct ttttttataa gttaaaaaag gcgggatatc
    64801 aaatcccgcc atgaaattaa ctaatcatct gcattattct gcgtagatat aagcctgggt
    64861 acggatggtg taagagtctg gctcaacaac ctgttctttc atatgggaaa cgacgcgata
    64921 ggcaccacct gttccagtat ttttcagcat catataacgc ttaccgccaa tctcttcgga
    64981 ggcagctgaa ggaggatcct gctcaagcgt tgtcagggaa actttcagtt tatggtccgg
    65041 gttattgtca ccagaaatta ctgcatccgc tgaagtactg ctctccgcat aaatatcatc
    65101 cgggaaagtt acagcatagt cgtggctctg cccatcagat gcgccaacaa tacctgttgc
    65161 caaaggcgtc catgcagtta acgtaccgct atatacccct ttcactggtt gaatggtatt
    65221 attaacaacg ataggcttgg tcaaagtaag attagctgac gcttcagtac cctggcttac
    65281 taaagttccc gcctgagaag caaaagctgt ccccgccagc atgattgcta acaatgtttt
    65341 cttcattata ttttctccat gattttcatt tacactgcaa aatgcagttt ctttctttgc
    65401 atttacgtgc tatcgcgaaa atgcttccac caaataatgg aaacatcttc acaaaaataa
    65461 attatgagtt tcttaattta gaaactataa acatgtcgcc gctatctggt ataaccctga
    65521 tgcacttttc ttcgcaggaa gttggtattt tgcgatacag gattgatctt tagaataacc
    65581 ccatttcaca ttaaactcgc cttccgcact caagccagaa acataagcaa taccatcttc
    65641 acctacaatg ccggccatat tttcagtgtc tttgctataa ctaatcacag cgccgaaggg
    65701 gatgggttta ccctctttct gttttaaggt tactaatcct ttatccccgc tcttcactgc
    65761 caatgtcgca ggcacaacgg ctccagctgt cggtacaacc aattgactag tttgtggtaa
    65821 gtcgtaattc ttacctatcg ttttttcatc cagaataaca tcgtctcttc ggtatggctg
    65881 cactggcagt attgcatacc cacgaaaatc agtactcagt cccggccagc cattaacgtt
    65941 aacccccgtt gctccgggtg cctctataag ggctaatgtc ccatccacat tttgaccaaa
    66001 tgtaagtcca tcccttgtaa taagtatccc accgcttaaa tttgcaccta gctgtttcat
    66061 gttttcgctg gagctgtaat ggctactaaa ctgaccatat gatccatttt tgccaacttg
    66121 cacaatgttc atcgtgctct tcggttgtga ctgatgagcg ttagttatat ttgtcgctac
    66181 atcccaccat acaccaccac tattctgacc tgataatgaa acctgatagt tattaccctg
    66241 ctctcccgaa cgactcatct gtgaagacgc ataaatttga ttttcgtcac ccagcgataa
    66301 tggcactgaa atacccacgc ttaatttccg atctactttc catttatgcg atgtcagcaa
    66361 gtcgtttttt gcccagttaa tggagaatgt tatgcctctc cataccgtac tataactcag
    66421 gctggtagac cgcgtgtttc ctgtgccccg ggaagaatta tacctcgtgg tattgaagtt
    66481 caaattcccg atattaccaa ggggctggga taaggatatc gtagagcc
//
LOCUS       DAJTNP010000010        66028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTNP010000010 DAJTNP010000000
VERSION     DAJTNP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="OO160_000717"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="OO160_000717"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5522953.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO160_000718"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO160_000718"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5522954.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO160_000719"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO160_000719"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5522955.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO160_000720"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO160_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5522956.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO160_000721"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO160_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5522957.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO160_000722"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO160_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5522958.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="OO160_000723"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="OO160_000723"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5522959.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7607..10141
                     /gene="mrcB"
                     /locus_tag="OO160_000724"
     CDS             7607..10141
                     /gene="mrcB"
                     /locus_tag="OO160_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5522960.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10361..12604
                     /gene="fhuA"
                     /locus_tag="OO160_000725"
     CDS             10361..12604
                     /gene="fhuA"
                     /locus_tag="OO160_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5522961.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12655..13452
                     /gene="fhuC"
                     /locus_tag="OO160_000726"
     CDS             12655..13452
                     /gene="fhuC"
                     /locus_tag="OO160_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5522962.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13452..14342
                     /gene="fhuD"
                     /locus_tag="OO160_000727"
     CDS             13452..14342
                     /gene="fhuD"
                     /locus_tag="OO160_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5522963.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14339..16321
                     /gene="fhuB"
                     /locus_tag="OO160_000728"
     CDS             14339..16321
                     /gene="fhuB"
                     /locus_tag="OO160_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5522964.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="OO160_000729"
     CDS             complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="OO160_000729"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5522965.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17861..19282
                     /gene="clcA"
                     /locus_tag="OO160_000730"
     CDS             17861..19282
                     /gene="clcA"
                     /locus_tag="OO160_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5522966.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19306..19371
                     /locus_tag="OO160_000731"
     CDS             19306..19371
                     /locus_tag="OO160_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5522967.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19364..19708
                     /gene="erpA"
                     /locus_tag="OO160_000732"
     CDS             19364..19708
                     /gene="erpA"
                     /locus_tag="OO160_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5522968.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19755..20378)
                     /locus_tag="OO160_000733"
     CDS             complement(19755..20378)
                     /locus_tag="OO160_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5522969.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="OO160_000734"
     CDS             complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="OO160_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5522970.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="OO160_000735"
     CDS             complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="OO160_000735"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5522971.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21991..23508
                     /gene="dgt"
                     /locus_tag="OO160_000736"
     CDS             21991..23508
                     /gene="dgt"
                     /locus_tag="OO160_000736"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5522972.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23638..25062
                     /gene="degP"
                     /locus_tag="OO160_000737"
     CDS             23638..25062
                     /gene="degP"
                     /locus_tag="OO160_000737"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5522973.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25217..26373
                     /gene="cdaR"
                     /locus_tag="OO160_000738"
                     /pseudo
     CDS             25217..26373
                     /gene="cdaR"
                     /locus_tag="OO160_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26462..26848)
                     /locus_tag="OO160_000739"
     CDS             complement(26462..26848)
                     /locus_tag="OO160_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5522974.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="OO160_000740"
     CDS             complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="OO160_000740"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5522975.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="OO160_000741"
     CDS             complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="OO160_000741"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5522976.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30751..31545)
                     /gene="map"
                     /locus_tag="OO160_000742"
     CDS             complement(30751..31545)
                     /gene="map"
                     /locus_tag="OO160_000742"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5522977.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31913..32638
                     /gene="rpsB"
                     /locus_tag="OO160_000743"
     CDS             31913..32638
                     /gene="rpsB"
                     /locus_tag="OO160_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5522978.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32896..33747
                     /gene="tsf"
                     /locus_tag="OO160_000744"
     CDS             32896..33747
                     /gene="tsf"
                     /locus_tag="OO160_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5522979.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33894..34619
                     /gene="pyrH"
                     /locus_tag="OO160_000745"
     CDS             33894..34619
                     /gene="pyrH"
                     /locus_tag="OO160_000745"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5522980.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34911..35468
                     /gene="frr"
                     /locus_tag="OO160_000746"
     CDS             34911..35468
                     /gene="frr"
                     /locus_tag="OO160_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5522981.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35560..36756
                     /gene="ispC"
                     /locus_tag="OO160_000747"
     CDS             35560..36756
                     /gene="ispC"
                     /locus_tag="OO160_000747"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5522982.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36945..37703
                     /gene="ispU"
                     /locus_tag="OO160_000748"
                     /gene_synonym="uppS"
     CDS             36945..37703
                     /gene="ispU"
                     /locus_tag="OO160_000748"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5522983.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37716..38573
                     /gene="cdsA"
                     /locus_tag="OO160_000749"
     CDS             37716..38573
                     /gene="cdsA"
                     /locus_tag="OO160_000749"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5522984.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38585..39937
                     /gene="rseP"
                     /locus_tag="OO160_000750"
     CDS             38585..39937
                     /gene="rseP"
                     /locus_tag="OO160_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5522985.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39967..42399
                     /gene="bamA"
                     /locus_tag="OO160_000751"
     CDS             39967..42399
                     /gene="bamA"
                     /locus_tag="OO160_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5522986.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42521..43006
                     /gene="skp"
                     /locus_tag="OO160_000752"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42521..43006
                     /gene="skp"
                     /locus_tag="OO160_000752"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5522987.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43010..44035
                     /gene="lpxD"
                     /locus_tag="OO160_000753"
     CDS             43010..44035
                     /gene="lpxD"
                     /locus_tag="OO160_000753"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5522988.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44140..44595
                     /gene="fabZ"
                     /locus_tag="OO160_000754"
     CDS             44140..44595
                     /gene="fabZ"
                     /locus_tag="OO160_000754"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5522989.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44599..45387
                     /gene="lpxA"
                     /locus_tag="OO160_000755"
     CDS             44599..45387
                     /gene="lpxA"
                     /locus_tag="OO160_000755"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5522990.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45387..46535
                     /gene="lpxB"
                     /locus_tag="OO160_000756"
     CDS             45387..46535
                     /gene="lpxB"
                     /locus_tag="OO160_000756"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5522991.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46532..47128
                     /gene="rnhB"
                     /locus_tag="OO160_000757"
     CDS             46532..47128
                     /gene="rnhB"
                     /locus_tag="OO160_000757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5522992.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47165..50647
                     /gene="dnaE"
                     /locus_tag="OO160_000758"
     CDS             47165..50647
                     /gene="dnaE"
                     /locus_tag="OO160_000758"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5522993.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50660..51619
                     /gene="accA"
                     /locus_tag="OO160_000759"
     CDS             50660..51619
                     /gene="accA"
                     /locus_tag="OO160_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5522994.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51718..53859
                     /gene="ldcC"
                     /locus_tag="OO160_000760"
     CDS             51718..53859
                     /gene="ldcC"
                     /locus_tag="OO160_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5522995.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53916..54305
                     /locus_tag="OO160_000761"
     CDS             53916..54305
                     /locus_tag="OO160_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5522996.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54370..55668
                     /gene="tilS"
                     /locus_tag="OO160_000762"
     CDS             54370..55668
                     /gene="tilS"
                     /locus_tag="OO160_000762"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5522997.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55717..55977)
                     /gene="rof"
                     /locus_tag="OO160_000763"
     CDS             complement(55717..55977)
                     /gene="rof"
                     /locus_tag="OO160_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5522998.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55964..56164)
                     /locus_tag="OO160_000764"
     CDS             complement(55964..56164)
                     /locus_tag="OO160_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5522999.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56330..56875
                     /locus_tag="OO160_000765"
     CDS             56330..56875
                     /locus_tag="OO160_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5523000.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56872..57294
                     /gene="arfB"
                     /locus_tag="OO160_000766"
     CDS             56872..57294
                     /gene="arfB"
                     /locus_tag="OO160_000766"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5523001.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57308..58018
                     /gene="nlpE"
                     /locus_tag="OO160_000767"
                     /gene_synonym="cutF"
     CDS             57308..58018
                     /gene="nlpE"
                     /locus_tag="OO160_000767"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5523002.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58173..58997)
                     /locus_tag="OO160_000768"
     CDS             complement(58173..58997)
                     /locus_tag="OO160_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5523003.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59050..60768)
                     /gene="proS"
                     /locus_tag="OO160_000769"
     CDS             complement(59050..60768)
                     /gene="proS"
                     /locus_tag="OO160_000769"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5523004.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60879..61586)
                     /gene="tsaA"
                     /locus_tag="OO160_000770"
     CDS             complement(60879..61586)
                     /gene="tsaA"
                     /locus_tag="OO160_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5523005.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61583..61987)
                     /gene="rcsF"
                     /locus_tag="OO160_000771"
     CDS             complement(61583..61987)
                     /gene="rcsF"
                     /locus_tag="OO160_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5523006.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62105..62920)
                     /gene="metQ"
                     /locus_tag="OO160_000772"
     CDS             complement(62105..62920)
                     /gene="metQ"
                     /locus_tag="OO160_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5523007.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62960..63613)
                     /gene="metI"
                     /locus_tag="OO160_000773"
     CDS             complement(62960..63613)
                     /gene="metI"
                     /locus_tag="OO160_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5523008.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63606..64637)
                     /gene="metN"
                     /locus_tag="OO160_000774"
     CDS             complement(63606..64637)
                     /gene="metN"
                     /locus_tag="OO160_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5523009.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64825..65400
                     /gene="gmhB"
                     /locus_tag="OO160_000775"
     CDS             64825..65400
                     /gene="gmhB"
                     /locus_tag="OO160_000775"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5523010.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65764..>66028
                     /locus_tag="OO160_000776"
     rRNA            65764..>66028
                     /locus_tag="OO160_000776"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15628 a        16343 c        18197 g        15860 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgaccctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 ggtgcggtaa aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga
      661 cgcggtgacg gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcataccacc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc
     1681 agacggaaac ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2341 gctggtacag cgggatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc
     2521 gcagcaggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaaaataa
     3841 ccgcacgctg gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg
     4021 agccgtattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta
     4381 ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggaggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctctgcg ctcacttcgc ctaaaaatgc cagagtcagg tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcggat
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggctgaat acccgcctgg aagtggttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt
     5341 gatccttgat gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga
     5461 caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt
     5581 tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 ggtgctctgc ccgctgtatg gcgtgttgtc actgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc
     5881 gcgcacgggg cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca
     6001 agcagaacgc gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcatcgc cgtgggcaag atggacgcta tcaactggca aacagcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt
     6661 acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct
     6781 ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac
     6961 gctggaaacg tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag
     7261 gccgctgcaa atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca
     7321 aaaggagatg aaagggcgtt atcccaaaca cgtctggccg gacgacccgg caaacacggc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct ctccctcaag
     7501 ggagagggga ccgatcgagc gcaaattttg agagatatct tcttctgtct tgtaacagaa
     7561 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
     7621 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
     7681 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
     7741 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
     7801 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7861 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc
     7921 agtttatggc cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt
     7981 gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     8041 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8101 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8161 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat
     8221 ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct
     8281 ggtggatact ttgctggcga cagaagaccg tcatttttac gagcatgatg gaatcagtct
     8341 ctactccatt gggcgtgcgg tgctggcaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8401 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg
     8461 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8521 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8581 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8641 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8701 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8761 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgtgg
     8821 tggggtgatc tctcctcagc cagcctttat gcagctggtg cgtcaggagt tgcaggcaaa
     8881 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8941 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     9001 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9061 tgcgatggtc ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9121 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9181 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9241 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9301 ggtggatgcg ttgacacgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9361 gctgcctgcg gttacggaga cctggatcaa actgggcgta ccgaaagatc agttgcatcc
     9421 ggttccggca atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt
     9481 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9541 ggaagatggt aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9601 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9661 gcttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9721 cgtagatacc tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggccg
     9781 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9841 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9901 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9961 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
    10021 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
    10081 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
    10141 acatctaagc gtgaaatacc ggatggcgag ttgccatccg gtaaaataac atcccatcta
    10201 agatattaac cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg
    10261 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
    10321 tcgtttacgt tattattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc
    10381 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
    10441 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
    10501 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc ggcagtcagc
    10561 taccggcact aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac
    10621 cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc
    10681 gggtgtctct gttggtacgc gtggcgcatc caacacctat gaccacctga tcattcgcgg
    10741 ttttgcggca gaaggccaaa gccaaaataa ctatctgaat ggcctgaagt tgcagggcaa
    10801 cttctataac gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg
    10861 cccggtttcc gtgctttacg gtaaaagcag tcctggcggt ctgttgaata tggtcagcaa
    10921 gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt
    10981 ccagactggt tttgacttta gcgatgcgtt ggatgatgac ggtgtttact cttatcgcct
    11041 gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc agcgttatgc
    11101 tattgcaccg gcgttcacct ggcgtccgga tgataaaacc aatttcacct tcctttctta
    11161 cttccagaac gagccggaaa ccggttatta cggctggttg ccgaaagagg gaaccgttga
    11221 gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga agaacaacac
    11281 ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt
    11341 tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg
    11401 ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc
    11461 agcggataaa ggccattatc tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa
    11521 cttctccgtt gatacccagt tgcagagcaa gtttgccact ggcgatatcg accacaccct
    11581 gctgaccggt gtcgacttta tgcgtatgcg taatgacatc aacgcctggt ttggttacga
    11641 cgactctgtg ccactgttca atctgtacaa tccggtgaat cccgatttcg acttcaatgc
    11701 caaagatccg gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt
    11761 ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga
    11821 ctgggcagat caagaatctc ttaaccgcgt tgctgggacg accaataaac gtgatgacaa
    11881 acagtttacc tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa caccttactt
    11941 cagctatagc gaatcgtttg aaccttcttc gcaagttggg aaggatggta atattttcgc
    12001 accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat
    12061 tgtagttact ggtgccgtgt ataatctcac taaaaccaac aacctgatgg cggaccctga
    12121 gggttccttc ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc
    12181 gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga
    12241 atacaccacc gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc
    12301 ttcgctgtgg gctgactata ccttctttga cggtccgctt tcaggtctga cgctgggcac
    12361 cggtggtcgt tatactggct ccagctatgg tgatccggct aactccttta aagtgggaag
    12421 ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca tggcgggctc
    12481 caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa
    12541 cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa cctttcgttt
    12601 ctaatttctc ttttggggca cggatttccg tgcccatttc acaagttggc tgttatgcag
    12661 gaatacacga atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgccc
    12721 gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gaccggtctg
    12781 attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggtcg tcatcagcct
    12841 ccgtcagaag gggagattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg
    12901 tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg
    12961 cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg ctttggtgcg
    13021 gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc gctggcgcat
    13081 cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg
    13141 gcgcaggata gccgttgtct gttgctcgac gaaccgactt cagcgctgga tatcgcccac
    13201 caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt
    13261 gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt cgccctgcgc
    13321 agtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa
    13381 atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc acctgtgagt
    13441 tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta acggcgatgg
    13501 cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcggctatt gaccccaatc
    13561 gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt
    13621 acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca ttgccggact
    13681 cagtgattga cgtcggtttg cgtacagaac ctaaccttga actgctgacc gaaatgaaac
    13741 cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc agaaatgctg gcacgtattg
    13801 cgccgggtcg cggatttaac ttcagtgacg gcaaacatcc gttggcgatg gcgcgtaaat
    13861 cgctgacgga aatggcagat ttacttaacc tgcaaagcgc agcggaaacg catttagcgc
    13921 aatacgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt
    13981 tactgctgac gacgcttatc gatccgcgcc atatgctggt cttcggtcca aacagcttgt
    14041 tccaggaaat tctcgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct
    14101 ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt gatgtgctct
    14161 gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg
    14221 ccatgccgtt tgtccgtaca gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg
    14281 caacgctttc ggcaatgcac tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt
    14341 gagtaaacga attgcgcttt tcccggcgtt attgctggcg ctgttagtga ttgtcgctac
    14401 ggcgctcacc tggatgaact tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc
    14461 ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg
    14521 tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca
    14581 agtactgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact
    14641 ggggattacc gtcaccacgc tctgggcgat ccctagcgcg atggcgagcc agtttgctgc
    14701 gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct
    14761 gtcgccggta acgctgattt tcgcggggtt ggtagtgagc ctttattgcg gcgcaatcaa
    14821 tcagttactg gttatcttcc atcatgacca gttgcaaagc atgtttctgt ggagcactgg
    14881 aacgctgacg caaaccgact ggggcggtgt tgagcgttta tggccgcagt tgctgggcgg
    14941 tgtgatgctg acgttgctgc tacttcgtcc gttaaccctg atggggcttg atgatggcgt
    15001 ggcgcgcaat ctcgggctgg ccttgtcgct tgcgcgtctg gcggcgctgt cgctggcgat
    15061 tgtcatcagt gcgctgctgg tgaacgctgt ggggattatc ggctttatcg ggttgttcgc
    15121 gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc
    15181 gttgattggt gcgctgatcc tctggctttc cgatcaaatc atcctctggc tgactcgcgt
    15241 gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg
    15301 gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggttaacg atcgtgtcgc
    15361 gactgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgt tgttgatggc
    15421 tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc
    15481 gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg
    15541 cgtcatgctg gcggtagcgg gctgtattat tcagcgactg accggcaacc cgatggcaag
    15601 cccggaagtg ctggggatta gctccggcgc ggcgtttggt gtggtgttaa tgctgtttct
    15661 ggtaccgggt aatgcctttg gctggttgtt acccgcaggc agtctcgggg cggcggtgac
    15721 gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact
    15781 ggcggggatg gcgttaagta ccgcgttcac catgcttttg atgatgttgc aggcaagtgg
    15841 tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca acgcgaccga
    15901 tgctcaggtc tggcgcaccg gaattgtgat ggtgattttg ctggcgatta ctccgctgtg
    15961 ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcgcgggcgg tagggatggc
    16021 gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg cgaccgcgac
    16081 gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat
    16141 gggctttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg gtttactgct
    16201 ggtgttcgct gactggtgtg ggcggatggt gctgtttcca ttccagatcc cggcggggct
    16261 gctgtcgacc tttatcggtg cgccatattt tatctatttg ctgagaaagc agagccgtta
    16321 atagaatgcc tcgcccggtg ggcgaggcaa aattatcaca acttcgcgaa cacccgacgt
    16381 gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacagacat aaagcctgct
    16441 tcaaacgctg acggtgccag gtaaacacct tcatccagca tcatatggaa gaaacgctta
    16501 aaacgctcga cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta
    16561 aagaagatac cgaacatacc accgacgtgg ttaacgacca gcggaattcc cgcttcttct
    16621 gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt ttcgtgaatg
    16681 cccggctgcg cgacttcatt cagacaggcg aaacccgctg ccatcgcaat cgggttaccg
    16741 gaaagcgtac ccgcctgata gaccggacct gtcggggcca gcgcatccat tacatcacga
    16801 cgaccaccga atgcgcctac cggcattcca ccgccgatga tttttccgag gcaggttaaa
    16861 tctggcacta cggcgaaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc
    16921 acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg
    16981 aactctggca gcggcggaac acagttcata ttgcctgcca ccggctcgac gataatacag
    17041 gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag aggccagatc gttataagta
    17101 caggttaagg tatgtttggc gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac
    17161 gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct
    17221 tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat ggcgctcatc
    17281 gtcgcctcgg tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc
    17341 accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg
    17401 gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc
    17461 caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc
    17521 gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc
    17581 acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag
    17641 ggttcctgat tcgtagaaaa agtgaatggc tgctattcca tgttattcat aacaagttaa
    17701 atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa
    17761 gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa
    17821 tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc
    17881 tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga
    17941 gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg
    18001 gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc
    18061 gctggtacat actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc
    18121 ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgcccgaag cagggggatc
    18181 ggggatcccg gaaattgaag gggcgctgga agatcaacgt cctgttcgct ggtggcgtgt
    18241 attgccggtg aaattctttg gcgggctggg gacactcggc ggaggcatgg ggctagggcg
    18301 cgaaggacca accgtgcaga tcggcggcaa tatcagtcgc atggtgcttg atattttccg
    18361 cctgaaaggt gatgaagctc gccatacgct gctggcaacc ggtgctgctg cggggctggc
    18421 tgcggccttt aacgcgccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca
    18481 gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat
    18541 tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgatgc
    18601 gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc
    18661 tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa
    18721 tattaccaaa tgggtactga tggggggggc gattggcggt ctgtgtggat tgctggggtt
    18781 tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa
    18841 tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg gtcattacca ccttactctg
    18901 cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct
    18961 gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc tggaggcggg
    19021 gacgtttgct attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac
    19081 ggggatcatt ctggttctgg agatgaccga taactaccag ctcattttgc caatgattat
    19141 taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaacctc tatactcggc
    19201 gattcttgcg cgcacgctgg caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc
    19261 atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt
    19321 gggttagaat ttgcccgatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc
    19381 actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga
    19441 agataacccg aatctgaaat tacgcgtgta tatcaccggt ggcggttgca gcggcttcca
    19501 gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg
    19561 cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta
    19621 caccgaagga ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg
    19681 cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg
    19741 cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc
    19801 caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt
    19861 tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag
    19921 gcagttgcgt agatttctgt acgtaaaatc atggggattt cgcggaccag tacatcacga
    19981 atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct
    20041 tccgcattaa aggctttatt cacgctaatg ccgacaaaca ccgccagacc aacggcgtcc
    20101 agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc
    20161 atgctggtga ccatggcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg
    20221 tgatccagcg ccatgtcgcg aattgttccg tcgcctactg cggtaaccac gcccagtacc
    20281 agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccgta gatggcaaat
    20341 accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc
    20401 tgaaaaggtc aggcactaat ctacctgtga aagcgcatta cagagctgtt gtgctgcgag
    20461 gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt
    20521 gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac
    20581 aatagcctgt ggcgagcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg
    20641 gctgtcttta aagatgtttt ctccgccaca aacttcgagt acctggttct gaatcgactc
    20701 ttttccactg gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt
    20761 atcagcatat tgcgctttca attgcacgta ctgatccagc agggattgcg cggcttgttc
    20821 ggccttgtct ggttgcggac tccagggggc cagttgacgt aacgcattgg caatttgttc
    20881 aatgcttgtc gcatcgaccc acatcacttt tattcccagc gaagccagct ggtcaacctg
    20941 ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg cgacaatgcg
    21001 ttccagattc atcccctgcc aggtggaaac ctgctcaatc ttttgcgctt gtggaggata
    21061 gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt
    21121 ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagtggcg cgagaaaaga
    21181 cagggcgacc agcgccctga acagtgattt agccatgtgc cagtttctgc accagtgact
    21241 caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag
    21301 actgttgatc ggccacgtcg gagatggcgc gtacgacaac aaacgggacg ttgaaattgt
    21361 ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt
    21421 ggcggatttt cgccagacca acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc
    21481 cacgtacagc gttaagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat
    21541 cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc gtattcataa ccaaatgccg
    21601 tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagcg
    21661 ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca
    21721 acagcagagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa
    21781 cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac
    21841 ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc cattgcacca atgatgccga
    21901 ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg
    21961 gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata
    22021 aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg
    22081 atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa
    22141 aagacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg
    22201 atggaagtcc agcaggtggg acgctacatc gccaaagaaa ttttaagccg tctgaaagag
    22261 cttaaattgc tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt
    22321 gagatgtcat gcctgatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagca
    22381 gcgataaatg actggtttcg ccaacgtttg cacccggaag atgccgaaag ccagcctctg
    22441 actgacgatc gctgcagcgt ggcggcacta cgtttacggg acggggaaga accgcttaac
    22501 gagctgcggc gcaagattcg tcaggactta tgtcattttg aggggaatgc acaaggcatt
    22561 cgtctggtgc atacattgat gcggatgaat ctcacttggg cacaggttgg cggtatttta
    22621 aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg agacacatca ctatttaatg
    22681 aaaaagccgg gttattatct ttctgaagaa gcctatattg cccgcttgcg taaagaactt
    22741 aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc
    22801 tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag
    22861 ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct cttttcgctg
    22921 gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat
    22981 cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa
    23041 cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa
    23101 gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg
    23161 tttagccatc cggatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta
    23221 ttagagattt atcgtccttt attaagcctg tcgttatcag actttactga actggtagaa
    23281 aaagaacggg tgaaacgttt ccctattgag tcgcgcttat tccacaaact ctcgacgcgc
    23341 catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg
    23401 ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac
    23461 ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt
    23521 aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact tcgggctata
    23581 aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg
    23641 aaaaaaaaca cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg
    23701 ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt
    23761 gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca
    23821 accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg
    23881 ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt
    23941 aatggcggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc
    24001 gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc
    24061 cagctgagcg atggtcgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat
    24121 atcgcgctga tccaaatcca gaacccgaaa aaactgaccg caattaagat ggcggattct
    24181 gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg tctgggcgag
    24241 acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac
    24301 gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgctggtt
    24361 aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac
    24421 atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg
    24481 gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaattccgaa
    24541 ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct
    24601 aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt
    24661 aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc
    24721 aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag
    24781 cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag
    24841 atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaagac gggcactccg
    24901 gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gtgcgaacca gcaggcagtg
    24961 aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac
    25021 attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc
    25081 tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt
    25141 catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt
    25201 gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt
    25261 ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat
    25321 tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat tgctggtact
    25381 ttcacaggga cgagtcgtcg atatcgatga cgcggtagca cgtcatctgc acggtgtgcg
    25441 gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac
    25501 aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat
    25561 gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc gggaagaact
    25621 ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttctgaatg ggcgcaacgg
    25681 ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag
    25741 cttggcgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc
    25801 gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg
    25861 ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga acaactgatt
    25921 acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc
    25981 ggtcctggca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa
    26041 cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc
    26101 gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa
    26161 gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg ccacaatgtg
    26221 caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt
    26281 aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg
    26341 tatgtggcgt tacaactgga tgaagagcgg tagattatgc gttaaggttg aggcggcgct
    26401 ggctcattcc cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg
    26461 attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga
    26521 ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt
    26581 cacgctgaca gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct
    26641 ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat
    26701 atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca
    26761 gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa
    26821 tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg
    26881 cttattttag catttggctt ggcccgcccg gctcgactgt gatcgcagaa agcctttttc
    26941 ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt
    27001 ccatgggatg gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa
    27061 gatgaaaaag tatttaaaaa agggggagga agaatgagta ggacgaatta ttctgagcca
    27121 gaaagcatac gggcagcatc atgctgcccg tatatgtact cttaatcgat ggtacgcagc
    27181 agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca
    27241 cagtagaggc tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc
    27301 gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga
    27361 ccaatgtata cgcccataga gatgacggaa ccttcttcga caatcacccc ttcaaccact
    27421 tcagagcgtg cgccaatgaa gcagttatct tcaataatgg tcgggttagc ctgcagcggc
    27481 tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca
    27541 caagaaccga cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg
    27601 ttaacgtaag acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc
    27661 gctggtggca caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat
    27721 ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta
    27781 atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca
    27841 attttttccg ctacacgcag tgcgccggaa tccagcaggg cgatcacctg atttaccgct
    27901 tcgcgggtaa cggtgtctgc attggctggc gtgatctcgg cacggcgttc aaaagcggtt
    27961 tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac
    28021 cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg
    28081 ttattaagcg cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca
    28141 atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact
    28201 cgcgccagca gtccaggttg gtcgagggcg atcagttcga ggaacgattt gcggtcggta
    28261 tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg
    28321 cgacggggct gcggtggctg ccagctactt tgcgtcagca cctgctccag accaaaccga
    28381 ataacctcat ggcgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta
    28441 tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg
    28501 cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa
    28561 atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca
    28621 tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag
    28681 ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc
    28741 agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt
    28801 tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc
    28861 ttccagctat tccacagtgt ttcgttggtg gcgcaaatgt cagccactgt caggcatacc
    28921 agatagcgca gacgattttc cgtttgaact tcttcggcaa actgcttgat gacttcaggg
    28981 tcctgaatat cgcggcgttg tgctgtcacc gacatcagca agtgctggcg aaccagccag
    29041 gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg cactacatcc
    29101 tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg
    29161 gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg
    29221 cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt
    29281 tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat
    29341 tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagtcc gcgccgcacc
    29401 gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttgcggaat attacacagc
    29461 ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg
    29521 gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc
    29581 ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg
    29641 agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc
    29701 agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc
    29761 tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga
    29821 tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatacgc
    29881 cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac
    29941 ccgaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg
    30001 tgaatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaaggtt gtagctggtg
    30061 ccatggtaac gctgatggcg ctggttctgt tcttcaactt tcgctgcgta gaacttgtcg
    30121 gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg
    30181 ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc
    30241 aacatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc
    30301 agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa
    30361 atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggca
    30421 accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc
    30481 tggtctatga actcggtacg tgcctcaatc aactgttctg cagagatccc attgtcaaag
    30541 gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccaca gacggttaat
    30601 tcatcacggg gccaggcgca tggattttgc ggttgaccgg gcagggtgga gagagcggtg
    30661 tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca
    30721 ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat
    30781 ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca caatagtatg
    30841 ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt
    30901 gcggatctct tttttacccg cgttgaccat tggctcaatg gtgaacgtca tccccggttt
    30961 cagcacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc
    31021 gcgaccaata ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa
    31081 tttctgaatc gccgcaccga tttcgcgcag attaatgcct ggttttacca tgcgtagcgc
    31141 caggtacagg ctttcttgcg tgatgcggca caggcgttcg cccatgatgg tcggcttacc
    31201 gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat
    31261 gttaacgata tcgccatctt tcagcagctt agcatcgtcc gggataccgt ggcacaccac
    31321 ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac
    31381 cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac
    31441 gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac
    31501 gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca
    31561 gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa
    31621 ttgagaatca ttctgaattt cgccaaacgt gccactgaag gttttctata atagaaaatt
    31681 cgacgtctga tgctgtacac agcgccaaca attattggtg tccacgacgt atttgtggta
    31741 taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct
    31801 cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt
    31861 aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac
    31921 tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg
    31981 gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct
    32041 tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgtttctcg
    32101 caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc
    32161 tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa
    32221 ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga
    32281 cggtactttc gacaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa
    32341 actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt
    32401 aatcgatgct gaccacgaac acattgctat caaagaagca aacaacttgg gtattccggt
    32461 atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa
    32521 cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga
    32581 aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata
    32641 aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc
    32701 ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg
    32761 cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct
    32821 gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc agatcacatc
    32881 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    32941 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    33001 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    33061 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33121 gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa
    33181 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33241 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt
    33301 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33361 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33421 agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac
    33481 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    33541 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    33601 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    33661 ttcatccgtt tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    33721 gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct
    33781 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    33841 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    33901 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    33961 gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag
    34021 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    34081 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34141 cgaccgtaat gaacggcttg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34201 gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta
    34261 tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct
    34321 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34381 aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagatcca accgcaacca
    34441 tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg
    34501 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac
    34561 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    34621 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg
    34681 ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt
    34741 acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct
    34801 aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    34861 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    34921 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    34981 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    35041 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    35101 ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35161 cctccgatct cggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35221 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35281 cgcgtgttgc agtacgtaat gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35341 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35401 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35461 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    35521 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    35581 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    35641 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    35701 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    35761 atgctacagc aacaggggag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    35821 atggcagcgc ttgaggaggt tgatcaggtg atggcagcca ttgttggtgc tgctgggctg
    35881 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    35941 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    36001 ccggtcgata gcgaacataa cgctattttt cagagtttac cgcaacctat ccagcataat
    36061 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36121 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcttgc
    36181 cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac
    36241 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36301 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36361 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36421 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36481 gccgcaccgg attatgagcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    36541 ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    36601 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    36661 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgagtgaa
    36721 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    36781 cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt
    36841 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    36901 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    36961 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    37021 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg
    37081 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc
    37141 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37201 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37261 tgcgtattat tggcgatacc agtcgtttta actcgcgttt gcaagaacgt attcgtaaat
    37321 ctgaagcgct aacagccgga aataccggtc tgacgctgaa tattgcggcg aactacggtg
    37381 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37441 tgcaaccaga tcagatagat gaagagatgt taaaccagca tgtctgtatg catgaactgg
    37501 cccctgtaga tttagtaatt aggactgggg gagagcatcg cattagtaac tttttgcttt
    37561 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    37621 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    37681 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    37741 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    37801 ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    37861 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    37921 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    37981 tttgggcttc gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    38041 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    38101 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca
    38161 gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac tccggcgcat
    38221 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38281 cctggcaagg ctttataggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca
    38341 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38401 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38461 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    38521 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    38581 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    38641 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    38701 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    38761 tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    38821 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc
    38881 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    38941 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat
    39001 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    39061 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39121 cattacagta gcgccatttg acagcgacca acggcgggat gtaaagctcg atttacgtca
    39181 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39241 gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg
    39301 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39361 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39421 ggggagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat
    39481 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    39541 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    39601 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    39661 tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    39721 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    39781 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt
    39841 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    39901 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    39961 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    40021 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    40081 gttggtgcgg ccctcctcag tatgccggtg cgtacaggcg acacggttaa tgatgaagat
    40141 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40201 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40261 ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt
    40321 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40381 gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg
    40441 ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag
    40501 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    40561 ctgcgtgacg aagtgccgtg gtggaatgtg gtaggcgatc gtaaatacca gaaacagaaa
    40621 ctggcgggcg atcttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc
    40681 aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    40741 aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    40801 gggcactccg ctgaaattga gcaactgact aagatcgagc cgggcgagct gtataacggc
    40861 actaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc
    40921 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    40981 gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    41041 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggttgggg
    41101 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41161 gataccgata cccaacgtgt tccgggtagc ccggaccagg ttgatgtagt ctacaaggtg
    41221 aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41281 agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc
    41341 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41401 accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41461 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    41521 attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    41581 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc
    41641 tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    41701 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    41761 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    41821 gtgccgatcg atgacgatca caaatgggtt gttctgggtc gtacccgctg gggttatggt
    41881 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    41941 accgtgcgtg gcttccagtc caataccatt ggtccgaaag cagtttactt cccgcatcag
    42001 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    42061 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42121 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttcattcttc
    42181 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42241 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42301 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42361 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42421 agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa
    42481 ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc
    42541 tgcaggtctt ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt
    42601 caatatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga
    42661 aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa
    42721 aatgaaaaaa ctgcagtcca tgaaagcggg cagcgatcgc accaaactgg aaaaagacgt
    42781 gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg
    42841 tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt
    42901 tgccaacagc caggatatcg atctggttgt tgatgcaaac gccgttgctt acaacagcag
    42961 cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat
    43021 tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt
    43081 catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa
    43141 cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca tgacccagga
    43201 cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc
    43261 gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc
    43321 ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat
    43381 tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa
    43441 aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat
    43501 ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg
    43561 ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg
    43621 cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactgt
    43681 tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg
    43741 cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta
    43801 ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac
    43861 ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg
    43921 cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga
    43981 tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc
    44041 catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt
    44101 tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact
    44161 ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat
    44221 cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat
    44281 gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg
    44341 gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg
    44401 ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct
    44461 ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt
    44521 aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt
    44581 agccgggagg cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg
    44641 gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc
    44701 catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact
    44761 aaaattggcc gcgataatga gatttatcag tttgcctcca tcggcgaagt taaccaggat
    44821 ttgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa
    44881 agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac
    44941 aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt
    45001 ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc
    45061 ggcatgaccg cagtccatca gttctgcatc attggtgcgc atgtgatggt tggcggctgc
    45121 tccggtgtgg cgcaggacgt ccctccttat gttattgcgc agggtaacca cgcaacgccg
    45181 ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct
    45241 atccgcaatg cgtataagct aatttatcgt agcggtaaaa cgctcgatga agtgaaaccg
    45301 gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca
    45361 cgctcaacgc gcggtctgat tcgttaatga ctgaccagcg tccattaacg attgccctgg
    45421 tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac
    45481 gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg
    45541 aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc
    45601 tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag
    45661 atgtttttgt tggtattgat gcgcccgact tcaatattac tcttgaaggt aacctcaaaa
    45721 agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac
    45781 gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag
    45841 cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca
    45901 tgccgttaga tccagataaa aatggtgccc gtgatgtgct ggggatccct tacgatgccc
    45961 actgtctggc attgttgccg ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt
    46021 tcttgaaaac tgcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccgc
    46081 tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc
    46141 tgtcggttca tttgctggat ggaatgggcc gtgaggcgat ggtcgccagc gatgcggcac
    46201 tactggcgtc ggggacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg
    46261 gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg
    46321 tctcgctgcc aaatctgctg gcgggcagag agttagtcaa agagttattg caggaagagt
    46381 gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc
    46441 acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc
    46501 aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca
    46561 gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc
    46621 tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag
    46681 cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct
    46741 gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc
    46801 gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa
    46861 ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggggata gccgcgtacc
    46921 agaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct
    46981 ggatattgtt ttcccgcaat atggttttgc ccaacataaa gggtatccaa ccgcttttca
    47041 tctggaaaaa ctggctgaat atggcgcgac tgaacatcat cgtcgcagct ttgggcctgt
    47101 caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct
    47161 gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga
    47221 tggcttggcc aaaaccgcac cgctggtaaa aaaggcggcg gcgttgggta tgccagcact
    47281 ggcgatcacc gatttcacca acctttgcgg tctggtgaag ttctacggag cgggacatgg
    47341 cgcagggatt aagcctatcg tcggggcaga ttttaatgtc cagtgcgacc tgctgggtga
    47401 tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga atctggcgtt
    47461 gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga
    47521 ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcgggcgca tgggcgacgt
    47581 cggacgcagt cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg cgttttatga
    47641 agaacacttc ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga
    47701 aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac
    47761 taacgatgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat
    47821 ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata
    47881 tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atcccagaag cccttgccaa
    47941 caccgttgag atcgccaaac gctgtaacgt aactgtgcgt cttggtgaat acttcctgcc
    48001 gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaagg
    48061 cctggaagag cgtctggcat ttttattccc tgacgaggaa gaacgtctta aacgccgccc
    48121 ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg gcttcccggg
    48181 ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg
    48241 gccaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct
    48301 cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat
    48361 gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg aacacgtggc
    48421 ggacatgtac ggtcgtgatg cggtatcgca gattatcacc ttcggtacga tggcggcgaa
    48481 agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgaccgtat
    48541 ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc
    48601 gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg
    48661 caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc
    48721 gaccaaaatt accgattttg cgccgcttta ttgcgatgaa gagggcaaac atccggtcac
    48781 ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt
    48841 gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa
    48901 tggcgagcca ccgctggata tcgccgcgat cccgctggat gacaagaaaa gcttcgacat
    48961 gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct
    49021 gatcaagcgt ctgcaacctg actgcttcga agatatgatc gcactggtgg cactgttccg
    49081 ccctggtccg ttgcagtcag ggatggtgga taactttatc gaccgtaaac atggtcgtga
    49141 agagatctcc tatccggacg tgcagtggca gcatgaaagc ctgaaatcgg tactggagcc
    49201 aacctacggc attatcttgt atcaggaaca ggtcatgcag attgcccagg tgctttctgg
    49261 ttataccctc ggtggcgcgg atatgctgcg ccgtgcgatg ggtaagaaaa agccggaaga
    49321 gatggctaag cagcgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgccga
    49381 actggcgatg aaaatcttcg acctggtgga gaaattcgcg ggttacggat ttaacaaatc
    49441 gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cgcactatcc
    49501 ggcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg
    49561 cctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg
    49621 tctttaccat ttccacgtca acgacgatgg cgaaatcgtg tatggtattg gcgccatcaa
    49681 aggggtaggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt
    49741 ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct
    49801 ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat
    49861 gaactcgctg ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg
    49921 tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc
    49981 cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct
    50041 gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg
    50101 cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacgg ctgcggggct
    50161 cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct
    50221 ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca
    50281 gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt
    50341 cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa
    50401 atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa
    50461 ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta
    50521 tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag
    50581 cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt
    50641 tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga
    50701 gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat
    50761 taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat
    50821 cttcgccgat ctcggtgcat ggcagattgc gcaactggca cgccatccgc agcgtcctta
    50881 taccctggat tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgtgc
    50941 gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat
    51001 gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat
    51061 gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa
    51121 gatgcctatc atcaccttta tcgacacccc gggggcttat cctggcgtgg gcgcagaaga
    51181 gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc ttggcgtacc
    51241 ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg
    51301 cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc
    51361 gtccattctg tggaagagcg ccgacaaagc gccgctggcg gctgaagcga tgggtatcat
    51421 cgctccgcgt ctgaaagagc tgaaactgat cgactccatc atcccggaac cgctgggcgg
    51481 cgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct
    51541 ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat
    51601 gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt
    51661 tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg
    51721 aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa
    51781 ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat
    51841 ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag
    51901 tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc
    51961 ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg
    52021 ttttttgaat atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc
    52081 aacgaatatc ttgataacat tacgccgccg ttcacgaaag ccttgtttac ctacgtcaaa
    52141 gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc
    52201 ccggttggct gtctgtttta tgattttttc ggcgggaata cccttaaggc tgatgtctct
    52261 atttcggtca ccgagcttgg ttcgttgctt gaccacaccg ggccacacct ggaagcggaa
    52321 gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaatatcg
    52381 acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac
    52441 cgcaactgcc ataaatcact ggcgcatctg ttgatgatga acgatgtagt gccagtctgg
    52501 ctgaaaccga cgcgtaatgc gttggggatt ctggggggga tcccgcgccg tgaatttact
    52561 cgcgacagca tcgaagagaa agtcgctgcc actacgcaag cacaatggcc ggttcatgcg
    52621 gtgatcacca actccaccta tgatggtttg ctctacaaca ccgactggat caaacagacg
    52681 ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtatactca ttttcatcca
    52741 atctaccagg gtaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt tttcttcgaa
    52801 acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcactgat ccacattaaa
    52861 ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc
    52921 agttatccca ttgttgcttc ggttaagacg gcggcggcga tgctgcgtgg taatccgggc
    52981 aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg
    53041 ctgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa
    53101 gccgaatgca ggcccgttgc gcctggcgaa caatggcacg gctttaacga tgcggatgcc
    53161 gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag
    53221 ggcaatatga gtgaggaggg gatcccggcg gcgctggtag caaaattcct cgacgaacgt
    53281 gggatcgtag tagagaaaac cggcccttat aacctgctgt ttctctttag tattggcatc
    53341 gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaagcg ctcttacgat
    53401 ctcaacctgc ggatcaaaaa tatgctgccc gatctctatg ctgaagatcc cgatttctac
    53461 cgcaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    53521 ctttccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat
    53581 caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgctgga acaactggtc
    53641 ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca
    53701 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    53761 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    53821 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    53881 cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc
    53941 atattgcgat tattgcgacg gattatgcgg tgagcaaagc tttctactgc gatattcttg
    54001 gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg
    54061 cgcttaatgg gcaatatgtg attgagcttt tctcttttcc gttcccgccg gagcgcccca
    54121 gccgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg
    54181 cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca
    54241 cgcaaaaacg cttcaccttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc
    54301 agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat
    54361 cgcaccatca tgacactcac gctcaataga caacttctca cctcacgcca gattctggtg
    54421 gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggacg
    54481 gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc acggtttaag tgccaatgcc
    54541 gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc
    54601 gaacgcgtac aacttgcgca agaggggctg gggattgagg cccaggcgcg gcaggcacgt
    54661 tatcaggcat ttgcccgtac cctattgccc ggcgaagtgc tggtcaccgc gcaacatctc
    54721 gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt
    54781 tcggctatgg cggaagtttc ggagtttgct ggaacacggc ttatccgccc gttgcttgcc
    54841 cgcacgcggg gggaactgga gcagtgggca ttggcgcatg gtttacgctg gattgaagac
    54901 gaaagtaatc aggacgactc atacgatcgt aactttctgc gcctgcgcgt agtgccgtta
    54961 ttgcagcagc gttggccgca ttttgcggaa gcaacggccc gcagcgccac actttgtgct
    55021 gaacaagaga gtctgttgga tgaactgctg gcggatgatt tagcacactg ccaaacgtcg
    55081 caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgtcg cgcggcgatt
    55141 attcgccgct ggctggcagg gcagaatgca ccgatgcctt cccgcgacgc gctggtgcgc
    55201 atctggcagg aagtggcgct ggcgcgggaa gatgcttccc cctgtttacg tttgggcgcc
    55261 tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac cgggcaaagc
    55321 gaaaccattg tgctgtggca gacgtggcta caaccgctgg aattaccggc ggggctggga
    55381 accgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc
    55441 gtgcgtttca aagcgccagg attgctgcat attgtcgggc gcaacggcgg acgtaagctg
    55501 aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac gccactgctg
    55561 ttttatggag aaacgctgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct
    55621 gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga
    55681 taagacgcat caaacgtcgc atccggcgaa agtcaatcag gattcgctta ccaccaccgt
    55741 accgatttca gggtggctaa agctggtaat tttatccaga cgcagctcgc gggtttcgcc
    55801 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcac tggcttttgc
    55861 ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag
    55921 ctcaagatta tcgtaatcat cacaattaat tggttgatac gtatcattca ttgacataat
    55981 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    56041 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    56101 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    56161 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    56221 ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa
    56281 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    56341 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    56401 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgctt
    56461 gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    56521 tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct
    56581 ggggctgccg gatgagcgac gaattaagaa agcctgcacc caggccgcag aagtggcgct
    56641 gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt
    56701 gcagtttgcc aatctttccg tctggtatct ggacgatgaa caactggcga aagtaagcgc
    56761 ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggtgtta tctggttatc
    56821 ggatgataag aataatctgg aagttaactt aaccgcctgg caacaacctt catgattgtg
    56881 atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    56941 ggcgctggcg ggcagcatgt taataagacc tcaacggcta ttcatctgcg ttttgacatt
    57001 cgggcgtcca gcctgccaga gtattacaaa gagcgtctgc tcgccgccag ccatcatttg
    57061 atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    57121 cgcgaagccg ctctggcccg gctggtggca gtgattaaag atttaacaaa agaacaaaaa
    57181 gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    57241 caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga ataaaaagaa
    57301 ggaatggatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct
    57361 gatgggatgt aataatcggg ccgaagtcga tacgctttct ccggcgcagg ctgccgaact
    57421 gaaaccgatg ccgcaaagtt ggcgcggcgt gctaccgtgt gccgattgcg aaggaatcga
    57481 aatctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc
    57541 tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct
    57601 ggtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga
    57661 gatgctcgat cgtgaaggca atccaattga atcgcagttc aactatacgc tggaaccggc
    57721 acaatccagt ttacctatga cgccgatgac cctgcgaggc atgtattttt atatggctga
    57781 tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga
    57841 gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt
    57901 agaaggtcac tttacgcttg aggctaatcc ggatactggt gcgccaacca aagtattagc
    57961 gcccgatacg gcgggtaaat tttacccgaa taaggattgc agtagtttgg ggcagtaaac
    58021 ctgcttaagg cagaaacaaa cgcaaaattg cctgatgcgc tgcgcttatc aggcctacag
    58081 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
    58141 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
    58201 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
    58261 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
    58321 tgcgccagtg taagtggatg cccggatttg gcaaatgcat cagtgacaaa ttccgaacaa
    58381 aaccacgact ttttgtctcc ttcgcacaca ctgcttaact gcgctttcgc caggtcgctg
    58441 acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc
    58501 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
    58561 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
    58621 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
    58681 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
    58741 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
    58801 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
    58861 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg
    58921 ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg ggagaagcag gcggcagtac
    58981 gcctttggtt tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc
    59041 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
    59101 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
    59161 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
    59221 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
    59281 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
    59341 cacctggaac ggcgcgatag cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc
    59401 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
    59461 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
    59521 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
    59581 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
    59641 atcccagttg ataccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
    59701 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
    59761 acccggaccg gctttaacca cagcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
    59821 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
    59881 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
    59941 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
    60001 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
    60061 tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
    60121 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
    60181 ctgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
    60241 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
    60301 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
    60361 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
    60421 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
    60481 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc
    60541 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
    60601 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
    60661 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
    60721 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
    60781 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta
    60841 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
    60901 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
    60961 taggttttgc ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact
    61021 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
    61081 acctctgcgg taaaactcac cgccatctct gcagctggcg cactttgcgc atagctggca
    61141 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
    61201 ccatcgacca gatccagact gccgagcttc agaatcacgc tgcctttatg gcaaacaacc
    61261 tctttcagct ctaccagcga catgccaatt gggttagggc ggaaagtaga gcgtgttgcg
    61321 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
    61381 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaatgc ttccaggccg
    61441 cgaacggcgt cggcctggtt gtaggaagct atgagatgca gttcgccgtt ggcgcttttt
    61501 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
    61561 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
    61621 cagcctgacg atagcagccc ggcgtaccgc tggtgacttc gcagctatgc agtaatacag
    61681 cattggcttt cattttagag gcattgattt gcatctgctt acgtgcggtt ggaatgctcg
    61741 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
    61801 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
    61861 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
    61921 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
    61981 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
    62041 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
    62101 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
    62161 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
    62221 gcgttacgat caggtttacg tatggggagt ctttatcttc gacaaagata ccgtctttcg
    62281 ccggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
    62341 cgtcgtccag agaacgcggc agttgcggtg cttccagttc aacaattttc agattttttg
    62401 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
    62461 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
    62521 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
    62581 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
    62641 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
    62701 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
    62761 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
    62821 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
    62881 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
    62941 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcaa
    63001 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
    63061 tgtagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
    63121 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
    63181 ccggcaacgc ttccggtaac agcactttac agacgatctg cattggcgtg gcacccattg
    63241 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
    63301 caataaacgg tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg
    63361 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
    63421 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
    63481 gccccggacg cgtgacataa agcagaacgc caaccggcag accaatcaca aacccaaaaa
    63541 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca
    63601 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
    63661 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaatttaacg
    63721 ccaccggcgt agtccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
    63781 gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggc
    63841 acgcagtcag taaatggctc cgtttgcaga cgttcctggt aatcttccgg gatatccaga
    63901 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
    63961 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
    64021 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
    64081 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
    64141 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
    64201 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
    64261 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
    64321 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
    64381 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt caattcctgg
    64441 ccatcgacca gcacgctacc ctcggttggg cgctccagca agtttacaca acgtataagc
    64501 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
    64561 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
    64621 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgta gcatctcgaa
    64681 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
    64741 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
    64801 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
    64861 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
    64921 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
    64981 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
    65041 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
    65101 ccgcatcatc cgcaggggag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
    65161 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
    65221 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
    65281 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
    65341 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga
    65401 ttaaaagatg aacggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
    65461 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    65521 ggttgaagag aaaaaatcct gaaattcagg gttgactctg aaataggaaa gtgtaatata
    65581 cgccacctcg cgacagtgag ctgaaagctg cgtcgcaact gctctttaac aatttatcag
    65641 acaatctgtg tgggcactcg aagatacgga ttcttaacgt cgcaagacga taaatgaata
    65701 ccaagtctca agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact
    65761 tttaaattga agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg
    65821 caagtcgaac ggtaacagga agcgcttgct gcttcgctga cgagtggcgg acgggtgagt
    65881 aatgtctggg aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc
    65941 ataacgtcgc aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga
    66001 tgggattagc ttgttggtgg ggtaacgg
//
LOCUS       DAJTNP010000011        64184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTNP010000011 DAJTNP010000000
VERSION     DAJTNP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            122..565
                     /locus_tag="OO160_000777"
     CDS             122..565
                     /locus_tag="OO160_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5523011.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            801..1618
                     /gene="ybhA"
                     /locus_tag="OO160_000778"
                     /pseudo
     CDS             801..1618
                     /gene="ybhA"
                     /locus_tag="OO160_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO160_000779"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO160_000779"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5523012.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO160_000780"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO160_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5523013.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO160_000781"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO160_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5523014.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO160_000782"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO160_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5523015.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO160_000783"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO160_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5523016.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO160_000784"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO160_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5523017.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO160_000785"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO160_000785"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5523018.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO160_000786"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO160_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5523019.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO160_000787"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO160_000787"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5523020.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO160_000788"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO160_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5523021.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO160_000789"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO160_000789"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5523022.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO160_000790"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO160_000790"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5523023.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLSAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO160_000791"
     CDS             13924..14304
                     /locus_tag="OO160_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5523024.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO160_000792"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO160_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5523025.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO160_000793"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO160_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5523026.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO160_000794"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO160_000794"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5523027.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO160_000795"
     tRNA            complement(17405..17480)
                     /locus_tag="OO160_000795"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO160_000796"
     tRNA            complement(17484..17559)
                     /locus_tag="OO160_000796"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO160_000797"
     tRNA            complement(17611..17686)
                     /locus_tag="OO160_000797"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO160_000798"
     tRNA            complement(17689..17764)
                     /locus_tag="OO160_000798"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17900..17975)
                     /locus_tag="OO160_000799"
     tRNA            complement(17900..17975)
                     /locus_tag="OO160_000799"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17940..17942),aa:Lys,seq:ttt)
     gene            complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO160_000800"
     CDS             complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO160_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5523028.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO160_000801"
     CDS             complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO160_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5523029.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO160_000802"
     CDS             complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO160_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5523030.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO160_000803"
     CDS             complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO160_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5523031.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO160_000804"
     CDS             complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO160_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5523032.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO160_000805"
     CDS             complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO160_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5523033.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO160_000806"
     CDS             complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO160_000806"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5523034.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO160_000807"
     CDS             complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO160_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5523035.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO160_000808"
     CDS             complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO160_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5523036.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO160_000809"
     CDS             complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO160_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5523037.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO160_000810"
     CDS             complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO160_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5523038.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO160_000811"
     CDS             complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO160_000811"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5523039.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO160_000812"
     CDS             complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO160_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5523040.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32633..33355
                     /gene="mngR"
                     /locus_tag="OO160_000813"
     CDS             32633..33355
                     /gene="mngR"
                     /locus_tag="OO160_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5523041.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO160_000814"
     CDS             complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO160_000814"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5523042.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO160_000815"
     CDS             complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO160_000815"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5523043.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO160_000816"
     CDS             complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO160_000816"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5523044.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO160_000817"
     CDS             complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO160_000817"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5523045.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO160_000818"
     CDS             complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO160_000818"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5523046.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO160_000819"
     CDS             complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO160_000819"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5523047.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO160_000820"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO160_000820"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5523048.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO160_000821"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO160_000821"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5523049.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43545..43652
                     /locus_tag="OO160_000822"
     CDS             43545..43652
                     /locus_tag="OO160_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523050.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43858..45141
                     /gene="gltA"
                     /locus_tag="OO160_000823"
     CDS             43858..45141
                     /gene="gltA"
                     /locus_tag="OO160_000823"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5523051.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45531..46097
                     /locus_tag="OO160_000824"
     CDS             45531..46097
                     /locus_tag="OO160_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5523052.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46148..48607
                     /locus_tag="OO160_000825"
     CDS             46148..48607
                     /locus_tag="OO160_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5523053.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48622..49353
                     /locus_tag="OO160_000826"
     CDS             48622..49353
                     /locus_tag="OO160_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5523054.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49350..50426
                     /locus_tag="OO160_000827"
                     /pseudo
     CDS             49350..50426
                     /locus_tag="OO160_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50578..51624
                     /locus_tag="OO160_000828"
     CDS             50578..51624
                     /locus_tag="OO160_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5523055.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51621..52412)
                     /gene="nei"
                     /locus_tag="OO160_000829"
     CDS             complement(51621..52412)
                     /gene="nei"
                     /locus_tag="OO160_000829"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5523056.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52448..53182)
                     /gene="pxpA"
                     /locus_tag="OO160_000830"
     CDS             complement(52448..53182)
                     /gene="pxpA"
                     /locus_tag="OO160_000830"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5523057.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53172..54104)
                     /gene="pxpC"
                     /locus_tag="OO160_000831"
                     /pseudo
     CDS             complement(53172..54104)
                     /gene="pxpC"
                     /locus_tag="OO160_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54098..54754)
                     /gene="pxpB"
                     /locus_tag="OO160_000832"
     CDS             complement(54098..54754)
                     /gene="pxpB"
                     /locus_tag="OO160_000832"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5523058.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54777..55520)
                     /gene="ybgI"
                     /locus_tag="OO160_000833"
     CDS             complement(54777..55520)
                     /gene="ybgI"
                     /locus_tag="OO160_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5523059.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55791..57272
                     /gene="dtpD"
                     /locus_tag="OO160_000834"
     CDS             55791..57272
                     /gene="dtpD"
                     /locus_tag="OO160_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5523060.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57422..58840)
                     /gene="phrB"
                     /locus_tag="OO160_000835"
     CDS             complement(57422..58840)
                     /gene="phrB"
                     /locus_tag="OO160_000835"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5523061.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58837..59346)
                     /locus_tag="OO160_000836"
     CDS             complement(58837..59346)
                     /locus_tag="OO160_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5523062.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59475..59762
                     /locus_tag="OO160_000837"
     CDS             59475..59762
                     /locus_tag="OO160_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5523063.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59817..60197)
                     /locus_tag="OO160_000838"
     CDS             complement(59817..60197)
                     /locus_tag="OO160_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523064.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60200..63586)
                     /locus_tag="OO160_000839"
     CDS             complement(60200..63586)
                     /locus_tag="OO160_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5523065.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63605..63808)
                     /locus_tag="OO160_000840"
                     /pseudo
     CDS             complement(63605..63808)
                     /locus_tag="OO160_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15692 a        17275 c        15617 g        15600 t
ORIGIN      
        1 agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc gactgttaat
       61 tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt tcggcctttg
      121 catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta tgagatggga
      181 ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa aaattcgcat
      241 ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac tggataactt
      301 gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca ggacaattgc
      361 agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg acccaccgcc
      421 atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag gcagtgattt
      481 cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc gtgacggacg
      541 agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat gccacttgta
      601 attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat gctcctttgc
      661 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
      721 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta
      781 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
      841 tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc cgcgctcgcg
      901 aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
      961 atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt
     1021 atcatgcaaa aaccgtgctg gaagcggacc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag ctgacagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa atgcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg caggatgact
    17821 cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca cgttttacta
    17881 ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    17941 aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt
    18001 tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat
    18061 ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact
    18121 tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat
    18181 cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac
    18241 ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg
    18301 gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt
    18361 agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt
    18421 tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg
    18481 caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg ccagcattcg
    18541 cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga tcgctgctgg
    18601 ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt
    18661 gccgctccac gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat
    18721 caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag
    18781 cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ctgacactac
    18841 tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac
    18901 tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac
    18961 gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt
    19021 aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg
    19081 cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt
    19141 gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca
    19201 gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt
    19261 tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc
    19321 cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg
    19381 cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt
    19441 tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac
    19501 agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg
    19561 aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag
    19621 attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga
    19681 acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag
    19741 ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct
    19801 tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga
    19861 ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta
    19921 ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac
    19981 aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat
    20041 gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc
    20101 gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg
    20161 tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg
    20221 taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca
    20281 acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca
    20341 tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga
    20401 gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa
    20461 ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag tgcggaccat
    20521 gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac
    20581 ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt
    20641 gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca
    20701 ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa
    20761 ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac
    20821 gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa
    20881 ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat
    20941 ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt
    21001 ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc
    21061 agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac
    21121 gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata gttattgata
    21181 tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc
    21241 cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata
    21301 tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgccgct
    21361 gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgcctta
    21421 tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc
    21481 ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt ggctttttca
    21541 gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct
    21601 ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc tgcggctttg
    21661 gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc tgcttcttcc
    21721 gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt ctgctttaac
    21781 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    21841 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    21901 gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga ttcctggctt
    21961 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    22021 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    22081 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    22141 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    22201 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    22261 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    22321 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    22381 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    22441 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    22501 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    22561 aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga tttcggactt
    22621 gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct
    22681 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    22741 cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc
    22801 ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    22861 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    22921 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    22981 ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac
    23041 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct
    23101 gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag
    23161 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cacgcgccgc
    23221 tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg aaaaaccaat
    23281 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    23341 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    23401 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg
    23461 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    23521 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    23581 acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc
    23641 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    23701 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    23761 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    23821 gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga gcggcccata
    23881 attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    23941 tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca
    24001 cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca
    24061 gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa
    24121 gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg
    24181 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag
    24241 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    24301 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    24361 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    24421 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    24481 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    24541 ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg
    24601 gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc cagtgccgga
    24661 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    24721 acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    24781 ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc
    24841 gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    24901 agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg
    24961 aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    25021 ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg
    25081 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    25141 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc
    25201 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg
    25261 cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata
    25321 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    25381 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    25441 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    25501 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    25561 aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    25621 accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc tgtcggcagc
    25681 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    25741 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca
    25801 atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag caggaagcca
    25861 cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg
    25921 gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    25981 agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgtctggtcg
    26041 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    26101 tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt
    26161 gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga
    26221 tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca
    26281 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    26341 acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta
    26401 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    26461 gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta
    26521 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    26581 gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa
    26641 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    26701 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    26761 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    26821 tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg
    26881 ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc
    26941 ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    27001 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    27061 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    27121 agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga
    27181 aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt acgccgcaaa
    27241 atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt
    27301 tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgatg
    27361 cccatttcca agacttatac attgataaat atcaatttct acaaattatg ttaatcatgt
    27421 ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata agactgaaat
    27481 tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac ggttgacctt
    27541 aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt aataaaggca
    27601 taaatctccc ccccgccgat attaacattt aattaactta taattatatt ataattaact
    27661 cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg agagtgaaga
    27721 taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
    27781 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
    27841 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
    27901 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
    27961 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
    28021 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
    28081 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat cccactgggg
    28141 gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg agcttcatcg
    28201 catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc gcctgctgag
    28261 ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga caagaaagca
    28321 gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc ctaggcctta
    28381 aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg gcaaaggttt
    28441 tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca gccatgccgt
    28501 tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc ggcgcttctt
    28561 tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc gttagcgaac
    28621 caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc aggacgcgaa
    28681 ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc ctgttgttat
    28741 gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt gcgctgcgtt
    28801 gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc tgccaggcct
    28861 catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac tcttcttttg
    28921 cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc aacacccggt
    28981 catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca ttgagcgcaa
    29041 tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca atcacgttgc
    29101 caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca atctggttga
    29161 tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt tgccgatcga
    29221 ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc tgaccgcgat
    29281 cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc acttcttccc
    29341 gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc ggcatgttgt
    29401 cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca gccagttcga
    29461 agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg ctgtcgtggg
    29521 catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg tatccaaaac
    29581 ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta ttttcaatac
    29641 gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat
    29701 atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct
    29761 tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca
    29821 gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca
    29881 gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt
    29941 tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca
    30001 cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac
    30061 cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga
    30121 tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct
    30181 cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag
    30241 aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta
    30301 ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg
    30361 ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga
    30421 tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc
    30481 gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct
    30541 ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg
    30601 ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc
    30661 aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc
    30721 agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga
    30781 gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc
    30841 ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc
    30901 tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt
    30961 gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa
    31021 tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat
    31081 ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg taagcgagtt
    31141 attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt
    31201 accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa
    31261 gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag
    31321 aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg
    31381 tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag
    31441 cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc
    31501 ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac
    31561 aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac
    31621 gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac gctcgagggt
    31681 aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcacagaaag
    31741 cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca
    31801 ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg
    31861 cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc
    31921 catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt
    31981 ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc
    32041 aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag
    32101 cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg ccagcaacac
    32161 cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct cgctgagtgt
    32221 ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc
    32281 ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc
    32341 agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc
    32401 acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcaggtt
    32461 catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac aaaacaatac
    32521 cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta ttgtttacag
    32581 gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca
    32641 caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct
    32701 taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg
    32761 ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca
    32821 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    32881 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga
    32941 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt
    33001 ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    33061 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    33121 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    33181 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    33241 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    33301 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    33361 aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta
    33421 gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg
    33481 cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga
    33541 atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt
    33601 tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta acatgctctt
    33661 tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg
    33721 cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta gagccaggga
    33781 tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc gtggtctgtt
    33841 taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta cccggtttgt
    33901 gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg cctgggcagt
    33961 tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc agcatatcca
    34021 gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg atagcttcca
    34081 gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa gcgccagtag
    34141 cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta gtgccgcctt
    34201 tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt tctgagtgga
    34261 aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta tcaattaaaa
    34321 tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt
    34381 tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt
    34441 accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg cgccgatgat
    34501 accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt
    34561 cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc
    34621 gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta tgtccatcgt
    34681 acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta
    34741 gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat
    34801 ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt tgccgtcgag
    34861 gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca gcgccaggtc
    34921 gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt
    34981 accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag
    35041 cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt tttcgatttc
    35101 cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt ctacaacggc
    35161 accgagatac agctctttag cgatatcggt cgccgcttca accagaatct ggttaaccgg
    35221 ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt tttctgcaaa
    35281 agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc
    35341 agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag cggcttcttc
    35401 tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa
    35461 cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt
    35521 tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt
    35581 aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt tgatcgttac
    35641 caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta
    35701 catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg
    35761 catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca gggaaccgaa
    35821 cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg tcagcttgcc
    35881 atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca tgccgagggt
    35941 atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg ccatgctgac
    36001 gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca cttccgggta
    36061 acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc
    36121 gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt
    36181 gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc
    36241 cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag ccagagccgg
    36301 ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt tcgccagatg
    36361 tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa tggcgctggc
    36421 gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg
    36481 ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc
    36541 gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt
    36601 taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac
    36661 ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc
    36721 gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg
    36781 gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca
    36841 ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg
    36901 gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc acggaaatga
    36961 tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc
    37021 ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt
    37081 tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg ttccagcagg
    37141 tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg
    37201 tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggaaacc
    37261 gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg acgcatcccg
    37321 cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg tacgcaaacc
    37381 tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc
    37441 ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat ccggccccat
    37501 ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca
    37561 ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata accatattca
    37621 aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc gccctgaccg
    37681 ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg
    37741 tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct
    37801 tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca
    37861 ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact
    37921 gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat ctcaactttg
    37981 ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg
    38041 ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc gcggtacagg
    38101 ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag cttgtcagcg
    38161 tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg ctgggttgcg
    38221 ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc gatgaagaca
    38281 tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc
    38341 ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat atcagtacag
    38401 tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg gttgttgata
    38461 acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagagtttcc
    38521 tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg cagcactttg
    38581 ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat aacaaccggg
    38641 cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg
    38701 gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc gaggtgttct
    38761 ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc
    38821 agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg
    38881 ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc ttccagcgag
    38941 aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc agcggcggtc
    39001 agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata
    39061 cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc ggcaccaatc
    39121 gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat ggtttctttg
    39181 ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt cagatcgtgg
    39241 aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg atctagattc
    39301 gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc
    39361 acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg
    39421 cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac
    39481 tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca
    39541 tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct
    39601 ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt
    39661 ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt
    39721 agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt
    39781 tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg gtaatgactt
    39841 gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc aacgcttgcg
    39901 cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt gatatggccg
    39961 atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga
    40021 cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca
    40081 cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg
    40141 ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac
    40201 agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt
    40261 tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg
    40321 tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg
    40381 cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg
    40441 ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga caggctggga
    40501 tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct
    40561 tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt
    40621 gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat
    40681 gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactcaggc
    40741 agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta
    40801 tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc
    40861 atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca
    40921 tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag ccctttcgcc
    40981 atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg cagcgcttta
    41041 cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag gcgatccaga
    41101 gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc gatagactct
    41161 tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga gttgccgccc
    41221 agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa cagtcccgga
    41281 acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac tttggtcgga
    41341 ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatcg
    41401 acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc gagaacttct
    41461 ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga
    41521 ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc caggtctttg
    41581 gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct
    41641 tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt cgggtggaat
    41701 tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc gacaccgtcg
    41761 ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc gccagtcgcc
    41821 agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca cagtgcggta
    41881 caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa
    41941 atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc gtgaccggta
    42001 cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg
    42061 ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag gcccatgtgt
    42121 tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca
    42181 ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg
    42241 gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg
    42301 aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc
    42361 atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg caatttcatc
    42421 acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca acgacaatca
    42481 ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac acctgccaca
    42541 tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac actttggtga
    42601 acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg gtggcgaaaa
    42661 aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga
    42721 aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc
    42781 gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt tacccgcttc
    42841 gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg
    42901 atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat
    42961 cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa gccactgcag
    43021 gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc
    43081 cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt
    43141 tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact acgggcacag
    43201 aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt
    43261 cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac
    43321 gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt
    43381 ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg
    43441 aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa
    43501 tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt aaaagttatg
    43561 aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat
    43621 tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg
    43681 ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct gtacccaggt
    43741 tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg
    43801 cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg
    43861 gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact ggatgtgctg
    43921 aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtgttc
    43981 acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat
    44041 ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct
    44101 aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat
    44161 gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg
    44221 ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat taccggcgcg
    44281 ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc
    44341 gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt
    44401 ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt cctgaatatg
    44461 atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac
    44521 cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc
    44581 gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg
    44641 ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aattagctcc
    44701 gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg
    44761 atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa
    44821 acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga agtggctatg
    44881 gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact gtacccgaac
    44941 gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc catgttcacc
    45001 gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga aatgcacagt
    45061 tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt
    45121 aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta acccgccgtt
    45181 caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat aaaatccact
    45241 atcggacatt catatattct aaaaaaaata catcggtata aattataaac aaatagatga
    45301 tttttaatca tctatcttcc tctcagttac atgaataaac caccaaatat agcgcacccc
    45361 tataattccc aatactttat tcatcaaaca tttaattcat accatcaatt aattactttt
    45421 acatttaata aaattaataa tatttttaag aaaaactaaa aacattcgac aatcaaataa
    45481 gatattcttg caccgatata cttttttctt ttctgttaaa ggactcagtt atgtttaaag
    45541 gacaaaaaac attggccgca ctggccgtat ctctgctgtt cacagcacct gtttatgcgg
    45601 ctgatgaagg atccggtgaa attcacttta aaggtgaagt tattgaagca ccgtgtgaaa
    45661 tacatcagga tgatattgat aaagaggttg aactcggtca ggtgaccacc agccacatta
    45721 atcagtcaca tcacagcgat gccgttgctg tcgacctgct cttagtcaac tgtgatctgg
    45781 aaaactccag caacggttcc ggtggcaaga tttctaaagt tgcagtcacc tttgatagct
    45841 cagcgaaaac caccggcgca gatccgattc tcaacaacac cagcacgggt gaagccaccg
    45901 gcgtcggcgt acgtttaatg aataaagatc aaagcaatat cgttttaggc accgctactc
    45961 cagatatcga cctggctccg acctccagcg aacaaacgct gaatttcttt gcctggatgg
    46021 aacaaattga tcaggctaca cctgtaacgc caggcgcagt tacggcgaat gcaacctacg
    46081 tgctggatta taaataagta ttactttggc ggcgctatcg ccgcctttca taaaatattt
    46141 atcaggtatg gacaccgtga atatttatcg actctctttt gtatcctgcc tggtcgtggc
    46201 gatgccttgc gcattggcgg tcgaattcaa tctgaatgtt ctcgataaat caatgcgcga
    46261 ccgcattgat atttcattat taaaggaaaa aggagtcatt gctcccggtg agtattttgt
    46321 tagcgttgcg gtaaataata accaaatcag taacgagcaa aagattaact ggcacaaaaa
    46381 tgacgataaa accattccgt gcatcaatga tttactggtc gataaatttg gcttaaaacc
    46441 tgaagtccgt cagtcgttac cattgataaa tcagtgcgtc gattttagct cccgacctga
    46501 aatgctcttc aatttcgatc aagccaatca gcaactgaat attagtattc cgcaagcctg
    46561 gctggcgtgg cactcagaaa actggacccc accctccaca tggaaagaag gtgtcgccgg
    46621 tgtcctgatg gattacaact tgtttgccag cagctaccgc ccacaggatg gcagcagcag
    46681 cactaatctt aacgcctatg gaacagccgg aatcaacacg ggggcatggc gcttacgcag
    46741 tgattatcag ttgaatcata ctgatagcga tgataaccat gaacagtcag gcgaaatatc
    46801 acgtacctat ctttttcgcc cgttaccgca attaggttca aaattaaccc tcggcgaaac
    46861 ggattttagt cccaatattt tcgacggttt ttcttatacc ggcgcggcac tggcaagtga
    46921 cgatcgaatg ttgccatggg aactacgcgg ctacgcccca caaattagcg gtattgcaca
    46981 gaccaatgcc acggtgacga tcagtcaatc aggccgcgtc atttaccaga aaaaagtccc
    47041 accaggccca tttatcattg acgaccttaa tcagtctgtt cagggcacac tggatgtcaa
    47101 agtgacggaa gaagatggtc gggtgaacaa tttccaggtt tcagcagcat cgacgccctt
    47161 cctgactcgt cagggacagg ttcgctataa actggccgcg ggtcagccac ggccctccat
    47221 gtcacatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg ggatgctgtc
    47281 aaacacctcg ctgtacagcg gcctgctgct ttctggtgat gactaccatt ctgccgcaat
    47341 gggtattggg caaaatatgc tgtggcttgg tgcgctgtcg tttgatgtca cgtgggccag
    47401 tagccatttt gatactcagc aggacgagcg gggcttaagc taccgtttta attacagcaa
    47461 acaagtggat gccactaaca gcacgatttc cctcgccgct tatcgtttct ccgatcgtca
    47521 ttttcacagc tacgccaact atctggatca caaatacaac gacagcgatg cgcaggacga
    47581 aaaacagacg atcagcttat ctgtgggcca accgattacc ccactaaacc tcaatcttta
    47641 cgccaacctg ctacatcaaa cctggtggaa tgcagacgcc tccacgaccg ccaacatcac
    47701 ggcaggtttt aatgttgata ttggtgactg gagagatatc tctatttcta cgtcattcaa
    47761 tacaacccat tacgaagata aagatcgcga caaccagatt tacctgtcga tttcgctccc
    47821 cttcggtaac ggtggtcggg ttggctatga catgcaaaac agtagccaca gcaccacaca
    47881 ccgcatgtcg tggaacgata cgctggatga acgtaatagc tggggcatgt ctgccggact
    47941 gcaatccgac cgtcctgaca atggagccca ggtgagcggt aactatcagc acctgagttc
    48001 agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt ccgtcagcag
    48061 cagctggagc ggttctttca ccgcaaccca atatggtgca gcgtttcatc gccgcagctc
    48121 caccaatgaa ccacgcctga tggtcagcac cgatggcgtg gcagatattc cggttcaggg
    48181 caatctcgac tacaccaacc attttggcat tgcggtggtg ccgttgattt ccagttacca
    48241 gccttccacc gtggcggtga acatgaatga cttacccgac ggcgtaacag ttgcagaaaa
    48301 cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcactgg cttcccgttc
    48361 cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagtttcctc ccctcggagc
    48421 ggatatccgc caggatgaca gcggcattag cgtggggatg gttggcgagg aaggacatgc
    48481 ctggttaagc ggagtcgctg aaaatcaaaa gtttaccgtg gtctggggtg atagccagca
    48541 ttgctcgctc catcttcctg aacatatgga agacaccgca aatcgcctga ttttaccttg
    48601 tcattaatta aggaaatgaa aatgacattt atgaaaggac tacccctttt gttgttggtt
    48661 gccagtctgt gcagccacgc tgcactacaa cccgatcgca ctcgtattgt gtttaacgct
    48721 aatgataaag ctaccagtct gcgggtggat aatcgtagcg ataaactccc ctacctcgct
    48781 tattcctggc tcgaaaatga aaaaggggag aaaagcgatg atcttctggt cgccctgccg
    48841 ccgatacaac gtctggaacc gaaagcaaca acacaggtac ggatagtaaa gcaagcctcg
    48901 accacaaaat taccgggcga tcgcgaaacg ctatttttct acaatatgcg tgaaattcct
    48961 cccgccccgg aaaaaaacag cgaccatgcc gttctgcaag tcgccattca gagtcgtatc
    49021 aaggtgttct ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt cgaactgcag
    49081 ttacaggtca gccagcaggg caaccaactg acactgaaaa atcctaccgc gtattatctg
    49141 accattgcct acctgggacg aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg
    49201 gttgcgcctt ttagtacggt caacacaaac accggaaatt atagcggcag ccagttttat
    49261 ctcggttata tggatgatta cggcgcgtta cgcatgacga cgctcaactg cagcggacaa
    49321 tgccatttac aagccgtgga ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca
    49381 tctgtctgtt atttctgtcg ttgaagtctg cgcttgccct gaactgctac tttggttcat
    49441 ccggtggctc ggtagaaaaa tcagaagcga ttcaaccttt tgctgtacca ggcaatgcca
    49501 aactcggcga taagatctgg gaatctgacg atattaaaat aagatctggg aatctgacga
    49561 tattaaaatt cccgtctatt gtgacaacaa taccaacggc aactttgaaa gcgaacatgt
    49621 ctacgtatgg gtaaacccct acccgggggt gcaggatcgt tattatcaac tcggagtaac
    49681 ttacaatggc gttgattatg acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg
    49741 tatcgacagt aagaacattg acatttatac ccctgatggg atggcagaac aaaatttgct
    49801 cgggtgatcc cgctaatatt cacatgtcgc gaacctttct cgctcgcatg cggttatacg
    49861 tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg
    49921 tgcaatttga cggtgccggt agcgttaacg aagaccctac tgcccaaaac ctgaaatatc
    49981 atattactgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt tccatttcac
    50041 cggaaacaca gatgattgat tttggtaaat ttaatttgct ggatattcgt cgccacacaa
    50101 tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc accgacggat
    50161 ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat aaagcattgt
    50221 tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct tataccttca
    50281 ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa aaaacctata
    50341 cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc gatacagtgg
    50401 tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc ggaaagggca
    50461 tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt ttttcctagc
    50521 ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga gcgaaggatg
    50581 ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat tggtttcctc
    50641 gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat catcttcagt
    50701 atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca ggccattctt
    50761 ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc cgccaactgg
    50821 ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt gggttggtta
    50881 ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc tccaggcggc
    50941 gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct ggtggcgttt
    51001 atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg
    51061 ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc agtgagcatt
    51121 aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg catgctgcgt
    51181 cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca gtctggtcag
    51241 ctcatcacca tcgaactacc ggaatggctg ctggcgatgg cgtatatggc aattggctgg
    51301 cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc gctaccgcaa
    51361 atcctgctgt cgatttttgc tctgctggct atttgtgcag gtatggcgtg gggactgacc
    51421 cggtttatgc atattgattt tatgactgcc tacctcgcca ccagtcccgg cgggcttgat
    51481 accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat ggcgatgcaa
    51541 accctgcgcc tgttcagtat tttgctgacg gggccagcca ttgcgcggtt tatttcaacc
    51601 tatgcgccga agcggtcggc ctagtgctgg cagcctggac accagtaaaa cgggcgcgag
    51661 gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc acagttcgcc atcacgatga
    51721 aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg cccccgcgta
    51781 gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga gtgcatccag ttgcgccgca
    51841 ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat ctccacccgc
    51901 aaataattgc caagcccagc cagaaacgcc tgatcgagca gtaatcctgc aaactgacgg
    51961 ttacgaaagc gcggcgacaa taaccgttcc tttaccacct ccggcgtcag attcggatcc
    52021 agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc cggcctcaac
    52081 atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg cagttttacc
    52141 cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca gacgccgtaa
    52201 agctgattat ggctgtagag cgttaaaccg ttgggaaaat gagttaacaa cgccttacca
    52261 cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt ttttaactgc
    52321 gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc caggttatcc
    52381 gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca ttctttatta
    52441 agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt aatcggcggg
    52501 cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc gcgaccgttg
    52561 cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc gtttgcgcca
    52621 acgcctgttc ttcgttttca atcaacgcgc ctggctgact tcgcggcacc agcgagccgt
    52681 cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc
    52741 ctgcacgaat cagctcgctt cctgccagcc caacgagaac caatgccgga tcgcaagcgt
    52801 ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg ttgtacaaca
    52861 tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc gttgccagcg
    52921 cgccaatttg atacagcgtc tgggcgtaaa cggtttccgg cggcagctgc atggcgctgc
    52981 gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aatcgcgaca ccatttttta
    53041 ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat ccacaagcaa
    53101 tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct tcgcccagat
    53161 cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa ataacgttgc
    53221 tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg aatcggctga
    53281 ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatacaggc aatacgtgga
    53341 taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt atgcggcacc
    53401 tgcaccacgc ccggtaacaa accgtgagat aacagttcgc gatcggtgat gcgttttaaa
    53461 atttgcccct gtaaacgata gcccattcgg ttactttgcg agctaagctg ccagggcgaa
    53521 cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc cggcaaggcg
    53581 cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa atcacgcttc
    53641 gatttgccaa tcgggagtca gtcaccatcc ttcagtaaac ggccttccag cccgccaatc
    53701 cccactttga gatcggtgct gcatgacccc attaccggcg gaacatcaat accacccgcg
    53761 accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct
    53821 ttcatcggca atcgccagcc ggtccagacg gcgttatcat ccaaccgcgc ttcgcaacct
    53881 gcacccgtca gggcaaacca cccatcagtt tcgaactcaa ctgttaactg accgagcgtg
    53941 atctccagcg cgggggcatt ggcgtcatta cccaccagca ggttagcaat gcgcagcgca
    54001 ggcatatcca gtgcgccaca gtggctgata cccgactggc aaaaaccgtg acgaccgcca
    54061 tcctgcacag tggtgtacat gcccgcacga ataatcttca gcatattccc tccttctgcg
    54121 gtacaaatcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc gccggatcaa
    54181 acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc agcggataaa
    54241 caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagcaag cgcggttcag
    54301 cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag cccggttgaa
    54361 aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc
    54421 tcaacccgca atacgccgcg accaccgcca aatccggtcc gcctgcacca ccgtaaacca
    54481 ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc caccagcgtt
    54541 gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc accgtgatat
    54601 tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc tgcgccagtc
    54661 gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc actaccgccg
    54721 tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat tattcgttat
    54781 gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt cagccactcg
    54841 ctcaatgcgc gaataccacc gcgttcagtg gcatggtgac ctgcagcata aaaatgcaat
    54901 ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat aaaagcatcc
    54961 acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca ccaggcgacg
    55021 cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc cagacgcgct
    55081 tcaatccagg aggctagttc cattcccggc acaggcatgg tcagctctcc ccacggcacc
    55141 aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg tgcgttattg
    55201 cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat atcattcgcc
    55261 agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc tcctttccag
    55321 aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc atcgagcaaa
    55381 gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc
    55441 aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt aatcagttgt
    55501 tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca ccgttaacat
    55561 agcgttacat cgcctgttcg tcgatagcca atcttttgac atattaagaa acaaacccga
    55621 cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat ttatgcaatc
    55681 agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta aggaaacaca
    55741 agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt atgaataaac
    55801 acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg gaatatttta
    55861 gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta aaatacaacg
    55921 atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc acgccaatcc
    55981 tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt
    56041 tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg tcattcctct
    56101 atctgtccct ggcgattatc gtctgcggct atggcctgtt taaatctaac gtaagctgtc
    56161 tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt
    56221 atgcggcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac gcccaggaag
    56281 agtacagctg ggcgatgggc tttggcctgg cggcggttgg aatgatcgcg ggtctggtca
    56341 ttttcttatg tggcaatcgt catttcactc atacccgcgg cgttaacaaa aaagtactgc
    56401 gtgcgacaaa ctttctcctg ccgaactggg gatggctgct ggttctgctg gtggcaacgc
    56461 ctgcactgat taccgtgctg ttctggaaag agtggtcggt atacgcctta attgtcgcga
    56521 ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac cagaaacagc
    56581 ggaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc tgggccttcg
    56641 cacaacaggg cggtagctcg attagccttt atatcgaccg cttcgttaac cgcgatatgt
    56701 ttggttatac cgttccgacc gcgatgttcc agtcgattaa tgccttcgca gttatgctgt
    56761 gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt accgtgcgca
    56821 tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc attctgacct
    56881 taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg gtgttaggcc
    56941 tcgcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg caaattacgc
    57001 gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg ctttctggcg
    57061 cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct tcgtttgatg
    57121 cttccggggc gatcaactac tccatcaatg catatattga agtatttgat caaattacct
    57181 ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag gcgctgaaat
    57241 tcagaaaccg cgcgctggcg ctggagtcat aataggccag caccgatgcc tgatgcgacg
    57301 cttgacgcgt cttatcaggc ctacgtattt cctgcaattt attgaatttg cacaaatttg
    57361 taggctggat taggcgttca cgccgcatcc ggcatctggg ctcgattgcc tgacgcgtct
    57421 gttatttccc cttccgcgcc gcctcatacg ctgccaacgt ttgtactctc gcctctttgt
    57481 gctcgactat cggttgcgga taatccagct tcacacctgc tttctgcgcc cacttccacg
    57541 gctcatgcac cgctttccct ggtacatcgc gcagctccgg taaccatcga cgaataaact
    57601 cgccctcacg gtcaaatttc tcgccctgag ttatcggatt gaaaatacga aaatacggcg
    57661 cagcatcagt acccgtagaa gccgcccact gccagccacc gttattagct gccaaatcac
    57721 catcaatcag ctgcgacatg aaatatcgct cgccttcgcg ccagtcgatc aacaaatctt
    57781 tcaccagaaa actggctgta atcatccgta gccggttatg catccagcca gtgctgttaa
    57841 gctgacgcat agcggcatca ataatcgggt atcccgtttt gcctttctgc caggcctgta
    57901 aatgtgcggg attgctttgc cactgcacac gatccgtcca ggcaataaat ggacgatgtt
    57961 tacacaacga ggggtaatac gtcatcagat gacggtagaa ctcgcgccag atcagctcac
    58021 taagccagac actaccggcc ccaccgtcca gcgcctgcgg ctgttccgcc aacaagcgat
    58081 gcaaacactg gcgaggcgat aacccgcccg ttgccaagct ggcggacaaa cggctggtgc
    58141 cttccactgc cggaaaatct cgttgttgct catactctcc agcaccgttc tcgcaaaact
    58201 ggcgtaattg cgcaatcgcc gctttttctt ccaccgggaa atgcgcagta tcgaaagact
    58261 gacgaggata attcagcgta atggatggcg cgggctttat cgatccgcta ctacgaactt
    58321 ttggtgcagc gacgcactcc ggcatccctt cccgcagccg tttcagccag gcattcttaa
    58381 aaggcgtaaa gactttgtac atctcgtgat tgccggtcat caccgccccc ggcggcagga
    58441 tcacgctgtc atcaaatcct tcacacacca cgttacgcag cgctctttca acgtccacat
    58501 cccgcgctcg ctcattcacc tcatactgat agttataaaa aagatgagta acgctgtttt
    58561 ccgcgcacac ctgtttaact atttcgacac tggcgacaaa gtcatccact tcacggaaca
    58621 ataaaggaat acctttttcc gccagcgcga tttgtagccc attcagttga gcattgataa
    58681 gttcagcctg acgcggcgac atgttatgcg tctcccactt gcgtggtgta gcgatataca
    58741 gcgccagcac gcgtgcagac gaattgcggc aggcggcagc cagtgcgaga ttatcgtgca
    58801 gacgtaaatc ctggcgaaac caggccagat gggtagtcat aaaactcctg aatggcgcaa
    58861 gttaatcaca cgcggccaaa gttcgaaata acgctgccct gaaagccagg cgtcaggata
    58921 aagcgccata tagtgtttga tacgcatcag cggcgcaaga agtggttgct cgccacggcg
    58981 ataactgtcg ataagcgcag ccagctgctg gcgttctgtg gaatcaatat gcgggcgaaa
    59041 ataaccctga acgtgcatca aaacgttggt gtgatcgcgc gggttggcgg gatgagaaag
    59101 caaaacaatt acgcgttggc ggtactggtt gtaaaaatca tccagatttt gccactgatg
    59161 aatatcagcc acaaacgggc caagtttgcg gtactcgggc tgagaatggg ccagaaagac
    59221 gagtttatag cggctgtgat aatcgagaag cgccccgcgc gttaagccat gttctttcag
    59281 tcgatgtaac tcatgaaggg cgaagatacg gattagggtg ctgtcatcaa atcgttgtag
    59341 attcattact gctcctgaac ggtatacagg agtaagtgta ggcgttggta aatgatgttt
    59401 gtgaagttaa tccgctagca gggcaagatt acgaaagcgc gctccccgca agcaactaat
    59461 caaggtagtt aatcatggct gaacacgata ttacgccaga agtcactatt tccaaaacac
    59521 agcgccgcta taaagtcggc tatgtcagag cccgccacca ggaccgcaaa accggtatag
    59581 cccggtatta ttcacaacat cccagtctgc accttaaggg cgactggctg aaagaggccg
    59641 gatttgatac cggacggggt gtcacggtga agatttcgga ggggggcctg accatcatcg
    59701 ctgacagtga cgaggtgcag gaactgcggg atgggatgtt tagtgtgctg aacgaaagtt
    59761 aaatattaaa aaagctggga agatttaaaa ccttcctagc tttttattct gtcttattat
    59821 aatggatact catcatcttc cgatgaattt ataaattgct ccaagtttac atgaagagta
    59881 cttaacctag tttttaactc attaagtgaa gcgataattt catattttcc aacataatca
    59941 tatgatggat cgtattcatt atctatgtca gaagctaaaa atgatgatat tcgtagttta
    60001 aatttagctt catctttatt acattcattg gcataaggtg aatgccctat catagcatat
    60061 tcatctaatc taaaaggata agatggtttt aattcatcga tcaatgaaat ttcaatccat
    60121 gttctatatc caggcattaa tggactaatt tctttgagca aaacctttcc acttaataat
    60181 gtattgagat tgttcattat tatgtctccc acgggaaaaa tatatgtcta acacttccac
    60241 catgttgatg aacttgtaaa tgtggtaaat caccatcagg atttcctggt ccatgacctg
    60301 acactctata tgaaggtttt gttacagaag gatcttttat atcatcccaa tgatatgaac
    60361 ctgcttttgt tttattttca gtaagatatt ccattaagtt cttagtgctg gggcctgact
    60421 cacctgtcat atttttataa ttatgaccta aatatctatc cataaacaat tgttcagcgt
    60481 cacttctaga cctaacagta actttggttt cactactatc taataaacga ttcgcttttt
    60541 ggttactaac agcacatgga gttaatccaa gtggatcagc atattttaga ggattaggtg
    60601 cataactgta gagatttaaa ccgcctctca accctatcgg gtcagttacc atgaaccgcc
    60661 cgacatccgg atcatagtac ctgtgcagat tgtagtgcag ccccgtttcc ctgtcgaggt
    60721 actgtccctg gtagcgcagg ttctgctcca ccccgccgtg gtccttctcc tgtatcgtgt
    60781 taccccagac ctgatacccc gtctcccaca cgattttccc gtcgctgtcc gtcatctctt
    60841 ccggcgcgcc gttcacatcc gtgtgaaaat acagtatccg gttcgggccc tctttgcccg
    60901 ccttatccac ccgcgccagc ggttcataac tcccctggtc cctgtagatg tacaggctgc
    60961 ctgttccctc cggcctgctc tcctgcgcca gccgcattcc gtcccataaa aattttgtcc
    61021 ggttgtacgg cttaccgtca gggctgatgt gctgtttttc cgtccgcctc cccagtgcat
    61081 cgtacacata ccggtatgtc ccccgcgctg tcgtcacttc caccatccgg tgttccgcat
    61141 cataacggta actctgcgtt ccgctccggc cctccttttc caccgcacgg ccaaattcgt
    61201 cataacggta gtgcacacca cggtaagaca tcagccggtt gaacggcacc acgctgtttt
    61261 ctgccgtggc cctttccccc gcgcgcctcc ccagcaggtt tcccgccgcg tccacctccg
    61321 tttccggctg gtgccacacc gggtgccact gcgcctgctc cacccgcccc agcgggtcgt
    61381 gcgtctccgt gatatgcccc tgcaggtcat acacatagcg ccatttgccg ccctgagggt
    61441 cggtcatccc cagcaacagt cgttcttcgt cgctccagcg gaatgtcgtc acctgtccgg
    61501 cttcgtcctg gtacgccgtc acctgattct gcgcatccca ctggtgataa cgtgtgcgcc
    61561 ccagtccgtc cgtcacgcac agcctgcgct gcgccaggtc ccacgccagc cggtactgtt
    61621 caccgctgtc cgtgtggtgc cccgtcaccc gccatgccgc gtcatccgga ccttcatacc
    61681 agccccactg gtaataatac ccttcgccgc ccggctggcg gtgcagcgtc atgcagtggt
    61741 tttcgtcgta agcaaactca cgcagcaggt gccccgtgct gtcgtacacg ccgttcaggt
    61801 ggccggcctc gtcatagctg taacgacgca gcagtaaagg tgcagtctca gggaaatgct
    61861 gataaatctg tgtcacccgc cggggatgcg ccgccagttc gtaatacaga cggatgcacg
    61921 gacgctgctg ttcgccgctg atttccacaa tgcggccccg gtcgtcgtag tacagattca
    61981 gacagttgct gttacggtcg cccagcattt tcagccgctg gcgctgcggg tggtgcgggt
    62041 cagcctcaaa cagaaaacac tgcccgtcat cgctgctgat aagccagtgc ccctgctcac
    62101 cacgccttac cgccagccct tcaccggggc tgtaaaaccc gccccccggc agtacggctt
    62161 ccatatccaa gcgtcgcccc atgcccgaca caaaggtcat gcagttttcg tctgcgctac
    62221 ccggtgtgca cacgaggctg acttcataca ttacactcca gccctgacca aacattcctt
    62281 ccgtacgttc gtcacgactg ctgtacacgc gctgccagtc cagcggaaag tggcccggca
    62341 gactgaaatc gaagtcatcg tcatcggcca ggtatttggc cccggtggcg gcattgaccg
    62401 gatgcggaag ggcactggtg gccttctgcg tcagcatgct catcccgaca ctggcagcaa
    62461 aacaggcgat tttagaaagc attttgcccg ggcgaagaaa catcagcgcc acggttgcca
    62521 ccagggttat ctgcgacctg ccgcttctga tatccctgac caccagtaac ggaccgccaa
    62581 tgcgcacatc cggtgacaca aacgccccgt tccccggctc attcaccacc ctggcctcgc
    62641 aggtacagcg tgccccgctg cgtgtcgccg gctggctgtt gatacgcaca tcccgcgcgc
    62701 cttccgcgat atattccgct gcgggcggct cgtcatcgcc tttgaacaga ttactgatgc
    62761 tgccaataac cgcattcact ccctgaaagg cggtttccag cataccgccc ggattcagca
    62821 ggttaagggc cgttcccagg gggtcatcca gaaaggcggc ttttttttgt gcgagaaact
    62881 gcgtcagtga ggaaggatgt ttatggcagt cgattttatc gtcaggggtc ggggtggatc
    62941 ccggggccgc actggccaca gtgggctgcc atagttccga cacaaaatgt gtcacgccat
    63001 ccaccatttc agaacgccct tcttccgtac tgagctggtc gtgcagagcc cgggacatca
    63061 gcacaggccc tgtcaccggg ttaaccgcca gggctatggc ccccgccatt tccagatatt
    63121 ccccggccac atcccggaac gttctggcat cctcctgacg ctgcgcttct tcctgttctg
    63181 ccttcgcctg cgcttcctgc gcctctatgt cctgccgcga tgccgcagtc gccgctgccc
    63241 gcgcggcagg tttgctgttg atccatgtgt caccagagcc ggtgaggatg taaccttcaa
    63301 tctgaggcgg gaaaagcata tttgcgaggg ctgaacaggc ttcgctgacc ttttctgtca
    63361 cgccggtgta caccatcgcc acacccgcca caaaaccggt cagcacgacg gccgtgcacc
    63421 ccatcgtgat ccccactgac gtcgccagta acgaggttgt gccgacaatg agggcatcaa
    63481 taacgacatt tgcggccacc tccagcaccg cccccagcac atctgccata aacgacgtat
    63541 gcagcagggc atcaccctgt cttgccgccc agaatgcctc ttccatgtcc cgttccctgt
    63601 atgattaatt cgtcctgact tcctgccgca tcctgaagcg cagattcttt ttaatttcag
    63661 cccagcgcgt catatctgcc ggggtcagcg gtgtggttct ggcgtaggtg aaggccatca
    63721 tgacccgctc acccggcaat acaaatgcca cctgacactg gtacacctgc tcgttacccc
    63781 ggagaaactg gctctgtatt tccaaaccgc ctcgttgaga taatattccg tcataatgta
    63841 aatagtccgt tttaataaag gaattattta ttgacaggtt ctgttttttt aatcatgaat
    63901 gtaataccca tcaccgcttt acagcacact ttatctttcc agtcataatc actaataata
    63961 ttaagcaatt agcaatattt acaacgacca gagcggatac atagttatgt atttaacaat
    64021 cccaatatat tcactatgca aataaaacca ctagtaacag acacgctacc gcctccatat
    64081 aaaaatggac accttataaa attgtatctt gcatagaaaa agctgaaata tcggatgcgt
    64141 tttaattatc aactataaat ctataagctt tttattaaca acaa
//
LOCUS       DAJTNP010000012        60420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTNP010000012 DAJTNP010000000
VERSION     DAJTNP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..323
                     /locus_tag="OO160_000841"
     CDS             <1..323
                     /locus_tag="OO160_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5523066.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            436..1230
                     /gene="panB"
                     /locus_tag="OO160_000842"
     CDS             436..1230
                     /gene="panB"
                     /locus_tag="OO160_000842"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5523067.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1242..2093
                     /gene="panC"
                     /locus_tag="OO160_000843"
     CDS             1242..2093
                     /gene="panC"
                     /locus_tag="OO160_000843"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5523068.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO160_000844"
     CDS             complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO160_000844"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5523069.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3343..3723
                     /gene="panD"
                     /locus_tag="OO160_000845"
     CDS             3343..3723
                     /gene="panD"
                     /locus_tag="OO160_000845"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5523070.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3727..4956)
                     /locus_tag="OO160_000846"
     CDS             complement(3727..4956)
                     /locus_tag="OO160_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5523071.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5020..5460)
                     /locus_tag="OO160_000847"
     CDS             complement(5020..5460)
                     /locus_tag="OO160_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5523072.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5565..6335)
                     /locus_tag="OO160_000848"
     CDS             complement(5565..6335)
                     /locus_tag="OO160_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5523073.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6332..7258)
                     /locus_tag="OO160_000849"
     CDS             complement(6332..7258)
                     /locus_tag="OO160_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5523074.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7367..8029
                     /gene="can"
                     /locus_tag="OO160_000850"
     CDS             7367..8029
                     /gene="can"
                     /locus_tag="OO160_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5523075.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO160_000851"
     CDS             complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO160_000851"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5523076.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8812..11202
                     /gene="gcd"
                     /locus_tag="OO160_000852"
     CDS             8812..11202
                     /gene="gcd"
                     /locus_tag="OO160_000852"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5523077.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO160_000853"
     CDS             complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO160_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5523078.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12965..13312
                     /locus_tag="OO160_000854"
     CDS             12965..13312
                     /locus_tag="OO160_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5523079.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13418..14284
                     /gene="speE"
                     /locus_tag="OO160_000855"
     CDS             13418..14284
                     /gene="speE"
                     /locus_tag="OO160_000855"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5523080.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14300..15094
                     /gene="speD"
                     /locus_tag="OO160_000856"
     CDS             14300..15094
                     /gene="speD"
                     /locus_tag="OO160_000856"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5523081.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKARYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15132..15494)
                     /locus_tag="OO160_000857"
     CDS             complement(15132..15494)
                     /locus_tag="OO160_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5523082.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO160_000858"
     CDS             complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO160_000858"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5523083.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18621..20474
                     /locus_tag="OO160_000859"
                     /pseudo
     CDS             18621..20474
                     /locus_tag="OO160_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO160_000860"
     CDS             complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO160_000860"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5523084.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO160_000861"
     CDS             complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO160_000861"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5523085.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO160_000862"
     CDS             complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO160_000862"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5523086.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO160_000863"
     CDS             complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO160_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5523087.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTSIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28318..29691
                     /gene="aroP"
                     /locus_tag="OO160_000864"
     CDS             28318..29691
                     /gene="aroP"
                     /locus_tag="OO160_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5523088.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO160_000865"
     CDS             complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO160_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5523089.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO160_000866"
     CDS             complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO160_000866"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5523090.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31224..32117
                     /gene="nadC"
                     /locus_tag="OO160_000867"
     CDS             31224..32117
                     /gene="nadC"
                     /locus_tag="OO160_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5523091.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32320..32760
                     /gene="ppdD"
                     /locus_tag="OO160_000868"
     CDS             32320..32760
                     /gene="ppdD"
                     /locus_tag="OO160_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5523092.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32770..33917
                     /gene="tadA"
                     /locus_tag="OO160_000869"
                     /pseudo
     CDS             32770..33917
                     /gene="tadA"
                     /locus_tag="OO160_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33907..35109
                     /gene="hofC"
                     /locus_tag="OO160_000870"
     CDS             33907..35109
                     /gene="hofC"
                     /locus_tag="OO160_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5523093.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO160_000871"
     CDS             complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO160_000871"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5523094.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36349..36387)
                     /locus_tag="OO160_000872"
     CDS             complement(36349..36387)
                     /locus_tag="OO160_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523095.1"
                     /translation="MIIKTLKMSARK"
     gene            36412..37032
                     /gene="coaE"
                     /locus_tag="OO160_000873"
     CDS             36412..37032
                     /gene="coaE"
                     /locus_tag="OO160_000873"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5523096.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37032..37775
                     /gene="zapD"
                     /locus_tag="OO160_000874"
     CDS             37032..37775
                     /gene="zapD"
                     /locus_tag="OO160_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5523097.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37785..37982
                     /gene="yacG"
                     /locus_tag="OO160_000875"
     CDS             37785..37982
                     /gene="yacG"
                     /locus_tag="OO160_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5523098.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO160_000876"
     CDS             complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO160_000876"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5523099.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO160_000877"
     CDS             complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO160_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5523100.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO160_000878"
     CDS             complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO160_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5523101.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO160_000879"
     CDS             complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO160_000879"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5523102.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO160_000880"
     CDS             complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO160_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5523103.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO160_000881"
     CDS             complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO160_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5523104.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO160_000882"
     CDS             complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO160_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5523105.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO160_000883"
     CDS             complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO160_000883"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5523106.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO160_000884"
     CDS             complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO160_000884"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5523107.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO160_000885"
     CDS             complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO160_000885"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5523108.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO160_000886"
     CDS             complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO160_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5523109.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO160_000887"
     CDS             complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO160_000887"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5523110.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO160_000888"
     CDS             complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO160_000888"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5523111.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO160_000889"
     CDS             complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO160_000889"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5523112.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO160_000890"
     CDS             complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO160_000890"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5523113.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO160_000891"
     CDS             complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO160_000891"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5523114.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO160_000892"
     CDS             complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO160_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5523115.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO160_000893"
     CDS             complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO160_000893"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5523116.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO160_000894"
     CDS             complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO160_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5523117.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16733 c        14850 g        14365 t
ORIGIN      
        1 caaaggttgg tactgaaaac gggcaactcc ttggtaatac tctcacaggt aatgatgcag
       61 caaaaggagt cggtgtactc atagaaggtt tagcaactag taaaaatcct ctaatgacat
      121 tgaaacctaa tgattcaaat tctgtttata aagattacga cccgagaggc aaagacgata
      181 caacaggagg agtttacccg gatcaagata caggtataac ataccctctc catttccagg
      241 ccacgctaca acaggatgga actataccaa tagaagctgg tgagtttaaa gccaccagta
      301 ctttccaggt aacctaccct taataagtcc accgcaccgc catcatctgg cggtgcggta
      361 attgataaat cattgccccc accaatgtga ctgataaaat gccaggcaac acgacatcat
      421 ttatcaggat acgttatgaa accgaccacc attgcttcac ttcagaaata taagcaggat
      481 aaaaagcgtt tcgcgaccat caccgcttac gactacagct tcgcgaaact atttgctgat
      541 gaagggctta acgtcatgct ggtaggcgac tcgctgggca tgaccgttca gggccacgat
      601 tccactctgc cagtaaccgt tgctgatatc gcctaccaca ctgccgccgt acgtcgcggc
      661 gcaccaaact gcctgctatt ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
      721 gcctttgaaa acgccgcaac ggttatgcgt gccggtgcca atatggtcaa aattgagggc
      781 ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt
      841 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt acaggggcgc
      901 ggcaatgaag cgagcgatcg actactcagc gatgcattag ccttagaagc cgcgggggca
      961 cagctgctgg tgctggaatg cgtgccggtt gagctggcaa aacgtattac cgaagcactg
     1021 gcgatcccgg ttattggtat tggtgcaggc aacgtcactg acgggcaaat cctcgtgatg
     1081 cacgacgcct ttggtattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
     1141 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     1201 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     1261 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg
     1321 tgcctactat gggtaatctg cacaatggcc atatgaagct ggtcgacgaa gccaaagccc
     1381 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     1441 atctggcacg ttatccacgg accttgcagg aggactgcga gaagctaaac aaacgtaaag
     1501 tggatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccaca
     1561 cttacgttga cgttcctggc ctttcgacca tgctggaagg tgccagccgt ccgggacatt
     1621 ttcgcggcgt ttcgactatt gtcagcaagc tgttcaacct ggtccagccg gacatcgcct
     1681 gcttcggtga aaaagatttt cagcaactgg cgttgatccg caaaatggtt gccgatatgg
     1741 gcttcgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctaa
     1801 gttcccgtaa cggttatctg acggcggaac aacgcaaaat tgcgcctggt ctgtacaaag
     1861 ttttaagttc gattgctgac aaattgcagg ctggggaacg ggatctcgat gaaattatta
     1921 ccattgcggg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
     1981 atgccgacac attgctggaa gtttctgaaa ccagcaaacg ggcagtaatt ctggtagccg
     2041 cctggcttgg cgatgctcgc ctgatcgaca acaaaatggt cgagctggcg taatacttaa
     2101 ctggcgctac ggctgatggc gccagttatt aatttacccc acgtcatctg tcataacaca
     2161 ggtaacttaa ataaggttaa ttaccttatc aatctcagca agaggtaaat ttgccatctc
     2221 tgcaacgtct tcccgagaca ttcctttact tagaagacgc agggcgaatt cttgcctttc
     2281 ctcctgtctt ccctgctgaa tccccttctc aatccctttc tcttcaaacc actgcgccag
     2341 cgtcatcata gacttccctc ccgtttccct gtctctcagc acaccataaa acaaatccgc
     2401 ttgttcagta tgaccgcgtt gcagcatata gttttgcatg gcaactaact gacttccgct
     2461 agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc aataacatta agtcgcgctg
     2521 gcgaatatgt ttttgcagta gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc
     2581 atccggtgtg atggtgatat ccaccagcgg gaaaggactg ttatagacgc gtcgcgccag
     2641 ctccggcgag taaaacatat caaaccagca cattgagagc ggataaggtg tggcctcgcc
     2701 ctgataaaac agtatcggca ccaccagcgg cagcttatcg tggccagcct ccagatgccg
     2761 gtgcatggca gcaatagaat aacgcatcat gcgaaaggcc attttcttat ccggcttgct
     2821 ttggtgttca atcacaacat gcagataacc gggattgccc tgcatttgca tggaatagag
     2881 cacgtccgtg ctgtgacctt tcaggcactc ttcaatgaaa ctccccgact ctaaatgaag
     2941 cgtgttgaga tcacaaagtt cgcgtaattc cactggcaaa tgtatctcca gaaagtcgcg
     3001 agccgtctcc gcatgcatta aaaattgttt aaataccgcg tcatgcggtg tggtactcgg
     3061 tgcatccatc ttccctgtct tccgtgtggc cttgcgtgaa ggcagtatcc agtcaaggcg
     3121 tgctatcgtc agccacctct ttattgttta ccgagcagcg ttcataaaaa tatcaattcg
     3181 ttacaggcgt tacatggcac gcttcggcgc ggtacgcaaa ctgctgacgc aaatgccatc
     3241 agatgagtag acactcatca aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg
     3301 caccgcctgc ttcgttaacg acagggtaga aaggtagaag ttatgattcg cacgatgctg
     3361 cagggcaaac tccaccgcgt gaaagtgact catgcggacc tgcactatga aggttcttgc
     3421 gccattgacc aggattttct tgacgcagcc ggtattctcg aaaacgaagc catcgatatc
     3481 tggaatgtca ccaacggcaa gcgtttctcc acttatgcca tcgcggcaga acgcggttcg
     3541 agaattattt ctgttaacgg tgcggcggcc cactgcgcca gcgtcggcga tattgtcatc
     3601 atcgccagct tcgttaccat gccagatgaa gaagctcgca cctggcgacc caatgtcgcc
     3661 tattttgaag gcgacaatga aatgaaacgt accgcgaaag cgattccggt acaggttgct
     3721 tgattgttat ccctgcggct ggttactcac cagccgcgac atcttctcca gcgaatccgt
     3781 tcttaagata taaagtcgtt ttagtaacaa cggattatcc cccggtttta ctttgccttt
     3841 catggttgtc accgccaggt gaaatccggc atcgtttgct gccttcacgg ctttgtcatt
     3901 aaagccgcca aacggatacg aaagatacca gacatgtgga ttaaattgcg ccagagcgcg
     3961 gcgtgaacgt gcaaaatcaa acagaatatt gtgctcacta cggctcagta atatgggtcg
     4021 gcgataacca tctacccgat gcaaaaaatg ggtatgtgac tggaaatcaa aaacatcgcg
     4081 aatttcgtta agctcagaaa cgctcataaa ttgcagcgat tttgggttcc acttctgcgg
     4141 gtgacgtttg atgcgtgagg taacaataaa caccgtcgcc ttcatgccat attgtttcaa
     4201 cacaggatac gcatagcggc tcaccgactt gaggccatca tcaaaggtaa tcaccaccgc
     4261 tcgcgcaggg agattgatcg tattcttcac gtaaccttcc agctgagcca tgctcagtgt
     4321 cgcgtatccc ctgtcgcgca gccaggccat ttggttattg aaagcgcgta ccgaggtggt
     4381 cgtcgaagta tggcgaaaac gggtgttttc ttcgtcgcgc agaatatggt gataagttag
     4441 caccggcagg ccattatcgg gttgggcatc cagcgcgctg atataggcca gtcgatcgcc
     4501 aatacggatc tgataccagg tttgatttaa cctgtctttc agtttatgca aaatcgggta
     4561 gcgcaaattg tccgccagta ccccaaatgg cgcactgccc acactcggcg cgttgtagac
     4621 cggcgtatct ttccaggtaa ttaagttctg attactcagc ggcttgttga ggtcacccaa
     4681 accgtcttca actttttgtc gtccctgaac tggctcgaga tgccctttat cgataaaccc
     4741 tttaccaaag ccaaaattaa atgcgtaata acttgcggca gtgggctcca cggcaatgat
     4801 ttgtccggcc cgaatattcc ccacggtcac catcttgtca ctaatttgcg cccagaccgc
     4861 agcattttcg atggtttgca tataacgggc aggtaacgcg gcactgacac ttgcggtaaa
     4921 cagcatcagc aggagaataa cagcttgttt atacatgaaa aaataaccga gggtgaaaca
     4981 acgcttttat tgtagcaaaa gcgccatcag aatcgcgggt taatactcat acaaattatg
     5041 tttgaggacg aaaggcggat ttttttgttg ttggtgccaa agactactca cctccggccc
     5101 acccagttcg acaatacact cacgaaactc tgaactggtc atggtttcac ggcaagccat
     5161 catctgttca attaacggta acgtgacacc agaaatcact tcgcaacggg agtgtttgtg
     5221 gcttaataat gaagccacgc gatacggtgg cgctccggct atatcggtta agaagatgac
     5281 accctcacca gagtccgctt catgcagagc atcgcacatc atgcggctga gcatattaga
     5341 gcttaatccg cgccagaaat tcacggcccg gcactgaaga agtgccccat gttttttctc
     5401 cagcgcatcc agtatctctt gcgcccggtc atcgtgacag gtaattaccc aacctaacat
     5461 cgccttcctc cttagccgaa aatcagccta ccctgaccat tgttgataaa gggtgatagc
     5521 cgtcaaaact cctctccccc gaatccagag gagaggaaat agccttagct acgcaaacca
     5581 cgtccacgtt ggatcagcga ccaacagatc aaataaaacg ccacaataaa gaccaccagt
     5641 acgccaaagg tagtgaccag cggcacatca tttataccga ggaagccgta gcggaaacca
     5701 ctgatcatat aaacgattgg gttcaggtgc gacagccctt gccagaacgg cggcaacaaa
     5761 gtcagtgagt aaaagacccc gcccaaatac gtgagtggcg ttaacacaaa ggttggcacc
     5821 aggctgatgt catcgaacgt tttggcaaac acgccgttca gcaaacccgc aagggagaat
     5881 agcaccgccg tgagcaccag ggttaaggca acgaataccc acgaatgcac ctgaaacggc
     5941 acaaaaaaca gtgaaattgc cgtcacaaga atgccaacaa acagaccacg cgccacgcca
     6001 ccgccgacat atccggcaat aatgacgtga gtcggaaccg gcgctaccag cagctcttca
     6061 atattacgct ggaacttggc accaaaaaat gatgacgcga cgttagcgta ggcgttggtg
     6121 atcaccgaca tcatgatcag ccccggtacg atgaactgca tatagctgaa gccatgcata
     6181 tcgccaatac gcgaacctat caggttaccg aagataataa agtaaagggt catggtgatg
     6241 actggcggca ccagcgtctg cacccagata cgcataaagc gatggatctc tttcgcccag
     6301 atgcttttta gcgccaccca gtaaagatgc atcatgcgcg atctccttgt ttttcattaa
     6361 ccagtgaaac aaacagctct tccagacggt tagctttgtt acgcatactt aatacctgaa
     6421 tgccctgctc acttaactgc gtaaatacgc tgttgatccc ctgctcacgt agcacttcaa
     6481 cttccagcgt cgcggtatcg accagtcgat actgatagcc atcgagcttc ggtaacggac
     6541 ttttcggtgc gagatcgaga ataaaggttt ccgatttcag cttcgccagc agcgccttca
     6601 tcgaggtatt ttccaccagc tcaccatgtt gaataatgcc gatattgcgg cacagcattt
     6661 ctgcttcttc caggtagtgt gtggtaagaa tgatggtggt gcctttgtcg tttaaatcct
     6721 tcaaaaagcc ccacattgag cggcgaagtt caatatccac gcctgcggtc ggttcgtcga
     6781 gaatcagtag tttaggttca tgcattaacg cacgggcaat cattaaacgg cgcttcatcc
     6841 cgccagataa catacgcgca cgttcgttgc gttttcccca taaatcgagc tgcttaagat
     6901 atttttcgct gcggatgtac gcctctttgc gctccacacc gtagtaccct gcctgattca
     6961 ccacaatttg ctgcacggtt tcaaacgggt tgaagttaaa ttcctgcggc accagtccca
     7021 actgacgttt agcgttcacg acatctttct cgagatcgta accaaatacg ctgacccgcc
     7081 cggaggtttt atttaccaga gagctgataa taccgatagt ggtcgatttc ccggccccgt
     7141 tcggcccgag aagcgcataa aaatcacccg cttcgacctg caaatctatt ccacgaagcg
     7201 cctgaacgcc gcctggatag ttttttttaa gctgttgaag ttccagtgca atggtcataa
     7261 atttttactt accttacgtt cttacacttt atctgtggtt taaatcgtcc cggagttgcc
     7321 ctatattagc caaacgtaat tatttggtta caggtcgtta acctccatga aagacataga
     7381 tacactcatc agcaacaatg cactatggtc aaaaatgctg gtggaagagg atcccgggtt
     7441 ttttgagaaa ctggcacaag cgcaaaaacc gcgctttcta tggattggat gttccgacag
     7501 tcgcgttcct gcagaacgtt taaccggtct tgagccgggc gaactctttg ttcaccgtaa
     7561 tgttgctaac ctagtcattc acaccgacct gaactgcctt tccgtggttc agtatgcagt
     7621 ggatgtactc gaagttgaac acattattat ctgtggccac tacggttgcg gcggcgtaca
     7681 agccgccgtt gaaaacccgg aactggggct tatcaacaac tggctgctgc atatccgcga
     7741 tatctggttc aaacatagct cattgctcgg cgaaatgccg caagagcgcc gtctggatac
     7801 cttgtgtgaa ctgaacgtca tggaacaggt gtataacctg ggccactcca ccattatgca
     7861 atcagcgtgg aaacgcgggc agaaagttac cattcacggc tgggcctacg gcattcacga
     7921 cggcttgctg cgtgatctgg atgttaccgc caccaaccgc gaaacccttg agcaacgtta
     7981 ccgtcacggg atttccaacc tcaagctgaa acacgccaac cacaaataaa aatgccatgc
     8041 cggatgcaac acatccggca acttcacact tactcgtcca gcagaatcac tttgccaata
     8101 tacggcagat gacggtaacg ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc
     8161 gggatcgaga aaccgataaa ttcaaccggg acgttcactt cacgacggga cggtttatcc
     8221 agcagcgtac aaatcgccaa cgacttcggt tcgcgcaggc ttaagatctc acgcactttc
     8281 gacagtgtat tccccgagtc gatgatatct tcaacaatca gcacgtcctt gccacggata
     8341 tcttcatcca gatctttgag gattttcaca tcacgggtgg tggacatgcc gctaccgtag
     8401 ctggaggcgg tcataaagtc gacttcatga gatacctgaa cttcacggca caggtccgcc
     8461 ataaacataa atgagccacg cagcagaccc accagcacca tatcgctgcc gctgtctttg
     8521 taacgctcag taatctgacg acccagttcg gcgatacgcg ctttaatctc cgcttcgggg
     8581 atcattactt ctacagtatg tttcatatct ctaaccatat gatttaaaaa caaatcactt
     8641 cgcacgggca ggttttggca ccgcaacaaa gcgcaagacg cgcagtatac cagtaaaact
     8701 attcattcgc ggaatctgtt aataaagctc gaattgtgat gacggtcaca catgttaaaa
     8761 cctataatta attgctacta aaatattaat gaattgaaat ggtgtctctt tatggcaatt
     8821 aacaatacag cctcgcgacg attactcgtc acgctaacag ccctttttgc agcgctttgc
     8881 gggctgtatc tactcattgg cggaggctgg ctggtcgcga ttggcggctc ctggtactac
     8941 cctatcgctg gccttgtgat gctcggcgtc gcctggatgc tgtggcgcag taaacgcgcc
     9001 gcgctttggc tatacgcagc cctgctgctc ggcaccatga tttggggcgt ctgggaagtt
     9061 ggtttcgact tctgggcgct gactccgcgc agcgatattc tggtcttctt cggcatctgg
     9121 ctgatcctgc cgtttgtctg gcgtcgcctg gtcattcctg ccagcggcgc agttgccgca
     9181 ctggtggtcg cactgctgat tagcggtggt atcctgacct gggccggatt taacgatccg
     9241 caggagatca acggcacctt aagcgccgat gccacacctg ccgaagctat ctcccccgtt
     9301 gccgatcagg actggcctgc gtatggccgc aatcaggaag gtcaacgctt ttcaccactg
     9361 aagcaaattc acgccgataa cgtccacaag ctgaaagaag cctgggtgtt ccgtactggc
     9421 gatgtgaagc agccgaacga tccgggtgaa atcaccaatg aagtgacgcc gattaaagtg
     9481 ggcgataccc tttacctgtg taccgctcac cagcgcctgt ttgcgcttga tgccgccagc
     9541 ggcaaagaga aatggcatta cgatcctgag ctgaaaacca acgagtcttt ccagcacgta
     9601 acctgccgtg gcgtctctta tcatgaagcc aaagcagaaa cggcttcgcc ggaagtgatg
     9661 gcggattgcc cgcgtcgtat cattcttcca gtgaatgatg gtcgcctgat tgcgattaac
     9721 gctgaaaacg gcaaactgtg cgaaaccttc gccaataaag gcgtgctcaa tctgcaaagc
     9781 aatatgccag acaccaaacc gggtctgtat gagccgactt ctccgccaat tatcaccgat
     9841 aaaaccatcg tgatggccgg ttcagtcacc gataacttct caacccgcga aacgtctggc
     9901 gtgatccgtg gttttgatgt caacaccggg gagctgctgt gggcttttga tccgggcgcg
     9961 aaagatccga acgcaatccc gtcagacgaa cacaccttta cctttaactc accaaactcg
    10021 tgggcaccag cggcctatga cgcgaagctg gatctggtct atctgccgat gggcgtgacc
    10081 acgccggata tctggggcgg taaccgcaca ccggaacagg aacgttatgc cagctcgatt
    10141 ctggcgctga atgccactac cgggaaactg gcgtggagct accagaccgt tcaccacgac
    10201 ctgtgggaca tggatcttcc ggcacagccg acgctggcgg acatcaccgt taatggtcag
    10261 aaagtgccga ttatttacgc tccggcgaaa accggtaaca tttttgtgct cgatcgtcgt
    10321 aatggcgaac tggtggttcc ggccccggaa aaaccggttc cccaaggtgc tgccaaaggc
    10381 gattacgtta cccctactca accattctcg gaactgagct tccgtccgac gaaagatttg
    10441 agcggtgcgg atatgtgggg agccaccatg tttgaccaac tggtgtgccg cgtgatgttc
    10501 caccagatgc gctatgaagg cattttcacg ccgccatctg aacagggtac gctggtcttc
    10561 ccgggtaacc tggggatgtt cgaatggggc gggatttccg ttgatccaaa tcgtgaagtg
    10621 gcaattgcca acccaatggc actgccgttt gtttcgaaac tgctcccgcg tggtcctggc
    10681 aacccgatgg agcagccgaa agatgccaaa ggcacgggta cggaatccgg cattcagcca
    10741 cagtacggtg taccgtatgg tgtcacgctc aacccgttcc tctcaccatt tggtctgcca
    10801 tgtaaacagc cagcatgggg ttatatctcg gcgctggatc tgaaaactaa tgaagtggtg
    10861 tggaagaaac gtattggtac gccgcaggac agtatgccgt tcccgatgcc ggttccggtg
    10921 ccgttcaata tgggtatgcc gatgctgggc gggccaatct ccacggcggg taacgtgctg
    10981 tttatcgccg ctacggcaga taactacctg cgcgcttaca acatgagcaa cggtgaaaaa
    11041 ctgtggcagg gtcgtttacc ggcgggtggt caggcaacac cgatgaccta tgaagtgaat
    11101 ggcaagcagt acgtggtgat ctccgcaggc ggtcacggtt catttggtac gaagatgggc
    11161 gactatattg tggcttatgc cctgccggat gatgtgaaat aaccataatg cccggagaga
    11221 tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc atccccgtat
    11281 cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt gcgttgtgat
    11341 taaacttcac cagcacttca ctgacattgc cttctacctt aacggtatct ttccagcccg
    11401 cgcgatgagc cgctggcggt ttgccatttt ctgacaagat acggaactgc gtgccgtgaa
    11461 tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt tcgtactgcc
    11521 ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc ctgaccgttg attttattgg
    11581 catggtggaa atcgaacttc ccgccgtggt tcatatgatt catattgccg tgccccatat
    11641 ggcccatcat ctggctgtga tccattccgg ccatcgcctg atcgccatat ttctccatta
    11701 gcatctgcat ccccatcata tcgagcatcg ggtccataga aagttgcagc ttgcgtaccg
    11761 tcagcccttc cagcgaaggt aacgcaggca ggctacttaa tgtgtcaggc aaagcaccgg
    11821 aggcactaat agcaatcggc tgaatccgca ttaccggatg cggcttgtca aacggtgcaa
    11881 tcgccatccc catctggctg accggcagcg tcaccaggtc aaagggtttg ttgtcgttaa
    11941 cctccaccag cacttcaaaa cgctcgccca tcagcaccgg cagctcgctc accttcaccg
    12001 gttcaggtag cagaccaccg tcgctggcaa tcacatacag cgggcgattg tcgctggtgg
    12061 cgaaattgag cgagcgggca ttacagccat tgagcaaacg caggcgcagc caaccacgcg
    12121 gggcagcgtg ttgtgggtag attgcaccgt tggtcagcag cgtatcgcca aaccagccca
    12181 cggcggcggt catcacatcc agttgataat caatctgccc gtcggcgcta aatttcttat
    12241 cctgaacgat caccggaaca tcatcgatac cccactgttt tggcagcatt aatttcagga
    12301 tctcgtcatc ttcaatcacc accagaccag ccagccccat cgccacctgt cgcccggtct
    12361 tgccatgttg atgtggatgg aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca
    12421 ccgagcgctt gccacctggc ggaataattc cctgtgggcc gccgtcgacg tcacccggta
    12481 cttccagccc gtgccagtgc aacgtcgtct cttccgccag ttggttgtag atatcaaccg
    12541 ttaccgcttt gccgcgctgt aatttcaccg ccggccccag cagattgccg ttatagcccc
    12601 aggtagttgc agttttctcg ccaaaggtgg actgacctgc gccaatagtt aactgaatgc
    12661 gattacgggc atcggtcgtg agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa
    12721 agactgcacg gctccacagt ggcaaggctg aagccacacc cagtgcgacg gaatatttca
    12781 agaaatcacg acgttgcata gttatttcct tattcttaag caggcgaaac aaaatcaaac
    12841 gttgagcata gtccttcccc ttacgggaag gtcaagccgc agacacaatt taatcgacaa
    12901 taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc ttctttaagc ccctggtaga
    12961 agcaatgaag acgtttttca gaacagtgtt attcggcagc ctgatcgccg tttgcgcaaa
    13021 cagttacgcg ctcagcgagt ctgaagccga agatatggcc gatttaacgg cagtttttgt
    13081 ctttctgaag aacgattgtg gttaccagaa cttatctaac gggcaaattc gtcgcgcact
    13141 ggtctttttc gcccagcaaa accagtggga tctcagtaat tacgacacct tcgacatgaa
    13201 agctctcggt gaagacagct accgcgatct cagcggcatt ggcattcccg tcgctaaaaa
    13261 atgcaaagcc ctggcccgcg attccttaag cctgcttgcc tacgtcaaat aatccctgat
    13321 acctttttgt agaaatattg accgtgcatc cgcggtcaat gttagctatt atgttgcgcc
    13381 ctttttttac gggtgttaac aaaggaggta tcaacccatg gccgaaaaaa aacagtggca
    13441 tgaaacgcta cacgaccagt ttgggcagta ctttgcggta gataacgttc tgtatcatga
    13501 aaagaccgat caccaggatc tgatcatttt tgagaacgct gcatttggtc gcgtaatggc
    13561 gctggatggc gtagtacaaa ccaccgagcg cgacgagttt atctatcatg agatgatgac
    13621 ccatgttccg ctactggccc acggtcacgc gaaacatgtg ctgattatcg gcggcggcga
    13681 cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt gagtcaatca cgatggtgga
    13741 aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatctg cccaaccata acgccggtag
    13801 ctacgacgat ccgcgctttc aactagtgat cgacgatggc gtcaatttcg ttaatcaaac
    13861 cagccagacc tttgatgtca ttatctccga ctgcaccgat cctatcggtc ccggcgaaag
    13921 cctgttcacc tcagcatttt atgaaggctg caaacgttgc ctgaatcctg gcggtatctt
    13981 cgtcgcacaa aacggcgtct gctttttaca gcaggaagaa gccatcgaca gccatcgcaa
    14041 actcagccat tacttcagcg acgttggctt ttatcaggcg gcgatcccga cctattacgg
    14101 cggtatcatg acttttgcat gggcgacaga taacgacgcc ttacgccatc tctcaaccga
    14161 aattattcag gcgcgttttc tcgcttctgg cctgaaatgc cgttattaca atccggcaat
    14221 ccatacggca gcttttgcct tacctcagta tctgcaagac gcactggctt cacagccgtc
    14281 ctaaggagaa gataagaaat tgaaaaaact gaaactgcat ggctttaata atctgaccaa
    14341 aagtctgagt ttttgtattt acgatatctg ctacgccaaa actgccgaag agcgcgacgg
    14401 ttatattgct tatatcgatg aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga
    14461 aacctgttcc attatcgggg ccaatattct taacatcgcc cgccaggatt acgaaccaca
    14521 gggtgccagc gtcactattc tggtgagtga agaaccggtt gacccgaaac tcatcgacaa
    14581 aacagaacac cccggcccac tgccagaaac ggtggttgct catctcgata aaagtcatat
    14641 ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga
    14701 tattgaagtc tctacctgcg gcgtgatttc tccgctgaag gcgctgaatt acctgatcca
    14761 ccagcttgag tccgatatcg taaccattga ttatcgcgtg cgcggtttta ctcgcgacat
    14821 taacggtatg aagcacttta tcgaccatga gattaattcg attcagaact ttatgtctga
    14881 cgatatgaag gcgcggtatg acatggtgga tgtaaacgtc tatcaggaaa atatcttcca
    14941 taccaagatg ttgcttaaag agttcgacct taagcactac atgttccaca ccaaaccgga
    15001 agatttaacc gacagcgagc gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga
    15061 gatttattac gggcgcaata tgccagctgt ttaacggctc tggcggagct cccaggctcc
    15121 gccagattta tttacttctg ctgcacgaaa ttgcggtaag ccgccacgac ctgcagaaaa
    15181 tcctcaacgc cgcatagcga caggctttct tcgtcgtagt agttcatccc ctcttccatt
    15241 tcatcgccag cgaattccag ttgattggcg cgaaccatca cctcttcagc gtccatccac
    15301 agggtgtatt catgccctgc ccgttgccag gaccgttcgc tacctttcag tgcgtgcgct
    15361 gcttgttcca cttcatcaag caaggccagg ttttctttca cctcttcatt aaaccagtgc
    15421 ccgaccactt catgccccat ggacatacgc acctttacca ctccggtaat atcgcgcaga
    15481 aattcgtaat ccatagtgtg ttcctctgct cccggcaatg cgccgaaatc gcatcgcgcc
    15541 attgatgtaa ttatcgcagc cttaccggag aaaaacagcg gaacggcgat agtttagaaa
    15601 taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg
    15661 cttacttatt acaccgcagt ctggaaaatc accccatcgg ctttttcggt gtactgagaa
    15721 agctggttga agttcagata acgataagta tcaacggcag ttttatctac ttgcgcaacg
    15781 taggtctggt actcttccgg cgtcggcagt ttgccaatca gcgccgcaac agccgccagt
    15841 tctgcagaag ccaggaagac attcgcgcca gtacccaaac ggttcgggaa gttacgggta
    15901 gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct ggttacccat acacagggaa
    15961 cagcccggga tctcgatacg agccccgctc ttaccgaaga cgctgtagta gccttcttcc
    16021 gtcagttgcg cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactga
    16081 cctttgtgcg catccagcag tttaccagcg gcacggaagt gaccgatgtt agtcatgcaa
    16141 gaaccgataa atacttcgtc gatcttctcg ccctgtaccg cagacagcgg acgtgcgtca
    16201 tccggatcgt tcggagcaca caggattggc tctttaatct ccgccagatc gatgtcgatc
    16261 actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt
    16321 tccatgccct gaatacggcg ttccagggta cgacgatcgc cgtaaccttc cgcgatcatc
    16381 cacttcagca ggacgatgtt ggagctcagg tattcgatga tcggttcttt gttcagcttg
    16441 atagtacaac cagcggcaga acgctcggcg gacgcatcgg ttagctcaaa ggcctgctca
    16501 actttcagat ccggcagacc ttcaatttcc agaatgcgac cagagaagat gtttttcttg
    16561 cctttcttct caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc
    16621 agatcgcgca gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc
    16681 ggcatatcca gcggcattac gccagttgcg gcagcaaacg ccaccagacc agaacccgcc
    16741 gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc
    16801 ggcagcagca tacggttcag ccaggagtga atgacgccgt cacccggacg cagcgacaca
    16861 ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc
    16921 ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac
    16981 gccaggtctt tcagttcatc acgggtcatc gggccggtgg tgtcctgaga gcctacagaa
    17041 gtcattttcg gttcgcagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct
    17101 accattttct gcgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg
    17161 aacacatcac tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgcccg
    17221 ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt
    17281 tcgaaggtcg ccagcagttc gcctgtttcg tggttacgca cttcaccttt gtacgggtaa
    17341 acgtcaatca cgtcgcccat gttcaggtta gagacgttga cttcgattgg cagtgcaccc
    17401 gcgtcttcca tcgtgttaaa gaagatgggt gcaattttac cgccgaggca caaaccaccg
    17461 ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac ggagttcgtg
    17521 gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct
    17581 ttctgttgca gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca
    17641 ataccttcac gggcgttttt cagcatcgcc agcgcgtgca gtgggatatc cgggcgtgac
    17701 cacgcatccg gtgccggaga aaggtcatcg gtgttggttt cgccagtgac tttaaagacg
    17761 gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc atccgcccag
    17821 gactgcatta cctgcttcgc atattcgttg cctgctttcg ctttctcttc tacgtcatag
    17881 aagttatcga acatcagcag cgtgtgagaa agtgctttgg cagcaatagg tgccagtttg
    17941 gcatcatcca gcgcgtcgat cagcggatga atgttgtaac caccctgcat ggtgcccagc
    18001 agttcgatag ctttttccgg agtcagcaga ggggatttag cttcgccttt agcgatagca
    18061 gccaggaagc ctgcttttac ataggcggct tcatcgacgc ctgggggaac acggttggtt
    18121 aacagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagc
    18181 gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct
    18241 acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg
    18301 caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg
    18361 caacgggcag aatagcagga ttttgacgac gtgttaatct gtttacaaaa aagcaacata
    18421 aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt
    18481 taattcgcat ggtgataatc acctttctca acgaatccca agattagtca aaatttaaac
    18541 taccgcctct ttatactcgg attcacagca cctgcgggtg gcagttcgcc gaccattgcg
    18601 atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc
    18661 taatttgcag tgccgggctt tgtgctgcct ctgccgggct atatataaaa agccgcacag
    18721 tggaagcgcc tgtagaaccg caatcgacac aactggctgt gtctgacgct gccgctgtta
    18781 cgcttcctgc aacggtttcc gcacctcccg taatacccgc agtcgtcaaa tccgcattca
    18841 gcactgcaca aatagatcaa tgggtcgcac ccgtcgcgct gtatcccgac gccctacttt
    18901 cgcaggtgct gatggcatca acctatccga caaacgttgc tcaagcagtg caatggtcgc
    18961 acgataatcc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg
    19021 acgccagcgt taaatcactg gtggcctttc cacaattgat ggcattgatg ggcgaaaacc
    19081 cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca gccgcaggac gtgatggact
    19141 cggtacagcg attgcggcaa ctggcgcaac aaaccggctc gctgaagtca tcaaccgaac
    19201 agaaagttat taccacaacg aagaaagctg taccggtaaa acagacagtc acggcacccg
    19261 tcataccatc caataccgtt ttaactgcca accccgtcat tacagagccg gcaacaaccg
    19321 tcatttccat tgagcccgcc aatcctgatg tggtctatat tcccaactac aacccaaccg
    19381 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
    19441 gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta tagcatgggc gttgctacca
    19501 cgtacgcact attcagcagc atcgactgag atgacgacga tcatgaccat catcatcatg
    19561 acgatgatga ttatcatcac cacgatggcg gtcatcgtga cagtaatggc tggcaacaca
    19621 acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta
    19681 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    19741 atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg agtgccacgc
    19801 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    19861 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    19921 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    19981 cactgaccca gcagcaaaag gacgccgctc gtcagcgtta tcagtcggcc tctcctgagc
    20041 agcgccaggc agtccgcgag aaaatgcaga ctaacccgca gatccagcag cgaagggagg
    20101 cggcgcgcca gcgtattcag tccgcctcgc ctgagcagcg ccaggcggtt cgcgagaaaa
    20161 tgcagactaa cccgcagaac cagcagcgaa gagaggcagc gcgtgagcgt attcagtctg
    20221 caactcccga acagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    20281 agcaacgtga taacgcccgc cagcgtatac aatcggcatc acctgaacaa cgtcaggttt
    20341 ttcgggagaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
    20401 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgaacgt ggagcaagaa
    20461 gactggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    20521 ccggatgttc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt
    20581 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    20641 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    20701 acccagcagc tcgccgccgt tggtaccaac aatcgcacca ccgatcacac ggtgagattc
    20761 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtca gaagcgatag cacgaccaga
    20821 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    20881 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    20941 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    21001 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    21061 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt
    21121 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    21181 acgctgcggt tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    21241 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    21301 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    21361 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    21421 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    21481 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    21541 aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa cttccagggt
    21601 gttagccccg gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    21661 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    21721 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    21781 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    21841 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    21901 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    21961 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    22021 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    22081 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    22141 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    22201 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    22261 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    22321 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    22381 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    22441 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    22501 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    22561 ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    22621 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    22681 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    22741 cgacagcgaa ctattgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    22801 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    22861 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat
    22921 catcacccag ttacggctca ggttagcacc agaaattttc tggatacggc ccagttccac
    22981 ttcttcgatt tcgccaaact tgctgaagtc caccttcggc cacggcagca tgccagggat
    23041 accaccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    23101 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    23161 aacgccaaac tcgcgtgcca gacggcggat cagcggggtc gcgtgaacgt aagcgtcgtt
    23221 ttcagcaaat tcagatttgc cttccgcttt cgcagccgga gcttcagctt ttgctgccgg
    23281 agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc
    23341 gaagatcata atcagcgaac cagttttcac tttatcgcca acgttgactt tcagttcctt
    23401 cacgacgcct gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtaat
    23461 cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac
    23521 ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagctgctg gtgcaggggt
    23581 cgctgccgga gtcgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac
    23641 ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc
    23701 tttcacggta cctgcaaacg gagccggaac ttccatagaa gccttgtcgc cttctacggt
    23761 gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc
    23821 aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc
    23881 tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc
    23941 gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac
    24001 tttgatctct ttaacgatac ccgcctgcgg agatggaact tccatagagg ctttgtcgcc
    24061 ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc
    24121 ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct
    24181 tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt
    24241 gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca
    24301 accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg gctgtcggaa
    24361 cgaccgaagc catcagtacc cagtacgcga tagtcgtcag ccggtacgta agtacggacc
    24421 tgctcagcga acagtttcat atagtcggta gatgccactg ccggcgcgtc gttcatcacc
    24481 tgagcgatat acggaacgcg cggagtttcc agcggatgca gcatgttcca gcgttcacaa
    24541 tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc agaacctacg
    24601 ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat ggaaccggag
    24661 cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta
    24721 cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta
    24781 gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc
    24841 ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc
    24901 agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta
    24961 cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtctccagc cgcccagcac
    25021 agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaatgg gatcatcggc
    25081 agattgttcg tgctgtaaga ggtcgccgct gccagccagg aacaccctgc gcccagctcg
    25141 ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg
    25201 cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga
    25261 ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc
    25321 gacttgttct tcagcatcac gttcagagca cgaacgaaag cgatagtggt agagagctct
    25381 ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg
    25441 gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga
    25501 taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca
    25561 tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc
    25621 ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg
    25681 gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg
    25741 tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag
    25801 tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca
    25861 ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc
    25921 agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg
    25981 atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta
    26041 ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaaaac caggttatcc
    26101 agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg
    26161 tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg gtgttccaga
    26221 tatttcagga atttagcctg gtaaatagca ccaatcggac ccagacccat agatacggtc
    26281 gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg
    26341 tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca
    26401 cgggcgtaaa cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc
    26461 tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg
    26521 aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg
    26581 atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac
    26641 tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt gcctgcggct
    26701 acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc
    26761 tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc
    26821 ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt
    26881 tgagttttct ggaacctgtc ccattgaact ctcgccagaa agctcaataa gacaggttct
    26941 acgtttagtt accgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta
    27001 ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct
    27061 ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc
    27121 ttcgcgcgct tcttccggct taccgaccat aatcgcttca aatatgctgg tgcggtgact
    27181 actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt
    27241 ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc
    27301 ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca gatcgccaga
    27361 ctgctgcgcc agctctatgg cgtggtggag ttcacggatg cgttccttgt cttcatcggt
    27421 actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa
    27481 gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg
    27541 ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc
    27601 ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag
    27661 ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc
    27721 cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag
    27781 ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc
    27841 taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt
    27901 gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtag
    27961 actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc gaattgaacc
    28021 gattcactta ccaatttttt gatttttaag tcaataaaaa cgaatttaaa ttcactctgc
    28081 atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca actcatcttc
    28141 aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat ctgacggaag
    28201 tctttttgta acaattcaaa cctccttgat gtaaacaaat caatacaaca aacggaattg
    28261 caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg
    28321 atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag
    28381 cttatcgcgc tgggtggcgc gatagggaca gggctattcc taggtagcgc ctccgtaata
    28441 cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg
    28501 atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac
    28561 tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta
    28621 ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat tcagttctgg
    28681 tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat taacgccatc
    28741 aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt
    28801 attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg caacggcggc
    28861 ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca cggcttcacc
    28921 ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga actggtgggg
    28981 atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcaac taaccaggtt
    29041 atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc actgatgccg
    29101 tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga gttaggcgat
    29161 acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc cgtgtacaac
    29221 agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg gaacgcgcca
    29281 aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct ggtgtctgcg
    29341 ctggttacag cattgtgcgt attgattaac tatcttgctc cggaatccgc atttggcctg
    29401 ttaatggcac tggtggtatc cgcactggtg atcaactggg cgatgatcag tctggcgcat
    29461 atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt
    29521 tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg
    29581 atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt
    29641 atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac
    29701 gccctcaccc gtacagggtg agggcaataa tctctacacc aacgccccgt aaatcgtcag
    29761 tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa ccgctgcctt
    29821 cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg ccagacgcgt
    29881 taatacctga tattgcgaag tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc
    29941 tttctcacca tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc
    30001 cagtacatga agcactgcat caatgccgga ctgtaaacga tgatgcggcg tctggtatcg
    30061 cgccagccag tattgccatg cacgcaagaa cgcatacccc atcagcgtaa cgggtcccca
    30121 ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt taatccacag
    30181 caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg
    30241 aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc
    30301 tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat
    30361 cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag
    30421 taaaaaagtc acacccatcg caataatggt catgcctaac gtgcgcccga gagaaaaatg
    30481 tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct cgcccaactt
    30541 aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg
    30601 ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt tttccgatcc
    30661 ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat cgggatagcg atctatcagt
    30721 gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag agtatcggtg
    30781 ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cacgcccctg atactgagag
    30841 actcccgcat gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcacgg
    30901 cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc
    30961 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
    31021 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccag gcggcaatcg
    31081 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
    31141 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
    31201 cgttagctat ctggagtttt aacatgccgc ctcgccgcta caaccctgac acccgacgtg
    31261 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
    31321 aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa
    31381 attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtattttgt ggcaaacgct
    31441 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
    31501 atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg
    31561 tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca
    31621 gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg ttggatacgc
    31681 gcaaaacctt acctggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag
    31741 cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgcct
    31801 ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcatccggat gcgccagtag
    31861 aagtcgaagt agagaatctg gaagaacttg atgaggccct gaaagccgga gccgatatca
    31921 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
    31981 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa
    32041 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt
    32101 caatgcgttt tcgctaattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc
    32161 ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgaaata
    32221 aaaaacctcg gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt
    32281 ttgccaaagt agcaccaacc aaatcaagga gcgaaacaga tggacaagca acgcggtttt
    32341 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagctc cattggtatt
    32401 cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg
    32461 ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
    32521 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
    32581 gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg
    32641 acgccaggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg caatattcaa
    32701 agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa
    32761 ggagcggcaa tgaatattcc acagctcacg gccctgtgcc tgcgttatca gggagtcttg
    32821 ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc acatgagttg
    32881 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
    32941 caaatggaag gtcacgccag tcgcacacaa cagacattac ccgtagctgt tcaggagaag
    33001 catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga acaacgcgcg
    33061 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc
    33121 gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac agccagatta
    33181 aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
    33241 gtcgaactgg caggaaacac cgtctcattt cgtattgcga ccttaccatg tcggggtggt
    33301 gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt taacacgctg
    33361 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
    33421 gtgctggtaa ctggccctac aggcagtggc aaaacggtca cctggtgttg tccacgctac
    33481 acaccaattc caccactgaa acgctggtac gtttacagca aatgggggtc gcccgctgga
    33541 tgctatcatc ggcgcttacg ctggtaatag cccagcgtct ggtacgtaaa ctttgcccac
    33601 attgtcgccg acagcaaggg gagcccatcc atattccaga cactgtatgg ccgtcgccgc
    33661 tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgtaccg
    33721 ccttgtttga agtcctgccc attacaccag ccatacgcca gcttatttcc gctaatactg
    33781 aagttgaatc gctggaaacg cacgcccgac aggcgggtat gcgtacgctt tttgaaaacg
    33841 gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtactgggga
    33901 tgccgcatgg caagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg
    33961 caagatggga tgctatgggc agagagccgt actttactgc ttatggcact acagcaacag
    34021 atggttaccc cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat
    34081 aaaagcgcgg aagtcattca tcaactggcg acgctactta aagccgggtt aacgctttct
    34141 gaagggctgg ctctgctggc ggaacagcat cccagtaagc aatggcaagc gttgctgcaa
    34201 tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt accctggtca
    34261 gaggcatttc cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg
    34321 gatgaatgct gctttgaact ggcgcgtcag caaaaatccc agcgtcagtt gaccgacaaa
    34381 gtgaaatcag cgttgcgtta tcccatcatc attttagcga tggcaatcat ggtggttgtg
    34441 gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt caacacccca
    34501 ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga atggggctgg
    34561 ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat gcgccgcccg
    34621 acctggctta tcgcgcggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg
    34681 aggggacaaa aactcacgca gatctttacg attctggcgc tgacacaaag tgcaggcatt
    34741 acttttttac agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa
    34801 cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct ggcgctaaaa
    34861 aatgccggtg agtttagccc gctctgtttg caattagtga gaacaggaga ggcatccggc
    34921 tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg
    34981 gcggataacc tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc
    35041 ggtacgctgg tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtggg
    35101 atgggataac gctggcgtgt tgctccacgc cagcgttggg agattacagg ttgttgaaga
    35161 tgcggttttc ttgttccagc acacgaataa acgtggtgcg cttggtcagc tctttcaggc
    35221 gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat
    35281 tttcaacctg gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg cggtattccg
    35341 caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag aacagcataa
    35401 atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc
    35461 cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt cgccacatcg cccggcgtgg
    35521 tacagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg gcacattcga
    35581 ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac
    35641 ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc tcacacattt
    35701 caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg
    35761 caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa acgaagttta
    35821 atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg
    35881 tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact
    35941 cttcgacaga aaagtgttta tgcacagcag tcagaatatc aaaagaagcc agcgcagagg
    36001 ccatagaaaa tgtgcctacg gtgtccatat ttgcggcgat aatcggcacg ccggaccagc
    36061 tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa
    36121 gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa
    36181 tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac
    36241 gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac
    36301 aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc actttcgcgc
    36361 gctcattttt aacgttttga taatcatgat gaattcccgg gaatataaat tatggggtat
    36421 atagttgcct taacgggagg cattggcagt ggtaagagta ccgttgccaa tgcgtttgct
    36481 gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt
    36541 gcacctgcgc tacatgccat tgctgatcac tttggcgaaa acatgattgc tgctgatgga
    36601 acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg
    36661 cttaacgacc tgctgcatcc gctgattcag caagagacgc aacaccagat ccagcaagca
    36721 acttccccct atgtactgtg ggttgtgcca ttgctggtag aaaactcact gtataaaaaa
    36781 gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag
    36841 cgcgatgatg taactcgcga acatgtcgaa caaatccttg ctgctcaggc aacgcgcgaa
    36901 gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca
    36961 tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag
    37021 gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta
    37081 catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc atcgttgacc
    37141 acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt ttcgagcgcg
    37201 gcgaagtccg cactgagctg ttgaaagaac ttaaccggca gcaacgtaaa ctccagacct
    37261 ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag cagttaaaag
    37321 cggcggtgag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt gaagatcgtt
    37381 tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc tttgatttac
    37441 ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag gtagaaacct
    37501 ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat ttaattcgcc
    37561 agtcggcccc cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg
    37621 atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg caaatttccg
    37681 gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac ggacaggtac
    37741 cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta
    37801 cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc
    37861 cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct gaagaaaaac
    37921 gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt
    37981 gacatttgtc tgatgcctca cgtaggcctg ataagacgcg gacagcgtcg catcaggcat
    38041 cttgtgcaaa tgtcggatgc ggcgtgaacg ccttatccga cctacagacg cttaagcttc
    38101 gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac cagcgacatc
    38161 cactcaccgg gttgcccttc tttaccccac ggcacccctt cccaactttc gaccagccag
    38221 aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa
    38281 tgttgtgggg taatcccgac ctcttcctga agttcacgca ccaccgcctg ttccggcgtt
    38341 tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg cgcatctgct
    38401 gcgcgacgtg ttataaagat ttcattgttc tcgttgcgaa taatacctac cgcaatttgc
    38461 agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa
    38521 ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttacca gaaccgcacg
    38581 ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt gcagctgcgg
    38641 ctgcagagtc gtcatcctga tggctaagct gctgcatttg tgctaaacgc tcggcttcca
    38701 tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga actttgctca
    38761 gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg gagaacgatt
    38821 cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg ataccctgac
    38881 gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt tgcagcatga
    38941 cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta cgctgataca
    39001 cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt tctggttctt
    39061 tatccagcca ctcggtaatt ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca
    39121 gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca tcaatggtgg
    39181 ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg acatccagca
    39241 gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca tcatattcca
    39301 gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg ttggcaatcg
    39361 ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt ttacgcatca
    39421 tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc gacaggtaga
    39481 aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg ttatcgatac
    39541 gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc agtaccgcat
    39601 cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc ggattttcca
    39661 gcgcggcaac ttctgcctgc cagctacctc cgagcacaat atctgtacca cgacccgcca
    39721 tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc gccgcttcgt
    39781 tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc agttcgtttg
    39841 acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg cctttcgcag
    39901 tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc atgtagacca
    39961 ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg gtatccagtt
    40021 tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc cccgccagtt
    40081 tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg ttttcgttct
    40141 ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac cagcgtcggc
    40201 cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt
    40261 agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca
    40321 gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc tctttcacca
    40381 gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga gatttttcgt
    40441 ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc tgacggatca
    40501 ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct gccgggccgg
    40561 aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg tccaccagcg
    40621 catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg tcgcgcaggt
    40681 agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa gcttcgcgct
    40741 ttgccggtgc tggcatgccc ggcaggttga taccaacagt caggccaagg aattcaaaca
    40801 gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta actacgtgca
    40861 cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg gttttacctt
    40921 caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg agtaactgaa
    40981 cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgcaccacg gcgaaagctt
    41041 ccgggatcag attttccagc acttcgcctt tttccagacg cgcacgaaac tctgcggttt
    41101 tccctttcag ttcttcatcg gagagttttt ccatctccgg ttccatggca ttgatgatgt
    41161 tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa actttagtta
    41221 acaatttgat tagcataata aaatctcaaa cgccccgcgt tacggagtta ataaaatgaa
    41281 gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc ttgcgcctgg
    41341 cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata atcaatgcga
    41401 taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt atccagtaat
    41461 gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagtgt ttgcggtgcc
    41521 attgcgaaag aaagatgacg gattaccgta cgaatagcat gttgatgcca gtaatcaacg
    41581 gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg accaaagtta
    41641 actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg
    41701 gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa gagatgcggc
    41761 cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac gttattgcca
    41821 tcccgatcga attcaaacgt atttagcgca caaatcttat cattaaagcc tgaagtccac
    41881 agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt
    41941 tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg agagagtgcc
    42001 agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt acagctgaag
    42061 gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat tcccaggctt
    42121 cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg gatttataag
    42181 cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc gcatcgagca
    42241 ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat
    42301 cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg gactgcagat
    42361 attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc ataaacgcat
    42421 cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga tggttaaaat
    42481 cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac ttatcgccat
    42541 cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc aactcgtcga
    42601 taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg atcgggattt
    42661 ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga gcagcattga
    42721 ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta cagagcatgg
    42781 tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag tcggtgcgac
    42841 gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg
    42901 tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg
    42961 tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc gggccagttt
    43021 agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg cttacgtagg
    43081 aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt gtcattcact
    43141 actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg ctggtagcga
    43201 tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg acgtttgtcc
    43261 atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc cgggtcaaga
    43321 gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt gttacctacc
    43381 gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag cacgccgcgc
    43441 gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc agcagcttct
    43501 tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc catttcagac
    43561 attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt aatcagttcg
    43621 gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc atccagcagg
    43681 gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc
    43741 acatgcttgg acagttcagt gatcccctgc tcggcgaatg ccatacgctt cttgccttca
    43801 aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt tgccacttca
    43861 gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc aataaagacc
    43921 atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc cgcattgcgg
    43981 ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc gatttgaatc
    44041 gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc gaagaattca
    44101 acaccttcaa tacgctcgcg caccatgtgt tcaacagcat taccgccgcc gccgccgacg
    44161 ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat agtttctctc
    44221 cgatttgtgc ctgtcgcctg agtccgtaat catcgtcggc ctcataaaaa ttaaaactct
    44281 tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt
    44341 tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa tcccaccgcc
    44401 gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag cggcgcgccg
    44461 atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc gatctgcgct
    44521 gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc
    44581 ttttcctgca actgcaatat ctcttcgttg accaggttga gcaactcggt atagcgcggc
    44641 tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc gcctacgctc
    44701 ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc gtggcgaact
    44761 ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc actggtcacg
    44821 acattgccag cataaggaat tacctttgtg tggcgcaatg ccccaccggt ataaacggcg
    44881 atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg ttcatcttcc
    44941 gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac tttcagccca
    45001 caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt gatcaggtgc
    45061 acttttgcct gcatccgcac acccgaaagt cctaccggat tcttgatccc ttcctgatag
    45121 tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg cacacgcacc
    45181 gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc ttcagaaata
    45241 ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc cagatatacc
    45301 gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg tacgcacttg
    45361 accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg gcagctgccc
    45421 acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc ggcaaccttc
    45481 gcggtaccaa tttccagtcc tactaccagt tttctgtccg tcgccttgat cattgttgtt
    45541 ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc gcccagccta
    45601 ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg
    45661 cctgctgctg taaaaccgga taaagttcta caaagcgaac caaacgtttc atcgtatcgc
    45721 cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa cgccgcgcgg
    45781 tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc atttcgcgat
    45841 agccctgcaa cacttcatta gcgctgcctt ccgggccata cagcattgga agcacctgct
    45901 tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc atgtgttgat
    45961 cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca tcaggccact
    46021 gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc gtctggatga
    46081 tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc gactgccgga
    46141 tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag agcggcaggc
    46201 gttgcgcatc ttccatccag ctcaacacga cccagccgct caccaacact gtcgttaaaa
    46261 cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga gaagaaacct
    46321 cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc cagttccaga
    46381 attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat cggcaccagg
    46441 ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg tccatcactg
    46501 tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca tgctttcagc
    46561 actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca gaaatactgt
    46621 gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg ttgaatacgt
    46681 attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc acttagccat
    46741 ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc ttgtagagca
    46801 ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg cttaacgata
    46861 accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc tttttcaaac
    46921 tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg ccacagaagt
    46981 ttgctgcgta gtttatctat tgaaagcgca gatgccatca ctccgctgcc ggtataaggc
    47041 aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg accgtgtagc
    47101 gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac ttctttcggg
    47161 tcgacaggat acgcatcaat accgccttca cgcagcccgg ctaacaccgc tgcgccagaa
    47221 ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat tttatcagtc
    47281 atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag aacgggcaat
    47341 ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta ataccggtgc
    47401 cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt tcccacgtcc
    47461 acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt cgccagccgg
    47521 atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga aatcatcata
    47581 caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt ttttatccgg
    47641 ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt ggccgtagtc
    47701 atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga attcaccgag
    47761 gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa tagcctcgtc
    47821 gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg
    47881 gcctggcgca ttcagggtga cgcacatcgg ctctttgtcc tgacgcagca gcgtaaagtg
    47941 cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt cgctgaagcc
    48001 gtaagttgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg gatcatcaac
    48061 acacatcacc gcacgaccgt aaaaaggcag gttgtgcaga aaattaataa aggtctgttt
    48121 taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat tggtgacaat
    48181 cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt cggcaatcag
    48241 gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc cgccgttaac
    48301 gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg tagttgtcgt
    48361 tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact cagccagcat
    48421 ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt ccgggttgtc
    48481 ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt ccgggcgatg
    48541 gttgaaataa atcgtcgcac ccagattcag taactgctgc gtgaccggat ttggcgctaa
    48601 atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac cgcccatacc
    48661 ggcaccacca atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga
    48721 acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct taaattcata
    48781 cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc atttgccact
    48841 cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt
    48901 tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg tggctgctcg
    48961 ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg ctggcggtct
    49021 ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc actcacagtt
    49081 aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc catatcatca
    49141 ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg ttcaacggat
    49201 tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag tttcgcagca
    49261 acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac taccaacaca
    49321 cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc cagcacatcg
    49381 gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa cgcctgcatc
    49441 actttggtag caatcttcgc cagccattta ttggttaagc ccgcaatacc gttttgttca
    49501 tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc tgatacatag
    49561 ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg cgcctgacgc
    49621 caggcgttga agatacgcag cggcgcagct atcagtgctt ttataccttt tccacgcaaa
    49681 ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc cgcttccata
    49741 cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagata gtgcgcaacc
    49801 gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa tcgctttcct
    49861 tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc gtttcataat
    49921 caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa ccaccgtaac
    49981 tgattagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg cctacgttaa
    50041 ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg gaaaaacggt
    50101 ggtcaatttc taatgctttg cggccaatcg acatcgcacg aaaagcgacg aagaatacca
    50161 ttaaaagtgc tagtaccaca ccgacataac ctaattcttc gccgataatg gcgaaaataa
    50221 agtcagtgtg cgcttccggc agatactcca gtttttgtac cgaattacct aaaccttgcc
    50281 cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag ccgctgccaa
    50341 agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac ggttcggcga
    50401 ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac tgccacaatt
    50461 tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc accgtaccaa
    50521 ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc ggtttcagga
    50581 agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg gcgatatagc
    50641 aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg agatcgatcc
    50701 aacgcgatgc ccctttaaca gcgctaccca ctaccagcac gatcatcagc aggatgatag
    50761 atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa
    50821 tgatcgccag aataaacgcc aaaatcagat agacaccatc acgcttcgcg aagaagaacg
    50881 gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg ataaagccaa
    50941 tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc aggctgtcgg
    51001 tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtggagatc cagaccagga
    51061 tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc atcaacctaa
    51121 ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga actgatcaag
    51181 gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg gagccagcaa
    51241 gcgcatagcc tgttccatag tttcggtttg ttctgccact tccgggcgta gcgccgccag
    51301 ctgcgcgccg tcacgaccga aacaatacag acgtacgtta tcgccattca ggtaacgcac
    51361 cagtgggcta aagtccgccg atttaccatc gccgcccagc aacaaatgca gcgtgccgtc
    51421 tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt
    51481 aatccagcgc acaccgttat gctccagcac cacttcaaag cgatgcggca gaccggtgaa
    51541 ggtggttaac gctttcaggc tgctggcgcg cggtaaccct gcggcatctg ccagcgccag
    51601 cgccaccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt tcacattcag
    51661 cactttctcg cctttaaccc gcagccaggt ttcgccctgc tgatgattca ggtgatagtc
    51721 acccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa
    51781 ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca gttttgctgc
    51841 acgatactgt tgtaaaccaa acgggtagcg atccatatga tcttcagtca cgttcagaat
    51901 ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg acagttccag
    51961 cacgtacagt tcacactcat catccagtag catcaacgca ggcaggccaa tattgccacc
    52021 cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg tgaccgtgct
    52081 tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac aaaacagctc
    52141 gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg gatgcgccag
    52201 tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat cattcagact
    52261 gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg tcatacgcgt
    52321 atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg aaagcccggt
    52381 gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga ttaacgtacc
    52441 ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa acgcacaatg
    52501 acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc catgcggaaa
    52561 atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag cgtttctacc
    52621 acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac ggcgataatg
    52681 cctaacgcac cacctaacgc cagcgaaccg acatcgccca taaagacctg cgccggatag
    52741 gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac aataaccagt
    52801 tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat gttgccggtc
    52861 gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat tgccagaccg
    52921 tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa gtaagccagc
    52981 agaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac caccagctgc
    53041 gttgcgggcg tgtctttgcc ggcaaggtac aaagcgaaag caacacccag cgcaatgacc
    53101 gacatccaga aatatttcca acgagcgatc aaccctttgg tgtctttacg caccactttg
    53161 cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa cacgcaccag
    53221 acgtacggat tggacgggta agcccacagc agcacggaga tcacaatcgc tgtcaggatc
    53281 ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta
    53341 cgcactacct gaccaaagga aagtttttgc aaatgagcaa tcatgcgcgg gcccatccac
    53401 aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag ataggaaaag
    53461 acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa catgtcccat
    53521 tctcctgtaa agcgcgtact acctcttcca tggcagcact acgtgaaccc ttaactaaaa
    53581 tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac gcagttttat
    53641 cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt ttacccatgc
    53701 ttaacacgcg gtcaatacca gcagcttttg ccgcctcacc cacctgtaca tggcaggctt
    53761 cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag cccggcattt
    53821 cagccagtac ctgaacagct gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga
    53881 gcagcaactg gttttctgcc agttggatgg ggaacagacg gccaggaaca gctttcagat
    53941 ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg
    54001 ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca tcgacgctac
    54061 cggttggggt ttgtagggta aattccatac cgtgcgaggt cacatggata ttggtggcgg
    54121 tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag ccaattacgc
    54181 tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg ttttccggca
    54241 ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca aaaccttcca
    54301 gatgcgccgc tgccaggttg ttgaccagcg ccgcttccgg gcgagtcaga ctcacagtcc
    54361 aggcaatctc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg
    54421 ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct gccgtataaa
    54481 gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag gttttgccgg
    54541 aggaccccgt cagagcaacc acgagcgccg gaacttgctg gcgaacccat gcagccagtt
    54601 caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgatg tccagcggac
    54661 ggctaaccag tagtgcgcct gcgccgccag ctttcgcctg gtcggcaaaa tcgtgggcat
    54721 caaaacgttc acctttcagg gcaacaaaca ggcagcccgg cgtcagtttt cgggtatcag
    54781 tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga atatcggtaa
    54841 gttggctaag ggttacgcta atcatgcaat cccccccagc agacgcgcca ccgtgacgcg
    54901 atcggagtag tccagacgct gattgccaac aatctggtaa tcctcatggc ctttgcccgc
    54961 gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg cttcagcacg
    55021 gccttccatc actttggcat gtccggcatc taacattccc gccagaatat cgttgatgat
    55081 ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt cagcaaactc
    55141 ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac cacagccaaa
    55201 gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt tttccagtgc
    55261 atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag tgaacacttc
    55321 catacgtccg caaaccggtt gcagacgcgc ggcggttttc agcagatcag ccagtggata
    55381 gcccagcgcc agcagtgttg ccagcgcgag caacaggttg ctgacgttaa aagcgcccat
    55441 cagatggctt tcaatttcgc catcgcccca gcttgaacta aagcgaatgg tcgcgccgct
    55501 gtcgtgatag ttcacttcgg tcgctttcaa ccagcgtccg tgacagttcg gattgatatg
    55561 atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc ggcccacttc
    55621 atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca gccatttcgc
    55681 agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta agttggtaaa
    55741 gaccgacgcc ggaaatttca atgccgccac acggtgctgt accagcccgt gggaggaaac
    55801 ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat gctgaacatc
    55861 aactgccgaa ccggtagtat tttctgtcgg gatcactttc cccagcagac cgttaccaac
    55921 ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca gaagctgggt
    55981 cgtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt cagagggttc
    56041 atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga gatagatgac
    56101 cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat
    56161 aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg
    56221 acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc
    56281 tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc gcaaattacg
    56341 atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg cccgttgtca
    56401 gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca ccaaagaccg
    56461 gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata acaacaacca
    56521 gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata tatttattga
    56581 tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg atacgatagc
    56641 ctttaatcgc cgccttcacg ccgccgccgc ctggtagcgc cacgctttcc atcatatgca
    56701 ccacggtgcg aacaatggat tccgggaaga cacgttcacc gggaaccggg gggtcaactt
    56761 tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag actcgcgcta
    56821 actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc tctatgtcag
    56881 accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc aaattggtcg
    56941 cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc atcgttaacg
    57001 ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg gttaattcgc
    57061 tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg gtattgagta
    57121 ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc ggtttaaccg
    57181 ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct ttcggcgtgc
    57241 cgctcagatt gttagggttg tatgacgggc tgttagccat cgccagcact tcacctgtgt
    57301 tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc gcgttgttca
    57361 gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg ttgtgcgctg
    57421 cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct ttacgcacaa
    57481 tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg ccctcaatcc
    57541 cttgactatc gacgttagta aagccgatga ggtgagcagt cacttcgccg gacggatagt
    57601 aacggcgaga ctcttcacgc agatgaatcc ccggcagttt cagttttttg atgtagtccg
    57661 ccatgtcaga gtttacctga cgcgccagat aaataaagcg ccctttcggg ttggcgttaa
    57721 tgcgggcaga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc ttccagcggt
    57781 caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa atcgctttta
    57841 ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg cgggaggtgg
    57901 aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata tccggggaga
    57961 taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga atacagccgc
    58021 ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt tttggtttct
    58081 gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact acgatatttt
    58141 cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt tccacccggc
    58201 tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg tctaaagcat
    58261 ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg
    58321 ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc agcttcccaa
    58381 atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg
    58441 atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg tcctctctgc
    58501 aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt cgcccggcat
    58561 taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct cttcagtcat
    58621 cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac gtttcacaat
    58681 acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg ccagcacgtt
    58741 gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc aaatgcgcac
    58801 cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg ttgcagcagc
    58861 cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac gctcgacaat
    58921 ggcgcgggca atgcgtttgg caaaatgctc ttcaccatag gttttcaata cccaggcgat
    58981 atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg ttgggtccat
    59041 acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat catcaagttg
    59101 cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccaataa gatcgcgctc
    59161 ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc gcggatcatc
    59221 aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc
    59281 ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat
    59341 gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca gcaccgtagt
    59401 atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc gataagtctc
    59461 cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag gttgtttcat
    59521 cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc agcccggcat
    59581 gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga cattcgctgg
    59641 catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc aacagacgcg
    59701 ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg cacgggtgat
    59761 gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt tcccgataac
    59821 gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct ccccggaaca
    59881 tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa ggagtttacg
    59941 gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa aacccgatgt
    60001 ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa ttaacgttat
    60061 gaatagctgt aagaaaaaat ccctcatcgt catcactgtt ttaaaatgac tcactatttt
    60121 ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac atatcttccc
    60181 cggttaacag tccttgttac gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc
    60241 aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat aatgcaaaaa
    60301 cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat taacgggcgg
    60361 gtttaaaacc atttatcaat aatagtcagg aatagtctta tttactttaa ggatattgat
//
LOCUS       DAJTNP010000013        59925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTNP010000013 DAJTNP010000000
VERSION     DAJTNP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="OO160_000895"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="OO160_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5523118.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="OO160_000896"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="OO160_000896"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5523119.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="OO160_000897"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="OO160_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5523120.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO160_000898"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO160_000898"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5523121.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGDGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO160_000899"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO160_000899"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5523122.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="OO160_000900"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="OO160_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5523123.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="OO160_000901"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="OO160_000901"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5523124.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEASACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="OO160_000902"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="OO160_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="OO160_000903"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="OO160_000903"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5523125.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="OO160_000904"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="OO160_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5523126.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO160_000905"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO160_000905"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5523127.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="OO160_000906"
     CDS             complement(13538..13933)
                     /locus_tag="OO160_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5523128.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO160_000907"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO160_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5523129.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="OO160_000908"
     CDS             complement(15220..15447)
                     /locus_tag="OO160_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5523130.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="OO160_000909"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="OO160_000909"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5523131.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="OO160_000910"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="OO160_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5523132.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO160_000911"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO160_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5523133.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="OO160_000912"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="OO160_000912"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5523134.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="OO160_000913"
     tRNA            19688..19764
                     /locus_tag="OO160_000913"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            19798..19874
                     /locus_tag="OO160_000914"
     tRNA            19798..19874
                     /locus_tag="OO160_000914"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19832..19834,aa:Met,seq:cat)
     gene            complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="OO160_000915"
     CDS             complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="OO160_000915"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5523135.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21434..22765
                     /gene="argA"
                     /locus_tag="OO160_000916"
     CDS             21434..22765
                     /gene="argA"
                     /locus_tag="OO160_000916"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5523136.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22827..24653)
                     /gene="recD"
                     /locus_tag="OO160_000917"
     CDS             complement(22827..24653)
                     /gene="recD"
                     /locus_tag="OO160_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5523137.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24653..28195)
                     /gene="recB"
                     /locus_tag="OO160_000918"
     CDS             complement(24653..28195)
                     /gene="recB"
                     /locus_tag="OO160_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5523138.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="OO160_000919"
     CDS             complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="OO160_000919"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5523139.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31252..34620)
                     /gene="recC"
                     /locus_tag="OO160_000920"
     CDS             complement(31252..34620)
                     /gene="recC"
                     /locus_tag="OO160_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5523140.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34633..34983)
                     /locus_tag="OO160_000921"
     CDS             complement(34633..34983)
                     /locus_tag="OO160_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5523141.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34968..35375)
                     /locus_tag="OO160_000922"
     CDS             complement(34968..35375)
                     /locus_tag="OO160_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5523142.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35372..35935)
                     /locus_tag="OO160_000923"
     CDS             complement(35372..35935)
                     /locus_tag="OO160_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5523143.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35926..36396)
                     /locus_tag="OO160_000924"
                     /pseudo
     CDS             complement(35926..36396)
                     /locus_tag="OO160_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="OO160_000925"
     CDS             complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="OO160_000925"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5523144.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="OO160_000926"
     CDS             complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="OO160_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5523145.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="OO160_000927"
     CDS             complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="OO160_000927"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5523146.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="OO160_000928"
     CDS             complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="OO160_000928"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5523147.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41881..42570
                     /gene="mutH"
                     /locus_tag="OO160_000929"
     CDS             41881..42570
                     /gene="mutH"
                     /locus_tag="OO160_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5523148.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42639..43352
                     /locus_tag="OO160_000930"
     CDS             42639..43352
                     /locus_tag="OO160_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5523149.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43490..43708
                     /gene="ygdR"
                     /locus_tag="OO160_000931"
     CDS             43490..43708
                     /gene="ygdR"
                     /locus_tag="OO160_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5523150.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43816..44856
                     /locus_tag="OO160_000932"
     CDS             43816..44856
                     /locus_tag="OO160_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5523151.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="OO160_000933"
     CDS             complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="OO160_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5523152.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46072..48231)
                     /gene="aas"
                     /locus_tag="OO160_000934"
     CDS             complement(46072..48231)
                     /gene="aas"
                     /locus_tag="OO160_000934"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5523153.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48817..49848
                     /gene="galR"
                     /locus_tag="OO160_000935"
     CDS             48817..49848
                     /gene="galR"
                     /locus_tag="OO160_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5523154.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="OO160_000936"
     CDS             complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="OO160_000936"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5523155.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51239..52174
                     /gene="lysR"
                     /locus_tag="OO160_000937"
     CDS             51239..52174
                     /gene="lysR"
                     /locus_tag="OO160_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5523156.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="OO160_000938"
     CDS             complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="OO160_000938"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5523157.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52982..54400)
                     /gene="araE"
                     /locus_tag="OO160_000939"
     CDS             complement(52982..54400)
                     /gene="araE"
                     /locus_tag="OO160_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5523158.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="OO160_000940"
     CDS             complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="OO160_000940"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5523159.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="OO160_000941"
     CDS             complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="OO160_000941"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5523160.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56629..57810)
                     /locus_tag="OO160_000942"
     CDS             complement(56629..57810)
                     /locus_tag="OO160_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5523161.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58065..59294
                     /locus_tag="OO160_000943"
                     /pseudo
     CDS             58065..59294
                     /locus_tag="OO160_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14799 a        16075 c        14589 g        14462 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccatca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagcctc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggtgtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg acaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc
    19801 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19861 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat
    19921 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
    19981 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    20041 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    20101 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    20161 tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag
    20221 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    20281 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    20341 ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg
    20401 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    20461 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    20521 cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
    20581 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    20641 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct
    20701 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    20761 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    20821 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg
    20881 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    20941 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
    21001 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    21061 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    21121 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    21181 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    21241 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    21301 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    21361 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc
    21421 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    21481 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    21541 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    21601 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    21661 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    21721 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct
    21781 caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    21841 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    21901 tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt
    21961 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    22021 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    22081 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    22141 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    22201 agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct
    22261 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    22321 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    22381 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    22441 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    22501 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    22561 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    22621 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    22681 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    22741 agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac
    22801 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    22861 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    22921 gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    22981 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    23041 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    23101 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    23161 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct
    23221 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca
    23281 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    23341 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    23401 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    23461 aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    23521 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    23581 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    23641 ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg
    23701 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    23761 cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc
    23821 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    23881 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
    23941 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    24001 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    24061 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
    24121 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    24181 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    24241 gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc
    24301 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    24361 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    24421 agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc
    24481 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    24541 gcgagggtga cggcaggatg ttcatctccc gccacagtca gggcaaattg cacatccagc
    24601 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    24661 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    24721 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    24781 taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    24841 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    24901 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    24961 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    25021 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    25081 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    25141 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    25201 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    25261 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    25321 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    25381 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    25441 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    25501 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    25561 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    25621 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    25681 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    25741 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    25801 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
    25861 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    25921 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
    25981 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
    26041 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    26101 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    26161 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
    26221 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
    26281 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
    26341 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    26401 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    26461 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    26521 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    26581 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    26641 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    26701 tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    26761 tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct
    26821 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
    26881 catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    26941 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    27001 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    27061 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    27121 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    27181 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt
    27241 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct
    27301 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
    27361 tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac
    27421 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    27481 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    27541 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac
    27601 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    27661 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    27721 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
    27781 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
    27841 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    27901 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    27961 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    28021 gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    28081 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    28141 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    28201 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    28261 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    28321 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    28381 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    28441 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    28501 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    28561 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    28621 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    28681 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    28741 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    28801 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    28861 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    28921 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    28981 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    29041 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    29101 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    29161 gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat
    29221 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg
    29281 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    29341 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    29401 gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg
    29461 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat
    29521 ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa
    29581 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    29641 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    29701 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    29761 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    29821 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    29881 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    29941 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    30001 tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    30061 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg
    30121 ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag
    30181 ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga
    30241 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    30301 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    30361 ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct
    30421 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    30481 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    30541 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    30601 gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg
    30661 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    30721 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    30781 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    30841 tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca
    30901 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    30961 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat
    31021 ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    31081 ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc
    31141 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    31201 tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg
    31261 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    31321 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    31381 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    31441 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    31501 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    31561 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg
    31621 tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca
    31681 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    31741 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    31801 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    31861 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    31921 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    31981 gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    32041 ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    32101 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    32161 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    32221 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    32281 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    32341 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    32401 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    32461 taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    32521 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    32581 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    32641 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    32701 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    32761 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    32821 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    32881 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    32941 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    33001 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    33061 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    33121 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    33181 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    33241 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    33301 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa
    33361 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    33421 ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    33481 atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    33541 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    33601 agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg
    33661 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    33721 cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    33781 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag
    33841 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    33901 aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg
    33961 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    34021 cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg
    34081 tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    34141 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    34201 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    34261 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    34321 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    34381 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    34441 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    34501 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    34561 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    34621 agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    34681 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    34741 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    34801 ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat
    34861 acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag
    34921 caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga
    34981 cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt
    35041 tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat
    35101 aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc
    35161 gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac
    35221 tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg
    35281 cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc
    35341 agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc
    35401 tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca
    35461 ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat
    35521 ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt
    35581 ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat
    35641 cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa
    35701 tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg
    35761 cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta
    35821 aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga
    35881 tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc
    35941 tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc
    36001 gcccgttgag tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa
    36061 agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa
    36121 tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt
    36181 cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa
    36241 atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca
    36301 gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag
    36361 cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa
    36421 aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg
    36481 caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc
    36541 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    36601 aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    36661 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    36721 cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa
    36781 atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    36841 aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag
    36901 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    36961 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    37021 ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    37081 ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat
    37141 ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag
    37201 ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg
    37261 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    37321 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    37381 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    37441 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    37501 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    37561 caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    37621 aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    37681 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    37741 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    37801 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    37861 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    37921 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    37981 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    38041 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    38101 ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc
    38161 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    38221 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    38281 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    38341 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    38401 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    38461 aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    38521 tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    38581 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    38641 catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc
    38701 cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt
    38761 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    38821 cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    38881 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    38941 caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag
    39001 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    39061 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    39121 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    39181 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    39241 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc
    39301 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    39361 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    39421 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    39481 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    39541 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    39601 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    39661 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    39721 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt
    39781 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    39841 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    39901 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    39961 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    40021 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    40081 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    40141 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    40201 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    40261 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    40321 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    40381 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    40441 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    40501 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    40561 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    40621 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    40681 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    40741 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    40801 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    40861 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    40921 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    40981 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    41041 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    41101 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    41161 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    41221 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    41281 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    41341 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    41401 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    41461 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca
    41521 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg
    41581 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    41641 atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    41701 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    41761 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    41821 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    41881 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    41941 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg
    42001 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    42061 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    42121 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta
    42181 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    42241 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    42301 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    42361 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    42421 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    42481 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    42541 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    42601 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    42661 atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    42721 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    42781 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct
    42841 cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    42901 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    42961 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    43021 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg
    43081 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    43141 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    43201 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    43261 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    43321 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    43381 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    43441 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    43501 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    43561 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    43621 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    43681 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    43741 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    43801 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    43861 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    43921 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    43981 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    44041 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    44101 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    44161 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    44221 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    44281 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    44341 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    44401 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    44461 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    44521 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    44581 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    44641 gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt
    44701 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    44761 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    44821 gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc
    44881 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc
    44941 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    45001 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    45061 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    45121 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc
    45181 agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    45241 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    45301 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    45361 agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc
    45421 gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc
    45481 atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg
    45541 gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag
    45601 tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc
    45661 attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat
    45721 ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg
    45781 ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc
    45841 cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca
    45901 cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt
    45961 aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg
    46021 ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc
    46081 gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc
    46141 aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc
    46201 gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag
    46261 caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg
    46321 cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt
    46381 ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat
    46441 atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac
    46501 ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag
    46561 ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg
    46621 tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg
    46681 cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag
    46741 ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa
    46801 gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac
    46861 ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata
    46921 aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga
    46981 gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt
    47041 aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc
    47101 ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc
    47161 caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc
    47221 ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag
    47281 tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat
    47341 agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg
    47401 cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat
    47461 gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag
    47521 caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc
    47581 gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat
    47641 acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc
    47701 acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg
    47761 gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc
    47821 aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta
    47881 gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg
    47941 gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg
    48001 aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta
    48061 aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga
    48121 gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac
    48181 gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc
    48241 ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc
    48301 gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag
    48361 ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt
    48421 ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg
    48481 tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct
    48541 gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct
    48601 gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt
    48661 tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc
    48721 tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact
    48781 gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc
    48841 cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc
    48901 agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac
    48961 gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat
    49021 gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc
    49081 ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt
    49141 gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat
    49201 gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg
    49261 cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg
    49321 cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt
    49381 tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg
    49441 gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg
    49501 accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg
    49561 gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg
    49621 ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg
    49681 cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat
    49741 aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca
    49801 gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag
    49861 caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc
    49921 attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc
    49981 gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc
    50041 gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg
    50101 cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag
    50161 ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc
    50221 atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa
    50281 tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc
    50341 caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc
    50401 aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg
    50461 taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg
    50521 ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg
    50581 ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc
    50641 gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca
    50701 tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt
    50761 caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt
    50821 aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat
    50881 ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg
    50941 aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat
    51001 ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa
    51061 acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac
    51121 aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc
    51181 gatttatgtc gagtctatgc aaaaatgata tgaattaccg gattgcgaga gagcgctaat
    51241 ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct
    51301 gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg
    51361 ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac
    51421 cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat
    51481 tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct
    51541 gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc
    51601 cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc
    51661 ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac
    51721 cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa
    51781 aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga
    51841 cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat
    51901 cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc
    51961 ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt
    52021 cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc
    52081 gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct
    52141 tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc
    52201 gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct
    52261 gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca
    52321 taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga
    52381 ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc
    52441 agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga
    52501 gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga
    52561 gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata
    52621 ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta
    52681 tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc
    52741 atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag
    52801 taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc
    52861 tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc
    52921 aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa
    52981 atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca
    53041 gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca
    53101 gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg
    53161 ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat
    53221 cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca
    53281 tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg
    53341 aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca
    53401 tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg
    53461 caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt
    53521 ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca
    53581 taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt
    53641 tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt
    53701 cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg
    53761 cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca
    53821 gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc
    53881 tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact
    53941 ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag
    54001 gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca
    54061 tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg
    54121 ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa
    54181 ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa
    54241 agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca
    54301 atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag
    54361 aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag
    54421 taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata
    54481 aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta
    54541 agttgaatta ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag
    54601 ttcattaatt gataattgat atggattatt tcataaccat gatatggatt atgatgatct
    54661 agagatataa aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg
    54721 cgccagccaa ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga
    54781 ggcaagaaac actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat
    54841 gcgatcgaga atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt
    54901 cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc
    54961 catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat
    55021 cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc
    55081 gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat
    55141 gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc
    55201 gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg
    55261 aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt
    55321 gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc
    55381 ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc
    55441 aactttacct tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc
    55501 cacaactagc gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca
    55561 ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag
    55621 ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc
    55681 cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc
    55741 cggcgctcgt gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc
    55801 agccccatac tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg
    55861 cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact
    55921 tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg
    55981 gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg
    56041 cggtgaccga tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg
    56101 ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc
    56161 ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg
    56221 tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg
    56281 cgcagccctt gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc
    56341 acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt
    56401 cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac
    56461 aatgttttat ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat
    56521 gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc
    56581 aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg
    56641 ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg
    56701 ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc
    56761 tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc
    56821 aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc
    56881 gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc
    56941 aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac
    57001 gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc
    57061 gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa
    57121 acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa
    57181 cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc
    57241 aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct
    57301 gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc
    57361 atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc
    57421 ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat
    57481 gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc
    57541 ggaaccgcaa acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat
    57601 agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac
    57661 ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact
    57721 accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg
    57781 taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata
    57841 gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa
    57901 ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa
    57961 cccaccaata gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa
    58021 attgccctcc attttgttta atttgtagat gatacgttca gataatgtct aatatttggt
    58081 ctaaagaaga aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc
    58141 tattccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg
    58201 tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa
    58261 aaacatcagc aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg
    58321 gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag
    58381 tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca
    58441 tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac
    58501 gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat
    58561 ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt
    58621 tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta
    58681 gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg
    58741 ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac
    58801 tgttctttgt ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag
    58861 aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt
    58921 caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact
    58981 ttggcgttat tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa
    59041 agaaaagtcg tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca
    59101 ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca
    59161 ttgccatgat acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac
    59221 cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa
    59281 tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc
    59341 atttttcata atattcattt gttattattc cgccctatca cttctctcgt catatcctag
    59401 aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga
    59461 tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat
    59521 attctcatac catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg
    59581 aaacataatc atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag
    59641 caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat
    59701 catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc
    59761 tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc
    59821 cagaagagtc accgcgtttg cagtactgcg caaactcgtg ttttgatctt acccagcaat
    59881 agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAJTNP010000014        58529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTNP010000014 DAJTNP010000000
VERSION     DAJTNP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1053)
                     /gene="ycjM"
                     /locus_tag="OO160_000944"
     CDS             complement(<1..1053)
                     /gene="ycjM"
                     /locus_tag="OO160_000944"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5523162.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPE"
     gene            complement(1265..1612)
                     /gene="pspE"
                     /locus_tag="OO160_000945"
     CDS             complement(1265..1612)
                     /gene="pspE"
                     /locus_tag="OO160_000945"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5523163.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1655..1876)
                     /gene="pspD"
                     /locus_tag="OO160_000946"
     CDS             complement(1655..1876)
                     /gene="pspD"
                     /locus_tag="OO160_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5523164.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1885..2244)
                     /gene="pspC"
                     /locus_tag="OO160_000947"
     CDS             complement(1885..2244)
                     /gene="pspC"
                     /locus_tag="OO160_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5523165.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2244..2468)
                     /gene="pspB"
                     /locus_tag="OO160_000948"
     CDS             complement(2244..2468)
                     /gene="pspB"
                     /locus_tag="OO160_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5523166.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2522..3190)
                     /gene="pspA"
                     /locus_tag="OO160_000949"
     CDS             complement(2522..3190)
                     /gene="pspA"
                     /locus_tag="OO160_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5523167.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3357..4334
                     /gene="pspF"
                     /locus_tag="OO160_000950"
     CDS             3357..4334
                     /gene="pspF"
                     /locus_tag="OO160_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5523168.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4454..5719)
                     /gene="puuE"
                     /locus_tag="OO160_000951"
     CDS             complement(4454..5719)
                     /gene="puuE"
                     /locus_tag="OO160_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5523169.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5757..7037)
                     /gene="puuB"
                     /locus_tag="OO160_000952"
     CDS             complement(5757..7037)
                     /gene="puuB"
                     /locus_tag="OO160_000952"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5523170.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7039..8526)
                     /gene="puuC"
                     /locus_tag="OO160_000953"
     CDS             complement(7039..8526)
                     /gene="puuC"
                     /locus_tag="OO160_000953"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5523171.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8676..9233)
                     /gene="puuR"
                     /locus_tag="OO160_000954"
     CDS             complement(8676..9233)
                     /gene="puuR"
                     /locus_tag="OO160_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5523172.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9260..10024)
                     /gene="puuD"
                     /locus_tag="OO160_000955"
     CDS             complement(9260..10024)
                     /gene="puuD"
                     /locus_tag="OO160_000955"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5523173.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10236..11654
                     /gene="puuA"
                     /locus_tag="OO160_000956"
     CDS             10236..11654
                     /gene="puuA"
                     /locus_tag="OO160_000956"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5523174.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11803..11967
                     /locus_tag="OO160_000957"
     CDS             11803..11967
                     /locus_tag="OO160_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523175.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11957..13342
                     /gene="puuP"
                     /locus_tag="OO160_000958"
     CDS             11957..13342
                     /gene="puuP"
                     /locus_tag="OO160_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5523176.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13476..13721
                     /locus_tag="OO160_000959"
     CDS             13476..13721
                     /locus_tag="OO160_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5523177.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14034..15677
                     /gene="sapA"
                     /locus_tag="OO160_000960"
     CDS             14034..15677
                     /gene="sapA"
                     /locus_tag="OO160_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5523178.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15674..16639
                     /gene="sapB"
                     /locus_tag="OO160_000961"
     CDS             15674..16639
                     /gene="sapB"
                     /locus_tag="OO160_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5523179.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16626..17516
                     /gene="sapC"
                     /locus_tag="OO160_000962"
     CDS             16626..17516
                     /gene="sapC"
                     /locus_tag="OO160_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5523180.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17516..18508
                     /gene="sapD"
                     /locus_tag="OO160_000963"
     CDS             17516..18508
                     /gene="sapD"
                     /locus_tag="OO160_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5523181.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18510..19316
                     /gene="sapF"
                     /locus_tag="OO160_000964"
     CDS             18510..19316
                     /gene="sapF"
                     /locus_tag="OO160_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5523182.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19384..19737
                     /locus_tag="OO160_000965"
     CDS             19384..19737
                     /locus_tag="OO160_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5523183.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20105..20893
                     /gene="fabI"
                     /locus_tag="OO160_000966"
     CDS             20105..20893
                     /gene="fabI"
                     /locus_tag="OO160_000966"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5523184.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21037..22164
                     /locus_tag="OO160_000967"
     CDS             21037..22164
                     /locus_tag="OO160_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5523185.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22232..24166
                     /gene="rnb"
                     /locus_tag="OO160_000968"
     CDS             22232..24166
                     /gene="rnb"
                     /locus_tag="OO160_000968"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5523186.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24244..24348)
                     /locus_tag="OO160_000969"
     CDS             complement(24244..24348)
                     /locus_tag="OO160_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523187.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            24402..26376
                     /gene="pdeR"
                     /locus_tag="OO160_000970"
                     /pseudo
     CDS             24402..26376
                     /gene="pdeR"
                     /locus_tag="OO160_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26524..26697
                     /locus_tag="OO160_000971"
     CDS             26524..26697
                     /locus_tag="OO160_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523188.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26787..27536
                     /locus_tag="OO160_000972"
     CDS             26787..27536
                     /locus_tag="OO160_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5523189.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27805..28023
                     /gene="osmB"
                     /locus_tag="OO160_000973"
     CDS             27805..28023
                     /gene="osmB"
                     /locus_tag="OO160_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5523190.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28149..28475)
                     /gene="yciH"
                     /locus_tag="OO160_000974"
     CDS             complement(28149..28475)
                     /gene="yciH"
                     /locus_tag="OO160_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5523191.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28475..29212)
                     /gene="pyrF"
                     /locus_tag="OO160_000975"
     CDS             complement(28475..29212)
                     /gene="pyrF"
                     /locus_tag="OO160_000975"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5523192.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29405..30574)
                     /gene="lapB"
                     /locus_tag="OO160_000976"
     CDS             complement(29405..30574)
                     /gene="lapB"
                     /locus_tag="OO160_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5523193.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30581..30889)
                     /gene="lapA"
                     /locus_tag="OO160_000977"
     CDS             complement(30581..30889)
                     /gene="lapA"
                     /locus_tag="OO160_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5523194.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31038..31802)
                     /gene="pgpB"
                     /locus_tag="OO160_000978"
     CDS             complement(31038..31802)
                     /gene="pgpB"
                     /locus_tag="OO160_000978"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5523195.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31972..32562
                     /gene="ribA"
                     /locus_tag="OO160_000979"
     CDS             31972..32562
                     /gene="ribA"
                     /locus_tag="OO160_000979"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5523196.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32626..35301)
                     /gene="acnA"
                     /locus_tag="OO160_000980"
     CDS             complement(32626..35301)
                     /gene="acnA"
                     /locus_tag="OO160_000980"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5523197.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35465..35560)
                     /locus_tag="OO160_000981"
     CDS             complement(35465..35560)
                     /locus_tag="OO160_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523198.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35674..35841)
                     /locus_tag="OO160_000982"
     CDS             complement(35674..35841)
                     /locus_tag="OO160_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523199.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35844..36008)
                     /locus_tag="OO160_000983"
     CDS             complement(35844..36008)
                     /locus_tag="OO160_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5523200.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36303..37277)
                     /gene="cysB"
                     /locus_tag="OO160_000984"
     CDS             complement(36303..37277)
                     /gene="cysB"
                     /locus_tag="OO160_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5523201.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37487..40084)
                     /gene="topA"
                     /locus_tag="OO160_000985"
     CDS             complement(37487..40084)
                     /gene="topA"
                     /locus_tag="OO160_000985"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5523202.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40464..40715
                     /locus_tag="OO160_000986"
     CDS             40464..40715
                     /locus_tag="OO160_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5523203.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40751..41800)
                     /gene="sohB"
                     /locus_tag="OO160_000987"
     CDS             complement(40751..41800)
                     /gene="sohB"
                     /locus_tag="OO160_000987"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5523204.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42020..42778
                     /locus_tag="OO160_000988"
     CDS             42020..42778
                     /locus_tag="OO160_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5523205.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42775..43365
                     /gene="cobO"
                     /locus_tag="OO160_000989"
     CDS             42775..43365
                     /gene="cobO"
                     /locus_tag="OO160_000989"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5523206.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43405..44277)
                     /gene="rluB"
                     /locus_tag="OO160_000990"
     CDS             complement(43405..44277)
                     /gene="rluB"
                     /locus_tag="OO160_000990"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5523207.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44378..44998)
                     /locus_tag="OO160_000991"
     CDS             complement(44378..44998)
                     /locus_tag="OO160_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5523208.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44995..45876)
                     /gene="yciV"
                     /locus_tag="OO160_000992"
     CDS             complement(44995..45876)
                     /gene="yciV"
                     /locus_tag="OO160_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5523209.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46014..46058
                     /gene="trpL"
                     /locus_tag="OO160_000993"
     CDS             46014..46058
                     /gene="trpL"
                     /locus_tag="OO160_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5523210.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46035..46128
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46150..47712
                     /gene="trpE"
                     /locus_tag="OO160_000994"
     CDS             46150..47712
                     /gene="trpE"
                     /locus_tag="OO160_000994"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5523211.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47712..49307
                     /gene="trpD"
                     /locus_tag="OO160_000995"
     CDS             47712..49307
                     /gene="trpD"
                     /locus_tag="OO160_000995"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5523212.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49308..50669
                     /gene="trpCF"
                     /locus_tag="OO160_000996"
     CDS             49308..50669
                     /gene="trpCF"
                     /locus_tag="OO160_000996"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5523213.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50681..51874
                     /gene="trpB"
                     /locus_tag="OO160_000997"
     CDS             50681..51874
                     /gene="trpB"
                     /locus_tag="OO160_000997"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5523214.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51874..52680
                     /gene="trpA"
                     /locus_tag="OO160_000998"
     CDS             51874..52680
                     /gene="trpA"
                     /locus_tag="OO160_000998"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5523215.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53060..53239
                     /locus_tag="OO160_000999"
     CDS             53060..53239
                     /locus_tag="OO160_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5523216.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53325..53825
                     /locus_tag="OO160_001000"
     CDS             53325..53825
                     /locus_tag="OO160_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5523217.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53871..54377
                     /locus_tag="OO160_001001"
     CDS             53871..54377
                     /locus_tag="OO160_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5523218.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54437..55075)
                     /gene="ompW"
                     /locus_tag="OO160_001002"
     CDS             complement(54437..55075)
                     /gene="ompW"
                     /locus_tag="OO160_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5523219.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55315..55404
                     /locus_tag="OO160_001003"
                     /pseudo
     CDS             55315..55404
                     /locus_tag="OO160_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55432..56175
                     /locus_tag="OO160_001004"
     CDS             55432..56175
                     /locus_tag="OO160_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5523220.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56205..56744
                     /locus_tag="OO160_001005"
     CDS             56205..56744
                     /locus_tag="OO160_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5523221.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56849..57247
                     /gene="yciA"
                     /locus_tag="OO160_001006"
     CDS             56849..57247
                     /gene="yciA"
                     /locus_tag="OO160_001006"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5523222.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57287..58015)
                     /gene="tonB"
                     /locus_tag="OO160_001007"
     CDS             complement(57287..58015)
                     /gene="tonB"
                     /locus_tag="OO160_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5523223.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58230..58526
                     /locus_tag="OO160_001008"
     CDS             58230..58526
                     /locus_tag="OO160_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5523224.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14055 a        15129 c        14636 g        14709 t
ORIGIN      
        1 ttctggaagc aagccacgca gtgggttcaa cccgatgcca tcgtgagagg cgaggaagtt
       61 aaaccaggtc gttttactgg agggcaagct cagactttgc gcccactggc acagcgcctc
      121 gacgttctgt ttttgcaccg catgtaacac cagcggcggc agcgagaact ggtacaccat
      181 atgcgcttcg tcatcaccgt tgccaaagta agcaatgttg tctttgtgcg gaacattggt
      241 ttcggtaata atcactgtac ctggtgctac gtcatcaata atcgaccgta acagcttgat
      301 aatcagatgt gttttttcca gatggatgca gcttgttccc ggctctttcc acataaagcc
      361 aacggcatcc aggcggacat actcagcacc tttctcaagg taacagagta aaacatccac
      421 catcgccagc aacacttcag ggctacggta attcaggtca atttgatcgt cactaaaggt
      481 ggtccataaa tggcgcgtgg aattatcatc catttgaaac ggcgttaata gcggtaacgc
      541 acgcggaagg gtgacggcac tgagatcggt tttcggatca accgcaataa agaaatcttc
      601 aaaacccgga agctgttgta aatagttttt aaaccattca ctttttgcag acatatggtt
      661 gcagacaaaa tcaaacatta aatgactgca ttcaccgagt tgctgaatat cctgccactc
      721 ccccgcttca ctggcgacct gatgataatc aattaccgaa aatccatcat cagatgacca
      781 tgggtaaaaa ggtaataaat gaacatgtga aaaaatgctt tgcagccatt gatggtagaa
      841 ctgattaaat atgggtagtg gttttaaatc attactgtga aattgatcgg cataggtaat
      901 taacacgaca tcactttcat cccagtgttt tttacgtcgc tgtgtaatta atcgtttcgc
      961 actctcaaga cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc caccgtagat
     1021 ttcgtccatg taatccgtaa ttttctgttt catgttgcct ttccgtggta gcggtcccat
     1081 gaggcgagag actactccgc agaaaataaa ctgtcaattg agcacaaggg ttttagtcac
     1141 gcaggaaatc attttttacc agggaaaaag cgtatttgtg agctgacgca aataatctat
     1201 agggatgtaa ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct catggtgaat
     1261 tcttttaacc tttgaccttc ggcattgcga tgtctttcag gccaccggcg ttctcaacgt
     1321 gggtatatcc catctcgcta aggagctctt ttgcctgccc ggactggcgt ccggcattgc
     1381 aatacacttt cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt
     1441 ccttcagggg aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac
     1501 gaacatcgat ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa
     1561 gtaagccttt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
     1621 cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt
     1681 tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
     1741 ggcgagcaac tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg
     1801 taagaagtac cagcttgcct gctaatttga aaccaagctt caccttttgc ccggcctgtt
     1861 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
     1921 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
     1981 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
     2041 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
     2101 accgaaaaaa atcgacagca ccaccaggat acgcaccagt tttaccggta catcaaaata
     2161 gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataactt
     2221 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
     2281 atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcaac cagttgcgct
     2341 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
     2401 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
     2461 gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
     2521 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat
     2581 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
     2641 tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg
     2701 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
     2761 gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
     2821 cgccaatctc tttcttcatg cgtgccagcg tatcgtccac cagcgtcact tcatgttcca
     2881 gggacttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct
     2941 ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg
     3001 cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcacgcgcc gaagtagaac
     3061 gtacttcaac cagtgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat
     3121 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
     3181 aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta
     3241 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca
     3301 acagcaacat gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg
     3361 ccgaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg
     3421 tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta
     3481 aagagttgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
     3541 cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag
     3601 cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg
     3661 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc
     3721 gtgtgattga gtacggtgaa ctggagcgcg ttggcggtag ccagccgttg caggtgaatg
     3781 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc
     3841 gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc
     3901 gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca
     3961 agctgcctct gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt
     4021 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
     4081 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccacctgaag
     4141 aagccatcgc cgtttcagaa aatacctcgc ttccgacgct gccgctggat ttacgcgagt
     4201 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc
     4261 agaagcgcgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa
     4321 agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat
     4381 tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta
     4441 ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt
     4501 gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg
     4561 tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg
     4621 acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta
     4681 ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc
     4741 ccagttgatt cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg
     4801 cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg
     4861 cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt
     4921 tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg
     4981 tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca
     5041 ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcac tcgccctgca
     5101 ccggttcgaa aataatggcc gcgacctgct tcgcttcgat gtctgattta aacaagcgtt
     5161 cgatggcgtc gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt
     5221 gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca
     5281 gcgccatggt catatatgta cgaccgtgga agccaccgct aaacgcaatc acgccaggtc
     5341 gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga
     5401 agaatgcggt tttagcctgc ccgctcactg gggcaagggt gttaattttc tccgccagcg
     5461 tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct
     5521 gctccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg
     5581 cggcgaaatc gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact
     5641 gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat
     5701 ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa
     5761 acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc
     5821 agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgctcg
     5881 gcgtcaccgc gcagcagttc agcaatcaaa cgcccggcaa gatgggtaca ggtgacgccg
     5941 tggccgctgt aaccctgcat gtaatagatg ttggtatcaa ggcggccgaa ctgcggcatg
     6001 tgcgacaagg tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcaac
     6061 tgagggaagg tttttagtag tttcggtacc acaagacgct caacgtcatc cgggttgcgt
     6121 gcaccataca ctacaccccc gccgtacagc aggcggttgt cggcggtaag acggtagtaa
     6181 tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa
     6241 tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgct
     6301 agttccggct ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc
     6361 gtcacctgac cattggcagt ccgaaccacg gctggcgtgg tgtgctggat ctgcgtcacg
     6421 gcggaaagct catacacgcg cccgccgttc aggcggatgg catcggcttc accaatggcg
     6481 aggtttagcg ggtgaatatg cccaccgctg tgatccagca gcgcaccagt gtagcgatcg
     6541 ctggcgacct cgcggcgaat agcgttgacg tccagcagct ccagctgttt attgccgtag
     6601 cgtacccagt tctctttctg ctcttcgagt gtggcgagtt gtttatcatt catcgccaca
     6661 aacagaccgc cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg
     6721 atctcgccac cctcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt
     6781 ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg
     6841 cttgcgccaa agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc
     6901 agatgcagcg cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg
     6961 attgactcat tcagtgtgtc gaatggtgca tatttattcg cactggcggc gtagtaactg
     7021 ctggtatgtt cggtcatttc aggcctccag gcttatccag atggttttca gttcagtgaa
     7081 tttttcaagg gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa
     7141 cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg
     7201 tcggctcatg cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta
     7261 ctggctgtcg ttggcaagct gcagcgcctg atcttctgat gtgaaacggg tgaccaccag
     7321 caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtccacat ccacgaagat
     7381 ggtcgggccg atggccgctg ccagcccggc gttgcgacca tccagcagca gttgcccctt
     7441 gccttcgcct tcacgaataa aggtgtgtac cgagtcagcg tgagcgctgt cgattaaggt
     7501 gcccatggtg gttgcgggat caagtggatg gcccggctgc cagttttgcg cctgctgttt
     7561 taacagggct aagaattcat cggcgatgct ctcttccagt aacaaacgcg ttccggcgat
     7621 gcgcacctgg ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg ctttttgcag
     7681 atccggacaa tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacacg
     7741 tttcatgttg ctatcacccg cgtctttcag cagttgttta cccgtacgcg tggagccggt
     7801 aaaggcgatg gcatcgatat cgttatgacg cgaaagcgcc tgccccgctt catgaccaaa
     7861 acccgtcacc acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag
     7921 acgaatcgca ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc
     7981 cagcgccggg ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc
     8041 ggcaatcacg ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc
     8101 cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc
     8161 gggaatatca tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag
     8221 tgccagctct tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg
     8281 tttagccgga gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac
     8341 cgcacggtcg atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac
     8401 cggatcaacg gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa
     8461 taagcggttt tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa
     8521 attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg
     8581 cagagatccc cgggctgcat gcccgtttat ttctggcgac attctttatg gattgccggg
     8641 cgcattgcgc ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg
     8701 ctgataattc ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta
     8761 atggcataac tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt
     8821 tcaccttcca gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt
     8881 gtgcccggct ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga
     8941 accagcttca ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg
     9001 acctgcggct catcaggttt ttccggctcg gaaaagaatt ccgagagtga cagaccatac
     9061 accttcagca gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta
     9121 ctgatagcac tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc
     9181 tgctggcgga tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca
     9241 tttccttaaa ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgccaagcgg
     9301 tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt
     9361 gccactgtac gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat
     9421 ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgta
     9481 gagagtttac ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt
     9541 caacctgaac ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc
     9601 gatgttccag caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca
     9661 ccaccagttc ttgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg
     9721 cattaattat cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt
     9781 tttcaccata taggtgcggc tgcacattgc tgggactact aggaagataa atgccatcga
     9841 gtttcggcaa aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg
     9901 gcaagccgcc tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat
     9961 gacccttaag cctgttcctg cacattacga caccgataac cggattgttc attatatttt
    10021 ccatgctcaa tctcacaaaa tggactaaat tatcgccatt actgcgcttt aaatgacctt
    10081 tatgttcaat attttttcaa tctagcagtg gattttcatt tttgcaaact caatttaaca
    10141 tttgacaaac atttggtttg catacagatt cgaatgatgg tcattatatt ttacgctttg
    10201 ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag
    10261 agaactttgt tcagcagtca gaagagaggc ggggtagcgc ctttacgcag gaagtgaaac
    10321 gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg
    10381 gttgcttccg tggaaaacgt atccctgttt caagcctgaa gaagctcgag aaagggtgtt
    10441 atttcccggc ctcggtattt gcaatggata ttctgggcaa cgtagtagaa gaagcaggtc
    10501 tgggtcagga aatgggcgag ccggatcgta cctgtgttcc tgttctcggt tccttaactc
    10561 cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg
    10621 gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctgcgcc
    10681 agcgcggatt gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgccagc
    10741 gcgacgctga aggggatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    10801 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    10861 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    10921 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    10981 tggcactaaa acgcctagtg cgtctgatgg cagaaaagca taagatgcac gccactttta
    11041 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    11101 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    11161 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    11221 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    11281 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggtga ccgtcataat caccgcgtgg
    11341 aatatcacgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    11401 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    11461 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    11521 agaatgatca cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta
    11581 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    11641 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    11701 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    11761 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    11821 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    11881 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    11941 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    12001 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    12061 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    12121 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    12181 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    12241 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    12301 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    12361 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    12421 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    12481 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    12541 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    12601 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    12661 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    12721 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    12781 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    12841 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    12901 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    12961 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    13021 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    13081 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    13141 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    13201 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    13261 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    13321 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    13381 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    13441 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    13501 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    13561 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    13621 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    13681 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    13741 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    13801 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    13861 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    13921 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    13981 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    14041 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    14101 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    14161 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    14221 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    14281 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    14341 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    14401 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    14461 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    14521 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    14581 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    14641 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    14701 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    14761 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    14821 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    14881 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    14941 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    15001 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    15061 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    15121 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    15181 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    15241 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    15301 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    15361 acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc
    15421 tcgcccactg gtgcaatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    15481 agctggcagc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    15541 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    15601 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    15661 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    15721 gttcctgctg accttcgttg gcttcagctt gagctatttc actccacacg cgccgttaca
    15781 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaat ggcctgatcc actgggattt
    15841 tggtgtttcc agcattaacg gtcaaccaat tgccgaacag ttaaaagagc tcttcccggc
    15901 gacgatagaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    15961 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    16021 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacactgt tttgttcact
    16081 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    16141 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    16201 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    16261 tgaagtgatc cgcctgatgc gtatcagcac tattgaagtg tatgaccaga actatgtaaa
    16321 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    16381 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    16441 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    16501 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    16561 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    16621 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    16681 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcgggac
    16741 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    16801 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    16861 ggactgacga cctcggacgt gatgttttaa gccgtttact gagcggagct gcgccaaccg
    16921 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    16981 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    17041 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    17101 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    17161 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    17221 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    17281 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    17341 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    17401 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    17461 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    17521 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    17581 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    17641 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    17701 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    17761 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    17821 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    17881 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    17941 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    18001 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    18061 tgacgaaccg acaaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    18121 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    18181 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    18241 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    18301 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatactctac ccggcgctat
    18361 cccattgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    18421 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    18481 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    18541 taagtaaaac gttccgctac cggaccggtt ggtttcgtcg tcagaccgta gaagcggtaa
    18601 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    18661 cgggtaaatc cacactggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt
    18721 tattgattga cgatcatccg ctgcattttg gcgattattc cttccgtagt cagcgtattc
    18781 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcacaaattc
    18841 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    18901 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    18961 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag
    19021 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc aatgcgttcg cagttgatta
    19081 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    19141 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    19201 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    19261 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    19321 ctgaatcctc cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat
    19381 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    19441 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    19501 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    19561 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    19621 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    19681 gaggcggtgc tggagattat tctgcaggaa ctgaaccgcc ggtcaccctc accctgaaag
    19741 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    19801 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    19861 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    19921 gagagggtta gggtaagggg aaaagcgccc cccctgccgc agcctgctcc ggtcggacct
    19981 ggtaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    20041 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    20101 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc
    20161 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    20221 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    20281 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    20341 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    20401 gctggatggt gactatgtta acgccgttac ccgtgaaagc ttcaaaattg cccacgacat
    20461 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    20521 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    20581 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    20641 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg
    20701 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    20761 agttactatt gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg
    20821 tatctccggt gaagtagtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    20881 cgaactgaaa taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct
    20941 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    21001 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    21061 aaaagccact ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg
    21121 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag
    21181 acgctggccc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc
    21241 cctgaccagc ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg
    21301 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    21361 cttacgccgc tccccggacc tgattcctcc agggaaagta atcatcggtt ggcacaaatc
    21421 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    21481 catctgaatg acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt
    21541 tttattggct ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta
    21601 cgctggttac ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    21661 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt
    21721 tcaactgcag aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc
    21781 cttctcgaaa agttgttaaa cagcacacgg gtcaatgcac agccggataa tcagcttgcg
    21841 gcgttgttat gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca
    21901 tcaaatgaat ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga
    21961 tgggctgacc gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga
    22021 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    22081 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    22141 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    22201 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    22261 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    22321 aaaaggcttt ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca
    22381 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    22441 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    22501 tcagggcaaa aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat
    22561 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    22621 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    22681 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    22741 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    22801 agatctgacc tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga
    22861 cgcccttttc gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga
    22921 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    22981 caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct
    23041 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    23101 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    23161 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag
    23221 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    23281 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    23341 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    23401 agaagcgatg attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt
    23461 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    23521 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    23581 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    23641 cttccagtca tttgctgaaa ttagctctga acccggtcct cactttggcc tcggtctgga
    23701 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    23761 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    23821 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    23881 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    23941 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    24001 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    24061 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    24121 agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    24181 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    24241 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    24301 tataaaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    24361 atttaaccgc ggactccgct gttaaccgga ggatatgcat catgaaaacc gttagggagt
    24421 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    24481 gcgatgcttt gcgcttttct accaccaaaa ccacagaacc tgatcgtaca ttgcagttat
    24541 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    24601 gtctgaccgt cgatgaaagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    24661 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcggttgct cgtgatttat
    24721 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    24781 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac acagggttga
    24841 aagaacacga cgtcattggg taaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    24901 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    24961 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca
    25021 gcggcagtgg caaaaacgag atttttttaa tctgttccgg taccgacatt accgaagagc
    25081 gccgcactca ggagcgactg cgtattctgg caaataccga cagtatcacc ggactgccga
    25141 atcgtaacgc aatgcaggag ttaatcgatc acgctattaa tcaggcagat aacaataaag
    25201 ttggggttgt gtatcttgat ttggataatt ttaaaaaggt caacgacgcc tatgggcatt
    25261 tgtttggtga ccagttatta cgcgacgtgt cattggctat tttaagctgt ctcgaacatg
    25321 accaggtgtt ggcgcgtcca ggtggcgatg agtttctggt actggcatcc aatacctcac
    25381 aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg cttacgcctc ccctttcgta
    25441 ttggtttaat tgaagtttat accagctgtt cagtaggtat ttcgctctct cccgaacatg
    25501 gttcggacag cgcgactatt attcgtcacg ccgacacagc aatgtacaca gcgaaggaag
    25561 gcggacgagg acaattttgc gtttttactc cagaaatgaa tcagcgggta tttgaatatc
    25621 tctggctgga taccaacttg cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc
    25681 acctagcgtg tcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt
    25741 gggttgattc caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct
    25801 ttaggccgtt gggtgattct cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc
    25861 ataaacctgc gagtggcggt aaatatttct gcacgtcagc tcgccgatca aaccattttc
    25921 accgccctga aacaggttct ccaggaactc aattttgaat actgccctat agatgttgaa
    25981 ctgacagaga gttgtctgat tgagaatgat gaactggcac tgtctgttat tcaacaattt
    26041 agccaactag gtgcgcaagt gcatctggat gattttggta ccggctactc ttcactttcg
    26101 caactggcgc gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt
    26161 cacaaacaac ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg
    26221 aatcttcagg tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag
    26281 aacgggatca atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc
    26341 gaacgctggt ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg
    26401 ctatatcccg tcattatata tcgtattcct ggatgataat tagccgttgc tgttaaacac
    26461 ttctctttta gcggcaattt ttgcattatt cattttcccc cttttgctat aaggatgtcc
    26521 ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc
    26581 cttgtggcag gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc
    26641 ttgcgccagg ttgctgaatc tacgccagac atcccgaaga caccgtggga gatttaacaa
    26701 tcgtgccttc aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt
    26761 attataacgt tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg
    26821 tcattgacca gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag
    26881 ttaccattcg ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg
    26941 gctttgcagt ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc
    27001 tgaagcgtga actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta
    27061 tcgaaaatgg cagcagcaat gccctgctcg cccggactct gggggagcag aagaaaaatg
    27121 tcactatcat cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaag
    27181 tcattttgct cggtggcgtg taccagaaaa aaagcgaaag tatggttggc cctttgacac
    27241 gccagtgcat ccagcaggta catttcagca aagcatttat tggtattgat ggctggcaac
    27301 ctgaaactgg attcaccggt cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg
    27361 aaaaagagtg cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat
    27421 actccatcgg tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca
    27481 gcgatctgat gcatcttgag cacagtaaac tcaccgttca cgtcgttgac atttaaccat
    27541 ttcccttctc tacggatgat ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg
    27601 ttgataatta agactattta cctgttatta acactctcaa gatataaaat tattatcagc
    27661 gatataacag gaagtaatta tcacctgcgt gatataaccc tgcgcgcgag cagatttcac
    27721 ggaataattt caccaggctt attcttagct attatagtta tagagagctt acttccgtga
    27781 atcataaatt caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt
    27841 ctggcaatta ctttggcaat gtctctgagt gcctgttcta actggtctaa acgggaccgc
    27901 aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt
    27961 acgttgggta cattaggtgg tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa
    28021 taatcgtaaa taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt
    28081 acgaaataac tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc
    28141 tttttatgtt aaccgcctgc gagttttacc ttcatccctt tcgcttcaag tagtgatttt
    28201 aataaatcac gcttatcgcc ctggatttca ataactccat ctttaactgc tccgccgcag
    28261 ccgcattttt tcttcagttc cgctgcaagt tttgtcagtt cggcatcatc gagatcgata
    28321 ccggtaatca ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc
    28381 gctccatcgc ctttagggcg tactggggcc gctttgggct catcaatacg cccggtttcc
    28441 gttgagtaga ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt
    28501 tgatcgcttt cagtgtctgc gctggatcta ccgattgcgt taccgggcga ccaatcacca
    28561 tataatcaac accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcaccag
    28621 cttcactccc ctgcggacga atgcccggtg taaccagttt gaactcctga ccgaatacct
    28681 gtttaaagcg cacagcttcc tgagcagaac acaccacacc atcaaggtca catttttgcg
    28741 tcagtgctgc cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca
    28801 ggtcgctggc ttccatgctg gtcaacactg tcacagcaat caaaagcggt gcatctttgc
    28861 caaacggaac cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg gaggcatgaa
    28921 cattcaccat ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg
    28981 ggatatcgtg gaatttcagg tcaagaaaga tatcaaaacc acgctgttga agttcgcgca
    29041 caaactgtgg cccaaacaat gtaaacatct ctttaccgac cttcagacga caatcgcgtg
    29101 ggtcgatctt gtcgacaaag gacagcgcgt catcacaatt atgataatca agggcaacaa
    29161 ccacaggaga attcgtaacg gcgcgggaag aagatgaagc agttaacgtc atgaccagac
    29221 cttcttgatg atgggcaccg ggagtggcgc gaaacaggta aacggcgagc attctacccg
    29281 tgaaaataaa aaattaacag ttgcgatttt tcccctagca ggctttttct gctccgtcgt
    29341 tgccggagca caatgggaca tcaataacta gtatgttgta actaaagtcg gattttttta
    29401 aaaattacag gccatcaaga ccgcgaatcg gtttaatagt tgaccaggca cgacaagacg
    29461 gacaatgcca gtagagggtg tatgcggtaa aaccacattt ctggcagcta taacgaggct
    29521 tactacgcac cttctcgcca accatgtcac gcagcaccat cagactctct ttggcgcgcc
    29581 cttcttccgc ttcatttaag tggtaatcca ttagcttatg gaatacacgc atggtcggat
    29641 gacgctgaag ctggcgtgta atatagacct gtgcagcctc actaccgtcg cgcgcttcga
    29701 tgatatcagc aagcatcaat tcagcatcgg caccggtgtt ctcttccacc gcgcgttgca
    29761 ggaattctgc ccattcggca gttttaccca actgctgata gcaggtttgc aacatttcca
    29821 gcgtttcgct gaccagttct ctgtcctggg atatgacgcg ttgcaaactt tcgacggctt
    29881 tggcgtattc tcctttcgcc ataaacacgc gtcccatcat gatggatacg cgggcgctgt
    29941 ttttatctgc cgccgcccct ttttttagca aggtcatggc acgatcgaga tcgtcgctgg
    30001 ccatatgctg cagggctaac tcacagtaga aatgggcaat ttcgacgcgc tgtttatctt
    30061 tacccagctt caccaggcgt tcggcaacat caattgcttt ctgccactca ctggtagcct
    30121 ggtagatttg tagcaactgt tgcagcgcgc caatgcggaa gtcagtttca tcggtcagct
    30181 gattgaacat gtcttccgcg cggtcgtata acccggcggc catgtaatca cgccccagtt
    30241 gttgaatcgc caacagacgc tgttcatagg tcagcgaggc gctttccatt agggtctgat
    30301 ggatgcgaat agcgcgatca acttcgccac gcgaacggaa caggtttccg agcgtaaggt
    30361 gggcttcaac ggtgcctgta tcctctttaa gcatatcgag aaacaggtct accgctttat
    30421 cctgttgatt actaagcagg aagttaaccc ccgctacgta atcacgcgac aagcggttgg
    30481 cttcatcttg cttgttttgt tgcgcacttc tgcggcccat ataccagcca taggcagcgg
    30541 ctacaggcaa aagcagaaac aacaactcca gcatagaaag ttattccttc gccgctgacg
    30601 agtgcggcac tacagccacg tcagtcgcgg gtgaaagctg gttttccagt cgctttattt
    30661 tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca caaatcaacc
    30721 aaccgatagc aaaccccgca gcaaacaata ccgccagcaa tgtggaaata cggtactccc
    30781 cttgcgctaa cagataatta aacgtcacct gttgatcgtt ctgcgcaccc aacgtgaccg
    30841 aaatcacgaa gatcgctaac accagtaaga aaatgagtaa atatttcaca ttacttcccg
    30901 ttatgtggtt agagcgaata aattatggtc aacttaccgc aatagccctt taaagtacca
    30961 ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa ggcctaaatt
    31021 cggaaaatca accagcgtta actttcttgt tctcgttgtg caatttcgcg attttcttcc
    31081 gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc acgttgccac cgccaccagc
    31141 gcccacgaaa tcaacgtagc taccaccaga tcgcgtggcc aatgcatccc gagcagcagg
    31201 cggcttccca tgactcccgt tgcccagacc aacaagatag caatggttaa cgttcgccga
    31261 cgcggccaca gcaaaccaac ggccagcagt gcccaactgg cagcaaacat cgtgtgaccg
    31321 gaaggaaagg caaaccccgt ctctttctgc cagtgtgaac gcaaatattg tggaatattt
    31381 ttctcttcag ccaactgttc ttttactaga tttccgcgtt ctgctcgctt taaagtgtag
    31441 aactcatcaa ccggaatatg atgtgttttt tccaaccaga taacaaaagg tcgtggttcc
    31501 tgtactttgt ctttgatcca ggatttaacg ccttgtccaa caaggattgc cgccgccaga
    31561 atggcaaata atacaaaggc agccttaatg cgaaaacgca gacaccagag aaaccagccg
    31621 aacaaaatca aatgtgtaat gacgccccag ggctgggtga cagtttcagt aacccaaaaa
    31681 gccgctttta gtagccaact ttgttctcca ggttgccaac gccagccaga aatccatacg
    31741 gctactggca tgacaagcaa tagtgcagct cccactgcgg tacgtcgggc aatcgaacgc
    31801 atggcctctc ctttttgata agtcccacaa tcataactga aaatgccagt tccaagaaaa
    31861 attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt gaaccccttc
    31921 tcgttatggc aaaataagcc aatacagaac cagcattatc tggagaattt catgcagctt
    31981 aaacgtgtgg cagaagccaa actgccaacc ccatggggcg atttcctgat ggtgggattt
    32041 gaagaactgg caaccggaca cgatcatgtt gcgctagtct atggcgatat ttccgggcat
    32101 accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct gttcagcttg
    32161 cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc aaattgccga ggaaggccgt
    32221 ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa taaaatccgc
    32281 gcttacgcac tgcaggatca aggttacgat accgtagagg ctaaccacca gttaggcttc
    32341 gccgctgatg agcgcgactt cactctttgc gctgatatgt tcaaactcct tggcgtcaat
    32401 gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa ttctgaccga agcagggatt
    32461 aatattgttg aacgcgtacc attgattgta ggtcgtaacc ccaataacga acattatctc
    32521 gataccaaag ccgagaaaat gggccatttg ctgaacaaat aaccctcttg catcgtgtaa
    32581 ttcatttgct tgccggaagc aaaataaccg gcaagcaaat acttgttact tcaacatatt
    32641 acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag gtcaactccg tcgcggtgtc
    32701 gatacgacaa cggcagggta cgacttcctg gctaccatcc gcgcgcgtaa gcgtcaccgg
    32761 aaccgtcgcg ccaggttgta gattttgcag atcgccaata tcaatcttct cttccccggt
    32821 tagccctaac gttttacgcg tcacgccttg cggaaattcc agcggcagga tgcccatgcc
    32881 aattaaattc gaacggtgaa ttcgttcaaa cgattcggca atcaccacac gaattccaag
    32941 cagacgaggt ccttttgccg cccagtcacg gcttgagcct gatccatact ctttcccggc
    33001 aatcaccgcc agcggcgttt gctcctgctt atagcgcatc gcagcatcat aaatagagac
    33061 tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca acgccaggca ccatttcatt
    33121 acggatgcga atattggcga aggtgccgcg catcatcact tcatggttac cacgccgcga
    33181 accgtaggag ttaaagtctt ttcgctcaac accccgacct tgtagatatc gacccgctgg
    33241 gctgtcgggc ttaatactgc ccgccggaga gatatggtca gtggtgactg aatcccccag
    33301 cattgcgagg atccgcgcac cgtgaatatc ttccactggt gctggtgttg cctgcatttc
    33361 atcaaagaaa ggcgataagc gaatataggt tgagtcctcc tgccaaccgt aggtatcgga
    33421 tcgtgtgacg ttaatttcct tccactctgc tgtgccttca aaaacttctg cgtactcttt
    33481 gcggaacatt tctgtggaga cttgttctac cgcacgggca atttcttgtg ccgatggcca
    33541 gatatctttc agataaaccg gctcgccttt gcgatcatgg ccgataggct cagaagccag
    33601 gttgatattc atatttcccg ccagcgcata ggcaaccacc agcggtggcg aggccagcca
    33661 gttagtttta accagcggat ggatacggcc ttcaaagtta cggttgccgg acagcaccgc
    33721 accgacggtt aaatcgcctt ttttgattgc cgtttcgata ggatcgggca gcggcccaga
    33781 gttaccaata caggtggtac aaccgtatcc cacaaggtta aaccccagtt cgtcgagata
    33841 cggtgtcagt ttcgcttttg ccagataatc agaaacgact ttcgaacccg gtgccaacga
    33901 cgctttgacc cacggttgcc gcttgaggcc aagagttacg gcttttttcg ccagcaagcc
    33961 tgcggccatc agcacacttg ggttagaggt gttggtgcac gaggttatcg cagcaatgac
    34021 cacagcgcca tcaggtaact gatactgatg tccgttcata acataatcga ctggcaggcg
    34081 atctttatgc gtggcattca ctttcagttc gttactggcg gcaaatgctt ttggtacatc
    34141 gggcagtgca acgcgatcct gtgggcgttt tggccctgcc aggctcgctt caacgtcatt
    34201 catatccagt tctaacatac tggtaaaaat cggttcatcg cccgggttac gccacatgcc
    34261 ctgcgctttg gcatattttt cgaccaactc gacctgatct tcgctgcgcc cgcttaaacg
    34321 catgtaatcg agggttactg catcgattgg gaagaagcca caggtggcac catattctgg
    34381 cgacatattg gcaatggtgg cgcgatccgc caacggtagt gaatccagac catcaccata
    34441 aaattcgacg aatttcccca ccacgccatg tttgcgcagc atttgggtaa cagtgagaac
    34501 caggtctgtg gcggtaatac cttcacgtaa ttttccggta agtttaaagc ccactacgtc
    34561 cgggataagc atggaaaccg gctggcctaa catggcggct tctgcttcga tcccacccac
    34621 gccccacccc agcacgccaa ggccgttgat catagtggtg tgcgagtcag taccaacgag
    34681 tgtatccgga taagcaatcc attcaccgtc ctgcaattcg ctccacactg ctttgccgag
    34741 atattcgagg ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact
    34801 gaacgcttgc tttccccatt tcaggaacac ataacgttcg tggttgcgct ccatttccag
    34861 gcgtacgttt tcttcaaatg cctcatcatc accaaaacga tcgacggtca ccgagtggtc
    34921 aatgaccagg tcgaccggtg agagcgggtt aacctttgca gtatcgccac cgaggcgttt
    34981 aaccgcttcg cgcattgccg ccagatcaac aacggcaggt acgccggtaa agtcctgcat
    35041 cagcaccctt gccgggcggt aggcaatttc acggtcagca tgggcatttt tcagccatcc
    35101 cgccagtgcg tggatatcct cttcggtaac tgagttacca tcctgccagc gcagcaggtt
    35161 ttcgagcaaa actttgagtg acttgggtag acgggtgata tcgcccagtg atttagcagc
    35221 aagcggcagg ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgt ccttactggc
    35281 ttctcgtagg gttgacgaca tagctcctcc ttaatgacag ggttgcgaag ccctgattct
    35341 cttcagggtg aatattaaag ataacacaaa catcgcgtaa cgatttgata acaacccaaa
    35401 ttgataaaag aggagaaacc ttgtggcaaa gcaaatgaca accccgccgc agcggggtca
    35461 aggatcagtg agtaatgatc cagaggagtt cggcccagaa gagcatcgac agagaaaacg
    35521 cgcccatcca ggaccaatat ttcatatttg tgttgatcat aggctacacc cttaacggtt
    35581 attattttgt gttgagataa ctcaagctgt tattgctgcg gtgtattaaa agccctttaa
    35641 ttcattaaaa atgggtaata agaatgatga taattattgt ttcggctgat catcttcagc
    35701 aaacagttct ataaaggctt gctgctgcga tgttagttgc cagtcgtctg gaataacgac
    35761 ggaacattcc cgtttcgttt ctggttcatt gtcactatgc tggcataatg gtgacgactc
    35821 cagttgctcc tgaccgacca tagttatccc cacactttcc aaatcagcag tgcgacaacc
    35881 acccagaaaa gtgcagaacc aagaaaaacc gccagccagg ctttacgttt taattcagga
    35941 tcacggcggg gttcctgatt tccagaaggc attgctaacc tcatataatc gccatcactt
    36001 atcagcaaga cgttgaatga ttggtgtaat taatcatacg atgagacaaa tcctaaagaa
    36061 actttagtca aaaatccagc gaatttacga atcgtctcca gtgaataatt caatcttttg
    36121 acctgaaata gatatttgaa aactggaatt tgcgaggcgg gtaattagac actactttac
    36181 aggtaattta ccctataaat atgagggata aaaatattgt gcacggtata gaaataagta
    36241 acaaaagaga atatattccg gcaccttcgc tacataaaag gtgccgaaaa taacgcaaga
    36301 aattattttt ccggcagttt tatatcttta aacatgacct caatttcttc attggagcgc
    36361 aatgcgacag ccgcatcaac gacatcacgc gttaaatgcg gtgcaaaacg ctgaatgaaa
    36421 tcatacatat aactgcgcaa aaaagtacta cggcgaaaac caattttggt tgtactgtgg
    36481 ctgaagatat cgtgagcatc aacgcgcaca aggtcgggat cggcgaccgg atccaccgcc
    36541 atgctggcaa tgacccctac ccccagccct aaccggacgt aagttttaat gacgtcagca
    36601 tccgttgccg tgaaaacgat acgcggcgtt aaccctgcgc ggttaaaggc agtatccagt
    36661 tctgaacgtc cggtaaagcc gaaggtatat gtcaccaacg gatattgcgc cagttcttca
    36721 atggtaatgg cttttttgcc tgccagcggg tgatccggag tgactacaat agcccgattc
    36781 cagtggtagc acggtaacat cactaaatct tcatacagat gcagcgcttc tgtggcgata
    36841 gcgaaatcgg cattgccttt agagacggca tcagcaattt gtgtcggcgg gccctggtgc
    36901 atatgcaaag aaacgcgggg ataacgctca ataaagcctt tgataacgtt tggtaacgcg
    36961 tagcgtgcct gggtatgcgt ggtggcgata tacagcgagc ctttatccgg ccaggtgtgc
    37021 tcaccggcaa ccgattttat ggcatcgact ttcgacagga cttcgcgagc gatacgaatt
    37081 atttcttgtc ctgctggcgt tacctgcgtc aggtgcttgc cgcttcggga aaaaatttga
    37141 atgcctagct cgtcttccag cattctgact tgtttactga tcccgggttg tgatgtgtaa
    37201 agtccttccg ctgttgatga gacattcagg ttatgattga ccacctcaac aatatagcga
    37261 agttgttgta atttcatgtt aaaccatcca cttagacgcc atcagaacat cgttttttcg
    37321 tcacacctgg cgggtgacta atacgattta ataataaaaa aggtgctaac tataaccact
    37381 atatcattta tagccttagt gtataggtgt gaagaaaaaa taatatgcaa taaaaaaggg
    37441 ccgctttcgc gaccctttgt ttataaaaac ctgacagaat taaaggttat ttttttcctt
    37501 caacccattt gccatcaaca taaaatgctg accagccagt cgcctttccg tctttttccg
    37561 aagagacata ttgctgtttg gttttacggc taaagcgaac catggtctta ttaccttccg
    37621 gatcctgctg tggcgcatcg gccagataac gcagtttttc cggcagacgg tcgcggaagc
    37681 gataaagctc ttccaccagt ggcgcacgcg tttcacgcga tttcgggaaa gtgttggcag
    37741 ccaggaacac accggcagca ccgtcacgca gcacgaaata agcatctgat ttttcgcacg
    37801 gcagctcagg taatggcacc ggatcttctt tcggtggtgc cacttcaccg ttacgtaaaa
    37861 tcttacgtgt gtttttacac tcttcgttgg tgcaggccat gtatttaccg aaacgcccca
    37921 ttttcaggtg catttcagag ccacattttt cacactcaac gatcgggccg tcataacctt
    37981 taatgcggaa ttcgccctct tcgatctcgt aaccgtcgca ggttgggtta ttaccacaga
    38041 catgcaactt acgtttcgga tcgatgagat agctgtccat cgccgtgccg catttcgggc
    38101 aacgacgttt tgcgcgcagc gcgttggttt cagcatcttc gccttccagc acgttcagca
    38161 cttcgttttc cggcaccagg ttaatggtgg ttttgcaacg ctctttcggc ggcagcgcat
    38221 agccagaaca gccaaggaat accccggtac tcgctgtgcg aatccccatt ttgcgaccac
    38281 aagtcgggca gtcaatgctg gtcagaacca tctggttcgg gcgcatacca ccctcttccg
    38341 gatctttttc agctttatct aactgctggg tgaaatccga gaagaagtga tccagtacag
    38401 ctttccactc tgcttcgtga tttgccacct ggtcgaggct gttttccatc tgcgcggtaa
    38461 agtcgtagtt cattaactcg cggaaatttt cttcaaggcg atcggtgacg atttcgccca
    38521 ttttttccgc atagaaacga cgattttcta ctcgcacata gccacgatcc tgaatggtcg
    38581 aaatgatcga cgcataggta gacggacgac cgataccacg tttttccagt tctttaacca
    38641 gcgatgcttc actgaaacgg gctggcggct tggtaaagtg ctgggctggt gttagttcaa
    38701 cgagcgtcag agcatcgcct ttatcgactg ccggtaagat acgatcttca tcgcctttac
    38761 gcagtgcagg catcactttt gtccagccat caaagcgcaa aatacgaccg cgtgctttca
    38821 ggcggaaatc gcccgcacca acggtcagcg tcgtggagtc atatttcgct ggggtcatct
    38881 ggcaggcaac gaactgacgc cagattaact ggtacagttt ctgcgcatct gcttccatat
    38941 ccttcagcga ttccgccatc acattgacgt cagaaggacg aatcgcttcg tgcgcttcct
    39001 gtgagttttc tttgctggcg tactgattcg gactttccgg cagatatttc ttaccaaaat
    39061 tatcgctgat ataaccgcga accatattta ccgcgtcctg actcaggtta gtggagtcgg
    39121 tacgcatgta agtgatatag cctgcttcat acaaacgctg cgccatcatc atggtttttt
    39181 tcacgccaaa accaagacgg gtgctggcag cttgttgcag cgtagaggta ataaaaggag
    39241 cgccaggttt actggttgtc ggtttgtctt cacgttccag cacgctgtag cgcgcttttt
    39301 ccagcagact taccgcagcc tgagtttgtt ctttgttgac cggacggaac ggtttgtcat
    39361 tctgatgagt cacctgcaac gccaacgctt caccagatgg cgtagtcgtg ctggcatcga
    39421 cttcccagaa ctcttccggt acgaacgctt taatttcacg ctcacgctct accaccaggc
    39481 ggaccgccac cgactgcaca cgaccggcag acagaccacg agcgatcttt ttccacagca
    39541 gcggcgaaac catatacccc accacgcggt ccataaagcg acgcgcctgc tgggcattaa
    39601 cacgatcaat attcagctca cccggtttgt taaatgcctg gcggatcgcg tttttagtaa
    39661 tttcgttaaa caccactcgg ctatagcgcg catcatcacc accaatcact tcccgcaggt
    39721 gccatgcaat ggcttcccct tcgcggtcaa ggtcggttgc gagatagatg tggtcggctt
    39781 tttcagccag ttgtttcagt tcagagacga ccttctcttt accaggcaac acttcatagt
    39841 gcgcctccca attgtgccac gggtcaaccc ccatacggtt gacgagagcg ccacgttcat
    39901 cctttttagg ctttttagcc gtcttggtgg aggtagagtc ggcactcttt ttggcagctg
    39961 agccactggt cggcaaatcg cggatgtgac cgacgctgga tttcaccacg tagtcacaac
    40021 ccagatactt gttgatcgtt ttggcttttg ccggggactc aacgatgaca agagctttac
    40081 ccatattcac cttacctaat ttgattcttc caggaatgcg tcgcacgttg ataaaccttc
    40141 cactggcaac gagttaccga tatggactcg ctctctgcgg atatcaaccc cttgtcgtga
    40201 ctctgttcac gaaaattcag gtgactgagt taacaggtct tggcctacag gcgctatagc
    40261 acgacggaat tttggtcgaa tgtcaagcaa atctattgcc agattgacga aagcgtcaca
    40321 ctgtacctga taaaatccga tacgcaactt tattagcatg caaaatccaa agttgccagt
    40381 aaggcgctct tgccaccagc gtggtatatt tgtctgcaag ccatgcctgg cgtagactaa
    40441 tgtccttgtt taaggagtaa ataatgaaca aagagacaca acccatcgac cgcgaaacgc
    40501 tgttgaaaga ggccaacaaa atcattcgtg agcatgagga tacgctcgca gggattgaag
    40561 ccaccggcgt cacgcagcgt aatggcgtgc tggtatttac cggcgactac tttttagatg
    40621 aacaaggatt gccaacagcc aaaagcaccg cggtgtttaa tatgttcaaa cacctggccc
    40681 atgtgctttc agaaaaatat catctggttg attaaagcaa aacgcgaggt cttagcctcg
    40741 cgtttgtctt ttacatcaat ggcttttgac cccgctgcca ccagcgtagc aacaatcgat
    40801 cggcgctctc tgccgcgctg ccggtgaatc ggtcaatgag tcgtttacgc tgcatatagc
    40861 gtacattaac cacttcacgg ccttccatca ggctaagaat aacttcatca ctggtgttga
    40921 tttcatcaac caggcctttc tctaccgcct gttgtccgta ccagtgttca cccgttgcca
    40981 cctgttcaat atccagagac ggacgcatac gcttcacaaa atctttaaat aactgatgcg
    41041 tttcgttcag ctcttcgcgg aatttctccc gcccttcttc ggtattttca cccagcaagg
    41101 tcagcgtgcg cttatactgc ccggcggtgt gcagttcgat atcaatatct ttgcttttca
    41161 ggaagcggtt aaagttgggc atttgcgcca ccaccccaat ggaacccaca atagcaaacg
    41221 gtgcggaaac aattttgtcc gccacacagg ccatcatgta accgccgctg gcagcgactt
    41281 tgtctaccgt aacagttaaa ggaatgtttt tatcacgcag acgctgcagc tgcgaagccg
    41341 ccaacccgta accatgcacc atgccaccag ggctttccag acggagcaca acctgatcct
    41401 gcggtttgaa tgctgcgagt accgccgtta tctcttcacg tagcgagttc acttcatggg
    41461 cgtccatgct gcctttaaaa tccagcaccc agacgcgagg tttactgtca gttgccacct
    41521 cgcccagttt ggctttcgct tttgctgctt tcgcttcttg cttgtgcttt ttcttctgcg
    41581 ctttgtgcca ctgtttttgc tgatgtgagt ccatcagcgc cgcggccagt tcttctttca
    41641 tctccttata ctgttcgctg agattgttga cccgtaactc gccacgctgg cgtttattac
    41701 gttgagcaac attgacaata atggcggcaa tcgccgcaat cgctagcaca acggtaacga
    41761 ttttagccaa aaacaaacca tattcagaca acaattccac gagtccccac cttggttaaa
    41821 caacgctttg gctcacagtt tacaacaggc atgttaagcc gtctcgcaga gatgttattg
    41881 cagcgaaagt atgacaaagt atccattttg cggtgttatc gccccaggct gattgaaatg
    41941 cccttctgtt tcaggcataa agccccaaag tcataaagta cactggcagc gcgacagaca
    42001 acgtcgttga ggagtagcca tgcattacca gccaaaacaa gatttactca atgatcgcat
    42061 tatcctggtg acgggagcca gcgatgggat tggtcgtgaa gccgcgatga cgtatgcacg
    42121 ctatggtgcg acagtgattc tgttgggccg taatgaagaa aaattacgtc aggtagccag
    42181 ccacataaac gaagaaactg ggcgtcagcc acagtggttt attctcgatt tgctgacctg
    42241 cacgtccgaa aattgccaac aactggcaca gcgcattgtc gttaattatc cgcgtctgga
    42301 tggtgttttg cataatgccg gattgctcgg cgatgtttgc ccaatgagcg aacaaaatcc
    42361 gcaggtctgg caggacgtca tgcagatcaa cgttaatgcc acctttatgc tcacccaggc
    42421 actgcttcct ttattactca aatcggacgc cggttcactg gtctttactt catcaagcgt
    42481 tggacgtcag ggacgagcca actggggtgc atatgcagcg tcgaaatttg ccaccgaagg
    42541 gatgatgcag gtactggccg atgaatatca gcagcgcctg cgcgtcaact gcattaaccc
    42601 aggcggtacg cgcaccgcaa tgcgcgcaag cgccttcccg accgaagatc cacagaaact
    42661 taaaacaccc gctgatatca tgccgctcta cctctggctg atgggcgatg acagccgccg
    42721 taaaaccggc atgacctttg acgcccaacc gggccgtaaa ccaggaattt cccaatgagt
    42781 gatgaacgct accaacagcg tcagcagcga gtgaaagaaa aagtagatgc tcgtgtggcc
    42841 caggcccagg atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc
    42901 accgcggcat ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg
    42961 cagtttatta aaggcacctg gcctaatggc gaacgcaatc tgctggagcc acatggcgtt
    43021 gagtttcagg tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc
    43081 gccgcctgcc gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat
    43141 atggttttgc ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa
    43201 gtggtgcagg cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc
    43261 tgtcatcggg atattcttga actggcggat acggtaagtg agctgcgccc catcagacat
    43321 gcgtttgatg ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg
    43381 gatatgacgt ccggcatact ttgattaacc gttattacgc ccgccagaac gacgactgcc
    43441 gctcacctga ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg
    43501 gtctttttct acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag
    43561 atagttagtc tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag
    43621 tgggatatca ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca
    43681 cagacgacga acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat
    43741 cccttcgccg ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac
    43801 gccacgactc aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta
    43861 ttcacgttca acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt
    43921 gaacagcagc agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc
    43981 acgcagcttt ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca
    44041 caactcacct tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg
    44101 taccgaaatc agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc
    44161 gccgagttta gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat
    44221 ttcacggcgg gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga
    44281 gcttcctttt ggtgtcgcct tcacaggcgt cgaacaggag aatcaatggc gccgtatagc
    44341 gccattttaa ggccgcgtag tatacagagt tacgcactta taagaaaggc ttcacatcac
    44401 ctacgccttc acgcaccacg accggcgtat catcggtaag atcaataacc gttgttggtt
    44461 tctgcccgag atagccgccg tgaataatca aatctacctg tttttccaga cggtctttaa
    44521 tttcttcggg atcggattcg gtaaattcgc tgcctggcag cattagcgaa gtggaaagca
    44581 tcggttcgcc cagtgcttca agcaacgcct gcgcaatcgg gttagaaggc acacgcatac
    44641 cgatggtttt gcgtttctcc tgcaacagac ggcgtggcac ttccttcgtc cccttcagga
    44701 tgaaggtata gttgcccggc gtgttgtttt tcatcaaacg aaacgcaacg ttatccacga
    44761 atgaataggt cgagagttca gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg
    44821 gcagctggcg aatacgacaa atacgctcca tggcgttttt atcttcaatt ttacagccga
    44881 gcgcatagcc ggaatcagtt ggatagacaa tcaccccgcc tttacgcacg atctccaccg
    44941 cctggttgat cagacgttgc tgcgggttat caggatgaat ataaaaaaac tggctcataa
    45001 ttccctctct gtggtgttct gcggctgttc ccatagctgc cagacgcctt caacgcctgc
    45061 gggcagccag agtttacgac ccagttcgat ccacgggcat ggctgatgaa aatcagatcc
    45121 ttgtgatgcc cataaatgat gctgacgcgc aagtgtggcc agctgggtgc gttcattggg
    45181 cgattgctgg cactgcgcga cttccatcgc gtcgccgtgg tgttcggcaa aatgcgcaac
    45241 caatcttttc agccatttag cactaagatt gtaccgccca ggatgagcta gcaccgcctt
    45301 accgccagaa tgatgaatga catcaatagc ttgttctatt gtacaccact gtggcggaac
    45361 gtatccggtt ttcccgcgcg ccagatactt tttaaagaca tctgccattg aactggcttt
    45421 gccgcactcc acaaggaaac gcgcaaaatg accgcgcgtc actgcgccgc cctgcgccag
    45481 tcgttgtgcg ccttccagcg ccccaggtat ttgcgctttt tcaagtcttt cggcaatttg
    45541 ctgcgcccgc tgattgcgcc gttctgtctg ctgcgcgagg aactcacaca ttagcggatg
    45601 agtaatatca atattcagcc cgacaatatg aatttcatga ttttcccaga ccgtggaaat
    45661 ttccacgccg ggaataagat tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg
    45721 cgcgattgca gctgtggtgt catggtcggt gatcgccagg gtgccgacgc gcatctcgac
    45781 tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg
    45841 caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg
    45901 ttttttgcgc cgacatcata acgggtctgg caaatattct gaaatgagct gttgacaatt
    45961 aatcatcgaa ccagttaact agtacgcaag ttcacgtaaa aagggtatcg acaatgaaag
    46021 caattttcgt actgaaaggt tggtggcgca cttcctgaaa cgggcagtgt attcaccatg
    46081 cgtaaagcaa tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag
    46141 agaataacaa tgcaaacaca aaaaccgact ctcgaactgc taacctgcga aggcgcttat
    46201 cgcgacaacc cgactgcgct ttttcaccag ttgtgtggga atcgtccggc aacgctgctg
    46261 ctggaatccg cagatatcga cagcaaagat gatttaaaaa gcctgctgct gatagacagt
    46321 gcgctgcgca ttacagcatt aggtgacacc gtcacaattc aggcactttc cggcaatggc
    46381 gaagccctgc tggcactact ggataacgcc ctgcctgcgg gtgtggaaag tgaacaatca
    46441 ccaaactgcc gtgtgctgcg cttcccccct gtcagtccac tgctggatga agacgcccgc
    46501 ttatgctccc tttcggtttt tgacgctttc cgtttattgc agaatctgtt gaatgtaccg
    46561 aaggaagaac gagaagcaat gttcttcggc ggcctgttct cttatgacct tgtggcggga
    46621 tttgaagatt taccgcaact gtcagcggaa aataactgcc ctgatttctg tttttatctc
    46681 gctgaaacgc tgatggtgat tgaccatcag aaaaaaagca cccgtattca ggccagcctg
    46741 tttgctccga atgaagaaga aaaacaacgt ctcactgctc gcctgaacga actacgtcag
    46801 caactgaccg aagccgcgcc gccgctgcca gtggtttccg tgccgcatat gcgttgtgaa
    46861 tgtaatcaga gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc
    46921 gctggagaaa ttttccaggt ggtgccatct cgccgtttct ctctgccctg cccgtcaccg
    46981 ctggcggcct attacgtgct gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag
    47041 gataatgatt tcaccctatt tggcgcgtcg ccggaaagct cgctcaagta tgatgccacc
    47101 agccgccaga ttgagatcta cccgattgcc ggaacacgcc cacgcggtcg tcgcgccgat
    47161 ggttcactgg acagagatct cgacagccgt attgaactgg aaatgcgtac tgaccataaa
    47221 gagctgtctg aacatctgat gctggttgat ctcgcccgta atgatctggc acgcatttgc
    47281 acccctggca gccgctacat cgccgatctc accaaagttg accgttattc ctatgtgatg
    47341 cacctcgtct ctcgcgtagt cggcgaactg cgtcacgatc ttgacgccct gcacgcttat
    47401 cgcgcctgta tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc tatgcagtta
    47461 attgccgagg cggaaggtcg tcgccgcggc agctacggcg gcgcggtagg ttatttcacc
    47521 gcgcatggcg atctcgacac ctgcattgtg atccgctcgg cgctggtgga aaacggtatc
    47581 gccaccgtgc aagcgggtgc tggtgtagtc cttgattctg ttccgcagtc ggaagccgac
    47641 gaaacccgta acaaagcccg cgctgtactg cgcgctattg ccaccgcgca tcatgcacag
    47701 gagactttct gatggctgac attctgctgc tcgataatat cgactctttt acgtacaacc
    47761 tggcagatca gttgcgcagc aatggtcata acgtggtgat ttaccgcaac catattccgg
    47821 cgcagacctt aattgaacgc ctggcgacga tgagcaatcc ggtactgatg ctttctcctg
    47881 gccccggtgt gccgagcgaa gccggttgta tgccggaact cctcacccgc ttgcgtggca
    47941 agctgcccat tattggcatt tgcctcggac atcaggcaat tgtcgaagct tacgggggct
    48001 atgtcggtca ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa catgacggtc
    48061 aggcgatgtt tgccggatta acaaacccgc tgccggtggc gcgttatcac tcgctggttg
    48121 gcagtaacat tccggccggt ttaaccatca acgcccattt taatggcatg gtgatggcag
    48181 tacgtcacga tgcggatcgc gtttgtggat tccagttcca tccggaatcc attctcacca
    48241 cccagggcgc tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa ctagagccag
    48301 ccaacacgct gcaaccgatt ctggaaaaac tgtatcaggc gcagacgctt agccaacaag
    48361 aaagccacca gctgttttca gcggtggtgc gtggcgagct gaagccggaa caactggcgg
    48421 cggcgctggt gagcatgaaa attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa
    48481 ccgcgctact ggaaaacgca gcgccgttcc cgcgcccgga ttatctgttt gctgatatcg
    48541 tcggtactgg cggtgacggc agcaacagta tcaatatttc taccgccagt gcgtttgtcg
    48601 ccgcggcctg tgggctgaaa gtggcgaaac acggtaaccg cagcgtctcc agtaaatccg
    48661 gctcgtcgga tctgctggcg gcattcggta ttaatcttga tatgaacgcc aataaatcgc
    48721 gccaggcgct ggacgagtta ggcgtatgtt tcctctttgc gccgaaatat cacaccggat
    48781 tccgccacgc gatgccggtt cgccagcaac tgaaaacccg caccctgttc aatgtgctgg
    48841 ggccattgat taacccggcg catccgccgc tggcgttaat tggtgtttat agtccggaac
    48901 tggtgctgcc gattgccgaa accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc
    48961 acagcggcgg gatggatgaa gtttcattac acgcgccgac aatcgttgcc gagctgcatg
    49021 acggcaaaat taagagctat caattgaccg ctgaagattt tggcctgact ccctaccacc
    49081 aggagcaact ggcaggcgga acacctgaag aaaaccgtga cattttaaca cgcttgttac
    49141 aaggtaaagg cgacgccgcc catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc
    49201 gcctgcatgg ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca
    49261 gtggttccgc ttacgacaga gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg
    49321 ttttagcgaa aatcgtcgca gacaaggcga tttgggtaga aacccgcaaa cagcagcaac
    49381 cgctggccag ttttcagaat gaggttcagc cgagcacacg acatttttat gatgcgctac
    49441 agggtgcacg cacggcgttt attctggagt gtaaaaaagc gtcgccgtca aaaggcgtga
    49501 tccgtgatga tttcgatccg gcacgcattg ccgccattta taaacattac gcttcggcaa
    49561 tttcggtgct gactgatgag aaatattttc aggggagctt tgatttcctc ctcatcgtca
    49621 gccaaatcgc cccgcagccg attttatgta aagacttcat tatcgaccct taccagatct
    49681 atctggcgcg ctattaccag gccgatgcct gcttattaat gctttcagta ctggatgacg
    49741 accaatatcg ccagcttgca gccgtcgctc acagtctgga gatgggggtg ctgaccgaag
    49801 tcagtaatga agaggaactg gagcgcgcca ttgcattagg ggcaaaggtc gttggcatca
    49861 acaaccgcga tctgcgtgat ttgtcgattg atctcaaccg tacccgcgag cttgcgccga
    49921 aactggggca caacgtaacg gtaatcagcg aatccggcat caatacttac gctcaggtgc
    49981 gcgagttaag ccacttcgct aacggttttc tgattggttc ggcgttgatg gcccatgacg
    50041 atttgcacgc cgccgtgcgc cgggtgttgc tgggtgagaa taaagtatgt ggcctgacgc
    50101 gtgggcaaga tgctaaagca gcttatgacg cgggcgcgat ttacggtggg ttgatttttg
    50161 ttgcgacatc accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac
    50221 cgttgcagta tgttggcgtg ttccgcaatc acgatattgc cgatgtggtg gacaaagcta
    50281 aggtgttatc gctggcggca gtgcaactgc atggcaatga agatcagctg tatatcgata
    50341 cgctgcgtga agctctgcca gcacatgttg ccatctggaa agcattaagc gtcggtgaaa
    50401 ccctgcccgc ccgcgagttt cagcacgttg ataaatatgt tttagacaac ggccagggtg
    50461 gaagcgggca acgttttgac tggtcactat taaatggtca atcgcttggc aacgttctgc
    50521 tggcgggggg cttaggcgca gataactgcg tggaagcggc acaaaccggc tgcgccggac
    50581 ttgattttaa ttctgctgta gagtcgcaac cgggcatcaa agacgcacgt cttttggcct
    50641 cggttttcca gacgctgcgc gcatattaag gaaaggaaca atgacaacat tacttaaccc
    50701 ctattttggt gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca
    50761 gctggaagaa gcttttgtca gcgcgcaaaa agatcctgaa tttcaagccc agttcaacga
    50821 cctgctgaaa aactatgccg ggcgtccaac cgcactgacc aaatgccaga acattacagc
    50881 cgggacgaac accacgctgt atctcaagcg tgaagatttg ctgcacggcg gcgcacataa
    50941 aaccaaccag gtgctggggc aggcgttgct ggcgaagcgg atgggtaaaa ccgaaatcat
    51001 cgccgaaacc ggggccggtc agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg
    51061 cctgaaatgc cgtatttata tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt
    51121 tcgtatgcgc ttaatgggtg cggaagtgat cccggtacat agcggttccg ccacgctgaa
    51181 agatgcctgt aatgaggcgc tacgcgactg gtccggcagt tatgaaaccg cgcactatat
    51241 gctgggcacc gcggctggcc cgcatcctta tccgaccatt gtccgtgagt ttcagcggat
    51301 gattggcgaa gaaaccaaag cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt
    51361 tatcgcctgt gttggcggcg gttcgaatgc catcggcatg tttgctgatt tcatcaatga
    51421 aaccaacgtc ggcctgattg gtgtggagcc tggtggtcac ggtatcgaaa ctggcgagca
    51481 cggcgcaccg ctaaaacatg gtcgcgtggg catttatttc ggtatgaaag cgccgatgat
    51541 gcaaaccgaa gacgggcaga ttgaagaatc ttactccatc tccgccggac tggatttccc
    51601 gtctgtcggt ccacaacacg cgtatcttaa cagcactgga cgcgctgatt acgtgtctat
    51661 taccgatgat gaagcccttg aagccttcaa aacgctgtgc ctgcacgaag ggatcatccc
    51721 ggcgctggaa tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga
    51781 taaagagcag ctactggtgg ttaacctttc cggtcgcggc gataaagaca tcttcaccgt
    51841 tcacgatatt ttgaaagcac gaggggaaat ctgatggaac gctacgaatc tctgtttgcc
    51901 cagttgaagg agcgcaaaga aggcgcattc gttcctttcg tcacgctcgg tgatccgggc
    51961 attgagcagt cattgaaaat tatcgatacg ctaattgaag ccggtgctga cgcgctggag
    52021 ttaggtatcc ccttctccga cccactggcg gatggcccga ccattcaaaa cgccactctg
    52081 cgcgcctttg cggcaggtgt gactccggca caatgttttg aaatgctggc actgattcgc
    52141 cagaaacacc cgaccattcc cattggcctg ttgatgtatg ccaatctggt gtttaacaaa
    52201 ggcattgatg agttttatgc ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc
    52261 gatgtgccag ttgaagagtc cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca
    52321 cctatcttca tctgcccgcc aaatgccgat gacgacctgc tgcgccagat agcctcttac
    52381 ggtcgtggtt acacatattt gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc
    52441 gcgttacccc tcaatcatct ggttgcgaag ctgaaagagt acaacgctgc acctccattg
    52501 caggggtttg gtatttccgc cccggatcag gtaaaagcag cgattgatgc aggagctgcg
    52561 ggcgcgattt ctggttcggc cattgttaaa atcatcgagc aacatattaa tgagccagag
    52621 aaaatgctgg cggcactgaa agtttttgta caaccgatga aagcggcgac gcgcagttaa
    52681 tcccacagcc gccagttccg ctggcggcat tttaactttc tttaatgaag ccggaaaaat
    52741 cctaaattca tttaatattt atctttttac cgtttcgctt accccggtcg aacgtcaact
    52801 tacgtcattt ttccgcccaa cagtaatata atcaaacaaa ttaatcccgc aacataacac
    52861 cagtaaaatc aataattttc tctaagtcac atattcctca ggtaattgtt aatatatcca
    52921 gaatgttcct caaaatatat ttccctctat cttctcgttg cgcttaattt gactaattct
    52981 cattagtgac taattttaat gagtgtcgac acacaacact catattaatg aaacaatgca
    53041 acgcaacggg agaaataaca tggccgaaca tcgtggtagt tcaggaaatt tcgccgaaga
    53101 ccgtgagaag gcatccgacg caggccgtaa aggcggtcag catagcggcg gtaattttaa
    53161 aaatgatccg caacgcgcat ctgaagcggg taaaaaaggc ggtcaacaaa gcggtggtaa
    53221 taaatcaggc aaatcctgat tcaccgttaa ttattaatat cagctgataa agataattgc
    53281 atctgcgggc cgtatggctc gcagtgattt cactggagaa aaatatgaat atgaagacca
    53341 ttgaagatgt atttattcaa ctgctttcag atacctacag cgcagaaaaa caattaaccc
    53401 gggcactggc aaaactcgca agagcaacat caaatgaaaa attaagtcag gcttttcatg
    53461 cgcacctcga ggaaactcat ggacagattg aacgaattga tcaagttgtg gaatcagaat
    53521 caaatctgaa aattaagcgc atgaaatgtg tggcaatgga aggtcttatt gaagaagcta
    53581 atgaggtcat cgaaagtacc gagaaaaacg aagtgcgtga tgccgcactg attgccgcag
    53641 cacagaaagt cgagcattat gagattgcca gttacgggac attagcgacg ctggctgaac
    53701 aattaggtta ccgtaaagca gcgaagcttc tgaaagaaac cctggaagaa gaaaaggcca
    53761 ccgacatcaa actgactgat ctggccctta ataacgtaaa taagaaagcc gaaaataaag
    53821 cctgaaatat gaattttaac ttttagtcat tttataaaga ggacattttc atgaatcgta
    53881 ttgaacatta tcatgactgg ttacgtgacg cccacgcaat ggaaaagcaa gccgaatcta
    53941 tgcttgaatc catggccagc cgtatagata attatcctga actacgcgct cgtattgaac
    54001 aacatcttag tgaaaccaaa aaccagattg ttcaactgga aactattctt gatcgtaatg
    54061 acatttcacg ttcagtcatt aaagattcca tgagtaaaat ggctgcgctt gggcagtcaa
    54121 tcggtggtat attcccttct gatgaaatag ttaaaggctc tattggcgga tatgtcttcg
    54181 agcaatttga aatcacctgt tacacctcac tattagcagc agcaaaaaat gccggtgata
    54241 cagtctcaat tccgatcatc gaagcgattt taaatgagga aaagcaaatg gccgactggc
    54301 tgattcagca tattccgcaa acaactgaga aatttttaat tcgttctgaa actgatggcg
    54361 tagaagcgaa gaaataatac cctttatacc atgtccttat tgaccccgta tattacgggg
    54421 tcgtttttgt gcggaattaa aaacgatatc ctgctgagaa cataaacacc cacggatcga
    54481 ggcgtacgct atcgtgttgc tgtgcaccgc ctagcttata attggcggtg gtatcgatat
    54541 ccatgtacca cactgacatg ttaaccaacc agtcacggtt aatcagataa tcaaccccca
    54601 cctgcccggc agctccccag gaatctttca gactgagatc ggaaagcccc gcctctttgc
    54661 catgatcgtt aaatccatta tcaaagaagg tggtgtagtt aataccggcc ccaacgtaag
    54721 gacggaattt gctgctggca tcaccaaaat accactgcgc catcagtgtt ggcggcagat
    54781 ggtgaacggt tgcaatatcg ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg
    54841 ctgccagtaa ttccacacca atgttgtcgg tcgccatata agtaaacgtc aagcccagtt
    54901 gagtgttatt ggtcacgctg aatccaccca gacttcctaa cgtaccacca gcaccttctg
    54961 ttggacgtac ggttgcagaa cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg
    55021 cactgccaga aagaagagtt gttactgcca aagccgccac tgttaacttt ttcatatccg
    55081 ctccgtcgtt atggttataa aaacgtgacg aatataccta cattggagta acaaatgatc
    55141 ctacatagat cacattacat atgataattc accatttatt gatctggatt aatttagaag
    55201 ttgcattgag aatcctggtt aatgtgattt aaatcaattt ttaaatattt tctctacgca
    55261 aatattaact cgatcgccac ttgccgcgct ttgcccacgc ctgaaggcca gcaattacct
    55321 ccgctttaca aagatatgct tttccataca agacctgatg ctgccacccg aggcttaacc
    55381 agggtacaat tgcccgctaa ttaacacctg cataaaactc aaggagagtg catgtctatc
    55441 acggcgcagt ccgtataccg tgacaccgga aatttcttcc gtaatcaatt tatgaccatt
    55501 ctgttggtat cgttgctatg tgcgtttatc acagtggtgt tagggcatgt tttctcaccc
    55561 agtgatgcac agcttgcgca gctcaatgac ggcgtgcccg ttagcggcag tagtgggttg
    55621 ttcgacctgg ttcagaatat gtcaccggaa cagcaacaaa ttttgctgca ggcttcagcg
    55681 gcgtccactt tttcagaatt aatcggtaac gccattctcg ccggaggcgt aatattaatt
    55741 atccagctgg tgtctgcggg tcagagagtc agtgcgctgc gagctattgg tgccagtgcg
    55801 ccgatattgc ccaagttatt tattctgatt tttctgacta cccttttagt acagattggc
    55861 atcatgctgg tggtcgttcc gggaattatc atggccattt tactggccct ggcaccggtg
    55921 atgctggtac aggacaaaat gggcattttt gcctcgatgc gtagcagtat gcggctgact
    55981 tgggcgaata tgcgtctggt ggcacccgca gtactgagct ggttgctggc aaaaacactg
    56041 ttgctgcttt ttgcctcttc ttttgccgca ttaaccccgg aaattggtgc cgtactggcg
    56101 aacaccttga gcaacctgat ttctgccata ttgctcatct atctgttccg cctgtatatg
    56161 ttgattcgcc aataacctta agcatttgat tacggaatcg taaaatgaag cagtttcttg
    56221 attttttacc gctggttgtc tttttcgcgt tttacaagat ttatgacatc tatgcggcta
    56281 ctgcagcgct gatcgtcgcc acggcgattg tgcttatcta tagctgggtt cgctttcgta
    56341 aggttgagaa gatggccctg atcacttttg ttctggtggt cgtcttcggt ggcttgacgc
    56401 tgttcttcca caatgatgag tttattaaat ggaaggttac agtcatttat gctctgtttg
    56461 cgggagccct gttagtcagc caatgggtga tgaaaaagcc gctaattcag cggatgctga
    56521 gtaaagaact cacgctgccg caaccggtat ggtcgaagct aaatctggcc tgggctgttt
    56581 tctttatcct ttgcggtctg gcaaacatct acatcgcatt ctggctgccg caaaatattt
    56641 gggtcaactt taaagtcttt ggcctgaccg cccttacctt aatcttcaca ttgttaagcg
    56701 gtatctatat ctaccgccac atgccgcagg aagataaatc ctaaccagac tgccagtcct
    56761 tcggggctgg cgctgtatct cctctgttct cctcaggaaa atcatagtag catcgcgcct
    56821 gtgattttcc ttttaagtcg gttttaccat gtctacaaca cataacgtcc ctcagggcga
    56881 tcttgtttta cgtactttag ccatgcccgc cgataccaat gccaatggtg acatctttgg
    56941 tggttggtta atgtcacaaa tggatattgg cggcgctatt ctggcaaaag aaattgccca
    57001 cggtcgcgta gtgactgtgc gggttgaagg aatgactttc ttacggccgg ttgcggtcgg
    57061 cgatgtggtg tgctgctatg cacgctgtgt ccagaaaggg acgacatcgg tcagcattaa
    57121 tattgaagtg tgggtgaaaa aagtagcgtc tgaaccaatc gggcaacgct ataaagcgac
    57181 agaagcatta tttaagtatg tagcggttga tcctgaagga aaacctcgcg ccttacctgt
    57241 tgagtaatag tcaaaagcct ccggtcggag gcttttgact ttctgcttac tgaatttcgg
    57301 tggtgccgtt aattttaaac aggatattca ccacaatccc actgcctggc ttacccggct
    57361 cataacgcca tctgcgcatc gcatttttca cctcacgctc aaacatgttc gcaggcttgg
    57421 ctgagaggat ttgtacgtta tccacgcgac catccggcgt gacgtcaaat ttaactttaa
    57481 cctgcccttc aatgcgcaat gcctgtgctc gtgccggata ctgcggctga ttacggctta
    57541 atgcgcgtgg tcctgaagcc acactggtaa ccggcttgct ggttgcagcc gttgccgtac
    57601 ttgatgtggg gcgcgcgggt gccgtatttt caaacggtga tgccggacgc gactctacgg
    57661 gtttgacatc gcgttttggc tgctcctgta cctttttcac cggcttcggt tttggcttag
    57721 gtttcggctt cggcttttca atgaccaccg gtgcttcttt tggcggttcg gggatcggct
    57781 caggttccgg ttctggctct accaccggct ccggtggcgg ctgaacggct tgtggcggtt
    57841 cgagatcagc aggcgcaacc atcgtgacag aaattggttg cgcaggcgca ggtagttcaa
    57901 taacctgatg taccgaggta tagagcagcc ccgccacaac agcaccatga atgcagaccg
    57961 aaagtaacgt cggccagggg aagcggcgag gtaaatcaag ggttattgaa gtcataatca
    58021 tttcagtaga aaaactaggc ctcgatttta aatgcaaata gcaatcatat tcaataaggc
    58081 aagacgatca ggatcagtga aaatgtcatt tttggcgtaa ttacccttac tttatgcatg
    58141 accttaacaa agacatcatc tttccgttgc agatcttcgc cctttcaaat aacgtacttt
    58201 acaactttcc cgaacaagga gttgtgcccg tgttgtatgt tatttacgct caagacaaag
    58261 ctgatagcct cgaaaaacgc ctttccgttc gtccggcaca tttagcacgt ttacagttac
    58321 tgcatgatga aggtcgtttg ttgacagctg gtccaatgcc agcggtagac agtaacgatc
    58381 caggggctgc gggatttact ggctcaaccg ttattgctga atttgaatcg ctggaagcag
    58441 ctcaggcctg ggccgatgct gacccgtacg tagcggcagg agtctatgaa cacgtttcag
    58501 tgaaaccatt taagaaagtg ttctgagta
//
LOCUS       DAJTNP010000015        58032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTNP010000015 DAJTNP010000000
VERSION     DAJTNP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(26..280)
                     /locus_tag="OO160_001009"
     CDS             complement(26..280)
                     /locus_tag="OO160_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5523225.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO160_001010"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO160_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5523226.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO160_001011"
     CDS             1230..2696
                     /locus_tag="OO160_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5523227.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO160_001012"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO160_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5523228.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO160_001013"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO160_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5523229.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO160_001014"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO160_001014"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5523230.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO160_001015"
     CDS             5711..7267
                     /locus_tag="OO160_001015"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5523231.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO160_001016"
     CDS             7349..9163
                     /locus_tag="OO160_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5523232.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO160_001017"
     CDS             9164..10258
                     /locus_tag="OO160_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5523233.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO160_001018"
     CDS             10258..11283
                     /locus_tag="OO160_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5523234.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO160_001019"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO160_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5523235.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO160_001020"
     CDS             complement(12878..13222)
                     /locus_tag="OO160_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523236.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO160_001021"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO160_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5523237.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO160_001022"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO160_001022"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5523238.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO160_001023"
     CDS             15618..17378
                     /locus_tag="OO160_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5523239.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO160_001024"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO160_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5523240.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO160_001025"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO160_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5523241.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO160_001026"
     CDS             19115..19342
                     /locus_tag="OO160_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5523242.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO160_001027"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO160_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5523243.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO160_001028"
     tRNA            21197..21273
                     /locus_tag="OO160_001028"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO160_001029"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO160_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5523244.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO160_001030"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO160_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5523245.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO160_001031"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO160_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5523246.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO160_001032"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO160_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5523247.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO160_001033"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO160_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5523248.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO160_001034"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO160_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5523249.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO160_001035"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO160_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5523250.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO160_001036"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO160_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5523251.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO160_001037"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO160_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5523252.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO160_001038"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO160_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5523253.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO160_001039"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO160_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5523254.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO160_001040"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO160_001040"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5523255.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO160_001041"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO160_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5523256.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO160_001042"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO160_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5523257.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO160_001043"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO160_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5523258.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO160_001044"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO160_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5523259.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO160_001045"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO160_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5523260.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37393..37557
                     /locus_tag="OO160_001046"
     CDS             37393..37557
                     /locus_tag="OO160_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523261.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO160_001047"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO160_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5523262.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38302..39333)
                     /locus_tag="OO160_001048"
     CDS             complement(38302..39333)
                     /locus_tag="OO160_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5523263.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39567..39926)
                     /locus_tag="OO160_001049"
                     /pseudo
     CDS             complement(39567..39926)
                     /locus_tag="OO160_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO160_001050"
     CDS             complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO160_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5523264.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO160_001051"
     CDS             complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO160_001051"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5523265.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO160_001052"
     CDS             complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO160_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5523266.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO160_001053"
     CDS             complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO160_001053"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5523267.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO160_001054"
     CDS             complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO160_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5523268.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46677..49349
                     /gene="rcsD"
                     /locus_tag="OO160_001055"
     CDS             46677..49349
                     /gene="rcsD"
                     /locus_tag="OO160_001055"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5523269.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49366..50016
                     /gene="rcsB"
                     /locus_tag="OO160_001056"
     CDS             49366..50016
                     /gene="rcsB"
                     /locus_tag="OO160_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5523270.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO160_001057"
     CDS             complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO160_001057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5523271.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53340..54116)
                     /locus_tag="OO160_001058"
     CDS             complement(53340..54116)
                     /locus_tag="OO160_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5523272.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54121..55770)
                     /locus_tag="OO160_001059"
     CDS             complement(54121..55770)
                     /locus_tag="OO160_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5523273.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55771..56330)
                     /locus_tag="OO160_001060"
                     /pseudo
     CDS             complement(55771..56330)
                     /locus_tag="OO160_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56334..56492)
                     /locus_tag="OO160_001061"
     CDS             complement(56334..56492)
                     /locus_tag="OO160_001061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523274.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56489..>58032)
                     /locus_tag="OO160_001062"
     CDS             complement(56489..>58032)
                     /locus_tag="OO160_001062"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5523275.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14194 a        15654 c        14260 g        13924 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcagcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attattttta tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ttggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccgacagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcacccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcga ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc actgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcggcgcac tgatgaccat
    13861 ccacgcagcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgacaacc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctaaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccagtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc agcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctagtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgttttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg cccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taataaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgaaggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgttaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaagt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc tagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 ggtttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tagcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg actgttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcaaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg aagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc attacaacgc aatatccgcc acttcttttt gaacgggttg
    38341 cggtttcaac tgcggtttcg gcgtactatc tgctgcttgc ggtttgtcgt atttcagccc
    38401 cagcacttcg ctggtgtact gcaactcgcg ttctgttgcc gctacatcac cgttcagctt
    38461 gcgtccataa gacggaacga tcgctttcaa cgtagcctgc cattgcgggc tggaaacgcg
    38521 atcgccaaat actttttcca gtagattcaa cataatcggc gcggcggttg acgcccctgg
    38581 cgatgccccc agcagcgccg caatggtccc ttgctggtca ctgaccactt cggtacccag
    38641 acgcagtacg ccacctttct cggcatcacg cttgataatc tgcacacgct gccccgcttg
    38701 ccacaaacgc cagtcctctt ttttcgcttg cggatagtac tctttcaacg cttcaaaacg
    38761 atcctcttca ctcaacatca cctgactcac cagatatttc accagatcga aattatccag
    38821 cccgacgtgc atcatcggca tcacgttaga ggtggtggtg gaactcatca gatcccacaa
    38881 tgaaccgttt ttgaggaatt tggttgagaa ggtggcaaat ggcccaaaca gcactacgcg
    38941 tttaccgtcc agaacacggg tatcgatatg cggaaccgac atcggtggtg cgccaacgga
    39001 tgctttaccg taaactttcg ccagatggcg attaaccacg tctggatttt ccgaaacaag
    39061 gaactgtccg cccaccggga aaccggcgta gtctttcgct tccggaatcc ccgattcctg
    39121 taacagcttc agcgccgcac cgcccgcgcc gataaagaca aatttggcgc ggatgttctg
    39181 tgcagtgccg tttttcagat cggcaacggt aacggtccag gtgttatcgt cattacgctt
    39241 tagggcgcgg acttcgctgc tgagttgcag cgagaagtta gatttcttct gtaaggaagc
    39301 aattaactgg cgggtgatct caccatagtt cacatcagtg ccaatttctg ttgcggatcg
    39361 cgcccttcca tcactaacgg tgcccactct ttgatctgcg cgtgatcttc agagtaacgc
    39421 ataccgcgaa acagcgagct ttgttgcaac gcggcgtaac gggcgcgcag gaaattaacg
    39481 ttatcctcgc cccagacaaa gctcatatgc ggaacggtat tgataaatga acgcggagta
    39541 cgcagcacgc cgcgctcgac ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg
    39601 ttaatggcga ctgctttttc aatactgatg ctgccatcgg cgttttgcgg ggtgtagttc
    39661 agttccatca gtgcagaatg cccggttccg gcgttgttct agccgttcga actctcctgc
    39721 gcgcccccct ccaggcgctc caccatggtc atcgaccatt caggctccag ctcgcgtcaa
    39781 taggtcccca acgtggcgct cataatgccg ccgccaatca acagtacatc agtttcctgc
    39841 tcctcggacg ctttcgcttt tgccgccatc gaaacggcat taagccccac ggccatcgag
    39901 aagagcatgg cagtcacttt tttcatcttg ttaatgcctt acttttagtc gctttattgc
    39961 aggtgagatt tgcgcggcat caacggtaac acttaagtaa caacatttaa ataatgttta
    40021 taaattatat tctaatttta gaaacattat aatttcgttg attaattata gggttattag
    40081 aaatgaaggg atttttagca tattgacctg acggcagcag tccgccaggt cagcgtcgta
    40141 atattatgcc gtccgggcaa cggcatcacg cgaagcggca tcgcgctctt cgcccgtcag
    40201 ctcgctaagt tgcccattgc gcatttccag caggcggtcg gcgtggataa agtaatgatc
    40261 atcatgactg atagcgaaaa tagttttacc catctcctgc atcagcggca gcaacacctg
    40321 ataaaactca cgacggaagt gtggatcctg atccaccgcc cattcatcca gcaggataat
    40381 atcgcgttct tctgccagcg ccagcaacag cgccacgcgt tttttctgcc cttttgataa
    40441 cttcaggtta acaatacgcc cgttgcttaa ctcaagctta tgagccattt tcagctgcgc
    40501 cagccacttc tcaaccagtt gcgggttagc gggttgcccc tccggcccca gcagttgatc
    40561 aaacagccag acatcggtaa acactgccga aaacagtttg cgataatctt ccggttgttc
    40621 gccgctgaca ggtttgccat ccagcaagat ttcgccgctt tgtggctgat acaagcccgt
    40681 caacaacatc gccagcgtcg attttccgct accgttaccg ccaatcagaa acagcagctc
    40741 gccacgtttg atggtgagat taatcggacc aacggaaaac gcgttatcct gataagcaaa
    40801 cgtcacgtta cgcagctcca gcgtttgcca gttgggaaac gcctgcgggc gcggaaactc
    40861 agctttgaaa ggcgcgagcg cgaatttgtt cagcttgtta aacgccacct gcgccgtcag
    40921 cagcgtcggc aatgcgccaa ccgccgaaag cagcggcgta cgcaggaata acagcgtcaa
    40981 cgaataggtc gcagcaacgt tggtatcagc ccaaccgaga ctgttcgcca tccagaacac
    41041 caggccgata gcgcccagca tcatgatgtt tgaccagttc acggcactaa gatggaaggt
    41101 gtctgcgcga ataatatgat ggcgatactc ttgcgcatcg gggatgtaga ggttgttaaa
    41161 cacatactcg gcgcgttccc ggttcagagt cagctctttg cgcccttcaa gtacagtttg
    41221 aaaatccgtg tacagcttgt cttcggtttc acgtagggtc gccatatgtt tgtacacccg
    41281 cgccaccagc acaaaaccgc cccagatggt gatcgccatc cagatagccg ttaccagcaa
    41341 cattttgccc gacagcatcc acagatacgc cgatgaaccg atagtgagaa tgatcccctg
    41401 ccccagttcc ggcagacgca caaaagcaat ggtgatattg cgcacatcgc tggttaatcc
    41461 cgccagcaac gaggcgctac cgagttgttc aatgcgctcg acgtgggtat ccagaatccg
    41521 cttgataaat tcactacgca gtcggtaaac gaagtgatgc cccaaagtgg tgagcgccag
    41581 ttgcgatccg agagtgactg ccatcaacag cagcaataat cccagaaact ccggtaacac
    41641 cagcagactg gtatccgccg tttcgataag gcgctgattg ataaaagcaa ttaagccaat
    41701 gcctaatgcc gcactggcga ggcttagcgc catcacactg ataaatggcc agcgatactg
    41761 ccgccagaca agtacaagaa gttccatgca gaaaacccgg acaatgaatt acagcccgca
    41821 gtttaaacat cttcgcgcgc acagcaataa taattcttat ttttattctt ttttacctgc
    41881 ctgacggaat gtcaggttgt agcggcagtc ggtggtgagt ggatgaaacc ccgctttcaa
    41941 cggttgaata ccgtgataaa acagccgcga ttcaccgccc cataccacca catcgccatg
    42001 ttccaacaac aaacgtttga gcggatcatt acgtttcagg ccaccaaatt gaaaaatcgc
    42061 gggtaagccc agagaaacag aaacaattgg cgcgcgcaga tccggttcgt ctttatcctg
    42121 atgcagcgac agtttcgcgc caggagcgta gcggttgata agacaagcat ctggctggaa
    42181 atctggatag cccgccgccg tagccgcacg ttgacataaa tcatgaaagc tttgtggcat
    42241 agcgggccac ggtttatttg tttgcggatc aatgggcgaa tagagataac cttgccgatg
    42301 ggtcgtccag cccagatgcc cacagttggt catcgccacc gacatggtat atccgcctgg
    42361 agcgaccatc tggcgaaacg gcgactggct ggcaacgtca ttaatatcgc ggattagttg
    42421 ctccgcagca ttaaaagcaa aacgccgtaa aattaccgca ccagccgcca gtggctcttg
    42481 ccacggttca gcatcggcaa acagatccaa cattacctct cctcattttc agcttcgcgg
    42541 cgcagcagtt gcgctttacg cgacacgccc cagcggtaac cggaaagtgt gccatcacca
    42601 cggaccaccc gatgacaggg tattacgata gccagcttgt tggcggcaca ggcgctggca
    42661 acggcccgta ccgctttcgg tttgccgatg gcgttagcca gttgctgata actgacggtt
    42721 tcaccacaag gtatcgtgcg cagtgcctgc cagacttgtt gctgaaaagc agtgccgcga
    42781 atatccagcg gtaacgtcag cggcgtatcg cgttgattga ggctggcgat cacttcacgc
    42841 acatgttgct gaaacgtcag atcggcaggc gcgttgtcgg cagcgggaaa catctgctgc
    42901 aactcgctga ttaatgtcgc gtcatcatcg cccagcaata tcgcgcaaat cccccgctcg
    42961 ctttctgcca ccaggcaacg acccagctca caatcagcca gcgcgtaacg caccgccaga
    43021 ttttcgccac cgtgacggaa ttgtttagcc gtcatgccca gcgtttcatc ggctttgcgg
    43081 tagtaactgc tgctgtcggg gaatccggcg ttaagaatag acgtcgtcac gctctcccct
    43141 ttcgccagcg attcgcgcaa acggcgagcg cgccaggcct gttgccaggc tttaggcgtc
    43201 attccggtag tagctttaaa caaccgatgt agatgaaatg gactcatcgc cacctggtcg
    43261 gctaaggctt ccagcgttac aggcgtttcc tgttccagca gtcgacacgc gtgggtgatt
    43321 ttatccaacc gatgttgccg gggattggct ttgtctggct gacaacgttt gcaggggcga
    43381 aatccggcgg cgagtgcctc gctggcattt gcgtagaagg agacattttc ccgcaaggca
    43441 tgtctggcgc ggcaagaagg acggcaaaag atacccgtgg tgcgcacggc gaaaacgaat
    43501 tcgccgtcgg cattcgggtc gcgggctaag acagattgcc agcgttgatc gtcagttaag
    43561 catgtggcgt ttttcataat cagctccctg gttaaggata gcctttagtc tgcccggtca
    43621 ccatcacgca aaaaccaaca atcttgcgct ttaatttttt tcgctgacaa ggaagctttt
    43681 aaactgtggt gacatccagg ttttaaagct atcgccttct ttggtgatca tataaaccgc
    43741 cagcccttcc cggcgaacaa cctctttggc tttctccggc ccgagtacca tcaagccagt
    43801 atcccaggca tcggcttcca gcgccgtcgg agcaatcacc gtcacggata ccagattgtg
    43861 ttcgatggga cgcccggttt gcggatcgat aacatgggaa agacgtttgc cgtccagttc
    43921 gtaatagtta cggtagctgc cagaggtgct aatcccatga ccgttgatat ccaccacagc
    43981 ctgaaccgcg ttttctttat cggttggttt ttgaatcgct acccgccacg gctggccttc
    44041 accgttcata ccacggctgt tcagcgcgcc gcccaccgac accagatagc gggaaatccc
    44101 ttcctgctcc atcaagcgtg ccaggtgatc cgccgcataa ccttcaccga cggtggagag
    44161 atcgacatat aaatccggca ggtctttttg cagatattgc tgatgcgact gattgatgac
    44221 cgtcaggtgc tgtaagccgg ttttggcttt catcgcatcg atctgttcct ggctcggaat
    44281 ttgaaccggc tgttgttccg ggccaaagcc ccacagattc accagcggcc cgacggtaat
    44341 atccatcgca ccatcggtcc ttgccccaat gcgcagcgag gtggtgacga tatcggccat
    44401 cgcttcactt accggccacg gcgacaaact ttgcgagtcg ttaaagcgca tcagcgcgga
    44461 atctttttta taggtcgaaa gcagctgatc gtcggcgtcc agctgggtct gaatcttttc
    44521 tttaagttcg gcactgcgtt tggcatcaat gcccgggatg ctggcacgcc agaaggtacc
    44581 catggttttg ccttcaagaa cggtaacttc agtagcgtgg gttttggcgg gttgtggttt
    44641 ttgatcgcaa ccaacaaaga agagcgcggc aaccagcagt gccacgcggg taaagcttat
    44701 ttccatacgt gattatcctc atgcgaacgg tcgcaagagt acaccaaaaa actgtgtttg
    44761 tacgctgaaa acaatgaaaa aagggcccgc aggccctttg ttcgatatcc atcgagatta
    44821 gaactggtaa accagaccca gagctacgat gttatcagtg ttgatgccag cgtcacgagt
    44881 gaactggttg tcgtccagca ggttgatttt gtagtcaacg taggtggaca tgtttttgtt
    44941 gaagtagtag gtcgcaccaa catcaacata tttcaggata tcttcgtcgt cgtagttacg
    45001 accattgatg acacccaggt ttttaccttt agactgcagg taagccaggg acggacgcag
    45061 accgaagtcg aactggtact gagcaacagc ttcgaagttc tgtgctttgt tcgcccaacc
    45121 caagttgcct acgcgagttg cgttgtaggt ctgggtgtac tgagcagcta ggtagatgtt
    45181 gttcgcgtcg tatttcagac caccggtgta ggtttcagcg cggtcgccat taccgatgta
    45241 acgctcatca taacggttca gaccgaagtt ctggtcatca gtacgtttgg agctggaaac
    45301 tgcagcaccg ataccgaagc cttcgtaatc ataagtaata gaaccgccaa cgccgtcacc
    45361 gttctgacgc agagcttcac gaccgttgtt ggtcatgcct tcaccttccg ggctaccgtt
    45421 tttgccctga tactgaacag cgaagttcag accgtcaacc agaccaaaga agtcggtgct
    45481 acggtaggtt gcgaagccgt taccacgctg ctgcatgaag ttgtcagaac cgtaagtgtc
    45541 accaccgaat tctggcagta cgtcggtcca ggaagttacg tcgtaaacta cgccgtagtt
    45601 acgaccgtag tcgaaagaac ctacatcctg gaatttcaga cctgcgaatg ccacacgggt
    45661 ccaggagttg ttttcgtttt cagcgctgtt gccctggatc tgatattccc actggccgta
    45721 accggtcagc tggtcagtaa cctgagtttc acctttgaag ccaagacgca tgtaggtctg
    45781 gtcgccatct acagacttgt cgtcagagaa atagtgcagg ccgtctactt taccgtacag
    45841 atctaatttg ttgccgtctt tgttgtaaac ttcagcagcg tttgctgcgc ctgctaccag
    45901 cagagctggg accaggaggg acagtacttt aactttcatg ttattaaccc tctgttatat
    45961 gcctttattt gcttttttat gccactgcat actgattaac cctcattaat cagtcggcaa
    46021 gtccattctc cccaaaaatg cagaataatc caacacgaat atgatactaa aacttttaag
    46081 atgtttcatt tatcgctata gatgtttcaa aatgtaaata caagggaact ttttaagatt
    46141 attgcggaat ggcgaaataa gcacctaaca tcaagcaata ataattcaag gttaaaatca
    46201 ataacttatt cttaagtatt tgacagcact gaatgtcaaa acaaaacctt cactcgcaac
    46261 tagaataact cccgctatca tcattaactt tatttattac cgtcattcat ttctgaatgt
    46321 ctgtttaccc ctatttcaac cggatgcctc gcattcggtt tttttaccct tctttacaca
    46381 cttttcatta ttctgtgcta ccacagaaaa actataacgc ttgttaacta tttcacaaat
    46441 aattaacatc agcataattt ccagcaatct ttgtttattt gcaattaatt tcgttgggct
    46501 ttttgtaggt tatttgtaca gcaaaatggc gcttgtacat ctatttcccc ccatacagga
    46561 tgataaatat cacgggagaa tagagaatca tcaatcaggt aagagtctgg aatttcacac
    46621 tgtacccttt atactgccct atcacttcgc gaagttttaa caggtcataa acacgaatgc
    46681 gtcagaaaga gacaacggcc acgacccgct tttcactcct accggggagc attacccgct
    46741 tctttttact gttgatcatt gtgttactgg tgacgatggg ggtaatggta caaagtgccg
    46801 ttaacgcttg gctgaaagat aaaagttacc agattgtcga cattacccac gcaatccaaa
    46861 agcgcgtcga tacctggcgt tacgtgacca ggcagatcta cgacaacatt gccgcgacga
    46921 cctccccctc ctccggcgaa ggtttacaag agacgcgcct gaaacaggat gtctactatc
    46981 tggagaaacc acgccgcaaa acggaagcgt taatctttgg ctctcacgac aactcaacgc
    47041 ttgagatgac tcagcggatg tccacttatc tggatacatt gtggggtgca gaaaatgtac
    47101 cgtggtcgat gtattacctg aatggtcagg ataacagtct ggtgctgatc tcaaccctac
    47161 cccttaaaga tctcacctcc ggatttaaag aatcgaccgt cagtgacatt gttgattcac
    47221 gtcgtgcaga aatgttacaa caggccaacg ccctcgatga acgcgaaagt ttttctaaca
    47281 tgcgccgcct agcctggcag aacggtcatt actttacctt gcgtaccacg ttcaaccagc
    47341 cgggacatct ggcaacggtc gtggcttttg atctgccgat taatgatttg atcccaccgg
    47401 gtatgccgct ggacagtttc cgccttgagc cagacgcgac ggcaacggga aacaatgata
    47461 atgagaaaga agggacggat agcgtcagta tccactttaa cagtacgaag attgaaatct
    47521 cctcggcact caactctacc gatatgcgcc tggtctggca ggttccttat ggcaccttat
    47581 tgctggatac gttgcaaaac attctgctgc cactgctgct gaacatcggt ttgctggcgc
    47641 tggcgttatt tggctatacc acattccgcc atttctccag ccgcagtaca gaaagcgtcc
    47701 ccagcacggc ggtcaataac gaattgcgca ttttacgggc aatcaatgaa gagatagtct
    47761 cactgttgcc gctcggcctg ctggttcacg atcaggaatc gaaccgcact gtcataagta
    47821 acaaaattgc cgatcatttg ctgccgcatc tgaatctgca aaacatcacc accatggcgg
    47881 aacagcatca ggggattatt caggcgacga tcaataacga gctgtatgag atccgcatgt
    47941 tccgcagcca ggtcgcgccg cgcacacaaa ttttcattat tcgcgatcag gatcgcgaag
    48001 tgctggtaaa caagaaactc aagcaggcgc agcgtctgta tgagaaaaac cagcaggggc
    48061 ggatgacctt tatgaaaaac attggcgatg cgctgaaaga acccgcacag tccctggcgg
    48121 agagtgcggc taaactcaac gccccggaaa gcaaacaact ggcgaatcag gcagatgtgc
    48181 tggtgcggct ggtcgaagaa atacagttag cgaacatgct tgcggatgat agctggaaaa
    48241 gtgagacggt gctgttctcc gtgcaggatt taattgatga agttgtgcct tcagtgttgc
    48301 ctgccatcaa gcgtaaaggt ctgcaactgc tgattaacaa tcatctgaaa gcacacgata
    48361 tgcgccgcgg cgatcgcgat gccttacgac gtattttgct gctactgatg caatatgccg
    48421 tgacctcaac gcaattggga aaaatcaccc ttgaggttga tcaggatgag tcctctgaag
    48481 accgcctgac attccgcatt ctggacacgg gagaaggcgt aagtattcat gaaatggata
    48541 atttgcactt cccgtttatc aaccagaccc aaaacgatcg ttatggaaag gcggacccgc
    48601 tggcattctg gctgagcgat caactggcac gtaaactggg cggtcattta aacatcaaaa
    48661 cgcgggatgg gcttggtaca cgctactctg tgcatatcaa aatgctcgcc gctgacccgg
    48721 aagttgaaga ggaagaagag cgtttactgg atgatgtctg cgtaatggtg gatgttactt
    48781 cggcagaaat tcggaatatt gtcactcgcc tgttagaaaa ttggggtgca acctgtatca
    48841 cacccgatga aagattaatt agtcaagatt atgatatctt tttaacggat aatccgtcta
    48901 atcttactgc ctctggcttg cttttaagcg atgatgagtc tggcgtacga gaaattgggc
    48961 ctggtcaatt gtgcgtcaac ttcaatatga gcaacgctat gcaggaagcg gtcttacaat
    49021 taattgaagt gcaactggca caggaagagg tgacagaatc gcctctgggc ggagatgaaa
    49081 atgcgcaact ccatgccagc ggctattatg cgctctttgt agacacagta ccggatgatg
    49141 ttaagaggct gtatactgaa gcagcaacca gtgactttgc tgcgttagcc caaacggctc
    49201 atcgtcttaa aggcgtattt gccatgctaa atctggtacc cggcaagcag ttatgtgaaa
    49261 cgctggaaca tctgattcgt gagaaggatg ttccaggaat agaaaaatac atcagcgaca
    49321 ttgacagtta tgtcaagagc ttgctgtagc aaggtagcct attacatgaa caatatgaac
    49381 gtaattattg ccgatgacca tccgatagtc ttgttcggta ttcgcaaatc acttgagcaa
    49441 attgagtggg tgaatgttgt cggcgaattt gaagactcta cagcactgat caacaacctg
    49501 ccgaaactgg atgcgcatgt gttgattacc gatctctcca tgcctggcga taagtacggc
    49561 gatggcatta ccttaatcaa gtacatcaag cgccatttcc caagcctgtc gatcattgtt
    49621 ctgactatga acaacaaccc ggcgattctt agtgcggtat tggatctgga tatcgaaggg
    49681 atcgtgctga aacaaggtgc accgaccgat ctgccgaaag ctctcgccgc gctgcagaaa
    49741 gggaagaaat ttaccccgga aagcgtttct cgcctgttgg aaaaaatcag tgctggtggt
    49801 tacggtgaca agcgtctctc gccaaaagag agtgaagttc tgcgcctgtt tgcggaaggc
    49861 ttcctagtga ccgagatcgc taaaaagctg aaccgcagta ttaaaaccat cagtagccag
    49921 aagaaatctg cgatgatgaa gctgggcgtc gagaacgata tcgccctgct gaattatctc
    49981 tcttctgtga ccttaagtcc ggcagataaa gactaatcat ctgtaggcca gataagacgc
    50041 gttagtgtct tatctggcat ttgcaccgat tgccggatgc ggcgtaaacg ccttatccgg
    50101 cctacgattc ccattatttc aacagattac attaaagtag gccagataag acgcgtcagt
    50161 gtcttatctg gcatttgcac tgaatgccgg atgcggcgta aacgccttat ccgtcctacg
    50221 aatcccgcga tttcctgacc ctctcggcat ataccgtcag cgtctgtttt atcacatcca
    50281 gcgttaccgg cttcgagagg cagctgtcca taccggactc cagacaccgc tgcttctctt
    50341 cagccaacgc attagcagtt actccgatta ccggcaacgt cagtcccaac tgacgaatgc
    50401 gttgcgtcaa gcggtaacca tccatatttg gcatgttgac gtcgctaagc acgatatcaa
    50461 tatgattctt actaagtaca ttaagcgcat cgacgccatc attcgcggtt ttacattgat
    50521 agcccaacga tcccaactga tctgccagca gacgtcggtt aatcggatga tcatccacga
    50581 ccagaatcat catatcgtcg ttatcgctga ccgctttgtc cgtcgacggc agagcgttag
    50641 caggatcgtc gctctccatc tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat
    50701 gcggagcagc cacactgtgt acccactccc ctggcgcttt ctccagcgga ataccaatat
    50761 ggcgacgaca gaaggtcact accgctctgc cctgccattt tttactcact acctcgtcag
    50821 tgattaacac atcttcggga gtcggttcct gcccttcgta tgttgtaacg acgatgccgc
    50881 tgcgctgcaa actggtttcc aggaactgac agagcgacgc attgcggacc gccagccagc
    50941 agcgtttacc actcaaccct tccacgcctt ttttctgcgg gtactgagcg ccgtacaacg
    51001 gaatacgcac ggtaaactgg ctgcccattc ccggttctga atctaccgag atatcgccgt
    51061 ccatcatgct gatcagtttt tcacaaatcg ccagacccag accggtcccc tggaagtttc
    51121 gctgtacgcc cgttccaacc tggaagaagg gatcaaacaa gcgcaccact tctttcgccg
    51181 gaatccccac gccggtatcg cgaacacgga tagagagata atcgccatca gcgcgaacat
    51241 gcaaaactat acagccggta tcggtgaatt ttatggcgtt actcaacagg ttggagatga
    51301 cctgctgtaa acgcatcggg tcgccattta aggccactgg cacatccggt tcaataaagc
    51361 agtacaagcc taactgcttg cgtaccacca gcggtaaata gttggcggtg atgtggttca
    51421 tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat tcaatcttcg
    51481 agaaatcgag aatatcgctg ataattttca acaacaggct ggaagagttg ttcattgccg
    51541 tcaccagacg atcgacgcct ttcggtaact ctttggtttg caacagatcc aggttaccga
    51601 taatgccata cagcggcgtt cgcagctcat gactgacggt ggcaaggaac atcgattttg
    51661 actggctcgc ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc
    51721 gcgaagaaac atccaccagc acacaaatgg ccacgttttc attacgatag cgcgaatgga
    51781 cgaagctgat ttgcagattg gtattgttgc tggtcaggac atcaacaaaa ttgacctgct
    51841 gcccacagat aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata ttgagatagg
    51901 tatgcgccag ttcgttactt aaaatattga cgccatcagc ggtacgcaaa atgcagatac
    51961 ccactggcgc ggaggcgaca atcttgcgat tgaactgctc atgttcttcc agtcgcaggg
    52021 cgtcgctttc cgccggaatg aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg
    52081 cagctccggc aagcacattc agcaaaattg cgttaaggat caacatgcga atgcgttcca
    52141 gcaccttatc aaccggcacc gaatacacga tgcttagcga tgagggtggc agatttttct
    52201 tcagcaccag ctcccggaac ccttccgtat agccaaacca ggagcgttcc tgcatccagc
    52261 gaggatcgcc cttaatttta ctttctggtc cggtaagcga aatcagggta tgaccatttt
    52321 catcaagaat ggtaaccccc atcggcaacg tacccggtaa gaaaaagttc tccatccgga
    52381 tggtctgctc gacacccaaa agcgcctgca accggttcgc cagataaact ggcgtcaacg
    52441 cgtaaaaata cccgacgcaa gggcgcggac cttcgctgat ccagtagagg ttactgccgc
    52501 tatcatcttg tggtgcattt cgatatttat tgatgcgttc atgcaacgct ttcaacgcgg
    52561 tatcgcgttc cactggcata tcacgcagac cgaaattggc catgcagagg ttatcactgc
    52621 cgattaaaaa tacccggttg agatcgtaag ccgcagaaaa attatcgcgc cagtagcgca
    52681 taaaccacgc caatgactcc agagaacctc gccaggtgtt actcattgcg gaacaatcgg
    52741 agtcggcaaa cagcggttca aacgcaggca catccgcctg cgtttctcgt ccacgcgggg
    52801 aaagcacacc gttttctgcc gataagcgat tttcggcgat gtacttaagc tctttcatca
    52861 catcagaggt gcgttgaata aagcgctgag cctgatcgga actcagatta aattcctgac
    52921 gaatttccga ttctcgctga tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa
    52981 tcaacagcca gagcactaac gccaatgctc tgaacatgta gcgcgaggct ttcagggttg
    53041 tacgaaaaga agcaaggtat ttcaaagggg agaagctccg cctcaggtga ccgatggagt
    53101 gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc tgtcaccatt gcaataatgc
    53161 gtatcgctta gaaaccattg ccttaaccag gaaagccgga gtagatggac agttctcagt
    53221 acgaaaaata agaaatgacc aatctgccga cgcttgttaa tggttatatc ctggcgtcgg
    53281 cagatcgccc agcagtctta tctgaaaaaa gagagagcca ggttgtgtga agggatggat
    53341 caccagtcaa aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat
    53401 gtttcctgtt tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtcgtc
    53461 aattccgttt cgctgcgccc accatagtag ttgatataca cctgatagcg cccgtgaact
    53521 ggtgctggca tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca
    53581 ccactgtttt tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca
    53641 tggagatcga gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga
    53701 atagttcctg tacccggcgt tgagtaaaac tgcatttttt gccggctttg cccatccggg
    53761 ctgatgacct gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca
    53821 tcggattcaa ttctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact
    53881 ttacccgccg cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca
    53941 tctgccatgt tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg
    54001 ccctctgccg gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca
    54061 ctgtgagcga caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca
    54121 ttagtccatt aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg
    54181 ataaccatca aaggccaagt gcagatactc atgggttaaa tccagccgat cctgaagcgt
    54241 aaagaagtta cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac
    54301 taaacgacag accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg
    54361 ccgccactgc ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt
    54421 tgagcgtgga gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg
    54481 gtcgtaacgc tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc
    54541 ggtagcgcga ctgccatgat aatgaaccgg atcgcctgca tagatgagct cctgcgtcca
    54601 ggcggtcatc gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct
    54661 gctgtcgggg atggtcaggc aatcaccttc ccgattggcg ttttgctgta aaaaagtacg
    54721 tatcgccaca gtcatggctt tcgctgcctc aggtggcgta cttttcgcct cgcgatccag
    54781 aactctggca acgtactctt cacgatcgag atgcgactgc aatttcagtt tgcccttttc
    54841 gctcagtaag gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tagtaaacgt
    54901 cacacgatag cgcccattaa gtacgctggg ttttactgcc gtcgtactct tttccgctgt
    54961 gattttcttc agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac
    55021 ctgcgtcggc actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt
    55081 gctggtgcca ctgccagtaa cccacagcgg tgtaccatcg gttaaccagc cagcaaaacc
    55141 accctgacgg atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg
    55201 actaccgagc atggatgcga caccgatttt cgcctcgtcc agcaccacat ccagtaagac
    55261 ttcctgagct ttgttttgcg caggtaacgt cgctaatgaa tcaagcagcg atttaaccgt
    55321 taccgaggtt tctggcttca gcgttgtaag agacgctaac cacgcaggtg cctgacgctg
    55381 ttgccagtat tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag
    55441 cccacaggaa cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata
    55501 aacctcttcc ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc
    55561 cagccaggcg taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg
    55621 taatgctgag actttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc
    55681 accgcgcagc accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc
    55741 caatagccag acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact
    55801 ccctgccgcg acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag
    55861 aacgaattgc cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag
    55921 ttctttcacc ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa
    55981 ttgcccctgc tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc
    56041 aacgtcagct cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg
    56101 ctcgctggta agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt
    56161 caccggctgc agagtaaagc tcatctcttc ttcaccgggg ataagccgat acaactggcg
    56221 ttcaacggtc accggaatgt tactttgttg agccgttttt gccggttcac gccagcggac
    56281 ctgcacattt gcggcgataa ttcgtcacca aaagagagaa tgtccggcac accctaacca
    56341 acccaacgcc agtcttcgcc ccctccagtt aacttatgtt tagcccatgc gcccgcaggc
    56401 gcaggcaaaa caactggagg cattgtcgcc atatatttcg ccagccagtt catggccagc
    56461 gcgcgctcaa tagtggattg ctcagcggtc aaaccattaa aatagcggcc gtctgggtag
    56521 cgtcaccacc gctattgagc agcacaaccg tgtgcgccag cggctgattg ctgtttgccg
    56581 ctttatccca cgcatattgc tgaacctgtg gcatcaccgt ggacttcaac tgcgcttttt
    56641 tcagcaacgt catggttaag acgtttgcca ccgcatctgc cagtggagat tccggtgtat
    56701 cgaggatcag gctgtcattg atatcgcgga tgtcttcctc agagaaatct tcatctttag
    56761 ttccgcgccg ggcgatagct tcatcccccc ctttcaacaa cgttttgcac ggcagattca
    56821 tctcctgtgc ccacgccagc accagcgccc gatgcaataa cggcatatta tccgcctgct
    56881 cggcataact gtcgagcata tgctgccagt attccggttg ttgcgttacg ccgagcgcct
    56941 ggctggcctg ccagtcggcg taccatgccc atgccgtaag gaaggcgtca ccattgccat
    57001 cttcacccca ccaggtaaag cgcgctccgg gccccgccag ttgcatcagc cgcagacggt
    57061 tatcctgaat catctgacga atgtcgttag cggcggcact ttgatgatcg gcaagcgaac
    57121 gccaggcgag actgagcggg atcagacggc taccggtgtt gatcacccca ccccacggtt
    57181 catcgactaa cgcatcaaga ttgttgcgga aaatctcctg cggcgtttca ctactttgta
    57241 gccggatatt actcgcctgc tcgggcaaca tcagcgcgtt ctcgccaccg ccgagcatga
    57301 catttttctg ctgttcaacg ggccagctgt tgtccacaaa agacagtttt gtgctaatgc
    57361 tgtcctgcac ttgcccattt tgttgcagtt ctgcacttaa caagccagat tgctgaatat
    57421 tctgcgtcag cgaaatataa ttcgcccctt tgtgcagcgt cagcgtctgg cgcatctcag
    57481 cgcctgcaaa tttagtcacc agcgccaccg gttcgttatc ctgctgactg aagataaaca
    57541 gtcctgccgc cggtttgtcg cccacgcgat acaccgttgg catactccac ttcatgtaga
    57601 gatttttttc cgaacgcaga taagcacgcc cctgcccgac cagcccgtcg ccgttcatcc
    57661 cacgcgcggt gatacgccag cgggttaacg aatcaggcat caggaacgtg aaatacgctt
    57721 tgccttgttt atcggttgtg agtgacggca tccatgccgc ggtatccacc tcttcacgcc
    57781 gtggacgttc aagcattttt actcgccgct cgctgcggtt agtcgcgcca ggcgcaaccg
    57841 gctcgctgga gagcgcctgg tcatagctga taaacgacag actggagctg gtacgcacat
    57901 tgttacgccc cagcggatag aaaaatttgc cgatattcgg cgcgatttct ggttgcagcg
    57961 cgtagatcat ttcatcgacc acgcctaccg ttagctgcgc agaaacaggt ttacctttca
    58021 gcgacgaggt ta
//
LOCUS       DAJTNP010000016        55417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTNP010000016 DAJTNP010000000
VERSION     DAJTNP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO160_001063"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO160_001063"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5523276.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO160_001064"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO160_001064"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5523277.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO160_001065"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO160_001065"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5523278.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO160_001066"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO160_001066"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5523279.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO160_001067"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO160_001067"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5523280.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO160_001068"
     CDS             7826..9559
                     /locus_tag="OO160_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5523281.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO160_001069"
     CDS             9708..10559
                     /locus_tag="OO160_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5523282.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO160_001070"
     CDS             complement(10546..10887)
                     /locus_tag="OO160_001070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5523283.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO160_001071"
     CDS             complement(10889..11767)
                     /locus_tag="OO160_001071"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5523284.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO160_001072"
     CDS             complement(11733..14030)
                     /locus_tag="OO160_001072"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5523285.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO160_001073"
     CDS             complement(14081..14401)
                     /locus_tag="OO160_001073"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5523286.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO160_001074"
     CDS             complement(14416..15495)
                     /locus_tag="OO160_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5523287.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO160_001075"
     CDS             15804..16907
                     /locus_tag="OO160_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5523288.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO160_001076"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO160_001076"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5523289.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO160_001077"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO160_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO160_001078"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO160_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO160_001079"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO160_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO160_001080"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO160_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5523290.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO160_001081"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO160_001081"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5523291.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO160_001082"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO160_001082"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5523292.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO160_001083"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO160_001083"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5523293.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO160_001084"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO160_001084"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5523294.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO160_001085"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO160_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5523295.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO160_001086"
     CDS             30120..30728
                     /locus_tag="OO160_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5523296.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO160_001087"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO160_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5523297.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO160_001088"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO160_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5523298.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO160_001089"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO160_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5523299.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO160_001090"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO160_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5523300.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO160_001091"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO160_001091"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5523301.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO160_001092"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO160_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5523302.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO160_001093"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO160_001093"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5523303.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO160_001094"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO160_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5523304.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO160_001095"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO160_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5523305.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO160_001096"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO160_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO160_001097"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO160_001097"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5523306.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO160_001098"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO160_001098"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5523307.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO160_001099"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO160_001099"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5523308.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO160_001100"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO160_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5523309.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO160_001101"
     CDS             complement(44843..45142)
                     /locus_tag="OO160_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5523310.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO160_001102"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO160_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO160_001103"
     CDS             45895..46494
                     /locus_tag="OO160_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5523311.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO160_001104"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO160_001104"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5523312.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO160_001105"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO160_001105"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5523313.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO160_001106"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO160_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5523314.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO160_001107"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO160_001107"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5523315.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO160_001108"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO160_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5523316.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO160_001109"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO160_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5523317.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO160_001110"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO160_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5523318.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO160_001111"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO160_001111"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5523319.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO160_001112"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO160_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5523320.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13492 a        15018 c        14163 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcctcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaatacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgg
//
LOCUS       DAJTNP010000017        53003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTNP010000017 DAJTNP010000000
VERSION     DAJTNP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(78..1859)
                     /gene="gcl"
                     /locus_tag="OO160_001113"
     CDS             complement(78..1859)
                     /gene="gcl"
                     /locus_tag="OO160_001113"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5523321.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(1949..2764)
                     /gene="allR"
                     /locus_tag="OO160_001114"
     CDS             complement(1949..2764)
                     /gene="allR"
                     /locus_tag="OO160_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5523322.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2842..3324)
                     /gene="allA"
                     /locus_tag="OO160_001115"
     CDS             complement(2842..3324)
                     /gene="allA"
                     /locus_tag="OO160_001115"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5523323.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3554..4480
                     /gene="allS"
                     /locus_tag="OO160_001116"
     CDS             3554..4480
                     /gene="allS"
                     /locus_tag="OO160_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5523324.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            4549..5643
                     /gene="mnmH"
                     /locus_tag="OO160_001117"
     CDS             4549..5643
                     /gene="mnmH"
                     /locus_tag="OO160_001117"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5523325.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(6356..8771)
                     /locus_tag="OO160_001118"
                     /pseudo
     CDS             complement(6356..8771)
                     /locus_tag="OO160_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(8768..9454)
                     /locus_tag="OO160_001119"
     CDS             complement(8768..9454)
                     /locus_tag="OO160_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5523326.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9425..10047
                     /gene="tesA"
                     /locus_tag="OO160_001120"
                     /pseudo
     CDS             9425..10047
                     /gene="tesA"
                     /locus_tag="OO160_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10037..10846
                     /gene="ybbO"
                     /locus_tag="OO160_001121"
     CDS             10037..10846
                     /gene="ybbO"
                     /locus_tag="OO160_001121"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5523327.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            10907..11761
                     /gene="cnoX"
                     /locus_tag="OO160_001122"
     CDS             10907..11761
                     /gene="cnoX"
                     /locus_tag="OO160_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5523328.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(11824..12603)
                     /gene="fetB"
                     /locus_tag="OO160_001123"
     CDS             complement(11824..12603)
                     /gene="fetB"
                     /locus_tag="OO160_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5523329.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12590..13267)
                     /gene="fetA"
                     /locus_tag="OO160_001124"
     CDS             complement(12590..13267)
                     /gene="fetA"
                     /locus_tag="OO160_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5523330.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13413..14330
                     /locus_tag="OO160_001125"
     CDS             13413..14330
                     /locus_tag="OO160_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5523331.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            14327..14785
                     /locus_tag="OO160_001126"
     CDS             14327..14785
                     /locus_tag="OO160_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5523332.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(14786..15193)
                     /gene="cueR"
                     /locus_tag="OO160_001127"
     CDS             complement(14786..15193)
                     /gene="cueR"
                     /locus_tag="OO160_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5523333.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(15318..16610)
                     /locus_tag="OO160_001128"
     CDS             complement(15318..16610)
                     /locus_tag="OO160_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5523334.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(16613..17545)
                     /gene="glsA"
                     /locus_tag="OO160_001129"
     CDS             complement(16613..17545)
                     /gene="glsA"
                     /locus_tag="OO160_001129"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5523335.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            17807..20311
                     /gene="copA"
                     /locus_tag="OO160_001130"
     CDS             17807..20311
                     /gene="copA"
                     /locus_tag="OO160_001130"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5523336.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(20526..20867)
                     /locus_tag="OO160_001131"
     CDS             complement(20526..20867)
                     /locus_tag="OO160_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5523337.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            21005..21799
                     /locus_tag="OO160_001132"
     CDS             21005..21799
                     /locus_tag="OO160_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5523338.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            22003..22482
                     /gene="ybaK"
                     /locus_tag="OO160_001133"
     CDS             22003..22482
                     /gene="ybaK"
                     /locus_tag="OO160_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5523339.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(22519..24171)
                     /gene="ushA"
                     /locus_tag="OO160_001134"
     CDS             complement(22519..24171)
                     /gene="ushA"
                     /locus_tag="OO160_001134"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5523340.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            24389..25609
                     /gene="fsr"
                     /locus_tag="OO160_001135"
     CDS             24389..25609
                     /gene="fsr"
                     /locus_tag="OO160_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5523341.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            25847..27523
                     /locus_tag="OO160_001136"
     CDS             25847..27523
                     /locus_tag="OO160_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5523342.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27656..28960)
                     /gene="gsk"
                     /locus_tag="OO160_001137"
     CDS             complement(27656..28960)
                     /gene="gsk"
                     /locus_tag="OO160_001137"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5523343.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            29112..30071
                     /gene="aes"
                     /locus_tag="OO160_001138"
                     /pseudo
     CDS             29112..30071
                     /gene="aes"
                     /locus_tag="OO160_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            complement(30068..31030)
                     /gene="hemH"
                     /locus_tag="OO160_001139"
     CDS             complement(30068..31030)
                     /gene="hemH"
                     /locus_tag="OO160_001139"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5523344.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(31162..31806)
                     /gene="adk"
                     /locus_tag="OO160_001140"
     CDS             complement(31162..31806)
                     /gene="adk"
                     /locus_tag="OO160_001140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5523345.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            complement(31987..33861)
                     /gene="htpG"
                     /locus_tag="OO160_001141"
     CDS             complement(31987..33861)
                     /gene="htpG"
                     /locus_tag="OO160_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5523346.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(33971..34576)
                     /gene="recR"
                     /locus_tag="OO160_001142"
     CDS             complement(33971..34576)
                     /gene="recR"
                     /locus_tag="OO160_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5523347.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(34576..34905)
                     /locus_tag="OO160_001143"
     CDS             complement(34576..34905)
                     /locus_tag="OO160_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5523348.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(34958..36883)
                     /gene="dnaX"
                     /locus_tag="OO160_001144"
     CDS             complement(34958..36883)
                     /gene="dnaX"
                     /locus_tag="OO160_001144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5523349.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    complement(35561..35625)
                     /gene="dnaX"
                     /locus_tag="OO160_001144"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(37012..37563)
                     /gene="apt"
                     /locus_tag="OO160_001145"
     CDS             complement(37012..37563)
                     /gene="apt"
                     /locus_tag="OO160_001145"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5523350.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(37716..38093)
                     /locus_tag="OO160_001146"
     CDS             complement(37716..38093)
                     /locus_tag="OO160_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5523351.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            38163..38690
                     /gene="priC"
                     /locus_tag="OO160_001147"
     CDS             38163..38690
                     /gene="priC"
                     /locus_tag="OO160_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5523352.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38704..38865
                     /gene="rsmS"
                     /locus_tag="OO160_001148"
     CDS             38704..38865
                     /gene="rsmS"
                     /locus_tag="OO160_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5523353.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(39077..42439)
                     /gene="mscK"
                     /locus_tag="OO160_001149"
     CDS             complement(39077..42439)
                     /gene="mscK"
                     /locus_tag="OO160_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5523354.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(42567..43214)
                     /gene="acrR"
                     /locus_tag="OO160_001150"
     CDS             complement(42567..43214)
                     /gene="acrR"
                     /locus_tag="OO160_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5523355.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            43356..44549
                     /gene="acrA"
                     /locus_tag="OO160_001151"
     CDS             43356..44549
                     /gene="acrA"
                     /locus_tag="OO160_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5523356.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            44572..47721
                     /locus_tag="OO160_001152"
     CDS             44572..47721
                     /locus_tag="OO160_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5523357.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            48267..48641
                     /gene="tomB"
                     /locus_tag="OO160_001153"
     CDS             48267..48641
                     /gene="tomB"
                     /locus_tag="OO160_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5523358.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            48667..48885
                     /gene="hha"
                     /locus_tag="OO160_001154"
     CDS             48667..48885
                     /gene="hha"
                     /locus_tag="OO160_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5523359.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            49057..49608
                     /gene="maa"
                     /locus_tag="OO160_001155"
                     /pseudo
     CDS             49057..49608
                     /gene="maa"
                     /locus_tag="OO160_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            49724..50194
                     /locus_tag="OO160_001156"
     CDS             49724..50194
                     /locus_tag="OO160_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5523360.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            50358..51908
                     /gene="pdeB"
                     /locus_tag="OO160_001157"
     CDS             50358..51908
                     /gene="pdeB"
                     /locus_tag="OO160_001157"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5523361.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(51950..52303)
                     /locus_tag="OO160_001158"
     CDS             complement(51950..52303)
                     /locus_tag="OO160_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5523362.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(52423..52519)
                     /gene="ffs"
                     /locus_tag="OO160_001159"
     ncRNA           complement(52423..52519)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO160_001159"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            52682..52993
                     /locus_tag="OO160_001160"
     CDS             52682..52993
                     /locus_tag="OO160_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5523363.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT        12622 a        13952 c        13648 g        12781 t
ORIGIN      
        1 tcaaaacggg cgagaaaatc atattcacca aataacatcg ataaattagc agagaaacgt
       61 aacatcattt atctccctta ttcatagtgc atgaagcagg tttcagtcgg tgcatccgct
      121 gcgttatcgg cgatatcttc aaattccatg acgttatcca gttcgctgcc catcgaaata
      181 ttggtcacac gctcgagaat aacttccacg actaccggta cccgatattg cgccattaag
      241 gctttcgcct gttcaaaggc tggcgcaata tcttccggtt tgaagacccg aatagcttta
      301 caacctaaac cttccgctac ttttacgtgg tcaacaccgt agccattcac ttcactggag
      361 ttgatattct cgaaagcgag ttgcacgcag tagtccatgt caaaagcgcg ttgtgactga
      421 cgaatcaggc cgagataggc gttattcacc agcacatgga tgtacggaat gttgaactgc
      481 gcgccaacgg ctaactcttc aatcaggaac tggaagtcaa agtcgccaga aatcgccacc
      541 acattgcgtt tcggatcagc ggcacaaccc cccagcgccg ccggaatcgt ccagcctaac
      601 ggaccagcct gaccacagtt gatccagtgg cggtctttaa agacatgcag catttgtgcc
      661 gcagcgattt gtgacagacc aatagtggtg acataacaaa catcgcgacc aaaggctttg
      721 ttcatctctt catacacgcg ctgcggtttc accggcacgt tgtcgaagtg ggttttgcgc
      781 agcaaagtgc gtttacgctg ctggcagtcg gcgacccatt ctttacgaca cggcagacga
      841 cccgcttttt gcatctcctg cgccacttca accagcagtg tcagcgccgc tttagcatca
      901 gagacaatgc cgagatccgg acacagcacg cgaccaattt gcgtcggctc aatatcaata
      961 tgaacgattt tgcgcccttc ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa
     1021 cggttaccga taccaaacac catgtcggac gccagcaacg ttgcgttacc gtaacgatgc
     1081 gcggtttgca gaccgaccat cccggccatc agttcatgat cgtccgggat acagccccag
     1141 cccatcagcg ttgggatcac cggaacgctg gtcagttcag caaactgttg taacagtgca
     1201 gctgcgtcag cattgattac cccgcccccg gcaacaatca ccggacgttc ggcctggatt
     1261 aacatttcta cagctttttc gatctgcata cggctggcag caggtttgta gaccggcagc
     1321 ggttcgtaca tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc
     1381 accagtaccg gacccggacg accagaacgc atcaggtgaa atgcctgttg cagcacgcga
     1441 ggcaccagcg ccgcttcacg aactgtaacc gccattttgc tgaccggttt agcaattgct
     1501 tcaatatcta cggcctgaaa atcttcttta tgcagacggg cgcgcggtgc ctggccggta
     1561 atgcacagaa taggaatgga atcagcagaa gcggaataga gcgcggtgat catgtccgtg
     1621 cccgcaggac cggaagtccc cagacatacg ccgatattcc ctgccgttgc gcgggtataa
     1681 ccttccgcca tgtgcgaaac accttccaca tgacgcgcca gaatgtgacg aataccgccg
     1741 tgcttacgca tcgctgagta gaacggatta attgcagctc ccggaacgcc gaaggcggtg
     1801 gtgatacctt ctttctccag cacatacatt gccgcgtcaa cggctctcat ttttgccatt
     1861 ttattcctac ctctattagt tggaaaaatt ttccaacttt caaattttgt caaaaaaacc
     1921 cgctccgcag agcgggatgc gacagacact atggatgtgc tttcagtccc aacgccgtgc
     1981 tgatatcgcg ggcggtgtct ctgaccagct caccctgact gacaaaacga tcttctgtca
     2041 gtcttgatga aggcccggag atggagatag cggcaacaac tctaccgaca tcatcgtaaa
     2101 ttgctgaagc tatgcaattc agacctacaa catgctcttc tttatctacg gtatagccca
     2161 gttcacgcgc ttgttccagg tccttcagca aggtgggcat atccacaagc gtagttggcg
     2221 taaactgctg caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc gccagcggat
     2281 aaagcagcgc tttgcccgca ccggaagcat gcagcggcag acgactgccc agtggcgcac
     2341 acatcctgac catcgattta cactctaact gaccaattaa taccgcttca ttgccgttac
     2401 ggatcgcgac attgaccgtt tcgccggaaa gtaacatcag gcggcgcata aacggcccgg
     2461 cgacggagag gacatcgcgg ttatggatgt acgccgcacc gacgttaaag acacctaatc
     2521 ctatatgcca ccagcctaat tgactgtcct gatagacaaa atccgctgct tgtaaaacct
     2581 tcagcaagcg aaaggtcgtg gagagcggca aatccagatt gagagaaata tcgctaaccg
     2641 acgaacttcc cccacttttt tccagatatt gcagaatcgc aattccccgc tctaacgcct
     2701 gtgcgccctt ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt ctaacttccg
     2761 tcatacctac gttaactccg acctacaagt gagcataaac aatgaacaat tagtttatct
     2821 tatgcaagtc ggttgcaggc gtcacgcaaa acagagttcc tgttcaggaa tactttcaac
     2881 atcacagttg tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc
     2941 gaaaagtggg tgatgccaga cgttacgatg gtaattcacc ccctgttcac cgttggtgat
     3001 aaacgcccgc agcgttgaca ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa
     3061 cacttcacct ttcattggga taaaggcctg agtgcccagc ggatgacgtt cgagttcgtg
     3121 aatggtcagc ggcagattcg ccggttgcgc gcggttaatg ctgataagcg tacggtcttg
     3181 ctcaagaatc tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat tgttaatatg
     3241 gaaaaaatcc cgttgctgcg tttcgattac gtcgccataa gcactaaagg cttcctgact
     3301 taacggtaat acctgaagtt tcatcttttt gtccttgtaa cataattccg ttcaaccacc
     3361 gccgaccggt cagcttggtt tcaataacag gcaatctaaa accttaagag aacaacatta
     3421 atacaaaagt tggaatgttt ttctaaaact agatttagtc cacaaattga acaggattat
     3481 catgtaaaaa tactgataaa tcagaggata agaaaatcac gcaacttgcg gatttaaaag
     3541 gggatacctg aagatgttcg atccagaaac cttgcggact ttcattgcgg ttgctgaaac
     3601 aggaagtttt tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga tcagttatcg
     3661 cattaaactt ctggaagaga ataccggagt agcgctgttt ttccgtacga ctcgtagcgt
     3721 aacgttgaca gcggctggcg agcatctact ttcccaggcc agagactggc tgagctggct
     3781 ggaaagtatg cccagcgagc tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat
     3841 tgtcatcaac aacctgctct ataaccccca ggccgtcgcc cagttgctgg cgtggctgaa
     3901 tgagcgttac ccctttaccc agtttcacat ctcccgacaa atctatatgg gcgtctggga
     3961 ctcgctattg tacgaaggtt tttcgctggc tatcggggtc acgggaactg aggcgctggc
     4021 aaataccttt agtcttgatc ccttaggatc ggtgcaatgg cgatttgtca tggcggcgga
     4081 tcatccgctg gcgaacgttg aagagccgct aacagaagcg cagttgcgtc gctttccggc
     4141 ggtcaatatt gaagacagcg ccggcacctt aaccaaacgc gtcgcctggc gattgccggg
     4201 gcaaaaagag attattgttc ccgatatgga aacgaaaatc gccgcccatc tggcgggtgt
     4261 tggcattggt tttttgccaa aatcactttg ccagtcaatg atcgataatc aacaactggt
     4321 cagccgggta atcccaacga tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt
     4381 tggcagctgc aaagcggtag aagatattgt gaccttgttt acccaacaca ggccggaaat
     4441 cagcggattt ttagaaattt tcggcacccc acgcagttaa aaatcaagtt tccttgcgcg
     4501 ttggcgcgta aaatagcgcg caagctgatt tgaggttcac ttttaactat gcaagagaga
     4561 cacacggaac aggactatcg tgccctgctg attgctgata cgcccattat tgatgttcgc
     4621 gcccctatcg agtttgagca aggcgcaatg cccgccgcta tcaatctgcc gttaatggat
     4681 aacgatgaac gcgcctccgt tggcacctgc tataaacagc aaggctcaga cgcagcgctg
     4741 gcgctggggc ataaactggt ggcgggtgaa attcgtcagc agcgtatgta cgcctggcgg
     4801 gcagcgtgcc tgcaaaatcc gcaaggtatt ctctgctgcg cccgtggcgg tcaacgttca
     4861 catattgtgc aacgctggct gcatgaagcg gggattgatt atccgctggt ggaaggcggt
     4921 tataaggcac tgcgccagac cgcgattcag gcgaccattg aactggcaca aaaaccgata
     4981 gtgctgattg gcggttgtac cggcagcggt aaaacgctat tagtgcaaca acagccaaac
     5041 ggtgttgatc tggaagggct ggcgcgtcat cgcggttcgg cgtttggtcg cacgttacaa
     5101 ccacaactta gccaggcgag ttttgaaaac ctgctggctg ccgaaatgct aaaaaccgac
     5161 gcccgtcagg atttgcgcct gtgggtgctg gaagatgaaa gccggatgat cggttcgaat
     5221 cacctgccgg aatgtctgcg cgagcgaatg actcaggcgg cgattgcggt ggtagaagat
     5281 ccgtttgaga tccgtcttga acgcctgaat gaagagtatt tcttgcgtat gcatcatgat
     5341 tttacccacg cttatggcga cgaacagggc tggcaggaat attgcgaata cctgcatcac
     5401 ggactttcgg cgattaagcg tcggctgggg ttacagcgct ataacgaact cgctgcccag
     5461 ctggatacgg cgctgacaac gcaactcacc accggcagca ccgacggtca tctggcctgg
     5521 ctggtgccgt tacttaaaga atattacgac ccaatgtatc gctatcagct tgagaaaaaa
     5581 gcggaaaaag ttgtctttcg cggtgagtgg gcggaagtgg cggtatgggt taaggcgcag
     5641 tgatgggcgg tattctgaaa cggcgattgt cgtgagaaca aaatggttgc accagaatac
     5701 agatataaaa aaaccgacat gacagtcggt ttttttattt tttccgccca agacttggtg
     5761 gttccgggca gaaattaaac agtcctcatc tagcaagtga attataagtc aggatgccta
     5821 catattcaaa cagctatgtt ggcgatacaa tcccgacgct agtagtgata ctgcctcaag
     5881 ggattaccgc agtgcggaaa agtactttta agtcctggtc aggatacagg tggtgctgat
     5941 tctggtgctc taatttggag agagacagtc ctcatctagc acatgaagaa aatcatagtt
     6001 ataggcaacg taagggtaag ctgtgattgt ctttctccct taagggagca acggatgaaa
     6061 acttttctcg ataaagttct catcgttctt aaggcgctaa tcgcgctgct ggaactgatc
     6121 cgtcagttta ttgaataact gaggaaacgt agctaaggcc gaaggtttac ctccttcggc
     6181 ctttaaaaaa accgacatga cagttggttt catatttatt gccaaaatgc acactgttca
     6241 tgccagatgc ggcgtaaacg ccttatccgg cctacacaat cttgcaaaat caatatattg
     6301 cacgctcttc gtaggcctga taagcgtagc gcatcaggca atctaacacc tgtcatcacc
     6361 ccacaaactg cctgaacagc gccttaccct taaccagtcg cgcacccagc cagccgccga
     6421 aaagcgacag cagcagcgca ccgctgcacg gcagaacaat ccacaatcgc cagtctggct
     6481 cccacgggaa atcaaacact ttcgcctgca acactgccag tgccgtttcc gcaccaattg
     6541 cggccaccag gccggaaaca aacccaagca tggcgaacat cacaccacaa tgtcgtgcgc
     6601 agcagttttt tccccgcacc gagtgtgcgc cataccacca gctcctgatg acgctggcgc
     6661 attcccacct gcacctgtgc cagcaacagc aacataccgc aggcggtgac cagcaccacc
     6721 atcacctcca gcgcccgact tacctgctcc agcacctgac cgacctgttt taaaatcgcg
     6781 ccaatgtcta acaggctaat ggtcgggaac tggcggttga gttgcgtcaa catgccgttg
     6841 ccattctccc agcggaaact ggtaagccag ctctgcggtt gcccgtctaa tgccccttca
     6901 gggaaaataa aatagaaatt aggccgcaga ctttcccagt ccactttgcg caggctggtc
     6961 actttagcgc ggaactcctg ggtatcgccc ataaaagtca cggtatcacc gagggcaacg
     7021 tttaagcgtt tcgccagccc ctcttccatc gacacttcat cggcttttgg cggccagtta
     7081 ccggcgacaa tcggattatg atcgggccgc gtattttgcc aggtaagatt gagttcgcga
     7141 ttaagcgcct catcttcatt accttctgtc ggcttatcat taatcgccgt cagccgcgcc
     7201 cgcactaccg gataaaatga ttccgggact atatgatgtt ccgcgaggaa cgcttttagc
     7261 ggtgctacct gttctgtggc gatgttaatt aaaaagtagt tcgggctttc tggcggtagc
     7321 tgctgctgcc agcggtcgag cagatcgcca cgcagcacca gcagcagtgc cagcagcata
     7381 aaggagagcg aaaatgccga aagctggctt aacgttgacc acggctgacg taacaggcgg
     7441 ctaaccgcca ggcgcagagg cagcgatttc agcgtcatgc ggcgaagtac attcagcagc
     7501 atccagccca gcacaccgca cagcaaagcc agtactaccg cgcccgccag caccgcccaa
     7561 agcagcatgc tgccacccat taatccggcg agcagcagca caaccaccac actgacaatc
     7621 ggcagataaa acttcagcgg ccagacgttc gcgaccacat cattacgtaa tacgcgtaaa
     7681 ggctgcgttg ccagcaacaa gcggtatggt cgtagcccca ccagcagcga gatgaccgtc
     7741 atggtgccaa gcgcccacag ccacggccag aggctggctg gcggtagtgc agcaggcaga
     7801 acgggcttga gcagcaccat caacacgttt tcgaacaaca ggcctatggc cccaccggta
     7861 acggctgaaa gcgtcagcac catcaaccac tgaccgacga ttagcttacg cagttgcgct
     7921 cgccctgccc ccagcgtttt gaggatcgcc accagatcgt agcgactgcg acagtaatga
     7981 ttcatcgcta ccgccaccgc tgccactgcc agcagcaagg tcagaagcgc cgaaagcagc
     8041 aggaactgtt gcgagcgttc catcgatcgc cccagcgcgc cttcgtcctg ttccagaccg
     8101 taccagcgtt gttcgggttt aagctgaggt aacaaccatt tctcatagcc gtcgagctgg
     8161 ttctcgttgc cgccgaattt ataacgccag gtgacccgac tccccggctg cacggctccg
     8221 gttttatcga catccgccag attcatcatc agacgcggag ccatctggaa ggggttaaaa
     8281 ccggaatccg gttcctgaat cacttctccg gcaatccgca aggtggcatc gcccacgtca
     8341 atggtgtcgc ccgttttcag gttaagcagt gccatcaggc gtggggccag caatacgctg
     8401 cccgcctgcg gtttcaggcc agggggatta gtttgcagac cgccatacat cgggtagata
     8461 tcatccaccg ctttgacgtt cgccagctgc ggtgtgtcgc ctgcaaaggt cattgtggcg
     8521 aaagtcagct gcttgccgac tttcaggccg cgcttttgcg cttcctccag ccacgcttgc
     8581 ggcacttcgc gtgaactgcg caacgcccga tcgcccgcca taaactcacg gctttgctgg
     8641 cttaagccct tctccatgcg atcgctgata ttgcccagcg ccagcacgca ggccaccgcc
     8701 aggcttagcg ccagccagac aattaatagc gacggcgaac gccattcgcg ccagaaccaa
     8761 cgtgcaatca tgcttcctcc tgcaactgcc cgttcaccag ccgtaagcag cggtcgcagc
     8821 gtgccgccag ttgcaggtcg tgggtcacca taatcaacgt ggtgccatgt tcacggttga
     8881 gggaaaacag caggtcggca attttatcgc ccgtctggcg gtcaaggttg ccggtgggtt
     8941 cgtcggcaaa cagcacatca ggtcgaccat taaaggctcg tgccagcgcc actcgttgct
     9001 gttcaccgcc ggaaagctgt gccggaagat gatccagacg tttacccagc cctaactgtt
     9061 cgagcaacgc tttcgccccg ttacgacttt ccgcgctact ctcaccgcgc agcagagccg
     9121 gaagctcgac gttttccagc gcgttaaggg taggaattaa cataaatgac tgaaaaacaa
     9181 agccgacgtg cttcgcgcgc aactttgccc gcgcttcttc gtccatatta tgtagcggtt
     9241 gtcccaccag actcacttcg ccactgctgc cgtcatcaag cccggcgagg atcgccagca
     9301 aggttgactt acccgatccc gactcgccca ccagtgcgat ggtctcgcca cgtttgacaa
     9361 ccagctcaac tccggtgagg atggagagtt catgctcccc ctgaccgacg gacttcttaa
     9421 gatgatgaac ttcaacaatg ttttccgctg gcatttgccc ttcctgttcc tggtcctgtt
     9481 aaccttccgt gccgccgcag cggacacgtt attgattctg ggtgatagcc tgagcgccgg
     9541 gtatcgaatg tctgccagcg cggcctggcc tgccttgttg aatgataagt ggcagagtaa
     9601 aacgtcggta gttaatgcca gcatcagcgg cgacacctcg caacaagggc tggcgcgcct
     9661 tccggctctg ctcaaacagc atcagccgcg ttgggtgctg gttgaactgg gcgcaatgat
     9721 ggtttgcgtg gttttcagcc acagcaaacc gagcaaacgc tgcgccagat tttgcaggat
     9781 gtcaaagccg ccaacgctga accattgtta atgcaaatac gtctgcctgc aaactatggt
     9841 cgccgttata atgaagcctt tagcgccatt taccccaaac tcgccaaaga gtttgatgtt
     9901 ccgctgctgc ccttttttat ggaagaggtc tacctcaagc cacaatggat gcaggatgac
     9961 ggtattcatc ccaaccgcga cgcccagccg tttattgccg actggatggc gaagcagttg
    10021 cagcctttag taaatcatga ctcataaagc aacggagatc ctgacaggta aagttatgca
    10081 aaaatcggtc ttaattaccg gatgttccag tggaattggc ctggaaagcg cgctcgaatt
    10141 aaaacgccag ggttttcatg tgctggcagg ttgccgaaaa ccgaatgatg ttgagcgtat
    10201 gaacaacatg ggatttaccg gcgtgttgat cgatctggat tcaccagaaa gtgttaatcg
    10261 cgcagcagac gaggtgatcg ccctgaccga taattgtctg tatgggatct ttaacaatgc
    10321 cggattcggc atgtatggcc ccctttccac catcagccgt gcgcagatgg aacagcagtt
    10381 ttccgccaac tttttcggcg cacaccagct caccatgcgc ctgttacccg cgatgttacc
    10441 gcacggtgaa gggcgtattg tgatgacatc atcggtgatg ggattaatct ccacgccggg
    10501 tcgtggcgct tacgcggcca gtaaatatgc gctggaggcg tggtcagacg cgctgcgcat
    10561 ggagctacgc cacagcggaa ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg
    10621 cttcaccgac aacgtcaacc agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc
    10681 cgcccgcttt acgttgggac cggaagcggt ggtcgacaaa gtgcgccatg cttttattag
    10741 cgagaagccg aagatgcgct atccggtaac gctggtgacc tgggcagtaa tggtgctcga
    10801 gcgcctgctg cctgggcgcg tgatggacaa aatattgcag gggtgagttg aagcgcgcgc
    10861 ttaagccccc atgtcaaaga aaatatcgac aacagagagc gactccatgt ccgtagaaaa
    10921 tattgtcaac attaacgaat ctaacctgca acaggttctt gaacagtcga tgaccactcc
    10981 ggtgctgttc tatttttggt ctgaacgtag ccagcactgt ttgcagttaa ccccgatcct
    11041 ggaaagcctc gcggcgcagt acaacgggca atttattctg gcgaagctgg actgcgacgc
    11101 ggagcagatg attgccgccc agtttggtct gcgcgcgatt ccgaccgtgt atctgttcca
    11161 gaacgggcaa ccggtagatg gcttccaggg gccgcaaccg gaagaggcga tccgcgccct
    11221 gctggataaa gtgctgccgc gcgaagaaga gctgaaagcg cagcaggcga tgcaactgat
    11281 gcaggaaggc aattacaccg atgccctgcc attgctgaaa gacgcctggc agttgtcgaa
    11341 tcagaacggg gagatcggcc tgctgctggc agaaacgctg attgcgctga accgttctga
    11401 agatgcggaa gcggtgctga aaaccattcc gttgcaggat caggacaccc gctaccaggg
    11461 gctggtagcg caaatcgaac tgctgaagca ggcggctgat aagccggaaa ttcaacagtt
    11521 gcaacagcag gtggcggaga atccagaaga tgccgcactg gcgacgcaac tggcgctgca
    11581 actgcatcag gttgggcgca atgaagaggc gctggagttg ctgttcgggc atctgcgtaa
    11641 agatctcacc gccgcagacg gtcagacgcg taaaacgttc caggagatcc tcgctgcgct
    11701 gggtacgggt gatgcactgg cgtcgaagta tcgccgccag ctgtatgcat tgttgtattg
    11761 atattccgtt gttcggcaca tgaaccacaa cgccggatgc gatgctgcgc atccggcatc
    11821 gcatcatttc ttcttcaatt gagtcaccac caactggtgg cgcgaattat aaaacttacg
    11881 ataggttaaa tagcaggcaa taatggtcga caagctggcg gttgagagca gcataaaggt
    11941 caccataatc tgatatttaa tcgccttcac cggatcaatc ccggcaaata tcagcccgga
    12001 catcattcct ggtaaactca ctaagccaac cgtttttgct gaatcgaccg tcggaattaa
    12061 agccgcgcga atactgtcgc gaatcaatat cgctgaagcc tgcttcggcg tcgcgccaag
    12121 actcagtttc tcctgaatct gttgctgttc gctaatgacc cgttgcccta aattgttgta
    12181 acacaacccc accgctacca tggcgttacc ggcaatcatc ccggcgacag ggatcacctg
    12241 catcgggata aattcaatcg cccctgagag aatcagcacc gccagggtaa ttcccgcccc
    12301 gaccgtaatg gcaataaacg atgagataaa agctttagca atatatttac tgcgtttttg
    12361 cgcgttccac gccgcattaa agcagataaa taacaccatc aataatgtca ggctggcatc
    12421 atccacgctg aaaatatact tcagcacata gccgacaata atcagctgaa ttatcgctcg
    12481 cccgacgctc catagaatat ctttctccag cgccagtttt tctttatggc taattaagat
    12541 tgccaccacc accagcatta atgccagtgc taatgattcg ttagtaatat tatgcgagtt
    12601 catagcgtgc ttcctgcatt tctccggcat gcggttgcag tgtaatcact ttatccgcat
    12661 gattaatttc gtctttatcg tgtgtcaccc acagcacggc aatattttgc tcgcgcacat
    12721 aacgatggat catctcattg acgttatgtt tattactttc atccagcgca ctggttattt
    12781 catccagcaa taaaaccttc ggcataaatt gcaggttacg aatcaatgag atgcgttgtt
    12841 tttcaccacc agatagctcg gcgatattct tcgtcaaaat gctgtccggc aaagcgaagc
    12901 gttcgagaaa atcgagaaaa atggccgggt caggctgctg gtcacggatc tgccagggaa
    12961 agatcagatt atcgtatacc gtatcgccaa acagcgtcgg cgtctgggcg cagtaagaga
    13021 cttgctggcg gtagatttct ggttttagtg tgctgacatc ctcaccttca aacagtaacg
    13081 ttccgctggt tgggctgatc aatgaagcaa ctatttttag cagcgtactt ttgccacaac
    13141 cagaaggacc ggtaattaac ttaaattcgc cagcacgcag cgaaaaattg atgttattaa
    13201 gaatcttcgt atcacccgcc agatatccta cgttttgtag ctgaagcaaa ggactatttt
    13261 cctgcatcgc tgttcccttt ttctgatttt tactaaaaac agtttatcct tcgcaggaat
    13321 aagggggaac tctctttcag taatcaggta taatttgttt aatcaagcag caattttgct
    13381 gattatggac ggtacaacag gaggtttttc cgatgcttat ctttatcccg attctcattt
    13441 ttgtcgcgct ggtcattgtc ggcgcgggcg tcaaaatcgt gccgcagggc tatcaatgga
    13501 cggtagaacg ttttggtcgc tataccaaaa cgttacagcc ggggctcagt ctggtggtgc
    13561 cgtttatgga tcgcattggt cgcaagatca atatgatgga gcaagtgctc gatatccctt
    13621 cccaggaagt tatctcgaaa gataacgcca acgttaccat cgacgcagtc tgttttattc
    13681 aggtgattga cgcgccacgc gctgcctatg aagtcagcaa tctggagctg gcgatcatca
    13741 acctgaccat gactaacatc cgtaccgtgt tgggttcaat ggaacttgac gaaatgctct
    13801 ctcagcgcga cagcatcaac tcacgcctgc tgcgtattgt cgatgaagcc accaacccgt
    13861 gggggattaa agtcacccgt attgaaattc gcgacgtgcg cccaccggca gagcttatct
    13921 cttcaatgaa cgcgcagatg aaagcggaac gtaccaaacg cgcttacatt cttgaagcgg
    13981 aagggatccg tcaggcggaa atcctcaaag ccgaaggtga aaaacagtcg caaatcctga
    14041 aagcggaagg cgaacgtcag tcggcgtttt tacaggctga agcgcgtgaa cgttccgccg
    14101 aagcagaagc ccgcgccacc aaaatggtgt ctgaagctat cgcctccggt gatattcagg
    14161 cggtgaacta cttcgtagcg caaaaataca ccgaagcgtt acagcagatc ggttcctcca
    14221 gtaacagcaa agtagtgatg atgccattag aggccagcag cctgatgggg tcgattgccg
    14281 ggattgccga gctggtgaaa gacagcgcca acaaacggac tcagccatga tggagttaat
    14341 ggtcgttcat ccacatattt tctggctcag tctcggcggg ttgctgctgg cagccgagat
    14401 gctgggcgga aatggttatt tgttgtggag cggcgtggca gcggtgatta ccggcctggt
    14461 ggtctggctg gtgccgctgg gttgggagtg gcaaggggta atgtttgcca tcctgacgct
    14521 gctcgccgcc tggctgtggt ggaaatggtt gtcgcggcgg gtgcgcgaac aaaagcacag
    14581 cgatagtcat ttaaaccagc gcgggcagca gctgattggc cgacgttttg tgctggaatc
    14641 tccgctggtc aacgggcgcg gtcatatgcg cgtcggtgac agttcatggc ctgtcagcgc
    14701 cagcgaggat ctcggtgcag gtacgcatgt tgaagtcatt gcgatagaag ggataaccct
    14761 acatatccga gccgtctcgt cttaatcacc ctgcccgatg atgacagcag ccggagagat
    14821 tttcgataat cgggcagtcg gcgctgtcat cgccagggca ggcattcgcc agtgccagca
    14881 gctggtcgcg catggattgc agctcctcaa tgtgtcgttc gatctccgcc accttctcca
    14941 gcgtgcgccg tttgacgtcg gcgctgtgcc gctgcgggtc gttaaacaga ttcaccagct
    15001 cgccgctctc ttccaggtta aagcccacct gccgtgcctg gcgcagtaag gtcagttcgt
    15061 tgagatgctg ctgcgtgtag gtgcgataac cgttttcgct gcgcatcggc ggcgtcacca
    15121 gccccttctc ttcatagaag cgaatggctt tgctggtcag gccggtaatt tttgctacat
    15181 cgctgatgtt catcgttcgc gcaacgcctc ctgtgctttg ttaaagggtt tcaacagata
    15241 ttcaagcacg tttttttgcc agtgcgaata tcgaccgcca ccacaataat acaggaagta
    15301 ctgcgcacag agaggtgtta tacggtttta ttacgcttca tgaccattgc cacaataagg
    15361 ctgagtaaga ttgcaccaat aatccatatc aacgcacggc tgccctggga ccacaggctg
    15421 tagataaagc caacgatcac caacaacatc accaacgtac caacgataag aatcggcaac
    15481 gaggcgtgaa tatcatgacg caggcggatc gccaccacaa acaccgccag atagcagatc
    15541 aaaaaggtgg cgctggcaac gctggcgagt gagtctaaat ttagcgccgc cgtcatcagc
    15601 attatcaaca ccacgacgat gatattgccc caggtactct gccgccacag ggatttattc
    15661 attagcttcg gcagttcgcg ttcgctgccc atgttgtcca tgatgttaaa cacggcgaac
    15721 aggttcgcgt taatgcctga agccgtcgcc agtaaagcgc cgatgacgac gatcacataa
    15781 cccacatgcc cgagcagcgg agaagcagcc tgcgctacgg cggtatcagc atatttttct
    15841 aactctaatg ccgatacatc gctaagcaaa accagcgcca gcgagatata aagcaacgtg
    15901 gtaacgccaa tcgccaccag aaacgcccgt ggcataatga cctgcggatc tttcacttta
    15961 tccgccgcgt tcgccatcat gccaaagccc gcataggcaa ggaaggttat cccaatacag
    16021 gagaagaacg caccggagct ggggggcgca gagacggaaa tatgcgccgg ttgcagcgac
    16081 cagacaccgg caataatcag caataacagg atcatcattt taatgccgac gaggatcact
    16141 tccagccgcc ctaccgcatg gttgcttaag gagttgaaaa gcgtcatcac cgcaatgatc
    16201 cccaacgcgt agagcaaaat aaggtgctcc tcctggctgc cttcatgcaa aaactgcacg
    16261 gcataagcgc caaaagcacg ggcaaccatg gcgatgctca ccgccagcgt caacaggtat
    16321 agcaacgaga gcgctagcga aaagacgccg ttgcctaatc cgcgacgaaa aaagtcgata
    16381 ataccgccat tgctgggata gctcgccccc agacgcgcat aggcataacc ggaaaacata
    16441 gccacaatac cgccaaaagc aaaggcgccc caggtcgagg cttccattag caacgcagcc
    16501 tgccccagca gcgcgaagat ccccgccccc accattgccc caataccgat ggaaacgacg
    16561 ttccatagac cgaggggttt gttaccgtta ttaccttccg tgttcatcat gatcagccct
    16621 taaacacgtt atagccgagt tgcttagcga ccgatgccac cattttttga ccgcgaacac
    16681 tattgccttc ttcgtccagc ggtggtgaga acgcggcaat tcccatcacg ccagggacga
    16741 ccgccagaat accgccacct acaccgcttt tgcccggtaa accaacgcga tacgcccagt
    16801 caccggagcg accatacagc ccttccatca tcatttcggc cagaatgtac ggcacgttgt
    16861 cggcctgaag aacgcgttta tgcgtcaacg gattcacacc acccgccgcc agcgtcgcgc
    16921 caagcgttgc cagttcaaca gtattgatga gcgtagagca ctgacgggtg tacacgtcac
    16981 aggcttccat tgcatcacaa tagagatatc cggcggagta cagcagccag gcaatggccc
    17041 gattatggaa gttggttgtt tgttccgact ggttgacttc gtcagagagc gctacctgct
    17101 caccagccag ttgctgttgg atatgtaaaa ttcgctgcca gcgttgttcg gcgttttcag
    17161 cgttaatcag gctggtggtg gcaatagcgc cagcatttac cagtggcgaa agcggtttgc
    17221 cgccatgcaa ctctaaggcg ataactgagt taaagggcaa tccggtcggg tcagcgccaa
    17281 ttttgtcctg taccgcctgc gggccgacat cttctaacgc aagggctaac gtacagactt
    17341 tagagatgga ttccagtgca aagcggtaat cactgtcacc cgcactatag acgttgccat
    17401 cgcaggtcac gatagccact gccgccagtt gacctggtac attcgccaga aagggaatgt
    17461 aatcggcatt ttgtccgccg ttaagtgagt gaaattgggt gtaagcctga tccactgcct
    17521 gctgtaattt gtttgcatct aacatctttt gttaactcct ttttatagat gcgggaggta
    17581 attcctcacc ccagtgccga ttttcaggca tcctgattta acttagcacc cgcaacttaa
    17641 ctacaggaaa acaaagaaat aaatgtctaa tcctgatgca aatcgagccg attttttaat
    17701 ctttacggac ttttacccgc ctggtttatt aatttcttga ccttcccctt gctggaaggt
    17761 ttaaccttta tcacagccag tcaaaactgt cttaaaggag tgttttatgt cacaaactat
    17821 cgacctgaac ctggacggcc tgtcctgcgg tcactgcgtt aaacgcgtga aagaaagtct
    17881 tgaacagcgc ccggatgtgg aacaagcgga tgtgtctatc actgaagcgc acgttaccgg
    17941 gactgccagt gcagaacagc tgatcgaaac catcaaacaa gcgggttatg acgcatctgt
    18001 aagccaccca aaggctaaac cgctggcgga gtcatcaatc ccgtcggaag cactgacagc
    18061 ggtttctgag gcgcttccgg cagcgaccgc cgatgacgat gacagccagc agttgctgct
    18121 gagcggcatg agctgcgcca gctgtgtcac ccgcgtacaa aatgcgctgc aaagcgtacc
    18181 gggcgtcact caggcacggg taaacctggc ggagcgtact gcgctggtga tgggcagtgc
    18241 ctccccacaa gatttagtgc aggcggtgga aaaagcgggc tacggcgcgg aagcgattga
    18301 agatgacgcg aaacgccgcg aacgccagca agaaaccgcc gtcgctacga tgaagcgctt
    18361 ccgctggcag gcaattgtcg cactggcggt gggtatcccg gtgatggtct gggggatgat
    18421 cggcgataac atgatagtca ccgccgacaa ccgcagcctg tggttggtta tcggcctgat
    18481 aaccctggcg gtgatggttt tcgccagcgg ccatttttac cgcagtgcat ggaaaagcct
    18541 gctgaacggt gcagcgacga tggatacgct ggtggcgctg ggaacgggcg tggcgtggct
    18601 ctattcgatg agcgtcaacc tgtggccgca gtggttcccg atggaagcgc gacatcttta
    18661 ctatgaagcc agtgcgatga ttatcggtct gatcaatctc ggccatatgc tggaagcacg
    18721 cgcacgtcag cgttcttcta aggcactgga gaaattactc gatttaaccc caccgacggc
    18781 gcgcctggtt actgacgaag gtgaaaaaag cgtgcctctg gcagaagtgc agccaggtat
    18841 gttgctgcgc ctgacgaccg gcgatcgcgt gccggtagat ggcgagatta cccagggcga
    18901 agcatggctg gatgaagcga tgctgacggg cgaaccaatc ccgcagcaaa aaggcgaagg
    18961 cgatagcgtc catgccggga cagtggtaca ggacggcagt gtgctgtttc gtgccagtgc
    19021 ggttggcagc cataccacgc tatcaaggat cattcgcatg gtgcgccagg cccagagcag
    19081 caagccagaa atcggtcagc tggcggataa aatctcagcc atatttgtgc cggtagtagt
    19141 ggttattgcg cttgtcagtg cggcaatctg gtatttcttt ggtccggcac cgcagattgt
    19201 ctataccctg gtgattgcca ctacggtgct gattatcgcc tgcccgtgtg cgctgggact
    19261 ggcgacgccg atgtcgatta tttccggcgt cgggcgggcg gctgagtttg gcgtgctggt
    19321 gcgggacgcc gacgcgctgc aacgcgccag tacactcgat accgtcgtgt tcgataaaac
    19381 cgggacgctg accgaaggga agccgcaggt tgtcgcagtg aaaacatttg ctgatgttga
    19441 cgaagcgcag gcgctgcgtc tggcggcggc gctggagcaa ggttccagcc atccattggc
    19501 acgagcgatc cttgataaag caggcgatat gcagctaccg caggtcaacg gtttccgcac
    19561 attgcgcggg ctgggcgtga gcggtgaagc agaaggtcat gcgttattgc tgggtaatca
    19621 ggcgttgtta aatgatcagc aagtcgatac taaagcgatt gaagccgata tttcagctca
    19681 ggcatcgcaa ggagcaacgc ctgtgctgct ggcgattgac gggaaagcag tagccctgct
    19741 ggctgtacgc gatccgttgc gtagtgatag cgtggcggcg ctgcaacgcc tgcataaagc
    19801 gggatatcgt ctggtgatgt tgaccgggga taacccaacc accgccaatg cgatcgccaa
    19861 agaagctgga attgatgaag tgattgccgg agtgctgccg gacggtaaag ccgaagcgat
    19921 caaacgtctg caaagtgaag gacgtcaggt ggcaatggtg ggcgacggca ttaacgacgc
    19981 gccagcgctg gcccaggcgg atgtcggcat tgcgatgggt ggcggcagtg atgttgccat
    20041 tgaaaccgcg gcgattaccc tgatgcgcca tagcctgatg ggcgttgcgg atgcgctcgc
    20101 gatttcccgc gcaacgctgc gcaacatgaa gcagaacctg ctcggtgcct ttatctacaa
    20161 cagcatcggt attccggtcg ccgccggtat tttgtggccg tttactggaa cactgcttaa
    20221 cccggtagtt gccggagcgg caatggcgct ctcgtcaatt accgtagtaa gtaacgccaa
    20281 ccggttgctg cggtttaaac cgaaggaata agtacgtcat tgcggatgcg ctgctttagg
    20341 gcggcgcatt tggcatcgac gcatgactgc cggatgcggc gtaaacgcct tatccggcct
    20401 acggttcagg cacaggtttg taggcctgat aagacgcgta agcgtcgcat caggcaatgg
    20461 tgctcggatg ccggatgcgg cgtgaacgcc ttatccggcc tacgagatga agcgatgttt
    20521 tggccttacg cgaccttttt tgcacgctct tcacggcgtg ccaaatattc agccactgtt
    20581 tcaatccgtc ccaattcttc ctgggtgcgc atgttgtttt caacttccca aagatcaact
    20641 gccgcctgga ggtttagcct aaaatcgact gtggtatcaa aaactttcgc cagacggaat
    20701 gccatctcag tggtgagttt acgattgtta ttgatcagtg cactgacgcg attacgatga
    20761 acatgcagca actctgctaa ctcattgatt ttcaaatcga gcggttccag atattcatag
    20821 agaagaatat ctccgggtgt cgtcggtttt cttgttgcct gtttcatggt ttttaactgt
    20881 tgcttccctg tgaatagcga tgcaagaaca tactgaatca tacaaacaca ttaaacaagt
    20941 ccccaactcc atttgcgcca tccctcaccg cgcgctagca tgatatttca caaagggagg
    21001 tcgtatggat ctgttgtacc gggtaaaaac gctttgggcc gcgctgcgcg gtaatcatta
    21061 cacctggcct gccatcgata tcaccctccc cggcaatcgc cattttcatc tgattggcag
    21121 tattcatatg ggtagccacg atatggctcc cctgcccacc cgtttgctca aaaagctcaa
    21181 aaacgccgat gcgctgatcg tcgaggcgga tgtttccacc agcgatacgc cttttgctaa
    21241 tttgcctgcc tgcgaggcgc tggaagagcg cattagcgag gagcaactcc aaaacctgca
    21301 gcacattagc caggagatgg gcatttctcc ctcactcttt tctacccaac cgctgtggca
    21361 aatcgcgatg gttcttcagg cgacgcaggc acaaaaactg gggctgcggg cagaatacgg
    21421 tatcgattac cagctattgc aggcggcgaa gcaacaacat aaacccgtga ttgaactgga
    21481 aggggctgaa aaccagattg ccatgttgct ccagctccct gacaaaggac tggcgctgct
    21541 ggacgatacg ctgacccact ggcataccaa cgcacggttg ctgcaacaaa tgatgagctg
    21601 gtggctgaat gcaccgccgc aaaataatga tataacgctg cccaatacgt tcagtcagtc
    21661 gctgtacgat gtgctgatgc atcagcgaaa tctcgcctgg cgggataaat tacgcgccat
    21721 gccgccgggg cgatatgtgg tcgcggtcgg tgcactacac ctgtatggag aagggaattt
    21781 gccgcaaatg ttgcgctaaa aaaatggcca atatcgctat tggcccgtca aagaggaatt
    21841 tcattttttt attattatgc cgtcacttta agcgacggtg taactcgatt gtcgctcaat
    21901 ctcaccatcc tgccaatact cttaggcaag atgactcttt tttttggacc gccatcaggc
    21961 gtatgcttgc ttcgtttttt tgtttaaggc aggaaaatta ccatgacacc cgcagttaaa
    22021 ttactcgaaa aaaacaagat ttcgtttcaa atccatacct acgagcacga tccggctgaa
    22081 accaattttg gcgatgaagt cgtcaaaaaa ttaggtttga atccggatca ggtctacaaa
    22141 acgctgctgg tggcagtgaa cggtgatatg aaacaccttg ccgtggccgt tacgccggtc
    22201 gcaggtcaac tggatcttaa aaaagtagca aaagcgctgg gtgccaagaa agttgagatg
    22261 gccgatccga tggtcgcgca gcgttcgacg ggatacctgg ttggggggat tagcccactg
    22321 gggcaaaaaa aacgtctgcc aacgattatc gacgcccccg cacaagaatt tgccactatt
    22381 tatgtttccg gcggcaagcg cggactggat atcgaactgg cggcaggcga tctggcaaag
    22441 atcctcgatg ccaaatttgc tgatatcgcc cgccgcgatt aagcattgtg ccggatgcaa
    22501 acatccggca ctttcggatt actgccagct cacctcacct ttcggttcat aaacactcac
    22561 atccagcggc gagcttttct ggatatacgc tttcagcact tcggcatcaa taaagccggt
    22621 attcacatag cccggtttgt tatcaaggcg cggatatcca tcaccgccgg tggcattgaa
    22681 gtttaatgtc gccatacggt aagttttcgc cggatcgacc ggttcgcctt tgattttaag
    22741 gtcgttcagt ttgccgtctt tcgccacaaa gctaacgttg gcaaattgcg ggtaggcacc
    22801 tgaatctggc ttcatctgcg cgacggcggt caggtaatca atcacctctt taccggtcat
    22861 gtcggcatac accaccacat tgccgaatgg ctgcactttc agcacgtttt tatagctgat
    22921 atcgcctgct tcgatagaat cacgaattcc gcctccgctc atcaccgcaa agtcggcacc
    22981 agtgcgatcc atttgggctg ccagaatcaa ccgccccata ttggtctgta caaaacgcac
    23041 tttgtcacga tcgccttcca gacgaccatt ggttttgcct attttcactt ccagctgcgc
    23101 tttgcctttg ttctggaacg gtgataacag cgagatcatt tgctggtttt cagcgatttc
    23161 aggagtgtaa agcacgcgct cgcttttccc gtcttcccag gtcactttct tcttcaggtt
    23221 caccggaatc agctggtagt taaccatttt catttcgcca ttacgaaact caaaatcagc
    23281 ccgtcccacg tatttgcccc actcatgcgc ctgcacaatc cagatgccgt tttgttgatc
    23341 tggtttgcat ggcgtacccg gcacgtaatc gacctgtttt ttgttttctg ccgccatgca
    23401 gaccggatct tgcgagtgac caccgacgat catcgccagc gatccggcag gcagcgcgcg
    23461 tgccatctcc acatcgcccg gtgcgttaga gccgtgctca ccattatcgt aatgccccat
    23521 atgggtcgcc gcgataataa tgtctggctt ttctgtctgt tgcagctcct gaatcaccag
    23581 cttcgcttca tcggcgggct tacgaaattc gatatcagtg aagtattcca ggttaccaat
    23641 ttttgctgtg tcatcggttg tcagcccaat aacggcaatt ttcagatcct gacgcttaaa
    23701 cagcgcccac ggtttaaaca ggcgctcgcc agtacttttc tggtagatat tcgcggaaag
    23761 caacgggaac ttggcccact tttcctgctg gcgtaatacg gtgagcggat tatcaaattc
    23821 atgattaccg atcgccatcg cgtcatagcc caccagattc ataccgcgaa aatcaggttc
    23881 ggcatcctgt aagtcagact cgggcacgcc agtgttaatg tcgccaccgg aaagtagcag
    23941 cacgctaccg ccttcagccg caacctcttt gcggatacca tccaccagcg ttttttgcgc
    24001 cgccagacca tattcgccat attcattgcg ccaaaaatgc ccatgatgat cattggtatg
    24061 cagaactgta attttgtagg ttttatcctg ctcatacgcc agagcagttt cactcgccag
    24121 tgtaaatgtg gttaacagcg ctaacgccac gccccgctgc aataatttca tacttctctc
    24181 cctgacctga tttcaacgtg ttgaaaaata gtatgacgca acatcataga caagtctgcg
    24241 tccggtgaag ataaatctgc atccagatta gaaattctgt attcggcgtt gagacttcct
    24301 tcagacgacg catacaggta tgttagtcgg gtaataatca caaataagat cattctttac
    24361 atcgcaaccc tgaaaaagtg actttcctat ggcaatgagt gaacaaaccc agcctgtggc
    24421 gggcgcggct gcgtcaacga ccaaggcccg aacatcgttt ggtattttag gtgctatcag
    24481 cctctcacat ctgctgaacg acatgatcca atcgctgatt ctggcgattt atccgctgct
    24541 tcagtcagaa ttttctctga catttatgca gattggcatg ataaccctca ccttccagct
    24601 cgcctcttcg ctactgcaac cagtggtcgg ctactggacc gataaatatc cgatgccgtg
    24661 gtcgttgcca attggcatgt gctttacctt aagtggtctg gtgctgcttg cgctggcggg
    24721 cagttttggc gcagttctgc tggcagcggc gctggtcggt accggttcat cggtctttca
    24781 tccggaatct tctcgcgtag cccgtatggc ttccggcggg cggcatggcc tggcgcaatc
    24841 tatctttcag gtcggcggca actttggcag ttccctggga cccttgctgg cggcggtgat
    24901 tatcgcgcct tatggcaaag gcaacgttgc ctggtttgtg cttgcggcac tgctggcgat
    24961 cgtggtgttg gcgcaaatta gccgttggta ctcggcacag caccgaatga ataaaggaaa
    25021 acccaaagcg acgataatca atccactgcc gcgcaacaaa gtggtactgg cagtcagcat
    25081 tctgttaatc ctcattttct cgaaatattt ctatatggcg agcatcagca gctattacac
    25141 cttttatctg atgcaaaaat tcggattatc tatccagaat gcccagcttc atctgtttgc
    25201 cttcctgttt gccgttgcgg caggtacggt gatcggcggg cctgtagggg ataaaattgg
    25261 acggaaatat gtgatttggg gctctatcct cggcgttgcg ccgtttacgc tgattttacc
    25321 ctacgcccgc ctgcactgga cgggggtttt aacggtgatt attggattta tcctcgcttc
    25381 ggcattctct gccattctgg tctacgctca ggagctactt ccgggacgta tcggtatggt
    25441 ttctggactc tttttcggtt ttgctttcgg catgggaggt ctgggagcgg cagttctggg
    25501 gcttatcgcc gatcacacca gcatcgagtt agtctataaa atctgtgctt tcctgccact
    25561 attggggatg ttgaccatat tcctgcctga taaccggcat aaagactgat ttcattgcag
    25621 ccaaaggcaa actttggctg catcgtttta cagtcgccat aagcctttcc tctgttaaac
    25681 cgccttctgt tttccgaacc gtgctttttt tgcagctaat tccatttttc tgcaaaattc
    25741 aacaattatt cataaacatc atcaatgaaa ttgtcataaa ctattacctg taattttggt
    25801 tttcccagcc aaaaatggac aataaccacc aggaaaagga gacggaatgc atcacgccac
    25861 cccgcttatc accaccattg ttggcggcct tgtgctcgcc tttatcctcg gcatgctggc
    25921 caataaacta cgtatttctc ctctggtggg atatctgtta gcgggtgtgc tggcaggacc
    25981 attcactccg ggctttgttg ccgataccaa gcttgcgccg gaactggctg aactgggcgt
    26041 tattttgctg atgttcggcg tcggtttgca tttttcgctc aaggatttga tggcggtaaa
    26101 gtccatcgcc attcccggcg cgatcgccca gatagccgtg gcgacgctgc tgggtatggc
    26161 gctctccgcc gtgctgggct ggtcgttaat gaccggtatc gtgttcggct tatgtctttc
    26221 caccgccagt accgtggtgt tactgcgcgc acttgaagaa cggcaattaa tagacagtca
    26281 gcgtgggcaa atcgccatcg gttggttgat tgtggaagac ctggtgatgg ttctgacgct
    26341 ggtgttgctg cccgcagtgg caggaatgat ggaacagggc gatgtgggct ttgccactct
    26401 tgcagtcgat atggggatca ccatcggcaa ggtgatcgca tttatcgcca ttatgatgct
    26461 ggtaggtcgc cgtctggtgc cgtggattat ggcacgcagc gcggcaaccg gttctcgcga
    26521 gctgtttacc ctgtcagtgc tggcgctggc gttagggatt gcctttggtg cggtagagct
    26581 gtttgatgtc tcctttgcac tcggtgcgtt ctttgccggg atggtactga acgagtctga
    26641 attgagtcac cgtgccgccc acgatacgtt gccattgcgc gacgcgtttg cggtgctgtt
    26701 ttttgtctca gtcgggatgt tgtttgatcc gttaattctg attcagcaac cgctggcagt
    26761 gctggcgacg ctggcaatta ttctgtttgg taaatcggtc gcggccctgt tccttgtccg
    26821 tctgtttggt cactcccaac gtacggcatt aaccatcgcc gccagcctgg cgcagattgg
    26881 tgagttcgcg tttatcctgg cgggactggg aatggcattg aatttactgc cgcaggccgg
    26941 acaaaacctg gtactggcag gggcgatcct gtcgattatg ctcaacccgg tactgttcgc
    27001 actactggag aaatatctgg cgaagaccga aacgctggaa gagcagacgc tggaagaggc
    27061 aatcgaagaa gagaaacaga tcccagtgga tatttgcaac catgcgctat tggtgggtta
    27121 cggtcgtgta ggcagcctgc tgggggagaa attgctcgcc tctgatattc cgctggtggt
    27181 gattgagacg tcacgaaccc gtgttgatga gctgcgagag cgtggggtcc gcgctgtatt
    27241 gggcaatgca gcgaacgaag aaattatgca actggcacat ctggaatgtg caaaatggct
    27301 gatcctgacg atccccaacg gttatgaagc gggtgagatt gtggcgtctg cccgcgcgaa
    27361 aaacccggat attgagatta tcgcccgcgc ccattatgac gatgaagtga cgtatatcac
    27421 cgaacgtggc gcgaatcagg tagtgatggg tgaacgtgaa attgcccgta ctatgctgga
    27481 actgctggaa acgccaccgg cgggtgaagt ggtgacgggg taaacggtgg ttgccggatg
    27541 taaaatatga aaaatatgta ggcatgataa gacgcgccag cgtcgcatca ggcatcgaga
    27601 gccaaatgcc agatgcggcg tgaacgcctt atctggccta cgaaccgacg ataacttaac
    27661 gatcccagta agactcttcc tggctgtctt cacgctccgg caagccgcgc gttaaacgag
    27721 gtgaatgctg gttcagtacc tgatagctca cacggttagc atatttacac acctgcgcta
    27781 acgatgaata agttaaccag gtgaatttat gtttgctgga gtttggtacg ttgctacgat
    27841 ggtagctgtt ggcggtaatg tcatgcagca acgctgccaa tgcgccatcc cccgctccat
    27901 tagtgttcat gattttttcc ggcccgccca tgtacggcgc aatgtgcgaa tagacacgca
    27961 gcggattctg gcaatccttg tggcgcatgg cgcggctaaa ctcatactgg ttgaattccg
    28021 ctatagcgcc cggcagcagc ggatgctggg ttttacgttt cgcttcgtct tcggtaaagc
    28081 ccgccatata caagccgatt ggcccggcgg tgcacagcac cagctctacc cagtccagcg
    28141 ccttgtcaga tgccaacaac ggatcgcttt ctccggtcaa cgcttcggct tcatcttcgt
    28201 tcatcgcaag gatagaaacg tggtctttga ggaattgctg ccaccactgc ggattctcgg
    28261 caatgacaaa cttggtgccc agcgtcagca ccaccggtac gttatatttc ttcgcgtact
    28321 caatggcttt catggttgct tccggcatgg gttcacccgg cttgcaacgc accagatatg
    28381 aggtgagaac cagtgccgag gctccggcaa tcacatcttc cggaatgctt tcagcccgca
    28441 gctggttcat gtggcctgga ctgatagcaa aggtacgttc cccggactcg ccaatcagcg
    28501 taaagcaacg accaatcgga ccatccacgc cttgtagata gttaagatcg gtacggctgg
    28561 aagtgttaca caggtaacga taggcataac tgccaatttc aatattgctg cacatgacgc
    28621 ccagcagcac cgaacggtcg tccgcgagca ccgagtagtt gtgcatggtg ttaccaatgg
    28681 tgccacccgc aaactgatgg gtaatcaggt ttttctgttt tagctcctga taaagcactt
    28741 cggctacgtc atcctcaatc accagtgaat gcccggcgct taatccataa cgctcaatga
    28801 attcatcatc cactttcgct tcaatatcga ccagcgtctg gtcaatacct acgacccagg
    28861 cggcgctggt ttcgttttct ggctggaatt gctgaagcag cggatcgcgt gcatttacag
    28921 ggaagtaatg tttggattta cgtttaccgg gaaatttcat ggttgttttt acgggtggtt
    28981 aactgagcgg cgaatgttaa cacaatctga cgggagcgcg cgagacgcag ccagtttcaa
    29041 tatcagcagg cgggatcgca ttttcatggc agtggttcct tacaatgacg taatttgaaa
    29101 ggagtttttg tatgaagccg gaaaacaaac tacctgttct ggaccttatt tctgctgaaa
    29161 tgaagaccgt tgtgaatact cttcagccgg atttaccgtc ctggcccgca acgggaacga
    29221 ttgctgagca acgacagtat tacacgcttg agcgccgatt ctggaatgcg ggcgctccag
    29281 aaatggcaac cagagcttac atggttccaa caaaatatgg gcaggtggaa acacgtctct
    29341 tttgtccgca gccagatagc ccggcgacgc tattttattt gcatggaggc ggttttattc
    29401 tcggcaatct cgatacccac gatcgcatca tgcgcctgct ggcaagctac agccaatgta
    29461 cggtgattgg tattaattac accctttcac ctgaagcgcg ttttccacaa gcgatagagg
    29521 aaattgtggc tgcttgttgt tatttccacc agcaggcgga ggattatcaa atcaatatgt
    29581 cccgcattgg ctttgccggt gattccgcag gtgccatgct ggcgctcgcc agtgcgttgt
    29641 ggttgcgtga taaacagatc gattgcggta aaattgcggg tgttttgctg tggtatgggc
    29701 tttacggatt acgggattcc gtgactcgtc gtctgttggg cggtgtctgg gatggcttaa
    29761 cgcaacagga tttgcagatg tatgaagagg cgtatttaag caacgacgcg gaccgcgagt
    29821 caccgtatta ctgtctgttt aataatgatc tcactcgcga agttccgccc tgttttattg
    29881 ccggggcgga gttcgatccg ctgctggatg acagccgtct gctttactag acgttagcgg
    29941 cgcatcagca gccctgtgag ttcaaactct acccaggtac gctgcatgcc tttttgcatt
    30001 attcacggat gatgaaaacc gccgacgagg ctcttcgcga cggcgctcag ttctttaccg
    30061 ctcagcttta gcgatacgcg gcaacaagat tagccatcat ttcgatatgt tccggcgtgg
    30121 cattaagcgc tggaatatat tcatattttt tcccgcccgc accgaggaag acctcacggt
    30181 tttgctcggc aatctcttcc agcgtctcca gacaatccgc agcaaagccc gggcacatca
    30241 cctgtatatg acctacgcct ttttctccga gcattttcag cgtttcgtcg gtataaggca
    30301 tcagccaggg ttcccggcca aagcgcgact gaaaggtcat catcactttt tccggtacca
    30361 tccccagtgc ggaagccagt tcgcgagtcg ttgtgcggca acgttgcggg taatcatcgc
    30421 cttcatctgc ataacgctgg ggaatgccat gataagagag cagcagcaga tccggttcgc
    30481 catgtttggc aaaagaagcg cgtacgctgt tcgccagtgc attaatgtaa tcgtggttat
    30541 cggcgtaatc acgaataaac gatatccccg gaatgctacg tttgcgcgcc agaatgcgtg
    30601 ccagttcatc ccataccgca ccgaccgtag aacaggagaa ttgcggataa agcggcagca
    30661 ccacaatatg atctacatgc tctgccagga gttcatcgac ggagctttcc agtgatggcg
    30721 agccgtagct catgcccagc gccactggcg tatccggtaa acgttgtgcc aacgcctgct
    30781 gttgctggcg gctgtaaacc atcagcggcg agccgtcttc catccagaca gaggcgtaca
    30841 gcttcgccac acgcggcgag cgcagcggca aaatcacgcc gcgcagcaac ggccaccata
    30901 acaaccgtga ggtatcaacc acgcgtctgt cgcttaaaaa ttgtttcaga tagcgtttta
    30961 ccgcttcagg tgtgggggca tcgggcgtac ccaggtttgc cagcaggata ccggttttag
    31021 tctgacgcat taccgcctct tatcgattca acttgttgat aattgtagcg gaaaagtgag
    31081 gaagaagaac taattgctgt gagaggtatt gcctgaggtg gataagcaga cctgcccgaa
    31141 agcagatctg ctgcggcttg attagccgag gattttttcc agatcagcgc gaacttcagc
    31201 aacctgcttg gtaccgtcaa ctttcgcgta tttggtgtta cccgcttccg cttctttgga
    31261 gtagtagccg atcagcggtg cagtcatctg atggtattca accagacgtt tacgcacggt
    31321 ttcttcctga tcgtctttac gggtagtcag ttcttcaccg gtaacgtcgt ctttgccttc
    31381 tactttcggc ggattgaatt taacgtgata aacacgacca gacggcgcat gaacgcggcg
    31441 gcctacgata cgatcaacaa tcagttcgtc cggtacgtcg aattccagaa cgtaatcaac
    31501 attgatgccc gcttctttca tcgcgtctgc ctgcggaatg gtacgcggga agccgtccaa
    31561 caggaaacca ttacggcagt cttcctgagc aatgcgctct ttaaccagcg cgatcaccag
    31621 ttcgtcggtg accagtttgc cagcatccat aatgtctttt gcttgtttac ccagctcgga
    31681 gccagatttg accgcagcac gcagcatatc gccagtggag atttgcggaa taccatattt
    31741 ctccatgatg aactgagcct gagtcccttt ccccgcgccc ggagcgccaa gcagaatgat
    31801 acgcattgcg aaaatcccct taaaatgtgt cgattttttt gaaaaagcga taaacgatac
    31861 caccaacagg cgattgcctc aagaaaggca cctgaggctg aaacggttaa tggcgagata
    31921 atttgcggaa agtacagatg atgaaaagaa aaatgccgga tgacacaaag gtcatccggc
    31981 atgacatcag gaaaccagca gctgattcat acgacgaata aacaggttcg gatcttccag
    32041 cgtgccgcgt tctgccagca gcgcctgatc cagcagcagt tctacccact cgctgaactt
    32101 ggcttcatct tcagtatctg ccgcacgttt caccagtacg tgatccgggt tcagttcgaa
    32161 gatgtatttc acttctggca ctttctggcc cgccgcagcg aacagtttcg ccatctgagt
    32221 gctcatttcg tccgcgtcgg tcgaaacgat cgctggcgta tcggtcagac ggtgagtcag
    32281 acggacatct ttcacgcgct cgccgagcag agctttcacg cggtcgatga acggagtcag
    32341 tgctttctcc gcttctttcg cgctctcatc aacttcgtca gccagttttt ccagcgactc
    32401 gtcaacttta gacaccgact ggaacggttt accgtcgaac tcagtcagat agttcatcat
    32461 ccactcatcg atgcggtcgg aaagcagcag aacttcgatg cctttcttac gcagcagttc
    32521 caggtgcggg ctgctcttcg ctgccgcata gctgtctgca gtgatgtagt agattttctc
    32581 ctgcccttct ttcatgcggg aaacgtagtc ttccagagac acggtctgcg cagaagaatc
    32641 ggtataagta gaagcaaaac gcagcagttt ggcgatcgct tcctggttag cgaaatcttc
    32701 cgccggacct tctttcagta ccaggccaaa ctgttgccag aaggtctggt atttttccgc
    32761 gtcgtctttc gccagttttt ccagcatttg cagcacacgc ttggtcagcg cattgcgcag
    32821 gttacgcgtt accgtgctgt cctggaggat ttcacgggaa acgttcagcg gcagatcgct
    32881 ggagtcaatc agaccacgca cgaagcgcag atagttcggc atgaactgtt cagcgtcgtc
    32941 catgataaac acgcgttgaa cgtacagttt caggccgtgt ttatgatcac ggttccacat
    33001 atcccacgga gcctgggacg ggatgtacag caggctggtg tactcctgct taccttcaac
    33061 gcggttgtgg ctccaggtca gcggatcgtt aaagtcgtgg gcgatgtgtt tgtagaactc
    33121 tttatactct tcatcggtga tttccgactt gttacgagtc cacagcgcct gcgctttgtt
    33181 gatttttgcc caggagataa cggtttcgcc atctttctct tcgcgttttt cgatctctac
    33241 cggcagcgcg atatggtcgg agtatttgct gatgatggaa cgcacgcgcc agtcatcgag
    33301 gaactcgtct tcgccttcac gcagatgcag ggtgatttca gtaccacgat cttctttggt
    33361 gatatcggca acggtatatt caccttcgcc agccgattcc cagaagacgc cgttttctgg
    33421 tttttcgcct gccgcgcgag tacgcacggt cactttgtcg gccacgataa acgcagagta
    33481 gaaaccaaca ccaaactgac cgatcagctg gctgtctttc gcttggtcag aacccaggga
    33541 ttcgaggaat gatttggtac cggatttagc gatagtcccc agatggtcaa tcacttcgtc
    33601 gcgggtcatc cccacgccgt tatcggagat ggtcagcgtg cgcttgtctt tatcgaaaga
    33661 gacacgaacg cgcagttcgc catcaccttc gtacaggtcc gggttagata gcgcacggaa
    33721 acgcagcttg tccgccgcat cggaggcgtt agagataagc tcacgcagga agatttcttt
    33781 attggaatag agagaatgga tcatcaggtg cagaagctgt ttcacttctg actgaaaacc
    33841 acgagtttct tgtcctttca tgtaggtcta cctcaataat gccattttaa ggttaaaaac
    33901 aggatgaggg agagatgggg acaagggaga ataatttcaa gcgagagcag gtgctcctgc
    33961 tctcgtttgc ttaaaaacga atcttatgac gcccggcgag ggagtgtgac agcgtggtgc
    34021 catcgaccat ttccagttcg ccgccaaccg gtacgccatg agcgattcgg ctggcttcca
    34081 cgtcatattg cgcgcaaagc tcggcaatgt agttagcggt agcttcacct tcaaccgtgg
    34141 gattggtggc gaggatcact tcagtgattt tttcctctgc cagacgctgt tccagacgat
    34201 caagcccgat atcatccgga ccgatgccgt ccagcggtga cagatgcccc atcaacacaa
    34261 aataacgacc agaaaactgc cccgtctgct caatggcgta gatgtccgcc ggactctcca
    34321 ccacacagat ttgaccgttt tcctgacgac gcggattcga acagatgtta cagacttcct
    34381 gttcggtgaa agtgcggcaa tcggcgcagt ggccgatttc cgacatcgcc cgggtgagcg
    34441 cctgcgccag acgcatcccg ccgctacgat cgcgctgaag cagcgtgaac gccatacgct
    34501 gcgccgactt cgggccaacg cccggcagac agcgcagtgc ttccataagc tgtgttaaca
    34561 gcgggctggt ttgcatcaga acggcatctt aaagccaggc ggcagctgca ttccggagga
    34621 tacagaggcc attttttctt tctgcgtttc ttcaatacga cgtgctgcgt cgttgaatgc
    34681 tgcagccacc aggtcttcca gcatctcttt gtcgtcttcc agcaggctcg ggtcgatctc
    34741 tacgcgacgg cagttgtgtg caccgttgat ggtcactttt accagacctg cgccagattc
    34801 gccggtaact tccagctgcg cgatctcttc ctgcattttc tgcatttttt cttgcatctg
    34861 ctgggcttgc ttcatcaggt tacccagacc gcctttacca aacataggtt tctctctcaa
    34921 tcacgttaag gatgacgaac gtaagctgtg cttacgatca aatggggcgg atactttctt
    34981 catccagctc cgcatcgaaa aaccgacgca gggtctgaat attattatcc gcaataatgg
    35041 actcgcgcgc ctgcgcaagt ttttcttcgt atatcgcctg acgccactcc agcggcgtac
    35101 gcaccgcggg attatcatct tcaacgatag tcagttcaac cgttgaacct tttaacatgc
    35161 tcaacgcttc agccagtttt tgctgtgcac cgcggttgtt caaatgccgc tgagaggagc
    35221 gcaaatgcag acatactgcg ttgtcgctct cctctttcca ggcatttaac gccacctgtt
    35281 cgaccagttt tggtagcgaa agttggctca cctgtgccgc ccacgggtcg cgctcaatgg
    35341 cttctgccgc tagcttcacc gccagttccg gcgttttttc atgttccagc gcttttttca
    35401 gcgccttcgg cgtggcgacc acttcttttt gctgcatcac cggagtggtc gccttccagc
    35461 gatacgcttc ttttttggct ggcgcttttt ccagcgccga tggcaccgga cgcgcctgaa
    35521 cgcgatcggt gaccgaagcc agtctttcca gcgcagcgtt attcaccggc cgcgcgcggg
    35581 tagcggctgc cggttcactc ttttttgctt tggttgctcc ctgcacgcgc tgcaactgct
    35641 ggcgcgccgc cagcacctgg ctggtggttt ccgggagcgg tacagtcggt gcctgctgcg
    35701 gcgctgattg cggcggcacc agggttggcg tcattactgc cgttggcgcg acgggtgcaa
    35761 aggactggcg tggcacttct ggctcaggca gcggcatacg cggatggaac gccagcgcgc
    35821 gcagcagcgt catctcaacg cccatgcggc ggtccggcgc atacggtaat tctttgcgac
    35881 caatcaacag cgtctgatag taaagctgaa tatccgtcgg cggtatggtg cgcgccagtt
    35941 cacgcatccg cagctcgatg gcggccatgt cgttgccaag tgcagcaggc gaaagttgta
    36001 ccatcgcaat acgatgcaac aagccgagca tttccaccag caacgcttcc cactcgatac
    36061 cacgggcagc ggcttcatta atcagtgcca ttacgcgctc gccgttgccc tcgaccatcg
    36121 cttcaaccag cgacagcgcc tgatcgtcgt caagcgtacc cagcatcgca ctgaccgcct
    36181 gggttgaaac ctggccgtca ccgctggcaa tcgcctggtc ggtcagactt aaggcatctc
    36241 gcaggctgcc ttcagcggcg cgcgccaaca attgcagcgc ccgcggctcg tgagcgatat
    36301 gttcttcatt gaggatgtgc tcaagctgat ggcgaatttg ctcgacatcc agcgccttga
    36361 gatgaaattg cagacagcgt gacaaaatcg tcaccggcaa tttctgtgga tcggtcgtcg
    36421 ccagcagaaa cttaacgtgc tccggcggct cttcaagggt ttttaacagt gcgttaaagc
    36481 tgtggcgcga cagcatatgc acttcgtcga tcagataaac tttgaaacga ccacgcgccg
    36541 gagcgtactg gacgttatcc agcaggtcgc gggtatcttc aactttggtg cgcgaggcgg
    36601 cgtcgatttc aatcagatcg acaaagcgcc cctgctcgat ttcacgacag ttatcgcaca
    36661 cgccgcacgg cgtcgcggta atgccggttt cgcagtttag ccccttcgcc agcagtcggg
    36721 cgatagaggt ttttccgacg ccacgggtgc cggaaaaaag ataagcatga tgaatacgcc
    36781 ctaacgacaa gccgttcgcc agtgcggtca gcacatgttc ctggccgacg acgtcagcaa
    36841 aggtttgtgg gcgccatttt cgggctaaaa cctgataact cattggcagg ctctgaaacg
    36901 ctggaaggtg gattcacgaa ggggtaatgc taacacagcc ccgtcagaac ggcgaggctg
    36961 aatgcacatc ttgtgctgtc cgtagcgtgg gcagcacaag actggcgata attaatggcc
    37021 cgggaacggg acaaggctgt agctggtaat gccctgtttt tcgagacgct gttcgccgcc
    37081 gagatcaaac aggttgataa tgaacgcagc gtcagccact tcaccaccca gacgacggat
    37141 cagtttaacg gtcgcttcga tagtgccgcc ggttgccagc agatcgtcca ccaccagaac
    37201 tttgtcgccc ggtttgatgg catcaacgtg gatttccagc tgatcggtgc cgtattccag
    37261 gtcgtaattt tcactgatgg tttcacgcgg cagtttgccc ggtttacgga ccggtacaaa
    37321 gccaacgccc agacccagag ctaccggagc gccaaacaag aagccacgcg cttcggtgcc
    37381 gacaactttg gtaatgcccg catttttgta acgctcaacc agcaagtcga tgctgagagc
    37441 gtaagctttc gggtcttcca gtaagctggt gacatcgcgg aaaagaatgc cgggttttgg
    37501 gtagtcctga atgcttttga tgctattttt gagatactca agctgctgtg cagtcgcggt
    37561 cataagtgta tgcctgcttg ttacggtggt actcacggcg cgttttaaac gtatcaaaag
    37621 taactgttgt ttaacctttg accgcctgcg cctgtgctcg aaaacgccag aatgtactgg
    37681 ctgtacgcaa taattgcaac tgcgattgtt gtgcttcagt gcttttcttg cttttcatca
    37741 atcaccggaa ttcgccacat atagaaaagc aaacaggcga gaattaccag caacatgatg
    37801 cgcacccatg gcatctggac gaaccacaga gaaatggcaa acgtgagcaa aataagcaaa
    37861 atcgcccgcg gtttgacgcc gcgcggcatc gcatgatgtt tctgccagaa acgtagatag
    37921 ctgccaaacc atgagcggta cagcaaccag gcgtgaaagc gcggggaaga acgggcaaag
    37981 caccaggccg ccagcaggat aaacggcgtc gtcggtaata ccggtaatac cacgcccagc
    38041 gtacccagca ctaccgccag ccagccaatg atgattaaaa tgattcgttg catattgagt
    38101 gttgtcagct tacatagtgg ctactttagc ataacaatta tcattttcat tgaggtctta
    38161 tcgtgaaaac cgccctgctg ctggaaaaac tggaaggaca gctcgctacg ctgcgtcagc
    38221 gttgtgcccc ggtgtcacag ttcgccacgc taagtgctcg tttcgacagg catctttttc
    38281 agactcgtgc gacaacacta cagtcttgtc tcgacgaggc gggcgataat ctggctgcgc
    38341 ttcgtcatgc agttgagcag caacagctgc cgcaagtggc ctggctggcg gaacatctgg
    38401 cggcacaact ggaagccatc gcgcgtgaag cctccgcctg gtcattgcgc gagtgggaca
    38461 gtgcgccacc gaaaatttcc cgctggcagc gtaaacgtat tcagcatcag gattttgagc
    38521 ggcggctacg tgagatggtt gccgaacgca gagcccgcct ggcgcgggtg accgatctcg
    38581 tggaacagca aacgctgcat cgtgaagtgg aagcctatga agcgcgcctg gcacgctgcc
    38641 gccatgcgct ggaaaaaatc gaaaacaggc tagcgcgctt aacccgctag caatggaaag
    38701 agtatgtcac ttgaaaatgc ccctgacgat gtcaaactgg ctgtcgattt gattgtgctg
    38761 ctggaggaaa atcagatccc tgccagtacc gtgctgcgcg cgctggatat tgtaaagcgt
    38821 gattatgaaa agaaattaac gcacgatgat gaggcggaaa agtgagaaat aaatggggcc
    38881 gctaaatgtc ccctaagatt gcgggcaaac gcaaaactgc ctgatgcgct acgcttatca
    38941 ggcctacacc gctcctgcaa tatattgaat tcttgcggtt tcgtaggccg gataaggcgg
    39001 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
    39061 tcgccccctt tggttattac ccaaccgctg gtgtcggatc gtcgcctttg tagtcgcgtt
    39121 ttacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
    39181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
    39241 tacggctacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
    39301 caccaaatgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
    39361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
    39421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
    39481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
    39541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
    39601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
    39661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
    39721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
    39781 tagtaatggc atacgacgcg ccctggcgca tattcagtcg cgagagcacc agcacttcca
    39841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
    39901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
    39961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
    40021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
    40081 taacttgctc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
    40141 tcaccagatt ctgccgacgc gccagcgcac gacgccaggc gatacgccgc gccgctacgc
    40201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
    40261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
    40321 tcaatgcaat cgggataatc gacagcacgg taatggtgac tagtcgcatg gtgtgcgact
    40381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
    40441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
    40501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
    40561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
    40621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
    40681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
    40741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
    40801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
    40861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
    40921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
    40981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
    41041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
    41101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
    41161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
    41221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
    41281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
    41341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
    41401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
    41461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
    41521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
    41581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
    41641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
    41701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
    41761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
    41821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
    41881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
    41941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
    42001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
    42061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
    42121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgccgtagc tgaactgtct
    42181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
    42241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
    42301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
    42361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
    42421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
    42481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
    42541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
    42601 cacaggagat acatctccag taagatggcg acgtaatcgc gggcttcttt tttaagatca
    42661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
    42721 attgctgccc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
    42781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
    42841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgt
    42901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
    42961 ggatcgccag ggaattttgc ctgatactca agctctagtt caccaatatt ggattctgac
    43021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
    43081 cgcgtaacgc cagctgcttt tgcaatctcg cccagcgagg tggatgatac cccctgctgt
    43141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
    43201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
    43261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
    43321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
    43381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
    43441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
    43501 ctctgcagat cacaaccgag cttccaggtc gcaccagtgc ctaccggatc gcagaagttc
    43561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
    43621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
    43681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
    43741 atcagaaact gctcggtact cagtacatca gtaagcaagg gtacgatcag gctctggctg
    43801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
    43861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
    43921 tgacggaagg cgcattggta cagaacggtc aggcgactgt gctggcaacc gtgcagcaac
    43981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttccttcgc ctgaaacagg
    44041 aactggcgaa tggcacgctg aaacaagaga acggcaaagc caaagtgtcg ctgatcacca
    44101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
    44161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
    44221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
    44281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
    44341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
    44401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
    44461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
    44521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
    44581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
    44641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
    44701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
    44761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
    44821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
    44881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
    44941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
    45001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
    45061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
    45121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
    45181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
    45241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
    45301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
    45361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
    45421 caacctgctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
    45481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
    45541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
    45601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
    45661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
    45721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
    45781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
    45841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
    45901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
    45961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
    46021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
    46081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttga accgcatgtt cgagaagagc
    46141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctagtg
    46201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
    46261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
    46321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
    46381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
    46441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
    46501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
    46561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
    46621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagctgctt
    46681 gcagaagcag cgaagcaccc tgatatgctg accagcgtac gtccaaacgg tctggaagat
    46741 accccgcagt ttaagattga tatcgaccag gaaaaagcgc aggcgctggg tgtttctatc
    46801 aacgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
    46861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
    46921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc attctcggcg
    46981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa cggcctgcca
    47041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
    47101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
    47161 tatcaggaac gtctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
    47221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ctccgttctc cgttatgctg
    47281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
    47341 gacgtttact tccaagtagg actgctcaca accattgggt tgtcggcgaa gaacgcgata
    47401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
    47461 acgcttgatg cggtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
    47521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
    47581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
    47641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
    47701 catactgtcg atcatcattg atacaacatg taatcactaa ggccgcgtaa gcggcctttt
    47761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
    47821 aaaccagtat taaccacgta taaaattcat aaaaaatact gattgttagt taatttatat
    47881 taagtagcgc taatagattt aataatccat aatcatttag aggctattct taattatttg
    47941 cggtaattct ttattcattc ctcggttatt acgtcatatt cagagcaatc ctggtattag
    48001 tgtcaccaat ttcatctggc gataatcctg aaatgttatg aacagttcga gcaaactgct
    48061 tttacctgct gcgggttagt gctagtatga aaaagtgagt cctgtcccga ttccttccta
    48121 attgtaatgt ttcgtaataa tgcgatgaaa acctgcaaag agtggcttat agttaagcta
    48181 acaaacgaga gggcaagtct aggtcagtaa gttttttcca tcccgaaagg tgtccgttag
    48241 ttcaaccact aagaagggga cgcgttatgg atgaatactc acccaaaaga catgatatcg
    48301 cacagcttaa gtttctctgt gaaaccctgt atcatgactg tcttgcaaac cttgaagaaa
    48361 gcaatcatgg ttgggttaac gacccaacct cggcgatcaa cctccagttg aatgaactga
    48421 ttgagcatat tgcgaccttc gcacttaatt acaaaattaa gtataatgaa gacaataagc
    48481 tcattgagca gatcgacgaa tatctggatg acacctttat gttgttcagc agttatggta
    48541 ttaatatgca ggctcttcag aaatggcgga agtcaggtaa tcgactattc cgttgttttg
    48601 tcaacgcgac gaaagagaat cctgcgagtt tatcttgtta gaattattac aaccatgggt
    48661 agaagtatgt ccgaaaaacc tttaacaaaa accgattatt taatgcgttt acgtcgttgc
    48721 cagacaattg acacgctgga gcgtgttatc gagaaaaata aatacgaatt atcagataat
    48781 gaactggcgg tattttactc agccgcagat caccgcctcg ccgaattgac catgaataaa
    48841 ctgtacgaca agatcccttc ctcagtatgg aaatttattc gctaataaat aattcgcttt
    48901 cggtgctata accggctgtt tattaagaat tttatacttt ttcgccatga agacataccc
    48961 tatgtgatct ttatcacaca gatgtaatga gaacgttctc ttcactgact tttcgtctta
    49021 ctgtgttgcc gcattttcag caaccggagg tcagtaatga gcacagaaaa agaaaagatg
    49081 attgctggtg agttgtatcg ctcggcagat gagacgttat ctcgcgatcg cctgcgcgct
    49141 cgtcagctta ttcaccgata caatcattcc ctggcggaag agcacacatt acgccagtaa
    49201 attctcgcta atttattcgg tcaggtgaca gaggcttata ttgagccaac gtttcgctgt
    49261 gattatggct ataacatttt tctcggcaat aattttttcg ccaacttcga ttgcgtgatg
    49321 cttgatgtct gccctattcg catcggtgat aactgtatgt tggcaccagg cgttcatatc
    49381 tacacggcaa cacatcccat cgaccctgta gcacgtaata gcggtgctga actggggaaa
    49441 cccgtcacca tcggtaataa cgtctggatt ggcggacgcg cggtcattaa cccttgtgtg
    49501 accattggtg ataacgtcgt ggtagcttca ggtgcagttg tcacaaaagg tgtcccggac
    49561 aacgttgtcg tgggcggtaa tccagccaga ataattaaaa aattgtaatc ggtttttcgc
    49621 aactgttatg caaaattgtg gtagatctgt tacttcccct ctactattcc cacgttaaaa
    49681 tagggtgttc cctggaaagt tgcagatacc acgaaggcaa acgatgaccg aaatacaacg
    49741 cctgctgacc gaaacgattg agtctctgaa tacccgcgaa aaacgcgaca acaaaccccg
    49801 ctttagtatc agttttatcc gtaaacatcc ggggctgttt atcggtatgt acgttgcttt
    49861 ttttgccacc ctggcggtga tgttgcagtc cgaaacgctg tcaggctctg tctggctact
    49921 ggttgtatta tttatcctgc ttaatggttt cttctttttc gatgtctacc cacgctaccg
    49981 ctatgaagat atcgacgtgc tggatttccg cgtttgctat aacggcgaat ggtacaacac
    50041 gcgctttgta cctgccgcgc tggttgaagc catcttgaac tctccgcgtg tcgcggatgt
    50101 tcataaggaa caactgcaaa aaatgatcgt ccgtaaaggt gaactgtctt tttacgatat
    50161 ttttaccctc gctcgcgccg aatcaacatc ttaagttagg gttacatacc aggcgtaaag
    50221 ctctgcgcct ggtcaaatga caatgatcgt ttccacccat cacttcatga aataccagct
    50281 ctacctcctt atctcctgcc agcctttttc cacaatcaga tatactttcc ctacactgtg
    50341 ttaataagga tatgctggtg agaacacgac atctggtcgg ccttatttcg ggagtactga
    50401 ttctttcagt attgctgcct gtcggcttaa gcatctggct ggcccatcag caggtagaaa
    50461 catcgtttat tgaagagctg gatacctatt cctcccgcgt cgctattcga gccaataagg
    50521 tggcgacaca agggaaagat gcgctgcagg agctggaaag atggcaaggc gctgcctgta
    50581 gcgaagccca tctcatggaa atgcgtcggg tatcttacag ttatcgctat attcaggaag
    50641 tggtttatat cgataacaac gttccccagt gttcgtctct ggagcatgaa agtccgcccg
    50701 atacctttcc cgagctaggt aaaatttcga aagatggtta tcgtgtctgg ttaacatcgc
    50761 ataacgattt aggcattatc cgttacatgg tcgccatggg aacggcacat tatgtcgtca
    50821 tgatcgaccc cgcttccttt attgatgtca ttccctatag ttcatggcaa attgatgccg
    50881 ccattattgg caatgcccat aacgttgtca taaccagcag cgatgaaatt gctcagggaa
    50941 ttattaccag gctacaaaaa acacccggtg agcatatcga aaataatggg atcatttacg
    51001 atatactgcc cttcctggag atgaatattt cgatcatcac ctgggcttca acgaaaatgt
    51061 tgcagaaagg ctggcatcgg caagtcttta tttggttacc gctcgggttg gtgattggcc
    51121 tgctggcagc gatgtttgtg ctgcgtattt tgcgccgtat ccaatcaccg catcatcggc
    51181 tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta tcagccgatt gtctccttag
    51241 ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg gccgcagaca gacggtagtt
    51301 ggttgtcacc agatagtttt attccgctgg cacagcaaac gggcctttct gagccattga
    51361 cgctactgat tataagaagc gtctttgaag atatgggcga ctgcttgcgt cagcatccac
    51421 agcagcatat ttcgatcaat cttgaatcca ccgtgctcac ctcggaaaaa atcccgcaat
    51481 tgctgcgtga aatgatcaat cactatcagg ttaatcccag acagatcgcg cttgaactca
    51541 ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat aatttctcgc taccgggagg
    51601 cgggccatga aatttatctt gatgattttg gtacggggta ttcaagttta agttatttac
    51661 aggatctgga tgtcgacatt ctgaagatcg ataaatcttt cgttgatgcg ctggaatata
    51721 aaaatgtcac gccgcatatc atcgaaatgg caaaaacact gaaactgaaa atggtagcgg
    51781 agggaatcga aaccagtaaa caagaagagt ggctacgcca gcatggcgtg cactacggtc
    51841 agggctggct ctacagcaag gcattaccga aagaagattt cttacgctgg gccgagcaac
    51901 atttgtgaat ttttgggctg acggcacgcc gcacagcccg tcacgctacc tattcgtcga
    51961 tgattgactc gaatccgcca tagatcattc gcttgccgtc aaacggcatg gactcgccga
    52021 actctttcat ccgtgggtcc gacatcatct tttgattagc tgcgtcgcgg acctcttttg
    52081 aagggtattc aatccagcta aagacaacct cttcattctc ttccgctttc accgccatac
    52141 gaaaatcggt cactttgcca tccggcacat cgctggccca gcattcgaca atacgaagcg
    52201 cgccaaactc tttaaacaat ggcgcggcct tagcggccat ttcccgatag gcatcctttt
    52261 tatcggcagg aacggcaacc acaaaaccat caacatactt catcagacat acctccgaag
    52321 cgtactgcgc agccagacaa ttcttgcgac gtcagttgtg gttaaagttt agccatgctg
    52381 ggattttttg acgaaacggt gggaggcaga aatgggtggg ggccctgcca gctacatccc
    52441 ggcacacgcg tcatctgcct tggctgcttc cttccggacc tgacctggta aacagagtag
    52501 cattgcggga gaaccaacag agcccccatt gagagcgttg aaaaccaacg cgcaggcgca
    52561 ttatcactgc tgccccggct aattgcaacc cattgcgtgc cagatgctgg tttcttgcgc
    52621 aatgcgactt cactcgggcg tttttccaga ttatgctgaa aaattacctc aggaagaaaa
    52681 gatggaaaaa gaagattcat ttccccaacg cgtctggcaa atcgtcgccg ctattcccga
    52741 aggctatgtc accacttacg gtgatgtggc gaaactggcg ggatcgcccc gcgccgcgcg
    52801 ccaggtgggc ggtgtgttaa agcgtctccc tgaaggcagc accttaccct ggcaccgggt
    52861 ggttaatcgc catggcacaa tttcgctaac cgggccggat ttacagcgtc agcgacaggc
    52921 attactggca gaaggtgtga tggtatcggg aagcgggcaa atcgacttgc agcgttatcg
    52981 ctggaactac taaccctctc cgg
//
LOCUS       DAJTNP010000018        52597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTNP010000018 DAJTNP010000000
VERSION     DAJTNP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..354
                     /locus_tag="OO160_001161"
     CDS             <1..354
                     /locus_tag="OO160_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5523364.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO160_001162"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO160_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5523365.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO160_001163"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO160_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5523366.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO160_001164"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO160_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5523367.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO160_001165"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO160_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5523368.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO160_001166"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO160_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5523369.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO160_001167"
     CDS             6877..7281
                     /locus_tag="OO160_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5523370.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO160_001168"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO160_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5523371.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO160_001169"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO160_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5523372.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO160_001170"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO160_001170"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5523373.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO160_001171"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO160_001171"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5523374.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO160_001172"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO160_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO160_001173"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO160_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5523375.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO160_001174"
     CDS             complement(13133..13429)
                     /locus_tag="OO160_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5523376.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO160_001175"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO160_001175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5523377.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPTDFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO160_001176"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO160_001176"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5523378.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO160_001177"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO160_001177"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5523379.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO160_001178"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO160_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5523380.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO160_001179"
     CDS             complement(18686..19372)
                     /locus_tag="OO160_001179"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5523381.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO160_001180"
     CDS             complement(19772..19912)
                     /locus_tag="OO160_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523382.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO160_001181"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO160_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5523383.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO160_001182"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO160_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5523384.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO160_001183"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO160_001183"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5523385.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO160_001184"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO160_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5523386.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO160_001185"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO160_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5523387.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO160_001186"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO160_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5523388.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO160_001187"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO160_001187"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5523389.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO160_001188"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO160_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO160_001189"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO160_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5523390.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO160_001190"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO160_001190"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5523391.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO160_001191"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO160_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5523392.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO160_001192"
     CDS             complement(31511..31678)
                     /locus_tag="OO160_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523393.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO160_001193"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO160_001193"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5523394.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO160_001194"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO160_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5523395.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO160_001195"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO160_001195"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5523396.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO160_001196"
     CDS             35470..36114
                     /locus_tag="OO160_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5523397.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO160_001197"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO160_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO160_001198"
     CDS             37189..37452
                     /locus_tag="OO160_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5523398.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO160_001199"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO160_001199"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5523399.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO160_001200"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO160_001200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5523400.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO160_001201"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO160_001201"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5523401.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO160_001202"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO160_001202"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5523402.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO160_001203"
     CDS             42150..43691
                     /locus_tag="OO160_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5523403.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO160_001204"
     CDS             43663..44526
                     /locus_tag="OO160_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5523404.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO160_001205"
     CDS             complement(44740..45522)
                     /locus_tag="OO160_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5523405.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO160_001206"
     CDS             complement(45519..46592)
                     /locus_tag="OO160_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5523406.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO160_001207"
     CDS             complement(46714..46875)
                     /locus_tag="OO160_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5523407.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO160_001208"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO160_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5523408.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO160_001209"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO160_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5523409.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO160_001210"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO160_001210"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5523410.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO160_001211"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO160_001211"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5523411.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO160_001212"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO160_001212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5523412.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO160_001213"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO160_001213"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5523413.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12528 a        14233 c        13179 g        12657 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatatctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggtggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcaatgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatgttttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagctt gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attacccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatgg
//
LOCUS       DAJTNP010000019        49531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTNP010000019 DAJTNP010000000
VERSION     DAJTNP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO160_001214"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO160_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5523414.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO160_001215"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO160_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5523415.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO160_001216"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO160_001216"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5523416.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO160_001217"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO160_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5523417.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO160_001218"
     CDS             5718..6659
                     /locus_tag="OO160_001218"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5523418.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO160_001219"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO160_001219"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5523419.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO160_001220"
     CDS             7679..8929
                     /locus_tag="OO160_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5523420.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO160_001221"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO160_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO160_001222"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO160_001222"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5523421.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO160_001223"
     CDS             10866..12116
                     /locus_tag="OO160_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5523422.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO160_001224"
     CDS             complement(12172..13260)
                     /locus_tag="OO160_001224"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5523423.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO160_001225"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO160_001225"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5523424.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO160_001226"
     CDS             complement(14194..15243)
                     /locus_tag="OO160_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5523425.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO160_001227"
     CDS             15342..16223
                     /locus_tag="OO160_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5523426.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO160_001228"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO160_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5523427.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO160_001229"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO160_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO160_001230"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO160_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OO160_001231"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OO160_001231"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5523428.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OO160_001232"
     CDS             21991..23067
                     /locus_tag="OO160_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5523429.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OO160_001233"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OO160_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5523430.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OO160_001234"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OO160_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5523431.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OO160_001235"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OO160_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5523432.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OO160_001236"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OO160_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5523433.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO160_001237"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO160_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO160_001238"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO160_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5523434.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OO160_001239"
     CDS             complement(30809..31048)
                     /locus_tag="OO160_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5523435.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO160_001240"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO160_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5523436.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO160_001241"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO160_001241"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5523437.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="OO160_001242"
     CDS             complement(32934..33629)
                     /locus_tag="OO160_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5523438.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="OO160_001243"
     CDS             complement(33626..34024)
                     /locus_tag="OO160_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5523439.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="OO160_001244"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="OO160_001244"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5523440.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO160_001245"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO160_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5523441.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO160_001246"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO160_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37377..38138
                     /locus_tag="OO160_001247"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="OO160_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="OO160_001248"
     CDS             complement(38255..38833)
                     /locus_tag="OO160_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5523442.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="OO160_001249"
     CDS             39003..39590
                     /locus_tag="OO160_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5523443.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="OO160_001250"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="OO160_001250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5523444.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO160_001251"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO160_001251"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5523445.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="OO160_001252"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="OO160_001252"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5523446.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="OO160_001253"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="OO160_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5523447.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="OO160_001254"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="OO160_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5523448.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="OO160_001255"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="OO160_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5523449.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="OO160_001256"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="OO160_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5523450.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="OO160_001257"
     CDS             complement(48911..49018)
                     /locus_tag="OO160_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5523451.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49531)
                     /locus_tag="OO160_001258"
                     /pseudo
     CDS             complement(49078..49531)
                     /locus_tag="OO160_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12205 c        13311 g        12060 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt actagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaaaatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca gggaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc c
//
LOCUS       DAJTNP010000020        49202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTNP010000020 DAJTNP010000000
VERSION     DAJTNP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..736
                     /locus_tag="OO160_001259"
     CDS             <1..736
                     /locus_tag="OO160_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5523452.1"
                     /translation="ACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVD
                     LAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGC
                     LNFFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVT
                     HIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEE
                     THGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            733..1698
                     /gene="yggF"
                     /locus_tag="OO160_001260"
     CDS             733..1698
                     /gene="yggF"
                     /locus_tag="OO160_001260"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5523453.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1720..2229
                     /gene="fumE"
                     /locus_tag="OO160_001261"
     CDS             1720..2229
                     /gene="fumE"
                     /locus_tag="OO160_001261"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5523454.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2226..2939
                     /locus_tag="OO160_001262"
     CDS             2226..2939
                     /locus_tag="OO160_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5523455.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(2911..3588)
                     /locus_tag="OO160_001263"
     CDS             complement(2911..3588)
                     /locus_tag="OO160_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5523456.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3582..4259)
                     /locus_tag="OO160_001264"
     CDS             complement(3582..4259)
                     /locus_tag="OO160_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5523457.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4247..4630)
                     /locus_tag="OO160_001265"
     CDS             complement(4247..4630)
                     /locus_tag="OO160_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5523458.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4646..4954)
                     /locus_tag="OO160_001266"
     CDS             complement(4646..4954)
                     /locus_tag="OO160_001266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523459.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(4955..5533)
                     /locus_tag="OO160_001267"
     CDS             complement(4955..5533)
                     /locus_tag="OO160_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5523460.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5556..5987)
                     /locus_tag="OO160_001268"
                     /pseudo
     CDS             complement(5556..5987)
                     /locus_tag="OO160_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6359..7378
                     /gene="epd"
                     /locus_tag="OO160_001269"
     CDS             6359..7378
                     /gene="epd"
                     /locus_tag="OO160_001269"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5523461.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7428..8591
                     /gene="pgk"
                     /locus_tag="OO160_001270"
     CDS             7428..8591
                     /gene="pgk"
                     /locus_tag="OO160_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5523462.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8806..9885
                     /gene="fbaA"
                     /locus_tag="OO160_001271"
     CDS             8806..9885
                     /gene="fbaA"
                     /locus_tag="OO160_001271"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5523463.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10076..10936
                     /gene="mscS"
                     /locus_tag="OO160_001272"
     CDS             10076..10936
                     /gene="mscS"
                     /locus_tag="OO160_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5523464.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11075..11710
                     /gene="argO"
                     /locus_tag="OO160_001273"
     CDS             11075..11710
                     /gene="argO"
                     /locus_tag="OO160_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5523465.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11803..12543
                     /locus_tag="OO160_001274"
     CDS             11803..12543
                     /locus_tag="OO160_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5523466.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12710..13660
                     /locus_tag="OO160_001275"
     CDS             12710..13660
                     /locus_tag="OO160_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5523467.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13602..15080)
                     /gene="scpC"
                     /locus_tag="OO160_001276"
                     /pseudo
     CDS             complement(13602..15080)
                     /gene="scpC"
                     /locus_tag="OO160_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15104..15889)
                     /gene="scpB"
                     /locus_tag="OO160_001277"
     CDS             complement(15104..15889)
                     /gene="scpB"
                     /locus_tag="OO160_001277"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5523468.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15900..16895)
                     /gene="meaB"
                     /locus_tag="OO160_001278"
     CDS             complement(15900..16895)
                     /gene="meaB"
                     /locus_tag="OO160_001278"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5523469.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16888..19032)
                     /gene="scpA"
                     /locus_tag="OO160_001279"
     CDS             complement(16888..19032)
                     /gene="scpA"
                     /locus_tag="OO160_001279"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5523470.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19236..20129)
                     /gene="argP"
                     /locus_tag="OO160_001280"
     CDS             complement(19236..20129)
                     /gene="argP"
                     /locus_tag="OO160_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5523471.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20512..21171
                     /gene="rpiA"
                     /locus_tag="OO160_001281"
     CDS             20512..21171
                     /gene="rpiA"
                     /locus_tag="OO160_001281"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5523472.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21427..22659
                     /gene="serA"
                     /locus_tag="OO160_001282"
     CDS             21427..22659
                     /gene="serA"
                     /locus_tag="OO160_001282"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5523473.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22888..22947
                     /gene="ibsC"
                     /locus_tag="OO160_001283"
     CDS             22888..22947
                     /gene="ibsC"
                     /locus_tag="OO160_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5523474.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23280..23339
                     /locus_tag="OO160_001284"
     CDS             23280..23339
                     /locus_tag="OO160_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5523475.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23440..24042)
                     /locus_tag="OO160_001285"
     CDS             complement(23440..24042)
                     /locus_tag="OO160_001285"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5523476.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24063..24246)
                     /gene="ssrS"
                     /locus_tag="OO160_001286"
     ncRNA           complement(24063..24246)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO160_001286"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24288..24617)
                     /gene="zapA"
                     /locus_tag="OO160_001287"
     CDS             complement(24288..24617)
                     /gene="zapA"
                     /locus_tag="OO160_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5523477.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24785..25363
                     /gene="ygfB"
                     /locus_tag="OO160_001288"
     CDS             24785..25363
                     /gene="ygfB"
                     /locus_tag="OO160_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5523478.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25389..26714
                     /gene="pepP"
                     /locus_tag="OO160_001289"
     CDS             25389..26714
                     /gene="pepP"
                     /locus_tag="OO160_001289"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5523479.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26711..27889
                     /gene="ubiH"
                     /locus_tag="OO160_001290"
                     /gene_synonym="visB"
     CDS             26711..27889
                     /gene="ubiH"
                     /locus_tag="OO160_001290"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5523480.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            27912..29114
                     /gene="ubiI"
                     /locus_tag="OO160_001291"
                     /gene_synonym="visC"
     CDS             27912..29114
                     /gene="ubiI"
                     /locus_tag="OO160_001291"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5523481.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29188..29271)
                     /locus_tag="OO160_001292"
     CDS             complement(29188..29271)
                     /locus_tag="OO160_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523482.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29562..30656
                     /gene="gcvT"
                     /locus_tag="OO160_001293"
     CDS             29562..30656
                     /gene="gcvT"
                     /locus_tag="OO160_001293"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5523483.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30680..31069
                     /gene="gcvH"
                     /locus_tag="OO160_001294"
     CDS             30680..31069
                     /gene="gcvH"
                     /locus_tag="OO160_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5523484.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31188..34061
                     /gene="gcvP"
                     /locus_tag="OO160_001295"
     CDS             31188..34061
                     /gene="gcvP"
                     /locus_tag="OO160_001295"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5523485.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34328..35071
                     /locus_tag="OO160_001296"
     CDS             34328..35071
                     /locus_tag="OO160_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5523486.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35128..36561)
                     /gene="bglA"
                     /locus_tag="OO160_001297"
     CDS             complement(35128..36561)
                     /gene="bglA"
                     /locus_tag="OO160_001297"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5523487.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36606..36916
                     /locus_tag="OO160_001298"
                     /pseudo
     CDS             36606..36916
                     /locus_tag="OO160_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37080..37739
                     /locus_tag="OO160_001299"
     CDS             37080..37739
                     /locus_tag="OO160_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5523488.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37935..38915)
                     /gene="ygfZ"
                     /locus_tag="OO160_001300"
     CDS             complement(37935..38915)
                     /gene="ygfZ"
                     /locus_tag="OO160_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5523489.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39158..39424
                     /gene="sdhE"
                     /locus_tag="OO160_001301"
     CDS             39158..39424
                     /gene="sdhE"
                     /locus_tag="OO160_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5523490.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39405..39812
                     /locus_tag="OO160_001302"
     CDS             39405..39812
                     /locus_tag="OO160_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5523491.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39852..40373)
                     /gene="fldB"
                     /locus_tag="OO160_001303"
     CDS             complement(39852..40373)
                     /gene="fldB"
                     /locus_tag="OO160_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5523492.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40485..41381
                     /gene="xerD"
                     /locus_tag="OO160_001304"
     CDS             40485..41381
                     /gene="xerD"
                     /locus_tag="OO160_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5523493.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41406..42116
                     /gene="dsbC"
                     /locus_tag="OO160_001305"
     CDS             41406..42116
                     /gene="dsbC"
                     /locus_tag="OO160_001305"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5523494.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42122..43855
                     /gene="recJ"
                     /locus_tag="OO160_001306"
     CDS             42122..43855
                     /gene="recJ"
                     /locus_tag="OO160_001306"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5523495.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            43946..45044
                     /gene="prfB"
                     /locus_tag="OO160_001307"
     CDS             join(43946..44020,44022..45044)
                     /gene="prfB"
                     /locus_tag="OO160_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5523496.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45054..46571
                     /gene="lysS"
                     /locus_tag="OO160_001308"
     CDS             45054..46571
                     /gene="lysS"
                     /locus_tag="OO160_001308"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5523497.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46614..47162)
                     /gene="idi"
                     /locus_tag="OO160_001309"
     CDS             complement(46614..47162)
                     /gene="idi"
                     /locus_tag="OO160_001309"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5523498.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47217..47288
                     /locus_tag="OO160_001310"
     CDS             47217..47288
                     /locus_tag="OO160_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523499.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47285..47410)
                     /locus_tag="OO160_001311"
     CDS             complement(47285..47410)
                     /locus_tag="OO160_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523500.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47412..48860)
                     /gene="uacT"
                     /locus_tag="OO160_001312"
     CDS             complement(47412..48860)
                     /gene="uacT"
                     /locus_tag="OO160_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5523501.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11878 a        12324 c        13257 g        11743 t
ORIGIN      
        1 ggcgtgtgcg ggtccgatgg gcattggcgc tatcgattac gccattaacg gtggcataca
       61 accgtcacgg gtggtggtgg tcgatatcga cgacaaacgc ctggcgcagg tgcagaagct
      121 gctgccggtg gatctggcgg ccagtaaagg cattgagctg gtgtatgtga ataccaaagg
      181 gatgagcgat cctatccaga tgctgcgggc gctgacagga gatgtcgggt tcgatgacat
      241 ttttgtttat gcggcggtgc ctgctgtcgt tgagatggct gacgaattac tggcggaaga
      301 tggctgtctg aacttctttg ccgggctgac ggataaaaac ttcaaagtgc cgtttaattt
      361 ctacaacgtc cattacaaca gcacgcacgt agtcggtaca tccggcggtt caacggacga
      421 catgaaagag gcgattgccc ttagcgccac aggacagtta cagccgtcgt ttatggtgac
      481 ccatattggt ggcctggatg cggtgccaga aaccgtgctc aatctgccgg atatccctgg
      541 cggtaaaaaa ctcatttata acggcgtgac catgccgctc actgccattg ccgattttgc
      601 cgaaaaaggc aaaaccgatc cgctgtttaa agagttggcg cggctggttg aggaaacgca
      661 cggcatctgg aatgaacagg ccgagaaata tctgctggca caatttggcg ttgatatcgg
      721 ggaggccgcg caatgatgtc cctggcgtgg ccattatttc gcgttacgga acaggcagcg
      781 ttggctgcct ggccgcaaac cggatgtggc gacaaaaata aaattgatgg cctggcggtc
      841 accgcgatgc gccaggcatt aaacgacgtc gcttttcgtg gacgagtggt tatcggcgaa
      901 ggagaaattg accatgcgcc gatgctgtgg attggcgaag aggtgggcaa aggcgatggg
      961 ccagaagtcg atatcgcggt tgatcccatt gaaggcacgc ggatggtggc gatggggcag
     1021 agcaatgcgc tggcggtcat ggctttcgct cctcgagata gcctgttaca tgcgcccgat
     1081 atgtatatga aaaagctggt tgttaatcga ctggctgccg gggcgattga tctgtctttg
     1141 cctctggcgg acaacttgcg taacgtggcg cgggcgttag gtaaaccgct ggacaagctg
     1201 cgcatggtta cgctggataa accgcgtctg agtgccgcga ttgaagaagc aacacagctg
     1261 ggcgtgaagg tttttgccct gccggatggc gatgtcgccg ccagcgtgct gacctgctgg
     1321 caggataatc cttacgatgt gatgtacacc atcggcggtg cgccagaagg cgtgatttct
     1381 gcctgtgccg ttaaagcgtt aggcggggat atgcaggccg agctgattga cttttgtcag
     1441 gctaaagggg attacacgga aaatcggcag attgcagagc aggagcgcaa gcgttgtaag
     1501 gcaatgggcg ttgacgttaa ccgtgtgtac tcgctcgatg aactggtgag ggggaacgat
     1561 atcctcttta gcgctacggg cgtgacgggc ggcgagttgg tgaacggtat ccaacagacg
     1621 gcgaatgggg tgcggacgca gacattactg atcggcggcg cggaccaaac gtgtaatata
     1681 atagactccc tgcattgatg gtgatctatc ggaagaccaa tggcgacgct gacagaagat
     1741 gatgtgcttg agcaactgga tgcacaggac aatttatttt catttatgaa aactgcgcat
     1801 tctattttgc tccaggggat acgccagttt ctgccgtcgc tgtttgtcga taacgatgaa
     1861 gagatcgtcg aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct tgacgatatt
     1921 gatgttgcgc tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct gtatgccgat
     1981 atcacgcact tttcccagta ctggcattac ctgaacgaac aggatgaaac gcccggattt
     2041 gccgatgata taacctggga ttttatcagc aatgtcaaca gtataactcg caatgcgacg
     2101 ctctacgatg cgttaaaagc gatgaagttc gccgatttcg ccgtctggtc agaggcgcgt
     2161 tttagcggaa tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt aaaggaatta
     2221 acgccgtgaa aattgaatta acggtgaatg ggctgaacgt gcaagcgcag taccatgatg
     2281 aagagataga acgcgtgcat aaactgttgc tgcgtatgct ggcggcctta cagacggtaa
     2341 atccgcagcg gcgtacggtg gtttttcttt gcgctccgcc ggggacgggt aaatctaccc
     2401 taaccacctt ctgggaatat ctcgcgcaac aagacccgga gctgcccgct attcaggcgc
     2461 tcccgatgga tggttttcac cattacaata gctggctgga cgcgcatcaa ttgcgcccct
     2521 tcaaaggcgc accagagaca tttgacgttg cgaaactggc ggaaaatctg cgccaggtgg
     2581 tggaagggga ttgtacgtgg ccgcagtacg atcgacaaaa gcatgatcct gttgaagatg
     2641 cgttgcacgt taccgcacca ctcgtcatcg tcgaaggaaa ctggttgttg ctggacgacg
     2701 agaagtggtg ccagcttgcg caattttgtg atttctcgat ctttatcaac gcgccagctg
     2761 ctgcgttgcg ggagcgtctg gtggggcgca agctggcggg aggggtatcg ctggcagatg
     2821 cagaagcctt ttatgaccgt accgacgggc cgaatgttcg ccgggggctg gaagagagtc
     2881 tgcctgcaaa tttgacctta atgatgaccg ctacgggtga atatcgtctg gtggattaac
     2941 gttcctgatt acaccatctt ccagccgcac cacacgagag aaatgacgcc gggtaaatgc
     3001 ggcgtcatga ctgattgcta ccacgctcgt gcctcgctgg cggcacttct ccagccagct
     3061 ttcaaacacg ctcaaccaat tttcgtcaaa atcccgactc ggctcgtcca gcagtaatag
     3121 tggcggagag agtgcttcca ggcaggcgac ggcgaccatc cgtcgctgcg cattatgcag
     3181 atccaacggg tgcgagttgg cagtgtctgc taactgacaa cattgcaggg cagcatgggt
     3241 acgttgagtg atttcatccg ccgggcattt ttgcagtttc aaaccaaatg cgatctcgtc
     3301 agcgacggtg ctgtgaaaaa gttggttttc cgcttcctga aacagtacgc caacctttgc
     3361 cgcgcgctgg cggtttttca ggtttttcat cgcctgttgt tgcagcataa ccgtaccggc
     3421 tgtgggcgta agaagacctg ccattactcg taacagcgtc gatttccctg cgccattgtc
     3481 accggtcagc gccaaccatt caccttgttt gagttgcaga gaaatgtcgc aaagacaatc
     3541 cgttgccgcg ccaggccagc gataagatat cttatttaac gttaacatag tggctggagc
     3601 gctccgtttt gcaattgcca cgcttgctgg cagtagttca ggaaaggtgt gcgatgacgt
     3661 tcaaaaagaa taatcagcga accacgttca aacgcccagt gttgtaagcg ctgcaacagc
     3721 atttcgctgg cctgcggtgt caggcggcta aaggcttcat caagaattaa gagctttggc
     3781 tgcatcgcga tggcgcaggc gatgatcacg cgctgtgtct ctccaccgga gagtgttgcc
     3841 gggtgacgat ggcgcaacgg ctggcactcg gtcagagtca gggcggcatc aatccgcgcc
     3901 ataatctctg cttcagcaag acacagattt tctggcccaa aggcaacttc ttcttccacg
     3961 ctaaaggtac agccggagag ctgcaaatag ggcgattgct ggacgagctg gatggtggcg
     4021 gattgttcat taagcgggag atgcccaatg ggcgtaccga ggagcgtccc cgtgcctgtt
     4081 atctctcttg gcagaaagtc cggataccag cccgccatca gctgtgccag cgtacttttg
     4141 ccactgccgt tgtcaccaaa gatggcgacc atccccggtt tgacatagtg aaagtcataa
     4201 cagaacggag gatgcgttga gtgtgtcggg caataacgga actgttctaa cgtaaccata
     4261 tccacgctcc gaattctgtc agcatcagca agatcatggt gtaacgcgct acgcgttgta
     4321 gggtgctatc ggctggtgcc cacaacgttg ttcgtcggtt gtggatccga aatgcccgca
     4381 tatccagcgc cgcaccacgg acagccagat cgttaagcgc attgtgtgtt agcggaataa
     4441 ttaacgcggg catagcacgt aaacgctgat accagccctc atccagcggc acgccgcggg
     4501 cacgctgcgc ttcatgaata atcgctaatt gccgttttaa ctgctcaacg accaataacg
     4561 gcccggcaaa cagataagcg acgcctggcg gcaggcgaga agcaaacaga gcgcgaataa
     4621 aacattgcac ggggacgtac tgcattcaca actgtgaggt tgaaacaatc gccagaatca
     4681 gcaaccagag cgtaatagca tgcgaccacc gttctggcga gcgtggcgtg ccgcttagcc
     4741 attctggtag ccagccaccg tgtaccagcc acagcccggc tcccagcgag aacatcagcc
     4801 agacgacata ttttgcccgc cgccgcgtgg cctttagcgc aataaggcag aaaaaggtcg
     4861 ccgcgctata gatcggcaaa atggtctgcg cgggcaggat aagcgtggtg caggccgcta
     4921 atgcccacag tgttaatgag gtaaatggat gcatttagcg cacggcagcc attgccggaa
     4981 aatggcgcgt ggtgcgaatc ggcagctggc gcagtaaaag ccagacaatc accgctgtaa
     5041 gaattttatc gaccaggttc gcgcctatca ccgtaatcgc caccgactcc accaggtttt
     5101 gccccatcga gtgcatccag gcaacgaaca aatcggctcc gctaccggtg acaccgccga
     5161 acagcgcagt acgcagcggt acagcgacga ccgttaccgc aagcgtaata atcacgccgc
     5221 tgacaacgac ttttggcagg gtgcgaaacc aacccgctcg cgccagccaa ccggagacca
     5281 ggccgatgac catcgcgacg ggtgcaaatg cggcggcgat cgggtcggtc agcaatcccc
     5341 agagtaaatt ggtcagtaag ccggtcagca ttccgatcac cggaccaagc agtaccgcgc
     5401 taataagtgt gccgatggag tcgagaaata taggtaattt gaccatgctg gccagttgcc
     5461 cgccgatcat attgatggcg atagatataa cgatcaacac cagtgcctga ctggaaaagt
     5521 ggctacgcgc cataagaaaa cctctttgaa agtagttatt taacggggga aatatgcaac
     5581 tcgtcgtaac cagaaagcgc gcaaggttgg cggctaaacg ccagttcctc aaggctaccg
     5641 atcaccagtt caagcgtggt ccgcacggta cagccgggca tcatatgacc gccaagcatc
     5701 gtgccgtgtg gatcggaaac gcagagatga agatgctcac cactctgttc cagcgtgccg
     5761 tttagtgaga tcacttcgaa tgtaccgttc aggagcgtgg tgccttcttg cccggcatag
     5821 cgcaaggcga cgtcagtcag gctaccggta cagcctgcaa tctaggcggc atgaagttga
     5881 tgttgctgtg caaaagcgtg tagctgggaa aaaacttcct gcccggggag gaggcgcagt
     5941 gcgtaaaagc gtgcggagga agaatatgag tagggcaatg tcatcattgg agtactccga
     6001 aactcggacg tattgcggat aatatcgacg ctgcatcagt catattctga tataagtcag
     6061 ggtttgtgac ggcacaacga aaagattttg ctacgcttat tcgtgattct gcaaagtgga
     6121 ataaattcca caggggcggg gctgaaaacg tgacgtaagt cagataattg ctttctttga
     6181 ctggacaatc attcctttta ttccacgttt cgcttatcct agctgaagcg tttcagtcga
     6241 ttaaatgttc gacaattaac caatcagtcg cagtttgcga caggtaaggt ttccccggac
     6301 gatttgctgg attactctgt ctggcaaatt tgctcaggga aaaccttgca ggagatctat
     6361 gaccgtacgc gtagcgataa atggcttcgg tcgcatcggg cgtaatgtgg ttcgtgcttt
     6421 gtatgaatcc ggacgccggg cggaaattac cgtggtggca atcaacgaac tggcggatgc
     6481 tgcgggcatg gcgcatttgt tgaaatatga caccagccat ggccgttttg catgggaagt
     6541 acgacaggaa cgcgatcaac tttttgttgg tgatgacgcc atccgcgtat tgcatgaacg
     6601 ttcactgcaa tcgctcccct ggcgtgaact tggcgttgat gtagtcctcg actgcaccgg
     6661 cgtatatggc tcccgcgagc atggcgaagc gcatattgcc gccggggcca aaaaagtgct
     6721 cttttcacat cctggcagta acgatctcga cgcgaccgtt gtttacggcg tcaatcagga
     6781 tcaacttcgt gcggaacacc gcatcgtttc taacgcttcc tgtaccacga attgcataat
     6841 tcccgtcatc aaattgttag atgatgcgta cggtattgag tccggcactg tgaccacaat
     6901 tcactccgcc atgcacgatc aacaggttat tgatgcatac catcctgacc tgcgtcgcac
     6961 ccgggcagcc agccagtcga tcattccggt cgatactaaa ctggccgctg gtatcacacg
     7021 attttttccg caatttaacg atcgctttga agcgattgcg gtacgtgtgc caaccataaa
     7081 tgtgacggca atcgatttaa gcgtgacggt gaagaaacct gtaaaagcca atgaagtcaa
     7141 cctgttgctg caaaaagcag cacaaggtgc atttcatggt atagttgact atacggaatt
     7201 gccgttggtc tctgtagatt ttaaccacga tccgcacagt gccattgtcg atggcaccca
     7261 aacccgggtc agtggcgcac acctgatcaa aacgttggtc tggtgcgata acgaatgggg
     7321 ctttgctaac cgaatgctcg acacgacgtt agctatggct actgttgctt tcaggtaaga
     7381 cgcaagcagc gtctgcaaaa cttttagaat caacgagagg attcaccatg tctgtaatta
     7441 agatgaccga tctggatctt gctgggaaac gtgtatttat ccgtgcggat ctgaacgtac
     7501 cagtaaaaga cgggaaagta accagcgacg cgcgtatccg tgcttctctg ccgaccatcg
     7561 aactggccct gaaacaaggc gcaaaagtga tggtaacttc ccacctgggt cgtcctaccg
     7621 aaggcgagta caacgaagaa ttctctctgc tgccggttgt taactacctg aaagacaaac
     7681 tgtctaaccc ggttcgtctg gttaaagatt acctcgacgg cgttgacgtt gctgaaggtg
     7741 aactggttgt tctggaaaac gttcgcttca acaaaggcga gaagaaagac gacgaaaccc
     7801 tgtccaaaaa atacgctgca ctgtgtgacg tgttcgtaat ggacgcattc ggtactgctc
     7861 accgcgcgca ggcttctact cacggtatcg gtaaattcgc tgacgttgcg tgcgcaggcc
     7921 cgctgctggc agctgaactg gacgcgctgg gtaaagcact gaaagaacct gctcgcccga
     7981 tggtggctat cgttggtggt tctaaagtat ctaccaaact gaccgttctg gactccctgt
     8041 ctaaaatcgc tgaccagctg attgttggtg gtggtatcgc taacaccttt atcgcggcac
     8101 aaggccacga tgtgggtaaa tccctgtacg aagctgacct ggttgacgaa gctaaacgtc
     8161 tgctgaccac ctgcaacatc ccggttccgt ctgatgttcg cgtagcaacc gagttctctg
     8221 aaactgcacc ggctaccctg aaatctgtta acgatgtgaa agctgacgag cagatcctgg
     8281 atatcggtga tgcttccgct caggaactgg ctgaaatcct gaagaatgcg aaaaccattc
     8341 tgtggaacgg tccggttggc gtgttcgaat tcccgaactt ccgcaaaggt actgaaatcg
     8401 tggctaacgc tatcgcagac agcgaagcgt tctccatcgc tggcggcggc gacactctgg
     8461 cagcaatcga cctgttcggc attgctgaca aaatctccta catctccact ggcggcggcg
     8521 cattcctcga attcgtggaa ggtaaagtac tgcctgcagt agcgatgctc gaagagcgcg
     8581 ctaagaagta aaaaatcaca gggcagggaa acctgcactt gtttcagcgc gcttttagag
     8641 cacgcatttt ttcatactct aaataattcg agttgcagga aggcgacaag agagtgaatc
     8701 cccaggagct tacataagta agtgactggg gtgagcgaac gcagacgcag cacatgcaac
     8761 ttgaagtatt atgagtataa ggcccgacga tacaggacaa gagacatgtc taagattttt
     8821 gatttcgtaa aacctggcgt aatcactggt gatgacgtac agaaagtttt ccaggtagca
     8881 aaagaaaaca acttcgcact gccagcagta aactgcgtcg gtactgactc catcaacgcc
     8941 gtactggaaa ctgctgctaa agttaaagcg ccggttatcg ttcagttctc caacggtggt
     9001 gcttccttta tcgctggtaa aggcgtgaaa tctgacgttc cgcagggtgc tgctatcctg
     9061 ggcgcgatct ctggtgcgca tcacgttcac cagatggctg aacattatgg tgttccggtt
     9121 atcctgcaca ctgaccactg cgcgaagaaa ctgctgccgt ggatcgacgg tctgctggac
     9181 gcgggtgaaa aacacttcgc agctaccggt aagccgctgt tctcttctca catgatcgac
     9241 ctgtctgaag aatctctgca agagaacatc gaaatctgct ctaaatacct ggagcgtatg
     9301 tccaaaatcg gcatgactct ggaaatcgaa ctgggttgca ccggtggtga agaagacggc
     9361 gtggacaaca gccacatgga cgcttctgca ctgtacaccc agccggaaga cgttgattac
     9421 gcatacaccg agctgagcaa aatcagcccg cgtttcacca tcgctgcgtc cttcggtaac
     9481 gtacacggtg tttacaagcc gggtaacgtg gttctgactc cgaccatcct gcgtgattct
     9541 caggaatatg tttctaagaa acacaacctg ccgcacaaca gcctgaactt cgtattccac
     9601 ggtggttccg gttctactgc tcaggaaatc aaagactccg taagctacgg cgtagtgaaa
     9661 atgaacatcg ataccgatac ccaatgggca acctgggaag gcgttctgaa ctactacaaa
     9721 gcgaacgaag cttatctgca gggtcagctg ggtaacccga aaggcgaaga tcagccgaac
     9781 aagaaatact acgatccgcg cgtatggctg cgtgccggtc agacttcgat gatcgctcgt
     9841 ctggaaaaag cattccagga actgaacgcg atcgacgttc tgtaagtcat tcctttctgc
     9901 ttatctcaag gcccgctctg cgggtctttt tttcgccaaa agcaaggtga ttcagatgag
     9961 aaatctgtga tctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt
    10021 cataaggcgg cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga
    10081 agatttgaat gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc
    10141 gctgctgcta agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt
    10201 gattatcgcg cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga
    10261 tgccactgtt gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct
    10321 aatcgctgcc ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc
    10381 cgcaggctta gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt
    10441 gttacttgtc atgttccgcc cgttccgtgc cggagaatat gttgacctcg gcggcgtagc
    10501 cggtactgtg ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat
    10561 tatcgttatt ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc
    10621 agttcgccgt aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa
    10681 gcagatcctg accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac
    10741 tgtgcgcctg aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa
    10801 cagcggcgat ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga
    10861 tgccgccggt atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga
    10921 agacaaagct gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta
    10981 gttattctga ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt
    11041 ctctgcatca gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct
    11101 tgctcttggg gcagctatga ttctgccgct cggcccacaa aatgcttttg tgatgaatca
    11161 gggcattcgt cgtcagtacc acattatgat tgccttactt tgcgctatca gcgatttggt
    11221 cctgatttgc gcggggattt ttggtggtag cgcgttattg atgcagtcgc cgtggttgct
    11281 agcgctggtc acctggggcg gcgtagtctt cttgctctgg tatggttttg gcgcttttaa
    11341 aacagcgatg agtagtaata ttgagttagc tagtgccgaa gtcctgaagc aaggcagatg
    11401 gaaaattatc gccaccatgt tggcagtgac ctggctgaat ccgcatgttt acctggatac
    11461 ttttgttgta ctgggcagcc ttggcgggca gcttgacgtc gaaccaaaac gttggtttgc
    11521 gctcgggaca attagcgcct ctttcctgtg gttctttggt ctggctattc tcgcagcctg
    11581 gctggcaccg cgcctgcgca cggcaaaatc acagcgcatt atcaatctgg tagtgggatg
    11641 tgtcatgtgg tttatagcct tgcagctggc gagagacggt attgctcatg cacaagcctt
    11701 gttcagttag gcgctatctg atggaaaaat aaaacagagg cgctaagctt gcctccagag
    11761 gtcctgaatt ttgggcaagt caattaacac ggagagacta acgtgaagtt caaagttatc
    11821 gccctggcgg cattaatggg tattagcggg atggcagcgc aggctaacga attgccggat
    11881 ggaccgcata ttgtcacctc cggtacggca agtgtggatg cggtgccaga cattgccact
    11941 cttgcgattg aagttaacgt ggccgcgaag gatgccgcta cagccaagaa acaggcagat
    12001 gagcgcgtcg cacaatacat ttccttcctt gaactcaatc agatcgcgaa aaaagatatc
    12061 agctcagcga atttacgcac ccagccagat tatgactatc aggatggtaa aagtatcctg
    12121 aaaggctacc gagctgtgag aacggtggaa gtcacgctcc gtcagttaga caaactgaat
    12181 tccttgctgg atggcgcgct gaaggcgggt cttaacgaaa ttcgttctgt gtcgctgggc
    12241 gtggcgcagc cggatgccta taaagacaaa gcgcgtaagg cagcgattga taacgcgatt
    12301 catcaggcgc aggaactggc gaacggcttt catcgtaaac tggggccggt atatagcgtg
    12361 cgctaccatg tttccaacta tcagcccagc ccaatggtgc ggatgatgaa agccgatgcc
    12421 gcgccggtgt ctgcccagga aacttacgag caggccgcta ttcagtttga tgatcaggtc
    12481 gatgtggtct tccagttaga acctgtggat caacaacccg ctaaaacacc tgcagcacaa
    12541 taatacttgt agcccgacgt attcgtcggg ctaatgcctt tacccgatat tgcctccttt
    12601 ttctgtaatt ttctgtaaaa ccatgacgta atcacctatc tttgctcgcg gtttcatcaa
    12661 ggataggctg actgaaacgg ttaagcggac aaggatgcgg ggagggggga tggacatttt
    12721 tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata ttgcccgggc
    12781 ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg cgctggaaga
    12841 acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca ataaagccgg
    12901 tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga ttgataatga
    12961 aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata atacttatcc
    13021 gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg tgaccgccca
    13081 gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc agttagattt
    13141 tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc tgactatcag
    13201 tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag agcgtcaaga
    13261 gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc gggctaactt
    13321 cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca ttatttatcg
    13381 tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc tgacggtgat
    13441 cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg atgcgccgca
    13501 caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt ataccgccac
    13561 gatcttcagc atattgctga agatcgtgac gggacaaacc attaacccag catcgagccg
    13621 gttgcaatta aattacggtg taagtcgaag acgtggctaa gatcgtggtg aatatgtccg
    13681 ccgggcgcat tttccagata gcgatgcaga taatcccgat acataggatg tgcacaatta
    13741 tcaatgatag tgcgggcgcg ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt
    13801 tcggtgatga tcactttgac gctgtgttcg ctgtgatcaa catggctgca cattggcacg
    13861 acggttgaga tcttgccttc tttagcaatc gacggagcca taaagatcga cagatacgcg
    13921 ttgcgttcaa aatcaccgct gccgccgatg ccgttcatca gatcgacccc ggctacgtgc
    13981 gttgaatttg catgcccgta aatatcaaac tccaggccga cgttcagagc gatgacgccc
    14041 agacgacgga tgatttccgg gttattggaa atctcctgcg gacgcaacac aatgcggctg
    14101 gcaaagtaat ccatattgtc gtaaatcttg cgcagggaat cggccgagat tgtcaggctg
    14161 gaggcgctgg ccccgctgat tttgccggtt tccagtaaat gcaccaccga ttcctgtagc
    14221 acttccgaat acatcatgaa cggaggaatt tccgggtttt cccccagacg tgccattacc
    14281 gcattattga tattgcccac gccactttgc agcggcagaa attccggcgg aatacgccca
    14341 tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg gcacatggga
    14401 ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac ggcgacaatc
    14461 tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg gaagatcgac
    14521 acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc tgcaacgcgc
    14581 ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag ctaggtcggc
    14641 gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc cgatgcttca
    14701 atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc
    14761 aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa actggacgat
    14821 gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg
    14881 gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc ctcatgctgt
    14941 tcgttagctc tgcgggcaat cgcggcgggt agggctttcg gcgaacccgc tggggtaaag
    15001 ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc ggcggtcatc
    15061 cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga ccaacgaaat
    15121 taggtttacg tttttcgagg aaagcgttca tcccttcctg gtaatcttcg ctgtcataaa
    15181 ccgcgcggcg catcccctga atacgttcaa attcatcgga gttcatggtg tgtgcttcgc
    15241 ccagtacacg cagctcttct ttgataacgg caatggctaa cggcgctttc tcagagatgt
    15301 ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca tggttgagga
    15361 tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agctctttga
    15421 caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc
    15481 cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac
    15541 tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt
    15601 tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat
    15661 cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggatccac
    15721 ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt aacgcctgca
    15781 taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag ttaaactcaa
    15841 tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata aaaattcctt
    15901 taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag
    15961 agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa atcttcattc
    16021 gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt
    16081 tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg
    16141 gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag accccgtggc
    16201 tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca
    16261 atggcgacat tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact
    16321 tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag
    16381 ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgatgcc aaccgtttcg
    16441 acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg tgctcgctga
    16501 ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc
    16561 gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg
    16621 ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat gccaaaggct
    16681 tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta
    16741 ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg acgtgggtga
    16801 cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg acgtaagcgg
    16861 cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac
    16921 gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc
    16981 ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc
    17041 gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc
    17101 cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gctaggcggg cgatctcttc
    17161 aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc
    17221 gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg
    17281 gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga
    17341 ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg
    17401 caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg
    17461 aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt
    17521 cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag
    17581 cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc
    17641 ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag
    17701 cgactgttcg cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc
    17761 tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat
    17821 ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgtggcagag
    17881 ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt
    17941 aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt
    18001 cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag
    18061 gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc
    18121 aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat
    18181 aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt catcaatttt
    18241 cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgagcg taaatgctac
    18301 ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt
    18361 aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg
    18421 ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat cgttttgaat
    18481 ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc
    18541 cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat
    18601 ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac
    18661 gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc
    18721 aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt
    18781 tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    18841 acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc
    18901 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    18961 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    19021 ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    19081 tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc
    19141 cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa
    19201 cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga
    19261 ccataatcga gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg
    19321 tgccagtaga gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg
    19381 gccagctctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca
    19441 agttgtacga acgcttctga agaattaacg atatggcagg gtacgctgcc aggaggaaga
    19501 tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact
    19561 ggcgctttca ataatgccga acgcgttacg ccgttaggaa aatatttttc ggcaaacggt
    19621 tttgagctga cgaacagata gtcgagcgca ccgagtttat cgacaagaca actcggcagc
    19681 gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta
    19741 cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc
    19801 agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc
    19861 ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc
    19921 agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac
    19981 agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta
    20041 atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc
    20101 tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt
    20161 cctgcactat gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca
    20221 tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg
    20281 cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct ctgttcgcac
    20341 cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg
    20401 ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg gtctgaatcg
    20461 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    20521 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggtaccatt
    20581 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg cacgatgaaa
    20641 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    20701 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    20761 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    20821 aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc caagcaggtt
    20881 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    20941 gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    21001 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    21061 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    21121 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    21181 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    21241 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    21301 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    21361 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    21421 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    21481 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    21541 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    21601 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    21661 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    21721 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    21781 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    21841 tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    21901 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    21961 ttttatgata ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct
    22021 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    22081 aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc
    22141 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    22201 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    22261 tctccactgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    22321 gaagcgcagg agaatatcgg cttggaagtt gcgggtaaat tgatcaagta ttctgacaat
    22381 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt
    22441 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    22501 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    22561 gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    22621 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa
    22681 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    22741 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    22801 tatttaatgg ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc
    22861 gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    22921 ttactgataa gtttcggagc ttattaacag tcagtctcaa gggaggagtc atcctccctt
    22981 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg
    23041 catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata
    23101 gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga
    23161 aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg
    23221 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    23281 tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac
    23341 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    23401 atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga
    23461 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    23521 ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt
    23581 ccgatcataa aaaccaccgc ccattcccag acgctgaccg tactcatcaa aggcgaccag
    23641 cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg
    23701 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    23761 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    23821 gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac
    23881 cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    23941 ttccagctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc
    24001 ttgtcgggat aatgtcagtg ggagctctgg tagttgcgtc atggctggtg ccagataaga
    24061 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    24121 gtgtcgtcgc agttttaagg cttctcggac gaaccgagca tgctcaccaa ccgcggagcg
    24181 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    24241 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    24301 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    24361 agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtctttgc tttttcttgc
    24421 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    24481 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    24541 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    24601 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    24661 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    24721 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    24781 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    24841 acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga tgatatgtgg
    24901 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    24961 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    25021 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    25081 cgatcgggct gatgcactgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    25141 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    25201 catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    25261 agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    25321 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    25381 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    25441 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    25501 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    25561 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    25621 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg
    25681 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac
    25741 tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg
    25801 ctgatgaaat cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca
    25861 ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat
    25921 cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacatacac
    25981 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc
    26041 acgaatttaa ccgccacggt gcgcgctatc cttcctataa caccattgta ggcagcggtg
    26101 aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac ggtgacctgg
    26161 tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc
    26221 cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg ctggagtctc
    26281 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag
    26341 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt aatgttgatg
    26401 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    26461 gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    26521 ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac
    26581 aatatcgcgg tatcggcatt cgtattgaag acgacattgt gattaccgaa accggcaacg
    26641 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggctgctg
    26701 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    26761 ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag cgactgcgcc
    26821 agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg cgggtacctg
    26881 tcagcaactg gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac
    26941 taccgtgcat gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta
    27001 ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc
    27061 attgctgcga aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc
    27121 ccgtactcag agtcatgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt
    27181 gctgatagcc gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca
    27241 gcaggagcct tacgaacaac tggcagtgat tgccaacgtt gctacttctg ttgcgcacga
    27301 aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    27361 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    27421 gagcgacgag aagttttgcc gtgaactcca gtctgccttt ggctggcgac tggggcaaat
    27481 tacccacgcc ggtaaacgca gtgcttatcc gctggcatta acccgcgccg ccagaccgat
    27541 tacccatcgc actgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca
    27601 agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca
    27661 tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca
    27721 gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg
    27781 ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc
    27841 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    27901 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    27961 cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac
    28021 cggaacctct ggcggtagat gcactaccac aactgcgcgt ttcggctatc aatgccgcca
    28081 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctcgctcgt agggccagtt
    28141 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatctcg tttgacgatc
    28201 aaagcatggg ctatagccat cttgggcaca tcgttgaaaa ctcagtgatt cactacgcgc
    28261 tgtggaacaa agcgcagcag tcgtcagata ttactctgtt ggcccccaca gaattacagc
    28321 aggtcgcctg gggagaaaat gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    28381 cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc
    28441 cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac
    28501 cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc
    28561 ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa gcgcagcgga
    28621 tgcagcaggc aagtgaagac gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    28681 ttggcttatg caaggttgag agcgcgcgtc aggtgttccc gctgacgggg cgttatgcgc
    28741 gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    28801 tggcggggca gggcgttaat ctcggattta tggacgccgc agagctgatt gctgaactga
    28861 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgc cgctatgaac
    28921 gtagccgcaa gcacagcgcg gcgctgatgc tggctggtat gcagggtttt cgcgatctct
    28981 tttccggcgc taatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca
    29041 cgcttcctgg cgttaagccg caacttatcc gccaggcaat gggattaaac gatttgcctg
    29101 aatggctgcg ttaaaaattt ctcctctgtt gtttatttga tacccatcac actttcatct
    29161 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    29221 cgcattatat tttctaatac catgattttt tgatttagtg ttttttgaca tttttttagc
    29281 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaattgg
    29341 tgtatttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    29401 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    29461 aacgattgcg ttggggaccg ggagtggctc cgatgctggg ttttgtggtg ataatttcac
    29521 catgaaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    29581 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    29641 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    29701 ttgatgtgtc gcatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    29761 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc ctttactcgg
    29821 ggatgttgaa tgcctctggc ggtgtgatag atgatctcat cgtctactac tttactgaag
    29881 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    29941 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    30001 tgcaagggcc gaatgctcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg
    30061 cggtggaagg gatgaaaccg ttctttggcg tgcaggtggg cgatctgttt attgccacca
    30121 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    30181 tctggcgtgc gctggtggaa gcgggcgtta agccctgcgg tctgggggcg cgtgacacgc
    30241 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    30301 tagccgccaa catgggctgg actatcgcct gggaaccgac agatcgtgac tttatcggtc
    30361 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    30421 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    30481 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    30541 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    30601 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    30661 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    30721 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    30781 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    30841 gggcgatgac tgcgcggttg ccgaatcggt aaaagcggcg tcagacattt atgcgccagt
    30901 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    30961 cgaaccgtat gcaggcggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    31021 atcactgctg gatgcgaccg catacgaagc attgttagaa gatgagtaac ggctttattc
    31081 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    31141 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    31201 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    31261 aacagcaaga aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga
    31321 ttgtgccgaa agatattcag cttgcgacac caccgcaggt tggcgcaccg gcgaccgaat
    31381 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    31441 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    31501 atccgggctg gtataccgcg tacactccgt atcagcctga agtctcccag ggccgccttg
    31561 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    31621 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    31681 gcaaactgaa aaatgccaac cgcttcttcg tggcttccaa tgtgcatccg caaacgctgg
    31741 atgtggtccg tactcgtgct gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    31801 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    31861 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    31921 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    31981 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    32041 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    32101 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    32161 ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    32221 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    32281 accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg
    32341 cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg
    32401 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg atcacccttg
    32461 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag tgtgctgctg ggcgataacc
    32521 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc
    32581 ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc tatcacagcg
    32641 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    32701 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    32761 tcacttggac ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    32821 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    32881 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    32941 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    33001 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    33061 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    33121 acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc
    33181 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    33241 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    33301 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    33361 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    33421 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    33481 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    33541 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    33601 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    33661 aaaccggcat cagcgagctg gacattgcca agcgcctgat cgactacggt ttccacgcgc
    33721 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    33781 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    33841 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    33901 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    33961 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    34021 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgagtattt
    34081 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    34141 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    34201 atttaccctc accccgaccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg
    34261 aagattgagc cagtttgtta gcaatctcaa agatacgtca acgaattaat ttttctcgga
    34321 aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc
    34381 attactgttg gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc
    34441 ggcgcaggaa gtgatgaact taataatgca agccggtggc aaagcattcg tgctccaggc
    34501 ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga
    34561 accgctagca gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa
    34621 ccttaccgca gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg
    34681 ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt
    34741 caatgtctct tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc
    34801 atcgaaaggg gcgattgatt cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg
    34861 gatccgcgtt aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcggcgg
    34921 cgagcctgga cgcgtcgatc gcgttaagtc gaacatcccc atgcagcgtg gtggacaggc
    34981 agaagaggtc gcgcaggcca ttgtctggct actaagtgat aaagcctctt acgtcacggg
    35041 aagttttatc gatttggcgg gcgggaaata aaacagggaa gttgtctgac cggatgcaac
    35101 aagtattgca tccggtactt catcgactta aagcttctcg ccgttgctgg caatcacctc
    35161 tttgtaccag ttaaagctct tcttacgtga acgcgacata tcgccagtac cgtcgtcatg
    35221 tttattcaca tagataaagc cgtagcgttt gctgtactgc ccggtagtga atgacacgca
    35281 gtcgatgcag ccccacggtg tgtagcccat cagatccaca ccatcgtaag tcactgcttt
    35341 tttcatctct tcaatatggg cgcgcaggta gtcaatgcgg tagtcgtcgt tgatgctgcc
    35401 atcttcttcc actttgtcgt aagcgccaaa accgttttcg acaataaaca gcggcctctg
    35461 ataacgctca tacagttcgc aaagggcata gcgcagacct actggatcaa tctgccagcc
    35521 ccagtcagac gctttaacat acgggttcgg tacgctgcct tcaaaaccag agatcgcatc
    35581 gccggtgccg ccttcggcct tcactgcgtt ggtcatgtaa tagctgaaac caagataatc
    35641 gcaggtgcct tcgcgcagta catcgagatc gccgtcttcc attttgatat taaatccgcg
    35701 acgttcccac tcgttcaata cataggacgg gtaatagccg catagctgca catcggtaaa
    35761 gacgtagcgt tcgcgcatcg actcctgagc gaacatcaca tcgtccgggt tacatgagta
    35821 gggatagagc ggcaccatcg ccagcataca gccgactttc atctccgggt taatgcgacg
    35881 cgcggctttc actgccaggg cgctggcgac aaactggtga tgcagcacct ggtacatcgt
    35941 ctcttccggg ttgtcatgct cggtatacac cacgccggag cagcagtaac caaacagcgg
    36001 cgcacgccag ttacgctggt tgtttatttc gttgaaggtc atccagtatt tgactttatg
    36061 cttgtagcgt tcgaatacca cttccgcgaa acggacaaag aaatcaacga atttacggtt
    36121 ggtccagcta ccgtattgct gcaccagatg cagcggcatt tcaaagtggg agagggttat
    36181 caccggttcg atgttgtatt tcagcagttc atcgaacaga gagtcgtaaa acttcagccc
    36241 ttcttcgttt ggctgagctt catcaccttt tggaaaaata cgcgtccagg cgatggaagt
    36301 ccgaaaacat ttgaagccca tttcggcaaa tagcttgatg tcttccttgt agtgaccata
    36361 aaaatcaacg gcttcatggt ttggatagta ttttcctggc aagacttctt tggtgatttc
    36421 gcgcggcacg ccgtgtgcgc cacaggtcag aacgtcacaa atgctcggtc ctttgccgcc
    36481 tttgttccag ccgccttcga cctgatgagc ggcaacggcg ccaccccata agaaatcttt
    36541 cggtaaggtg agttttttca ctatcatagg ctccagatca gtcaattctt acaggaaata
    36601 gtaccatgca gccaaacgac atcacttttt tcaacgtttc caggatgaca ttctggctgg
    36661 gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg gtgatgtgct
    36721 tcgtgtcgga cgttttgaag atgacggtta tttttgcacg attgaagtca ccgcaacctc
    36781 aaccgtaacg ctggatacac tgacggaaaa acatgcagag caggaaaata tgaccctgcc
    36841 tgaactgaaa aaggtcattg ctgacatcta tcccggtcag acacaatttt atatgattga
    36901 atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttcgcat ttaatttcat
    36961 gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg ttcactggaa
    37021 ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctctg gagtaagtta
    37081 tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt gccaacagcg
    37141 tcagtcacgg cattgggctg gtgtttggta tcgttgggct ggtgttgcta ctggttcagg
    37201 cggtggatct caatgccagc gccacagcga taaccagtta cagcctctat ggcggcagta
    37261 tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa cgggcaaaaa
    37321 tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga acctacacgc
    37381 cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt gttatctgga
    37441 gcctagcatt gctggggatt ctgttcaaac tgaccatcgc gcaccgattc aaaattttat
    37501 ctctggtgac ttatctggcg atgggctggc tgtcgctggt ggtaatttat gaaatggcag
    37561 ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggcgtggtt tattcgctcg
    37621 gggtgatttt ctacgtctgc aaacgcattc catacaacca tgccatctgg cacggcttcg
    37681 tgctcggcgg tagtgtgtgc cactttctgg cgatctattt gtatattggg caggcgtaat
    37741 gggtatgcct ttgtaggcct gataagacgc gtcagcgtcg catcaggcat cggagcgcaa
    37801 ccgccggatg cggcgtgaac gccttatccg atctacgggc ctcgtccctg ttgtgataaa
    37861 ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat aaatgcctga
    37921 tgcgatacag agatttactc ttcgagcgaa tacggcagcg gctcgatatg cagtgtattc
    37981 gcatcgtcgc gtacgcggaa gatgctgtcc ggttccatat cgttattcat gacgacctgt
    38041 accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt acgacgccag
    38101 ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct ggcactacct
    38161 gccagcaacc agagcgcgcg tttgttggca ccacggaatt ttgctcgcgc caccatctct
    38221 tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag attggtcgcc
    38281 tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaaccagc ttcaatgttt
    38341 aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc
    38401 atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat
    38461 agcaaagtgg tcgcgccttc tttgactacc tgtttttctt tcgaaggcag ttcgctaaag
    38521 agatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg
    38581 tctggcgcga tggtcacttt agagaatacc gcatattttt tcagttcagt cagctgcggt
    38641 tcacgcacgc tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa cagacgtaaa
    38701 ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg atcttctgcc
    38761 atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta
    38821 atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc
    38881 gtaggctgac gtggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg
    38941 cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc actacgcgat
    39001 ctgtgccttc tgcgaggcga ctgccaaaaa aatgaagccc ttgaattgtt gtgaattaat
    39061 ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aagtcgaaaa acacgttaca
    39121 atagccgggt actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg
    39181 aattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt
    39241 cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga
    39301 atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga
    39361 actggaaata atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat
    39421 ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg
    39481 ccgctgttat tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttac
    39541 tttcgctggt ggtatttgat tgcgttcgca gccagcggtg tattaatgct cgccaggggg
    39601 aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca
    39661 aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat ggcggtaaac
    39721 ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg gatttacggc
    39781 ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata tgcgctaagc
    39841 cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc
    39901 tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac
    39961 agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag
    40021 tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg
    40081 aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaat aattttacct
    40141 tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcacca
    40201 aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag
    40261 tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct
    40321 gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa
    40381 gcttctcgat tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt agtcacggca
    40441 caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa caggagctgg
    40501 cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt
    40561 tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga
    40621 cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg
    40681 ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc
    40741 tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc
    40801 cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcgccat
    40861 taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg
    40921 ggctgcgtgt ctctgaactg gtcgggctga caatgagtga tatcagcctg cgtcagggcg
    40981 tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt gccgttaggg gaagaggcgg
    41041 tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat ggtgtgtcaa
    41101 ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc
    41161 gcattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt
    41221 tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc gtggtgcaga
    41281 tgctactggg ccacagcgat ctttccacca cgcaaattta tacgcatgtc gctaccgagc
    41341 gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa
    41401 gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggctttgctc
    41461 aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata
    41521 ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggt gtgttgtaca
    41581 tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagt ggcacggctc
    41641 cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga
    41701 tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg
    41761 gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg atcaccgtgc
    41821 gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa atgaaagcta
    41881 tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg
    41941 caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg
    42001 ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc
    42061 cgaaagacat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg
    42121 cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc
    42181 tgaattgcct cccttgctgc gccgtttata cgccagccgg ggagtacgca gtgcgcaaga
    42241 actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctaagcggtg tcgaaaaggc
    42301 cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg tcggtgattt
    42361 cgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg
    42421 cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga
    42481 agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat
    42541 ttcctcccat gcgggagttg agcacgctcg ctcgttgggc atcccggtta ttgttaccga
    42601 tcaccatttg ccgggcgaca cattacccgc agcggaagcg atcattaacc ctaacttgcg
    42661 cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct
    42721 ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgcggca tcgcaattcc
    42781 taacctggca gaactgctgg atctggtagc actggggaca gtagcggacg tcgtgccgct
    42841 ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg ccggaaagtg
    42901 ccgtccaggg attaaagcgc tgctggaagt ggcaaatcgc gatgcacaaa aactagccgc
    42961 cagcgattta ggttttgcgc tgggaccgcg tctcaatgct gccgggcgac tggatgatat
    43021 gtccgttggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa
    43081 tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc aggttgaagc
    43141 cctgaccctg tgcgagaaac tggagcgcag ccgtgacacg ctacccggcg ggctggcaat
    43201 gtatcacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgta tcaaagagcg
    43261 ttttcaccgt ccggttatcg cctttgcgcc agcaggtgac ggtacgctga aaggttccgg
    43321 tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc
    43381 cggcatgatg ctcaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga
    43441 taaattcgaa ctctttcaac aacggtttgg cgaactggtt actgagtggc tggccccttc
    43501 gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgaccatgga
    43561 agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt
    43621 tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaggtgat
    43681 ggtcgaaccg gtcggcggcg gtccactgct ggatggtatt gcttttaatg tcgataccgc
    43741 tctctggccg gataacggcg tgcgcgaagt gcaactggct tataagctcg atatcaacga
    43801 gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca
    43861 tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga
    43921 ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca
    43981 ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag
    44041 agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac
    44101 gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc
    44161 aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg
    44221 acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc
    44281 agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgt tacctcgata
    44341 ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt
    44401 atctgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg
    44461 aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc
    44521 tgcgtacaga aactggcgtt caccgcctgg tgcgtaaaag cccgtttgac tccggcggtc
    44581 gtcgccacac gtcgttcagc tctgcgtttg tttatccgga agttgatgat gatattgata
    44641 tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc gcgggcggtc
    44701 agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccgacc gggatcgtga
    44761 cccagtgcca gaacgaccgt tcccagcaca agaacaaaga tcaggccatg aagcagatga
    44821 aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag gcgatggaag
    44881 ataacaaatc tgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc
    44941 gcattaaaga tctgcgcacc ggggtagaaa cccgcaacac gcaggccgtg ctggacggca
    45001 gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg
    45061 aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc
    45121 gtgagaagct ggcgaacctg cgtgagcagg gaattgcctt cccgaacgat ttccgtcgcg
    45181 atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag
    45241 cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag
    45301 cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt gcccgtgacg
    45361 atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc gacatcctcg
    45421 gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc
    45481 tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc
    45541 aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgtaaca
    45601 cctttaaagt acgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct
    45661 ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct gcgcgtccgt
    45721 ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt
    45781 acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc
    45841 gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa ctctacatgg
    45901 cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac
    45961 aggatattct cggtaagacg gaagtgacct acggcgacgt gacgctggac ttcggtaaac
    46021 cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg
    46081 cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt atccacgttg
    46141 agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac
    46201 atctgattca gccgaccttc attactgaat atccggcaga agtttctccg ctggcgcgtc
    46261 gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt ggtcgtgaaa
    46321 tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc ttcctggatc
    46381 aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa gattacgtta
    46441 ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc gaccgtatgg
    46501 taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc
    46561 cggtgaaata agcattacgt tatgctcaca accccggcaa atgtcggggt tttttattta
    46621 agctgggtaa atgcagataa tcgtattctg gcttcgcgat ttgtcgcctg catcaccatc
    46681 cacggactga acgcccacgg cgtggcatca ataccgcgta atacatcagc taaatcacac
    46741 cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca
    46801 aataccggac acacttcatt ttccacaatg cctctcggat cggtggcgcg gtagcgaaag
    46861 tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg
    46921 atcaccgcgt cttcgctgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac
    46981 acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg ccccttggca
    47041 ttaaacagcc aactggagaa cgcgagatgt aagagggtgt ctgccgtgtg tgcggcatac
    47101 ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc
    47161 ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga
    47221 ttaaccaaga gagcgtttat cgacaaccgc ccgttttgag cggatgccga caggtaaaaa
    47281 ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt
    47341 cagaaatgac aggaatagag agagtaaaaa gcaacaacag actgaatata gcgcgcatta
    47401 aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata
    47461 cgtactgtcc tgacttctaa atctttatca gagacggatt ccttcaccag gtcagcatga
    47521 tgctgatagc cattaaagaa gacgttcagc acaacggcgc taagggttgc gagcataatg
    47581 ccgctatgta gcagcggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc
    47641 gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg
    47701 gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcact
    47761 agaccagcgc cgcccagcac aaattgcgga atggaggcta ccagcaccgc catttttggc
    47821 accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg
    47881 cgcatcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa gctgttaaac
    47941 gtgccgccta tcattgtccc tacgccatcg acacgcagcc cgcaaataat atcgtgtgaa
    48001 gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac
    48061 acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat cggcatacca
    48121 aacgacatcg gggtgacaat cgcaaaccac gaggcatcat gtagcccgga taaattgact
    48181 tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg
    48241 gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag
    48301 atacctaaat aaacgggatt accatattgc ggattccctt tacctccagc ggcccagtca
    48361 atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt aaccagtggc
    48421 gggaataaag gcatcaagcg accaataagt ggcgctaata atgtggtgat aaaacccgcg
    48481 gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc
    48541 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    48601 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    48661 atgagcatcg caatagcatc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    48721 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    48781 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt
    48841 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagaataa aaagcaaccg
    48901 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    48961 ggagttagag acaggaattg cggaggcaat cacataatcg tgcttaaacc acgaaaatga
    49021 caagagaaac tgacaatgga attatctgta attttctcat attgatattt cattaatcat
    49081 tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc ttttgatcgg
    49141 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    49201 aa
//
LOCUS       DAJTNP010000021        48201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTNP010000021 DAJTNP010000000
VERSION     DAJTNP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            57..350
                     /locus_tag="OO160_001313"
     CDS             57..350
                     /locus_tag="OO160_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5523502.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(391..1581)
                     /gene="nepI"
                     /locus_tag="OO160_001314"
     CDS             complement(391..1581)
                     /gene="nepI"
                     /locus_tag="OO160_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5523503.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1792..2244)
                     /locus_tag="OO160_001315"
     CDS             complement(1792..2244)
                     /locus_tag="OO160_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5523504.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2297..3631)
                     /gene="adeQ"
                     /locus_tag="OO160_001316"
                     /gene_synonym="yicO"
     CDS             complement(2297..3631)
                     /gene="adeQ"
                     /locus_tag="OO160_001316"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5523505.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3806..5572
                     /gene="adeD"
                     /locus_tag="OO160_001317"
     CDS             3806..5572
                     /gene="adeD"
                     /locus_tag="OO160_001317"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5523506.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5618..7009)
                     /gene="uhpT"
                     /locus_tag="OO160_001318"
     CDS             complement(5618..7009)
                     /gene="uhpT"
                     /locus_tag="OO160_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5523507.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7147..8466)
                     /gene="uhpC"
                     /locus_tag="OO160_001319"
     CDS             complement(7147..8466)
                     /gene="uhpC"
                     /locus_tag="OO160_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5523508.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8476..9978)
                     /gene="uhpB"
                     /locus_tag="OO160_001320"
     CDS             complement(8476..9978)
                     /gene="uhpB"
                     /locus_tag="OO160_001320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5523509.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9978..10568)
                     /gene="uhpA"
                     /locus_tag="OO160_001321"
     CDS             complement(9978..10568)
                     /gene="uhpA"
                     /locus_tag="OO160_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5523510.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10643..10933)
                     /gene="ilvN"
                     /locus_tag="OO160_001322"
     CDS             complement(10643..10933)
                     /gene="ilvN"
                     /locus_tag="OO160_001322"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5523511.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10937..12625)
                     /gene="ilvB"
                     /locus_tag="OO160_001323"
     CDS             complement(10937..12625)
                     /gene="ilvB"
                     /locus_tag="OO160_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5523512.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12731..12829)
                     /gene="ivbL"
                     /locus_tag="OO160_001324"
     CDS             complement(12731..12829)
                     /gene="ivbL"
                     /locus_tag="OO160_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5523513.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13394..13483
                     /gene="tisB"
                     /locus_tag="OO160_001325"
     CDS             13394..13483
                     /gene="tisB"
                     /locus_tag="OO160_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5523514.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13763..14947
                     /gene="emrD"
                     /locus_tag="OO160_001326"
     CDS             13763..14947
                     /gene="emrD"
                     /locus_tag="OO160_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5523515.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14955..15452)
                     /locus_tag="OO160_001327"
     CDS             complement(14955..15452)
                     /locus_tag="OO160_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5523516.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15449..15811)
                     /locus_tag="OO160_001328"
     CDS             complement(15449..15811)
                     /locus_tag="OO160_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5523517.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15801..16148)
                     /locus_tag="OO160_001329"
     CDS             complement(15801..16148)
                     /locus_tag="OO160_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5523518.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16257..16670
                     /locus_tag="OO160_001330"
     CDS             16257..16670
                     /locus_tag="OO160_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523519.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16717..18159)
                     /locus_tag="OO160_001331"
                     /pseudo
     CDS             complement(16717..18159)
                     /locus_tag="OO160_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18194..19909)
                     /locus_tag="OO160_001332"
     CDS             complement(18194..19909)
                     /locus_tag="OO160_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5523520.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVIITFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20076..20969
                     /locus_tag="OO160_001333"
     CDS             20076..20969
                     /locus_tag="OO160_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5523521.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20966..21780)
                     /locus_tag="OO160_001334"
                     /pseudo
     CDS             complement(20966..21780)
                     /locus_tag="OO160_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21780..23396)
                     /locus_tag="OO160_001335"
     CDS             complement(21780..23396)
                     /locus_tag="OO160_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5523522.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23691..24365
                     /locus_tag="OO160_001336"
     CDS             23691..24365
                     /locus_tag="OO160_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5523523.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24404..26065)
                     /locus_tag="OO160_001337"
     CDS             complement(24404..26065)
                     /locus_tag="OO160_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5523524.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26261..26689)
                     /gene="ibpB"
                     /locus_tag="OO160_001338"
     CDS             complement(26261..26689)
                     /gene="ibpB"
                     /locus_tag="OO160_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5523525.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26801..27214)
                     /gene="ibpA"
                     /locus_tag="OO160_001339"
     CDS             complement(26801..27214)
                     /gene="ibpA"
                     /locus_tag="OO160_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5523526.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27208..27280)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27519..27851
                     /locus_tag="OO160_001340"
     CDS             27519..27851
                     /locus_tag="OO160_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5523527.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27853..29091)
                     /locus_tag="OO160_001341"
     CDS             complement(27853..29091)
                     /locus_tag="OO160_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5523528.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            29192..30292
                     /gene="cbrA"
                     /locus_tag="OO160_001342"
     CDS             29192..30292
                     /gene="cbrA"
                     /locus_tag="OO160_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5523529.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30253..31590)
                     /locus_tag="OO160_001343"
     CDS             complement(30253..31590)
                     /locus_tag="OO160_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5523530.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31665..32813)
                     /gene="dgoD"
                     /locus_tag="OO160_001344"
                     /pseudo
     CDS             complement(31665..32813)
                     /gene="dgoD"
                     /locus_tag="OO160_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32810..33427)
                     /gene="dgoA"
                     /locus_tag="OO160_001345"
     CDS             complement(32810..33427)
                     /gene="dgoA"
                     /locus_tag="OO160_001345"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5523531.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33411..34289)
                     /gene="dgoK"
                     /locus_tag="OO160_001346"
     CDS             complement(33411..34289)
                     /gene="dgoK"
                     /locus_tag="OO160_001346"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5523532.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34286..34975)
                     /gene="dgoR"
                     /locus_tag="OO160_001347"
     CDS             complement(34286..34975)
                     /gene="dgoR"
                     /locus_tag="OO160_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5523533.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35253..35909
                     /locus_tag="OO160_001348"
     CDS             35253..35909
                     /locus_tag="OO160_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523534.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35955..36767)
                     /gene="yidA"
                     /locus_tag="OO160_001349"
     CDS             complement(35955..36767)
                     /gene="yidA"
                     /locus_tag="OO160_001349"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5523535.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36881..37279)
                     /locus_tag="OO160_001350"
     CDS             complement(36881..37279)
                     /locus_tag="OO160_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5523536.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37519..39933)
                     /gene="gyrB"
                     /locus_tag="OO160_001351"
     CDS             complement(37519..39933)
                     /gene="gyrB"
                     /locus_tag="OO160_001351"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5523537.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39962..41035)
                     /gene="recF"
                     /locus_tag="OO160_001352"
     CDS             complement(39962..41035)
                     /gene="recF"
                     /locus_tag="OO160_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5523538.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41035..42135)
                     /gene="dnaN"
                     /locus_tag="OO160_001353"
     CDS             complement(41035..42135)
                     /gene="dnaN"
                     /locus_tag="OO160_001353"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5523539.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42140..43543)
                     /gene="dnaA"
                     /locus_tag="OO160_001354"
     CDS             complement(42140..43543)
                     /gene="dnaA"
                     /locus_tag="OO160_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5523540.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43840..43920
                     /locus_tag="OO160_001355"
     CDS             43840..43920
                     /locus_tag="OO160_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523541.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44150..44290
                     /gene="rpmH"
                     /locus_tag="OO160_001356"
     CDS             44150..44290
                     /gene="rpmH"
                     /locus_tag="OO160_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5523542.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44307..44666
                     /gene="rnpA"
                     /locus_tag="OO160_001357"
     CDS             44307..44666
                     /gene="rnpA"
                     /locus_tag="OO160_001357"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5523543.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44630..44887
                     /gene="yidD"
                     /locus_tag="OO160_001358"
     CDS             44630..44887
                     /gene="yidD"
                     /locus_tag="OO160_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5523544.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44890..46536
                     /gene="yidC"
                     /locus_tag="OO160_001359"
     CDS             44890..46536
                     /gene="yidC"
                     /locus_tag="OO160_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5523545.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46642..48006
                     /gene="mnmE"
                     /locus_tag="OO160_001360"
     CDS             46642..48006
                     /gene="mnmE"
                     /locus_tag="OO160_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5523546.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11941 a        13039 c        12187 g        11034 t
ORIGIN      
        1 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
       61 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
      121 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
      181 gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
      241 cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc
      301 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
      361 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
      421 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
      481 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
      541 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
      601 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
      661 aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg
      721 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc
      781 aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc
      841 cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga
      901 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
      961 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     1021 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     1081 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     1141 cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat
     1201 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     1261 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     1321 aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc
     1381 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     1441 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     1501 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     1561 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     1621 ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg tgttgcttga
     1681 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     1741 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     1801 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     1861 atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga cctgagcatc
     1921 ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca gcaggttctg
     1981 ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg cgttttgcag
     2041 gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat
     2101 ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg
     2161 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     2221 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     2281 aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga gctgcgacca
     2341 ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg
     2401 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     2461 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     2521 tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga accatcgcca
     2581 ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca gccgttagcc
     2641 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     2701 taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     2761 tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca attaatgttc
     2821 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     2881 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     2941 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     3001 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     3061 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     3121 tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat ccgattccac
     3181 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     3241 acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
     3301 aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
     3361 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     3421 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     3481 ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     3541 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     3601 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     3661 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     3721 acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     3781 ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
     3841 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     3901 tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt ccggcccaat
     3961 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata ctccggcttt
     4021 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     4081 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     4141 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     4201 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     4261 cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     4321 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     4381 aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc tgacgctgga
     4441 cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg cgggtattga
     4501 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     4561 gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac cgttgatcaa
     4621 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     4681 ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     4741 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
     4801 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     4861 cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct tacaggcgga
     4921 agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg cccgccagcc
     4981 agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc gggtcattga
     5041 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     5101 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     5161 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg cgacagtcag
     5221 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     5281 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag
     5341 tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
     5401 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     5461 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     5521 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaaa
     5581 gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     5641 tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc atcaggcaga
     5701 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga
     5761 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     5821 ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     5881 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     5941 gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc gtggcgataa
     6001 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     6061 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     6121 ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac
     6181 caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
     6241 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
     6301 cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg
     6361 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
     6421 agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac
     6481 ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc
     6541 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     6601 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     6661 ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg aacggcagga
     6721 attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc acgccataag
     6781 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
     6841 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
     6901 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     6961 gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg ggttactcct
     7021 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
     7081 agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
     7141 gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
     7201 aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
     7261 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
     7321 cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac caattaacat
     7381 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
     7441 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa
     7501 attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc
     7561 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
     7621 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata
     7681 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt
     7741 gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc
     7801 gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca
     7861 gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca
     7921 gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg
     7981 cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa
     8041 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
     8101 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat
     8161 aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa
     8221 cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
     8281 gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc gcgtgaagta
     8341 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
     8401 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
     8461 caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg
     8521 cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
     8581 gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag
     8641 catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt
     8701 cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
     8761 taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc
     8821 catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg
     8881 acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat
     8941 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa caatgcccgc
     9001 ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac gcgccacgtc
     9061 gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag
     9121 ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc ccagtaacaa
     9181 ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat cgcgccaggt
     9241 ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccagcc
     9301 atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa agcgcgacag
     9361 ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca agtaccagac
     9421 cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga gcggcagcca
     9481 ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg tcagaccgcc
     9541 agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
     9601 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
     9661 ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac taccgatcat
     9721 caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
     9781 cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag
     9841 accaaacgga aataacagca ccgccatatc agggcgctca accagatgca ggctgatact
     9901 ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
     9961 ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc tacgtcgtta
    10021 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    10081 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg
    10141 cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc
    10201 gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc atccgggcta
    10261 cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga
    10321 ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct tagcagctcc
    10381 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    10441 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc
    10501 agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    10561 gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg
    10621 atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg
    10681 gatcggactg attacgctgc actttcacga catcttccag cttagcgatt tggcttatca
    10741 tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct
    10801 gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa
    10861 cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg ttgttatgag
    10921 ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca
    10981 tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat
    11041 tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga
    11101 ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg gcggcaaaaa
    11161 cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca
    11221 tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca
    11281 tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg
    11341 cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact
    11401 ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg
    11461 tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc
    11521 ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca
    11581 actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt
    11641 catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat
    11701 cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc
    11761 ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa atatagttgg
    11821 tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcagca
    11881 tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca
    11941 cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt ttggcagcgt
    12001 taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca acggcttttt
    12061 ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt
    12121 ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct
    12181 gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc
    12241 cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccag
    12301 tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc accaggttag
    12361 tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccattc
    12421 cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg atttgcgtgc
    12481 tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct
    12541 taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg
    12601 tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg
    12661 aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt
    12721 gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg
    12781 cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg
    12841 ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa
    12901 tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg cagataacaa
    12961 aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact
    13021 gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa
    13081 ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg tccaaatatt
    13141 actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt tatgcggggg
    13201 ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta
    13261 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
    13321 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
    13381 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
    13441 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
    13501 ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagtcggatg
    13561 cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat tgccgctttt
    13621 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
    13681 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
    13741 ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt
    13801 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
    13861 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
    13921 gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg tcggtcgccg
    13981 accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
    14041 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
    14101 cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt tgcgccatgc
    14161 taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg
    14221 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
    14281 tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg atgcaccgcg
    14341 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
    14401 gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct ccggcgtgct
    14461 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
    14521 tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct tctcaacgtt
    14581 gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga tccccgactg
    14641 gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc
    14701 cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct tcctggcggg
    14761 cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
    14821 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga ccttaatggg
    14881 attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc
    14941 cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
    15001 atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc
    15061 gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg
    15121 cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg
    15181 gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg
    15241 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
    15301 tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg
    15361 tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc
    15421 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
    15481 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
    15541 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
    15601 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
    15661 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
    15721 agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg
    15781 caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccgcc
    15841 acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc
    15901 agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg
    15961 gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc
    16021 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc
    16081 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa
    16141 attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa
    16201 gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg
    16261 gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa
    16321 tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa
    16381 gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg
    16441 aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa
    16501 gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg
    16561 aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt
    16621 tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa
    16681 taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc
    16741 caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc
    16801 atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg
    16861 acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    16921 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    16981 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag
    17041 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    17101 aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt
    17161 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    17221 atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    17281 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    17341 gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg
    17401 cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata
    17461 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    17521 gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta
    17581 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    17641 atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac
    17701 cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa
    17761 atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    17821 aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt
    17881 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    17941 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    18001 cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac
    18061 cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    18121 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    18181 caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    18241 gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac
    18301 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc
    18361 caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt
    18421 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    18481 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    18541 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    18601 ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg
    18661 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttcg
    18721 cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    18781 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    18841 tgaataatgt actggcgcta ttcagaaagc cgttgaaggt aatgatcacc gcaccaaata
    18901 acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc agcgtcgggt
    18961 aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca
    19021 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    19081 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    19141 aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    19201 caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa
    19261 agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    19321 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    19381 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    19441 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    19501 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    19561 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    19621 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    19681 ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg
    19741 aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    19801 gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg
    19861 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    19921 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    19981 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    20041 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    20101 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    20161 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    20221 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    20281 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta
    20341 ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    20401 agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt cagcaaagcg
    20461 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    20521 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    20581 ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    20641 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    20701 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    20761 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    20821 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    20881 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    20941 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    21001 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    21061 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    21121 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    21181 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    21241 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    21301 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta
    21361 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    21421 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    21481 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    21541 aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    21601 cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc
    21661 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    21721 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    21781 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    21841 agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    21901 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    21961 tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc
    22021 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg
    22081 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    22141 tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac
    22201 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    22261 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    22321 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    22381 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc
    22441 atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg
    22501 gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc
    22561 agggcaaaac cgccttccag gaacaacgat ttcagtggct cggcgctcag actgaactct
    22621 tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata
    22681 aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg
    22741 taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc
    22801 atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat caccaggaag
    22861 gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta
    22921 tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg
    22981 ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc caccgcgtcc
    23041 tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag
    23101 ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca
    23161 aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg
    23221 ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc
    23281 ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc ggcgaaggga
    23341 aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact gagcatttcc
    23401 acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg cagacctgga
    23461 gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc gtgatcgctt
    23521 tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga catctgtgag
    23581 cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat cgattaagca
    23641 gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag atgatctaca
    23701 aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg ctaaacagcc
    23761 ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg actatccgta
    23821 aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc agcggcactt
    23881 acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg gtggaggtgt
    23941 taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc
    24001 ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc
    24061 gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg ccggtaaaac
    24121 tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag
    24181 agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc gccgatagac
    24241 tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt
    24301 catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat
    24361 actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg atactccaga
    24421 agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag
    24481 tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca aacgccagcg
    24541 ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg
    24601 ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat
    24661 aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt
    24721 tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac
    24781 ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca
    24841 taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct ggcacaaaga
    24901 cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa
    24961 cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg
    25021 gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca
    25081 gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca
    25141 ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca
    25201 aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc agttgcgcat
    25261 tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg ataatggtat
    25321 ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata attttgtcgc
    25381 cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa
    25441 tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt
    25501 ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc
    25561 aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg
    25621 gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta ttggttaccg
    25681 caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg
    25741 cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg
    25801 agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca
    25861 ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct
    25921 gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta
    25981 tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac
    26041 tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta
    26101 cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag
    26161 aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa
    26221 aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac
    26281 gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa
    26341 tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca gccagcgtaa
    26401 aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg
    26461 gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc tctaaatctt
    26521 cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa
    26581 tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg gccagtttgt
    26641 caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc
    26701 cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg
    26761 agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt tcgatacggc
    26821 gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca
    26881 ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt
    26941 cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca
    27001 ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag
    27061 cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc
    27121 cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag
    27181 aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag
    27241 aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct
    27301 catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat cagcggactt
    27361 ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc
    27421 gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact
    27481 catcaggcca acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag
    27541 cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata
    27601 ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg attggcgacg
    27661 atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtaa
    27721 tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcgcgcg
    27781 ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc
    27841 ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc
    27901 cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt
    27961 ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc gattatccag
    28021 cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt
    28081 gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca gttgcacaat
    28141 gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca
    28201 gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg ttaaacgtcg
    28261 ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta acggcgcatc
    28321 acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata gctccggcac
    28381 tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca
    28441 tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt
    28501 actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct tttgtacgtt
    28561 gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg gatcgagttg
    28621 atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc taaagacatg
    28681 cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc
    28741 gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga agtcgtaatg
    28801 ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg acttcggatg
    28861 aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc
    28921 tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac
    28981 gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat tggtattggt
    29041 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
    29101 ctgatacaaa acggttcccg ccatcatact tcacaaggac ttcactttca gacaatagtc
    29161 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
    29221 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
    29281 gctggataaa aagcaccagt gcggtactga aggtttcagc aaaccttgcg gcggtctgct
    29341 ggcaccggac gcgcagcgat cttttattcg cgatggactg acgcttcctg tcgatgtgat
    29401 cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc gccgcatcgc tcacgcgtaa
    29461 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
    29521 gatccccgcc agcgttgagg tttatcacga tagcctgtgc cgaaaaatct ggcgtgagga
    29581 tgataaatgg catgtcattt ttcgtgcaga cggctgggag caacatatta ccgcccgcta
    29641 tctggtcggt gccgatggcg caaactcgat ggtgcggcga catctctacc cggatcatca
    29701 aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag aaacatccgg tgccgttcta
    29761 ctcctgcatc tttgataatg cgataactga ctgttactca tggagtatca gcaaagatgg
    29821 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgacgct
    29881 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gtgagaaatg
    29941 cacggtgttg tttccctcac gctggcagga ttttgtctgt ggtaaggaca acgcctttct
    30001 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
    30061 ggatagcgca gagattctgc gtgcggtgtt cctgaaacag ccggagaaga gcaacgccgc
    30121 ttactggcgc gccacacaca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    30181 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cgcatattcc
    30241 acagttgaaa gattatccaa cgcgcttcac atcacccacc agcaggatgt aagagagcgc
    30301 gccaatcaac gcgacggcgg agatataaac cagtgcaggc gcgaaaccgt aaccctgcgc
    30361 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    30421 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gagaccagcg accaggtaat
    30481 cgaagcaaaa ccgttgccga agaatgccag cgccatcagg cacataatca tcatcggatc
    30541 gttggtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    30601 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcga cccagccaga
    30661 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    30721 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaacacag
    30781 tgcagaagcc accgcaaatt gcccaagata aacgccgatc agtttacgat ggaacaccag
    30841 tttccagtct ttggctgtta acggctgacg cgcctctttc ttcaccggcg catcaccatc
    30901 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatac ctttggtcag
    30961 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    31021 gacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    31081 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    31141 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    31201 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cttggaataa
    31261 agtcgccact gaccaaccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    31321 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    31381 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    31441 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    31501 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    31561 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    31621 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    31681 ctgttatctt catgacgcca gagcggatta cgccagtccg gggcattttt actgaactca
    31741 ataactttag cttcgtcgat ttccacacct aagcccggtt tcgttaacgg tttaaagaag
    31801 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    31861 ttgtaatgaa tgcccatact ttgttactga agtaccgcgt tataggaaac aaagtcgata
    31921 tgcaggcaag ccgccagtgc aatcggtccg aacggacagt gcggcgcaag ggtcacatca
    31981 taggcttctg ccattccagc aattttgtag cattcggtaa taccgcctgc atgggagaga
    32041 tccggttgca gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    32101 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    32161 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    32221 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    32281 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    32341 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    32401 atttcgcgca gggttttaat gccgtcgata acatccgccg gacgatcgcc gccgacccaa
    32461 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac tggcgcattc
    32521 agcactttgc ctttgatatc ccataacgcc tggtcgatcc cggcgatggc gctcatcagg
    32581 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc gttgatgcgc
    32641 gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtacgg
    32701 gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc cttcatcggt ttcaattttc
    32761 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    32821 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    32881 cggcgcgata aagatcgctg cctaagcccg ccccaacaca acctgcgtct atccactgcg
    32941 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccggt ggtaataccg
    33001 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    33061 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcaaacgg
    33121 tcatgccgta gcccaccgcg cggcggatca cttcactatg gatattgggc gtaacgatga
    33181 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    33241 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctctgc tcccattgtg
    33301 gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    33361 ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat cagcgggagt ttagtttgcc
    33421 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgcc
    33481 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcagcga tgttccggcg
    33541 acaagggtga tggcgtgttg atgggtcaca tagtcgcgca tactggcaac ctctgcgcca
    33601 atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg gaagtgttcc cagcacatgc
    33661 gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg cattaaggcc gcgctcaagg
    33721 ccagccgcga aggcatcggc agagttttcc tgcggcggca aacctgcgcc aatcaatgag
    33781 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    33841 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca taacataaag agaggaagga
    33901 gccagagctc gcgcgccgat caattgtgtt tcttcgccgc gcatcacatt gtggttatcg
    33961 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    34021 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    34081 ttgctgccga ccattcctgc catcaccact ggcgtatttt cttcacgcca gtcggtcgtg
    34141 acttctgcta acactgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    34201 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    34261 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    34321 caatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    34381 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    34441 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    34501 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    34561 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    34621 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    34681 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    34741 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc cccgcgataa cgtttcattt
    34801 caatcagccg cttcgccatc agcgaacgga acacctcacg gatgatgttg cgcgaggttg
    34861 caaactcttc acagagctcc gcctcagccg gaagtggcga gccaggtacg tatttgccgt
    34921 gaacgatctg tttacccagc gtaatgacaa tgcgatcggt tttattgaga gtcatggaga
    34981 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cgcggcaact
    35041 ttgtagtacc agcgtgatga cgttcgcgtt tgccgcgcgc gtaatgtagt acaaacttat
    35101 attattgcgc tacaatttag atcaaaaaaa gaacaacaca taaaaaaatg acatgcgtcg
    35161 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    35221 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaattttaag ggatttttta
    35281 aggctgccgg tttattccca ctggcgctga tgctttcagg ctgtatctcg tatgctctgg
    35341 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcactg
    35401 ggctatcgca ggcaaaagat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    35461 tggattacct tatcactgat ggtgccgatg acatcgttaa gatgctcaat gatccagcac
    35521 ttaaccggca caatattcag gttgccgatg acgcaagatt tgttttaaat gcggggaaaa
    35581 agaaatttac cggcacaata tcgctttact actactggaa taacgaagaa gaaaaggcac
    35641 tggcaacgca ttatggtttt gcctgtggtg ttcaacactg taccaggtca ctggaaaacc
    35701 taaaaggcac aatccatgag aaaaataaaa acatggatta ctcaaaggtg atggcgttct
    35761 accatccatt taaagtgcga ttttatgaat actattcacc cagaggcatt ccggatggtg
    35821 tttccgcagc attactgcca gtgactgtta cactggacat cattactgca ccgctgcaat
    35881 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    35941 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    36001 aggttggatt ttgtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    36061 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    36121 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    36181 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    36241 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    36301 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    36361 ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc tgatatcgcg gttagcggtg
    36421 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    36481 aggaaacgat agtcgtcata gctgagagcg gtttgcgcca cggtgctacc atcagcggcc
    36541 ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    36601 tctttcaggt agttgtgcac acctgcatac ggacgacccg tcgttagcac gacattcacg
    36661 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    36721 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttcccaaggt
    36781 agattagttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    36841 gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag agtttccctt
    36901 tcagcagttc catgcctgcg gaaagcagat cgttattggc ttgtgctgac acttcacctt
    36961 gcggtgagag cgcatcaata atcttcggca attgctctgc cagtaaactg gaagccgtac
    37021 tggtatccac gccaagtttt tgcccgagat cggacaccgc atttgtgccg agcgccgact
    37081 ccagttgctc gccgctaact gattgatttc tctgttgatt actcaaccag gttgagagaa
    37141 tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgcca ccctgctcct
    37201 caacccaact taaaatcgcc tgatatttcc ccgcatcgcc tttcagaaag gcaccgacaa
    37261 cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac ccaaaaagta
    37321 tagatctgcg ggatgataat tgcggattgc agaaataaaa agggcggaga tgatctccgc
    37381 ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttacccggcc tacaaaatca
    37441 tgcaaaatca atatattgca ggagtctcgt aggactaata agcgtagcgc atcaggcacg
    37501 ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt tctcttcaat
    37561 aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaact ggtcagcggc
    37621 aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca tggtggtttc
    37681 ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg agaggccacg
    37741 acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg gctgacgacg
    37801 ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct cacccagcgt
    37861 gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat agtcagtatc
    37921 cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg cattggtgtg
    37981 gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc
    38041 gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca acgtcggctg
    38101 atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt tgatcatttt
    38161 ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca atgccggtgc
    38221 actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt actgatccat
    38281 cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt acagcggcgg
    38341 ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat agaagaaggt
    38401 caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga tgatgatgct
    38461 gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc caagcgcggt
    38521 gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt tctcgacgtt
    38581 aaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc
    38641 agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg gatcgcgttc
    38701 ctggcagtct gccagtttgc ccggcaggcc agctaagtcg agcgcacctt tacggcgggt
    38761 catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa ttttgccaac
    38821 cacgattttc gcatcagttg ggttttccag caggtattcc gccagcagtt cgttcatctg
    38881 ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct gggaggagaa
    38941 tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc
    39001 ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg cattcagggt
    39061 acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt
    39121 gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcagcg ccacttcaac
    39181 gccaataccg tctttttcgg tggagaagta gaagatattt gggtggatcg gcgttttgtt
    39241 cttgttcaga tattcaacga acgctttgat gccgccttca tagtggaagt ggtcttcttt
    39301 gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg acaactcacg
    39361 cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg tttcgaggct
    39421 gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg ccagcggagc
    39481 ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct gaataaccag
    39541 ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca gaccgccgga
    39601 cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca tgatcacttc
    39661 cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg
    39721 tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt gacccgcgag
    39781 cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac cggtgccgtc
    39841 atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc ctttcaggac
    39901 tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat ttatacttgg
    39961 gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg tccgacatgt
    40021 ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc gcttttaagc
    40081 ggctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca tctatcaggt
    40141 agagacaccg ccgcccgctt tcacgagtga ggaactctcc ttgcgccaga cgtaaggcgc
    40201 acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca ccgtcggcgc
    40261 gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg cgatcgcgtt
    40321 caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg cgctggaaag
    40381 agaaagtcag agaaaactca gggagaaatt gcttacaggt atcatccata tcggccgcga
    40441 taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg atcagttctt
    40501 tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca ttgcgctgct
    40561 tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc aggttcgttg tgaaagcatc
    40621 cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta aacccttctg
    40681 gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc gactttatgc ccgtccgtac
    40741 cgtcaatacg gactttgctg tcgccctgtt tgtctttggt taagccaatc gccgtctcgc
    40801 gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc ctgctcatgg cgaatgacgc
    40861 gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg gcttccagca
    40921 cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga gataaagcga
    40981 gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa gcgggtgagg gacattacag
    41041 tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa tctgcacgct
    41101 ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt tcagaacatc
    41161 cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg taacgtcgag
    41221 gatctcttcc gcttcttcct gttccgggtt gttggcagta attttcagct ggttttcgct
    41281 gacatacagg cgcacaccgc ggaatttctc gttagagaga atcgccgcgc gagcaaacgc
    41341 ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc gggttcttcg gcagaacgcg
    41401 acggtaatcc gggaagcgac catccaccag tttcgaggtg aaaataaagt cgccaacgtg
    41461 ggcacgaatg ttattgctgc caatctgcac acgcagcaga ttgtcgccgc cgtcgagcat
    41521 acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg gcaaagattg
    41581 accaattggc attgaacaga ccgccagacg gtggccgtcg gttgccacgg tgcgcagttc
    41641 ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct gatgcgccat
    41701 cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc tgcggcaggg taaattcgac
    41761 ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag acagcgaaaa
    41821 acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg caatttccgc
    41881 gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg tcgtcgctcc
    41941 tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga gatcagtacc
    42001 ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa tcggtagcgt
    42061 aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat gctcacgttc
    42121 tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa aaatcttctt
    42181 tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga agcaccgtcg
    42241 tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta gtcagctctt
    42301 tcgctagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga cgcttggaaa
    42361 ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg
    42421 tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg aagtcgatgg
    42481 tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc ccttccagct
    42541 cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcaccc ggcaaacgaa
    42601 tgtcattttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc tctggcggtt
    42661 cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca acgccgttga
    42721 tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc agcagggcgt
    42781 tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac tgaatatcgt
    42841 cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt
    42901 gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc actttggcat
    42961 tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt ttacccagac
    43021 ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc gccacctggc
    43081 aagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca aacgtgtgtt
    43141 tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta tcccaacctg
    43201 agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga
    43261 cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac
    43321 gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata ttattaaggt
    43381 acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc agcgtgttat
    43441 cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct ggtaactcat
    43501 cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac tccactcgaa
    43561 caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac
    43621 cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc aagatcctgc
    43681 aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct gtttctcaca
    43741 gattttccct atttatccac aggactttcc agaactcgct aagtgtaaac gatcctgccg
    43801 cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga caaaaattgg
    43861 cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca gcccgtataa
    43921 tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat tttccctgcg
    43981 aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggtg tgtacaatta
    44041 ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca tttttcggcg
    44101 gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca tgaaacgcac
    44161 ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc gtatggctac
    44221 taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt
    44281 ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg gagttacgct
    44341 tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct ggcacgccgc
    44401 aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg
    44461 ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgcctg acgcgtgaaa
    44521 gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag
    44581 gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc
    44641 actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac gcctgattag
    44701 tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg gaattgaggc
    44761 attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg
    44821 ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg ataccagaga
    44881 acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc
    44941 atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca acagaccacg
    45001 cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc cagtggccag
    45061 gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac ccgtggtggt
    45121 gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac ccagccgttc
    45181 cagctgttgg aaacttcacc gcagtttatt tatcaggcac agagcggtct gaccggtcgt
    45241 gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga aaaagacgct
    45301 tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac cgacgcggca
    45361 ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt caacgtcaac
    45421 tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg tcagttgaag
    45481 caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc actgcacacc
    45541 ttccgtggcg cggcgtactc cacgcctgac gagaagtatg agaaatacaa gttcgatacc
    45601 attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc gatgctgcaa
    45661 cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt ctataccgct
    45721 aatctgggta acggcatcgt cgctatcggc tataaatctc agccggtact ggttcagcct
    45781 ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca ggacaaaatg
    45841 gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg gttcatctct
    45901 cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg gggcttctcc
    45961 attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa agcgcagtac
    46021 acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg
    46081 ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc tgtacaaagc tgagaaggtt
    46141 aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct ggcgttgtac
    46201 tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg gatccacgac
    46261 ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac gatgttcttc
    46321 attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat catgaccttt
    46381 atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt actgtactat
    46441 atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg tctggaaaaa
    46501 cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa
    46561 gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg cgcatccgcc
    46621 acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca ggccacgcct
    46681 ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg tgaagttgcc
    46741 gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc gtttaaagac
    46801 gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc gaactcgttc
    46861 accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct cgacctgctg
    46921 ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga gttttccgaa
    46981 cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc cgatcttatc
    47041 gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg cgcattctcc
    47101 gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt cgaagcggca
    47161 attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga agcccagctt
    47221 aatgacgtga tggccgatct cgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg
    47281 cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg ccggtaaatc gagcctgtta
    47341 aacgcgctgg cgggccgtga agcggcaatc gtaaccgata tcgccggaac cacgcgtgac
    47401 gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga cactgccggg
    47461 ctacgtgagg ccagtgacga agtggaacgt attggtatcg agcgcgcgtg gcaggaaatt
    47521 gaacaggccg accgcgtgct gtttatggtc gatggcacca cgacagacgc cgttgatccg
    47581 gcagagatct ggccggaatt tatcgcccgt ctgcccgcga aactgccgat caccgtggta
    47641 cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa cggtcacgcg
    47701 ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa ccatctcaaa
    47761 cagagcatgg gctttgacac caatatggaa ggcggcttcc tggcgcgccg tcgccactta
    47821 caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact gttgggagcc
    47881 tgggcaggtg aactgctggc ggaagagttg cgcctggcac agcagaactt aagcgaaatc
    47941 accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt ctgtattggt
    48001 aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc ttcattgtta
    48061 gcactcctgt tatgcctcaa ccctttttta aacattaaaa ttcttacgta atttataatc
    48121 tttaaaaaaa gcatttaata ctgctccccg aacgattgtg attcgattca catttaaaca
    48181 atttcagaat agacaaaaat t
//
LOCUS       DAJTNP010000022        47955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTNP010000022 DAJTNP010000000
VERSION     DAJTNP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO160_001361"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO160_001361"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5523547.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO160_001362"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO160_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5523548.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO160_001363"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO160_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5523549.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO160_001364"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO160_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5523550.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO160_001365"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO160_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5523551.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO160_001366"
     CDS             5377..5835
                     /locus_tag="OO160_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5523552.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO160_001367"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO160_001367"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5523553.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO160_001368"
     tRNA            6887..6971
                     /locus_tag="OO160_001368"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO160_001369"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO160_001369"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO160_001370"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO160_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5523554.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO160_001371"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO160_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5523555.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO160_001372"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO160_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5523556.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO160_001373"
     CDS             complement(10252..13995)
                     /locus_tag="OO160_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5523557.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRKRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO160_001374"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO160_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5523558.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO160_001375"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO160_001375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5523559.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO160_001376"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO160_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5523560.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO160_001377"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO160_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5523561.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO160_001378"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO160_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5523562.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO160_001379"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO160_001379"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5523563.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO160_001380"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO160_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5523564.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO160_001381"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO160_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5523565.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO160_001382"
     CDS             23392..23499
                     /locus_tag="OO160_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5523566.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO160_001383"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO160_001383"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5523567.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO160_001384"
     CDS             complement(24538..25347)
                     /locus_tag="OO160_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5523568.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO160_001385"
     CDS             complement(25351..25743)
                     /locus_tag="OO160_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5523569.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO160_001386"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO160_001386"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5523570.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO160_001387"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO160_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5523571.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO160_001388"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO160_001388"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5523572.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO160_001389"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO160_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5523573.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO160_001390"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO160_001390"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5523574.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO160_001391"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO160_001391"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5523575.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO160_001392"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO160_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5523576.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO160_001393"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO160_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5523577.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO160_001394"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO160_001394"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5523578.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO160_001395"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO160_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5523579.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO160_001396"
     CDS             36790..39657
                     /locus_tag="OO160_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5523580.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO160_001397"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO160_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5523581.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO160_001398"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO160_001398"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5523582.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO160_001399"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO160_001399"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5523583.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO160_001400"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO160_001400"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5523584.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO160_001401"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO160_001401"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5523585.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYRDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO160_001402"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO160_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12042 a        12342 c        11740 g        11831 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaaa ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gcttgcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgttcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcattac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct accgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaat
//
LOCUS       DAJTNP010000023        47602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTNP010000023 DAJTNP010000000
VERSION     DAJTNP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            253..735
                     /gene="sseA"
                     /locus_tag="OO160_001403"
                     /pseudo
     CDS             253..735
                     /gene="sseA"
                     /locus_tag="OO160_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1554..2330)
                     /gene="sseB"
                     /locus_tag="OO160_001404"
     CDS             complement(1554..2330)
                     /gene="sseB"
                     /locus_tag="OO160_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5523586.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2472..3755)
                     /gene="pepB"
                     /locus_tag="OO160_001405"
     CDS             complement(2472..3755)
                     /gene="pepB"
                     /locus_tag="OO160_001405"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5523587.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3814..4014)
                     /gene="iscX"
                     /locus_tag="OO160_001406"
     CDS             complement(3814..4014)
                     /gene="iscX"
                     /locus_tag="OO160_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5523588.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(4026..4361)
                     /gene="fdx"
                     /locus_tag="OO160_001407"
     CDS             complement(4026..4361)
                     /gene="fdx"
                     /locus_tag="OO160_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5523589.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4363..6213)
                     /gene="hscA"
                     /locus_tag="OO160_001408"
     CDS             complement(4363..6213)
                     /gene="hscA"
                     /locus_tag="OO160_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5523590.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6230..6745)
                     /gene="hscB"
                     /locus_tag="OO160_001409"
     CDS             complement(6230..6745)
                     /gene="hscB"
                     /locus_tag="OO160_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5523591.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6841..7164)
                     /gene="iscA"
                     /locus_tag="OO160_001410"
     CDS             complement(6841..7164)
                     /gene="iscA"
                     /locus_tag="OO160_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5523592.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7181..7567)
                     /gene="iscU"
                     /locus_tag="OO160_001411"
     CDS             complement(7181..7567)
                     /gene="iscU"
                     /locus_tag="OO160_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5523593.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7595..8809)
                     /gene="iscS"
                     /locus_tag="OO160_001412"
     CDS             complement(7595..8809)
                     /gene="iscS"
                     /locus_tag="OO160_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5523594.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8921..9409)
                     /gene="iscR"
                     /locus_tag="OO160_001413"
     CDS             complement(8921..9409)
                     /gene="iscR"
                     /locus_tag="OO160_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5523595.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9679..10419)
                     /gene="trmJ"
                     /locus_tag="OO160_001414"
     CDS             complement(9679..10419)
                     /gene="trmJ"
                     /locus_tag="OO160_001414"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5523596.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10538..11341
                     /gene="suhB"
                     /locus_tag="OO160_001415"
     CDS             10538..11341
                     /gene="suhB"
                     /locus_tag="OO160_001415"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5523597.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11486..12340
                     /locus_tag="OO160_001416"
     CDS             11486..12340
                     /locus_tag="OO160_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5523598.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12531..13811
                     /gene="csiE"
                     /locus_tag="OO160_001417"
     CDS             12531..13811
                     /gene="csiE"
                     /locus_tag="OO160_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5523599.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13803..14942)
                     /locus_tag="OO160_001418"
     CDS             complement(13803..14942)
                     /locus_tag="OO160_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5523600.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15102..15992)
                     /gene="hcaR"
                     /locus_tag="OO160_001419"
     CDS             complement(15102..15992)
                     /gene="hcaR"
                     /locus_tag="OO160_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5523601.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16128..17489
                     /locus_tag="OO160_001420"
     CDS             16128..17489
                     /locus_tag="OO160_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5523602.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17486..18004
                     /locus_tag="OO160_001421"
     CDS             17486..18004
                     /locus_tag="OO160_001421"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5523603.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            18004..18324
                     /locus_tag="OO160_001422"
     CDS             18004..18324
                     /locus_tag="OO160_001422"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5523604.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18321..19133
                     /gene="hcaB"
                     /locus_tag="OO160_001423"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18321..19133
                     /gene="hcaB"
                     /locus_tag="OO160_001423"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5523605.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19143..20365
                     /locus_tag="OO160_001424"
                     /pseudo
     CDS             19143..20365
                     /locus_tag="OO160_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20462..20884
                     /locus_tag="OO160_001425"
     CDS             20462..20884
                     /locus_tag="OO160_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5523606.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20932..21804)
                     /locus_tag="OO160_001426"
     CDS             complement(20932..21804)
                     /locus_tag="OO160_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5523607.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21816..22910)
                     /locus_tag="OO160_001427"
     CDS             complement(21816..22910)
                     /locus_tag="OO160_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5523608.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22943..23941)
                     /locus_tag="OO160_001428"
     CDS             complement(22943..23941)
                     /locus_tag="OO160_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5523609.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23966..25477)
                     /locus_tag="OO160_001429"
     CDS             complement(23966..25477)
                     /locus_tag="OO160_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5523610.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25493..26487)
                     /locus_tag="OO160_001430"
                     /pseudo
     CDS             complement(25493..26487)
                     /locus_tag="OO160_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26584..29685)
                     /locus_tag="OO160_001431"
     CDS             complement(26584..29685)
                     /locus_tag="OO160_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5523611.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29692..29865)
                     /locus_tag="OO160_001432"
     CDS             complement(29692..29865)
                     /locus_tag="OO160_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523612.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29983..31176
                     /locus_tag="OO160_001433"
     CDS             29983..31176
                     /locus_tag="OO160_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5523613.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31374..32627)
                     /gene="glyA"
                     /locus_tag="OO160_001434"
     CDS             complement(31374..32627)
                     /gene="glyA"
                     /locus_tag="OO160_001434"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5523614.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32954..34144
                     /gene="hmpA"
                     /locus_tag="OO160_001435"
     CDS             32954..34144
                     /gene="hmpA"
                     /locus_tag="OO160_001435"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5523615.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34189..34527)
                     /gene="glnB"
                     /locus_tag="OO160_001436"
     CDS             complement(34189..34527)
                     /gene="glnB"
                     /locus_tag="OO160_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5523616.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34588..35922)
                     /gene="glrR"
                     /locus_tag="OO160_001437"
     CDS             complement(34588..35922)
                     /gene="glrR"
                     /locus_tag="OO160_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5523617.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35912..36625)
                     /gene="qseG"
                     /locus_tag="OO160_001438"
     CDS             complement(35912..36625)
                     /gene="qseG"
                     /locus_tag="OO160_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5523618.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36790..38217)
                     /gene="qseE"
                     /locus_tag="OO160_001439"
     CDS             complement(36790..38217)
                     /gene="qseE"
                     /locus_tag="OO160_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5523619.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38775..42662)
                     /gene="purL"
                     /locus_tag="OO160_001440"
                     /gene_synonym="purI"
     CDS             complement(38775..42662)
                     /gene="purL"
                     /locus_tag="OO160_001440"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5523620.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42921..44477
                     /gene="mltF"
                     /locus_tag="OO160_001441"
     CDS             42921..44477
                     /gene="mltF"
                     /locus_tag="OO160_001441"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5523621.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44474..45010)
                     /gene="tadA"
                     /locus_tag="OO160_001442"
     CDS             complement(44474..45010)
                     /gene="tadA"
                     /locus_tag="OO160_001442"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5523622.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(45035..45670)
                     /gene="yfhb"
                     /locus_tag="OO160_001443"
     CDS             complement(45035..45670)
                     /gene="yfhb"
                     /locus_tag="OO160_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5523623.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45879..46727
                     /locus_tag="OO160_001444"
     CDS             45879..46727
                     /locus_tag="OO160_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5523624.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(47066..47602)
                     /locus_tag="OO160_001445"
                     /pseudo
     CDS             complement(47066..47602)
                     /locus_tag="OO160_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11349 a        13044 c        11978 g        11231 t
ORIGIN      
        1 tcgtaatcgc tggattcggt cattctgtct cccttgtata tgggttaacc agataaccac
       61 ggggagcagg taccggcatc cacgttacag acggtcccgg caaaagtgcc tgacctgacc
      121 cctattagcg gttaccagcg ccccaccaga cccggtgaca tcaccccccg gatcatggac
      181 gactgggggc agtaatcatg ccgggtctgg ctggctaaca ccccattata aggggtacga
      241 ataaagtaac ggggacttgc aggctggcag cgcgatgacc tgctattaga agaaggtgca
      301 gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc ctgaagcagt ggtgaaagta
      361 accgatgtgt tattggcaag ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct
      421 gcacgtttta acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg acatattccc
      481 ggtgcattga acgttccgtg gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat
      541 gaactggatg cgatattttt tggtcgcggc gtcagctatg acaaaccgat tatcgtcagc
      601 tgcggctctg gtgtaacggc agccgtggtt ttgttagcac tcgcgacgct ggatgtgcca
      661 aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg cgcgggcaga tttaccggtt
      721 gagccagtga aataagtata ttacaggcaa taaaaaaccg ccgaatttgg cgttttttta
      781 ttgctggtct ggttcgcggc ctttccagca ggttgacttg tgttacatga gcaacgcagg
      841 tgcttcacag caaaacaata ctcaccagta actctctttt tgtcaagcaa aagagagtaa
      901 ttattgttta tttagcgtat tatcgacacc ggccctttcc gccgtgttcg gtaataaaat
      961 aacctggctt attagtccga attcagacaa atataaacaa atcctgctca aaattaaaaa
     1021 ttctaaccgg taaaagatat tacttaaaca tgtaaattta ctttccttta aaaaacaaaa
     1081 aaccgccaaa atcaggcggt tatttgttgc tggtccggtt cgcggccttt ccagcaggtt
     1141 gtattaccgt agtaatgcaa gcgcgtctca gcggagacaa tactcgccag taactctctt
     1201 tttgtcaagc aaaagagagt tattattgtt ctgttagtgt attatccact gcggcccttt
     1261 ccgccgtctc gcaagcgggc gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa
     1321 gcgcgtctca gcgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct
     1381 tcgcatggac cgcacaatga agatacggtg cttctgtatc gtacttattg tttctggtgc
     1441 gctgttttcc caagtaaata ataaccggag tctctccggc gacaatttac tggtggttaa
     1501 caaccttcag agcagcaagt aagcccgaat gccgcccttt agggcggcat attttagatt
     1561 atccggtttt gtttaaagtc acgcaaaaaa ccaccccagc gacgttcata gaatggcgca
     1621 atatgttcgg taataaagtg gctaattcct ttttcccctt ttttcacctg acaaatatcg
     1681 attggttcat cgccaggtaa tgtatcggtc gctacacttc ccgtcgcctg aataatttct
     1741 tcgatatcac catcggcttc aatgccaata agtaaattag gctgtgcctc ttcgttctct
     1801 ttaattgaac aaataaaagc acgcttcacc ggcttaatgg ttttaaataa ggtggtgagt
     1861 gaatcaatca tttgtgctgg cggctctgcg acttccgata atatcagcga ttcaccgcct
     1921 tccaggattt cctggctgct cagcggattt ccctcttcac caatcaacaa actgatttca
     1981 cgcggcataa attctttacc ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg
     2041 gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc
     2101 tgttgaagcg cttctaacga ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc
     2161 cagtgctgta aatcaagcgc actatcttca accacagcct cgccctgcgc cgccgtacca
     2221 ggcacccaga cggtggattc cagtagagta cggaaaaagg ccgggcggtg cgccggttcg
     2281 gttgctgctt tttccagcag gtcttcaagt tcgttttttg tttcggacat aagaaccaca
     2341 attcattcaa cgttcggggc gcaaatgcct gatgcgctac gcttatcagg cctacaaggg
     2401 attcgcaatt tgttgaattt gcagaatttg taggccggat aaggcgttta cgccgcatcc
     2461 ggcatattag tttacgccgt taacagatta gctatcgtgc gcacaccaag tcccgtagcg
     2521 cccgcagacc actgttcaac cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc
     2581 cagccttgct gatagttctc aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct
     2641 gccggatacg ccgcgcttcc ggtattgttc agttcggcaa agttagacgg cagctggctg
     2701 cggtggaatt ccgccagcgg cagacgccag aatggttcgt tctcttgtga ggcactcgcc
     2761 agtaaacgac cggcaagcgc atcgtcaaaa ctgaacagcg cgtgataatc attacccagc
     2821 gcagttttcg ccgccccggt gagggtcgcc gcatcaatga tcatttccgg tttctgcgca
     2881 ctggcatcaa tcagaccatc ggcaagcacc agacgccctt cggcatcagt gttcatcact
     2941 tcaacttttt taccgttgcg ataggtgatg atatcgccca gcttgaacgc attgccgcta
     3001 atcaggttat ccgcacagca gaggaacagc ttcacgcgct tgttcagtcc gcgcataatg
     3061 gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca tgtccgactt catcgagtcc
     3121 ataaacgcag tctgtttgat gctgtagccg ccggagtcaa aagtgatacc tttacctacc
     3181 aggcacgcgt acaccggtgc ttctttatcg ccagttgggt tgtaatccag cgccagcaat
     3241 accggagaac gttctgaacc gcgcccgaca gtgtgcagcc ccatataacc ttgctcacgc
     3301 agatcttcgc ctttggtgat ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga
     3361 tcaacagcac gctgtgccag ttgcgatggt cccaattctt ctgccggtgc gttgatggtg
     3421 tcacgcaccc agtcgatgat catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc
     3481 agatccggcc actccacttt acgcgtgcct ttcgggcctt tataaccttg ccagaatgcc
     3541 cagcagcgat ccgcatccca gccttcaccg cttaactgaa catgcttgat gcccagaccg
     3601 tcaatcttgc gggccgcacg ctggatcagc cccagatcgt ctgccccgtt caaatgcagg
     3661 gtaatgccgt cattattaat gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt
     3721 tgggtagaga gggtaatctt catcgcttct gtcattttag ttatccttat tcgtttaaca
     3781 gcaaaacggg ctacccccag gcagcccgtt cattcactcg gcttcgtcca gccagactaa
     3841 caaaatcgct tcgaggattt tctcgttgga tgcctgcggg tcgtcgtcga aatcttccag
     3901 atcgcaaatc cactgatgca tatcggtgaa tcgaaccgtt ttcggatcaa gatcgggata
     3961 cgcatcgtac agtgcttcgc caatttcgcg gctatcggtc cacttaagtc ccatactaac
     4021 ctctgttaat gctcacgcgc atggttgata gtgtaacgcg ggatttcgac cactaaatct
     4081 tcgtcggtga cgcgcgcctg gcagcttaaa cggctttccg gctccagtcc ccaggctttg
     4141 tccagcatgt cgtcttcctg ctctgagctt tccggcagtg agtcaaaacc ttcacgaacg
     4201 atgcagtggc aggtggtgca agcacaggat ttttcacagg cgtgttcaat ctcaataccg
     4261 ttacgcagcg ctgcgtcgag aatggtttca ccgctattag cttccagaac agcgccatca
     4321 gggcagaggt cctgatgagg caaaataaca atctttggca tattaaacct cgtccacgga
     4381 atggcctttc agcgcacgac gaaccgactg gtccatgcgg cgagcggcga aatcctgggt
     4441 ttgtttgtct acgtttttaa tcgcttgttc gatggcgtca acatcatcgc cctgcgccac
     4501 ttcactcagg tgagcggcag cattgtcaat gacctgacgc tctgcggcgc ttaacagcgc
     4561 ggcatcagca gccagcgcgc cgtgcagact ttccagcaca cgcgccgctt ctactttttg
     4621 ttctgccagc atacgggctt ttacgtcctg ctcggcatag ctcattgagt ctttgatcat
     4681 cgaagcgatt tcgctatcgg tcagaccgta agacggtttg acctgaatag acgcctcaac
     4741 gccggtggat ttctccatcg ccgtcacgct caaaagaccg tcggcatcga cctggaacgt
     4801 cacgcgaata tgcgcaccgc cagccggtag cgccggaata ccacgcagcg caaaacgcgc
     4861 cagtgagcgg cagtcctgca ccagttcgcg ctcaccctgc attacatgga tagacatcgc
     4921 cgtctgacca tctttaaagg tggtgaaatc ctgagcgcgg gccaccggaa tagtggtatt
     4981 acgcggaatc actttctcca ccaggccgcc catcgtttcg aggcccagcg acagtgggat
     5041 cacatcaagc agcagcattt cgctgtctgg cttgttaccc accagaatat ccgcctgaat
     5101 cgccgcgcca atagcgacga ctttatccgg gtcgatggaa gtcagcggtg gacgaccgaa
     5161 aaattcgcct acccgttcac gcaccagcgg cacgcgagta gaaccgccca ccatcaccac
     5221 ttccagcact tcgtcagctt ctacacccgc gtctttcagc gcgcgacgac aagccagtaa
     5281 ggttcgtttt accagtggcg cgatcagttc attgaattgt tcacggctga tttcgccctg
     5341 ccagcccgca acgttaacgg tcacggagtc cgcatcgctc agcgcgattt tggctgcaat
     5401 ggtggcatcc agcagttcac gctgaacgcg gttatcgcta cgatcaggaa tgtccgcctg
     5461 ctcgcgaatg taatccgcca gcagatggtc gaaatcatcg ccgccgagcg cggaatcacc
     5521 gccggttgcc agcacttcaa acacgccgcg acttaagcgc agaatggaaa tatcaaacgt
     5581 cccgccaccg aggtcataaa cagcgatcac gccttcctga ccggaatcca gcccgtaggc
     5641 gatagccgca gcagtcggtt cgttaagtaa gcgcagaaca tgcaaccccg ccagacgcgc
     5701 cgcgtctttg gtgccctgac gctgggcatc gtcaaagtac gccggaacgg tgataactac
     5761 accatccagc tcgcctgcca gggcttcagt tgcccgcgcc gccagtgctt tgaggatgtc
     5821 cgcagaaacg cgcaccgggt tcagtagccc cgccgccgtt tcaatcatcg gcaggccgtt
     5881 ttcgctggcc tggaattgat aaggcagatg cggatagcgt tgctggatat cagccagcga
     5941 gcgccccatc aggcgtttaa cagaactaat ggtgttggcg gtgtcaagcg ctgcgttagt
     6001 acgcgcgtca taacccaccg aatgcccttg ctgttgatag tgaacaacag atggcagcag
     6061 gtgacggcct tcatggtcgg ctaacgtttc ggcctgaccg ctgcgcactg tcgccaccag
     6121 cgagttggtt gtgcccaggt caataccagc cgccagacga cgctgatgcg gcgcagcact
     6181 caaaccaggt tcactaattt gtaataaggc catgtttagc ttccagaaat taaaaatcga
     6241 gcagtttttc ttcgagttgt tcggcactgc tccgcagttt atcgagaaaa cgcagcttac
     6301 gcaccgtatc cgccgccgcg tcccacgcct cgttgtctaa ctgttcaacc atcaactgat
     6361 ggcgggtatc aaacatcttt ttcacacgtt tgataaagct ttccagccgc gcttcatctt
     6421 tcgcctgttc gatctcgtcc agctcttcgc gcagctccaa ctgttccatc aggaacgcgg
     6481 tgtcgcgcac agtatgctgc tcgctggcga gatcaaaacc gtgtaaagaa agcaaatatt
     6541 ccgcgcgcat taacggatga cgcagcgttt gccaggcctg gttaatggtt gcagattgct
     6601 gtacggcggc gagttgttcc gccaggcttc cgctggcgaa tttatcagga tgatactgac
     6661 gttgtagatc ctgaaaacgc aggctcagtg cctgggtatc gagttgataa cgggcaggca
     6721 agccaaagag ggtgaagtaa tccataacat tctcagggct gcgatccgcc gcgataacgg
     6781 cgaatcaggt aaaacaaacc ccacgcgcag gcgaccacgg tggggttatc ggtatgcgca
     6841 tcaaacgtgg aagctttcgc cgcaaccaca ctcatcttta acgttcgggt tggtgaattt
     6901 aaacccttca ttcaggcctt cttttacgaa gtccagctgc gtaccgtcca gaaattgcag
     6961 gcttttgcca tcgaccacga ctttcacgcc tttgtcttca aacacgatgt cttccggcgt
     7021 cggttcgtca acaaattcca gtacataagc catacctgaa catccggagg ttctcacgcc
     7081 cagacgcagg ccaaaccctt tgccgcggtt agccagaaag gtatttactc gcgctgctgc
     7141 actgtcgctc agtgtaatcg acataaccaa acctcaactc ttattttgct tcacgtttgc
     7201 ttttatagtc cgcaatggcg gctttgatcg cgtcttctgc cagaatagaa cagtgaattt
     7261 tcaccggcgg cagttcaagt tcttcagcaa tatcggtgtt tttgatcgcc tgcgcttcgt
     7321 cgagagactt ccctttcacc cattcggtga ccagggagct ggaagcgata gcggaaccgc
     7381 agccgtaagt tttaaaacgc gcgtcttcaa tgataccttc atcgttgact ttaatctgca
     7441 acttcattac gtcgccacag gccggtgcac ccaccatgcc gctgccgacg ttctcgtcgt
     7501 tgttatcaaa ggaacccacg ttacgcggat tctcgtaatg gtcgataact ttttcgctgt
     7561 aagccattat aaattctcct gattccgata ccgattaatg atgagcccat tcgatgctgt
     7621 tcagatccac gccctgcttg tacatttccc acagcggaga aaggtcacgc agacgaccga
     7681 tggatttacg aactaactcg atggtgtagt cgatctcttc ttcagtagta aaacgaccta
     7741 aagagaaacg gatagagcta tgtgccagct cgtcgttcag ccccagcgcg cgcagcacgt
     7801 aggacggttc gaggcttgct gacgtacagg cggaacctga agaaactgcg aggtctttca
     7861 gcgccataat cagcgactca ccttcaacgt agttgaagct gacgttgaga atgttcggcg
     7921 caccgtgttc caggtcaccg ttcaggtaaa cttcttcgat atctttgatg ccgttccaca
     7981 gacggttacg caggccgcgc agacgttcca tctcggtcgc catctcttct tttgcgatgc
     8041 gataggcttc gcccattccg acgatctggt gaacaggcag agtgccggaa cgcataccgc
     8101 gctcgtgacc gccgccgtgc atttgcgctt cgatgcgtac acgcggttta cgacgcacat
     8161 acagcgcacc gatacctttc gggccataga ttttgtgacc ggagaaagac atcaggtcaa
     8221 ctttcaactg gctcaggtcg attggcagtt tgcccacgct ctgggttgca tcaacgtgat
     8281 agataatgcc acgagcacgg cacatttcgc cgatagccgc gatatcctgc accacgccga
     8341 tttcgttatt tacgtgcatg atggaaacaa ggatggtgtc gtcacgcatc gccgcttcga
     8401 gttctttcag gtcgataata ccgttacgct gcggtgccag gtaggtgact tcgaaacctt
     8461 cgcgctccag ctgacggcaa gtatccagta ccgctttgtg ttcggttttg ctggtgatga
     8521 tgtgcttgcc ttttttctga taaaagttgg ctgcaccttt gatcgccagg ttgtcagatt
     8581 cggttgcacc agaggtaaag acgatttcac gcggatcagc gccgaccaga tcggcaatct
     8641 gattacgggc gatatctacc gcttcttcag cctgccagcc gaaacggtga gaacgagagg
     8701 ccgggttacc aaaggttccg tccatcgtca tgaactgcat cattttctcg gcaacacgcg
     8761 ggtccaccgg cgtggttgcg gagtagtcga gataaatcgg taatttcatt gctctataaa
     8821 ctccgtacat cactcaatgc aaggaatcag gctaccggct ggatgtacga ccgtgtttac
     8881 ggagtattta gcactccggc ctgattctga attcttttta ttaagcgcgt aacttaacgt
     8941 cgatcgcgtc ttgtgtgcgg gtgcgtggcg cgtcgtgagt atgctgacga ccagacacat
     9001 ccagcacttc ctggttatta accagttcgc ctaaagtaat gttgttgaga aaaccggtga
     9061 gacggtcgct caaatcacgc cacagcgcgt gggtcaggca tttatcgccg ccctggcagc
     9121 cgcctttacc ctgacaacgg gtggcatcta cagattcgtc aacggcgcta atcacttcgc
     9181 caacggcgat gctgctggca tctttgccta acagataacc accgcctggt ccacgtacgc
     9241 tggaaaccag accattttta cgcagacggg aaaacagttg ttccagataa gaaagggaaa
     9301 ttccctgacg ttcggaaata tcagccaacg gtaccgggcc cgcttcagag ttgagcgcaa
     9361 cgtcaagcat tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg tcttacttca
     9421 cctcaaactc gcccctgccc ggggtttttt attgtaaaag tgggggtatt gcatagcagg
     9481 gtcaagtctg acattcccga gtaaattggt caactattta cttgactgat ttagtcgggt
     9541 atttaacctt cagtgccatt tttttatcgt ggcgtttgta cctgttgtcg gatgcggtgt
     9601 gaacacctta tccgacctac gattctgtac attgtaggcc tgataagacg cgttaagcgt
     9661 cgcatcaggc aaccactgtt attcggcctt gttaccttta ttctgctgct caatagaagc
     9721 cagaatcccg cgcaggatat tcaattcctg gctttccggg cgcgcacggg taaacagacg
     9781 gcgcaattta ttcatcacct gccccggatg gttttcacgg ataaaaccgg ttgccagcag
     9841 cgtttgttcc agatgaccat aaaaacgctc cagatcatcg accagcggat acggcgtctc
     9901 ttcatgctcg acctgctcgc cattttcctg agttgccagc caggccatac gcacttcata
     9961 ggcaataacc tgaaccgcca ttgccaggtt cagcgaactg tattccgggt ttgccgcaat
    10021 cgcgacatga taatggcatt tctgcaactc ttcattggtc aagccgacgc gctcacgacc
    10081 aaacaccagc gccaccgggg tatttgccgc ttcagcgacg cttttcagac cgcattcgcg
    10141 cgggtcgagc atcggccacg gcagcgtgcg ggagcgtgcg ctggtgccca ccaccaggct
    10201 acaaccggct aacgcttcgt cgagcgtatc gacgatgtga gcattaccaa tcacatcgct
    10261 ggcccctgca gccagggcaa tcgcctggga gtcgggtttc accagtggat taaccagcca
    10321 cagattggtt aatcccattg ttttcatggc acgggcaaca gaacccatat tgccggtgtg
    10381 tgacgtctcc accagcacaa ttcgaatatt ttgcagcatt gtctttcttc atctaaagat
    10441 tattcacgca tcttatcata aaacgaagac agatgccgat ctcgctgcta tactctgcgc
    10501 cgttttcccg ttctttaaca tccagtgaga gagaccgatg catccgatgc tgaacatcgc
    10561 cgtgcgcgca gcgcgcaagg cgggtaattt aattgccaaa aactatgaaa ccccggacgc
    10621 tgtagaagcg agccagaaag gcagtaacga tttcgtgacc aacgtagata aagctgccga
    10681 agcggtgatt atcgacacga ttcgtaaatc ttacccacag cacaccatca tcaccgaaga
    10741 aagcggtgaa cttgaaggta ctgatcagga tgttcaatgg gttatcgatc cactggatgg
    10801 tactaccaac tttatcaaac gtctgccgca cttcgcggta tctatcgccg ttcgtatcaa
    10861 aggccgcacc gaagttgctg tggtatacga tcctatgcgt aacgaactgt tcaccgccac
    10921 tcgcggtcaa ggcgcacagc tgaacggcta ccgtctgcgc ggtagcaccg ctcgcgatct
    10981 cgacggtacc attctggcga ccggcttccc gttcaaagca aaacagtacg ccactaccta
    11041 catcaacatc gtcggcaagc tgttcaacga atgtgcagac ttccgtcgta ccggttctgc
    11101 ggcgctggat ctggcttacg tcgctgcggg tcgtgttgac ggtttctttg aaatcggtct
    11161 gcgtccgtgg gatttcgcgg caggcgagct gctggttcgt gaagcgggcg gcatcgtcag
    11221 cgacttcacc ggtggtcata actatatgct gaccggtaac atcgttgctg gtaacccgcg
    11281 tgttgtgaaa gccatactgg cgaacatgcg tgacgagtta agcgacgctc tgaagcatta
    11341 atgactcagg cgggtgatat cactcacccg ccctcgcctt tcaggcgcta ttccgaaata
    11401 cttcctcacc gctttacttt ctttcgtcac tctcccacca tctttccccg attaatggat
    11461 gataaagaag tcgcaaggaa aggttatggc actgccagtg aacaaacgcg ttcccaaaat
    11521 tctgtttatt ctctttgttg ttgccttctg cgtttattta gtgccgcgcg ttgccatcaa
    11581 cttcttctat tatcctgacg ataaaattta cggtcccgac ccctggtcgg cggaatccgt
    11641 cgaatttacg gctaaggacg gtactcgtct gcaaggctgg tttattcctt cttcgacggg
    11701 ccctgctgac aacgccatcg caaccatcat tcatgctcac ggcaatgccg gaaatatgtc
    11761 cgcccactgg ccgctggtca gttggttacc tgagcgtaat ttcaacgttt ttatgtttga
    11821 ttatcgcggg tttggtaaat caaaaggcac tccgtcccag gccggattgc tggacgatac
    11881 gcaaagcgcc atcaatgtgg tgcgccatcg cagtgatgta aacccacaac agctggtgct
    11941 gttcgggcag agtattggcg gggcgaatat tcttgctgtt attggtcagg gtgatcgtga
    12001 aggcatacgt gcggtgatcc tcgactccac atttgcctct tatgcaacca tcgccaacca
    12061 gatgatcccc ggcagtggct acttacttga tgagagttac agcggcgaaa attacatcgc
    12121 cagcgtcagc ccgatcccgc ttttactcat tcacggtaaa gccgatcacg ttatcccatg
    12181 gcagcacagc gaaaagttgt atagcctggc aaaagagcca aaacggttga tcctaatccc
    12241 ggatggcgaa cacattgatg ctttttccga tcgtcacggc aatgtttatc gcgaacagat
    12301 ggtggacttt atccttagcg cgttgaatcc gcagaactaa cccatgatcg ctagcacgat
    12361 aatcattcac aaaaccacct taagacatgc taatccactg gtcagaacag tttaaaatga
    12421 gaaaaattct gtgacgcttg ccaacatttc tgatgattag cattcccttc gccatttccc
    12481 tgagcaaact ttagctattc ttatcaatta tgcttatggg agatctacag atgatgccta
    12541 cgcttgctcc accatctgtc ctttcggctc cccagcgccg ctgccagatc ttgctgacgc
    12601 tctttcagcc ggggttgacc gccaccatgg caaccttcag cgagcttaat ggtgtggatg
    12661 atgatattgc cagtcttgat atcagcgaaa caggacggga gatcctgcgc tatcatcaac
    12721 tcacactgac gactggttat gacggtagct accgggttga aggtacagtg cttaaccaac
    12781 gtttgtgttt atttcactgg ctacgacgtg gtttccgtct gtgtccgtca tttattacca
    12841 gccaattcac ccccgccctg aagagtgaac tgaagcggcg cggaattgcg cgtaactttt
    12901 acgacgatac caatctacaa gcgttagtga atctctgctc ccgacggctg caaaaacgct
    12961 ttgaatcgcg cgatattcat ttcctgtgtc tgtatctgca atattgtttg ctgcagcacc
    13021 acgctggaat tacgcctcag tttaatccgc tccaacgtcg ctgggcggaa tcctgccttg
    13081 aatttcaggt agcgcaggaa attggacgcc actggcagcg tcgggcgctc cagcatgtac
    13141 cgcctgatga gccactgttt atggcattac ttttttccat gttgcgggtt cccgatccac
    13201 tgcgggatgc gcatcagcgg gacagacaat tgcgtcagtc tatcaaacgt ctggtaaacc
    13261 attttcgtga gctgggaaat gttcgttttt atgatgaaca ggggttatgt gatcagcttt
    13321 atatccacct cgcccaggcg ttaaatcgca gtttgtttgc catcggtatt gataataccc
    13381 tgccggaaga gttcgccaga ctgtatccac gcctggtacg caccacccgc gcggcgctgg
    13441 ccggatttga aagtgaatac ggcgtccatc tttctgatga ggaaagtggt ctggtcgcgg
    13501 tgattttcgg tgcctggcta atgcaggaaa acgatctgca tgaaaaacag attattctac
    13561 tgaccgggaa tgatagcgag cgagaagcgc agattgagca gcagttacgc gaactaacgt
    13621 tactgccgct caacattaag catatgtcgg taaaggcatt tttgcagaca ggcgctccgc
    13681 gtggcgcggc actgattatt gcgccttata ccatgccgtt accgctcttt tcaccaccgc
    13741 tgatctatac ggacctgacg ctgacaacac atcaacagga gcagatccgc aaaatgctcg
    13801 aatcagcatg agggaacaac tttcgggcgc aaaaacattg ccggaagcgc caccagcgcc
    13861 attacccaga acacgccgtg gcccagatat tgatacagga aaccggcgaa aacggtcatg
    13921 atagcgatac tgccgcccat cgcgacggca gagtaaaccg cctgtaaacg gatgacttcg
    13981 ctaacctggc gagcagcaat ataacgcatg gcggccaggt ggcagaccgt gaaggtgccg
    14041 caatgcagaa tttgcaccac tatcaaccac ggcaacgccg tagttgctcc cataatgccc
    14101 cagcgcacta cgccgcaaat cgccgagatc aacagcatat cgcgagcact acaacggcgg
    14161 aaaagtttat tactcagcgc aaagataatg acttccgcca ccacgcccag cgaccacaaa
    14221 taccccaccg ccgaggccga gtagccagct gcctgccagt aaatggcgct aaaaccgtaa
    14281 taggccgcat gtgccccctg caataaacaa acgcaggcca gaaagcgcca gttctggcga
    14341 accagcgcca accacgcaga ccaaccggtg ctctcctgct ggcggcttgc cccttgtggc
    14401 tgaatcgtcg gacggatgag aaagccgagc agcatggatg ccacgcccaa cgtcaacagc
    14461 acgaggatca cccgataatc aaacatagtg accagtttgc ccgtcagcgc cgagccaatg
    14521 acaaacgcca ccgagcccca cagtcgcact ttgccgtaat caagcgggaa ctgcttttgc
    14581 cacgtattcg ccagtgcatc ggtcaacggt accagcggtg agaaaaagag gttaaagcca
    14641 atcatcacca gcatcagcca cgctacgtgc gcccccgccc agaaggcgac agcaaagaga
    14701 agtggcagca gtgccagcac gcgcaaggcg gaaatcaggc gggaaggatc gctgacgcgg
    14761 ggcgcgatga gcaaactccc gagaaaacgg gcaaccagac ctgcccccaa taacaggccg
    14821 atggtttctg gcgttaaacc aatcccttta agccagacgc tccagaaagg taaaaaaatg
    14881 ccgtaactaa aaaagtatgt gaaatagccg agcgccaacc agcgcgtgga ttgcaaaacc
    14941 atgagtccct cccgtcagga ggcgttagtc tggggttaat cgaatgttgg tgcaagttga
    15001 aaaataagaa tattcgagcc tgcttcccga tgtctgtaac gccagttcac aagcactaaa
    15061 aaactgcgat ccgcgacgca tgttgcaaaa ataatgcgta attatgccgt tacgcttgcc
    15121 aaacgttcct gcacaatggc gatgaaatcg cgtaatgcca gcttcatctc ccctttcttc
    15181 cacgccatca gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa aacaacctgc
    15241 ccggtattaa aattattcat ataaccgggt atcaaagtga cgcccagccc catgcccacc
    15301 agattcatgg tcaccagaat attcgttgcc acctggacga tatttggctg gctgttttct
    15361 tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg aatacgccgg atcggtactg
    15421 acgaaattca cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc taaagggtga
    15481 tcaaccggta acaccaccac taatggttcg tcaaaaagct ccagataatc aatctccggg
    15541 ctataaacag gatggcgcat taagccgacg tcgagttcac cacggcgaat tttttcctcc
    15601 tgttgcgtgg tgattaaact caccagctca atcaaggtgt ctggctgtct gagacgaaac
    15661 atcggtaata cttttggcaa taaattcact tccgccgatg gcacaaagcc aatggttaat
    15721 tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg cattttccgc ttgttcgagg
    15781 attgccagcg cgtcctggag aaaacattct cccgccgccg tcaacgcgac tttgcgctta
    15841 tccctcacca gtaacggaac accgacacag ttttcaagat cgcggatctg gctgcttaac
    15901 gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa agttcagtgc ctgcgccact
    15961 gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc gaaaaaacgt
    16021 ctcagctggt agaaattaac tatttcacat attagcaacc aaccagcaac atccttatgg
    16081 cacaaaaata gaaggtcaat acatcttatc tttcaggatt aaaaaatatg accacaccct
    16141 cagatttgaa catttaccaa ctgattgaca cccaaaacgg tcgggtcact ccgcgtattt
    16201 ataccgaccc ggacatttac caactggagc ttgagcgtat tttcggtcgt tgctggctat
    16261 ttctcgccca cgaaagccag atcccaaaac ccggtgattt ctttaacacc tacatgggag
    16321 aagatgcagt tgtcgtggtg cgccagaaag acggcagcat caaggcgttt ctcaaccaat
    16381 gccgccaccg ggccatgcgt gtgagttatg cagattgtgg caacagtcgc gcctttacct
    16441 gcccgtatca cggctggtct tatggcatta acggcgagtt gatcgatgta ccgctggaac
    16501 ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg gctaaacgaa gttccttgtg
    16561 tggagagtta taaaggactg atttttggca actgggatac cagcgcaccg ggcctgcatg
    16621 attacctggg tgacattgcc tggtatctgg atggcatgct ggatcgtcgc gaaggcggca
    16681 ccgaaattgt cggcggcgta caaaagtggg tgatcaactg taactggaaa ttcccggcag
    16741 agcagttcgc cagtgaccag tatcatgctc tgttcagcca tgcttctgcc gttcaggtat
    16801 taggggcgaa agatgatggc agcgataagc gcctcggtga tggacaaacc gcccgcccgg
    16861 tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga cggtcacggt agcggtttct
    16921 tctttactga aaaaccggat gctaatgtct gggtcgatgg cgcagtttca agctattacc
    16981 gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg cgcctggcgg
    17041 gtcataacaa tatattcccc acgctttcat ggctaaacgg cactgccaca ctccgcgtct
    17101 ggcatccgcg cggccctgat caagttgaag tgtgggcgtt ctgtattact gacaaagccg
    17161 cctccgatga agttaaagcc gcttttgaaa acagcgccac tcgtgctttt ggtcctgctg
    17221 gttttctcga gcaggatgac tcggagaact ggtgtgaaat ccagaaattg cttaaaggcc
    17281 accgcgcccg caacagcaaa ctgtgtctgg aaatggggct tggtcaggaa aagcgccgcg
    17341 acgacggcat tcctggcatt actaactata tcttttcaga aactgccgct cgtggaatgt
    17401 accaacgctg ggccgatctt ctgagtagcg aaagctggca ggaagtgctc gataaaaccg
    17461 ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt tcactggagt tacatcaccg
    17521 cattagccag tttctctttc acgaagccag tctgctggat gactggaaat ttcgtgactg
    17581 gttggcacag ctcgacgaag agattcgtta caccatgcgc accacggtta acgcgcaaac
    17641 acgcgaccgt cgcaaaggcg tccagccgcc gacgacctgg attttcaatg acaccaaaga
    17701 ccagctggag cggcgaatcg cccgtctgga aacgggcatg gcctgggcag aagagccgcc
    17761 gtcacgcact cgtcacttaa tcagcaactg ccaggtaagc gaaaccgaca tcccaaacgt
    17821 atttgccgtg agggtgaatt atctacttta tcgggcacaa aaagagcgcg atgaaacatt
    17881 ctatgttgga acgcgtttcg acaaagttcg ccgtctggaa gatgacaact ggcgcttgct
    17941 ggaacgggat atcgtcctgg atcaagcggt gatcacttcc cataacctga gtgtactgtt
    18001 ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga ggctctccgg
    18061 atagatacct cgcccgtcat cgccttgttc aacgtgggcg gcgagtttta tgccattaac
    18121 gatcgttgca gccatggtaa tgcgtcgatg tcagaagggt atctggaaga tgacgccacg
    18181 gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc gttatgcctg
    18241 cccgccaccg atccgctcac cacttatcca gtacacgttg aaggtggtga cattttcatc
    18301 gacttaccgg aggcgcagcc atgagcgatc tgcataacga gtccattttt attaccggcg
    18361 gcggatcggg attagggctg gcgctggtcg agcgatttat cgaagaaggc gcgcaagttg
    18421 ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt ggcgaacata
    18481 ttctggcggt ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg gtcgatcaga
    18541 tcctgactcg ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc tgggatcaca
    18601 atgcctcact ggttaatact cccgcagaga cgctcgaaac cggcttccac gagctgttta
    18661 acgtcaacgt actcggttac ctgctgggcg caaaagcctg cgctccggcg ttaatcgcca
    18721 gtgaaggcag catgattttc acactgtcaa atgccgcctg gtatcctggc ggcggtggcc
    18781 cgctgtacac caccagtaaa catgccgcaa ccggacttat tcgccaactg gcttatgaac
    18841 tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc gacctgcgcg
    18901 gcccacaggc gctcgggcaa agtgaaacct cgataatgca gtctctgacg ccggagaaaa
    18961 ttgccgccat tttaccgctg caatttttcc cgcaaccggc ggattttacg gggccgtatg
    19021 tgatgttggc atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc aacgctgatg
    19081 cgggtttagc gattcgcggc attcgccacg tagcggctgg gctggatctt taaggaagca
    19141 cgatgaaaga aaaaacgatc attattgtcg gtggcgggca agcggcggca atggctgcgg
    19201 cctcgctacg ccagcaaggg ttcaccggtg agctgcatct gttttccgat gagcgacatc
    19261 ttccttatga acgacctcca ctctcgaaat ccatgttgct ggaagattcc ccgcagttac
    19321 agcaggtgtt acccgctaac tggtggcagg aaaacaatgt tcatctgcat tccggtgtaa
    19381 ccatcaaaac gctgggtcgc gacacacgag agttagtgtt aaccaacggc gaaagctggc
    19441 actgggatca gctttttata gcaaccggcg cggcagctcg accgctgccg ttgcttgatg
    19501 cactgggaga acgctgcttt accctacacc atgccggtga tgccgccaga ctgcgagaag
    19561 ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg aactattggt ctggaactgg
    19621 ctgccagcgc cacgcagcgc agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat
    19681 tgaactggcg gcaaccgtca tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt
    19741 acagcgccat cagcaggctg gtgtgcgcat tctgctcaat aatgccattg aacatgtggt
    19801 cgatggtgaa aaagtagaac tgacgctgca aagtggggaa acgcttcagg ctgatgtggt
    19861 gatttacggt attggtatca gcgccaacga gcaactggct cgcgaggcca accttgatac
    19921 tgccaatggc attgtcattg atgaggcttg ccgcacctgc gatcccgcga tctttgccgg
    19981 tggcgatgtg gcaatcactc gtcttgataa tggtgcacta caccgctgcg aaagctggga
    20041 aaacgccaat aaccaggcgc aaattgccgc tgccgcaatg ttagggctac cgctaccgct
    20101 actgccgccg ccgtggttct ggagcgatca gtacagtgat aacttacagt ttattggcga
    20161 tatgcgtggc gatgactggc tttgtcgtgg caacccggaa actcagaagg cgatttggtt
    20221 taatctgcaa aacggcgtgc ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg
    20281 cccaattcgc aaatggatcc agagcggcaa aacgtttgat gcgaaactgc tgatagatga
    20341 gaacatcgcg cttaaatcac tgtaatcagg ataattagcg aatatctcaa tgcctggggc
    20401 gtggcgaggt gcaagagtgt gtattacgtt taaatcacat tatcttgcaa agggattggt
    20461 tatgaacaca ctacgttatt ttgattttgg agctgcccgc cccgttttgt tattaattgc
    20521 ccgtatcgcc gtggtcttaa ttttcattat ttttggtttt cccaaaatga tgggctttga
    20581 cggtacggtc caatatatgg cctcgttggg cgcgccaatg ccgatgctgg cagcgattat
    20641 tgcggtagtt atggaagtgc ccgccgcgat attaatcgtg cttggctttt tcacccgtcc
    20701 gctggcggtg ctgtttattt tctacacgct gggtacggcg gtgattggtc accattactg
    20761 ggatatgacc ggcgatgcgg ttgggccaaa tatgattaat ttctggaaga atgtcagtat
    20821 cgctggcgcg ttcttgctat tggcaattac cggaccgggg gcaatttctc tcgatcggcg
    20881 ttaggaaaga tgccggatgc ggcgtgaacg ccttatccgg cattttataa attacagcca
    20941 ctctacccgc aacgacatct ctgaggttgt tgattcccct ggtgctagcg caatgagatc
    21001 acccccttcc gggcgatgat gctcatccgg cgcatggctc atcggttcca gacagaagaa
    21061 atcaaacgca tatcctttat caaacgcagg gtcggaaaca aagatgaaat aacacggtgc
    21121 aggtggcgtc gtttccatga tgatggcata accttcctgc ggctgctcga tgcgggcctg
    21181 accattccat ccggcaaaac cattgttcac ccactgacgc ggcaacggcg cgagctggtt
    21241 aaaatccagt tcctgcggta gctgctcgca aaactcaccc gccagccact gctcccgttc
    21301 cagccagtaa ccgctcgcct gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg
    21361 atgccagccg gtaccaaatg gcagcgtctc tgccccttga ttggtgacag agagcgtcac
    21421 cgtcagcgta tccgccgtta aatgaaacgc ctgacttacc cgatagtgat agacaccgct
    21481 gcgatgttca tacaccagac acaaactatc atcgctgtgc gagacacatt gccactcgcc
    21541 cagccagcca tcgccgtgta gatagtgcgc atcccactca acattcggtt gcagttgata
    21601 ctcacgcccc tgccagacaa aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg
    21661 aaaacatgag gcatcggttg ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt
    21721 atcgcgccag aagccttcga ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc
    21781 gtgggataag gtatagatgg tcatccgcta actccttaat ccgggaagtt aatcacaact
    21841 ttcccgcatt tgccgctcgc catcagcaca taggcatcac ctgcctgttc tagcgagaag
    21901 cgatgggtaa tggcgttacg cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt
    21961 tccatatgga acagactggt cacccaggag ccgataatcc gccgttgatg gtgcatcaga
    22021 tcagcgctga cctcgaattc cacttttccg gtttcaccaa tgtaaaccac ccgcccccag
    22081 tcagcagtgg attgcagtgc cagcaagcga cctgcggcat taccggaaca atcgagcgca
    22141 acatccgcgc caccgtgggt aagttcagca attatctgcg gcagaccttc ggtggttgct
    22201 aaatagccgt gatccatccc ccctaactgt tttgccatcg ccagacgttc cggcagcata
    22261 tcaacgccga tgatccgttt tgcaccgcga cctttcgcca gcatcatcgc catcatgccg
    22321 accggcccca gaccgaccac cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc
    22381 ccttcatagg ccgtgccaac gccgcaactg ataaacgcac catcttcgta gctcagcgca
    22441 tccggcagga ggatcagatc tttttcttcc gccagcagat attcggcatg accgccgtca
    22501 cgctgccagc cgtaagccgc ttttcctttg ccagtacaag agataggaaa gccgcggcgg
    22561 cagttcgggc aaaaaccaca gccagaaatg tgatacacca gcacgcggtc gccctcttta
    22621 aaatggcggc agccttgccc catcgccaca atctgcccgc acggttcatg accgttgata
    22681 aatccctggt ataacggttt atcgggtgcc gccgccgtgg cacggtgttg atgatagata
    22741 tagtggacat cgcttccgca aatcccggag gatttcattt tgatcagtac ctggttaatc
    22801 cccggcgtcg gcaccgcaac ttcccgcaga tcgacggttg aatttcctag taaataagca
    22861 gccagcatcg ttttcataaa tccctcatta acaatacgat taattttcat ccctgcagca
    22921 cccgcgccag gggcgttaag gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga
    22981 tattcgccag caccgccacc acgatgatga cgccgcgtac cacctgctgg aaaaaggagt
    23041 taataccgag cagcaccaga ccgttaccga ttagcgtaat caccagcaca ccaagcaatg
    23101 taccgaacag ggaaccgcga ccgccggaaa gtgccgtacc gccgaccacg accgcggcga
    23161 tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca
    23221 acaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt aaagataaga atgcgcactc
    23281 gacgaacgtt aataccgcac aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg
    23341 agcgcccgaa ggcggttttt cggctaatga acacaaacag cgcaaacaac accatcatga
    23401 tcagcgcgga tactggcaca ccgagaaatt gtccgcccag ccagtccagc acctcgtttt
    23461 cgtcaatcgg cactggcagt gcgttggtca taaacagccc cattccgcgc agagcgctcc
    23521 acagccccag tgtggcaacg aaacttggta cgttaaacac gccgcgcagc accccggcga
    23581 gcgttcccat cagcgcgcct aacagcaaca ccagcagaca agcaatcgcc aacggaactt
    23641 caaattgcag caaaaatgcc aggcacaccg agacaaaagc caccatcggc ccaacgctga
    23701 catcaatttc accggagata ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg
    23761 cggcatcgcg cagcacgttc atctggttat tcaatgagat aaagccaggc gcgttcaggg
    23821 agaagaccag ataaagaatg gcaatcacca ccagcaaacc gatctcatta atatggcgac
    23881 tgacaaattg tttgagcgag acgctcttgc cctgcggcaa tggtaatgac gaagcataca
    23941 tcttcaggtt cctcggtaaa attcatcagt gcacagacag aatggcggac atcagctcat
    24001 ccacattgac cggcgagtga aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc
    24061 ggtcacacac cagcggcaat tcctccactt cactggagat aaacaccacg ctttttcctt
    24121 cggcagccag ctcacggaca atacggtaaa tctgctgttt ggcttcgata tcgacgccgc
    24181 gcgttggctc gtcgagcaac aaaatctggc tggcggcata gacccaacga ccgatcacca
    24241 ctttttgctg attgccacca gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga
    24301 ccgtcatccg ctgcatcacc tcttcggtca ggcggcggat ggtggaccat tgcagcacac
    24361 cgttggtgct gattttttgc cgattggtca gcactgtatt ttcgtcaacg cctagccagg
    24421 gaatgatccc cgcttctttg cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt
    24481 cgccgtaatc ggggcgcatg attttctcgc cgttgataac aatttcgccc tgttcatacg
    24541 tctccagccc aacaatcgcc ttcagcaatt cactgcgccc tgcccccagc aggccagcaa
    24601 tgccaagcac ttcgccacga cgtagcgtaa agctgatatc ctccagcttg ggcttatggc
    24661 gtaacgcacg gacttccagt acggcctgat ccacaatttc ctgaggggca accggcgcaa
    24721 tatcaacgtg atcacgccct agcattagcg acacaatatg atgcgtggag gtgttttcga
    24781 gcatcacatc gcccgccacc tgaccatcgc gcataacggt ggcataggag gcaatgcggc
    24841 ggatttcttc catccggtgg ctgacataaa tcaccgccac gcccagtgcc gacatctttt
    24901 tcaccgcact gatcaccagt tcaacttctg cactggcaag cgagctggtg ggttcatcaa
    24961 gaatgaccac gcgcggctcg cccttcatca cccgtgcaat ttccaccagt tgcttttgcg
    25021 ccgggcttag cgttgaaaca agttgttcag gactaacgtc aacgcccagc gcctgtaagc
    25081 aacgttgggc atcctgcgcc atttgcaggt aatcaatcat gccgttgcgg cgaggccatt
    25141 gaccaaggca gaggttttct gccaccgtca gcccttccac cagacttaat tcctgataaa
    25201 ccgcacgaac ccccagttca gcggcacggc gagtcaacgt agcttcgtca ccttccagtc
    25261 gcgtctcgcc aatccagata tcaccgctat ccgggcgttc gctgccggta agcattcgaa
    25321 tgagagtcga tttgcccgcg ccgtttttac ctaacagcgc acgaacttcg cctttattga
    25381 gcgtgaagtt aacgttatcc agcgcaacga cgccggggta acgcttattt cctgccacca
    25441 cttttgctac cgggactgcc tctgttgccg tgaacatagg gacctctgcg aatcagcgat
    25501 tagggcagag cagaccatca acgtgcgttg ccagccactg tttaccatct tccgttttgg
    25561 tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta ttgataacct
    25621 tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc actaccgctt
    25681 tcagcacctg attgttttcc agctcctgag caatttcggt tgtcatatcc gaaccgaaaa
    25741 cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg agtgtcgcac
    25801 cgcccgactc ccccataatg gcgttgagat ccggcgtgga gataatcagt ttttcaccaa
    25861 cggaaatcgc tttatctaaa acagtccctt cctggttagc gacaatttgc gcgccgggaa
    25921 cgcgggattt taatacttct tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt
    25981 cgcaattgat gacggcaatt ttcggctggt caattttatt ggcaataaaa taatcggcgg
    26041 cagcgttacc cagtttttac caaattccag tggatcgccg accagatacg ccgagacata
    26101 tttatcgaca cccttttgat taatacaggt gttgtagcaa atcaccggaa tacccgcttc
    26161 actggcgcga cgaacggtac ggctactgcc attttcagac actgccgata aaataatggc
    26221 atcgacatta cgcgccacga gggtatcaac aaaggtactt tctttcgaaa tatccccctg
    26281 ggcgttagtt tcaattaact gtacctgtac tgaagaatct tttgccgcat cctgaacgcc
    26341 ctgacgcact ccagcgtaat atccctgggt atcaaggtat attgcaccaa tggtcatttc
    26401 tttatccgca gccctggcaa atagtgcgct acctaatagc gtagccatta ataatagatt
    26461 acgggttgtt ctcatttttt taggcatagt gcctcctgta gggtttttat taacaacggc
    26521 ttattctaat tattttgtga tgagcggcag cgcgtgccgc tcaaatatta cagttaatag
    26581 ggtttagtga acgtagttaa aaataaaagg catcacagtg gtgaataatg ccgctttcgg
    26641 ccaggccgga ttaatcgccg tcaattcagc gcgagcagac tcatattgcg caacctcccc
    26701 gagtcccgcg gacgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa
    26761 atcgccatac agcgataaca ggtcaggttg tgaaacagca aagaaatccg cctcaatact
    26821 ggttttcgcc atctcttgcg cccactgttt catttcacta aacagttgct gtgcggtttg
    26881 ctgttcaccc agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc
    26941 gttgtaataa ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg
    27001 catcgcttca gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc
    27061 gttatcagtt tgccccggta aacggccttc gcttaaattc tccggataat acagcgcggc
    27121 atgaagcagt tcgctggcct gctgcggctc tctggcatca agatgctgcc aggcgcgtaa
    27181 taactggttg aggataaact gactggtgac cttcccttcc ccgccttccc acgggtggaa
    27241 tttacgcgtg gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat gccacaaatt
    27301 gagcagttct gcggtcatgt cgtcgcgttt cagcgcaatt tccagattat tttccagacg
    27361 cgccagtcgt ttctccggtg tggctccact taacttatcc agcaaatccc gttcgaaaag
    27421 cagacgtgca tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc
    27481 ataatcgtgt ttcttattcc acgcatggat cgctaacccg cgccagccgt cggcaaactc
    27541 cggcgacatc tctacgcaac gttgccataa ggcaatggct ttgttgtagc tacgtttgtt
    27601 gtagtagaag caggccagta aatggcgagc aaaccaacac tcttcaatac tctccagcgc
    27661 cgccacttct tccagcgtat tcgggaaacg gacaaactgc gggaagacat caatggcttt
    27721 tgcgaccagt tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt aaagcggcag
    27781 cgtgcgctgg cagtccagtg cgttcagcat ctctgccgcc agggtgggca ttccccagtt
    27841 aatcagttgc ccggcggtca tcagagcgtt aacgtcgcgt ccctgacaca gaccgcgcca
    27901 ctgcgcgagg gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt
    27961 cagcggataa tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact
    28021 taacaccagc agcagattat gcaagcaaag cacttcctga ttggttgggc aggcacgaag
    28081 actttgttgg caaaaatcca gacccgcgtc gaagttatca ttacgcgccg ccagtcgtgc
    28141 cagaccataa tagccgcctg ctttactatt gccgctccag accgcacgcc agaaatcctc
    28201 ttcggcttgt tgatattgtc cctgacgttc gtaagcactg gcgcgaatca aacttgcttg
    28261 tccacactgc ggttttttgt tcagcgcatg tgcacgtttc agcgcctgac tggcatacgc
    28321 caccgcttgc gggaaatctg cgcggttata ttccagcatc gccagcgcca agttacagcg
    28381 ataatccagc gggtccagcg ccacgccgcg caggtagtaa tcgaacggtg aacggctggc
    28441 gtgatgatat tgctccagat gctgaccgat aaaccaggct tcatctgtac tggtaatgtc
    28501 ttgtgctgcc agtggcgctt tggcgacgtc cggcaacggc aactcctgcg gctgatgttc
    28561 ctgataactc agtacaatat tgccgtcggc atcggagagt tcaatggtca gtctgtcagg
    28621 attgataccg tgcaacacgc cctggatggc ggtcgctggt gtcagggcca cggcatcatc
    28681 gagcaacgcg ttgcatttgc cgatttcgcg gatcgccaga cggtatccgt tcaacggaga
    28741 gatggcatac agcccccact caatcccccg ctcactacgc tggagtttta tcaccgcatc
    28801 gcgggaggca ttttgcacca tgcccagaga atgataaggc aggaaatact gctcgaaacg
    28861 cttctcttcg taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa aaataccggt
    28921 catcagttcg atatacgggc cgttattgtc ggtcagactc ttatcccacg cctggccaaa
    28981 ttcactgtgt ccccaactcc actgtttttt acctggcgca atatggtggt tggcaacgtg
    29041 cagcaaaccg ccatcttcat cgtgacacca cgcgccgaca aaatcgtact gcgatttttc
    29101 ggccatatat gaggttggaa caggcacatt tttatagcga gaaatgtcca ctccggcgga
    29161 gtagtccact ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttgcc
    29221 gtgatcaaac accgcagtca catccggtgg gaaaacgctc tgatgccctt cacccccttt
    29281 cactgccggg ttgacccacc acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg
    29341 gctggcgatt tccagtgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa
    29401 accatgcatc ggctccgttt cgccgaccca caccgtctgt gcaccgtctt catgggcttc
    29461 gagggtgaaa tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc agttaaactc
    29521 aatcccgccg gagatccacg gtcccagcat ccccaccagc gcaggtttaa tgacttcatt
    29581 gtgatagaca aaatcgcgct gtttcacttt atcccatgcg cgatgcaccc gaccgcccag
    29641 ttccggcagg atcatcactt tgatgtagtc gttttccagc cacaccgcct gctaggattt
    29701 cagggttttc tgctcgctca gcgtatcggt cacgccgtag ggataaaccg cgccggacga
    29761 tccctgataa acgcgatttt ccaggaacat gggatgtata tcctgcggcc cggtttcata
    29821 ggtcgggatc tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt
    29881 aacaagacat actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt
    29941 cgcggaataa tcacgcaatt aactaaacaa ggtttagtga agatgagagc ctgcattaac
    30001 aatcaacaga ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag
    30061 cgcgccaata aatcaacgct ggcccggctg gcgcaaattt cgattccggc agtcagtaat
    30121 attttgcagg aactggaaag cgaaaaacgg gtggtgaata ttgacgatga aagccagacg
    30181 cgcgggcata gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgcctg
    30241 aacgtgacgc ccaccagtat tgagtgtcag gttgctaatg cttgtttaag tccgaaaggc
    30301 gaatttgagt atttacagat tgatgtaccg acgccgcagg cgctgctgtc cgaaatcgaa
    30361 aaatgctggc atcgccaccg taaattgtgg ccggaccgca ccatcaatct ggcgctggca
    30421 atccacgggc aggttgatcc ggtgaccggc gtatcgcaaa ccatgccgca agcgccgtgg
    30481 acaacgccag ttgaggtgaa gtatctgctg gaagagaagc tgggcattcg ggtgctggtc
    30541 gataatgact gcgtgatgct ggcgctggcg gagaaatggc aaaataattc gcaggaacgg
    30601 gatttctgcg tgatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa
    30661 atttatcgcg gcagtttgta tggtagcgga cagattggtc acaccatcgt taatccggat
    30721 ggagtcgtct gcgactgtgg acgttacggc agcctggaaa ccgtcgcctc gttaagtgca
    30781 ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg ttaatactca gcttgagcct
    30841 gaaaaactga caaccgcgca gttaatcgct gcctggcaga gtggagaacc gtggatcacc
    30901 agctgggttg atcgctctgc caatgccatt ggtttgagtc tgtataactt cctcaacatc
    30961 ctcaatatta atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt
    31021 aatactatta ttcgccagac aggatttaac ccgttcgacc gcgacgaagg accgagcgtg
    31081 aaagcgacgc aaattggctt cgggcaatta agccgcgcac aacaggtgct gggaattggc
    31141 tatttgtatg ttgaggcgca gttacgacag atttgatggc gcgataacgt agaaaggctt
    31201 cccgaaggaa gccttgatga tcataaacga aaaattgcct gatgcgctac gcttatcagg
    31261 cctacacgga gattgcaata tattgaattt gcaagatttt gtaggcctga gaaggtgttc
    31321 atgccgcatc caacatgaac aacgagcaca ttgtcagcaa atcaccgttt cgcttatgcg
    31381 taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct
    31441 tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttcggct
    31501 tctttaaagc cgcgacgggt aatcgccgga gtacctacac gaataccgga ggtcacaaac
    31561 gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga
    31621 gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg
    31681 ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct
    31741 ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc
    31801 agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa
    31861 acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccaa gatcaggccg
    31921 ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc
    31981 gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacaga
    32041 taagcaccga tgctgtcagc gatttcacgc attttcgccc agtcaaccac gccggaatat
    32101 gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg
    32161 gcgtaatcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg
    32221 gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga
    32281 acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag
    32341 tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt
    32401 tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca
    32461 gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt
    32521 tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc
    32581 cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga
    32641 ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag
    32701 tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc
    32761 ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc
    32821 aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca
    32881 tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa
    32941 aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca ccatcccttt
    33001 actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt ttactcataa
    33061 cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc aacgtgaagc
    33121 cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg cgctgctgcc
    33181 agcggtagaa aaaatcgcgc agaagcacac cagcttccag atcaaaccgg aacagtacaa
    33241 catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt
    33301 gctggacgcg tggggtaaag cctatggtgt actggctaat gtatttatca atcgcgaggc
    33361 ggaaatctat aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc gcgatttccg
    33421 cattgtggct aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg agccggtcga
    33481 cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc tgaagccgga
    33541 aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg
    33601 ctatcgtatc gcggtgaaac gcgaagaggg tgggcaggta tccaactggt tgcacaatca
    33661 cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct ttatggctgt
    33721 cgcagatgac acaccagtga cgttaatctc taccggtgtt ggtcaaacgc caatgctggc
    33781 aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt tccatgcggc
    33841 agaaaatggc gaagttcacg cctttgctga tgaagttaag gaactggggc agtcactgcc
    33901 gcgctttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca
    33961 gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca gcgatccgac
    34021 aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttaccgcga aacagttagt
    34081 ggatctgggc gtgaagcagg aaaacattca ttacgaatgc tttggcccgc ataaggtgct
    34141 gtaatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc
    34201 gtcgtcctct tcaccggtac ggatgcgaat gacccgagcc acgtcaaaga cgaagatttt
    34261 accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcgacac aggtatcgac
    34321 aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca ccatatactc
    34381 cgcgccgcgg tacagctcgg tatggccttt ctggcgacca aagcctttca cttcggtcac
    34441 cgtcatgccg gtaataccga cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg
    34501 ttttataatc gcatcaatct ttttcatgct attccttgaa aaggttgcct gtcttttgat
    34561 ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg
    34621 tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg ccgcgtgggt
    34681 gacgttgcct ttggtgattt gcagcagctt acgcaagtag ttgagttcaa aatgattacg
    34741 tgcctcaaca aaggttggca gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc
    34801 atcactaatc accggagatg aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg
    34861 gcgcacatta cccggccagc tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc
    34921 gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg
    34981 aatgtcttct gtgcgctccg ccagtgccgg aattttcagg ctgacaacgt tgaggcggta
    35041 atagagatct tcgcggaatt ccccgcgcgc catcgctttt gacagatcac ggtgagtggc
    35101 agaaataatc cgcacattga tatcaatatc gcggttactg cccagcgggc gcactttacg
    35161 ctcctgcaac acgcgcagca gtttgacctg taacggcgca ggcatatcgc caatttcatc
    35221 gagaaatagc gttccgcctt ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc
    35281 agtaaacgcg ccacgcgcat gaccaaacag ctccgactcc agcaattgct cgggcaatgc
    35341 gccacagtta atagcaataa atggtttgct gttgcgcggg ctggcgttgt ggatagcctg
    35401 ggcgaaaatc tctttcccgg tgccgctctg accgttaatc aaaacgctga cgtctgattg
    35461 cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg tgacaattgc
    35521 ctcgcgccag cgttcatcgg tggctggcgc ggattgctcc agcgcatcgt caattgcctg
    35581 atatagcgca tctttatcga caggcttggt gaggaaacta aaaacgccct gctgtgttgc
    35641 agcaacggca tcggggatag aaccatgcgc agtaagaata attactggca ttcccggctg
    35701 cactttctgg atttcagcaa atagctgcat accgtccatt tcatccatcc gcaggtcgct
    35761 gataactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcgc cactttccgc
    35821 cgtgaccaca ctgtagcctt cgctggtcag gcgcaggcca agcagtttca gcaatcccgg
    35881 atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg acctcatggg
    35941 tggatggcgc gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt ccagccggtt
    36001 tgcgggtcga gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt
    36061 ccagttgctg ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg
    36121 actgctggag tttactgtaa cgttggcgct cttccgccag ttgcagttgc agcgcctgac
    36181 cgtcgcgcca gagttgatac agtgggcgaa cctgtgccgg gatctcggta cttaacgctt
    36241 caatacgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa
    36301 tcccttgttt aaaggtattt tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg
    36361 actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa agcggattgg
    36421 tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa agatagtcag
    36481 ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc ttattgtgat
    36541 tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc cgtcggggca
    36601 ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc agtgggcgta
    36661 attgttgcag acagtctgga catggacagc gcggagaaac cggggcgtac atatcgtacg
    36721 tgaagatttc gagcactacc cggggccgaa ctgacaaata aaatagcctg ataggatagg
    36781 ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg
    36841 cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat
    36901 gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa
    36961 gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat tgatgacatc
    37021 aatataaacc cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg attcagcccc
    37081 gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc
    37141 caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag
    37201 gctatgagca gaaaccactg tctccaccag cggtgctaat tcaacattct ccagttccac
    37261 cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt
    37321 gcagctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac
    37381 aacctgatca gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc
    37441 atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact
    37501 taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccacaga acgaaacgct
    37561 attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg
    37621 cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag
    37681 cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg
    37741 agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc
    37801 aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt
    37861 gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc
    37921 atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc
    37981 gtccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc
    38041 ttcactacgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct gatcgctaag
    38101 cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggtagta gaattagcaa
    38161 aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt
    38221 gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca gatacaacaa
    38281 agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc
    38341 gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc
    38401 attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg
    38461 cttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata
    38521 cgttgctttg agggatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg
    38581 ctaaaaagag acaacttaag ataaacttat tagattacaa taaaatcaat catttaagtg
    38641 tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc
    38701 ccggagttgg gagcttatga tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc
    38761 aatgaactga tgacttaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg
    38821 ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc
    38881 gggtgcggca tcataatggt gactcgacca ctttcagtcg taaccgccgt aataccgttc
    38941 ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag
    39001 cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc
    39061 acgcgccctt caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac
    39121 agcagagacg ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag
    39181 gtgttacgca caaaacgtgg ccacaactca ctccccggga tcagttcacg caggttagac
    39241 atcatctggc aaccgttaca tacccccagg gccagcgttt gcggacgatg gaagaaggtt
    39301 gcaaactcat cgcgtacacg gtcattgaac aggatagact tcgcccaacc ttcaccggca
    39361 cccagcacat caccgtagga gaaacctccg cacgcgacca gggcgtgaaa atcctccagt
    39421 cccgtacgtc cagtcagcag gtcgctcata tgcacgtcga tagcatcaaa gcccccacgt
    39481 tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact
    39541 ttcggacgtg cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac
    39601 agttttacat tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc ctgatcggca
    39661 cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc
    39721 aacgtagtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca
    39781 ccggaaaccg cctgcccaac ataatggacg caatccgcaa gcccatgctg tgccagtacg
    39841 gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta
    39901 aacaacgccg ccaggcgatc gtcacccaga gaggcgatat ccgcgtcaat gccacaatga
    39961 cctgcaaagg ccatttccgc cagcgttacc agcaggcctc catcagagcg gtcgtgatac
    40021 gccagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgg
    40081 acaacatcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc
    40141 gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg
    40201 gtacaaagct gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc
    40261 accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc
    40321 atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca aagttcttca
    40381 cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc
    40441 atccagttgg cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg
    40501 gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc
    40561 gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc
    40621 gcgcagttag cgaccggcac ctgccacggc cccaccatct gatcgcgcgc caccatgccg
    40681 gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcagatgc
    40741 agtacgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc
    40801 gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc
    40861 ggcagatcga tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg
    40921 gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc
    40981 ggtaattgat cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt
    41041 tccagcgggc tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg
    41101 cgtccgccgt cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg
    41161 tggataaaca gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg
    41221 caacgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca
    41281 gactgaccag acgccatgga agacgccgcg ccgccgccaa gaccgatgtt cattgccggg
    41341 ccgccgagaa cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg
    41401 cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg
    41461 ccattgtggc tgttcacttt ttcttcataa gtacggaagt agccgttcag tgccggacga
    41521 ccaaattcgt tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg
    41581 gtaacaatgc gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt
    41641 cgcaggttgg atacggagaa accaaccaaa ccagctttcg gcttcgcgcc gcgcccggtg
    41701 gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc
    41761 gccgtcgggt ggttgtgagt ttcgactttc atcaggatgt gcgccggttc ctgatggaaa
    41821 ttgtagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg
    41881 gcggcgttat ctttataagc agagagaacg tgatctggcg tggtttcgaa agtatttttg
    41941 atcatcctga acagcgattt cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa
    42001 attttgtggc ggcagtgctc agagttcgcc tgagcgaaca tatacagctc gatgtcgttc
    42061 gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccaga
    42121 gccaagccaa gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc cagcaaatca
    42181 acgcttgtga ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc atctaaatca
    42241 aaaaagaccg tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca
    42301 ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt
    42361 tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca
    42421 ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccggacca
    42481 tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca
    42541 gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt
    42601 gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc
    42661 ataagtctct cgtcttctaa gctttcggtg tacccaaggg gggggaaacg ggcgtcatta
    42721 taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt
    42781 agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat
    42841 gccgctcatt tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca
    42901 cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc
    42961 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
    43021 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
    43081 tgacttataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
    43141 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
    43201 ttgacgacct tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg
    43261 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
    43321 ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg
    43381 ttgcaccggg tcatgtggta gttaacgatc tccagaccct gaaagaaaca aaattcccgg
    43441 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
    43501 aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc
    43561 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc
    43621 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
    43681 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
    43741 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
    43801 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
    43861 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
    43921 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
    43981 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
    44041 acgaacggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg
    44101 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
    44161 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
    44221 ggcatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt
    44281 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
    44341 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
    44401 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
    44461 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
    44521 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
    44581 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
    44641 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
    44701 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
    44761 aattttgcat caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat
    44821 catggcgacc aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata
    44881 ccgcgccgac cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac
    44941 gcatccaata ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
    45001 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
    45061 gcggggttac tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt
    45121 cgctatagcc actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct
    45181 tttcatgtcc cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
    45241 tggcgataag attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg
    45301 gctgcggaga gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
    45361 atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg
    45421 cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac
    45481 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca
    45541 acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc
    45601 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
    45661 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
    45721 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
    45781 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata
    45841 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
    45901 gtcagcgtta ccaagggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
    45961 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
    46021 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
    46081 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc
    46141 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaataccg
    46201 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
    46261 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
    46321 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcactg cgcgatatgc
    46381 atgcgctact cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct
    46441 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
    46501 aagtgctggc gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc
    46561 tgtataccat tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc
    46621 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
    46681 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
    46741 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact
    46801 aaaaaatgca tcaattgtga tatgtgtgaa atcaaataaa aacacattta ttttcatagt
    46861 gatacagcaa tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa attttgcata
    46921 agttcagcaa cacaaagaac accactttct ttaacgtcct ccgcgcccta aaggacgggg
    46981 ctttacggcg caaaaggcct gtgacataca acattaactt ttcagtcaac cgtagcacgt
    47041 tcttgtatac gccgtgtctg cggttttata taaccctggc tggaaacctc ttatacaaag
    47101 tcgattcgcc aacatcatag atgatcgcca ccttctggcg agaaactcct gatgcaatta
    47161 gtcgcccggc ctgcgcccat tattctggtg aaagtttggg acggcgaccg ccaattcgtc
    47221 cctgtgcgcg agcagcttcc agtcctgctt ttgttcgttc aacaatcagt tcacgttcca
    47281 tttcagccag ggcacccatc acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa
    47341 ttgaatcagt cagactacga aagttgatgc ctcgttcgcg caacgcctcc accagcacga
    47401 caagatgccg catactgcgc cccagccgat ccagcttcca gacaaccagt gtgtcgcctg
    47461 ccgataatgt cttgagcagt tttttcagcc ccggtctttc ggactttgta ccacttatct
    47521 tgtcttcaaa aatcagctcg catcctgcac agttcaacgc attacgttgt agatcggtat
    47581 tctggtcatt tgttgatacg cg
//
LOCUS       DAJTNP010000024        45688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTNP010000024 DAJTNP010000000
VERSION     DAJTNP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            114..2267
                     /locus_tag="OO160_001446"
     CDS             114..2267
                     /locus_tag="OO160_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5523625.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO160_001447"
     CDS             2320..4050
                     /locus_tag="OO160_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523626.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO160_001448"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO160_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5523627.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO160_001449"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO160_001449"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5523628.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO160_001450"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO160_001450"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5523629.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO160_001451"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO160_001451"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5523630.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO160_001452"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO160_001452"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5523631.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO160_001453"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO160_001453"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5523632.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO160_001454"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO160_001454"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5523633.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO160_001455"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO160_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5523634.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO160_001456"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO160_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5523635.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO160_001457"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO160_001457"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5523636.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO160_001458"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO160_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5523637.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO160_001459"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO160_001459"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5523638.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO160_001460"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO160_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5523639.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO160_001461"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO160_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5523640.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO160_001462"
     CDS             16308..16847
                     /locus_tag="OO160_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5523641.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO160_001463"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO160_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5523642.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO160_001464"
     CDS             complement(18206..18553)
                     /locus_tag="OO160_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5523643.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO160_001465"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO160_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5523644.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO160_001466"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO160_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5523645.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO160_001467"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO160_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5523646.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO160_001468"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO160_001468"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5523647.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO160_001469"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO160_001469"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5523648.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO160_001470"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO160_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO160_001471"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO160_001471"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5523649.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO160_001472"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO160_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5523650.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO160_001473"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO160_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5523651.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO160_001474"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO160_001474"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5523652.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO160_001475"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO160_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5523653.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO160_001476"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO160_001476"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5523654.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO160_001477"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO160_001477"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5523655.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO160_001478"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO160_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5523656.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO160_001479"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO160_001479"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5523657.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO160_001480"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO160_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5523658.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO160_001481"
     CDS             complement(40011..40466)
                     /locus_tag="OO160_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523659.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO160_001482"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO160_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO160_001483"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO160_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5523660.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO160_001484"
     CDS             complement(41309..41986)
                     /locus_tag="OO160_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5523661.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO160_001485"
     CDS             complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO160_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5523662.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO160_001486"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO160_001486"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5523663.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO160_001487"
     CDS             complement(43192..43650)
                     /locus_tag="OO160_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5523664.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO160_001488"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO160_001488"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5523665.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..>45688)
                     /gene="adrA"
                     /locus_tag="OO160_001489"
                     /gene_synonym="dgcC"
     CDS             complement(44596..>45688)
                     /gene="adrA"
                     /locus_tag="OO160_001489"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5523666.1"
                     /translation="ENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGLAGM
                     FLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAVLAG
                     MWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFN
                     SAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWET
                     MLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDV
                     IGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQM
                     SHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11872 a        11925 c        11181 g        10710 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atccactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttataat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgagcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gataccaggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaaaccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtt gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagacct ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcaacaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg tgcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactct ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttca
//
LOCUS       DAJTNP010000025        42614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTNP010000025 DAJTNP010000000
VERSION     DAJTNP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO160_001490"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO160_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5523667.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO160_001491"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO160_001491"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5523668.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO160_001492"
     CDS             complement(3736..4092)
                     /locus_tag="OO160_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5523669.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO160_001493"
     CDS             complement(4096..4512)
                     /locus_tag="OO160_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5523670.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO160_001494"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO160_001494"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5523671.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO160_001495"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO160_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO160_001496"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO160_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5523672.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO160_001497"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO160_001497"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5523673.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO160_001498"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO160_001498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5523674.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO160_001499"
     CDS             10521..11504
                     /locus_tag="OO160_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5523675.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO160_001500"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO160_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5523676.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO160_001501"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO160_001501"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5523677.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO160_001502"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO160_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO160_001503"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO160_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5523678.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO160_001504"
     CDS             complement(15306..15515)
                     /locus_tag="OO160_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5523679.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO160_001505"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO160_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5523680.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO160_001506"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO160_001506"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5523681.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO160_001507"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO160_001507"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5523682.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO160_001508"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO160_001508"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5523683.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO160_001509"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO160_001509"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5523684.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO160_001510"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO160_001510"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5523685.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO160_001511"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO160_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO160_001512"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO160_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5523686.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO160_001513"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO160_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5523687.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO160_001514"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO160_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5523688.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO160_001515"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO160_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5523689.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO160_001516"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO160_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5523690.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO160_001517"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO160_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5523691.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO160_001518"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO160_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5523692.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO160_001519"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO160_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5523693.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO160_001520"
     CDS             complement(34461..34604)
                     /locus_tag="OO160_001520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523694.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO160_001521"
     CDS             34605..34883
                     /locus_tag="OO160_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523695.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO160_001522"
     CDS             complement(34930..37026)
                     /locus_tag="OO160_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5523696.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO160_001523"
     CDS             complement(37026..37763)
                     /locus_tag="OO160_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5523697.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO160_001524"
     CDS             complement(37760..38398)
                     /locus_tag="OO160_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5523698.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO160_001525"
     CDS             complement(38512..38754)
                     /locus_tag="OO160_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523699.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO160_001526"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO160_001526"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5523700.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..>42614
                     /gene="lysC"
                     /locus_tag="OO160_001527"
     CDS             41391..>42614
                     /gene="lysC"
                     /locus_tag="OO160_001527"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5523701.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFG"
BASE COUNT        10602 a        10976 c        10458 g        10578 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgataccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggc
//
LOCUS       DAJTNP010000026        40536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTNP010000026 DAJTNP010000000
VERSION     DAJTNP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..946)
                     /locus_tag="OO160_001528"
     CDS             complement(<1..946)
                     /locus_tag="OO160_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5523702.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAG"
     gene            1192..1818
                     /gene="gstB"
                     /locus_tag="OO160_001529"
     CDS             1192..1818
                     /gene="gstB"
                     /locus_tag="OO160_001529"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5523703.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1815..2929)
                     /gene="yliI"
                     /locus_tag="OO160_001530"
                     /pseudo
     CDS             complement(1815..2929)
                     /gene="yliI"
                     /locus_tag="OO160_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3040..3423)
                     /gene="bssR"
                     /locus_tag="OO160_001531"
     CDS             complement(3040..3423)
                     /gene="bssR"
                     /locus_tag="OO160_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5523704.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3636..4961
                     /gene="rimO"
                     /locus_tag="OO160_001532"
     CDS             3636..4961
                     /gene="rimO"
                     /locus_tag="OO160_001532"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5523705.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5008..6336)
                     /locus_tag="OO160_001533"
     CDS             complement(5008..6336)
                     /locus_tag="OO160_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5523706.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6344..8686)
                     /locus_tag="OO160_001534"
                     /pseudo
     CDS             complement(6344..8686)
                     /locus_tag="OO160_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8864..9775)
                     /gene="gsiD"
                     /locus_tag="OO160_001535"
     CDS             complement(8864..9775)
                     /gene="gsiD"
                     /locus_tag="OO160_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5523707.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9778..10698)
                     /gene="gsiC"
                     /locus_tag="OO160_001536"
     CDS             complement(9778..10698)
                     /gene="gsiC"
                     /locus_tag="OO160_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5523708.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10716..12254)
                     /gene="gsiB"
                     /locus_tag="OO160_001537"
     CDS             complement(10716..12254)
                     /gene="gsiB"
                     /locus_tag="OO160_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5523709.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12274..14145)
                     /gene="gsiA"
                     /locus_tag="OO160_001538"
     CDS             complement(12274..14145)
                     /gene="gsiA"
                     /locus_tag="OO160_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5523710.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14132..15097)
                     /gene="iaaA"
                     /locus_tag="OO160_001539"
     CDS             complement(14132..15097)
                     /gene="iaaA"
                     /locus_tag="OO160_001539"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5523711.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15301..16536
                     /gene="moeA"
                     /locus_tag="OO160_001540"
     CDS             15301..16536
                     /gene="moeA"
                     /locus_tag="OO160_001540"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5523712.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16536..17285
                     /gene="moeB"
                     /locus_tag="OO160_001541"
     CDS             16536..17285
                     /gene="moeB"
                     /locus_tag="OO160_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5523713.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17464..18126)
                     /gene="fsa"
                     /locus_tag="OO160_001542"
     CDS             complement(17464..18126)
                     /gene="fsa"
                     /locus_tag="OO160_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5523714.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18257..19156
                     /locus_tag="OO160_001543"
     CDS             18257..19156
                     /locus_tag="OO160_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5523715.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19162..21594
                     /locus_tag="OO160_001544"
     CDS             19162..21594
                     /locus_tag="OO160_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5523716.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21740..22555
                     /gene="ybiV"
                     /locus_tag="OO160_001545"
     CDS             21740..22555
                     /gene="ybiV"
                     /locus_tag="OO160_001545"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5523717.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22707..23972
                     /locus_tag="OO160_001546"
     CDS             22707..23972
                     /locus_tag="OO160_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5523718.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24213..25805)
                     /locus_tag="OO160_001547"
     CDS             complement(24213..25805)
                     /locus_tag="OO160_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5523719.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26024..26944
                     /gene="ldtB"
                     /locus_tag="OO160_001548"
     CDS             26024..26944
                     /gene="ldtB"
                     /locus_tag="OO160_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5523720.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27002..28120)
                     /locus_tag="OO160_001549"
     CDS             complement(27002..28120)
                     /locus_tag="OO160_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5523721.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28117..28584)
                     /gene="mntR"
                     /locus_tag="OO160_001550"
     CDS             complement(28117..28584)
                     /gene="mntR"
                     /locus_tag="OO160_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5523722.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28770..28898
                     /gene="mntS"
                     /locus_tag="OO160_001551"
     CDS             28770..28898
                     /gene="mntS"
                     /locus_tag="OO160_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5523723.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29170..30753
                     /gene="opgE"
                     /locus_tag="OO160_001552"
     CDS             29170..30753
                     /gene="opgE"
                     /locus_tag="OO160_001552"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5523724.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30801..31316)
                     /gene="ompX"
                     /locus_tag="OO160_001553"
     CDS             complement(30801..31316)
                     /gene="ompX"
                     /locus_tag="OO160_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5523725.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31369..31434)
                     /locus_tag="OO160_001554"
     CDS             complement(31369..31434)
                     /locus_tag="OO160_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523726.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31669..32556
                     /gene="rhtA"
                     /locus_tag="OO160_001555"
     CDS             31669..32556
                     /gene="rhtA"
                     /locus_tag="OO160_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5523727.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32855..33358
                     /gene="dps"
                     /locus_tag="OO160_001556"
                     /gene_synonym="pexB"
     CDS             32855..33358
                     /gene="dps"
                     /locus_tag="OO160_001556"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5523728.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33762..34508
                     /gene="glnH"
                     /locus_tag="OO160_001557"
     CDS             33762..34508
                     /gene="glnH"
                     /locus_tag="OO160_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5523729.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34647..35306
                     /gene="glnP"
                     /locus_tag="OO160_001558"
     CDS             34647..35306
                     /gene="glnP"
                     /locus_tag="OO160_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5523730.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35303..36025
                     /gene="glnQ"
                     /locus_tag="OO160_001559"
     CDS             35303..36025
                     /gene="glnQ"
                     /locus_tag="OO160_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5523731.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36286..38510
                     /gene="ybiO"
                     /locus_tag="OO160_001560"
                     /pseudo
     CDS             36286..38510
                     /gene="ybiO"
                     /locus_tag="OO160_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38507..39433)
                     /gene="rlmF"
                     /locus_tag="OO160_001561"
     CDS             complement(38507..39433)
                     /gene="rlmF"
                     /locus_tag="OO160_001561"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5523732.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39709..39969
                     /locus_tag="OO160_001562"
     CDS             39709..39969
                     /locus_tag="OO160_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5523733.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40234..>40536
                     /locus_tag="OO160_001563"
     CDS             40234..>40536
                     /locus_tag="OO160_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5523734.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10095 a        10715 c         9865 g         9861 t
ORIGIN      
        1 cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga gtcacaaagg
       61 tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag gtcaataatt
      121 tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg gagattaaac
      181 gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc gtggttcctg
      241 tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg ttaggctgac
      301 gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt
      361 ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat
      421 ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg gcataaccat
      481 tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc gcaatacagg
      541 cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc
      601 ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat
      661 ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa
      721 ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca tccgcattgc
      781 cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc
      841 tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg aataaaaatg
      901 cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag
      961 tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt aataacgcac
     1021 acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca atatcctgcc
     1081 gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta actttacttc
     1141 accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag tatgattacg
     1201 ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct cgaagaactg
     1261 gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca cgatgctgat
     1321 tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga aagtgatctc
     1381 attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc
     1441 ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac
     1501 cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac accaccggaa
     1561 gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct gtttgccctg
     1621 ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat
     1681 atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg gacaccgcgt
     1741 ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg
     1801 atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact ggactcgtcg
     1861 gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg
     1921 tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca
     1981 tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat ttatcgctgt
     2041 tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca ggttgctcgg
     2101 tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag ttgattcccc
     2161 aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt tcatcaccac
     2221 cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc gccattcctt
     2281 gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc tttataaaag
     2341 gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc tgtaatttat
     2401 ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa agataacctt
     2461 taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt ggcatctggc
     2521 ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg ccataaccca
     2581 cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag atgcggcgag
     2641 actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg tgcgcccaaa
     2701 cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag tgacgcaact
     2761 cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc agtgcccagg
     2821 gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc gcccagagag
     2881 cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata acgcctccgg
     2941 attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt ttacaaaaac
     3001 ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct caagcatggc
     3061 gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt caagcttatc
     3121 ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac atactgcggc
     3181 tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat ccagatgaag
     3241 attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt tatctcgtag
     3301 atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca cgtatgcagc
     3361 gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc tgtcaacgaa
     3421 catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc tattttttga
     3481 gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac atttccttcg
     3541 cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta gaatccctgc
     3601 cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact ccccagccga
     3661 aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag cgtattctca
     3721 ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg aacatggtga
     3781 tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa gccattggtg
     3841 aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca aaagaagatc
     3901 agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc tatgagcagg
     3961 ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc ctgagcctgg
     4021 tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa atttcggaag
     4081 gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac ctgatgagcc
     4141 gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc gttaaagaga
     4201 ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat cgtactggct
     4261 tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag ttatcgaaac
     4321 tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac gacgtcatcc
     4381 cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag cacgccagcc
     4441 cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg gcgcgcatca
     4501 aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt gtcggcttcc
     4561 ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa gcacgtctgg
     4621 atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat gccctgcctg
     4681 accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag ctgcagcagc
     4741 agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg attatcgacg
     4801 aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa atcgacggcg
     4861 cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt gtgaaagtcg
     4921 agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag gccgggcaaa
     4981 atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc tgccgttttt
     5041 cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg tagtacattc
     5101 atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct ggaaatgaac
     5161 gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa tcaccgcaaa
     5221 ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg atttaaggat
     5281 tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt cgttaatgtt
     5341 tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca gcgcttttaa
     5401 atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga acgccgagtt
     5461 atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc catccagtac
     5521 gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat taatcactct
     5581 gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg caaagaaaat
     5641 aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt acaatccggt
     5701 cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt ttttagcatt
     5761 atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca ggttctcgct
     5821 gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca aaaatatcag
     5881 actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt caaaagagat
     5941 aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg cacaataatt
     6001 tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag gaccgacatc
     6061 gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa ccaacagctg
     6121 ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc gatattcagc
     6181 aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat acacccgttc
     6241 ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa aaatcagcag
     6301 actgactgta agtacgaact tattgattct ggacatacgt aaactactct tttactaatt
     6361 ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac ccctgagcct
     6421 gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg ccttccgcaa
     6481 tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt tttcccgcag
     6541 taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga ttaatttcat
     6601 aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc cgtacccctt
     6661 gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg atcacggttt
     6721 cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg atggtggtaa
     6781 aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata tgcagtaaga
     6841 aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt gccgttttaa
     6901 taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta atgaacttca
     6961 gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc gcgctcagaa
     7021 tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg ccaatttcca
     7081 ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg agtttcgcgt
     7141 catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc gccagtttca
     7201 gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg gcagacttat
     7261 ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc gctattttac
     7321 gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca ttgaatgtca
     7381 cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc ctgacgattt
     7441 tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac agtttgatga
     7501 tccccacagc cagatactga ttattgttgg actcaataat cgccatattt ttaataaaac
     7561 cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca atttcggcat
     7621 ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg ccttcgcgaa
     7681 ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta ttaaccagct
     7741 cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat accaccagca
     7801 acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg ttcaataaat
     7861 taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact gctgatgtct
     7921 ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg ctattataaa
     7981 taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct tgtttcaaaa
     8041 atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac cgccagttta
     8101 aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg atacgtagcg
     8161 taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga ttccagctgt
     8221 tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa tcggcaaaaa
     8281 taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga taaacaacga
     8341 ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt accagtccca
     8401 ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga atctcctctg
     8461 tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca atatctttaa
     8521 agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt tttaccgcgc
     8581 gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac agtgctgcca
     8641 ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta atacatttaa
     8701 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
     8761 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
     8821 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
     8881 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
     8941 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
     9001 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
     9061 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagaagaga
     9121 cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
     9181 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa
     9241 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
     9301 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
     9361 gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc
     9421 ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
     9481 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
     9541 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
     9601 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
     9661 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt
     9721 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
     9781 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
     9841 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
     9901 tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
     9961 agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
    10021 gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
    10081 acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc gcgccatcac
    10141 ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
    10201 tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa acatccccag
    10261 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
    10321 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
    10381 catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct catcggcaac
    10441 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
    10501 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
    10561 ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatgtggac
    10621 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt
    10681 gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
    10741 ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
    10801 agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc ctgcgccgcc
    10861 ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc caggaagtca
    10921 tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca gttttgcgag
    10981 gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca gccagtgtag
    11041 aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
    11101 gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
    11161 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
    11221 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
    11281 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg agttgcatag
    11341 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
    11401 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
    11461 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
    11521 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
    11581 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
    11641 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
    11701 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt cggtgaaatc
    11761 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta
    11821 atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag
    11881 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg
    11941 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt gtaagtaatg
    12001 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
    12061 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcattcgcg
    12121 tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg
    12181 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
    12241 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
    12301 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
    12361 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
    12421 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
    12481 cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga gatacatcac
    12541 cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa acagatacgc
    12601 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
    12661 atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
    12721 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
    12781 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc
    12841 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
    12901 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
    12961 tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc cgccctgcga
    13021 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
    13081 cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc
    13141 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
    13201 ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt cctgtttcgc
    13261 tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt tcatcgcacc
    13321 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
    13381 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
    13441 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
    13501 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
    13561 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
    13621 ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt gtgcctccgg
    13681 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
    13741 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg ggttcagcga
    13801 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc gcatttgtgc
    13861 agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca gcattttatc
    13921 gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg cagtcactga
    13981 cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca gactaaaaga
    14041 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat taagattttc
    14101 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
    14161 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
    14221 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
    14281 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
    14341 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
    14401 ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
    14461 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
    14521 ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
    14581 catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc gctgccagta
    14641 gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc atgccatgag
    14701 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
    14761 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
    14821 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
    14881 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
    14941 ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca acaatggcag
    15001 acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
    15061 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
    15121 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat
    15181 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
    15241 tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
    15301 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
    15361 gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg tcgtattctg
    15421 gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc tatggacggc
    15481 tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc
    15541 tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc
    15601 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
    15661 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
    15721 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag
    15781 ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
    15841 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
    15901 atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta
    15961 atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc
    16021 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
    16081 accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
    16141 ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
    16201 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
    16261 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
    16321 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
    16381 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
    16441 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga atgggtggaa
    16501 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga
    16561 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
    16621 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
    16681 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct
    16741 cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat
    16801 ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
    16861 tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta
    16921 cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc
    16981 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
    17041 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag
    17101 aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca atggaagcga
    17161 tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga
    17221 tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc
    17281 agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg catttgctcc
    17341 agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca gatttccaag
    17401 cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc
    17461 cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact tcgtcacagc
    17521 ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca gagtaattga
    17581 ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgctgccag
    17641 cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat
    17701 gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc
    17761 cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg tcggaatccc
    17821 ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa ctttcaccac
    17881 gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt cagcagtggt
    17941 agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa gttgcggaag
    18001 cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg tcacacccgc
    18061 cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat ccagatacag
    18121 ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc
    18181 tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa tgtttgaacg
    18241 aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg atggccccgg
    18301 tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc
    18361 ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc tggaaggctg
    18421 cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg gtttgcttat
    18481 tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct gtccgacaca
    18541 ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga ccgttctgcg
    18601 cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg gtgaaccctt
    18661 catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag gcattcatac
    18721 tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgccctatat
    18781 cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga
    18841 cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat
    18901 cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg taaaagccat
    18961 taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac
    19021 gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct
    19081 tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag ggttaaccgc
    19141 gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt
    19201 aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac cgagcgcgcg
    19261 cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg
    19321 ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga tgagttgatc
    19381 attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg
    19441 tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc
    19501 gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca gacggtacag
    19561 gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac cggcatcatt
    19621 aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctgctg
    19681 ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat
    19741 ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga tatcatgctg
    19801 gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat ggccgcgacc
    19861 gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg cgatcttatc
    19921 gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccagttg
    19981 attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga tcagtatctc
    20041 tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga acacgccatc
    20101 gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg ctccggctcc
    20161 cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg cggacaaaat
    20221 ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat cctcgaatcc
    20281 tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt
    20341 aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat gccggcattc
    20401 aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct
    20461 tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg gggctatcgc
    20521 tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg
    20581 cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggtaac
    20641 ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta ctacacgcgt
    20701 aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt gcacgatatt
    20761 ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc
    20821 gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg caacagcctg
    20881 gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc
    20941 gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac
    21001 ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc tcgcgcttat
    21061 cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg tggtccggtt
    21121 ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt tggcgcgcaa
    21181 actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg
    21241 gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg taaactgcct
    21301 acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc aacgctggag
    21361 aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga agtgcataaa
    21421 ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat
    21481 ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt cttcaccgcg
    21541 ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatctctt
    21601 cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt
    21661 cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt
    21721 tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg acggtacttt
    21781 tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc aggaactgaa
    21841 aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt
    21901 ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga
    21961 acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg
    22021 cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt
    22081 cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa
    22141 acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc
    22201 ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg gcattatgaa
    22261 acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa
    22321 cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg
    22381 cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa
    22441 tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg
    22501 cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca gctgacctct
    22561 ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat cacatcaaat
    22621 cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt agcatagttt
    22681 cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac acattgcctg
    22741 ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag cttggcgatg
    22801 tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct gaaatcaacg
    22861 cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat atcaaagcag
    22921 gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag
    22981 gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac tatctgaacc
    23041 gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg acgctcagcg
    23101 cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag gcccgccaga
    23161 gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg
    23221 gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc cgccgccttc
    23281 cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg ctggaacgct
    23341 ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat ctggcgaaat
    23401 acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg gacaacacca
    23461 ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat atgctgcctg
    23521 gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc
    23581 cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc
    23641 cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg
    23701 aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa
    23761 atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc
    23821 ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc
    23881 cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac ctcaacattc
    23941 acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat tgatgtggga
    24001 ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct taatgcctga
    24061 tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata ttacaggtac
    24121 tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc agccgtcgtc
    24181 accccgacag tatcggggtg acggataatc acttactcga tccctttact acgcaggtaa
    24241 tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag aatgcgggtc
    24301 gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt
    24361 tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt cggttcgtcc
    24421 atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg ccctttttcc
    24481 ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag caaacgaccg
    24541 agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact catccattcg
    24601 aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta accaatgcgc
    24661 gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac cagcgttttc
    24721 agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc accgacctcc
    24781 agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt cagaccttcc
    24841 acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa cgggttctga
    24901 cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt
    24961 gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg caactcagca
    25021 atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg ggtcgccgcc
    25081 gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc cagatccgcc
    25141 atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat catggtgctg
    25201 tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag gttgttggtc
    25261 ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag aagcacacgc
    25321 agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac tggaattccc
    25381 acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat
    25441 ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg caaagcatag
    25501 atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat caccgtatcc
    25561 agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat gcgctcgttg
    25621 ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat cttcataaag
    25681 gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg
    25741 acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt actggaaact
    25801 aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat aagtctgaaa
    25861 gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg
    25921 tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca atactctaat
    25981 attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata tgaaattgaa
    26041 aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac
    26101 ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga tcaccattcc
    26161 tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg ggctttccaa
    26221 tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta ctgtactgaa
    26281 cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc
    26341 agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg
    26401 cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg agcataagaa
    26461 agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga
    26521 accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt atgcactcta
    26581 catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg gcctgcgtgt
    26641 aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc
    26701 ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg agccagacgg
    26761 cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt ttgaaggtca
    26821 ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc agccagatgt
    26881 tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg ttcgcctgaa
    26941 ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta tttgccagac
    27001 cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc cacaacagca
    27061 tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc atacgtagcg
    27121 caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt accgcccatg
    27181 ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc acgttactga
    27241 tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa tgactcacgt
    27301 tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg ataaacatcg
    27361 ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc gccagcagca
    27421 caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc agcgccgtca
    27481 gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac tccggtgttt
    27541 gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac aacagcagga
    27601 gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg gcaaacgaaa
    27661 gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc agtagcgaac
    27721 cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc tcacacagtc
    27781 tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg gtcagaaagg
    27841 tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc gtagcaaagc
    27901 ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca cctttggtca
    27961 gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca ggccaggatt
    28021 tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat aactgaaaaa
    28081 aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt gtttctgggt
    28141 aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc cttccgcgtc
    28201 gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga ctatctgatg
    28261 tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca cgccacgcca
    28321 ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag ccaccgtcgg
    28381 ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca cttccctgat
    28441 caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg cctctctgac
    28501 ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt tttttgctat
    28561 tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat acatagcgtt
    28621 ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt aacgttttag
    28681 caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat ccctcaagga
    28741 tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt atgcgcgtgt
    28801 ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc gatatggtca
    28861 acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt acgccgcttc
    28921 actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact ttttgtcgga
    28981 tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg gcggctcgct
    29041 ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct ttcccctctt
    29101 cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg tttgttacga
    29161 tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg ggtatttagc
    29221 ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt aggttacccc
    29281 ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg gcgaacattg
    29341 cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg ttatttccct
    29401 tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca ctccaccaat
    29461 atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct ggtcggctta
    29521 tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga aaaagcgcgc
    29581 tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt tgttgctccc
    29641 gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta tccggtattt
    29701 cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca agaacggatg
    29761 gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc gaagtatcag
    29821 acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc ctttggcggt
    29881 cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc tgactacatt
    29941 gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt agtcgatggc
    30001 aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt ccagacctgg
    30061 tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag catcgccaaa
    30121 cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa aaacacgaaa
    30181 gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc gcaaccgcag
    30241 ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac acaaggcaaa
    30301 tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat gacgcaaacg
    30361 gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag cttctctctg
    30421 gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt gcaatacctt
    30481 gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat ttccagcggc
    30541 gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt aggctttttc
    30601 tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt tatatctgag
    30661 aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga ttacaaccat
    30721 ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc gagaggcgga
    30781 tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca ggtgcctacg
    30841 tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg
    30901 ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc
    30961 gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta gatgcttgcc
    31021 cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt tttgttgtag
    31081 tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat cacacccagc
    31141 gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt gttcatttgg
    31201 ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc tacggaagta
    31261 cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc
    31321 acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac
    31381 tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg
    31441 ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc
    31501 cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc
    31561 attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat tgggtatatc
    31621 cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat gcctggttca
    31681 ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat ggcgtctatt
    31741 cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc gggtgtcact
    31801 gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc atggcgactg
    31861 cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct gggtgggatg
    31921 aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt ggcgctggag
    31981 ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg
    32041 gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat
    32101 gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc tatttacatt
    32161 ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat tggttcgttg
    32221 attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc actctggcac
    32281 tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct gccttattcg
    32341 ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct gatgagcatg
    32401 gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata
    32461 cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc
    32521 aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt atggttatgc
    32581 ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata tcgaattatg
    32641 caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt gttcttatca
    32701 atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta ccactattag
    32761 tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta gtctcgttaa
    32821 ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat tagttaaatc
    32881 aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga aaaaagcaac
    32941 agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga ttaccaaaca
    33001 agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc tggatggctt
    33061 ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc agctgggcgg
    33121 tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa gttacccgct
    33181 ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg caatcgtcgc
    33241 taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag atatcctgac
    33301 cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca tcgaataaat
    33361 ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga caggaggccg
    33421 ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct atccccagct
    33481 gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag attgtttttt
    33541 catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg caccagattg
    33601 gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc acgtttttgc
    33661 cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat atgtgaatgt
    33721 cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct gtattaaaag
    33781 tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg gataaaaaat
    33841 tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc gataaatatg
    33901 tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg gattacgaac
    33961 tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac gtcgatctgg
    34021 cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc tctgacggct
    34081 actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg aaaagcgtga
    34141 aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt gattacgcga
    34201 aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac gcctatatgg
    34261 aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt ctgtacttca
    34321 tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa gcgcagcaat
    34381 acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac ggcgcgttga
    34441 aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc agtactgaac
    34501 cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc cctggggaga
    34561 ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga atttgattat
    34621 ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc tggcctgcca
    34681 ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc ggtctggctg
    34741 gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt tggatagcca
    34801 accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc gtccaggtga
    34861 tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac ccatttactg
    34921 cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg
    34981 cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt
    35041 gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg ccgccgctgg
    35101 gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc ggcgtggcgg
    35161 aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt gagatctgga
    35221 gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt ctgcgtcgtc
    35281 tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc actttggccc
    35341 aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg tggtgattat
    35401 cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac tggaagaaat
    35461 cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag ttgacgagcg
    35521 cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc cgcatctgac
    35581 ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca aagaagaggc
    35641 ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg cacatcacta
    35701 cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt
    35761 gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg aactgcgcca
    35821 tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg tgatcgtgac
    35881 ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg acaaaggccg
    35941 gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc agcgcttgca
    36001 ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac gcttatcagg
    36061 cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg ataaggcgtt
    36121 cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat atcccttcct
    36181 ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc ccctccccca
    36241 gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg gtggatcctg
    36301 ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca
    36361 accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa
    36421 aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt aaagagttga
    36481 tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg aagatcgtgc
    36541 cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc agccgccatt
    36601 atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc ggctccccgc
    36661 ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca atgttagcgg
    36721 tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta tatcgcaaaa
    36781 tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag cttccggcga
    36841 tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta tttgtcggcc
    36901 aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa caaagtttgt
    36961 ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg attttttgcc
    37021 caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt tactggggtc
    37081 gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc gtgccgatta
    37141 tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt atgctgtgca
    37201 tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc cagcatatgc
    37261 tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc tttgcgctgg
    37321 tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg ttgttcgatc
    37381 cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg attattggta
    37441 tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc accctctcgc
    37501 cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg ctttcggcgg
    37561 cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg ttgagcgcat
    37621 ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa accgtagata
    37681 tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg gtgctcgcca
    37741 gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc agcgcccgta
    37801 cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt accatcacca
    37861 tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca ggtgccgggg
    37921 cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt atcaccgggg
    37981 tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc gggccgttga
    38041 ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat accggggcgt
    38101 atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc ggtattggtt
    38161 cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc aatcaggcac
    38221 tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg attattggtg
    38281 aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg cgtgtttcgt
    38341 tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc caggtgaaaa
    38401 aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg ctgcctgctc
    38461 cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa cgttggcgat
    38521 taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag cgactctgct
    38581 tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg gtcagagcac
    38641 gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac atcacctgct
    38701 tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca ccgccttcac
    38761 accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc agccccaggt
    38821 tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg aatggcgggt
    38881 tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa atcgccccac
    38941 tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca ctgataatcg
    39001 cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc cagccatatt
    39061 catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc agaatgctgg
    39121 cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga tgaatataat
    39181 ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag ttcgctacgg
    39241 cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga ttggcaaagt
    39301 ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca ggattgacct
    39361 gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc aaccccggtt
    39421 tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca taacgcgaca
    39481 gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta tggtttatgg
    39541 caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt tgtaaaaata
    39601 tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca tcgcgcaggc
    39661 gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat gaaaaagtgc
    39721 ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta tgccgcgcaa
    39781 ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc ggcaactggc
    39841 gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga acaaggcgcg
    39901 aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac tgcaaccatc
    39961 tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc gcctgttttg
    40021 taatcaaaaa acatcaaatg tgaacgcaat gtaaataaat gtatttcttt ttcgcgcaat
    40081 gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca cttgcacgaa
    40141 cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg tggcagtgaa
    40201 aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa tttccctgcc
    40261 agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac gcctgtggca
    40321 caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt cgaagcatca
    40381 acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg tccggtagcg
    40441 gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca gggcgcaacc
    40501 aaccttaccg acgcgctcaa aaacgtcccc ggcgtg
//
LOCUS       DAJTNP010000027        38787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTNP010000027 DAJTNP010000000
VERSION     DAJTNP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO160_001564"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO160_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5523735.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="OO160_001565"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="OO160_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5523736.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="OO160_001566"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="OO160_001566"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5523737.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="OO160_001567"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="OO160_001567"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5523738.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="OO160_001568"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="OO160_001568"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5523739.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="OO160_001569"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="OO160_001569"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5523740.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="OO160_001570"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="OO160_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5523741.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="OO160_001571"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="OO160_001571"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5523742.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="OO160_001572"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="OO160_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5523743.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="OO160_001573"
     CDS             7139..7750
                     /locus_tag="OO160_001573"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5523744.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO160_001574"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO160_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5523745.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO160_001575"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO160_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO160_001576"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO160_001576"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5523746.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="OO160_001577"
     CDS             complement(11944..12303)
                     /locus_tag="OO160_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523747.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12953..13516)
                     /locus_tag="OO160_001578"
     CDS             complement(12953..13516)
                     /locus_tag="OO160_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523748.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13723..14661)
                     /locus_tag="OO160_001579"
                     /pseudo
     CDS             complement(13723..14661)
                     /locus_tag="OO160_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14724..15098)
                     /locus_tag="OO160_001580"
     CDS             complement(14724..15098)
                     /locus_tag="OO160_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5523749.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO160_001581"
     CDS             complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO160_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5523750.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO160_001582"
     CDS             complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO160_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5523751.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17168..18058)
                     /locus_tag="OO160_001583"
     CDS             complement(17168..18058)
                     /locus_tag="OO160_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5523752.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO160_001584"
     CDS             complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO160_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5523753.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO160_001585"
     CDS             complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO160_001585"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5523754.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO160_001586"
     CDS             complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO160_001586"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5523755.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO160_001587"
     CDS             complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO160_001587"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5523756.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO160_001588"
     CDS             complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO160_001588"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5523757.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO160_001589"
     CDS             complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO160_001589"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5523758.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO160_001590"
     CDS             complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO160_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5523759.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO160_001591"
     CDS             complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO160_001591"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5523760.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO160_001592"
     CDS             complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO160_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5523761.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO160_001593"
     CDS             complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO160_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5523762.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO160_001594"
     CDS             complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO160_001594"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5523763.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO160_001595"
                     /gene_synonym="manB"
     CDS             complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO160_001595"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5523764.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO160_001596"
     CDS             complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO160_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5523765.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="OO160_001597"
     CDS             complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="OO160_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5523766.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO160_001598"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO160_001598"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5523767.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO160_001599"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO160_001599"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5523768.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO160_001600"
     CDS             complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO160_001600"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5523769.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="OO160_001601"
     CDS             complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="OO160_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5523770.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38666..>38787)
                     /locus_tag="OO160_001602"
     CDS             complement(38666..>38787)
                     /locus_tag="OO160_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5523771.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10354 a         9714 c         8730 g         9989 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa
    12541 tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac
    12601 atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg
    12661 tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa
    12721 tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca
    12781 ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt
    12841 tttcgctatc aatataacaa atatgatggc tccatatatg gcaccaaata gttttacgcc
    12901 acaagcaatt ccaataaaaa aaccacataa aaaaattaat tcattttgtg ttttattgtt
    12961 ttctgagcga atatatataa gaatactggc tgcagcaaaa caaagatagc gttaaatcaa
    13021 tatatgcata accaagatga tctttaagtg gattgatagt aaatagtagg attgtagcaa
    13081 taatgcctgg ataataagtt ttcgtaattt cccatgaaca gccaacaagt ccacaggcta
    13141 ttacaaaaaa acagatattt gctaaaaact gagctaacct tacatctcct aactgtaaac
    13201 ctaaagccat taaaagatct gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa
    13261 caacaattgc atggtgctca actaatgaac gagccaaagg taagtgatac atagtatcat
    13321 cccaatcacc gggagctcgc agggacgata acaatgtaac cacaaaaatc aaaacaagaa
    13381 ataaaataac aaacagcgta ctgaactcaa tattatttat tgccagccat ctaaattgct
    13441 gacgtttaat aatgcttaaa acccaacaca ccgtaaaaat taaaaagata gcaatcttat
    13501 taaattgccc gaccatgccc agaaagtaaa ttatcaaaat atttaaagac atccctatgc
    13561 ataggcaata aaaaataaag aaaatttgtt aagtcctgcc accccctcct taaaaaaaga
    13621 tgacaataca atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac
    13681 atacaaatac aacggcaatt caatcatagt aaaatactct atttacatta tcagtttata
    13741 gtttttttca gaaagataaa ataatgcctt catttcacgt ctagttgttg tatttgaatg
    13801 caaaataatg ccacatacaa atgccattat tgacgataac atgatggagg cagttaaaaa
    13861 ggcagtcgga aatcttctca ccaaacctgt atcaatataa tcaacaatga tgggcaatcc
    13921 taaaattata gacaaagcag caagtaaaac gccgataata ctaaaaaagt atagtggccg
    13981 ttcaagcgag taaagtttaa ttattgtttt cagaatcttg aacccatcac tccaagttga
    14041 tagtttgctc acactccctt cagatcgctc gccatatttt gtattaacct caccatactt
    14101 catacgtaat tctaaagcat gaattgtaag ctcagtttcg atttcaaatc cccttgatag
    14161 acatggaaaa gatttaacat agcgtctgga aaatacacga taccccgaca acatatcagt
    14221 aaaattccct ttaaagatag tctgaacagt accagtcaac aatttatttc ctcagcgatg
    14281 cccttttctg tatgtatttt gatctccatt ctcttgacga catcctacaa ccatatccaa
    14341 ttgatttttt atgagatggt taatcatttc tggagcagaa cttgcatcat aagtgtcatc
    14401 tccatcaacc attagataaa tatccgcatc tacatctgag aacatacgtc ttacgacatt
    14461 ccccttgcct ttcagaggaa cagagtgaac tgttgctcca gcatcctctg ccacttgtgc
    14521 tgtactgtca gtcgaattat tatcaaatac atatatagaa gcagttggaa tattcgtttt
    14581 gaaatcattt atgacttttg ctattgcctt ctgctcatta taacaaggaa taattacagc
    14641 gatattaaaa gagtgataca taagcccctc atataaacta tttagctaat gtgcaagtaa
    14701 ttgtgttttt aacaaatcgt ttactaacga aaaatgaaat attttgatgc gcaatatcct
    14761 aaaaccaaac taaaaagaga aaaggtgaac aatgtaaata ttggagataa agctaagaga
    14821 tcaaataaaa cgccgaataa ataactcatc gcccccataa aaaatataaa tataaaatat
    14881 ctagccttgg ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt
    14941 gctgcacata taaaaccaac aatgttactt cggccttgac caaaagctaa attattataa
    15001 cacgcataaa atacccccca atgtaaagca gtattgacaa ttccaattaa aaaataacta
    15061 aaaaaagaac tggtcaataa cttccctatt tttaacatcc tttattttgc tccagaagtg
    15121 aggttattac taatttggat attttctata gaaaataccc ataaaactac aacgcctata
    15181 ataaaaattc ccatgggtgt aaaaagaaat ggattagtta tagaaacggt aatactgaaa
    15241 aaaagtatta acgacatcat taatgatgtt tttgtactat ttaaaaaata gcctttcaaa
    15301 aagataagca acataggaac taatgatata aatacaccaa taacgcctgt tttctgaagt
    15361 atctcaagag gtactatttc aatccttaat cgatctaata taaatgaacc aaatcctctt
    15421 ccaaacaaga acgtcgctaa atcaacattt ttataataaa aatataaatc attaaatcta
    15481 acagagtcag aatcttcact gcgtgatccg accatgtaaa gcataattat aaatagaacg
    15541 ctgcccaata ttattataat agtatttaaa tatagcttat gtaatttaaa actggctaat
    15601 aacagtgata atgttgtaaa aagtaataca cctcttgatt ctgtcaataa tatcgcaagc
    15661 actattatga gacattttaa atattttttg tcgacaaaac tgataatcgc accgacacca
    15721 aaaaagtaga aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc
    15781 aatgataagt gttcataaat taaattaaaa tttaacaaac cgaaattcaa caaaattatg
    15841 tatgaaatat ataacagtga tccatataat gcaaatgcat taactatttt aataaaaatc
    15901 tcacatgaaa atacttttaa agtatatctt agtgtaaata gatatataag tgacagcata
    15961 aaaaggtatg gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg
    16021 acaaagaagc cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg
    16081 gtgattgaaa aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat
    16141 aataaatatc gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata
    16201 atcagttcaa tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg
    16261 tgatgttttt taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt
    16321 tttccgcctt aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc
    16381 caattgatta ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt
    16441 tcgataccac ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt
    16501 tgctcccact tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata
    16561 ccacgaataa tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc
    16621 ttgtgcagca tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt
    16681 tataacatat gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga
    16741 ctgagcctta tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga
    16801 ataacaattc gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata
    16861 ccaaatatcg tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt
    16921 aattaatcta taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt
    16981 tataattgag ggatatgata attgaaattc ttcacaaata tttaatgtat tatcagttga
    17041 taaatcatct gatataaaaa ttgagatgtc tacatctttt tgttcaataa tactttttaa
    17101 ttgttcctca atccagcatt cgccattata cgtagccata agtacagcta ctcttttttg
    17161 atcagaacta tgcattataa cgaccgcccc cagtaattcc tcttattcca tccagcgtag
    17221 ccaaaatatg ttttttcata tattcaattt tatttttttg agttgcgata attaaagtcg
    17281 tatgaattaa agaaaaaaca acttccctaa tagagagtaa aagaggcaca tgattttttc
    17341 taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag tgaattggta
    17401 tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt tctatatcag
    17461 gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct gtatcgacat
    17521 aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct tttgaaacca
    17581 tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca gtgacatgaa
    17641 ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt gacttatctc
    17701 tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag caggaaagtt
    17761 ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc ttctctgccc
    17821 ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct tcattcctac
    17881 caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag gtgaaattag
    17941 tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg atcagtgcat
    18001 tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta ctattcatta
    18061 tccaagtgac tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata
    18121 aagccattgt aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc
    18181 cagtaaccca tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc
    18241 acaatgaatc aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat
    18301 cattgccagc gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt
    18361 aatttgataa ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat
    18421 gattatcacc ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt
    18481 aaattcttta aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc
    18541 aggtattatt cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc
    18601 tgcccccata atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat
    18661 attacttact gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa
    18721 atgaaccccc gaaattttaa tatcattcct gacataatag gcagagacta aaatcgaaat
    18781 aactcttgca aatattaaac cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa
    18841 tatggccgga attcccgcga tgcaagagct tgatatagat ttttgaatat ttacaatgcc
    18901 aaatttttca tccccctcaa gaatggctga ccataattga tttagaataa ataatggaat
    18961 gcaaatagcc aacagtttaa atgctagttg tatatcacta tgttcaacac cagaaatttt
    19021 tagataatta acaattccat cagagaaaat caataataaa aaagcaccaa agcatgaaaa
    19081 tagcaccaaa aaagatgtac ttgttgaaat taccttggtt ctttcatgat gattatcacg
    19141 atgaattgca atttctctaa taacagagcg agtcaggcct acatcaaaaa ttccagcata
    19201 cccaacaagt gcaattgcaa ttgtataaac accgaaacct tcaggcccca aacctcgagc
    19261 gagaaatcct agcgagggga tagcaattaa agttggtatt gcatagccaa aaaggttcca
    19321 gacactattt tttattatgc tcattattac atttctttat catataaaat aatcagcatc
    19381 tacaaatttt tgaccattaa tatctttatc agaaagcaat ggattttgaa ccggccattc
    19441 tatatttata cttttgtcgc tccataaaat cccacgctct tgcatttttg aatagaaatt
    19501 atttgttttg tagacaaatt ctgcttcatc actaagaacg caaaacccat gagcgaatcc
    19561 ttccggtatc cataattgac gcttattctc cgccgacaaa tttacaccaa cccacttgcc
    19621 aaatgttggt gatgatttcc gaatatcaac agctacgtca aaaacttcac cgacaacaca
    19681 gcgtaccagt tttccttgag cataaggttc cagctgatag tgcagtccgc gtaacacacc
    19741 cttagttgat ttagaatggt tatcctgaac aaactcaacc tttcgtccta cagcctcttc
    19801 gaaaactttc tgattgaagc tttccataaa aaagccccgc tcatcaccaa atacttttgg
    19861 ttcaaaaatt agcacatcag gaatttcagt tttaattacg ttcattttat taataacctt
    19921 taatcatttt cagcagatac tgaccataag catttttctt cagcggctcg gctaatacct
    19981 tcacttgctc agcatcaata aagcctttac ggtgagcaat ttcttccggg caggaaactt
    20041 tcagcccctg acgctcttca attgttgcaa tgaagttgct tgcttcaata agactttgat
    20101 gtgtccccgt gtccagccat gcataaccac gccccatcat agcgacagac aaacgtcctt
    20161 ggtccatata aatacggtta atatcggtaa tttccagttc gccacgtgca gatggtttaa
    20221 ggtttttcgc catttctaca acgtcattgt catagaaata aagcccagta accgcatagt
    20281 tactttttgg ttcctgcggt ttttcttcca gactaattgc cgtaccgtta tcatcaaatt
    20341 ccaccacacc gtagcgctca ggatcattta cgtgataggc aaaaaccgtt gcgccactct
    20401 ctctgttaac agcggtatcc attaatttcg gcaggtcgtg accatagaag atattatcac
    20461 caagtaccag cgcacaatca tcaccaccta taaattcttc accaatgata aatgcttgcg
    20521 ccagaccatc cggactcggt tgtactttat attgcagatt aagcccccac tggcttccat
    20581 caccaagcaa ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc agaatatctc
    20641 gaatacccgc caacataagc gttgaaagcg gataataaat catcggttta tcataaatcg
    20701 gtagcagttg tttgctcaca gccattgtca caggataaag acgagtgccg gaaccaccag
    20761 ccagaataat acctttacgc gttttcattt caccattcct tttaattcat ctcgctctag
    20821 catcatgagc gagatgcaat aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc
    20881 atacgtttca cgcccacctg ccagtcaggc aagacaagcg caaagttctg ctgaaatttt
    20941 tcggtattga ggcgagaatt atggggacga cgtgctggtg tgggataggc cgttgtcggc
    21001 acggcattaa gtttgttaag agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc
    21061 agagcggcat aatcgtgcca ggttgttgta ccaccagcaa ccagatggta caaaccagca
    21121 acttctggtt tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca
    21181 gcccccgttg gtgcgccgaa ttgatcgttt attacagcca actcttcgcg ttcttttgcc
    21241 aggcgcaaca tcgttttggc aaagttattt cctttaccag cgtataccca gctggtacgg
    21301 aaaataagat gctttgcgca atgttcttgt aacgcttttt ccccagctag cttggtttca
    21361 ccgtagacat ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc accgttgcct
    21421 gggaaaacat aatcagtgga gtagtgtata acccaggctc cgacttcatt agctgctttt
    21481 gcaatcgctt cgacgcatgt cgcgttaagt aattgcgcga aattcggttc tgattccgct
    21541 ttgtctactg cagtgtgagc agcagcatta acaataacat cggggcgaat ttttttgacg
    21601 gtttcagcca caccttcggg gttgctgaaa tctccacaat aatcagtgga gtgaacatcg
    21661 agcgcaatca agttacccag aggagccaga gcacgctgca gttcccaacc cacctgccct
    21721 gttttgccga aaagcaggat attcattact gacggccctc atagttttgt tcaatccacg
    21781 actgataagt accgcttttc acattctcaa cccaatttgt attagccaga taccattcca
    21841 ccgttttacg aatcccgctc tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa
    21901 ttttatcggc atcaatggcg taacggcggt catgccccgg gcgatcggca acataagtaa
    21961 tttgctcacg ataagatttc tctttcggta caatctcatc caacaaatca caaatagtga
    22021 gcactacgtc gatgtttttc ttttcgttat gtccaccaat gttataagtt tcacccgctt
    22081 taccttcggt tacgacggta tataacgcac gagcatgatc ctctacatac aaccagtcgc
    22141 gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca ttcagaataa
    22201 ccaatggaat aagtttttcc gggaagtgat aaggaccata attgttcgag caattagtca
    22261 caatggttgg taaaccataa gtccgcttcc aggcacgtac caaatgatcg ctggaagctt
    22321 tagacgcaga atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg
    22381 cttcgttatt atttgcttca tccggatggg gtaagtcacc ataaacttca tcagtagaaa
    22441 tatgatgaaa gcggaaactt tttttctttt catcattcag agcagaccag taattgcgcg
    22501 ccgcttctaa aagaacataa gtccccacaa tattggtttc aataaatgcc gcagggcccg
    22561 ttattgaacg gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct ggctggtgct
    22621 gtgcgaaaat acgcgacatg gctacagcat cgcagatatc tgcatgctca aaagcataac
    22681 gttcagaatc agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga
    22741 cattaacaac actatcttgc gtattattta ttatgtgacg aacaacagca gaaccaataa
    22801 accctgcgcc accagtaaca agtatcttca cttttttatt ccatatagct atagagcatg
    22861 ctgtgaaata gactgctcca gatttgatta atagaggtat taatacacgc taccgcccct
    22921 ggcttaacag ctaccagtgc actgcatgca tctcaacgat gtggcgagcg ttagccactc
    22981 gcgccaaatc cgaaaaattc aaacgttaat tgtcttacca aacagccgta gaaacaagga
    23041 aaatcctgga aaaatttgaa taatatgagc tatgtaactt cttgttttta taggctttat
    23101 aaaaacggca acggcaggaa aattcaatga gcaaaactta tgccacgaat cttcactgcc
    23161 gttataattt tcttatcaac cgttacatcc ggtcagattt tcattattcg cttaacagct
    23221 tctcaatacc tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa
    23281 acgcctgcat atagcccatt tttttgccgc agtcgtaact gtcgccggtc atcagcattg
    23341 catcaacgga ttgttttttc gccagctcgg caatagcatc agtcagctga atacgtcccc
    23401 atgcaccagg ctgagtacgt tccagttccg gccaaatatc ggcagaaagc acatagcgac
    23461 ctacggccat gatgtctgag tccagcgtct gcggctgatc cggtttttcg ataaattcaa
    23521 caatgcggct gactttaccc tcacggtcca gcggctcttt agtctggatg acggagtatt
    23581 cagagaggtc acccggcata cgttttgcca gcacctggct gcggcccgtt tcgttgaaac
    23641 gtgcaatcat ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa
    23701 cgtctggcag taccacgaca aatgggttgt caccaatggc aggtcgcgca cacaaaatgg
    23761 agtggcctaa acctaaaggt tcgccctgac gcacgttcat aatgatcacg cccggcggac
    23821 agatggactg tacttccgcc agcagttgac gcttcacgcg ctgctcaagg agtgattcta
    23881 actcataaga ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga gttaccagga
    23941 ggatttcttt gatccctgca gccacaatct cgtcaacaat gtactgaatc attggcttgt
    24001 cgacgattgg tagcatctct ttgggtatcg ccttagtggc aggcaacata tgcatcccga
    24061 gacccgctac aggaataact gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg
    24121 ttgctgaatt atagcttgtt cgattttttt cgccagcatc aattaccctg aattgattac
    24181 tgaattactt gtgatgttac gccgcttcgt tgtggattgc agtagcattg ttcctaagta
    24241 tggctccatt tttccaggaa tggtcgcaaa tctactccct ccgttccggc aatctaaagt
    24301 taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg gagctttgcc
    24361 ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt ttcgccataa
    24421 agacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgctggt ccaactgagg
    24481 attcctgcat cacattgata tttcgcataa aaagatgttg cggcttgttg tgtaactcca
    24541 gcgaggcacg cttcatctca atgttagtta gcgacacaaa ggagacggca ttcccggcgg
    24601 agatttggat gccgcgcaat ttataagcaa gatgggtatt atccagttga atattattca
    24661 ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag ccgattaaca
    24721 tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa ccgtaaatag
    24781 cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata ttgcgggcat
    24841 tgatattacg aataacaaaa tgtttaccat tttcaacatg gatcaactgc cgacaatccg
    24901 atcccgtgat attcgccacg acaaagtttt tcactgcctg gtcttccggg tagttgttat
    24961 cataagtgct tcccgcaagg cctatgccga tgccccagtt gattttgccg ttggtacagt
    25021 tgatacgctc gatgacatgg tcagatatca aaatatcact gtcgttaatt gccacgttcc
    25081 attcaatggc gtcgccctgt aagtcgctga acttacagtt ggtaatgttg gcaccgataa
    25141 tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctgaca gtgaggttat
    25201 cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc aatataaatc tgcattaccg
    25261 ggccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc acatccaggg
    25321 taatgttatg cataccgccg ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa
    25381 atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg ccgggaggga
    25441 tgaagatgcc ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc
    25501 cttcgctgaa ggcttgttta aatgaggcga tccagtcatg tgggttgtag tcgttaatgt
    25561 taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg
    25621 agcttgccgt caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt tctcctctat
    25681 aaagcctgca gcaagctggc gagttctcga ttaatcacct gctggttaaa atcgtgttcg
    25741 actttttcgc acgcacgttt gacgaccgga gccagttcgt cggtatccag ttggctaaac
    25801 gccgccagac gttgtgccag tgcgcgagca tcgttctcag gcagcagcca gccggattta
    25861 tcggcctcca ccagttcagg aattccgcta tgcagagtag aaaccaccgg aataccgacc
    25921 gccatcgctt ccattagcgc caccggaatg ccttccatat cgccatccgc gcccgttacc
    25981 gatggcaaca ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg gctcggttta
    26041 aagcccggca tctctaccac atcttccagt tgatattgtt cgatgatggt gcgcaggcgt
    26101 cgttcccaca ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg ctctttcaac
    26161 tgacggcagg cttcaatcgc cacatgcagg ccttttttct cggttaagcg tgcgacagat
    26221 atgatttcca gcggcgttgc gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc
    26281 acgcccatac gcgatacggc gattttttct ctggggcagc ccattttttg cagccttccg
    26341 gcccacagat cgcttatcgg taacatcagg tcgccacggc gaaacagttg ttgatattcg
    26401 ggagtgtagt ggttgagcac ttcccgactg gagatatcaa taccgtggaa gatggtggca
    26461 attttgccgc gaatgatacc cagttcgcgt agttttgctg cggttacccc cgccgggcca
    26521 aaatgagcga tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac
    26581 aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt
    26641 ttacgatgaa tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc tactttgccc
    26701 gcaggttcgt cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg
    26761 tgggtgttct ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa gcccatatca
    26821 ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcgggaa tttcaataag
    26881 aagaagccga ccttcatttc acctccccga tgcgctcaag cacagactgc accatctgca
    26941 ttcccgtctg acgttcgcga ctgacggctt cgttaaatcg agcgttcagc accggaagct
    27001 ggcctaaggt atccgcaacc atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa
    27061 tcgccatctc tggcagtccc agctgctgca taatcccggc ggatttatgt tcatagttga
    27121 tggcaatcgc cggagtagca aagttcattg agataatggc ggagtgcagg cgcgtaccga
    27181 cggtgagttc acaggctccg agaattttgc ccatttccag atcgttgagt tcatccatca
    27241 ctacgtggta acgggcagga tcgctgatgt gctggcgcag attgagcgcc accatgcggt
    27301 cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatcacc tgatagcctt
    27361 catcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgt tgagtggtgc
    27421 cgagacgttt gtcgaacggt gccagttcgc gcagggtaat tgccactgtt ttctgttgtg
    27481 cggcaacgtc cagccagtgt tgaacggcat agctggcggt gaagtcttcg gtgtggtgat
    27541 cgaccagcca cgcggtatcg acgccatgtt ccacttttgc ggtggtgata tttctgcgtt
    27601 tcatcaaatc aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat
    27661 agttcgccag ttggttaaat tgctcatcct ggaaggggcc gacgctgtga ccaatcataa
    27721 acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga
    27781 gatcgacaaa aaacgatccg ccgacctgaa taatggcgtc gtaacctgac agcaggcgca
    27841 cgaagtcggt gaatccctgg gcgatggcga tattgcgcag cttgccagtg tcggtgacgc
    27901 gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaatg cgcccgacaa
    27961 cgcccgccgc gctattgtgt tgtgtcatct gcaggaacag cggatcgccc attaccgggc
    28021 ggttgagcag ccaggaagaa ctgaccggat agcggctcat cacgtccact tcggtatgtg
    28081 gattgaggat gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc
    28141 cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt tgatgccagg
    28201 tgaggctgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt
    28261 atcaggccta caggtcccga gcacagaacc gtaggacgga taaggcgttt tacgccgcat
    28321 ccggcaaccg ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa gcattttcat
    28381 tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc gtgacagcac
    28441 aatcatcacc acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag
    28501 cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg tcggcaacga
    28561 gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac caagaaccgg
    28621 tttaatcatc acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaaaccgag
    28681 cgtgacgccg attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa
    28741 tgttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga
    28801 accaatcggg ttacccacgg agcgcagcag acccacaata cacagcaatt gcagcaccgg
    28861 aataatgctg ttccactttt cgccgaatac cagcggtacg aagttattcg acaccaccat
    28921 cagccccagc agcgccggaa agttgataat ccccactatc gacagcagct tgtagaagtt
    28981 aacacgcagc ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca acacgcgggt
    29041 gatgattggg ttcagcttca tcggcggtac gacggccacg ttgtacgcca ggttgtatcc
    29101 ccctgccacg cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag
    29161 atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga
    29221 cgccagcgag aagtgcagac cggggcaata aattttgcgt ccaaagtagc caaacagcag
    29281 tgttctcacc gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa
    29341 atgggcgcta actaccgtaa aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc
    29401 gattttgttg aactccagct ccttctgcat caacgcgcgg aactgttgcc cgtgggggat
    29461 caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcagcac
    29521 gtcgccgatg acatcactca acaaaaacac cgccacacat accacaagcc ccagcccgac
    29581 gttcagccag tacagcgtgg tgagttcaag gtgactgatt tcttttcgct gaataatcga
    29641 gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacagt
    29701 cagcaggccg aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc
    29761 gaggccgatg atgatcaccg tggcaatagc cgaccacttc gcgccgctga tggttttttc
    29821 acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa
    29881 cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg aactcgacgc
    29941 gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc
    30001 cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc tggcgatact
    30061 gttcgttatg cgctactgcg tgcggacgtg gaccgacaat cgacatcccc ccggtcagca
    30121 cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc cccactttag
    30181 tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc atcactttca
    30241 tggaacggaa cttccacact ttgatcggct tgccatccat accatagcga gtctggcgga
    30301 aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc aatacagcac agcaccgggg
    30361 agatcagcag cagaataagc gtcgccagca caatgtcttc cgcacgtttt agcaggcggt
    30421 taacccccga aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc atctcttcga
    30481 gacgtgaatg gagaatgttg aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg
    30541 tatccgccag ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga
    30601 tatagacgtt atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg
    30661 cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta aacgcccacc acttcaaacc
    30721 ctaaccacgg ctgattacgg aaactctcca tcagcatttg tccggcggct aaatcccccg
    30781 ctacggcgac catccgcttg ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa
    30841 tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt
    30901 tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta
    30961 aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc
    31021 gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc agcgccacca
    31081 acaggtgcat gtagaggaat gacagtccgc tgacttcgca aaccagccat agcccggcaa
    31141 acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg
    31201 ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag
    31261 tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg
    31321 cttccatcag cggcacatca ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat
    31381 tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat
    31441 ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt
    31501 gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca
    31561 gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca
    31621 tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt
    31681 agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatta
    31741 ccggcgtgcc gcctgcagca gtcaccacat caatagtgtt ccaagagaga cgcggatcat
    31801 ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt
    31861 agtagccctc gataaactgc cctttttcgt caaacagaaa acagcggtca aaatcgccat
    31921 caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac
    31981 attccggcag cagcgggtta ggaataccgt tggggaaatt gccgtccggt gtgttgtgta
    32041 ctttgattaa ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca
    32101 ccggacccgc tgcgccattc ccggagttga tcaccagctt gagcggcgtg agatttttga
    32161 cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc
    32221 gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga cgctggacgt
    32281 cgcgcagtcc ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt
    32341 tgtaatccat cggattatgg ctggcagtaa cttcaatgcc gccatccacg ccgagatgga
    32401 acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg
    32461 catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga cggacatcac
    32521 cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg
    32581 cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg
    32641 ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca
    32701 gttttatttt tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc
    32761 cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc
    32821 cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca
    32881 catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc
    32941 ccgggttttc caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa
    33001 gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt
    33061 ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt
    33121 tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt
    33181 cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca
    33241 catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca
    33301 gatctttcac cccgacggtg gtgaccaggc ctgattcggc atacacatag ctgttttcag
    33361 ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct
    33421 cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag
    33481 catctgccgt aggttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg
    33541 cttcttcatc cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc
    33601 aggcatcgag gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga
    33661 acaggaacat gccgctgttc cagtaatatt cgccgcttgc tacataggcc tgggcggttt
    33721 ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcaccca
    33781 cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc
    33841 caaaggtcac cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga
    33901 acgcgtcttc atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct
    33961 ccgggctatg acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc
    34021 ctgccggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga
    34081 caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca
    34141 ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg
    34201 ggtaaagtac gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg
    34261 gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac
    34321 gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag
    34381 caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac
    34441 cgcaataccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat
    34501 caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc
    34561 cgcgccgcgt ttttgcacca ccagatggca atcgcccatc ttcagcagtg caggtaaagc
    34621 gtcatacgat tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt
    34681 ttccagccgc gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcgcg
    34741 caggagatcg gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga
    34801 gtaaagaatg atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc
    34861 ggcgtctgca acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt
    34921 atccgccgcc acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac
    34981 gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc
    35041 gcctttgcct tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat
    35101 atgcagcacg gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa
    35161 cagcgttggc actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg
    35221 aaagaaactg ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg
    35281 ttttggcacg tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg
    35341 ccaggcggaa tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac
    35401 ccgcacctca tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat
    35461 gccggttaac tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact
    35521 ccggcacgct tctcagcgag aaaataggct cggctgttag cgtgaacatc gttactggcg
    35581 agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc
    35641 tcttcttccg ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg
    35701 ccggagaagt tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc
    35761 ggcagacgca gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg
    35821 tctttctgca cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt
    35881 ttgccaagca gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt
    35941 gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc
    36001 ggtcttgatt ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt
    36061 cgtgatacca gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat
    36121 ccggtttgct ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt
    36181 gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg
    36241 gttgggtatt ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg
    36301 ccatatcatc gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt
    36361 ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg
    36421 agttactcgg gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt
    36481 aatcgcgtcc atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa
    36541 tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg
    36601 gctgttttgc cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt
    36661 cgttctgatg cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat
    36721 ccgccggata ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct
    36781 ggtcaatgcg ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt
    36841 catcgcgggt acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg
    36901 cagaaccgac catcccacaa tggccagcga taaaaatgcg ttgtttactc atgcttatga
    36961 ctccagcgcg atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc
    37021 ttcgaggtca ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca
    37081 gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg
    37141 gaagtaacgc ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg
    37201 cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt
    37261 gatgcccagc tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt
    37321 cgcgataacg aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta
    37381 gtctttggcg tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc
    37441 cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc
    37501 gcggcgcggg gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc
    37561 gcggtagtta acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg
    37621 gtagaacggc gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt
    37681 ggaagcctga tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag
    37741 gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc
    37801 aacgtggctc attgcgccta ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg
    37861 cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt
    37921 gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt
    37981 aataccatgc acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg
    38041 tccggttaca ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa
    38101 tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat
    38161 ttgccggaag cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg
    38221 tgacgcctgg ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag
    38281 gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac
    38341 ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga
    38401 cgtcatcgcc aacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta
    38461 ctgacggacg aataactacg ttttttccta tttttgctcc gaataaacgt aataaaaaag
    38521 cccgccagcg atacaatact tgtggcgacc aggcaaatat tgttgcctgt actgcccacc
    38581 ataattgcac tttaatagcg ttgccgcccc ggaacccttt cggcaccgag aaaccgctta
    38641 aatcttgcat tggttttcct tatattcaga ctttgttata taaggcttta gtcttacctg
    38701 tcgtacgttg gcgtaaatgc caggataatt ctgcccagaa gccaggtaca tgtaatatct
    38761 gccgttggac ttttttggcg tcagcac
//
LOCUS       DAJTNP010000028        38688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTNP010000028 DAJTNP010000000
VERSION     DAJTNP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..155
                     /locus_tag="OO160_001603"
     CDS             <1..155
                     /locus_tag="OO160_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5523772.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO160_001604"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO160_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5523773.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO160_001605"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO160_001605"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5523774.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO160_001606"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO160_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO160_001607"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO160_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5523775.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO160_001608"
     CDS             complement(5626..7134)
                     /locus_tag="OO160_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5523776.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO160_001609"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO160_001609"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5523777.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO160_001610"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO160_001610"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5523778.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO160_001611"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO160_001611"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5523779.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO160_001612"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO160_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5523780.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO160_001613"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO160_001613"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5523781.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO160_001614"
     CDS             complement(14283..15602)
                     /locus_tag="OO160_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5523782.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO160_001615"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO160_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5523783.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO160_001616"
     CDS             16699..17034
                     /locus_tag="OO160_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5523784.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO160_001617"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO160_001617"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5523785.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO160_001618"
     CDS             complement(17790..19172)
                     /locus_tag="OO160_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5523786.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO160_001619"
     CDS             complement(19358..20302)
                     /locus_tag="OO160_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5523787.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO160_001620"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO160_001620"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5523788.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO160_001621"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO160_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5523789.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO160_001622"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO160_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5523790.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO160_001623"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO160_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5523791.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO160_001624"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO160_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5523792.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO160_001625"
     CDS             complement(25947..26768)
                     /locus_tag="OO160_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5523793.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO160_001626"
     CDS             complement(26868..26951)
                     /locus_tag="OO160_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523794.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO160_001627"
     CDS             complement(27044..27379)
                     /locus_tag="OO160_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5523795.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO160_001628"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO160_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30065
                     /locus_tag="OO160_001629"
     CDS             29136..30065
                     /locus_tag="OO160_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5523796.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAKEPFVFFDPHVGTGLY
                     DDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAE
                     EDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30200..31420
                     /gene="mlc"
                     /locus_tag="OO160_001630"
                     /gene_synonym="dgsA"
     CDS             30200..31420
                     /gene="mlc"
                     /locus_tag="OO160_001630"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5523797.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31545..32240
                     /gene="bioD"
                     /locus_tag="OO160_001631"
     CDS             31545..32240
                     /gene="bioD"
                     /locus_tag="OO160_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5523798.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO160_001632"
     CDS             complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO160_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5523799.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO160_001633"
     CDS             complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO160_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5523800.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34301..35155)
                     /locus_tag="OO160_001634"
     CDS             complement(34301..35155)
                     /locus_tag="OO160_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5523801.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO160_001635"
     CDS             complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO160_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5523802.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35785..37823)
                     /locus_tag="OO160_001636"
                     /pseudo
     CDS             complement(35785..37823)
                     /locus_tag="OO160_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37884..>38688)
                     /locus_tag="OO160_001637"
     CDS             complement(37884..>38688)
                     /locus_tag="OO160_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR5523803.1"
                     /translation="FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWL
                     QHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIE
                     IYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHST
                     YGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSA
                     MGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9832 a         9374 c         9918 g         9564 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaagcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagagt acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgtgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt
    29761 ttgatccgca cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc
    29821 atttgacgcc cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt
    29881 ccgccggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa
    29941 tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga
    30001 aacatcatga acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca
    30061 ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta
    30121 aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg
    30181 aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa
    30241 gcagaccaac gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat
    30301 cgatctttcc cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat
    30361 gctcgaagca cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc
    30421 ggcggtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg
    30481 cggggagatt ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca
    30541 ggaactggcg ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca
    30601 gttttttatc cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc
    30661 gggaattatt gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa
    30721 agagatgccg ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca
    30781 tgatatcagc gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga
    30841 tgtgattcag gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct
    30901 gctacacgca ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg
    30961 taaacgctgt tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat
    31021 tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc
    31081 gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat
    31141 cattatcggg gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa
    31201 cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt
    31261 catctcagac agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga
    31321 gagtactcag ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat
    31381 gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc
    31441 aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt
    31501 attttcctag tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct
    31561 ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag
    31621 cgttagcctc ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag
    31681 agacacccga agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg
    31741 aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc
    31801 acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag
    31861 tcgatcatgt cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc
    31921 cactctctga atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg
    31981 aaggttgcat taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc
    32041 tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg
    32101 atgtgctggg taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg
    32161 ccgaacagcg tgaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg
    32221 tagatagagt cacggtgtaa cgtctgcgaa attaccgacg caattacgca ggcaatcaat
    32281 aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc
    32341 ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta
    32401 atttcttcgc catcagggaa ccataatccc aggctacgac catacaacat gccaatggca
    32461 agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc
    32521 agcacggcac acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg
    32581 gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa
    32641 agcagaccca cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc
    32701 acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc
    32761 agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt
    32821 acgttgtaga gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg
    32881 gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct
    32941 ataaataaac tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca
    33001 caggcgatcc ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg
    33061 gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact
    33121 accagcagcg aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt
    33181 tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag
    33241 cccatcagca acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag
    33301 gaggaaaggt ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac
    33361 tccagtagca gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg
    33421 actgttgcga taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga
    33481 tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac
    33541 cgcaaaagca gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc
    33601 gaatcgcgcg taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt
    33661 taaccgcgac aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt
    33721 cacccagtgc acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg
    33781 ttgaccacgg aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct
    33841 ccgccaacca cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct
    33901 tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc
    33961 caaacagcac agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc
    34021 acgggccgac aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa
    34081 aagccgtcac cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt
    34141 cactggtgag taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca
    34201 gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc
    34261 ctaacatcct gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc
    34321 ctactgtcat gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga
    34381 ggattagccc cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa
    34441 gccagcatcc aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat
    34501 ctggtaccag tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt
    34561 gcagcacaat cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg
    34621 tggtattaaa tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca
    34681 acaataccgt caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt
    34741 cgatttgata cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa
    34801 gtaaccagag tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata
    34861 gtccgccaac ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc
    34921 cgattcgatt cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa
    34981 taaacccgat gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt
    35041 ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc
    35101 attgacccaa tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac
    35161 acttcctccg gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta
    35221 ggtttgataa cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc
    35281 agagtgccgt gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg
    35341 caggattcga cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac
    35401 ttcgtcatgt gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg
    35461 cagtagcggc agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta
    35521 tgcattgcgc cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag
    35581 atggagagat aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg
    35641 ccgccagcat attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct
    35701 ttgcacgcca gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca
    35761 tattgtgtgg tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga
    35821 cgggtttccc tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc
    35881 gtgatcgacc cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt
    35941 aacgccaggc agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt
    36001 aaatacccgc acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat
    36061 ccacacctct tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt
    36121 acgcgctttg gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt
    36181 gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag
    36241 ataaggtctt cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc
    36301 gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc
    36361 acctcatggg tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac
    36421 cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg
    36481 gtcattttcg taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta
    36541 acaggattct cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca
    36601 cctgagttgc cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg
    36661 cgggacgttt gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca
    36721 ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg
    36781 atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag
    36841 gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca
    36901 caatatttgt cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc
    36961 gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg
    37021 gcagctgtgt cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg
    37081 gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt
    37141 ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca
    37201 ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg
    37261 agaaaacccc cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg
    37321 ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc
    37381 cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta
    37441 ccttcaccgc gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc
    37501 cgtcggcgga tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg
    37561 tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc
    37621 agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga
    37681 gcctctgccg cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg
    37741 agactgccaa gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc
    37801 gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg
    37861 ccagaattta tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc
    37921 gggtttccct tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca
    37981 tggtcgattt tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta
    38041 actccaggga gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta
    38101 aacacccgca ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc
    38161 cacacctcct gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta
    38221 cgggatttgt aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc
    38281 cagccttcaa aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt
    38341 tcccaggtac gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc
    38401 gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca
    38461 ttgggatctt tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc
    38521 ggatctttgg caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct
    38581 tctgtgaact tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag
    38641 taaatcggtt tacgttcgaa tttttcgctg gtgactggct ggaggaaa
//
LOCUS       DAJTNP010000029        37591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTNP010000029 DAJTNP010000000
VERSION     DAJTNP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..139)
                     /locus_tag="OO160_001638"
     CDS             complement(<1..139)
                     /locus_tag="OO160_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5523804.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LK"
     gene            complement(223..1107)
                     /gene="yhdJ"
                     /locus_tag="OO160_001639"
     CDS             complement(223..1107)
                     /gene="yhdJ"
                     /locus_tag="OO160_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5523805.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1193..1489)
                     /gene="fis"
                     /locus_tag="OO160_001640"
     CDS             complement(1193..1489)
                     /gene="fis"
                     /locus_tag="OO160_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5523806.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1515..2480)
                     /gene="dusB"
                     /locus_tag="OO160_001641"
     CDS             complement(1515..2480)
                     /gene="dusB"
                     /locus_tag="OO160_001641"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5523807.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2809..3690)
                     /gene="prmA"
                     /locus_tag="OO160_001642"
     CDS             complement(2809..3690)
                     /gene="prmA"
                     /locus_tag="OO160_001642"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5523808.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3702..5153)
                     /gene="panF"
                     /locus_tag="OO160_001643"
     CDS             complement(3702..5153)
                     /gene="panF"
                     /locus_tag="OO160_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5523809.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5143..5385)
                     /locus_tag="OO160_001644"
     CDS             complement(5143..5385)
                     /locus_tag="OO160_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5523810.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5494..6843)
                     /gene="accC"
                     /locus_tag="OO160_001645"
     CDS             complement(5494..6843)
                     /gene="accC"
                     /locus_tag="OO160_001645"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5523811.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6854..7324)
                     /gene="accB"
                     /locus_tag="OO160_001646"
                     /gene_synonym="fabE"
     CDS             complement(6854..7324)
                     /gene="accB"
                     /locus_tag="OO160_001646"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5523812.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8302..9276)
                     /locus_tag="OO160_001647"
     CDS             complement(8302..9276)
                     /locus_tag="OO160_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5523813.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9428..11368
                     /gene="csrD"
                     /locus_tag="OO160_001648"
     CDS             9428..11368
                     /gene="csrD"
                     /locus_tag="OO160_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5523814.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11673..12716
                     /gene="mreB"
                     /locus_tag="OO160_001649"
     CDS             11673..12716
                     /gene="mreB"
                     /locus_tag="OO160_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5523815.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12782..13885
                     /gene="mreC"
                     /locus_tag="OO160_001650"
     CDS             12782..13885
                     /gene="mreC"
                     /locus_tag="OO160_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5523816.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13885..14373
                     /gene="mreD"
                     /locus_tag="OO160_001651"
     CDS             13885..14373
                     /gene="mreD"
                     /locus_tag="OO160_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5523817.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14382..14975
                     /gene="yhdE"
                     /locus_tag="OO160_001652"
     CDS             14382..14975
                     /gene="yhdE"
                     /locus_tag="OO160_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5523818.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14965..16434
                     /gene="rng"
                     /locus_tag="OO160_001653"
     CDS             14965..16434
                     /gene="rng"
                     /locus_tag="OO160_001653"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5523819.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16502..20302
                     /gene="yhdP"
                     /locus_tag="OO160_001654"
     CDS             16502..20302
                     /gene="yhdP"
                     /locus_tag="OO160_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5523820.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20458..21903
                     /gene="tldD"
                     /locus_tag="OO160_001655"
     CDS             20458..21903
                     /gene="tldD"
                     /locus_tag="OO160_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5523821.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22031..22960)
                     /gene="aaeR"
                     /locus_tag="OO160_001656"
     CDS             complement(22031..22960)
                     /gene="aaeR"
                     /locus_tag="OO160_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5523822.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23143..23346
                     /locus_tag="OO160_001657"
     CDS             23143..23346
                     /locus_tag="OO160_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5523823.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23354..24286
                     /gene="aaeA"
                     /locus_tag="OO160_001658"
     CDS             23354..24286
                     /gene="aaeA"
                     /locus_tag="OO160_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5523824.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24292..26259
                     /gene="aaeB"
                     /locus_tag="OO160_001659"
     CDS             24292..26259
                     /gene="aaeB"
                     /locus_tag="OO160_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5523825.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26351..26623
                     /locus_tag="OO160_001660"
     CDS             26351..26623
                     /locus_tag="OO160_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5523826.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26679..26942)
                     /gene="yhcN"
                     /locus_tag="OO160_001661"
     CDS             complement(26679..26942)
                     /gene="yhcN"
                     /locus_tag="OO160_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5523827.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27307..27777)
                     /gene="argR"
                     /locus_tag="OO160_001662"
     CDS             complement(27307..27777)
                     /gene="argR"
                     /locus_tag="OO160_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5523828.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28212..29150
                     /gene="mdh"
                     /locus_tag="OO160_001663"
     CDS             28212..29150
                     /gene="mdh"
                     /locus_tag="OO160_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5523829.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29212..30279)
                     /gene="degS"
                     /locus_tag="OO160_001664"
     CDS             complement(29212..30279)
                     /gene="degS"
                     /locus_tag="OO160_001664"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5523830.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30369..31716)
                     /gene="degQ"
                     /locus_tag="OO160_001665"
                     /pseudo
     CDS             complement(30369..31716)
                     /gene="degQ"
                     /locus_tag="OO160_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31870..32268)
                     /locus_tag="OO160_001666"
     CDS             complement(31870..32268)
                     /locus_tag="OO160_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5523831.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32462..33589
                     /gene="zapE"
                     /locus_tag="OO160_001667"
     CDS             32462..33589
                     /gene="zapE"
                     /locus_tag="OO160_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5523832.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33808..34236
                     /gene="rplM"
                     /locus_tag="OO160_001668"
     CDS             33808..34236
                     /gene="rplM"
                     /locus_tag="OO160_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5523833.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34252..34644
                     /gene="rpsI"
                     /locus_tag="OO160_001669"
     CDS             34252..34644
                     /gene="rpsI"
                     /locus_tag="OO160_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5523834.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35039..35677
                     /gene="sspA"
                     /locus_tag="OO160_001670"
     CDS             35039..35677
                     /gene="sspA"
                     /locus_tag="OO160_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5523835.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35683..36180
                     /gene="sspB"
                     /locus_tag="OO160_001671"
     CDS             35683..36180
                     /gene="sspB"
                     /locus_tag="OO160_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5523836.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36223..37590)
                     /gene="dcuC"
                     /locus_tag="OO160_001672"
     CDS             complement(36223..37590)
                     /gene="dcuC"
                     /locus_tag="OO160_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5523837.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9104 a         9405 c         9896 g         9186 t
ORIGIN      
        1 gttttagtgg attaactgag ttcatattac ggcatttgtc tgtttctgtt gtcagcagtt
       61 caatccactg catcagtaaa gtatcaaaca gaaaaacgaa gacagcgaaa acgacgagcc
      121 accaatactt gcgaatcatt acctgagcct gtgcatgttt aaccaagtct ggcaggaaat
      181 atacacgaaa tgaataatct gaaaactagg cttacacttc ctttactttg caatgagatc
      241 ggggtcaact tcgcttaacc ggcttcgttt tgacaggttg ccggtctttc tttttttcac
      301 tttcgccagt tcttccgcgg agtaatgcga cgcgacatcc agccgtcgaa gccccatttt
      361 gatgtactcg ctgttgatct caataccaat gaattttcgt ccgctggcga tggctacggc
      421 accggtagta aagctaccgg caaacgggtc gagaacgata tcgcctgggt tggaagaggc
      481 gagaataatg cgtttcagta aggctttcgg tttttgcgtc gggtggttct catattcatc
      541 catcaaataa cgcacgcgcg gaaaatccca gacgttaccc ggtacttttt gatgattgta
      601 tggctgtgga ggatttttgc gataatcgat caacgcgcgc tgcgatccgg ttttggcttc
      661 taccagaata gcatcaccgt tgaatgtgta gttctttgcg tctttcacca tcatcaggat
      721 gggttcgtac atggagccgt agtgtttttt cgcctgtact ccagaactgt catatgacca
      781 gacgatgcga cttttgatgg taaaaagctt gcggcactgg agatcgataa agggcatgtt
      841 ttccgtactg ttcataatgt acatgctgcc ctgctttttc agaacgcggt ggcactctgc
      901 aatcacttca aacagccagt cgataaacag atcttctttc caggcttcga tcagaccatc
      961 aaaattttta ccgatgttat acggtgggtc ggcaaagagc agatcgacgc tttcagtagg
     1021 tagcttttta agttcggcaa aggcatcacc gtgaataatg gtcttagctt cattaccaaa
     1081 ccgggtcggt tcacatcctg ttctcatggt cactcccttt gtgacaccta taaaaaaggc
     1141 gcttccccat gccgagtagc gcctttttaa tcaagcattt agctaacctg aattagttca
     1201 tgccgtattt tttcaatttt ttacgcagcg taccacggtt gatgcccatc atcagcgcag
     1261 cacgggtctg gttaccacgg gtgtattgca tcaccatgtc caacaggggc tgttctactt
     1321 cagccagtac cagctcatag aggtcattca catcctgacc attcagttga gcaaaatagt
     1381 tcttcagtgc ctgtttaacc gagtcacgca ggggtttttg ggttacctga tcctgagagt
     1441 taacggtaga aacggtcagt acgtcagaat ttacgcgttg ttcgaacata gttctgtcag
     1501 ctctttattt ctgtttacgc aaaattttcg aagtatgcct ccaacgcctc cagctgttcg
     1561 ctggcatcct caatggcgtt gaatgtgcgc cgaaactggt catttggagc gtgttcctgg
     1621 agataccagg aaacgtgttt acgtgcaatt cggtaccctt ttgccggacc ataaaagtca
     1681 tgcagttccc gaacgtgcgc gcaaagcaag cgcttaacct ctgccaaagg cagtgggggc
     1741 agcaactccc cagtgtccag ataatgctgg atttcccgaa agatccaggg tcttccctga
     1801 gctgcgcggc ctatcatcag ggcatccgcc cctgtatagt cgagcacagc tctggcttta
     1861 agcgggtcag taatgtcgcc attcgcgata accggaatgg aaactttctg cttaactgcc
     1921 cgaatactgt cgtactcagc ttctccattg aacaaacagg cgcgtgtacg gccatgaatg
     1981 gtcagagcct gaatgccaca gtcttcagcc agttgggcaa tctcttcgca gttacggtgt
     2041 tccggtgccc agccggtgcg aatcttcagg gtaacaggaa cgtccactgc attgacgacc
     2101 tcggtaagga tcgatttaac gacatccggg tactgcaaga gggctgaacc tgcgagcttg
     2161 cgattcactt ttttagccgg gcaacccata ttgatatcaa taatctgggc accgctttcc
     2221 acgttaatac gtgctgcatc tgccatttct ttcggagcgc taccagcaat ttgcacggtg
     2281 cgaataccgg gttcatcaat gtgcaccatc cgtaaacgag atttgtcgct ttcccaaacc
     2341 tgtgggttag aagacatcat ctcggatact gtcaatccgg ctcccatctc gtagcacaac
     2401 gtccgaaaag gtctgtctgt aatgccagcc atgggcgctg cgatcaggcg atttctgagc
     2461 tgatattgtc cgatgcgcat gagttaagaa atgaccatac tgtgactgca aggcggcgta
     2521 tattacgcat tttttgcacg agatgaaagg ccaaactttg accaatcctc tgagatggat
     2581 caaagaattg catttaaaat gagcgtggtg cgataattac tcataaaaat catcatatta
     2641 gaaaatagtg actaaaattt acactcaaag aaatttgagt aagttctcaa tttttcttta
     2701 tgaatgaaaa ttttggcacg caaatttgcg taaataatcg gcaaatttac gtgccttttg
     2761 tgagcttgct cgcacttcgc cccgcgtcac cctacggcga tgcgaaggtc aattcttacg
     2821 accggtaata cggcaccact cttctttttc cacgaccggg tccagtgcga agctatcggc
     2881 ataagcttca caaacgctct ctgcctggct tgccagaata ccggaaaggc ccagcaaacc
     2941 gcctgaaact ggcaggacgc tgattaacgg tgccagttca cgtaatgggc ctgcaaggat
     3001 gttagcgacc accacgtcgg ctttcatttc ttctggctga tctttcggta agtagagttc
     3061 cagacggtca gaaacgccat tacgttcggc gttatcgcgg ctggcctgaa tcgcctgcgg
     3121 atcgatatca ataccaatgg cttttgctgc acccagtttc agcgccgcga tcgccagaat
     3181 gccggaacca cagccaaagt cgatgactgt tttaccggtt aaatcgaggc tgtcgagcca
     3241 ttgcaggcac agagaggtgg ttggatgggt acccgtacca aacgccagcc ctggatctaa
     3301 catcacgttg acggcgtttt cgtccggcac atcacgccag ctagggcaga tccacagtcg
     3361 ttcaccaaag cgcatcgggt ggaaattatc catccattcg cgctcccagt ctttatcttc
     3421 tagttgttcg attttatgcg cgaagcctgc gccgagcagc ggatggtttt ccagaatcgc
     3481 caccacgtcg ttcatatcgg tttcagcgtc gaacagacca atcacatcgg tgtcgcccca
     3541 caggcgcgtt tcgcccggca gcggctcaaa tactggcgta tcgtgggtat cctgaaaagt
     3601 gatagaaacg gcacccgctt ccatcagcgc atcgctaaga tcttccgcgt tcgcgccggt
     3661 ggtgttcagt ttcagttgga tccaaggcat ggcaaaactc tttatttatc agtagtcaaa
     3721 acggtagctt gcgggacgga tgtaccgaaa cggtttccga ccaggaaagc cagcaaactt
     3781 agtagtaacg agggcacgat agggtggaag cccaggtact gaatattcag cgtcgcgagt
     3841 acggcataca gcacgccgcc aacgatcatc gcacttagcg cgcctttggc gttggcgcgt
     3901 tcccagtaaa gacccagcac cagcggccac aggaaaacgg cttccagccc accgaaggcc
     3961 agcaaattca gccagatgat catttctggc ggcttccagg cggcaagcag cagcaacgcg
     4021 ccgagaacta acgtaattac cgccgacatc cgcttcagac gcgtctcgtt ttgcatttga
     4081 tccggacgga tattcagata gagatcttta atgatcgtag cggaactttg cagcagttgg
     4141 gcgttaattg tcgacatgat cgcagccata ggtgcagcca gaaagatccc ggcagcaaaa
     4201 ggtggcagca cttttaccat taacgtcggg atcaccagat ctggtacggt gagatcgggg
     4261 atcactgcac gacctaacgc tccggccagg tgcataccga acatcaggat tgcgactata
     4321 atcgtaccga tgatgatccc ccggtgtact gctttgctgt ctttataaga gatacagcgc
     4381 accgcagtat gcggcaggcc aatcacgcca aagcacacca gtacccagaa cgacgtcata
     4441 aaggcaggcg acagaatatc gtcagcgcct tgtggcgtaa ccagttgcgg atcgatggtt
     4501 tgcaaggtct gtactgcgtt gcttaagccg ccagcggcat gtaccacgcc aataagcagc
     4561 acaacggtgc caatcagcat cacaagccct tgcatggtgt cgttcagcac gctggcgcga
     4621 aagccaccaa aggcggtata taacgcaatg ctgataccaa aaatcagcag cccggtttca
     4681 taaggaatac ccgccgcggt ttccagcagg cgcgcaccgc cgataaactg cacggtcatc
     4741 gcaccaacga acgcaaccag caaactcaaa ctcgccagcc acaccagaag acgactctgg
     4801 tagcgggcaa acagcatatc gttcagcgtc actgcattgt agcggcgcgc aagaatcgca
     4861 aacttcttgc cgagaatacc gagtgaaagc cagacagcag gaagctgaat catcgccagc
     4921 aatacccagc ccagcccgta tttataagca gctcctggcc cgccgataaa cgaactggca
     4981 ctgatatagg tcgcggtgag agtcatcgcc agcacaatac cgcccataga gcggctgccg
     5041 aggaaatact cattaaggaa ggtgccggtg ctccgtttac gcatcgcata aaccgagata
     5101 ccgaacacca ccaccagata ggcgaccagc ggtagaatta cttcaagctg catcgtcatc
     5161 ctccagtggg atatcgcgat agataaattt caccatcgcc cagcacagtc caataaacag
     5221 tagcggcgtc aggatgcagg ccatctcaaa ccagcgcgga aagccggtaa aaccgggggc
     5281 aacgccaggt aagtaagcgt ctactaacca aactgccaga tacaaaaggg tcagccccag
     5341 cgcccagcgg gcctctttat gggcctgaac aaaacgagtg tccatttttt gtccctgatg
     5401 ggtgaagaaa gcgaggattg taccttatgg gggttgtcga tccccagtaa taaaaaaggc
     5461 cggaaaatcc ggccttttga cgcttttgca gtcttatttt tcctgaagac cgagtttttt
     5521 ctccagatag tggatgttag tgccaccatg ctggaagttc tcgtcattca tgatgcggat
     5581 ctgcagatca acgttggttt tgataccgtc gatgatcagc tcctgcagcg cattcttcat
     5641 gcgggcaatc gccacgtcac ggttttcacc gtagcaaatc agcttaccga tcattgagtc
     5701 atagtacggc ggtacggtgt agcccgcgta gatatgagac tcccaacgta cgccaaaacc
     5761 gccaggtgcg tggaaacggg tgattttgcc tgggcttggc aggaaggtgt tcggatcttc
     5821 ggcgttgata cgacattcca ccgcatggcc gcgaacgtga acttcttctt gcttgatcga
     5881 cagcggttga ccggcagcga tacgcagctg ttctttgatc aggtcaacgc cggtgatcat
     5941 ttctgtaacc gggtgttcta cctgaatacg ggtgttcatt tcgatgaaat agaactcgcc
     6001 gttttcgaac aggaactcga aagtacctgc accgcgatag ccgatatcaa cacacgcttt
     6061 agcgcaacgt tcgccgatgt agcgacgcag ttccggggta atgcccggtg ctggtgcttc
     6121 ttcgaccact ttctggtggc ggcgctgcat ggagcagtca cgttccgcca gatagatagc
     6181 gttgccctga ccgtcagcca gtacctgaat ctcgacgtgg cgaggattct ccaggtattt
     6241 ctccatgtaa accatatcgt tgctgaaagc agctttcgct tccgcacggg tcatggagat
     6301 ggattgtgcc agttcagcgt cgccgcgcac tacgcgcata ccgcgaccgc cgccgccgcc
     6361 ggaggctttg ataatcaccg gataaccaat gcgtttagca atggcgcggt ttttatccat
     6421 atcgtcgccc agcgggccgt cagaaccagg tacgcaagga acgcccgctt ttttcatcgc
     6481 cgcgattgcg gatactttgt cgcccatcag gcgaatggtt tctgctttcg ggccgatgaa
     6541 gataaagccg gagcgttcaa cctgctcggc aaagttggcg ttctcggaga ggaagccgta
     6601 acccggatgg attgctactg cgccggtgat ttcagcggcg ctgatgattg ccgggatgtt
     6661 cagataactt tttactgacg gagcagggcc aatacagacc gtttcatctg ccagtaatac
     6721 gtgttttaga tcgcgatccg tgctggagtg cacagcgaca gtcttgatgc ccagttcttt
     6781 acaggcacga agaatacgca atgcaatctc gccgcggttg gcaataacaa ttttatccag
     6841 catgttcgcc tcgttactcg atgacgacca gcggctcgtc aaattctacc ggttgtccac
     6901 tttcgaccag aattgctttc acggtaccgg atttgtccgc ttcgatctgg ttcatcattt
     6961 tcatggcttc aacgatgcac agggtatcgc ccacgttgac tttctgaccc acttcgatga
     7021 acgcttttgc gtccgggctt ggggtgcggt agaaagtacc aaccatcggg gaacgtacga
     7081 tgtgaccact gatttccgct gctgctggcg cttccatgga aggaacggtc gccggagcgg
     7141 ctgcgttaga ttgagctggc tgctgcatca ttggtgcagc gtaagcttgt tgcatcacag
     7201 ggaaacttgc ggcaggagct gcacggctaa tgcgtactga ctcttcgcct tcagaaattt
     7261 ccagttcgga gatgcctgat tcttcaacca gctcgatcag ttttttaatc ttacgaatat
     7321 ccatgagtgg gttccgtact ctttgtttag tgtgattgtg acaggcgttt caccgccgtc
     7381 tgtaaagcgt atgccccgac agctgtatgc atagcgataa attccagcag gccgggacgc
     7441 cggtctattt tgccccagag tgtcaatgtt agacttgacg gacattgtgc agcgatctgc
     7501 tgctatcctc cggcaaaaaa caaaatatac cttcgttgcg ccaatgtcac cttttccgca
     7561 gcgcaaaaac tgcgtcaggg aggacgaggt cgcacattat aacgatttcg tagcaattgg
     7621 cagctaaata ctggtcttat cagggaatat aatcaacagc taacatgtaa ataaccttca
     7681 acaccgtgta atttgcaaca agccgcacaa ttcacgaaat tagcgccatc atcgacggaa
     7741 cttcttataa cgtaaggtga aaagctgaaa agccagccct gcgaagatga ggggctgcaa
     7801 tgagataatc ttcacagacc acaaataatg tagggatgcc aggatcgcga caagatagac
     7861 gacattacgc agcggttgct ggggattacc caattttcac tgcatcgtct ggatagatgt
     7921 gaatgctaaa gcacgcaaaa tcaaccaact gacaattctc agcattaaat aggttcgagt
     7981 ggttaatttg tagtcaaata aaatcagatt attcacccca tggtaccaga aatcagtaac
     8041 aggttaagcg tagtgtcgcc caggcaaagc accataaacc taacaggcga cggaggcgta
     8101 tcaataatgg ctgtttagag gagtatcaat gcgcatcaat ttgtggtcag tcgaaatcat
     8161 gactttccag tttggatgat gttgtacaac ttcctgacca aggtgttcga cgaaagctgt
     8221 ccggctaacc gcttttcccg ttaaccgatt gaataatgat gtcaaaatga gttccctctc
     8281 ttattattcc ttgctaaatg gttagttaac cttcaccagc gtgcgaccct ggatctggtt
     8341 attaatgatg gcctcggcaa agttcggtgc ctctgacaga gatatctctt ttgccgcctg
     8401 ggtatagaat gattccggta aatcggcgac cagtcgctgc caggcttgtg cgcggcgttc
     8461 tggtggcgtc attactgaat ccaccccttg caaacggaca ttacgcagaa taaatggcat
     8521 gaccgtggtt ggcagagtaa aaccacccgc cagaccacag gccgccacgc agccgccgta
     8581 attcatttgc gccagcactt ttgccagcac tttgtcgcca acagtgtcaa ttgccccagc
     8641 ccagacttgt ttttccagag gacgggattc ggcaaactca tcacgaggga gaataccgct
     8701 ggcacctaaa cttttcagat attcatgggt actttcgcga ccggaaacgg caacgacctg
     8761 ataacccaac ttatgcagca gcgccacggc ggtactgccg acgccaccac tggcacccgt
     8821 cacgacaatc tcaccgtcct gcgggcgaac accggcatct tccagcgcca tcacacacag
     8881 catggcggta aaaccggcag taccgataat cattgcttta cgcgcgtcca gcccttgcgg
     8941 catggcaacc agccagtcac ctttcactcg cgcctgctcc gccagcccac cccagtggtt
     9001 ttcaccaacg ccccagccag tgagtaacac ctcctgaccg gcatgaaaac gcggatcttc
     9061 gctggtgcgt acagttccgg caaaatcgat cccaggaatc atcggaaaat tacggatgat
     9121 ttttccctta ccggtaatcg ccagcgcatc tttatagttc aggctcgacc agtgaacatc
     9181 gaccgtgaca tcgccctccg gcaggcgacg ttcgtccaga gtctgtactg atgcgagagt
     9241 tttgccgtcc tgctgttcta aaagtaacgc ctgcataagt ggtcctcatg tgcatgatgg
     9301 attggaaaat aatttctgaa gactatactc gctaatggaa aagcaaagcc gatgaagcgc
     9361 aataaattgc gagataaatc tgatttgcta gtatgcccgc ttcctcacta tcggagttaa
     9421 cacaaggatg agattaacga cgaaattttc ggcctttgtt acgctgctca ccgggttaac
     9481 aatttttgtg actttgctgg gctgttcgct aagtttctac aacgccattc agtataagtt
     9541 tagtcatcgc gttcaggcgg tggcgacggc gattgatacc caccttgtgt cgaatgactt
     9601 cagcgcatta aggccacaaa ttaccgaatt aatggtgtca gcagatatcg ttcgtgtaga
     9661 cctgctgcat ggtgataaac aggtttatac cctggccaga aatggtagtt atcgtccggt
     9721 tggcaccagc gatctgtttc gtgaactgag cgttccgttg ataaagcatc cggggatgtc
     9781 gttgcgtctg gtttatcagg atccgatggg caactatttc cattcgttga tgaccaccgc
     9841 gccgctcacg ggggcgattg gctttatcat tcttatgctc tttctggcgg tacgctggtt
     9901 acaacggcaa cttgccgggc aagaattgct ggaaacccgg gctactcgta tcttaaacgg
     9961 tgagcgtggc tctaatgtgt tgggaaccat ctatgaatgg ccgcccagaa ccagcagtgc
    10021 gctggatacg ctgcttcgtg aaattcagaa cgcacgcgaa caacacagcc gtcttgatac
    10081 gctgatccgc tcttatgccg cacaggacgt gaaaaccggc ctcaataacc gactcttttt
    10141 cgataatcag ttagcaacgt tactggaaga tcaggagaaa gtaggtaccc acgggatcgt
    10201 gatgatgatt cgtctgccgg atttcaatat gttgagcgat acctgggggc acagccaggt
    10261 tgaagaacag ttcttcactc tgacgaatct gctgtcgaca tttatgatgc gctaccctgg
    10321 cgcactgctg gcgcgttacc accgcagtga ttttgctgcg ctgttaccgc accggacgtt
    10381 aaaagaggca gagagcatag ccagtcagtt aatcaaagcc gtcgatacct tgccgaacaa
    10441 taaaatgctc gatcgcgacg atatgatcca cattggtatc tgtgcctggc gtagtggtca
    10501 ggataccgag caggtaatgg aacatgcaga gtctgccacg cgtaatgcgg gattgcaggg
    10561 tggcaatagc tgggctattt atgatgactc gttgcctgaa aaaggacgcg gtaatgttcg
    10621 ctggcgtacg cttatcgagc aaatgctcag tcgcggcggc ccgcgccttt atcaaaaacc
    10681 ggcggttact cgcgaaggtc aggttcatca tcgcgaactc atgtgccgca tcttcgatgg
    10741 taatgaagag gttagctcgg cggagtatat gccgatggtc ttgcagtttg gcttatcgga
    10801 agagtatgac cgtctgcaaa tcagccgtct tattccacta ttgcgttact ggccagagga
    10861 aaatctggcg attcaggtta ccgttgagtc gctgattcgt ccgcgttttc agcgttggct
    10921 gcgcgatacg ttaatgcaat gtgaaaaatc acaacgaaaa cgcataatta ttgaacttgc
    10981 agaggccgat gtaggtcaac atatcagtcg tttacaacct gttattcgtt tagtgaatgc
    11041 tttaggggta cgggtagccg tcaaccaggc tggtttgacg ctggtaagca ccagttggat
    11101 caaagaactt aatgttgagt tactcaagct ccatccgggg ctggtcagga acattgagaa
    11161 gcgaacggag aaccagctgc tggttcaaag cctggtggaa gcctgctccg ggaccagcac
    11221 ccacgtttac gccaccggcg tgcgttcgcg aagcgagtgg cagaccctga ttcagcgcgg
    11281 tgttacaggc gggcaagggg attttttcgc gtcctcacag ccacttgata ctaacgtgaa
    11341 aaaatattca caaagatact cggtttaacc tgccgtttaa tccgttttca cgtagaataa
    11401 cgcgcgctgc gtctcatggg agtgtgcttg tctgctcgcc agattgttgc agcacatatg
    11461 cagatgaatg accttacgcg gttgcaaaca ggcgaggaat gctgctgatg cattaagcct
    11521 ttctggactc aggcagagat ttgtaacaaa ggaaacgaac tgcactaatt ttcaccgtag
    11581 cagatgattt ttgcgccttg tcgctgctgc gtgtggttgg taaagtaagc ggattttctt
    11641 ttccgcccca gctttcagga ttatccctta gtatgttgaa aaaatttcgt ggcatgtttt
    11701 ccaatgactt gtccattgac ctgggtactg cgaataccct catttatgta aaaggacaag
    11761 gcatcgtatt gaatgagcct tccgtggtgg ccattcgtca ggatcgtgcc ggttcaccga
    11821 aaagcgtagc tgcagtaggt catgacgcga agcagatgct gggccgtacg ccgggcaata
    11881 ttgctgccat tcgcccaatg aaagacggcg ttatcgccga cttcttcgtg actgaaaaaa
    11941 tgctccagca cttcatcaaa caagtgcaca gcaacagctt tatgcgtcca agcccgcgcg
    12001 ttctggtttg tgtaccggtt ggcgcgaccc aggttgaacg ccgcgcaatt cgtgaatccg
    12061 cgcagggcgc tggtgcccgt gaagtcttcc tgattgaaga accgatggct gccgcaattg
    12121 gtgctggcct gccggtttct gaagcgaccg gttctatggt ggttgatatc ggtggtggta
    12181 ccactgaagt tgctgttatc tccttgaacg gcgtggttta ctcctcttcc gtgcgcattg
    12241 gtggtgatcg tttcgacgaa gctatcatca actatgtgcg tcgtaattac ggttctctga
    12301 tcggtgaagc caccgcagaa cgtatcaagc acgaaatcgg ttcggcttac ccgggcgatg
    12361 aagtccgtga aatcgaagtt cgtggccgta acctggcaga aggtgttcca cgcggtttta
    12421 ccctgaactc caacgaaatc ctcgaagcac tgcaggaacc gctgaccggt attgtgagcg
    12481 cggtaatggt tgcactggaa cagtgcccgc cggaactggc ttccgacatc tccgagcgcg
    12541 gcatggtgct caccggtggt ggcgcactgc tgcgtaacct tgaccgtttg ttaatggaag
    12601 aaaccggcat tccagtcgtt gttgctgaag acccgctgac ctgtgtggcg cgcggtggcg
    12661 gcaaagcgct ggaaatgatc gacatgcacg gcggcgacct gttcagcgaa gagtaatcgg
    12721 atgcaggcag gggaagcgtc tgtttaccct gcctggtctg atacgagaat acgcataact
    12781 tatgaagcca atttttagcc gtggcccgtc gctacagatt cgccttattc tggcggtgct
    12841 ggtggcgctc ggcattatta ttgccgacag ccgcctgggg acgttcagtc aaatccgtac
    12901 ttatatggat accgccgtca gtcctttcta ctttgtttcc aatgctcctc gtgaattgct
    12961 ggatggcata tcgcagacgc tggcctcgcg tgaccaacta gaacttgaaa accgggcgtt
    13021 acgtcaggaa ctgttgctga aaaacagtga actgctgatg cttggacaat acaaacagga
    13081 gaacgcgcgt ctgcgcgagc tgctgggttc cccgctgcgt caggatgagc agaaaatggt
    13141 gactcaggtt atctccacgg ttaacgatcc ttatagcgat caagttgtta tcgataaagg
    13201 tagcgttaat ggcgtttatg aaggccagcc ggtcatcagc gacaaaggtg ttgttggtca
    13261 ggtggtggcc gtcgctaaac tgaccagtcg cgtgctgctg atttgtgatg cgacccacgc
    13321 gctgccaatc caggtgctgc gcaacgatat ccgcgtaatt gcagccggta acggttgtac
    13381 ggatgatttg cagcttgagc atctgccagc gaatacggat attcgtgttg gtgatgtgct
    13441 ggtgacctcc ggtctgggcg gtcgtttccc ggaaggctat ccggtcgcgg ttgtctcttc
    13501 cgtaaaactc gatacccagc gcgcttatac tgtgatccag gcgcgtccga ctgcaggact
    13561 gcaacgtttg cgttatctgc tgctgctgtg gggcgcggat cgtaacggcg ctaacccgat
    13621 gacgccggaa gaggtgcatc gtgttgctaa tgaacgtctg atgcagatga tgccgcaggt
    13681 attgccttcg ccagacgcga tggggccaaa gttacctgaa ccggcaacgg ggatcgctca
    13741 gccgactccg cagcaaccgg cgacaggaaa tgcagctact gcgcctgttg cgccgacaca
    13801 gcccgctgct aatcgctctc cacaaagggc tacgccgccg caaagtggtg ctcaaccgcc
    13861 tgcgcgtgcg ccgggagggc aatagtggcg agctatcgta gccagggacg ctgggtaatc
    13921 tggctctctt tcctcattgc gctgttgctg caaatcatgc cctggccgga taacctgatt
    13981 gttttccggc caaactgggt gttacttatc ttgttgtatt ggatcctggc cttgccccat
    14041 cgcgtaaatg tgggcacagg ttttgtgatg ggtgccatac tggatctgat cagcggctcg
    14101 atgcttggcg tacgcgtatt ggcgatgagc atcattgctt acctggtggc gctgaaatac
    14161 cagcttttcc gcaacctcgc attatggcag caggcgctgg tcgtcatgtt gctttcgctg
    14221 gtggtggata ttattgtttt ctgggcagag tttttagtga ttaacgtctc tttcagaccg
    14281 gaagtgttct ggagtagtgt agtcaatggg gtgctctggc cgtggatttt cttgctgatg
    14341 cgtaaagtcc gtcagcagtt tgcagtgcaa taaaggtttc tatgacttct ctgtatttag
    14401 cttccggttc tccgcgtcgt caggagttac ttgcgcaact tggcgtgacc tttgaacgta
    14461 ttgttacggg cattgaggag cagcgtcagc cgcaggagag cgcgcagcag tatgttgtgc
    14521 gtctggcacg cgagaaagca caggcgggtg tcgcgcaaac ggcacaggat ctcccggtgc
    14581 tgggtgcgga tattatcgtt atcctgaacg gagaagtgct cgagaaaccg cgcgacgcag
    14641 agcatgcggc gcagatgttg cgcaaattat cgggtcagac ccatcaggtg atgacggcag
    14701 tggcgttggc cgacagccag cacattctcg attgcctggt ggtcaccgat gtgactttca
    14761 gaacgttaac agacgaagac atcgcgggct atgtcgccag cggtgaaccg ttagataaag
    14821 caggtgcata cggtattcag gggctgggtg gctgttttgt caggaagata aatggcagct
    14881 atcacgccgt agtcggctta ccgctggttg aaacgtatga attattaagt aattttaacg
    14941 cactgcgtga gaaaagggat aaacatgacg gctgaattgt tagtaaacgt aacgccttcg
    15001 gaaacgcgag tggcgtatat tgatggcggt attctgcagg aaattcatat tgaacgtgag
    15061 gcgcgacgcg gaatagtagg caatatctac aagggtcgtg taagtcgtgt acttccgggt
    15121 atgcaggcgg cttttgtaga tattgggctg gataaagccg cgtttcttca tgcatccgac
    15181 atcatgccgc ataccgaatg tgtggcgggt gaagaacaaa agcaattcac ggtgcgcgac
    15241 atctcggaac tggttcgtca ggggcaagat ctgatggtgc aggtggtgaa agatccgctt
    15301 ggcactaaag gtgcgcgcct gaccaccgat atcacgctgc cttctcgcta tctggtattt
    15361 atgccagggg cttctcacgt tggggtttcc caacgaattg aaagcgaatc agaacgtgaa
    15421 cgtctgaaaa aagtggtcgc agagtattgc gacgagcagg gcgggtttat catccgcacc
    15481 gcagcggaag gggttggcga ggctgaactg gcctccgatg ccgcttatct gaaacgcgtc
    15541 tggaccaaag ttatggagcg taaaaagcgc ccgcagaccc gttatcagct gtacggcgaa
    15601 ctggcgctgg cgcagcgtgt tctgcgtgat tttgccgacg ccgaactgga ccgcattcgc
    15661 gttgactcac gcctgactta cgaagcattg ctggagttta cctcggagta cattcccgag
    15721 atgaccagca agctggagca ttacaccgga cgccagccga ttttcgatct ctttgatgtc
    15781 gaaaacgaaa tccagcgagc gttggaacgc aaagtagaac tgaaatccgg tggttatctg
    15841 attatcgacc agaccgaagc gatgaccacc gtggacatca ataccggagc gtttgtcggt
    15901 catcgcaatc tggacgacac tattttcaat accaatattg aagcgacgca ggctatcgct
    15961 cgccagttac ggttgcgtaa tctgggcggg attatcatta ttgatttcat cgatatgaat
    16021 aatgaagatc accgccgccg cgtgctgcat tcgctggagc aggcgttgag caaagaccgg
    16081 gtgaaaacca gcgttaatgg tttttcggcg ctggggctgg tggagatgac gcgtaaacgc
    16141 acccgcgaaa gcattgagca cgtactgtgt aacgaatgcc caacctgcca cggtcgcgga
    16201 acggtgaaaa ccgtggaaac ggtatgctat gaaatcatgc gcgagattgt tcgtgtccac
    16261 catgcttacg actccgaccg tttcctggtc tatgcttctc cggcagtagc cgaagccttg
    16321 aaaggcgaag agtcacactc gctggcggaa gtggaaattt tcgttggcaa acaggttaaa
    16381 gtacaaattg aaccgctcta taaccaggag cagtttgacg tcgtaatgat gtaaacagat
    16441 gctgggccgc catccggcaa agggtttttg agtcacattt ttagcagaca aggagtgacg
    16501 ggtgaggcga ttgccgggga ttttactgct tactggagcc gcgctcgttg tgatcgctgc
    16561 cctgctggtt agcggcctgc gtattgcttt accgcatctt aacgcctggc gtccggaaat
    16621 cctcaacaaa atagaatccg cgactggcat gccggtagaa gccagtcagc tctcagccag
    16681 ctggcagaat tttggcccga cgcttgaagc acacgacatc cgtgcagaac taaaagatgg
    16741 cggcgaattt tcggttaaac gcgttactct ggcgctggat gtctggcaga gcctgttaca
    16801 tatgcgctgg cagtttcgcg acctcacttt ctggcagctg cgctttcgca ccaacactcc
    16861 tatcaccagc ggtggtagcg atgacagtct ggaagccagt cacatcagcg atctgtttct
    16921 tcgtcaattt gaccatttcg atctccgcga cagtgaagtc agttttctga cgccatccgg
    16981 tcagcgcgcc gagctggcga tcccacaact cacctggctg aacgatccac gtcgacaccg
    17041 tgcggaaggc ctggtaagcc tctccagcct taccggacag cacggcgtga tgcaggtgcg
    17101 catggatttg cgcgatgatg aggggttgtt aagcaatggt cgcgtctggc tacaggcgga
    17161 tgacatcgac ctgaagccgt ggctcggtaa atggatgcag gacaatattg ctctggaaac
    17221 ggcacagttc tcccttgaag gctggatgac gatcgacaaa ggcgatgtaa ccggcggtga
    17281 cgtctggctg aaacagggcg gtgccagctg gttgggcgag aagcaaacgc atacgctgtc
    17341 ggtggataat ctgaccgcgc atattacgcg tgaaaatccg ggctggcagt tctctattcc
    17401 cgatacacgg atcacgatgg acggcaaacc ctggccgagc ggagcattga cgctggcctg
    17461 gataccggaa caggacgttg gcggcaaaga caataaacgc agtgacgaac tccggattcg
    17521 cgctagtaat ctggagctgg caggcctgga gggcatacgc ccgctggccg cgaaactttc
    17581 acctgcactg ggtgatgttt ggcgctccac acaaccgagc ggcaagatta acactctggc
    17641 gctggatatc ccgcttcagg cggcagacaa gacccgtttt caggcatcgt ggagcgatct
    17701 ggcctggaag caatggaaat tattaccggg tgcggaacac ttctccggga cgctttccgg
    17761 cagcgttgaa aatggtttgc ttaccgcgtc gatgaagcag gcaaagatgc cttacgaaac
    17821 ggtattccgt gcgccactgg aaatcgccga cggccaggca actataagct ggctgaacaa
    17881 tgacaaaggt ttccagctgg atgggcgtaa tattgacgtt aaagccaaag ccgtccatgc
    17941 gcgcggcggt tttcgttacc tgcaacctgc taacgatgaa ccctggctgg gtattctggc
    18001 tggcatcagt accgatgatg gttcacaagc ctggcgctat ttcccggaaa acttgatggg
    18061 taaagacctg gttgattatt taagtggtgc gattcagggc ggtgaagcgg ataacgcgac
    18121 gctggtttat ggtggcaatc cgcaactctt cccctataaa cacaacgaag gtcagtttga
    18181 agtgctggtg ccgctgcgca acgcgaagtt tgccttccag ccggactggc ctgcattaac
    18241 taaccttgat attgaactgg actttattaa cgacggttta tggatgaaaa ccgatggcgt
    18301 taatctgggc ggcgtgcgcg cgagtaatct caccgcagtg atccctgact actcaaaaga
    18361 aaaactgctg attgacgctg acattaaagg tccgggtaaa gccgttggcc cttactttga
    18421 tgagacaccg ctgaaagatt ctctgggtgc gaccctgcaa gaactccagc tcgacggcga
    18481 tgtgaatgct cgcttacatc ttgatatccc gctgaacggc gaactggtaa ccgcgaaagg
    18541 tgaagtgacg ctgcgtaata acagtctgtt tatcaaacca ctcgacagca tcctgaaaaa
    18601 tttgagcggt aaattcagct ttatcaatgg cgatctgcaa agtgaaccac tgacagcaag
    18661 ctggtttaat cagccgttga acgtggattt ttccaccaaa gaaggggcaa aagcctacca
    18721 ggttgcggtg aatctcaacg gtaactggca accggcgaaa accggcgttc tgcctgaagc
    18781 ggtgaacgaa gcattgagtg gcagcgtggc gtgggatggt aaagtgggca ttgatctgcc
    18841 ttatcatgct ggcgcgacct ataacgtaga gctgaacggc gatctgaata atgtgagtag
    18901 tcacttacct tcaccgttag ccaaacctgc gggtgaacca ctagcggtaa acgttaaggt
    18961 tgatggcaat ctcaacagct ttgaattaac cggacaggct ggtgcggata accatttcaa
    19021 tagccgctgg ttgcttggtc aaaagctgac gctcgatcgt gctatttggg cggcagacag
    19081 taaaacgctc ccgccgttgc cggaacaaag tggcgttgaa cttaatatgc cgccgatgaa
    19141 tggtgccgag tggctggccc tgttccagaa aggcgctgcg gagagtgtcg gtggtgcagc
    19201 gagtttccca caacacataa cgttacgtac gcctatgttg tcactgggaa atcagcaatg
    19261 gaataacctg agtattgttt cgcaaccgac ggcaaatggc accctggttg aggcgcaagg
    19321 gcgtgaaatc aacgccacgc tagcgatgcg taataacgcg ccgtggctgg cgaatatcaa
    19381 atatctttat tacaacccga gcgtggcgaa aactcgtggt gattcaacgc cgtcatcacc
    19441 tttcccgaca acggagcgca ttaacttccg tggctggccg gacgcacaaa tacgatgcac
    19501 agagtgctgg ttctgggggc aaaaattcgg tcgcattgac agtgatatca ccatttctgg
    19561 cgatacgtta acgctgacca atggactgat tgatactggt ttctcgcggc ttactgccga
    19621 tggtgaatgg gttaataatc cggggaatga acgtacctcg ctgaaaggaa aactgcgtgg
    19681 gcagaaaatt gatgccgccg cagaattttt tggtgtcacg acgcccatac gccagtcgtc
    19741 atttaatgtg gattacgatt tacactggcg caaagcaccc tggcagccag atgaagcgac
    19801 gttgaatggc atcattcata ctcaactggg taaaggcgaa attaccgaaa tcaataccgg
    19861 acatgccggg caattgctgc gcttattgag cgtagatgcc ctgatgcgta agctgcgttt
    19921 tgatttcaga gacacttttg gcgaagggtt ctattttgac tccattcgca gcaccgcgtg
    19981 gattaaagac ggcgttatgc acaccgacga cacgctggtg gatggcctgg aggcggatat
    20041 cgccatgaaa gggtcggtaa atctggtacg ccgcgacctg aatatggaag cggttgtcgc
    20101 accagagatt tctgcgacgg tgggcgtggc tgcggctttt gcggttaacc ccattgttgg
    20161 cgcggcagtg tttgccgcca gtaaagtgct ggggccgctg tggagcaaag tctccatttt
    20221 gcgctatcac atttcgggtc cgctggacga tccgcaaatc aacgaagtgt tgcgccaacc
    20281 gcgtaaagaa aaagcgcaat gatttgacga gggcgcgtaa ttgccccaat ctcatatgat
    20341 aatcgttgcc aaaggccaac gagccggaac ataactgtag gccggatagg gcgttcacgc
    20401 cgcatccggc agccgtaaaa aatcctctac tgcagtaact aacgagtagc aaaaacgatg
    20461 agtctgaacc tggtaagtga acaattgcta gcggcgaacg gcctgaaaca tcaggacttg
    20521 ttcgcgatcc tcggtcaact ggccgaacgt cgccttgatt atggcgatct ctattttcag
    20581 tcgagctatc acgaatcctg ggttttagaa gaccgcatta ttaaagatgg ttcttacaac
    20641 atcgatcagg gcgttggcgt gcgtgcaatc agcggtgaaa aaaccggatt tgcttacgct
    20701 gaccaaatca gcctgctggc gctggaacag agtgcgcaag cggcgcgcac catcgtccgt
    20761 gatagtggtg atggcaaagt acagacgctg ggcgcggtag agcatagccc gttgtatacc
    20821 tcggtagatc cgctgcaaag catgagccgt gaagagaagc tggatatcct gcgtcgcgtc
    20881 gataaggttg ctcgcgaagc ggacaagcgc gtacaggaag tgactgccag cctcagtggt
    20941 gtctatgaat taattctggt tgcggccacc gacggcacgc tagcggcgga tgtccgtccg
    21001 ctggtgcgtc tttccgtgag cgttctggtc gaagaagatg gcaaacgcga acgcggtgcc
    21061 agtggcggcg gcggtcgttt tggttatgag ttcttccttg ccgatcttga cggcgaagtc
    21121 cgtgcggatg catgggcaaa agaagcagtg cgtatggcgc tggtcaatct ttctgccgtt
    21181 gctgcaccag cgggcaccat gccggtagta cttggcgcag gttggccggg cgtgctgttg
    21241 catgaagcgg taggtcacgg tctggaaggc gacttcaacc gccgtggcac ttcagtattt
    21301 agtggacagg tcggggagct ggtggcttca gaactgtgta ccgtggttga tgacggcacg
    21361 atggtcgatc gccgtggttc ggtggcgatt gatgatgaag gtacgccagg ccagtacaac
    21421 gtgctgattg agaacggcat tctgaaaggc tacatgcagg ataaactcaa cgcgcgtttg
    21481 atggggatga cgccgactgg caacggtcgc cgtgaatcct acgcccatct gcccatgccg
    21541 cgtatgacca acacctatat gctgccgggt aaatcgaccc cgcaagaaat tattgaatcc
    21601 gttgagtacg gtatctatgc accgaacttt ggtggcggtc aggtggatat cacctccggc
    21661 aaattcgttt tctccacttc agaagcatat ctgattgaaa acggtaaagt aacgaagccg
    21721 gtgaaaggcg caacgttgat tggttccggt atcgaaacca tgcagcagat ttcgatggtt
    21781 ggcaacgacc tgaaactgga taacggcgtg ggtgtctgcg gtaaagaagg gcaaagtttg
    21841 ccggttggcg tgggccagcc aacgttgaaa gtcgataacc tgactgttgg cggtactgcg
    21901 taataatttc attattttca gaaggataat taatctttct acgtgcacga acggtcccct
    21961 cgctcctctg gggttagggt gaggggaacc cgttggcaca ggtttgtaga acgtaacagt
    22021 acaatatgaa ttacttctct ttcccgcgcc cgtgcatctc ctgaaacaat ttaccgacct
    22081 caacaaaata atccgtcagc gagttgatca cgacctgtac cttcagcggc agcttatctt
    22141 tttcggtata taacgcataa accgggcgtg gatctgactg ataacgcggc agcaggatct
    22201 ccagctcccc acgattgatc tcgttgatca cccacatcag cggcacgtag gcgatcccgg
    22261 cacccgccgt tagccagcgc accagcgtca tcggatcatt agtcacaaat ctcccttgcg
    22321 ggatcaggcg agtcgagatc ccttccggtg caatcagttc aaattcattg tcgggccgca
    22381 cgctgtattc aagccatgaa tggctactta aatcggcggg tttttccggt atgccgtatt
    22441 gtgtgagata gcttttcgcg gcgcacacca ccattggcat cgcgccaaga cggcgggaaa
    22501 acaggctgga atcctgcaac gcgccgacgc ggatcaccac atccagaccg tcggcaatca
    22561 ggtcgggggc tggaattccg gtaaccagat tggcgctcaa acctgggtat tctttcagca
    22621 ttttggctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag ccaatgcgta
    22681 gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc tgcacttcat
    22741 gaagcatacg acggcagccc tggtagtaaa ttctaccggc ttcggtcagg ccaatgctgc
    22801 gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac accgtctgac
    22861 tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag ccaaattcaa
    22921 ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct gacttaaaag
    22981 tgatttagat cacataatat agataacagc ataacagtta cgctaatata ttaaatatca
    23041 atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc gacagatgct
    23101 gaaaataata acgcctgctc tctctataca accaaggtca acatgagtct gtttcccgtt
    23161 atcgtggtgt ttgggctgtc cttcccaccg atattttttg aattgctttt atcactggcg
    23221 attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt tgtctggcat
    23281 ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc gcgactgttc
    23341 gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca tcacggtcgt
    23401 attagtcatt ctggccttca tcgcaatttt taatgcctgg gtctattaca ccgaatcccc
    23461 ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg acgtttctgg
    23521 actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac agatactgtt
    23581 caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta
    23641 ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt
    23701 gcaggcgatg tctcgcgaag agatcgacca ggctaacaac gtactacaaa cggttctgca
    23761 tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg aacgcacggt
    23821 gatccgagcg ccagcagatg gctgggtgac caacctcaac gtctataccg gtgagtttat
    23881 tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg tactggccta
    23941 tatggaagaa actaagctgg aaggggtgcg tccggggtat cgtgcagaga tcacgccgct
    24001 tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgctgggg tcaccaacgc
    24061 cagcagcacg cgtgacgaca aagggatggc gaccatcgac tctgaccttg aatgggtgcg
    24121 tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga acatctggcc
    24181 tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg aaagccagga
    24241 ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac gatgggtatt
    24301 ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc cattgtactg
    24361 gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt actgacagcg
    24421 gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc tggcgctatt
    24481 cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc cggactggtg
    24541 atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg tatctgggcc
    24601 ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc gtgggggctg
    24661 gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt gcttacgccg
    24721 cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc gattatggcg
    24781 gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct ggaaagtttg
    24841 ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc
    24901 gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat gcgcagcaac
    24961 ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg
    25021 ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg
    25081 gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac
    25141 gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggactgggga acgggaaacg
    25201 cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc
    25261 gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg cgaaccggaa
    25321 gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg aaccacactt
    25381 tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg cagtggtgca
    25441 atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg
    25501 gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct ctactttttg
    25561 gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct ggcagtgctg
    25621 ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg ggcactggcc
    25681 agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag tcagtttctc
    25741 gacagcgcat taggacaaat cgtcggctgt gtgctcgcgt tcaccgttat tttgctggtg
    25801 cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt ttctgccgct
    25861 gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc ggcactttat
    25921 cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt tcgcctggcg
    25981 ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat
    26041 ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc tgcccgtagc
    26101 gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat ctaccaggaa
    26161 aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg gctggcgggg
    26221 atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga cgccaaaagc
    26281 gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg aaacgtgacg
    26341 gtggcaacag atgaatattt atgcctttga ttttgatgag attgagagtc aggaggattt
    26401 ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg atctcgactc
    26461 actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg aatttgttca
    26521 tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg atgaggcaga
    26581 ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa aaaagcccac
    26641 gaaccggggg caatatcgtc ggacaaaacg atgagggttt atttgtacag ttcagccgta
    26701 gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc gcctttctct
    26761 tccgcttttt tgttcagcat ttcacgtata tccattggcg aagacgccac accacttacg
    26821 gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga gtcggcagcg
    26881 aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt tttgattttc
    26941 atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc catatcacaa
    27001 aatcaagtat acactaatta ttgaactaat taataccaca ctaactgttt cagttgatac
    27061 taagtgttaa aaatgtgaat tttaataaca aaaaagtata aaaaacacgg ttatttatta
    27121 ttaattatag tattttcaat tagataatga attttgcata atgtgctttt tttaccgtat
    27181 gtattcattt tgtgaatgat gtgtcgcggt aaaacgcact attcgttaaa tgtatcgcag
    27241 gagaaagaag gggaatgaga gggataagca acattttccc cgccgccaga agcgacgggg
    27301 cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt ctttgacggt
    27361 gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc ccagaatacc
    27421 ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc cagggctggt
    27481 atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct tcaatggact
    27541 ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca ttttggcatt
    27601 gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact gattaatatt
    27661 gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa atttctcttc
    27721 tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc ttcgcataag
    27781 tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt ttatgcaaac
    27841 agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta tcaaatttcg
    27901 ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta aattaattgt
    27961 tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc tgttggaatg
    28021 ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt acggcaatta
    28081 aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca ttgtaaacgg
    28141 caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat caatataata
    28201 aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc caggcgcttg
    28261 cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat gatatcgctc
    28321 cagtgactcc cggtgtggcc gtcgatctga gccatatccc tactgctgtg aaaatcaaag
    28381 gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt cttatctctg
    28441 caggcgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt aacgccggca
    28501 tcgtgaaaaa cctggtacag caagttgcga aaaactgccc gaaagcgtgc attggtatta
    28561 tcactaaccc ggttaacacc acagttgcaa ttgctgctga agtgctgaaa aaagccggtg
    28621 tttatgacaa aaacaaactg ttcggcgtta ccacgctgga tatcattcgt tccaacacct
    28681 ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt attggcggtc
    28741 actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt tttaccgagc
    28801 aggaagtggc tgatctgacc aaacgtatcc agaacgcggg tactgaggtg gttgaagcga
    28861 aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt tttggtctgt
    28921 ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac gttgaaggcg
    28981 acggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac ggcgtggaag
    29041 agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa ggtatgctgg
    29101 atacgctcaa gaaagatatc gccctgggca aagagttcgt taataagtaa ttgattagcg
    29161 aataataaaa aaccggagca cagactccgg tttttgtttt gagcactcga cttaattggt
    29221 tgccggatat tcctgaatgg tgacctgcag cgttaactgc ttatcatcac gcatcactac
    29281 aaccgggatc accgaaccag ggcgaatttc cgccacctga tccatcgtct ccagagcaga
    29341 gatggccggt ttgttatcca ccgaaataat cagatcgttg acctgaatac ccgcattcgc
    29401 cgctgggccg tcaggtgaca cttcattaac cacgatccct tgcagttgat ctataccacc
    29461 gctctgcgcg tgcagtggtg cgatctcgcg tccgccgata ccaatgtagc cgcggatcac
    29521 gcgaccatcg cggatcagct tatccataat tttggttgct aactggaaag gaatcgcaaa
    29581 gccgatacct tccggcgttt cgccatcgtt actcttatca aacgacagcg tgttaatgcc
    29641 catcagttct cccagcgagt tcaccagcgc gccgccagag ttaccgtggt taatggaagc
    29701 atcggtttgt aggaagtttt gccgcccggt cgggttcaga ccgattcgac ccgtggcact
    29761 aataatccct tgggtaatgg tctgcccgag gttgtacggg ttaccgatcg ccagtactac
    29821 gtcgccaatg tgcggtacgc gacgtgcatt aattggaatg gtaggtaaac cgccagtggc
    29881 attaatttta agtaccgcca gatcggttag agagtcagat cccaccagca atgcttcaaa
    29941 tacacgtcca tcctgtaagg cgacgatgat ctgatcggcg tcgttgatga cgtgtttatt
    30001 ggtgatgata taaccgcgtt gatccatgat tacaccggat cccagggtgc ggatctcaag
    30061 ctggttgtga gagttggtgt tcaaaccacg gttgtaaacg ttaaccaccg ctggcgcggc
    30121 gcggcgaacc gccagattat agctggcagg cgtctcatcg gtactgtcaa attgcggagt
    30181 ggaaagcggg ttaaggctgc gcagcgaagg catggcaacc agcagaatag cgccgacaat
    30241 taatccaatc gcaacggaac gtaagagctt cacaaacatg atggaggcgt cattaaaaaa
    30301 gggaacggca gcagcatacc acgagttaac cggacatcac acgtcagcct gatgcccggt
    30361 ttacgacatt aacgcatcag cagatagatg ctttcattgc cgcgtacaat ttgcagggcg
    30421 atgatggccg gttttgccgc cagcacttta cgcatttcag caatcgagtt cacccgatcg
    30481 cggttgacgc caatgatcac atcgtctttt tgcaagccag cctgagcagc tgggcttcct
    30541 ttgacaactt catctatctt aatacctttg ccgccatctt ttagctgacc atcgctcaac
    30601 gttgcacctt ccagcgctgg ccgacgaaga ggtgctggta tcaagcgtca cttctacttc
    30661 cagtggtttg ccgttacgca gcaggccaag cttcactttc gtgcccggct ctgtagtcgc
    30721 gatacgagag cgcaactcag caaagctatt cagcggtttg ccgttgaggc tggtaataat
    30781 atcgcccgct ttgacgcccg ctttcgctga gccagaactt ggcaacactt cgctgacaaa
    30841 cgcgccacgc tgcacgtcaa ggttgaaggc tttggcgata tcggcactca tctcggtgcc
    30901 tttgatgcct aacaaaccgc gtttgatttc accaaagtcg ataagctgct gcgccagtgt
    30961 tcgcgccata ttactgggga tggcaaatcc aatcccgacg ctcccgccgc caggcgcaag
    31021 gattgcagtg ttgatgccaa ttaactcacc gttaaggttt aatagtgcac cgccggagtt
    31081 accgcggtta atggaagcat ctgtctggat aaagttttcc agaccttcaa gattcaaccc
    31141 gctgcggcct aatgcggaaa caatgccaga ggtggcggtt tgcccaaggc caaatgggtt
    31201 accgaccgct acggcaaaat caccgacgcg caatttatcg gagtcggcaa tagcgatttg
    31261 cgttaatttg ctcgggtttt gaatttgtaa cagggcgata tcgctctggt catcgctacc
    31321 aatcagtttt gcatcaaact cgcgcccatc attgagctga atactgattt tctgtgcctg
    31381 attaatcaca tggttgttgg tcagcacata gcctttactg gcgttgatga tgacaccgga
    31441 acctaaacct tcgaagggtt gtgcaggttg atccggtaaa tcatcaccaa aaaacttttt
    31501 gaattcttcc gggattttct gtccctgact ggccgttcct tccacccgta cgctcaccac
    31561 tgccggaagc actttttcca gcattggagc cagactgggg agaggggcct gatcggcaac
    31621 ctggcctgga atcgacgcga cggcctgaaa tgacgccgag agagttaacc cggcacttaa
    31681 cgctaatgca ctcaacagct gggtttgttt tttcattatt cctgctctcg tacctgaatg
    31741 ataagaaaag agattcaaaa cagtttgatg ttattgaatt ttcagacggc taacaatgag
    31801 acaccagatt aaatgatagt cggggaggga gagaagagga gggcgcaatg gctgcgcccg
    31861 aaaaataaat tagtcgcgct tcgcgccagt acgcagcagg ccggatgcgc cttcagaata
    31921 gtcgcgaggc acctgcaccg gtgcctgatc gttgctggct tcagactctg ccagacgatt
    31981 acggaacggg tttgcttcag cagacagttc cggcagcagg ctgctggagc tttttgccat
    32041 gtgctgatac agctggcgat agtcgtgcgc catggtatcc agtaattccg cgctgcgggc
    32101 aaagtggcta accagctctt cgcgatactc gtccagttca gctttattct tttccagttc
    32161 gtactgcaac gcctgttgct ggcgtagttt acgattacca aaacgcatgg ccacagcacc
    32221 aataatgatg ccgacgacta acccaattag cgcatattcc caggtcatga acatctcccg
    32281 ttgtcttttg tttccgtagg gtgttggctt caggctccat gcctgcggct gattatgcca
    32341 ctataaccgt taattccaca gaagtggaat cccgactgca tatcgcgtag tgtagaacgg
    32401 cctttttttc gtcaaccgtg aacaacggcg caccgattat tcaaggaata acaataagat
    32461 catgcaaagc gttaccccaa catcgcaata cctgaaggcg ctcaatgaag gcagccatca
    32521 acccgacgac gttcaaaaag aggccgtcag ccgcctggaa attatttatc aagaactcat
    32581 caatagcagg ccaccagccc ccaggacgag tgggctaatg gcgcgggtcg gtaagctgtg
    32641 gggtaaacgc gaagacacaa agcatacgcc agtgcgtggc ttatatatgt ggggcggtgt
    32701 aggacgcggg aaaacctggc tgatggacct tttctatcaa agcctgccgg gagagcggaa
    32761 acagcgcctg cactttcacc gttttatgct gcgggtgcac gaagagctaa ctgccttaca
    32821 ggggcagacc gatccgctgg aaattattgc cgatcgcttt aaagccgaaa ctgacgtgct
    32881 ctgttttgac gaattttttg tttctgatat taccgacgcc atgctacttg gcggtctgat
    32941 gaaagccctg ttcacccgcg gtattaccct ggtagcgacg tcaaatattc cgccggacga
    33001 actttatcga aatggcctgc aacgtgcgcg ttttctgcct gcaatcgatg ccattaaaca
    33061 gcattgtgat gtaatgaacg tggacgctgg tgttgattat cgactgcgta cactcactca
    33121 ggcgcatctg tggctttcgc cactcaacga tgaaacccgg gcgcagatgg ataaactatg
    33181 gttggcgctg gcgggggtga aacgagaaaa ttcaccgacg ttagaaatca accatcggcc
    33241 attggcgaca atgggcgtcg agaaccagac gctggcggtt tcttttacta cgctgtgcgt
    33301 cgacgcccgc agtcagcatg actatattgc gctctcacgc ctgtttcaca cagtaatgtt
    33361 gtttgatgtg ccagtcatga cgcggttgat ggagagcgaa gcgcggcgct ttattgcgct
    33421 ggtggatgag ttttacgagc gccatgtcaa attagtggtg agtgcagaag tgccgctgta
    33481 tgaaatttat cagggtgagc ggctgaagtt tgagttccag cgttgcctgt cacgtctgca
    33541 agagatgcaa agcgaagagt atctgaagcg cgagcatttg gcgggttaaa acctgccaca
    33601 aatcacaaaa aggggtcgat ctttgacccc gacttctcta taatcctgcg accccacgtt
    33661 acaagaaagt ttttttccca aaactttttg tgtgctggca taggctattc gaaggggtag
    33721 gtttgccgga ctttgtcgtg tgaacctcaa caattgaaga cgtttgggtg ttcaccaacg
    33781 tgtaactatt tattgggtaa gcttttaatg aaaactttta cagctaaacc agaaaccgta
    33841 aaacgcgact ggtatgttgt tgacgcgacc ggtaaaactc tgggccgtct ggctactgaa
    33901 ctggctcgtc gcctgcgcgg taagcacaaa gcggaatata ctccgcacgt agataccggt
    33961 gattacatca tcgttctgaa cgctgacaaa gttgctgtaa ccggcaacaa gcgtactgac
    34021 aaagtgtact atcaccacac cggccacatc ggtggtatca aacaagcgac ctttgaagag
    34081 atgattgctc gccgtcctga gcgtgtgatt gaaatcgcgg ttaaaggcat gttgccaaaa
    34141 ggcccgctgg gtcgtgctat gttccgtaaa ctgaaagttt acgcgggtaa cgagcacaac
    34201 cacgcggcac agcaaccgca agttcttgac atctaatcgg gattataggc aatggctgaa
    34261 aatcaatact acggcactgg tcgccgcaaa agttccgcag ctcgcgtttt catcaaaccg
    34321 ggcaacggta aaatcgtaat caaccaacgt tctctggaac agtacttcgg tcgtgaaact
    34381 gcccgcatgg tagttcgtca gccgctggaa ctggtcgaca tggttgagaa actggacctg
    34441 tacatcaccg ttaaaggtgg tggtatctct ggtcaggctg gtgcgatccg tcacggtatc
    34501 acccgcgctc tgatggaata cgacgagtcc ctgcgttctg aactgcgtaa agctggcttc
    34561 gttactcgtg acgctcgtca ggttgaacgt aagaaagtcg gtctgcgtaa agcacgtcgt
    34621 cgtccgcagt tctccaaacg ttaattggct tctgctacgg cagaaaacaa tttgcgaaaa
    34681 aacccgcttc ggcgggtttt tttatggcta aattctgaat cagcgtaaaa aatgggaagt
    34741 tgctttttgc tgccgtctga aagacaggta aaacaaaaac cacatcgcca ataagggtct
    34801 aagtcaacta tttcagacta aagcgcatct ctttttcccc atttccggca tccactcacc
    34861 acaaaggtcg caaaatctgg taaactatca tccaattttc tgcccaaatg tcgggtattg
    34921 ctcatttttt gtttgatttt cgaacaaaga gagtcgttcc ttattgggta acacaacttc
    34981 tgactggcca cctggtggct ggtagcagta aaaattctga ctatacctgg aggttttcat
    35041 ggctgtcgct gccaacaaac gttcggtaat gacgctgttt tccggtccta ctgacatcta
    35101 tagccatcag gtccgcattg tgctggctga gaaaggtgta agtttcgaga tcgaacacgt
    35161 ggaaaaggac aatccgcctc aggatctgat tgacctcaac ccgaatcaga gcgttccgac
    35221 cctggtggat cgtgagctga ccctgtggga atctcgcatc attatggaat atctggatga
    35281 gcgtttcccg catccgccac tgatgcctgt ttacccggta gctcgcggtg aaagccgtct
    35341 gtacatgcat cgcatcgaaa aagactggta cacgctgatg aacactatca tcaacggttc
    35401 agcttctgaa gcagatgccg cacgtaagca actgcgcgaa gaactgctgg cgattgcgcc
    35461 ggtcttcggt cagaagccgt acttcctgag cgatgagttc agcctggtcg attgctacct
    35521 tgctccgctg ctgtggcgtc tgccgcaact gggcatcgag ttcagcggcc cgggtgcgaa
    35581 agagctgaaa ggctatatga cccgcgtctt tgagcgtgac tctttccttg cttctttaac
    35641 tgaagcagaa cgtgaaatgc gtctgggccg gagttaatct gtatggattt gtcacagcta
    35701 acaccacgtc gtccctatct gctgcgtgca ttctatgagt ggttgctgga taaccagctc
    35761 acgccgcacc tggtggtgga tgtgacgctc cctggcgtgc aggttcctat ggaatatgcg
    35821 cgtgacgggc aaatcgtact caacattgcg ccgcgtgctg tcggcaatct ggaactggcg
    35881 aatgatgagg tgcgctttaa cgcgcgcttt ggtggcattc cgcgtcaggt ttctgtgccg
    35941 ctggctgccg tgctggctat ctacgcccgt gaaaatggcg caggcacgat gtttgagcct
    36001 gaagctgcct acgatgaaga taccagcatc atgaatgatg aagaggcatc ggcagacaac
    36061 gaaaccgtta tgtcggttat tgatggcgac aagccagatc acgatgatga cactcatcct
    36121 gacgatgaac ctccgcagcc accacgcggt ggtcgaccgg cattacgcgt tgtgaagtaa
    36181 tacaaaacag gcccaggcgg cctgttttgt ctttttaatg acttaataaa atagcgtgat
    36241 tgtggcaatc atattcacca cgaaaccgac tgccatgggg atcgctgtcc gcttcaccac
    36301 cgcaaaaggc gaaacgcctg caattcctga cgtaacgacc accaccgcag taatcggcga
    36361 aaccgcgcgc gctagcgtcg tggcaaaatg catcggcata atcattacaa ccgctggtac
    36421 atgtagtccg gctgcgatat tcggaataag actggcaaat ggcataaacg gcgcattgcc
    36481 agagcccatc acaatggcac aaatggcaat gactagcgcc ataataatca tcacgccaat
    36541 accgcccaga ccagaatgct ccgcacccct gataaccgca tcaacagtgc caatcgtcgt
    36601 taagcctttc gcaaatattt cacccgcgac caccagcgtt accacgttga caaactgcgt
    36661 acccatgccg tcaaaaaacg cctgcacatc gtccattgtt tcgcgcaagt tatgcttgcg
    36721 gaagaactca acaaacatcg tcaccgtcaa acttagtaac atcaccacca ccagatgcag
    36781 ttctgactgc atcagcccga cgtgggcgag gaacagcgag ccgagcatca ggattaacgg
    36841 catcacaggt aaaatggcgt aatagagcgg cgggacatta tcgagagttt tttgctctgc
    36901 ctgttcgtga ttgatatttt tatctttttt gtcaaaagcg cgttgcacga aaaagtggga
    36961 gatcgccacc gagataatga cgcaagaggc gaccggaagc tggtagtgga agaagtatgt
    37021 ggcaattttc attcccgcga cctgggctgc aaaaatggag ttcgtttcca gaatccccca
    37081 ctcaatggac atcgtggttg cgataaccgc caccgcagag aggcgactta ctcccagact
    37141 caccagcgtc ggaaataagg tgaccatcag caacataccc agaccggagg cgctggtaat
    37201 aaactgcgcc atgatttggc cgatgacgta agttgccgac agaataatat acggcgagcg
    37261 aatgagtttt aacgggcggc ttagcaagct caccatcgcg caactggctc ctatgcgctc
    37321 catgtagcgg gcataaccgc ccaccgccat aatcgacagc cccaatccgg caatgcggtt
    37381 ggacagcatg cgcaggattt cattataaat gtcgacgatc aagtagccgc tgcttttggc
    37441 gggatcgagt acaccaccga accctaacca gactccgcac atcatcagga agacacctgc
    37501 ggcagccagc accacctgag gtttgtagtt tttcaggatc aaatagccca tcgtaattaa
    37561 tacgatgaca gatataatta tgccgaacat t
//
LOCUS       DAJTNP010000030        36589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTNP010000030 DAJTNP010000000
VERSION     DAJTNP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(158..1393)
                     /locus_tag="OO160_001673"
     CDS             complement(158..1393)
                     /locus_tag="OO160_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5523838.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1540..1616)
                     /locus_tag="OO160_001674"
     tRNA            complement(1540..1616)
                     /locus_tag="OO160_001674"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1580..1582),aa:Pro,seq:tgg)
     gene            complement(1659..1745)
                     /locus_tag="OO160_001675"
     tRNA            complement(1659..1745)
                     /locus_tag="OO160_001675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1709..1711),aa:Leu,seq:cag)
     gene            complement(1766..1841)
                     /locus_tag="OO160_001676"
     tRNA            complement(1766..1841)
                     /locus_tag="OO160_001676"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1806..1808),aa:His,seq:gtg)
     gene            complement(1900..1976)
                     /locus_tag="OO160_001677"
     tRNA            complement(1900..1976)
                     /locus_tag="OO160_001677"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1940..1942),aa:Arg,seq:ccg)
     gene            complement(2079..3464)
                     /locus_tag="OO160_001678"
     CDS             complement(2079..3464)
                     /locus_tag="OO160_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5523839.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3655..4395)
                     /gene="rffM"
                     /locus_tag="OO160_001679"
     CDS             complement(3655..4395)
                     /gene="rffM"
                     /locus_tag="OO160_001679"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5523840.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4398..5750)
                     /gene="wzyE"
                     /locus_tag="OO160_001680"
     CDS             complement(4398..5750)
                     /gene="wzyE"
                     /locus_tag="OO160_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5523841.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5747..6826)
                     /gene="rffT"
                     /locus_tag="OO160_001681"
                     /gene_synonym="wecF"
     CDS             complement(5747..6826)
                     /gene="rffT"
                     /locus_tag="OO160_001681"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5523842.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6823..8073)
                     /gene="wzxE"
                     /locus_tag="OO160_001682"
     CDS             complement(6823..8073)
                     /gene="wzxE"
                     /locus_tag="OO160_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5523843.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8075..9205)
                     /gene="rffA"
                     /locus_tag="OO160_001683"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8075..9205)
                     /gene="rffA"
                     /locus_tag="OO160_001683"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5523844.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9210..9884)
                     /gene="rffC"
                     /locus_tag="OO160_001684"
                     /gene_synonym="wecD"
     CDS             complement(9210..9884)
                     /gene="rffC"
                     /locus_tag="OO160_001684"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5523845.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9862..10743)
                     /gene="rfbA"
                     /locus_tag="OO160_001685"
     CDS             complement(9862..10743)
                     /gene="rfbA"
                     /locus_tag="OO160_001685"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5523846.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10762..11829)
                     /gene="rffG"
                     /locus_tag="OO160_001686"
     CDS             complement(10762..11829)
                     /gene="rffG"
                     /locus_tag="OO160_001686"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5523847.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11829..13091)
                     /gene="wecC"
                     /locus_tag="OO160_001687"
     CDS             complement(11829..13091)
                     /gene="wecC"
                     /locus_tag="OO160_001687"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5523848.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13088..14218)
                     /gene="wecB"
                     /locus_tag="OO160_001688"
     CDS             complement(13088..14218)
                     /gene="wecB"
                     /locus_tag="OO160_001688"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5523849.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14274..15320)
                     /gene="wzzE"
                     /locus_tag="OO160_001689"
     CDS             complement(14274..15320)
                     /gene="wzzE"
                     /locus_tag="OO160_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5523850.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15332..16435)
                     /gene="wecA"
                     /locus_tag="OO160_001690"
     CDS             complement(15332..16435)
                     /gene="wecA"
                     /locus_tag="OO160_001690"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5523851.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16675..17934)
                     /gene="rho"
                     /locus_tag="OO160_001691"
     CDS             complement(16675..17934)
                     /gene="rho"
                     /locus_tag="OO160_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5523852.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18019..18120)
                     /gene="rhoL"
                     /locus_tag="OO160_001692"
     CDS             complement(18019..18120)
                     /gene="rhoL"
                     /locus_tag="OO160_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5523853.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18261..18590)
                     /gene="trxA"
                     /locus_tag="OO160_001693"
     CDS             complement(18261..18590)
                     /gene="trxA"
                     /locus_tag="OO160_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5523854.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18721..19986
                     /gene="rhlB"
                     /locus_tag="OO160_001694"
     CDS             18721..19986
                     /gene="rhlB"
                     /locus_tag="OO160_001694"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5523855.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20120..21604
                     /gene="gppA"
                     /locus_tag="OO160_001695"
     CDS             20120..21604
                     /gene="gppA"
                     /locus_tag="OO160_001695"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5523856.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21651..23672)
                     /gene="rep"
                     /locus_tag="OO160_001696"
     CDS             complement(21651..23672)
                     /gene="rep"
                     /locus_tag="OO160_001696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5523857.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23759..24040
                     /gene="ppiC"
                     /locus_tag="OO160_001697"
     CDS             23759..24040
                     /gene="ppiC"
                     /locus_tag="OO160_001697"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5523858.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24086..25561)
                     /gene="ilvC"
                     /locus_tag="OO160_001698"
     CDS             complement(24086..25561)
                     /gene="ilvC"
                     /locus_tag="OO160_001698"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5523859.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25711..26601
                     /gene="ilvY"
                     /locus_tag="OO160_001699"
     CDS             25711..26601
                     /gene="ilvY"
                     /locus_tag="OO160_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5523860.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26598..28142)
                     /gene="ilvA"
                     /locus_tag="OO160_001700"
     CDS             complement(26598..28142)
                     /gene="ilvA"
                     /locus_tag="OO160_001700"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5523861.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28145..29995)
                     /gene="ilvD"
                     /locus_tag="OO160_001701"
     CDS             complement(28145..29995)
                     /gene="ilvD"
                     /locus_tag="OO160_001701"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5523862.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30060..30989)
                     /gene="ilvE"
                     /locus_tag="OO160_001702"
     CDS             complement(30060..30989)
                     /gene="ilvE"
                     /locus_tag="OO160_001702"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5523863.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31009..31272)
                     /gene="ilvM"
                     /locus_tag="OO160_001703"
     CDS             complement(31009..31272)
                     /gene="ilvM"
                     /locus_tag="OO160_001703"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5523864.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31269..32915)
                     /locus_tag="OO160_001704"
     CDS             complement(31269..32915)
                     /locus_tag="OO160_001704"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5523865.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32918..32968)
                     /locus_tag="OO160_001705"
     CDS             complement(32918..32968)
                     /locus_tag="OO160_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523866.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33054..33152)
                     /gene="ilvL"
                     /locus_tag="OO160_001706"
     CDS             complement(33054..33152)
                     /gene="ilvL"
                     /locus_tag="OO160_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5523867.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33504..34983
                     /locus_tag="OO160_001707"
                     /pseudo
     CDS             33504..34983
                     /locus_tag="OO160_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35008..35346)
                     /gene="maoP"
                     /locus_tag="OO160_001708"
     CDS             complement(35008..35346)
                     /gene="maoP"
                     /locus_tag="OO160_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5523868.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35465..36304
                     /gene="hdfR"
                     /locus_tag="OO160_001709"
     CDS             35465..36304
                     /gene="hdfR"
                     /locus_tag="OO160_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5523869.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36400..36475)
                     /locus_tag="OO160_001710"
     tRNA            complement(36400..36475)
                     /locus_tag="OO160_001710"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36440..36442),aa:Trp,seq:cca)
     gene            complement(36484..36560)
                     /locus_tag="OO160_001711"
     tRNA            complement(36484..36560)
                     /locus_tag="OO160_001711"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36524..36526),aa:Asp,seq:gtc)
BASE COUNT         8942 a        10013 c         9215 g         8419 t
ORIGIN      
        1 ctgccaccgc acgtaaaggc tttgccggat acgatcaccc aaaccagtat ccacagaacg
       61 ctgcaattta ttgaatttgc acgatcatgt aggccggata aggcgtttac gccgcatccg
      121 gcaatcaacc gcaggcggct tccaaatggc cgctttttta cttattcacc accatcaaat
      181 gtgcctgcat aatgtcactg gccgggcgac cgtgcgccag caaatcagcc attgctttaa
      241 gatatggcgg tagatggcgg aaataacgct gatacccggc acacaaataa ttcagccccg
      301 gtttgccgct ggcatcgagc atgaagcggt gtttcgggca gcctccccaa catgctttta
      361 acacgttaca actgcgacac tgcgccggta attgcttaaa tttatcttca ccaaacacct
      421 gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg atgcatattc cccagccgat
      481 attgcggata aacatagtga tcgcaggcgt aaacgtcgcc gttgtgctca acaatcaccg
      541 agcgcccaca ggttggctga tgatggcaaa ccgcacccgg cgcaccgaca aaattggcaa
      601 acgcccattc gatattcatc acgaaaatct tgccgacgtc gcgtttgatc cagtggtcaa
      661 aaatcgccac cagaaattca ccaaattcat cggggcgcac agaccattcc gtcagttccc
      721 cctgaatatc accaggcgca tgtagtttca gtccatcgcg gacagctgtt tcatcagcca
      781 ggcgctcgac caccggaata aactggatga attcgactcc cgcatcgcac aaaaaatcat
      841 aaacctgtaa cggttgctgc gcgctggtgc ggttgacgca gaccagcacg ttatagtcga
      901 catgatgttt ttgcaggagc gtcagggcac gcatcaccag tttatgcgtg ggtctgccgc
      961 ctttggtcac gcgatattga ttgtggatct cagccggacc atccagcgat aacccaacaa
     1021 gaaaattatt ttctgccaga aatgcacacc attcgtcatc gagcagcacg ccgttagtct
     1081 ggaaactgtt acttatcttc ctgccagcac catatttcgc ctgtagcgcc acggcacggc
     1141 ggtaaaactc cagcccgagt agcgttggtt cgccgccctg ccaggtaaaa gccacttcgt
     1201 tttgcagttc gctggcggcg atatagtgac ggatatacgc ttccagcgtg tcatcgtcca
     1261 tatgcgtgac tggcttttcg cggtaaaggg attgtttttc gagataaaaa cagtagtcac
     1321 agttcagatt acaatcggaa ccgctcggtt tcgctatcac atgaaaagca cgcgttggaa
     1381 cctgttgcag catggtcgct ccttgcctga agtcatcctt caatacgtta agggcgaaat
     1441 cataccgtgg ggcaatgagt tattacgggc gtttattgcg aagggagggt gacgaaatgc
     1501 acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg
     1561 aacctccgac ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat
     1621 actgcttttt gaatttttag ttcaattctt taaagtcgtg gtgcgagggg ggggacttga
     1681 acccccacgt ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc
     1741 ttcgcattgt cacaacttct aataatgggg tggctaatgg gattcgaacc cacgacaact
     1801 ggaatcacaa tccagggctc taccaactga gctatagcca ccactacaaa tcttgttacg
     1861 cggtattact accaccgcag ctcaagcgct gggactaaat ggcgcgcccg acaggattcg
     1921 aacctgagac ctctgcctcc ggagggcagc gctctatcca gctgagctac gggcgcttag
     1981 cgccgttgcg ggggtggata atacggactt cccacccctc tgtctagtcc ctttttaaat
     2041 aaattgctcg tttggttacg gtttgtgcgt tttgctgctt attcctccag tttatgcgct
     2101 ttaccgtggc gattaagccc aaaaacttta taaatcgccg tcaccgccag cataaagatg
     2161 atgccgacaa acagcgacat acgcgtatct tcattaaagt acatgccgat caaaacgcaa
     2221 atcaggaatg ccattgttac gtagttggcc cacgggaaca ggattgagcg gaacggatgg
     2281 ctggcaatcg ccgctttatg tgcacgccga aaacgcagct ggcttatcaa tatcacaaac
     2341 catggcacca tccccggaag cacgctggca ctgtagacgt agacaaacac acgctgcgga
     2401 ttgggaatga tgtagttcag gcatgagcca attagcagaa tagcaataga tactgccaca
     2461 cccgcaaccg gtacgccgtg acgggaaact tttgccattg ccgccggtaa ctgacggttt
     2521 ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc cgctgttaca gccagagagc
     2581 gcagccgtca gcaccacaaa gttgataatg cccgccgctg cggtaatacc gattttggca
     2641 aaagtcagta cgaacgggct gccgttgctg ccaatttcat tccacgggaa gatggtgacg
     2701 ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc acagcacttt gcctacggca
     2761 ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag taatgccaat cagctccacg
     2821 ccctggtagg acgccaccac aatacacaga gcggtcagga accctttcca gccacccgca
     2881 aagaaaccgc catgctctgt gagattgcta aaaccaatcg actgcccgcc attgccaaag
     2941 ccaaagaaaa tcacgcccaa gccaatgaca atcatcacga taatcgtggt gactttgatc
     3001 atcgcgaacc agaactcgat ttcgccgtac aaccgtaccg ccgccagatt cgccaacgcc
     3061 accagcgcca ccgcgatcaa tgcgggtatc cactgcgcca tctccgggga ccaaaactgg
     3121 acataaacgc caatggcggt gatttctgag atccccaccg ccatccacat aaaccagtaa
     3181 gaccaggcgg tgagatagcc aaaaaacggg ctcatataac gatgcgcata aacggcgaac
     3241 gaaccggtaa ccggttcgag gaacaacatt tcgcccattg aacgcatgat gaaaaaaacg
     3301 aacagcccgg cgatgatata ggccaacaat acggatggcc cggcccattt cagggtactg
     3361 gcggccccca taaacaggcc gacgccaatg gtgcccccca gggcgatgag ttcgatatgt
     3421 cgagcttcca gcccacgctg tagctctggt ttgttatctg ccataaatcc tcgtgttgtg
     3481 tttgcatgct ttccggtgtt accggttatc gttatgggta catcgagtgt tgcgaatgtt
     3541 ttcgtaattc aggagaaatg gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa
     3601 aaaagcagac aggcgacgaa gtgaccactc cgtcgcttta caaagagagg aaaatcagag
     3661 gttgccggtg tagtgccagc gtaaataacg cagcaaacga agctgacgct taatgcggct
     3721 cggctgcgaa agcaggcggt agagccactc cagtcccagc gtttgccaga ttttcggtgc
     3781 gcgttttacg tgaccggtga aaacatcgta agtcccgcca acgcccatat acagcgcatc
     3841 tggatgcacc agacggcagt cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac
     3901 ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac agcgcctgac gctgctcggg
     3961 tttaaaataa ccatcctgac tgccaacgat attcacattc cattggttgc gcagtttagc
     4021 ttcagtttgc gccagcactt caggtttacc gcccacaaga aataccggcg tcccttcttt
     4081 gcctgcgcgc gccatcagct cttcccagag atcggcaccg gcaacgcggg aaacctgcgc
     4141 ctgcgggtac tttttacgta ctgaacgtac aatgctgatg ccatccgcat atttaaattc
     4201 ggcagcgtta attaactccc tgacctcggc gttatcttca atggtcagca ttttttcggc
     4261 attaatggca accagcgttc cctgcttaag ctgcccgtca gcaaacagat aatcgagggc
     4321 atgctgcata tcacgccaac caatcaactg taagccacgc agcgtatagg tcggtgccgt
     4381 ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga gcgatgattt tgtacgttta
     4441 tgaatgagtc cggcgctttc aaaaagccag tacaacagtt ttgcgatcat cagacatgcg
     4501 ccgaagacca cgataaaaaa gactacgcgt gagacaaacg aatccagccc ttcacgtgcc
     4561 agcacgatca tattgaagat cgccccaaag cagaaactgt gcaatatcgc agccttatag
     4621 cgattagtct cgcggttgcc cagctcatac agccagtcga accatttgat gatcagtcca
     4681 accacaatcg ccccgagtgg gatgaacaac gcgccgccca tcaccaccag tgagcctata
     4741 agcgtaggcg agatcgccag tccggagtgg ttattcagta cttcccaggt aaagtaattg
     4801 gctgagttca gcaccatact cgggcgcccc ggccacagcc aggaagggat aaagacatag
     4861 aaatcgcgga caattggagc caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc
     4921 aacgccagat tctcccacgg tgaaaaggtg tcgcgagtga gatagagaaa cgtatagaac
     4981 gcttcatcgc cgctcacatt cattccatag cgttttaacg ccagccagaa catgccaaca
     5041 atccccagca cgcccgccgc cgccagcatc cacaacgaaa tccagccgcg aataatgcca
     5101 ataaacagga agatagcgaa tgcgatgatg atattggcgc gagtgccgcc gacaatcata
     5161 taagtcagca agccaaaggc gaccgtgctg acgaggaaaa acagccacgc tttgctgtcc
     5221 tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac
     5281 gccacgccgg agacttcact ggaaaagatc tgactgtagg agttaagccg gaacagcaaa
     5341 aagccgttgt gcatgaagaa gatgccgacg cttaccagcg cgatacccat caggatcacc
     5401 cacgtaagat tggtctccac gcggttcatg gtaaacagcg gacggcgcgg tacatcagca
     5461 acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca
     5521 gaaagcaaca cctgcaacaa gatttctggc ggcgcgacac caacatcaaa gcgaaatacc
     5581 agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag
     5641 acattgaagt taaagcgcac acggcgaaac tcaaaccagg tcagcgtggc aataaacagc
     5701 gtgcagagca gccagaccac aaacaggcca ctgaattgca gcagactcat gcgacctccc
     5761 cggcggcaat cgccaacgcc cgctgccagc cttgtagata gttagggcta aagaaggcaa
     5821 tggtgttttt atccgccgac gccaactggc gctgcgcttc acgcacaata tcctcgttga
     5881 gatcgtcggt agtaaacagc actggcaaat gctgttccgt catatcctgc cagaacggat
     5941 tttccctgtt aagcacacaa ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc
     6001 cctgctggcg ggcaaaaata aagtaaccaa gatcgcactg acgaagtagc gccagatagg
     6061 cgtcaaattc cagtttttcg ctcaaaattt gcagattttc ttcgctgaat aactccagcc
     6121 ccgcctgacg aacttcctca atgtacgctt cgttattagg cggatatccc atcggcacca
     6181 ccacttttac cgtatcgcca aattgctgat gaacggcacg taaagcggcg acatgctcat
     6241 tgctgcggtc gccggagttc cccaccagaa tggtcatttt tccttcacgt tgccgatcgt
     6301 tcgccatcgt attgagagaa gggtccatcc gcgtcgggaa gtacagcagt tctccccgca
     6361 cctttgggtg tgttttggca aaaaagctca aatcgccgcg ggtggcaaat acacagccga
     6421 ctcgcttttg cgccaggcga cgtagtgggt aaaaaagctt atatctcaag ccactggaaa
     6481 gctcatacag gtctgccccc cagatatgcc agaaaaactg gctgggctta atgccaccac
     6541 tcagcagagc cagccacagt gtgggattga actgaccgtg gaaaaaaaat cgctgctgac
     6601 ggttagcttt tgctttcgcg atgaccgctt ccgccagcga ttttttccca gggaaaaatt
     6661 gcacagaaag cgccggacag ctatcactta agccgtcgtc cttgccaaca accataaact
     6721 cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt
     6781 tatggtgagg gatatccgat cccagtacgt gaatcagtac agtcatgccc gcctacgcca
     6841 gagtaaaaac acgccacaac aaagagaaaa atagacgata taagttgcca tatatgcctg
     6901 cgccgcgccc agtgcaccat gcgcagggat tagccagtgg gcaaatacca tcaataaagt
     6961 gaactggctg acttccgcca gaatataaaa ccgcagtgac gctttggcga tcaccagata
     7021 accaaagaca taagcgccca cttttaacac atcacccact aactgccagg caaagagatc
     7081 gcgcatagcg gtaaatttat tcgacaacag caaccagata gcaaaatcac gcagcaacca
     7141 gacggtaaaa ctcgctgccg ccactgccgg taagacgaat ttcagcgatt taaccacttc
     7201 ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa
     7261 cgatgccgta ataaattgca ggtaggcatc ggaaatactg ctcacccctt gccagatccc
     7321 cacttcatcc cagctatact gcgccgccag cagtttacgc atcatgatgt aagcaacagg
     7381 caaggtcacc gacgtaatca acgccatgag cgtaaatttg ctcaactgcc ctgccagacc
     7441 gttatcccag ctgggtttca gatagcttaa cgggatgaca ccacgtttaa tcaacatgat
     7501 ggcggcagga attaccacca gcgcgggaat cagcgccaga cccagcaacg ccccttcata
     7561 accgcccaaa cggtaactga cgtagtacgc gagaacgcca atcaagctgc cgacaatcag
     7621 cgataacgca ttacctgcgg catcgcgaaa gcctttcatc agcgccagta acaggttgcc
     7681 ccaggcgatc cccatttgca ccagcgccac taaacgcacc agcccctgat agtcggtatt
     7741 accaaacaat ccctggctga ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt
     7801 agagaaacca agtaccatcg ctgatgaagt gccgaccacg cggcgcagct gttgcggatt
     7861 atcatggtac tgggcaacgt atttggttac accgttaaag atgccagccc cggcaagcac
     7921 gccgagcacg gtaatcaact ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa
     7981 tgacaccgcc agcaacttac cgaccagtaa cccggcacca atcttgacca gtgtactggc
     8041 cgccgtccac aaggacgctt ttgccaacga catatcagga gaagtagttc aacaaagtcg
     8101 caattaccgt acgctgattg acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc
     8161 gctcgctctc tttggtggtg tagcgatctt caccgtggaa ctcaccaaag tgttcccccg
     8221 cagggcaacc gtgcagcgga atgtaatgaa acaccgccat gatttccgct tctttcagaa
     8281 agttaatcaa cgcgctccgg tcatcaatat cccgcagttt aatgtagaac atatgcgcgt
     8341 tctgcacgca gccatcggga atcgacggca gctcgatacg cccggctttc gccagaggcg
     8401 ctaacgtatc gtagtagttt tgccacagcg ccagacgttg ctggttgata cgatccgctg
     8461 cttccagttg cgcccacagg tacgcagctt gcagatcgga catcaaatag ctggagccaa
     8521 tatcgcgcca ggtatattta tcgacctgac cacggaagaa ctggctgcgg ttagtgccct
     8581 tttcacggat gatctcggct cgttcgatta acgctttatc gttaatcagc gtcgcgccgc
     8641 cttcaccacc cgccgtgtag tttttggttt catggaagct aaagcagcca atatgaccaa
     8701 tggttcccag tgcacgccct ttgtaagtgg acatcacgcc ctgagcggca tcttccacca
     8761 caaacaaatt atgctttttc gccaacgcca taatggtgtc catttcgcag gccacacccg
     8821 cgtaatggac cggcacgata acgcgcgttt tgtcggtgat cgctgcttca atcaacgttt
     8881 cgtcgatgtt catggtgtcc gggcgaacat ccacaaagac gattttcgcg ccacgcagca
     8941 caaaggcatt ggcggtggag acaaaggtgt agctcggcat gatcacttca tcgccaggct
     9001 ggatatcgag cagcagcgcc gccatctcca gcgaagcggt gcaggacggc gtcagtaaca
     9061 ctttggcgct gccaaaacgt tgctccagcc actgctggca gcgacgggta aaaccgccat
     9121 cgccacacag tttgccgcta cccattgccg actgcatata gtcgagttcg gttcccacca
     9181 ccggcggtgc gttaaatgga atcatgtgat cacctgtata accagtacgc ggtgctttct
     9241 acattcgcac cactttgtat gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc
     9301 acccgcaaag ttgttttacc gcgagcatac gcccagttta gcgccgtttg catcagctca
     9361 gcacctgcac cgcgtccagc cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt
     9421 aaagagacat agccgcgaat atcgccggac gccgcacgta gaatcagaca ttgatgatca
     9481 aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc
     9541 gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg cgctagctaa ctgacgtaat
     9601 gcgggaatat cggtctcttg tgccacgaca gcaccgctat cactgacatt gttcacgggt
     9661 agcgccaaat caacttcacc ttctaccagg gagaatccca gctgttgcag ggcatccagt
     9721 tcccccgtat ttgatgccgc aattttggcc tgcacccgtg accatggcgc taaggcgtct
     9781 gacgtcagga gcggtgcttc agacgtaatg cgcacgatgg cgctgttaac accaaagaag
     9841 gcgttttccc aggttagtgg ctcaatactg gcgcggacgg gcacgaagta actccagcag
     9901 atattggccg tagccagttt tcgctaatga actggcagca cgcttcacac cctcgtcatc
     9961 gagccagccg ttacgccagg cgatctcttc caggcaggca atcttaaagc cctggcgttt
    10021 ttctactgtc tgcacaaaag tgctggcctc aatcaggctg tcgtgagtgc cggtatccag
    10081 ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg cccgcctcga ggtacatctg
    10141 gttgatggag gtaatctcca gttcaccgcg ctccgacggc ttcacctgct ttgcgtactc
    10201 cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc cagtttgact tcggctgttt
    10261 tggcttttct tcaagcgaga tagcgcggaa attgtcgtca aattccacca cgccaaagcg
    10321 ttccgggtcc atcacctgat agccaaaaac cgtcgcccct tccgtgcgcg ccgcaacatg
    10381 acgcagcttc ggactgaagc cctgaccgaa gaagatgtta tcgcccagca ccagacaaga
    10441 aggttcacca ttaaggaagg tttcaccgat gataaaggcc tgcgccagac cgtccgggct
    10501 gggctgttcg gcatattcca gctggatacc gaactcacta ccatcgccca gcaggcgctg
    10561 gaaataacct ttatcttccg gcgtagtgat gatgagaatt tcgcggatac cggccagcat
    10621 cagcaccgac agcggatagt aaatcattgg cttatcgtaa atcggcaaca gttgcttcga
    10681 tacgccgcgc gtaatcggat gcaatcgggt accggaaccg cccgccagga taataccctt
    10741 catgtttgcc tccgctgaaa attagccttt cagacctaaa cgctcaccct gatagctgcc
    10801 gtcctgcacc tgcttccacc agctttcatt agccagatac cactgaaccg ttttacgcat
    10861 tccactttca aaggtttcct gcggcagcca gccaagttca cgggcaattt tcgaagcatc
    10921 aatggcataa cgcagatcat gccccggacg gtcagcgaca aaggtgatca ggtcacgata
    10981 gtacgccacg ccgtgcggct tgttcgaagc cagttcttcc agcagctcgc aaatggtttc
    11041 cacaacatcg agattcttac gctcgttgtg accaccaata ttataggttt caccgacttt
    11101 cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct tccacataca gccagtcacg
    11161 gatttgctgc ccgttgccat ataccggcag cggtttaccc gccagcgcgt tgaggatcat
    11221 cagcgggatc agtttttccg gaaagtggta aggaccgtag ttattcgagc agttggtgat
    11281 cagcgtcggc agaccgtagg tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt
    11341 tgacgcggaa taagggctgc tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt
    11401 cgagtgcagg tcaccatata cttcgtcggt ggagatatga tgaaaacgga acgctgattt
    11461 tttatcttcc gtcagcgtat tccagtaagc ccgcgccgct tcaagcaatg tataagtccc
    11521 gacaatgttg gtttcaataa acgctgccgg gccgtcaata gaacggtcaa catggctttc
    11581 ggctgccaga tgcatgacac agtctggctg atgctcagtg aatacgcgtg ccagttctgc
    11641 ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag
    11701 cgacatcagg tttccggcgt aggtcagctt atcgaccact accaccgcat cgctcgtttc
    11761 gttgatgata taacgcacca gcgccgagcc aataaacccg gcaccacctg ttatcagaat
    11821 ttttctcatc agcgccagac tcctttggca tcgacgacat actgctgatg gacattgtcg
    11881 ccattgataa ctttgaactg actatgatcg accagcatca ccagcacgtc tgccgttgcc
    11941 agcgcctcgt caagctgcgc cagagtacaa agcccggtca gttttttcgg caactggtgg
    12001 atgttaggct caacaaccag cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt
    12061 tccatcgccg ggctttcgcg caggtcatca atattcggtt taaacgccag accaaagcag
    12121 gcgattttca gttcactggc tcgtttatcg gtagcagcaa ggcagtcggc aaccgccgct
    12181 ttcacctgat cgataaccca gaacggtttg tgatcgttaa cttcgcgcgc ggtacggata
    12241 agccgcgcct gctgggggtt ctgtgccacg ataaaccacg gatcaacagc aatgcagtga
    12301 ccgcccacgc cagggccagg ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga
    12361 atcagttccc agacgttaat cccctgatcg gcacaaatca gcgacaattc attagcaaaa
    12421 gcgatattca catcgcggaa gctgttttcg gtgagcttac acatttccgc cgtccgcgag
    12481 ttagtgacga cacactcacc ttcgaggaaa attttgtaca attcgctggc gcgggccgaa
    12541 caaaccggcg tcataccacc aatcacgcga tcgtttttaa tcagctcgac cataacctgg
    12601 cccggtaata cacgttccgg gcagtaagca atgttgacgt ccgcctgctc gcccacctgc
    12661 tgtgggaaag tcagatccgg gcgcatctct gctaaccatt ctgccatctt ctcggttgac
    12721 cccaccggcg aggtggattc aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg
    12781 gagcgagcag ccgattcaac gtaggtcata tctggctcat gatcgccttt aaacggcgta
    12841 ggtacagcaa tcagccaggc atccgcttca actggcgtcg tgctcgctcg taaaaaaccg
    12901 ccttctacgg cagtttttac tacgctcgcc aaatcaggct cgacgatatg gatttcgcca
    12961 cgattgatgg tatcaaccgc gtgttggttg atatcgacac caatcacctg tttttgccgt
    13021 gaggcaaacg ctgctgctgt aggcagcccg atgtaaccca gtccgataac agaaatggtc
    13081 gcaaaactca tagtgatatc cgattatttt ttaacgcttc cagaatgcga gagcatgcct
    13141 gaccatcacc atacgggtta tgggcgcggc tcatagcttg atattcgttt tcgtctttta
    13201 aaagacgcgt cacttcctcg acaattcgct gcttatccgt gcctaccaga cgcaccgtac
    13261 ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat caccagcacc ggtttcccca
    13321 gcgaaggcgc ttcttcctga atgccgcctg agtcagtcaa aatcagccag gcgtggttca
    13381 tcagccagac aaacggtaaa tactcctggg gatcgatcag aatgacattt ttcacatgcc
    13441 ccagaatgcg attgaccggc tctctgacgt tcgggttgag atgcaccgga tagacaatct
    13501 ggatgtcctg gtgcgtggtg gcgatgtctg ccagcgcgtg gcagatttct tcaaagccac
    13561 gaccgaaact ctcacgccta tgaccggtca ccagaatcat ctttttatcg ggggcgataa
    13621 acgggtaatt tgccgccagt tctgaacgca gcgtgtcgct gctcatcacc tggtcacgca
    13681 cccataacag tgcatcaatg actgtattac cggtaatgaa gattcggcta tccgcaacgt
    13741 tttcacgcag caagttttgc cgggaagttt cggttggaga gaagtgatac atcgccagat
    13801 gcccggtcaa tgtacggtta gcctcttccg gccacggcga atagagagcg cccgtgcgca
    13861 gaccagcctc aacgtgacca acaggaatac gctgataaaa cgccgccagg ctggttgcca
    13921 gcgtcgtcgt cgtatcgccg tgaaccagca cgacgtctgg tttgaactcg gcaagaatag
    13981 gttttagccc ttccagaatc cgacaggtta tctctgtcag gccctgtcct ggctgcatta
    14041 tgttgagatc gtagtcaggt acaatggaaa agagtttcag tacctgatca agcatctccc
    14101 gatgctgcgc agtgacgcaa actttagcct caaaaaaagg atcttttgcc aacgcatgca
    14161 ccaacggagc catcttgatg gcttccgggc gcgtaccaaa tacagtcagt actttcacat
    14221 cgattctctt cgaataagcg gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc
    14281 gagcaacggc gggttaatgc gacaccagcc ccgatcagcc ccccgacaat gccccacata
    14341 atcatcagga aggcacgacg tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa
    14401 tagcgatagg tctgaaaacg cggatccagg gttggaccaa cattcagggt gtttaacatg
    14461 gctcgattct gatcatagtc gagatcaaag gccggaccga cggcctgtaa attttccagt
    14521 cgagcctgga gcattggacg cccaagcagg aacatttctg aatcaggtaa ttcctcggca
    14581 ggcacatctg tcgcactgcg cgaaatatta tgctgctcag caattttcag cgcctgctca
    14641 atactgttca tccggcggtc gtagatggct ttcgccacct cttcctgacg cttcacctga
    14701 gctttcatct ggatggtgcg tgccgcccat gcgcctttca gctcatcatt cagatggctg
    14761 gctgcacgct ggctggcaaa agcaacatac tgacgtaaca ggttattagc gtcaggcgcg
    14821 gtttcggcaa taagcttcac gctgtcattg accgcgcgag taaagtctcc ggggataaac
    14881 tggatgttgt taatcatttc atccagcaac gccgcatcgg ctttgctgtt gcccaccatc
    14941 cgctgtttgt aatagtcggt ttgcagccag aactctctgc gggtatccca cgaggccagt
    15001 tgcataacaa actctttata ggcttcgtcc atgaccgatg gttgatcggc agaagccatg
    15061 tttgaacgga catccaggtt acgcaaaaat tgctgctgcg agtaatatcc ccccagcata
    15121 ttcaccgttg gacgatcggt aatcgccgtc gagctccact cctgacgagc aaaaaaagta
    15181 tacgccagcg cgattaacgc aaacgccagc cccatgccaa taatccatag cttcccagcc
    15241 cacaaggtac gaaacaaccc acgaatatcc agttcatttt cagcgtcttc ggccggtttc
    15301 ccaggcattg gttgtgtcat cacatcctca tttatttggt taaattgggg ctgccaccac
    15361 gatttctacg cagtctgcgt tttacgcgct taataaagcg agcaactttc caggcacgct
    15421 taatgcaata tccatagagg aagaatgcta gcaaaaagag caccagcatg acccactccg
    15481 ggacaaaatg agaatattct gccagcacgc caatggaagc gagcagtgct gcggcaaggg
    15541 taatcagcac aaacgcctga cgggaagtaa acccggcacg catgatcaaa tggtgaatat
    15601 gctgacggtc aggagagaat gggctcatgc ctttacgcag gcgacggtac ataatcgcca
    15661 ccatatccat tagcggaatg gcgattatcc acaaagcggt aaccgggctg atgggatggg
    15721 ttttgccctg ggtcgtttcg agcaggatcc agataacggt aaaaccaatc agcgtactgc
    15781 ccgcatcacc cataaagact ttgtagcggc gacccaggat accaaggtta agcatgatgt
    15841 atggcaggat ggcggcgatc atcgcaaagc accagattgc gaggctggtt tgcccgtcga
    15901 accacaaaat cataccgatt gctgcaaacg agacgcagga caacccgccc agcaagccat
    15961 caatgccatc aaccatgttg aacgcattaa tggccgccca gacggcaaat agcgtcagga
    16021 agtaaccaaa cggtccgagc accatctccc aggagccaaa gatataaccc aggctactga
    16081 gataaagttt gccgaacacc atcataacaa tgccaacagc ggcctgtatg gtggcacgga
    16141 tttttacgct gatatcaaaa cgatcatcca gcgcgccaat gaaaacaagc acaccggcac
    16201 aagcgagata gagagatgca tgcggaatat agtaatcgac aattccgaac gtgaagcaaa
    16261 tccctgcgta aaccgaaatc cccccaacga gaggtatcaa tccctggtga cgtttgcgga
    16321 agtttggttt atccactaaa ccgacttttt ttgccacctt acgggcaaaa aacagaaaca
    16381 gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc
    16441 atgctctcag agaaaattat tagcagaagt ataaccacga agacctttat tcagaaggga
    16501 aagtccgttc cgaacccacc agccctgtgc aaaaatcaac catttgatat gcaaattgca
    16561 taacatgcca gcaaattcca ggaattttcc taacatcgcc agcgcgacat taagattaca
    16621 gatataaaag caaaacgcca cgtaaacacg tggcgttttt ggcataagac aaatttatga
    16681 gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc atggccagtt tattaatgag
    16741 gaattccatt gcatcgattt cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt
    16801 ctgcagttct tcctgagtcg tgagcagctc ttctttacgg gtaccggaac ggttgtagtc
    16861 gatagccggg aagacgcgtt tttcagcgat cttacgagag aggtgcagtt ccatgttgcc
    16921 tgtaccttta aactcttcgt aaataacttc gtccatttta gaaccggtat cgataagcgc
    16981 cgtcgcgata atggtcaggc tgccgccctc ttccacgtta cgcgccgcac caaagaagcg
    17041 tttcggacga tgaagggcat tggcgtccac accaccggtc aacactttac ctgacgccgg
    17101 aacaacagtg ttgtaagcgc gcgccagacg agtgatggag tcgagcagaa tgataacgtc
    17161 tttcttgtgc tcaaccaggc gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg
    17221 gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca ccttttacca gacgctgcat
    17281 ctcggttact tcttccggac gttcgtcgat cagcagaacc atcagcacac aatccgggtg
    17341 gttgtaagca atgctctgag caatgttctg cagcagcata gttttaccgg ctttcggcgg
    17401 tgccacaatc agaccacgct gaccacgacc gataggtgat gccagatcca gtacgcgagc
    17461 agttaaatct tcagtagaac cgttaccacg ttccatacgc agacgagagt ttgcgtgcag
    17521 cggggttaag ttctcaaaga ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac
    17581 ttcgttaact ttcagcagcg caaaatagcg ttcaccttct ttcggcgggc gaatcttacc
    17641 agagatggta tcaccagtgc ggaggttgaa acggcggatt tggctagggg aaacgtagat
    17701 gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg aggaaaccaa atccatcctg
    17761 caatatctcc agtacgccat caccaaagat atcttcgcca ctctttgcgt gctgcttcag
    17821 gatggcaaaa ataatgtcct gcttacgcat gcgagccagg ttttccagcc ccatattttc
    17881 gccgagagtg atcagctcag aaaccggcgt attctttaat tcggtaagat tcatagtggt
    17941 gtgagttctt aaacttgggg taaatctcga acttaatgtt gtgaatggta tggcaggatc
    18001 atccatgcct gtttacggtc atcgactcat gtcttttcgc tgcctggtca caggagagta
    18061 cgcagaactg aaacgacaag acggattgag tgacgagcca gaaatctgtt cacttcgcat
    18121 ttaagataaa acgggaagcg ttggaaatta caagattcaa acttaataag gtatgtttaa
    18181 tacgaagtca acactaagtt agcatgactc acgccgggcg tccagttttt agcgacgggg
    18241 cacccgaaca tgaaattccc ttacgccagg ttagcgtcga ggaactcttt caactgacct
    18301 ttagacagtg cgcccacttt ggttgccgcc acttcaccgt ttttgaacag cagcagagtc
    18361 gggataccac ggatgccata tttcggcgca gtgccagggt tttgatcgat gttcagtttt
    18421 gcaacggtca gtttgccctg atattcgtca gcgatttcat ccagaatcgg ggcgatcatt
    18481 ttgcacggac cgcaccactc tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt
    18541 acatccgtgt caaaactgtc gtcagtcagg tgaataattt tatcgctcat atataactcc
    18601 acaggaataa gcctggcgtg ttggtttcgt tgttggtgta acattaacca actaaaggtt
    18661 gactttattt caccggatac gctttcgtaa agcaatagta agctgatatt ctaccacact
    18721 atgagcaaaa cacatttaac agaacagaag ttttccgact tcgccctgca tccgaaggtt
    18781 gtagaagccc ttgaaaaaaa agggtttcat aactgtacgc ccattcaggc actggccctt
    18841 ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg
    18901 atggcgtttc ttacgtcaac gtttcattat cttctctctc atcctgcgat tgccgatcgc
    18961 aaggtgaatc aaccgcgtgc cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc
    19021 cacgccgacg cggaaccgct ggcggaagct accggcctga agctgggcct ggcttacggt
    19081 ggtgatggct atgacaaaca gctgaaagtg ctggaaagcg gcgttgacat tctgattggc
    19141 accacgggtc gtttaattga ctacgccaag cagaaccaca ttaacctcgg tgccattcag
    19201 gtagtggtac tggacgaagc cgatcgcatg tacgatctag gctttattaa agatatccgc
    19261 tggctgttcc gccgtatgcc gcctgcaaat cagcgcctca acatgctgtt ctccgccacg
    19321 ctttcgtacc gggtacgtga actggcgttc gagcagatga acaatgccga atatattgaa
    19381 gtggaaccgg aacagaaaac gggccaccgt attaaagaag agcttttcta cccttctaac
    19441 gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag agtggccaga cagagcgatt
    19501 attttcgcca ataccaaaca ccgttgtgaa gagatctggg gccacctggc ggcagatggt
    19561 catcgtgtcg gtttgttgac aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat
    19621 gaatttaccc gtggcgatct ggatattctg gttgccaccg acgttgccgc gcgtggtttg
    19681 catattccgg cagtgacgca cgtctttaac tacgatttac ccgatgactg tgaagattac
    19741 gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg
    19801 tgtgaagagt atgcattgaa tttgcctgct attgagacct atattggtca ctcaattccg
    19861 gtaagcaaat acaatccgga cgcattgatg accgatctgc caaaaccgct gcgcctcacg
    19921 cgcccgcgca caggcaatgg tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca
    19981 ggttaaataa aatgtcatac tcaaaatcat tcaaactgta tcaagatggc aagagaatga
    20041 atcccgatga gcttactgta gtaagtgatt cgggtgaaag aatgcagcca acacagagac
    20101 agattgaagg atgaagagta tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg
    20161 ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct ggaagcatcc agacgctgac
    20221 gcgaattaaa cgcaaagtgc gtctggctgc tggcctgaat agcgaaaatg acctctctaa
    20281 tgaagcaatg gagcgcggtt ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat
    20341 ccctccctcg caaattcgcg ttgtcgctac ggcgacatta cgacttgccg tcaatgcggg
    20401 tgattttatt gccaaagcgc aggaaatcct cggttgtccg gtacaggtga tcagcggtga
    20461 agaggaagcg cgtctgattt atcagggcgt tgctcacacc actggtggtg ccgatcagcg
    20521 cctggtggtg gatataggcg gtgccagtac tgaactggta accggcacgg gtgcacaaac
    20581 cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg ctggaacgct attttgccga
    20641 tcgtaatctg gggcaagaaa attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg
    20701 tccggttgcc gatgaattac ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac
    20761 cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg gatgaacgca ttaccctgga
    20821 aaagttgcag caattgaaac agcgagccat tcattgcggt cggctggagg aactggagat
    20881 tgacgggctg acgctggaac gtgcattagt gttcccgagt ggtctggcga tcctgatcgc
    20941 catttttacc gaactgaata ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg
    21001 cctggtctac ggcatgttgc atctggccgt cgagcaggat attcgcagcc gtacactgcg
    21061 taatattcaa cgccgcttta tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc
    21121 ggctaacttc ttcgatcagg tggaaaacga atggcatctt gaagcaataa gccgcgattt
    21181 gctcatcagc gcctgtcagc ttcatgaaat cggcctgagc gttgacttca aacaagctcc
    21241 gcaacacgct gcttatctgg tgcgtaacct ggatcttccc ggttttaccc ccgcacagaa
    21301 gaaactgctg gcgacgctac tgctcaacca gactaacccg gtcgatctct catcgctgca
    21361 tcagcaaaat gccgtaccac cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc
    21421 tatcattttt gccagccgtc gccgtgacga tctcgtgcca gagatgacat tacaggctaa
    21481 ccatgaactg ttgaccttga cgcttccgca aggttggcta acccaacatc cgctgggtaa
    21541 agagattatt gctcaggaaa accagtggca gagctatgtc cactggccgc tggaagtgca
    21601 ttaatgagta agtgccggat gcgatgctga cgcatcttct ccggccttga ttatttccct
    21661 cgttttgccg ccatcatcgc tttcagattc gccagatggc tttgcccttt ctgcatccgt
    21721 tcttcggcac tgaccacttt gcgctcctgt tcccaaatca gatcatcctg cggcagctcc
    21781 agcaaaaagc ggctcggctc cgggcgcacc agttcgccgt actgacggcg ttctttacac
    21841 agcgtaaagg tcaattcctt ctgggcgcgg gtaatgccga cataggccag ccgccgctcc
    21901 tcatcgatat tatcttcatc gatactgctc tggtgcggca aaaacccttc ttccataccg
    21961 accatgtaga cataaggaaa ctccagccct tttgacgcgt ggagagtcat cagttgcacc
    22021 tgatccagct cttcttcact ctcaccacgc tccatcatgt cgcgtaaagt aaagcgcgtc
    22081 accacctggg tgagcgtcat cggctcatcc agttcgctgc cttccagcat ctccgtcatc
    22141 cagctaaaca gttggttgac gttcttcatg cgcatttcgg cagctttcgt gctgggcgat
    22201 gtttcgtaca gccaggattc ataatccatg ccgtggatca gatcgcgcac cgcggcaatc
    22261 ggctcccgct ccgccagacg ctggatttct gccaaccagt gagtgaagcg ggtcaatgct
    22321 tcataaccac gtccgctaag cgtctgactc aggcccatat caaagctggc ggtaaacatg
    22381 cttttattgc gcgtcatcgc ccactcaccc agctttttca gcgtcgccgg gccaatctct
    22441 cgcttcggcg tgttaacgat acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg
    22501 cgcagataag ccagcaagtc cttgatttca ggacgagaga aaaacgacgt accaccagaa
    22561 attttgtacg ggatgcggtt ttgcatcagg aatttttcaa acacccgcga ctgatagtta
    22621 ccgcgataaa gaatagcgta atctttgtac tgcgttttat tgacgaagtg atgcgcaatc
    22681 agttcaccag tgacgcgctc ggcttcatgt tcttcgttat tcgcgcttaa aacttttagc
    22741 tctgtgccgt aacccagttc ggagaacaga cgcttttcaa agacgtgcgg gttattggcg
    22801 atcaggatgt tcgccgcttt caaaatacgt ccggaagagc ggtagttctg ctcaagctta
    22861 atcaccttca gcgccggaaa atcctggctc agcagcacca ggttttgcgg acgtgcgccg
    22921 cgccaggagt agatcgactg gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc
    22981 accagcagtt tcaccagttc atactggctg gtgttggtgt cctgatattc gtccaccagc
    23041 aggtagcgaa ttttgttctg ccagcgctcg cggacttctt cattgcgttg cagaagtagc
    23101 gtcggcaata aaatcagatc atcgaagtcg agcacattac aggctttcag gtgtgcatca
    23161 tacagcccat aacaatgggc aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc
    23221 tgggacggtg ttttgagatc gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg
    23281 agaactttgt catcttcaat cagcccttcg gtcaactctt tgagcaacgc cagctgatcg
    23341 gtatcgtcaa acagcgaaaa gttcgccttc atccccagcg ccgcatactc acgcttgatg
    23401 atatccagcc ccagcgtgtg gaaggttgag atcatcagcc catgcgcctc tttgcgaccc
    23461 agcgtctgtc cgacacgctc tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc
    23521 gcgatatgtc gcgcctgata accgcacccg cggatcagat gggcgatttt attggtgata
    23581 acacgagttt taccggaacc cgcacccgcc agcaccaggc aggggccggt aacgaattcg
    23641 acagcttgtt gttggccggg gtttagacgc ataggaaaat cgctcaatga aagtcgggag
    23701 gggaaaataa cagcgtggta gtatagccag cctcatcctt accactcaag gcacgatcat
    23761 ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct
    23821 tctcgtgcag attaagaacg gtgccgattt cggcaagctg gcgaagaaac actccatttg
    23881 cccatcaggc aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc
    23941 gttcgataaa gttgtgttct cttgtccggt actggagcca accggcccgc tgcacaccca
    24001 gttcggttac cacatcatta aggtgctgta ccgtaactaa tagcaaggcc ttctccggga
    24061 gaaggccttg aactctgaat cgctattaac ccgcaacagc aatacgtttc atatccgtca
    24121 tatagccgcg cagtttctta cctacttgct caatcgcatg gctgcgaatc gcttcattta
    24181 catcacgcag ctgcgcgtta tctaccgcac cttccggaat agctttaccc aaatcgcccg
    24241 gttgcagttc tgccataaac ggtttcagca gcggcacaca agcgtaagag aacagatagt
    24301 taccgtactc ggcagtatca gagataacca cgttcatttc atacagacgc ttacggacga
    24361 tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat tcttcgatga
    24421 tgccggaatc aaccatggtt tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca
    24481 tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac tgcggcgcgg
    24541 tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta tcatcgttgg
    24601 cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc atatgtttct
    24661 ggaacagcgg tgccatgatc tctttcagct gttcagacaa cgcgtaagca cgcagtttcg
    24721 ccgggttaga gagacggtcc atcatcagtg tgataccgcc ctgcttcagc gcttcggtga
    24781 tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg ccctcttcca
    24841 ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg atggtttgct
    24901 cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg cccgcacggt
    24961 gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct ttcggatcgt
    25021 tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac tcttcacgca
    25081 cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatgtcttta cggatctgct
    25141 cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg tctttcatca
    25201 gtggctgtac ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg ttaaccacca
    25261 gatccgcctg tgggatcagt tcttcgtaag tacccacttt aaaaccattt tcggtcgctt
    25321 tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag gagatatcga
    25381 gaccagaatc acgcatgttc agaccctggt tcagaccttg tgcgccacag ccgacgatga
    25441 ctactttttt accctgaagg tagctcgcgc catcggcgaa ttcatcgcgc cccataaagc
    25501 gacatttgcc cagctgtgcc agctgctgac gcagattcag tgtattgaag tagttagcca
    25561 tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt
    25621 cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca cattgcaatt
    25681 tctgcaacgt caacattgag ggctgcgcca ttggatttac gcgatctgaa aaccttcctg
    25741 catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt tagcccgtcc
    25801 acgctctcac ggcagattca gcgcctggaa gaagatctcg gccagccgct gtttgtgcgc
    25861 gataaccgca cggtgacgct gactgaagcg ggcgaagaac tgcgcgtttt cgcccagcaa
    25921 acgctgttgc agtatcagca gctgcgccac accatcgacc agcaagggcc gttgctctat
    25981 ggcgaattac atatcttctg ctcggtgacc gccgcttaca gccatctgcc gccgattctg
    26041 gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcactaccgg tgatgcggca
    26101 gatgcaatgg aaaaagtcgt cacaggtgaa gctgatctgg cgattgccgg gaagccagag
    26161 actctgcccg gcgcggtggc gttttcgatg ttggagaatc tggcagtggt gttgattgca
    26221 cccgcattgc cctgcccggt gcgtaatcag gtttctgtcg aaaagccgga ctggtcaacg
    26281 gtaccgttta tcatggccaa tcaggggccg gtgcgccgcc gcattgagtt gtggttccga
    26341 cgcaataaaa tcagtaaccc gatgatttac gccacggttg gcgggcatga agcgatggta
    26401 tcgatggtgg cgctcggctg tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc
    26461 cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt
    26521 gagctgggcg tgtgtgcgca gaaaaagcgg ctacatgagc cgctaattga ggcattctgg
    26581 aagattttgc cgaaccatta acccgccaaa aagaacctga acgccgggtt attggtttcg
    26641 tcgtggcaat cgtagcccag ctcattcagc cgggtttcga aatccggttc atggtcgcca
    26701 agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa
    26761 gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc
    26821 gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc gccgaccata
    26881 tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct gtagccgccg
    26941 tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg
    27001 acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt gaccgatcgc
    27061 ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat ggtcaccgcc
    27121 aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg caggccgtgg
    27181 aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg
    27241 gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc caccgcacgc
    27301 acatcttcga ataaatcctt catcgccgca cagatcgcat cgctatcgac ggtgatgatg
    27361 tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt taccgcgaca
    27421 ccttcggcga acagtcccac gcgtggcaga tcaaccggat gacccgcatc cagcgctgct
    27481 ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgcgg catcagttgt
    27541 ttgatcagca ccgctacgcc cgccgccaga ccgccgccgc cgactggcac aaatacgcgg
    27601 tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc ggcaatcacc
    27661 atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag ttcgatcgct
    27721 ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt
    27781 accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc cttcacgcct
    27841 aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc agtgatcacg
    27901 ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta
    27961 aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac
    28021 gacagttttt ccattttttg tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc
    28081 agcactgctc ttaaatattc ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc
    28141 attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca
    28201 ggctggcgta ggcgcacagg gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg
    28261 ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca tcgcttacct
    28321 gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct tcaatcagac
    28381 caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga ccagaggtgc
    28441 cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc attgatttca
    28501 ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg ccttcatagc
    28561 ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct accgcgtcgt
    28621 cctggctttc gtacactttc gccgggccgg tgaatttgag gatgctgtca tcgacgcccg
    28681 cggttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc aggccgccgt
    28741 ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg tcatcgagag
    28801 aatcccaacg gcaatcctgc gagaatgcct gtgtagtacg aatgcccgca gggcctgcgc
    28861 ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgt tccagagttt
    28921 gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc gcgcgatcca
    28981 gttcaccgag aataccgata acaccaccag cacggtgaac atcttccata tggtattttt
    29041 gggtgctcgg cgcaactttg cataactgcg ggactttacg cgaaagtttg tcgatatcac
    29101 tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt accgtgttgg
    29161 tagagccgcc catcgcgata tccagcgtca tggcattttc aaacgccgct ttgctagcaa
    29221 tattgcgcgg cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa
    29281 tgcgtttacc ggcattgagg aacaactgct tacggtccgc gtgggttgcc agcagcgagc
    29341 cgttgcccgg ctgcgacagg cccagcgctt cggtcaggca gttcattgag ttagcggtaa
    29401 acatcccgga gcaggagccg caggtcggac acgcggaacg ttcaacctga tcgctctggg
    29461 agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg agtttgatta
    29521 tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca aagatcaccg
    29581 gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg cagttagaga
    29641 tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa tcagcgatca
    29701 gttcgcgaga tggaagtgaa taaagcatcc ccccgtggcc catggcaatc ccatcatcca
    29761 ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca
    29821 gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa
    29881 ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac
    29941 gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta ggcatacttt
    30001 atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt
    30061 tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt gaagaggccg
    30121 aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc aatctgaata
    30181 ccgtctacgc tgcgcactgg cgtgatttct gccgccgtac cggacataaa gacttcatcc
    30241 gccaagtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc cagctctttc
    30301 gccagtttga tgatggcatc acgggtaata cccggcagcg cggaggaggt gaacggtggg
    30361 gtgaacagca caccatcttt cacttcaaac aggttttcgc ctgcgccttc agagatataa
    30421 ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc gctacccacc
    30481 agcagggaag agagatagtt accaccggct tttgccgcag ttgggatggt gtttggtgct
    30541 gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc tgcgcccaga
    30601 tacgctcccc acgggaaagc ggcgataatc acatcggttg agtatcccgc tggcgggttt
    30661 actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct ggtgagattg
    30721 tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg tgaaaccggg
    30781 aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg gcggaataca
    30841 accggtcctt tgtgtgagtc gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc
    30901 agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc cccattgaac
    30961 caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc aggcgcggat
    31021 ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt
    31081 taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt cgatatttat
    31141 attttgtgca tcgctggcgg cagccatatt cattgagcag acgtggaaac cacgatgacg
    31201 caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata cattgacctg
    31261 atgttgcatc atgataattt ctccaacatt tctgaattac tggcacctgg cggcaccagc
    31321 ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc atcactgttc
    31381 agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg gccagggatg
    31441 ccgaaggcgc tggctaacat gaggaaatcg gggttatcgg taagggtggt ttcgctgtat
    31501 cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt
    31561 aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg cacattcatc
    31621 atgaaagagc cgtcaccgga gatacagaca acggtatcgt tcggtcgcgc gacttgtgcg
    31681 ccaactgccg ccggtaaacc aaaacccatg gtacctaagc cgctggaggt gatgaaattt
    31741 tccgggcgag tgtgggcgat gtgctgcgca gcccacatct ggtgctgccc cacatctgtg
    31801 gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa cggagcgtag
    31861 atagcgtcac cgggatggtc gtaacgccag gaatgttcat cacgcagctg cgcgcagtgt
    31921 tgctgccagt cattgatatt taacggctgc tgtaatgctg gtaacagagc atttaaatca
    31981 ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc gatatccata
    32041 tggataacac tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa
    32101 cgtgcaccca cggcgatcag caggtcacac tcctgcaccg cgaagtttgc cgctttggtt
    32161 ccgtgcatcc ccagcatgcc cagatagtac ggataatctg cttctactgc gcccagccct
    32221 ttcagcgtac aggtggcagg catttttgtg gcagcgagaa attcacgcaa agccggaact
    32281 gcctgcgcca tccccacgcc accgccaacg tacagcatcg gtttttgcgc ttttgccagc
    32341 atctggcgcg cttgctcgac ttcggcatgt gggaaagtca cttcgttttc aacggtggtg
    32401 aaccacggtt ccagatcgcc gctggctaac tggatatctt ttgggatatc gaccagaacc
    32461 ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccatgatgcg cggcaactct
    32521 tccagagact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc caggacatcc
    32581 acttcctgaa atgcgtcggt gccgataaac ggtgcggaca cctgaccagt gatggcaacg
    32641 acagggatgg aatctaacag tgcgtccgca agcccggtta tcaggttggt tgcgcccgga
    32701 ccagacgtgg cgatacatac gccagtttta ccggtagcac gggcataacc gatagccgcc
    32761 attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc atacaatgca
    32821 tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac accctgtgcc
    32881 cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg
    32941 agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt ttaaggtcaa
    33001 aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc
    33061 tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcacg
    33121 accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag
    33181 gaatgcctaa agagttacca tagattttgc gaatagcaca agatattttt gggcctattt
    33241 ttatgatggc tttaataatt tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt
    33301 gaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc ctttgtccgc
    33361 tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg cacaaataaa
    33421 caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat cttatgcaca
    33481 taatggtgac tcaaggaggg tttatgtcac tgtcaattgt tcatacccgc gcagccctgg
    33541 aagtaaatgc gcccccaatc actgttgagg tacatatcag taaaggtcta cccggcttaa
    33601 cgatggtggg cttaacagaa acaacggtga aagaagctcg cgatcgcgtg cgcagcgcca
    33661 ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt gctccagccg
    33721 atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg ctggcggcct
    33781 cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa ctagcgctta
    33841 caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct attaagtcgg
    33901 gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag
    33961 gatgcctgat agccgatcat ctgcaggctg tctgtgcgtt tctggaaggt aagaacactc
    34021 tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat ctcagtgatg
    34081 ttgtcggtca ggagcaagga aaacgaggac tggaaattac cgccgctggt gggcacaacc
    34141 ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt attaatggcc
    34201 tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta agtctggtaa
    34261 atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct catcacagtg
    34321 cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa atttcgttgg
    34381 cgcataacgg cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt acactggatg
    34441 ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa
    34501 cctatccagc ccgtttccag cttgttgcgg cgatgaatcc cagccctacc ggacattatc
    34561 agggaaacca taaccgctgc acgccagaac agacattacg ttatctcact ggagatccca
    34621 ttaccacccc ccggcatttt gagtaaaacg gtagtgccgg gagaaagcag cgccaccgtt
    34681 aaacaacgcg taatggccgc cagagagcgc caatttaagc ggcagaataa actgaatgcc
    34741 tggctggata gtccggaaat acgccaattc tgcaaacttg agagcgaaga tgcgatgtgg
    34801 ttggaagaaa cgctgatcca tctggggtta tcgattcgtg cctggcagcg gttactgaaa
    34861 gttgcacgaa ccattgctga tattgatcag tctgacatta tcacacgtca gcatttgcag
    34921 gaggcagtta gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa actactgaca
    34981 taaaaaaagg gcatttcgcc ctttttatta atcgtcagaa tcggtgtagt cttcagcacc
    35041 ttcaacctgc ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct taatacgcgt
    35101 catatacttg gaccacacgc gttctgcttc tgtcactggc tcacgttcgc cacggcatac
    35161 tgctacaaag agtttctctt cctcggtaac cggctcgcgt ttgccaagat ccaactcatt
    35221 gaaggcataa ccatgacgct caagcagttg tgcctctttg atggtgaaat caccatgacg
    35281 agagaatcca cgtggataat gtttattgtc gaaatatcga ttagtcgtcg taaagctttc
    35341 cgccatcctg cacgctccta attctttgac cgagctagtt atggcgcgga gtattagtta
    35401 cgcttgacag agtgtaaaac aaaacattta aatcataacg acaaataatt ttgcggagag
    35461 cactgtggat acggaattgt taaaaacttt cctggaagtt agccgaacgc gtcactttgg
    35521 tcgagcggct gaatcgctct atctgaccca gtcagcagtg agctttcgaa tcagacaact
    35581 ggaaaatcaa ctgggtgtga accttttcac ccgccacaga aacaatatcc gtttaaccgc
    35641 tgccggtgaa aaactactgc cttatgcaga aacgctcatg agcacctggc aggccgcccg
    35701 caaggaggtg gcgcatacct cacgacataa cgagttttct atcggtgcca gcgcctcgtt
    35761 gtgggaatgt atgcttaatc agtggttggg acgcttgtat caaaatcagg atgcccatac
    35821 aggcttacag ttcgaagcgc gaattgccca acggcagtct ctggtaaaac agctgcatga
    35881 acgccagctt gatcttctta ttaccactga agcgcccaaa atggacgaat ttagtagtca
    35941 gttgctgggg tatttcactt tagcgcttta taccagtgcc ccttctaaac taaagggaga
    36001 tcttaactat ctgcgactgg agtgggggcc agattttcaa cagcatgagg caggtttgat
    36061 cggtgctgac gaagttccca ttctgacaac cagttctgct gaactggcac agcaacagat
    36121 tgcgatgctt aatggttgca cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct
    36181 gcataccgtt gtcgatagca caacactttc acggccgctt tatgccatat ggctgcaaaa
    36241 tagcgataaa aatgcgttga ttcgcgatct tttgaaaatt aacgtgctgg atgaagtgta
    36301 ttaatctgaa tggctggcaa ggatgccggt agaaggattt acttcggaga ggattatttc
    36361 agataaaaaa aatccttagc tttcgctaag gatgatttct ggcaggggcg gagagactcg
    36421 aactcccaac acccggtttt ggagaccggt gctctaccaa ttgaactacg cccctaatta
    36481 gggtggcgga acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta
    36541 accgactgaa ctaccgctcc accgaattct tttacaacca ccggtttta
//
LOCUS       DAJTNP010000031        35471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTNP010000031 DAJTNP010000000
VERSION     DAJTNP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            37..285
                     /locus_tag="OO160_001712"
                     /pseudo
     CDS             37..285
                     /locus_tag="OO160_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(332..1090)
                     /gene="deoR"
                     /locus_tag="OO160_001713"
     CDS             complement(332..1090)
                     /gene="deoR"
                     /locus_tag="OO160_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5523870.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1148..1744)
                     /gene="ybjG"
                     /locus_tag="OO160_001714"
     CDS             complement(1148..1744)
                     /gene="ybjG"
                     /locus_tag="OO160_001714"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5523871.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2029..3250
                     /gene="mdfA"
                     /locus_tag="OO160_001715"
                     /pseudo
     CDS             2029..3250
                     /gene="mdfA"
                     /locus_tag="OO160_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(3291..3575)
                     /locus_tag="OO160_001716"
     CDS             complement(3291..3575)
                     /locus_tag="OO160_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523872.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3661..4476)
                     /gene="ybjI"
                     /locus_tag="OO160_001717"
     CDS             complement(3661..4476)
                     /gene="ybjI"
                     /locus_tag="OO160_001717"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5523873.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4476..5684)
                     /locus_tag="OO160_001718"
     CDS             complement(4476..5684)
                     /locus_tag="OO160_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5523874.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5768..6304
                     /gene="rcdA"
                     /locus_tag="OO160_001719"
     CDS             5768..6304
                     /gene="rcdA"
                     /locus_tag="OO160_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5523875.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6479..8164)
                     /locus_tag="OO160_001720"
     CDS             complement(6479..8164)
                     /locus_tag="OO160_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5523876.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8434..8811
                     /locus_tag="OO160_001721"
     CDS             8434..8811
                     /locus_tag="OO160_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5523877.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8841..9098)
                     /gene="grxA"
                     /locus_tag="OO160_001722"
     CDS             complement(8841..9098)
                     /gene="grxA"
                     /locus_tag="OO160_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5523878.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9258..9545
                     /locus_tag="OO160_001723"
     CDS             9258..9545
                     /locus_tag="OO160_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5523879.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9529..10251
                     /gene="nfsA"
                     /locus_tag="OO160_001724"
     CDS             9529..10251
                     /gene="nfsA"
                     /locus_tag="OO160_001724"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5523880.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10312..11214
                     /gene="rimK"
                     /locus_tag="OO160_001725"
     CDS             10312..11214
                     /gene="rimK"
                     /locus_tag="OO160_001725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5523881.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11302..11778
                     /locus_tag="OO160_001726"
     CDS             11302..11778
                     /locus_tag="OO160_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5523882.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12130..13242
                     /gene="potF"
                     /locus_tag="OO160_001727"
     CDS             12130..13242
                     /gene="potF"
                     /locus_tag="OO160_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5523883.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13337..14470
                     /gene="potG"
                     /locus_tag="OO160_001728"
     CDS             13337..14470
                     /gene="potG"
                     /locus_tag="OO160_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5523884.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14480..15433
                     /gene="potH"
                     /locus_tag="OO160_001729"
     CDS             14480..15433
                     /gene="potH"
                     /locus_tag="OO160_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5523885.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15430..16275
                     /gene="potI"
                     /locus_tag="OO160_001730"
     CDS             15430..16275
                     /gene="potI"
                     /locus_tag="OO160_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5523886.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16335..16823
                     /locus_tag="OO160_001731"
     CDS             16335..16823
                     /locus_tag="OO160_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5523887.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16864..17991
                     /gene="rlmC"
                     /locus_tag="OO160_001732"
     CDS             16864..17991
                     /gene="rlmC"
                     /locus_tag="OO160_001732"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5523888.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18166..18897)
                     /gene="artJ"
                     /locus_tag="OO160_001733"
     CDS             complement(18166..18897)
                     /gene="artJ"
                     /locus_tag="OO160_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5523889.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19189..19857)
                     /gene="artM"
                     /locus_tag="OO160_001734"
     CDS             complement(19189..19857)
                     /gene="artM"
                     /locus_tag="OO160_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5523890.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19857..20573)
                     /gene="artQ"
                     /locus_tag="OO160_001735"
     CDS             complement(19857..20573)
                     /gene="artQ"
                     /locus_tag="OO160_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5523891.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20580..21311)
                     /gene="artJ"
                     /locus_tag="OO160_001736"
     CDS             complement(20580..21311)
                     /gene="artJ"
                     /locus_tag="OO160_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5523892.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21329..22057)
                     /gene="artP"
                     /locus_tag="OO160_001737"
     CDS             complement(21329..22057)
                     /gene="artP"
                     /locus_tag="OO160_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5523893.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22275..22790)
                     /locus_tag="OO160_001738"
     CDS             complement(22275..22790)
                     /locus_tag="OO160_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5523894.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23439..25208
                     /locus_tag="OO160_001739"
     CDS             23439..25208
                     /locus_tag="OO160_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5523895.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25419..25742
                     /locus_tag="OO160_001740"
     CDS             25419..25742
                     /locus_tag="OO160_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5523896.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25739..26569
                     /gene="amiD"
                     /locus_tag="OO160_001741"
     CDS             25739..26569
                     /gene="amiD"
                     /locus_tag="OO160_001741"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5523897.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26566..27579)
                     /locus_tag="OO160_001742"
     CDS             complement(26566..27579)
                     /locus_tag="OO160_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5523898.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27678..29108)
                     /locus_tag="OO160_001743"
     CDS             complement(27678..29108)
                     /locus_tag="OO160_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5523899.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29119..30120)
                     /gene="ltaE"
                     /locus_tag="OO160_001744"
     CDS             complement(29119..30120)
                     /gene="ltaE"
                     /locus_tag="OO160_001744"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5523900.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30157..31875)
                     /gene="poxB"
                     /locus_tag="OO160_001745"
     CDS             complement(30157..31875)
                     /gene="poxB"
                     /locus_tag="OO160_001745"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5523901.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32008..32976)
                     /gene="hcr"
                     /locus_tag="OO160_001746"
     CDS             complement(32008..32976)
                     /gene="hcr"
                     /locus_tag="OO160_001746"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5523902.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32988..34640)
                     /gene="hcp"
                     /locus_tag="OO160_001747"
                     /gene_synonym="priS"
     CDS             complement(32988..34640)
                     /gene="hcp"
                     /locus_tag="OO160_001747"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5523903.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34784..>35471)
                     /gene="lysO"
                     /locus_tag="OO160_001748"
     CDS             complement(34784..>35471)
                     /gene="lysO"
                     /locus_tag="OO160_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5523904.1"
                     /translation="ILLCNIAALMWLERGLPWRNHHQQEKLPSRIAMALESLKLCGVV
                     VIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQIVLNRRGMIVAVV
                     VVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFGPVIGSAAFFNDLA
                     RELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMVPAAIVHGFILSLL
                     VPILIAFFSA"
BASE COUNT         8799 a         8791 c         9116 g         8765 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaccg tgaccatacc gcgtgggcag
       61 ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc agcttaccgc accgctgaaa
      121 aaaggtcagg ttgtcgggac cattgatttc cagcttaacg gtaaatccat tgagcagcgt
      181 ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtaggat gtgggatttc
      241 gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc tgataaccgg
      301 atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata cggtgcgtct
      361 gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata tcaaagcgtt
      421 tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg acaaccagca
      481 catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca agattaaaac
      541 aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata tccgggcaaa
      601 aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg gcgtgaaatt
      661 caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga aaggtattta
      721 gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata atccacggcg
      781 tggtggtgcc acagtcaaaa aagagggtct gatcgggttc taccagtgtc gcagccagtt
      841 ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac aggtaatggc
      901 tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc gcactgtggt
      961 tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cagggcggcg gcgtctttaa
     1021 gatgtaactt atcgctgcgt tttaattctt gcagcagctg cccgatacgc tcttcgcgac
     1081 gtgtttccat aatccctctg aatagttatt gaagcgagcc gctcaatact acacttttta
     1141 gcagagatca gtcacgcacc cagcctttgc ggatcggtaa tgcaaaacag acgcgatacc
     1201 acgattgcag acgttgatag agtttatgcc ccatcgcttg ccagataatc tgggcactaa
     1261 ggcagccaat catacctgcc agcaatccac cgagcatatc cagcggccag tggacgccaa
     1321 gataaacgcg cgaccaggca atgacgacgg ccagcaccat taaaagtgag ccggaccaca
     1381 ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat caccgtaccg tgatcgctcg
     1441 ggaatgagtc atccgccgca tgatgcagga agttatagcc gatattttcg acaaagggtc
     1501 ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa caggctgacc gccagcgcga
     1561 tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc ccacaaccaa agtaccgcgg
     1621 ccagcaacgg caccacggta atcaaatctt tagcaataaa aatcgccaac gagatcatcc
     1681 acggagccga gtctggcgtc gcgttaataa gagagaatag agagagattc aaattttcca
     1741 gcataacttc ccgacgcaaa gtgattaaaa gggagccaat acaggcaagt cgttgagaat
     1801 aaagtgcagg ttaaactggg taaagcggca tcgtcttatt tccctcaagc ggcctgttta
     1861 cggtgggtga ttgtaacggg cataggttaa ataaaactta aagaaagcgt agctatactc
     1921 gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac aaaattcgct ttctcgttag
     1981 ctgcgctttt attaaactct gcgcgattat tattggcgaa gaaattgcat gcaaaataaa
     2041 ttagcttccg gtgccaggct tggacgtcag gcgttacttt tccctctctg tctggtgctt
     2101 tacgaatttt caacctatat cggcaacgat atgatccaac ccggtatgtt ggccgtggtg
     2161 gaacaatatc aggcgggcat tgattgggtt cctacttcga tgaccgcgta tctggcgggc
     2221 gggatgtttt tacaatggct cctggggccg ctgtcggatc gtattggtcg ccgtccggtg
     2281 atgctggcgg gtgtggttta tcgtcacctg tctggcaata ttactggcgc aaaacattga
     2341 acaattcacc ctgttgcgct tcttgcaggg cataagcttc tgtttcattg gcgctgtggg
     2401 atacgccgca attcaggaat ccttcgaaga ggcggtttgt atcaagatca ccgcgctgat
     2461 ggcgaacgtg gcgctgattg ctccgctact tggtccgctg gtgggcgcgg cgtggatcca
     2521 tgtgctgcct tgggagggga tgtttgtttt gtttgccgca ttggcagcga tctccttttt
     2581 cggtctgcaa cgagctatgc ctgaaaccgc catgcgtata ggcgagaaac tgtcactgaa
     2641 agaactcggt cgtgactata agctggtgct gaagaacggc cgctttgtgg cgggggcgct
     2701 gggattcgtt agcctgccat tgctggcgtg gatcgcccag tcgccgatta tcatcattac
     2761 cggggagcag ttgagcagct atgaatatgg cttgctgcaa gtgcctattt tcggggcgtt
     2821 aattgcgggt aacttgctgt tagcgcgtct gacctcgcgc cgcaccgtac gttcgctgat
     2881 tattatgggc ggctggccga ttatgattgg tctgttggtc gctgctgcgg caacggttat
     2941 ctcatcgcac gcgtatttat ggatgaccgc cgggttaagt atttatgctt tcggtattgg
     3001 tctggcgaat gcgggactgg tgcgattaac cctgtttgcc agcgatatga gtaaaggtac
     3061 ggtttctgcg gcgatgggaa tgctgcaaat gctgatcttt accgtcggta ttgaaatcag
     3121 caaacatgcc tggctgaacg ggggcaacgg actgtttaat ctcttcaacc ttgtcaacgg
     3181 aattttgtgg ctgtcgctga tggttatctt tttaaaagat aaacagatgg gaaattctca
     3241 cgaagggtaa aaaaatgcct gattgatttg tgcgatcagg cattctcgaa ttaatggtga
     3301 tggtcgtcaa tctggtgttc gataaccatc ccttcaccta cgctggcaag atggcgaaca
     3361 taaggatgcg ggcggtaagc cggagctggc gcaggagcca catagacggt ttgcggcaca
     3421 gtcgcgacgc tgaccgcttg tggaacgctg actgaatcag gaaccacaac cactttgtat
     3481 ccactcggca catcgacggt gacactttgc gccatcgcca caccagtaaa gcccattaaa
     3541 agcgcgccca acagtagaca atttttcata atcatttcgc ctgaaataat gcagcgggtt
     3601 aatttccgcg aattatgcag ccttcattca ggcattgtgt tgccgggcgt gctgccgttt
     3661 ttattggtca aatggcgctt cgtgtttaag aactttatcg atcacatcca gtacgccttc
     3721 acggttattg tagcctgccc ggtattttgc cgctgcgacg actgcgctgc cggcattttc
     3781 cattgcaaaa ctaaagcctg cctgacgcag catctcaata tcgttaccgc catcgccaaa
     3841 gaccaccact tcgctgtcgt ctattcccca taatttctgc agttggcgaa ggccattggc
     3901 tttatgtacg ccggggataa tcagatcgat gctgccgttg ccggtgtgga ctggcaccat
     3961 aatatcgccg atggcctcat gtaatgcttt ttgtacttgt ggaatcagtt catcggaaag
     4021 attcaggcca aacttaaaga aaatatcttc taagttgtca aagttatcga cgtattccag
     4081 acggtgataa tacatttccg ccaccgtttt catggcatcg tcatattttt tgagtgtata
     4141 ggcgctattt tttccgcagg caataatttc cacttccggg cgcgtcagca aatgttccac
     4201 gacagtagca aacgcatcct tcgacagctc gccattaaaa acatctttgc cttcactcat
     4261 tacccagccg ccgttttcag ccacaaaggc aatttcattc gcaatttcag ggaagaaaga
     4321 gatcaactga taatattgat tcccgctggc gaccacaaag cgaatgcctt gtgctttcat
     4381 ttgctgatac tgagccataa accgctcacg gttataggtt ttttgatcgc ttaagaaagt
     4441 accatccatg tctaccgcaa ttaatttaat gctcatcaac tattctccat cgccgtctgc
     4501 gttttggtat cgggtttggc gacggctttc gcgacaatag ccgcgagaat aaccagcgcc
     4561 agtacaacca gcattgcact acgtaatcca taatgttcgc cgagatagcc cagcagcggc
     4621 ggcccgacga ggaaagccag ataaccggtc gttgctacca cactgacgcg ggttggtgca
     4681 tcggggccgg tatcgctggc ggcagaaatg gtcagcggga agcccagcga ggcacccagt
     4741 ccccaaagta caacagacac tcctgcgacc caggcgctat cgacaaaaat aatcagccca
     4801 atacccaacg cccccattag tgcactggcc cgaaccacgg caacgcgact gtaactgtcg
     4861 atgaaccaac cgccggtgaa gcgtccaacg gtcatcccca gggtaaaacc ggcataaatc
     4921 agtgagccgg aagtggggct aaaaccgtga ccatcaacca ttaataaggg taaccagtcg
     4981 ttggcagaac cttcggcaaa ggccatcgcc agcactacaa caccaatcaa cagcaactgg
     5041 atatcgcgat aaaaaggaat gcctttttcg ccatgctggg tgccatcggc agcatttttg
     5101 cccgtaccgt cagggattgc ctgaatggcg atataaatag gcgcgatacc taccagcgcc
     5161 gccaataaaa tgtgcaccgt tgccggaacg ccaaaggccg tcagtgccat cccgacacca
     5221 gcgcctgcca gcgtgcccag gctataaaaa ccgtgcatca tcggcaaaac cgttttattc
     5281 atttctcgct caacggcggc accttcaacg tttatcgcca cttccgcaga accaaaactt
     5341 gccccaaaga cgccgagacc aacggcaaag agcaggggcg atgtcagcca gagtgccaga
     5401 cttaatatca tcatcccgat caatgcgcag gacatcgtga ccaggatgac attacgtgtc
     5461 ccaaagcgtt tcactaacca cgccgagcag agaataccgc tcatcgaacc gatcgacaga
     5521 ccaaagagaa caccgcccat ttcagcgatc gagacagaga gaatatcgcg gatagcaggc
     5581 gtacgggttg cccaggacgc cattaacagg cctggcaaaa agaagaacat aaacagcgcc
     5641 caggttcggc gtttcaatgc attacgtgaa gaatttacgg tcatagatca cgtcaaaata
     5701 agaagaggga agacaacatt agcaaggttg tgtacatttg tacacaattg cagacagagg
     5761 aaatgacatg cgtcgcgcta acgatccgca acggcgagaa aaaattatcc aggccacgct
     5821 ggaggcggtg aaactttacg gaatacatgc tgttacgcac cgcaaaattg ctacccttgc
     5881 cggggtaccg ttgggatcga tgacctacta tttttcagga attgatgagt tgttgctgga
     5941 ggcgttcagc agttttactg agattatgtc ccggcaatat caggcatttt ttagcgatgt
     6001 tagtgatgct caaggcgcat gccaggctat caccgatatg atctacagct cacaggttgc
     6061 aacgccggat aatatggagc tgatgtacca gctctacgcg ctggctagcc gaaaaccgct
     6121 attaaaaacg gtaatgcaaa actggatgca gcgcagtcag caaacgctcg aacaatggtt
     6181 tgaacccgga accgcccgcg cgcttgatgc gtttattgag gggatgacgc tgcattttgt
     6241 caccgaccgt aagccgctat cgcgcgagga gattttgagg atggttgaga gggttgcagg
     6301 gtagtagata aattttagat aacaaaaaac ccatcaacct tgaaccgaaa tggcggggtt
     6361 gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta agactgatgc
     6421 cctgcggaaa agttctgcgg ttgtgcaaaa aaatttcatt ttcagggcaa cttcagtttt
     6481 atcctaatcc tggccatacc atgacgatga ttgtccctgc cagcgtcagc aggacgttgg
     6541 cgattgcgta ggtgcccgca tagcccagcg cagggatgtt actgcgagct gtatcactga
     6601 tgatctccat tgccggcgcg caggtgcgtg cgcccatcat tgcgccgaac aacagtgcgc
     6661 ggttcattcg caatacataa gcaccgaaca agaaacagat aaccacgggc acaagactga
     6721 caattaatcc ggcaatcaac atctgaccgc caatcgcgcc caggccgtta ttaataccgc
     6781 taccggcgct cagaccaacg cctgccataa acaccatcaa gccgaactct ttcaccatgc
     6841 ttaatgcacc ttgcggaatg taaccgaagg tcgggtggtt agcacgcata aagcccagca
     6901 taattccggc gaataacaac ccggcagcgt tccccatgcc gaaactgaat gtgctgaact
     6961 ggaaggtgat catcccgatc atcagcccaa taacaaagaa ggcgcaaaat gccagcaggt
     7021 cagtgacctg gctgtgaatc gagataaagc cgatgcgatc ggcgatggtt tttacgcgac
     7081 gggcatcacc gctgacttgt aaaacgtcac ctttgttaag cacgacgttg tcatctatcg
     7141 gcatctcaat ctggctacga atgacgcggt taaggaagca accgtgatcg gtcaacttca
     7201 gttgtgcgag acgtttacct acagcgttat ggtttttaac gaccacttct tcagtgacga
     7261 tacgcatgtc gagaaggtca cgatcgaaaa cttctttacc gttacggaag ctgggatcga
     7321 gtcgggcatg ggcgtcggga tagcctacca acgctatttc atcgcccatt tgtagcacgg
     7381 catcaccgtc tggatttgcc agaatcccgt tacgtcgaat acgttcaatg tagcagccgg
     7441 tttgtcgata aatacccagt tcacgcagat ttttgccgtc ggtccaggcc accagctccg
     7501 ggccgacgcg ataggcgcgg atcaccggta aataaacctt acggttggca tcagtgtcca
     7561 ggccacgttc gcgggcgatt tgctgggcgc tggtctgtaa gtcctgatgc tgcaatttcg
     7621 gcaagtaacg cgcaccaaca atcaaactca ccagaccgat taaataggtt aaggcatacc
     7681 cgaggctcag attatccagt gccagtgaga gctgcctgct ttccatgccg gaatgatgca
     7741 gtgtatcgcc agcaccgacc agaaccggtg tcgacgtcat agagcctgct aacataccgg
     7801 ccgtcaggcc aatatcccag ccaaacagct tacctaaccc taaggcgatc accagcgcac
     7861 tgccaaccat caccagtgct aacattaggt aatttttccc atcgcgaaaa aaaatggaaa
     7921 aaaagttcgg tccggcttcg accccgacgc agaaaataaa cagcataaag ccaagattaa
     7981 gcgcatcggt gttaatgctg aaatgttgtt ggcctaataa cagcgatacg actaaaacgc
     8041 caatggaatt acccagttgg atcgaaccaa gtcgtaactt tccgagacat agcccaagcg
     8101 cgaggaccac aaataataac agaatgtaat tcccatttaa caattcggcg acgtttatat
     8161 tcacggaggc taacttcttg tttactagta agctgttgaa agaaatgcta atttacgata
     8221 atgtgtttta ccagaattca gggcgcagat tcattcagcg cacctaaacg atagtaaagt
     8281 aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga attgcgttgg
     8341 cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac tggcgaaaaa
     8401 tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg ttgggcgggg
     8461 gcaatctgct gttttgtcct cttcattgtg gtgtgccttt ttctggcgac gcacatgaaa
     8521 ggcgcttttc gggctgccgg gcatcctgaa atcggcttgc tgtttttcat tcttcctgga
     8581 gcagttgcca gtttcttttc acagcgtaga gaagtcctga aacctctatt tggcgcaatg
     8641 ctggcggcac cctgttcaat gctcattatg cggctgtttt tttcaccgac gcgctcattc
     8701 tggcaagagc tggcatggtt actaagcgcg gtgttctggt gtgcgctggg ggcactgtgt
     8761 ttcttattta tcagtagttt gtttaaacca cagcacagaa aaaatcagta aagccctcaa
     8821 cgcgagggct tgtcagacga tcaggcgtcc agattttctt tcacccatgc agcaaaatcg
     8881 gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc tacgggttta
     8941 cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc tacatactga
     9001 tactgaaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc acgcacacag
     9061 taagggcaac ccgaacgacc aaaaataacg gtttgcatta tttctctcct catagattta
     9121 tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct gttgcattaa
     9181 ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact gaaggctcac
     9241 tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaggcgtg ttgatactgg
     9301 aatttatcgg aatgatgcta ctggcggtgg cgctgctgtc ggtaagcgac tccctgtcgc
     9361 tgcctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc ggtgttttgc
     9421 tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt gccccacagc
     9481 tgatgaactg tccaccgcaa tattcacgtt cagaaagaga aaaagataat gacgccaacc
     9541 attgaactta tttgtggcca tcgctccatt cgccatttca ctgatgaacc catttccgaa
     9601 gcgcagtgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag ttttttgcag
     9661 tgtagtagca ttattcgcat taccgacaaa gcattacgtg aagaactggt gacgctgacc
     9721 ggcgggcaaa aacacgtagc gcaagcggcg gagttctggg tgttctgtgc cgactttaac
     9781 cgccatttac agatctgtcc ggatgctcag ctcggcctgg cggaacaact gttgctcggt
     9841 gtcgttgata cggcaatgat ggcgcagaat gcattaaccg cagcggaatc gctgggattg
     9901 ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgaa actgctgaaa
     9961 ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc ggataatccg
    10021 gatctcaagc cgcgtttacc ggcctccatt ttggtgcatg aaaacagcta tcaaccgctg
    10081 gataaagacg cactggcgca gtatgacgag caactggcgg aatattacct cacccgtggc
    10141 agcaataatc gctgggatac ctggagcgat catatccgcc gaacaatcat taaagaaagc
    10201 cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta aaaccgccac
    10261 gtcgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag ggtgaaaatt
    10321 gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga agccgctata
    10381 cagcgcggtc acctggttga aattcttgat ccgctttctt gctacatgaa cataaatcct
    10441 gcggcgtctt ctatttacta caaaggccgc aagttacccc attttgacgc agtgatcccg
    10501 cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt cgagatgctg
    10561 gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa attgcgttcc
    10621 atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc gcattcgccg
    10681 gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt caagttggtt
    10741 gaaggcacgc agggaattgg cgtcgtgctg gcggagactc gtcaggcggc ggaaagcgtg
    10801 attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat caaagaggcg
    10861 caagggtgcg atatccgctg tctggttgtt ggtgatgaag tggtcgctgc gattgaacgg
    10921 cgggcgaaag agggctattt tcgttccaat ttgcatcgtg gcggcgcggc aagcgtcgcc
    10981 agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat ggcgctggac
    11041 gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga agtgaatgcg
    11101 tcgccggggc tggaaggaat agaaaaaacc accggtatcg atatcgcggg taaaatgatc
    11161 cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg ttagtcgcaa
    11221 tcacattact gatcatggtt ttgcctgcgc tttttgcgta agctgtgccg gtctttttat
    11281 cgaaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga tacgctgcgt
    11341 caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca ggctctgcat
    11401 ctgccccata tgcagaattt cgacggtgtc tttgatgcca aaatcgatct gatcgataac
    11461 acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc gctggcggcg
    11521 gatttatctg ccatcaatgc cagttcgctg accgtgaaag catttcttga tatgcaggat
    11581 gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg cgtaacctat
    11641 gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt cattcttgaa
    11701 gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaacaa cgttaccgaa
    11761 aactattttc tccactgata attcctttcg agcacgcagt cgctgccgca gtgactgcgc
    11821 gctgcaaaat tatctgctgt ttttaacctt ttcttaaaga ttatttcact tctcttgtgt
    11881 cgatttggct ttatcacata gagcaaatac gcataaaaat ttgttaaata ccgtttttta
    11941 atccgagcta tagtctcaaa ccctggctaa agttattctt gcgatgcttc tatatagcga
    12001 gcagtgctgg ccgggagaga gttctctttt cttacaccgc gccgataaaa aaatatgcac
    12061 gtttattgca tatctttcag tgtgacaact tttgttcgtt tgttaacgaa ctttcagaag
    12121 gaaagagatg tgaccgcctt aaataaaaaa tggctatcgg gtctggttgc gggtgctctg
    12181 atggccgtct ctgtcggcac gctcgcggct gaacaaaaaa cactccacat ttataactgg
    12241 tctgattata tcgcccctga cacggtggcc aattttgaaa aagaaaccgg tattaaagtc
    12301 gtctacgatg ttttcgactc taacgaagta ctggaaggca aattaatggc gggtagcacc
    12361 ggctttgact tggtggttcc atctgccagc tttctagaac gccagttgac tgcgggagtt
    12421 ttccagccgc tggacaaaag caaattgccg gagtggaaga atctcgaccc ggagctgctg
    12481 aagctggtcg ccaaacacga tcccgacaat aaatttgcta tgccctatat gtgggcgacg
    12541 accgggattg gctataacgt tgataaagtt aaagctgtgc tgggcgaaaa cgcgcccgtc
    12601 gatagctggg acttgatcct caaacctgaa aatctggaaa aactgaaaag ctgcggtgtc
    12661 tctttcctgg atgcgccaga agaagttttt gctaccgtgt tgaattatct cggcaaagat
    12721 cccaacagca ctaaagcgga tgattacacc ggaccggcaa ccgatctgct gttaaagctg
    12781 cgcccgaaca ttcgttattt ccattcatct caatacatta acgacctggc aaacggcgat
    12841 atttgcgtcg ctatcggctg ggcaggtgat gtctggcagg cgtcaaaccg cgcgaaggaa
    12901 gcgaagaatg gagtgaatgt ctcgttctcg attccaaaag aaggggcgat ggcgttcttt
    12961 gatgtattcg ccatgccagc ggatgccaaa aacaaagacg aagcctatca gttcctgaat
    13021 tacctgctgc gcccggatgt agtagcgcat atttccgacc atgtgttcta tgtcaacgcc
    13081 aataaagcag ccacgccgct ggtgagtgcg gaagtccgtg agaacccggg tatttatccg
    13141 cctgcggatg ttcgtgcgaa gctgttcact ctgaaagtgc aggacccgaa aatcgaccgt
    13201 gtgcgcaccc gcgcgtggac caaagtgaag agcggaaaat aatccgcagc cttagatgcc
    13261 gggcgggcgc accacacccg ccggcaattc gcaccatcat ggtgcgcttg cacacattca
    13321 atgccggaga gcagccgtga atgacgctat ccctcgcccg caggcgaaaa cccgtaaggc
    13381 gctgacgccg ctattagaaa tccgcaacct caccaaatcc tacgatggtc aacatgcggt
    13441 ggatgatgtc agcctgacca tctacaaagg tgaaatcttc gcgctgctgg gcgcatccgg
    13501 ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttc gaacaacctt ctgccggaca
    13561 gataatgctt gatggcgtcg atttgtcaca ggttccgcct tacctgcgcc ccatcaatat
    13621 gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg
    13681 cctgaaacag gacaaactac cgaaagcgga aatttccagt cgggtcaatg agatgctcgg
    13741 cctggtgcac atgcaggagt tcgccaaacg taaaccgcat cagctttctg gtggtcagcg
    13801 acagcgtgtg gccctggccc gaagccttgc caagcgcccg aaactattac tgctcgacga
    13861 gccgatgggc gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat
    13921 tctggagcgc gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac
    13981 catggcgggg cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgaaccgga
    14041 agagatctac gagcatccga ctacccgcta tagcgccgaa tttattggct cggtaaacgt
    14101 ctttgaaggc gtactcaaag agcgtcagga agatggcttg gtgcttgatt cgccggggct
    14161 ggtgcatcca ctgaaagtcg acgcggatgc ctcggtggtc gataacgtgc cggtacatgt
    14221 ggcgctgcgc ccggaaaaaa tcatgctttg cgaagagccg cccgccaatg gttgtaactt
    14281 cgcggtgggg gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg
    14341 tctgaaaagt gggcagatga ttagtgccca gctacaaaac gcccatcgtc atcgtaaagg
    14401 gttaccgacc tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct
    14461 gacggtttaa ggagcaaaga tgaatacact tgaacctgct gcccagtcga aaccgccggg
    14521 cggatttaag ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc
    14581 gttgccatat atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat
    14641 aagcctggcg gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga
    14701 cgggcaactg tccatcactc ttaatctcgg taattttctg caactgaccg acgatccgct
    14761 ctatttcgat gcttatctcc agtcgctaca ggtggctgcg atttcgacat tttgctgttt
    14821 actgatcggc tatccgctgg cgtgggcggt ggcgcacagt aagccttcga cccgtaatat
    14881 tttattacta ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcttg
    14941 gatgggaata ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat
    15001 cgatcaaccg ctgaccattc ttcataccaa tctggcagtt tatattggca ttgtttacgc
    15061 ctacgtgccg tttatggtgc tgccgattta taccgcgttg attcgtattg attattcgct
    15121 ggtggaagca gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt
    15181 gccgctgact aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg
    15241 cgagtttgtg atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct
    15301 atggcaagag tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat
    15361 gttgctgctg ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg
    15421 agaacacgga tgaataattt accggtagtt cgttcgccct ggcggattgt gattttgctg
    15481 ctgggcttta cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc
    15541 tcgaagctgg tgacggtgtg gggcggctgg tcaacgcgct ggtatggtga gttattgcgc
    15601 gatgacgcga tgatgagtgc ggttggttca agcctgacaa ttgctgcctg tgcggcaacg
    15661 gcggcggcga tcctcgggac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc
    15721 ggatcaaatg gctttgcctt tatgatcacc gcgccgctag taatgccaga tgtcatcacg
    15781 ggcttgtcgc tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac
    15841 cgcggtatgc ttaccatctg gctggcgcat gtcactttct gtacggctta tgtggcggtc
    15901 gttatttcgt cgcgtctgcg ggaactggat cgctcgatag aagaagccgc gatggatctc
    15961 ggtgcgacgc cgctgaaagt gtttttcgtc attacgctac cgatgatcat gcccgcgatc
    16021 atttctggct ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt
    16081 gtttccgggc cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg
    16141 gtgaatccgg aaatcaacgc cctggcaaca ttgatcctcg gcgcggtcgg aattgtcgga
    16201 tttatcgcct ggtatctgat ggcgcgcgca gaaaaacagc ggatacgcga catccaacgt
    16261 gcaagacgtg gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgtc
    16321 gcggcattgt tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc
    16381 atgctcgcct gaatgtgcct gcgctggtgc aggtggcggc gctcgccatt attatgatcc
    16441 gtggcctcga cgtgctgatg attttcaata cgctgggcgt gcgcggtatt ggcgagttca
    16501 ttcatcgcag cgtacaaacc tggagtttaa cgctggtctt tttaagcagt ctggtgctgg
    16561 ttttcattga gatctggtgt gcgttttcac tggtgaaagg gcgtcgctgg gcgcgctggc
    16621 tatatctgct gacacaaatc accgccgcaa gttacttgtg ggcggcttcg ctggggtacg
    16681 gttatccgga gctgttcagc attcccggtg aatcaaaacg tgaaatcttc catagcctga
    16741 tgctgcagaa gctgccggat atgctcatcc tgatgctgct gttcgttccc tcgaccagtc
    16801 ggcggttctt ccagttgcaa taatgtgtat aatcgtcgcc cctgatgatg tgaaggtcaa
    16861 tgtatgcagt gcgcacttta cgacgcgggt cgctgtcgtt cctgtcagtg gataacgcag
    16921 ccgattccag agcaactctc cgctaaaacc gccgatctta aaaatctgct cgccgatttt
    16981 ccggttgagg aatggtgcgc gccggtgtca ggcccggaac aagggtttcg taataaagcc
    17041 aaaatggtgg tgagtggtag cgttgaaaaa acactgctcg gtatgctgca tcgcgatggc
    17101 acgccggaag acctttgtga ctgcccgctt tatccagcct catttgcgcc cgtttttgcg
    17161 gcgctaaaac cgtttatcgc ccgagcgggg ttaacgccct acaacgtggc gcgtaagcgt
    17221 ggcgaactga aatacattct gctgactgaa agccagagtg atggcggcat gatgctgcgc
    17281 tttgtactgc gttctgatac caagctggcg caactgcgta aggcgctgcc gtggttacac
    17341 gaacaactac cgcagctgaa agttattacc gtcaatattc agccggtaca tatggcgatt
    17401 atggaagggg agacggagat ctacctgacc gaacaacagg cactggcgga gcgttttaat
    17461 gacgtaccgc tgtggatccg tccgcaaagt ttcttccaga ctaatccggc ggtcgccagc
    17521 cagttgtacg ccaccgcgcg cgactgggta cgacagctgc cggttaaaca tatgtgggat
    17581 ctgttctgcg gtgtgggggg ctttggttta cactgcgcga cgcctgacat gcagttaacc
    17641 gggatcgaaa ttgcgccaga ggccattgcc tgtgcaaagc agtcagccgc tgaactgggc
    17701 ttaactcgtt tacaatttca ggcgctggac tccactcagt ttgccactgc tcagggggag
    17761 gtgccggagc tggtgctggt taacccgccg cgccgcggca ttggtaaacc gctgtgtgat
    17821 tatctttcaa cgatggcacc gcgttttatt atctactcca gctgtaacgc ccaaaccatg
    17881 gcgaaagata tccgcgaact gccaggttac cgcatcgaac gggtacagct ttttgatatg
    17941 ttcccgcaca ccgcgcacta tgaagtgttg acgctgctgg tgaagcaata aaaaagccac
    18001 aggtgcggct tcagattgct gatcaggtgc gctttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaat catgctaaat caatacattg cagagttgat gtgggccgga
    18121 taagcgcagc gcatcaggca atcttacttt tgtcatcagt ctcaattact gcggaaacca
    18181 ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg cgttattcag
    18241 tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg tgccgaagta
    18301 ttgcggatcg gtcacttttt cagtcgcaac gcccagttgc ggattggttt tcagccattc
    18361 gtttaccacc gcggtgtcac caaatacccc atcaatacga ccatttttca gatcgataaa
    18421 ggcattctga taactgtcat aggagacagt tttcacttcc gggtgctgat cctgaatata
    18481 tttctggtgc gtcgtaccgt tttccatccc aatgcgtttg cctttcaggt cggcaaacgt
    18541 tttgtaggta tcttttttgg aaatcacgac ggctgagttt tcatagtagg gtgtggtaaa
    18601 cgaaacctgc ttgctacgct ccggggtgat atccataccg gagattacgg cgtcatattt
    18661 tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag tacattctgc
    18721 ctgcatttgt ttgcacaagg cttttgccag atcgatatca aagccgacaa tctcattatt
    18781 agcacctata gattcaaagg gtggataggt ggctgaaacg ccaaaattga ttttctctgc
    18841 ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact ttttcatgat
    18901 ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg gataacaatg
    18961 ccatcaagtg aatttatatg caataaacat gattaaataa tttaaatgaa ataaaaaaag
    19021 acggataact tagtgagttg tccgtcttca ttataagaat ttatgcacta tgtaggccgg
    19081 ataaggcgtc cacgccgcat ccggcacagg caccgtgctg atgtctgatg cgacgctggc
    19141 gcgtcttatc agacctacaa acccccccgg cgaatgtacg cagctacatt aattccgccg
    19201 ttcgaatgcc agtgctttgc gctcgatcag acgcatcatc agcgtcagca ggccgttgac
    19261 gaccaggtaa ataatccctg ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa
    19321 caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag aggtactttt
    19381 gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg catacggcag
    19441 caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact gccactgacc
    19501 ttctgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg cactgttcag
    19561 cgataacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca gtgccggata
    19621 ctcctgcaac gtcggaaact ggcccggccc gtaataaatc aggaagatct gcaccagcag
    19681 cggcgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca ccggcgtttt
    19741 cagcgtcagg atgatggtaa aaatcaatgc cagaatcagt gccacaatca gcgaggcaac
    19801 ggttagcgtc aggctggtgt gcagcccttt catcagctcg ggtaaatact caaacattag
    19861 ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg
    19921 agcagggtga tcaccaggta aatcgccgcc gccacaatgt accaggtaaa tggttcctgg
    19981 gtacgagtag caatgctttt ggtttgtaac attaaatcat tcacactaat caaactgacc
    20041 agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag cgcatgacgc
    20101 cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc cagcgcctga
    20161 ccagattccc actggcccac cggtacagct ttcaacgcgc cgcgcagcgt ttgcgaggca
    20221 taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacggact cacgtcgaag
    20281 ttctcaatgt ccatctgcac cgggatctgc acgaacccaa gattgatagt gaagccatcc
    20341 gaaagcgtca gcagtaactg cgaggagcca aaatagataa acagcactac cagaatttct
    20401 ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg acgccatttc
    20461 gctgactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg
    20521 gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt catcaggaat
    20581 tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt cactttttcc
    20641 agcgcagtgt tgagtttctg ctgcagttca gtgtttccct gacgtaccgc gatgccgagg
    20701 ccagtaccga aataatcttt atcggtcact ttatcgccca ctgccgccag ttttgggtta
    20761 tctttcagcc actcagtgac cactgcggtg tcaccaaata cgctgtcgat acgcccgttt
    20821 tgcagatcca gttttgcgtt ctggtagctg tcatacggaa cggtggtgat ttccgggtgc
    20881 ttatccataa tgaacttctg gtgtgtcgtc ccgttctgaa cgccgacttt tttgcctttc
    20941 agctgatcaa cactggtgta tttgccttgc tgacccacaa acagggcaga gttgtcatag
    21001 tacggggtag taaacagtac ctgcttttca cgctccggag tgatatccat gcccgccatc
    21061 actgcttcta cgcgacggaa tttcaggctt gggatcaggc tgtcaaacgc ctggttagag
    21121 aaagtacagg ttgcatcaat ctctttacac agtgcctgcg ccaggtcgac gtcaaaacca
    21181 acgatctggt tgtttgcatc aatcgattca aacggaggat agggggcttc ggtagcaaaa
    21241 cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga
    21301 acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta aatgcttcgg
    21361 tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgacca ttttccatat
    21421 acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg atcacctggg
    21481 taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta atttccgggt
    21541 ccagtgcggc ggtcggttca tcgaacagca gtacctgcgg ttccatcatc aacgcacggg
    21601 caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga tcgctataag
    21661 gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga tctttactca
    21721 accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga tgcggccaca
    21781 ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag
    21841 agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca
    21901 tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg gggccaagta
    21961 acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc
    22021 cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata ctggcagtct
    22081 gatagctatt gaggtcgaag atagtacctt tgacagaata attatgcaat atttatgctt
    22141 taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac gggggcggtg
    22201 gtcggtgagc ataaatgtcg gcattcctca cgaaatgccg gataatttac ggggtttatt
    22261 ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc cgtggcatga
    22321 acgctaccgc ctaagtagcg cacatcgtcg atgacccaac actggccttc ctgaatcatt
    22381 aacacttcat cctgccagcc ctggtcaccc tgtttgagat ccacccgcaa tggaatgtta
    22441 cgggcatcac gattagggat agtcgatgca ctggcaacgt gggcgctgtc tggcaaggtg
    22501 gttcgactgg agaacggatc gctgcttaac aattcacgat gactattatc gcggctggca
    22561 tcgctaagtt gggttgccag tttgtcactc aaataggggc gcagggcggt aatgtcattg
    22621 ctgcgatgtt gaatgcggta gtcgtagaat tgttgagcca cactgtcagg ccctccctgg
    22681 acacaggaac cggtgcgggg gccgttatct ttataagctg gcgtgacggt ggtacaggca
    22741 ctcagcagca gtgcacatgg aataagtaga gatagtgaac ggtagcgcat aatcatttcc
    22801 ttattagctt gctgtctgcg tcacactacg accaaacgct atcagattat ttaatacatt
    22861 gtttttaata caattctgaa acaatctgaa cacctgattg ggtttgagtc cattgctggc
    22921 gacccactca aggttgctcg tagctgactt acttaaatta cccaaacaat agacagtgta
    22981 aaccatttgt gaggttgagc gtgttatatg gctgaaatga tcatttaagg taaatatgat
    23041 agttctgaaa tggtggtata aatcctcctg tagaggttaa gagcatctgc aagaagccag
    23101 tttcacattt ataacgcgtg ataaatgaat gcggttattg tctaatcatt acaaaatttt
    23161 tcacatgcga taagagatcc tgcgattaag atgattgcca aaattacgtt gtatttttga
    23221 aaatgagcga aatttgatag tcccagcttt gactctcgat aatattattt atgctacaat
    23281 tgtgggtaaa gaatttaagc taatgaattt gagtgaagaa taaattaaat tgcttcagta
    23341 tttcgttaat taatataaat acaaaaggcg ttgtgctttt ataattataa agcgcgagga
    23401 gttcggtgga ttatcgtttt tcttgggtga tttttaatat gataaacaat atatctgatc
    23461 aagcatcatc atttccaggc acacaactaa atcaaagtga taatttcctg gattcactga
    23521 gggaattttt cgcgatatta aatcccagtc gtaagggtga gttgtctaca tgggatacca
    23581 tctacttgca tctgatatta gcaataaatg ctgacagtga tctcataaaa aatgatgtgt
    23641 tgctcgcgga aaatattcca tcagcgaatt accaatttaa tacatttttt agcaatactt
    23701 tcgagattga tgttaaaaaa tatctgggta aatcagaaga taacgaagtt gaaataaaag
    23761 cgggtaatga aagaatctct attggaatac gtaatgtatc gaatggaaaa cttgaaagac
    23821 aacagtttct tttcccactg gactatgaga ataaattaca agaacaatta gataagtatt
    23881 ttacgattga atcgcaccca ttattataca ggtataccat tggtagtaag attgcgaatg
    23941 tgatttttga aaagttatac agtcgtattg actttaacaa agaacaatat atatccttta
    24001 taaaggacgc atttattcat ttttatgatt atagcaggcg atatgctatt agtgagaata
    24061 tcgataaaga cgctgtaaca aacaatatcg cattgatgag cactttttat gacagtgata
    24121 atacgtcagg tgaggtttta aataatgatt ttacagaaga agagtcattt gaaactgcgt
    24181 tagatgtaga gcatgcaata gtgcttggat ttgctgatga taattttgaa acaaaacctg
    24241 ttcattatca ggatttatta acacgattta gtgcctttca agataccgtg tttaatctgt
    24301 ttcctgaaat gcactcttcg cattaccacg atatttgtag cgtaagtgtg gatatgacaa
    24361 agggaacgca atgcatgatt cacttgatgg tgaatgaaga agttttcatg tctctgcctg
    24421 ttccagttgc cacgatggta cgtgaagatg caagtaattt agtcaatctt aaaacactac
    24481 tcaatgatgg ttgtttcatt aaatatagtc attttaatga cgtggcatta ataaagcaaa
    24541 acataagtaa tctctattta tcacacactg ttgtgaatga aagcatattg aaaaaatgtt
    24601 gttttgaaaa cggcagttta ggtgatgtta aaataacaaa tagcaatgta ataaacagtg
    24661 cattcaaaaa tatttcgttt agatctgtta aaattaataa cgtaaatact cattctttaa
    24721 aatttattaa ctgcaatttt ttcaatgttg atatgataag agttaatctc agcaaatgtc
    24781 tttttcatga atgtagtatg catggtgtca agataaaacc ctggttgcct gtaaaatgga
    24841 caaaagaatt aataagtgat tatttgtatg gctgtttact atctctgtac tctatctgtg
    24901 caagggatat atataatatg aacgccggga ataacgtgaa ggttgctgcg gatgctttcc
    24961 ttgaaattat tttctctctt aaaaataaat attgtattaa actattaagc gctcaggatc
    25021 gggcgtttat atacgagttt gcaagaatga tcttcgctta tattaatgac aaaagcattg
    25081 agatcttatt actttcttgt tttgcggcgg ctgaccaaaa agcaatccag cggtatagac
    25141 ctcagtcaca agatggggaa gactttagga gtcatttaca atataagttg cctctttccg
    25201 ctcattaatg ttgttggtgc aacataataa gacaggtaag cggtacccta taatgaaacc
    25261 aatatcgggt tatgcaacgg ataatcgtaa agacttaagt gggtgttttt gacacaatga
    25321 gatgatttcc taaactgatc aaaaccttct tcccaacatg gcatatcagt ccgataatgc
    25381 tttagagatc gaaggcttaa caaacaagga gatcgatcat gcaattttct acaaccccaa
    25441 ctctggaagg cctgaccatc gttgaatatt gcggtgtggt gaccggcgaa gcgattttag
    25501 gtgccaatat tttccgtgat ttctttgccg gtatccgcga tatcgttggc ggacgttccg
    25561 gtgcgtatga aaaagaactg cgtaaagcac gggagatcgc ctttgaggaa ttaggctccc
    25621 aggcgcgggc gctgggggcc gatgccgtag ttggtattga tatcgactac gaaacggtcg
    25681 ggcaaaacgg cagtatgctg atggttagcg tcagcggtac ggcggtgaaa acgcgtcgat
    25741 gagaagagtc ttctggctgg tcgctgccgc tctgttactg gcagggtgtg caggcgaaaa
    25801 aggcattgtc gagaaagagg gatatcagct tgatacccga caccaggcgc aggcggcgta
    25861 tccgcgcatt aaagtgctgg tgatccacta caccgcagat gattttgata gctcgctggc
    25921 gacactgact gataagcagg tcagctcgca ttatctgatc cctgcggtgc caccgcgata
    25981 caacggtaaa ccgcgcatct ggcaactggt gccggaacaa gaactggcct ggcatgcggg
    26041 gattagcgcc tggcgcgggg caacgcgcct taacgacacc tctattggca ttgagctgga
    26101 aaaccgtggc tggcaaaaat catccggagt gaaatatttt gccccgtttg aacaggcaca
    26161 gattcaggcg cttattccgc tggcgaaaga tattattgcc cgttatcaca tcaagccgga
    26221 aaacgtagtg gcacatgcgg atatcgcacc gcagcgcaaa gacgatccgg ggccattatt
    26281 tccctggcag caactggcgc agcaggggat tggtgcctgg ccggatgcgc agcgggtgaa
    26341 cttttacctt gccgggcgcg cgccgcacac accggtagat accgcgtcat tgctggagct
    26401 tttggcgcgc tacggttatg acgtaaaacc tgatatgaca ccgcgcgagc agcggcgcgt
    26461 gattatggca ttccagatgc atttccgccc gacgttatat aacggcgaag cggatgcaga
    26521 aactcaggcg attgccgaag cattgctgga gaaatacggg caggattagc gcggcagttt
    26581 tccgtggtcg cgtagccagg cggcagtttt ctcgatgcct tcatccaggg tgatgaccgg
    26641 ctgataacct aactcttcct gcgcacgcgt aatatccagc gtaaagtcaa aattcaactt
    26701 ggaggcgccg tagtgggtca gcggcggctc ttttgctgac ttgcggccta aacgctccat
    26761 gctgcgggcg atcatatcca gcatcgggta agggacggaa cgaatacgac agtcaatatt
    26821 caactcgtcg atcagcttct gcacgatgct gcgcagtgtg cgatgctcgc cgttggtgat
    26881 gttgtacaca cgcccggaag gtagcttatc gcaggcttcc tggcttgcca gccacattgc
    26941 gtgcacggca ttttcatagt aggtcatatc gaccagcgca ctgccgccat gcggtaacag
    27001 aatactgccg tagtggtgca tcatatgcgc cagacgggga ataaagactt tatcgtgcgg
    27061 tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt tgtggattcg cctgcgaaag
    27121 catattgatc acttcttcgc tggctgcttt gctgcgggca aactcgttgg cgaagcggtg
    27181 agggcgaaaa tcttctttaa tattgcgatg gtggtgataa tcgaagtaca gggaggggga
    27241 agagatatga ataaagttac gcacacccca ggcgacagcc cattcaccca ggcggcgagt
    27301 ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc cagggtgagg taaagctgga
    27361 gcagtgccac agcgtatcaa tgcccgcgag catcacttta gcttgtgatg aaaccagctc
    27421 ggtcagatcc gtcggaacaa actctgcgcc cattttttcc agcaatttac ccattgcttc
    27481 gttgcgaccg ctcgcacgca cgctgatgcc tttctggcat aaaaactcta ccgcgtttcg
    27541 acctaagccg ctggtggcgc cggtaaccag taccttcata tcaatccact gttgttgaga
    27601 aaatgacgtg cgcattcttc cgtgatttcc cccatgatgc aatgggaaac atgaaagaat
    27661 aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca gtctggcgat ttgttttgcc
    27721 attccgcgaa aaataaacag atgcgcgggg atcatcaata accagtaaaa cagccccggc
    27781 ataccgtgcg gatgccagaa agcgcggaca tcgatagtac gatagtcacc tttatcttcc
    27841 agggtaaaac acagtcgtcc cagccccggc gctttcatgc caaataacaa cgtaagttgc
    27901 tttttcggtt caacgacaat cactttccag ctatccaccg catcgccggt ctgtaaatat
    27961 tcgcgctccg ggcggccttt cgccagatta tgaccgatcg cgcggtccat caacgcccgt
    28021 gtctgccaca aaatattgcc aaagaaataa cgctctttac cgccgatttg gttcaccacc
    28081 agccataaag ccgcaaggct ggcggacgtt ttaacggtaa accccgcctg tttggcgaaa
    28141 taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt cgtagcccca gtcgctggag
    28201 ttgaccagtt tttcttcctc tttcaacgtg ctacgtaccg cgtcatcgaa agcgatcagc
    28261 cgttgtggga tgagtgcacg tagcgcggta tcatctgcca gcagatcgtg tttcagcccc
    28321 tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa tcacattgag aaaccacacc
    28381 gaaatccagc gggtggggag ggggatgggg atcaaccagc ggcgcttacc gctcaccgcc
    28441 ataaaatgtt caaactgttg ctgataactg agcacctctg gtccggcggc ttcgaagatg
    28501 cggtgttcgt tggctggatg gtctaacagc gccaccagat agtgcagcaa gttttccagc
    28561 gcgatgggcg tggtgcgtga acgtacccag cgtggcggcg ttaacactgg caggttgtag
    28621 accatatcgc gcatgacttc gaacgccgct gaacctgcgc caacgataat tccggcccga
    28681 agttcggtca ctggtacatt cgcttcacga agaatgtccg ccgtagcctg acgagctcgc
    28741 agatgatccg actgctcatg tggcggggcc tgcaacgaac tgagaaagat taattgctta
    28801 actggtactt cacgtagcgc atcgcggacg ttgagagcca gctggcgctc ctgagcgata
    28861 aaatcgccgc cttcgcccat gctgtgcacc agaaaataga cggtatcgat atcctgcaac
    28921 agggccggaa ggttatccgg ccagctgaga tcgactttat ggcaactgac gtttgccagt
    28981 tgcagctttg caagcctgtc gacatgacgt gccgccgcta ggatctgatg cccttgctgg
    29041 ctgagtgtgc gcaccagatg ctgaccaatg tagccactgg caccgagaac taaaatgcgt
    29101 tgcggcacgt ctctctcctt aacgcgccag gaatgcgcgc cagtgggcgg cgacttccgc
    29161 cagttgttcg cgcgagacgt caagatgcgt caccaggcgg acaatcggcg aggcgttaat
    29221 cagcacgttt ctcgctttca tgtattcgcc taacgcggca gcattttctt ccccgacgcg
    29281 aacaaacagc atattagtgt cctgacgcat cacatccgcg cctgcttcac gcagttgctc
    29341 cgccatccag gcggcgttgt cgtgatcttc ctgcaaccgt gcgacgttat tttccagggc
    29401 atacatcccg gcggcagcca gaatcccgga ctgacgcatc ccgccaccgg ccattttccg
    29461 ccagcggatg gcacgtttaa tgtaatcacg attaccgacg agtaatgaac cgactggcgt
    29521 cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa tattgcgtga tctctttcag
    29581 ttcgcagccg taagccacca cggcattaaa gatgcgcgca ccgtcaacat gcagcgccag
    29641 attgcgctcg cgggtaaatt cccatgcttc tttcaggtat tcacgcggca gcactttgcc
    29701 gttgtgggtg ttttccagac tgagtaattt ggtgcgggcg aaatggatat cgtcgggttt
    29761 gattttcatc gccactttat ccagcggtag cgtgccgtcg gcagccgcgt cgatgggttg
    29821 cggctgaata ctgcccagca ccgctgcgcc accggcttca aacagatagt tatgcgcggc
    29881 ctgaccgaca atatactctt cgccacgttc gcagtgactg agcagagcga ccaggttggc
    29941 ctgagtgccg gtaggcagaa aaatggcggc ttctttaccg gaaagctctg ctgcatagtc
    30001 ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg tccccaaccg gggcggccat
    30061 catcgcttcg agcatggcgc ggctcggtcg ggtaacggta tcactgcgta aatcaatcat
    30121 ggcatgtcct tattatgacg ggaaatgcca ccctttttac cttagccagt tcgttttcgc
    30181 cagttcgatc acttcatcgc cgcgtccgct gataattgcg cgcaacatat acaggctaaa
    30241 acctttggcc tgttcgagtt tgatctgcgg tggaatggct aattcttctt tggcgaccac
    30301 cacatccacc aacaccggac cgtcgataga gaaggcgcgt tgcagggctt catcaacttc
    30361 agaggctttt tctacacgga tacccgtaat gccgcacgct tcggcaatgc gggcaaagtt
    30421 ggtgtcgtgc agctcagtac cgtctgtcag gtaacctccg gccttcatct ccatcgccac
    30481 aaagcccagc acgctgttat taaagacgat aattttcacc ggcagtttca tctgcactac
    30541 tgagaggaaa tcgcccatca acatgctaaa accgccatcg ccgcacatgg cgaccacctg
    30601 acgttccggc tcggttgcct gcgcacccaa cgcctgcggc atggcgttag ccatcgaacc
    30661 gtggttaaac gaacctaaca ggcgacgctt gccgttcatt ttcagataac gtgccgccca
    30721 taccgttggc gtgccgacgt cacaggtaaa gatggcgtca tcggcggcaa aatgactaat
    30781 ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc ggtttagcta aatcgtccag
    30841 ccctttgcgg gcatcgcggt aatcttccag cgctttatcc agaaacttgc gatcggtttt
    30901 ttcttccacc agtggcagca atgcgcgcag ggttgatttg atatcgccga ccagcgccat
    30961 atctaccttg ctatgcgcgc cgatgctggc tggattaata tcaatctgaa taattttggc
    31021 atcggtcggg tagaacgcac ggtaggggaa ctgagtaccg agtagcacta acgtgtcggc
    31081 gttcatcatg gtatggaaac ctgacgagaa tccgattaac ccggtcattc caacatcata
    31141 cggattatcg tattcgacat gctctttacc gcgtagggca tgaacaatag gtgctttaat
    31201 tttcccggca aactcaacta actctttatg cgcccccgcg cagccgctgc cacacatcag
    31261 ggcgatattg ctggaataac gcagcagttg cgccagtttg cgtaactctt cttcttccgg
    31321 catcacgact ggctgtggcg catgatacca gtgcgtggtt gccccttctg gcgcaggttt
    31381 taacgccacg tcgcccggta atacaacaac ggaaacgcca cggttaagca ccgctttgcg
    31441 catagcaatt gccagcactt gtgggatctg ctccgggctg gaaaccagct cgcaatagtg
    31501 actacattcg cggaatagct cttgtgggtg ggtttcctgg aaatagccgc tgccaatttc
    31561 gctggaggga atatgagcgg caatcgccag taccggaacg ttattgcggt ggcaatcgaa
    31621 taggccgttg atcaagtgca ggttgcctgg gccgcacgat ccggcacaga ccgccagttc
    31681 tcctgaaagt tgtgcttcag cgccagcggc aaacgccgcc acttcttcat ggcgggtgga
    31741 catccactcg atggtgccca tgcgattaag actgtcgcta agaccgttca gagagtcgcc
    31801 cgtgactccc cagatgcgtt tcacgcctgc cgattcgagt gttttggcga tataagctgc
    31861 aaccgtttgt ttcatggttc tccatctcct gaatgtgata acggtaacaa gtttagttca
    31921 tctgacggag ggggaaggga tgggagagaa aggaggcact aacggttaaa tagcccgatg
    31981 aaaggaatat catcgggcat aaggcgatta tgcgagaacc aaatccccct gcggatggca
    32041 ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg gtcagcgtca ttgtactgct
    32101 caccgtatat tcaccggaaa ccacttttgt cttacagcag ccgcaaacac ccgcacggca
    32161 ggcagcgaca accggaacgt tattgctttc cagcgcctcc agtagcgtgg tgccaaccgg
    32221 ggcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc agaccgctgg tcgccgcttc
    32281 tgctactggg gtgaagaatt tctctttaaa gaaacgcgtc acgccgagcg ctttcacttc
    32341 ctgctctacc caatccatat acggagccgg gccgcaggtc atcacggtac gtgaagctaa
    32401 gtcaggtaca tctgccagca gttcgcgagt gaggcgacca gcgataaagc cttcggtaac
    32461 gttattttct gccaccagcg ttaccggata gttacgccac tcatcggcga aaataacatc
    32521 ctgcggcgta cgcacgttgt agatcacccg cacatcggcc tgtggacggt tcttcgcaag
    32581 ccagcgacgc atcgacataa tcggcgtgac gccgcagcct gccgccagca acaggaattt
    32641 atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg tccgaaagcc agagataatc
    32701 accgcgtttt acatcgcgcg tcagccactg ggagccgaca ccgtcatcaa tccgccgcac
    32761 ggtcagggtg atatattcac tcacgcctgg cgtggaggaa atggtgtaag cacgcagcgt
    32821 ttccgctgag tttcgcacgc tgaccagtgc atattgcccg gcgcgatatg ggtagtgatc
    32881 gtggcaaatc agggaaatcg tccacacatc cggcgtttct tgcgtaatgt gatgaacctg
    32941 tatccgccac gggcattgat tcgttggcat cgtcatcgac aaactcctta cgcgttcaac
    33001 agttgcttca tgtcttcttc aacagtggtg atagaacgca ggccgaattt ctcgttcagc
    33061 accgccagca ggtctggtgt caggaaacca ggtgcagtcg ggccggtgac gatatttttc
    33121 acgcccagag aaagcagcgt cagcagaatg acaatcgctt tctgttcaaa ccaggagagc
    33181 accagcgaca gcggcagatc gttcacacca cagcccagtt tctctgccag agtgacagcc
    33241 agaataattg ctgagtaagc atcgttacat tgacctgcat ctaccagacg cggcagacct
    33301 tcgatatcgc caaactcaag tttgttaaag cgatatttac cacaggcgag ggtcaggatc
    33361 aggcagtcat ccggcacgct ggtggcgaaa tcggtgaagt agtggcgctc gccgcgtgcg
    33421 ccgtcacagc caccaagcag gaagatatga cgcagttttt cacggctaac cagatcaatc
    33481 agcgtatcgg ctgcaccaag cagcgtctgg cgaccaaagc ccacggtgat caggtgagga
    33541 atttcgctgt acgggaagcc cgccatttgt tgcgcctggg cgatgaccgc agagaaatct
    33601 tcgccatcca gatgacgcac gccaggccag ccaacgatgc tgcgggtcca gatacgatcg
    33661 tcataagcgc ctacggttgg gtcgatgatg cagttcgagg tcatcacgat ggggccaggg
    33721 aaacgagcga actccacttg ctgattctgc cagccgctgc cgtagttacc gaccagatgc
    33781 ttgaatttac gcagctccgg gtagccatgc gcaggcagca tttcgccgtg ggtgtagaca
    33841 ttaacgcccg tgccttcggt ctgttccagc aggttgtaga gatctttgag atcgtgaccg
    33901 gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga cctgggttgg cgtcgggtga
    33961 ccgtatttac cggtttcgcc tgcatccaga atgctcatca ctttgaagtt catctggccg
    34021 atttccattg aacactcaag aagcgcgttc atatcggcag gccaggtccc cagccacgcc
    34081 atgattttat ggtactgggc ataaatatcg ttgtcgtatt gaccgagaac atgcgcgtgt
    34141 tccatatagg ccgccgcacc tttcaggcca tacaggcaca gcagacgcag gccgagaatg
    34201 ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt ctgctgcctg acgttgcagc
    34261 tcgccgagat catcgctcac cagttgcagg tcagccatcg ggttatcgac gcgcgcgttg
    34321 gcatctacag ccaggcattg tgctttcagc gcctcgcgca aggcaatcgc ttcacgagcg
    34381 tagccgacaa tacgcggaga atcgaagtta acgttggtca gggttgagaa aaaggcacgt
    34441 ggcgcgaagc tgtcaacatc gtggttgatg atgccgtatt cacgcgcttt taccgcccag
    34501 gcagaaagcc cttgcagcgc cgcgatgagt aaatcctgaa ggtcagaagt ttccgccgtt
    34561 ttaccacaca tcccctgcgc gtatgagcag ccgttttctg ccggagtacg gatagtttgt
    34621 tcacattgca cacaaaacat gatcacacct tttaaagtta tatttaatat acatgtttaa
    34681 gggtaagacg cttaacgcgg ggataaaagg gatttttcat gcaactttaa gggagattga
    34741 tttagcgcaa ttttggcggc agggaactac cgccagagag gtattacgca gagaaaaagg
    34801 cgatgaggat cggcactaac aggctaagaa taaaaccgtg aacaattgcc gccgggacca
    34861 tatccagccc gccagtacgt tgaagaacgg gcagggtgaa atccattgat gtggcaccgc
    34921 ataagcccag tgcagtagag cggctgcggc gaatcagccc agggatcaac ataatagcaa
    34981 tcagttcacg ggccagatca ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt
    35041 cggtcaataa aataccggaa agagaatacc agccgaaacc ggaggccatt gccagcgcgg
    35101 tattgatggg gagatcaaga ataaaggcgt taattaaacc accaattaat gaactgacaa
    35161 ccaccaccac ggcgacaatc attccccggc gattaaggac aatctgcttt aaggtcatgc
    35221 cattattgcg caactgaata ccaacgagga aaagtagcaa aattaacgtg tattcactgg
    35281 cttcggtcgc gtgttgtaag aaagccagtc cactcagacc aatggcaaaa ccaatcacta
    35341 ctacgccgca cagttttagc gactccagcg ccatcgcaat acgcgacggg agtttttctt
    35401 gctgatggtg gttgcgccac ggcaggcctc gctccagcca catcagggcg gcaatattac
    35461 acagtaaaat a
//
LOCUS       DAJTNP010000032        35113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTNP010000032 DAJTNP010000000
VERSION     DAJTNP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="OO160_001749"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="OO160_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5523905.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1209..1871
                     /locus_tag="OO160_001750"
     CDS             1209..1871
                     /locus_tag="OO160_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5523906.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="OO160_001751"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="OO160_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5523907.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /locus_tag="OO160_001752"
                     /pseudo
     CDS             2405..2759
                     /locus_tag="OO160_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2797..3102
                     /locus_tag="OO160_001753"
     CDS             2797..3102
                     /locus_tag="OO160_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5523908.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="OO160_001754"
     CDS             3105..3509
                     /locus_tag="OO160_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5523909.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="OO160_001755"
     CDS             3499..3798
                     /locus_tag="OO160_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5523910.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="OO160_001756"
     CDS             3984..4280
                     /locus_tag="OO160_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5523911.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="OO160_001757"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="OO160_001757"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5523912.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="OO160_001758"
     CDS             5629..5997
                     /locus_tag="OO160_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5523913.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="OO160_001759"
     CDS             complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="OO160_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5523914.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="OO160_001760"
     CDS             7205..7906
                     /locus_tag="OO160_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5523915.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /locus_tag="OO160_001761"
     CDS             7929..8093
                     /locus_tag="OO160_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523916.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /locus_tag="OO160_001762"
     CDS             complement(8305..9615)
                     /locus_tag="OO160_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5523917.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="OO160_001763"
     CDS             complement(9643..10974)
                     /locus_tag="OO160_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5523918.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="OO160_001764"
     CDS             complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="OO160_001764"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5523919.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12685..13074)
                     /locus_tag="OO160_001765"
     CDS             complement(12685..13074)
                     /locus_tag="OO160_001765"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5523920.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="OO160_001766"
     CDS             complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="OO160_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5523921.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="OO160_001767"
     CDS             complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="OO160_001767"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5523922.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="OO160_001768"
     CDS             complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="OO160_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5523923.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="OO160_001769"
     CDS             complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="OO160_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5523924.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="OO160_001770"
     CDS             complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="OO160_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5523925.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20219..20563
                     /gene="tdcR"
                     /locus_tag="OO160_001771"
     CDS             20219..20563
                     /gene="tdcR"
                     /locus_tag="OO160_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5523926.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20819..21359
                     /locus_tag="OO160_001772"
                     /pseudo
     CDS             20819..21359
                     /locus_tag="OO160_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21381..21728
                     /locus_tag="OO160_001773"
     CDS             21381..21728
                     /locus_tag="OO160_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523927.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21897..22547
                     /locus_tag="OO160_001774"
     CDS             21897..22547
                     /locus_tag="OO160_001774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523928.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(23055..23431)
                     /gene="rnpB"
                     /locus_tag="OO160_001775"
     ncRNA           complement(23055..23431)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO160_001775"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23464..24609)
                     /gene="garK"
                     /locus_tag="OO160_001776"
     CDS             complement(23464..24609)
                     /gene="garK"
                     /locus_tag="OO160_001776"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5523929.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24706..25596)
                     /gene="garR"
                     /locus_tag="OO160_001777"
     CDS             complement(24706..25596)
                     /gene="garR"
                     /locus_tag="OO160_001777"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5523930.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25626..26396)
                     /gene="garL"
                     /locus_tag="OO160_001778"
                     /pseudo
     CDS             complement(25626..26396)
                     /gene="garL"
                     /locus_tag="OO160_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26412..27746)
                     /gene="garP"
                     /locus_tag="OO160_001779"
     CDS             complement(26412..27746)
                     /gene="garP"
                     /locus_tag="OO160_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5523931.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28121..29692
                     /gene="garD"
                     /locus_tag="OO160_001780"
     CDS             28121..29692
                     /gene="garD"
                     /locus_tag="OO160_001780"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5523932.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29744..30568)
                     /gene="agaR"
                     /locus_tag="OO160_001781"
     CDS             complement(29744..30568)
                     /gene="agaR"
                     /locus_tag="OO160_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5523933.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30817..32097
                     /gene="kbaZ"
                     /locus_tag="OO160_001782"
     CDS             30817..32097
                     /gene="kbaZ"
                     /locus_tag="OO160_001782"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5523934.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32120..32593
                     /gene="agaV"
                     /locus_tag="OO160_001783"
     CDS             32120..32593
                     /gene="agaV"
                     /locus_tag="OO160_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5523935.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32604..33383
                     /locus_tag="OO160_001784"
     CDS             32604..33383
                     /locus_tag="OO160_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5523936.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33373..34182
                     /locus_tag="OO160_001785"
     CDS             33373..34182
                     /locus_tag="OO160_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5523937.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34269..34703
                     /locus_tag="OO160_001786"
     CDS             34269..34703
                     /locus_tag="OO160_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5523938.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34700..>35113
                     /locus_tag="OO160_001787"
     CDS             34700..>35113
                     /locus_tag="OO160_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5523939.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9095 a         9062 c         8389 g         8567 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagacttt aaaataacgg agatggataa gaattttcta ctaaattaat
    11161 cacaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
    11221 cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg gccagtcctc
    11281 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
    11341 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt
    11401 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
    11461 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
    11521 cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca
    11581 cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc attttataca
    11641 gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac
    11701 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
    11761 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt
    11821 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa accagctgac
    11881 gacgcagcgc tacggcacga cacggcacat tgagcggacc aggcagcacg ccttcagtgt
    11941 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
    12001 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
    12061 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg
    12121 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
    12181 agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
    12241 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
    12301 caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac tctataaatg
    12361 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgataatgg
    12421 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
    12481 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
    12541 cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg aaaatatcga
    12601 atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct
    12661 tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt caatttccag
    12721 cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg
    12781 atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
    12841 gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc
    12901 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
    12961 ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
    13021 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
    13081 tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc tgttcacggg
    13141 tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
    13201 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
    13261 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
    13321 ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga
    13381 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac
    13441 ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac
    13501 gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg atagtcagaa
    13561 tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc ttcataaagc
    13621 gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg tactgcggat
    13681 attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt ggtttcacgc
    13741 gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg atgccgcatg
    13801 ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg tagctgtact
    13861 tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc agccagtcca
    13921 tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc atcagcggtg
    13981 ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta attgcgtaga
    14041 gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta ccaatcacca
    14101 ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat
    14161 cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca tattttttga
    14221 aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt gccggcccca
    14281 tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca
    14341 gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa
    14401 attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt
    14461 cctgtgcctg ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca
    14521 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
    14581 gataagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat
    14641 caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca
    14701 gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga
    14761 gatcggcaaa ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc
    14821 ggcgatagtc accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc
    14881 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
    14941 ttttacgcag atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt
    15001 ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg
    15061 catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag
    15121 cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga
    15181 tacggatgcc ttccattacc ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
    15241 gaaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
    15301 tttcgctttt ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc
    15361 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
    15421 caaattctgc gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt
    15481 cttcgttagt cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct
    15541 cgccaaaaga gttagaacga ttattcattt ctgtatcaat ctctacgcct aatacagcca
    15601 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
    15661 taattccatc cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt
    15721 gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca
    15781 gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta
    15841 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgcaccaa
    15901 agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
    15961 aggtgtcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
    16021 ccacaaccag gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa
    16081 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
    16141 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
    16201 ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
    16261 ttgcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    16321 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    16381 aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    16441 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    16501 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacaatcg ctggcatcga
    16561 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    16621 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg
    16681 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    16741 gacggcgctt tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt
    16801 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    16861 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    16921 tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag
    16981 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    17041 gtaatcgcga acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa
    17101 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg
    17161 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    17221 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    17281 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    17341 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    17401 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    17461 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    17521 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    17581 gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    17641 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    17701 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    17761 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    17821 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    17881 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    17941 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    18001 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    18061 gaaataatac tgacggtttt tctattttga atatattggt ctaatttacc gcttaataat
    18121 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    18181 atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga
    18241 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    18301 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    18361 tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    18421 ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct
    18481 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    18541 gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg
    18601 aagttatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat
    18661 tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc
    18721 gaaacccctt gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca
    18781 tcggtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc
    18841 atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    18901 ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca
    18961 atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata
    19021 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa
    19081 aaacaaacaa ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat
    19141 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    19201 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    19261 tagaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    19321 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    19381 ttctttgtaa cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc
    19441 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    19501 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    19561 ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg
    19621 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    19681 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    19741 ccaccgcttc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    19801 tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt
    19861 tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    19921 ccggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt
    19981 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    20041 ttaattacct cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac
    20101 cactcacaaa ttaactttca ttactttcca aggtatcgtt attacaatta aacatcttta
    20161 aatatcaaca agttaaagca taaaaatcag cataaaccct gatttttatc aaaaaaaaat
    20221 gacggggata accatatttt atggcgataa catcattcgt tatgtggtta atacaaaaaa
    20281 ggggctgaga ccatatttca agcaattacc ggataattac caggcaaaat ttgagttaaa
    20341 tttaatgagc aagttctcaa attttattat aaataaacca ttttcagcga taaatactgc
    20401 ggcacgtcac attttttcac gctatttgtt ggagaacaaa catttatttt atcaatattt
    20461 taaaatttcg aatacaggta ttgatcatct cgaacaattg attaacgtca actttttctc
    20521 ttctgacagg acgtcatttt gtgaatgcaa tcgttttcca taaattcttc tcccctcata
    20581 ggcgacgaat agcattttgt gttgaggatc acaaaacgaa taattgctgg tcgccgcgat
    20641 aaggtcagac gaagtcaaca agggggattt tcactgtgct ttacatcagc gtaggacaca
    20701 gaatgttgct tcgtttactg taacgccggg taaatgagcg ttttttgata gtgcgtaagt
    20761 gtcgtgcgag gaaaataagc atctattatt gtttgtcatg acgacaaggc aggcatcaat
    20821 gaaaggcttc ccaattgcac atatttttca cccttcaatc ccgccaatgc acgcagtggt
    20881 taacaatcac aatagaaata ttgattattg gacggtaaaa agaaagtttg cagaaattgt
    20941 ctccaccaat gacgttaata aaatttacag tataagtaat gaactccgga gagtattatc
    21001 tgcaattact gcattgaatt tctatcaagg cgatgttcct tctgtcatga ttccgaatcc
    21061 aaccggaaaa tatgagtcca ttcatcatag atatttctac aggagaacat gatgattata
    21121 tcatacaaac attagatgta ggcacttttg caccttttgg tgaacaatgt acttgctcag
    21181 ccgtcaataa aaaagagctg gaatgtatta aggagacgat ttctaagtat tgcgcaaagt
    21241 tcacccaaaa agaagccatc ttaacacccc ctaggcactt taataaaacc tgcattactt
    21301 cagattgttg gcaaattctc tttttttcac ccgatcattt taataatgat ttttattgat
    21361 tatttataaa ggataaaata atgttccctg tttcatcaat tggtaatgat attagtagtg
    21421 atttagtccg tcgaaaaatg aatgaccttc ctgaaagccc cataggaaat aacctcgaag
    21481 cactagcacc aggtatagaa aaactaaaac agacctctat tcaaatggtc actttactta
    21541 acgcgttaca accaagtgga aaatgcatta tcactggtga ttttcaaaaa gaattagcgt
    21601 acttacaaaa tgtaattctt tataatgact cgtctcttcg tctggatttt ttaggttata
    21661 acacccaaat tattcaacga tcggacaata cctgcgaact taccattaat gaaccgttaa
    21721 aaaactagga aatatctaca ggcaatatca atgttaattg cccattaaaa gatatttaca
    21781 atgaaatcag gaggttgaac gtaatcttta gttgtgggac tggagatata gttgatctat
    21841 cctctctgga cttacgtaat gtcgatttag attattatga tttcacagat aaacatatgg
    21901 ctaatactat tctaaatcat tttaaattgg attcaacaaa ttttactaat gccaacatgt
    21961 ttcaggttaa ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa
    22021 aaataacgcc tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg
    22081 tcgaaagttg tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat
    22141 ttgcaattat aaaatctata ccaagggcaa ctcttacaga taaattagaa aatgaattaa
    22201 caaaagaaat atataaaagc tcatcgaaaa tcaacaattg cttgaacaga attaaattac
    22261 cagagatgaa agaattctca tcaaaaaaaa catatgatta catcgataca atcattgaag
    22321 attatgaaaa tatcaaagaa aatgcttatc tggtcgtccc ccaaattaat tatactatgg
    22381 atttaaacat agaagactct agctcagaag agttaatacc ctcgagaaag acgaaaattc
    22441 tccggacaat ggctttgagg tcggggaata taacacatat gaagcatata actcagagaa
    22501 tcaatatttt accagagagg actatacgta taattacgac cttttaaatg caatatagac
    22561 ttaataactt cttacaaagt ctattattaa taccgtagat attcatctta ccctgtgcac
    22621 caaaagcaaa aacttatcga cgcgggtttg aaactgatgg caaccgcaaa aatgcctgat
    22681 gcgctacgct tatcaggcct acgccatctc tgcaatatat tgaatttgcg tgcttttgta
    22741 ggcaggataa ggcgttcacg cagcatccgg catgaataaa gcgcactcaa caatctgaaa
    22801 cccgccggaa cggtttatta cgtacatcag gtgaaactga ccgataagcc gctttctttt
    22861 gggtatagtg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa aaacccgccg
    22921 aagcgggttt ttacgtatat caggtgaaac tgaccgataa gccgctttct tttgggtata
    22981 gcgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg
    23041 tttttacgta aaacaggtga aactgaccga taagccgggt tctgtcgtgg acagtcattc
    23101 atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt acgggccgta
    23161 ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac ggactgttac
    23221 cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg cttgcacggg
    23281 ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg cgttacctgg
    23341 caccctgccc tatggagccc ggactttcct cccctccacc cgtctccccc gaagaggacg
    23401 acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt ttgcgcgccc
    23461 ttgtcacccc gtgttgcgca ttccaatcgc cagtgtcgcg gcaatattac gtgaagcacg
    23521 gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc tggtcaatac
    23581 gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg tcaggctacc
    23641 cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcgacgc caatcggtac
    23701 cttgccgtga atactctggc tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc
    23761 gtgaatatgt tcctccagat tcagcgccgt agtgacgatt tcaataccac ttttcagttc
    23821 cgcaccaaga aacgccatca gcgccgcacc cataccgccc gcagctcctg cgccggggac
    23881 atcttttaca tcaacatgca gcgctttttt aatgacatcg gcatagtgag agaggttatt
    23941 gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga tgcgcgatgc
    24001 gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga cgcaatcttt
    24061 taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac taccgccgcc
    24121 aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct gtaccatgcc
    24181 tgcgccgcca tcatttgtag cgctaccgcc aatgccgata ataatgtttg tcgcgccgct
    24241 ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga gtggatcgcg
    24301 tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa acgcggtttt
    24361 gccatcgccg gagatccccc aactggcatt cactttctcg cccagcggcc ctgtaaccca
    24421 ggcgtgacgt tcagaaccct gggtggctgc aatcatcgct tccaccgttc cttcgccacc
    24481 gtcggcaacc ggaatagaaa cgtactgtgc atcaggaaaa atttcccgaa atcctttttc
    24541 tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg gggcgattac
    24601 gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct tattcggcct
    24661 ggattccagg cccggattgc aatacgccat ccgggcacga cgtcattaac gagtaacttc
    24721 gactttcgcc agtttttcgt agtagcacac cagggcgcta tgatccgccg ttcctaaacc
    24781 atctgctcgc agtgcctgca tcatctccat aaccgcagct gtgagcggca gttgcgcgcc
    24841 gacgccgtgg gaagtatcca gcgcatttgc cagatcctta atatgcagat caatacggaa
    24901 gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca cggtactgcc
    24961 cgccagtcca ccgcgaattg cctgatacac caggtccggg ttaacgcccg ctttagttgc
    25021 cagcgttaac gcttctgaca tcgcggcaat attcagcgcc acaatgacct gattcgccag
    25081 tttggtgacg ttacctgcac caattgcccc ggtatgcacc acggaacccg ccattgcttt
    25141 catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca ctgacagcgt
    25201 accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat caatgccttt
    25261 cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac tcatatcgat
    25321 caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg ccacctcttt
    25381 cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag cgatcgcttt
    25441 agccgtagag gctgtttctg cacctgcagc aatcacgtca gcaatagctt ctgggttacg
    25501 gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg gtttacccat
    25561 aatccccagg ccaataaaac caactttcat agtcatatca atcatctctc ttgttgcggt
    25621 ggtggttatt ttttaaaggt atcagccagt ttatgagtgg cagagcggaa gacgccgaga
    25681 tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc atcggcttcg
    25741 accggcgcga ggataccgct gggtttgccg tgcgcgctgg cacggttaaa aatgtgctga
    25801 attgcttttt gtacatccgg gtgtgatgca ttgccgagat ggcctaatgc cgcggccaga
    25861 tcgctggggc cgacgaagat gccgtctacg ccttcggtag cggcaatggc atcgacgtta
    25921 tctacgccct gctgactttc tatctggacc agaatagtga tgttcttgtt cgactgagcg
    25981 aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac gccgcgaatg
    26041 ccttccggtg ggtaacgggt tgatgccacc gcctgctctg cttcctcttt tgtttctaca
    26101 aaaggaatca ggaagttata gaaaccgata tccagaagac gcttaataat ttccggctcg
    26161 ttggtcggca ctcacactac tggcgcgctg gcgctgcctt tcaaggccat taactgcgga
    26221 ataaacgtgg agatatcgtt tggcgcatgt tcgccatcca gcaccagcca gtcaaaccca
    26281 gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca accaatttgt
    26341 acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt attcatcgct
    26401 tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac gaagaggtag
    26461 cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt gaaggagtgc
    26521 agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac attgccaaag
    26581 acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc agaaatcacc
    26641 ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag catgaccacc
    26701 agcgtggtgt tgttggtgta gttacataag atgatggtgg aagccagcaa cattcccagc
    26761 acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag ataatccgag
    26821 aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga ggcgaccaga
    26881 cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa ccaggtgagg
    26941 aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa catcatgcgg
    27001 ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc gctgcccggc
    27061 tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag ctcttcctca
    27121 gacatacgtg ggtgatctgt cgggttatga atcaacttga tccacagcgc cgtcagcaca
    27181 aaacaaatca cccccataac ggtaaagacg tgctcccatc cccaggcgaa agtcagccag
    27241 ccaagcagag gcgaaaagag cgccagcgag aaatattgtg ccgagttaaa gatggcggag
    27301 gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt cgccgggaag
    27361 gatggagctt ccgagaatcc cagcataaag cgcataaaga acatggagat ccctgcccaa
    27421 gccagcggga acatatcaac aaagccttgc aggaaggtga acagcgacca gaaaaagagg
    27481 ctgtaggtgt aaactttttt cgagccaaac ttatcaagca accagccgcc ggggatttgc
    27541 atcagcaagt aggcccagcc aaaagcggag aagatgtaac ccatcgaaac cgcacttaac
    27601 tgcaactctt ttgccacttc ggtaccagca atagacagcg ttgcacgatc ggcgtagtta
    27661 acggcggtaa caataaaaat aatcagtaat attaaatagc gggtatgcac acctttcctt
    27721 ttttcgtcaa cggtgtccag aatcatttta tttacctcgg gtacttatgc tgatttttat
    27781 tattatgggg aaggtgttat ttataagttt catttatgcc gtaacgacaa tgaactcggg
    27841 aattagtata agcagcgcga gaataataat cattgtgcaa atgctaattt aattaatact
    27901 ttttaaatat tattttgagc atatgcacat aaggttgcgc gctaaagcac agatttgcgc
    27961 tttaccttgc cgggcggcac tgcaatccct aaaatgattg acattgatca catttctgcg
    28021 tttaaactcc tgacattctt atttcaccca atgaaatcat ttatttttaa atgagaccag
    28081 gtcctcattt taataacccc tggctggaga atattgcaca atggccaaca tcgaaatcag
    28141 acaagaaacg ccaactgcgt tttatataaa agttcacgac acagataatg tggcaattat
    28201 tgttaatgat aatggcctga aagcaggaac gcgttttccg gatgggctgg aattaattga
    28261 acatattccc caggggcata aagtcgcatt gctggacatt ccggctaatg gtgaaattat
    28321 tcgttatggc gaagtgattg gttacgccgt gcgtgcaatc ccacgcggaa gctggatcga
    28381 cgaatcaatg gttgtactac cggaagcgcc gccgttacac acgctgccac tggcaaccaa
    28441 agtcccggaa cccttaccgc cgctggaagg atacaccttt gagggctatc gcaatgccga
    28501 tggcagcgtg ggcaccaaaa acctgctcgg catcaccacc agcgtccact gtgtggcagg
    28561 tgtggtggac tatgtagtaa aaatcattga acgcgatctg ctaccgaaat acccgaacgt
    28621 cgatggcgtg gtggggctga atcatttgta cggttgtggc gtggcgatta acgcaccggc
    28681 agcagttgta cctattcgta ccattcacaa tatttcgctg aaccctaact ttggcggcga
    28741 agtaatggtg attggcctgg gttgtgaaaa gttgcagcct gagcgcctgc tgactggaac
    28801 ggatgatgtg caagctattc cagtagaaag cgccagcatt gtcagtttgc aggatgaaaa
    28861 gcatgtcggt tttcagtcca tggtcgagga tattttgcag gtcgccgaac gccatctaca
    28921 aaaactgaat caacgtcagc gagaaacctg tccggcttca gaactggttg tcggcatgca
    28981 ttgcggtggc agcgatgcgt tttctggcgt aacggcaaac ccggcggttg gctatgcgtc
    29041 tgatctactg gtgcgctgcg gcgcaacggt gatgttttca gaagtcacgg aagtgcgtga
    29101 cgcgatccat ctgctgacac cacgcgcagt gaacgaagag gtcggcaaac ggctgctgga
    29161 ggagatggag tggtacgata actatctcaa tatgggaaaa accgaccgca gcgccaaccc
    29221 ttcgccgggc aacaagaaag gcggtctggc aaacgtggta gagaaagcgc tcggctccat
    29281 tgctaaatcg ggcaaaagtg caattgttga agtgctgtcg cccggtcaac gcccgactaa
    29341 acgcggatta atttacgccg cgacgccagc cagcgatttt gtctgtggca cgcaacaggt
    29401 ggcttcgggt atcaccgtgc aagtgtttac gaccggtcgt ggtacgccgt acggcctgat
    29461 ggcggtaccc gtcattaaaa tggcaccccg caccgagctg gcgaaccgct ggtttgattt
    29521 aatggatatt aatgcgggca ccatcgctac cggcgaagaa actattgaag aggagggctg
    29581 gaagttgttc cactttattc tcgacgtcgc cagcgggaag aagaaaacct tctcggatca
    29641 atggggactg cataaccagc tggcggtgtt taacccagca ccggtgacct gataattcaa
    29701 ttcgtattat gcccgatagc acacgtttat cgggcatatt gagtcacagc gaagacgcgt
    29761 gatccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga ctatccgcag
    29821 gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc ttatgtaaac
    29881 tggaacgatt gaatttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca
    29941 tccgacggct taaacgggct tcatcttcat tatgtgtgct gacgccgcgc tccagatcga
    30001 tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct
    30061 gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc agcagctcaa
    30121 cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt
    30181 cagtgtactt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa tcgaggatca
    30241 cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg
    30301 tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg ctatcgcaga
    30361 tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac gccagatcat
    30421 tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc
    30481 cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcct gtcactcgct
    30541 tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt
    30601 tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg cccattggcg
    30661 caagctactc tcgtttcagt gactttcatt acgtttcttt tgtgaatcag atcagaaaac
    30721 tattatcttt cgttttattt ttatctcacc atgacgcagt atcaattgaa acaaaacgaa
    30781 agatcaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac agaaatggtg
    30841 agacagcaca aagcgggcaa aacaaatgca atttatgccg tttgttccgc acatccgctg
    30901 gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact gattgaagca
    30961 acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc cgattttcgc
    31021 ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgct gattctgggt
    31081 ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc aatggccaat
    31141 gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca ccttgattgc
    31201 agcatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc tgaacgcgcc
    31261 gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga agccgatctg
    31321 gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac cttaagcgag
    31381 ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg tcacgccttt
    31441 gaaaagcaag gtttgaatgc catctggcca cgcattattg ccctggtggt tcaacccggc
    31501 gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgag cgccttaagc
    31561 cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga ttatcaaacg
    31621 ccgcaatctc tgcgccagct ggtgattgac cactttgcca ttctgaaagt tggcccggcg
    31681 ctgaccttcg ccctgcgtga agcccttttt tctctggcgg cgattgaaga agaactggta
    31741 ccagcgaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct cgaccgcccg
    31801 gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttac
    31861 agctactcag atcgcgtgcg ctattactgg ccggacagcc agattgatga cgctttcgct
    31921 catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag ccagtatctg
    31981 ccgctgcagt acgtgaaagt tcgctccggc gagctgcaac cgacgccacg ggaactcatt
    32041 ataaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg ccaataagca
    32101 aaacaaagag gaacatgcta tgccaaatat tgttttaagc cggattgatg aacgcttgat
    32161 tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg tgctggtagc
    32221 caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg tactggcaga
    32281 agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc atcgcgccgc
    32341 cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga cgctggtgaa
    32401 aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca atggcaaaca
    32461 acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta acgacctgaa
    32521 agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg tggacctctt
    32581 taaattactt tgagggattc atcatggaaa tcagcctgtt gcaggcattt gcgttgggca
    32641 ttatcgcctt tatcgctggc ctagatatgt ttaacggctt aacccatatg caccgcccgg
    32701 tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga attttaaccg
    32761 gcgatacgct ggaactagtg tggatggggc tggccccgct ggcgggcgca cagccgccta
    32821 acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc gtgaaacccg
    32881 atgtcgcggt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg attaccttcc
    32941 tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag aacgccgata
    33001 ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact ttctattttc
    33061 tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc attgatgtcc
    33121 tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt
    33181 ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat accctacttc atcctgggct
    33241 ttgttgccgc aacctggctc aagttaccgg tgctggctat tgctgccgcc gcgctggcga
    33301 tggcgctgat cgatctgctg cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag
    33361 aggaatttga agatggcatc taatcaaacc accctgccga acgtcagcga aaatgaagaa
    33421 acactgctga ctggcgtcaa tgaaaacgtg tatgaagatc ggagcattgc cgcggagctg
    33481 acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac
    33541 tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct gaaaaagatc
    33601 cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc
    33661 catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg ttctaagcag
    33721 gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg cgggattggc
    33781 gatgcgatgt tctggctaac gctactgccg atttgtggcg ggataggcgc aagcctcgcg
    33841 ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt ggtacacttg
    33901 gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc gattcccctt
    33961 attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat gacggtaatc
    34021 ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac ggcgggcgac
    34081 gcagtagtta agttacaggc tgatgttatc gacaaactga tgcctgcctt cttaccgctg
    34141 gtctacaccc tgaccatgtt ctggctggta cgccgcggct agagtccgct gcgcctgatt
    34201 gcggttaccg tggttctagg gatcgtcggt aaattctgcc atttccttta aatacaatga
    34261 ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt ggcatggaaa
    34321 aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat tttcccgaaa
    34381 cctcaagcgc cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa ctggattgtg
    34441 aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc gtagcttcaa
    34501 cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg caactgctac
    34561 tggagatggt gctggagcgc gaagggttaa gcggtgaaga attccgtgta caggcgctgg
    34621 agtgtggaca ccgagggctg accagtctgg tggacgagtt aggccgctgc catgaagaat
    34681 gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga
    34741 gggatggttc gatgaccatc agttgcgtgt tgctgacggt gtcatcgcag caatcgaacc
    34801 gattccagtg ggcgtgactg aacgcgatgc ggaacggctc tgccccgctt acatcgacac
    34861 ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg tactcgacaa
    34921 gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca ccgtaaccgc
    34981 gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc aacgcggcgg
    35041 acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc cgcagaataa
    35101 aggcgcgcat ccg
//
LOCUS       DAJTNP010000033        34957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTNP010000033 DAJTNP010000000
VERSION     DAJTNP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..773)
                     /locus_tag="OO160_001788"
     CDS             complement(<1..773)
                     /locus_tag="OO160_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5523940.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNP"
     gene            865..951
                     /locus_tag="OO160_001789"
                     /pseudo
     CDS             865..951
                     /locus_tag="OO160_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1356..1832)
                     /locus_tag="OO160_001790"
     CDS             complement(1356..1832)
                     /locus_tag="OO160_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5523941.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1891..3180)
                     /gene="bioA"
                     /locus_tag="OO160_001791"
     CDS             complement(1891..3180)
                     /gene="bioA"
                     /locus_tag="OO160_001791"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5523942.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3267..4307
                     /gene="bioB"
                     /locus_tag="OO160_001792"
     CDS             3267..4307
                     /gene="bioB"
                     /locus_tag="OO160_001792"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5523943.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4304..5458
                     /gene="bioF"
                     /locus_tag="OO160_001793"
     CDS             4304..5458
                     /gene="bioF"
                     /locus_tag="OO160_001793"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5523944.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5445..6200
                     /gene="bioC"
                     /locus_tag="OO160_001794"
     CDS             5445..6200
                     /gene="bioC"
                     /locus_tag="OO160_001794"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5523945.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6193..6870
                     /gene="bioD"
                     /locus_tag="OO160_001795"
     CDS             6193..6870
                     /gene="bioD"
                     /locus_tag="OO160_001795"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5523946.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7449..9470
                     /gene="uvrB"
                     /locus_tag="OO160_001796"
     CDS             7449..9470
                     /gene="uvrB"
                     /locus_tag="OO160_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5523947.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9662..10570)
                     /gene="yvcK"
                     /locus_tag="OO160_001797"
     CDS             complement(9662..10570)
                     /gene="yvcK"
                     /locus_tag="OO160_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5523948.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10835..10979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10967..11956
                     /gene="moaA"
                     /locus_tag="OO160_001798"
     CDS             10967..11956
                     /gene="moaA"
                     /locus_tag="OO160_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5523949.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11978..12490
                     /gene="moaB"
                     /locus_tag="OO160_001799"
     CDS             11978..12490
                     /gene="moaB"
                     /locus_tag="OO160_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5523950.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12493..12978
                     /gene="moaC"
                     /locus_tag="OO160_001800"
     CDS             12493..12978
                     /gene="moaC"
                     /locus_tag="OO160_001800"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5523951.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12971..13216
                     /gene="moaD"
                     /locus_tag="OO160_001801"
     CDS             12971..13216
                     /gene="moaD"
                     /locus_tag="OO160_001801"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5523952.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13218..13670
                     /gene="moaE"
                     /locus_tag="OO160_001802"
     CDS             13218..13670
                     /gene="moaE"
                     /locus_tag="OO160_001802"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5523953.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13807..14511
                     /locus_tag="OO160_001803"
     CDS             13807..14511
                     /locus_tag="OO160_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5523954.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14716..15429
                     /locus_tag="OO160_001804"
     CDS             14716..15429
                     /locus_tag="OO160_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5523955.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15465..16421)
                     /locus_tag="OO160_001805"
     CDS             complement(15465..16421)
                     /locus_tag="OO160_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5523956.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16421..17662)
                     /gene="clsB"
                     /locus_tag="OO160_001806"
     CDS             complement(16421..17662)
                     /gene="clsB"
                     /locus_tag="OO160_001806"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5523957.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17659..18420)
                     /locus_tag="OO160_001807"
     CDS             complement(17659..18420)
                     /locus_tag="OO160_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5523958.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18553..18963
                     /locus_tag="OO160_001808"
     CDS             18553..18963
                     /locus_tag="OO160_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5523959.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18925..20031)
                     /locus_tag="OO160_001809"
     CDS             complement(18925..20031)
                     /locus_tag="OO160_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5523960.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20042..21175)
                     /locus_tag="OO160_001810"
     CDS             complement(20042..21175)
                     /locus_tag="OO160_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5523961.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21168..22904)
                     /locus_tag="OO160_001811"
     CDS             complement(21168..22904)
                     /locus_tag="OO160_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5523962.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22897..23892)
                     /gene="hlyD"
                     /locus_tag="OO160_001812"
     CDS             complement(22897..23892)
                     /gene="hlyD"
                     /locus_tag="OO160_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5523963.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23895..24566)
                     /gene="cecR"
                     /locus_tag="OO160_001813"
     CDS             complement(23895..24566)
                     /gene="cecR"
                     /locus_tag="OO160_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5523964.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24795..25982
                     /gene="rhlE"
                     /locus_tag="OO160_001814"
                     /pseudo
     CDS             24795..25982
                     /gene="rhlE"
                     /locus_tag="OO160_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26189..26671)
                     /gene="ybiA"
                     /locus_tag="OO160_001815"
     CDS             complement(26189..26671)
                     /gene="ybiA"
                     /locus_tag="OO160_001815"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5523965.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26791..28941
                     /gene="dinG"
                     /locus_tag="OO160_001816"
     CDS             26791..28941
                     /gene="dinG"
                     /locus_tag="OO160_001816"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5523966.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28969..29931
                     /gene="ybiB"
                     /locus_tag="OO160_001817"
     CDS             28969..29931
                     /gene="ybiB"
                     /locus_tag="OO160_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5523967.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30072..31157
                     /gene="hcxB"
                     /locus_tag="OO160_001818"
     CDS             30072..31157
                     /gene="hcxB"
                     /locus_tag="OO160_001818"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5523968.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31386..31646)
                     /locus_tag="OO160_001819"
     CDS             complement(31386..31646)
                     /locus_tag="OO160_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5523969.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31911..32177)
                     /gene="ybiI"
                     /locus_tag="OO160_001820"
     CDS             complement(31911..32177)
                     /gene="ybiI"
                     /locus_tag="OO160_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5523970.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32251..32928)
                     /gene="ybiX"
                     /locus_tag="OO160_001821"
     CDS             complement(32251..32928)
                     /gene="ybiX"
                     /locus_tag="OO160_001821"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5523971.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32970..34957)
                     /locus_tag="OO160_001822"
                     /pseudo
     CDS             complement(32970..34957)
                     /locus_tag="OO160_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8285 a         8965 c         9441 g         8266 t
ORIGIN      
        1 aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat actgtctggc
       61 aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg aagttcgggc
      121 agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg aaggggcggt
      181 agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg taattctggt
      241 aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg aagatcaggt
      301 agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg aagagaaggc
      361 aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg cggaagagcc
      421 gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg agggagttct
      481 ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg gggcaggtat
      541 gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa agccagacat
      601 attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc cttggcccag
      661 ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa acgggatata
      721 catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt catttattat
      781 tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat tctttaaaaa
      841 aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct ggccgaaaat
      901 gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg ttggtcgccc
      961 taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc gcttatcgag
     1021 atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat tagcgcaaga
     1081 ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca tctaagtggt
     1141 tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat tttttaagct
     1201 aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca gcttttttat
     1261 actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat acctttaagt
     1321 gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc ggtaatcgat
     1381 gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct ggctccttca
     1441 tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg atgggtttcg
     1501 cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg cgtctgcaaa
     1561 acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac gcgggtatca
     1621 gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc atccgggtcg
     1681 tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc cagatgcggt
     1741 gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg caacttatcg
     1801 ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt tgcagaaagt
     1861 gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt catcctgtac
     1921 cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa tatagggcgg
     1981 catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga caaagaattt
     2041 ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg cccccagtac
     2101 gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc gcagctgcac
     2161 ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta ggctggcgtt
     2221 tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa agcagccggc
     2281 ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg aaagggtcat
     2341 tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt ctgcatattc
     2401 acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg caatcagcaa
     2461 gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat ggtacatgcg
     2521 catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt catgacgatg
     2581 cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac catccatgcg
     2641 gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca ggctgtgcat
     2701 tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat aaccattgcg
     2761 gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca acgccatttt
     2821 catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact ccagcggttg
     2881 cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg tgataccgcc
     2941 aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa gctgcgggtg
     3001 attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc gtctgccgtc
     3061 agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca gaggggaggt
     3121 catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt ccgttgtcat
     3181 aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc gaattaacaa
     3241 caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca ttgtcgcaag
     3301 tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag caggtgcatc
     3361 gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt aagaccggag
     3421 cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc gggctggaag
     3481 ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg aaagcggcag
     3541 gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc gatatgccgt
     3601 acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt atgacgctgg
     3661 gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat tactacaacc
     3721 acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc acttatcagg
     3781 aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc tcaggtggca
     3841 ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa ctggcaaacc
     3901 tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa ggcacgccgc
     3961 ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc gtagcgcgga
     4021 tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg aacgaacaga
     4081 ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc aaactgctga
     4141 ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg gggctaaatc
     4201 cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt ggacaggcgc
     4261 tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct ggcaggagaa
     4321 aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc gttatccggt
     4381 ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga acttttccag
     4441 taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga agcagggggc
     4501 ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata gcgtggtgca
     4561 tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc tgctgtttat
     4621 ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag aggaccgtat
     4681 tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa gcccgtcgca
     4741 gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg cttccccctg
     4801 tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg atagcgcgcc
     4861 actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg tcgatgatgc
     4921 ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc aaaaggtaaa
     4981 accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg ctgcggtgct
     5041 ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta tctacagcac
     5101 cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca ttcgccgtga
     5161 tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc gtgccggagt
     5221 acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat tgatcgtcgg
     5281 tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct gctgggtcac
     5341 ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa cgctaaccgc
     5401 tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca acggttaata
     5461 aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa catgcagatc
     5521 tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa tacacccacg
     5581 tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa cgtcacgcgc
     5641 aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat aaagatgccg
     5701 cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg acgttcgatc
     5761 ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca ctccgcgagc
     5821 tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg cagggatcgt
     5881 tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct aatcgctttt
     5941 taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat catattcagc
     6001 tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa ggcatcggtg
     6061 ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag ttgcagcgat
     6121 tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat ctttttttgg
     6181 gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga agtggggaaa
     6241 accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg gacggcaggt
     6301 tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa tagtgacgcg
     6361 ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa tccttacacc
     6421 ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc gatagaatca
     6481 ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt gttagtggaa
     6541 ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga ttgggtaaca
     6601 caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat taatcacgcg
     6661 atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg ggtggcgaac
     6721 gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac ccgcatgatt
     6781 cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa tgcggcaacc
     6841 ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa ttgcgagcga
     6901 gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat cacgaaaaaa
     6961 attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg ggataggggc
     7021 tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta gaaaacacga
     7081 ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc tcaaagaaat
     7141 atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca ggcatctctt
     7201 gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc tttggggata
     7261 acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg attgacagcg
     7321 gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc agtataattt
     7381 gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac tccttcaggt
     7441 agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct ggcgatcagc
     7501 cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac cagacgttac
     7561 ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct gaccttcagc
     7621 gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat ggcgaaatga
     7681 aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac tactatcagc
     7741 cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg gttaacgaac
     7801 atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt gatgtgattg
     7861 tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat ctcaagatga
     7921 tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc cgactggcgg
     7981 agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc gttcgtggcg
     8041 aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg gaactgtttg
     8101 acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt gtttccacta
     8161 ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag cgcatcgtac
     8221 aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg ttggaaaaca
     8281 acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg gagatgatgg
     8341 acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc ggtcgtggac
     8401 cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg ctggtcgtcg
     8461 atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac cgggcgcgta
     8521 aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac cgtccgctta
     8581 agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg acgccgggta
     8641 attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt ccaaccggat
     8701 tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat cttctttcgg
     8761 agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg accaagcgaa
     8821 tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt tatcttcact
     8881 cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg ggtgagttcg
     8941 acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa gtgtcgctgg
     9001 tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg ttgatccaga
     9061 ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc gataggagca
     9121 ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa cagcagaagt
     9181 acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc gatatcctgg
     9241 cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg cgcccgattg
     9301 ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag aaaatccatg
     9361 aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa gcggcacaaa
     9421 ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa caggatagcg
     9481 aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag gcctacattt
     9541 tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc acgccgcatc
     9601 cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct tcggatttcc
     9661 gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg cggtcatgac
     9721 gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct ttcaccgccg
     9781 agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca acatactgct
     9841 ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc aactcacgcc
     9901 ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg gcgatttcct
     9961 tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata atgatgagat
    10021 ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc gttaacaata
    10081 actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact tcatgcccct
    10141 gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa tgcgtatcca
    10201 ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta aggtgatcca
    10261 gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg ccgccaaaac
    10321 ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg aggcagttgc
    10381 gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc gagccaccat
    10441 tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt gatgagagaa
    10501 cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca gccagcgtac
    10561 gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc aaattgcggg
    10621 gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta tctctaaagg
    10681 acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca ttttccctta
    10741 ttctgtagcg aaatagcacg atcatgacgc tatatatatg attacatagc gaaagtgtgg
    10801 atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact ctagcctctg
    10861 cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga ttgccaccag
    10921 ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg cttcacaact
    10981 gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg atgtgtgtaa
    11041 ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca ccaataaagg
    11101 ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc tgggcaccga
    11161 aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg atatcatcgc
    11221 cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg gttaccgtct
    11281 ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg tcagtgtcga
    11341 cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca accaggtcat
    11401 ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca ataccgtgct
    11461 gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc agcatcgccc
    11521 tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc tcttccgtaa
    11581 gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct ggatccacca
    11641 attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt acgccggaga
    11701 gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc gcctgcgcgt
    11761 ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta acctgcgcga
    11821 tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag cggcgctgcg
    11881 ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc aaaacttatc
    11941 gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa gcactgaatt
    12001 tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag aagacgatac
    12061 ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg tcgatgaagc
    12121 cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga tcgccagcga
    12181 cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg atcaggctcc
    12241 cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag tgttccgtat
    12301 gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag cgggcgtcgc
    12361 taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta ccgcatggga
    12421 aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc atccacattt
    12481 gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc gcacatggtg
    12541 gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt tgtcaccatg
    12601 cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga cgtatttgcc
    12661 actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat cccgctgtgt
    12721 cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga gcacaatcgg
    12781 gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat ggaagcgtta
    12841 accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt gcaaaaagat
    12901 atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg tgactttaag
    12961 gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg tgggaacaga
    13021 tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc acctggctgc
    13081 gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg ccgtcaacca
    13141 gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt tcttcccgcc
    13201 ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag ccgttcagcg
    13261 taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta gtcaccttca
    13321 ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc ctcgaacact
    13381 atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt aaccgctggc
    13441 cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc gatgaaatcg
    13501 tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg cagtttatta
    13561 tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg gaaggcgacc
    13621 gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag ttttttgtta
    13681 gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg tttacgctgc
    13741 gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata tcttcaggag
    13801 atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc tggcttgcaa
    13861 acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac cgcatttgtt
    13921 gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa ccgtgtcttt
    13981 ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc gatgattcaa
    14041 aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct gacgggtctt
    14101 acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac tttcgtcgtt
    14161 actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg cgatttaagt
    14221 ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc gctggtcaac
    14281 ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt gattgtcttt
    14341 gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca gattgatacc
    14401 cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt gtatctggac
    14461 ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta attctttttc
    14521 accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga cacttaacaa
    14581 gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat ccataaatac
    14641 aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat gttttttcta
    14701 aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga ttttcagcac
    14761 gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt ggcaaccgca
    14821 ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga ttcaccacca
    14881 atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa ggatatcaac
    14941 aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt ggtggcgatt
    15001 gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac tgcgggcgcg
    15061 atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg ttctcaccgt
    15121 tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa tgcggcatta
    15181 atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg gccaggcatt
    15241 atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag tgaagagttg
    15301 tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta ttttatcggc
    15361 ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca tacgcgtttt
    15421 tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat cgccgcttca
    15481 tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat cagcgccagc
    15541 agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat aatgctccct
    15601 ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac accgatcccc
    15661 gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc cagtacaaag
    15721 aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac catccagtta
    15781 acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac cagcttttgc
    15841 cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata aaccgcgata
    15901 atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata ccagtaatcc
    15961 ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata gcccagccag
    16021 ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa ccctagtcgg
    16081 gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt caggttgaag
    16141 gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg accgcagtaa
    16201 aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac cagcccgacc
    16261 gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc ttcccagtcc
    16321 acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag ccaggtgagg
    16381 atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt acccccgtgt
    16441 tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga tcaacctgcg
    16501 tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg cgtaaaaagt
    16561 ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc atggtttcat
    16621 ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc gtctggttaa
    16681 aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg agcggatcga
    16741 gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg ccgtggagcg
    16801 ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta tacagcaagc
    16861 gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt ttgatccgca
    16921 ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg aagaagtagg
    16981 cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa tggcgttcaa
    17041 tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct tctcccggct
    17101 ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg ctctgtccag
    17161 gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca aggcgtaccg
    17221 cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag taattcagcc
    17281 cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc cggcgaaaca
    17341 cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac actacgccag
    17401 ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag ccatccagca
    17461 agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc agctgtttgc
    17521 cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt gcctcgccaa
    17581 tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg atcttattgc
    17641 cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat gatcagaaag
    17701 gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat ttttgacgta
    17761 gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg ggcgtccgtg
    17821 ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat tagctttttg
    17881 ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta gctcattcac
    17941 ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc ccagatgtac
    18001 gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt agagcacgcc
    18061 gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa tgggataacg
    18121 cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac cgtaggcaaa
    18181 atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc agttttccac
    18241 atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa tatcggcgct
    18301 gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa acgcggtaaa
    18361 gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt gatcgggcat
    18421 aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc acaaaaaggt
    18481 gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga taaattcttc
    18541 aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct aagttgctgg
    18601 cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat gagtaagact
    18661 ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt gatgatgctg
    18721 gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct ggaagagctg
    18781 accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc ccgcgtactg
    18841 gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc ggcggtagtg
    18901 tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg cgtacgccgc
    18961 tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca caatatccag
    19021 actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa tagggttaat
    19081 ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt aaccggaaag
    19141 gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt gtgtcgagca
    19201 gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca ccatcgtaaa
    19261 gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac caatcgccag
    19321 cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc cgatgaagat
    19381 ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt cacgttcgcg
    19441 ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg cgatcagcga
    19501 cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta ccaccagctc
    19561 gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt tgacgatctg
    19621 ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga ggatcaactg
    19681 caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga aacgcaccag
    19741 tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt tcagcagcag
    19801 cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg aatgctcgcc
    19861 gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg cggcgaacgg
    19921 gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt gtggttcgcg
    19981 cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca tgctcttctc
    20041 cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc accgccgaag
    20101 cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg aggaacaggc
    20161 tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg cggatcaccg
    20221 cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc agaaaagcgg
    20281 cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa atcagcagcc
    20341 ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc agcagcgaac
    20401 cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac atcgccagca
    20461 tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg gtaatctccg
    20521 tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc agcgaggtga
    20581 gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata aagtgctggc
    20641 ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt tcaaaagtct
    20701 gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg acatacccct
    20761 gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg
    20821 cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga ccgcgaattt
    20881 tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg gcgtcgatgt
    20941 agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc tgttccagca
    21001 aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa ataaacagca
    21061 gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg atctgccgcg
    21121 tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta ctcattgcta
    21181 tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt gggatcgggt
    21241 tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc gattaatttc
    21301 ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa gtgggtggtg
    21361 accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa ttcacggcgg
    21421 gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg ttcatgcatc
    21481 agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc ggtggcgtgt
    21541 gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc gttctgcgcc
    21601 cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac cgtcaggttg
    21661 ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt accggaactc
    21721 tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag caaaccgcac
    21781 atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc aaaaatctcc
    21841 ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc
    21901 agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg caatattgca
    21961 cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc atcttcaaaa
    22021 cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg aatatcgtct
    22081 ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat gccatcgctg
    22141 acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg tggactggtc
    22201 atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc ctgatacagc
    22261 aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc gagatacgag
    22321 gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg ccacagttcg
    22381 cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac tttcggttcg
    22441 cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag cttgcccgcc
    22501 aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa agtttgctta
    22561 cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc catcaccgtg
    22621 agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg cagcgcgccg
    22681 tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg tttcagtaat
    22741 cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac caaccccgtc
    22801 acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc catgcccgga
    22861 aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg cctcgtcacc
    22921 gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg tcaccacaat
    22981 acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt tcggggtaaa
    23041 ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg ggcgaccatc
    23101 ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt catcaacata
    23161 agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac cttcattgag
    23221 aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg ctatcaacgt
    23281 tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt cgaggctggc
    23341 tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca atttatcctg
    23401 tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt ccaggtcatt
    23461 tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt tctgcgcata
    23521 gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt cttcatcgcg
    23581 atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg ctttcgcctg
    23641 catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc ccgctttgat
    23701 agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga aactaagatt
    23761 caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc ggctttgata
    23821 ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc cgatcacgac
    23881 aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag gtcgatatga
    23941 caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt ccagccggta
    24001 cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat caacgcatgg
    24061 gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc aatcaggcgt
    24121 gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta ggctgccgtg
    24181 ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc ctgggtgagc
    24241 agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc ccgatcaggc
    24301 tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg ctcgccaata
    24361 aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag
    24421 gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt ggcgttcatt
    24481 ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc ctgttcaccc
    24541 ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat gattgattaa
    24601 gattatgact ccatatggga gttgtccagt atggctaaga attttagcta cgccagtcac
    24661 agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc atgacattgt
    24721 ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa cggtcggggc
    24781 ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc ctgcgcgccg
    24841 ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc cctgctgtgc
    24901 tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca gcgggcttta
    24961 cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg cgtcgtccgg
    25021 tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc gaaaacgtcc
    25081 gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt gtcagcatta
    25141 acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg ccgggacgtt
    25201 tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc ctcgtcctcg
    25261 atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc gtgttaacaa
    25321 aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac gatattaaag
    25381 ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc cgcaataccg
    25441 cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc
    25501 tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc cgtaccaaac
    25561 acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt gtggcgatcc
    25621 acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa tcgggtgaca
    25681 ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa gagttgccgc
    25741 acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt atcgggcgta
    25801 ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat gaacacaaac
    25861 tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg attccgggct
    25921 atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag caacgtggcg
    25981 gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt
    26041 gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg tttacgccgc
    26101 atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa aaatttatct
    26161 gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt tcgcgcaact
    26221 ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct ccccagtaag
    26281 catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag agcacacgca
    26341 gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt tctttaaccg
    26401 actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg gcgaccatgg
    26461 gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt tgtgcctgaa
    26521 aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg gcggcaaagt
    26581 tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg tcttgcatga
    26641 cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt ggttattgcg
    26701 agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg ccacaatatt
    26761 ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct taaagcgcaa
    26821 attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc ccgtgcgccg
    26881 cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg gcggcatctg
    26941 gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc cggcatcgcc
    27001 attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc gttgcaggat
    27061 cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct taaattcact
    27121 gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct cgccagcact
    27181 gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc aaataatcag
    27241 gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa atgggatggt
    27301 ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt gagtaccgac
    27361 aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt ttttgttact
    27421 cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt gatggcggcg
    27481 atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct ggacgaaggt
    27541 catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat caccgtgccc
    27601 tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat ggagcagttt
    27661 cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca ttgtgaagag
    27721 ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc tgccgggcag
    27781 gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga gatctgccag
    27841 cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct taacgattta
    27901 agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt acagatgaac
    27961 cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc tctggcgcaa
    28021 tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg gcagctacac
    28081 ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct gctgtggcgc
    28141 agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag tttttcgcgt
    28201 ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc gctggattcc
    28261 ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt tgagccttcc
    28321 atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga acaggtggag
    28381 agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat gcagcgcttt
    28441 ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca gccgcgttat
    28501 cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt gctggtgggc
    28561 ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca ggtgcatatc
    28621 cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg ggaatggctg
    28681 aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc gtttaacctg
    28741 attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt ggttatctac
    28801 gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt accggtattt
    28861 ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa aacgaaatcc
    28921 ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat ggactatcgc
    28981 aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga ccgggatact
    29041 gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga gttgggcggc
    29101 gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg cttttacgaa
    29161 gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat gccgattgtc
    29221 atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt ggcgattctg
    29281 ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc aactcgcgtg
    29341 ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg cgggcaggcg
    29401 caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt ttgcccgccg
    29461 ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc acataccctg
    29521 gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag cgtttcgcat
    29581 ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg ggcgttacta
    29641 atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca gatcaatctc
    29701 attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg tagcgagtta
    29761 ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg cctggctggc
    29821 agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt ggctacgggt
    29881 gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta atttttatct
    29941 cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat tcctgcatct
    30001 ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca taacataaac
    30061 aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac agttttattc
    30121 aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt gccgatcatt
    30181 taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc ccaagctatg
    30241 tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc gttaaagagg
    30301 cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg gcacacgaag
    30361 cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg gcgctacata
    30421 actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg gcggggtttg
    30481 tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc cacggtcgcg
    30541 acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat aatttcccgc
    30601 tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc gcctggcata
    30661 aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg acgaccaatc
    30721 cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa cataaaggct
    30781 acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt aaaacgacgc
    30841 atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc actatcatca
    30901 tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc tttgccgagt
    30961 gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc gagtgggaag
    31021 tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga agctggcagg
    31081 ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag gctttctgtc
    31141 agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca agggtaacat
    31201 aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga aatacttgtg
    31261 ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg cctgataaga
    31321 cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct tatccgtcct
    31381 acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt ggcggaagtg
    31441 atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc ttccagggca
    31501 tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt ctgtgcctgg
    31561 gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag agccatagca
    31621 gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt tgtataagtg
    31681 acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa atcgaagaga
    31741 attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa ccatttattt
    31801 gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta aaggcacgga
    31861 atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag ttaacgtaac
    31921 tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg taaatctttc
    31981 tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg ggcttgcggg
    32041 atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat ttcaccccga
    32101 gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc gtcatcgtta
    32161 gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta agtgtagcta
    32221 atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc gcagcagatt
    32281 atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc ttttcagcga
    32341 ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc ggatcatcga
    32401 ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca cgcaatgcag
    32461 gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat gttgtccaaa
    32521 ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta aaaacagcgt
    32581 ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg cgccatccac
    32641 atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg aaagggtacg
    32701 cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca cctcattttg
    32761 cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa cttgtgcacc
    32821 ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt gttcgcgaaa
    32881 atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac aagttctcca
    32941 aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt gagcaagaag
    33001 gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc gacgtaatcg
    33061 gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat agcgattaac ccgatacccc
    33121 agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac cgcgccgtct
    33181 gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat atcatcggtt
    33241 gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag cgatgaggaa
    33301 ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt atagccgcca
    33361 atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc ttcgacgcgt
    33421 ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt ttcgatatca
    33481 gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca tttggtgcca
    33541 atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact gttaccactg
    33601 ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac ggcatagtta
    33661 atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca gttcaccaga
    33721 ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac accagctggg
    33781 caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata ttcagtatga
    33841 taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg cagcgtatcg
    33901 aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt gcgcgtcagg
    33961 ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa cgtcaccgga
    34021 ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact gacatcatga
    34081 ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag aattttatta
    34141 ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc gctggtgggc
    34201 tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg ctttacgcgc
    34261 gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc aaaacgcatg
    34321 gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa gttatgagta
    34381 tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga atagcccggc
    34441 aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg cagataatta
    34501 agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac gccgtaacgc
    34561 tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac attcaggcgc
    34621 accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg gaaccaggcg
    34681 ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt gctgatcata
    34741 ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc tttaatcact
    34801 tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg aataccatca
    34861 atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc agtggtggag
    34921 ttaccgttct cacccgcaaa aaacgcaccc acgccgg
//
LOCUS       DAJTNP010000034        34896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTNP010000034 DAJTNP010000000
VERSION     DAJTNP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            851..3136
                     /gene="nrdA"
                     /locus_tag="OO160_001823"
     CDS             851..3136
                     /gene="nrdA"
                     /locus_tag="OO160_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5523972.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3370..4500
                     /gene="nrdB"
                     /locus_tag="OO160_001824"
     CDS             3370..4500
                     /gene="nrdB"
                     /locus_tag="OO160_001824"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5523973.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4500..4754
                     /gene="yfaE"
                     /locus_tag="OO160_001825"
     CDS             4500..4754
                     /gene="yfaE"
                     /locus_tag="OO160_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5523974.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4808..5458)
                     /gene="inaA"
                     /locus_tag="OO160_001826"
     CDS             complement(4808..5458)
                     /gene="inaA"
                     /locus_tag="OO160_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5523975.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5637..5879
                     /locus_tag="OO160_001827"
                     /pseudo
     CDS             5637..5879
                     /locus_tag="OO160_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5921..6997)
                     /gene="glpQ"
                     /locus_tag="OO160_001828"
     CDS             complement(5921..6997)
                     /gene="glpQ"
                     /locus_tag="OO160_001828"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5523976.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7002..8360)
                     /gene="glpT"
                     /locus_tag="OO160_001829"
     CDS             complement(7002..8360)
                     /gene="glpT"
                     /locus_tag="OO160_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5523977.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8633..10261
                     /gene="glpA"
                     /locus_tag="OO160_001830"
     CDS             8633..10261
                     /gene="glpA"
                     /locus_tag="OO160_001830"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5523978.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10251..11510
                     /gene="glpB"
                     /locus_tag="OO160_001831"
     CDS             10251..11510
                     /gene="glpB"
                     /locus_tag="OO160_001831"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5523979.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11507..12697
                     /gene="glpC"
                     /locus_tag="OO160_001832"
     CDS             11507..12697
                     /gene="glpC"
                     /locus_tag="OO160_001832"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5523980.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12890..13840
                     /locus_tag="OO160_001833"
     CDS             12890..13840
                     /locus_tag="OO160_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5523981.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13881..14351)
                     /locus_tag="OO160_001834"
     CDS             complement(13881..14351)
                     /locus_tag="OO160_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5523982.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14406..15608)
                     /locus_tag="OO160_001835"
     CDS             complement(14406..15608)
                     /locus_tag="OO160_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5523983.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15695..16237)
                     /locus_tag="OO160_001836"
     CDS             complement(15695..16237)
                     /locus_tag="OO160_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5523984.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16516..16941
                     /gene="nudI"
                     /locus_tag="OO160_001837"
     CDS             16516..16941
                     /gene="nudI"
                     /locus_tag="OO160_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5523985.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16980..17582)
                     /locus_tag="OO160_001838"
     CDS             complement(16980..17582)
                     /locus_tag="OO160_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5523986.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17890..19029
                     /gene="arnB"
                     /locus_tag="OO160_001839"
     CDS             17890..19029
                     /gene="arnB"
                     /locus_tag="OO160_001839"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5523987.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19033..20001
                     /gene="arnC"
                     /locus_tag="OO160_001840"
     CDS             19033..20001
                     /gene="arnC"
                     /locus_tag="OO160_001840"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5523988.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20001..21982
                     /gene="arnA"
                     /locus_tag="OO160_001841"
                     /pseudo
     CDS             20001..21982
                     /gene="arnA"
                     /locus_tag="OO160_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21979..22869
                     /locus_tag="OO160_001842"
     CDS             21979..22869
                     /locus_tag="OO160_001842"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5523989.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22869..24521
                     /gene="arnT"
                     /locus_tag="OO160_001843"
     CDS             22869..24521
                     /gene="arnT"
                     /locus_tag="OO160_001843"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5523990.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24518..24853
                     /gene="arnE"
                     /locus_tag="OO160_001844"
     CDS             24518..24853
                     /gene="arnE"
                     /locus_tag="OO160_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5523991.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24853..25239
                     /gene="arnF"
                     /locus_tag="OO160_001845"
     CDS             24853..25239
                     /gene="arnF"
                     /locus_tag="OO160_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5523992.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25233..25505)
                     /gene="pmrD"
                     /locus_tag="OO160_001846"
     CDS             complement(25233..25505)
                     /gene="pmrD"
                     /locus_tag="OO160_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5523993.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25610..26965)
                     /gene="menE"
                     /locus_tag="OO160_001847"
     CDS             complement(25610..26965)
                     /gene="menE"
                     /locus_tag="OO160_001847"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5523994.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26962..27924)
                     /gene="menC"
                     /locus_tag="OO160_001848"
     CDS             complement(26962..27924)
                     /gene="menC"
                     /locus_tag="OO160_001848"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5523995.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27924..28781)
                     /gene="menB"
                     /locus_tag="OO160_001849"
     CDS             complement(27924..28781)
                     /gene="menB"
                     /locus_tag="OO160_001849"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5523996.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28796..29554)
                     /gene="menH"
                     /locus_tag="OO160_001850"
     CDS             complement(28796..29554)
                     /gene="menH"
                     /locus_tag="OO160_001850"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5523997.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29551..31221)
                     /gene="menD"
                     /locus_tag="OO160_001851"
     CDS             complement(29551..31221)
                     /gene="menD"
                     /locus_tag="OO160_001851"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5523998.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31310..32605)
                     /gene="menF"
                     /locus_tag="OO160_001852"
     CDS             complement(31310..32605)
                     /gene="menF"
                     /locus_tag="OO160_001852"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5523999.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32684..32989)
                     /gene="elaB"
                     /locus_tag="OO160_001853"
     CDS             complement(32684..32989)
                     /gene="elaB"
                     /locus_tag="OO160_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5524000.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33044..33505)
                     /locus_tag="OO160_001854"
     CDS             complement(33044..33505)
                     /locus_tag="OO160_001854"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5524001.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33570..34487
                     /gene="rbn"
                     /locus_tag="OO160_001855"
     CDS             33570..34487
                     /gene="rbn"
                     /locus_tag="OO160_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5524002.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34678..34866
                     /locus_tag="OO160_001856"
                     /pseudo
     CDS             34678..34866
                     /locus_tag="OO160_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8534 a         9338 c         8646 g         8378 t
ORIGIN      
        1 cctgaaaggc tttgcctgct ggcgtgacaa ctcgctttga tatcatatcc ctggcataaa
       61 tcaatggccg ccgcgacacc gttagcagag aaaagagatg caatcattga cgggagtaga
      121 gataaatact ccttgcgtag aaagataatc cgcatattaa tctgaccatc attttatata
      181 agacggcata tttaaacgca ttatacaata tgggttatct ttcttttgta aattgtccaa
      241 cccgatgcaa tggctatttt gttattattt gatgaataat atcagtgcgt cataattcaa
      301 gttaataacc ttaagggata ccagttatat ttaaactaaa ttaaagtaat gaataatttt
      361 cttataatat aaggtaaatt aacaaaatgg cttcgcattt aacaataacc gaatagaaaa
      421 caaccatttc gacatcaaca atcgcttaca ttcgcttata tattgaccac aactgataca
      481 tcagattatg tgatgactcg tgcttagatc aatttttgca atcattagca aaaagattaa
      541 taaaccatct atatcaattt atccaaccta ttctgccgtt caagaaatcg ccgaacagtt
      601 atttttaaca aatttttctc ttcccattga ctttcccgga caccttgtct gacctaaggt
      661 gcgcgaaagc cactttttcc ttcctgagtt atccacaaag ttatgcactt gcaagagggt
      721 cattttcaca ctatcttgca gtgaatccca aacatacccc ctatatatag tgttctaagc
      781 agcttccctt actacaggta gtctgcatga aactattgcg gaaagaattc caaaaacagg
      841 tacgacatac atgaatcaga atctgctggt gacaaagcgc gacggtagca cagagcgcat
      901 caatctcgac aaaatccatc gcgttctgga ttgggcggca gaaggactgc ataacgtttc
      961 gatttcccag gtcgagctgc gctcccacat tcagttttat gacggtatca agacctctga
     1021 catccacgaa actattatca aggctgccgc agacctgatc tcccgtgatg cgccggatta
     1081 tcagtatctc gccgcgcgcc tggcgatctt ccacctgcgt aaaaaagcct acggccagtt
     1141 tgagccgcct gcgctgtacg accacgtggt gaaaatggtc gagatgggca aatacgataa
     1201 tcatctgctg gaagactaca cggaagaaga gttcaagcag atggacacgt ttatcgatca
     1261 cgaccgtgat atgaccttct cttatgctgc cgttaagcag ctggaaggca aatatctagt
     1321 acagaaccgc gtgaccggcg aaatctacga aagcgcccag ttcctttata ttcttgttgc
     1381 cgcgtgcctg ttctcgaact acccgcgtga aacgcgcctg caatacgtga agcgttttta
     1441 tgacgcggtt tccacattta aaatttcgct gccgacgcca atcatgtccg gcgtgcgtac
     1501 cccgactcgt cagttcagct cctgcgtact gatcgagtgc ggtgacagcc tggattccat
     1561 caacgccacc tccagcgcga ttgtgaaata tgtttcccag cgtgccggga tcggcatcaa
     1621 cgccggacgt attcgtgcgc tgggcagccc gattcgcggt ggcgaagcgt tccataccgg
     1681 ctgtatcccg ttctacaaac atttccagac agcggtgaaa tcctgctctc agggcggtgt
     1741 gcgcggcggt gcggcaacgc tgttctaccc gatgtggcat ctggaagtgg aaagcctgct
     1801 ggtgttgaaa aacaaccgtg gggttgaagg caaccgcgtg cgtcatatgg actacggggt
     1861 acaaatcaac aaactgatgt atacccgcct gctgaaaggt gaagatatca ccctgttcag
     1921 cccgtccgac gtaccggggc tgtacgacgc gttcttcgcc gatcaggaag agtttgaacg
     1981 cctgtatacc aaatatgaga aagacgacag catccgcaag cagcgtgtga aagccgttga
     2041 gctgttctcg ctgatgatgc aggaacgtgc gtctaccggt cgtatctata ttcagaacgt
     2101 tgaccactgt aatacccata gtccgtttga tccgaccatc gcgccagttc gccagtctaa
     2161 cctgtgccta gaaattgccc tgccgaccaa accgctgaac gacgtcaacg acgagaacgg
     2221 tgaaatcgcg ctgtgtacgc tgtctgcttt caacctgggc gcaattaata gcctggatga
     2281 actggaagag ctggcgattc tggcggttcg tgcacttgac gcgctactgg attatcagga
     2341 ttacccgatc ccggccgcca aacgtggagc gatggggcgt cgtacgctgg gtattggtgt
     2401 gattaacttc gcttactatc tggcgaagca cggtaaacgc tactccgacg gcagcgccaa
     2461 caacctgacg cataaaacct tcgaagcgat tcagtattac ctgctgaaag cgtctaatga
     2521 gctggctaaa gagcaaggcg cgtgcccgtg gtttaacgaa accacttacg cgaaagggat
     2581 cctgccgatc gatacctata agaaagatct ggatgccatc gctaatgagc cgctgcatta
     2641 cgactgggaa gcattgcgtg aatcaatcaa aacgcacggc ctgcgtaact ccacgctttc
     2701 tgccctgatg ccgtccgaga cctcttcgca gatctctaac gccactaacg gtattgaacc
     2761 gccgcgcggt tacgtcagca tcaaagcgtc gaaagacggt attttgcgcc aggtggtgcc
     2821 ggactacgag cacctgcacg acgcctatga gctgctgtgg gaaatgccgg gtaacgatgg
     2881 ctacctgcaa ctggtaggta tcatgcagaa atttatcgat cagtcgatct ctgctaacac
     2941 caactacgat ccgtcacgtt tcccgtcagg aaaagtgccg atgcagcagt tgctgaaaga
     3001 cctgctcacc gcctacaaat tcggggtcaa aacgctgtat tatcagaaca cccgtgacgg
     3061 cgctgaagac gcgcaggacg atctggtacc gtcaatccag gacgatggct gcgaaagcgg
     3121 cgcatgtaag atctgatatt gagatgccgg atgcggcgta aacgccttat ccggcctacg
     3181 gctcggtttg taggcctgat aagacgcgcc agcgtcgcat caggctccgg gtgccggatg
     3241 cagcgtgaac gccttatccg gcctacggct cggatttgta ggcctgataa gacgcgccag
     3301 cgtcgcatca ggcacaggat gcggcgtaaa atgccttatc cggcattaaa ctcccaacag
     3361 gacacactca tggcatatac caccttttca cagacgaaaa atgatcagct caaagaaccg
     3421 atgttctttg gtcagccggt caacgtggct cgctacgatc agcaaaaata tgacatcttc
     3481 gaaaagctga tcgaaaagca gctctctttc ttctggcgtc cggaagaagt tgacgtctcc
     3541 cgcgaccgta tagattacca ggcgctgccg gagcacgaaa aacacatctt tatcagcaac
     3601 ctgaaatatc agacgctgct ggattccatt cagggtcgta gcccgaacgt ggcgctattg
     3661 ccgcttattt ctattccgga actggaaacc tgggtcgaaa cctgggcgtt ctcagaaacg
     3721 attcattccc gttcctatac tcatatcatt cgtaatatcg ttaacgatcc gtctgttgtg
     3781 tttgacgata tcgtcaccaa cgagcagatc cagaaacgtg cggaagggat ctccagctat
     3841 tacgatgaac tgatcgaaat gaccagctac tggcaccttc tgggcgaagg cacccacacc
     3901 gttaacggta aaaccgtaac cgttagcctg cgcgagctga agaaaaaact gtatctctgc
     3961 ctgatgagcg ttaacgcgct ggaagcgatc cgcttctacg tcagctttgc ttgttccttt
     4021 gcctttgcag aacgcgaact gatggaaggt aacgccaaga ttattcgcct gattgcccgc
     4081 gatgaagccc tgcacctgac cggtactcag catatgctga atctgctgcg cagcggcgcg
     4141 gatgatccgg agatggcgga aattgccgaa gaatgtaagc aggagtgcta tgacctgttt
     4201 gtccaggcgg cacagcaaga gaaagactgg gcggattatc tgttccgtga cggttcgatg
     4261 attggtctga ataaagatat tctctgccag tacgttgaat acatcaccaa tatccgcatg
     4321 caggcagtcg gtttggatct gccgttccag acgcgctcca acccgatccc gtggatcaac
     4381 acctggctgg tgtctgataa cgtgcaggtt gctccgcagg aagtggaagt cagttcttat
     4441 ctggtcgggc agattgacgc ggaagtggac accgacgatt tgagtaactt ccagctctga
     4501 tggcccgcgt taccctgcgc atcactggca cacaactgct gtgccaggat gaacaccctt
     4561 cccttctggc ggcgctggaa tcccacaatg tggcggttga gtaccagtgc cgcgaaggtt
     4621 actgcggctc ctgtcgcacc cgactggtcg caggtcaggt ggactggatt gccgaaccgt
     4681 tagcctttat tcagccgggg gaaattttgc cctgttgttg ccgggcaaaa ggcgatattg
     4741 aaatcgagtt gtgatcgggt actagcgcca gatacgacgc ttacgcgtat tatctggcct
     4801 acaacgatta catggcgtag taatacgctt tcacctgctc ccagtccgct ttagggatcg
     4861 gctccagata tttttccagc tggcggaagt catgattaat cgctttatcg cgacgcaagc
     4921 ggcgacgact tttctccaga tcaagaaaac cagcttctgc cttaccttct gttttcacat
     4981 agatatggcg aacatagcaa cagccatgct gacgattaac gctatgcatt ttcttaaacg
     5041 ccagtgccac tgctttcaac atggcttgcc gtacttcgtc agaataaggc gatactgcat
     5101 gccgggcata ccagtcagca atgctgatga accccgccat atcttcagtc accagcaacg
     5161 cgcgccattc accctcaatt ttcaccgctt caccaaaaac gatcttcggc acgatgacac
     5221 cggcccgttc cagttcttta attaccgcaa cctcacggac aatcgttggt cggccgaacg
     5281 gataacgtac tgaatgaaac agatgatgcg tcatgcgctt tacatacagc tttttgccgt
     5341 tgcgctcgac gcattgcacc ccgctcatac cattacggcg atagttaggc tcttcaaccc
     5401 agtcgccctc tgttgcccac cagtgattaa attcgtcgta ctttgctgaa actgccatac
     5461 cctatcgcct gtcattttta ttaacgacaa tgactatagg tggttacctg aggaaaatct
     5521 taatgaaacg tgtcgtatta atgaatttct tttgccgcta caggtgtgac accgcttttg
     5581 cccttcattt gcagcgcata gattccacca gccactacca ctaatagtgc gcccagcaga
     5641 taaaatgtga atttaatcag tcggatacag cgatgaattt tattcgacag ggattaggca
     5701 tcgccttaca accagagtta acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc
     5761 acgaaccaac cttctatcga cagatttcgt tgctgactaa agaaaagccg gtagaaggca
     5821 gtccactgtt tttactacaa atgtgcatgg aacaattagt ggcgattgga aaaatttgat
     5881 atgagacagg atggcgcatg aacgccatcc tgcagtaaca ttactcttta ttaagaaatt
     5941 ttactgcctt atcagggaaa tcagtaaaca gcccatttac acccgctttg ttatacagag
     6001 catcatataa ctgattcaca tcaggagtgt attcaggcag tttatctgac cgcacggtat
     6061 aaggatgcac taccagttta ttctgctggg catcttgcac catgccagtg agtttgatat
     6121 tacccggctg cgatgtctcc tcaatcaaca tatggtaatc cggaccaata ccatctgcat
     6181 attccgccac ctgtttcatg gctcccggct taaacatcca gtcgtagttg taattaaccc
     6241 agcttccatc cggctgtttc tgctgcgttt cattccagtc ggtataggca atcagctgta
     6301 ccagattgag ctccatgccc attttgggtt ccagctcatt cttaatacgc ttcagctcat
     6361 cagcatcaaa acattgcaaa taaactttat cgtctttacc ggtgtaacca tatttcttca
     6421 gcacttccag cgtttttgcc gcaatatcct tcccttcctg atgatggaac cacggcgctt
     6481 tgatttctgg atagataccg atatttttcc cggtagagtg atttaacccc tgaacaaatt
     6541 caatctcttc ttcaaaggtg tgcacccgga agtcggactt acccattggg aaacgccccg
     6601 gataagtctg tactttttta ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact
     6661 taatttcatc cagcgtgaaa tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg
     6721 ggaaacgatc ggcaacatca gtaacacgat cgagataatg gtcatgcaga acaaccagat
     6781 ggtcgtcttt ggtcatcacc aaatcctgtt ccagataatc cgctccctgc gcatacgcca
     6841 tcgcttttgc tggcagcgta tgctccggca aatatccact ggcaccgcga tgggcgatga
     6901 ctattttttc gttgctgtcc gccgccattg cactgcttcc catgactata gtgctcatca
     6961 tgatcgccat gctaaggttt ttcagcttca atttcatgcc attagcctcc gttgcgtttt
     7021 tgcagtaatt gttcatggcg acgtttttcg ccaatcatca caacaatcaa caagataacc
     7081 gccagaatgc tgccgccaat cattaccata aagccgccat cccagccgaa gaagtccacg
     7141 gtgtagccaa caatcgcgct cgccgccacc gaaccgccca ggtaaccaaa cagcccggta
     7201 aagcccgctg ccgtacctgc cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc
     7261 agcatcacag gaccgtagat caggaagccg ataacaatca tacaaatcat atcgacggtt
     7321 gggttacctg ccgggttcat ccagtaaacg atagtcgcga tggtcaccag tgtcataaag
     7381 aagacgccgg ttgccccacg gttaccacgg aagactttgt ccgacatcca gccgcacagc
     7441 agggtgcccg gaatacccgc atactcatag aagaagtagg cccaggagga tttatccagc
     7501 gcgaaatgct taacctcttt cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc
     7561 agataaacga acacgttggc gatggcgatg taccacagca gtttgttcgg cagcacgtac
     7621 tgcatgaaga tttgcttcgc ggtcagctcc tgttccgctt tttcgttata gtcgtccgga
     7681 taatcatttt tgtactcttc gatcggcggc aagccacagg attgcggggt atcgcgcatc
     7741 atcgcaaagg cgaataatgc caccagaatg gcgcagaaag caggcatata gagcgccgca
     7801 tgccagtcat tgaaccaggc catccccagc aggaacagca gcggcggaat accaccaccg
     7861 acgttgtgcg cacagttcca cactgacaca atgccgccac gttctttctg cgaccaccag
     7921 tgcaccatag tacgaccaca cggcggccac cccatcccct ggaaccaacc gcagaggaac
     7981 aacagtacaa acatcaccgc aatgctcgac gtcgcccatg gcacaaagcc cataaacaac
     8041 atcactgccg ccgccagaat caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc
     8101 gataccgaac ccatgatgaa tttcgaaaat ccataagcaa tcgagatccc cgaaagggca
     8161 aaacctaaat caccgcgtga gaatccctgc tcaaccagat aaggcatagc aagcgcaaag
     8221 ttcttacgaa ccaaatagta agccgcatag ccaaagaata tccccaggaa aatttgccag
     8281 cgcaatcgac gataagtcgg atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct
     8341 ggtttaaaaa tactcaacat tgatagcctc cgtggcccgt ggtcttattt atgattaaca
     8401 gcctgattca gtgagagaac ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc
     8461 ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa tgttcaaaat
     8521 gacgcatgaa atcacgtttt actttcgaat catgagcgaa tatgcgcgaa atcaaacaat
     8581 tcatgttttt actatggcta aatggtaaaa aacgaacttc agagggataa caatgaaaac
     8641 tcgcgactcg caatcaagtg acgtgattat cattggcggc ggcgcaacgg gagccgggat
     8701 tgcccgcgac tgtgccctgc gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc
     8761 aaccggtgcc accgggcgta accacggcct gctgcacagc ggtgcgcgct atgcagtaac
     8821 cgatgcggaa tcggcccgcg aatgcattag tgaaaaccag atcctgaaac gcattgcacg
     8881 tcactgcgtt gaaccaacca acggcctgtt tatcaccctg ccggaagatg acctctcatt
     8941 ccaggccact tttattcgcg cctgcgaaga agcagggatc agcgcagaag ctatagaccc
     9001 gcagcaagcg cgcattatcg aacctgccgt taacccggca ctgattggcg cggtgaaagt
     9061 tccggatggc accgttgatc catttcgtct gaccgcagca aacatgctgg atgccaaaga
     9121 acacggtgcc gttatcctta ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac
     9181 ggtgtgcggt gttcgtgtac gtaaccatct caccggcgaa actcaggccc ttcatgcacc
     9241 tgtcgtggtt aatgccgctg ggatctgggg gcaacacatt gccgaatatg ccgatctgcg
     9301 cattcgcatg ttcccggcga aaggatcgct gctgatcatg gatcaccgca ttaaccagca
     9361 tgtgatcaac cgctgccgta aaccttccga cgccgatatt ctggtgcctg gcgataccat
     9421 ttcgctgatt ggtaccacct ctttacgtat tgattacaac gagattgacg ataatcgagt
     9481 gacggcagaa gaggttgata ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc
     9541 gaaaacgcgc attttgcggg cctattctgg cgtgcgcccg ctggttgcca gcgatgacga
     9601 cccgagcgga cgtaacgtca gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg
     9661 tctggacgga tttatcacca tcaccggtgg caaactgatg acctatcggc tgatggctga
     9721 atgggctacc gacgcggtat gccgcaaact gggcaacacg cgcccctgta cgactgccga
     9781 tctggcactg cctggttcac aagaacccgc tgaagttacc ttgcgtaaag tcatctccct
     9841 gcctgccccg ctgcgcggtt ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct
     9901 gagcgaaggc cgtctacacc gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga
     9961 agtgcagtac gcggtagaaa atttaaacgt taatagcctg ctggatttac gccgtcgtac
    10021 ccgtgtgggg atgggcacct gccagggcga actctgcgcc tgccgcgctg ccggactgct
    10081 gcaacggttt aacgtcacga cgtccgcgca atctatcgag caactttcca ccttccttaa
    10141 cgaacgctgg aaaggcgtgc aacccatcgc ctggggagac gcactgcgcg aaagcgaatt
    10201 tacccgctgg gtttatcagg gactgtgtgg tctggagaag gagcagaaag atgcgctttg
    10261 atactgtcat tatgggcggc ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac
    10321 acggcctgcg ctgtgccatt gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt
    10381 cgctggattt gctgagccat ctgccagatg gtcaacccgt ggcagacatt cacagtggac
    10441 tggaatcttt gcgtcagcag gcacctgcac atccttactc tcttctcggg ccacaacgcg
    10501 tgctcgatct cgcttgccag gcgcaggcat taatcgctga aagcggtgcg caattgcagg
    10561 gcagcgtaga actggctcac cagcggatta cgccgctcgg cactttgcgt gcaacctggt
    10621 taagttcgcc agaagttccc gtctggccgc tacccgcgaa gaagatatgt gtagtgggga
    10681 taagcgggct gatggatttc caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc
    10741 tttcggttga aaccgcagaa atagagctgc cggaactgga tgtcctgcgc aataacgcca
    10801 ccgaatttcg cgcggtgaat atcgcccgtt tccttgataa tgaagaaaac tggccgctgt
    10861 tacttgatgc gcttattcct gtcgccaata cctgcgaaat gatcctgatg cccgcctgct
    10921 tcggcctggc cgatgacaaa ctgtggcgtt ggttgaatga aaaactacct tgttcactga
    10981 tgcttttgcc aacgctgccg ccttccgtgc tgggcattcg tctgcaaaac cagttacagc
    11041 gccagtttgt gcgccaaggt ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct
    11101 gtaaaaatgg cgtagtgaat gaaatctgga cccgcaatca cgccgatatt ccgctacgtc
    11161 cacgtttcgc ggttctcgcc agcggcagtt tctttagtgg cggactagta gcggaacgta
    11221 acggcattcg cgagccgatt ctcggccttg atgtgctaca aaccgccacg cggggtgaat
    11281 ggtataaggg cgattttttt gcgccgcaac cgtggcagca gttcggtgta accactgatg
    11341 agacgctacg cccgtcgcag gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg
    11401 tgctgggcgg atttgatccc atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg
    11461 ctttacatgc cgctcaacag attgcccaac gcgcaggagg ccaacaatga acgacaccag
    11521 cttcgaaaac tgcattaagt gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa
    11581 tcccggttat ccagggccaa aacaagccgg gccggatggc gagcgtctgc gtttgaaaga
    11641 tggcgcactg tatgacgagg cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc
    11701 ctgcccgtcc gatgtgaaga ttggcgatat tatccagcgc gcgcgggcga aatatgacac
    11761 cacgcgcccg tcgctgcgta attttgtgtt gagtcatacc gacctgatgg gtagcgtttc
    11821 cacgccgttc gcaccaatcg tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct
    11881 tgatgcggcg ttaaaaatcg atcatcgtcg cacgctaccg aaatactcct tcggcacgtt
    11941 ccgtcgctgg tatcgcagca tagcggctca gcaagcacaa tataaagacc aggtcgcttt
    12001 ctttcacggc tgcttcgtta actacaacca tccgcagtta ggtaaagatt taattaaagt
    12061 gctcaacgca atgggtaccg gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc
    12121 gctaatcgcc aacggcttta ccgataaagc acgcaaacag gcaattacga atgtagagtc
    12181 gatccgcgaa gctgtgggag taaaaggcat tccggtgatt gccacctcct caacctgtac
    12241 atttgccctg cgcgacgaat acccggaagt gctgaatgtc gacaacaaag gcttgcgcga
    12301 tcatatcgaa ctggcaaccc gctggctgtg gcgcaagctg gacgaaggca aaacgttacc
    12361 gctgaaaccg ctgccgctga aagtggttta tcacactccg tgccatatgg aaaaaatggg
    12421 ctggacgctc tacaccctgg agctgttgcg taacatcccg gggcttgagt taacggtgct
    12481 ggattcccag tgctgcggta ttgcgggtac ttacggtttc aaaaaagaga actaccccac
    12541 ctcacaagcc atcggcgcac cactgttccg ccagatagaa gaaagcggcg cagatctggt
    12601 ggtcaccgac tgcgaaacct gtaaatggca gattgagatg tccacaagtc ttcgctgcga
    12661 acatccgatt acgctactgg cccaggcgct ggcttaaact cccttctgat gcccggtaag
    12721 catgtggtta ccgggcattt ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg
    12781 cagtgaaaat tcctctgaaa acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct
    12841 gggataaccg taatgtcgcg tcatcataaa tatcaggtga cggacaacca tgaccgaatc
    12901 aacaacctcc tccccgcatg atgcggtatt taaaaccttt atattcacac ccgaaaccgc
    12961 acgggatttt ctcgaaatac atttaccaga accactgcgc aagctttgca acctgcaaac
    13021 cttacgcctg gaacccacta gttttattga aaaaagttta cgcgcttact actcggatgt
    13081 tttgtggtcc gtggaaacca gcgacggtga cggctatatc tactgcgtga ttgaacatca
    13141 aagctctgca gaaaagaata tggcttttcg gccaatgcgc tatgccactg ccgccatgca
    13201 gagtcaccta gataaaggct atgacagagt tccgctggtg gtgccattgc tgttttatca
    13261 tggcgaaaca tcgccctacc cgtactcact taactggctg gatgagtttg acgatccgca
    13321 acttgcccgg cagttgtaca ccgaagcttt tccgttggtg gatatcacca tcgtacctga
    13381 cgatgagatc atgcaacatc ggcgtatagc tctgctggaa ctgattcaaa agcatattcg
    13441 cgaccacgat ttaatcggca tggtcgacag gatcaccacg cttttggtta gaggcttcac
    13501 taatgacagc cagctacaaa cactgtttaa ttatctgctg caatgcggcg atacctcccg
    13561 tttcacccgt tttattcagg agattgccga acgttcacca ctacaaaagg agagattaat
    13621 gactattgct gaacggctac ggcaggaagg acatcaaatt ggctggcaag aaggtaaatt
    13681 agtaggtttg cagcaaggta aattagaagg tttgcaggaa ggtatgcatg aacaagccat
    13741 taaaattgct ttgcgcatgc tggaacaggg cattgatcgt gaccaagtgc tcgcggccac
    13801 ccagctaagc gaagccgatc tggcagcgaa taaccactaa ttaacacagg cccacagccg
    13861 atccccatgg gcctttgata tcaataacta ccttttatgc gtggcccatt tttgcctgat
    13921 ttaaacatcg ccagtcgttg atccagggca tcgctgtaga gcatcgtgtc aacgccaaca
    13981 gcgacaaagt tcgctcccca cgccaggcat tgctgcgcca tatcaggagc cacagccaga
    14041 aaaccagccg ctttacccgc agcacggatc cgccgaatgc tggtttcaat aattcgctgc
    14101 acttccgggt gcccggcgtt atccgggtag cccaacgacg cagaaagatc cgctggtcca
    14161 ataaacacgc catcaatccc ttcgacgtcg aggatctcgt ccaggttatc cagtgccgtt
    14221 ttactttcca cctgcactaa cagacaaagc gaatcgttga cttgcgccat gtaattctca
    14281 atgcgtcccc agcgcgcagc ccgtgccaca ctggccccaa caccacgctc accgtaggga
    14341 gggttggaca aattttgccg gagggggaat gtgccggtgc agtaaaccgg cacagagtgg
    14401 cgaaatcaga cggataaagt catggactca acaacctcaa tccagccatg ctcactagcg
    14461 atgtcctggc cgtttaacca acggcgtagc atattcagtg ccatcattgc gcacacttcc
    14521 tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc gaaagtgccg
    14581 tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac agccagtgcc
    14641 agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg cgccagggtt
    14701 tcttcctgtg aaggaatcac ttcacacgcc agcaatggag cacctgcgcg agaaagttgc
    14761 aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg gcggttttgc
    14821 aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac gctctgtccg
    14881 gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct tgccggtccg
    14941 gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac gcccggcggc
    15001 agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa ggtagttaaa
    15061 cgcagacaaa ccggcggctg tggtaaagaa aaacgctcgc gcaggcgcgg caggatttcg
    15121 tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat caggcaacga
    15181 ttaagctgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa ttcagcactg
    15241 gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg ttcgtggaaa
    15301 tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc ttttgctgtc
    15361 gcagcggcga gtgcgcttaa atcatcgctg gtcggtccca gcccgccgtt aacgatcagc
    15421 acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag gttatccccc
    15481 accgtattgc ggcgagataa tggcagcccc tgatgaaaga aaaaatcggc cagccaggca
    15541 gcgttagtgt caacgatttg cccgtataac acttcatccc cggtggatag catttccact
    15601 tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag ggagataagg
    15661 cgtagtaaga aactgaatgc cgcgccggaa gggatcagaa actggcgtta accccaacat
    15721 acaggccgtc agccacagcg ttgtcgcggt taccgtcttt acccgacaga ttcaggtagc
    15781 gataacccgc ctcaatactg actggacgca taatggtgta acgcgcgcca gcattcgctt
    15841 cctcataact ttgaataccg ctggagagcg aatccggaga gtagtaatac tcgccaaaca
    15901 aacggaagct gttgccaatt ttccactgca aaccacctcc taccgctgcg gcataacctt
    15961 catcgccgta attcgggttg gtgtacacgc cttttccgcc aacggtcgcc attaacggcc
    16021 cgagaggcag attcaacccc agccccacgc ccgcgacgtc accgtcgtcg tcgttatgtg
    16081 tccagttacc gcttaaagcc aggcccgtcg attcagtccc aaaaccgaca ccaatattgg
    16141 tgtattcttt acccgcctgg ccgctgatgc tcattgcatt gaccgatgca gaaaccaaca
    16201 gcattccggc cagacctgct agcgcaattt ttttcattat cccctttccc acaagcaatt
    16261 aatcaaaatt cccaaagctg cgggattgta ctgcctggca cagaggattc aatgagcgaa
    16321 aaaagggatc tagtcactta ttgtaactaa atacttctta aataattaaa cgccgaaaaa
    16381 ataaacacac tttaccgata cacacaaaaa taataataag tgcagttatc attgcaaaag
    16441 agattactgt cactttcaca tatttctgag ttaccgtatt ctcatggttt ctttttattt
    16501 ataaggaata cccctgtgcg acaacggact attgtatgcc ctttgattca aaatgatggt
    16561 gcttatttgc tgtgcaaaat ggccgacgat cgcggcgttt tccccggtca atgggcgctt
    16621 tcgggtggcg gcgtggagtc tggcgaacga attgaagagg cactacgccg cgaaattcgc
    16681 gaagaactgg gagaacagct gcttttgaca gaaatcacgc cgtggacctt cagcgatgat
    16741 attcgcacca agacatatgc agatggtcgc aaggaagaga tttatatgat ttacctgatt
    16801 tttgactgcg tttctgccaa ccgagaagtg aaaataaacg aagagtttca ggactacgcg
    16861 tgggtaaaac ctgaagatct ggtgcattat gatttaaatg ttgccacccg aaaaacgtta
    16921 cgtttgaaag gtcttcttta acaatgatga cagccatcac tggctgtcag attgaaaatt
    16981 tagtggttaa cgaattcccc atccagataa actttgcctt tttccacatg catgactaaa
    17041 ccatccagat aatcaggttt aaatatcgca tcacgcttat ttttagcaat atatgtcagg
    17101 caatgattat gggtgaaaat aacgatattt ttatcaggcg ctttgttttg taagtcctta
    17161 attgcactat aaatctcatt accgcactga agaagtcgtt tatctaccgt cagcttttta
    17221 cccgcggaaa accaggtcgc cgactgaatg gtccggacgg tattactgga ataaagatcg
    17281 aaatcagaga tgtcagcgct aaaagcgttg cccagttcac gggcatcctg ggtaccttta
    17341 accgtaatac ctgttttatc tgacaagcat tgattggttg aacgatcgca acgttcagca
    17401 tggcgaaaca aaacgacaac cgggtgctgc tgtgccagtc tggccaatgt tttattgtcg
    17461 atacgtggca aaccattgct actccaggcg gcatgagtac ccagtccggc aattgcagcg
    17521 agcgccagta aaatgatgaa atattttttt gacttcaatg atgagcggca aaaagctaac
    17581 acgtaaactc cacctataga caagcgcaac cagacaatta ccgtgaaatt gagcgacatt
    17641 tctggcgata attagcagtt ggtgtaatat taaaaatcct acgatgtcgg caaaatgcct
    17701 caaatttttg ccaaatgcaa agcctaaata agaaaaaata taaaaatttc aatatttacg
    17761 tctaatatta gtttcttaag gttaagttaa tattctatcc ttaaaatttc gctccaaatg
    17821 gcaaaatata cacaacactc tttatagcaa atataagtgg acaggtattc aatggcggaa
    17881 ggaaaagcaa tgtcagaatt tttgcctttt tcgcgaccag caatgggcgt ggaggaactc
    17941 gctgcagtta aagaggttct cgaatccggt tggatcacaa ccggtccgaa gaatcaggcg
    18001 cttgagcaag ccttttgcca gttgacggga aatcagcatg ccatcgcggt cagttcagcc
    18061 accgccggaa tgcatatcac gctaatggcg ttggaaattg gcaagggcga tgaagtgatt
    18121 acgccttccc tgacctgggt ttcaaccctc aatatgattt ccttgctggg tgcaacgccg
    18181 gtaatggtgg atgtcgaccg cgatacgctg atggtcacgc ctgaagcgat cgaatcagcc
    18241 attacgccac gcactaaagc catcattccg gtgcattatg ccggtgcgcc agcagatatt
    18301 gacgccattc gcgccattgg cgaacgttac ggcatcgcag ttatcgaaga tgctgcccat
    18361 gccgtcggta cgtattacaa agggcgacat attggcgcaa aaggtaccgc tattttttca
    18421 tttcatgcca ttaaaaatat tacctgtgct gaaggtggcc tgattgtaac tgataatgaa
    18481 aaccttgccc gccagctacg gatgctgaaa tttcacggtc tgggtgtcga tgcctatgac
    18541 agacaaacct ggggccgtgc accgcaggct gaagtcttaa caccgggcta taagtacaat
    18601 ctgaccgata ttaacgccgc gattgccctg acacagttag tcaaattaga gcacctcaac
    18661 acccgtcggc gcgaaattgc ccagcaatat cagcaagcac tggcagctct cccctttcag
    18721 ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc atctgtttat tattcgtgtc
    18781 gatgaacaac gttgtggtat cagtcgcgat gcgttgatgg aagcgttaaa agaaagaggc
    18841 attggtaccg ggttacattt ccgcgccgct cacacacaaa aatattatcg cgagcgtttt
    18901 cccacgctgt cgttaccgaa taccgaatgg aatagcgaac gcatctgttc gttgccgctg
    18961 ttcccggata tgactaccgc cgatgccgac cgcgtcatca cagcccttca gcaactcgca
    19021 ggacaataag ccatgtttga aatccaccct gttaagaaag tctcggtggt tattcccgtt
    19081 tataacgagc aggaaagctt accggaatta atcaggcgca ccaccacagc ctgtgaatcg
    19141 ttggggaaag agtatgagat cctgctgatt gatgacggca gtagcgataa ttccgcgcat
    19201 atgctggtcg aagcctcaca agcggagaac agccatattg tgtctatttt gattaaccgc
    19261 aattacgggc aacattcagc gattatggcg ggattcagtc acgttactgg cgacttaatt
    19321 attacccttg atgccgatct ccagaatccg ccagaagaaa tcccccgcct ggtggcaaaa
    19381 gccgatgaag gttacgacgt ggtagggact gtacgccaga accgccagga cagctggttt
    19441 cgtaaaacag cttcgaagat gattaaccgg cttattcagc gcaccactgg caaagcgatg
    19501 ggtgactacg gttgtatgct gcgcgcctat cgccgtcata ttgtcgatgc gatgttgcac
    19561 tgccatgaac gcagcacctt tatcccgatt ctggcgaata tcttcgcccg ccgtgcaatt
    19621 gaaattccag tacatcatgc cgagcgtgag tttggtgaat ccaaatacag ttttatgcgc
    19681 ctgattaatt tgatgtacga cctggtgacc tgccttacca caacgccgct acgtatgctg
    19741 agtctgctcg gcagcattat tgcgattgga ggttttagca ttgcggtgct gctggtgatt
    19801 ttacgcctga ccttcggacc acaatgggcg gcagaaggcg tctttatgct atttgccgtg
    19861 ctgtttactt ttattggcgc tcagtttatc ggcatgggat tactcggtga atatatcggc
    19921 aggatctaca ccgatgtccg cgcccgcccc cgctattttg ttcagcaagt tatccgtcca
    19981 tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc ctaccacgat atgggatgcc
    20041 tcggtattga agctctgctg gctgccggtt acgaaattag cgccattttt acccataccg
    20101 ataatcccgg tgaaaaagcc ttttatggtt cggtggctca tctggcggcg gaaagagaca
    20161 ttccggttta tgcgccggat aacgttaatc atccgctgtg ggtggaacgc attgcccaac
    20221 tgtcaccaga ggtgattttc tctttttatt atcgccatct tatttgcgac gagatttttc
    20281 agctcgctcc cgcaggtgca tttaatctgc acggttcgct gttaccaaaa tatcgtggtc
    20341 gcgcgccgct gaactgggtg ctggtgaacg gtgaaacgga aacgggcgtt acattgcacc
    20401 gaatggtaaa cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc
    20461 agacgatatc gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga
    20521 acagacatta cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga
    20581 agccacctgt tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc
    20641 atccgtactg cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta
    20701 tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc
    20761 acagccgggg agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct
    20821 ggaaatcgtc accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca
    20881 gatgctgggc ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg
    20941 ccgtacccgg gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg
    21001 cctgctgcgc gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag
    21061 ccgttttctg aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga
    21121 atggattgag tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac
    21181 gccgattgaa tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct
    21241 gcgcattatc cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga
    21301 agtttatggg atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg
    21361 cccggtgaat aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat
    21421 ctgggcctat ggcgaaaaag agggtttaca gttcaccctc ttcctcccgt ttaactggat
    21481 gggaccacga ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca
    21541 actcattctc aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa
    21601 acgctgcttt actgatatcc gggacggtat cgaggcgttg taccacatta tcgaaaatgc
    21661 gggaaatcgc tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat
    21721 tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt
    21781 cccaccgttt gcgggctttc gcgttgtcga aagtagctgc tactacggca aaggatatca
    21841 ggacgtagag catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc
    21901 gaaaattgat atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga
    21961 tcttacggat aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tgcctttcgt
    22021 ggcacccgtg aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc
    22081 agcatttttt tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag
    22141 ccacagtttt tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt
    22201 ttactggcag gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt
    22261 cgtgaagcgg ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa
    22321 gcccgtagcg gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc
    22381 actctggaag agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc
    22441 gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt
    22501 ggggccatac cgttccgtcc actgcttgaa tcaggaaacc ctggcactgc gcaaattccg
    22561 gtgaccttac ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac
    22621 ggttggttac tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca
    22681 gaagttgaag gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag
    22741 gaaggcgtga cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa
    22801 gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt
    22861 agtcgctgat gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt
    22921 acctgttacc gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa
    22981 tcagtcgaga aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt
    23041 atttcgaaaa acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg
    23101 cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg
    23161 tgacctggtt tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa
    23221 tttatctctc attgtttatt gtctatgcca tcggcactta tgccgtgctc gatccgttta
    23281 ttgcattctg gctggtggcg ggaatgtgca gcttctggct ggcaatgcag gcacagacgt
    23341 ggaaaggcaa aagcgcagga tttttactgc tgggaatcac ctgcggcatg ggggtgatga
    23401 ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc
    23461 aaaaacgctg gaaagatctc tttatttacg gctggctggc ggttatcagt tgcgtactga
    23521 cggttctccc ctggggactg gcgatagcgc agcgggagcc tgacttctgg cactattttt
    23581 tctgggttga gcatattcaa cgctttgcac tagatgatgc ccaacataga gctccgttct
    23641 ggtactacgt gccggtcatc attgccggta gcctgccgtg gctgggatta ctccccggtg
    23701 cactgtacac aggctggaaa aaccgcaagc attccgcaac cgtctatttg ttgagctgga
    23761 cgataatgcc gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc tatattcttt
    23821 cttgctttgc acctctggca atgctgttgg cgcattacgc gttgctggca gcaaaaaata
    23881 atcctctggc gctgcggatt aatggctgga ttaacatcgc ttttggcgtc actggcatta
    23941 ttgccacatt tgtgatctcc ccctggggac caatgaacac gccggtgtgg caaaccttcg
    24001 agagctataa agtcttttgt gcctggtcga ttttttcgct atgggcattt ttcggctggt
    24061 acaccttaac aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg ctggggctgg
    24121 cgttgctggt aggattttca attcctgaca gagtcatgga aggaaaacat ccgcaatttt
    24181 ttgtcgagat gacacaagaa tcacttcagc caagccgcta tattcttact gatagcgtcg
    24241 gtgttgccgc aggtctggca tggagcctgc aacgcgatga catcatcatg tatcgccaga
    24301 caggtgagtt gaaatacggc cttaattatc cggatgcgaa agggagattt gtcagcggtg
    24361 atgagttcgc aaactggctt aatcaacatc gtcaggaggg gattattaca ctcgtgcttt
    24421 cggttgaccg cgatgaagat atcaacagtc tcgccattcc gcccgcagat gccatcgttc
    24481 gtcaggagcg tctggtgctg attcagtatc gtcccaaatg atctggctaa cattagtctt
    24541 tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc
    24601 gataaacaaa cggcgcaaac atatcgtgct gtggctggga ctggcgctgg cttgtcttgg
    24661 tcttgccatg atgctctggc tgctggtctt gcagaacgta ccggtaggca ttgcctaccc
    24721 gatgttaagt ctgaattttg tctgggtgac gctggctgca gtaaaactgt ggcacgaacc
    24781 ggtatcgccg cgtcactggt gtggggtggc gttcattatt ggtggcattg tgatcctcgg
    24841 gagtacggtg taatgtgcct gatatgggga ttattcagcg tcattattgc ctcagttgcg
    24901 caattaagcc tgggttttgc ggcaagtcat ctgccgccga tgacgcactt atgggatttt
    24961 atcgcggctc tgttagcttt tggcctggat gcccggatac tgttactcgg gctgctgggt
    25021 tatctgcttt ccgttttttg ttggtataaa acgctacata aacttgcgct aagcaaagcc
    25081 tatgccttgc tcagtatgag ttatgtgctg gtatggattg cttcaatggt acttcccggc
    25141 tgggaaggga ctttttcgct gaaagcacta ctgggagtag cctgtattat gagcgggttg
    25201 atgctgattt tcctgcccac gacaaaacaa cgttactgag ttttccctgc cgctgtacac
    25261 tgccgttccc actcatcggg gctataactg cttgccgaga ggactttcac cgtatgcagc
    25321 ttttcacgat taatacaata aagcgcattc tgcaaaggcg agagtaaatc ccccactttc
    25381 acggcgaagt cactcttcac ttcggcgatc atttttatcg cgccgccagc atcgcagagc
    25441 ataagtacat gtctgttatc ttgtttattg cagcacgatt ttttgtgcca gccattccat
    25501 tgcattatcc tgtttgctaa gagttttcac atcaatttac agattaacag ataaactctt
    25561 tatcaacggc aatggaatgg cagaaatgag aagtgaaacg gagggttaat tattgctgac
    25621 gttgcaccca ctcttttagc gcctgacgtg aaattttaat accgccgttt ttcagctccg
    25681 gcggtaaagt cagccagcgc accggttgtt gaaaacgagc cagcttatct ttcacccatt
    25741 cgccaagatc aacggtttgc tggtcatact ccaccaccgc taccggccga tgaccaaact
    25801 ccttgtcggc aacggaaacg ataaacacct gcaaaaccgc aggatgtgca gcaataacac
    25861 gctcgacttc ttccggctga ataccctcac cgccactgaa gaacagattg tctaaacgtc
    25921 cgacaatggt cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg taccagcctt
    25981 cgtcattaac cagtgaaacc agttgcccgt tacgccagta accttctgcc atactggcag
    26041 cccgcagcca cacttcatta ttaacgattt tcacttcccg acccggcagc ggcaaaccaa
    26101 cgtctgccag gccgtcggct tctttcgcac ataccgtgga ggcaaactcg gtcagaccat
    26161 agccgcaaaa gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg accgggatag
    26221 ccgcgccgcc aagtaacacc gctttcaggg aaacggaact gcggttaacc agcaaacgcc
    26281 agagttgtgt tggcaccagt gaagcgtgag tacagcctgc cagcatttgc tccaatggct
    26341 gtttatcacg caccgtcatc cgcgcaccag cgtataacca gcgccacata attccctgac
    26401 cggagacgtg aaacagcggt aaagagagca accagtcatc gtgatcgcca aacggaatca
    26461 gcgataacac accttgcgca ctggcaagat gggcctgata agtatggaca gcggctttcg
    26521 gcaaaccggt agaacccgag gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag
    26581 cggcatgtac gccttcaacc agctgaatgt gcagcgacgt taatgccgga aacgtgtttt
    26641 ccccatccgg caccagcgca aattgcagcg tcagattggg cagcaattct tcaagcaacg
    26701 gttgcggcag ctgagggttc acgggcaaca cccgcgcccc gcattgcagt aacgccagcc
    26761 aggcgagcag cgtttgcggc gtattccacg cccgcaacat cacgccgctg ccctcaacca
    26821 ccccctgcgc cgcaaagccc gtagctaact catcgacgcg agcacaaagc tcgcgccagt
    26881 tgagttgctc gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac
    26941 gccacggcca gtcagagaag atcataacaa ctgctcctgt acatcagcgt ccactaacgg
    27001 cagtgggctc cccggccagc gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag
    27061 tcccggaatg gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg ttagacctaa
    27121 gctcgactca atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac
    27181 ctgctcgcgt actttttcca gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac
    27241 gccctcttca gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc
    27301 aatgccggtt tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg gctcttcgag
    27361 aaacgcgatg cggtggcgat aatccgggtt aacatatttg gcaaactgct gacctttcag
    27421 cggtgtccag gcgcgatttg cgtcaagacg caaatgcaga tccggaattg cctccagcaa
    27481 cagattcacc accatgccgt cgcgcaccgc ttcgtacaat cccactttga ccttcgccac
    27541 tttctcgccc ggcatatcgg caagtttgag gatcagatcg tccggatcgc cattacacag
    27601 cggtgccgca cggtagttgg ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca
    27661 gcttacgcca aaggccacgg aaggcatctg cggtagctcg caatcgcctg ccagccagtt
    27721 atttacccag gcaagcagca cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg
    27781 cagtggggag atctcccccc acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc
    27841 gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca
    27901 gcggtatacc tgcgcgctac gcattacgga ttccgtttaa atttgctgaa atcaggctga
    27961 cgtttctggt tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg
    28021 gtggcgttgc ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca
    28081 gctttcaggc agcgcagcgc catcgggctg ttttgcagca tttcgcggca ccaacggacg
    28141 gtttcttttt ccagatccgc cagcggtacc acggtgttga caaggcccat atccagcgcc
    28201 tgttttgcgt cgtactgacg gcacaggaac cagatttctc gcgctttttt ctgcccgacg
    28261 atacgagcca tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt cgggccagtc
    28321 tgaccgaaga tggcattatc tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga
    28381 ccaccgccga tggagtagcc agccaccatc gcgacaaccg gtttcggaca ggtacggatc
    28441 tgacgctgga agtccagcac gttcaggtga tgtacgccgg aatcatcttt atagccgccg
    28501 taatcaccac gcactttctg gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc
    28561 agaatgatca cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct
    28621 tttaccgtca gaggacggaa ggcattgcgc acctgcggac gattaatggt gatttttgcg
    28681 ataccgtcgg tggatttttc ataacgaatg tcctcgaaac cttcggagca gtcgtgccat
    28741 tcaaccggtg cgtaaggcat tgcttcatca ggataaatca tattgtgtcc tttagtcaga
    28801 aacgcaagat ctgcgccaga cttgcgatta cgccagcggg attttcccga tgcgcgttat
    28861 gtccggcgcg aggaatgaca tggcagtcgg cagccagttc cgccgccagg gcgcggaatt
    28921 tgctgtcacg ttcaccacat aaataataaa acgcaaatgt gcgggcgcta aggttagcac
    28981 gtaaatcagg ctggacggcg agagaagtcg cctccagcat ggcggcaagg gttgcgccat
    29041 tattgttgct gcgcagcgcc accagctccc ggcgttgatc gtcattgagc gaggcaaaaa
    29101 caggctgttg ataccagtcg gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg
    29161 cccattggcg atcggaacgc tgacgttccg cacgttgttc agcattttgc agccccggat
    29221 gcccgccttc gacaataacc ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg
    29281 ccacccgtcc gccaagcgag taccccacca gccagaagtc aaggatgttg taactaacca
    29341 aggttttacg cagtaagtcg gtgacatcat caaatccatc gacgctaatc gccgccgaac
    29401 caccatgacc taggagatca acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt
    29461 cttgccattc gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta
    29521 gttttccgtg ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg
    29581 ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg
    29641 cgtgcgccag gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg gatgatgata
    29701 tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa
    29761 acgctcacgc tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt
    29821 caccacaatt aataccagcg gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc
    29881 gtaaagcgcg gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac
    29941 gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc cctcggttgc tgtacaccgg
    30001 gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt accaccaggc tgttaccaac
    30061 aaacaattgc ccctgttcag gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc
    30121 aaacgcatca cggcgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc
    30181 aacgcaccag ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattggc
    30241 aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat
    30301 ccagtactct tctggttcac agcttgcctg ccattgcagg agccgtttcc ccgtcaggct
    30361 acttcccagt tgcaccacaa tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc
    30421 taaccaaaga tcggcacacg gcagcggctg cccggtttgt gacagcacat cgccaatcag
    30481 cggccagcca agagtttgcg cccacagggc aacttttttg ccctcttccg cactcatgcg
    30541 cccggcaacc accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact
    30601 ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cacccagacg
    30661 ctgttgccag ctaagcccgg tatcgtccat ttcgccatac agcggttcag caaacgggca
    30721 gttgatatgg actccccccg catgaagcgt accgagagcg tggtcgatgg tagaaaccag
    30781 ccaacgtgcg gggatatcct gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga
    30841 ggcgaacatt cccggctggc gaattgcctg attcgcgccg cagtcaatta gctccggcgg
    30901 gcgatcggcg gttaagagaa tcagtttttc tccggttaac ccggcttcaa tcagtgccgg
    30961 atagagattt gccaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac
    31021 tttcgccagc cccagcgcca gatgccccaa cccacgctca tcgaaatggg tgtggtgaat
    31081 gaatgcggaa ttctccgccg ccgctaacgt taacggtgta gaacgcgagc ctggggcgat
    31141 acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg
    31201 gttaaatgcg cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga
    31261 gctacaaaaa tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt
    31321 gtaataaagt acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt
    31381 cggaaccacg gacaatgccc gcgccagcat ataatcgcac gacattgccg ctaatttttg
    31441 ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga gagatagccc gcagaaccgg
    31501 cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg
    31561 gtaagccagc aactgctgcc gttggctgca actgatgtaa acagatcaca tcatccgctt
    31621 tgttgagtga agtccagata cagcggcgaa gatgctgcac tttacgcaga cgcagtacct
    31681 gcggcggtaa aacatccagc gtctgggtat cggcctgtaa tcgttggcag atatcttcca
    31741 ccaccagcat gttctcgcgc tggtttttat catccgccat cagccactct cctaactgct
    31801 gcgcctgctt atcatcagga tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg
    31861 ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc aagaaaagca ttttcgccat
    31921 caaaggccat gtaaaaatgg tagcaattca gattcagtcg acgactggca gccatcatcg
    31981 ccgccgcgtt gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc accactttgt
    32041 cgagctcacc ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt
    32101 tgtccggcca gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac
    32161 tcaccagtgt ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc
    32221 tgaatagcgt cagccgcagc gtggctttac cgccacagcg tcgccattcc aggcggggta
    32281 aaagtaaatt gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt
    32341 gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg
    32401 cgcccaggac ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcgggtacg
    32461 tttgctgact ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat
    32521 cgataacccg aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt
    32581 ccagcgccgt agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac
    32641 ggggtactac aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca
    32701 acagtcctag taccagcccg acggccgcgc ccacaccaat tccttgccag ggtttttcgt
    32761 ggacgtagtc atcagcacga taaacagcct gcttcgcccg atagtaataa ctgtctgaag
    32821 cctggctaac ccgttttttc acatcatcca gcgctttttc tgcatgcgct ttcagctcaa
    32881 catatttctg atcggcggga tcgccagagg agcggagcac ctcttccagt gtttcactaa
    32941 gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg attagacatt ctcgttctcc
    33001 atttgcgtaa aacctgtcac taactataga caagagttgt tgattacgcc tgaattacct
    33061 cgcgtgccat cccaatgtgt gggataccat cttcttcata gacctccgtc acagggataa
    33121 aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat
    33181 caggccagtg atgcgtacat gtttcgagtg ttttactcat cagctgctgc cccacttttt
    33241 caccacgcaa cgcttcactg acaatcaccc gacctataac gaccggctca agatcatcat
    33301 cacttttcag aatcctcgca tacgccacca gttcatcatt tttccaaccg agaatatggc
    33361 gattatcccc cgtcaaatca tcaccatcga tatcctgata agggcaattc tgctcgacaa
    33421 caaacaccgc gcaacgcagt tgtaataagg cataaagctg ggaaaccgat aattcagagt
    33481 gatgcagatc ttgccattca atcataatgc tctcctttta gatgtatgcc ttatactagc
    33541 cggaatattt tttgaaacgt gatgaactca tggaattaat ttttttaggt acttcagccg
    33601 gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct taacctgcaa catccgaccc
    33661 agagcggact ttggttgttt gactgcggtg aaggcaccca gcatcagcta ctgcataccg
    33721 cctttaaccc tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatcttt
    33781 ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga
    33841 tttatggtcc gcaaggtatc cgtgaatttg tggaaaccgc gctgcggatt agcggctcat
    33901 ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc
    33961 tgcgcaaagt aaccgcttat ccgctggaac acccactgga atgttatggc tatcgtattg
    34021 aagaacatga taaaccgggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc
    34081 ctggcccact gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc
    34141 agattaacgg cgcagattac ttagctgctc cagtgccagg taaagcgctc gctattttcg
    34201 gcgataccgg cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc
    34261 acgaagcgac gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta
    34321 cacgccaggc tgcgacacta gcccgtgagg ctggagtcgg caagctaatc attacccacg
    34381 tcagctcgcg ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt
    34441 tcccggcgac tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat
    34501 taaaaacagg gtgaaacttg ccctgttata gcaacccgcg ctacatatat ccacccacac
    34561 aggcatattg agcgattttt gatatttatt catctcctta cgcactatat tcagagcaat
    34621 aagttgcctc ttttcatctt gattcagcac aacctttgca actaaaagga gcccatgatg
    34681 gttacagttg tcagcaatta ttgtcaatta tctcaaaccc aactcagtca gacatttgca
    34741 gaaaaattta ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga
    34801 gatgaagaaa gtattgagtt actgcataat attgcgttag gttatgatga atttggaaaa
    34861 aaagctgggt aatgctgcca acttactgat ttagtg
//
LOCUS       DAJTNP010000035        34801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTNP010000035 DAJTNP010000000
VERSION     DAJTNP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..2098
                     /locus_tag="OO160_001857"
     CDS             <1..2098
                     /locus_tag="OO160_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5524003.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO160_001858"
     CDS             2095..3183
                     /locus_tag="OO160_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5524004.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO160_001859"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO160_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5524005.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO160_001860"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO160_001860"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5524006.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO160_001861"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO160_001861"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5524007.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO160_001862"
     CDS             8051..8602
                     /locus_tag="OO160_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5524008.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO160_001863"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO160_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5524009.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO160_001864"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO160_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5524010.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO160_001865"
     CDS             complement(11683..12681)
                     /locus_tag="OO160_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5524011.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO160_001866"
     CDS             complement(12760..13452)
                     /locus_tag="OO160_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5524012.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO160_001867"
     CDS             complement(13529..14032)
                     /locus_tag="OO160_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5524013.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO160_001868"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO160_001868"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5524014.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO160_001869"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO160_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5524015.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO160_001870"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO160_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5524016.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO160_001871"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO160_001871"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5524017.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO160_001872"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO160_001872"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5524018.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO160_001873"
     CDS             complement(20922..21341)
                     /locus_tag="OO160_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524019.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO160_001874"
     CDS             complement(21319..21672)
                     /locus_tag="OO160_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524020.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO160_001875"
     CDS             complement(21673..21993)
                     /locus_tag="OO160_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524021.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO160_001876"
     CDS             complement(22127..23560)
                     /locus_tag="OO160_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5524022.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO160_001877"
     CDS             complement(23623..24072)
                     /locus_tag="OO160_001877"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5524023.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO160_001878"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO160_001878"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5524024.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO160_001879"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO160_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5524025.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO160_001880"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO160_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO160_001881"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO160_001881"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5524026.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO160_001882"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO160_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO160_001883"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO160_001883"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5524027.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO160_001884"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO160_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO160_001885"
     tRNA            complement(34082..34157)
                     /locus_tag="OO160_001885"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO160_001886"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO160_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8488 a         8949 c         9145 g         8219 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgct tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
    34801 g
//
LOCUS       DAJTNP010000036        34447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTNP010000036 DAJTNP010000000
VERSION     DAJTNP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..334)
                     /locus_tag="OO160_001887"
     CDS             complement(<1..334)
                     /locus_tag="OO160_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524028.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="OO160_001888"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="OO160_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5524029.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="OO160_001889"
     CDS             complement(1695..2075)
                     /locus_tag="OO160_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5524030.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="OO160_001890"
     CDS             complement(2089..2505)
                     /locus_tag="OO160_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524031.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO160_001891"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO160_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5524032.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="OO160_001892"
     CDS             complement(3506..3973)
                     /locus_tag="OO160_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5524033.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="OO160_001893"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="OO160_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5524034.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="OO160_001894"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="OO160_001894"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5524035.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="OO160_001895"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="OO160_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5524036.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO160_001896"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO160_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5524037.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="OO160_001897"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="OO160_001897"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5524038.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="OO160_001898"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="OO160_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5524039.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="OO160_001899"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="OO160_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5524040.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO160_001900"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO160_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5524041.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="OO160_001901"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="OO160_001901"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5524042.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="OO160_001902"
     CDS             complement(14628..15443)
                     /locus_tag="OO160_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5524043.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="OO160_001903"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="OO160_001903"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5524044.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="OO160_001904"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="OO160_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5524045.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="OO160_001905"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="OO160_001905"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5524046.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO160_001906"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO160_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5524047.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="OO160_001907"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="OO160_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5524048.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="OO160_001908"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="OO160_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5524049.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="OO160_001909"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="OO160_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5524050.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22786
                     /gene="tatC"
                     /locus_tag="OO160_001910"
     CDS             22010..22786
                     /gene="tatC"
                     /locus_tag="OO160_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5524051.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22828..23610
                     /gene="tatD"
                     /locus_tag="OO160_001911"
     CDS             22828..23610
                     /gene="tatD"
                     /locus_tag="OO160_001911"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5524052.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO160_001912"
     CDS             complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO160_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5524053.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24262..25755
                     /gene="ubiD"
                     /locus_tag="OO160_001913"
     CDS             24262..25755
                     /gene="ubiD"
                     /locus_tag="OO160_001913"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5524054.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25801..26502
                     /gene="fre"
                     /locus_tag="OO160_001914"
     CDS             25801..26502
                     /gene="fre"
                     /locus_tag="OO160_001914"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5524055.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO160_001915"
     CDS             complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO160_001915"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5524056.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO160_001916"
     CDS             complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO160_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5524057.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30246..31577
                     /gene="pepQ"
                     /locus_tag="OO160_001917"
     CDS             30246..31577
                     /gene="pepQ"
                     /locus_tag="OO160_001917"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5524058.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31577..32191
                     /locus_tag="OO160_001918"
     CDS             31577..32191
                     /locus_tag="OO160_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5524059.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32230..33681
                     /gene="trkH"
                     /locus_tag="OO160_001919"
     CDS             32230..33681
                     /gene="trkH"
                     /locus_tag="OO160_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5524060.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33693..34238
                     /gene="hemG"
                     /locus_tag="OO160_001920"
     CDS             33693..34238
                     /gene="hemG"
                     /locus_tag="OO160_001920"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5524061.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8701 c         9302 g         8336 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tccttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgccctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    22801 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    22861 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    22921 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    22981 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    23041 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    23101 tgtggtctcg actttaaccg taacttttcg acgccggaag agcaggaacg cgcttttgtt
    23161 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    23221 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    23281 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    23341 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    23401 ttgattccgg cggagaaatt gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat
    23461 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    23521 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    23581 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    23641 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    23701 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    23761 ggataagggg gggccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatt
    23821 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca caaagtggct gacgccacgc
    23881 gtagcgctga tggtcgtggt atgaatcact tctggatcaa attccacaaa caggtagtta
    23941 gggaataacg gttcactgac tgcggtacgt ttaccccgta cgattttttc cagggtgatc
    24001 atcggagcga ggcaattaac cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc
    24061 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    24121 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    24181 gtcagagttc acgctaattt aacaaattta cagcattgca aagatgaacg ccgtataatg
    24241 ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcctg
    24301 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    24361 gaaatcactg aaattgctga ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa
    24421 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    24481 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    24541 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    24601 tttaagcagg tgttaaacat gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa
    24661 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    24721 gatgccgcgc cgctgattac ctgggggctg accgtaacgc gcggcccgca taaagagcgg
    24781 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    24841 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    24901 cgtttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc
    24961 cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg caccaaaacc
    25021 gaagtagtaa agtgtatttc caatgacctc gaagtgccag ccagtgccga aatcgtcctg
    25081 gaagggtata tcgatcctgg tgagatggcg ccggaagggc cgtatggtga ccacacaggt
    25141 tactataatg aagtcgataa tttcccggtg tttaccgtga cgcatattac ccagcgtgaa
    25201 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    25261 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    25321 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    25381 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    25441 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    25501 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    25561 cctattgatt atctggattt tgcctcgcct gtctctgggc tgggttcaaa aatggggctg
    25621 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    25681 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    25741 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    25801 atgacaacct taagctgtaa agtgacctcg gtagcagcta tcacggatac cgtatatcgt
    25861 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    25921 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    25981 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    26041 atcctcaaag atcatcaaat cgtggtcgac cttccccacg gagaagcgtg gctgcgcgat
    26101 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    26161 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    26221 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcacctt
    26281 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    26341 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    26401 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    26461 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    26521 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    26581 catccggcaa ttggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    26641 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26701 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    26761 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26821 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    26881 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    26941 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    27001 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    27061 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    27121 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    27181 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    27241 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    27301 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    27361 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    27421 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    27481 atcggcacat ggcctatatg ctccacgccg ccaaccagac atgcctgcgt atcgccagtc
    27541 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    27601 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatgtta
    27661 aaaccctgct ccagcgtctg ttgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    27721 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27781 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27841 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27901 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    27961 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    28021 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    28081 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    28141 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    28201 ttcggttggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    28261 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    28321 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
    28381 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    28441 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    28501 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    28561 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    28621 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28681 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    28741 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    28801 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    28861 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    28921 gagatcacgc cagccagttt cagaccatcg attttgccgc gctcaagctg cttgttcagc
    28981 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    29041 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    29101 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    29161 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    29221 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    29281 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc
    29341 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    29401 tgcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    29461 gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag accgattttc
    29521 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagtgc actgtcagcg
    29581 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    29641 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29701 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    29761 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29821 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29881 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29941 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    30001 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    30061 gtctcctgaa tccgcaaggt aatctggtcg taccagatgc atcggagtgt gtgttttgtg
    30121 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    30181 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    30241 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    30301 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    30361 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    30421 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    30481 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    30541 tctggactga agatgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    30601 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30661 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattattacc
    30721 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30781 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaata
    30841 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    30901 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30961 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    31021 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    31081 atgaacaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccaca
    31141 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    31201 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    31261 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacac
    31321 acctcgcggc accggcaaaa tatccgtacc tgcgctacac ccgtattctc cagccgggca
    31381 tggtgttaac catcgaaccg ggtatctact tcattgaatc gcttctggca ccgtggcgtg
    31441 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    31501 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    31561 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    31621 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    31681 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31741 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31801 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    31861 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31921 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    31981 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    32041 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    32101 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    32161 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    32221 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    32281 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    32341 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    32401 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
    32461 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    32521 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    32581 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    32641 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32701 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32761 attgcggaaa cggcgaaaac tctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32821 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32881 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagtccgact
    32941 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    33001 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    33061 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    33121 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    33181 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    33241 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    33301 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    33361 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    33421 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    33481 acgggcgtgg atgatttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    33541 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    33601 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattactggt gctctttacc
    33661 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33721 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33781 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33841 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33901 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    33961 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    34021 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    34081 gttatcgctg gtacgaccgt tttatgctca agctgattat gaagatgtca ggcggtgaaa
    34141 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    34201 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    34261 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    34321 accactgaca cggaacaacg gcaaacacgc agccgggtca gcggggttct cctgagaatt
    34381 ccgacagaga aagcaaaaat aaatgcttga ctttgtagcg ggaaagcgta ttatgaatac
    34441 cccgcgc
//
LOCUS       DAJTNP010000037        33511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTNP010000037 DAJTNP010000000
VERSION     DAJTNP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO160_001921"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO160_001921"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5524062.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO160_001922"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO160_001922"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5524063.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO160_001923"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO160_001923"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5524064.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO160_001924"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO160_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5524065.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO160_001925"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO160_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5524066.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO160_001926"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO160_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5524067.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO160_001927"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO160_001927"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5524068.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO160_001928"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO160_001928"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5524069.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO160_001929"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO160_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5524070.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO160_001930"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO160_001930"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5524071.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO160_001931"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO160_001931"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5524072.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO160_001932"
     CDS             8212..9075
                     /locus_tag="OO160_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5524073.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO160_001933"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO160_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5524074.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO160_001934"
     CDS             10411..11028
                     /locus_tag="OO160_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5524075.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO160_001935"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO160_001935"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5524076.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO160_001936"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO160_001936"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5524077.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO160_001937"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO160_001937"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5524078.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO160_001938"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO160_001938"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5524079.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO160_001939"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO160_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5524080.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO160_001940"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO160_001940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5524081.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO160_001941"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO160_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5524082.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO160_001942"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO160_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5524083.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO160_001943"
     CDS             21859..23568
                     /locus_tag="OO160_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5524084.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO160_001944"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO160_001944"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5524085.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO160_001945"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO160_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO160_001946"
     tRNA            27625..27719
                     /locus_tag="OO160_001946"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO160_001947"
     CDS             27991..29202
                     /locus_tag="OO160_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5524086.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO160_001948"
     CDS             29614..30657
                     /locus_tag="OO160_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5524087.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="OO160_001949"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="OO160_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="OO160_001950"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="OO160_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32846..33494
                     /locus_tag="OO160_001951"
                     /pseudo
     CDS             32846..33494
                     /locus_tag="OO160_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8398 a         8808 c         8257 g         8048 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcaa aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg t
//
LOCUS       DAJTNP010000038        33177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTNP010000038 DAJTNP010000000
VERSION     DAJTNP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(2..94)
                     /locus_tag="OO160_001952"
     tRNA            complement(2..94)
                     /locus_tag="OO160_001952"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO160_001953"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO160_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5524088.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO160_001954"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO160_001954"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5524089.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO160_001955"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO160_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5524090.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO160_001956"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO160_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5524091.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO160_001957"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO160_001957"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5524092.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO160_001958"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO160_001958"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5524093.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO160_001959"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO160_001959"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO160_001960"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO160_001960"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5524094.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO160_001961"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO160_001961"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5524095.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO160_001962"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO160_001962"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5524096.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO160_001963"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO160_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5524097.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO160_001964"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO160_001964"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5524098.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO160_001965"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO160_001965"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5524099.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO160_001966"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO160_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5524100.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO160_001967"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO160_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5524101.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO160_001968"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO160_001968"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5524102.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO160_001969"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO160_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5524103.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO160_001970"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO160_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5524104.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO160_001971"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO160_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5524105.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO160_001972"
     CDS             complement(20900..21019)
                     /locus_tag="OO160_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5524106.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO160_001973"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO160_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5524107.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO160_001974"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO160_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5524108.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25529)
                     /gene="hycC"
                     /locus_tag="OO160_001975"
     CDS             complement(23682..25529)
                     /gene="hycC"
                     /locus_tag="OO160_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5524109.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLY
                     HLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPL
                     NGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAP
                     RTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQP
                     MITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQA
                     FAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25566..26177)
                     /gene="hycB"
                     /locus_tag="OO160_001976"
     CDS             complement(25566..26177)
                     /gene="hycB"
                     /locus_tag="OO160_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5524110.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26302..26763)
                     /gene="hycA"
                     /locus_tag="OO160_001977"
     CDS             complement(26302..26763)
                     /gene="hycA"
                     /locus_tag="OO160_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5524111.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26975..27325
                     /gene="hypA"
                     /locus_tag="OO160_001978"
     CDS             26975..27325
                     /gene="hypA"
                     /locus_tag="OO160_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5524112.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27329..28201
                     /gene="hypB"
                     /locus_tag="OO160_001979"
     CDS             27329..28201
                     /gene="hypB"
                     /locus_tag="OO160_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5524113.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28192..28464
                     /gene="hypC"
                     /locus_tag="OO160_001980"
     CDS             28192..28464
                     /gene="hypC"
                     /locus_tag="OO160_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5524114.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28464..29585
                     /gene="hypD"
                     /locus_tag="OO160_001981"
     CDS             28464..29585
                     /gene="hypD"
                     /locus_tag="OO160_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5524115.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29582..30592
                     /gene="hypE"
                     /locus_tag="OO160_001982"
     CDS             29582..30592
                     /gene="hypE"
                     /locus_tag="OO160_001982"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5524116.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30666..32744
                     /gene="flhA"
                     /locus_tag="OO160_001983"
     CDS             30666..32744
                     /gene="flhA"
                     /locus_tag="OO160_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5524117.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32781..33134)
                     /locus_tag="OO160_001984"
     CDS             complement(32781..33134)
                     /locus_tag="OO160_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5524118.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7815 a         9261 c         8631 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc accactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag ccccagcccc
    24661 agccccagcc ccagcaggat aatgccgata ttttccaggg tgtggtaagc cagcaggcgc
    24721 tggatgttgt gttccatcaa cgcatacaga ccaccgacga acgcggtgat catgccgagc
    24781 accagcagcg cgatccccca ccacagcggt gcattaccgc ccagcagtga cagggttaac
    24841 atgcccagca ggccaatttt catgactacc gtagaaaaca acgcggcagc tggcgcagag
    24901 gcgttagcat gtgcctgcgg cacccagccg tgcagcggaa taatcccagc cagcaggcca
    24961 aagccaatca caccgagcag ccagatatcg gaaccgagcg gcagctgttg catacgcata
    25021 tccagcaggc gcagatccag cgtgccataa cgctgccaca gcagccagca agcaatcacc
    25081 agcagcagag tgccaagacg cctcagcgca aaccacagtt tgccctcttt gctgttgctg
    25141 gtgaggaaca ccgcacacaa ggccatgact tcggccatta ccacgaacat gccgaggttg
    25201 ctggcgatga cggcgcagac ggcggcagcc atcaacatat tgatctgcaa gccgttgcac
    25261 ttcacctgcg cgtggcgatg ccagtcaatg ttatagaggc tgacaaacag accgcaaagg
    25321 ccgagggtaa tcagccaaat cgcgttaagc ggggagattt gcacatcgta gcttaccagc
    25381 gacagcgcac cgctcacgcc aaccgcgcca gtcagtacag tgaagcccgc ggctgccgta
    25441 tacagactac caaccgcgcc gccaattccg gctatccagc cacttagcgc tttttgaaaa
    25501 gagaaatgcc agaacagcgg cggcgacaaa ccatgccacg ccgctattga tcagggaaat
    25561 tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc gccaaagtcc
    25621 gtgttaaagg tcagctcacg cttacgtttg ctgacgcggg cgatatcggt gttatccacc
    25681 agatgcaggg ctttagtcgg gcacatccgc acacaggccg gaccttgttc atcaaagcta
    25741 caaaggtcac atttgacggc gatcgcgcga atacctggca cccagtcaag caatgtgctg
    25801 acacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggcgtt tgccggaata
    25861 tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat gccgcacagt
    25921 ttgcagctta cgcacagact ttcattcaac tgcacggccc catcaacgcg ggtgatggcg
    25981 ttaaccgggc agaccaccgc gcagggtgca tcttcacagt ggtgacagag ctgcggcgca
    26041 gattcttttt cattgagcat cactctcagg cgcggcattg attgcaggcc gtgctggcga
    26101 tgcgtctctg aacaggcggc ctcacaggtg tggcagccga tacagagcgt ggagtcggca
    26161 attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg aaaacatgcc
    26221 gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc gtaacgcctg
    26281 caaaacgggc aaagcctcag ctcatgctgc cgggctttgt ccctttacca gttggcttaa
    26341 attcaccggc acattgtttt caatggcggt gtataagctg tcgtaaacgc cagcgatttt
    26401 ttcgagatag tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc catcaatcat
    26461 gccgtcaatg ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat cttttgtttc
    26521 gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt aaatacgaaa
    26581 atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg cgtgatgcag
    26641 gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt gccactggtc
    26701 ctgtaggcga cgatgccgct gtgcgatgta atcggctttc tcgcttattt cccaaatagt
    26761 cattgtcagg ttactcgttt aacagagatg ccagctttaa gcattttttg tgccaacttt
    26821 taatttattg ttattaaaga gatttttaag ctaaagatga atttcgtcgc cgtgtcgacg
    26881 tgtcatttcg acatcatcga cattattcac cgcagggata atcaacactg gcgcaattat
    26941 tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc tctgccaacg
    27001 ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg taactggggt
    27061 ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct tttgttttga
    27121 tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag aacaagaggc
    27181 cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc gcgtccgccg
    27241 ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt tacagattcg
    27301 gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg gtgaaggcaa
    27361 cctgtatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg cgccatttgc
    27421 cccggcggca cgcccgcaga tgaaaatcac cggcattaaa gcgcctgaat ttacccccag
    27481 ccagactgaa gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc acacaccggg
    27541 catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa acaaccgtct
    27601 ggctgaacgc aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca acctggtttc
    27661 cagccctggt tccggtaaaa ccaccctgct gacggaaacc ttaatgcgcc tgaaagacag
    27721 cgttccgtgc gcggttattg aaggcgacca gcaaaccgtg aacgatgccg cacgcattcg
    27781 cgctaccggt acaccagcga ttcaggtgaa caccggtaaa ggctgccatc ttgacgcaca
    27841 gatgattgcc gacgccgcac cgcgtctgcc actggacgat aacggtattc tgtttatcga
    27901 aaacgttggc aacctcgtat gcccggccag cttcgatctc ggtgaaaaac acaaagtagc
    27961 ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcata tgtttgccgc
    28021 cgcctcgctg atgctgctca acaaagttga cctgttgccg tatctcaact ttgacgttga
    28081 gaagtgcatc gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc ttatttccgc
    28141 caccagcggc gaagggatgg accagtggct gaactggctg gagacacagc gatgtgcata
    28201 ggcgttcccg gccagatccg caccattgac ggcaaccagg cgaaagtcga cgtctgcggc
    28261 attcagcgcg atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg tcagccgcgc
    28321 gtgggccagt gggtactggt tcacgttggc tttgccatga gcgtaattaa tgaagccgaa
    28381 gcacgcgaca ctctcgacgc cttacaaaac atgtttgacg ttgagccgga tgtcggcgcg
    28441 ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg ccggaacagg
    28501 tgatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac accgccgaac
    28561 gccctctgcg gattatggaa gtgtgtggcg gtcataccca cgccattttt aaattcggcc
    28621 tcgaccagtt actgcctgaa aacgttgagt ttatccacgg tccgggttgc ccggtgtgcg
    28681 tactgccgat gggtagaatc gacacctgcg tggagattgc cagccatccg gaagtcatct
    28741 tctgtacctt tggcgacgcc atgcgcgtac cagggaaaca gggatcgctg ttgcaggcaa
    28801 aagcacgcgg tgccgatgtg cgcatcgttt actcgccgat ggatgcgttg aaactggcgc
    28861 aggagaatcc aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc accatgccga
    28921 ccaccgccat cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt tacttcttct
    28981 gccagcacat tacgcttatc ccgacgctgc gcagtttgct ggaacagccg gataacggta
    29041 tcgacgcgtt cctcgcgccg ggccacgtta gtatggttat cggcactgat gcctataatt
    29101 ttatcgccag cgattttcat cgtccgctgg tggtcgctgg tttcgaaccc cttgatctac
    29161 tgcaaggcgt ggtcatgctg gtggagcaga aaatagcggc ccacagcaag gtagagaatc
    29221 agtatcgtcg ggtggtaccg gatgccggta acctactggc gcaacaggcg attgccgatg
    29281 tgttctgtgt caacggcgac agcgaatggc gcggcttagg cgtgattgaa tcttctggcg
    29341 tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc ccggcaccgc
    29401 agcaggtctg cgatgacccg cgcgcgcgtt gtggtgaggt attaacgggc aaatgtaagc
    29461 cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcattt ggtgcactga
    29521 tggtctcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag gagagtgaag
    29581 cgtgaataat atccaactcg cccacggcag cggcggccag gcgatgcagc aattaatcaa
    29641 cagcctgttt atggaagcct ttgccaaccc gtggctggca gagcaggaag atcaggcacg
    29701 tcttgatctg gcgcagctgg tagcggaagg cgaccgtctg gcgttctcca ccgacagtta
    29761 cgtcattgac ccgctgttct ttcctggcgg taatatcggc aagctggcga tttgcggcac
    29821 cgcgaatgac gttgcgggca gtggcgctat tccgcgctat ctctcctgtg gctttatcct
    29881 cgaagaagga ttgccgatgg agacactgaa agccgtagtg accagcatgg cagaaaccgc
    29941 ccgcacggca ggcattgcca tcgttactgg cgatactaaa gtggtgcagc gcggcgcggc
    30001 agataaactg tttatcaaca ccgctggcat gggcgcaatt ccggcgaata ttcactgggg
    30061 cgcacagacg ctaaccgcag gcgatgtatt gctggttagc ggtacactcg gcgaccacgg
    30121 ggcgactatc cttaacctgc gtgagcagct ggggctggat ggcgaactgg tcagcgactg
    30181 cgcggtgctg acgccgctta ttcagacgct gcgtgacatt cccggcgtga aagcgctgcg
    30241 tgatgccacc cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag cctgcggttg
    30301 tggtattgaa atttctgaat cagcgctgcc ggttaaacct gccgtgcgcg gcgtttgcga
    30361 attgctggga ctggacgccc tgaactttgc caacgaaggc aaactggtga tcgccgttga
    30421 acgcaacgcg gcagagcaag tgctggcagc gttacattcc catccactgg ggaaagacgc
    30481 ggcgctgatt ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc tgtatggcgt
    30541 gaaacgaacc ctcgatttac cacacgccga accgcttccg cgtatatgct aataaaattc
    30601 taaatctcct atagttagtc aatgaccttt tgcaccgctt tgcggtgctt tcctggaaga
    30661 acaaaatgtc atatacaccg atgagtgatc tcggacagca agggttgttc gacatcactc
    30721 ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg caactggtaa
    30781 agcgttctgc gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag actcaacgtg
    30841 cgtcttatta cgcgtcgcgt gaaaaagaca cccccattaa atatgaagac gaaactgttc
    30901 tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgctgcat tgcagttacg
    30961 aagaattttg tgaaacctgg ccgcagctgg ccgcaggtgg gctatacccc aaatttggtc
    31021 actattgcct gatgccactg gcggcgggag ggcatatttt tggtggctgt gaatttattc
    31081 gttatgacga gcgtccctgg agcgaaaaag agttcaatcg tctgcaaaca tttacgcaga
    31141 tcgtttctgt cgtcaccgaa caaatccaga gccgcgtcgt caacaatgtc gactatgagt
    31201 tgttatgccg ggagcgcgat aacttccgca tcctggttgc cattaccaac gcggtgcttt
    31261 cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccat tactatttcg
    31321 acattgacga tatcagcatt gtcttacgca gccaccgtaa aaacaaactc aacatctact
    31381 ccactcacta tcttgataaa caacatcccg cccacgaaca gagcgaagtc gatgaagccg
    31441 gaaccctcac cgaacgcgtg ttcaaaagta aagagatgct gctgatcaat ctctacgagc
    31501 gggacgattt agccccctat gaacgcatgt tgttcgacac ctggggcaac cagattcaaa
    31561 ccttgtgcct gttaccgctg atgtctggcg acaccatgct gggcgtgctg aaactggcgc
    31621 aatgcgaaga gaaagtgttt accactacca atctgaattt gctgcgccag attgccgaac
    31681 gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aatccatcgt ctgaaagaac
    31741 ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat agtgaatttg
    31801 gcgagattat tggccgcagc gaagccatgt acagcgtgct taaacaagtt gaaatggtgg
    31861 cgcaaagtga cagtaccgtg ctgatcctcg gtgaaactgg ctcgggtaaa gagctgattg
    31921 cccgtgcgat ccataatctc agtgggcgta ataatcgccg catggtcaaa atgaactgcg
    31981 cggcgatgcc tgccggattg ctggaaagcg atctgtttgg tcatgagcgt ggggctttta
    32041 ccggtgccaa cgcccagcgc attggtcgtt ttgaactggc ggataaaagc tctctgttcc
    32101 tcgacgaagt gggcgatatg ccactggagt tacagccgaa gttgctgcgt gtattgcagg
    32161 aacaggagtt tgaacgtctc ggcagcaaca aaatcattca gacggacgta cgtttaattg
    32221 ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg agagttccgt agcgatctct
    32281 attaccgcct gaacgtattc ccgattcacc tgccgccact acgcgagcgt ccggaagata
    32341 ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg cgcaatatcg
    32401 acagcattcc tgccgagacg ctgcgcacct tgagcaacat ggagtggccg ggtaacgtac
    32461 gcgaactgga aaacgtcatt gagcgcgcag tattgctaac acgcggcaac gtgctgcagc
    32521 tgtcattgcc agatattgct ttaccggagc ctgaaacgcc gcctgccgca acggttgtcg
    32581 cccaggaggg cgaagatgaa tatcagttga ttgtgcgcgt gctgaaagaa accaacagcg
    32641 tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg accctgctgt
    32701 cacggatgaa gcggctggga attgataaat cggcattgat ttaactgcaa attgccggac
    32761 agatctgcct gtccggcata ctattcacga ggttttttcg gacgatattt ttccggcagt
    32821 tctggcaccg gacgcttgtc atcgatgaga tgacgcacgg ttaagatcgg atgacgccac
    32881 agcattctcg gcccggccca acgcataatc tgtttcatct cttcacgctt tgcaggctgg
    32941 taacagtgca ccggacactg cttacaggct ggtttctctt cgccgaacac acatttatcc
    33001 agccgctttt gcgcgtaaac aaacaacgcc tcgtaatgct ccggctccgc tgacgcctgc
    33061 gggcatttcg cttgataaag atcgatcatt tttttaatcg tcagtttttc acgagagata
    33121 cgcttgccgg acatgctgcc tccacctcat taagatgcat ttatattaca tcttaat
//
LOCUS       DAJTNP010000039        30516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTNP010000039 DAJTNP010000000
VERSION     DAJTNP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            38..1696
                     /locus_tag="OO160_001985"
     CDS             38..1696
                     /locus_tag="OO160_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5524119.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1693..2649)
                     /locus_tag="OO160_001986"
     CDS             complement(1693..2649)
                     /locus_tag="OO160_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5524120.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2800..3915
                     /gene="macA"
                     /locus_tag="OO160_001987"
     CDS             2800..3915
                     /gene="macA"
                     /locus_tag="OO160_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5524121.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3912..5858
                     /gene="macB"
                     /locus_tag="OO160_001988"
     CDS             3912..5858
                     /gene="macB"
                     /locus_tag="OO160_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5524122.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5931..6155)
                     /gene="cspD"
                     /locus_tag="OO160_001989"
     CDS             complement(5931..6155)
                     /gene="cspD"
                     /locus_tag="OO160_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5524123.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6478..6798
                     /gene="clpS"
                     /locus_tag="OO160_001990"
     CDS             6478..6798
                     /gene="clpS"
                     /locus_tag="OO160_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5524124.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6829..9105
                     /gene="clpA"
                     /locus_tag="OO160_001991"
     CDS             6829..9105
                     /gene="clpA"
                     /locus_tag="OO160_001991"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5524125.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9508..9595)
                     /locus_tag="OO160_001992"
     tRNA            complement(9508..9595)
                     /locus_tag="OO160_001992"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9559..9561),aa:Ser,seq:gga)
     gene            complement(9849..10067)
                     /gene="infA"
                     /locus_tag="OO160_001993"
     CDS             complement(9849..10067)
                     /gene="infA"
                     /locus_tag="OO160_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5524126.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10352..11056)
                     /gene="aat"
                     /locus_tag="OO160_001994"
     CDS             complement(10352..11056)
                     /gene="aat"
                     /locus_tag="OO160_001994"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5524127.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11098..12819)
                     /gene="cydC"
                     /locus_tag="OO160_001995"
     CDS             complement(11098..12819)
                     /gene="cydC"
                     /locus_tag="OO160_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5524128.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12820..14586)
                     /gene="cydD"
                     /locus_tag="OO160_001996"
     CDS             complement(12820..14586)
                     /gene="cydD"
                     /locus_tag="OO160_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5524129.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14709..15674)
                     /gene="trxB"
                     /locus_tag="OO160_001997"
     CDS             complement(14709..15674)
                     /gene="trxB"
                     /locus_tag="OO160_001997"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5524130.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16218..16712
                     /gene="lrp"
                     /locus_tag="OO160_001998"
     CDS             16218..16712
                     /gene="lrp"
                     /locus_tag="OO160_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5524131.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16847..20875
                     /gene="ftsK"
                     /locus_tag="OO160_001999"
     CDS             16847..20875
                     /gene="ftsK"
                     /locus_tag="OO160_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5524132.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21030..21641
                     /gene="lolA"
                     /locus_tag="OO160_002000"
     CDS             21030..21641
                     /gene="lolA"
                     /locus_tag="OO160_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5524133.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21652..22995
                     /gene="rarA"
                     /locus_tag="OO160_002001"
                     /gene_synonym="mgsA"
     CDS             21652..22995
                     /gene="rarA"
                     /locus_tag="OO160_002001"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5524134.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23086..24378
                     /gene="serS"
                     /locus_tag="OO160_002002"
     CDS             23086..24378
                     /gene="serS"
                     /locus_tag="OO160_002002"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5524135.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24618..27061
                     /gene="dmsA"
                     /locus_tag="OO160_002003"
                     /pseudo
     CDS             24618..27061
                     /gene="dmsA"
                     /locus_tag="OO160_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27072..27689
                     /gene="dmsB"
                     /locus_tag="OO160_002004"
     CDS             27072..27689
                     /gene="dmsB"
                     /locus_tag="OO160_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5524136.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27691..28482
                     /locus_tag="OO160_002005"
     CDS             27691..28482
                     /locus_tag="OO160_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5524137.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28518..29144)
                     /locus_tag="OO160_002006"
     CDS             complement(28518..29144)
                     /locus_tag="OO160_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5524138.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29459..>30516
                     /locus_tag="OO160_002007"
     CDS             29459..>30516
                     /locus_tag="OO160_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524139.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNL"
BASE COUNT         7513 a         7517 c         8241 g         7245 t
ORIGIN      
        1 gagcgtaaag gataagtcac atatttctgg aggggatatg attcttgagc gcgttgaaat
       61 tgtgggtttt cgcggtatca accgtttgtc gttgatgctc gaacaaaaca acgtcctgat
      121 tggggagaac gcgtggggta aatccagctt gctggacgcc ttaaccctgc tgctatcgcc
      181 agaatcagat ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa
      241 cgggcgagaa catcatctgc atattatttt gaccttccgc gaatcgctgc caggccgaca
      301 tcgggttcgc cgttatcggc cgctggaagc gtgctggacg ccatgcaccg atggctatca
      361 ccgtattttt tatcgtctgg aaggggagag tgcggaagac ggcagcgtga tgacactgcg
      421 cagttttctc gataaagacg gacatccgat tgatgttgag gatattaacg atcaggcacg
      481 ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat
      541 tcgtaacggc acggtgccaa atgtccccaa tgtggaagtc accgcgcgcc agctcgattt
      601 cctcgcccgt gagttgtcct cacatccgca aaatctctct gatggacaga ttcgtcaggg
      661 actttccgca atggtgcagt tgcttgagca ttatttctct gagcaggggg ctggacaggc
      721 gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa cgaagctggc gctatctgga
      781 catcatcaac cggatgattg accgacctgg agggcgctcg tatcgggtta ttttgctcgg
      841 cctgtttgct actttattgc aggcaaaagg cacattgcga ctggataaag acgcgcgtcc
      901 gttattgcta attgaagatc cagaaacccg cctgcatccg attatgcttt ccgttgcctg
      961 gcatctgttg aatcttttgc ctttacagcg tattgctacc accaactctg gcgagctgct
     1021 ttcgttaact ccagtggaac atgtttgccg cctggtgcgt gaatcttctc gtgttgccgc
     1081 ctggcgtctg gggccaagtg gcttgagcac cgaagacagc cgccgcatct cttttcacat
     1141 tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg ttgctggtgg aaggggaaac
     1201 ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga catcattttg atgccgaagg
     1261 gatcaaggtc attgagtttg cccagtccgg gctaaagcca ctggttaaat ttgcccgccg
     1321 aatggggatt gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc
     1381 tacggtacgc agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt
     1441 accggcgctg gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg
     1501 cgtggcgcaa atcccggaaa atgtaccgat gaatctacgc aaaattatct cgaaagcgat
     1561 ccatcgctct tccaaacccg atcttgccat tgaagtggca atggaggcag gacgtcgtgg
     1621 tgtggactcc gtaccaacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg
     1681 cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat
     1741 ctcatagcgc cgacggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg
     1801 tgctatctgt gctggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgactc
     1861 ccagaacgcg ttgtaatcag catggatctt gccttcttta tcgcgataac gcaggctgcg
     1921 gtaaatatgc gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa
     1981 cagacaggcc gcttccatca cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc
     2041 attctggatt tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat
     2101 cgttcttttc ccctgatatt cacacagggt aaacgtgatc tctgccagag gaataccttc
     2161 gctgttgcgg aacaggattg tgctgtcacc ttctttatcc attgagatca tcatggtcag
     2221 ctcaagcgtg aactgctcac cgtttttgcc ttccagttta gccagttgca gcccgggggt
     2281 attcaaatat aagctgaatt cttccgccga catacacccg cggagtaacg cataatggta
     2341 acgtaacgcc tccagcaatt gcttacggct aagattcgcg gcaaggtaag ggcgatgcag
     2401 acgcacaggc agtcgcggct ggcgcgttaa caacacattg agattaggcc agtgggaaag
     2461 ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg
     2521 gcgaaaacta cagcgatgcc agaatttacc cggccgacac tgtccacgtg ccagactaag
     2581 aaaaagtgac aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga
     2641 catattcatg aaatcaatgg ttatacatga cgtcgatttc accattgcgt atcttaacca
     2701 aacatcaata gtgtgattac taacgtaaat tttagggttt tgttgatatt tcgttgaagt
     2761 taatgacccg gattggcata tggagtattc agaatattta tgaaaaagcg gaaaaccgtg
     2821 aagaagcgtt acgttattgc gctggtgata gtcatcgccg gactgattac gttatggaga
     2881 attcttaacg cacccgtgcc gacttatcag acactgattg tgcgccccgg tgatttacag
     2941 caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta aggttgacgt gggcgcgcag
     3001 gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag
     3061 cttttagggg tcattgatcc tgaacaggct gaaaaccaga tcaaggaggt cgaagcaacg
     3121 ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg
     3181 acgtattccc gtcagcaacg tctggcacaa acgcaagcgg tttcactgca ggatctcgat
     3241 accgccgcga cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc
     3301 aagcgcaatc aggcctctct cgatacggct aaaaccaatc tcgattacac ccgtatcgtt
     3361 gccccgatgg ctggggaagt gacgcaaatc accactctgc aaggccagac ggtgattgcc
     3421 gcacagcaag caccgaacat tctgacgctg gcagatatga gcactatgct ggtaaaagcg
     3481 caggtttctg aagcggatgt aatccacctg aagccggggc aaaaagcctg gtttacggtg
     3541 cttggcgatc cactgacgcg ctacgagggg caaatcaagg atgtgctacc gacgccggaa
     3601 aaggtaaacg acgctatttt ctattacgcc cgttttgaag tacccaaccc caatggtttg
     3661 ctgcggctgg atatgaccgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgttg
     3721 acgatccctc tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg
     3781 ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat
     3841 gttgagattg tcaaagggct ggaagcgggc gatgaagtgg tgattggtga ggccaaacca
     3901 ggagctgcac aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg
     3961 gtgatgagca ggttgaggtg ctgaagggca tcagcctcaa tatttatgcg ggtgagatgg
     4021 tcgcgattgt tggcgcttcg ggttccggta aatcgaccct gatgaatatt ctcggctgcc
     4081 tggataaggc caccagcggc acctatcgcg tagccggtca ggatattgcc acgctggacg
     4141 ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt tattttccag cgttaccatt
     4201 tgcttccgca tttaaccgtc gagcagaacg ttgaagtacc cgccgtctat gccggtcttg
     4261 agcggaaaca gcgactgctt cgtgcccagg agttgctgca acggctgggg ctggaagacc
     4321 gtacagagta ttatccggca cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc
     4381 gggcattgat gaacggcggt caggtaattc ttgccgatga accgaccggc gcactggaca
     4441 gccattctgg cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg
     4501 tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc
     4561 gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt gctggcggga
     4621 cggagcctgt tgtcaataca gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg
     4681 cgctgacgat ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc
     4741 tggggattat tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca
     4801 aacaaatggt gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg
     4861 ggaaagattt tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa
     4921 tcgccatcca aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg caaaacctgc
     4981 gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt
     5041 ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt taatcaggag cagctgaacg
     5101 gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg ccagcttttc ccccataaag
     5161 cagatgtggt tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg
     5221 cggaagaaaa acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca
     5281 gcacgatgtc cgggcgagtt atgggccagt cgtggcttaa ctccattact gtcagggtga
     5341 aagaaggatt tgacagcgcc gaggcggaac agcaactcac gcgtttactt tcactgcgcc
     5401 acggtaagaa ggatttcttt acctggaaca tggacggcgt cttgaaaact gttgaaaaga
     5461 ccacacgtac tttacaactg tttctgacgc tggtggcggt gatttcgctg gtggtgggcg
     5521 gtattggtgt aatgaatatt atgctggtgt cagtgaccga gcggacgcgg gaaattggca
     5581 ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca acagttcctg atcgaagccg
     5641 tactggtttg cctggtcggt ggcgcgttgg gaataacact gtcactgtta attgctttca
     5701 ccttgcagct tttcttaccc ggctgggaga ttggtttttc accgttggcg ctgctgctgg
     5761 cgtttctctg ctcgacggcc accgggattt tatttggctg gttacccgca cgaaatgcgg
     5821 cacgactgga tccagtagat gctctggcac gggagtaatt tttgagataa aaatgccagc
     5881 cgatcgggct ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact
     5941 gccgcttcta cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg
     6001 acatcaaact gaacggattg tccagctttt agcgttctgt aaccatccat ctgaatggtg
     6061 gaataatgag cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaccctttg
     6121 gcattgttga accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta
     6181 tacaagtaag atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc
     6241 tagaaataca atttagagaa ttagggcgag ccgtcaagca tttgacaggg gccagggggc
     6301 aggtatgaat caaaaatttg aagcagttaa cgctattgac aggaatgtga cagatgtcgc
     6361 tgatgccaac gatagatgat agttatctat catgtggagt agattggtca ggcaaataag
     6421 ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc gaagatgata actgacaatg
     6481 ggtaaaacga acgactggct ggactttgat caactggcgg aagaaaaagt tcgcgacgcg
     6541 ctaaaaccgc catctatgta taaagtgata ttagtcaatg atgattacac tccgatggag
     6601 tttgttattg acgtgttaca aaaattcttt tcttatgatg tagaacgtgc aacgcaattg
     6661 atgctcgctg ttcactacca ggggaaggcc atttgcggag tctttaccgc cgaggttgca
     6721 gaaaccaaag tggcgatggt gaacaagtac gcgagggaga atgagcatcc attgctgtgt
     6781 acgctagaaa aagcctgaat gcaggcataa aaattggggg aggtgcctat gctcaatcaa
     6841 gaactggaac tcagtttaaa tatggctttc gccagagcgc gcgagcaccg tcatgagttt
     6901 atgaccgtcg agcacttgtt actggcgctg ctcagtaacc catctgcccg ggaggcgctg
     6961 gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa
     7021 accacacccg ttctgcctgc cagtgaagag gagcgcgaca cacagccgac gctgagtttt
     7081 cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct ccggtcgcaa tgaggttacc
     7141 ggtgcaaacg ttctggtcgc tatcttcagc gaacaggagt cgcaggcggc atatctgttg
     7201 cgtaaacatg aagtcagccg tctcgatgtg gtgaacttta tctctcatgg cacgcgtaaa
     7261 gacgagccga cacagtcttc tgatcctggc agccagccaa acagcgaaga acaagctggt
     7321 ggggaggaac gtatggagaa tttcacgacg aacctgaacc agcttgcgcg tgtgggcgga
     7381 atcgacccac taattggtcg tgagaaagag ctggagcgtg ctattcaggt tctctgccgt
     7441 cgccgtaaaa acaacccgct gctggtgggg gaatctggtg tcggtaaaac cgcgattgca
     7501 gaaggtcttg cctggcgaat tgttcagggt gatgtgccgg aagtgatggc tgactgtacg
     7561 atttactctc tcgatatcgg ttctctgtta gcgggcacaa aatatcgcgg cgactttgaa
     7621 aaacgtttta aagcgttgct caagcagctg gagcaggaca ctaacagcat cctgtttatt
     7681 gatgagatcc acaccattat cggtgcgggt gcagcgtctg gtggtcaggt cgatgcggct
     7741 aacctaatca aaccgttgct ctccagcggt aaaattcgcg taattggttc gacaacctat
     7801 caggagttca gcaacatttt cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt
     7861 gatattactg aaccgtcgat cgaagaaact gttcaaatca tcaatggcct gaaaccgaag
     7921 tatgaagcgc accacgacgt gcgttatacc gcaaaagcgg tgcgtgcggc ggtagagctg
     7981 gcggtgaaat acattaacga tcgtcatctg ccggataaag ccattgatgt tatcgacgaa
     8041 gcaggcgctc gcgcacgtct gatgccggta agcaaacgca agaaaaccgt taatgtggcg
     8101 gatattgagt ccgtggtggc ccgtattgca cgcattccag agaagagtgt ttctcagagt
     8161 gaccgcgata ccctgaaaaa actcagcgat cgcctgaaaa tgctggtctt cggccaggat
     8221 aaagccattg aggcgctgac tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa
     8281 cataaaccgg tcggttcgtt cctgtttgcc ggtcctaccg gggtcgggaa aacagaggtg
     8341 acggtacagc tttcgaaagc gttgggcatt gagcttctgc gctttgatat gtccgagtat
     8401 atggaacgcc ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat
     8461 cagggcggtt tgctgaccga tgcggtaatc aagcatccac atgcggtgct gctgctggac
     8521 gaaatcgaga aagcgcatcc ggacgtgttc aatattctgt tgcaggtgat ggacaacggt
     8581 acgctgaccg ataacaacgg acgcaaagcg gacttccgta acgtggtgct ggtgatgacc
     8641 actaacgccg gggtacggga aactgagcgc aaatccattg gtcttatcca ccaggataac
     8701 agcaccgatg cgatggaaga gatcaagaag atctttacac cggaattccg taaccgtctc
     8761 gacaacatta tctggttcga tcatctgtca actgacgtga tccatcaggt ggtggataaa
     8821 ttcatcgtcg agttgcaggt tcagctggat cagaaaggtg tttctctgga agtgagccag
     8881 gaagcgcgta actggctggc cgagaaaggt tacgaccggg caatgggcgc acgtccgatg
     8941 gcgcgtgtca tccaggacaa cctgaaaaaa ccgctcgcca acgaactgct gtttggttcg
     9001 ctggtggacg gcggtcaggt gacggttgcg ctggataaag agaaaaatga gctgacttac
     9061 ggattccaga gtgcacaaaa gcacaaggcg gaagcagcgc attaatctga ttgtcaggta
     9121 ggttggtgaa gtccgtaatc tcgtcagggg ttacggactt tttatttatg gggtggaacg
     9181 gtgaaaaccc tatttttgga ggtgaaggta agttgttgat aattagtgct gctggaaggt
     9241 aaggataaaa aagggtgctg caggagaatg ggatggtttt gctttattaa caacgggcta
     9301 aacgtgtagt atttgagttc actgccgtac aggcagctta gaaatcgacg gggtgcggta
     9361 aaacctttgc gaacgcgttc actgccgtac aggcagctta gaaattcaca ggtaacatac
     9421 tccacccgcc caccatgttc actgccgtac agacagataa aatgcgaaaa aaaagctcgc
     9481 actttcgtac gagctcttct ttaaatatgg cggtgagggg gggattcgaa cccccgatac
     9541 gttgccgtat acacactttc caggcgtgct ccttcagcca ctcggacacc tcaccaaatt
     9601 gttttgctac caaacctcat gggtggcaac ggggcgctac tatagggagt tggagtaaaa
     9661 cggtcaagaa gaattttaat gataattatt gtttgctcat actgtaaaca acttgtgcag
     9721 tatatctaca tcgagacaag ttatggactt atacttccaa agtacttcat acatatcaca
     9781 aaataaaaag gccggttaaa ccgacctttt actcgttctt tctcttcgcc catcaggcgg
     9841 taaaacaatc agcgactacg gaagacaatg cggcctttgc tcaggtcgta cggggtcagt
     9901 tcaacagtca ctttgtcgcc cgtcaggatg cggatgtagt ttttgcgcat tttaccggag
     9961 atgtgtgcag taaccacgtg accgttttct aactctacgc ggaacatggt attaggcaac
    10021 gtttcaagaa cggtaccttg catttcaata ttgtcttctt tggccatcta atcctctggg
    10081 gtatcactac cgtaatttga accggcaaga taatgccgaa gttctgtaaa taagtaaaga
    10141 tttgcgcgct aaatcgcaac aaacaggttc ggcacattac tccgaaaaca cacggctaag
    10201 ccgcaccaaa agcgcaacgt ataagggagc ggtgagataa acgatgggcg ttacctgacg
    10261 cgaaaaattc cttatcggca gcggggtaat gagcgtaacc aactctgcga ccgcaattat
    10321 aacactctgg ggagaaatgt gccgaaaaca ttcattcttg tggtgaaaac aagcaccgtg
    10381 gtacccagaa attattcggc aatcgtccga ggcgcatttg attgagataa ttaaggtaat
    10441 cccggcgggg aatttcgcag gcaccaagcg atgctgtgtg atcgttaagg acctggcagt
    10501 cgataagctt accgccatga ccgataaatt cctcacagaa taccagaagc gccgttttag
    10561 acgcattttc catccggctg aacatggact cgccacaaaa tagcgttccc tgggccacgc
    10621 cgtacatacc gccgacaagc tcatcttcac gccagacttc aatggagtgg gcatgaccga
    10681 gttcgtgaag gcgatggtag gcttcgacca cgccacgcgt gatccaggtt ccttcttcgc
    10741 gatcgctggc acagccttca atgacctgac caaaagcgta attcatcgtg acacgatagg
    10801 gcgagcgttt atgaaatcgc ttcatactac ggctgatatg cagtgattct ggccatagca
    10861 ccgcgcgggg atcgggcgac caccagagga tggggtcgtc tggagaaaac cacggaaaaa
    10921 taccacgctg gtaagccatt aacaggcgcg cagggctaag atcgccccca agtgccagca
    10981 ggccgttagg ctcacgtaat gcgccttccg gggaagggaa ggctattgaa tggcgagaaa
    11041 gctgaaccaa gcgcatgacc gcaaaactcc acgcaagtcg gatcgttcaa taatagctta
    11101 caaaccctgc ttgaactggt aataacgccc ctgtctggca agcagttctg cgtgagtacc
    11161 ttgctcaata atttgcccgt tgtccatcac tattatttgt tggaaacgag agagtccgcg
    11221 aagtcgatgg gtgaccatta acaccgtttt ctcacgcatc atttttgcaa gcaattcaag
    11281 gatctggctt tcggttgtgg catctaagcc ttcggtaggt tcatccagca acaccagtgg
    11341 cgcatcatgt aacagcgtac gggcgatagc cagacggcgc agttcaccac cggagagctg
    11401 gcgtccgcct tcacctaacc aactgttgag acctgcatcc tcgagcagct tctccagacc
    11461 aacgcgacgc aagatctccg acagagcctc atcactactg ccaggcgagg cgagtaacag
    11521 attatcacgc agtgtggcgc taaacagatg cactcgctga ggaacaacgc tgatggtctg
    11581 tcgtagagcc gcttcattca ggctggctat ggggctatcg ttaagcaaaa tctcgccctg
    11641 ttgcgggtcc catgcgcggg tcagcagttg taacagtgtt gatttgccgc atccggttcg
    11701 cccgagaatc gctatatgtt ccccggcgtt tacctgaaga gaaatccctt taagtgcctg
    11761 ttgggattgc tccggataag tgaactgaac atcccgtaat gtcagcgaaa cgcgatcggc
    11821 aacacgagtt tgggtatcag ggaaggtgac ctccggtttt tgatccgtta agtcagagat
    11881 acgtacggca gaggcaatga cttgccccag atgctgaaat gcacccgtta ctggtgccag
    11941 tgcttcaaac gcggctaacg cacaaaagac aaacagggca attaatgcgc cgggttgagc
    12001 attgccgcca acgccgccag acgccatcca cagcatcagg atcaccgcta acgcgccaat
    12061 gagcagcatt atcgcttgcg acaatgcggt cagttcagat tgacggcgtt gcgcttccag
    12121 ccattgaatt tctgtattct ctagttgcgt gcgataacga tcgctggcac caaaaatggt
    12181 cagctcagct tgcccttgca gccaggccgt cagttgttgg cgatactgtc cgcgaagatg
    12241 agtcagattt tgcccggtgc tttttcccgc acgataaaac agcggtggca tcaggaaaag
    12301 cgtcagtaac ataacgctgc ccagcgtaaa ggcgagggtg aaatcaagga aacttaaccc
    12361 gattgtcacc accataatca ccacaaaagc gcccaccagc ggcgagataa cgcgcaggta
    12421 aagatgatcg agcgtatcaa catccgccac cacgcggttg agcaattcgc cctgacgata
    12481 gcgcgccagt ccggcagggg agaggggcaa caatttgctg aaggtgtaaa tgcgcagatg
    12541 ctgcaacacg cggaaagtcg cgtcgtgact taccagacgt tcaaaataac gcccggcagt
    12601 acgggtgatt gcggcaccac gcacgcccgc agcgggtagc atatagttga agctgtacag
    12661 tccggcaacc cccgcaaccg ctgaggccga gaggaaccag ccggaaagtg tcaacagacc
    12721 gatactggcg agcagcgtca caattgccag cacaatacca agacttaaca tccatttatg
    12781 acgtttatac agtgccagat agggtagcaa agcgcgcatt taaatctcct cctgacgatg
    12841 ggccagtaat gtggcgaatg ggccaccagc cacacttaat tccgcgtaac gtccttgctc
    12901 aataatccgg ccatcctgca taacccaaat gacatcccag tcagcaagat cttctaactg
    12961 gtgggtgacc attaacgttg tctggcgcag agaggcggca ttcagcgcct ccattacgcg
    13021 ctgttcactg tgagcatcaa ggctggcagc gggttcatcc aacagtaata gcgaacaggg
    13081 atttagtaac gcacgggcca ccgccacgcg ctgcgcctgc cccacggaaa ggcgggcagc
    13141 ctggtcgcca acaggtgtat caacgccttg tggcaggagc ggtagaaact cgctgaccca
    13201 ggcgttatcc agcgctgctt gtaattcttg ttcgctggca tcaggtcgcg ccagtagtac
    13261 gttatcccgc aattttgctg ccggtaattg tgggttttgc ccaacccagg agagatgttt
    13321 acgccatgat tctggtgata aatcacgtaa ttctatcccg ttgattcgta atgatccctg
    13381 atatgagaga aaaccagaaa gcgcgttcag cagtgagctt ttacctgaac cgctgcggcc
    13441 aaccaacact gcacgttggc ctgctggcaa agtaaagttc agcggtccgg ccagcgtttt
    13501 accttccggc gacgtgataa acagatcctc ggcctcaatg gtcagcggat cggtcaatgc
    13561 taattccgcc tcaccgcgct gcggatgggc gagcggggtt tccataaacg ttttcagact
    13621 gtcagctgca ccaacagcct gggctttagc atgataaaac gtaccgagat cgcgtaatgg
    13681 ctggaaaaac tctggcgcaa ggatcagggc cagaaaacct gcagccagcg tcaccccagt
    13741 atcgtagtga ccaaaatcca gctcgccgag ataggaaaaa ccaaagtaga ccgccaccag
    13801 agcaattgac agcgaggtaa aaaattcgag aatgccggag gataaaaacg ccagtcgtag
    13861 cacttccatt gtccgttggc ggaaatcttc cgaagcagaa cgaatacttt caatttcagc
    13921 ttcaccacga ccaaaaatac gcaatgtttc catgccgcgc aggcgatcga ggaaatgccc
    13981 acttaagcga gcaagagcga gaaagttacg ccggttagca tcggcagccc ccattccaac
    14041 cagcgccata aacaacggaa ttagcggtgc tgtgcccagc agaatgagcg ccgcagccca
    14101 gttagagggg aagatcgcca ccacaatcag caacggcacc gacactgcca gcgccatttg
    14161 cggcagatag cgtgcatagt aatcatgcat atcgtcaatt tgctcgagta ccagcgtcgc
    14221 ccagctcccc gcaggtttac cctgaatcca cgctggccct gcttgttgca gacggtcgag
    14281 aacctgacgg cggatggcaa agcggatatg ctgcccggcg tgataaccca cccgttcgcg
    14341 taaccagacc acccatgcgc gcaatacaaa ggtcagaacc agtaacgtaa agggaagcag
    14401 cagggcttca cggggaatat tctccataat catatgttgc agaattcgcg ccatgaacca
    14461 ggcctgggca atgatcaata tgccgcttac aaagcccagc agacgagaaa tattcagcca
    14521 acgttgggag atgacgcttt gctgttttaa ccagcgggtt aactcttttt gacgagattt
    14581 attcattgca cgcttagcag gtgagttatc agaattattt gcagagcaat gttacaacgg
    14641 ggaaaaaata aaggcgaccc atagtcgcaa ggtgtcgcct tctttacttt tgttactgat
    14701 ttgtaaaatt attttgcgtc agctaaacca tcgaggtagc gttccgcatc aagtgctgcc
    14761 atgcagccag taccggccga agtaatggcc tggcgataaa tgtgatccat cacgtcgcct
    14821 gcggcaaaga cgccaggaat gctggtctgg gtggcattac catgaatacc cgactgtact
    14881 ttgatgtagc cgttttccag ttccagctgc ccttcgaaaa tcgcagtatt cgggctgtga
    14941 ccgatagcaa caaacagacc ggcaacgtcg agtgactcga tgttatcgct gttttgcgta
    15001 tcgcgcagac gaacgccagt gacacccatt tgatcgccgg tcacttcttc cagcgtacgg
    15061 ttggtgtgca gaatgatgtt gccgttctcc actttatcca tcagacgctt aataaggatt
    15121 ttttccgcgc ggaaaccgtc acggcggtga atcagatgca cttccgaagc gatgttagac
    15181 agatacagcg cctcttcaac cgcggtattg ccgccgccga tgaccgcaac tttctggttg
    15241 cgatagaaga aaccgtcgca ggttgcacaa gcagaaaccc cacggccttt aaaggcttct
    15301 tcagagggca ggccgagata gcgtgcagaa gctccggtgg caataatcag cgcgtcgcaa
    15361 gtgtattcgc cgttatcgcc attcagacgg aacggacggt tttgcagatc caccttgttg
    15421 atatgatcaa aaatgatctc agtttcaaac ttggtggcat gttcgtgcat gcgcgccatt
    15481 aataacggac cggtcagatc gtttggatca ccaggccagt tttccacttc cgtggtggtg
    15541 gtcagttggc cgcctttttc catgccggta atcagcacag gttgcaggtt ggcgcgcgcc
    15601 gcgtagacag cagcggtgta tcccgccggg cctgaaccca ggataagcag tttactgtgt
    15661 ttggtcgtgc ccatgagatc cccatagttg ttggcagaca atgggcagga ttgtagggaa
    15721 tttacagacg taaaaaaaga gtatgacgat tttgttaaca atttgtgcaa tcggcagcat
    15781 cgataagcag gtcaaattct cccgtcatta tcacctctgc tacttaaatt tcccgcttta
    15841 taagccgatt aaatgatgaa taaacgcccc tgttaatgaa tatctggcat gttgtactaa
    15901 aaatcgatgt tttgctttga caatcccctg gtgttttgcg aaaacattcg aggaagaaaa
    15961 aaacagtatt cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg
    16021 aaatctacgt atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac
    16081 ggtcttctca ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc
    16141 gtgacgggta gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat
    16201 acagagagac aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta
    16261 tcgatcgtaa cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt
    16321 ctaaacgtgt gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac
    16381 aagggtttat tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc
    16441 tggtattcgt tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata
    16501 ccgctgtaca aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact
    16561 acctgttgaa aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc
    16621 tgctgcgtct gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc
    16681 agagtaatcg tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt
    16741 ttgattacac tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc
    16801 cgttttagca tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata
    16861 cactgaagac aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc
    16921 attgctgatc cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa
    16981 cccttcggac cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg
    17041 gatgcccggt gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac
    17101 cattcccgtc attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga
    17161 atacattgat tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac
    17221 ctcctgtggt ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt
    17281 cattggcagc ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat
    17341 tgcgctgctc tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac
    17401 cattgctgaa aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac
    17461 ccgtcgcgat gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga
    17521 tgaaaatcac ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc
    17581 gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc
    17641 gttgttctcc ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt
    17701 ggctgccgac ccggacgacg tcctattttc gggcaatcgt gcaacgcagc cagaatatga
    17761 cgaatacgat ccattattaa acagtgcgcc aattaccgaa cctgtcgctg tagcagctgc
    17821 tgctaccacg gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc
    17881 tgttgcctct gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg
    17941 tccacaaacg ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca
    18001 atatgcgcag cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca
    18061 gccgtattat gcacctgcag ctgaacaacc tgcgcaacag ccgtattatg cccctgcgcc
    18121 agaacaaccg gtggcaggta acgcctggca agccgaagag cagcaatcca cttttgctcc
    18181 acagtctaca taccagactg agcaaactta tcagcagcca gccgctcagg agccgttgta
    18241 ccaacagccg caacccgttg aacagcagcc tgttgtggag cctgaacccg ttgtagaaga
    18301 gacaaaaccc gcgcgtccgc cgctttacta ttttgaagaa gtggaagaga agcgagcccg
    18361 tgaacgtgaa caacttgcgg cctggtatca accgattcca gaaccggtta aagaaccaga
    18421 accgatcaaa tcttcgctga aagcaccttc tgttgcagca gtacctccag tagaagccgc
    18481 tgccgctgtt tctccgctgg catctggcgt gaaaaaagcg acactggcga cgggggctgc
    18541 cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc ggtggaccgc gtccgcaggt
    18601 caaagagggg attggcccgc agttgccacg accgaaacgt atccgcgtgc caactcgtcg
    18661 tgaattggcg tcttacggta ttaagctgcc ctcacagcgt gcggcggaag aaaaagcccg
    18721 tgaagcccag cgcaatcagt atgattctgg cgatcagtat aacgatgatg aaatcgatgc
    18781 gatgcagcag gatgaactgg cacgtcagtt cgcccagaca cagcagcaac gctatggcga
    18841 acagtatcaa catgatgtgc ccgtaaacgc agaagatgca gatgctgcgg cagaggctga
    18901 actggctcgt cagtttgcgc aaactcaaca acaacgttat tccggcgaac aaccggctgg
    18961 ggcgaatccg ttctcgctgg atgattttga attttcgcca atgaaagcgt tgctggatga
    19021 tggtccacac gaaccgctgt ttacgccaat tgttgaacct gtacagcagc cgcaacaacc
    19081 ggttgcacca cagcagcagt atcagcagcc acaacagccg gttgcgccac agcagcagta
    19141 tcagcagcca caacagcagg ttgcgccgca gccacaatat cagcagccgc aacaaccggt
    19201 tgcgccacag cagcaatatc agcagccgca acaaccagtt gcgccgcagc cgcagtatca
    19261 acagccgcaa caaccggttg caccgcagcc gcagtatcag cagccacaac agccagttgc
    19321 gccacagccg caggataccc tgcttcatcc gctgttgatg cgtaatggcg acagccgtcc
    19381 gttgcacaaa ccgacgacgc cactgccttc tctggatttg ctgacaccgc cgccgagcga
    19441 agtggagccg gtagatacct ttgcgcttga acaaatggcg cgtctggtgg aagcgcgtct
    19501 ggctgatttc cgtattaaag ccgatgtcgt taattactct ccggggccag ttatcactcg
    19561 ctttgaactt aacctggctc ctggcgtaaa agcggcgcgc atttctaact tgtcacggga
    19621 ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt gaagttattc ctggcaaacc
    19681 ctatgtaggt ctggagttac caaataaaaa acgacaaacc gtttatctgc gcgaagtttt
    19741 ggataacgcc aaattccgcg ataatccgtc gccattaacc gtggtgctgg gtaaagatat
    19801 cgccggtgag ccggtggttg ccgatctggc gaaaatgccg cacttgttgg ttgcggggac
    19861 taccggttcc ggtaaatctg tcggtgtgaa cgcgatgatc ctgagcatgc tttataaagc
    19921 acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc tttcggttta
    19981 tgaaggcatt ccgcacctgt taacggaagt cgttactgat atgaaagatg ccgccaacgc
    20041 gctgcgctgg tgtgttaacg agatggagcg tcggtataaa ctgatgtctg cgctgggtgt
    20101 gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc gatcgcatga tgcgtccgat
    20161 tccagacccg tactggaagc cgggtgacag tatggatgcc cagcatccgg tgctgaaaaa
    20221 agaaccatac attgtcgtgt tggttgacga atttgccgac ctgatgatga cggtaggtaa
    20281 aaaagtggaa gagctgatag ctcgtctggc gcaaaaagcc cgtgccgcgg gtatccacct
    20341 cgtactggca actcagcgtc catcggttga tgttattact ggtctgatta aagcgaatat
    20401 tccgacccgt atcgccttta ccgtatccag taagattgac tcacgtacca ttcttgatca
    20461 ggctggcgcg gaatcactgc tgggtatggg ggatatgctc tactctgggc cgaactccac
    20521 gttgccggta cgtgtccatg gtgcttttgt tcgcgatcag gaagttcatg ccgtggtgca
    20581 ggactggaaa gcgcgtggtc gcccacagta tgttgatggc atcacctccg acagcgaaag
    20641 cgaaggtggt gcgggtggtt tcgatggcgc tgaagaactg gatccgttgt tcgatcaggc
    20701 ggtgcagttt gtcactgaaa aacgcaaagc gtcaatttct ggcgtacagc gtcagttccg
    20761 cattggttat aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg ggattgtcag
    20821 cgaacagggg cacaacggta atcgtgaagt gctggcccca ccgccgtttg actaattaat
    20881 gcattgccgg ataaggcgcg gtagcgtcgc atccggcact ctatcaactg aaaattcagt
    20941 attttcttct ttcctcaagc tgattattag cctggaatag agagtagagg gaactcccga
    21001 tcgggagtga cgtaatttga ggaataatga tgaaaaaaat tgctatcacc tgtgcattac
    21061 tctcaagctt agtagcaagc agcgtttggg ctgatgccgc aagcgatctg aaaagccgcc
    21121 tggataaagt cagcagcttc cacgccagct tcacacaaaa agtgactgac ggtagcggcg
    21181 cggcggtgca ggaaggtcag ggcgatctgt gggtgaaacg tccaaactta ttcaactggc
    21241 atatgacaca acctgatgaa agcattctgg tttctgacgg taaaacactg tggttctata
    21301 acccgttcgt tgagcaagct acggcaacct ggctgaaaga tgccaccggt aatacgccgt
    21361 ttatgctgat tgcccgcaac cagtccagcg actggcagca gtacaatatc aaacagaatg
    21421 gcgatgactt tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag ttcaccatta
    21481 acgtgggacg tgatggcaca atccatcagt ttagcgcggt ggagcaggac gatcagcgca
    21541 gcagttatca gctgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa tttaccttca
    21601 ccccgccgca aggcgtcacg gtagatgatc aacgtaagta gaggcacctg agtgagcaat
    21661 ctgtcgctcg atttttcgga taatactttt caacctctgg ccgcgcgtat gcggccagaa
    21721 aatttagcac agtatatcgg tcagcaacat ttgctggctg cggggaagcc gttgccgcgc
    21781 gctatcgaag ccgggcattt gcattctatg atcctctggg ggccaccggg taccggcaaa
    21841 acaaccctcg ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg tatttctgcc
    21901 gtcacctctg gcgtgaaaga gattcgcgag gcgatcgagc gcgcccggca aaaccgcaat
    21961 gcaggtcgcc gcactattct ttttgttgac gaagttcacc gtttcaacaa aagccagcag
    22021 gatgcatttc tgccacatat tgaagacggc accatcactt ttattggcgc aaccactgaa
    22081 aacccgtcgt ttgagcttaa ttcggcactg ctttcccgtg cccgtgttta tctgttgaaa
    22141 tccctgagta cagaggatat tgagcaagta ctaactcagg cgatggaaga caaaacccgt
    22201 ggctatggtg gtcaggatat tgttctgcca gatgatacac gacgtgccat tgcagaactg
    22261 gtgaatggcg acgcgcgccg ggcgttaaat acgctggaaa tgatggcgga tatggccgaa
    22321 gtctatgata gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat cgccggtgaa
    22381 cgtagcgccc gctttgataa caaaggcgat cgcttttacg atctaatttc cgcactgcat
    22441 aagtcggtac gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg aattattacc
    22501 gctggtggcg atccgttata tgtcgcgcgt cgctgtctgg cgattgcgtc tgaagacgtc
    22561 ggtaatgccg atccacgggc gatgcaggtg gcaattgcgg cctgggattg ctttactcgc
    22621 gttggcccgg cggaaggtga acgcgccatt gctcaggcga ttgtttacct ggcctgcgcg
    22681 ccaaaaagca acgctgtcta cactgcgttt aaagccgcgc tggccgatgc tcgcgaacgc
    22741 ccggattatg acgtgccggt tcatttgcgt aatgcgccga cgaaattaat gaaggaaatg
    22801 ggctacgggc aggaatatcg ttatgctcat gatgaagcaa acgcttatgc tgccggtgag
    22861 gtttacttcc cgccggaaat agcacaaaca cgctattatt tcccgacaaa caggggcctt
    22921 gaaggcaaga ttggtgaaaa gctcgcctgg ctggctgaac aggatcaaaa tagccccata
    22981 aaacgctacc gttaatgtta tcgttgcggt aatgttgtta ctgtatccct gtggtcgcag
    23041 gctgtggcca catctcccat ttaattcgat aagcacagga taagcatgct cgatcccaat
    23101 ctgctgcgta atgagccaga cgcagtcgct gaaaaactgg cacgccgggg ctttaagctg
    23161 gatgtagata agctgggcgc tcttgaagag cgtcgtaaag tattgcaggt caaaacggaa
    23221 aacctgcaag cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa agcgcgcggg
    23281 gaagatatcg agcctttacg tctggaagtg aacaaactgg gcgaagagct ggatgcagca
    23341 aaagccgagc tggatgcttt acaggctgaa attcgcgata tcgcgctgac catccctaac
    23401 ctgcctgcag atgaagtgcc ggtaggtaaa gacgaaaatg acaacgttga agtcagccgc
    23461 tggggtaccc cgcgtgagtt tgactttgaa gttcgtgacc acgtgacgct gggtgaaatg
    23521 cactctggcc tcgactttgc agctgcagtt aagctgactg gttcccgctt tgtggtaatg
    23581 aaagggcaga ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga tctgcatacc
    23641 gaacagcatg gctacagtga gaactatgtt ccgtacctgg ttaaccagga cacgctgtac
    23701 ggtacgggtc agctgccgaa atttgctggc gatctgttcc atactcgtcc gctggaagaa
    23761 gaagcagaca ccagtaacta tgcgctgatc ccaacggcag aagttccgct gactaacctg
    23821 gtgcgcggtg aaatcatcga tgaagatgat ctgccaatta agatgaccgc ccacacccca
    23881 tgcttccgtt ctgaagccgg ttcatatggt cgtgacaccc gtggtctgat ccgtatgcac
    23941 cagttcgaca aagttgaaat ggtgcagatc gtgcgcccag aagactcaat ggcggcgctg
    24001 gaagagatga ctggtcatgc agaaaaagtc ctgcagttgc tgggcctgcc gtaccgtaaa
    24061 atcatccttt gcactggcga catgggcttt ggcgcttgca aaacttacga cctggaagta
    24121 tggatcccgg cacagaacac ctaccgtgag atctcttcct gctccaacgt ttgggatttc
    24181 caggcacgtc gtatgcaggc acgttgccgc agcaagtcgg acaagaaaac ccgtctggtt
    24241 cataccctga acggttctgg tctggctgtt ggtcgtacgc tggttgcagt aatggaaaac
    24301 tatcagcagg ctgatggtcg tattgaagta ccagaagttc tgcgtccgta tatgaacgga
    24361 ctggaatata ttggctaata cccaattttt ctgaatctaa aaagcgcctt tagggcgctt
    24421 tttttgtctc cctttgatac cgaacaataa ttactcctca cttacacgta atactacttt
    24481 cgagtgaaaa tctacctatc tctttgattt tcaaattatt ctatgtatac aagcctatat
    24541 agcgagctgc tatagaaata attacacaat acggtttgtt actggaatca atcgtgatca
    24601 agcttgagtg agccattatg aaaacgaaaa tccctgatgc ggtattggct gctgaggaga
    24661 gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct ggcaatggcc agcagcgcat
    24721 taacattacc ttttagtcgg attgcgcacg ctgtcgatag cgccattcca acaaaatcag
    24781 acgaaaaggt tatctggagc gcctgtacag ttaactgtgg tagtcgctgc ccgctacgta
    24841 tgcacgtcgt ggacggtgaa atcaaatatg tcgaaacgac aataccggcg atgacaatta
    24901 cgacggccta cactaggttc gcgcctgcct gcgtgggcgt tccatgcgtc accgtgtcta
    24961 caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt
    25021 cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat
    25081 caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc tgggcggcac
    25141 catgacccgc tcctggccgc cgggaaatac cctggtcgcg cgcctgatga actgctgcgg
    25201 cggttatctt aaccattacg gtgactactc ttccgcgcaa attgcggaag gtttgaacca
    25261 tacctacggc ggctgggcag atggtaacag cccgtcggat atcgaaaaca gtaaattggt
    25321 agtgctgttt ggtaataacc caggcgaaac gcgaatgagt ggcggtgggg tgacttacta
    25381 tcttgaacag gcacgccaga aatctaatgc tcgcatgatc atcatcgatc cgcgctatac
    25441 cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccaggaa cagatgccgc
    25501 actggttaac ggtctggcgt acgtcatgat cactaaaaac ctggtggatc aggcattcct
    25561 cgataaatat tgcgttggct atgacgagaa aaccctgccc gccagtgcgc cgaaaaatgg
    25621 tcattataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa caccggaatg
    25681 ggcctcgcaa atcaccggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg
    25741 cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga
    25801 aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaatgg
    25861 agggaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgacctt
    25921 ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg
    25981 cccggaaatg acggcgctgc gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat
    26041 caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg
    26101 tacccatgaa atcctccagg atgataagaa gtgcgagctg attgtggtta tcgactgcca
    26161 catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca
    26221 gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca
    26281 ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa
    26341 acgtcttggc gttgagcaac agtttactga aggccgtacc caggaagagt ggatgcggca
    26401 tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa
    26461 gcaggggatc tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg
    26521 tgaagatccg caggcaaacc cactgactac gccatcgggc aaaattgaga tttattcgca
    26581 ggcgctggct gacattgccg ctacctggga attgccagaa ggcgatgtga tcgatccact
    26641 gccgatctac acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct
    26701 gcagcttaca ggtttccact ataaatctcg cgttcactca acttacggca acgttgatgt
    26761 gctgaaagcg gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg
    26821 tatccacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc
    26881 gaaagtgacg ccacgaatga tgccgggcgt ggtcgcactg ggtgaaggcg cctggtatga
    26941 cccggatgca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca ctcaacgtcc
    27001 gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta
    27061 aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt
    27121 gcaaaacctg cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagtttcc
    27181 gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga
    27241 acgtgtttgc ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta
    27301 aagtctgccc gagcggtgcg atgcataaac gcgaagatgg ttttgttgtg gtcgatgaag
    27361 atgtatgcat tggctgccgt tactgtcata tggcttgccc gtatggcgca ccgcaatata
    27421 acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggta
    27481 aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct ggatttcggc cctatcgacg
    27541 agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta
    27601 ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg gataccactg
    27661 gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg
    27721 atgatcttca cggtcttcgg gcaatgtgtg gcgggtggtt ttatcgttct ggctttggcg
    27781 ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg catgtttggc
    27841 ttatgggtac tgatgggcat tggctttatc gcctctatgc tccatcttgg ttcaccgatg
    27901 cgcgccttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc
    27961 ggttcgatct tttttgctgt aggcggcatc ggctggttgc tggcaatgct gaaaaagctg
    28021 tcaccggcat tgcgtacgct atggctggtg gtgacgatgg ttcttggcgt cgtctttgtc
    28081 tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg
    28141 acgccgatgg gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg
    28201 agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag
    28261 caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcggat cgtgcttttg
    28321 gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg
    28381 ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc
    28441 tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt
    28501 tcggccccgg ttctctttta tttctgcttc gttaacgtgt cataactggt catcaaatta
    28561 cgataatcag gaatatggtt agagaacaac gtcgccagtc cttcaatatc attacgccag
    28621 tcacggtgca gctcgcaagc cacgccgaac catgtcatca attgcgcacc agcttgcgac
    28681 atgcgatccc acgccgaatg ccgggtaatt tcattaaagg tccctgatgc atcagtcacg
    28741 acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg
    28801 gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa
    28861 tcttcgttat cccaggcgtt aatattgccc gggcgagcaa tgtaaggtgc atcggggaat
    28921 tgtgctttca gttctagaac taatgggccg ttaggaccgg tttcaaaact ggtggtaaga
    28981 atagttggta agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac
    29041 ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctggtgatc aacaagcaaa
    29101 acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga
    29161 gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa gcatagacgg
    29221 gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt
    29281 gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta gcgttatgaa
    29341 tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca
    29401 atgtttttgg cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat
    29461 gtccacgtat acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc
    29521 gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg
    29581 gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac
    29641 agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctgtcct ccttcatttt
    29701 tgctgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt tggcgtggcg
    29761 ttttgtcgcg ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg cgctgatgtg
    29821 cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg tttattacgt
    29881 gggaacgttt ttaggccagt tactggtcag caaagtctca accgagctga tgagcgtatt
    29941 gccgtgggtt acaggtttga cattggcagg gatcttaccg ctgttgttta cgcgtgtcct
    30001 gaatcagcag gctgaaaacc atgattcgac gtcaattacg gcaatgctaa aactccgtca
    30061 ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gttctgggat ctctatatgg
    30121 cctgatgcct ctgtacctca atcacaaagg ggtgagcaat gccagtattg gtttctggat
    30181 ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc tggcggataa
    30241 gtttggtcga ctgctggttt tgcgtgttca ggtctttgtc gtcattctcg gcagtattgc
    30301 gatgcttagc caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac
    30361 gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc aactggtggc
    30421 gatgaaccaa gccttactgt tgagctatac cgtgggaagt ctgcttggcc cgtcatttag
    30481 cgctatgcta atgcagaatt tctccgataa tttatt
//
LOCUS       DAJTNP010000040        30296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTNP010000040 DAJTNP010000000
VERSION     DAJTNP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO160_002008"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO160_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5524140.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO160_002009"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO160_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5524141.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO160_002010"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO160_002010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5524142.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO160_002011"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO160_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO160_002012"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO160_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5524143.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO160_002013"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO160_002013"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5524144.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO160_002014"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO160_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5524145.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OO160_002015"
     CDS             7490..7639
                     /locus_tag="OO160_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524146.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OO160_002016"
     CDS             7840..9423
                     /locus_tag="OO160_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5524147.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO160_002017"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO160_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5524148.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO160_002018"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO160_002018"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5524149.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO160_002019"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO160_002019"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5524150.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="OO160_002020"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="OO160_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="OO160_002021"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="OO160_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="OO160_002022"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="OO160_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="OO160_002023"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="OO160_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5524151.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="OO160_002024"
     CDS             20753..22168
                     /locus_tag="OO160_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524152.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="OO160_002025"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="OO160_002025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5524153.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="OO160_002026"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="OO160_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5524154.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="OO160_002027"
     CDS             24478..24810
                     /locus_tag="OO160_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5524155.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="OO160_002028"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="OO160_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5524156.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /locus_tag="OO160_002029"
     CDS             complement(26660..26977)
                     /locus_tag="OO160_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524157.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27383..28282
                     /gene="yegS"
                     /locus_tag="OO160_002030"
     CDS             27383..28282
                     /gene="yegS"
                     /locus_tag="OO160_002030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5524158.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28364..29137)
                     /locus_tag="OO160_002031"
     CDS             complement(28364..29137)
                     /locus_tag="OO160_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5524159.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="OO160_002032"
     CDS             complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="OO160_002032"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5524160.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8047 c         7829 g         7408 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 caaaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctattcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    27061 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
    27121 tcagggtaac tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa
    27181 tattaggtat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
    27241 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
    27301 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
    27361 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
    27421 taaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
    27481 gacgatccat gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc
    27541 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
    27601 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
    27661 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
    27721 gaaactggca attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac
    27781 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
    27841 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
    27901 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
    27961 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa
    28021 cgcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
    28081 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
    28141 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
    28201 gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
    28261 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat
    28321 gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt
    28381 ttacccgtgt cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa
    28441 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg
    28501 aatcgagaac ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac
    28561 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
    28621 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
    28681 tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    28741 acgctgccgg gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg
    28801 cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt
    28861 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    28921 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    28981 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    29041 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    29101 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    29161 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    29221 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac
    29281 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    29341 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    29401 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    29461 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    29521 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    29581 cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    29641 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    29701 agctaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    29761 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    29821 aatgaaaagc atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca
    29881 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    29941 catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac
    30001 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta
    30061 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    30121 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    30181 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg
    30241 caacgcgcac gataccatca gtatcattca ctactgattt cataaaaact cctgat
//
LOCUS       DAJTNP010000041        30213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTNP010000041 DAJTNP010000000
VERSION     DAJTNP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..219
                     /gene="tuf"
                     /locus_tag="OO160_002033"
     CDS             <1..219
                     /gene="tuf"
                     /locus_tag="OO160_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5524161.1"
                     /translation="FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            449..832
                     /gene="secE"
                     /locus_tag="OO160_002034"
     CDS             449..832
                     /gene="secE"
                     /locus_tag="OO160_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5524162.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            834..1379
                     /gene="nusG"
                     /locus_tag="OO160_002035"
     CDS             834..1379
                     /gene="nusG"
                     /locus_tag="OO160_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5524163.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1538..1966
                     /gene="rplK"
                     /locus_tag="OO160_002036"
     CDS             1538..1966
                     /gene="rplK"
                     /locus_tag="OO160_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5524164.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1970..2674
                     /gene="rplA"
                     /locus_tag="OO160_002037"
     CDS             1970..2674
                     /gene="rplA"
                     /locus_tag="OO160_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5524165.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3087..3584
                     /gene="rplJ"
                     /locus_tag="OO160_002038"
     CDS             3087..3584
                     /gene="rplJ"
                     /locus_tag="OO160_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5524166.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3651..4016
                     /gene="rplL"
                     /locus_tag="OO160_002039"
     CDS             3651..4016
                     /gene="rplL"
                     /locus_tag="OO160_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5524167.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4336..8364
                     /gene="rpoB"
                     /locus_tag="OO160_002040"
     CDS             4336..8364
                     /gene="rpoB"
                     /locus_tag="OO160_002040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5524168.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8441..12664
                     /gene="rpoC"
                     /locus_tag="OO160_002041"
     CDS             8441..12664
                     /gene="rpoC"
                     /locus_tag="OO160_002041"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5524169.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12906..14039)
                     /gene="thiH"
                     /locus_tag="OO160_002042"
     CDS             complement(12906..14039)
                     /gene="thiH"
                     /locus_tag="OO160_002042"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5524170.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14036..14806)
                     /gene="thiG"
                     /locus_tag="OO160_002043"
     CDS             complement(14036..14806)
                     /gene="thiG"
                     /locus_tag="OO160_002043"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5524171.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14808..15008)
                     /gene="thiS"
                     /locus_tag="OO160_002044"
     CDS             complement(14808..15008)
                     /gene="thiS"
                     /locus_tag="OO160_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5524172.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14992..15747)
                     /gene="thiF"
                     /locus_tag="OO160_002045"
     CDS             complement(14992..15747)
                     /gene="thiF"
                     /locus_tag="OO160_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5524173.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15740..16375)
                     /gene="thiE"
                     /locus_tag="OO160_002046"
     CDS             complement(15740..16375)
                     /gene="thiE"
                     /locus_tag="OO160_002046"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5524174.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16375..18270)
                     /gene="thiC"
                     /locus_tag="OO160_002047"
     CDS             complement(16375..18270)
                     /gene="thiC"
                     /locus_tag="OO160_002047"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5524175.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18351..18451)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18504..18980)
                     /gene="rsd"
                     /locus_tag="OO160_002048"
     CDS             complement(18504..18980)
                     /gene="rsd"
                     /locus_tag="OO160_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5524176.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19075..19848
                     /gene="nudC"
                     /locus_tag="OO160_002049"
     CDS             19075..19848
                     /gene="nudC"
                     /locus_tag="OO160_002049"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5524177.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19888..20952
                     /gene="hemE"
                     /locus_tag="OO160_002050"
     CDS             19888..20952
                     /gene="hemE"
                     /locus_tag="OO160_002050"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5524178.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21109..21780
                     /gene="nfi"
                     /locus_tag="OO160_002051"
     CDS             21109..21780
                     /gene="nfi"
                     /locus_tag="OO160_002051"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5524179.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21823..22413
                     /locus_tag="OO160_002052"
     CDS             21823..22413
                     /locus_tag="OO160_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5524180.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22600..22872
                     /gene="hupA"
                     /locus_tag="OO160_002053"
     CDS             22600..22872
                     /gene="hupA"
                     /locus_tag="OO160_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5524181.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22885..23580
                     /locus_tag="OO160_002054"
     CDS             22885..23580
                     /locus_tag="OO160_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5524182.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23582..24007)
                     /gene="zraP"
                     /locus_tag="OO160_002055"
     CDS             complement(23582..24007)
                     /gene="zraP"
                     /locus_tag="OO160_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5524183.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24245..25621
                     /gene="zraS"
                     /locus_tag="OO160_002056"
     CDS             24245..25621
                     /gene="zraS"
                     /locus_tag="OO160_002056"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5524184.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25618..26943
                     /gene="zraR"
                     /locus_tag="OO160_002057"
     CDS             25618..26943
                     /gene="zraR"
                     /locus_tag="OO160_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5524185.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26940..28229)
                     /gene="purD"
                     /locus_tag="OO160_002058"
     CDS             complement(26940..28229)
                     /gene="purD"
                     /locus_tag="OO160_002058"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5524186.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28241..29830)
                     /gene="purH"
                     /locus_tag="OO160_002059"
     CDS             complement(28241..29830)
                     /gene="purH"
                     /locus_tag="OO160_002059"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5524187.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7070 a         7879 c         8313 g         6951 t
ORIGIN      
        1 ttcttcaaag gctaccgtcc gcagttctac ttccgtacta ctgacgtgac tggtaccatc
       61 gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca acatcaaaat ggttgttacc
      121 ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg caatccgtga aggcggccgt
      181 accgttggcg cgggcgttgt agcaaaagtt ctgagctaat tgccgataac atttgacgca
      241 atgcgcacta aaagggcatc atttgatgcc ctttttgcac gctttcgtac cagaacctgg
      301 ctcatcagtg attttctttg tcataatcat tgctgagaca ggctctgttg agggcgtata
      361 atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg cgatcgcggg gtgaaaatgt
      421 ttgtagaaaa cttctgacag gttggtttat gagtgcgaat accgaagctc aaggaagcgg
      481 gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca ttgctcctgg cggcgattgt
      541 cggcaactac ctttatcgcg acattatgct gccgctgcgt gcgctggccg tagtaattct
      601 gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa ggtaaagcta ccattgcttt
      661 tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg ccgactcgcc aggaaacatt
      721 gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg tcactgatcc tgtggggact
      781 ggatggtatt ctggttcgcc tggtatcctt tatcactggc ctgaggttct gagatgtctg
      841 aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc cggttttgaa ggccgcgtag
      901 caacgtcgct gcgtgagcat atcaaattac acaacatgga agatttgttt ggtgaagtca
      961 tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca gcgtcgcaaa agcgaacgta
     1021 aattcttccc tggctacgtc ctcgttcaga tggtgatgaa cgacgcgagc tggcacttgg
     1081 tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac ttccgatcgt cctgcgccaa
     1141 tcagcgataa agaagtcgat gcgattatga accgcctgca gcaggttggt gataagccgc
     1201 gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt taatgatggt ccgttcgctg
     1261 acttcaacgg tgttgttgaa gaagtggatt acgagaaatc tcgtctgaaa gtgtctgttt
     1321 ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag ccaggttgaa aaagcctaac
     1381 ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg cgtgagattg gaatacaatt
     1441 tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat tttttatatc acggggagcc
     1501 tctcagaggc gttattaccc aacttgagga atttataatg gctaagaaag tacaagccta
     1561 tgtcaagctg caggttgcag ctggtatggc taacccgagt ccgccagtag gtccggctct
     1621 gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc
     1681 catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt tacgctgacc gttctttcac
     1741 tttcgttacc aagaccccgc cggcagcagt tctgctgaaa aaagcggcag gtatcaagtc
     1801 tggttccggt aagccgaaca aagacaaagt gggtaaaatt tcccgcgctc agctgcagga
     1861 aatcgcgcag accaaagctg ccgacatgac tggtgccgac attgaagcga tgactcgctc
     1921 catcgaaggt actgcacgtt ccatgggcct ggtagtggag gactaagaaa tggctaaact
     1981 gaccaagcgc atgcgtgtta tccgtgagaa agttgatgca accaaacagt acgacatcaa
     2041 cgaagctatc gcactgctga aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt
     2101 agctgttaac ctcggcatcg acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt
     2161 actgccgcac ggtactggcc gttccgttcg cgtagccgta tttacccaag gtgcaaacgc
     2221 tgaagctgct aaagctgcag gcgcagaact ggtaggtatg gaagatctgg ctgaccagat
     2281 caagaaaggc gaaatgaact ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt
     2341 tggccagctg ggccaggttc tgggtccgcg tggcctgatg ccaaacccga aagtgggtac
     2401 tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa gctggccagg ttcgttaccg
     2461 taacgacaaa aacggcatca tccacaccac catcggtaaa gtggactttg acgctgacaa
     2521 actgaaagaa aacctggaag ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc
     2581 gaaaggcgtg tacatcaaga aagttagcat ctccaccacc atgggtgcag gtgttgcagt
     2641 tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt
     2701 ctacaatctt acccccacgt ataatgctta atgcagacgt atatccgaga tattcgggtt
     2761 gtggcaaggc ggcaactgag tgagtcgcca agagcatagc taactatgtg actggtgcga
     2821 atgaaggaag ccaacgccgc cacaagttga atagcgacgg atagaaaaga tttgttcgtt
     2881 ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc
     2941 ccctgcgtag acggtgacag aacgctaaga ttattctttt atattctggc ttgtttctgc
     3001 tcaccgtaat taagacgctc tctccgtttg gaggagtgaa gtgagttcca gagattttct
     3061 ctggcaaaca tccaggagca aagctaatgg ctttaaatct tcaagacaaa caagcgattg
     3121 ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg
     3181 gcgtaactgt agataaaatg actgaactgc gtaaagcagg tcgcgaagct ggcgtataca
     3241 tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc
     3301 tgaaagacgc gtttgttggt ccgaccctga ttgcatactc tatggaacac ccgggcgctg
     3361 ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg
     3421 cctttgaagg tgagctgatc ccggcgtctc agatcgaccg cctggcaact ctgccgacct
     3481 acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg
     3541 ttcgtactct ggctgctgta cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt
     3601 aacgcattcg cttacgtata aacttattct gatattcagg aacaatttaa atgtctatca
     3661 ctaaagatca aatcattgaa gcagttgcag ctatgtctgt aatggacgtt gtagaactga
     3721 tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg
     3781 gcccggttga agctgctgaa gaaaaaactg aattcgacgt aattctgaaa gctgctggcg
     3841 ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag
     3901 ctaaagacct ggtagaatct gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg
     3961 cagaagcact gaaaaaagct ctggaagaag ctggcgctga agttgaagtt aaataagcca
     4021 acccttccgg ttgcagcctg agaaatcagg ctgatggctg gtgacttttt agtcaccagc
     4081 ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt tgactactgc tgtgcctttc
     4141 aatgcttgtt tctatcgacg acttaatata ctgcgacagg acgtccgttc tgtgtaaatc
     4201 gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg gaaagtgttc cattttccgg
     4261 tcaacaaaat agtgttgcac aaactgtccg ctcaatggac agatgggtcg acttgtcagc
     4321 gagctgagga accctatggt ttactcctat accgagaaaa aacgtattcg taaggatttt
     4381 ggtaaacgtc cacaagttct ggatgtacct tatctccttt ctatccagct tgactcgttt
     4441 cagaaattta tcgagcaaga tcctgaaggg cagtatggtc tggaagctgc tttccgttcc
     4501 gtattcccga ttcagagcta cagcggtaat tccgagctgc aatacgtcag ctaccgcctt
     4561 ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg
     4621 ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa
     4681 gacattaaag aacaagaagt ctacatgggc gaaattccgc tcatgacaga caacggtacc
     4741 tttgttatca acggtactga gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc
     4801 ttctttgact ccgacaaagg taaaacccac tcttcgggta aagtgctgta taacgcgcgc
     4861 atcatccctt accgtggttc ctggctggac ttcgaattcg atccgaagga caacctgttc
     4921 gtacgtatcg accgtcgccg taaactgcct gcgaccatca ttctgcgcgc cctgaactac
     4981 accacagagc agatcctcga cctgttcttt gaaaaagtta tctttgaaat ccgtgataac
     5041 aagctgcaga tggaactggt gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc
     5101 gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc
     5161 cagctggaaa aagacgacgt caaactgatc gaagtcccgg ttgagtacat cgcaggtaaa
     5221 gtggttgcta aagactatat tgatgagtct accggcgagc tgatctgcgc agcgaacatg
     5281 gagctgagcc tggatctgct ggctaagctg agccagtctg gtcacaagcg tatcgaaacg
     5341 ctgttcacca acgatctgga tcacggccca tatatctctg aaaccttacg tgtcgaccca
     5401 actaacgacc gtctgagcgc actggtagaa atctaccgca tgatgcgccc tggcgagccg
     5461 ccgactcgtg aagcagctga aagcctgttc gagaacctgt tcttctccga agaccgttat
     5521 gacttgtctg cggttggtcg tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa
     5581 ggttccggta ttctgagcaa agacgacatc attgatgtta tgaaaaagct catcgatatc
     5641 cgtaacggta aaggcgaagt cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc
     5701 gttggcgaaa tggcggaaaa ccagttccgc gttggcctgg tacgtgtaga gcgtgcggtg
     5761 aaagagcgtc tgtctctggg cgatctggat accctgatgc ctcaggatat gatcaacgcc
     5821 aagccgattt ccgcagcagt gaaagagttc ttcggttcca gccagctgtc tcagtttatg
     5881 gaccagaaca acccgctgtc tgagattacg cacaaacgtc gtatctccgc actcggccca
     5941 ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag acgtacaccc gactcactac
     6001 ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca tcggtctgat caactctctg
     6061 tccgtgtacg cacagactaa cgaatacggc ttccttgaga ctccgtatcg taaagtgacc
     6121 gacggtgttg taactgacga aattcactac ctgtctgcta tcgaagaagg caactacgtt
     6181 atcgcccagg cgaactccaa cctggatgaa gaaggccact tcgtagaaga cctggtaact
     6241 tgccgtagca aaggcgaatc cagcttgttc agccgcgacc aggttgacta catggacgta
     6301 tccacccagc aggtggtatc cgtcggtgcg tccctgatcc cgttcctgga acacgatgac
     6361 gccaaccgtg cattgatggg tgcgaatatg caacgtcagg ccgttccgac tctgcgcgct
     6421 gataagccgc tggttggtac tggtatggaa cgtgctgttg ccgttgactc cggtgtaact
     6481 gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa
     6541 gttaacgaag acgagatgta tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa
     6601 tacacccgtt ctaaccagaa cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa
     6661 ccggttgaac gtggcgacgt gctggcagac ggtccgtcca ccgacctcgg tgaactggcg
     6721 cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg gttacaactt cgaagactcc
     6781 atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca ccaccatcca cattcaggaa
     6841 ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag agatcaccgc tgacatcccg
     6901 aacgtgggtg aagctgcgct ctccaaactg gatgaatccg gtatcgttta cattggtgcg
     6961 gaagtgaccg gtggcgacat tctggttggt aaggtaacgc cgaaaggtga aactcagctg
     7021 accccagaag aaaaactgct gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac
     7081 tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta tcgacgttca ggtctttact
     7141 cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag
     7201 gcgaagaaag acctgtctga agaactgcag atcctcgaag cgggtctgtt cagccgtatc
     7261 cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat
     7321 cgctggctgg agctgggcct gacagacgaa gagaaacaaa atcagctgga acagctggct
     7381 gagcagtatg acgaactgaa acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa
     7441 atcacccagg gcgacgatct ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg
     7501 gttaaacgcc gtatccagcc tggtgacaag atggcaggtc gtcacggtaa caagggtgta
     7561 atttctaaga tcaacccgat cgaagatatg ccttacgatg aaaacggtac gccggtagac
     7621 atcgtactga acccgctggg cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc
     7681 cacctgggta tggctgcgaa aggtatcggc gacaagatca acgccatgct gaaacagcag
     7741 caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt
     7801 cgtcagaaag ttgacctgag taccttcagc gatgaagaag ttatgcgtct ggctgaaaac
     7861 ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt
     7921 aaagagctgc tgaaacttgg cgacctgccg acttccggtc agatccgcct gtacgatggt
     7981 cgcactggtg aacagttcga gcgtccggta accgttggtt acatgtacat gctgaaactg
     8041 aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg gttcttacag cctggttact
     8101 cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc gtttcgggga gatggaagtg
     8161 tgggcgctgg aagcatacgg cgcagcatac accctgcagg aaatgctcac tgttaagtct
     8221 gatgacgtga acggtcgtac caagatgtat aaaaacattg tggacggcaa ccatcagatg
     8281 gagccgggca tgccagaatc cttcaacgta ttgttgaaag agattcgttc gctgggtatc
     8341 aacatcgaac tggaagacga gtaattctcg ctcaaacagg tcactgctgt cggggtaaaa
     8401 cccggcagcg gattgtgcta actccgacgg gagcaaatcc gtgaaagatt tattaaagtt
     8461 tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc
     8521 agacatgatc cgttcatggt ctttcggtga agttaaaaag ccggaaacca tcaactaccg
     8581 tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt atctttgggc cggtaaaaga
     8641 ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa
     8701 gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag cgtatgggcc acatcgaact
     8761 ggcttccccg actgcgcaca tctggttcct gaaatcgctg ccgtcccgta tcggtctgct
     8821 gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac tttgaatcct atgtggttat
     8881 cgaaggcggt atgaccaacc tggaacgtca gcagatcctg actgaagagc agtatctgga
     8941 cgcgctggaa gagttcggtg acgaattcga cgcgaagatg ggtgcggaag caatccaggc
     9001 tctgctgaag agcatggatc tggagcaaga gtgcgaacag ctgcgtgaag agctgaacga
     9061 aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt
     9121 cgttcagtct ggtaacaaac cagagtggat gatcctgacc gttctgccgg tactgccgcc
     9181 agatctgcgt ccgctggttc cgctggatgg tggtcgtttc gcgacttctg acctgaacga
     9241 tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc
     9301 gccggacatc atcgtacgta acgaaaaacg tatgctgcag gaagcggtag acgccctgct
     9361 ggataacggt cgtcgcggtc gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt
     9421 ggccgacatg atcaaaggta aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt
     9481 tgactactcc ggtcgttctg taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg
     9541 tctgccgaag aaaatggcgc tggagctgtt caaaccgttc atctacggca agctggaact
     9601 gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt
     9661 cgtttgggat atcctggacg aagttatccg cgaacacccg gtactgctga accgtgcacc
     9721 gactctgcac cgtctgggta tccaggcatt tgaaccggta ctgatcgaag gtaaagctat
     9781 ccagctgcac ccgctggttt gtgcggcata taacgccgac ttcgatggtg accagatggc
     9841 tgttcacgta ccgctgacgc tggaagccca gctggaagcg cgtgcgctga tgatgtctac
     9901 caacaacatc ctgtccccgg cgaacggcga accaatcatc gttccgtctc aggacgttgt
     9961 actgggtctg tactacatga cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct
    10021 gactggcccg aaagaagcag aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg
    10081 cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac ggtgaattag tagcgaaaac
    10141 cagcctgaaa gacacgactg ttggccgtgc cattctgtgg atgattgtac cgaaaggtct
    10201 gccttactcc atcgtcaacc aggcgctggg taaaaaagca atctccaaaa tgctgaacac
    10261 ctgctaccgc attctcggtc tgaaaccgac cgttattttt gcggaccaga tcatgtacac
    10321 cggcttcgcc tatgcagcgc gttctggtgc atctgttggc atcgatgaca tggtcatccc
    10381 ggagaagaaa cacgaaatca tctccgaggc agaagcagaa gttgctgaaa ttcaggagca
    10441 gttccagtct ggtctggtaa ctgcgggcga acgctacaac aaagttatcg atatctgggc
    10501 tgcggcgaac gatcgtgtat ccaaagcgat gatggataac ctgcaaactg aaaccgttat
    10561 taaccgtgac ggtcaggaag agaagcaggt ttccttcaac agcatctaca tgatggccga
    10621 ctccggtgcg cgtggttctg cggcacagat tcgtcagctt gctggtatgc gtggtctgat
    10681 ggcaaagccg gatggctcca tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct
    10741 gaacgtactc cagtacttca tctccaccca cggtgctcgt aaaggtctgg cggataccgc
    10801 actgaaaact gcgaactccg gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct
    10861 ggtggttacc gaagacgatt gtggtaccca tgaaggtatc atgatgactc cggttatcga
    10921 ggggggtgac gttaaagagc cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga
    10981 cgttctgaag ccgggtactg ctgatattct cgttccgcgc aacacgctgc tgcacgaaca
    11041 gtggtgtgac ctgctggaag agaactctgt cgacgcggta aaagtacgtt ctgttgtatc
    11101 ttgtgacacc gactttggtg tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca
    11161 catcatcaac aaaggtgaag caatcggtgt tatcgcggca cagtccatcg gtgaaccggg
    11221 tacacagctg accatgcgta cgttccacat cggtggtgcg gcatctcgtg cggctgctga
    11281 atccagcatc caggtgaaaa acaaaggtag catcaagctc agcaacgtga agtcggttgt
    11341 gaactccagc ggtaaactgg ttatcacttc ccgtaacacc gaactgaaac tgatcgacga
    11401 attcggtcgt accaaagaaa gctacaaagt gccttacggt gcggtactgg cgaaaggcga
    11461 tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt
    11521 tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg atcgacggcc agaccattac
    11581 tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg
    11641 taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc gttgatgctc agggtaacga
    11701 cgttctgatc ccaggtaccg atatgcctgc gcagtacttc ctgccgggta aagcgattgt
    11761 tcagctggaa gatggcgtac agatcagctc tggtgacacc ttggcgcgta ttccgcagga
    11821 atccggcggt accaaggaca tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc
    11881 tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa
    11941 agaaaccaaa ggtaaacgtc gtctggttat caccccggta gacggtagcg atccgtacga
    12001 agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg
    12061 tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca
    12121 tgctgtgact cgttacatcg ttaacgaagt acaggacgta taccgtctgc agggcgtaaa
    12181 gattaacgat aaacacatcg aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt
    12241 taacgcgggc agctccgact tcctggaagg cgaacaggtt gaatactctc gcgtcaagat
    12301 cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca acttactccc gcgatctgct
    12361 gggtatcacc aaagcgtctc tggcaaccga gtccttcatc tccgcggcat cgttccagga
    12421 gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct
    12481 gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt accggttacg cgtaccacca
    12541 ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct gcaccgcagg tgactgcaga
    12601 agacgcatct gccagcctgg cagaactgct gaacgcaggt ctgggcggtt ctgataacga
    12661 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggtgtt
    12721 attcagaggg caaattcttc acggaaaata ttaagaaagg cagggcgaaa cgaggaagaa
    12781 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    12841 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    12901 ccgtctcata gtctttgcga ggcgcgtccc agatagctgt cccagtcttt ccataccggc
    12961 tgcaaaccct gagcggttaa cacggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13021 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13081 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13141 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13201 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13261 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    13321 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    13381 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    13441 tgttttttgc ctttcaaatg atggcgggta taagtcgcct cgtgatatgt ctcctgataa
    13501 accatcaccc catccagacc aagttgcttt aactcggcgt attccgtctc cgccagcggt
    13561 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    13621 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    13681 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    13741 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    13801 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    13861 tgttgggcca gttgttccag atagccactg gcggcaggcg ataacagcgc catcatgtcg
    13921 tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt tttgccgttg
    13981 atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gattgctgaa ggttttcatg
    14041 ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaataa ctgcggctgc
    14101 ccggtccgga ctgacgtgcc agcaggccta cttctaccgc cagacgaaat gccttcgcca
    14161 tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14221 tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    14281 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    14341 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    14401 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg
    14461 cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcca
    14521 attttagcca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    14581 ccgctgtttt cgccccggaa gtattcggca gcagggccac acccgccgcg ataagcggtt
    14641 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    14701 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    14761 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    14821 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    14881 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    14941 tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca tcggttgatc gttaaacagg
    15001 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15061 ccactggctc gacttaccgt cgaacagtcg gagttctccc gcaggcgtct ctataccgct
    15121 taataactta atggcttcca gtgcctgcaa agtgcccatc accccgacca ccgggccaac
    15181 cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta tctggccaca ggcagcggta
    15241 acacccctgc tcccagggcg gtgtcagtac catcaactga ccgccaaatc cgaccgcgct
    15301 ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct ggcgagtcgc
    15361 catattgtcg gtacagtcga gcaccacatc ggcttgtgca accgcatctt tcaacgcctc
    15421 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    15481 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga ggatttgtcg
    15541 ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    15601 ccctgccagg tatagcgcag caggtgtacc cagcccgcca agaccaataa tcagcacctg
    15661 gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagaa tttggcggct
    15721 ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    15781 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac gacggcgata ctgccgacac
    15841 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    15901 aatccgccag gcgctcaaca tgccgtgcca actgttccag cccctgcggt gcagaaggca
    15961 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16021 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc cagccgcagg cctgccgcgc
    16081 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16141 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16201 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16261 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    16321 ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    16381 ttcctcctta tgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    16441 cattcccact tcaatggttt gcgcggcggc gtaatcgcgt acttcctggc tgattttcat
    16501 cgagcagaat tttggcccac acatggagca aaaatgggcg actttgccgg actcttgggg
    16561 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    16621 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    16681 atgccctttt gccaggtcag cagcgtgggc ggcaatctta taagtgataa gcccctgctt
    16741 aacatcttct ttattgggca gacccagatg ctcttttggc gttacgtaac agagcatcgc
    16801 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    16861 tgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    16921 ctcctcggtc atattgcggc ggatcatctg catcggcaca tggcctgggc cttcaatcat
    16981 cacctgtacg tcatattccc aggcgatttt ggtcagttcg cccagcgtat gcaattcggc
    17041 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17101 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17161 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17221 accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg catggatagt
    17281 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    17341 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    17401 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    17461 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    17521 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    17581 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    17641 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gatgtgcgcc
    17701 aaagctcatt cccggatgct ggtggcgtaa aacctcgcta cggatgcgct cgcggcccat
    17761 attctcgcgg atggcgatga attccatttc cggcgtgatg atgccctggc gggcgtagtg
    17821 cagttgggtg acacggcgtc ctgttttggc gcgttttggc gttaataagc cgctaaaacg
    17881 tagctcgtcg agaccatcat ctgccagccg cgttctggtg taatcggaac tgcgcacggt
    17941 aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18001 ctgcacgtta atggcgatct gcggatcacc atacgggccg gaggtgtcgt agaccggaat
    18061 cgcttcgttt tcttcgtact gcggctgttc tttgctaccg ccaattagcg tcgggctaag
    18121 ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatat aaatgcgttt
    18181 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18241 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    18301 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    18361 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    18421 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    18481 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    18541 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    18601 aaaacctgtt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    18661 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    18721 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    18781 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    18841 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    18901 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    18961 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19021 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19081 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19141 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19201 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt taatacaaca gcagcggcgt
    19261 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgggctgtt tcaactggcc
    19321 ggacgtggcg tgcaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    19381 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gtagccattg ccgtgagcgt
    19441 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgctcctc
    19501 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    19561 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    19621 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    19681 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    19741 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    19801 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    19861 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    19921 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    19981 tatctaccgg aatataaagc cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20041 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20101 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20161 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataaa
    20221 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    20281 cgtcacgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    20341 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    20401 tatgccgatc cgcaggcgct gcacgctctg ctcgataaac tggcgaaaag cgtcactttg
    20461 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    20521 ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    20581 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag tcacgctgtt taccaaaggc
    20641 ggcggacagt ggctggaagc catggctgaa accggttgcg atgcgctggg cctcgactgg
    20701 acaacggaca tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    20761 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    20821 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    20881 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacag
    20941 tatcaccgct aagagcctga tccatcaggc tattttattt gccattttga ggccgggcag
    21001 tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt tccgcgctgt ccggcctcaa
    21061 actgccttca acaattacgc ctgttgaacc aggctctaag gagcgattat ggatctcgcg
    21121 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tctgcgagga tcgactcgat
    21181 aaagatccac cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    21241 acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg agctggtgga gtataaagtt
    21301 gcccgcatcg ccaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    21361 gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt
    21421 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggctt attggtggat
    21481 gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta aattcgaacc gctctccagc
    21541 aaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    21601 agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc atcgggtcag cgtggacagc
    21661 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    21721 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    21781 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    21841 catctgcgtc tggagcgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    21901 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    21961 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta
    22021 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    22081 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    22141 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    22201 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatgg gcgatgaaga gcttaaagaa
    22261 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    22321 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgagtcgggt
    22381 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    22441 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    22501 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    22561 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    22621 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    22681 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    22741 ggtaccttca aagtgaacca tcgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    22801 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    22861 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    22921 ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc
    22981 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    23041 cgcatctggc gaaaagacag cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg
    23101 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    23161 aatatcaatg tttacagcaa accgccggtg aacattcgcg cgcgttttga cgatcgcggc
    23221 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    23281 atcgatttat accgttatcg tgctgatcag atccgccaga ttagcgatgc cttacgtcag
    23341 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    23401 gaagggcaaa ccattaaacc tgatttagat tcgcaggcga tagcgcatat cgagcgccgc
    23461 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    23521 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    23581 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    23641 ttccggctcc gcgcggaata cccgcttcag ccatcgcgat atctcgtttc acccgtaact
    23701 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    23761 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    23821 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    23881 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    23941 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    24001 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    24061 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    24121 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    24181 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    24241 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    24301 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    24361 gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc
    24421 tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    24481 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    24541 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attcccctca
    24601 ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg atagcgcaga
    24661 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgta
    24721 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    24781 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttattctctt
    24841 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    24901 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    24961 gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga ttaaaggact
    25021 ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt
    25081 gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc gagttgctgg aactggttaa
    25141 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    25201 gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg ccaacgacac
    25261 attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctctatct
    25321 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    25381 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    25441 agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg ggctggcggt
    25501 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc aggagggaaa
    25561 aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    25621 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    25681 caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    25741 ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    25801 atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat tccggtgctg
    25861 attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac tggggcgcag
    25921 gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga aaaggcgctc
    25981 gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca gttcggtatg
    26041 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    26101 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    26161 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    26221 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    26281 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa
    26341 attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa
    26401 gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat tgcggcgacc
    26461 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    26521 ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tatccctctg
    26581 ctggctggcc attttcttca gcgctttgcc gagcgtaatc gcaaggcggt aaaaggcttt
    26641 acgccacagg cgatggattt gctgatccac tacgactggc cgggaaatat tcgtgaactg
    26701 gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag
    26761 ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga tattcagccg
    26821 ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg agaaaacggg cggcaacaaa
    26881 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt
    26941 tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt tccggcagaa gcaatcatcc
    27001 cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg cttctgccac ggtatgaccc
    27061 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    27121 tttgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag gccgtggatc
    27181 acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gctgagagaa
    27241 gcacgttcat cccactctga cgttttctcg tccagtttgc cttcacaggc cgccaggcag
    27301 agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg atcgccaaag
    27361 cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag gcccgcatag
    27421 agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg ccagatgata
    27481 cgttccatgg tgcgctgatg aacttcatcg gttactaccg gcgcggggga gtaagcgccc
    27541 atcccgccgg tgtttggtcc gttatcttta tcgcctacgc gtttgtgatc ctggctggta
    27601 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccatcg
    27661 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata
    27721 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    27781 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata caccagcgca
    27841 ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg ggccaggaaa
    27901 tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg gccgaagatt
    27961 ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    28021 acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc
    28081 acgccgatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt acccggagca
    28141 gcaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    28201 ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg aagtggcgca
    28261 tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    28321 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    28381 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    28441 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    28501 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaatttcg
    28561 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgttgggtga
    28621 cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc
    28681 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    28741 cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa
    28801 taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    28861 ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt
    28921 aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc ttcacaatca
    28981 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    29041 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    29101 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    29161 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    29221 caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    29281 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    29341 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    29401 catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata tcgatgttct
    29461 caaccgcatc ttccagcgag cagccttcac gagcaacagt ctgggcgaac ggatacaggt
    29521 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    29581 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    29641 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    29701 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    29761 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    29821 gttgttgcat ggtaaatccc ctgggtttga ctattacaga gagcgttagc tgaatttttc
    29881 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    29941 aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca attttgacag
    30001 tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact ctgtgcgtaa
    30061 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    30121 tctattgcgg cctgcggaga actccctata atgcgccttc atcgacacgg cggatgtgaa
    30181 tcacttcaca caaacagccg gttcggttga aga
//
LOCUS       DAJTNP010000042        29645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTNP010000042 DAJTNP010000000
VERSION     DAJTNP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1..443)
                     /locus_tag="OO160_002060"
                     /pseudo
     CDS             complement(1..443)
                     /locus_tag="OO160_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(418..1218)
                     /gene="otsB"
                     /locus_tag="OO160_002061"
     CDS             complement(418..1218)
                     /gene="otsB"
                     /locus_tag="OO160_002061"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5524188.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1385..2371)
                     /gene="araH"
                     /locus_tag="OO160_002062"
     CDS             complement(1385..2371)
                     /gene="araH"
                     /locus_tag="OO160_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5524189.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2386..3900)
                     /gene="araG"
                     /locus_tag="OO160_002063"
     CDS             complement(2386..3900)
                     /gene="araG"
                     /locus_tag="OO160_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5524190.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3970..4959)
                     /gene="araF"
                     /locus_tag="OO160_002064"
                     /pseudo
     CDS             complement(3970..4959)
                     /gene="araF"
                     /locus_tag="OO160_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5756..6259
                     /locus_tag="OO160_002065"
     CDS             5756..6259
                     /locus_tag="OO160_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5524191.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6339..6590)
                     /locus_tag="OO160_002066"
     CDS             complement(6339..6590)
                     /locus_tag="OO160_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5524192.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6705..6791)
                     /gene="azuC"
                     /locus_tag="OO160_002067"
     CDS             complement(6705..6791)
                     /gene="azuC"
                     /locus_tag="OO160_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5524193.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7054..7377
                     /locus_tag="OO160_002068"
     CDS             7054..7377
                     /locus_tag="OO160_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5524194.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7548..8045
                     /gene="ftnA"
                     /locus_tag="OO160_002069"
     CDS             7548..8045
                     /gene="ftnA"
                     /locus_tag="OO160_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5524195.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8082..8321)
                     /locus_tag="OO160_002070"
     CDS             complement(8082..8321)
                     /locus_tag="OO160_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5524196.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8511..9722
                     /gene="tyrP"
                     /locus_tag="OO160_002071"
     CDS             8511..9722
                     /gene="tyrP"
                     /locus_tag="OO160_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5524197.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9784..10449)
                     /gene="yecA"
                     /locus_tag="OO160_002072"
     CDS             complement(9784..10449)
                     /gene="yecA"
                     /locus_tag="OO160_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5524198.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10645..10731)
                     /locus_tag="OO160_002073"
     tRNA            complement(10645..10731)
                     /locus_tag="OO160_002073"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10695..10697),aa:Leu,seq:taa)
     gene            complement(10744..10817)
                     /locus_tag="OO160_002074"
     tRNA            complement(10744..10817)
                     /locus_tag="OO160_002074"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10783..10785),aa:Cys,seq:gca)
     gene            complement(10872..10947)
                     /locus_tag="OO160_002075"
     tRNA            complement(10872..10947)
                     /locus_tag="OO160_002075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10912..10914),aa:Gly,seq:gcc)
     gene            complement(11099..11647)
                     /gene="pgsA"
                     /locus_tag="OO160_002076"
     CDS             complement(11099..11647)
                     /gene="pgsA"
                     /locus_tag="OO160_002076"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5524199.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11704..13536)
                     /gene="uvrC"
                     /locus_tag="OO160_002077"
     CDS             complement(11704..13536)
                     /gene="uvrC"
                     /locus_tag="OO160_002077"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5524200.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13533..14189)
                     /gene="uvrY"
                     /locus_tag="OO160_002078"
     CDS             complement(13533..14189)
                     /gene="uvrY"
                     /locus_tag="OO160_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5524201.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14485..14661
                     /locus_tag="OO160_002079"
     CDS             14485..14661
                     /locus_tag="OO160_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524202.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14648..14872
                     /locus_tag="OO160_002080"
     CDS             14648..14872
                     /locus_tag="OO160_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5524203.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14905..15525)
                     /gene="sdiA"
                     /locus_tag="OO160_002081"
                     /pseudo
     CDS             complement(14905..15525)
                     /gene="sdiA"
                     /locus_tag="OO160_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15755..16507)
                     /gene="yecC"
                     /locus_tag="OO160_002082"
     CDS             complement(15755..16507)
                     /gene="yecC"
                     /locus_tag="OO160_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5524204.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16504..17172)
                     /gene="tcyL"
                     /locus_tag="OO160_002083"
     CDS             complement(16504..17172)
                     /gene="tcyL"
                     /locus_tag="OO160_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5524205.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17187..18173)
                     /gene="dcyD"
                     /locus_tag="OO160_002084"
     CDS             complement(17187..18173)
                     /gene="dcyD"
                     /locus_tag="OO160_002084"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5524206.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18278..19078)
                     /gene="tcyJ"
                     /locus_tag="OO160_002085"
     CDS             complement(18278..19078)
                     /gene="tcyJ"
                     /locus_tag="OO160_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5524207.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19166..19717)
                     /gene="fliZ"
                     /locus_tag="OO160_002086"
     CDS             complement(19166..19717)
                     /gene="fliZ"
                     /locus_tag="OO160_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5524208.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19763..20482)
                     /gene="fliA"
                     /locus_tag="OO160_002087"
                     /pseudo
     CDS             complement(19763..20482)
                     /gene="fliA"
                     /locus_tag="OO160_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20647..22299)
                     /gene="fliC"
                     /locus_tag="OO160_002088"
     CDS             complement(20647..22299)
                     /gene="fliC"
                     /locus_tag="OO160_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5524209.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22565..23971
                     /gene="fliD"
                     /locus_tag="OO160_002089"
     CDS             22565..23971
                     /gene="fliD"
                     /locus_tag="OO160_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5524210.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23996..24406
                     /gene="fliS"
                     /locus_tag="OO160_002090"
     CDS             23996..24406
                     /gene="fliS"
                     /locus_tag="OO160_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5524211.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24406..24771
                     /gene="fliT"
                     /locus_tag="OO160_002091"
     CDS             24406..24771
                     /gene="fliT"
                     /locus_tag="OO160_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5524212.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24849..26336
                     /gene="amyA"
                     /locus_tag="OO160_002092"
     CDS             24849..26336
                     /gene="amyA"
                     /locus_tag="OO160_002092"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5524213.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26370..26783)
                     /locus_tag="OO160_002093"
     CDS             complement(26370..26783)
                     /locus_tag="OO160_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5524214.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26970..28175
                     /gene="yedE"
                     /locus_tag="OO160_002094"
     CDS             26970..28175
                     /gene="yedE"
                     /locus_tag="OO160_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5524215.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28172..28405
                     /gene="yedF"
                     /locus_tag="OO160_002095"
     CDS             28172..28405
                     /gene="yedF"
                     /locus_tag="OO160_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5524216.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28512..29182
                     /locus_tag="OO160_002096"
                     /pseudo
     CDS             28512..29182
                     /locus_tag="OO160_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29222..>29645)
                     /locus_tag="OO160_002097"
     CDS             complement(29222..>29645)
                     /locus_tag="OO160_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5524217.1"
                     /translation="MIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYE
                     MRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADV
                     NGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7373 a         7665 c         7054 g         7553 t
ORIGIN      
        1 accaatgcga ttattcaccc cacgtttgcg taattcatgc gcaaagggca acaggtgata
       61 atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat ctgccagcaa
      121 cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgagccg
      181 ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc
      241 ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttagcgg ctgatcctca
      301 ttccctgttt caccactcca gccaaaccac agtccgcctg cggctttcag tgcccccagt
      361 atgccaacgg caaggccacc ggcactggcg gcgtgctcgt ctggtggtgc aatccggtta
      421 gatactacga ctaaacgact catagtcatc actcctgtta ttttctcttt tttgttgtaa
      481 tgcggcggtt atcatttcaa gccagctcca gacatccggc acacccgcca gtcgccatga
      541 tgcctgagtt gcacctgtgc caatttttac tgacattccg cccagtcggt taacgactgc
      601 gaagccagat tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc cgataaaagg
      661 agcttcctgc ataaaggctg caatcgcttc acctttactg gtacctctcg gtttgatctc
      721 gacaacacac tttccctgct gtaacgccat ttgtggccag atctgagtaa tacgttgcgc
      781 taatgtcatt aatgcgtctt catgctgcgg agcctgacga taatgcagcg caaaagccat
      841 ccctttcgcc tccagctccg cgccgggata ctgagcgatg accgtatgca gttgcacgct
      901 aatatcacgc gcaatcgcat ccggcagatg aacgatatgt gttttaccat tgatgtcacg
      961 gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca gtgcgtcaag
     1021 ctccaccatt gagcgccctg atatcaatgc caatgcacca tcacttgcgg ttgccagtag
     1081 ctgtagtcct tgcagaatat tgtcaggcac gacgacctga tcgggatgcg gtttgatttc
     1141 cgccagcgtt ccatcaagat caaaaaacca ggcatatttc gcggatagtt caggggtttc
     1201 ggttaacggt tctgtcaccc ggctctcctc cttctctttt cttattgtgt tgcgcagacc
     1261 gcgctttaca gacatgtaag tatagacagt gtgacggagg tcgccatttg caacgaagaa
     1321 cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac gttaagttgt tgtggaaaaa
     1381 agcatcagac agtgcgcttc gctttttgct tgtaacggtc gaagatcacc gctgccagca
     1441 ggattaagcc gcgaaccacg tactgcgcga aaggagaaat attaagcagg ttcatggcgt
     1501 tttccacggt gcctaaaatt aagatacccg ccaccacata tgagattttt ccgatgccac
     1561 ctttcagaga aacgccacct aaaacgcagg cggagataac aataagctca taaccaatcg
     1621 acgtcattgg ctgcccacta gtcatacgtg aagccagaat aattccggct atcgcagata
     1681 ccaggccaga gagaacaaag ataataattt tggtgcgaac aaccggtaca cccgccagac
     1741 gcgcagcctc ttcgttcccg ccaatcgcca gggtattacg accaaaggtg gttttattca
     1801 gcagcaaacc aaagataatc agacacgcga cggtgagcca gattggcgca ggcagaccga
     1861 accagttagc gtaaccaagg gcaaagaagc tttcatcttc gataccgacc gctttaccgt
     1921 ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtc gtgatcagag
     1981 catttatttt cagtttggcg ataacaaagc cattgaccag gccacagaga acgcccagca
     2041 acaaacccgc tgccacgcca atccacaggc tttcagtcag gttgataacc accgccgtgg
     2101 tgacacccgc acaggcaatt acggaggcga cagaaaggtc aaagtcaccg gaagcaaggc
     2161 agaacaacat gccacacgcc accatccccg acatggaaat tgccaggccc aaccctttca
     2221 tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca ggcgataaag agcaccgcaa
     2281 acaccaccag catgccgtac tgatcccaga tacgcccgaa gctgaatgac gacttaggtg
     2341 cgccagaccc cgatgtagaa acagaagaca tcatactctc cttactcagg caactgcctg
     2401 gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct cgtgtaacaa
     2461 ttcaccggcg atttcgcctt cccgcatcac cacaatccgg tcggcaacgc cgaggacttc
     2521 aggtaagtcg ctggaggcaa acagcaccgc cacgccctgc gccgccagcg cataaattac
     2581 gttatatatt tcgtgcttag cgccaacatc aatgccgcgc gtaggttcat ccagcaaaat
     2641 gaccttcatc tcttccgata accagcggcc cagaatggct ttttgctgat ttccgcctga
     2701 gagattcatg atcagttgct ccgcgcccgg cgttttgatg ttgagcgaac gaatgtgctg
     2761 atcggcattg ttttcttccc aactgttgtt gattacacaa ccgccgagca catgtttgcg
     2821 tctggcactg atgttgatat tgtcgcgaac ggagtgcacg ggaataatgc cttccgcttt
     2881 gcgagcttcc gggcagagca tcatgcctgc ggcaatggcg tggctcggtt tacgaatatc
     2941 gatcggctgt tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc caaacaagcc
     3001 tttcattaat tcgctacgcc ccgcccctac cagaccaaac agcccaacaa tttcaccact
     3061 gcgaaccgcc agacttattg gcgtacgcac gcctagtgct ttcacagcat caagacgcag
     3121 gcgctcctcg ccataactac gaggttgcca gccgtagata tcgccaatgt cgcgcccgac
     3181 catcgcctgc accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg ttttgacata
     3241 acgtccatct ttaaagacag tgatggcatc gctgagggca aatatttctt ccatacggtg
     3301 agaaacgtat aagatcaccc gcccctcttt tcgcagttca cgaataacgc ggaaaagatt
     3361 gccgatttcg cgggcagaaa gggagctggt tggctcatca aaggcgataa ttttagcgtt
     3421 acgcgccagc gctttggcga tttcaaccat ctgccactga ccaatggcga gatatttcag
     3481 cggcgtgtcc gggtcaatat ccataccaag atgtttaagt tgtaaacccg cctcataatt
     3541 cagcaatgag cgattcacaa tgccgccttt atgcggcagc tggccgagat agatgttttc
     3601 cgcgacggtc atttccggca cgagatgcag ttcctggtaa ataatcgcca cacccgcgtt
     3661 aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta cagaacccgt
     3721 ggttggcgca tagttgccgc tgaggatttt taagagagtt gattttcctg cgccattttc
     3781 acccatcaac gcatgaacct gaccggcata gcagtcaaaa ctaatatccg tcagcgcctt
     3841 aacgccggga aacgttttac cgatgccgcg aaatgagaga tacggggtag actgttgcat
     3901 aacgtctccg tgaatcactg gtcgttcaca ccccctctgc atcatgcaga ggggaatttt
     3961 tccggcaaat tacttaccgc ctaaaccttt tttctccagt tcttctttaa agttgtcacg
     4021 cgtgatcagt accacgtctg taacttcggt aaattttggc gtttcaacgt cttttgctac
     4081 ccagttgtaa agcatttcgc tggatttata gccatgtacg tccgggcttg gcagcaggga
     4141 accgtagaag ccggttgcct gtgctttaga cagttcgctt actgcatcca caccgttaat
     4201 accaataccg atgatatcgg ctgctttaaa gccctgacct tcggttgcac gtacgccgcc
     4261 aagtacggtg ctgtcgttca taccgacgat cagccaatgt ttaacttccg gatgttgaac
     4321 cagcattgag ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg taggtacctg
     4381 ataaatttgt ttttccggga atcctgcagc tttcagtgcg tccatagaac ccgttgtacg
     4441 gcggcgggcg gtatccagtt cgttggcggt aatcgccatc actgcgcttt ctttgacatc
     4501 ccagccacgt ttctgcatct ctttatacag ttcctagccc tgacgttcgc caattttagt
     4561 cgccgccatc atcaccagag gaacggtatc cattggctta cctttggcgt taacaaactg
     4621 gtcatccact gcaatgactt tcatatcgta gccacgcgct ttcgcgacga tggcagagcc
     4681 gagtttcggg tccggagtac aaataacgaa accttttgcg ccactggctg ccaggctgtc
     4741 gatcgcgttc aatgtttttt cgccatccgg cacggcaatc ttaataacct caaaccctaa
     4801 atccttcccg gctttatcgg caaacttcca ttcggtctgg aaccacggct cttccggttg
     4861 cttcaccaga aaaccgagct tcaggttctc cgccatagcg gattgtgaca taacggctgc
     4921 cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg gttctctcca gctttagtgt
     4981 cgttttgtgt agggcaaaaa cgaatgacat tcgttaaatt aatcggaaaa caaagcatta
     5041 ccttttatct aaaagataag tgaccgtgtt gacatagttt tagcgagaaa ttaattctcc
     5101 ataggagagc aatatcacat cgcagaatta cagtgagaac gtgcataaat ttagcgggaa
     5161 aagacataag gaaaagccaa tttgtcagac aaattgttga atgcacagca gattaatcca
     5221 taagattagc ctggaaatcc ttgttgtctt cggtacccat gcgggatgtc ttctttttaa
     5281 ccagtcaata ggccgcatta cctggcgttg agtttttgaa atggtgtaat aaccacaact
     5341 caaagatgtg gaaaatgcgc gtcattcatt tcgccattaa ttatcactgt gctcattaat
     5401 taacagaaca cgtataatga gagccatctc gcaaaaatga aaaaacgttt tataaaatca
     5461 tcacttcatc atgaattcaa attcatcgat taatatcaac aagatacaaa aagcactatc
     5521 attaaaattc attgcattta cattgatttc accaatgaaa tgtaaaaata tataaacttg
     5581 atgatttaag cattttctta taaccgttca gacgttattc ttatttcaga tcatcgtcag
     5641 aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt
     5701 atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc
     5761 aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa
     5821 tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt
     5881 ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa
     5941 gagtgtcggc gctaccccca tcgttaaagc cattgatgtt ccaggtgaaa aactgaactc
     6001 tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcaa gcactctggc
     6061 gcagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga
     6121 tctggaaaaa gaacagcagc atgatggcct gttgctgcaa accattcttg atgaagtgcg
     6181 cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt
     6241 gtcacaccag ctgcattgat catacatcgg cgctaataca ttgcgccgat gaagtttttg
     6301 agaaaccgct gcctcatctg tttgaagcag cggttttttt aatgggattc accctgtggg
     6361 gtaaacttga gttcaatcag cgcgatggct ttttggattg cccgcatggt gaccgggtct
     6421 gcggcagcgg gatgatgagt aaagtcgata ttcttcagct gactggacat tttttcacga
     6481 acttcaacgg gcgcgattac atcgagaaca tccagaattt gtttgataac caactggcaa
     6541 gctaccacat cagaaaccag ttcctgatcg gcattcagcg gctgggacat cgtaaactcc
     6601 tgatagcatt ttgaaagccg ttatagtagc ggcttcacat cttcagcgat agtcacatcc
     6661 accgtcatca ggacacaaaa aaacctgccg gagcaggttt tttgttatcg gaacatattg
     6721 cctggcggta cgtctttgaa cgtcttgcaa tagttattga acatactttt caggattttg
     6781 cgcagtttca tcgcggcact ccgaccattt gttatacagg tgttattgtc tttgcgctta
     6841 taatatgaca accatcacaa aaatcaatct ttatgtgata caaatcacat aaatacccct
     6901 ttaatgttat aaaaatgata atcaaaaaac agccccccta tttctgacac ctacagatgg
     6961 caagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg taacgcgtaa
     7021 aatagtgctt tctcttactc ttctggctgg accatgagac ttctgattct gactctttca
     7081 ttaataacgc tggcggggtg tacggttact cgccaggccc atgtgagcga agttgacgcg
     7141 gcaacaggta ttgtacggtt ggtttatgac caggcttttt tgcagcatgc ccataccgat
     7201 cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta tacccacgcg
     7261 atcccctttg gtcagccagt aggcaactgt agcctttttg cgggttcctt atgtttgaat
     7321 acggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt tctctaaata
     7381 ttttaatcag cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg ttttattccc
     7441 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
     7501 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
     7561 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
     7621 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
     7681 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
     7741 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
     7801 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
     7861 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
     7921 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
     7981 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
     8041 actaatgctc tccggcggca ggtttctgcc gccgtttcaa atcagtgacg gcaaatctta
     8101 ctctgatcgg tcgaaaaacc ttcttctgaa ggtataaatt tgccttttgc tgccagaaac
     8161 gctaccagct ctcccgctgt caccccttct gccgagcacg tgtgaaaacg tgcctgttca
     8221 ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg tatattgctc gcctgattca
     8281 atcatcatat ttaacacttc atgaccgtga atagagtcca tcgttcctcc tcaaaaaaag
     8341 cctagcgtag cgattaccgc ttatgaagac tttgcgccag cgcaggactg aatgctttta
     8401 ttgtacattt atatttacac aatatgtaac gtcggtttga cgaagcagcc gttatgcctt
     8461 aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca
     8521 gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc atgctggcaa
     8581 tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg attgggcttt
     8641 gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat gttccggcag
     8701 ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa tggctgacgg
     8761 gcttcagtat gatgttctta atgtatgctc tgactgcagc atacatcagc ggtgccggtg
     8821 aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg
     8881 tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg ctggtcgatt
     8941 tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg ctggtattgc
     9001 tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag gggctggcac
     9061 tgtctgcaat cccggtgatt tttacgtcgt ttggttttca cggtagcgtg ccgagtattg
     9121 tcagctatat ggatggcaac attcgtaagc tacgctgggt gtttataatc ggtagtgcca
     9181 tccccctggt ggcatatatt ttctggcagg tggcgacgct tggcagcatt gattcaacaa
     9241 cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag gcgttacgcg
     9301 aaatggtggc ctctccgcat gttgagctgg cggtgcattt atttgctgat ttagccctcg
     9361 ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat ttgtttcagc
     9421 gttcaaatac cgttggtgga cgattgcaaa ctggtgcaat tacctttctg ccgccgttgg
     9481 cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcgc
     9541 tggcggtact ggcattgatt atcccttcac tattgacctg gcaaagcaga aagcacaatc
     9601 ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg
     9661 gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca gaagtggggt
     9721 gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac ccaatgtgac
     9781 ctcttaatgc aggcagcact gcttaaattt cttaccactg ccgcacggac aaggatcgtt
     9841 acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc
     9901 catccagtat gcatgtaaat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa
     9961 cgcttctggc gacattttct ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat
    10021 cgcctccagc gctggtttta aagaatcggg caacgttgac cagtcagaaa gtgccacgcc
    10081 ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat caacttctcg
    10141 caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg cggtatcggc
    10201 catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg cccaacgcgg
    10261 cacatagtca gccccacccc acacggcaac cagccactgt tctggttcaa tctcttgcgg
    10321 agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga tggcgtggtc
    10381 agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc
    10441 cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt
    10501 ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag gagggtgtaa
    10561 cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact gcttattact
    10621 ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc gcacagcgcg
    10681 aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg ctcgggaaga
    10741 aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc gctacataac
    10801 cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct tgacgccgat
    10861 gtcttttaaa ctggagcggg aaacgagact cgaactcgag acctcgacct tggcaaggtc
    10921 gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc acttgatttt
    10981 attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta cctggcgcga
    11041 tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattacgc cgaaacgatc
    11101 actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac cacagagtca
    11161 gtaccgcagc cacaaagaaa agtgcaatac cgacgtactc aacccaaatg ttcggacgcc
    11221 acagcagcca ggccaacgcc accatctggg cagtggtttt cactttccca atccaggaga
    11281 cggccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca gaaataataa
    11341 tttcacgggc gatcatcgtt gccgccggta atgtcaccca ccagctgtga taatgctcgg
    11401 ttaccagcac catggcgata gccacgagaa ctttatctgc cacagggtca aggaaagcac
    11461 caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg
    11521 ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc agataaaaga
    11581 ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta gggatattaa
    11641 attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt tgctacagag
    11701 acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa tacccggcac
    11761 ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca tatatttcaa
    11821 caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac tggtattttt
    11881 gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt catcgcgaat
    11941 atgctggata acatgcagcg cgggtgaatc tggcggcaaa ctaaatccct caccttccgg
    12001 ctcaaagaac agcgtttcca gtccagcctt acgatctgct cctttggcaa cgccaagtag
    12061 cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt tcgcctgcgc
    12121 aagctggcct ttgccgccgt cgataaggat cacatccggg atcttactgt cgtcaatggc
    12181 tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc ccggcgtgat
    12241 gcctgtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat caaacaccac
    12301 acaggaagcg acggtttgtt cgcccatggt atggctgatg tcaaagcact ccatccgctt
    12361 cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa cggtagattg
    12421 ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt tcagataacg
    12481 cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca gttctgaaag
    12541 ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct cacccggtaa
    12601 ggtgcgcatc tggctgcctt gtaaatagaa ctgacctacg aaggtttcta ccacctcgct
    12661 cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca ctttgccctg
    12721 acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac caataacgtc
    12781 aaggtcgtcg ccggtattgg aaacaaactg tttttcggtg acgcgtcgca ccgcctgaat
    12841 ttggtcgcga atacatgccg cttcttcaaa ctccagattc tggctggcag tttccatacg
    12901 gctaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc gcacatactc
    12961 gacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc ccagacagcg
    13021 ccctatctgg tattgcagac acggacgcga gcgattgcga taaacactat tttcgcactg
    13081 gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca cgtacggcat agccattcgg
    13141 gaacgggccg aaatattcac ctttggcatg ctttgcacca cgatgcatcg ccagacgcgg
    13201 gtgggtatcg ccactcagga agataaaagg ataagattta tcatcgcgta gcaaaacgtt
    13261 gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt cggtttctgt
    13321 atgcgtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg ttttgcgcga
    13381 agcgaggttg ctgcggaaat agctggaaag ccgttttttc aggtctttcg ctttgccgac
    13441 atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc tggttacggt
    13501 ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt ctccgcatta
    13561 cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat gtttagttta
    13621 ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag ctgttctgag
    13681 atctcattga ccttctggcc cttagtgatc atcagcataa tctgcaattc acgttcagac
    13741 aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc catttgttga
    13801 gcgatatcag aagcaatgta acgctgccct gaatagacag aacgaatcgc actcacgact
    13861 tcctgcggag ccgcgccttt gctgaggtag ccagcagcac ctgcctgcat gactttcgct
    13921 ggtaaagggt tttctgtatg gaccgtaagc atgatgattt taacatcagc tgtggaacgc
    13981 gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc cattagcacc
    14041 acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga tgcctctccg
    14101 acgactttta tacccttaat atcttccaga atgcgtcgta tccctgcgcg caccagttcg
    14161 tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg gataacgcta
    14221 ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac gttaaaaatg
    14281 gtgggtattc gatttttctc tcgtttttgt caattcgatt gtccacagtt atggtcacgc
    14341 ccgtcaccaa cggcttaccg gatcgtttca cagtaagccc tacgatgaaa tgtgacaaaa
    14401 attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat taattaaccc
    14461 gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta tattaacgta
    14521 ttttatagca ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt
    14581 atggtatact ccgccgcctt aacatttttc accacaaatt ttcattgcaa catgacgagg
    14641 aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga
    14701 agtctcctgc ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc
    14761 gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc
    14821 tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac
    14881 cggctgagag cgtgcctttc agccggttat tgacaaaatc atcgctatct ctgctgatgt
    14941 cttcccttcc gccgtccatt tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg
    15001 cgtcatcact atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa
    15061 ctgcattttt aattgcagtt catcactaag aatgggtatt tcgcgcgtgc tgcaacggga
    15121 aaaggacaaa aagcccagcg cccgatttgg cagcattaaa tactgagtga caccgcggcg
    15181 taaaccatgt gcgtgcgcgg cttcccataa tggctgtgct tcgctgaata agtcatcatt
    15241 ccacattaaa tggccctgac taaagttttc agggttcagc acaggatcaa ttgcgagaaa
    15301 attttttgcc tgataataac taacccacga ctcagggtaa ttggtgtaga aagccacttt
    15361 aggtcgcgtg aatggtaccg ggtggcgaac acataacgaa tagtaatcgt actccagctg
    15421 ctgagcctga agctctattt catggtaggc ctcttctgcg gcctccatcc tctgaaaacg
    15481 caacagcatc gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc
    15541 ccctgagagt gagtatcatt tataatgata attcaaacta tcacataaat aagatttata
    15601 tataatttat attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt
    15661 attaatgagt tatgctgatt tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc
    15721 cgacaggtga atcgtcgggg ctgatttttt cttattattg cagcagaagc ttctcgagga
    15781 actggcgggt gcgaggctgc tggggatcgg caaataacgc ttttgctgcc ccctgctcga
    15841 ctatccgccc ctggtccata aagatcgccc ggtccgcaac atcccgggca aagctcattt
    15901 cgtgtgtcac aatcaccatc gtgcgctttt cctgcgccag ctggcggatg gtgttcagga
    15961 cttcgcccac cagctctgga tccagcgccg acgttggctc gtcaaacaaa atcacctcag
    16021 gacgcattgc cagtgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac
    16081 gtggatagct ggtttcttta ccggccagcc caactttcgc cagcaactcg cgagcgcgcg
    16141 ccgtggcctc ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttttcca
    16201 gcaccgtacg atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca
    16261 actggcgaat cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc
    16321 caacggtgat cgttcccgct tccggttgtt ccagcagatt tatgctacgt agcaatgtgg
    16381 ttttgccgga accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt
    16441 cgataccgtg cagcaccgtc tgaccgtgga attttttcac caggttctta acttcaatgg
    16501 cactcatttt ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt
    16561 cgataacaca gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac
    16621 ctccagcgtg cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt
    16681 cgcggccagc gacgtatctt ttaccagact gataaagctg ttcgacagcg gcggcaacgc
    16741 cacccgcgcc gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat
    16801 actggcagcg gcttcccact gacctttatc aatagaagaa atggcggcgc gcagcgtttc
    16861 ggcggcatag gcggcagtgt tcagcgacag accaatcatt gctgacggaa tgggatctaa
    16921 ttcgatacca aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt
    16981 accacgaaaa atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag
    17041 gcgcatcagc gcgagaataa acccgagtag taagccaaaa aacatgccgc cgatactgag
    17101 ttgcagggta tacccagccc ctttcaacag gaacggcaag gaatcaataa ccagttgtat
    17161 actttcttgc atgagcgttc ccggagctaa acgtggggat gataggcgaa cagcgcaggc
    17221 gcgccgccgg tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata
    17281 ccgtcaatca gccccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc
    17341 agacgcgcca gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt
    17401 gcaaaatagt catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca
    17461 atcgcctgtt gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc
    17521 acgccaatca gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga
    17581 gttccggcac tgcccgatgc gactaccacc gacgaaatat taaccgcccc ttcacactgt
    17641 tgcgcgattt ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca
    17701 accggaatga catacgggcg aaagccttgt gcttcgactc gcgtcgccag ttcttccagt
    17761 tgggcattgg gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc
    17821 aacaaacgat tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc
    17881 agcgccacgc agtgcagacc gagtttcgcc gcgactgcgg cagtctggcg cacatggtta
    17941 gactggatcg cgccggcagt aatcagcgta tcggcacctt cgcgcagggc atctgccgcg
    18001 agaaattcca gcttacgtaa tttattgccg cccattgcca tgggggtgac ctcatcccgt
    18061 ttgatgaaaa tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt
    18121 ggcgcgccga taaactccaa ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc
    18181 tccgatgtgt gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag
    18241 gcgctagtga aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa
    18301 accatttttc ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat
    18361 tcaccgcttt cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg
    18421 agaatgcttc accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg
    18481 ccagacgatc aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg
    18541 ggtcatcatc ataggtacgc acatcgacac cctgaacatt ctgccgcagc cactcttcat
    18601 agttggtgcc cagaccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg
    18661 tgccttcgtt accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga
    18721 aatcgtattt tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc
    18781 gtttagagtc cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa
    18841 cgccaagatg ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt
    18901 catccccctg aaaactgaac ggcggataag tgccttccag ccctaccagc agcgtgccgc
    18961 tctctttgac tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa
    19021 ccagcgccac ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat
    19081 tcaccccgaa tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa
    19141 caactccgct acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt
    19201 tctgcacaag tccggtacag gtttggcgct gatagtgttg atacttccgt aatgcgatgc
    19261 ggtaattgtt ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt
    19321 cctgcagctg atcgagggaa atattttgct catgtagatg attacccaaa cggcgcagac
    19381 gaacgacata ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaatttct
    19441 taaagccgat aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc
    19501 ggcacaatgc cgcccatgat tgttgttccg tttgccagcc tttttcatca agcagcgtgt
    19561 ccaggcggga aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat
    19621 cgagtaattt acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc
    19681 ggcttaatgg ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt
    19741 taaagtgcgg catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgg
    19801 cctggctgtg taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt
    19861 tgagattcag ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg
    19921 tttcgatggc ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcgggt
    19981 tttctcgctg atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcacgccact
    20041 catcgtagga gaagagctgg ctgttattgg tgtcgagcag catttgacga taatcggcaa
    20101 tatcgatccc taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct
    20161 ccagttgccc tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc
    20221 agtcacggct gcgaagttca tccagcatag cgccacggat acgctacact gcgtaagttg
    20281 taaatgccgt tccttgtagg gcgtcatagc gttcgacagc attaagtaac ccaatgccgc
    20341 ccgcctgtag cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg
    20401 cttcgtgacg caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt
    20461 cagcggtata gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat
    20521 tatccgtttc tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg
    20581 gggttacgca aaaagccgaa tgtaaaaaac cccgccggtg gcggggtttg agaggcattc
    20641 agacgattaa ccctgcagca gagacagaac ctgctgcggt acctggttgg cttttgccag
    20701 cacggagtta ccggcctgct ggataatctg cgctttcgac atgttggaca cttcggtcgc
    20761 atagtcggcg tcctgaatac gggactgcgc ttcagacagg ttggtagtgg tgttgttcag
    20821 gttggtgact gcggaatcca aacggttttg caccgcaccc agggaggagc ggaatttgtc
    20881 gatggagctg atggcttcat ccagagcttt caggggatcg gctgtcgtag ctgcttttgt
    20941 ttcagcatct gtcgtcaatt taccatcagc atctttataa actgccttac cgctgccgtt
    21001 agtcactgaa ccatcatcct gtaggtaaaa atctacagta tcagatgtgg tcaacgcctc
    21061 gttgttatca acataaaccg aatcaacacc ttttgttaca gcgccagttg tgccgttgac
    21121 gctataacct gcagtagtga aatcaacatt gtttcccgag gttgatacag ctttatcgaa
    21181 agctgcctga tcaacttttg cacctgttac tgaataagta atagtgtccg gcgtagtact
    21241 attaccggta aaggagatag aggtaccgcc aatgtcaatg gttgtcgctt tggtattatt
    21301 ggctgcaagt gtggacagac tagcctcagt caaagaagca ccagagccgt ttttcgttaa
    21361 gcgcccagtt gtatcaatgt agagtttatc tccattgctt gccgtaattt taccatcact
    21421 gctgatctga acatcctgtg cagaaccatc gatagtaata gtgcccttag cggtatcacc
    21481 aactcccggg gtcaaatatt tctgaacatc ggcagctgaa gccgttgtgg tatcaaaaga
    21541 gtatgactta cttgcactgt catacttgta attcgttgca ctagccgccg ttccgaagcc
    21601 gttattaatg gttgcctgaa cagtatcacc atcactaact ccagccagca aatcagacgc
    21661 tttagcagca tcgtaacccg cactcacagt atatttgttg ccgaccactg ttgcattagc
    21721 agctaccaag tcagctttag atgcagcagt attagccaca gccccgccac cattcacatt
    21781 aaacccattc agccccagcg tatcagagtc aattttcttc agatcaatag tgatagtctg
    21841 gccgtcattc gcaccaacct gaattttcat cgaaccgtct tttgccagta cgttcacgcc
    21901 gttgaactgg gtctgaccgg atacgcggtc aatttcgtcg agacgggatt tgatttcgtc
    21961 ctgaatggag tccagatccg aatcagagtt agtcccggta gaagcctgaa ccgtcagctc
    22021 acggatacgc tgtaagttgt tgttgatttc ggacagcgcg ccttcagtgg tctgtgcaac
    22081 agaaataccg tcgttggcgt tacgtgcagc ctgagtcagg cctttaatgt tagaagtaaa
    22141 acggttagca atcgcctgac ccgcggcgtc atccttcgcg ctgttaatac gcaagccaga
    22201 agacagacgc tcgatagaac tcgacagcgc agactggttc ttgttgatat tattttgagt
    22261 gatcagcgag aggctgttgg tattaatgac ttgtgccatg attcgttatc ctatattgca
    22321 agtcgttgat tacgttttgg gtttccaccc gtcggctcaa tcgccgtcaa ccctgttatc
    22381 gtctgtcgta aaacaacctt tagaattttt ttcacaaaca gccatttttt gttagtcgac
    22441 gaaatactct tttctctgcc ccttattccc gctattaaaa aaaacaatta aacgtaaact
    22501 ttgcgcaatt caggccgata accccggtat tcgttttacg tgtcgaaaga taaaaggaaa
    22561 tcgcatggca agtatttcat cgctgggagt cgggtcaggt ctggatttaa gttcaatcct
    22621 tgatagcctc accgccgcgc aaaaagcgac gctaacccct atttcaaatc agcaatcgtc
    22681 gtttaccgct aaacttagcg cctacggtac gctgaaaagc gcgctgacga ctttccagac
    22741 cgccaatact gcattgtcta aagccgatct tttttccgcc accagcacca ccagcagcac
    22801 caccgcgttc agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt
    22861 cacccatctg gcgcaggcgc aaaccctgac cacgcgcacc accagagatg atacgaaaac
    22921 ggcgatcgcc accagcgaca gcaaactcac cattcaacaa ggcgacgaca aagatccgat
    22981 taccattgat atcagcgcgg ctaactcatc gttaagcggg atccgtgatg ccatcaacaa
    23041 cgcaaaagcg ggcgtaagtg cgagcatcat taacgtgggt aacggtgaat atcgtctgtc
    23101 agtcacatca aatgacaccg gccttgataa tgcgatgaca ctctcggtca gcggtgatga
    23161 tgcgctacaa agttttatgg gctatgacgc cagtgccagc agcaacggta tggaggtctc
    23221 ggtcgccgcc cagaatgcgc agctgacggt caacaacgtc gccatcgaga acagcagcaa
    23281 cactatcagc gacgcgctgg aaaacatcac tctgaacctg aacgatgtca ccacgggcaa
    23341 ccagacgcta accatcactc aggacacctc caaagtgcaa acggcgatta aagactgggt
    23401 gaatgcctat aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga
    23461 tgcgggagct gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg
    23521 gacgattcag acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa
    23581 aacgctggcg cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc
    23641 cgacaaactc accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg
    23701 cgatggtaaa aaaaccggca tcacaaccac catcggcagc aacctgacca gttggctttc
    23761 gacaacgggc attattaaag ccgctaccga tggcgttagt aaaaccctga ataagttaac
    23821 taaagactac aacgccgcca gcgatcgcat tgatgcgcag gtcgcgcgct acaaagaaca
    23881 atttacccaa ctggacgttt taatgacctc gttaaacagc accagcagct acttaacgca
    23941 gcagttcgaa aacaacagta attccaagta atcattattc atcgggagac aggtcatgta
    24001 cgcggcaaaa ggcacccagg cctatgcaca aattggcgtc gaaagcaccg taatgagcgc
    24061 cagccaacag cagttggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc
    24121 gcgactgttt atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat
    24181 caacatcatt gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaattaac
    24241 ccaaaactta attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg
    24301 caacgatgtc tcagcagtcg aagaggtgga agcattaatg cgtaatattg ccgatgcctg
    24361 gaaagagtct ttactctccc cttcttcgat tcaggaccca gtctgatgaa caatgcaccg
    24421 catttgtatt tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca
    24481 acggaagaac aatgggacga actcatcgct agcgaaatgg cgtatgtgaa tgcggtgcag
    24541 gagatcgcac acctgactga agaggttgcc ccgtccacta cgatgcagga gcagctccgc
    24601 ccgatgctgc acctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg
    24661 atggatgaac tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc
    24721 tatggcgatc agggcggctt tgtgctggct ccgcaggata acctcttttg aatcttaatg
    24781 agccgatggc tcgcgaataa tccgattacg gctacgcttc taatattccc cttgaatgga
    24841 gtcgaagaat gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg
    24901 gtaagctctg gcctgaactg gccgagcgcg cagacggttt taatgatatt ggtatcaata
    24961 tggtctggtt gccgcccgcc tataaaggcg catcgggcgg ctattcggtc ggctacgact
    25021 cctatgattt atttgatcta ggtgagtttg atcagaaagg cagcatccct actaaatatg
    25081 gtgataaagc acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc
    25141 tgctggacgt ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc
    25201 agcgtgtaaa tgctgatgac cgtacgcaaa ttgacgaaga aatcattgag tgtgaaggct
    25261 ggacgcgtta caccttcccc gctcgtgccg ggcaatactc gcagtttatc tgggatttca
    25321 aatgttttag cggtatcgac catatcgaac accctgatga agatggcatt tttaaaattg
    25381 ttaacgacta taccggagaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg
    25441 attatctgat gggtgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat
    25501 actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg ctttcgtctt gatgcggtca
    25561 aacatattcc ggcatggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa
    25621 agccgctgtt tattgtggct gaatgctggt cgcatgaagt cgataaactg caaacgtata
    25681 ttgatcaggt ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg
    25741 aagcatcgcg catgggtcgc gactacgaca tgacgcagat cttcactggg acgctggtgg
    25801 aagccgatcc ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag
    25861 ccctcgaagc accggtcgaa ccgtggttta aaccgctggc gtatgcctta attttgttgc
    25921 gggaaaatgg cgttccttcg gtgttctatc cggacctcta cggtgcacat tacgaagatg
    25981 tcggtggtga cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt
    26041 taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac attatttttc gaccatccga
    26101 actgcattgc ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt
    26161 cgaacggaga tgatggcgaa aaaaccattc atctgggaga gaattacggc aataaaacct
    26221 ggcgtgattt tctggggaac cggcaagaga gcgtagtgac agacgaaaac ggcgaagcga
    26281 ccttcttttg caacggcggc agcgtcagcg tgtgggttat cgaagaggtg atttaaatcc
    26341 atccccggcg gcaagccggg gagatttcat tacggcagtg gcgtcggtaa cggcgcttta
    26401 tccagcgcgg cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg
    26461 tactcaatgg tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta
    26521 gtgagatcat cagacatcaa tgtcaactta cccggcacgg caatcccccg ctgccactga
    26581 cggcaatcca aggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccggactg
    26641 accagcgcac gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg
    26701 gttttcacga cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg
    26761 cctgcaattg cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca
    26821 ttaaagctct gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat
    26881 ttttgtgaat tattaccccg aaaataaatt tgcatggtgt atgattcgcg gctcttgttt
    26941 caaaccttct gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt
    27001 tgattaaatt ctgggcaccc atccctgcgg tcatcgcggc gggtattctc tccacttact
    27061 attttggcat tactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc
    27121 agctcctgca gctgtttggc gtccatgctg aagagtgggg ttattttaaa attatccatc
    27181 tggaaggatc gccattaacc cggatcgacg gcatgatgat cctcggtatg tttggcggct
    27241 gctttgctgc tgcgctgtgg gccaacaatg tcaaactacg catgccacgc agccgtatcc
    27301 gcattatgca ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg
    27361 gctgtaacct ggcggcgttc tttaccggca ttcctcagtt ctcgctgcat gcctggttct
    27421 ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct
    27481 tccgtattcc cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg
    27541 atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg
    27601 cgctgctcac ggcgatgaac caaccaaaac tggggctggc aatgctgttt ggcgtcggct
    27661 ttggtttact gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga
    27721 tcaccggacg tacccatatg gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg
    27781 ggatcttcag ttacgtacag ttaggcgttg aacctaaaat catgtgggcg ggaccaaatg
    27841 cggtaattgg tggtttactt tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg
    27901 gctggatgta ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaacg
    27961 tgatcggctc aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg
    28021 actgggacaa aatcaacctg ctgaaaactt ttggtccgat gggaggcctg ctggtgacat
    28081 atttgctgct gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc
    28141 gtgaggcacc gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct
    28201 tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca
    28261 attaaaaaaa ggtgagatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat
    28321 tccactggat gcgcgtaatc acggctacac ggtgctggat attcagcaag acgggccgac
    28381 cattcgttat ttaattcaga agtaatccct cattcccgct gtggcccaca gcgggaacat
    28441 cgttacctct gacacataga ttttacccgc cccctctcct attcttttca gtatgatctc
    28501 aggaggttgc tatgtgtgga cgctttgccc aatcccaaac gcgtgaggat taccttgcgc
    28561 ttctcgcgga agatattgaa cgcgatattc cctacgatcc cgaacccatt ggcagataca
    28621 atgtcgcgcc gggaaccaaa gttcttctgc tcagtgaacg tgatgaacac cttcatctgg
    28681 atccggtttt ctggggatat gctcccggat ggtgggataa accgccgctg attaacgccc
    28741 gcgtagaaac tgcctcaacc agtcgcatgt ttaaaccgct ctggcaacat ggtcgggcaa
    28801 tctgttttgc cgatggctgg tttgagtgga aaaaagaagg cgacaaaaaa agccttattt
    28861 tatctatcgc gctgacggac aacctgtttt tatggccgcg ataggtagca taccgtttga
    28921 gcgtggtgac gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt
    28981 agatattcat gaccgccgcc cgctggttct gtcgccagaa gctgcacggg aatggatgcg
    29041 gcaagacatt ggtggtaaag aagcctcaga aatcgctacc agaagctgtg tgccagcaaa
    29101 ccagttcacc tggcaccctg tgtcgcgcac ggtgggtaat gtcaaaaacc agggcgcgga
    29161 gttaattcaa cctgtttgtt gacatcctcc acgccctgaa tgacgaggac ccctgctacg
    29221 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca
    29281 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gctccattta
    29341 catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcgat
    29401 tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag
    29461 caagcacctg accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc
    29521 agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct
    29581 ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta
    29641 tcatt
//
LOCUS       DAJTNP010000043        29588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTNP010000043 DAJTNP010000000
VERSION     DAJTNP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            46..840
                     /locus_tag="OO160_002098"
     CDS             46..840
                     /locus_tag="OO160_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5524218.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            862..2271
                     /locus_tag="OO160_002099"
     CDS             862..2271
                     /locus_tag="OO160_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524219.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2249..3028
                     /locus_tag="OO160_002100"
     CDS             2249..3028
                     /locus_tag="OO160_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5524220.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3025..3885
                     /locus_tag="OO160_002101"
     CDS             3025..3885
                     /locus_tag="OO160_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5524221.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4033..4608)
                     /locus_tag="OO160_002102"
                     /pseudo
     CDS             complement(4033..4608)
                     /locus_tag="OO160_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4625..4885)
                     /locus_tag="OO160_002103"
     CDS             complement(4625..4885)
                     /locus_tag="OO160_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5524222.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4876..6147)
                     /locus_tag="OO160_002104"
     CDS             complement(4876..6147)
                     /locus_tag="OO160_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5524223.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6225..6587)
                     /gene="queD"
                     /locus_tag="OO160_002105"
     CDS             complement(6225..6587)
                     /gene="queD"
                     /locus_tag="OO160_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5524224.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6906..8705
                     /gene="cysJ"
                     /locus_tag="OO160_002106"
     CDS             6906..8705
                     /gene="cysJ"
                     /locus_tag="OO160_002106"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5524225.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8705..10417
                     /gene="cysI"
                     /locus_tag="OO160_002107"
     CDS             8705..10417
                     /gene="cysI"
                     /locus_tag="OO160_002107"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5524226.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10491..11225
                     /gene="cysH"
                     /locus_tag="OO160_002108"
     CDS             10491..11225
                     /gene="cysH"
                     /locus_tag="OO160_002108"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5524227.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11489..11641
                     /locus_tag="OO160_002109"
     CDS             11489..11641
                     /locus_tag="OO160_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5524228.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11835..12926
                     /locus_tag="OO160_002110"
                     /pseudo
     CDS             11835..12926
                     /locus_tag="OO160_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13072..14109)
                     /gene="iap"
                     /locus_tag="OO160_002111"
     CDS             complement(13072..14109)
                     /gene="iap"
                     /locus_tag="OO160_002111"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5524229.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14361..15269
                     /gene="cysD"
                     /locus_tag="OO160_002112"
     CDS             14361..15269
                     /gene="cysD"
                     /locus_tag="OO160_002112"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5524230.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15271..16698
                     /gene="cysN"
                     /locus_tag="OO160_002113"
     CDS             15271..16698
                     /gene="cysN"
                     /locus_tag="OO160_002113"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5524231.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16698..17303
                     /gene="cysC"
                     /locus_tag="OO160_002114"
     CDS             16698..17303
                     /gene="cysC"
                     /locus_tag="OO160_002114"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5524232.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17353..17676
                     /locus_tag="OO160_002115"
     CDS             17353..17676
                     /locus_tag="OO160_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5524233.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17870..18181
                     /gene="ftsB"
                     /locus_tag="OO160_002116"
     CDS             17870..18181
                     /gene="ftsB"
                     /locus_tag="OO160_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5524234.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18200..18910
                     /gene="ispD"
                     /locus_tag="OO160_002117"
     CDS             18200..18910
                     /gene="ispD"
                     /locus_tag="OO160_002117"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5524235.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18910..19389
                     /gene="ispF"
                     /locus_tag="OO160_002118"
     CDS             18910..19389
                     /gene="ispF"
                     /locus_tag="OO160_002118"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5524236.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19386..20435
                     /gene="truD"
                     /locus_tag="OO160_002119"
     CDS             19386..20435
                     /gene="truD"
                     /locus_tag="OO160_002119"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5524237.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20416..21177
                     /gene="surE"
                     /locus_tag="OO160_002120"
     CDS             20416..21177
                     /gene="surE"
                     /locus_tag="OO160_002120"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5524238.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21171..21797
                     /gene="pcm"
                     /locus_tag="OO160_002121"
     CDS             21171..21797
                     /gene="pcm"
                     /locus_tag="OO160_002121"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5524239.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21937..23076
                     /gene="nlpD"
                     /locus_tag="OO160_002122"
     CDS             21937..23076
                     /gene="nlpD"
                     /locus_tag="OO160_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5524240.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23139..24131
                     /gene="rpoS"
                     /locus_tag="OO160_002123"
     CDS             23139..24131
                     /gene="rpoS"
                     /locus_tag="OO160_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5524241.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24251..24658)
                     /locus_tag="OO160_002124"
     CDS             complement(24251..24658)
                     /locus_tag="OO160_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5524242.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24805..25398
                     /locus_tag="OO160_002125"
     CDS             24805..25398
                     /locus_tag="OO160_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5524243.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25398..26345
                     /locus_tag="OO160_002126"
                     /pseudo
     CDS             25398..26345
                     /locus_tag="OO160_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26618..27181)
                     /locus_tag="OO160_002127"
     CDS             complement(26618..27181)
                     /locus_tag="OO160_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524244.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27346..27597
                     /locus_tag="OO160_002128"
     CDS             27346..27597
                     /locus_tag="OO160_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5524245.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27599..>29588
                     /locus_tag="OO160_002129"
     CDS             27599..>29588
                     /locus_tag="OO160_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5524246.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAV"
BASE COUNT         7216 a         7349 c         8010 g         7013 t
ORIGIN      
        1 atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa aatctttaaa
       61 tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa aaccggcttc
      121 aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg tttgtatgac
      181 gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt gctgatcttt
      241 atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc ggaaatcgtt
      301 gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg gtttaacaac
      361 ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa aaccggcaac
      421 atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat ctacgaaagc
      481 gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat gctgggcggt
      541 cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga ctacaacgtt
      601 gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa caaaatcatc
      661 tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat tggtaaacat
      721 cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga agggctgaaa
      781 aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat tgaaaaataa
      841 tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc aggcgagaag
      901 ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt gcgaatggat
      961 gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg cgcacacctg
     1021 accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac gcccgccatg
     1081 caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg tttgtttctt
     1141 ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat cttcaccttc
     1201 agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac gccagagcat
     1261 cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc agtaggtcac
     1321 accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg cgcattcagc
     1381 gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt tatttccgaa
     1441 aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt gttgattacg
     1501 ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg gcgttttgca
     1561 gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg cgatgaagtg
     1621 gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg gcggcgcacg
     1681 gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat ttatacctgg
     1741 ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag cctgatgctt
     1801 aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct gctggcacat
     1861 cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt aatggcctgt
     1921 ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac caccatttcg
     1981 gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga cattcgctcg
     2041 ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag tactggcctg
     2101 ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct agcgacagtg
     2161 ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc cctcccgctg
     2221 gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta aagccgaacc
     2281 ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta atagcggacc
     2341 ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg ccgcgctgct
     2401 gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca tgggggatga
     2461 acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag ctgtattgct
     2521 ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata ttgccgaatg
     2581 gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg aagggcagaa
     2641 tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca cccaggtgga
     2701 gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac atgggttgcg
     2761 ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg aagtggcctt
     2821 gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta tccgtaaaac
     2881 tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag agcaacgacg
     2941 cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc tatggcagga
     3001 ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa tcaggagaat
     3061 gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct ggcgcactgg
     3121 caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt ggaacagtgg
     3181 cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga tgaactgtcg
     3241 acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc gttagatatt
     3301 ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac gctgcaaaca
     3361 gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa aaacgccacg
     3421 ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga ctggctggtt
     3481 agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag acgggtgctg
     3541 gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc agaaaagctg
     3601 ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga tgctgaaaaa
     3661 gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt tggcgcttcc
     3721 ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc gattaatcat
     3781 gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga ctggaaagtg
     3841 gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc tgccggatga
     3901 tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt aagcgtcgca
     3961 tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat tcaattggtt
     4021 acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat tcgtggtgga
     4081 aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc ataccagccc
     4141 gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt tgggcatgca
     4201 gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat caatgttgtg
     4261 aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt ccgctcttgc
     4321 cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca ccagtttcaa
     4381 aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat gaataaaagc
     4441 atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga tattgccgta
     4501 caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg cattatcttt
     4561 aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa attatcctta
     4621 ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc cactgttgta
     4681 gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg cgatagtcaa
     4741 tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg ttaatcaacc
     4801 gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca tcagcaacgc
     4861 gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg ccagatgcca
     4921 caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa caggtttatc
     4981 accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg cccgatacct
     5041 gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat cccatagcag
     5101 gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc ggtgctggca
     5161 ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat ccatgccgcg
     5221 cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca gccagccgtt
     5281 accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat gcgcaccata
     5341 ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt taaagcgagt
     5401 cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc aaacaatccc
     5461 taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt catcacagat
     5521 tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac gttcttcaat
     5581 agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt cgccagcagg
     5641 acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg ccagcaccac
     5701 ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc cgttttcttc
     5761 atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt cggcttcggc
     5821 aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc cgggctgtaa
     5881 gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt gagtgatgcg
     5941 tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg catgggtata
     6001 taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga gtaacaaaac
     6061 ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac cggcaccaat
     6121 aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac acaacaccct
     6181 aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc gccgcgatag
     6241 atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg cacaacgggt
     6301 ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc cagacctgga
     6361 atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc cgctttcagt
     6421 tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat ttccagtcgc
     6481 accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac atgtggtaag
     6541 cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat aatttctcta
     6601 caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt atctaaaacg
     6661 actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat ttgtttttca
     6721 ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt agtcgatttg
     6781 gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta accttacctt
     6841 ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact ggaacataac
     6901 gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg gagcaactgg
     6961 cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg gtttctggct
     7021 atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca gcgcccgccg
     7081 cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg cgccgggttg
     7141 ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg gtgaacgcgg
     7201 gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg acgtcaacgc
     7261 aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg ttctccaaaa
     7321 aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat agctcttatg
     7381 aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg gggggtgaac
     7441 gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc gagtggcgcg
     7501 cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg caatccgtcg
     7561 ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg ccgctggtgg
     7621 ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac gttcgccata
     7681 tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg ctgggcgtct
     7741 ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg ctgacaggcg
     7801 atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta cagtggcact
     7861 tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt acccgcagtg
     7921 aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc gcgacgacgc
     7981 tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg ctaattaatc
     8041 tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg gaagtcgaga
     8101 acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc gcccgtgccg
     8161 gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc cgcgtattta
     8221 tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg attatgattg
     8281 gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc gctgacgaag
     8341 cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac ttcctgtatc
     8401 aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat ctcgcctggt
     8461 cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag ggcgcggagc
     8521 tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct aatcgcatgg
     8581 cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc atggacaccg
     8641 aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag cgagatgtct
     8701 actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga cagacgccga
     8761 gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt taaacgacgg
     8821 tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg gcatgtatca
     8881 gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc cgcgccacgc
     8941 gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt ggcaggcgat
     9001 cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca accgccagac
     9061 gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga tgctgcactc
     9121 ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac tctgcacctc
     9181 gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga agatttctga
     9241 acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg aaaaagtcgc
     9301 caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt tcaaaaccac
     9361 ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga acttcgtggc
     9421 gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg ggctttctat
     9481 cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct atctgccgct
     9541 agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact ggggtaaccg
     9601 aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg ttgagacgtt
     9661 taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc cgtatgaatt
     9721 tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact ggcacctgac
     9781 gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga aaactggcct
     9841 gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc agaatctgat
     9901 catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca aagagagcgg
     9961 gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt cattcccgac
    10021 ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg acaacatcga
    10081 taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg taacaggctg
    10141 cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta aagcaccggg
    10201 tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac ggatgtataa
    10261 agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag ggcgctgggc
    10321 gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg gcatcattcg
    10381 cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt cttgtaggcc
    10441 tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct atgtccaaac
    10501 tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg ctggcagaaa
    10561 ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg ctggataatc
    10621 tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg agcctgcatc
    10681 tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc tatctgttcc
    10741 cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac ctgaaagtgt
    10801 accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg tgggaacagg
    10861 gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg aaccgggcgc
    10921 tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa tccggcagcc
    10981 gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg ccgattatcg
    11041 actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa tatcacccat
    11101 tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa tgggaacccg
    11161 gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga ttacacgaag
    11221 ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc gtttttattt
    11281 tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac aggtatagaa
    11341 tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca gatggacaaa
    11401 agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca attcggatgt
    11461 tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg tggcaatcat
    11521 cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt tatgtgagtt
    11581 gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat cgaagaagta
    11641 gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc tcctgcaccc
    11701 tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat tatgaatttc
    11761 aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata tatatcatag
    11821 atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa aatattgtga
    11881 ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc gacgactatc
    11941 accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg gttaaaagaa
    12001 attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag gaacaggcag
    12061 ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc cgctcttttc
    12121 aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa aattacgaaa
    12181 aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt gaatgtcagg
    12241 agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata gaaatgtgga
    12301 tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa ttggataatt
    12361 ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag ccactgtttc
    12421 cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt ataaaacaac
    12481 tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg tcaacgcgat
    12541 tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag gctttagttc
    12601 aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct ttcaccggaa
    12661 ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag gtgctggatc
    12721 tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc ggcgcaggta
    12781 aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac gctcaggggc
    12841 tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga ctggtcaaaa
    12901 cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata aaccggcgca
    12961 atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat gggtatcact
    13021 tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc atcaagacgc
    13081 cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc agcgacgttc
    13141 tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta cgtcatgcca
    13201 gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat ccttatttcc
    13261 aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt cgaatatttc
    13321 tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg gattggtcgt
    13381 tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta atttccttac
    13441 tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa ttaagttatc
    13501 gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct tgagtaaatt
    13561 ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa taccatactc
    13621 ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac ctgcggcgtt
    13681 atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag catcgctcag
    13741 cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt tgccttcatg
    13801 agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat cgcgggcggt
    13861 ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt gctgaaactg
    13921 ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc gtcccggaaa
    13981 gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag gtgacgaggc
    14041 gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt ggcgcaatgc
    14101 ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata gtatgaaact
    14161 gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt gccttaagca
    14221 ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca ttctaattgg
    14281 taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc
    14341 ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg caactggagg
    14401 cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg gtgatgctct
    14461 actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg ttttatccag
    14521 gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc gagatgtatg
    14581 agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat aaaaacccgg
    14641 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta
    14701 tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg
    14761 gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct ttccgtgacc
    14821 gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac tacaacgggc
    14881 aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc gagcaggata
    14941 tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc gctgcggaac
    15001 gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc
    15061 aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc
    15121 tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg
    15181 tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg ggatccatgg
    15241 agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca acaaatcgcc
    15301 aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct gctgcgtttt
    15361 ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct gctgcacgat
    15421 acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa gcgtcacggc
    15481 acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc tgagcgcgaa
    15541 cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg taaatttatt
    15601 atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg cgcatcgaca
    15661 tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca aacccgtcgt
    15721 cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat caacaaaatg
    15781 gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta tctgaccttt
    15841 gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc actggaaggt
    15901 gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac actgctcgaa
    15961 gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg cttcccggtg
    16021 cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct ggcatccggt
    16081 cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc aaacgtcgcg
    16141 cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc gatcaccctg
    16201 gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc agacgaagcg
    16261 ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca gccgctttcc
    16321 ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg tgttgatggc
    16381 attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa cctgccgctg
    16441 aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga tcgctatcag
    16501 caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt gaccgtgggt
    16561 gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga attcagtgca
    16621 ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg cgcgcgcgat
    16681 ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat agtcatccgg
    16741 tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg tggtttaccg
    16801 gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg ttacataaac
    16861 tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta tgcagcgatc
    16921 tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa gtggcgaatt
    16981 tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac cgcgccgaac
    17041 gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt gtcgatacgc
    17101 cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg cgtgccggtg
    17161 aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg gcagaaattc
    17221 atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat ctgttgagac
    17281 agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt cgtcaaagta
    17341 acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga cagccttact
    17401 gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat ctcctggtta
    17461 tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt ctttctctac
    17521 acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat tgcgctgcat
    17581 tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac agtaggcatt
    17641 atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg tctcctctgt
    17701 tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg gtatacgtaa
    17761 caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca gaggatgtag
    17821 agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga tgggtaaact
    17881 aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt tcggtaagaa
    17941 cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag ctacaaacgc
    18001 gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca atggcggcca
    18061 ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg gcgaaacttt
    18121 ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa acaatcgata
    18181 aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg tggttccggc
    18241 ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct caatcggtaa
    18301 tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg tgacacgtgt
    18361 cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg cgaatcatcc
    18421 gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg caggtctgaa
    18481 agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt gtttgcatca
    18541 ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag ggatcctcgc
    18601 cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca ttgctcatac
    18661 cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc gtgagctgtt
    18721 acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg aagcctcggc
    18781 gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata acattaaagt
    18841 cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca tccatcagga
    18901 gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg tgaaggccca
    18961 attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca ttctgatggc
    19021 gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct gggggatatc
    19081 ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg cgagctgcta
    19141 cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt cgatgtcact
    19201 atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt gtttattgcc
    19261 gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac ggaaaaactt
    19321 ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact cattaaggca
    19381 acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa ggcaccgggc
    19441 tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt gagcctgatg
    19501 gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc cgttttgtgg
    19561 cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc gctgggcaaa
    19621 aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc aaggaaatgc
    19681 ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg cggcacaagc
    19741 gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg cgcgaagtga
    19801 gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt gtaccgaact
    19861 acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg ctgcgctggg
    19921 cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg tcggcagccc
    19981 gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac gttaatcaag
    20041 ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta gcaaccaccg
    20101 aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata accgccgcat
    20161 tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa caagcagctg
    20221 tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa gccgcgcgca
    20281 gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac gtcaccgtag
    20341 agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg gaacttatca
    20401 acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca tgcacccggt
    20461 atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt cgcccccgat
    20521 cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg cacgtttacc
    20581 tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt ctatcttggc
    20641 gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa cgccgggccg
    20701 aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga aggccgtcat
    20761 ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga cactgccgcg
    20821 gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac cggacgtatt
    20881 cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg cgtgacgcgc
    20941 tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg cggcaatacg
    21001 ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac cgattttgct
    21061 gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac tgcgcatagc
    21121 gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca atggtaagca
    21181 gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat gagcaggtgc
    21241 tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt gaacaaaaag
    21301 cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag ccgtatatgg
    21361 tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg gaaattggca
    21421 ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt tgctcggttg
    21481 aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt gatttacata
    21541 atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg ccgtttgacg
    21601 ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg cagctggacg
    21661 aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa cgggtgcgtc
    21721 gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc cctttagtga
    21781 agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta ttttgccgta
    21841 ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca cggtaattat
    21901 gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag cccaaaattc
    21961 accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg ctgttctgac
    22021 acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc aaatactaat
    22081 tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc gcagcaaccg
    22141 caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca aatccagcca
    22201 gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt ctataaccgt
    22261 cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc
    22321 gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc
    22381 aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt gggtaatgct
    22441 tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga
    22501 gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg
    22561 tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccaact
    22621 gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccgactgtc
    22681 agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg
    22741 atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa
    22801 ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa cgcgctgcgc
    22861 ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat
    22921 aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc
    22981 atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa
    23041 tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt
    23101 gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag
    23161 ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa
    23221 aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc
    23281 agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag attggttatt
    23341 caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg
    23401 tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc
    23461 gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac ttggggctga
    23521 tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca tacgcaacct
    23581 ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact attcgtttgc
    23641 cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag ttgtcccata
    23701 agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag ccagttgatg
    23761 acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc ccgctgggtg
    23821 gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac ggtccggaag
    23881 ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc gagctgaacg
    23941 ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa gcggcaacac
    24001 tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag attcaggttg
    24061 aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc gaagcgctgt
    24121 tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg gcctttttct
    24181 tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt cctacccggc
    24241 aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc tcctgctctt
    24301 ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt atcgccgcag
    24361 caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg tcagcgacgt
    24421 catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct gctagcgttg
    24481 ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt ttatcagcaa
    24541 tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc tgccagcgag
    24601 ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt aacgccatgt
    24661 aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga agaagatgtt
    24721 gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat aacgttcgca
    24781 tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg ggctaccggt
    24841 gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt cgagactcat
    24901 ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta cagcgctcgc
    24961 gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc gatcatctca
    25021 tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa aacgctcgcc
    25081 ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt cgtgctcaaa
    25141 gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat ccatctcgaa
    25201 aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc tgccttttat
    25261 aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt gctggatcaa
    25321 tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc ccggaatttt
    25381 tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag gcgcttgatg
    25441 accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat ctggcagcag
    25501 cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt gataatattc
    25561 gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag aaccacgcga
    25621 tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag tttatccgcc
    25681 gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg gcgcagaaca
    25741 ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc ctgaatgatg
    25801 gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg ctcgacccgg
    25861 ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc aagcgtaagc
    25921 tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa gcagaagagc
    25981 gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc acgctgatgg
    26041 gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg ctgcgtgaaa
    26101 gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg ggttcagaag
    26161 tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg ttcggtgagt
    26221 ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat aaagtctctt
    26281 atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc gaaatcgact
    26341 acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag acgggaatag
    26401 attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc aaaaatgtta
    26461 aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa tagcctttaa
    26521 agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa gcgccaaaac
    26581 caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct gctttctgag
    26641 gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta accttttcgt
    26701 ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga gcgtgcaact
    26761 cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc ccaagaacat
    26821 cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga atttgagcat
    26881 caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca acatcaccct
    26941 ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt tttaactgat
    27001 acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa gtgccttcat
    27061 tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact aacctaaaga
    27121 ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt gcttttctca
    27181 ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa taaaccataa
    27241 aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc catacggata
    27301 tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag acaaagagaa
    27361 gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga taacgggtgg
    27421 tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact gaaagcggta
    27481 gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg tgtaccgcca
    27541 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc gtcgtgatat
    27601 gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag ccaccacaaa
    27661 aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta gtcgccgtcg
    27721 ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa cacgaaaggc
    27781 actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt ctgaagtgaa
    27841 agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag catatcgcgg
    27901 ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc gctgggtatc
    27961 tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg gtaaccccgg
    28021 gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc tgccggagcg
    28081 cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta ctatcagccg
    28141 cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg ccgaacggct
    28201 gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca gctggctgcc
    28261 tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag cctacgagga
    28321 ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg aggtgatgcc
    28381 ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga aacaaaaagg
    28441 gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg actggacagc
    28501 tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga aatgtgcgca
    28561 tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg gtggaacacg
    28621 gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg ccggtgcgat
    28681 acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata acggttccgg
    28741 tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc tggggattaa
    28801 acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac gcctgaaccg
    28861 gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg gtgctgaccg
    28921 ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc tgcaacagga
    28981 caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt gggcggagct
    29041 tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt ctgagctgcc
    29101 gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac gccaggcaga
    29161 agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt tccgcccaat
    29221 ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt actacgcgcc
    29281 ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc atgacgccga
    29341 gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat ggaacggcaa
    29401 taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc gtattaaagg
    29461 aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta ttcagattct
    29521 ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg gtaatgtggt
    29581 ggcagttc
//
LOCUS       DAJTNP010000044        29045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTNP010000044 DAJTNP010000000
VERSION     DAJTNP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO160_002130"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO160_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5524247.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO160_002131"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO160_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5524248.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO160_002132"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO160_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5524249.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO160_002133"
     CDS             4843..5082
                     /locus_tag="OO160_002133"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524250.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO160_002134"
     CDS             5092..5262
                     /locus_tag="OO160_002134"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5524251.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO160_002135"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO160_002135"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5524252.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO160_002136"
     CDS             6724..6990
                     /locus_tag="OO160_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5524253.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO160_002137"
     CDS             7084..7773
                     /locus_tag="OO160_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5524254.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO160_002138"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO160_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5524255.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO160_002139"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO160_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5524256.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO160_002140"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO160_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5524257.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO160_002141"
     CDS             complement(10475..12193)
                     /locus_tag="OO160_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5524258.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO160_002142"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO160_002142"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5524259.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO160_002143"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO160_002143"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5524260.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO160_002144"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO160_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5524261.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO160_002145"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO160_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5524262.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO160_002146"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO160_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO160_002147"
     CDS             18432..18746
                     /locus_tag="OO160_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5524263.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO160_002148"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO160_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5524264.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO160_002149"
     CDS             20570..21862
                     /locus_tag="OO160_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5524265.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO160_002150"
     CDS             complement(22016..23665)
                     /locus_tag="OO160_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5524266.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO160_002151"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO160_002151"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5524267.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO160_002152"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO160_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5524268.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO160_002153"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO160_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5524269.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO160_002154"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO160_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO160_002155"
     CDS             28753..29034
                     /locus_tag="OO160_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5524270.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7283 a         7777 c         7354 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atacccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaagc
//
LOCUS       DAJTNP010000045        28941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTNP010000045 DAJTNP010000000
VERSION     DAJTNP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..869
                     /locus_tag="OO160_002156"
                     /pseudo
     CDS             1..869
                     /locus_tag="OO160_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1262..1603)
                     /locus_tag="OO160_002157"
     CDS             complement(1262..1603)
                     /locus_tag="OO160_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5524271.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1616..2488)
                     /gene="copD"
                     /locus_tag="OO160_002158"
                     /pseudo
     CDS             complement(1616..2488)
                     /gene="copD"
                     /locus_tag="OO160_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(2492..2866)
                     /gene="yobA"
                     /locus_tag="OO160_002159"
     CDS             complement(2492..2866)
                     /gene="yobA"
                     /locus_tag="OO160_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5524272.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3005..3235
                     /gene="holE"
                     /locus_tag="OO160_002160"
     CDS             3005..3235
                     /gene="holE"
                     /locus_tag="OO160_002160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5524273.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3337..3993
                     /locus_tag="OO160_002161"
                     /pseudo
     CDS             3337..3993
                     /locus_tag="OO160_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4017..4679
                     /gene="exoX"
                     /locus_tag="OO160_002162"
     CDS             4017..4679
                     /gene="exoX"
                     /locus_tag="OO160_002162"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5524274.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4676..6736)
                     /gene="ptrB"
                     /locus_tag="OO160_002163"
     CDS             complement(4676..6736)
                     /gene="ptrB"
                     /locus_tag="OO160_002163"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5524275.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6946..7605)
                     /locus_tag="OO160_002164"
     CDS             complement(6946..7605)
                     /locus_tag="OO160_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5524276.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(7932..8288)
                     /locus_tag="OO160_002165"
     CDS             complement(7932..8288)
                     /locus_tag="OO160_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5524277.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8355..8645)
                     /gene="yebG"
                     /locus_tag="OO160_002166"
                     /pseudo
     CDS             complement(8355..8645)
                     /gene="yebG"
                     /locus_tag="OO160_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            8779..9957
                     /gene="purT"
                     /locus_tag="OO160_002167"
     CDS             8779..9957
                     /gene="purT"
                     /locus_tag="OO160_002167"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5524278.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10013..10654)
                     /gene="kdgA"
                     /locus_tag="OO160_002168"
                     /gene_synonym="eda"
     CDS             complement(10013..10654)
                     /gene="kdgA"
                     /locus_tag="OO160_002168"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5524279.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10691..12502)
                     /gene="edd"
                     /locus_tag="OO160_002169"
     CDS             complement(10691..12502)
                     /gene="edd"
                     /locus_tag="OO160_002169"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5524280.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12737..14212)
                     /gene="zwf"
                     /locus_tag="OO160_002170"
     CDS             complement(12737..14212)
                     /gene="zwf"
                     /locus_tag="OO160_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5524281.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14550..15419
                     /gene="hexR"
                     /locus_tag="OO160_002171"
                     /gene_synonym="yebK"
     CDS             14550..15419
                     /gene="hexR"
                     /locus_tag="OO160_002171"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5524282.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15547..16989
                     /gene="pyk"
                     /locus_tag="OO160_002172"
     CDS             15547..16989
                     /gene="pyk"
                     /locus_tag="OO160_002172"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5524283.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17120..18091)
                     /gene="lpxM"
                     /locus_tag="OO160_002173"
                     /gene_synonym="msbB"
     CDS             complement(17120..18091)
                     /gene="lpxM"
                     /locus_tag="OO160_002173"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5524284.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18211..19533)
                     /gene="mepM"
                     /locus_tag="OO160_002174"
     CDS             complement(18211..19533)
                     /gene="mepM"
                     /locus_tag="OO160_002174"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5524285.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(19549..20481)
                     /gene="znuA"
                     /locus_tag="OO160_002175"
     CDS             complement(19549..20481)
                     /gene="znuA"
                     /locus_tag="OO160_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5524286.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            20560..21315
                     /gene="znuC"
                     /locus_tag="OO160_002176"
     CDS             20560..21315
                     /gene="znuC"
                     /locus_tag="OO160_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5524287.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21312..22097
                     /gene="znuB"
                     /locus_tag="OO160_002177"
     CDS             21312..22097
                     /gene="znuB"
                     /locus_tag="OO160_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5524288.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22244..23254)
                     /gene="ruvB"
                     /locus_tag="OO160_002178"
     CDS             complement(22244..23254)
                     /gene="ruvB"
                     /locus_tag="OO160_002178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5524289.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23263..23874)
                     /gene="ruvA"
                     /locus_tag="OO160_002179"
     CDS             complement(23263..23874)
                     /gene="ruvA"
                     /locus_tag="OO160_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5524290.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24013..24078)
                     /gene="yobI"
                     /locus_tag="OO160_002180"
     CDS             complement(24013..24078)
                     /gene="yobI"
                     /locus_tag="OO160_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5524291.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24148..24750
                     /locus_tag="OO160_002181"
     CDS             24148..24750
                     /locus_tag="OO160_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5524292.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(24752..25273)
                     /gene="ruvC"
                     /locus_tag="OO160_002182"
     CDS             complement(24752..25273)
                     /gene="ruvC"
                     /locus_tag="OO160_002182"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5524293.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25308..26048)
                     /locus_tag="OO160_002183"
     CDS             complement(25308..26048)
                     /locus_tag="OO160_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5524294.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26077..26529)
                     /gene="nudB"
                     /locus_tag="OO160_002184"
     CDS             complement(26077..26529)
                     /gene="nudB"
                     /locus_tag="OO160_002184"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5524295.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(26647..28419)
                     /gene="aspS"
                     /locus_tag="OO160_002185"
     CDS             complement(26647..28419)
                     /gene="aspS"
                     /locus_tag="OO160_002185"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5524296.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            28729..>28941
                     /locus_tag="OO160_002186"
     CDS             28729..>28941
                     /locus_tag="OO160_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5524297.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7035 a         7459 c         7125 g         7322 t
ORIGIN      
        1 ggtcgactca tttactatgg caggctggat tgaacgttac aaaacgattc ttataaggag
       61 aaaagcgtcc agaaatactt ataaaactcg ggtaaatcaa ctggagacaa taaaagaaaa
      121 attaggaggg gttttactga cagaaataac cactcgccat atcgccgagt ttcttgattt
      181 gtagattgaa ggagggaaaa acacgatggc aggatcaatg cgttctgtgt tatctgatat
      241 gttccgcgaa gccattgttg aaggacgtat atctcaaaat ccagtaacgc caacaagagc
      301 accgaaaata gtagttacaa gagaacggct gaaactaaag acatacaact gcatcaggga
      361 ggcagcagat caacttccgg catggttccc attagctatg gacttagccc ttgtaacagg
      421 acaacgtcgc gaagacataa cgaatatgcg gttcagtgat atttatgatg atcgtctcca
      481 catcaggcaa attaagacag gaatgatgat tgctatcccc ctgtcactca gccttcctgt
      541 cgctggttta cgacttggtg cagtagttga acagtgccgc atggtaagta ggggggatta
      601 tctaatcagt gccgggatta gaaaaaacag ccctgacggc agcattcacc cggacggcct
      661 gacaaagaaa tttgtcgcag ccagaaaatt aacaggtatc actgttcagt gaaaacccac
      721 caacttttca cgaaatcaga agcctggctg gacgattgta caaagaaata tgtggagaag
      781 aatttgctca gcgtctactt ggccacacat cggagaagac aacaaaaatg tatcttgatg
      841 agagagaaaa aacgtactta ctgctctgat tttaacgtaa atggattgtt aaatgtattt
      901 tggttgtgat ataaccaaaa aagaccggaa tacagaaatt cgagtaaatt tcggggaatt
      961 tcggggagac gtttgcaact aattgatttt aaatacaatt aaaaaaagac cgaatacgat
     1021 tcctgtattc ggtccaggga aatggctctt gggagagagc cgtgcgctaa aagttggcat
     1081 taatgcaggc ttagttgcct tgccctttaa gaatagatga cgacgccagg ttttccagtt
     1141 tgcgtgcaaa atggtcaata aaaagcgcgg tgctcatcag cttaaatgtt aaaaaccgcc
     1201 cgttctggtg aaagaactga ggcggttttt ttattggaaa tcaaaaggct attttaggta
     1261 attaacagag tttttcagct cgttctataa acggtgccag actcattttt tcgccgggat
     1321 tgttaggatc atcaatctga atcaccgaaa tgggttgggc attggtcttc ccactggcaa
     1381 cttccttttg tgcgatatcg tttaaaggat actgcacgag ggtacttggg ttgatgacat
     1441 acaaagcatt acccggtcgg caagtcagca ttacctcttc gcgattaaac gcccatttgt
     1501 ctttacccac ttcaaaacga ctcacggtta tcacctgtgg agccgccagc gccgctgcag
     1561 aactggtgag taacagaaac gccagaatac tttttttcat cattttcgta atccatcaaa
     1621 aaggttcgag agtcgcaagc aggctgatga tcaccagcac taccgcacct atcgcccatt
     1681 cgagtttagt catccacaca aaccagggtg ccgcacgatc ttcatcctgc ctcatgcgcg
     1741 gtacaagaac ataacgatta gccagcgcaa tgaccaccat aatcatcacc aggatggctt
     1801 tgagcaataa gagttggccc cagtaagtcg taagcgtggg gggaaatccg gtaatcaaca
     1861 atgcattaag cacgccgctt gccagtacgc cgatcaccgc aaaatgcccg caccaggaaa
     1921 aacgcatcag cgcctgaata gcctagtgtc gccaacggcc tttgatgagt tgcatacacc
     1981 agagcaccgg cagtaatccc ccaaaccacg ccgccgcaca aatcaggtga atcgcgtgat
     2041 tagtctgatg gattttcgct gttacccctt cattcagcgt cgcatacccc actcccgcca
     2101 gcagaataaa ttgcgcggta gtgagcataa acagcaaacg tggcatattc cgcggttgca
     2161 taagggcgac gatcaacgtt acgagggcga gaacaatttg ccataaccag acgccaccaa
     2221 actgcgtttg caaaacagcc tgccaaatgt ttggcgaaaa tacatctgtc catcccgttc
     2281 ccatcaaccc gccctgaacg gcgagcattg cagtggcact gatcaaactc cagacggcag
     2341 cgtgctgttg taaacgcaga gaacgcttcg tcagcaagcg acgaatcatt aagggggcca
     2401 gccaggcacc atacattgca aagccaaaga ccagcatcaa cgaggtgaaa tggataaatc
     2461 gcagcgcgat ccaggtaaac gccagcatga tttatttcac gctaaaggtg tagtgtcctt
     2521 tcgttttatg cccgtccacc gaaacaacat gccagtcaac ggtgtaggtt ccgggtttca
     2581 gcgaatcggc cagtggaaca atcagttgct tttggtcctg ctcattccgc ttcgctggta
     2641 atgttttaat attttcgttt tttggccccg tgatttttgc accactgaat cctgtttcaa
     2701 cgccttccga gaagtttaaa gtgattgcct gcggggcagc tgtcacttgc gcgtttgccg
     2761 caggatactg atgcgttaaa tgcgcatgtg cccagacgga aggtgtcacc aacgaagtgg
     2821 tcaggattgc cagcgcgtag cgaagggagc gtgcagttga agccatatta tctattcctt
     2881 tttgtaataa cttttttaca gagcataacc ttgtctaatg tctgagtcga ggatcatcaa
     2941 ttccggcttg ccatccaggc tcactcttag taacttttgc ccgcgaatga tgaggagatt
     3001 aagaatgctg aagaatctgg ctaaactgga tcaaacagaa atggataaag tgaatgtcga
     3061 tttggcggcg gccggggtgg cttttaaaga acgctacaat atgccggtga tcgctgaagc
     3121 ggttgaacgt gaacagccag aacatttgcg cagctggttt cgcgagcggc ttattgccca
     3181 ccgtttggct tcggtcaatc tgtcacgttt accttacgag cccaaactta aataaaactt
     3241 atacagagtt acactttctt acataacgcc tgctaaatta tgagtgtttt ctaaaccgcc
     3301 cccataattt gcagtcattt tgaaaaggaa gtcattatgt cgttctggaa agttgcagca
     3361 gcgcaatatg agcctcgtaa aacctcactc accgaacagg tagctcatca tctggagttt
     3421 gtgagggccg ccgccaggca acaatgtcag ctgttagttt ttccatcgct ttctttactg
     3481 gggtgtgatt attcgcgacg tgcccttcct gccccacccg atctttcact gttagacccg
     3541 ctttgttatg ccgcaacgac ctagcgaatg accatcattg ccggccttcc cgttgaatat
     3601 aacgatcgct ttattcgtgg aattgcggtg ttcgcaccgt ggcgaaaaac gcctgggaac
     3661 taccatcaga gtcatggtgc atgtctgggc cgtcgttcca gaaccatcac ggtggtcgat
     3721 gaacaaccgc aaggtatgga tatggacccg acctgttcac tgtttacgac agggcaatgt
     3781 ctgggagaac ccgacctgct ggcgtcggcc cgtcgcttac aatttttttc acatcagtac
     3841 tcgattgccg tgctgatggc aaatgcccgt ggtaacagtg cactatggga tgaatatggt
     3901 cgtctcatcg ttcgcgccga tcgtggttca ttattgttag tcggtcagcg ttcatcacag
     3961 ggttggcaag gcgatatcat tccattacgc taggcttttt cggcctggat catgccatgt
     4021 tgcgcattat cgatacagaa acctgcggtt tgcagggagg gatcgttgag attgcctctg
     4081 ttgatgtcat tgacggaaaa atcgtcaacc ccatgagcct cctggtgcgc cccgatcgtc
     4141 ctattagtcc acaagcgatg gcgattcatc gcatcaccga agccatggtc gccgataaac
     4201 cgtggattga agatgtgatc ccacactatt acggtagtga atggtatgtc gcgcacaacg
     4261 ccagctttga ccgccgcgta ctgcctgaga tgcccggtga gtggatttgc actatgaaac
     4321 tggcccgtcg tttgtggcca gggctcaagt acagcaatat ggcgttatat aaaacacgca
     4381 agctcaatgt acagacaccg ctgggcctgc atcatcaccg cgcgttgtat gactgttata
     4441 tcaccgccgc gttgcttatc gatattatga acacctccgg ctggacggca gaacagatgg
     4501 ccgatatcac cggacgtccg tcgttgatga caaccttcac ctttggcaaa taccgtggca
     4561 aagcggtttc cgacgttgcc gaacgctatc cgggctatct gcgctggtta tttaataacc
     4621 tggacagcat gagcccggag ctgcgtttaa cactgaaaca ttatctggaa aatacttaat
     4681 ccgcaggcgt agcgggtaat gttccctgcg ccagcgcgac cagaaaagca tattccatcg
     4741 ctacgccttc gtacgattta aagcggccag atttaccgcc atggcctgag tccatgtcgg
     4801 tacagagcaa taaaagatgg tcatcggttt tcagctcgcg caatttagcg acccattttg
     4861 ccggttccca atattgcacc tgagaatcgt gtaaaccggt cgttaccagt aaatgcggat
     4921 aagcctgtgc ggtgacgttg tcatacgggc tgtagctttt catgtactcg taatattgcg
     4981 gatcctgcgg atttccccac tcttcaaact caccagtggt aagaggaatt gattcatcaa
     5041 gcatcgttgt tacaacatca acaaacggta cctgggcgat aacgccgtgg aataattccg
     5101 ggcgttgatt aattgcaacg cccatcaaca tgccccccgc acttccgccc atcgcataac
     5161 agagcgaagg agagccatag cccagtttta acaatgcatc gcaggcatca agataatcat
     5221 taaacgtatt tttcttcttc agaaatttac cgtcttcgta ccattgttgc cccagctcac
     5281 caccgccgcg aacatggaca atggcgtaga caaagccacg atctaacaaa ctcaagcggc
     5341 taaaactgaa atcggcatca atacttgcgc cgtaagaacc atagccatac accagcaacg
     5401 ggttgtgtcc tttgcgaaaa tgtttgcgat gatagaccag cgaaaccgga acttcgacgc
     5461 catcacgggc gactatccac aggtgttcac tgcggtaatt cgccgcatca aaaccaggca
     5521 cttctgtttg ttttaataca cgacgctcac cggtatccat atccagttca aacaaagtgt
     5581 ctggtgtggt catggaagaa taaccataac gcaatcgcgc ggtttcaggt tctggattgt
     5641 aggcaatcca ggtcacatag gccggatcat caaaggcaat gccaatgact tcccgggtct
     5701 tgcggttaat ttggcgcaaa ctggttaacc cgcgctgacg ctcttcaacc accagccagt
     5761 cggtaaacag cgtaaaccct tccagcatga tgttttcgcg tggcggaatt aactcttccc
     5821 actgttgctc atcacgcata cgggtacggt ataagccaaa gtttttgccg tggcgattgg
     5881 aacgcagata aaaacgatgc tggtagtgat caaggctgta ttcgtgatct ttgcggcgcg
     5941 gcagaaaaac aaacggctcg gcatcggcca tttccgcgtc cagcaggcga acttcactgg
     6001 tggtggcgct ggccagatga atgacgacat agtgcttcga cgtcgtttta tgcaggctga
     6061 cataataggt atcgtctttt tcttcgtaga tcagtgtatc ttgcgatgct ggcgtaccga
     6121 tggcgtgacg ccagacctga taaggcagca gcgtcaccgg atgcttgcga acatagtaga
     6181 aagtccagga gtcatttgcc cagacaaagc tgggttcaac gttatccagc agttccgggt
     6241 accagttacc tgtttccaga ttacgaaaac gaatgccgta ctggcgtcgg gaaagaaaat
     6301 cttctgccag cgccataatg gtgttatcgg gcgtaatcgc cattccgccc atcgaataaa
     6361 actcactatg agctgcgcgc ttgttggcat cgagcaatgt ttcccactca tcccactctt
     6421 cgctgaatgc cgattgacgc tggtagatag catattcaca gcctggttca taaatatgcc
     6481 gatagcggta gccatttttt atgtagggcg cagaaacttc tctttgcgga atgcggtcga
     6541 tgatttcctt taagatgcga tcctgcaagg cttgttgtga ggccatcacc cgatgaccgt
     6601 aactattttc ttgttgcagg tagtccagga cttttggctg agaacgcgta tcgtcccgca
     6661 gccagtagta attatcgatg cgcgtatcgc catgaagcgt catggcgtgg ggaatgcggg
     6721 cggcttttgg tagcatgtta ttgttctttc tggttggaaa catcttataa gggtggcaaa
     6781 actcaccggg gatgcaagcg aaacagggtc gtcattgctt agatgatgac aggtaatggc
     6841 gcggatatcg aatgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgcggttc
     6901 tggatagccc gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt
     6961 gcgtttttgc tgttcgagat cgcgttcaat gccttcacga acatcctggg ggattttcag
     7021 cgcgtcaccc agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc
     7081 gcagctcagg aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaggcg
     7141 ttgtggatcc agcggttgtt cgattgcctg ctcaatgagt acacgcccct gctcttccac
     7201 gccggcttca cgcaattgct ggtcgattgc cgcacgttca ttggcatcaa tatgaccatc
     7261 acttttagcg gcaaagacca gcgcaaggat caaacgttcc gtacgctcat ccagcggcgt
     7321 actttgcgcg ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt
     7381 attccacagc accgtacccg ctaccgctcc gccgccaacc agtaacgcgt tggtgccata
     7441 tttcgtaaga agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa
     7501 tgcgcctgac actaacagtt tgcccaatcc ctgatccgca gacgaggagg tagaagaact
     7561 gctttgccca agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata
     7621 accattttta gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa
     7681 aagatgcgca aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg
     7741 tatttactgt atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt
     7801 ggcattgttt gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt
     7861 gtggtgcgtt ctgggacgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt
     7921 atcagaagcc attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgcagacc
     7981 tggtaatgaa tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct
     8041 ttaccctgaa tgtcctgcaa actgacccag gccacgggat cggcctgacc gacaattttt
     8101 tgatcatctg cccagcgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca
     8161 attccggcag catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta
     8221 ttggcagcga aaactgatgc gcaggcagaa accaacaacc gccctaaaaa cgcccctctt
     8281 tttttcatgt ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag
     8341 gagcgcaagt tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg
     8401 acctgtgggg acggtaattt gccggttttc agaatggtgc tcagcacctc tttttgttct
     8461 gccagccata gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc
     8521 agctaggtgt caagcagatc cgccgtatca agcattttgt cataagcatc ggcttccttt
     8581 ttgctggtaa acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc
     8641 gccatttgca gcctctcata ataactgaga ttttatacag tatatttctt ttcggttgag
     8701 aaatcaacat cagcaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt
     8761 aatcagggga tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc
     8821 gtgatgttat taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc
     8881 ggcgtagagg tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat
     8941 cgctcccatg tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa
     9001 aaaccacatt atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt
     9061 gaagaggaag gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc
     9121 gagggtatcc gtcgcctggc ggcagaagag ctgcagctgc ccacttccac ttatcgtttt
     9181 gccgatagca aaagcctttt ccgcgaggcg gttgccgcca ttggctatcc ctgcattgta
     9241 aaaccggtga tgagctcttc cggcaagggg cagacgttta ttcgctctgc agagcaactt
     9301 gctcaggcat gggaatacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt
     9361 gaaggcgtcg ttaagtttga cttcgaaatt accctgctaa ccgtcagcgc ggtggatggc
     9421 gtccatttct gtgcaccagt aggtcatcgc caggaagatg gcgactaccg tgaatcctgg
     9481 caaccacagc aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg
     9541 gtgctggcac tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag
     9601 gtgattttca gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct
     9661 caagatctct cagagtttgc cctgcatgta cgtgccttcc tcggacttcc tgttggcggg
     9721 atccgtcagt atggtcctgc agcttctgcc gttattctgc cacaactgac cagtcagaat
     9781 gtcacgtttg ataatgtgca gaatgccgta ggcgcagatt tgcagattcg tttatttggt
     9841 aagccggaaa ttgatggcag ccgtcatctg ggggtggtac tggctacttc agagagtgtt
     9901 gttgacgcca ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc
     9961 cggcaaaaaa acgctacaaa aatgcccgat cctagatcgg gcattttgac ttttacagct
    10021 tagcgccttc tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca
    10081 gcgcatctgc cggaaccagc caggaaccac cgatgcacag cacgcttttc agtgccaggt
    10141 agtcacggta gttagccgga gaaataccac ccgtcgggca gaaacggacc tgggagaacg
    10201 gacccgcgat cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga
    10261 actctttcaa accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa
    10321 tcagaggaat agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa
    10381 ttgcgaactg tgcacccgct tcagtgactt ctgccagctg ctgtggattc agcaccgtac
    10441 cggcacccac aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac
    10501 actcggtacg cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca
    10561 tcggcaccgc gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca
    10621 ggattgattc tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa
    10681 atttgtcgtc ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac
    10741 gcaaggcgct gaataattca cgtcctgttc ccacgcgtga cgcgctcagg tcaggaatgt
    10801 gcggttcgcg agcagccagt tccgcttcgt ctaccagcag tgtcagttcg cctgtctgtc
    10861 cattcacacg aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt
    10921 ctggtgttac gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg
    10981 taactaacgc aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat
    11041 gtaattctgg cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc
    11101 ggtccagcaa acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg
    11161 ctggcgcttc aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc
    11221 ccaggttacc gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag
    11281 cgatcacatt gctgtcgagt gatttttccg ccccttcccg ccagtccagt tcaccattat
    11341 tcagccatgg ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt
    11401 catgcagcag gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct
    11461 ggaagtggtt aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat
    11521 cagaaaggtc agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca
    11581 ccaggtgcat ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca
    11641 ccaccacttt ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca
    11701 tgcgtgtaac ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat
    11761 gaacaaaaga agagcctggc aactgcatcc ccataaattc caccaccatc tggttggtgt
    11821 tggcagtacc gtagaaagta catgttcccg gcgcatggta agacgcggct tctgactcca
    11881 gtaaggccat gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt
    11941 tatttggcaa accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa
    12001 acgacagggc tgccatcgtc agacccggga caatcttgtc gcacacaccg aggaacagag
    12061 caccatcaaa catgttatgg gacagcccca ccgccgcaga catcgctatc acttcgcggc
    12121 ttagcagcga caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa
    12181 caccgcccgc aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg
    12241 gatagtgttc ataaggctgg tgcgcggaga gcatgtcgtt ataggaggtg atgatggcga
    12301 tattgttacg caacatgctt ttcaaagagg ctttgttttc tggctggcag gcagcgaaac
    12361 cgtgtgccag gttaccgcat gccaactgcg aacgatgaac ggtcgacgtt ttcgcttgtt
    12421 ctatccgggc gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta
    12481 cgcgtaacaa ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt
    12541 cacaacgcgt ttcattcaga ggatttatga gtgaaacgcc tgtaaccgga gctcataggg
    12601 caaaaacgtt tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg
    12661 aaagtgtaaa aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa
    12721 aggataagcg ctgatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg
    12781 ccaccgaggc aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca
    12841 tcgcccacgc ctcagtaatg gagtctaccc atttccaggc ttcttccact tcgtcgcgac
    12901 gtacaaacag tgcctgaata ccacgcatgg tttccagcag caaacgttca taggcatccg
    12961 ccagatgcgt ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt
    13021 tatgtttgtg gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag
    13081 gttgcagacg gatagtcagt ttattctgcg gcagatcctg ccacgactct ttaaacagat
    13141 tcagttcagg tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac
    13201 cagtacgcag gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg
    13261 ccacgaaggt ttctgtattg ctgcttttgt tcgcgccctc ttcttccaga tatcccggca
    13321 cttttttgcc ctgggcgaag cccgcagtat attgcccgcg tacggttttt tcgcgtacgt
    13381 tggagcggtc gatgcggcgc agggacttca gtactttcac tttttcatcg cggatgctgt
    13441 ctgcgctcag gtcagatggc ggagacatcg caatcatgca gagaatttgt agcaggtggt
    13501 tctgaatcat gtcacgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc
    13561 ccacttcttc tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca
    13621 caaacaggga gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat
    13681 agtggtcgat acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt
    13741 cctgcgaggt cgccagcgac gtccccagcg gtttctccat gactacgcgt gccggtttag
    13801 cattcagttt tgcctcgcca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg
    13861 caaagtagtt aatagtgata cgattttttt gatccagcat cgcgccgaga cggttgaatg
    13921 cagcagtgtc attgacatcg agattacaaa aatccagacg tgcactcagg gtgtcccata
    13981 aaccttcatc aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca actttggtat
    14041 atgccgcttt atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct
    14101 gaccgacttt ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt
    14161 tcgcgccgaa aatgaccagg tcacaggcct gggctgtttg cgttaccgcc atgtcattct
    14221 ccttaagtta actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca
    14281 ctgtactgct tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg
    14341 gaaaagcgcg caaagcgcgg caaaactgat aaaaaaatca tcgttttcca ttcggtaaaa
    14401 atctgacact gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta
    14461 acacggcaca caggcgtaat atctgacaaa actgcatttc gatttctttc agtgcggaaa
    14521 tcgtcattac ccgtgagtct ctttacatca tgaatatgct ggaaaaaatc cagtctcagc
    14581 tggaacattt gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata
    14641 acgcgatcca ttcgagtatt gctgctctgg cgctggaagc caatgttagc gaaccgacgg
    14701 tgaatcgttt ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg
    14761 cacaaagtct ggcgaatggc actccctatg ttaatcgcaa cgtcaatgaa gatgacagcg
    14821 ttgaatcata cacggggaaa atatttgagt ccgcaatggc aacgctcgat catgtccgtc
    14881 attcactgga taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa
    14941 tcgccttttt cggattaggc tcttctgccg ccgttgccca cgatgcgatg aataagttct
    15001 ttcgttttaa tgttccggtg gtgtactccg atgatatcgt gctgcaacgc atgagttgta
    15061 tgaattgtag cgacggagac gtggtggtgc tgatttctca cacaggaaga acaaaaaatc
    15121 tggtcgagct ggcgcagctg gcacgcgaaa acgacgccat ggtgattgcc ctcacctctg
    15181 cgggtacccc gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata
    15241 ctgacattta tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg
    15301 cgacaggatt tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg
    15361 aagcgctgaa ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa
    15421 agcgactgtc actgtcctaa tcttatacga catccgaatg agattaattt atcgccatcg
    15481 cggcgttatt tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag
    15541 tattacatgt ccaaaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca
    15601 gatcgcgata ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac
    15661 ttttctcacg gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc
    15721 gcaaaactgg ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta
    15781 tccaccttta aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc
    15841 aacctgggta aaggtgaagg cgacaaagaa aaagtcggta tcgactacaa aggcctgcct
    15901 actgacgtcg tgcctggtga catcctgctg ctggacgatg gtcgcgtcca gttaaaagta
    15961 ctggaagttc agggcatgaa agtgttcacc gaagtcaccg tcggtggtcc cctctccaac
    16021 aataaaggta tcaacaaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac
    16081 aaagcagaca ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca
    16141 cgctgtggcg aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg
    16201 aaaattgttg ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac
    16261 atcatcctcg cctctgacgt ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc
    16321 gacccggaac tggtcggcat tcagaaagcg ttgatccgtc gtgcgcgtca gctaaaccga
    16381 gcggtaatca cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt
    16441 gcagaagtca tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct
    16501 gcagaaactg ccgctgggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc
    16561 ctgggtgcgg aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc
    16621 gacaatgtgg aagaagctat tgccatgtcg gcaatgtacg cagctaacca cctgaagggc
    16681 gttacggcga tcattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc
    16741 agctctggtc tgccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct
    16801 ctctatcgtg gcgttacgcc ggtgcccttt gatagcgcta atgacggcgt agcagctgcc
    16861 agcgaagcgg ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt
    16921 gtcacccaag gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tattttaacg
    16981 gtagagtaag tacgttgccg gatgcggcga aaacgccaca tccggcctat agttcaatga
    17041 tagttcaaca gatttcgaat attctgaagc aaacttgaac ttatcatcag gcgaaggcct
    17101 ctcctcgcga gaggcttttt tatttgatgg gataaagatc tttgcgctta tacggctgga
    17161 tttcgcccgg tttgcgagtt ttcagcaatt ttagtatcca ggtgtattgt tctggtcgcg
    17221 gaccaacaaa aatctcgact tcttcattca tccgccgctc aatcgtatga tcatccgcct
    17281 ctaacagatc atccatcggt gggcgcacct gaatcgtcag acgatgcgtc ttgccatcat
    17341 aaatcggaaa cagcggtaca acgcgcgcac ggcatacttt catcaaacga ccaatcgcgg
    17401 gcaacgtcgc tttataggtg gcaaagaaat ccacaaattc gctgtgctct gggccatgat
    17461 cctgatcggg taaataatat ccccagtacc cctgacgtac cgactggatg aatggtttaa
    17521 taccgtcatt tctcgcatgc agacgaccgc caaagcgacg acgcaccgtg ttccagacat
    17581 aatcaaaaac cgggttgccc tgattatgga acatcgctgc cattttctgc ccttgcgagg
    17641 ccatcagcat ggcaggaata tcgacggccc aaccgtgcgg caccagaaag ataactttct
    17701 cgttattacg ccgcatctct tcgatgatct ccagcccttg ccagccaacg cgcggctgaa
    17761 ttttctccgg cccgcgtatt gccaactcag ccatcattgc catcgcttgc ggcgcggtgg
    17821 caaacatctc atcaacaatc gcttcgcgtt cagcttcact acgttctgga aagcagagcg
    17881 acagattgat taacgcacga cggcgtgagc tttttcccag tcgtccggca aaacgtccca
    17941 gccgtgccag aatgggatca cggaactttg gcggcgttaa agcgataccc gccatcgctg
    18001 ctacacccag ccatgctccc cagtagcgcg ggtggcgaaa ggatttatca aactcaggaa
    18061 tgtattcgct attatttttt ttcgtttcca tgcttttcca gtttcggata aggcaaaaat
    18121 caatctggtg atagtgtagc ggcacaactt gccccgcacc aaataaaaaa agccggtact
    18181 gactgcgtac cggctgcgaa tggatgttaa ttaatcaaac cgtagctgcg gcacaatctc
    18241 tttggcctgc gccaggaatt cgcgacgatc ggagccggtc agcccttcgg tacgcggcag
    18301 ttttgccgtc agcgggttta cggcctgctg gtttatccat acttcatagt gcagatgcgg
    18361 cccagttgaa cgtccggtat taccggaaag cgcgatacgg tcgccacgtt tcaccttctg
    18421 tcccggtttc accagaatct tgcgcaagtg catataacgc gtggtgtagc tgcgaccatg
    18481 acgaatagcc acataataac ctgctgcgcc actgcgtttg gcaaccacca cttcaccgtc
    18541 acccactgaa agcactggcg taccttgtgg catggcgaaa tcaacacctc tgtgtggtgc
    18601 aacgcgacct gtcaccggat tagttcgacg cgggttaaag ttagacgaga tacggaactg
    18661 tttcgccgtc gggaatcgca agaatccttt cgccagacca gtaccgttac gatcgtagaa
    18721 tttgccatcc tcagcgcgga ttgcgtaata atctttacct tctgaacgca aacgtacgcc
    18781 cagcagctgg ctttgctcac gtttaccatc aagcatttct cgagacatta ataccgcaaa
    18841 ttcatcgcct tttttcagtt tgcggaaatc catttgccac tgcatggctt taatcactgc
    18901 gctcacttcg gcgctggtta aaccggcgtt tctggcgctg gcaacaaagc tccctccgac
    18961 ggtacctttc agcagattgt taacccactc tccttgctgc atttcgctgg tcattttaaa
    19021 accgttagcg gcagtacggt cataggttcg ggtttcacga cgagacactt cccaggtgag
    19081 gcgctgcagt tcgccgtccg cggttaatgt ccaggagagt tgttgaccga ttttcaggtt
    19141 acgcaattct ttgtcggcag cagcaagttg ggtgatatca cccatatcaa taccatactg
    19201 attgagaatg ctgcttagcg tatcgccagt ggaaacaaca tattcatgca cgcccgcttc
    19261 accggcgatt ttgtcatcca gttcgtcctg gggaatggct tcatcttctt gtgcagcttg
    19321 atcaatcggc tcactggctt caggtaagag cgaacgaatt tcgttctgtt ccagctcaat
    19381 ggttttgaca attggcgtgg catcacggtg ataaacatag ggccgccaga cagcgacggc
    19441 cagagtaaga acggtgagcg accccaacat aacgcggtgt ggtcgcggta aattattaaa
    19501 cgccagggcg acagagcggg ctatctgttg cacgtaatca cttcctcatt aatctccttt
    19561 caggcagctc gcatactggt tggctaattg actcaggaat tctgaatagc ttgttttacc
    19621 cagtttgata ttcgtcccca ggggatccaa cgttcccata cgaacggatg tccctcgtgc
    19681 gacgctctca acgaccgctg gcctgaactg tggctcagca aaaacgcagg ttgctttttg
    19741 ctcaaccaac tgtgttctta tttcatgtaa acgctgcgcg ccaggttgaa tctcagggtt
    19801 aacggtaaaa tgaccaagcg gtgtcagtcc gaactgtttt tcgaaatagc cgtaagcatc
    19861 gtgaaaaacg aaataacctt tccccttgag cggcgcgagc tcgttaccaa cctgcgtttc
    19921 ggttgaggcc aattgtgcct caaaatcctt caggttggcg tcaagtttgg ctcgactttg
    19981 cggcataagt tccactaatt ttccatggat tgcaaccgct gtagcccgcg ctatctctgg
    20041 ggaaagccaa agatgcatgt tgaaatcgcc gtgatggtga tcttcgtcac ttttttccgc
    20101 gtggtcgtga tcatcatcat cgccgtgaat acttttcatc agtagcggtt tcacatcttc
    20161 aagctgcgca atcgttacct gcttcgcttc tggtaatttg cttaccggtt tttgcataaa
    20221 cgcttccatc tccgggccaa cccaaacgac taagtccgcg ttctgtaagc gttttacatc
    20281 cgatggacgc agtgaataat catgttctga agcgccgtca ggcagtaaaa cctccgtttc
    20341 tgttacacca tcagcaatgg cagaagcgat gaacccaacg ggtttaagcg aagcgacaac
    20401 ggcggcatct gcggcctgtg ttgcacctcc ccagagagcg gcggataatg ctgcgaaaag
    20461 aagcgttttt ttatgtaaca taatgcgacc aatcatcgta atgaatatga gaagtgtgat
    20521 attataacat ttcatgacta ctgcaagact aaaattaaca tgacaagtct ggtttccctg
    20581 gaaaatgtct cggtttcttt tggccaacgc cgcgtcctct ctgatgtgtc gctggaactt
    20641 aaacctggaa aaattttgac tttacttggg ccaaatggcg caggtaagtc gacactggta
    20701 cgggtagtgc tcgggctggt aacacccgat gaaggggtta tcaagcgcaa cggaaaactg
    20761 cgcatcggct atgtaccgca gaagctgtat ctcgacacca cgttgccact gaccgtaaac
    20821 cgttttttac gcttacgccc tggtacacat aaagaagata ttttgcctgc actgaaacgt
    20881 gtccaggccg ggcacctgat taacgcaccg atgcaaaagc tctctggtgg cgaaacgcag
    20941 cgtgtactat tagcgcgagc attgttaaat tgcccgcaat tattagtgct ggatgaaccc
    21001 actcaaggcg tggatgtaaa tggccaagtg gcgttatatg accttattga ccagttgcgt
    21061 cgtgaactgg attgtggcgt tttaatggtt tctcacgatc tgcatctggt gatggcaaaa
    21121 accgatgaag tgctgtgcct gaatcaccac atttgttgtt ccggcacacc ggaagttgtt
    21181 tccctgcatc cggagtttat ttcaatgttt ggtcctcgtg gtgctgaaca actgggtatc
    21241 tatcgccatc atcataatca tcgtcacgat ttacagggac gaattgtttt gcgtcgggga
    21301 aatgatcgct catgattgaa ttattatttc ccggttggtt agccgggatc atgctcgcct
    21361 gtgccgcggg tccgctgggt tcgtttgtag tctggcgtcg tatgtcttat ttcggtgata
    21421 cgctggctca tgcctcatta cttggtgtcg cgtttggttt gttgctggac gtgaatccat
    21481 tctatgcggt gattgccgtt acgctgctgc tggcgggcgg tctggtatgg ctggagaagc
    21541 gtccacagct ggcgatcgac acgttattag ggattatggc gcacagtgcc ctgtcgctgg
    21601 gcctagtggt cgttagtctg atgtctaaca ttcgtgttga tttgatggct tacctgttcg
    21661 gtgatttact ggcagtgacg ccagaagatc tcatctctat tgcgattggc gtggtcatcg
    21721 tggtggctat tttgttctgg caatggcgca atttgctgtc gatgacgatt agcccggatc
    21781 tggcgtttgt tgatggtgtg aaattacagc aagtgaaatt gttgttgatg ctggtgacgg
    21841 cattgacgat tggtgtagcg atgaaatttg tcggtgcgtt gattattact tcgttgctga
    21901 ttattcctgc agctactgcg cgtcgctttg cccgtacgcc ggaacagatg gctggtgtcg
    21961 ctgttttggt ggggatggtg gcagtaactg gcggtttaac cttttccgcg ttttacgata
    22021 cgccggcggg tccgtcggtg gtgctatgtg cggcactgtt atttattatc agtatgatga
    22081 aaaagcaggc cagctaatct atcgctgaac acatttgtcg gatgcggcgc gagcgcctta
    22141 tccgacctac ggttcgctat ctctggctgg cctgataaga cgcgaacagt gtcgcatcag
    22201 acacactgcc agtgtcggat gcggcgcgag cgaccaatcc gacttacggc atttctggcg
    22261 gcgttatgcc aaagtgattc cacgcccgcg tcgtcgccat acgcccgcgc ggtgtacgct
    22321 gcaaaaagcc ttgctgaatc aaataaggtt ccagtacatc ttcaatggtt tcacgttctt
    22381 cgccaatggc tgccgccagg ttatccagac ctaccggccc accaaagaac ttatcgatta
    22441 ccgccagcaa caatttgcgg tccatataat cgaaaccttc agcatcgaca ttcaacatat
    22501 ccagcgcctg agcagcgata tctgccgaga tggtgccatc gtgcttcact tcggcgaaat
    22561 cacgcactcg acgcagcaga cggttggcaa tacgtggcgt accgcgagcg cgacgagcta
    22621 cttcctgcgc accgtcatca ctcatctcaa gccccataaa gcgtgcactg cgactgacga
    22681 tatattgcag atccggcacc tgataaaact ccagacgttg cacaatacca aaacggtcgc
    22741 gcaacggtga tgtcagcgaa cctgcgcgcg tggttgcgcc aatcagggta aacggcggca
    22801 aatcaatttt aatggagcgt gccgccggac cttcaccaat catgatatcc agttggtagt
    22861 cttccattgc cgggtacagc acttcttcaa caactggcga taaacggtgg atctcatcaa
    22921 taaacagcac gtcatgcggt tcaaggttag tgagcatcgc agccaaatcg cccgcctttt
    22981 ccagcaccgg accagaagtc gtgcgtaaat taacgcccat ttcattggcg acaatgttgg
    23041 caagcgtagt tttacccaac cccggaggac caaaaatcaa caaatgatcg agggcatcgc
    23101 cgcgcagttt cgctgctttg atgaaaatct ccatctgtga acgaacctgc ggctgaccaa
    23161 catactcttc cagtaattta gggcgaatgg cgcgatctgc tacatcttcc ggcaaagtgg
    23221 taccggcaga aatcagacgg tctgcttcaa tcatccttta cctcataacg cggcgcgtag
    23281 ggcttcgcga attaatgttt cactgctggt gtcagggcga gcgattttgc tcaccatgcg
    23341 gcttgcttct tgtggtttat agcctagcgc caccagcgcg gcaactgctt cttgttcagc
    23401 atcgtcggtc gccgggctag caggagacgt gagtaccagg tcggcggctg gcgtaaagag
    23461 atcgccatgc aaacctttaa atcggtcttt catttcaaca atcaagcgtt cggcggtttt
    23521 tttgccaata cccggcagtt tcaccagtgc ccccacttct tcacgctcaa cggcattaac
    23581 gaactgctgc gctgacattc cggagaggat cgccagggcc aacttcgggc cgacgccgtt
    23641 ggttttgatc aactctttga acaacgtgcg ttcttgttta ttgttaaaac cgtacagcag
    23701 ttgcgcgtct tcacgcacca caaagtgggt gaaaacgatc gcttcctgac ccgcttcagg
    23761 gagttcataa aaacaggtca tcggcatatg cacttcatag cctacgccgc ccacttcaat
    23821 taacaccagc gggggttgtt tttcaatgat gatgcctctg agtctgccta tcacatgacg
    23881 ctcctgcgta atgaatcaaa gataatgctg tatgataaaa aaatgctgga tagatatcca
    23941 gcgaaggatg aagaaaactt gcgaggtgtc ccgatgatct gaaaaatggc acagtataat
    24001 ttattcaaca ggttatattg gaagcaaata tttgaatatt acatattcag tgaagaaatg
    24061 cgtaataaaa atatacattg cgactcctga aaaataattt ttttatgcta ttacgtatat
    24121 ttgtatctat ttcaatggaa tgacaacgtg aatattaatt accctgctga atatgaaatt
    24181 ggagatatcg tctttacatg tataggtgct gccttatttg gtcaaatatc agctgcatca
    24241 aattgctgga gtaatcacgt cgggatcatt atcggtcata acggtgaaga cttcctggtt
    24301 gcagaaagcc gggttcccct ttcaaccatc accacgctat ctcgttttat taaacgttcg
    24361 tctaatcaac gctatgctat aaagcgatta gacgctgggc tgacagaaca acaaaaacaa
    24421 cgaattgttg aacaggttcc ctcccggcta cgcaaacttt accacacggg ttttaaatac
    24481 gaatcttcgc gccagttctg ttcaaaattt gtctttgata tttataaaga ggcgctatgt
    24541 attccggtgg gtgaaataga gacgtttgga caactgttaa atagcaatcc gaatgcaaaa
    24601 ctcactttct ggaaattctg gttcttaggt tctattccgt gggagcgtaa aaccgtcacg
    24661 ccagccagtt tgtggcatca tccgggtttg gtgttgattc acgcggaggg agttgaaacg
    24721 cctcagccgg aactgaccga ggcggtataa cttaacgcag tcgccctctc gtcaggttca
    24781 gccgcgattc gctcatctgc atcgcattct gactaacgtg gcagtgggtg atggcaatcg
    24841 ccagcgcatc ggcggcatcc gcctgtggat tagcgggcag tttcagcaag gtgcggacca
    24901 tatgctgcac ctggcttttt tcggcactac ccatacctac caccgtttgc tttacctgac
    24961 gtgccgcgta ttcaaatacc ggcaattcct gattcaccgc cgcaacaatc gccacgccgc
    25021 gcgcctggcc cagtttcagg gctgagtcag cgttcttcgc cataaagact tgttcaatgg
    25081 cgaaataatc aggctggaac tgggtgatga tttccgtcac gcccgcatag atgagcttca
    25141 gacgagacgg taaatcatcc actttggtgc gtatgcatcc gctacccagg taggacagtt
    25201 gcctacctac ctggcggatg acgccgtagc cggtcacgcg cgaacccggg tcaatgccga
    25261 gaataatagc catcacgcgt ctccgttttg ctgtttagca ggcatcatca gagagtcgct
    25321 gcaacctcat cagagatttc accgttatgg taaacttcct gcacgtcgtc gcaatcttcc
    25381 agcatatcga tcagacgcat cagtttcggc gcggtttctg catccatatc agctttggtg
    25441 gacgggatca tggaaacttc cgcgctgtct gctttcagac ctgccgcttc cagagcgtcg
    25501 cgcactttac ccatttcttc ccatgcagtg tagacatcaa tcgcgccgtc atcatatgtc
    25561 acaacgtctt cagcacctgc ttccagagct gcttccatga tggtgtcttc gtcgcctttc
    25621 tcgaaggaga tcacgccttt tttgctgaac agataagcta cggaaccgtc agtgccgagg
    25681 ttaccgccac atttgctgaa tgcgtgacgc acttcagcaa cggtacggtt gcggttgtca
    25741 gacagacatt caatcatgat tgccgtgccg ccaggaccgt aaccttcgta gatgatggtt
    25801 tccatgtttg catcatcatc accgcccaca ccacgtgcaa ttgcgcggtt cagtgtgtca
    25861 cgggtcatgt tgttagacag tgctttatcc accgccgcac gcagacgcgg gttagcgtcc
    25921 ggatcgccac cgcccagctt agccgcggtt accagctcac gaatgatttt agtgaagatt
    25981 ttaccgcgct tagcatcctg cgcagcttta cgatgtctgg tgttggccca tttactatga
    26041 cctgccataa aaatatctcc agatagccct gcctgttcag gcagcgttaa ttacaaactg
    26101 ttcaatcgcc tgccggttgc tccaggactt agtgagcgcc gccgcagcag gcgcatcaag
    26161 ccacttgtaa gccagatgtt cagtgaaaac gatctgccgc tcgtgcggaa gcgcaagaca
    26221 gaaccatgat tccgtattac gcgtcacgcc cggcgcatag cgatgacgta aatgtgaaaa
    26281 aatttcaaac tctaccgtgc gctgacagtc aattaaggtc agttgttcag cgacaacatc
    26341 aatggtaacc tcttccttta cttcgcgcat ggcagcttgc ggcgcggttt caccctcttc
    26401 cacgctgccg gttaccgact gccagaaatc gggatcgtca cgccgctgca acatcagcac
    26461 ccgtttcgta tcttgtgcat agatgaccac taagatcgaa acgggacgct tatacacttt
    26521 atccttcacg ctgcctcttc gttgactgcc ttcgctcatc ccattcacat agttatctat
    26581 gctcatggga gttcactcag ttgccgcctt gatgcaacgc gaatgatttc gtgtatttga
    26641 gtcatatcag ttattctcag ccttcttcac aacctgaatg ctcagctcag ccagtgcagt
    26701 cgggttagca aagctcggtg cttcagtcat cagacacgct gccgccgtgg ttttcgggaa
    26761 ggcgataacg tcacggatat tgtcggtgcc agtcagcagc atggtcagac ggtcaagacc
    26821 gaatgccaga cctgcgtgcg gcggagtacc gtatttcagg gcgtcgagca ggaagccgaa
    26881 tttctcgcgc tgttcctctt cgttgatacc cagaatacca aacaccgtct gctgcatatc
    26941 accattatgg atacgtactg aaccaccgcc cacttcgtaa ccattgatga ccatatcgta
    27001 agcgttcgcc accgcatttt ccggtgcagc tttcagttct gcagcagtca tatctttcgg
    27061 tgaggtgaac ggatggtgca ttgccgtcag gccgccttca ccgtcgtctt caaacatcgg
    27121 gaagtcgata acccacagcg gtgcccattt gctttcgtcg gtcagaccaa ggtctttacc
    27181 cactttcagg agcagtgcgc ccatcccgtc ggcaacaatt ttcttgttgt cggcaccgaa
    27241 gaaaatcata tcgccatctt gcgcggcagt acgatccagg atggcttcga tgatttctgc
    27301 attaaggaac ttcgctaccg ggctgttgat accttccaga cctttcgcgc gttcgttaac
    27361 tttgatgtaa gccagacctt tcgcgccgta gattttaacg aagttaccgt attcgtcgat
    27421 ctgcttacgg gtcagcgatg cgccacccgg aacacgtaga gcggcaacac ggcctttcgg
    27481 atcgttcgct ggccccgcaa atactgcaaa ctcaacagat ttcagcagat cggcaacgtc
    27541 ggtcagttcc atcgggttac gcagatccgg tttatcagaa ccataacggc gttctgcttc
    27601 cgcaaaggtc attaccggga aatcgcccag atccacgccc ttcacttcca gccacaaatg
    27661 acgcaccagc gcttccatca cttcacgcac ttgcggcgcg gtcatgaaag aagtttccac
    27721 atcgatctga gtaaattcag gctgacgatc agcgcgcagg tcttcgtcac ggaagcattt
    27781 aacgatctga tagtaacggt caaaaccgga catcatcagc agctgtttga acaactgtgg
    27841 ggattgcggc agtgcgtaga atttaccttt gtgcacacga gaaggcacca ggtagtcacg
    27901 cgcgccttcc ggcgtggctt tggtcagcat cggagtttcg atgtcgagga agccgtggtc
    27961 atccataaaa cggcgcacca ggctggtgat tttagcgcgg gttttcaggc gttgcgccat
    28021 ttccgggcga cgcaggtcga ggtagcggta tttcagacgc gcttcttcgg tgttgacgtg
    28081 gttagagtca agcggcagaa catctgcgcg gttgatgata gtcagcgagg acgccagcac
    28141 ttcaatttcg ccggtcgcca tatcgcggtt aatatttttt tcgtcacgcg cacgtacggt
    28201 gcccgtaacc tgaatgcaga actcattacg cagttcagag gccagcttta acgcgtccgc
    28261 acgatccgga tcgaaaaata cctgcacgat accttcgcgg tcgcgcatat cgatgaagat
    28321 caggctacca agatcacgac gacggttgac ccaaccacac agagtcacct gctgccccac
    28381 gtgggacaaa cggagctgtc cacaatattc tgtacgcatg agatatccct taacttagct
    28441 gccggcggat gccccctgct gcgcaggtga ccaagtcgca gcgttagctg tatgtcacaa
    28501 ctgaatgaaa aaaggcggct attatactgg aaattctgcc gcaccgtaag agcctggccc
    28561 gcgctggaac gcctcgttac cactttatat cgggcctgaa atcagactct acgccagttt
    28621 gctataaagg tgttgcccga actcataaaa attaacaaaa tttgtcgttc cgccatcggc
    28681 taatcgcatt aaggtgtgag gcacgatttt gttttttcag gagtcagcat gcttgaactt
    28741 aatgctaaaa ccactgcgtt ggtggtgatc gatttacaag aaggcatctt accttttgcc
    28801 ggtggtccac atactgccga tgaggtagtt aatcgcgccg ggaagctggc ggcgaaattt
    28861 cgcgccagcg gtcagcccgt gtttctggtg cgagttggct ggtctgccga ttacgccgaa
    28921 gcattaaaac agccggttga t
//
LOCUS       DAJTNP010000046        28915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTNP010000046 DAJTNP010000000
VERSION     DAJTNP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO160_002187"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO160_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5524298.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO160_002188"
     CDS             complement(2138..2584)
                     /locus_tag="OO160_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5524299.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO160_002189"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO160_002189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5524300.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO160_002190"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO160_002190"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5524301.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO160_002191"
     CDS             complement(5347..6009)
                     /locus_tag="OO160_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5524302.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO160_002192"
     CDS             6182..6595
                     /locus_tag="OO160_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5524303.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO160_002193"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO160_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5524304.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO160_002194"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO160_002194"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5524305.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO160_002195"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO160_002195"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5524306.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO160_002196"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO160_002196"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5524307.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO160_002197"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO160_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5524308.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO160_002198"
     tRNA            complement(9861..9948)
                     /locus_tag="OO160_002198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO160_002199"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO160_002199"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5524309.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO160_002200"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO160_002200"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5524310.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO160_002201"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO160_002201"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5524311.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO160_002202"
     CDS             14005..14592
                     /locus_tag="OO160_002202"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5524312.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO160_002203"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO160_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5524313.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO160_002204"
     CDS             14984..15841
                     /locus_tag="OO160_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5524314.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO160_002205"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO160_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5524315.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO160_002206"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO160_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5524316.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO160_002207"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO160_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5524317.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO160_002208"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO160_002208"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5524318.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO160_002209"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO160_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO160_002210"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO160_002210"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5524319.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO160_002211"
     CDS             complement(22884..24023)
                     /locus_tag="OO160_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5524320.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMDEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO160_002212"
     CDS             complement(24069..26165)
                     /locus_tag="OO160_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5524321.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO160_002213"
     CDS             complement(26165..26911)
                     /locus_tag="OO160_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5524322.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO160_002214"
     CDS             complement(26908..27552)
                     /locus_tag="OO160_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5524323.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO160_002215"
     CDS             complement(27659..27964)
                     /locus_tag="OO160_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524324.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO160_002216"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO160_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5524325.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc atccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agttttaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DAJTNP010000047        28669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTNP010000047 DAJTNP010000000
VERSION     DAJTNP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..648
                     /locus_tag="OO160_002217"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO160_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="OO160_002218"
     CDS             645..1031
                     /locus_tag="OO160_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524326.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="OO160_002219"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="OO160_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5524327.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="OO160_002220"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="OO160_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="OO160_002221"
     CDS             3759..4166
                     /locus_tag="OO160_002221"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5524328.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="OO160_002222"
     CDS             4163..6451
                     /locus_tag="OO160_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5524329.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6448..7437
                     /locus_tag="OO160_002223"
     CDS             6448..7437
                     /locus_tag="OO160_002223"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524330.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="OO160_002224"
     CDS             7537..7740
                     /locus_tag="OO160_002224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524331.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="OO160_002225"
     CDS             7758..8078
                     /locus_tag="OO160_002225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524332.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="OO160_002226"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="OO160_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO160_002227"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO160_002227"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5524333.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="OO160_002228"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="OO160_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5524334.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO160_002229"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO160_002229"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5524335.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="OO160_002230"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="OO160_002230"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5524336.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="OO160_002231"
     CDS             13394..14161
                     /locus_tag="OO160_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5524337.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="OO160_002232"
     CDS             complement(14132..14872)
                     /locus_tag="OO160_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5524338.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15256..15777
                     /locus_tag="OO160_002233"
                     /pseudo
     CDS             15256..15777
                     /locus_tag="OO160_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="OO160_002234"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="OO160_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5524339.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18379)
                     /locus_tag="OO160_002235"
                     /pseudo
     CDS             complement(16667..18379)
                     /locus_tag="OO160_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="OO160_002236"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="OO160_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5524340.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="OO160_002237"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="OO160_002237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5524341.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="OO160_002238"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="OO160_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5524342.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="OO160_002239"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="OO160_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5524343.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="OO160_002240"
     CDS             21018..21470
                     /locus_tag="OO160_002240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5524344.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="OO160_002241"
     CDS             21716..22759
                     /locus_tag="OO160_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5524345.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22821..23435
                     /locus_tag="OO160_002242"
                     /pseudo
     CDS             22821..23435
                     /locus_tag="OO160_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO160_002243"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO160_002243"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5524346.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..25669
                     /gene="gpt"
                     /locus_tag="OO160_002244"
     CDS             25211..25669
                     /gene="gpt"
                     /locus_tag="OO160_002244"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5524347.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25761..27005
                     /gene="frsA"
                     /locus_tag="OO160_002245"
     CDS             25761..27005
                     /gene="frsA"
                     /locus_tag="OO160_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5524348.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27063..27464
                     /gene="crl"
                     /locus_tag="OO160_002246"
     CDS             27063..27464
                     /gene="crl"
                     /locus_tag="OO160_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5524349.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="OO160_002247"
     CDS             complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="OO160_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5524350.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7081 a         7124 c         7606 g         6858 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaccatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgccag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ttcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttcttgctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggcgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    25561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
    25621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    25681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
    25741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    25801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    25861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    25921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
    25981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
    26041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
    26101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
    26161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
    26221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
    26281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
    26341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
    26401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
    26461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
    26521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
    26581 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
    26641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
    26701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
    26761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
    26821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
    26881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
    26941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
    27001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
    27061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
    27121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
    27181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
    27241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
    27301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
    27361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
    27421 cggcgaatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
    27481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
    27541 cattattaat attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
    27601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
    27661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
    27721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
    27781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
    27841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
    27901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
    27961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
    28021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
    28081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
    28141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
    28201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
    28261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
    28321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
    28381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
    28441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
    28501 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
    28561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
    28621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatatt
//
LOCUS       DAJTNP010000048        28493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTNP010000048 DAJTNP010000000
VERSION     DAJTNP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(92..229)
                     /gene="yjdI"
                     /locus_tag="OO160_002248"
                     /pseudo
     CDS             complement(92..229)
                     /gene="yjdI"
                     /locus_tag="OO160_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
     gene            410..2041
                     /gene="dcuS"
                     /locus_tag="OO160_002249"
     CDS             410..2041
                     /gene="dcuS"
                     /locus_tag="OO160_002249"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5524351.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2038..2757
                     /gene="dcuR"
                     /locus_tag="OO160_002250"
     CDS             2038..2757
                     /gene="dcuR"
                     /locus_tag="OO160_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5524352.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3328..4668
                     /gene="dcuB"
                     /locus_tag="OO160_002251"
     CDS             3328..4668
                     /gene="dcuB"
                     /locus_tag="OO160_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5524353.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4746..6392
                     /gene="fumB"
                     /locus_tag="OO160_002252"
     CDS             4746..6392
                     /gene="fumB"
                     /locus_tag="OO160_002252"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5524354.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6515..7144
                     /locus_tag="OO160_002253"
     CDS             6515..7144
                     /locus_tag="OO160_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5524355.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7283..8704)
                     /gene="melB"
                     /locus_tag="OO160_002254"
     CDS             complement(7283..8704)
                     /gene="melB"
                     /locus_tag="OO160_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5524356.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8807..10162)
                     /gene="melA"
                     /locus_tag="OO160_002255"
     CDS             complement(8807..10162)
                     /gene="melA"
                     /locus_tag="OO160_002255"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5524357.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10445..11353
                     /gene="melR"
                     /locus_tag="OO160_002256"
     CDS             10445..11353
                     /gene="melR"
                     /locus_tag="OO160_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5524358.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11552..13819
                     /gene="adiA"
                     /locus_tag="OO160_002257"
     CDS             11552..13819
                     /gene="adiA"
                     /locus_tag="OO160_002257"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5524359.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14144..14905
                     /gene="adiY"
                     /locus_tag="OO160_002258"
     CDS             14144..14905
                     /gene="adiY"
                     /locus_tag="OO160_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5524360.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            15042..16379
                     /gene="adiC"
                     /locus_tag="OO160_002259"
     CDS             15042..16379
                     /gene="adiC"
                     /locus_tag="OO160_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5524361.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16483..18126
                     /gene="eptA"
                     /locus_tag="OO160_002260"
     CDS             16483..18126
                     /gene="eptA"
                     /locus_tag="OO160_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5524362.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18123..18791
                     /gene="basR"
                     /locus_tag="OO160_002261"
     CDS             18123..18791
                     /gene="basR"
                     /locus_tag="OO160_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5524363.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18792..19892
                     /gene="pmrB"
                     /locus_tag="OO160_002262"
     CDS             18792..19892
                     /gene="pmrB"
                     /locus_tag="OO160_002262"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5524364.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19868..19957)
                     /gene="pmrR"
                     /locus_tag="OO160_002263"
     CDS             complement(19868..19957)
                     /gene="pmrR"
                     /locus_tag="OO160_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5524365.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(20069..21571)
                     /gene="proP"
                     /locus_tag="OO160_002264"
     CDS             complement(20069..21571)
                     /gene="proP"
                     /locus_tag="OO160_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5524366.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21835..22713)
                     /locus_tag="OO160_002265"
     CDS             complement(21835..22713)
                     /locus_tag="OO160_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5524367.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22710..24938)
                     /gene="crfC"
                     /locus_tag="OO160_002266"
     CDS             complement(22710..24938)
                     /gene="crfC"
                     /locus_tag="OO160_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5524368.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25341..25676
                     /locus_tag="OO160_002267"
     CDS             25341..25676
                     /locus_tag="OO160_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5524369.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25834..26277
                     /gene="yjdN"
                     /locus_tag="OO160_002268"
     CDS             25834..26277
                     /gene="yjdN"
                     /locus_tag="OO160_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5524370.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26410..27198
                     /gene="phnC"
                     /locus_tag="OO160_002269"
     CDS             26410..27198
                     /gene="phnC"
                     /locus_tag="OO160_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5524371.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27223..28239
                     /gene="phnD"
                     /locus_tag="OO160_002270"
     CDS             27223..28239
                     /gene="phnD"
                     /locus_tag="OO160_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5524372.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28345..>28493
                     /locus_tag="OO160_002271"
     CDS             28345..>28493
                     /locus_tag="OO160_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5524373.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7325 a         7435 c         6953 g         6780 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttaaataagt gatactggtt
       61 gtctggagat tcagggggcc agtctacacg actttcgttt gagattaaat aacttgccgt
      121 tgccacgtac gcaattgcca gaatgctgac atatcgcagt gttgaaatag acatcgatct
      181 tttcgccggt ataacagcga taacccccgt ccagtagcgc ctgatccatg accttccctc
      241 tctcatcagt atgattatcg ctataagcat agccccggaa tcttcataca gcactggcag
      301 tttccgttgc cagagtctct tcccctatat agccaatcat ttttactccc tttgaattac
      361 ccgcctcatc agagataatg cttaagaaat ttgtcacaca aggaagctga tgagacattc
      421 attgccctac cacatattac gcaaacgtcc gatgaaattg agtaccactg tgatcttaat
      481 ggtcagcgcg gtactgttct cggtgctatt ggtggtgcat ctgatttact tctcgcaaat
      541 cagtgatatg acgcgagacg ggctggccaa caaggcactg gcagtggcgc gtaccctcgc
      601 cgactcgccg gaaatccgtc agggcttgca gaaaaaaccg caggagagtg gcatccaggc
      661 catcgcggaa gccgtgcgca aacgcaacga tctgctgttt attgtcgtta ccgatatgca
      721 aagtcttcgc tactcacatc ctgaagccca gcgtattggt cagccattta aaggtgatga
      781 catccttaat gcgctgaatg gcgaagaaaa tgtcgctatc aatcgcggtt ttctggcgca
      841 ggctttacgc gtatttaccc ccatctacga tgaaaatcat aaacaaattg gcgtggtggc
      901 gatcggcctt gagttaagcc gcgtgaccca acagatcaat gacagtcgct ggagcattat
      961 ctggtcggta ttatttggca tgctggtcgg actgattggc acctgcattc tggttaaggt
     1021 actgaaaaaa atccttttcg gcctggaacc ctacgaaatc tccacgctgt ttgagcaacg
     1081 ccaggccatg ttgcagtcta tcaaagaagg cgtcgttgcc gtggacgatc gcggcgaggt
     1141 cacgctgatc aacgatgccg cacaagaatt gctgaattac cgtaagtcgc aggacgatga
     1201 gaaactgtcg acgctcagcc actcatggtc acaggtggta gatgtctcgg aagtgttacg
     1261 cgacggtacg ccgcgccgcg acgaagagat tacgattaaa gaccggctat tactgatcaa
     1321 caccgttccg gtgcgcagta atggcgttat catcggtgcc atttcaacct tcagggacaa
     1381 aactgaagtt cgtaaactga tgcagcgact cgacggtctg gtcaactatg ctgacgcact
     1441 tcgtgaacga tcccacgaat ttatgaataa attgcatgtg attctcggat tattgcatct
     1501 gaagagttat aagcagttgg aagattacat tctcaaaaca gccaataact atcaggaaga
     1561 gattggctct ctgctgggta agatcaaatc tccggttatc gctggttttt taatcagcaa
     1621 gattaaccgc gcgaccgatt taggccatac gctgatttta aacagtgaaa gccagctgcc
     1681 agacagcggc agtgaggacc aggtcgcgac gctgattacc acattgggaa atctgataga
     1741 aaacgcgctg gaagcattag ggccggaacc cggtggcgaa attagcgtaa cattgcacta
     1801 ccgtcacggc tggctgcact gcgaagttaa cgatgatgga ccggggatcg cacccgacaa
     1861 aatcgatcac atttttgaca aaggtgtctc gacaaaagga agcgagcgag gcgtcggttt
     1921 agcacttgtc aaacaacagg tagaaaatct cggcggcagc atcgccgtgg aatcggaacc
     1981 ggggattttc acacaatttt ttgtccagat accctgggac ggggagaggt cgaacagatg
     2041 atcaatgtat taattatcga tgacgacgca atggtcgcgg agctgaatcg ccgatacgta
     2101 gcacaaatcc caggctttca atgctgtgga acagcctcga cgctggagaa agccaaagag
     2161 attatcttca atagcgatgc gcctatcgac ctgatattgc tcgatatcta tatgcaaaaa
     2221 gagaacgggc tcgatttact gcctgtcctg cataacgcgc gttgcaaaag tgatgtgatt
     2281 gtcatctcct ccgcagccga tgcggcaacc attaaagatt cgctgcatta cggtgtcgtg
     2341 gattacctga tcaaaccctt ccaggcttcc cgctttgaag aggcgctcac cggctggcgg
     2401 caaaagaaaa tggcgctgga aaaacatcag tattatgacc aggccgagct tgatcagcta
     2461 atccacggca gcagctccaa cgaacaggat cctcgtcgct tgccaaaagg cttaacgccg
     2521 cagacgttac gcacgctgtg ccagtggatt gacgcgcatc aggactatga gttttcaacc
     2581 gacgagctgg ctaacgaggt taacatctcg cgtgtttcct gccgaaaata cctcatctgg
     2641 ctggtcaact gccacatctt gttcaccagt atccattatg gcgtcacggg gcgtccggtt
     2701 taccgttacc gtattcaggc agaacactac tcactgctga aacaatattg ccaataacgg
     2761 atcgctggtt atctgtaagt aataattatt accgtaatgc aaaatacccc tggcacaagt
     2821 tacatcgtta atcagtaaaa cttgtgccag atcaaatata attatccctc catcatcgtt
     2881 aaaaattaat atctcttcag atgaacggtg tttttaattt caaaacgata acaaaagtta
     2941 attaactatt atgtcacccg cattatgtgt atttttaccc acaaatgggt agatcagatt
     3001 aatctataaa cctaatgata tctgccctga gaacaaaaaa tagaccgata aatatcaata
     3061 agataacagc aaacaaaaca ttaacatctg cgcagtacaa actataaacc catcgccaga
     3121 gagtttttct ctctgaaaaa gccgcttatc acagtgcata aatttgccgc tgctttaatc
     3181 agccaatatt cactgtgagg tatttgctaa agccggtaac gaccaaacgg atatttagtc
     3241 aggctctgaa aacagttcat acaaaacaga acgtgactgt gatctattca gcaaaaattt
     3301 aaataggatt atcgcgaggg ttcacacatg ttatttacta tccaacttat cataatacta
     3361 atatgtctgt tttatggtgc cagaaagggt ggtatcgcgc tgggtttatt aggcggtatc
     3421 ggtctggtca ttctggtctt cgtcttccac cttcagccag gtaaaccacc agttgatgtc
     3481 atgctggtta tcattgcggt ggtggcggca tcggcgacct tgcaagcttc gggcggtctt
     3541 gatgtcatgc tgcaaattgc cgagaagctg ctgcgccgca acccgaaata tgtctcaatt
     3601 gtcgcgccgt ttgtgacctg tacactgacc attctttgcg gtacgggtca tgtggtttac
     3661 accattctgc cgatcatcta cgacgtcgcc attaagaaca acatccgtcc ggaacgtccg
     3721 atggcggcaa gttctatcgg tgcacagatg gggattatcg ccagtccggt gtcggttgcg
     3781 gtcgtgtctc tggttgcgat gctgggtaat gtcacctttg atggtcgcca tcttgagttc
     3841 ctcgatctgc tggcaatcac cattccatcg acgttaatcg gtatcctggc gatcggtatc
     3901 ttcagctggt tccgcggtaa agatctggat aaagacgaag agttccagaa attcatctcc
     3961 gtaccggaaa accgtgagta tgtttacggt gataccgcga cgctgctgga taaaaaactg
     4021 ccgaaaagca actggctggc aatgtggatt ttcctcgggg caatcgctgt agtcgccctt
     4081 cttggtgctg attcggacct gcgtccatcc ttcggcggca aaccgctgtc gatggtactg
     4141 gttattcaga tgtttatgct gctgaccggg gcgctgatta ttatcctgac caaaaccaat
     4201 cccgcgtcta tctcaaaaaa cgaagtcttc cgttccggta tgatcgccat cgtggcggtg
     4261 tacggtatcg catggatggc agaaaccatg ttcggtgcgc atatgtctga aattcagggc
     4321 gtactgggtg aaatggtgaa agagtatccg tgggcgtatg ccattgttct gctgctggtt
     4381 tccaagtttg taaactctca ggctgcggcg ctggcggcga ttgttccggt cgcgctggcg
     4441 atcggcgttg atccggcata catcgtggct tcagcaccgg cttgctacgg ttattacatc
     4501 ctgccgactt atccgagcga tctggcagcg attcagtttg accgttccgg caccacccac
     4561 atcggtcgct tcgtcatcaa ccacagtttt attctgccgg ggttgattgg tgtgagcgta
     4621 tcgtgcgtct tcggctggat cttcgccgcg atgtacgggt tcttataaat gcactttgcg
     4681 tgccgcccgt gactacgcgg cacgccattt tcgaataaca aatacagagt tacaggctgg
     4741 aagctatgtc aaacaaaccc tttatctacc aggcaccttt cccgatgggg aaagacaata
     4801 ccgaatacta tctactcact tccgattacg ttagcgttgc cgacttcgac ggcgaaacca
     4861 tcctgaaagt ggaaccagaa gccctgaccc tgctggcgca gcaagccttt cacgacgctt
     4921 ctttcatgct acgtccggca caccagaaac aggttgcggc tattcttcac gatccagaag
     4981 ccagcgaaaa cgacaagtac gtggcactgc aattcttaag aaactccgaa atcgccgcca
     5041 aaggcgtgct gccgacctgc caggataccg gcaccgcgat catcgtcggt aaaaaaggcc
     5101 agcgcgtgtg gaccggtggc ggtgatgaag aagcgctgtc gaaaggcgtc tataacacct
     5161 atatcgaaga taacctgcgc tactcgcaaa acgcggcgct ggacatgtac aaagaggtca
     5221 acaccggcac taacctgcct gcgcaaatcg acctgtacgc ggtagatggc gatgagtaca
     5281 aattcctttg cgttgcgaaa ggcggcggct ctgccaacaa aacgtatctc taccaggaaa
     5341 ccaaagccct gctgactccc ggcaaactga aaaacttcct cgtcgagaaa atgcgtaccc
     5401 tcggtactgc agcctgcccg ccgtaccata tcgcgtttgt gattggcggt acgtctgcgg
     5461 aaaacaacct gaaaaccgtc aaattagcaa gcgctcacta ttacgatgaa ctgccgacgg
     5521 aagggaacga acatggtcag gcgttccgcg atgtccagct ggaacaggaa ctgctggaag
     5581 aggcccagaa actcggtctt ggcgcgcagt ttggcggtaa atacttcgcg cacgacattc
     5641 gcgttatccg tctgccacgt cacggcgcat cctgcccggt aggcatgggc gtctcctgct
     5701 ccgctgaccg taacattaaa gcgaaaatca accgcgaagg tatctggatc gaaaaactgg
     5761 aacacaatct aggtcagtac attccacaag aactgcgtca ggccggtgaa ggcgaagcgg
     5821 tgaaggtcga ccttaaccgc ccgatgaaag agatcctcgc ccagctttcg caatatccgg
     5881 tttccactcg tttgtcgctc actggcacca ttattgtggg tcgagatatt gcacacgcca
     5941 agctgaaaga gctgattgac gccggtaaag aacttccgca gtacatcaaa gatcacccga
     6001 tctactacgc gggtccggcg aaaacccctg ccggttatcc atcaggttca cttggcccaa
     6061 ccaccgcagg tcgtatggac tcctatgtgg atctgctgca atcccacggc ggcagcatga
     6121 tcatgctggc gaaaggcaac cgcagccagc aggttaccga cgcgtgtcat aaacacggcg
     6181 gcttctacct cggtagcatc ggcggtccgg cggcggtact ggcgcagcag agcatcaagc
     6241 atctggaatg cgtcgcttat ccggagctgg gtatggaagc tatctggaaa atcgaagtag
     6301 aagatttccc ggcgtttatc ctggtcgatg acaaaggtaa cgacttcttc cagcaaatcg
     6361 tcaacaaaca gtgcgcgaac tgcactaagt aacctcttcg gcccagcgcc tggcagcatg
     6421 ctgccaggtg atccccctgg gccacctctt ttgcgattgt aatttcatgc ttgctggtga
     6481 atagtcagta ttttccctga cttgcgaact caccatgacc cgcacactca agccgttaat
     6541 tcttaacact ggcgcactgg cgctaacgtt aatcctgatt tataccggca tttcggccca
     6601 tgacaaactc acctggctgc tggaagtgac accggtgatt atcgtcgtgc cgctactact
     6661 tgccaccgcc agacgttacc cgttaacgct gctgctctat acgctcatct tctttcacgc
     6721 catcatcctg atggtaggcg ggcagtacac ctacgcgaaa gtccccgttg gttttgaggt
     6781 gcaggaatgg ctggggttga gtcgtaatcc gtatgacaag ctggggcact ttttccaggg
     6841 gctggtgcct gcactggtgg cacgagaaat tctcgttcgc gggatgtacg tgcgcggacg
     6901 taaaatggtg gcgtttctgg tctgttgcgt ggcgctggcg ataagcgcga tgtatgaatt
     6961 aatcgagtgg tgggcggcac tggcgatggg ccagggagcg gatgattttc tcggtaccca
     7021 gggcgaccag tgggacacgc aatccgatat gttctgcgcg ctgcttggcg cattaacgac
     7081 agtgatattg ctcgctcgct ttcattgccg ccagctacgg cgctatggct tgataactgg
     7141 ataacaacgt gcactttgtt gatgccggat gcggcgtgaa ttccttatcc ggtctacaaa
     7201 atttgcaaat ccaaagcatt acattaagac gtggcctgat aaacatatcg catcaggcca
     7261 ttttcgttag ctattgtcca cgtcaggctt tcacatcact cactgcaccg accggaatat
     7321 cagcatgaac aggctcgggc ggtactttgc gatatttatc cagcagatga atctggatcc
     7381 tgcgcagcgt gtcgccattg aggcgataga aacggaagta gagaatcagc gttaccatga
     7441 aaaacagagt cggtagagca atcataataa actgcatacc taatagggct tgcgtagact
     7501 gttcaacatt cggtacatag ccaatcatcc ctaacacaac cgcaatgaaa aaagccgcaa
     7561 aggctgaacc gcccttcacc accatagtct gcacggagta agcgatgctt tcacagcgta
     7621 cgtgcagttt atattcaccg taatcaacgg tatctgccac catgatgacc tgtaataccc
     7681 agaaaagcgc cgttcccaca ttcagcaaaa tacccgcaat cacaatgagg acgacgttgt
     7741 gatagctcat taatgccatt aacaggagaa caccacagct taacaccgga agaatagatg
     7801 ctccggccca taaaatgcgt cgggataatg atttaactaa gcgtgggaag aatactaacg
     7861 tcaccaggtt agcagctccc gcatacgaca gataataggg gaacaaatcc gcatcaccga
     7921 taacatatga gaaataatag atagcaaatc cggtaataat gttgctggct acattataag
     7981 caagagccat acccaagagg catgaaagct gatcgttttt ataaataagc gcaacgatgg
     8041 ctttaagtgt cagatggctt ccttcagcag acgattgatt gtctgacgaa aagacttcat
     8101 gcacattgcg cagagtgatg atggttgaaa caataaaaaa ggcgatcaat accagagtga
     8161 acatctgaaa gccaaatccc cgatcgccac cgccgacata attaacaaat ggtagcgtca
     8221 cacctgccgt aacaaagcct gccagactgg caaaaaaacg cggataagga accagttgtt
     8281 cgcgttcacg tttatcgagc gtgatggttg gaaccagcga ccagaaggga atatccataa
     8341 tggtgtaagt catgccccag aggatgtagg tcacgcaaac aaagacaatc tgagtagtac
     8401 cttcaaacag atgcgcacta aagaggagaa ataagattac agagtttgcc aacgtaccga
     8461 tcaggatcca gggtttgaac ttaccccatc gcgatcgcgt agcatttaca atccatccca
     8521 taatcggatc gttaatagca tcccagatcc tcgccaccag aaacaaagta ccgaccaaac
     8581 ccacagacag cccgacgaca tcggtgtagt aatacatgag gtacatatac acaatgccga
     8641 tcgcaaaatc cttcccgaac gctccaaatc cataactgag ttttgtagtc attgaaatgc
     8701 tcatagggta tcgggtcgct gttaccaacg cccgccttct gttattggta tcgtcagagg
     8761 tgtgcgccgg aataaatccc ccggcgcagt agcgtttagt cgcgttttaa cggtgcaacc
     8821 agcttggcag ccagtcgccg tgggcggcaa tcaggtcgtc aacaagagca tatatttcgt
     8881 caatgcccag cacggcggca gtatgcgggt ccatcatcgc ggcgtggtaa acacggtcgc
     8941 gattttccgt aagaatagct tcggtcagca gcgtctgtac gttgatgttg gtttgcatca
     9001 gggcggccag atgcgaaggt agcgtaccga ctttggtcgg ctgaatgcca ttagcatcaa
     9061 ccagacaggc tacttccacg caacatcctt gtggcaggtt atcaatcaaa ccatcgttac
     9121 ggacgttgcc gtaaatcaca ctcggctcgc cagtccagat agcgttcatg attgtgctgg
     9181 catattcccg tgacggttta atatcaatcc gggaggcgtt tttatactcc tccagctctt
     9241 tatgccagtt cgccagctgc tcgacgcagc gtttcgggta ctcatccagc ggtactttat
     9301 aacgctcaat caaatcctca cgacctagct taataaacca cggcgtatat tcggcaaaat
     9361 gttctgatga ttccgtgacg aagtagccca gctttttgaa catttcgtag cgcacaatat
     9421 tctggcagcg agtattacca tgaatatttg gcttcggtgc ctgccctgct tcataagccg
     9481 ccagcagttc cgggtagaga ttcacgtaac tgccgtcggc ggttttgcgc tccagctcca
     9541 ggtaaaacgc catatggttg atacctgcgc aacggtaacg cagcgtagcc gggtcgatat
     9601 tgaggtcacg cgccagctct tccgccgttc cctgcaccga atggcacagc ccgacctgtt
     9661 tgatatacgg atagcgggca tacatcgccc aggtattcat cgccattggg ttaacatagt
     9721 tgagcatggt ggcatcgggg cagacttccg tcatgttctc gcaaatttgc cacagatgcg
     9781 gaatggtacg tagcgcgcgc ataataccgc ccggccccaa cgtatcggca atggtttgtt
     9841 ccagaccatg ccgcttacag acctcgaaat cagtcaccgt gcaaggttca taaccgccaa
     9901 tctgaaatgc caccacgaca aaatcggcat cctctaaggc ttctttctgt tgggtgtggc
     9961 aggtgatttt gccgctggcc cctgctgaat ccatcagctt acgcaccaca atatgcgact
    10021 cttccaggcg ggtggggtca atgtccatca gggcaatatg cgccgttttc agcgcctcgc
    10081 gatggaacac atcaccaaga atatttttaa cgaaaatcgt cgaaccagcg ccgataaatg
    10141 taattttggg tgcagacatc atgcagatct cctggcttgc ttgaataact tcatcatggc
    10201 aggcgaatct gcagaaaacc tccggcttcc aggttgagca ttcccataaa ctcagatcct
    10261 gcgtgaagca gcagtaaatc tgagtttatg ggaataaatt acctgaaaag tagagttttc
    10321 ggcctcatga tgctcggaca gtcccctcgc ccctctggga agagggtgaa aaccgtgctc
    10381 ccactcgcag tcatcctccc tcactcctgc cataattctg atattctagg aaagagagcc
    10441 atccatgaat acagatacgt ttatgtgcag cagcgacgaa aaacagaccc gcagcccgct
    10501 gtcgctgtat tcggaatacc agcggatgga gattgagttt cgcgcaccgc atatcatgcc
    10561 caccagccac tggcatggtc aggtcgaagt gaatgtgcct ttcgatggcg atgtggaata
    10621 cctgatcaac aatgaaaaag tgaatatcaa tcagggtcat atcacgctgt tctgggcctg
    10681 tacaccgcac caactaacag ataccggaac ctgtcagagc atggcgattt ttaatctgcc
    10741 gatgcatctg tttctctcct ggccgctgga taaagacctg attaaccacg tcactcacgg
    10801 catggtgatc aaatcacttg ccacacagca acttagcccg tttgaagtac gccgctggca
    10861 gcaggaatta aacagtccga atgagcaaat tcgccagctc gccattgatg aaattggcct
    10921 gatgctcaag cgatttagcc tctctagctg ggaaccgatt ctggtcaata aaacctcgcg
    10981 cacacacaaa aacagcgtct cgcgccatgc gcaattttat gttagccaga tgctgggctt
    11041 tattgccgaa aactatgatc aggcgctgac catcaacgat gtggctgagc acgtcaaact
    11101 taacgccaac tatgcaatgg ggatattcca gcgggtcatg caattgacga tgaaacagta
    11161 cattaccgcg atgcgcatca accacgttcg cgcgttactg agcgataccg ataaaagtat
    11221 tctcgatatt gccctgacgg caggctttcg ttcaagtagt cgtttttaca gcacgttcgg
    11281 caaatatgtc ggcatgtcgc cgcaacaata ccgcaaactt agccaacagc gccgccagac
    11341 gtttcccggc taaagacgat atcagtatca gccaaaaaaa tagtttagcc gtcgataata
    11401 agcagtgaaa ataatttaaa ataatcacat aacgtattgt ttataaaact aaatatttca
    11461 aaaatgtttg tttttcacgc gctttacagc ccgaaaaggc cggaagatac ttgcccgcaa
    11521 cgaagattcc ttcataaccg ggtaagcaat gatgaaagta ttaattgttg aaagcgagtt
    11581 tctccatcaa gacacctggg tcggtaacgc cgttgagcgt ctggcagatg ctttaagcca
    11641 gcaaaatgtt accgtgatta aatccacctc ctttgatgat ggttttgcca ttctctcttc
    11701 aaacgaagcc attgactgcc tgatgttcag ctatcaaatg gagcatccgg acgaacatca
    11761 aaacgtcaga caattgatcg gtaagcttca tgagcgccaa caaaacgtgc cggtcttcct
    11821 gttgggcgat cgggaaaaag ccctcgccgc aatggaccgg gatctgctgg agcttgtcga
    11881 tgaattcgcc tggattctgg aagataccgc cgactttatc gccggacgcg ccgttgccgc
    11941 gatgacccgc taccgccagc agctgttgcc gccactgttc agcgcgctga tgaaatatag
    12001 tgacatccat gaatattcct gggcagcgcc aggccaccag ggcggcgttg gttttaccaa
    12061 aacacccgcc ggacgtttct accatgacta ctatggtgaa aatctgttcc gtaccgacat
    12121 gggcatcgaa cgaacttccc tcggttcttt gcttgaccat actggcgcat ttggcgaaag
    12181 cgaaaaatat gccgcacgcg tatttggtgc cgatcgctcc tggtcggtag tcgtcggtac
    12241 ttccggctct aaccgcacca tcatgcaggc ttgcatgacc gataacgatg tcgtggtcgt
    12301 tgaccgtaac tgccataaat ccatcgaaca aggtttgatg ctgacaggcg cgaaaccggt
    12361 ctatatggtg ccaagccgca accgctacgg cattatcggg ccaatctatc cgcaggaaat
    12421 gcaacctgaa accttgcaaa agaaaatcag tgaaagcccg ctgaccaaag acaaagccgg
    12481 gcaaaaaccg tcttactgcg tggtaaccaa ctgcacctat gacggcgtgt gttataacgc
    12541 taaagaagcg caggatctgc tggaaaaaac ctccgatcgt ctgcactttg acgaagcctg
    12601 gtacggctat gcacgtttca acccgatcta tgccgatcac tatgccatgc gcggcgaacc
    12661 tggcgatcac aacggtccta ccgttttcgc cacccactcc atccacaaac tgctgaatgc
    12721 gctgtcacag gcttcttata ttcatgtacg tgaaggtcgt ggggcgatta acttctcccg
    12781 cttcaaccag gcctacatga tgcatgccac cacctccccg ctgtatgcca tctgcgcatc
    12841 caacgacgtg gcggtgtcga tgatggacgg caacagcggc ctgtcactga cacaggaagt
    12901 gattgacgaa gcggttgatt tccgtcaggc gatggcgcgg ctatataaag agttcaccgc
    12961 tgacggtagc tggttcttca aaccgtggaa caaagaagtc gtcaccgacc cacaaaccgg
    13021 caaaacctat gactttgctg acgcaccaac caaactgctg accaccgttc aggactgctg
    13081 ggtaatgcat ccgggcgaaa gctggcacgg cttcaaagat attccggata actggagtat
    13141 gctcgaccca attaaagtca gcatccttgc tccgggaatg ggtgaagatg gtgaactgga
    13201 agaaaccggt gttccggcgg cgctggtcac tgcctggctt ggtcgccacg gcattgtgcc
    13261 tacccgcacc actgacttcc aaattatgtt cctgttctct atgggcgtaa cccgtgggaa
    13321 atgggggact ctggttaaca ccctttgctc cttcaaacac cactatgacg ccaacacacc
    13381 gctggcgcag gtgatgccgg aacttgttga acaatatcct gacacttacg cgaacatggg
    13441 gattcacgat ctgggtgaca ccatgtttgc ctggctgaaa gaaaacaacc ctggcgcacg
    13501 gttgaacgaa gcctattccg gcctgccggt ggcggaaatc accccgcgtg aagcgtacaa
    13561 cgcgattgtc gacaacaatg tcgaactggt atccattgaa aatctgccag gacgcatcgc
    13621 ggcaaactca gttatcccgt atccgccagg aatcccgatg ctgctgtctg gtgaaaactt
    13681 cggcgataaa aacagtccgc aagtaagtta tttacgctcg ctgcaatcct gggaccacca
    13741 tttccctaga tttgaacacg aaactgaagg gactgaaatt attgacggta tttaccacgt
    13801 tatgtgcgtg aaagcgtaac cactattccg ctgaaggcgt aattgtttaa ataacattac
    13861 gccgcctggc cttaggcctt ttgagtatgg caacgttttc ataaaaattg ctgcaaacaa
    13921 aaatgtcata ctttttgcgc ggccccaccc cacgcttttt tttgcctgtt atttatcctg
    13981 taaaaaaata tgtacatgag aaattactat aaaaatttgt aatattagta aaactcgtta
    14041 tttttatgca tgtttatatt catcatgcaa ttatataacc atttcccggt atcgctttgc
    14101 tttagcgaga actggtcttt tattcgcact caggagtaca tgtatgagga tttgcagcga
    14161 ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga atgggaaaga
    14221 acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata atattatcga
    14281 cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca tcaaagatta
    14341 cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta gcgctacgcc
    14401 catactcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc aacacagcat
    14461 gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat tcacggtgct
    14521 atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt tacgtaattg
    14581 tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact ggaatcttag
    14641 tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagt tgaaaagcga
    14701 aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg taaatgaatt
    14761 aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca acagtacgtc
    14821 gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt atgttagtca
    14881 gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata ttaataacgg
    14941 catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta tgatatgacg
    15001 taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg gatgctgatg
    15061 ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt atggggtcag
    15121 gtgtttttct gttacctgca aacctggcct ccactggcgg gattgccatt tatggatggt
    15181 tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg tcgttcctcg
    15241 acccaagtcc tggtggttct tacgcttacg cccgccgctg ctttggcccg tttctcggtt
    15301 atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgcc atggtggtca
    15361 ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg gtattaacca
    15421 tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt ccgaaaatga
    15481 tcacccgcgt gcaggccgtt gccactgtgc tggcgctgat ccccatcgtc gggattgccg
    15541 tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac gtcagcggcc
    15601 tgggcacctt cggtgcgatt caaagtaccc ttaacgttac gctgtggtcg ttcatcggtg
    15661 tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaat gtccctatcg
    15721 ccaccattgg tggggtattg attgccgccg tttgctatgt gctctccacc accgcgatta
    15781 tggggatgat ccctaatgcc gcactgcgcg tttctgcctc gccattcggt gatgccgcac
    15841 ggatggcgtt gggtgacacc gctggggcca ttgtttcctt ctgcgcagct gcgggttgct
    15901 taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc gctgccgatg
    15961 acggactgtt cccaccgatt tttgcccgtg taaataaagc gggtacgcca gtggcggggc
    16021 tgattatcgt cggtattttg atgaccatct tccagctcag cagcatttca ccaaacgcga
    16081 ccaaagagtt cggtctggtt tcttccgtct cggtcatctt tacactggtg ccatatcttt
    16141 acacctgtgc ggcgttactg ctgctcggcc acggtcactt tggtaaagca cgcccggcat
    16201 atctggctgt tactaccatt gccttcctct actgcatttg ggccgtggtg gggtccgggg
    16261 cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat caccgccatg tatgcgctga
    16321 attacaaccg gctacataaa aacccgtatc ccttagatgc accaataagc aaagattaat
    16381 tccccttaat ccagcaaaca taaaagccaa ccttaagaac ttaaggttgg cttaattttg
    16441 ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa gcgcctacta
    16501 aaaagaccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta tatctctatc
    16561 tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga ttcgctgcat
    16621 aacgtactgg ttttcttgtc gatgccggtt gtcgctttca gcgtgattaa tattgtcctg
    16681 acactaagct ctttcttatg gcttaatcga ccactggcct gcctgtttat tctgattggc
    16741 gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc gatgattgcc
    16801 aatattattg ataccactcc agcagaaagt tatgcgctga tgacaccgca aatgttatta
    16861 acgctgggat tcagcggcgt gcttgctgag ctgattgcct gctggctaaa aatcaaacct
    16921 gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt ttctgtactg
    16981 ctgattttgc tggtcgccgc actgttttat aaagactacg cctcgttgtt ccgcaataac
    17041 aaagagctgg tgaaatcctt aagcccctct aacagcattg ttgccagctg gtcatggtac
    17101 tcccatcagc gactggcaaa tctgccgctg gtgcgaattg gtgaagacgc gcaccgcaac
    17161 ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg cgaaacctcg
    17221 cgggcggaga acttctccct caacggctac ccgcgtgaaa ctaacccgcg gctggcgaaa
    17281 gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc agtttcagta
    17341 ccgtgcatgt tctcggatat gccgcgtgag cactacaaag aagagctggc acagcaccag
    17401 gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa tgacaacgat
    17461 ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc gctgaatctg
    17521 cctgatcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg gctggaagag
    17581 tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg cagccacggt
    17641 ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac ctgcgacacc
    17701 aatgagatcc agacctgtac caaagagcaa ctggtgaaca cttacgacaa cacgctggtt
    17761 tacgtcgact atattgttga taaagcgatt aatctgctta aagaacatca ggataaattt
    17821 accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa tggcatctat
    17881 ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc aatgctgctg
    17941 tggctgtcgg aggattatca aaagcggtat caggttgacc agaactgcct gcaaaaacag
    18001 gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg attaactggc
    18061 gttgagacga agtattacca ggccgcggat gatattctgc aaacttgcag gagagtgagt
    18121 aaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggactg attctggcgg
    18181 cgcaaaccga aggctacgcg tgcgatggcg tgacaaccgc gcggatggcg gaacaaagcc
    18241 ttgaggacgg tcattacagc ctggtggtac tggatttagg gttacccgac gaagatggac
    18301 tgcattttct cgcccgtatc cggcagaaaa aatacaccct gccggtactg atcctcaccg
    18361 ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt cggtgccgat gactatctgg
    18421 tgaagccttt tgcgctggaa gagttacacg cccgtatccg cgccctgcta cgacgccata
    18481 ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaatatg ggtcgccgtc
    18541 aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct ctgctgtcac
    18601 ggttaatgct caaagcaggc agtccggtac atcgggaaat tctctacaac gacatctata
    18661 actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat ctgcgcgaca
    18721 aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctg gtcgcgaatg
    18781 aggaaaacta attgaatctg atgcgttttc tgcgccgacc tatatcgctg cgccaacggc
    18841 tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc ttctggctat
    18901 ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac aatcgcaaca
    18961 acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt gtccccggcg
    19021 tctttatggt cagcctgacg ctatttatct gctatcaggc ggtacgccgc atcacccgcc
    19081 cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc cgacaactta acgcccattg
    19141 ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttgaac gatctggtca
    19201 gtcgcctgac cagcacgctg gataacgaaa ggttgtttac cgctgacgtc gcgcacgaac
    19261 tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa acgcatcaca
    19321 ttgatgtagc accgttagtg gcacggcttg atcagatgat ggagagcgtc tcccagctgc
    19381 tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat gtaaaactgc
    19441 tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac cagcgacagc
    19501 aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat gcaaccctgc
    19561 tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagcccg caaggcagca
    19621 acattatgat taagctgcaa gaagatggcg gagcggtcat ggcagttgaa gatgaaggac
    19681 caggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt atggacagcc
    19741 gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag ttgcatcacg
    19801 ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg gtacggctga
    19861 agaaagatca gaacgtggca aaccagatat aaagaaagct gctgaccacc agcacgctga
    19921 acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc gttaacctgg
    19981 aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc ctgataagac
    20041 agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt tgctgcacca
    20101 ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatattatcg attttctgct
    20161 cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct gacgccgccg
    20221 gtgtcgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg gtgattaaac
    20281 caatcaccgc cactaccatc aggtaatagg caggcatcat cagattctgc gagctttcga
    20341 ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata ttaaatgccg
    20401 ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta gaagccataa
    20461 cgcccataaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa atcaggccga
    20521 tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagt gcaacactac
    20581 caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc accggctgga
    20641 caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct tcggagtaat
    20701 gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa gtcacgttgg
    20761 tggcaattac cagaccaata catgtcaaca ggctgcgcca gtatttagtg gcaatctctt
    20821 taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc agtttatcaa
    20881 catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc ccgataatcc
    20941 ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag ttcgcttcgc
    21001 cgacaatggt cgaaattaac accaccacgc ccgcacccag cacaaacccg gcaatagaac
    21061 cgaagtccag ccagctgccc ataaagccac gtttacggtc aggggagtat tccgcaacaa
    21121 atatcgacgc cccggtatat tcaccaccga ccgagaaacc ttgtgccatc ttacagatca
    21181 acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag ccaatacaga
    21241 acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga ccatatttat
    21301 cgcccaacat accaaagaag agtccgccaa gcggtcgaat cagaaaggga acggagaaag
    21361 tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa actttaccta
    21421 atgcgtaagc aacaaaacca taaacaccaa aatcaaacca ttccattgca ttacccagtg
    21481 atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca tcacgaaggg
    21541 taatcggttt tacttttttc cttttcagca tagctttcct cgcagagttg gcccatttcc
    21601 gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt gcaggatgca
    21661 atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat gtacaatcaa
    21721 accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt gtttaaattt
    21781 gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga ttatttacgc
    21841 caaatttaca gagaagattt cctcgaccat tgcccccacc aaacgctgcg ctgaacagag
    21901 tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc actgaccagc aaacgggttg
    21961 tttatctgcc gtggtccact ttcccagcca acttaaaaaa tcagcatgat ccccgcccgt
    22021 cggaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtagtcg gtgccgcatt
    22081 gatactctct gccagatgtt gtgtcgcttt ctcgcgcaaa tcacccagca acaacaccac
    22141 cgcttgcggg cttcccagac ggcaggcatc gccaaatgca ccccagcgca actcctccag
    22201 aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt
    22261 aaaaatttgc tcgctctgat cacctgcctg caccagtagg cctcgttgcg ttaacgcctt
    22321 ttccttccag cgacaacggg ctgaaaacca ctcatacaag gcggcaattt gctgggggag
    22381 ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca gatgctgcgc
    22441 gatctgcgtc agcgccagat gaccgggatc cagcatcgac gccagttttt ccaccattgt
    22501 cagtcgcagg ctaagcggcg catttaacat gccgccgttc gtccatgtct gaagctgcac
    22561 acgcgaaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg cttgaggatg
    22621 gcgggcatca tcgccctgcg ccagatccac taaaaagcgc ggatgaaccg actccagcac
    22681 gcgaccgggg ccgtccagta acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga
    22741 atatcatcgc gtaacagctg agcgtcggtg taaatccagc tgtgagtcat cacagtctgc
    22801 tgcaattgct gctgcatcag cctgaccacc gattcatttt gttgacgcag agccagactt
    22861 tcgcgtaaac gcgtctgtaa ttctgtcaaa catgaagcga actcagcgaa aaaagtattc
    22921 atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat ttgttggcaa
    22981 tagagatcga cttctgcgct taatgctcgt tgcaacacac tgtaatcaac cgtttctgtc
    23041 ggggatttct catttccccg tccccagtcg ggctgattca accagcgcga aaaagtctca
    23101 cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt cctggcgcga aatgacttca
    23161 ctgaacagct ggcgagtgtt gaagtgggga actacgccgt gaaaaacagg aaaatgaaac
    23221 ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat ggggcgaata
    23281 acatcggtta acacccggca aagggtggat tccatctcgg caaaacgcag cgtaaagtcg
    23341 cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcgggtacg aatctcgctt
    23401 aacggctccg aatcatcctg aaacaaggcg gctaactgcg cattcaccgc atcttgctgt
    23461 tgacgcagaa agtggttggc ggaggtcagg gccagctcga tttcatgttt aatctcgccg
    23521 ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg atggatattt
    23581 tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc
    23641 gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc gcagagcata caatgaggcg
    23701 ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac
    23761 aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg acgccagcgc
    23821 ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cgctggtaac
    23881 ttaccgttgt tggctaactc atagcgcgcc cgattcgcca ggtagcccca catcgacgac
    23941 accgggaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga aatcagtgcc
    24001 cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca
    24061 tacagcggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc atcggagatc
    24121 gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg
    24181 ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt atccagtaac
    24241 gtcaactgac cgggataact ttccagcccc gccagatgga caaactccac ttcaatcacg
    24301 ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc
    24361 agtcgcacta aatcattcaa acttttcaga caatgaaaaa taggctgggc acccagataa
    24421 tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat atctttatct
    24481 atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt
    24541 tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca gtaccggttc cttttgcccg
    24601 ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattacgatt aggcagaacc
    24661 tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatggt accgacaatt
    24721 gccagcacca tttccagtcg ggaaatttta cacaactcat tattcagcat cgcgtgacgt
    24781 tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcgtcgtc tccgggtaca
    24841 gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa caggcgttca
    24901 gcctcctgac ttaactcata cagggtctgt gtgtacatag aaaattcttc cttaaagcaa
    24961 attttgttac ttttatttag ccagattgtt tttgagttct gttttcggct tttataatta
    25021 ctgcaagaaa taattttata tttagtgtgt tgttttttta tcagaataaa taacgtcttc
    25081 tgatgcgttt aaaacgtcag aaagataaaa atatcatgtg aataaaaaaa agaacaagta
    25141 gagcattaac attatcttaa ataataaata gaggcaaaaa gattattttc tttttgcgtt
    25201 tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt acccccgaca aactcaggga
    25261 ttttgtgtat aattgcggcc tttttcggca atctgccgtt ttttggcgct tttgccctgc
    25321 tgacttttga ggaaatccac atgtcattac cacactgccc aaaatgcaac tccgaataca
    25381 cttacgaaga taacggcatg tacatctgcc cggaatgtgc ctacgaatgg aacgacgcag
    25441 aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc taacggcaat ctgctggctg
    25501 acggcgacag cgttaccatc attaaagatc tgaaggtgaa aggtagctct tcgatgctga
    25561 aaattggcac caaagtgaaa aacatccgcc tggttgaagg cgaccataac atcgattgca
    25621 aaatcgacgg ttttggtccg atgaaactga aatctgagtt tgtgaaaaag aactgattgt
    25681 attgtgatcg gtaagccgga taaggcgctc gcgccgcatc cggcaacgat gccgactgcc
    25741 tgatgcgacg cttgcgcgtc ttatcaggca gcctctcatc tgtataaatt tcgaactaca
    25801 cttaactggc ttctcttaac tgaggtcacc atcatgccgt taagtcccta cctctctttt
    25861 gccggtaact gttccgacgc gattgcctat tatcaacgta cgttgggcgc ggaactgctc
    25921 tataaaatca gcttcggcga aatgccgaaa tcagcgcagg acagcgccga gaactgccct
    25981 tccggaatgc aatttcccga taccgccatc gctcatgcca acgtgcgcat tgccggaagc
    26041 gacatcatga tgagcgatgc tataccgtca ggaaaagcca gctactccgg ctttacgctg
    26101 gtgctcgatt cgcaacaggt cgaagaagga aaacgctggt ttgataacct tgccgctaac
    26161 ggaaaaatcg aaatggcctg gcaggaaact ttctgggcgc atggctttgg caaagtcacc
    26221 gataaatttg gcgtaccgtg gatgattaat gtcgtcaaac aacaaccaac gcaataaccc
    26281 gccgggaggc ccgccctccc gcactgtcat cgaattcccg ttaactcttc atctgttagt
    26341 cacttttaat taaccaaatc gtcacaataa tccgccacga tggagccact tttttaggga
    26401 ggctgcatca tgcaaacgat tatccgtgtc gagaagctcg ccaaaacctt caatcagcat
    26461 caagcgctgc atgcggttga tctgaacatt catcacagtg aaatggtggc tctgcttggg
    26521 ccgtcgggtt ccggcaaatc caccctttta cgtcatttaa gcggtttgat taccggcgat
    26581 aaatccgccg gtagccatat cgagctactg ggccgcactg tccagcgcga aggccgcctg
    26641 gcccgcgata tccgcaaaag ccgcgccaac accggctaca tctttcaaca attcaacctg
    26701 gtgaaccgcc tgagcgtact ggagaacgtg ctgattggcg cgctcggcag cacgccgttc
    26761 tggcgcacct gttttagctg gttcaccggc gagcagaaac agcgcgcgtt acaggcgctg
    26821 acccgcgttg gcatggtgca ttttgcccat cagcgcgttt ccaccctctc cggcggccag
    26881 cagcaacgtg tggcgattgc ccgtgcgctg atgcagcagg cgaaagtgat tctggccgat
    26941 gaacccatcg cctcgctgga cccggaatca gcgcgcatcg tgatggacac cctgcgcgac
    27001 atcaaccaga acgacggcat caccgtggtc gtcacgctgc atcaggtgga ttacgccctg
    27061 cgctactgcg aacgcatcgt cgccctgcgt caggggcacg tcttctacga cggcagcagc
    27121 caactgtttg ataacgaacg ttttgaccat ctctaccgca gcattaaccg catcgaagag
    27181 aacgcgaaag ctgcctgata tccccatcat tgaggaaaac gaatgaacgc taagataatt
    27241 gcctcgctgg ccttcaccag catgttcagc ctcagcaccc tgttaacccc ggcacacgcc
    27301 gaagagcagg aaaaggcgct gaatttcggc attatttcaa cggaatcaca gcaaaacctg
    27361 aaaccgcaat ggacgccgtt tttacaggat atggagaaga aactgggcgt gaaggtcaac
    27421 gccttctttg ccccggacta cgcgggcatt atccagggga tgcgcttcaa taaagtggat
    27481 attgcctggt acggcaacct gtcggcgatg gaagcggtgg atcgcgccaa tggccaggtc
    27541 ttcgcccaga cggtcgcggc ggatggatcg ccgggttact ggagcgtgtt gatcgtcaac
    27601 aaagacagcc cgatcaacaa cctgaacgat ctgctggcga agcggaaaga tctcaccttc
    27661 ggcaatggcg atcctaactc cacctctggc ttcctcgtcc ccggttacta cgtcttcgct
    27721 aaaaacaata tctccgccag cgacttcaag cgcaccgtca acgccgggca tgaaaccaac
    27781 gcgctggccg tcgccaacaa gcaagtggat gttgccacca acaacaccga aaacctcgac
    27841 aagctgaaaa cctccgcgcc ggagaagctt aaagagctga aggtgatctg gaaatcgccg
    27901 ctgatcctag gcgatccgat cgtctggcgc aagaatctgt ccgaaactac caaagacaag
    27961 atctacgact tctttatgaa ctacggaaaa acgccggaag agaaagcggt gctggaacgc
    28021 ctgggctggg cgccgttccg cgcttccagc gacctgcaac tggtgccgat tcgccagctc
    28081 gcactgttta aagagataca aagcgtgaaa ggcaataaag ggctgaatga gcaggacaag
    28141 ctggcgaaaa ccaccgagat tcaggcgcag ctggatgacc tggaccgcct gaacaacgcg
    28201 ttaagcgcga tgagttcggt gagtaaagcg gtgcagtaaa tcgtaggtcg gataagacgc
    28261 cccggcgtcg catccgacaa tgtgtaggcg ttgatgccgg atgcggtgca agcaccttat
    28321 ccggcctaca gaccggagcc aaacatgcaa accatcacca tcgccccacc caagcgtagc
    28381 tggttctcgc ttctgagctg ggccgttgtt ctcgacgtgc tggtcgtctc gtggcagggc
    28441 gcggaaatgg ccccgcttac gctgattaaa gacggcggca acatggcaac ctt
//
LOCUS       DAJTNP010000049        28360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTNP010000049 DAJTNP010000000
VERSION     DAJTNP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            564..1334
                     /locus_tag="OO160_002272"
     CDS             564..1334
                     /locus_tag="OO160_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5524374.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO160_002273"
     CDS             1325..1798
                     /locus_tag="OO160_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5524375.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO160_002274"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO160_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5524376.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO160_002275"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO160_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5524377.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO160_002276"
     CDS             3233..3727
                     /locus_tag="OO160_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5524378.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO160_002277"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO160_002277"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5524379.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO160_002278"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO160_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO160_002279"
     CDS             7557..8471
                     /locus_tag="OO160_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5524380.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO160_002280"
     CDS             8686..10047
                     /locus_tag="OO160_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524381.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO160_002281"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO160_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5524382.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO160_002282"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO160_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5524383.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO160_002283"
     CDS             12597..13886
                     /locus_tag="OO160_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5524384.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO160_002284"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO160_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5524385.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO160_002285"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO160_002285"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5524386.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO160_002286"
     CDS             16212..16592
                     /locus_tag="OO160_002286"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5524387.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO160_002287"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO160_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5524388.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO160_002288"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO160_002288"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5524389.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO160_002289"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO160_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5524390.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO160_002290"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO160_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5524391.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO160_002291"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO160_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO160_002292"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO160_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO160_002293"
     CDS             23321..23833
                     /locus_tag="OO160_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5524392.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO160_002294"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO160_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5524393.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO160_002295"
     CDS             26426..26629
                     /locus_tag="OO160_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5524394.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO160_002296"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO160_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5524395.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>28360)
                     /locus_tag="OO160_002297"
     CDS             complement(27665..>28360)
                     /locus_tag="OO160_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5524396.1"
                     /translation="LSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMH
                     GWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPN
                     QVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLH
                     KIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMRH
                     EKMKTQLKKLWLV"
BASE COUNT         6937 a         7416 c         7117 g         6890 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agctatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattggatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca agcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcctgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcat tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcttgagga agaatatgct
    27961 caattgtgat tctccccgta taggttttaa tcacgctctc atcttgttgc tccatatcta
    28021 agcgaagcaa aattgctttt attaagttca cttgattcgg acgaggctca tataaatctt
    28081 catccagagc agctataaaa cctgaattat ctgcatgttg gtttatctga tttatcacag
    28141 aatcaaaagg catatcatta tttattgcta ccagagcaga ataacaaacc atttctcttt
    28201 ggcttttaat ttgttttttt aaccacccat gcatatatac cttctcaaag gcggtaataa
    28261 attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc cattcgattc atgaatgcca
    28321 taaccggagg aatccactca tctaccccta aattacttag
//
LOCUS       DAJTNP010000050        28305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTNP010000050 DAJTNP010000000
VERSION     DAJTNP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO160_002298"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO160_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO160_002299"
     CDS             complement(1023..2045)
                     /locus_tag="OO160_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5524397.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO160_002300"
     CDS             complement(2059..3561)
                     /locus_tag="OO160_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5524398.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO160_002301"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO160_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO160_002302"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO160_002302"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5524399.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO160_002303"
     CDS             complement(5877..6218)
                     /locus_tag="OO160_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5524400.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO160_002304"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO160_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5524401.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO160_002305"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO160_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5524402.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO160_002306"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO160_002306"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5524403.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO160_002307"
     CDS             12897..14240
                     /locus_tag="OO160_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5524404.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO160_002308"
     CDS             complement(14302..14508)
                     /locus_tag="OO160_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5524405.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO160_002309"
     CDS             14833..15387
                     /locus_tag="OO160_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5524406.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO160_002310"
     CDS             complement(15450..16388)
                     /locus_tag="OO160_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524407.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO160_002311"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO160_002311"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5524408.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO160_002312"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO160_002312"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5524409.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO160_002313"
     CDS             complement(19599..19979)
                     /locus_tag="OO160_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5524410.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO160_002314"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO160_002314"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5524411.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO160_002315"
     CDS             21037..22002
                     /locus_tag="OO160_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5524412.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO160_002316"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO160_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5524413.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO160_002317"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO160_002317"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5524414.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO160_002318"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO160_002318"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5524415.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO160_002319"
     CDS             25376..25921
                     /locus_tag="OO160_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5524416.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO160_002320"
     CDS             complement(25905..26552)
                     /locus_tag="OO160_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5524417.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO160_002321"
     CDS             26764..27555
                     /locus_tag="OO160_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5524418.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28305
                     /locus_tag="OO160_002322"
     CDS             27565..>28305
                     /locus_tag="OO160_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5524419.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGF"
BASE COUNT         6941 a         7541 c         6978 g         6845 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tataaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gattt
//
LOCUS       DAJTNP010000051        27524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTNP010000051 DAJTNP010000000
VERSION     DAJTNP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO160_002323"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO160_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5524420.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO160_002324"
     CDS             complement(1614..1937)
                     /locus_tag="OO160_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5524421.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO160_002325"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO160_002325"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5524422.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO160_002326"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO160_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5524423.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO160_002327"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO160_002327"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5524424.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO160_002328"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO160_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5524425.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO160_002329"
     CDS             7537..8574
                     /locus_tag="OO160_002329"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5524426.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO160_002330"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO160_002330"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5524427.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO160_002331"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO160_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5524428.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO160_002332"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO160_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5524429.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO160_002333"
     CDS             10745..11626
                     /locus_tag="OO160_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5524430.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO160_002334"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO160_002334"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5524431.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO160_002335"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO160_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5524432.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO160_002336"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO160_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO160_002337"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO160_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5524433.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO160_002338"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO160_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5524434.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO160_002339"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO160_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5524435.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO160_002340"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO160_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5524436.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO160_002341"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO160_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5524437.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO160_002342"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO160_002342"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5524438.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO160_002343"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO160_002343"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5524439.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO160_002344"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO160_002344"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5524440.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO160_002345"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO160_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5524441.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO160_002346"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO160_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5524442.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO160_002347"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO160_002347"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5524443.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO160_002348"
     CDS             24893..25273
                     /locus_tag="OO160_002348"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5524444.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO160_002349"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO160_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5524445.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO160_002350"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO160_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5524446.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27524
                     /locus_tag="OO160_002351"
                     /pseudo
     CDS             26442..27524
                     /locus_tag="OO160_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6788 a         6850 c         7217 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccgg
//
LOCUS       DAJTNP010000052        26553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTNP010000052 DAJTNP010000000
VERSION     DAJTNP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            337..630
                     /locus_tag="OO160_002352"
     CDS             337..630
                     /locus_tag="OO160_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5524447.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            702..1361
                     /locus_tag="OO160_002353"
     CDS             702..1361
                     /locus_tag="OO160_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5524448.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1453..1899
                     /locus_tag="OO160_002354"
     CDS             1453..1899
                     /locus_tag="OO160_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5524449.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2106..3006)
                     /gene="hlyE"
                     /locus_tag="OO160_002355"
                     /pseudo
     CDS             complement(2106..3006)
                     /gene="hlyE"
                     /locus_tag="OO160_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3379..3798
                     /locus_tag="OO160_002356"
     CDS             3379..3798
                     /locus_tag="OO160_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5524450.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3798..5066
                     /gene="umuC"
                     /locus_tag="OO160_002357"
     CDS             3798..5066
                     /gene="umuC"
                     /locus_tag="OO160_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5524451.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5112..5642)
                     /gene="dsbB"
                     /locus_tag="OO160_002358"
     CDS             complement(5112..5642)
                     /gene="dsbB"
                     /locus_tag="OO160_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5524452.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5788..7329)
                     /gene="nhaB"
                     /locus_tag="OO160_002359"
     CDS             complement(5788..7329)
                     /gene="nhaB"
                     /locus_tag="OO160_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5524453.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7550..8269
                     /gene="fadR"
                     /locus_tag="OO160_002360"
     CDS             7550..8269
                     /gene="fadR"
                     /locus_tag="OO160_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5524454.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8321..9853)
                     /locus_tag="OO160_002361"
     CDS             complement(8321..9853)
                     /locus_tag="OO160_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5524455.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10183..11481
                     /gene="dadA"
                     /locus_tag="OO160_002362"
     CDS             10183..11481
                     /gene="dadA"
                     /locus_tag="OO160_002362"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5524456.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11491..12561
                     /gene="dadX"
                     /locus_tag="OO160_002363"
     CDS             11491..12561
                     /gene="dadX"
                     /locus_tag="OO160_002363"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5524457.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12729..14143)
                     /locus_tag="OO160_002364"
                     /pseudo
     CDS             complement(12729..14143)
                     /locus_tag="OO160_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14754..16490)
                     /locus_tag="OO160_002365"
     CDS             complement(14754..16490)
                     /locus_tag="OO160_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5524458.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16585..17499)
                     /gene="ldcA"
                     /locus_tag="OO160_002366"
     CDS             complement(16585..17499)
                     /gene="ldcA"
                     /locus_tag="OO160_002366"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5524459.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17599..18210
                     /gene="emtA"
                     /locus_tag="OO160_002367"
     CDS             17599..18210
                     /gene="emtA"
                     /locus_tag="OO160_002367"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5524460.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18212..18946)
                     /locus_tag="OO160_002368"
     CDS             complement(18212..18946)
                     /locus_tag="OO160_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5524461.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19147..19401
                     /locus_tag="OO160_002369"
     CDS             19147..19401
                     /locus_tag="OO160_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5524462.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19451..20911)
                     /locus_tag="OO160_002370"
     CDS             complement(19451..20911)
                     /locus_tag="OO160_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5524463.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20848..21405)
                     /locus_tag="OO160_002371"
     CDS             complement(20848..21405)
                     /locus_tag="OO160_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5524464.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21431..22285)
                     /locus_tag="OO160_002372"
     CDS             complement(21431..22285)
                     /locus_tag="OO160_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5524465.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22285..23094)
                     /locus_tag="OO160_002373"
     CDS             complement(22285..23094)
                     /locus_tag="OO160_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5524466.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23104..23862)
                     /locus_tag="OO160_002374"
     CDS             complement(23104..23862)
                     /locus_tag="OO160_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5524467.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23859..24839)
                     /locus_tag="OO160_002375"
                     /pseudo
     CDS             complement(23859..24839)
                     /locus_tag="OO160_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(24839..25861)
                     /locus_tag="OO160_002376"
     CDS             complement(24839..25861)
                     /locus_tag="OO160_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5524468.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6673 a         6469 c         6852 g         6559 t
ORIGIN      
        1 ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag cagtgcaagc
       61 agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt cagaatgagt
      121 acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc ttttctgcgt
      181 tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag gggcatggac
      241 tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca ttgatgttag
      301 cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgcgat ttatcgaagc
      361 agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc gcgtgttcct
      421 gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc gttggatggg
      481 cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac cgagcaaggt
      541 tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct ttccgtcatg
      601 gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat tatctttgtc
      661 ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa catcacaact
      721 ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc agtaactatg
      781 ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg tttattaaac
      841 cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat ttcggttcag
      901 ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag gcaacggaag
      961 agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca ttgcgtgatg
     1021 ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg tttgataact
     1081 cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca caaaatacaa
     1141 cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg gacatgatcc
     1201 ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag gccggtgacg
     1261 ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat gagctgacag
     1321 tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg ccgccagaaa
     1381 gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt taacgtaatg
     1441 gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga cgaaatgagc
     1501 gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcttgca taaactgatg
     1561 gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct caatattaaa
     1621 acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg cattaaatta
     1681 acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta tcggttgctg
     1741 gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa agcggcaatg
     1801 catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt gattgactgg
     1861 caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata gatgctgatg
     1921 ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt ttgaaagcgc
     1981 tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc aatctttaaa
     2041 aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga gagaataatc
     2101 gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt ctgatactca
     2161 ttacaggtgt taatcatttt tttggccgct gcttttagca aagaaagcat taaatcatca
     2221 taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat ggcggctatt
     2281 tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt aaccgtgtta
     2341 gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc tggaatcagt
     2401 ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa tggaccggcg
     2461 acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac ctgtgactgg
     2521 aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc cagcagtttc
     2581 ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc ttcattcagc
     2641 ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct cattgtactc
     2701 atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc attcatacac
     2761 tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt taatatcgcc
     2821 gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact cttttatggt
     2881 ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa gatctaatgc
     2941 tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg caacgatttc
     3001 agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg ttttacaact
     3061 attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt caatgtcttt
     3121 atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg aatgcttcta
     3181 ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg tcgccagaag
     3241 gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg acaaatattg
     3301 atagcctgaa tcagtattga tctgctggca agaacagacc actgtatata aaaacagtat
     3361 aacttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga aattgtgact
     3421 tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc agattacgtt
     3481 gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac ttacttcgtc
     3541 aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt actgattgtc
     3601 gatagtgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga cggcgagttt
     3661 acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa cagtgcgtac
     3721 tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt gatccacgtc
     3781 gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat gccagctgtg
     3841 agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg aataatgacg
     3901 gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg ggcgatccct
     3961 ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc agcaattatg
     4021 agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta tcgccccgcg
     4081 tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt aattgtcgcg
     4141 atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc catcttactg
     4201 ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat gcggcaaaaa
     4261 aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat
     4321 taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc agcaaaaaac
     4381 tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc cggtttatcc
     4441 gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc gaaccctgtt
     4501 tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc tcgtttggtg
     4561 aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct gcccgggcgg
     4621 cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt attaagacgt
     4681 caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa ctgctgacgc
     4741 ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat gccatctggc
     4801 aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc agtcagggag
     4861 tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag caattgatgg
     4921 cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt gccgggcagg
     4981 ggatccaaca acaatggcag atgaagcgag ctatgctttc accacgttat acaacgcgaa
     5041 gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct cccgcaggag
     5101 cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg ctgggaaatc
     5161 accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca ctgcggcatt
     5221 tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac aaacacttgc
     5281 ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc acaggtggca
     5341 aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac accgcggaac
     5401 gcactataca accagataac catcgctaca taacgcagcg gagttttcgg ggcgatcgcg
     5461 ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat acagagcacg
     5521 caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag tgccagagca
     5581 gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa aaatcgcaac
     5641 ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca ttcgctgcgc
     5701 caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa aggcaaaccg
     5761 ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca tttgcataaa
     5821 ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga gtgtcagtac
     5881 gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa tcaatggcgc
     5941 gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag cgacagaggg
     6001 cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca acgtgatagc
     6061 gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga agacgttatc
     6121 cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag catgttcaga
     6181 tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa tcaccgcgac
     6241 taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga aggctttacc
     6301 gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga ctgacaaacc
     6361 aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga cgccaataat
     6421 cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc tttgatcgtc
     6481 aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc caaaccaacg
     6541 cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa cggtcaccgg
     6601 cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga tgatcaggtt
     6661 ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac cggcatgcat
     6721 catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat gcttgtcgat
     6781 atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc gatgataaat
     6841 accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg catcgaggaa
     6901 cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt tggagcgaat
     6961 gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat agatacccgc
     7021 caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt cacggacgtg
     7081 ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac cggggagtag
     7141 cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca gcaaccagcc
     7201 cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa tgatgagggc
     7261 gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc ggccccagga
     7321 gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta tgtttaaacg
     7381 caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg aaagagatgc
     7441 ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt agttagccct
     7501 ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta tggtcattaa
     7561 ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct ggaataaccg
     7621 cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa ttggcgtaac
     7681 gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt tgaccattca
     7741 acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa atatccttga
     7801 aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt tgctgtcggt
     7861 gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc ccgataaagc
     7921 gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct ttgccgagct
     7981 ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt acggtctgat
     8041 ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg ccaatccgga
     8101 agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca gtgaaggcgc
     8161 gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg agatttggca
     8221 ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat cccttccgtt
     8281 taaagagcaa ccccctcaaa cgaggggttt tttgttgttt ttacagattt cccattcttg
     8341 gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg agcatcacat
     8401 caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga tccagcggtg
     8461 cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa tccacgttcc
     8521 agatctgaat attcggctcc agattactta agttatattg cgacgataac cggttacgga
     8581 tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta tgccgatcat
     8641 catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga aactggctga
     8701 taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc cagtcggaac
     8761 cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa atccgtttaa
     8821 tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac cacgggctgt
     8881 tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc ataaaacgtt
     8941 ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag aaggtcgcac
     9001 agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc accttacgca
     9061 caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc tccataaaat
     9121 agagcaggtt ttcttgtggt tcggacggat agcggcgcgc ttcagcaacc gttttctctt
     9181 cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga tactcttcgc
     9241 gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt ttgtaccggt
     9301 ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact tcatcaacgc
     9361 cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa tcgacaatcg
     9421 aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag ttatgtccat
     9481 agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc atcaagtaag
     9541 cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga ccgtgcttat
     9601 acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag ttaattggca
     9661 tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca atctggtgcg
     9721 ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga taaacatcca
     9781 gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta ttcatagaat
     9841 cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga gcacctcatt
     9901 tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct ttgcgatttc
     9961 ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga taaaaagggc
    10021 gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat gtgagtcagc
    10081 tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa gatgtaatta
    10141 gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt tgtcatactg
    10201 ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg acatgaggtc
    10261 accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa tgccgggcaa
    10321 atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa agcgattaaa
    10381 tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca gtttcagttg
    10441 aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga aaacaaaggg
    10501 cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg cgccgaaacc
    10561 aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac cgaacaacag
    10621 tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc gtatcagctg
    10681 ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc gcacaaactg
    10741 acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt tacccagaat
    10801 ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc cgttgaccaa
    10861 ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt gattaaggcc
    10921 gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg cattgttgat
    10981 attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca ggaagatggt
    11041 gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg tttcgataac
    11101 cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct gttgcaaccg
    11161 cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg tcatgtcgag
    11221 caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc ggttgtcggg
    11281 agtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg ctggacgatg
    11341 gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc agcgatccca
    11401 tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg tccgggccat
    11461 ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc cagcctcgat
    11521 ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca cgcgcgcgtc
    11581 tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg gagcgcgctc
    11641 ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt acgtgagcgc
    11701 ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga tctggagatt
    11761 tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa agcactgcaa
    11821 aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg gatgaatcgg
    11881 ttgggcttcc agtccgatcg cgtgcttacc gtctggcagc agttgcgggc aatggcgaat
    11941 gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga tggaatttcc
    12001 ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg ttcgttgtcc
    12061 aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg gcctggcatt
    12121 attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac cggattacgt
    12181 ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc gggcgagcgt
    12241 gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat tgtcgccgca
    12301 ggatacgccg acggttatcc gcgccatgcg cctaccggtg ctcctgtttt agtggacggc
    12361 gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga tttaacgcct
    12421 tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat caaaattgat
    12481 gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcact ggcgttacgc
    12541 gtcccggttg tgacagtgta acttgttgta agccggatcg gaggcagcgt cttctggttg
    12601 cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac agagccattg
    12661 cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga actcgccgca
    12721 caggcgttct acataaaacg cttacgcttc attgttgact cggagtcgac ttcagataaa
    12781 tcacgcttca cccttgatgc agattcgggt tcatgttcag attgttgatt aacaggctgc
    12841 gagtcctgtt gtctttccag actcaacagg cccaactgtt gatccaggag ctccaggtcc
    12901 tcctgtacct tcacgcgctc caggagttcc gggtcatcca ggaactccag atccataagg
    12961 tcctccagct cctcgagtcc ttccaggtca acctagcctt ccttgtgcaa aaagtccaaa
    13021 aggtccttca ggccctgctg ttttttcagg ccctcgacgg ctttgagcag cgtcggtaaa
    13081 ttgtctttgt cccacgggtt accattacag tcaaacacct tcaggctctg gggctgcggc
    13141 acctgtaccg ttttaaactt gttttctcca cagtagtaaa ttattagttt gggcggcagc
    13201 cgtggtgaag agatcaaatc gttactagaa cagtcaaata ctttcaggcg agtcaagttc
    13261 tccagcatct catcaggtat tgagttaata ttgttgccgg cacaggatag tttatacagg
    13321 tactggggca tcgacggcgg taaaaattgc aagaagtttt cagaacagtt aatcactctc
    13381 aggttagtcg acagctcagg cagccagata atactgtttt catagcactt cagtaccatc
    13441 agttttgagg gctgcacctt cggtaacaag atcaagacgt tatctgtaca gtcaagcacc
    13501 agcagccact ttggcatcac cttcggtaac gcggtcaaca cgtttttact gcagtaaaat
    13561 tcaatcagat gtggaggcaa attctcaggg agtgaagtca atcccaggtt gctaagattg
    13621 agcatactcc gtcttgtaat gagacattcc ttcattctgc gcaatgcttc tgcccggttt
    13681 tcaatccggc ttgctggcag gttttcttcc aggatatttt ccttgatttc ttccagactt
    13741 tctttcaggt cttcttgggg aatttctgac tgtttgtttt gctggttttc ttccaggatt
    13801 tcgtacaggt ttttttgcag gttttcttgc aaaccatcct gctgtatagc ccattcgcac
    13861 catttggcga ccatcatgaa ctcgttatgt tgctgattca gacgagtccc caacgtgatg
    13921 atggtattgt tgtccacctg tatgttcact ttgcccgcga tactgaggtc aatgatctgc
    13981 cagggggcat caccccagct gaatttttgc gggctgagat acttcatgtt ctgcaaaatt
    14041 tgattcttga gaagagccgc cctggcgggc gtcgtacgcc tgggttaacg gaagatgggg
    14101 gaatggcggg ggcgaatggg cagatgtctt taaagctagc cataatgcta cgtttccttg
    14161 aaataaatat cctctggcag cgccggagac gtgaatgtgg tcaccgcaaa ggagccacag
    14221 gacgtcattt gcgctgatat cactactcat cgttgacttt tttcctggtc agaaatgccg
    14281 tgtcgggtct ggtacatgct gtattacggc ctaggtagct ctgacgtatt aaagaaaata
    14341 cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag gaataactat aagaaatcgc
    14401 tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt gtgggctatg tcagtgcggc
    14461 aggttgcgtg ggggaatatg ttcaggcgat gtcaggcagg ggcccctgat ggttgaccgg
    14521 taatgcgggg ctggttttta ttacctgtta cagtcggaaa aagcccttac gctgactttc
    14581 ttgagccggt gttttgagtt cattagccag agcctgcaat ggtttgtcat ccataattta
    14641 accttcttct gatgttggga tgagcatatc aaaatcagga aatgacaagt ttattacagg
    14701 ctcaggctgc gttcagtaaa gcatcgcatc cggcgataga tgctgcatta aatttaagat
    14761 tcagcttcct cttcagccac ccgaacgcca atcttcaaca cttcattatc ttctttctcg
    14821 gccaccgtcc agatcatccc ggcaaactct acctggtcac cgacaaccgg tgccgcgcct
    14881 agcaactgct ggacaatttc acccagcgtt tgctgtttat cacgatattc tcgcccgtct
    14941 tccaggccat atatcagcgc aacatcagca tatttggcgc tggcttcgag aatgaagtca
    15001 ccaaagaagc gttgatccag cgcgaccggt ggcgactggc tgaacagttt accgagcgcc
    15061 gggagatcgc gttcccgacc aattacacac aatacatcgc cttcgcgcag tcgggtgctg
    15121 ccggtaggat gaagcaactg gttatcacga aacagggctg caatacgcgt ctcttttggc
    15181 atatgcaaat cgcgcagtgc cgcacctaca caccatttat cagcactcaa ttgataaaca
    15241 aactgctccc acggattttc cggatgaata tccaggccaa cgcgtgacac cggacgtccg
    15301 acgggcggaa ccaccacttt ggcttttttc gccgcccacg agagtgatgt tccctgcaag
    15361 agcagtgaaa ccagaaccac aaagaaggcg acattaaaga acagtcgcgc attctccagc
    15421 cccgccatca tcgggaacac ggctaggatg attggcaccg cgccgcgtaa tcccacccag
    15481 ctgataaaca cgcgctcacg cagattgaaa ccgcggaaag ggagcaatcc agcaaatacc
    15541 gaaaggggac gggcgaagaa tatcatccat gccgacaaaa tgagcgccgg aatggcaata
    15601 ggcagcagat cgcttgggtt aaccaatagc cccagcacca ggaacatggc gatttgcgcc
    15661 agccaggcga ggccgtcgaa attttgcagg atgccgtagc ggttgcgaat cggccgatta
    15721 cccagcagaa aaccgcacag ataaacagcc agaataccgc tgccttccag cgccgtggtt
    15781 aaagcaaaaa ttaaaatacc gccgcttaat gccagcaatg gatataatcc ggcaggcagg
    15841 gcgatgcgat taatcatttg cagcagtaaa taaccaccgc caagaccgat gacaatcccg
    15901 aggccaaatt gctgcagaat atcgaccacg aacatccaac tgacactgct ttcatgctgc
    15961 tggatcattg caatcagggt aatcgtcaga aagaccgcca ttggatcatt actgccggat
    16021 tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct taccacccag cagagaaaag
    16081 accgctgcag catcggtgga gccgacgata gcgccgatta ataagccttc aatcaaatca
    16141 agattaaaca gccacgccgc catcatgccg gttaaaccag aggtgataag tacgcccagc
    16201 gtcgccagcg acagcgccgg tcctaacgcc acacgaaagg aactggcctg agtgcgcatc
    16261 ccgccgtcga gcaaaataat cgccagcgcc aggttactta ccatgtaggc gaaggggtaa
    16321 ttatcaaacg ggataccgcc gacgccatcg actcccgcca gcatgccgat cgccagaaaa
    16381 ataaccagaa taggaatgcc aagacgggaa gaaaatgaac taagtaatat actgctggtg
    16441 acaaggatgg atcctaagat gaaaaggcta attattgttg tggcatccaa tggtcggtta
    16501 ctcctgatta cgctgtctct tatataaacc ctaccatatt agcggcagag acagcgtttt
    16561 acttagccct gaagcgtgat ttttttacat tttaagaaca ggatgaccgc taatcgtcaa
    16621 ctgagcgcct tcccgggtgt tattcagaat ggcatgtgcg cccaacggca gcgtaaccgt
    16681 gcgttgttca tgaccaaaat cgaggccggt aatgagtgga atcgacaggc gggaacgcaa
    16741 aaacgcgtac actgactcca ggttgtaacc tgcgtcataa tcattgggcg tgctgccgct
    16801 aaagctaccg agaataatcg ccttctgacg tggcaaaatt cccgcatgat agagctgcaa
    16861 cagcatacgt tcgacgcgga aagggtgcac gttaatatct tccagcacca gaataccgtt
    16921 ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc agcatcgcaa gattgcctcc
    16981 ccacaacgtg ccttcggtct gacatgtcgg gccttcccct tgccattcaa tggtgaaggt
    17041 ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag gcgttcagtt catccgcacc
    17101 aaagtttgct accagcatgg ggccgctaaa ggtgatgaca ttgccttgcg ccagaagacc
    17161 gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagt aacgggtcat gttgttggcg
    17221 agccaccagt gcctgccagt caatatctgc cagtaaacga ctggcaccgt aaccgccgcg
    17281 tacagccagc acgatggtgt tgggggtagt cagtctggca agggaattga gatcttccag
    17341 acgctccgtt tccgtacccg caaaacgctc acaacgacgg gcaataacct cgacgttatt
    17401 gacttgatgc cccgcgtcgg taaggcgctg gataccacgc agcgcggcgt gctgtttaat
    17461 gctgtaaccc gatggggcaa ttaagtgaaa cagagacatg gcaattcctt gctgacaaca
    17521 gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt cacctcaacg gcgattccag
    17581 gctataagga tagaagaagt gaaattgaga tggtttgcct ttttgattgt gttattagcg
    17641 ggttgttcat caaagcatga ctatacgaac ccgccgtgga acgcgaaagt tccggtgcaa
    17701 cgtgcgatgc agtggatgcc aataagccag aaagccggtg cagcctgggg cgtcgatcca
    17761 caattgatca cggcgattat cgctatcgaa tcgggtggta atccgaacgc ggtgagtaaa
    17821 tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct ccggacgtga tgtttatcgc
    17881 cgtatgggct ggagtggtga gccgacgacc agcgagctga aaaatccaga gcgtaatatt
    17941 tcaatggggg cggcttacct gaatattctg gaaaccggcc cgctggcagg cattgaagat
    18001 ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta acggggcagg tgcgctgcta
    18061 cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa tcaacgattt ggatgctgac
    18121 gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc aggctccgcg ctatatctac
    18181 aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg cactttgtcc gctttttccc
    18241 gggcttctcg ctcgagagag aaaataatcc gctgtaattg ccgctccacc gtcgggctga
    18301 cgttaagaaa acggaagctc agacggggag tggtgatggt ttcattcttg ccatcaatca
    18361 ctttgcgctc gctggtggag attaactggg cgtcaaagtg aaaaacaccc cattgcccca
    18421 tgttgacttc aatttgagcg aagcgcatac cttcttgtaa ttcggcaggc tttgctgttt
    18481 ccagtaatgc gcccatgccg cctaacgaca aatcatacaa gcggaaacgt aacgtactgt
    18541 tatccgccag tttggtctgg caaaaataag gcggatggag tggggcggag atgcggaaat
    18601 atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac ggtaataaat gccggaagct
    18661 gcaagtattc actctgctgt agttgttcaa cagtaaactc gactttcgca ccctgagttt
    18721 cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat gttgtcttcg gcttgactgc
    18781 cgaaatccag caccagttta tccggggtta ttgccagtat tttgctgatc agctgcccgc
    18841 cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa atcgcgtaac acgcccagga
    18901 cggctaacgg attttgtttc aggaactgct catggtaatg actcacgcgg aaaaactcct
    18961 gactgacaaa ctgtctttgg tttatcggtc atagttaaca aaacttaata caaatgcgtg
    19021 aatatttttt acatgttgtt cttaaatcag ccgacaaacg cccagcatcg atcccgccct
    19081 tgcctatact tagagcgttg acgtaagcat ttcttgcgac cgattcatcg aaacgagggc
    19141 ctgaacatgg gaattattgc ctggattatt tttggcctga tagccggcat tatcggcaag
    19201 ctaatcatgc cggggcgtga tggtggtgga tttttcctga cctgtattct cgggatagtc
    19261 ggtgcggtgg tcggcggctg gctggcgacc atgtttggca ttgacggctc catcagcggt
    19321 tttaatttgc acagcttcct ggtggcggtg gtgggcgcta tcctggtgct gggcgtattc
    19381 cgcctcctgc gaagagaata agattttcat aaggcggata gcgatacaga tgccgctatc
    19441 cgctttcaca tcagaacgta tactcgacac ctgcccagaa attgcgacct tcttcaataa
    19501 acccttcact gtaggcatat tgggtatcaa acaggttatt gaccgacgca ttgacgctga
    19561 agccatgacc taaggtgtaa tcggctcgaa tgtgggtcac cgcaaaaccg gcggcttttt
    19621 gtgaaccgtc actgttgctg tagctggagg aacgcgcctc ttccgacagc gttacgctta
    19681 acggctccca tggtttgagt gtcatccatg cggtcattgt ctgcgttgga agatcggtta
    19741 tcttgccgat gtctttgcgt ttggcgtcag cgtggatcag agcgtagctc aatcctacat
    19801 ccagtatatt gctgattttc cccttaatac cggcatccag accgctgtaa tccaccgtgc
    19861 cgctgttctg attttgaatg gtatcggcat tgatattgtg cgagaggatg gcatcactca
    19921 cccggttata gtaaacgctg acctcaaatc cccagtcgcg cgtgaaggca ccattccagg
    19981 ttaaatccac cccgcgagcg cgttccggct tgagctgcgg gttaacaatc gctatctggt
    20041 tatacgcagg tttggatgtg gtatagcgtt ctttcagcgt cggaaagcgt gtgcggtcat
    20101 agtacgaaag cgccagagtg tcttcattgg caaagtggta tttccccatc acctgccagt
    20161 taaaagctga ctgattgttg tcgtcataat gggtgatgct gccatctttc tcgtgttttt
    20221 tcgcttctac gctatcgcgc cagtcatagc tgattcccgc cacgacatcg acattatcag
    20281 cagcagccca ttgatattca ctggcgagcg accaggtacg atcttcatag cgatcgtaag
    20341 cggcgtgcgg cgcacctttt tcccggtgta cgtcatcttt ccagttaacg gcaaacgaca
    20401 gcagatcgtt ttcacgcaca tcggctgcca gttgtaatcc ggcaccgtcg ctgtaatcgg
    20461 aataatggct gtagctgcct tttttatttt tcaaatcagc cagcgagttg tacatcatca
    20521 gcgtattttc aaaggtgtcg cgatacagcc gacttttcag ggtaaaacgg tcgtttagtt
    20581 gggtcgctcc ctgataataa aaactttctt tgtcatactc tggccactgc cagtagcgtg
    20641 atttttgacc acaatttccg ctgtatggcg ggttatcttt ttcaccatcc tgcttaatgt
    20701 aagtcagcgt gtattcatcg ttttcgcgcg gtgtaaaacc gagcttcaca atgccgcgtt
    20761 tatcatcagc cgatgaatta atcatcttgc cgtgtttgcc tgcaatatca ttatttacac
    20821 catgcggcag gccgagaaaa tcctgcttta gctggctacc gctgacttgc aaatacccaa
    20881 gatcgctgct ggcggcaaat gaagcatgca tatcgtaggc attgtcctga ctacggctcc
    20941 atccctggcg atatcccaga cttgcttctt cagggtggtg atattaatgg ctccgcccat
    21001 ctgattaggc ccatgaagca gcgacgaata ccctttggaa acttcgactg cccccagatt
    21061 gttggtcaga atccgcgcca gatcgaggtt gccgtcatag gggacataaa tgggcacacc
    21121 gtcgaaatag accggcacct gccgactatc aaagccacga acttttacct gttcttcgtt
    21181 gcggctgcct gacttttgca gcaccacgcc ggggacgaca cttaatgcct gagcgacatt
    21241 ctgtttatcc aaggccttca tggtgggttg atcgagaacg gtggtcgtcg tcgatgatac
    21301 cggactggac catacggtca gcgtgtcgct ctcctgagcc gctacggcct gctgggtaaa
    21361 ggcaagtgat aatgctgtgc agagataacg ttttttgagt ctcatagaat aaattccgtt
    21421 taacaaagaa ttaaatagag gctgtgggtt gcagactgac tttaatgtca gctggtgccg
    21481 cgtaataagg cgcggaggta ataaaaaggc gaatgccgca gtcgaggtaa tttttgagtg
    21541 tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg aggagccagc gacggcgcta
    21601 tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc gagctgaagt acgtctggtt
    21661 gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc ttcaacaaca attttcttct
    21721 cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca gccctgattg tcatgaagaa
    21781 aatggcggtg gttggcaaac aataatatgg tttccgcaca tccggcacga tgaattagtc
    21841 ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt gcccggaatt gcttttcggg
    21901 tgcaggcgat attgccgtca ggataacgtt cacgaagcaa tgccagcatt tgagcgagat
    21961 aatcagaaac accgcaactc cactccagca cattctggac tgccttccat ccctgatgaa
    22021 gtgctgcggc attaccctgc gcacggatta gccgttgacc cgcgttcgct tgtgaaccgt
    22081 cgctgaccgc gtcatcaata gttaatccca gcgtagttaa catcttacac gcgacagaaa
    22141 cgccgctgac gcagccgccc tgacggtgaa aaaacgctat atagccaggc tggtgtccaa
    22201 tatttaatgc tcgcgtggtc aggtcacctc cctggatatc ttccagcagt aacgcatcga
    22261 tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc ggtacgctat cccaccagat
    22321 gagcgcccag tcgcggctgg caggggcgag ggcacgagca tcttgttgtt gataccagtg
    22381 atataaccgc tcacgctcat cgtcgttaag cgcgccaaga ctccagctga cgttctgctc
    22441 aaaacgttcc caggtgctgt tatcctgttg gcaattttgt ccacggataa aatcaacgtg
    22501 ggcataaatt cccatctgat acaggacgtt gagcgcaaag atatagttgg gtaattcgat
    22561 cacttcccga ccagccgccc gctgaatggc tggagagacg aacgaggtgc tgaccagatg
    22621 agttgtatag acgcgaagcc gtgcctgatt attcagtttg ctcattgcat ggcgcatatc
    22681 agccaccaga gtggagcggg aagccacggc aatatcgcag cgtggcaggt cactccagtc
    22741 ctcttcccag gcgcgctgga tccagtggac attgtcagcc ttcagtgcgg cggcgcggcg
    22801 ggcggcaacg ttcagcatcc cctgactgta atcaacgcca taaaccgtag tgagtttgtc
    22861 agccagcgcc agcgatacgg tgcctggtcc gcagcccata tcgaataagg tctgagcgcc
    22921 ttgcaggtca attttagcga tcagttgttg caggtagctg tccgttggcg aggcgcaatt
    22981 ttccgccatt ttctccgccc gttgatccca gtgatcgggc gttttttctg tgcggtgagc
    23041 cagtttgagt tgctgcagat aaaggtctgc aaaatcaata tcatcaatga gcatgattgt
    23101 tccttagcgg ctaattgcag agaggtgatg atgaatttgg tcggcagaaa cccgatacag
    23161 ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag ccttgtgtta ctcgtccatc
    23221 tggctctact tggataatgc tgtcggcaat ggcgttggca tggagcgtat ggtgagtcga
    23281 cattaacatt gtcatgccgt tatttttgag ctgcgccagc gtgtccagaa gttggatctg
    23341 atgaccgaaa tcgaggctgg aggcgggctc atccagtaac aacaagcgcg gttgttggac
    23401 gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg ctaagcgtat tccagcgtcg
    23461 tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc gtggctttca ggcgttcttg
    23521 cttaccggga acggaaaaag cgcctatggt gggggccagg cccatcaaca ccatatccag
    23581 cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc caggcaatag cctgtgcgcg
    23641 ctggcgatgg ctgagtgtct gcacagagag gctatccagt aggatctcgc cttttaataa
    23701 cggtataacg ccaagaatgg agcgcatgag tgtggttttg ccgcaaccat tcgcgccaag
    23761 cagacaacaa attgttcctt gcgacagggt gaaggacacg tcgcgtagca ctggatggcg
    23821 ggagtaaccc agagacgcat gacgaacggc aagcaaagtc actggcgacc tccgcgtaac
    23881 aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca gaatgccaag tgggatttct
    23941 gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga gcagaatagc gccagtacac
    24001 attgccatag gcaacagctg ctgatggtta tgaccggtca acaagcgggc aatgtgcgga
    24061 acgaccagac cgatccaacc aattatgccc gctattgcta ccgtgctggc ggtgattagt
    24121 gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt tcagtcccag cgaacgggct
    24181 tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga gaaaaaacag gggcaaggag
    24241 cctatgagaa tgatcggtgc agcatagcag agatcgcgta aggtcacggt ggacagtccc
    24301 cccagcaatc agaaggtaat tgacggtaac tgggtgtaag gatcggccag cgttttaatt
    24361 agcgatatcc ccgctccgca caacgttcct aacgcaatgc ctaccagcac cagggttaag
    24421 atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga cgcccgcgac caccatcagc
    24481 ccgccgcaaa atgcgtacag ctggatgtaa acaatgggta atccccacag gattgccgcg
    24541 caggcaccaa gtccggcacc ggcggagacg cctaaaatat ccggtgaaac taagggatta
    24601 cgcaacattc cctgataggt ggttccggct cctgccagcg ctgcgcccag taacagcgca
    24661 gcaagaatac gcggcacgcg gatttgccag aagacaattt gttcctgcac agactcattt
    24721 tcttgacgaa gaattagcgc cagcagttgc tgcatatcga gatgataagc cccacttacg
    24781 gcggcaaaag tgatactgat agctgccagc agcaaaatac atccgtttac gattctcatt
    24841 agctcgccac caatttttga tagtcggcat cactgagtga acaatgccag aacagttggg
    24901 cgtaatgctg catgtcactt ttaaactggc gattgatggc gggatccagc caggcgtgaa
    24961 gtctgcgtaa tcccagaaga cggttgattc ccggcggggc atcgagccag ccaaagggca
    25021 agccacttaa aaataagatg cgttgttccg caacggcttt cacgccctgc cagagaggat
    25081 cacgacgaat aaaatctgca gtaacggcct cctgaaccag aataatatcc ggctgccagc
    25141 gcaggagatt ttccatggaa acctgtgtca ggccgtggcg atcggctatt tgcgcgacgt
    25201 tgtgcaaacc taataattcc gccgcctcgg tatgcaacga accctgtaag cccgtttcca
    25261 gccctcgggg accgcgcgca gcataaaagc ggaggttagc ggcgggtgag gtggcgaatg
    25321 cctgagcttc accaacgaag tggctggcga gattggcttg ttctgcggcg cggtgctctt
    25381 cgcctaacgt tttgccaagc gttgttaact gttctgctga ttgcgccagt tttccgttaa
    25441 gcaataacca ggggatttgt gtctgttcgc taacctgccg tgcctgggag atcaaggttt
    25501 catccgtatt gccgcaatca acgaccaaat cgggatgtaa cgccataagc ccttccagcg
    25561 atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg taactggcga atgtgctctg
    25621 gcaatggaat taatgcctga cgggcaaaat caaaagagga aaaaccgacc attttttctg
    25681 gcgcgacagc cagcaagagc aaatcggcgg gcgcgcctgc gctgacgatg cgtgtaatgt
    25741 tttgggcttc gggaagagat tgcgaagcaa aacgattaac tgtttgtgaa aaggacggta
    25801 gagactgcag gagcagggta gatgcaagtg cctgcgcaaa cgtccgtctg gttatgggca
    25861 tggtgaacat cctgaaaagt aaaatcgata tatactttca tatatatcga taaaaatcat
    25921 gttcaatatc aaagtttagc tattgaaaac atgaagatgt gacgacttcc gcaagcctgt
    25981 tgaccctctc tatcattaaa aatgtattta tcaggtggct aatattattt aaatcattta
    26041 aggctaaatt atttaaatat gaatgacatt attgtttttt tatcgtccac ggttacgcga
    26101 cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga aaattatcag
    26161 cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga atgctggggg
    26221 ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt aagcagcgta
    26281 accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt aatgtcatta
    26341 acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg tttattcgga
    26401 cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt tattttgata
    26461 acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag ttgcagttat
    26521 taagcacagg gtgtagcaat gataacattc tcc
//
LOCUS       DAJTNP010000053        25675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTNP010000053 DAJTNP010000000
VERSION     DAJTNP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(20..1384)
                     /gene="yeaG"
                     /locus_tag="OO160_002377"
                     /pseudo
     CDS             complement(20..1384)
                     /gene="yeaG"
                     /locus_tag="OO160_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            1820..2566
                     /gene="mipA"
                     /locus_tag="OO160_002378"
     CDS             1820..2566
                     /gene="mipA"
                     /locus_tag="OO160_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5524469.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2656..3417
                     /locus_tag="OO160_002379"
     CDS             2656..3417
                     /locus_tag="OO160_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5524470.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3468..4352)
                     /locus_tag="OO160_002380"
     CDS             complement(3468..4352)
                     /locus_tag="OO160_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5524471.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4436..5431)
                     /gene="gapA"
                     /locus_tag="OO160_002381"
     CDS             complement(4436..5431)
                     /gene="gapA"
                     /locus_tag="OO160_002381"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5524472.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5773..6186
                     /gene="msrB"
                     /locus_tag="OO160_002382"
     CDS             5773..6186
                     /gene="msrB"
                     /locus_tag="OO160_002382"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5524473.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6228..6500
                     /locus_tag="OO160_002383"
     CDS             6228..6500
                     /locus_tag="OO160_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5524474.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6870..7946
                     /locus_tag="OO160_002384"
     CDS             6870..7946
                     /locus_tag="OO160_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5524475.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7973..9352
                     /locus_tag="OO160_002385"
     CDS             7973..9352
                     /locus_tag="OO160_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524476.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9369..10414
                     /locus_tag="OO160_002386"
                     /pseudo
     CDS             9369..10414
                     /locus_tag="OO160_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10435..11271
                     /locus_tag="OO160_002387"
     CDS             10435..11271
                     /locus_tag="OO160_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5524477.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11276..12223
                     /locus_tag="OO160_002388"
     CDS             11276..12223
                     /locus_tag="OO160_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5524478.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12233..13213
                     /gene="ydjG"
                     /locus_tag="OO160_002389"
     CDS             12233..13213
                     /gene="ydjG"
                     /locus_tag="OO160_002389"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5524479.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13350..14108
                     /locus_tag="OO160_002390"
     CDS             13350..14108
                     /locus_tag="OO160_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5524480.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14226..15584
                     /locus_tag="OO160_002391"
     CDS             14226..15584
                     /locus_tag="OO160_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524481.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15677..16318)
                     /gene="pncA"
                     /locus_tag="OO160_002392"
     CDS             complement(15677..16318)
                     /gene="pncA"
                     /locus_tag="OO160_002392"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5524482.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16329..17345)
                     /gene="ansA"
                     /locus_tag="OO160_002393"
     CDS             complement(16329..17345)
                     /gene="ansA"
                     /locus_tag="OO160_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5524483.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17512..19368)
                     /gene="sppA"
                     /locus_tag="OO160_002394"
     CDS             complement(17512..19368)
                     /gene="sppA"
                     /locus_tag="OO160_002394"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5524484.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19529..20080
                     /locus_tag="OO160_002395"
     CDS             19529..20080
                     /locus_tag="OO160_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5524485.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20197..21240
                     /gene="selD"
                     /locus_tag="OO160_002396"
     CDS             20197..21240
                     /gene="selD"
                     /locus_tag="OO160_002396"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5524486.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21245..23206
                     /gene="topB"
                     /locus_tag="OO160_002397"
     CDS             21245..23206
                     /gene="topB"
                     /locus_tag="OO160_002397"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5524487.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23333..24373
                     /locus_tag="OO160_002398"
     CDS             23333..24373
                     /locus_tag="OO160_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5524488.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24490..>25675)
                     /gene="gdhA"
                     /locus_tag="OO160_002399"
     CDS             complement(24490..>25675)
                     /gene="gdhA"
                     /locus_tag="OO160_002399"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5524489.1"
                     /translation="LVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRF
                     HPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELY
                     RHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGY
                     GLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDE
                     SGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDV
                     DAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQN
                     AARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQG
                     VI"
BASE COUNT         6520 a         5977 c         6626 g         6552 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaatctgct gttccaggac gtagaacaga tggaccgggt
       61 ttgccgccac ttctacatga tcgaagttga acacgcggga gaggatctta aacgcaaaac
      121 gcgtcgacag accgttcatc ccttcatcga caccggcgta gtcacgatat tcctgatacg
      181 acttggcttt cggatcggtg tctttcagac tttcgccatc ataaacccgc atctttgaat
      241 aaatgctgga gttttctggc tctttcaggc gcgaaagaat ggaaaaacgt gacagtgttt
      301 cgagcgtgcc aggggcgcat ggggcgtgag tcaattcact gtgattaagc aatttctcgt
      361 agattttgat ctcttcggaa atgcgcaagc aatacggcac cttcacgatg taaacacggt
      421 cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac ccattcggac ttgttcgagt
      481 gggcgagaat aatcccgttg aacggcaggg cggagatacc ttccgtcccg ttgtagttac
      541 cttcctgagt ggcggttaac aagggatgca gcacttttat cggtgcttta aacatctcaa
      601 cgaattccat gatcccctga ttggcgcggc acagcgcacc ggaatagccg taggcgtccg
      661 ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac tttcccaacc agcgcggaga
      721 tgtcctggtt gttctcatca ccgggttccg ttttggcgat agcaatttgt tgcagaattg
      781 acggccagac cttcactacc cggaacttag tgatatcgcc accaaattca tgcaggcgtt
      841 ttgccgccca tggcgacatg atggtgccga gataacggcg aggaatgcca tactcttttt
      901 ccagaatctg agcatcttcc tgcggattga aaagacagaa cggatgatcg ttgaccgggc
      961 tacgctcacc gttggcgctc aatacataaa tcggtacgag ctgcattaat gatttcagtc
     1021 gctcagcaag cgatgattta cccccaccca caggccccag cagatacagg atttgtttct
     1081 tctcttccag cccctgagcc gcgtgtttca gataagagac aatctgttca atcgcgtctt
     1141 ccatgccgta aaactcttca aatgccggat aacgtgcaat gacccggtta gaaaagagtc
     1201 gagaaagtct ggggtcctgg gctgtatcga ccatgacagg ctcaccgata gccatcaata
     1261 gccgctcagc cgcgttggca taagcactgc gatcttgccg acaagtggta agaaactcct
     1321 gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg gcgatagtga tcgaatatat
     1381 tcatggtatg ccgtcctttc gttttttagc acaggttaag agccgttcat atgaatagta
     1441 gaggctcccg gaagaggtaa actgaacgac gtctcttgct aaaacacagc aactctcatg
     1501 ccattttatc gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga
     1561 tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca
     1621 tccataaatt ttgcataatt aatgtaaaga ccaggctcgc cagtaacgct aaatccattt
     1681 ggctgtaagc gcggtgtcat ccgcggcagg aaaattaaac agttacttta aaaaatgaaa
     1741 acgtaaaaag gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt
     1801 gtttaaggaa tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc
     1861 aacgtctgca ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtaggtgt
     1921 cgttgagcac ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta
     1981 tgaaggcgat aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc
     2041 aacggataaa ctttcaatta ccgcttactg gtcgccgctt tacttcaaag cgaaagacag
     2101 tggcgatcac caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc
     2161 ttatgctcac tttacccagt acggttacct gcgtaccacc ctggctggcg ataccctgga
     2221 taacagcaac ggcattgtct gggatatggc ctggttgtat cgttacacca acggtggcct
     2281 gaccgtgact ccgggtattg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta
     2341 tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaatgacag
     2401 ctggagccct taccttgagc tgagcgccag ctacaacttc ctcggcgact ggagtgttta
     2461 cggtaccgcg cgctacaccc gtctgtctga tgaagttact gacagcccga tggtggataa
     2521 atcctggact ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatctttcc
     2581 agggcatttt gtgcataaaa acagggcgtc acgccctgtt ttgcattacg gtggtgcaat
     2641 acctgggagg aaacaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca
     2701 gccatagggc agggaacatg gtatatgggc gaagatgcca gtcagcgcaa aacagaagtt
     2761 gctgcactac gcgcaggcat tgaactcggt ttaaccctca ttgataccgc cgaaatgtat
     2821 gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa ccggtctgcg agagaaggtc
     2881 tttctcgtct ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc
     2941 gaagccagtt tacgccgtct caatactgac tatctcgatc tctacttatt acactggtct
     3001 ggcagtttcg cttttgaaga gactgttgct gcgatggaaa aattgatcgc ccagggaaaa
     3061 atccgtcgct ggggcgtttc taaccttggt tatgccgata tgcaggaact ctggcagctg
     3121 ccggggggaa atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt
     3181 gagtacgatc tactcccctg gtgccagcaa cagcagatgc cggtgatggc ttacagtcca
     3241 ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa acgcggtagt caacgaaatt
     3301 gcacatgtcc aacaacatgc agctgcgctt gaggtcgaac tttcgtcagc ggaattagct
     3361 atgctggata aggcatatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt
     3421 tgatacgtgc cggatgcatc gcacccggca cacaacatta acgcaaatta acgtttggca
     3481 acgcgaatgg attgcgccag atgtgcaggt ttctctttgg tgactttttg cgtttctgaa
     3541 gcgtaagccg tttctacaca aacaaaggtc ttgtagccat cgtccggcat gtcgcccatg
     3601 ctaactgaaa gcgccggtcc cgggttccag ccgacaacgt tcagatgatg ctggtggcct
     3661 acggcgataa tacgattcag cgcttcatca ttaatcacgc tgcaatcttg cggattcaga
     3721 tacacgcgat cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc
     3781 gcgtcattca ctttatcaat gaagcgatcg cccagcccac tgacgcttac cttagcgata
     3841 tcacccacgt taaagtaggt atgcagggca gaggtggttt caaattcgcc atgtgattca
     3901 agatcgattt cacaggtttt acccacgcgg aaatgcgcta acagcgtaaa gtcgtgcggc
     3961 cagaattttt tcgtctcttc gctttgtgtc aattcaaaag tcagcgctac gccatcagca
     4021 tcttcatgat gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga
     4081 ccttgttgtg ccgccggacc aaaccacggc cagcaaaccg gtacgccacc gcgaatagcg
     4141 acgccatttt tgaacggtgt gttgttgctc aaccacagaa cttcttcttc acccgcaggt
     4201 ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg cttttacctg gggatgatcg
     4261 accacaatga ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg
     4321 atgaccggaa gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca
     4381 ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca cagtgtcatc tcaacttatt
     4441 tggagatgtg agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc
     4501 aggataccag tttcacgaag ttgtcgttca gagcgatacc agctttagca tcgaacacgg
     4561 aagtgcaaac ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa
     4621 cgcctttcat ttcgccttca gcagcagctt taacggcagc tttgatctgc tcgtaagttg
     4681 cagctttttc cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga
     4741 acgccatacc agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag
     4801 caccggtaga ggacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt
     4861 gagacgggcc atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac
     4921 cttcgatgat gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg
     4981 tgcaggaagc gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa
     5041 cgaacatcgg agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg
     5101 tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt
     5161 caacacccac ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt
     5221 ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt
     5281 gagtggagtc atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg
     5341 caacgatctc gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc
     5401 caaaaccgtt gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata
     5461 aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca
     5521 ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg actcgcctca cttttccttt
     5581 gggcttgaga ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg
     5641 tgattttcat cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt
     5701 tgccagttgg tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg
     5761 agatgtgagt aaatggcgaa taaaccttcg gcagaagaac tgaaaaaaaa tttgtccgag
     5821 atgcagtttt acgtgacgca gaatcatggg acagaaccgc catttacggg tcgtttactg
     5881 cataacaagc gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc
     5941 gaaaccaagt atgattccgg ctgtggctgg cccagtttct acgaaccggt gagtgaagaa
     6001 tccattcgtt atatcaaaga cttgtcacat ggaatgcagc gcatagaaat tcgttgcggt
     6061 aactgtgatg cccatctggg gcatgtcttt cccgacgggc cgcaaccaac aggcgaacgt
     6121 tattgtgtta actctgcttc tttacgcttc actgatggcg aaaacggcga agaaatcaac
     6181 ggttgaaaaa acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg
     6241 acattatcaa cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg
     6301 ggaaatggcc tgatggcgtt gcgttgaccg aggaacaaaa agagaattgc ctgcaactgg
     6361 tgatgctgtg gcaagcccgc cataataccg aagcacagca tatgacaatt gacacaaatg
     6421 gtcagatggt catgaagagc aaacagcagt taaaagaaga ttttggtatc tcagcaaagc
     6481 ctattgccat gtttaagtaa gtggttcatc ccctttttgc cgcggctatg aaggggatag
     6541 ccaacaatga ccccacgact cgtctcaatt atctcgaccc gacctggcta agtcccagtc
     6601 cgatctcttg cagtcgcgtt cacgcctgaa tgacttctcc tgaacttaag tctcagacct
     6661 atttggccgg taatccctct cgaatttgct ctttttgcgt cccgccttcc accaacctgt
     6721 aacagaccca gccattttct gcgtaattgt gatttgagtt gtaaaatgca ggtcattatc
     6781 tgtgttgtgc agatttaata ttgacctcaa aaatcatgtg ggtaagtatg atggccatct
     6841 aagcaaaaca aaacacagag gatgacgcaa tgaaagcact ggctcggttt ggcaaggcct
     6901 ttggcggcta caagatgatt catgtcccac aacccatttg tggcccggaa gacgtagtga
     6961 ctgaaattaa agccgcggca atctgcggcg cagacatgaa gcactacaat gtcgatagcg
     7021 gttctgatga gtttaactct atccgcggcc atgagttcgc aggttgtatt gcgcaggttg
     7081 gtgaaaaagt caaagactgg aaagtggggc aacgcgtcgt gtcggataac agcggtcacg
     7141 tttgcggcgt ttgtccggcc tgtgaacagg gtgattttct gtgttgtaca gaaaaagtaa
     7201 accttggtct ggataacaat acctggggcg gtggtttttc caaatattgt ctggttcctg
     7261 gtgaaattct caaaattcat cgtcatgcgt tgtgggaaat ccctgatggt gttgattatg
     7321 aggacgcagc cgtacttgac cctatctgta atgcctataa atccatcgcg cagcaatcga
     7381 aattccttcc tggtcaggat gttgtcgtca ttggcactgg cccactcggg ctgttttccg
     7441 tacaaatggc gcggattatg ggggcggtaa atatcgtcgt cgttggtctg caagaagatg
     7501 tggcggtccg cttcccggtt gcaaaagaac tgggggcgac ggcagtagta aatggttcta
     7561 ccgaagatgt ggtggcgcgc tgccagcaaa tttgtggcaa agataatctg ggactggtga
     7621 ttgaatgctc cggtgccaat atcgcactga aacaagccat cgaaatgctc cgcccgaatg
     7681 gggaagtggt acgcgttgga atgggcttca aacctcttga tttctcgatt aatgacatta
     7741 ccgcctggaa taaaagcatc attgggcata tggcctatga ctccacctca tggcgtaacg
     7801 ctatccggct attagccagc ggcgctatca aagtcaaacc gatgatcacg catcgtatcg
     7861 gcctgtcgca atggcgcgaa gggtttgatg cgatggtcga taaaaccgca atcaaagtga
     7921 tcatgactta cgactttgat gaataactac tctcctgaag gaacgttatc gcatggaaca
     7981 gataacaaaa ccgcattgtg gtgcacggct ggatcgctta ccggattgcc gctggcattc
     8041 gtcaatgttt gccatcgtcg cgtttggctt gcttgtctgc tggagtaatg ccgttggtgg
     8101 cttgatcctc gcgcagctga aagcgttggg ctggacagat aattccacta cagccacatt
     8161 ctcagcaatc acgaccgccg gaatgtttct cggtgcgctg gtgggcggca tcattggcga
     8221 caaaaccggt cgcagaaatg cgttcatcct ctatgaggcc attcatattg cctcgatggt
     8281 tgtcggtgct ttctcaccga atatggattt tcttattgcc tgccgttttg tgatggacgt
     8341 tgggctggga gctttactgg ttacactgtt tgctggtttc accgaatata tgcccggtag
     8401 aaatcgcgga acgtggtcca gtcgggtttc ctttattggc aactggtcat atccgctctg
     8461 ctcactgatt gcgatgggac tcacgccgct gattagtgca gagtggaact ggcgggtaca
     8521 actgcttatt cctgcaatat tgtcgcttat cgctacggcg ctggcctggc gctactttcc
     8581 tgaatccccg cgctggctgg aatcgcgcgg acggtatcag gaagccgaga aagtcatgcg
     8641 gagtatagaa gaaggcgtca tacgccagac gggtaaacct ttgccgcctg tggttattgc
     8701 tgatgacggt aaagcgccac aagcggtgcc gtattcagcc ttactgacag gagtattact
     8761 gaaacgcgtg atattaggct cttgtgtgct gattaccatg aacgttgtgc agtacacact
     8821 aattaactgg ttgccaacaa tattcatgac ccaggggatt aatttaaaag actcgattgt
     8881 tttaaatacc atgagtatgt ttggcgcgcc atttggtatt tttattgcca tgctggtgat
     8941 ggataaaatt ccgcgtaaaa caatgggtgt ggggctatta atcctgattg cggtgctcgg
     9001 atatatctat tcactgcaaa ccagtatgtt gctcataacg ctgattggtt tcttcctgat
     9061 tactttcgtc tatatgtacg tttgctatgc ctcggcagtg tatgtccctg aaatctggcc
     9121 gacagaggcc aaactccgtg gctccggtct ggcgaatgcg gtagggcgaa tcagtggtat
     9181 tgccgcacct tatgccgttg cagtgctgct cagtagttat ggtgtaacgg gagtctttat
     9241 tcttctgggg gctgtttcaa atattgtcgc aattgctatc gccaccattg gaattgaaac
     9301 caaaggtgtc tccgttgaaa gtttaagtat tgatgcagta gccaataaat aatacgggtg
     9361 gattcaaaat gaaaaattca aaagcaatat tgcaggtgcc gggcacaatg aaaattattt
     9421 cagcagaaat accagtgcct aaagaagatg aagttttgat taaagtagaa tatgtcggta
     9481 tttgtggttc agatgtacat ggttttgaat caggcccgtt tattccgcct aaagacccaa
     9541 atcaagaaat tggcctgggt catgaatgcg ccgggacggt tgtggctgtg ggaagccgcg
     9601 tgcgcaaatt taaaccgggg gatcgggtaa atatcgaacc tggcgttcct tgcggtcact
     9661 gtcgttactg tctggaaggc aaatataaca tctgcccgga cgttgatttt atggcgacac
     9721 aacccaacta ccgcggcgca ttaacgcact atctgtgtca tccggagagc tttacttaca
     9781 aactgcccga caatatggac acgatggaag gggcgctggt ggagcctgcc gcagtcggga
     9841 tgcatgccgc gatgctggca gatgttaaac cgggtaagaa gataattatt ctgggagcag
     9901 gttgtattgg tttgatgacg ttgcaagcgt gcaaatgcct gggcgcaacg gaaattgccg
     9961 tcgttgatgt actggaaaaa cgtctggcaa taggggaaca gcctggtgcg acagtggtta
    10021 ttaatggcgc aaaagaggac actattgcac gctgtcagca atttaccgaa gacatgggcg
    10081 cagatattgt tttcgaaaca gcgggttctg cggtcaccgt taaacaggca cctttaatct
    10141 ggtaatgcgc ggcggtaaaa ttatgattgt tggtactgta cccggcgatt cggcaatcaa
    10201 tttcctcaaa atcaatcgcg aagtcactat ccagacggta ttccgctatg ccaatcgtta
    10261 tccggtcacg attgaagcta tttcttcagg gcgattcgat gtgaaatcga tggtgacgca
    10321 tatttacgat tatcgggatg tacaacaggc atttgaagag tcagttaaca acaaacgcga
    10381 cattattaaa ggcgttatta aaattagcga ttaattaata cgggagaaca gaacatgctc
    10441 gcagatatca gatattggga aaacgatgcc acaaataaat attatgcaat tgcccatttt
    10501 aacgtatgga atgcagaaat gttgatgggc gttaaagacg cagccgaaga agcgaaatcc
    10561 ccggttatta tttcttttag tacaggtttt gtcggtaaca cctcatttga agatttctct
    10621 cacatgatgg tatcaatggc acaaaaagca acggtgccgg taataactca ttgggatcat
    10681 ggtcggagta ttgagattat tcataacgcc tggactcatg gtatgaattc attaatgcgt
    10741 gatgcttctg catttgattt cgaagaaaat attcgtttaa ccaaagaggc tgttgatttc
    10801 tttcatccgc tgggtattcc ggtagaggcg gaattagggc atgtcggtaa tgaaaccgtt
    10861 tatgaggaag cgctggcggg ctatcactat accgatcctg accaggcggc tgaatttgtt
    10921 gaacgtacgg gctgtgactc attggctgtc gccatcggca accagcatgg ggtttatacg
    10981 tcagagccgc aattgaactt tgaggtcgtc aaacgcgtac gcgatgctgt ttctgttccg
    11041 ctggttttgc atggcgcatc ggggatcagt gatgccgaca ttaaaactgc aatttcgttg
    11101 ggtattgcga aaatcaacat tcatacggag ctctgtcagg ccgcaatggt agcagtgaaa
    11161 gaaaaccagg atcagccttt cctgcattta gaacgtgaag tacgtaaagc tgtaaaagaa
    11221 cgggcattgg aaaaaattga actgttcggt tctgatggca aagcggaata atgatatgga
    11281 taatctcgac gttatttgta taggtgccgc tattgttgat attccattac aaccggtcag
    11341 taaaaatatc tttgatgtag attcttatcc tcttgaaaga atcgcaatga gcaccggggg
    11401 cgatgcaata aatgaagcaa caattatttc tcgtctgggg catcgtaccg cattaatgag
    11461 ccgcattggt aaagatgcct cagggcaatt tattctcgac cattgtcgca aagagaatat
    11521 tgatattcaa agcctgaaac aggacgtcag catagataca tctattaacg ttggactggt
    11581 gacggaggat ggcgagcgga cgtttgtcac gaaccgtaac ggcagtttgt ggaaattaaa
    11641 tattgacgat gttgattttg cgcggtttcc tcaggcgaaa ttattatcac tggccagtat
    11701 tttcaacagt cctctattgg atggtaaagc gctaacagaa atttttacgc aagcgaaagc
    11761 ccggcagatg atcatttgtg ccgatatgat caaaccgcgg ttgaatgaaa cgctggatga
    11821 tatttgcgaa gcattgagtt atgtcgatta tctgtttcca aattttgccg aggaaaaatt
    11881 actcacaggg aaagagacac tggatgaaat tgctgactgc tttcttgcgt gcggcgtaaa
    11941 aacggtggtg attaaaacgg gtaaagacgg ctgctttatc aagcgtggtg acatgacgat
    12001 gaaggtgccg gcggtcgcag gaataaccgc catcgacacc attggcgcgg gcgataactt
    12061 tgcttcaggt tttattgcgg cactgttaga aggcaaaaat ctgcgtgaat gcgcacgctt
    12121 tgccaatgca acggcggcta tctcggttct aagcgtcggt gccaccaccg gcgtaaaaaa
    12181 cagaaagctg gtcgaacaat tgctggaaga atacgaagga taatgaaggc aaatgaaaaa
    12241 gataccttta ggcacgacgg atataacgct ttcgcgaatg gggttgggga catgggccat
    12301 tggcggcggt cctgcatgga atggcgatct cgatcggcaa atatgtattg atacgattct
    12361 tgaagcccat cgttgtggca ttaatctgat tgatactgcg ctaggatata actttggcaa
    12421 tagtgaagtt atcgtcggtc aggcgttaaa aaaactgccc cgtgaacagg ttgtagtaga
    12481 aaccaaatgc ggcattgtct gggaacgaaa aggaagttta ttcaacaaag ttggcgatcg
    12541 gcagttgtat aaaaaccttt ccccggaatc tatccgcgaa gaggtagaag ccagcttgca
    12601 acgtctgggt attgattaca tcgatatcta tatgacgcac tggcagtcgg tgccgccatt
    12661 ttttacgccg atagctgaaa ctgtcgcagt gcttaatgag ttaaaggctg aagggaaaat
    12721 tcgcgctata ggcgctgcta acgtcgatgc tgaccatatc cgcgagtatc tgcaatatgg
    12781 tgaactggat attattcagg cgaaatacag tattctcgac cgggcaatgg aaaacgaact
    12841 gctgccgcta tgtcgtgata atggcattgt ggttcaggtt tattccccgc tagagcaggg
    12901 attgttgacc ggcactatca ctcgtgatta cgttccgggc ggcgctcggg caaataaagt
    12961 ctggttccag cgtgaaaaca tgctgaaagt gattgatatg cttgaacagt ggcagccact
    13021 ttgtgctcgt tatcagtgca caattcccac tctggcactg gcgtggatat taaaacagag
    13081 tgatttaatc tccattctta gtggggctac tgcaccggaa caggtacgcg aaaatgtcgc
    13141 ggcactgaat atcaacttat cggatgcaga cgcaacattg atgagggaaa tggcagaggc
    13201 cctggagcgt taaatactca acacgattca cctctgtttc tgtgaaaact gtgatgaaaa
    13261 tctgtgattc aaataggtta ttttgaaagt aaacatcggt tatgcgataa tcgcgctaat
    13321 gtgatgtaaa aagatacagg ggtgtaatcg tggcggcaaa agacaggatt caggcaatta
    13381 agcaaatggt tgccaacgat aaaaaggtga cagtctcaaa tttgagtggg atttttcagg
    13441 taaccgaaga aaccattcgc cgcgatcttg agaagctgga agatgaaggc tttttgacca
    13501 gaacctatgg tggtgctgtt ttaaatacag cggtgttgac ggagaatatc catttttata
    13561 agcgcgcttc atcgttttat gaagagaagc tgcttattgc acgcaaggca ctacccttta
    13621 tcgacaataa aaccaccatg gcagccgatt ccagtagtac cgtaatggaa ttgctcaaat
    13681 tgttacagga ccgtagtgac ctgacgttgc taaccaactc ggcggaagcg attcatgttc
    13741 tggctcagtc agaaattaaa gtcgtttcaa cgggtgggga actaaacaaa aatacacttt
    13801 ccctgcaagg aagaattact aaagagatca tcagccgcta ccatgtcgat atcatggtaa
    13861 tgagttgtaa aggtcttgat attaacagcg gcgcgctgga ctctaatgaa gcggaagctg
    13921 aaatcaaaaa gacaatgatc cgtcaggcga cagaagttgc gttattggtt gatcactcta
    13981 agtttgatcg taaagctttt gtccagttag ctgactttag tcatattaat tatataataa
    14041 ctgataaatc accgggtgca gagtggatag cattttgcaa agacaataat atccagctgg
    14101 tgtggtaatt taagcacacg ttgtcaatat tatttgttcc tgcgactgga aataaaatga
    14161 tgataaagat gcggcaagtg aatttgccgc aattgctacc ttttatagca aatccgaggc
    14221 acccaatgga acaatatgat caaattggcg caagactgga ccgcttgcct ttggcccggt
    14281 ttcattatcg tatatttggt ataataagct ttagtctgtt attaacgggg tttttgagtt
    14341 actcaggtaa tgtcgtctta gcaaagctgg taagcaatgg atggtcaaat aatttcctct
    14401 atgccgcctt tacctcggca ttaatgtttg gttatttcat cggctcactt actggtgggt
    14461 ttattggtga ctactttggg cggcgcaggg cgtttcgcat aaatcttctc atcgtcggta
    14521 ttgctgcaac aggggccgct tttgtccctg atatgtactg gctcattttc tttcgcttcc
    14581 tgatgggaac aggaatgggg gcgctgatta tggttggcta tgcctcattt acggagttta
    14641 tccccgcgac ggtgcgtgga aaatggtccg cgcggctctc atttgttggt aactggtcgc
    14701 ccatgctgtc tgcggcgata ggcgtggtgg ttatcgcttt ttttagttgg cgaataatgt
    14761 ttcttctggg tggtattggc atactgttag cctggtttct ctcaggtaaa tactttatcg
    14821 agtcgccacg atggctggaa gggaaagggc aaatcgccgg tgcagaaagc caacttcgtg
    14881 aagtagagca gcaaattgaa agggagaaga gtattcgttt accccagctt actttgaacc
    14941 agagcaacag caaggttaag gtaatcaagg gtaccttcag gctcctgttt aaaggggaaa
    15001 tgttacgacg tacattagtt gcgataactg ttttaattgc aatgaacatt tcgctttata
    15061 ccattaccgt atggataccg accatatttg ttaactccgg cattgatgtc gataaatcaa
    15121 tattaatgac cgctgttatt atgattggtg ctccggtagg aatatttatt gcggcattaa
    15181 ttattgatca ttttcctcgt cgattatttg gctccgcctt acttattatt attgccgtgt
    15241 taggctatat ctattcaagt cagactacag agtgggcgat tttaatctat ggtctggtga
    15301 tgatcttctt tttatacatg tatgtttgct tcgcgtcggc ggtttatatc ccggagcttt
    15361 ggccaacgca tttacgcctg cgcggttcgg gtttcgttaa tgccgtcgga cggatcgtcg
    15421 cagttttcac gccctatggc gttgcggcat tattaacaca ttatgggtcg atcacggtgt
    15481 ttatggtgct tggtgtcatg ttagtgctct gtgcgctggt tctctccatt tttggcatcg
    15541 aaacgcggaa ggtgtcgctg gaagagattt ctgaggtgaa ttaaatcttt cgttgatggc
    15601 atcaattgcc agatgtacca ctgacgtgtt ttatttggcc tacaatgcca gtacagaatt
    15661 gtaggccagc gtaaaattac ccctgcgtct cttcccagtc tgccagcgta tatagcgttg
    15721 ccccagctgc tgacatctcc ataaacgcgt gtgcactgtc ctggggctgg atattcacgc
    15781 cacgacagcc atcggtaatc acgtttacct tataacctaa ctgtaacgcg tccagcacgg
    15841 taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg atttcatgat
    15901 cgcgcaacca gtcatcaagc gcggttttct gccgacgtcc gttatcaaaa aaggcactgt
    15961 aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatctct ttttgattca
    16021 gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgttcaggc cagaaagttt
    16081 gccgcaggcc gccgagttgg cctggcgtat aaggctctac gccgtgctgg ctggcaaagc
    16141 tgccgtgatt cgccggatgc cagtcctggc tggcgataac cgcttctccg cgcgactggc
    16201 accagtcaat aaggcggtta gcaacctcca ccgtactgtc accttccggt acggcgagcg
    16261 cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga tggggcattg
    16321 cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt tttggctcat ggccttgcga
    16381 atggtttcag tatccagttc ctggctcagc aggtaatgca gtttggttag cgtggcttct
    16441 acagtcatat ctgcaccgtc aataacgccg gcatgggcga gggcgttacc ggtggcataa
    16501 ccactcatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac aataccgcga
    16561 tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt acccacgcca
    16621 taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac gacgtcagca
    16681 gaaatccctg gataaatcgt caccacgcca attggttgtg gcgtgattgg atgaacgatt
    16741 aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg gataccagct
    16801 tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc atgggctttg
    16861 gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc gttgatcgga
    16921 taattcggcg caacgtacaa cgcattcagc agattaattt gtccgtcaga gcgtaactca
    16981 gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattctc gagcatgaac
    17041 gacagcgcag aggcggtata cgccatcgtg tcggtaccgt gtagaatgac aaaaccatca
    17101 tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc cggcgtcata
    17161 tcagaagaat ccatcaacgg cgtatattca tgaatggtga aatctggcat ctccgggcga
    17221 tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg aatataaccc
    17281 tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt
    17341 tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga ggcaggagga
    17401 gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa gaggattcgt
    17461 cgaggtaaaa aataatgtgg ccgacgatct gtcggccact cagtacaaga cttaacgcac
    17521 gttggcgcag gtcaggcaaa acgcataacg gttttgtggg tcgttaaacg cggccagctt
    17581 gtcgctttca cttttaacgg tagaggctac cgaggcaagc ggtgcaggta acatggcctg
    17641 gaacgcatct ggcaacattg cccggacaga accagacatg ttgtccatca ctttgtcgaa
    17701 gaaggtcggt tcatcaacgt agtattccag atgccactgt ttcacttttg cgagctctgc
    17761 tgctttggca accgcatcat cgaaatcccc gagactatcg accagcccgt tagcttttgc
    17821 atcctgaccg gtccagacgt ggccctgggc gattttatca atttgctccg gcgtcgaatg
    17881 acgcgcatca gcaaccagcg tgataaagcg tttatagcca ttctcaatgc ttaattgcat
    17941 catctgctgc gcttccggcg gcagtgccct ggtgatagaa acatccgcca gcggtgaagt
    18001 tgagacacca tcagtatgaa caccaatcga atccagacta ttttctacgg tggtgatcac
    18061 gccgaagata ccgatagaac cggtcagggt gctggggtta gccacaatgt aattagctgg
    18121 cgtggaaatc cagtaaccac cagatgccgc catgccgccc atcgatacaa ccacagactt
    18181 acccgctgcc cgggctgctg ccagttcagc gcgaatcact tcagacgcgg taacgctgcc
    18241 gcctgggcta ttaacacgca ggacaatcgc tttcactttc gggtcaaggc gagcgtcgcg
    18301 gatttgtgcc gcagtggtat caccgccaac attcccctga gtttcctcgc catccataat
    18361 tgcgccatta gcgaagacga caccgatgct gtcaccggta tctgccggcg ttttcaatgc
    18421 gtaatcgtaa taactgatgg cgcgataatt tttatcagtc ttactccagc cgaactcttt
    18481 ggtcagagct ttttcgattt cggcactcga tgccagtgca tcgaccagct tgttttccag
    18541 tgcatatttc gcggtatcgc caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc
    18601 agggaatacc tgctgagcag ggatctgccg gttagcggca acagtattca gatagttttg
    18661 ccacagctca ccaatccagc ggctgtcagc ttcgcgggct gccggtgaca tatcatcacg
    18721 aataaacggt tcaacggcag atttatacgt acccacgcgg aacacatggg tggaaacttt
    18781 cagcttatcc agcaacgatt tgtagtacag accgttggta gcaaaaccgt gcagatcaac
    18841 cacgccttgc ggtgacagcc aaattttatt ggcgaaactg gcgagataat attgcccctg
    18901 gctgtagttc tcgccaacgg cataaaccgg tttcccgctg tcacgaaact ctttcagggc
    18961 tttgccgatg tactgcatag acggctggtc gccgcctgcg aagtttttca gatccatcac
    19021 gataccggtg atattgcggt cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag
    19081 tgagttttcc tgcagacgat cggaactggc accaagcagc tggcggctta atttactaaa
    19141 ccgctgagaa ctgtctggtt tatcgacgat cacaccagaa atgtccagca gcagtgcgcc
    19201 acgactggcc gtttctttcg aatcaccacc actgacctgc atccagatcc ccacaccaac
    19261 cagtacgagg aaaataaaga acaggttaag taccatttca cggacgaaat tcagcagacg
    19321 ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg gttcgcatgt attctcccaa
    19381 cttaaggtca cacctgtcac aggcgcaata gatgtggtta tcgtaatgac ccaaccggca
    19441 cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc actctcgtgt taattttgtg
    19501 aataaatatc acgacaggag ttaatcaaat ggatgcactc gaactattga tcaatcgccg
    19561 tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg
    19621 tgcgggtatg cgtgcgccgg accataagtc catgcaaccg tggcattttt ttgtgattga
    19681 aggggaaggg tgcgagcgtt tcagcgccgt actggaacag ggggcgattg ctgccggtag
    19741 tgatgataaa gctatcgaca aagcccgtaa tgcgccgttc cgcgcaccgc tcatcatcac
    19801 ggtggtggcg aaatgcaaag aaaatcataa agtcccgcga tgggaacagg aaatgtctgc
    19861 cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc cagggatttg gcggcatctg
    19921 gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca
    19981 ggataaaatt gtcggttttc tctacctcgg cacgccgcag ttgaaagcat ctacgtcgat
    20041 taacgtcccg gacccgacgc cgtttgtaac ttatttctga taattgtcgc gaaactgtct
    20101 ggattgtgag caaaggcgca ggaattcaga cactctcact tatcacttca cggaatgagg
    20161 gttaccatac ccggattgca ttgacaggag atgtccatga gcgagaactc gattcgtttg
    20221 acccaataca gccacggagc tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc
    20281 atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt tgcttgtggg taatgaaacc
    20341 cgcgacgatg cggcggtgta cgatctgggc aatggcacca gcgttatcag taccaccgac
    20401 ttctttatgc cgatcgttga taatcctttc gattttggcc gcattgcggc gactaacgcc
    20461 atcagcgata tcttcgcgat ggggggcaaa ccgattatgg cgattgcgat cctcggctgg
    20521 ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa ccgaaggtgg acgctatgca
    20581 tgccgtcagg cgggtattgc gctggctggc ggtcactcca tcgatgcgcc ggagccgatt
    20641 tttggtctgg cggtaacggg gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca
    20701 caagccggat gcaaactgtt cctgacgaaa ccgctgggga tcggcgttct taccacggct
    20761 gagaaaaaat cactgttgaa accagaacat cagggactgg cgacggaagt gatgtgccgg
    20821 atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt
    20881 acgggctttg gtctgctggg ccacttgagc gaaatgtgtc agggggctgg tgtgcaggca
    20941 cgcgtcgact atgaagcgat cccgaaactc cccggtgttg aagagtacat taagttgggc
    21001 gcagtacctg gcggcactga acgtaacttt gccagctacg gtcatctgat gggtgaaatg
    21061 ccgcgtgaag tgcgcgatct gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg
    21121 gtcatgccgg aagcagaaaa tgaggttaaa actacagccg ccgagtttgg cattgaactg
    21181 acggcgattg gcgaactggt gccagcgcgc ggcggtcgtg ccatggttga gatccgttaa
    21241 tttaatgcgg ttgtttattg ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct
    21301 gcccaaaccg caccggaaag gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac
    21361 ttggtgtatc ggtcacctgc ttgagcaggc gcagccagac gcctacgaca gccgctatgc
    21421 gcgctggaat cttgcggatt tgccgattgt cccggaaaag tggcaattac agccacgacc
    21481 ctccgtgacc aaacaactta acgtcatcaa acgcttcctg catgaagcca gcgaaatcgt
    21541 tcacgccggg gacccggatc gtgaagggca attgctggtg gatgaagtgc tggactatct
    21601 gcaactggca ccggaaaagc gccagcaggt gcagcgttgc ttgataaacg acctgaaccc
    21661 gcaggcggtt gagcgggcga tcgaccgtct tcgttccaac agtgagtttg tgccattgtg
    21721 cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac ggcatcaata tgacccgtgc
    21781 gtataccatt ctcggtcgca atgccggtta tcagggcgta ctttccgtgg gacgcgtgca
    21841 gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag attgaaaact tcgtggcgaa
    21901 agacttcttt gaagtcaaag cacatatcgt gacacctgcc gatgagcggt ttaccgctat
    21961 ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg
    22021 tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa ccggctattg tcaccagcta
    22081 taacgataaa cgggaatcag aatccgcgcc gctgccgttt tcgctttcgg cgttgcagat
    22141 tgaagcggca aaacgcttcg gtctgagcgc gcagaacgtg cttgatatct gccagaagct
    22201 gtacgaaacg cacaagctaa tcacttatcc gcgttccgat tgtcgctatt tgccagaaga
    22261 acattttgcc ggacgccacg cggtgatgaa tgccatcagc gttcatgcac cggatctgtt
    22321 gccgcagcca gtggtagatc cagatatacg caaccgctgt tgggatgaca aaaaggtcga
    22381 tgcgcaccac gccatcatcc cgaccgcacg gagttctgcg atcaacctga cggagaacga
    22441 agcgaaggtc tataacctga ttgcccgtca atacctgatg cagttctgcc cggatgcggt
    22501 gttccgcaag tgtgttatcg aactggacat tgccaaaggc aaatttgtcg ctaaagcccg
    22561 ttttcttgct gaagcaggct ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa
    22621 cgacggcacg ccattgcctg tggtggcgaa aggcgatgag ttgctgtgtg aaaaaggtga
    22681 agtggtagag cggcaaaccc agccgccgcg ccattttacc gatgcaacac tgctttcggc
    22741 gatgaccggg atcgcgcgct ttgtgcagga caaagatctg aaaaaggtcc ttcgtgcgac
    22801 cgatggtctg gggacagaag caacccgtgc cgggattatt gaactgctgt tcaagcgtgg
    22861 ttttctgacc aaaaaagggc gctatatcca ctccaccgac gccggaaaag cgttattcca
    22921 ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg cactgggaat cggtgctgac
    22981 gcaaatcagc gaaaagcagt gtcgctatca ggactttatg cagccgctgg tggggacgct
    23041 atatcagctt attgatcaag ccaaacgtac gccggtgcgg cagtttcgcg gcattgtggc
    23101 tccgggcagt ggtggcagtg ctgataagaa aaaggctgca ccgcgtaaac gtagtgcgaa
    23161 aaaaagtccg ccagcagatg aagccggaag cggggcgata gcgtaagcga gcgaatcttt
    23221 cgtgctattc gagtcatatt ctgaaatatc cagcggatca agaaagttcg ttggatattt
    23281 ttttgcatgg ataaaattat cgcctctaaa gtatgtaata acagggaatg tgatgaaaaa
    23341 agttttattg caaaaccatc ctgggagcga gaagtattct tttaatggct ggggaatatt
    23401 taatagtaat tttgaacggg tgattaaaga aaataaggcc atgctgcttt gtaagtgggg
    23461 gttttattta acatgtgttg tcgctgtaat gtttgtattc gcagcgataa catccaacgg
    23521 tttgaatgaa agaggcctga ttaccgcggg atgctctttt ctttatctat taattatgat
    23581 ggggcttatt gttcgggccg gttttaaagc aaaaaaagaa caactgcatt attatcaggc
    23641 taaaggtatt gagccgctca gtatcgagaa gttacaggcg ctacaattga tcgcacctta
    23701 tcgattctat cataagcaat ggtctgaaac gctagagttc tggccgcgaa agcctgaacc
    23761 tggcaaagat accttccaat atcatgtgct tccttttgac tcgatcgata tcatcagtaa
    23821 aagacgcgag tctttagaga atcaatgggg tatcgaagat agcgaaagtt attgtgcctt
    23881 aatggagcat tttctttctg gcgaccatgg agccaatacc tttaaagcaa acatggagga
    23941 agccccagag caggttatcg ccttgttgaa taaatttgct gtttttccct cagactatat
    24001 ctctgattgc gctaataatc gctccggtaa atcctcggcg aagctaatat gggcggcgga
    24061 attatcatgg atgatctcga tatcaagcac agcttttcaa aacgggacaa ttgaagaaga
    24121 actggcctgg cattatataa tgcttgcttc tcgaaaggcg cacgagttgt tcgaaagcga
    24181 agaagattat caaaaaaata gtctaatggg atttctttac tggcatatct gctgctatcg
    24241 cagaaagtta acggatgctg aacttgaagc gtgttatcgt tacgacaagc agttttggga
    24301 gcactacagt aagaaatgcc gttggcccat aagaaatgtt ccctggggag catcatccgt
    24361 taaatactca taaacgcctg aaattttgcc gggggcgctg cgcttgaccg gcctacatta
    24421 gagcgtaact gcaatgaatt gtgcccatct gtaggcctga taagcgtagc gccatcaggc
    24481 atttacaact taaatcacac cctgcgccag catcgcatcg gcaaccttca caaaaccggc
    24541 aatgttcgcg ccctgcacgt agttggtttg ctcaccttca ccaccatgct caacacaggc
    24601 atggtggata tccagcatga tgtgatgcaa acgtgcgtca actttctcgg ctttccagcc
    24661 caggcgcgca gcgttttgtg ccatttccag gcccgacgtt gctacaccgc cagcattagc
    24721 cgctttaccc ggtgcaaaca gtacgcctgc ctgctggaac agttcagtcg cttcgatggt
    24781 ggtcggcata tttgcccctt cagcgacggc tttaacgcca ttggcgataa gctgatgcgc
    24841 ggcgtcaaca tccagttcgt tctgggtggc gcaaggcagg gcgatatcaa ccggtacaga
    24901 ccacggctgt tggccttcga gatagaccag accaaattct ttggcgtaat ctgccgctcg
    24961 accatcgcgg ctggctttga tttcgataag acgtgccagt ttctctttcg tgaatccgct
    25021 ttcatcaact acagtgccgc tggagtctga cgcagtgatc acacgagcac caaattccat
    25081 cgctttttcg atagcgtact gggcgacgtt gccggagcca gaaacggaaa cgctcatccc
    25141 ttcaaaaccc ataccgtggc gttttaacat tgcttctgtg aaataaacca gaccgtagcc
    25201 ggtagcttcc gggcgaataa gactaccgcc aaatgaaagg cccttaccgg tgaagacgca
    25261 ggcggtattg ttggagagct ttttcatcat ccccgccata aagccgactt cacgaccacc
    25321 aacgccgata tcacctgccg gaacgtcggt atccgcgccc aggtggcgat acagttcagt
    25381 catcagcgtc tggcaaaaac gcatcacttt accttcgctt tttcctttcg gatcgaaatc
    25441 gctgccgcct ttaccaccgc ccatcggcag ggtagtcagg gcatttttga aggtttgttc
    25501 aaagccgagg aatttgagaa tggaaaggtt aactgacgga tggaagcgca taccgccttt
    25561 gtacgggccg atggcagagc tgaactgcac acgccatgca cggttgacct gtatctggtt
    25621 gcgatcatca acccatacca cgcgaaactg gatcacgcgc tccggttcaa ccaga
//
LOCUS       DAJTNP010000054        25648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTNP010000054 DAJTNP010000000
VERSION     DAJTNP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..76
                     /gene="ldrD"
                     /locus_tag="OO160_002400"
     CDS             <1..76
                     /gene="ldrD"
                     /locus_tag="OO160_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5524490.1"
                     /translation="HDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(163..1842)
                     /gene="bcsG"
                     /locus_tag="OO160_002401"
     CDS             complement(163..1842)
                     /gene="bcsG"
                     /locus_tag="OO160_002401"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5524491.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1839..2030)
                     /gene="bcsF"
                     /locus_tag="OO160_002402"
     CDS             complement(1839..2030)
                     /gene="bcsF"
                     /locus_tag="OO160_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5524492.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2027..3598)
                     /gene="bcsE"
                     /locus_tag="OO160_002403"
     CDS             complement(2027..3598)
                     /gene="bcsE"
                     /locus_tag="OO160_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5524493.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3871..4059
                     /locus_tag="OO160_002404"
     CDS             3871..4059
                     /locus_tag="OO160_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5524494.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4071..4823
                     /gene="bcsQ"
                     /locus_tag="OO160_002405"
     CDS             4071..4823
                     /gene="bcsQ"
                     /locus_tag="OO160_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5524495.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4820..5179
                     /locus_tag="OO160_002406"
     CDS             4820..5179
                     /locus_tag="OO160_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5524496.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5176..7437
                     /gene="bcsA"
                     /locus_tag="OO160_002407"
                     /pseudo
     CDS             5176..7437
                     /gene="bcsA"
                     /locus_tag="OO160_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7448..9787
                     /gene="bcsB"
                     /locus_tag="OO160_002408"
     CDS             7448..9787
                     /gene="bcsB"
                     /locus_tag="OO160_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5524497.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9794..10900
                     /gene="bcsZ"
                     /locus_tag="OO160_002409"
     CDS             9794..10900
                     /gene="bcsZ"
                     /locus_tag="OO160_002409"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5524498.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10977..11318
                     /locus_tag="OO160_002410"
     CDS             10977..11318
                     /locus_tag="OO160_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5524499.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11233..11484
                     /locus_tag="OO160_002411"
                     /pseudo
     CDS             11233..11484
                     /locus_tag="OO160_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11566..13554
                     /gene="hmsP"
                     /locus_tag="OO160_002412"
     CDS             11566..13554
                     /gene="hmsP"
                     /locus_tag="OO160_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5524500.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13742..15028
                     /gene="dctA"
                     /locus_tag="OO160_002413"
     CDS             13742..15028
                     /gene="dctA"
                     /locus_tag="OO160_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5524501.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15249..16745
                     /locus_tag="OO160_002414"
     CDS             15249..16745
                     /locus_tag="OO160_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5524502.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16841..17770)
                     /gene="kdgK"
                     /locus_tag="OO160_002415"
     CDS             complement(16841..17770)
                     /gene="kdgK"
                     /locus_tag="OO160_002415"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5524503.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18002..18769
                     /gene="pdeH"
                     /locus_tag="OO160_002416"
     CDS             18002..18769
                     /gene="pdeH"
                     /locus_tag="OO160_002416"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5524504.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18839..20899
                     /locus_tag="OO160_002417"
     CDS             18839..20899
                     /locus_tag="OO160_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5524505.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21081..22403)
                     /locus_tag="OO160_002418"
     CDS             complement(21081..22403)
                     /locus_tag="OO160_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5524506.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22814..23827)
                     /gene="yhjD"
                     /locus_tag="OO160_002419"
     CDS             complement(22814..23827)
                     /gene="yhjD"
                     /locus_tag="OO160_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5524507.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23876..24775)
                     /locus_tag="OO160_002420"
     CDS             complement(23876..24775)
                     /locus_tag="OO160_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5524508.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25295..>25648
                     /locus_tag="OO160_002421"
     CDS             25295..>25648
                     /locus_tag="OO160_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5524509.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAA"
BASE COUNT         6097 a         6448 c         6977 g         6126 t
ORIGIN      
        1 gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg tgaactggct
       61 gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc
      121 ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg ggtaaggcac
      181 ccagtcgccg ccgttcaggc gaacgtacgg tttatcttgg tattgaataa ctactgcatt
      241 tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt tccagtcaac
      301 attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg agatagccag
      361 gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct tcatgccgaa
      421 gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac gtaggccaga
      481 tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac
      541 tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg
      601 cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc cgtcatgcag
      661 tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg taacgtccag
      721 ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat
      781 aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt cgcgaacttc
      841 tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg taaagcccag
      901 tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca gattagtgtg
      961 cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt
     1021 ggcggagtta aagttcttga aatcaatatc gaaatgcgac cacagtggat gcgacatcaa
     1081 cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca gtagttcaaa
     1141 tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg cctccgcgtt
     1201 atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg
     1261 catatcaccc actaccggtg cgccacccgt cgccgcaacg gttgccgctg cgttaccacc
     1321 cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac ttggtcccgc
     1381 cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa tgcgaatcca
     1441 ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca tctgccagtt
     1501 aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca cctgcgaacc
     1561 ctggctcatt atgctttccg ggccaggcaa ccagatgtca tgccagaaca aagcaaagcc
     1621 gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg taacgcggaa tgggcatcag
     1681 cagaaacgcg gcaaacacca aattgaggag cgggtggaag ttaagatatc ccgcccacaa
     1741 caggccaaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc agtattgcca
     1801 gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt tgccctggct
     1861 ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc aaagaacaaa
     1921 cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat
     1981 atcagtgcgc aaacgataat aatttcaatg atatcgctga tggtcatcat gatgagcgct
     2041 ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt
     2101 gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatt ccccattgtt
     2161 ctggggcaag caggcgcatc tgcaccagct cggcactgat ttgatcatct tcaaaccaga
     2221 ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc aacgcggtat
     2281 ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg ccaatggtca
     2341 tgatatcgcc ggtacggtta gggcgacata gcgtcagggc ttgctcaacg cggatacccg
     2401 gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggcaagaga gggttattca
     2461 tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc agtttgagcg
     2521 gctgggtcat tgacagcaag gtagtgatat cttccggtac atagcgacta aacttctgcc
     2581 cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac ggaataacca
     2641 tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta gcggtatttt
     2701 cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga atgctgcggg
     2761 ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg
     2821 tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt tccgatagcg
     2881 gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt
     2941 ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc tgaacgctaa
     3001 gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc agcaaatgtt
     3061 ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc tcaagcaaca
     3121 atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa caatggttta
     3181 acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca ggaatatttt
     3241 gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca atagaacact
     3301 gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa aataatttta
     3361 ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg ctgtccatgc
     3421 taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc gccagactga
     3481 tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc atatgtcgca
     3541 gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg tccctcatgt
     3601 caccattttg tctgattatc agtttactag cgaaagctaa gaaataaacc taccattgaa
     3661 attaaaactt atcagattta gcatgtgaat caatttagaa ggtcacaaga cgtgtgattt
     3721 tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag tgagcaaatt
     3781 cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg gttagaattg tctcatgaat
     3841 ggtacggtta tttcataggg atcaagcaaa atgaataaca atgaaccaga tactctgcct
     3901 gatcccgcga taggctatat cttccagaat gatattgtgg cgttaaagca ggcattttca
     3961 ctgcctgata ttgattatgc cgatatttcc caacgcgaac agttggccgc ggcattaaaa
     4021 cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg ggattggtga atggccgtac
     4081 tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac catcaccgcc gcattagcct
     4141 ggtcattaca aatgttggga gaaaatgtcc tggtggtcga tgcctgcccg gacaacttgt
     4201 tgcgcctgtc atttaatgtt gattttaccc accgtcaggg ctgggccaga gcgatgctgg
     4261 atggccagga ctggcgtgac gctgggttgc gctacacctc gcagctcgat ttgctgcctt
     4321 ttggtcagtt atccattgaa gaacaagaaa atccacagca ctggcaaacc cggctgagcg
     4381 atatttgctc cggcttacag caactaaaag ccagcgggcg ttaccagtgg attttaatcg
     4441 acttaccgcg tgatgcctcg cagataaccc accagctgct gagtttgtgc gagcactcgc
     4501 tggcaatcgt caatgtggat gccaactgcc atatccgact gcatcagcaa gcgctgccgg
     4561 atggcgcaca tattttgatt aatgacttcc gtattggcag tcaggttcag gacgatattt
     4621 accagctttg gttgcaaagc cagcgccgat tactgccgat gctcattcat cgtgatgaag
     4681 cgatggctga atgcctggcg gctaagcaac cagtaggtga atatcgcagt gatgcgctgg
     4741 cggctgaaga gatactgacg ctggcgaact ggtgcctgtt gaactactcc gggctgaaaa
     4801 cgccagtcgg gagtgcatca tgagtatcct gacccggtgg ttgcttatcc cgccggtcaa
     4861 cgcgcggctt atcgggcgtt atcgcgatta tcgtcgtcac ggtgcgtcgg ctttcagcgc
     4921 gacgctcggc tgtttctgga tgatcctggc ctggattttt attccgctgg agcacccgcg
     4981 ctggcagcgt attcgcgcag aacataaaaa cctgtatacg catatcaacg cctcgcgtcc
     5041 gcgtccgctg gacccggtcc gttatctcat tcaaacatgc tggttattga tcggtgcatc
     5101 gcgcaaagaa acgccgaaac cgcgcagcgg gcattttcag gtctgcaaaa tattcgtgga
     5161 cgttaccatc aatggatgaa cgagctgcct gagcgcgtta gccataaaac acagcatctg
     5221 gatgaaaaaa aagagctcgg tcatttgagt gccggggcgc ggcggttgat cctcggtatc
     5281 atcgtcacct tctcgctgat tctggcgtta atctgcgtta ctcagccgtt taacccgctg
     5341 gcgcagttta tcttcctgat gctgctgtgg ggggtagcgc tgatcgtacg gcggatgccg
     5401 gggcgcttct cggcgctaat gttgattgtg ctgtcgctga ccgtttcttg ccgttatatc
     5461 tggtggcgtt acacctctac gctgaactgg gacgatccgg tcagcctggt gtgcgggctt
     5521 attctgctct tcgctgaaac gtacgcgtgg attgtgctgg tgctcggcta cttccaggta
     5581 gtatggccgc tgaatcgtca gccggtgcca ttgccgaagg atatgtcgct gtggccgtca
     5641 gtggatatct ttgtcccgac ttacaacgaa gatctcaatg tggtgaaaaa taccatttac
     5701 gcctcgctgg gtatcgactg gccgaaagac aaactgaaca tctggatcct cgatgacggc
     5761 ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg tgaaatatat cgcccgcacc
     5821 actcatgaac atgcgaaagc gggcaacatc aacaatgcgc tgaaatatgc caaaggcgag
     5881 ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat cgttcctgca aatgaccatg
     5941 ggctggttcc tgaaagagaa acagctggcg atgatgcaga ctccacacca cttcttctcg
     6001 ccggacccgt ttgaacgcaa cctgggacgt tttcgtaaaa cgccgaacga aggcacgctg
     6061 ttctatggtc tggtgcagga tggcaacgat atgtgggacg ccactttctt ctgcggttcc
     6121 tgtgcggtga ttcgtcgtaa gccgctggat gaaattggcg gcattgctgt cgaaaccgtg
     6181 actgaagatg cgcatacttc tctgcgtttg caccgtcgtg gctatacctc cgcgtatatg
     6241 cgtattccgc aggcggcggg gctggcgacc gaaagtctgt cggcgcatat cggtcagcgt
     6301 attcgctggg cgcgcgggat ggtataaatc ttccgtctcg ataacccgct caccggtaaa
     6361 ggactgaagt ttgctcagcg gctgtgctac gtcaacgcca tgttccactt cttgtcgggc
     6421 attccacggc tgatcttcct gactgcgccg ctggcgttcc tgctgcttca tgcctacatc
     6481 atctatgcgc cagcgttgat gatcgcccta ttcgtgctgc cgcatatgat ccatgccagc
     6541 ctgaccaact cgaagatcca gggcaaatat cgccactctt tctggagtga aatctacgaa
     6601 acggtgctgg cgtggtatat cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa
     6661 ggcaaattta acgtcaccgc caaaggtgga ctggtggaag aagagtacgt cgactgggtg
     6721 atctcgcggc cctacatctt ccttgttcta ctcaacctgg tgggcgttgc ggtaggcatc
     6781 tggcgctact tctatggccc gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg
     6841 gtgttctaca acctgattgt tcttggcggc gcagttgcgg tatcggtaga aagcaaacag
     6901 gtacgccgat cgaaccgcgt ggagatgacg atgcccgcgg caattgcccg cgaagatggt
     6961 cacctcttct cgtgtaccgt tcaggatttc tccgacggtg gtttggggat caagatcaac
     7021 ggtcaggcgc agattctgga agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag
     7081 gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta atgaagttgg gctgaaatta
     7141 atgccgctca ccacccagca acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat
     7201 acatgggcgc tctggcagga cagctacccg gaagataagc cgctggaaag tctgctggat
     7261 attctgaagc tcggcttccg tggctaccgc catctggcgg agtttgcgcc ttcttcggtg
     7321 aagggcatat tccgtgtgct gacttctctg gtttcctggg ttgtatcgtt tattccgcgc
     7381 cgcccggagc ggagcgaaac ggcacaacca tcggatcagg ctttggctca acaatgatga
     7441 taacgcgatg aaaagaaaac tattctggat ttgtgcagtg gctatgggga tgagtgcgtt
     7501 cccctctttc atgacgcagg cgacgccagc aacgcaacca ctgatcaatg ctgagccagc
     7561 tgtagccgcc cagacggaac aaaatccgca ggtggggcaa gtgatgccag gcgagcaggg
     7621 agctgatgcc cccatcgtgg cgcagaacgg tccttcgcgt gatgtgaagc tgacctttgc
     7681 gcaaatcgcg ccgccgccgg gcagcatggt gctacgtggt attaacccga acggcagcat
     7741 tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg atgctcaact tcgaatacac
     7801 gccatcgcca tcgttactgc ctgtccagtc gcagttaaag gtttatctca atgatgagct
     7861 gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa aaaacgctgg cgcaaatgcc
     7921 cattaatcca ctgtttatta ccgacttcaa ccgtgtgcgg ctggagtttg tcggccatta
     7981 tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg ctggatgttg ggcggagcag
     8041 tggactggat ctgacctatc agaccctgaa tgtgaagaat gacctgtcac acttcccggt
     8101 gccattcttt gacccgcgcg ataaccgtac taacaccttg ccgatggtct ttgcgggtgc
     8161 gccggatgtt gggctgcaac aagcatctgc cattgtcgcc tcgtggtttg gttcgcgttc
     8221 tggctggcgt gggcagaact tcccggtgct ctataaccaa ttgccggatc gcaatgccat
     8281 tgtctttgca accaacgaca aacggccgga cttcctgcgc gatcatccgg cggtaaaagc
     8341 cccggtgatt gagatgatta accatccgca gaatccttac gtcaaactgc tggtggtgtt
     8401 tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt atcgctcagg gtaacattct
     8461 gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg ctgctaccgc gtaagccgta
     8521 cgatgcgccg aactgggtac gtaccgatcg tccggtcaca tttggcgaac tgaaaaccta
     8581 tgaagaacag ttacaatcca gcggccttga gccagcagcg attaacgttt cgctaaacct
     8641 gccgccggat ctctacctga tgcgcagtac cggcattgat atggatatta attaccgcta
     8701 caccatgccg ccggtgaaag acagttcgcg gatggatatc agcctgaata accagttcct
     8761 gcaatccttc aacctgagca gcaaacagga ggcgaaccgt ctgctgctgc gtattccggt
     8821 attacaaggt ttgctggatg gcaaaacaga tgtctctatt ccggcgctga aactgggcgc
     8881 gaccaaccag ctgcgcttcg actttgagta tatgaacccg atgccaggcg gttcggtaga
     8941 taactgtatt accttccagc cggtgcagaa tcatgtggtg attggtgacg attccaccat
     9001 cgacttctcg aagtattacc acttcatccc gatgccggat ctacgcgcct ttgctaacgc
     9061 gggcttccca ttcagccgga tggcggatct gtcgcaaacc atcaccgtga tgccgaaagc
     9121 gcctaacgaa gcacagatgg aaacgttgct gaatactgtt ggttttatcg gcgcacagac
     9181 gggcttcccg gcgattaatc tgacggtgac cgatgatggc agcaccattc agggcaaaga
     9241 tgccgacatc atgatcatcg gtggtatccc ggacaaactg aaagacgata agcagatcga
     9301 cctattggtg caggcgaccg aaagctgggt gaaaacaccg atgcgccaga ccccgttccc
     9361 cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc cagtcaacgc tgacctcttc
     9421 cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat
     9481 tgcgctgttg gcagatagcc cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag
     9541 cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag
     9601 cctacgtgtt ggcgacgttt attacgtagg tcatctgccg tggttcgagc gcttgtggta
     9661 tgcgctggca aaccatccga ttctgctggc ggtgctggcg actatcagtg tgatattgct
     9721 ggcatgggta ctgtggcgtc tgctgcgaat tattagtcgt cgtcgtctta acccggataa
     9781 cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg acgatgctgt tgttggctgc
     9841 ctttagtgtt caggcagcct gtacctggcc tgcctgggag cagtttaaaa aggattacat
     9901 cagtcaggaa gggcgcgtca tcgaccccag cgacgcgcgc aaaatcacca cctccgaagg
     9961 gcaaagttac ggtatgttct ttgccctggc ggctaacgac cgtgcagctt tcgataatat
    10021 tctcgactgg acgcagaaca atctcgctca gggttcttta aaagaacgtt tgcccgcctg
    10081 gctgtggggc aagaaagaga acagtaagtg ggaagtgctg gacagcaatt cggcctccga
    10141 tggtgatgtc tggatggcct ggtcgttgct ggaggcgggg cgtttgtgga aagagcagcg
    10201 ttataccgac atcggcagcg cgttgctaaa acgtatcgcg cgggaggaag tggtgacggt
    10261 gcctgggctg ggttccatgt tgttaccggg caaagtgggt tttgctgagg ataacagctg
    10321 gcgttttaac cccagctaca tgccgccgac gctggcgcag tatttcaccc gctttggcgc
    10381 gccgtggacc acgctgcgcg aaaccaatca acatttattg ctggaaaccg ccccgaaagg
    10441 cttttcgcca gactgggtgc gctatgagaa agacaaaggc tggcagctaa aagccgaaaa
    10501 aacattgatc agcagctacg acgctatccg cgtttacatg tgggtaggca tgatgcctga
    10561 cagcgatccg caaaaagcgc ggatgctcaa ccggtttaaa ccgatggcga cattcactga
    10621 gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg gggaaagcgc agggtaaagg
    10681 accggtcggt ttttctgccg ccatgctgcc ttttttacaa aaccgcgatg cgcaggcagt
    10741 tcagcgccag cgcgtggccg ataactttcc cggcagcgat acctattaca actatgtgct
    10801 gaccctgttt ggacaaggct gggatcaaca ccgtttccgc ttctcgacaa aaggtgagtt
    10861 attacctgac tggggccagg aatgcgcaaa ttcacactaa acatattcac gctttccctc
    10921 ggtctggccg tcatgccgat ggtcgaggca gcaccaacca tcagcagacc ctgcggatgg
    10981 acagcggcaa cactaatgcc gtgcgcgggc tggcgaatat ttaccgccag cagtcgccag
    11041 aaaaagctga agcgtttatc gcctcgctct ctgccagcca gcggcgcagc attgatgata
    11101 tcgaacgcag cctgcaaaac gaccgtctgg cacagcaggc agaggtactg gaaaaccagg
    11161 gcaaatgggc gcaggcggca gcacttcagc ggcaacgact ggcgctgggc cccggcagcg
    11221 tatggattac ttaccgactg gcaggaagaa gctgcgcggc aatccaacga tggcggcagc
    11281 agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac ttccaactgg
    11341 tttgttggca cggcaattga tatccagcag gtgaaagatt acgcacccag ccatttcctg
    11401 ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc gccgcagccg
    11461 ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg ccgctgcgat
    11521 cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg cgtaagtcgc
    11581 tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt cgtttttatt
    11641 ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac ggctacgcaa
    11701 ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt gaaaggcgat
    11761 attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt cagccgtgcc
    11821 gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc agagcgtccg
    11881 gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct gggcgtctac
    11941 tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca ggcggattcc
    12001 ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat ttacttactt
    12061 ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat tttgcatccg
    12121 ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt tggtcaccaa
    12181 ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg cagttacaac
    12241 ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc gatgcgcttc
    12301 ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca ggttgtcgcg
    12361 cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga tactgcgggc
    12421 gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa actcaaatcg
    12481 gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc tgtcattgcc
    12541 aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact cactatcatg
    12601 agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat tggcgtggcg
    12661 atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc tgcggcattt
    12721 accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca gatggaagca
    12781 gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa tcatcagttt
    12841 gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag tgcggaagtg
    12901 ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt aatcgatcgc
    12961 attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga gtcctgtcga
    13021 ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa cctctctgcg
    13081 ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac ccgctatcgc
    13141 attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga cgaccctcat
    13201 gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc gctggatgat
    13261 ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt gccaatcgac
    13321 gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag catgattgct
    13381 gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg cgtggagact
    13441 gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg cttccttttt
    13501 gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa gtagctaccc
    13561 caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca aactttttaa
    13621 cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg tgttatttta
    13681 aggccgcagg tacccccccc cataacctta caagacctgt ggttttacta aaggacaccc
    13741 tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg
    13801 tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg
    13861 cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg
    13921 cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta
    13981 ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca
    14041 gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta
    14101 cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag
    14161 cgtcattggc gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt
    14221 tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg tgatcgaaag
    14281 tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc
    14341 gctcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg
    14401 gcagctgatt atctgtttct acattacctg tatcctgttt gtggtgctgg tattgggttc
    14461 aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact
    14521 gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat
    14581 ggagaaactc ggctgccgta aatcggtggt ggggctggtc atcccgacag gctactcgtt
    14641 taaccttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac
    14701 taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc
    14761 taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc
    14821 ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc
    14881 agaagctcgt gcgctaacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa
    14941 gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga
    15001 tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg
    15061 agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca
    15121 ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt
    15181 taacgtcatt tagactgacc gacaacgggc agttttttta ttaccaggat agttgatcag
    15241 gggttcacat gcagggcata aaaattcgac ttttagcggg cggtttgctg atgatggcca
    15301 ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc
    15361 tttccaacgg tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg
    15421 aaattcgcct gctggttaat accggttcgc tcgccgaaag tacacaacag agcggttaca
    15481 gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc
    15541 gttcattgtg gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt
    15601 atgacaccac gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag
    15661 cgctctctta tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc
    15721 acgcgctgca aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt
    15781 ggcgctatcg tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac
    15841 aacccgttga agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa
    15901 tgacgctact ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa
    15961 cgtttggcga actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc
    16021 tgcgtgcgga agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca
    16081 tgtgggatac gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg
    16141 cggacctggc ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtgaca
    16201 gcaaagacat cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca
    16261 tcaacatcga atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac
    16321 tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa
    16381 agaaactgga gctgcagaaa ctgtttgccg cctatgcacg ggctgatacc gatattctga
    16441 tgggccagcg gatgcgttcg ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc
    16501 agaaactgcg gcaggatttc cttaacagcc tgactgtgga gatgttaaat caggatctgc
    16561 gtcagcagtt gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat
    16621 ttaacatgaa agcgttgcag gcggcctggg atcaaatcat ggccccatct accgccgctg
    16681 cgaccacctc tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac
    16741 agtaatgcct gatggtgatg agttagcaca accatcaggt atttatggat gagctggata
    16801 gaggggtaac actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac
    16861 gcgggataat cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg
    16921 ccgcgttttc cgcactgccg cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt
    16981 cgccagctgc ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcaa
    17041 ctaacgcttc accagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt
    17101 ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca
    17161 gcgcgtcttc gtcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgctggt
    17221 acacctgctg tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa
    17281 tcacttttcc gcctttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg
    17341 tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct
    17401 cttcgcaaat cgccgcagac tgctcgctcg ccagccagaa tttggcggcg gcttcgttac
    17461 gccagtagta gaacgtacgt tcgccggtgc tgtcggtttc aatgtagtaa aggcccggaa
    17521 gacggttttc catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca
    17581 gcatctgctg gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg
    17641 caggatcgac ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc
    17701 gcttaacgtc cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct
    17761 ttttggacat agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg
    17821 tttgctggtc aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc
    17881 tgaaagattc cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct
    17941 tacgcgccga taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt
    18001 gatgataagg caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca
    18061 ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac
    18121 atgcgggcgg ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc
    18181 gcaacgcctg ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt
    18241 tgtgaaagag cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacggcct
    18301 gctggcatcg gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat
    18361 cctgcgccag attgagcgtc ttccctggct gcgtttcgaa ctggtggagc atatccgtct
    18421 gccgaaagat tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt
    18481 tggtaccggg atggcaaatt tctctgcact aagtgaagtg cgttatgact acatcaaaat
    18541 cgcgcgagaa ctgtttgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca
    18601 gcttttacat ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc
    18661 ggaagagtgg cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc
    18721 acgcccggca ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt
    18781 ttccgccggg ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat
    18841 gagcaaggca ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt
    18901 ggcgatcatt ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa
    18961 agtctctgcg gagttgaatc gtccgttcgc tatccgtggc aatctgggcg tggtgtggga
    19021 gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga
    19081 agacatcatt cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg
    19141 agtagaggca acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat
    19201 caagctcgaa aagcccgacg cgcgcctgat tcgcctctct gaaaagaaca ataactggac
    19261 gtttaatctt gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt
    19321 tcggctggat aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa
    19381 agcggatctg gaaatttttg ttgatccgtt aggcaagccg ctgccattca gcgaagttac
    19441 tggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat tacatttttg gcctgaaggc
    19501 gcagggacgt tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc
    19561 gctgcgtggc gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg
    19621 tgttgctttt gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct
    19681 taaattttct ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga
    19741 taccccgccg tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc
    19801 ggtctttgat tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct
    19861 ggtctacacc accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt
    19921 gcggctggcg gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc
    19981 gaaacggtct gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc
    20041 ttatgaccgc ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg
    20101 gcggcgcatt gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct
    20161 caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc
    20221 ggcgaatatt catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc
    20281 tcgtcgactg aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg
    20341 ggaaatgaac ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg
    20401 caacagtaac ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat
    20461 ggagattgtt gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga
    20521 ggtgcgggtg aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca
    20581 gatttttgct ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc
    20641 ttcggaacag ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact
    20701 gcgttcgccg ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg
    20761 accgctgatt gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccggcggc
    20821 ggcgttactg gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt
    20881 gtcgcagatg aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat
    20941 ttagattttt ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg
    21001 cctacaaatc cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg
    21061 catcaggctt ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg
    21121 gcgattaacg tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag
    21181 ttagcctgca accaggctgc gatatacggc gcaacggaag ccccgagaat cgacgatacg
    21241 ttgtaagaga acgatgctcc ggtgtaacgc acttctgtcg gaaacagctc tggtaacagc
    21301 gcacccatcg gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa
    21361 accagaatcg ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg
    21421 atcagcgtgg tgatgatcac catgctttta cggcgaccaa aggcatcagc cagtaatccg
    21481 gcgactggca ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg
    21541 ttacgcggca ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc
    21601 gtcatgatgt aaaacagcgt ataggttgcc agcataatga acgtacccag tacggttacg
    21661 cgaacatgtt tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg
    21721 actttctcaa acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc
    21781 agcaccgccg agaagataaa tggcacacgc cagccccagc tcataaactg ctcatcggtc
    21841 agtagccagg aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgcct
    21901 agctgtggga aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc
    21961 agcgccgcgc cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc
    22021 agcagcagtg gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg
    22081 gtcgaaatcc ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg
    22141 ccaaaatgac caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg
    22201 gcgagcgact gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac
    22261 acaataacgg ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg
    22321 agagaggcga caaggacttt attacgcgag ttgatcggcg tgtattcttg ttcgtggtcg
    22381 agtgttgtgg ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat
    22441 tacgcacagc aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag
    22501 gcaaaaaggg gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt
    22561 ttataaatag tggataaaac tggtttcagg ttaaataaaa gttaccaact ggatttatat
    22621 gctggggtta tgagttggag tgaaattgtt tgctcccaaa tagatggctg aagaggctct
    22681 ggggaagagt ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt
    22741 atccggccta cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg
    22801 catccggcct attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg
    22861 cggtggcaat ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca
    22921 tcagccccag cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg
    22981 tgtaggtcat cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa
    23041 tcagggcttt tttgcgcgga cggtggcgcg gcaaccgcca gaagatccag aagaaaagca
    23101 gatagttggc gaagatggaa atcgccaggc caatcaatcg ccacgtcggt ttcagccact
    23161 caatgctgtt caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg
    23221 tgatcgaaag cgtcacaatc agcgcaatca acaaaccaat cagtgaaata aaatcacgca
    23281 gatattttac ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact
    23341 gggcacgaat cgcttcacgc aggttaccca tccagttgat gccggaataa agtgccaccg
    23401 ccaggccgac aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca
    23461 acgtggcggc tagtgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta
    23521 gcaacatcgg atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg
    23581 ggatcatcga caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat
    23641 cgttaaagcg ttctgtcgcg cgaataaggt gcgcaataac cggttggcgt tggacttttt
    23701 cggcagtggt ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat
    23761 ccaactgctt aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct
    23821 gcgtcatccg ctcttttgtc cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt
    23881 ccaggtgctc tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa
    23941 gctcccgacg ctgcgggtaa accagcgaaa gggagagcgg ctcggcacgg tagcctggca
    24001 acacttcaat aagccgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa
    24061 taatccccag accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca
    24121 tgccgccaga cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat
    24181 gtacacccag gtgcggtgta taacgcacta ttgcgtgtga agtcagatca tcggggcttt
    24241 gcggataacc aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gttagtttgc
    24301 cgaggggacg ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa
    24361 aaccatcatg aagaatatct accggacggt tatggctact cagttccagc tcaattcccg
    24421 gatgctgata gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg
    24481 ggatgtcgat gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca
    24541 gttcgctgag attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg
    24601 tgagtttgac ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg
    24661 cctgtatctg gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa
    24721 aactttccag ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt
    24781 tgaccgattg ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt
    24841 ctaacatcta atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag
    24901 gaaaagggcg gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca
    24961 gtgattccct ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat
    25021 tgccatttgt tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa
    25081 ccaccaatat aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca
    25141 ggcgtttagc tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat
    25201 ggccaccgcc aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta
    25261 agtcttttta acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca
    25321 gtcaatattt attcatggaa taaaaatcag tttacagcag cgtattccag gagtgagtat
    25381 tcagggggtc agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt
    25441 agttatgctc gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt
    25501 ggtgcagttt cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt
    25561 acaggaagtt atccatttta atgtgctggc cattgtgcct cgcgattcaa ccgtcgagac
    25621 atttgcgttg gcggtaaata gtgcggcg
//
LOCUS       DAJTNP010000055        25027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTNP010000055 DAJTNP010000000
VERSION     DAJTNP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..499
                     /gene="tnpA"
                     /locus_tag="OO160_002422"
                     /pseudo
     CDS             1..499
                     /gene="tnpA"
                     /locus_tag="OO160_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            827..1909
                     /locus_tag="OO160_002423"
     CDS             827..1909
                     /locus_tag="OO160_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5524510.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2398..3196)
                     /locus_tag="OO160_002424"
                     /pseudo
     CDS             complement(2398..3196)
                     /locus_tag="OO160_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3206..3757)
                     /gene="ybcL"
                     /locus_tag="OO160_002425"
     CDS             complement(3206..3757)
                     /gene="ybcL"
                     /locus_tag="OO160_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5524511.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3926..4258)
                     /gene="emrE"
                     /locus_tag="OO160_002426"
     CDS             complement(3926..4258)
                     /gene="emrE"
                     /locus_tag="OO160_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5524512.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4603..4917)
                     /gene="fliE"
                     /locus_tag="OO160_002427"
     CDS             complement(4603..4917)
                     /gene="fliE"
                     /locus_tag="OO160_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5524513.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5131..6790
                     /gene="fliF"
                     /locus_tag="OO160_002428"
                     /pseudo
     CDS             5131..6790
                     /gene="fliF"
                     /locus_tag="OO160_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6783..7778
                     /gene="fliG"
                     /locus_tag="OO160_002429"
     CDS             6783..7778
                     /gene="fliG"
                     /locus_tag="OO160_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5524514.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7771..8457
                     /gene="fliH"
                     /locus_tag="OO160_002430"
     CDS             7771..8457
                     /gene="fliH"
                     /locus_tag="OO160_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5524515.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8457..9830
                     /gene="fliI"
                     /locus_tag="OO160_002431"
     CDS             8457..9830
                     /gene="fliI"
                     /locus_tag="OO160_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5524516.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9852..10193
                     /gene="fliJ"
                     /locus_tag="OO160_002432"
                     /pseudo
     CDS             9852..10193
                     /gene="fliJ"
                     /locus_tag="OO160_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10190..11317
                     /gene="fliK"
                     /locus_tag="OO160_002433"
     CDS             10190..11317
                     /gene="fliK"
                     /locus_tag="OO160_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5524517.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11422..11886
                     /gene="fliL"
                     /locus_tag="OO160_002434"
     CDS             11422..11886
                     /gene="fliL"
                     /locus_tag="OO160_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5524518.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11891..12895
                     /gene="fliM"
                     /locus_tag="OO160_002435"
     CDS             11891..12895
                     /gene="fliM"
                     /locus_tag="OO160_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5524519.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12892..13305
                     /gene="fliN"
                     /locus_tag="OO160_002436"
     CDS             12892..13305
                     /gene="fliN"
                     /locus_tag="OO160_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5524520.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13308..13673
                     /gene="fliO"
                     /locus_tag="OO160_002437"
     CDS             13308..13673
                     /gene="fliO"
                     /locus_tag="OO160_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5524521.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13673..14438
                     /gene="fliP"
                     /locus_tag="OO160_002438"
                     /pseudo
     CDS             13673..14438
                     /gene="fliP"
                     /locus_tag="OO160_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            14448..14717
                     /gene="fliQ"
                     /locus_tag="OO160_002439"
     CDS             14448..14717
                     /gene="fliQ"
                     /locus_tag="OO160_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5524522.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14726..15511
                     /gene="fliR"
                     /locus_tag="OO160_002440"
     CDS             14726..15511
                     /gene="fliR"
                     /locus_tag="OO160_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5524523.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15796..16419
                     /gene="rcsA"
                     /locus_tag="OO160_002441"
     CDS             15796..16419
                     /gene="rcsA"
                     /locus_tag="OO160_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5524524.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16463..16651)
                     /gene="dsrB"
                     /locus_tag="OO160_002442"
     CDS             complement(16463..16651)
                     /gene="dsrB"
                     /locus_tag="OO160_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5524525.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16814..17041
                     /gene="yodD"
                     /locus_tag="OO160_002443"
     CDS             16814..17041
                     /gene="yodD"
                     /locus_tag="OO160_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5524526.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17339..18154
                     /locus_tag="OO160_002444"
                     /pseudo
     CDS             17339..18154
                     /locus_tag="OO160_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18151..19845)
                     /gene="yedQ"
                     /locus_tag="OO160_002445"
                     /pseudo
     CDS             complement(18151..19845)
                     /gene="yedQ"
                     /locus_tag="OO160_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20016..20198)
                     /locus_tag="OO160_002446"
     CDS             complement(20016..20198)
                     /locus_tag="OO160_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5524527.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20277..21194)
                     /locus_tag="OO160_002447"
     CDS             complement(20277..21194)
                     /locus_tag="OO160_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5524528.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21367..22287
                     /gene="yedA"
                     /locus_tag="OO160_002448"
     CDS             21367..22287
                     /gene="yedA"
                     /locus_tag="OO160_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5524529.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22276..22746)
                     /gene="vsr"
                     /locus_tag="OO160_002449"
     CDS             complement(22276..22746)
                     /gene="vsr"
                     /locus_tag="OO160_002449"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5524530.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22727..24145)
                     /gene="dcm"
                     /locus_tag="OO160_002450"
     CDS             complement(22727..24145)
                     /gene="dcm"
                     /locus_tag="OO160_002450"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5524531.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24212..24752)
                     /locus_tag="OO160_002451"
                     /pseudo
     CDS             complement(24212..24752)
                     /locus_tag="OO160_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24792..>25027)
                     /locus_tag="OO160_002452"
     CDS             complement(24792..>25027)
                     /locus_tag="OO160_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524532.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6320 a         6425 c         6174 g         6108 t
ORIGIN      
        1 gttgaaatgg atggcgaacc agatcacgtc catctgttaa ttaactatcc ccccaaactg
       61 gcgatatcca gtctggtaaa caaacagcct caaaggcgta tcgggtaggt tactgcgacg
      121 ggatcgacca gatattgctg ccaggtatta ccgcaaagat gttttgtggc gtcccggcta
      181 ttttgccagt agctgcggag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca
      241 gcaaacacct ggtcagatga aaaaacgagc cttatatccc cgccctgaag gacgggcttt
      301 tacggtgcac tggataagcc tggagattat ctacacctgc atggacatca ttgtacgatg
      361 ccccgtacat gctccatcac atcctcaaca tcaaagtatt agagccttgc aaaccattat
      421 gcgtctcagt ttgtctcatc tatcttacgc aaaaaaaata gaactaatat caagcggcgc
      481 tatattgcgt attaaataaa accacctcaa ttacattaat aaatctaaat aaatatcatg
      541 caaataattt ccatggttat ttttaggcta tttaaattaa aaaaataaat tatgagtcaa
      601 tcataaataa caacctttaa tataatttga ctaccaaatt tatattcgtt cacaaaatag
      661 tcatgcattg tgtgcaatag aaacagttca gataaaaata gggatctact tcacaattta
      721 aatgagaaac taaaacttac atcttgaaat aatctcattg attggatgaa tatttgtagt
      781 gcagtgacat catttttaaa taatagttca aataaaaggg ctcacgatga aaaaattaac
      841 agtggcaatt tctgctgtag ctgcatcagt actgatggcg atgtctgctc aggcagcaga
      901 gatttacaat aaagacagta acaagctgga tctgtacgga aaagttaatg ctaagcacta
      961 cttctcttct aacgatgcag atgatggcga cacaacttat gttcgtctgg gcttcaaagg
     1021 cgaaactcaa atcaacgatc aactgactgg tttcggtcag tgggaatatg aattcaaagg
     1081 caaccgtgct gaatctcaag gttcttccaa agataaaacc cgtcttgcct tcgctggcct
     1141 gaaattcggt gactacggct ccatcgatta tggccgtaac tacggtgtag catacgatat
     1201 cggtgcatgg accgacgttc tgccagaatt cggtggcgat acctggaccc aaacagatgt
     1261 gttcatgact ggtcgcacca caggtgttgc aacctatcgt aacaacgact tctttggtct
     1321 ggttgatggt ctgaactttg ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac
     1381 tgaagctaac ggtgatggtt tcggtttctc cactacttat gagtatgaag gattcggtgt
     1441 aggtgcaacc tatgctaaat ctgaccgcac taatgatcag gttatctacg gtaacaacag
     1501 cctgaatgca tctggtcaaa atgctgaagt atgggcagct ggtctgaaat atgatgcgaa
     1561 caacatctat ctggctacca cctattctga aacccagaac atgactgttt ttggtaataa
     1621 ccatattgcc aacaaagcac aaaacttcga agtagttgca caatatcagt tcgacttcgg
     1681 tctgcgtccg tccgttgctt acctgcaatc taaaggaaaa gacttgggtg cgtggggtga
     1741 tcaggacctg attgaatata ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc
     1801 cacttttgtt gattacaaaa tcaacctgat tgataagagc gatttcacga aagcatctgg
     1861 cgttgctacc gatgatatcg ttgctgtagg attggtttac cagttctaat ctgattactc
     1921 aaagatatgt tgtgggaggc tttgcctccc caacatataa gtggcgctct caagccactt
     1981 tccttagaag cactatcttg cttctgacta tataaattac cctttatttt ctgggacgtt
     2041 tcaacgcccc atttttaata atactcagtg aacaattaac gtatcaatta gatttaataa
     2101 caacgatatc cgtacctaac ctgatatcta atgtcataag catcccttta attatcccct
     2161 caacctcttg taaatttccc actcttgagc tccacatagc ccaaacgccg gatgcaaaaa
     2221 aaatcatacg tgcatctctg aaattgtgat ggaggaagtg agtatcaatt atatgtgctt
     2281 ataaacgttc ccgattggca cagaagtcac cggattgttc aggctccaat aggttgagta
     2341 tgtcgtattt attttccaaa ataaaaatcg atgctgttgc tccttaataa aatactttta
     2401 ctctctggac gcttttctta gctctcttgg tgttatatta aaacgattat ggaacctttc
     2461 agtaaaacga gaaggacact tgtaaccatt ttccctggca atctcgctga tgggttttac
     2521 tgtcgtttgt atagcaggac aacgcattat ttaacctcac gtcgtccaat atactttgaa
     2581 aacttattcc ctcgcttgcc agacggcgat gcaatgtgga aacagaaatg tagagatatc
     2641 tcgctacctt gtttgctgtc cattttgttc taggttcgga tagcattagg ttataacaac
     2701 gacttattaa tgattgttta ctatctgata aaagtaaaca attaacatga tgtgctccta
     2761 atgaaagtag aacgcccatt gctaagtgtt cctgaatttg cgttgagaag cctcgggaaa
     2821 tagatgtttt tagttgctcc caacaatata ttaactcggg gttctgaggt aagcagaaac
     2881 ctgtcgtgtt acgaatttga tcagttatcg cataaaattt ttgaaacttt tcaattagat
     2941 caatgggata ataaagcatt tctgcaagat aaagacctgc ttctggataa ttcacaatat
     3001 aaaattcata tccacaggga aataatatta tctgattatt atcaacagtt agactatgcg
     3061 tctcccaatt gataactttt tttccctgac ggatacgaca taaagctggc ataagaggct
     3121 taaccctgtg aatctcatga tgtttatgca tccgtatttc ttcaatcttt aggttagact
     3181 tatctcttgc cagcatactc ttaccctatt ttatctcata aactggtgtt atctcagcgg
     3241 ttgcaatttt attggcatta agcatataac caactaacgc tccgctggaa ttagaatcca
     3301 caggaatctt atcagttttt agagcccata ctttaaactg gtaatgatgg ggtttatctc
     3361 ctttgggagg acatgctcca ccaaatcctg cataaccaaa atcatttcgg ccttgaacag
     3421 caccagtcgg aagttttgta ccatcacgtc ttcctgcatc agtgggcaaa tatgttacag
     3481 ttgctggaat attagcaaca gtccagtgcc accatccact gcctgtagga gcatctggat
     3541 catacacagt tacggcaaag cttttggtgc cttcaggagc accagaccag gttaatgagg
     3601 gcgatgtatt accaccttca cacccaaatc cagaaaatac atgagacgtt gtaagttgct
     3661 ctcctgtttt tatttcatta ctagtgacct gaaatgctgc ggcctgcgca gaaaatgtta
     3721 tgaatgccaa tacacttgaa acgataagtt ttttcataaa aacctcattg ttgtgaccta
     3781 tcgttatttt atttgatgtg ccttgatctc attatgcata aaggcgcaat gatcgcgcaa
     3841 atgcaatcat aattataaag gtgccccgaa cattagccat aatatgcaca aatcagattc
     3901 ctgttatctc aaggagaatt attttttaat gcggtgcact tcgtgacaac aagttaatga
     3961 ctaacacacc ggcacaaatc aacatcatgc ctatgatagc tggcaggtcc agtcgttgac
     4021 caaaaaatcc ccaggaaagc aaactaatca gaacgatacc gactcctgac cagatagcat
     4081 aagcaatccc tgtaggtata taagccagcg tctgagctaa taaccagaat gatgcacaat
     4141 agcaaataat tgtgccaaca gagggccata accgtgtaaa cccctctgaa aacttcatta
     4201 aggttgtacc aatgacctct gcaagtattg cgccaccaag ataaatataa gggttcatag
     4261 catattcttt cctgttcata ctggagagaa ttgtactaca gtttgaacac aactcaccgt
     4321 tttcatcctg gtgtatgtgt atccaagggg ctctggcgtg gcaagagata aaggcaggaa
     4381 caataatgat cagaaggaac cccaggctaa cgaatgagtt cactcttcct gggagcaaag
     4441 cgaagacaaa acatgttatc gcgtttacgg atcgtggatg gcagaaaaca accaggatca
     4501 ggtatccatt ttaaaccaga aattgagtga gtttgcccca tccatgcccc acgctgtggg
     4561 atcggatata attaaacaag catagtcaac aggttacaca acctacacct gcatggacat
     4621 cacttcctga tacgccgcca ccagcttatt acgcacctga atccccattt gcatagaaac
     4681 tgaggctttt tgcatatcgg tcatcacatc gtttagcgcc acgccgggtt caccgagggt
     4741 gaatttttct gcctgcgtgc gggcgactgt ttgtgtatcg cttatgcgat cgagcgcggc
     4801 gtgcagctgc ccggcaaaac taatggtcgg ttgcggcagt gattcctgcg cacgcgcact
     4861 catcgccgta gcctgtaact ggctgataac cccttcaatc ccctgtatcg ctgacatttt
     4921 cgtctcccgg ataatttctg gtaacaaagc ctaccagtaa gtcaataaga caaaggcgct
     4981 aaatagcaac aaaaaaactg gtttattggc ggatagaaaa aacgaaagca caaataatgg
     5041 gagcgtcaat ttttcgagtg tgctgacccg agagtgagtc ttgttccact ttgccaataa
     5101 cgccgtccat aatcagccac gaagtgcgcg atgaatgcga ctgcagccca gacaaaatct
     5161 cttgagtggc ttaatcgcct gcgtgcgaat ccgaaaattc cattgattgt taccggttcc
     5221 gcggcagtgg cggtcatggt cgcactgatc ctgtgggcga aagcccccga ctaccgcaca
     5281 ttattcagca atctttccga tcaggatggt ggcgcaattg tcagccaact gacgcaaatg
     5341 aatattcctt accgcttcag cgaagccagc ggcgctattg aggttccggc agataaagtt
     5401 cacgaactgc gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg
     5461 ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg
     5521 ctggaaggcg agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta
     5581 catctggcaa tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg
     5641 gtgacggtaa atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta
     5701 catctggttt ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag
     5761 ggcgggcatc tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg
     5821 aaatatgcca gcgatgtcga aggccgtatt cagcagcgta ttgaagcgat cctgtcacct
     5881 attgttggta acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa
     5941 gaacaaacgg aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca
     6001 cgccagctta atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg
     6061 ttgtcgaatc aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa
     6121 aataaccgcc agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg
     6181 aatgaaacca gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc
     6241 gatgtgcaac gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa
     6301 ccattgcctc tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg
     6361 ggcttttctg aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt
     6421 gacgaaagcg gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg
     6481 gctgccggtc gctggttgct ggttgctggt actgctggtg gcgtggttgc tgtggcggaa
     6541 agcggtacgc ccgcagctaa cacgtcgcgc cgaggcggtg aagactgtgc agcaacaggc
     6601 gcaggcccgc gaggaagtgg aagatgcggt ggaagtccgt ctgagcaaag acgaacaact
     6661 ccaacaacgg cgcgctaacc aacgactggg ggcagaagtc atgagccagc gtatccgtga
     6721 aatgtctgat aacgatccgc gcgtggtggc gctggtcatt cgccagtgga taaataacga
     6781 tcatgagtaa cctgacaggc accgataaaa gcgtcatcct gctgatgacc attggcgaag
     6841 accgggcggc agaggtgttc aagcacctct cccagcgcga agtgcaaacc ctgagcgctg
     6901 caatggcgaa cgtcacgcag atctccaaca agcagctaac cgatgtgctg gcggagtttg
     6961 agcaagaagc tgaacagttt gctgcactga atatcaacgc caacgattat ctgcgttcgg
     7021 tattggtcaa agctctgggt gaagaacgtg ccgccagcct gctggaagat attctcgaaa
     7081 cccgcgatac cgccagcggt attgaaacgc tcaactttat ggagccgcag agcgccgccg
     7141 atctgattcg cgatgagcat ccgcaaatta tcgccaccat tctggtgcat ctgaggcgcg
     7201 cccaggccgc cgatattctg gcgttgttcg atgaacgtct gcgccacgac gtgatgttgc
     7261 gtatcgccac ctttggcggc gtgcagccag ccgcgctggc cgaactgacc gaagtactga
     7321 atggcttgct cgacggtcag aatctcaagc gcagcaaaat gggcggcgtg agaacggcag
     7381 ccgaaattat caacctgatg aaaacgcagc aggaagaagc cgttattacc gccgtgcgtg
     7441 aattcgacgg cgagctggcg cagaaaatca tcgacgagat gttcctgttc gagaatctgg
     7501 tggatgtcga tgatcgcagc attcagcgtc tgttgcagga agtggattcc gaatcgctgc
     7561 tgatcgcgct gaaaggggcc gagcagccac tgcgcgagaa attcctgcgc aatatgtcgc
     7621 agcgtgccgc cgatattctg cgcgacgatc tcgccaagcg tggtccggtg cgtctgtcgc
     7681 aggtcgaaaa cgaacagaaa gcgattctgc tgattgtgcg ccgtcttgcc gaaactggcg
     7741 agatggtgat tggcagcggc gaggatacct atgtctgata atctgccgtg gaaaacctgg
     7801 acgccggacg atctcgcgcc accaccggca gagtttgtgc caatggtcga gtcggaagaa
     7861 accattattg aagaggccga acccagcctt gagcagcaac tggcgcaact gcaaatgcag
     7921 gcccacgagc agggttatca ggcggggatt gccgaaggtc gccagcaagg tcacgaacag
     7981 ggctatcagg aaggactggc ccaggggctg gaacaaggtc tggcagaggc gaaggctcaa
     8041 caagcgccaa ttcatgcccg gatgcagcaa ctggtcagcg aatttcaaac tacccttgat
     8101 gcgcttgata gtgtgatagc ttcgcgcctg atgcagatgg cgctggaggc agcacgtcag
     8161 gtgatcggtc agacaccaac ggtggataac tcggcactga tcaaacagat ccaacagttg
     8221 ttgcagcaag aaccgttatt cagcgggaaa ccacagctgc gcgtacaccc ggatgatctg
     8281 caacgtgtgg atgatatgct cggcgcaact ttaagtttgc atggctggcg cttgcggggc
     8341 gatcccaccc tccaccctgg cggatgtaaa gtctccgccg atgaaggcga tctcgacgcc
     8401 agtgtcgcca ctcgctggca agaactctgc cgtctggcag caccaggagt ggtgtaatga
     8461 ccacgcgcct gactcgctgg ctaaccacgc tggataactt tgaagccaaa atggcgcagt
     8521 tgcctgcggt acgtcgctac gggcgattaa cccgcgctac cgggctggtg ctggaagcca
     8581 ccggattaca attgccgctc ggcgcaacct gtgtcattga gcgccagaac ggcagcgaaa
     8641 cgcacgaagt agaaagcgaa gtcgttggct ttaacggtca acggctgttt ttaatgccgc
     8701 tggaggaagt ggaaggtgtc ctgcccggcg cgcgtgtata tgccaaaaac atttcggcag
     8761 aagggctgca aagcggcaag cagttgccgc tcggtccggc gttattaggt cgtgttctgg
     8821 acggtagcgg taaaccgctc gatggcctgc ccttccccga tacgacggaa accggtgcgc
     8881 tgattacccc gccatttaac ccgttgcaac gtacaccgat tgaacatgtg ctggacaccg
     8941 gcgtgcgccc aatcaatgcc ctgcttaccg tcggacgcgg gcagcgtatg gggctgtttg
     9001 ccgggtcggg cgttggtaaa agtgtgctgc tggggatgat ggcacgctac acccgcgccg
     9061 atgtcattgt cgtgggtttg attggtgaac gtgggcgcga agtaaaagat tttattgaga
     9121 atatcctcgg tgccgaagga cgtgcacgct cagtggtgat tgccgctccg gcggatgttt
     9181 ctccgctcct gcgaatgcaa ggtgccgcct atgccacgcg cattgccgaa gattttcgcg
     9241 atcgtggtca gcatgtgttg ctgattatgg actccctcac ccgctacgcg atggcccagc
     9301 gtgaaattgc gctggcgatt ggcgaaccac ctgccactaa aggttatcca ccgtcggtgt
     9361 ttgccaaatt accggcactg gtcgagcgtg ccggtaatgg cattagcggc ggcggctcga
     9421 ttaccgcgtt ttataccgtg ctcaccgaag gcgatgacca gcaggacccc attgctgact
     9481 ccgcgcgggc aatcctcgac ggtcacattg tgctgtctcg ccgactggcg gaagccgggc
     9541 actatccggc tatcgatatt gaagcgtcga tcagtcgcgc aatgacggcg ttgatcagtg
     9601 agcaacatta cgcgcgagtg cgcaccttca aacagctgtt gtcgagtttt cagcgtaacc
     9661 gcgatctggt tagcgtcggc gcgtatgcca aaggcagcga tccgatgctc gataaagcca
     9721 tcgccctgtg gccgcagctg gagggctatt tgcaacaagg catttttgaa cgcgcggact
     9781 gggaagcgtc tctccagggg ctggagcgta ttttcccgac agtgtcataa cccaggagat
     9841 aacggcagat ggcggaagaa cagctcaaaa tgttgatcga ttatcagaat gaatatcgca
     9901 ataacctcaa tagcgatatg agtgccggga tgaccagcaa ccgctggatc aactatcagc
     9961 agtttatcca gacgctggaa aaagccatta ctcagcatcg ccagcaactt aatcagtgga
    10021 cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggcct
    10081 ggcagacact gcaggaacgg caatccacgg cggcactgct tgcagaaaac cgcctcgatc
    10141 agaaaaagat ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa tgattcgctt
    10201 agcgcccttg attaccgccg acgttgacac caccacattg cctggcggca aagccagcga
    10261 tgctgcacaa gattttctca cgttgttgag cgaagcatta gcaggcgaga caacaactga
    10321 caaagcggcc ccccagttgc tggtagcaac agataagccc acgacaaaag gcgagccgct
    10381 ggtcagcgat attcttgccg atgcgcaaca agcggattta ctgatccctg tggatgaaac
    10441 acttcctgtc atcaacgacg aacaatccac atcaacaccg ttaaccaccg ctcagacgat
    10501 gacgttggct gcggtggctg acaaaaatac gacaaaagac gaaaaagcgg atgatcttaa
    10561 tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac
    10621 gcccaaagtg actgatgcac cgtcaaccgt gttaccggca gagaaaccaa cgctcttcac
    10681 aaaactgact tctgcgcaac tcactacagc acagcctgat gacgcccccg gcacaccagc
    10741 tcagccatta acaccgctgg tagcagaagc ccagagtaaa gcggaagtca tcagcacgcc
    10801 ttcgccggtg accgctgacg ccagcccgct aatcactcca caccagacac agccactgcc
    10861 caccgtcgcc gcgcctgttt tgagtgcacc gctgggttct cacgaatggc aacaatcatt
    10921 gagccagcat atttcgctgt tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca
    10981 cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg gatgataacc aggcgcagat
    11041 ccagatgatt tcaccgcatc agcacgtacg cgccgccctg gaagcagcgc tgccggtact
    11101 gcgtacgcag ctggccgaaa gtggcattca gttagggcaa agcaacatta gtggcgaaag
    11161 ctttagtggt cagcagcagg ccgcttccca gcaacagcaa agccaacgca cagcaaacca
    11221 tgaacctctg gcgggggaag acgacgatac gcttccggtt cccgtctctt tacaagggcg
    11281 tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt cagaggtagc accgtaatcc
    11341 gcgtcttttc cccgctttgt tgcgctcaag acgcaggata attagccgat aagcagtagc
    11401 gacacaggaa gaccgcaaca catgactgat tacgcgataa gcaagaaaag caagcgatcg
    11461 ctttggatcc cgattctggt attcattacc ctcgcggcct gtgccagcgc aggttacagc
    11521 tactggcatt cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt cgtgccctcc
    11581 ccggtcttct atgcgctgga tacctttacg gtcaatttgg gcgatgcgga tcgcgtgctt
    11641 tatatcggca taaccctgcg actgaaagat gaagcaaccc gctcgcggct gagtgagtat
    11701 ttgccggaag tccgtagtcg cttgctgtta ctgttttcac gtcaggatgc tgccgtactg
    11761 gcgacagaag aaggcaagaa aaacctgatt gccgagatta aaaccacact ttccaccccg
    11821 cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc ttttattctg
    11881 cgataacgac atgggcgata gtattctttc tcaagctgaa attgatgcgc tgttgaatgg
    11941 tgacagcgaa gtcaaagacg aaccgacagc cagtattagc ggcgaaagtg acattcgtcc
    12001 gtacgatccg aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat
    12061 taatgagcgc tttgcccgcc attttcgtat ggggctgttc aacctgctgc gccgtagccc
    12121 ggacattacc gtcggggcca tccgcattca gccgtaccat gaatttgccc gcaacctgcc
    12181 ggtgccgacc aacctgaacc taatccacct gaaaccgcta cgcggcacag ggctggtagt
    12241 gttctcgccg agtctggtgt ttatcgccgt ggataacctg tttggcggcg atggacgctt
    12301 cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa cagcgcgtca tcaaccgtat
    12361 gttgaagctg gcgctggaag gctatagcga cgcctggaaa gcgattaatc cgctggaagt
    12421 tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat atcaccacct cgccgaacga
    12481 cattgtggtt aacacgccgt tccatgtgga gattggcaac ctgaccggcg aatttaatat
    12541 ctgcctgcca ttcagcatga tcgagccgct acgggaattg ttggttaacc cgccgctgga
    12601 aaactcgcgt aatgaagatc agaactggcg cgataacctg gtgcgccagg tgcagcattc
    12661 acagctggaa ctggtggcca actttgccga tatctcgcta cgcctgtcgc agattttaaa
    12721 actgaaaccc ggcgacgtcc tgccgataga aaaacccgat cgcatcatcg cccatgttga
    12781 cggcgtcccg gttctgacca gtcagtacgg tactctcaac ggtcagtatg cgttacggat
    12841 agaacatttg attaacccga ttttaaattc tctgaacgag gaacagccca aatgagtgac
    12901 atgaataatc cggccgatga caacaacggc gcaatggacg atctgtgggc tgaagcgttg
    12961 agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg cggtgttcca gcaatttggc
    13021 ggtggtgatg tcagcggaac gttgcaggat atcgacctga ttatggatat tccggtcaag
    13081 ctgaccgtcg agctgggccg tacgcggatg accatcaaag agctgttgcg tctgacgcaa
    13141 gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc tggatattct gatcaacggt
    13201 tatttaatcg cccagggcga agtggtggtt gttgccgata aatatggcgt gcggatcacc
    13261 gatatcatta ccccgtccga gcggatgcgc cgcctgagcc gttagtgatg aataaccatg
    13321 ctactgtgca atcttccgcg ccggtttctg ctgagccact gctgcaggtg agcggcgcac
    13381 tcatcgccat tattgccctg atcctcgctg ctgcctggct ggtaaaacgg ctgggatttg
    13441 cccctaaacg cactggcgtt aacggtctga aaattagcgc cagtgcttcg ctgggcgcgc
    13501 gtgaacgggt cgtggtggtc gatgtggaag atgcacggct ggtgctcggc gttaccgcag
    13561 ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc aacggaagag ataccgcaga
    13621 ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg cgggagatcc tgatgcgtcg
    13681 tttgttttct gtcgcaccgg tccttctctg gctgattacg cccctcgcct tcgcgcaact
    13741 gccgggtatc accagccagc cgctacctgg cgggtatcac cagccagccg ctacctggcg
    13801 gtggacaaag ctggtcgctc ccggtgcaga cgctggtgtt catcacctcg ttgacgttca
    13861 ttccagcgat tttactgatg atgaccagtt tcacccgcat cattattgtt tttggtttat
    13921 tgcgtaacgc gctgggaacg ccctccgcgc cacctaacca ggtattgctg gggctggcac
    13981 tgtttttgac cttttttatt atgtcgccag tgatcgacaa aatttatgtc gatgcgtacc
    14041 agccattcag cgaagagaaa atctcaatgc aggaggcgct ggaaaaaggg gcgcagccgc
    14101 tgcgtgagtt tatgctgcgt cagacccgcg aggcagattt agggttgttt gccagactgg
    14161 cgaataccgg cccgttgcag gggcctgaag ctgtgccgat gcgcattttg ctcccggcct
    14221 acgtgaccag cgagttgaaa accgcatttc agataggctt cacgattttc atcccttttt
    14281 tgattatcga cctggtgatt gccagcgtgt tgatggcatt ggggatgatg atggttcccc
    14341 ccgccaccat tgctctgccc tttaaactga tgctgtttgt actggtggat ggctggcaat
    14401 tgctggtcgg ttcgctggcg cagagctttt acagctagag aggcaaaatg acacctgaat
    14461 cggtcatgat gatgggaact gaagcgatga aagtcgcgct ggcactggca gccccgctgt
    14521 tgctggtagc gctggtcacg ggccttatca tcagtatttt gcaggcagcc acgcagatta
    14581 acgaaatgac gctgtcgttt attccgaaaa tcatcgccgt atttatcgcc atcattattg
    14641 ccggaccgtg gatgctcaat ctgttgctgg attacgtccg caccttgttc actaacctgc
    14701 cgtatatcat cgggtagccc gtacaatgat gcaggagaca agcgatcaat ggctttcctg
    14761 gttaagcctg tacttctggc cgttactgcg cgtgctggcg ctcatttcta ccgcgccaat
    14821 tctgagcgaa cgcagcgtgc cgaaacgggt aaaactgggt ctggcaatga tgatcacgtt
    14881 cgccattgcc ccatcattac ctgccaacga tgttcctgtt ttttcgttct ttgctctgtg
    14941 gctggccgta cagcagatcc tgatcggcat tgcgcttggt tttaccatgc aatttgcctt
    15001 tgccgctgtg cgaaccgctg gcgaaattat cggtctgcaa atggggctgt catttgcgac
    15061 atttgtcgat cctggcagcc atctcaatat gcccgtttta gcgcgcatca tggatatgct
    15121 ggcattactg ctgttcctga catttaacgg tcatttatgg ttgatttcac tgctggtcga
    15181 tacctttcac accctgccga ttggtagcga accgttgaac agcaatgcgt ttctggcacc
    15241 caccaaagca gggagtttga ttttccttaa cgggctgatg ctggcgttac cgctcattac
    15301 tctgttgctg accctgaatc tggcattagg tttactgaat cgtatggccc cgcaattatc
    15361 catttttgtt attggatttc cattaactct gactgtcggc atctctttaa tggcggcatt
    15421 aatgccgtta attgcgcctt tttgcgaaca tttattcagt gaaattttta atttgctggc
    15481 tgatattatt agtgaattac cattaatata attccgtaac gtttatcatg ctaccctaag
    15541 gattatccga aaaataatat ctgcgaacat cttccaggat actcctgtag cgaaatattt
    15601 gttttaagct cactcacata tcgcaacatt tactttaaga caattccagg caaattatac
    15661 aacactttac gggatagtaa gtccgcctga aaaattgcga gagtggcgca ttaggtgacc
    15721 catgttgttc cgtttagtca tgatgaaata ttcaggtaag gggaattatc gttacgcatt
    15781 gagtgagggt atgccatgtc aacgattatt atggatttat gtagttacac ccgactaggt
    15841 ttaaccgggt atctgttgag tagaggggtt aaaaaaagag aaatcaacga cattgaaacc
    15901 gttgatgacc ttgccatagc ttgtgattca cagcgtcctt cagtggtgtt tattaatgag
    15961 gactgtttca tccacgatgc ttctaacagt cagcgcatca agcacatcat taatcaacat
    16021 cccaatacgt tatttatcgt ttttatggca attgccaatg ttcattttga tgaatatcta
    16081 ttggtcagaa aaaatttatt gatcagttct aaatcgatta aaccggaatc tctcgacgat
    16141 atccttggcg atattctgaa aaaagagaca acgataacct cgtttttaaa tatgccgacg
    16201 ttatcattga gccgaaccga atcgagtatg ttgcgaatgt ggatggcagg tcagggaacc
    16261 attcaaatct ccgaccaaat gaatatcaaa gccaagaccg tttcatcgca taaaggtaat
    16321 attaaacgta agatcaaaac gcataataaa caggttatct accatgtcgt ccgactgacg
    16381 gataatgtga ctaatggtat ttttgtcaac atgcgctaac acattctgac tggtggtttc
    16441 ccaccagtca ggctgaataa gattactctg ctttctcaac aaagataccg tcctgatgcc
    16501 ctgcttcatt aaagaaccag atgccgagcg ggtagtcttc cagcgcaacc aggtacattg
    16561 tgccttcact aaactcctca actgccagta ccactccagg acgacgcgga ccgccatccg
    16621 ttttgactgt tacccgatca ttcaccttca tcgttttcct cctgtggctt tgtgccagtg
    16681 tagaacaatt tcgtttttct ggcagcgcca agcgcgcgcg agtgctgatt ttctcgacgg
    16741 tctatactta agagatgcca ggcggactta acgactggcg gcaacaacag agtaacggtt
    16801 gcgaggaaag atgatgaaaa ccgcaaaagt gtacagcgat accgcaaaac gtgaggtcag
    16861 cgtcgatgtc gatgccctgc tggcggcgat caatgaaatt agcgaaagcg aagttcatcg
    16921 cagccagaac gattctgaac acgttagcgt cgatggacgt gaatatcata catggcgtga
    16981 attggcggat gccttcgaac tggatattca tgacttcagc gtctctgaag tgaatcgttg
    17041 aatgcacaat aaaaaaatcc cgaccctgag ggtgtcggga tgaaacttgc ttaagcaaga
    17101 agcacttaaa aaattcgtta caccaggaaa tctgatgtgt tcatcacctt atctgcaatt
    17161 tttttcgctg acaagaaaat attcgcgcta tgaatgatta acgctactta tgaatagaaa
    17221 tgtgacgcgc gtcacccctt ggtggctctg gtgtcaacgg gatcgctccc gaaaaaatca
    17281 tctttgatta tttgtgctgt tttgagaatc atcctggtaa ggggagtact gcgcgaccat
    17341 gttttcaatt caacaaccac tactggtttt tagcgatctt gatggcaccc tgctggacag
    17401 tcatagttat gactggcaac cggcagcccc ctggctcagc cgtttacacg aagccaatat
    17461 tcccgtcatt ctctgtagca gtaaaacttt agcggaaatg ctgtacttgc aaaaaatgtt
    17521 ggggctacaa ggcttaccgc tgattgcaga gaatggcgca gtgatccagc ttgctgaaca
    17581 atggcaggat atagacggtt ttccacgcat catctcaggt attagccatg gcgaaatctg
    17641 ccaggtttta aatacgctac gcgagaaaga acattttaaa ttcacgactt ttgatgatgt
    17701 cgacgatgca accattgccg aatggacggg attaagccgt agctaggcgg cgctgacgca
    17761 gctgcatgag gcgtcggtaa cgctaatctg gcgcgacagt gacgagcgta tggcacaatt
    17821 tatcgctcgt ctgaacgaac tgggcttgca gtttatgcag ggtgcgcgct tctggcacgt
    17881 actggatgcc tctgccggaa aagatcaggc tgccaactgg attatcgcga cctatcaaca
    17941 attgtcaggc agacgcccaa ccacacttgg cctgggcgat gggccaaacg atgcgccttt
    18001 actggaggta atggattacg cggtgattgt gaaagggcta aaccgtgaag gggtacatct
    18061 gcatgatgag gatccggccc gcgtctggcg aacgcagcgt gaaggaccgg aaggctggcg
    18121 tgaagggctg gaccattttt tctccgcccg ttaagcgtta tcgctcgcga atacccgatt
    18181 gcgcccggcc tgttttgcga ggtaaagccg acggtcggcc aatgactgga gttgttcaaa
    18241 atcataatca ccggtttcct cgctgctact tacccccagc gaggcactga tgcgtatcgt
    18301 tgtactcttg gcgatcagca tctctttttc atttaacttc aggcgaatac gttctgcgac
    18361 ctccgcagcc tgcgtcagat tcgcgcctgg cagaatcaca caaaactcct caccaccgac
    18421 ccgcccggca acgtcctgcg cacgcaagga actgctaatt aatccggcag catgagaaag
    18481 aacacggtcg cccgcctgat gaccaaagcg gtcattaatc gctttaaaat ggtcaagatc
    18541 gacctgaatg acagaaaaag gatgttggtg cgtctgacac aatttagcga gcggacgggc
    18601 tttttcgaac agtgcgccac ggttatataa acgcgttaag gtgtcgtgcc acgcctgcca
    18661 ctgcaacgag ctttgcagaa catacatgtt gctgaccatc cggcgaatca cataccagga
    18721 gaggagtaac atggtggtaa agagcgccca cagcagggtt aatgcaatgc tgatactgcc
    18781 gaaatcgccg cgcacgcctt cgcttagcgt atggacgcgc gccagcacac cgtcgaaatg
    18841 gtccagacgt tcccagctaa catagcgact gtccatacga atgccgcccc gcgtgtcatg
    18901 ttccattgcc tgcgccagca aggccagttc acgaggatca aaaatattcc ctgttggatg
    18961 gtcaggattg gaagaggtca aaaatctcag cttactgtca tagagctgat actcaccatc
    19021 gaggttttta tcgatggcgt ttcttaaaaa ttgctgcata gtacgcacgg gaatactcat
    19081 ccccagcacg ccataccagt agttattact gtctaccgga acactgacgg taacctgcgg
    19141 ttcagtattg ctggcgtgtt ccggttgcga agtgaaccag cgtaccgcgc ggtgacgatt
    19201 ttctcgttgc gaatggccga taaaccaggg ttgggtgaca tagccgtaat aacgcgttgg
    19261 tacattgcgc gtaaacaagg tcggctgcgt cgaaacgtaa aatccagcac gcgagacata
    19321 catcgcctgt tcaaccatcg acgaggagtt gtgcgccagt cgcagcaagt aaccaacttc
    19381 cagtgcagcg gtaatttcat tgtcgaggct ttcattttcg cgagacagga aattcccctc
    19441 gctgactaat gcatccgata cgccattgac tggcagggtg cgccgtcggt tgagttcgat
    19501 ttgccaggcg tgctcgtcgc gatgctgttc gaactcggtt acggcattac gcagagaggt
    19561 gaaatccagt ggcgccacga gagcttcgcg catgccatta cgcaaaaaga tcagtttgtc
    19621 gacgttatac tgcaaatgct tatcgagcgc gttggctacg ttttccagat gattacgcta
    19681 gctggagata taggcatctt ccagcaccac cacttcacgc caggtgagca aggtggaaaa
    19741 aagcaatacc acgataaagc agagattaac gacatgaccg ggccccaggc ggcgtgcgag
    19801 ttttttcaac cagctctggt tttccatttt tgtctcgtgc tgcaccctga cgactcccaa
    19861 cctgcttttt tatgattctg gcataacccg gcaatagcgt accgcaaaac agcgataatt
    19921 ttataatggt ggcatgataa gacgcgacta tcattcattg caggcattgg gacaccaggc
    19981 cggatgtgat ccggcctggt gttaatgata ccctttcaaa cttcttccgc agaacgactc
    20041 ccctcccccg gaacaagatc ggtttcatga aaagcttccc gcttgacccc gtaaccgtca
    20101 taccaacgac actcaaccat accgctggag tatccagtga caatcatccg cgggccgccc
    20161 tctttaaccg taacttcctc actaaccata aagctcatac tcgcctcctt ttttcgagtg
    20221 aaacttgttc accttagttg aagatggcga attttgcata atagccagtg cgagtattaa
    20281 tgtgcctgac cgcgcatttt cgctactgct ttcactccca gcaccacgat gccgccgata
    20341 ataaatccaa gaatcagatt taaaacagtc ggtaatatca tcgccaccac tgcactttgc
    20401 tgcccggcga tatgttcaat agcgtgatgc agcggcgcaa taccatgtac cacaatcccg
    20461 ccgccgacga ggaacatcgc cagcgtgccg acaatcgata atgctttcat cagccaaggc
    20521 gcgataatca ataacccttt acctaatgcc tgcatcagcg cgctagattt ttccgccagc
    20581 caatacccta ggtcatcaat cttaacgata acccctacca gaccgtagac gcccacggtc
    20641 actaccagcg cgatgcctga aagcaccagc acctgattaa gcaacggcgc ctctgccaca
    20701 atccccagcg tgatggcgac gatttccgca gacaaaataa aatcggtacg aatcgcccct
    20761 ttaattttgt ccttttcaaa cttcagcgga tcctgcgccg ccagcttctc cagacgctgc
    20821 tggctctgcg ctggatcttc tttatgttta cgcgcctcca gcatatgcag cactttctct
    20881 accccttcaa agcagagaaa cgcgccacca atcatcaaca gtggcgtaat cgcccacggg
    20941 ataaacgcac tgatgatcag cgccagcggc accagaatca ctttattgat cagcgatcct
    21001 ttcgccacgc cccagaccac gggaagttcc cggttggccc gcacgcctga aacttgttgc
    21061 gcattgagcg ataaatcatc ccctaataca ccggcggttt tcttcgccgc cagtttgccc
    21121 atcacagaga tatcgtccag cagtgtggcg atatcatcga gcaacgtcag taaactactc
    21181 ccggccaaaa gcattccctc tcatttttat tgttgaaggg tgtaagtatg aagcaaaaac
    21241 ggcagctctg atacttgctt cacaggtcaa catttattta acaatcaagt gcaattatat
    21301 ctctcgccag cagaatgatt ttcacgttta ctatgaacca cctttttatt tccgccgtga
    21361 gggattatgc gtttccgcca gttgttaccg ctttttggcg cgctgtttgc gttgtatatc
    21421 atttggggct caacctattt tgtcattcgg attggcgtgg gaagctggcc tccgttaatg
    21481 atggcgggcg ttcgattcca ggcagccggt attttattgc tggcattttt gctactgcgc
    21541 ggacacaaac ttcccccgct acgtccgctg ctcaatgccg cgctgattgg cctgttattg
    21601 ctggctgtcg gtaatggcat ggtgacggtt gccgaacatc aaaatgttcc ttccggcatc
    21661 gccgccgtag tgattgcaac cgtgcccctc tttaccctgt gcttcagtcg cctgtttggc
    21721 attaaaacgc gcaaactgga atgggtgggt attgccattg ggcttgccgg aatcatcatg
    21781 ctcaatagcg gtggaaattt aagcggcaat ccgtggggcg cgattctgat tttaatcggc
    21841 tcgattagct gggcgtttgg ctcagtttat ggctcgcgca ttaccttacc tgtagggatg
    21901 atggcgggtg cgattgagat gctggcggca ggcgtggtgt taatgatcgc atcgatgatt
    21961 gcgggtgaaa aactgacggc gctcccttcc ctgtcaggct tccttgcggt cggctatctg
    22021 gcgctgtttg gttcgattat cgccattaac gcttatatgt atttaatccg taatgtcagt
    22081 ccggctctcg ccaccagcta cgcctacgtt aacccggtgg tcgcggtctt gctgggtacg
    22141 ggactgggtg gcgaaacgct gtcgaagatt gaatggctgg cgctcggcgt aattgttttc
    22201 gcggtggtac tggtcacgtt gggaaaatat ctcttcccgg ccaaacccgt tgtcgcgcca
    22261 gttattcagg atgcatcaag cgagtaaatg aatcccctgc gtgtcgatct gcgcgctggc
    22321 accttcgccg cagatccact cttccagacg ctcggtaagc gccgcatccg tcagcttctc
    22381 gcgcctacgt aacgcacact cccagacaat cagtacgcgc cagccgagct cctgcaagcg
    22441 actgatatcg cggcgatcgc gttcaacatt tttacctatc ttctccagcc agaactcagt
    22501 tcgagtcgca ggcactttaa acagatagca gtgatgatga tgccagaagc agccgtgggt
    22561 aaatatcacg cagcgatatt catcaacgac aaaatccggg cgtccgggca gactggcgtc
    22621 ctgaacgcga aacgcaaggc cctgcccggt taacaaactg gcgaggcgct tctcaatcgc
    22681 cgtatcacgc gtggcaatcg cgcgcatatt tttgctgcgg gtggccttat cgtgaacgtc
    22741 gaccatgttg tgcctcttgc tgacgcaacg ccaccgcctg tttgattttt ggctcaagca
    22801 gttttgccac cgcggcaaag accggcacga ccaccgagtt gccgaactgg cgataggcct
    22861 gagtgtccga aaccggaatg cggaatttcg cttctcccgg cgcttcaaaa cccattaagc
    22921 gcgcgcattc acgaggcgtt aaccgacgtg gacgatgttg ctgattcagc ggatcgtcaa
    22981 agtctttctc acccgtggcc atatcccaac cgcgatcgat taaaatttcc gcgccatctt
    23041 tgtaataacg agcagacagc gtacgggtga cgctttgcgg attgttcgga taaaccattc
    23101 cataaccgaa gccgttaccg cgcgcctgat gttttttcgc atatcgatag aggtacttcc
    23161 acagcaccgg cgtcaggata tatttcgcct caaccatcgg gtccagcagc tgcgccagcg
    23221 tcactcgctg tgcagggaaa cattcgctga tatcacgcag agtaaaatcg gctttcagat
    23281 tcagatcgcg acgaaaaccc accagcacga tgcgttcacg gtgctgcggc aggaaatgtt
    23341 taccatcgat gattttcgga tcgtccggcc cgttatcttc tgcatcggca acgtcatagc
    23401 ccagttcgtc cagcgtctgc atgatgatgc ggaacgtttt tcccttatcg tgactcttca
    23461 ggtttttgac gttttcgagc acaaacatcg ccggacgacg cgcgtcgata atgcgcacca
    23521 catcaaagaa cagcgtgccc tgggtatcgc aggcaaaacc gtgcgcccgc ccgagcgagt
    23581 tctttttcga tacgccagcc agcgaaaatg gctgacaagg gaaaccggcc agtaaaacat
    23641 cgtgttcagg aatgtgttga cgaatatgtt ccgccgccgc ctcatcactc acgccttctt
    23701 gatgactgag ggtgatgtcg cggatatctt cattaaaatg atgcgtcgcc gggtcgcaat
    23761 aatggttggc tttataagtg cgtaccgcat gtttgttcca ttcgctggta aacacgcact
    23821 gtccaccaat cgattcaaaa ccacgacgga tgccgccaat tccggcaaat agatcgataa
    23881 agcgaaacgc ataatgcgga tgatgttccg gtggtttggg taacaacgtt tgcagatggg
    23941 caaactcttt ctcacttaaa cggtgccatg ccgagtcatt cgccagcgca cgttttaaaa
    24001 ttgccgcgct ccagtgattc tcacccacac cattaagctg cgccaccagc attttaacat
    24061 cataaatttg cagcagtttc tccagcattg cctgtgcggc attcccggtg ctgtatgaat
    24121 cggttactga tatattttcc tgcatagatt taaccggcca tctgagatgg ccgacaggtt
    24181 aacataatta ctgccgttta gggccacgcg ctcagccagc gggtgaaaac gcatctatca
    24241 ccttgtgatc gataccttca ttctcccccg ccagttcggc accgagcttc gccatcaaac
    24301 agcgccaagc tcaatgcccc tgaaacggca aatacgcgcg ccaggccaat cgcccccagg
    24361 gcttccagcc gatccgcatc ctggacgatt ttagcttccg ttgttaaggg ggcgatttgc
    24421 gcgctgaaac tgtgagcggc aatggcatga caaacggcct caattttctc cgccggaaat
    24481 tgcacgaact cttcacggag caggcgacgc gtctcttctg ccgccaggat tgaagaacgc
    24541 tgccgttgcg gatgattttt cgccaggctc acgatatcgt gaaaataaca ggcggttaaa
    24601 atcaccagca tatcgacatc gtcatctgcc gccagtttct gtgccgtagc ccagacacgg
    24661 cgaaaatgac acaggtcatg cgccgcgtcc tgatgttgat gatggttttt caaccagttc
    24721 tcgaactatg cctgccagtg ttgtaactcc atacgcgctc cgttgccaaa gagtctcaac
    24781 cttagcagtt tttattcata gcgtctgcaa cgtgcgggac gaggtcgggt gtaccatgca
    24841 atgccgccta acaaggcacc aaccagggcc ataaacagaa atccgaccaa tgctcctaac
    24901 cacttccccg cggtagatcc taaatcactt tcaaatcccg acacgggggc atcggggatc
    24961 cggctcatca cccagatgta tcgcgccatt gcccagacga aatagccaca ccatgtatag
    25021 aaagccc
//
LOCUS       DAJTNP010000056        24273 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTNP010000056 DAJTNP010000000
VERSION     DAJTNP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1..386)
                     /locus_tag="OO160_002453"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO160_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO160_002454"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO160_002454"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5524533.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO160_002455"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO160_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5524534.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO160_002456"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO160_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5524535.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO160_002457"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO160_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5524536.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO160_002458"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO160_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5524537.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO160_002459"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO160_002459"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5524538.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO160_002460"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO160_002460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5524539.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO160_002461"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO160_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5524540.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO160_002462"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO160_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5524541.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO160_002463"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO160_002463"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5524542.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO160_002464"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO160_002464"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5524543.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO160_002465"
     CDS             10748..11212
                     /locus_tag="OO160_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5524544.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO160_002466"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO160_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5524545.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO160_002467"
     CDS             12236..13672
                     /locus_tag="OO160_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5524546.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO160_002468"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO160_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5524547.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO160_002469"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO160_002469"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5524548.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO160_002470"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO160_002470"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5524549.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO160_002471"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO160_002471"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5524550.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO160_002472"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO160_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5524551.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO160_002473"
     CDS             complement(20505..20798)
                     /locus_tag="OO160_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5524552.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO160_002474"
     CDS             complement(20795..22084)
                     /locus_tag="OO160_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5524553.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO160_002475"
     CDS             complement(22140..23078)
                     /locus_tag="OO160_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5524554.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO160_002476"
     CDS             complement(23098..23862)
                     /locus_tag="OO160_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5524555.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5998 a         5872 c         6384 g         6019 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggggcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    24001 gcgattgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    24061 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    24121 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    24181 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24241 acggctttca cgttttgcat ttagcgatga tcc
//
LOCUS       DAJTNP010000057        24182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTNP010000057 DAJTNP010000000
VERSION     DAJTNP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(22..594)
                     /locus_tag="OO160_002477"
     CDS             complement(22..594)
                     /locus_tag="OO160_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5524556.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO160_002478"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO160_002478"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5524557.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO160_002479"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO160_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5524558.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO160_002480"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO160_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5524559.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO160_002481"
     CDS             complement(3556..5337)
                     /locus_tag="OO160_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5524560.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO160_002482"
     CDS             complement(5330..7102)
                     /locus_tag="OO160_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5524561.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO160_002483"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO160_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5524562.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO160_002484"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO160_002484"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5524563.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO160_002485"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO160_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO160_002486"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO160_002486"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5524564.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO160_002487"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO160_002487"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5524565.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO160_002488"
     CDS             complement(11658..12029)
                     /locus_tag="OO160_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5524566.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO160_002489"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO160_002489"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5524567.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO160_002490"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO160_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5524568.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO160_002491"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO160_002491"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5524569.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO160_002492"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO160_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5524570.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO160_002493"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO160_002493"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5524571.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO160_002494"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO160_002494"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5524572.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO160_002495"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO160_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5524573.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO160_002496"
     CDS             21800..22378
                     /locus_tag="OO160_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5524574.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO160_002497"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO160_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5524575.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6664 c         5827 g         6025 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 at
//
LOCUS       DAJTNP010000058        23718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTNP010000058 DAJTNP010000000
VERSION     DAJTNP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..812)
                     /gene="rluF"
                     /locus_tag="OO160_002498"
     CDS             complement(<1..812)
                     /gene="rluF"
                     /locus_tag="OO160_002498"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5524576.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGG"
     gene            1160..2107
                     /locus_tag="OO160_002499"
     CDS             1160..2107
                     /locus_tag="OO160_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5524577.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2177..2983
                     /locus_tag="OO160_002500"
     CDS             2177..2983
                     /locus_tag="OO160_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5524578.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            2993..3400
                     /locus_tag="OO160_002501"
     CDS             2993..3400
                     /locus_tag="OO160_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5524579.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3400..3894
                     /locus_tag="OO160_002502"
     CDS             3400..3894
                     /locus_tag="OO160_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5524580.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            3960..4757
                     /locus_tag="OO160_002503"
     CDS             3960..4757
                     /locus_tag="OO160_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5524581.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4768..5556
                     /locus_tag="OO160_002504"
     CDS             4768..5556
                     /locus_tag="OO160_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5524582.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5608..6837
                     /gene="sorE"
                     /locus_tag="OO160_002505"
     CDS             5608..6837
                     /gene="sorE"
                     /locus_tag="OO160_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5524583.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7219..7908
                     /gene="pepE"
                     /locus_tag="OO160_002506"
     CDS             7219..7908
                     /gene="pepE"
                     /locus_tag="OO160_002506"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5524584.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7999..9630)
                     /locus_tag="OO160_002507"
     CDS             complement(7999..9630)
                     /locus_tag="OO160_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5524585.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9850..13533)
                     /gene="metH"
                     /locus_tag="OO160_002508"
     CDS             complement(9850..13533)
                     /gene="metH"
                     /locus_tag="OO160_002508"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5524586.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13733..14557
                     /gene="iclR"
                     /locus_tag="OO160_002509"
     CDS             13733..14557
                     /gene="iclR"
                     /locus_tag="OO160_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5524587.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14874..17058
                     /gene="espL4"
                     /locus_tag="OO160_002510"
                     /pseudo
     CDS             14874..17058
                     /gene="espL4"
                     /locus_tag="OO160_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17027..18764)
                     /gene="aceK"
                     /locus_tag="OO160_002511"
                     /pseudo
     CDS             complement(17027..18764)
                     /gene="aceK"
                     /locus_tag="OO160_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(18948..20252)
                     /gene="aceA"
                     /locus_tag="OO160_002512"
     CDS             complement(18948..20252)
                     /gene="aceA"
                     /locus_tag="OO160_002512"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5524588.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20282..21883)
                     /gene="aceB"
                     /locus_tag="OO160_002513"
                     /pseudo
     CDS             complement(20282..21883)
                     /gene="aceB"
                     /locus_tag="OO160_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22152..23081)
                     /gene="metA"
                     /locus_tag="OO160_002514"
     CDS             complement(22152..23081)
                     /gene="metA"
                     /locus_tag="OO160_002514"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5524589.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23238..23681
                     /locus_tag="OO160_002515"
     CDS             23238..23681
                     /locus_tag="OO160_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5524590.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5843 a         6046 c         6017 g         5812 t
ORIGIN      
        1 ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc cgctgttttc
       61 ggtttggcct tcgctttagg ttttgcctcg gaagaggaat tttcgatgag cttaaagagg
      121 tcgattaact catcgtcggt taaatcgcgc cactccccca gcggaatgcc gcttaagctg
      181 acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg ctcgcacatg
      241 cgccggatct gacggttcag cccctgcact agggtaatgc ggaagacaaa cggcgcttct
      301 tttttgactt tgcacttttt cgtcaccgtc ccgaggatcg gcacccccgc ccccatacca
      361 cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt ctcatgatcg
      421 ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa aatcagcccc
      481 tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt aacgaaatcg
      541 acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa taccgacggg cttgttcagg
      601 gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt tactttcaca
      661 atgtcgccgg gtttcacctg atcgccaatg gtggctcgct tgccattaag gaacacattg
      721 ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc gctgatgtat
      781 ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg cggaatatac
      841 cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa atgtgatctg
      901 ccacgccttt tatcacaaaa gtgtctccgg aagtacgatg caaatttgcc tggcaaaata
      961 aggataatca acatattacg aatcgctgca cacctgtgct gatgactact acgcagagaa
     1021 acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa tcgctttgaa
     1081 aggccctcat gatggggtta tatcagatca agcggtgaga aagcgtattt gcacaaatgt
     1141 gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg tgaagattgc
     1201 ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac tgggtattta
     1261 ccgcaccacc atcagccgct tgcttaaacg aggccgcgat cagggaatgg tcaccatcgc
     1321 catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc aaaagtttgg
     1381 cctgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc aactggcgat
     1441 gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg atattgtcgg
     1501 tttttcctgg ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcagg cggggcaatc
     1561 gcggcagtta atctgcgtac caattattgg cggaccgtcc ggtaaactcg aaagccgcta
     1621 tcatgtaaac acattaacct acagcgcggc agcgaagctg aaaggggaat cgcatctcgc
     1681 ggattttccg gctctgctgg ataacccatt aattcgtaat gggatcatgc agtctcagca
     1741 ctttaaaacc atctctgcct actgggataa tctggatatc gccctggtgg gaattggctc
     1801 accggccatt cgcgacggcg ctaactggca tgcgttttat ggtggtgaag agagtgacga
     1861 cctgaatgcc cgccaggttg ctggcgatat ttgctcgcgc ttttttgata ttcacggcgc
     1921 aatggttgaa acgaatatga gcgaaaaaac gctctctatc gaaatgaata aattaaagca
     1981 ggcgcgatat tccattggca ttgccatgag tgaagaaaaa tacagcggaa ttattggtgc
     2041 actgcgtgga aaatatatta attgtctggt aacgaatagc agcacagctg aactattact
     2101 gaaataaaaa tcttcccgat gtttaccgta ataaaaatta cggcgggtcc ctattatcta
     2161 aaatcaggag tctgttatgc aaacgtggtt aaatttgcag gataaaatca ttattgtcac
     2221 cggcggcgca tccggtattg gtctggcgat tgtagaggaa ttattagcac aaggcgcgaa
     2281 tgtacagatg gtcgatattc acggtggcga tggtcaatat gaaggtcata aaggctatca
     2341 gttttggccg accgatattt ctagcacaaa agaggtaaat catacggtag cggaaattat
     2401 ccagcgtttt ggtcgcatcg acggtctggt caataacgcc ggggtcaatt tcccgcgtct
     2461 gctggtcgat gagaaagcgc ctgccgggca gtatgaactc aacgaagctg cattcgaaaa
     2521 aatggtcaat atcaaccaga aaggtgtttt tctgatgtcg caggcggtgg cgcgacagat
     2581 ggtcaaacaa catgatggcg tgattgtgaa tgtttcctca gaaagtgggc tggaaggctc
     2641 agaaggacaa agctgttacg ccgctaccaa agccgcgctc aatagcttca cgcgctcctg
     2701 gtcgaaagag ctgggtaaac acggtatccg tgtggtcggt atcgcgcccg ggattctgga
     2761 aaaaaccggg ctacgcacgc cggaatatga agaggcgctg gcgtggacgc gcaatatcac
     2821 cgtcgagcag ctgcgtgaag gctataccaa aaacgccatt cctattgggc gcgccggaag
     2881 attagcagaa gtagctgatt ttgtttgtta tctgttgtct gaacgcgcca gctacatcac
     2941 cggagtaacc actaacattg cgggcggcaa aacgcgcggc taaggaggct ttatggtcaa
     3001 cgccattttt tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt
     3061 ttacggcaac gccaatgttg aagccgttgc atttgtgccc ggtgaaaacg ccggagacat
     3121 tgcggtaaag ctggaaaagt tagtaagcat tcacaatcaa gatgagtggc tgattgccgt
     3181 ggatttacag tgcggcagcc cgtggaatgc ggcagcaatg cttgcgatgc gcaatcctcg
     3241 tttgcgggta atcagcggcc tttctctgcc gctggcgctc gaactggttg ataaccagga
     3301 cagcatgaat gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt
     3361 tgtctggaaa caactggcaa cgacagagga ggatttctga tgaacattac gctcgcccgc
     3421 attgatgacc gtctgatcca cggccaggtc actaccgtct ggtcgaaagt ggctaacgcc
     3481 cagcgcatta ttatctgcaa tgacgaagtt tataacgatg aagtgcgccg gacattattg
     3541 cgtcaggctg cgccaccggg aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg
     3601 gtttatcaca acccgcaata tcaggacgaa accgtttttt atcttttcac tcgcccacag
     3661 gatgccttag cgatggttcg ccagggggta aaaataggca ccctgaatat tggcggcatg
     3721 gcctggcggc ccggtaaaaa acagttaacc aaagcggttt cattagatga tgatgatatt
     3781 aacgcatttc atgagttaaa taatcttggt gtcatactgg atttacgcgt cgtagcgtca
     3841 gatccctcaa tcaatattat cgacaaaata aacgaacagt taatcgcaaa ttaaataata
     3901 gcgcctgtgt acatatgctt taacaggcag ggatgacgac acttaaaagg tgacaaatca
     3961 tggaaataag taccctacaa atcattgcta tatttctttt ttcctgtatt gccggaatgg
     4021 gcagcgtgct ggatgaattt cagactcatc gcccgttaat tgcctgtacg gtgattggtt
     4081 taattctcgg tgatttaaaa accggaataa tactcggcgg tacgctggaa ttgatagctc
     4141 tctgctggat gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc agcataatct
     4201 ccgccattct ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc
     4261 ctgtggctgc ggcaggccag gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc
     4321 agcacgcggc ggataaagca gcagaagagg cgcggtttcg cactctcgat attctgcatg
     4381 tctccgcgct tggcgtgcag gcgctgcgcg tcgccattcc ggcactgatt gtctcactgt
     4441 tcgtcagcgc cgatatggtc agcaatatgt taaacgccat tcccgaattt gtgacccgag
     4501 gactacagat tgctggcggt tttatcgtgg tggtcggtta cgccatggtg ctacgtatga
     4561 tgggcgtgaa atatttgatg cctttctttt tcctcggttt cctcgcaggt ggctacctcg
     4621 atctcagcct gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc
     4681 taaatccaca gtggtgtaaa gctgaacctc aactccaggc caccgcctcc accgcccttg
     4741 accaacttga cgattaacgg agccatcatg gaacagagaa aaattacacg cagcgatctg
     4801 gtgagcatgt ttctgcgctc caacctgcaa caggcgtcct ttaactttga acgtattcac
     4861 gggctgggct tttgctacga catgatcccc gccatcaagc gactttaccc attaaaagag
     4921 gatcaggttg cggcgctcag gctacacctg gtgttcttca ataccacgcc agccgtatgt
     4981 ggcccggtca tcggcgcgga aattgatgac ggtaccatca acggcatcaa agtcggtctg
     5041 atgggaccat tggcaggagt tggcgatcca ctggtctggg gaacgctgcg cccgattacc
     5101 gccgcgctcg gcgcatctct ggcactttcg ggcaacattc tcggcccgct gctgttcttc
     5161 tttattttca acgcggtgcg tctggcgatg aagtggtatg gcctacagct cggctttcgc
     5221 aaaggggtga atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact caccgaaggc
     5281 gcgtcgattc tcggactgtt tgtgatgggc gtgctggtga ccaaatggac gtcaatcaac
     5341 gtaccgttgg tggtttcaca aacgcatgcc gccgatggct ccaccgtcac catgaccgtg
     5401 cagaacattc tcgaccaact ttgccctggt ttgctggcgc tcggtctgac gctactaatg
     5461 gttcgtctgc tcaacaaaaa aattaacccg gtatggctga ttttcgccct gtttggctta
     5521 gggattatcg gcaatgctct gggcttcctg tcctgattct tcgccccggc acgactgccg
     5581 aggccatcgc tcaacatgag gtggtttatg aaaacaacag ctctgcgtct ttatggtaaa
     5641 cgtgatttac gcctggaaac ctttgacctt cctgaaatgc aggaggatga aatcctcgca
     5701 acggtggtca ctgacagcct gtgcctctct tcctggaaag aggccaatct gggtgaaaac
     5761 cataaaaaag tacccgacga tgtgacgacc aacccaatca tcatcggcca cgaattttgc
     5821 ggcgatattc tggccgtggg taaaaagtgg cagcacaaat tccagccggg tcagcgttat
     5881 gtgattcagg ccaacctgca actccccgac cgcccggatt gccccggcta ctccttcccg
     5941 tgggtagacg gcgaagcgac gcatgtggtt attcccaacg aggtcatgga acaatattgc
     6001 ctgctggcat acgacggcga aacctatttt gaaggctcac tggttgaacc gctttcctgc
     6061 gtgattggcg cgttcaacgc caactatcat cttcaggaag gtagttataa ccacacgatg
     6121 gggattcgcc cgcaagggca cacgctgatc ctcggcggca ccggaccaat gggactgttg
     6181 gcgattgatt atgcgctgca tggaccagtt aacccgtcgc tgctcgtcat taccgatacc
     6241 gacaacgaca aattgagtta tgcgcgcaag cactatccat cagaaccgca aacactgatt
     6301 cattatctca atgccaccga tgcagcattt gatacgttaa tggcgctgag tggcggtcac
     6361 ggcttcgatg atattttcgt ctttgtgcct aatgaaggac tggtgactct cgcctcttcc
     6421 ttgctggcga cagatggttg cctgaatttc ttcgccggac cgcaggataa acatttcagc
     6481 gcgccaatta atttctacga tgtgcattat gcatttaccc actacgtggg cacgtcaggc
     6541 ggcaataccg acgacatgcg cgcggcggtc aaactgatcg aagagaaaaa agtgcaggcc
     6601 gcaaaagtgg taacacatat tcttgggctg aatgccgcgg gcgaaaccac gcttgaattg
     6661 cctgccgtcg gcggcggcaa aaagctggtg tataccggga aatacctgcc gctgacgtca
     6721 ctcacgcaga ttcaggatca agcactggcg gcgattctgg cgcgtcatca ggggatctgg
     6781 tccggtgagg tggagcaata cctgctcgcc catgcagagg caatttccca tgattaatcg
     6841 cgctacttaa ctctgcatgt cgctggctgg tcgccccagc aacttcgcga ccggatgtgc
     6901 ggctgattgc tgaagcagca caatttgcac gagaaggata aaaaatatca gcgccttaag
     6961 gggcgctcaa attggtggca aagtacacat tgtttatgcc ggatgcggaa tgaagcctta
     7021 ttcggtctac ataattttga aaatccaata tattgcagtt atctggtagg ccaggtaagc
     7081 gtagcgcatc aggcaatgct gtgtttgtca tcagtctcag cgcccggtgc aatacgtgac
     7141 atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcatgcctt ccgtgatttc
     7201 ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct gccgggtaaa
     7261 gcctggctgg aacatgcact gccgctaatt gctgaacagt tgcagggtcg ccgctcagcg
     7321 gtgtttatcc ctttcgctgg cgtaacgcag acctgggatg attacacaga gaaaacggct
     7381 gcggttctcg ctccgctggg tgtttctgtc accggtattc atagcgttgt cgatcccgtt
     7441 gccgcgattg aaaatgcgga aattgtgatt gtcggcggcg gtaatacttt ccagttgctg
     7501 aaacagtgcc gcgagcgcgg gctgctggct ccaattactg acgtggttaa acgtggcgcg
     7561 ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caaccattcg taccaccaac
     7621 gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc gttgcaaatc
     7681 aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg tgagcagcgt
     7741 attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctgcc ggaaggtaac
     7801 tggatcacag tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac ttatgtgttt
     7861 aaggctggtg aagaagcggt tccgctggaa gctggtcacc gtttttaagt gaattgccgg
     7921 atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg tcttatcagg
     7981 cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg gctgttccag
     8041 cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc ccagtaagcc
     8101 taaatgtagc gaactggttt caatgctctg cacgttttgc tgatgcaggc gatcgacgtg
     8161 ggcgtgggaa tagcggcgat taagaatgcg aaaacgatgt ttgctgcgac gcaaacgacg
     8221 agcgctggtg acatcgccag agaagaacac cgacattgcc agctttaaat tactgagcaa
     8281 ttgctcatag agcgcatcca gttccttcaa cccatcaagc gaaaatgccc gccgtgctgc
     8341 cagcgattta tcggcaattt cgctgcccat gcgctcgacg atatcggagg cctgttcaag
     8401 gttgagcgac atttcgatga tctccgccca gcggcgcgac tcttcctccg ccaactcttc
     8461 ttttggcatc cgcgccagat acagcttaat ggcggtgtag agaacgttga tatcatctgc
     8521 cagcttacgc agctctttct cctgccgtgg ctcgccgtgc atcactttat tcagcccttc
     8581 catcatctgt tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt tcgccagagc
     8641 aagcgtgggc gtgtccagcg cgctgacatc cagatgttta ggccgtagct gggtatccag
     8701 ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa caaatggcag
     8761 catgaccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca gttccgcttt
     8821 tggcaatgac agcttcccca ttgtctctgc cagcaaatgg acaaacggca ggataatcag
     8881 gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg cggcattggc
     8941 ggcactgttg ttgagcatcg ccagcaggcc ggaaccgagg ttagctccaa tcaccagaca
     9001 gagcgccacg gggaaggaga taatgcctgc agcggtcaga gtcgcggtca gcagtacagc
     9061 agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat ccagcagaat
     9121 atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga tcggcgttac
     9181 ggcctgcaca atcaactcca gcgccagcaa aatcagccca agaccaatac cgacgcggcc
     9241 cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag
     9301 taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg ccgtcccgac
     9361 atctgccccc agcacaatga ccagagccgg tgcgagggct accagatcct gagcgacgaa
     9421 cgaggtcacc agcatggtgg tggcattact gctctgtacc agtgcggtaa cgccgatccc
     9481 cgcgcaaaag gcgagcggct ttttttcgac gctccggcta aggacagtac gcaaacgcgc
     9541 gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca gcagggcgac
     9601 ggcagaaagc aggtgaagca gcgttaacac gagttcagat tctccttatc gttatatgtt
     9661 cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct ttactgtgcc
     9721 ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat aagtgtttca
     9781 tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga aaagtgacag
     9841 atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct caacatcgga
     9901 gacgctcata cctttacggc gggcatggtc ttcaacctga tcgcgctgaa tttgcgccac
     9961 cgcgtagtac ttgctgtccg gatgactgaa ataccaaccc gaaaccgatg caccaggcca
    10021 catcgcgaaa gattctgtga gtttcatgcc agtatgtttt tccacttcca gcagctccca
    10081 gatggtggct ttttccgtat gttccgggca ggccggatag cccggtgccg gacggatgcc
    10141 ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg catagcccca
    10201 gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta aacggtcggc
    10261 aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg catcagccag
    10321 tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg ctttaccaga
    10381 aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aatcccgttt tttcggtctg
    10441 ttgacgcaga tggtggctga cgttgatcac atgggtgcgc gtttcgtcac ggtagatttc
    10501 aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg gattcagcgt
    10561 tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc gctgcgcctc
    10621 aacgcccacc acttcatctt ccagaatgcg cggatacttc ccggccagcg accaggtcat
    10681 aaagaacggt gtccagtcga tgtaattacg cagcgtttcg atgctggctt ccacttcctg
    10741 cacgccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaatg cgaaatcgtt
    10801 atcgcgcgcc gcttccagcg tgaccggtgg tgtgcgtggt ttcttgcgcc cgtgctgaat
    10861 acgcacggtt tcgtactcct tgcaggtacg agcgacaaaa tcatcacgct gagtatcgga
    10921 aagcagcgcc gctaccacac caacggtgcg cgaggcattc tgcacataca ccgtagggcc
    10981 gctgtagttc tgctcgattt tcaccgccgt gtgcgctttt gaggtggtcg cgccgccaat
    11041 cagtaacgga atggcgaagc cctgacgctc catctctttc gccacgttaa ccatctcgtc
    11101 cagcgacggc gtgataagcc ccgaaaggcc aatcaaatca gcattcactt ctttggcgat
    11161 acggagaatt ttttccgcag gcaccataac gccgagatcg acaatttcgt agttgttaca
    11221 ttgcagcacc acaccaacga tatttttacc gatgtcgtgg acatcgcctt tcacggtggc
    11281 gatcaccatc ttgccgttgg ttttaccctg ctctttgctg gcttcaataa acggttcgag
    11341 atacgccact gcctgtttca tgacgcgcgc cgatttgacc acctgtggca ggaacatttt
    11401 gccttcgcca aacagatcgc cgaccacgtt catgccgtcc atcaacgggc cttcaatcac
    11461 ttcaatcggg cgcgtagcct gctggcgggc ttcttcagta tcctgctcga taaactcggt
    11521 tatgcctttg accagcgagt attccagacg tttattcacg tcccacgagc gccactccgc
    11581 ttgctgggcg ttagcagtgt cgtccgcttt actgccgcga tatttctcgg caagctccag
    11641 taaacgctca gtgccatcgt cacgacgatt aagaatcaca tcttccaccg cgtcgcgcag
    11701 ttcagcgggt aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc ccatatccat
    11761 gccattgcga atagcgtagt agaggaacac tgcgtgaatg gcttcgcgca ccggatcgtt
    11821 gccacggaat gagaaagaaa cgttagatac gccgccggaa atcagcgcgt gcggcagttc
    11881 gcgtttgatg tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt gctcttcaat
    11941 accggtggcg accgcgaaga tgttcgggtc gaagatgata tcttctggcg ggaagccaac
    12001 ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg cgcgagtatc
    12061 ggcctgtccc tgttcgtcaa aggccatcac caccactgcc gcaccgtagc gacgcaacaa
    12121 tttcgcgtga tggataaagg cgtcgacgcc ctctttcatc gagatagagt taacaatgcc
    12181 tttgccctgg atacatttca gacctttttc aatgacatcc cattttgagg agtcgatcat
    12241 aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac gcaccatcgc
    12301 cgcttcggca tcgagcatcc cttcatccat gttgatatcg ataatctgcg cgccgttttc
    12361 cacctgctga cgcgccacat ccagcgcctc gctgtatttc tcttctttga tcaggcgctt
    12421 gaacttagca gagccggtga cgttggtgcg ttcacccacg ttcacaaaca ggctatcgtc
    12481 gccaatgttc agcggctcca ggccagacaa acggcaggct acgggaattt ccggcagttt
    12541 gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgcg gcgtggtccc
    12601 gcagcaacca ccgacgatat tgagaaagcc cgcttgcgcc cattcacgta tctgttttgc
    12661 catcgtgtcg gcgtcgagat cgtattcacc aaaggcgtta ggcagcccgg cgttcgggtg
    12721 ggcggtgacg tagcattccg caatccgtga tagttcctgc acgtactggc gcagttcatc
    12781 gggccccagc gcacagttca ggccaaaggt cagcgcttcg gcgtggcgca atgagttgta
    12841 aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga tggtgccgga
    12901 gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg caaagaccgc
    12961 cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg caccaccttc
    13021 caccagcgct ttggtggatt cgcgataggc cgccgccagc tggtcaaaag tgatgttgcg
    13081 gaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggttg ggccaagaac
    13141 accggcaacg tagcgcggtt tctccggcgt gcgcgcggtc cactcgtcag cacaggcgcg
    13201 cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct ggtaatccgc
    13261 catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc ccgcttcaaa
    13321 gtaggcgttg tggatagcgg cgatcacttc cggtttactg agtaccagca ggtcgttgtt
    13381 gcctttgagg tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg cttcgttcag
    13441 tcgatagctc tggatcatgg tgcccatacc gccgtccaac accagaatac gttcatttaa
    13501 ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc ttaacccaga
    13561 tttaataaaa attcaacaaa ccatactgga ataaacgcat ctgtggtaaa agcgaccacc
    13621 acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac tattgcatta
    13681 gctaacaata aaaatgaaaa taatttccac gatacagaaa aaggaggctg tcatggtcgc
    13741 acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag cgactggaca
    13801 ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat ccaatggcag
    13861 cgtggcactc acagagctgg cgcaacaagc cgggttaccc aattccacga cccaccgcct
    13921 gctaaccacg atgcaacagc agggtttcgt gcgtcaggtc ggcgaactgg gacactgggc
    13981 aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta atttgttagc
    14041 aattgttcac cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg tcaatatggc
    14101 ggtgcttgat caaagcgatc acgaagcgat tattatcgac caggtacagt gtacccacct
    14161 gatgcgaatg tccgcgccta tcggcggtaa attgccgatg cacgcttccg gtgcgggtaa
    14221 agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc gcaaagggtt
    14281 acatgcctat acccacgcaa ctctggtgtc tcctgtgcat ttaaaagaag atctcgccca
    14341 aacgcgcaaa cggggttatt catttgacga tgaggaacat gcgctggggc tacgttgcct
    14401 tgcagcgtgt attttcgatg agcaccgcga accgtttgcc gcaatttcta tttccggacc
    14461 gatttcacgt attaccgatg atcgcgtgac cgagtttggc gcgatggtga ttaaagcggc
    14521 gaaggaagtg acgctggcgt acggtggaat gcgctgactt tttctggcgg gcagaggcaa
    14581 tattctgccc atcatacctg agtggcaata gaataagggt gtctgttaat cgcattgacg
    14641 ccaaaataac ttaatgtcat acacttcaca cacttttatt aactcagcat tatttttaaa
    14701 catcaaacca cttaattatt aacaaaatcg caagatctca ttccaccgaa acttttacgg
    14761 ctgcattatg agattgagcg atttatgata tttttttagt tgtaatcata ttaaattttt
    14821 tcactgttct gatagttaaa attcaagaca tcaataaaca atgagatatt taaatgatta
    14881 ctcgtattcc tcgtagttct ttctctgcaa atattaataa tacagcccag acaaatgaac
    14941 accaaaccct gagtgaattg ttttataaag aactcgagga taaattttct ggcaaggagc
    15001 tggcgacgcc tctattaaaa agcttctcag agaactgtag acaaaatgtt cgtcatatct
    15061 ttagcaacaa ggattttgtc attaaatttt ccacgtccgt cttacaagct gataagaaag
    15121 aaattacgat gattaataaa aacgaaaaca cgacactcac tcaaaccatt gctacaatat
    15181 ttgaagaata cctaatggaa attttacctc aacgctcaga cactcttgat aaacaagaat
    15241 taaaccaaaa atcagataga aaagaaaaag aattcccaag aattaaactt aatggtcaat
    15301 gttatttccc ggggcgcccc aaaaccgtat agtataccga cacattgctg cacaatatat
    15361 taatgatatt tatcagaatg ttgattacaa accccatcaa gatgattact cttcagctga
    15421 aaaatttctc actcacttca acaaaaaatg caaaaaccag actttggcgt tgatttccag
    15481 ccgtcctgag gggcgttgcg ttgctgcctg cggtgatttc gggctagtta tgaaagcata
    15541 ttttgacaag atggaatcaa atggcatcag tgttatggca gccatattac tggtggataa
    15601 ccatgctttg acggtccggc taagaataaa gaacacaact gaaggatgta cccattacgt
    15661 ggtttcggtt tatgatccta atgtaactaa cgataaaata agaattatga gcgaaagcaa
    15721 agaggatatt aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa
    15781 atggtcaaat gatcatgtta ttaaccaatc tgttgcaata attccagcac ttccgaaaga
    15841 acagctattg atgttaaaag gaactgtgga tgaaataacc cctccattat caccagcaac
    15901 gatgaatttg ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcagct
    15961 ccagaaaatg ccagaactac atagaacaga aatgttgact gcctataata gtattaacct
    16021 ccccggttta tatttggcta taaattatgg caatgcggat atcgttgaga ctattttcaa
    16081 ttcattgtca gaaacaggat atgaagggtt actctcgaaa aaaaatctca tgcatattct
    16141 ggaggcaaaa gataaaaatg gtttttctgg attattttta gcgatatcac gtaaggataa
    16201 aaatgttgta acctcgattc tgaacgtctt acctaaactg gccgcaacac atcatttaga
    16261 taatgaacag gtgtataaat tcctgagtgc caaaaataga acgtccagcc atgttttata
    16321 ccatgttatg gcgaatggtg atgccgacat gctgaaaatt tttttggtcg cgttaccttt
    16381 gttaattcgc acatgtcatt tgactaaaga acaggtactc gatctcctga aggcaaagga
    16441 tttttatggt tgcccaagac tatacctggc gatgcaaaat ggacatagcg atatcgtgaa
    16501 agttattctc gaagcattgc cctgtctggc ccaggaaatt aacatttcag cttccgatat
    16561 tgtagatctt ctgaccgcta aaagtcttgc gcgcgacacg ggtttgttta tggccatgca
    16621 gcgcggacat atgaacgtta ttaatactat ttttaacgca ttacccactc tttttaatac
    16681 gtttaaattc gataaaaaaa tatgaagccc ctcctcctgg caaataattc taatgaatat
    16741 cccggtttgt tttcagcgat acagcataaa caacaaaacg ttgtagagac ggtttatctt
    16801 gctttatctg accatgcacg cctgtttgga tttaccgctg aagatattat ggatttttgg
    16861 caacacaaag cgccacaaaa atactctgcc tttgagttgg cttttgaatt tggtcaccgg
    16921 gttattgctg aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg
    16981 gataaccctc gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct
    17041 ccccataccg tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc
    17101 cttcacgtat gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca
    17161 tctcttcaaa cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg
    17221 ggaaaacatc gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat
    17281 agcgcggcgg cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat
    17341 cataaaaaac cacacgcccg tgacgggtga caccgaagtt tttaaacagc atgtcgccag
    17401 ggaaaatgtt agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc
    17461 gcaactgctg accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga
    17521 ttatagagat ggcgaattac aatctgctca ccaagatcgg tgattttttc cgctgcttcc
    17581 tggagcagta attccattaa taccggggaa atatgccgct tctccagcac aaagttttca
    17641 aactcctggg tgtccgccat tcggcccaca cggtcgtgct ctttcaccag ttgatagcag
    17701 gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgactttg
    17761 aatacccgat caaagcccgg cagcgtaaac accaacatca ccattccacg aatacccggc
    17821 gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg
    17881 gttttggcgt gcttctggca gccgatagcc atatacaatt cagcagtggt tttacctggc
    17941 agaatttccc gtagccactc gaccagcgct gcgggcagcg gtgcataaac cataaaataa
    18001 gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata
    18061 aataactcgc cgtcgtccgt ctggtggatc ggcagtaaga agggcaacgt gccggaaggc
    18121 gtgatcagtt tgcccaccag ccaggcggct ttattgcggt aaaacagttc gttcgccacc
    18181 tgtaaatgac tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg
    18241 taatgaatgt cacggctttt attctgccag cgcaggcgca gcggcaggtc gctgataacg
    18301 cgcatcagta gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga
    18361 atggtacgaa agcggcgctc tggctgagag ctaaagataa aaagccgctc gggagtaagc
    18421 gagcggtggt caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag
    18481 cgcgggtaat ccggcaacag ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg
    18541 tccgtacttt ggccgttagt aatgcagcgc agttgctcca cgaccagacc aacgtgatga
    18601 tcgtaaagat ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcctgttcg
    18661 aaacgctgct gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct
    18721 tgcaaaatgg tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag
    18781 gagcagagaa ttgccggatg cggcgtaaac gccttatcca gcctacgttc ggcaacggtt
    18841 gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg
    18901 gcgtaaatgc cttatccggc ctacagtcag caacgtttgt aggttgctta gaactgcgat
    18961 tcttcagtgg agccagtcag cgcggtgact gaagacgtgc cgccctgaat aatagtcgtc
    19021 actttatcga agtaacctgt acccacttcc tgctggtgag atacgaaggt atagccatct
    19081 ttcgcggcgg caaattccgg ctgctgcact ttctcaacat agtgcttcat accttcgccc
    19141 tgcgcataag cgtttgccag gtcgaacatg ttaaaccaca tgctgtggat acctgctagg
    19201 gtgatgaact ggaacttgta gcccatatcc gacagctgct gctggaagct ggcaatagtt
    19261 ttgtcgtcga ggtttttctg ccagttgaac gacggcgagc agttataagc cagcagtttg
    19321 cccggatatt tcgcgtggat agcttgtgca aagcgacgcg ccagttccag atccggcgtg
    19381 gaggtttcac accagaccag gtcagcatat ggcgcatacg ccaggccacg gctgatcgct
    19441 tgctcaatgc ccgcatgagt acggaagaag ccttcactgg tacgctcgcc ggtaataaat
    19501 tcgctgtcat acgggtcgca atctgaggtg atcagatccg ccgcatcagc atcggtacgt
    19561 gcaaccagca gggttggaac gcccgtcacg tcagctgcca gacgcgccgc gaccagtttc
    19621 tgaatagctt cctgagttgg cactaaaact ttgccgccca tgtgaccgca tttcttcact
    19681 gacgccagct gatcttcgaa gtgaactgcc gcagcaccgg cttcaatcat cgctttcatc
    19741 agttcaaagg cattcaggac accgccaaaa ccggcttccg catcggcaac gatcggcagg
    19801 aagtaatcga catagcgcgg atcgcccggc tcaatgccca cggaccattg gatctgatcg
    19861 gcacgacgga aggtgttgtt gatccgctcc accacagctg gcaccgagtt tgccggatag
    19921 agcgactgat ccggatacat gctggccgcc aggttagcgt ccgccgctac ctgccatccc
    19981 gacagataga ctgcttcaat acccgctttc gcctgttgca gcgcctgacc gccagtcagt
    20041 gcaccgaggt tgttgatgta gccttttttc gactcaccgt gcagcagacg ccacattttc
    20101 gctgcgccca gttgcgccag cgtgcattca ggattgactg aaccgcgtaa tttcaccaca
    20161 tcttccgcac tgtatgggcg agtaatgcct tcccaacgcg gttgagtcca ctctttctgt
    20221 aattcttcaa tttgttgtgt acgggttttc atgtgcagat gctccatagt tatgtggtgg
    20281 tttacgctaa caggcggtag cctggcaggg tcaggaaatc aattaactca tcggaagtgg
    20341 tgatctgttc catcaagcgt gcggcatcgt caaaacggcc ctgggagaaa cgttcttcgc
    20401 ccagttcgct ggcaatgact ttcatctctt cgcccagcat ctggcggaac aaggctttgg
    20461 tcaccggttt gccattgctc aacgtttttt gatgatggat ccactgccag atcgaggtac
    20521 gggaaatttc agccgtcgcc gcatcttcca tcaggccata aatcggcacg cagccgttgc
    20581 cggagatcca cgcttcgatg tactgcacag ccacgcgaat gttagcgcgc ataccttctt
    20641 cggtgcgttc accatcacaa ggtgccagca gctgatcggc agtaatcggc gcgtcttgtt
    20701 cgcgcaatac ttcaagctga tttttacggg agccgagaat gtcgttgaat accgccattg
    20761 ccgtgtccgc aaggcctggg tgagcgatcc atgtgccatc gtgaccgtta ttggcttcca
    20821 gcgctttatc cgcttttact ttgttgagca cctggttatt acgctcttca tctttgctcg
    20881 gaataaacgc ggccatgccg cccatcgcaa aagcaccgcg tttatggcag gttttaatca
    20941 gcaggcgtga gtaagcattc aggaatggtt tatccatcgt cacggcctgt ctgtctggca
    21001 ggacacgatc gggatagttt ttcaacgttt tgatatagct gaagatgtaa tcccagcgac
    21061 cgcagttcag accaacaata tggtcgcgca gcgcgtgaag gatttcgtcc atctggaaca
    21121 cggcgggcag cgtttcaatc agcaacgtcg ccttgatggt gccgcgcggc agattaaagc
    21181 gatcttctgc atagctgaag acttcgctcc accaggccgc ttcctgctag gactgggttt
    21241 tcggcagata gaaataggga ccgctgccct ttgccaacag tgcctgatag ttgtggaaga
    21301 aatagagcgc aaaatcaaac aggctaccgg ggattgcctc accacgccag gtgacatgtt
    21361 tttccggcaa gtgcagaccg cgtacccgac aaatcaaaac cgctggattg ggcttgagct
    21421 gataaatttt gcctgcttca ttggtatagc tgatggtacc gttaaccgca tcgcgcaggt
    21481 taatttgccc gtcgatcact ttgttccagt ctggtgccag tgaatcttcg aaatcggcca
    21541 taaagacttt cacattggcg ttcagtgcgt tgatcaccat cttgcgctct accgggccag
    21601 ttatctctac gcggcggtct tctaagtccg caggaatccc gcgaattttc caatcagcat
    21661 cgcgaatgga agccgtttcc gaaataaaat caggcaacgt tccgttatca atatcttgct
    21721 gctgctgaat gcgcgctgcc agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct
    21781 cagtcagaaa ttctaccgct tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc
    21841 ttgtgaaagc cagttcatcg gttgttgttg cctgttcagt catcgtgcag ctcctcgtca
    21901 tggatccgaa aacttcccct ctgtgaacga aggaacgttg tgcgcttttc gttcagcaca
    21961 actaagacta ctcatttaaa atgcaaaatc aaaaacaatt tccattttaa ttaaaaatac
    22021 ttgataactt attgataaca atatgaataa aatttattca cttgctgagg tgcgtttcgg
    22081 aaataaaaaa ggcacccgaa ggtgcctgag gtaaggtgct gaatcgctta acgatcgact
    22141 atcacagaag attaatccag cgttggattc atgtgccgta gatcgtatgg cgtgatctga
    22201 tagacgtaat agttgagcca gttggtaaac agcaaattac cgtgactacg ccagctcgct
    22261 cgcggtttgt tttgcggatc gttgtgcggg aaatagttat acggtacatc cgggtctagt
    22321 ccggcttcca catcgcgtaa atattcctgc gccagcgttt gcgcatcata ttcgggatgg
    22381 cccgtcacaa aggcaatgcg cttatctttg ctggcaaaca gatatgcatc cccttcttcc
    22441 gtctctgcca gaatttccag atcggtgtaa tcacgaatca acgctgccgg aaagtcagca
    22501 tagcgcgaat gcggtgccag gaatgaatca tcaaagccac gcgtcagaag cgcatgagga
    22561 tggagaatat gatgctcgta aacgccagag agtttgtcgg tgcgagtttg cttaggaatg
    22621 ccgtagagga tattgagcgc ggcctgtacc gcccagcaga caaacagcgt cgaggtgacg
    22681 tgatctttcg accactccag cacctgtttg atctgcggcc agtaagcgac atcattaaac
    22741 tccaccaggc ccagcggcgc accggttaca atcagaccgt caaagttctg ttcctgaata
    22801 tcttcaaagt tacagtagaa gttgttcaga tgctctgcgg gcgtgttgcg cgattcacgg
    22861 gaatcgatgc gcaacagctg aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga
    22921 aactgatttt cagtttcaat cttcttcggc atcaggttaa ggatcagaac cttaagtgga
    22981 cgaatttcct gaccagacgc acgagaagtt gtcatcacaa agacgttttc ttcacgcaag
    23041 aaattgacgg cgggtagctc gtccggcaca cgaatcggca taacctgatt acctcactac
    23101 atacgcttat acgtttagac atccagatag ctgaagataa ccagaaaatt tgccaatgtc
    23161 gagcctgcat gttgaaggtg agaaagtttc aaggaaaagg ctacgttaga atataagaat
    23221 gacgaaaagg agagaaaatg gttattagta ttcgccgctc acggcatgag gaaggggagg
    23281 aactcgttgc gatttggtgt cgatctgtcg atgccaccca cgatttttta tcagcagagt
    23341 atcgggccga gctggaagag ctggtgcgtt cctttctgcc ggaagcaccg ttgtgggtcg
    23401 cggttaatga acgggagcag ccggttgggt ttatgttgtt aagtgggcag catatggatg
    23461 cgctgtttat cgatcctgat gtgtgcggct gcggcgtggg tcggatgctg gtggagcatg
    23521 cgctctcgat ggcaccggaa ctgacaacca acgttaatga gcaaaatgag caggcggttg
    23581 ggttctataa gaaggtgggt tttaaggtta cgggacgctc tgaggtggac gatttgggga
    23641 aaccgtatcc gttgctgaat ctggcgtatg tgggggaata agccgcctct gccgtgcctg
    23701 cccaagctgg agaagacg
//
LOCUS       DAJTNP010000059        22446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTNP010000059 DAJTNP010000000
VERSION     DAJTNP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            72..2672
                     /locus_tag="OO160_002516"
     CDS             72..2672
                     /locus_tag="OO160_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5524591.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2663..3646
                     /locus_tag="OO160_002517"
     CDS             2663..3646
                     /locus_tag="OO160_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5524592.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3744..4284
                     /locus_tag="OO160_002518"
                     /pseudo
     CDS             3744..4284
                     /locus_tag="OO160_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4292..4807
                     /locus_tag="OO160_002519"
     CDS             4292..4807
                     /locus_tag="OO160_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5524593.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4773..5510
                     /locus_tag="OO160_002520"
     CDS             4773..5510
                     /locus_tag="OO160_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5524594.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5621..6631
                     /gene="pyrD"
                     /locus_tag="OO160_002521"
     CDS             5621..6631
                     /gene="pyrD"
                     /locus_tag="OO160_002521"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5524595.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6805..7347
                     /gene="zapC"
                     /locus_tag="OO160_002522"
     CDS             6805..7347
                     /gene="zapC"
                     /locus_tag="OO160_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5524596.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7344..8453)
                     /gene="ycbX"
                     /locus_tag="OO160_002523"
     CDS             complement(7344..8453)
                     /gene="ycbX"
                     /locus_tag="OO160_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5524597.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8697..10805
                     /gene="rlmKL"
                     /locus_tag="OO160_002524"
     CDS             8697..10805
                     /gene="rlmKL"
                     /locus_tag="OO160_002524"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5524598.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10817..12724
                     /locus_tag="OO160_002525"
     CDS             10817..12724
                     /locus_tag="OO160_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5524599.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12854..14107
                     /gene="pqiA"
                     /locus_tag="OO160_002526"
     CDS             12854..14107
                     /gene="pqiA"
                     /locus_tag="OO160_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5524600.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14112..15752
                     /gene="pqiB"
                     /locus_tag="OO160_002527"
     CDS             14112..15752
                     /gene="pqiB"
                     /locus_tag="OO160_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5524601.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15749..16312
                     /gene="pqiC"
                     /locus_tag="OO160_002528"
     CDS             15749..16312
                     /gene="pqiC"
                     /locus_tag="OO160_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5524602.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16568..16735
                     /gene="rmf"
                     /locus_tag="OO160_002529"
     CDS             16568..16735
                     /gene="rmf"
                     /locus_tag="OO160_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5524603.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16805..17323)
                     /gene="fabA"
                     /locus_tag="OO160_002530"
     CDS             complement(16805..17323)
                     /gene="fabA"
                     /locus_tag="OO160_002530"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5524604.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17392..19152)
                     /locus_tag="OO160_002531"
     CDS             complement(17392..19152)
                     /locus_tag="OO160_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5524605.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19337..19789
                     /gene="matP"
                     /locus_tag="OO160_002532"
     CDS             19337..19789
                     /gene="matP"
                     /locus_tag="OO160_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5524606.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19865..20911)
                     /gene="ompA"
                     /locus_tag="OO160_002533"
     CDS             complement(19865..20911)
                     /gene="ompA"
                     /locus_tag="OO160_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5524607.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21267..21776)
                     /gene="sulA"
                     /locus_tag="OO160_002534"
     CDS             complement(21267..21776)
                     /gene="sulA"
                     /locus_tag="OO160_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5524608.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21995..22405
                     /gene="sxy"
                     /locus_tag="OO160_002535"
                     /pseudo
     CDS             21995..22405
                     /gene="sxy"
                     /locus_tag="OO160_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5730 a         5269 c         5880 g         5567 t
ORIGIN      
        1 tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc gtcttcagtt
       61 aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct ggtggagtgc
      121 catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca caagctgaaa
      181 cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat ttgtctcgtt
      241 tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt ctgaatcaga
      301 caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag gggattgccg
      361 cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg tttccggctt
      421 ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg gatgccagcg
      481 taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc gccatcaaaa
      541 gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac gcgctattac
      601 tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt gattctggcg
      661 acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga ttacgcaaca
      721 attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc agcagctatt
      781 tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat tatactgcag
      841 gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat gacaacatgc
      901 tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa agcaatgccc
      961 agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg cctggtgctt
     1021 ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt gaaatcaaag
     1081 aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca ttactccagc
     1141 gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat agtaatgaac
     1201 agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg ggaacgacat
     1261 ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt ctgggattta
     1321 accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt acgctggcag
     1381 accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg ctcaaccaat
     1441 tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc tacacccttt
     1501 ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat gatgaagata
     1561 cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa ctgcaggtca
     1621 atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc cagcaaactt
     1681 actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg caaattaaag
     1741 atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct gatgctgatc
     1801 aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga agtaatgata
     1861 gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc gataacgaag
     1921 ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt aacctgagct
     1981 acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc gccagcatgg
     2041 actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat aacggcagtc
     2101 aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag ggcattaccc
     2161 tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca gagaatactc
     2221 gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt gtcccctatg
     2281 ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa cgtaacgtgg
     2341 atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt ctggcggagt
     2401 tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt ataccgctgc
     2461 gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt attgatgatg
     2521 atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta cgctggggcg
     2581 aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa attaactctg
     2641 cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg tgttattact
     2701 acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg cttatatcgg
     2761 gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg ttaaaactct
     2821 ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt ggaacgaatc
     2881 gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg gccgatgggc
     2941 attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa ttaatgacta
     3001 tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg ttcctttcga
     3061 aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg gtcaacgttt
     3121 aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat tgcagccgtt
     3181 cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat gctacaacat
     3241 acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt acctgtctgg
     3301 tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg aggttgatct
     3361 gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg gggccagaac
     3421 ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag ttaacgtcaa
     3481 tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga cgtcacgtcc
     3541 tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg ctggtgggac
     3601 actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt ctatccagtc
     3661 agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata
     3721 aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc gttattttgt
     3781 tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc agtatgcata
     3841 tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata
     3901 ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta agcactgctg
     3961 ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg gacgtccttt
     4021 ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac acaggcggaa
     4081 gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca gcaagaaatc
     4141 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
     4201 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
     4261 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
     4321 cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg tcaacattag
     4381 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
     4441 gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg agaaatcttt
     4501 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca ccttttcagg
     4561 aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg atgcagccag
     4621 tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
     4681 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
     4741 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
     4801 tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg ctgctggtgg
     4861 cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
     4921 gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt attaacagca
     4981 aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca gcactattac
     5041 tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg cgcttgctga
     5101 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
     5161 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
     5221 aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat caacaattac
     5281 gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac tacattaacc
     5341 tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg ccgcctaaaa
     5401 gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc tggcggacga
     5461 taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga aacaggttct
     5521 gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa ctcgcacata
     5581 atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc ccttcgttcg
     5641 taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc agcaattacg
     5701 ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg cgaaacctgt
     5761 taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc ttgataaaga
     5821 cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga tcggtaccgt
     5881 cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg tagatgccga
     5941 aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg tagagaacgt
     6001 aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata aagatacgcc
     6061 agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg cctatgcggg
     6121 atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc tgcaatatgg
     6181 tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt tgcaagcgat
     6241 gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg aagacgaatt
     6301 gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg caaccaatac
     6361 cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg gtggcttaag
     6421 tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac tagaattaaa
     6481 cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg cgcgtgaaaa
     6541 gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta aaggtccgcc
     6601 gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc tttataagat
     6661 aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc ttatttagac
     6721 attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt gttgctgcgg
     6781 ttttataggg taggggagag gcagatgcga attaaaccag acgataactg gcgttggtat
     6841 tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct atttcgctca
     6901 cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt ttgtgttgat
     6961 gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt atctaaagac
     7021 caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa accgcaaatg
     7081 ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc gggcgatgcg
     7141 gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt tgaatctggc
     7201 gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg tcgcgcgatg
     7261 cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt gaatgttgac
     7321 agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt cggtacacag
     7381 ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg cgtgacttcg
     7441 ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga ataagggata
     7501 cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc acgaaatgcc
     7561 tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt ggcggtatca
     7621 tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc cacctcatcg
     7681 ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc gacatcgccg
     7741 ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc cggatgtttt
     7801 tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt aaccacatca
     7861 aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc tgacgcgccg
     7921 gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca acgttgttgg
     7981 agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc aaatgacaga
     8041 ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc
     8101 acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc aattcgcgcg
     8161 gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga
     8221 acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac aggtgaagga
     8281 gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc aggttccgtg
     8341 atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg tgtaagacca
     8401 atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc cacggtgcgg
     8461 tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag ctataatgcg
     8521 caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt ggccctgggt
     8581 tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc tccatgtcca
     8641 gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt gacgatatga
     8701 attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact gaactggaaa
     8761 atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag ggcgacacac
     8821 ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg ttgccgctgg
     8881 gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg atcaactgga
     8941 cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg aatgacacca
     9001 tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat gctttcacac
     9061 ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct
     9121 ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt ttacatctgc
     9181 gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca gccgctattg
     9241 tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt ggttccggta
     9301 cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg caccgtgggc
     9361 gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa gtgaaagcgg
     9421 aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt tacggttcgg
     9481 acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg gggattggtg
     9541 aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg ccgaaagggc
     9601 cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc gaaccggcgc
     9661 tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt ggctggaatc
     9721 tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt gcagacaaac
     9781 agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac gttgccgaaa
     9841 gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc ctgcgtaaga
     9901 acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac cgcctgtatg
     9961 acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc
    10021 aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt ctgttcgata
    10081 ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc
    10141 gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc gagtttcttg
    10201 aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc gatactggtc
    10261 tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa ggcaaagatt
    10321 tcctcaacct gttttcttat accggcagcg ctaccgtgca tgcgggatta ggcggtgcac
    10381 gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa cgcaacctgc
    10441 gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc
    10501 tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc ttctctaact
    10561 caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg atgaaagatt
    10621 tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa cgtggcttcc
    10681 gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt acgcaaaaaa
    10741 cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg attaccgcag
    10801 cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt cgttcagcga
    10861 cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg tttgtctggt
    10921 gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct
    10981 ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc aacaggatcc
    11041 gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg aagaacaagc
    11101 ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc cgagcgagaa
    11161 aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc tgtggcagct
    11221 ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg ttgcgctgtc
    11281 gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg tgagcaatcc
    11341 gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca tcgactggct
    11401 ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg accgttcgtt
    11461 tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg tgacctatcc
    11521 agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg aagaactaca
    11581 aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc aggggatcaa
    11641 agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc gtcgcgaacg
    11701 tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg ccagccgctc
    11761 cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta agcaactggt
    11821 gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg gtccgaatgg
    11881 gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg acagcgggcg
    11941 tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg cggaactgga
    12001 tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga tggttaatgg
    12061 caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga aacgggcgat
    12121 gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt
    12181 cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg atgtcgaaac
    12241 gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc tggttagcca
    12301 cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag gcggcggtaa
    12361 aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac aatatgtggc
    12421 actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag cagaaactgt
    12481 aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc agctaccgca
    12541 attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg cggatgcttc
    12601 cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg ctgctgcaga
    12661 gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg
    12721 ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat tgcctgatga
    12781 actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa tagcgcatca
    12841 ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt gctcgcagtg
    12901 tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat gtccccggtg
    12961 tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg cctatgcgtt
    13021 ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc
    13081 aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt ctgaggacta
    13141 cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat
    13201 aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag agcaactggc
    13261 acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg cgggtgtgct
    13321 ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca gctttctccc
    13381 ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt
    13441 atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag gcgtcacggg
    13501 gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg ctgatgaacc
    13561 cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc tgcagtggac
    13621 actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt tgcccatcat
    13681 ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt
    13741 atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc
    13801 aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc atggcaagcg
    13861 cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc gctggtcgat
    13921 gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag gtttaatgag
    13981 tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga caatgttttc
    14041 tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga
    14101 gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg aagaactggt
    14161 ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt cttttttatc
    14221 attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag
    14281 gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac
    14341 tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt atggaaaaat
    14401 tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc gaagggatta
    14461 gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc gcgaaaggca
    14521 gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct gatgcgaaag
    14581 gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga gatccggtgc
    14641 tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg caaaaacgca
    14701 atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc agcaatgttc
    14761 gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg cgtgtggaga
    14821 tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg gaaggtctgg
    14881 atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat cagaagagca
    14941 ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa gattcggtac
    15001 gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt accgtaagca
    15061 aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac cgtattccgg
    15121 tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat gcggatgttg
    15181 ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc
    15241 tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct gcaataaccg
    15301 gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc ttggcgcaaa
    15361 tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg aatccgatga
    15421 ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga
    15481 cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg ccgacggata
    15541 tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct ggctccgcag
    15601 cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga gaactgcaac
    15661 cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg aaggacaaaa
    15721 aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact
    15781 gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt tacctgtggt
    15841 gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg tagagcaggt
    15901 cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg atgtgaagta
    15961 tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc gcaacaccct
    16021 ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc ctctgggaag
    16081 cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt
    16141 cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac gtccgttccg
    16201 tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc tggccggtgt
    16261 ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt aattataaag
    16321 atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt ttctaacgtg
    16381 cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg tagctcacaa
    16441 atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg tattgcttaa
    16501 ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag tatgagggaa
    16561 acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac gtggttatca
    16621 ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga gtcaaaggtc
    16681 acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg cctgattctg
    16741 tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact ctgtctgatg
    16801 ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg gcggtataga
    16861 tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga cgacgattaa
    16921 caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt acctgaccag
    16981 tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc agccagccga
    17041 ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc ataaccggat
    17101 cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt tctgcttcaa
    17161 catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg tctatcatca
    17221 gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg tgaccagagg
    17281 caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct gtaagcctta
    17341 ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg atcagttcgg
    17401 aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga
    17461 tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt cgccatccca
    17521 caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc agatggtgaa
    17581 tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc gaacgttagc
    17641 agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa tagcaaagaa
    17701 gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact gatcgactga
    17761 acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta tcagggcgca
    17821 gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct caaatgtcag
    17881 cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga acgcctgcat
    17941 gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt
    18001 gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg
    18061 tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc
    18121 ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag cgaggaaacc
    18181 ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc
    18241 atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc taagcggaag
    18301 tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc aagcatccgc
    18361 tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac accactggct
    18421 tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa tagcctgctc
    18481 tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc ctaccagaat
    18541 gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg actcatcaaa
    18601 cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc acagcagagg
    18661 ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct gatgcaccag
    18721 accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga gttgctccgc
    18781 ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat ctgcactcac
    18841 tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca ccagttggcc
    18901 tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact cagactcttc
    18961 cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact gctccagcgc
    19021 aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg
    19081 ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc atgcaagttt
    19141 cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc
    19201 ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt aaagctcaca
    19261 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    19321 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    19381 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca tagaagccag
    19441 tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg tgctggtaaa
    19501 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc agaccattcg
    19561 ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    19621 tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg gtaaaacgct
    19681 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    19741 aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    19801 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    19861 taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    19921 gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    19981 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    20041 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    20101 aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg tctttcggat
    20161 ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    20221 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    20281 ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa cggtaggaaa
    20341 cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg tcaccgatgt
    20401 tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg tactcaacac
    20461 cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg ccaccaggta
    20521 cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag atgtccagat
    20581 cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct ttgtatgcgc
    20641 cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc atttcaaaac
    20701 caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg ttttcgtggg
    20761 tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc agtttagcac
    20821 cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg
    20881 ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca tccaaaatac
    20941 gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga tctgctcaat
    21001 attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta aagtctacaa
    21061 cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa gtcttgtata
    21121 aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg aaaccgcttc
    21181 agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa
    21241 agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg
    21301 gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa ccccatagcg
    21361 ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgtc
    21421 aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccact
    21481 gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc
    21541 tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc
    21601 tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg
    21661 cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt
    21721 ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc
    21781 aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca acaaagatca
    21841 accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta
    21901 atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat
    21961 cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat
    22021 ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg
    22081 cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta
    22141 tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac
    22201 atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg
    22261 gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga
    22321 gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct
    22381 ggaagcgatt ctcggggagg tgggtagact ggccccctga atctccagac aaccagtatc
    22441 acttaa
//
LOCUS       DAJTNP010000060        22433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTNP010000060 DAJTNP010000000
VERSION     DAJTNP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="OO160_002536"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="OO160_002536"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5524609.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="OO160_002537"
     CDS             691..1317
                     /locus_tag="OO160_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5524610.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="OO160_002538"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="OO160_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="OO160_002539"
     CDS             3452..4090
                     /locus_tag="OO160_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5524611.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="OO160_002540"
     CDS             4154..4822
                     /locus_tag="OO160_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5524612.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /locus_tag="OO160_002541"
     CDS             4819..5604
                     /locus_tag="OO160_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5524613.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /locus_tag="OO160_002542"
     CDS             5608..6420
                     /locus_tag="OO160_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524614.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..7854)
                     /locus_tag="OO160_002543"
                     /pseudo
     CDS             complement(6432..7854)
                     /locus_tag="OO160_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7980..8285)
                     /locus_tag="OO160_002544"
     CDS             complement(7980..8285)
                     /locus_tag="OO160_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5524615.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8393..8469)
                     /locus_tag="OO160_002545"
     tRNA            complement(8393..8469)
                     /locus_tag="OO160_002545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8433..8435),aa:Val,seq:gac)
     gene            complement(8474..8550)
                     /locus_tag="OO160_002546"
     tRNA            complement(8474..8550)
                     /locus_tag="OO160_002546"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8514..8516),aa:Val,seq:gac)
     gene            8858..10114
                     /locus_tag="OO160_002547"
     CDS             8858..10114
                     /locus_tag="OO160_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5524616.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="OO160_002548"
     CDS             complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="OO160_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5524617.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11743..12384
                     /gene="ribE"
                     /locus_tag="OO160_002549"
     CDS             11743..12384
                     /gene="ribE"
                     /locus_tag="OO160_002549"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5524618.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="OO160_002550"
     CDS             complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="OO160_002550"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5524619.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13863..15074)
                     /locus_tag="OO160_002551"
     CDS             complement(13863..15074)
                     /locus_tag="OO160_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5524620.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15187..16119
                     /locus_tag="OO160_002552"
     CDS             15187..16119
                     /locus_tag="OO160_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5524621.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16116..17141)
                     /gene="purR"
                     /locus_tag="OO160_002553"
     CDS             complement(16116..17141)
                     /gene="purR"
                     /locus_tag="OO160_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5524622.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17440..17529
                     /gene="ynhF"
                     /locus_tag="OO160_002554"
     CDS             17440..17529
                     /gene="ynhF"
                     /locus_tag="OO160_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5524623.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17695..18864
                     /locus_tag="OO160_002555"
     CDS             17695..18864
                     /locus_tag="OO160_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524624.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="OO160_002556"
     CDS             complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="OO160_002556"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5524625.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="OO160_002557"
     CDS             complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="OO160_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5524626.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20881..21228
                     /gene="grxD"
                     /locus_tag="OO160_002558"
     CDS             20881..21228
                     /gene="grxD"
                     /locus_tag="OO160_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5524627.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21267..21767)
                     /gene="tnpA"
                     /locus_tag="OO160_002559"
     CDS             complement(21267..21767)
                     /gene="tnpA"
                     /locus_tag="OO160_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5524628.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21775..>22433
                     /locus_tag="OO160_002560"
     CDS             21775..>22433
                     /locus_tag="OO160_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5524629.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5813 a         5216 c         5808 g         5596 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taagtgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgcttgaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 cctggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcagatc cccgcttcac gtaaggccag
     6661 tagtcgacga atagactctg aagggattgc cgcataatta tcgatgaaaa ccctggcaag
     6721 gcctttactg aaccgtttat ggtcgtgttc attcagatga ggaacaattt cctgtacggc
     6781 ttcatgcagg cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg
     6841 ttcgacttcc tggagatttt tttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc
     6901 aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca
     6961 gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc
     7021 ctcttcacct ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt gtaagggctc
     7081 gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg acatcaacgt
     7141 aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt tatcatcttc
     7201 aatgaacgaa ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt
     7261 tcccataata cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct
     7321 gggaaaatac gttctggttg cttcttcttc atcaggccag acgtgacccg tggcaatcac
     7381 cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga cgccagcatt
     7441 tgtaatttgc agatcggtaa cctggcatga ttcataaaca gccactgcaa atttttgctg
     7501 tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa tattcgccca gcagaattcg
     7561 cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg ccataacgct ggagatggct
     7621 ggcttcttgc ttttgtagcc attcgagata cgtacaatta atcggcggta tttcaatact
     7681 ggcaatattt gccagcatca tttttgagtt ttcctcatca ctgtatggca tcccgacacc
     7741 ggcctcgtca gcctgctcga agatagaaat agaaagtgga gtttgttgct gtagaagcga
     7801 gaataaggtg tagatccccg taggcccggc acccacaata gcaatttttt tcattgttac
     7861 ttacctgtac gcattctttc gaatttagtg ttatcagata agtttggttg agatttttag
     7921 aatttgagaa atttcagaca gggatgaaaa gggcgctaac cgcccttgat taaattctgt
     7981 caggcgagtt ttgcctgatt gatttgacta agtggctcat tgacatcgaa aactttggcg
     8041 acaacttcct caacgccgag atttttcagt cgggcagtat gtttttccag ataggcaagg
     8101 gcgctttttt catcagtgaa aaggtagatc ccaccggctt cgtggttctt ttcactttct
     8161 gtccatacct tccacagaaa accaggttcc tgattaatcg actcagcaag tggtttaagc
     8221 tgctcagcca ttgcgtcgcc aaatgggcca ttaaaagcaa aatgaagttg taacaaagtt
     8281 gccatagcga ttctccttct gataacgcat acgaagacag tgtaaatctt ttaacgtatt
     8341 ggatacagag aagaaacaga gggaaaaagg aaaagaccag attaagaaaa tctggtgcgt
     8401 ccgagtggac tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa ccaactgagc
     8461 tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac catgtcaagg
     8521 tggtgctcta accaactgag ctatgaacgc aacgttgtag gtgacaacgg ggacgaatat
     8581 tagcggcaga gtggaaaggt ggcaagaggc aaaacgtaat tttctgcgct atttcgaccg
     8641 tttgcagagc ttttaagcaa attggctata ttttgttgat ttgcaagggc gatttttatt
     8701 caggatcgca tttacatctg atacaaccca ataattcgtg tatcagagtt taaacagagt
     8761 tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta cacttaggta
     8821 gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg cgaaaaactt
     8881 gatgagcgac gggaatgtgc aaattgttaa gaccggcgag gtccttggcg cgacgcaact
     8941 tactgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg tggtcacggg
     9001 ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt acggtaacaa
     9061 tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt ccgagggagg
     9121 cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag gtgaattcag
     9181 cgttgatcag ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc tgcgtgtact
     9241 ggaaggacat cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt tggttaatgg
     9301 gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc caagtggaga
     9361 agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggccggga aagccagtga
     9421 tacgttgctt gctggtggca ccatgaataa tctcggtggt gaagactctg acactattgt
     9481 tgagaatgga tccatttatc gtctggggac ggatggcctt cagctctaca gttccggtaa
     9541 gacgcaaaac ctgtcggtga atgtgggggg gcgggctgaa gtgcatgccg gtacgctgga
     9601 aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg cggacgaaaa
     9661 ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg cattactgga
     9721 cggcgcttca atgattattg gttatggcgc agagctgcaa caatcaacga ttactgtaca
     9781 gcagggcggt gtgttgattc tcgacggcag tacggtaaaa ggtgacagcg tcactttcat
     9841 tgttggtaac atcaatctga atggcggaaa actgtggctg atcactggtg cggcaacgca
     9901 tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc aaaccagtgc
     9961 gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa gttaacgggg atatacgcgt
    10021 tgagataaca gatgccagtc ggcaaactct gtgtaacgct ctgaaattac agccagacga
    10081 agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaacc cgtgaaggtt
    10141 gcgccttgtc tttattagcg ggacgctcgt tgcagaatga tggctgacgg catacgttgc
    10201 aggaaccgca tacgcagcat catcataatg gctgccgatg tcaggccgat aataaagcct
    10261 atccagaagc ctgctggccc cataggttca acgaccagat cggtcagtgc cagaatatag
    10321 ccgcttggca agcccagcac ccagtaagct gtaaaggtga tatagaaaat ggaacgcgta
    10381 tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga aatctgatat
    10441 accgccgcca gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt gtcgttgtac
    10501 aacagggcga tttgctcccg cagtgaaacc gtgaaaatgg ccgtcagggt tgccatacag
    10561 acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga gccctgaccc
    10621 agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag cacaaacatc
    10681 agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc gagcggagac
    10741 actaacagag ccacgacggc aaacagtgtc acttcaaaga acagtgccag cgcaatcggc
    10801 aaaccgagtt gaatcagtcg tttcataacc gcaggctcgg gcttagcggt gcctttttcg
    10861 ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc aaggaacatg
    10921 acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc aggcataccg
    10981 aaatgaccat aaataaagat atagttcacc gggatgttca ccagcaggcc gataaagccc
    11041 attaccatac ccggcttggt ttttgccaga ccttcacatt ggttacgggc aacctggaag
    11101 aacagatatc ccggcgcgcc ccacaacaac gcacgcagat aacccacggc tttgtccgcc
    11161 agagccggat cgatgttttc catgtagcgg ataatgtaac ctgcattcca cagcaccagc
    11221 ataatgagaa cggaaacaaa acctgccagc cagaaacctt gtcgcacctg atgcgcaatg
    11281 cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc cagcagcagt
    11341 ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac cgccgccatg
    11401 tcggtggcac tatagccgcc cgccatcacg gtatcgacaa aacccatcgc agtttgggcg
    11461 atttgcgcga gaatcaccgg gattgctaat gctaataaca gacgcgcttc actgatatac
    11521 ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc cgcctgaatg
    11581 ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt tattaattct
    11641 ccagtgaaaa aatagccatt cttacccctt cactggtaag ggcgttttcc acctgctatt
    11701 gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac ggggattgta
    11761 cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgtac gcatgtggtg
    11821 gagttacccg accacatgct ggacggcctg gaaaccggtg cttccgtggc gcataacggt
    11881 tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct gatgaaagaa
    11941 acgttacgca ttaccaatct tggcgattta aaagtggggg attgggtaaa cgttgagcgt
    12001 gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat tatgaccacc
    12061 gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt taaagtccag
    12121 gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga cggtattagc
    12181 ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc ggaaacactg
    12241 gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga aatcgatcca
    12301 caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga aaatgccatg
    12361 aatcaaccag gtacagaagc ctgatctgaa ctcgcccccg aagagggggc gatagaatag
    12421 cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacca cctgccagag
    12481 ctgaatatca cgggcgcgga aagcacctgc gcaggcattc agataatagg taaacatccg
    12541 tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgagga atcgttcata
    12601 ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt ccatcacaaa
    12661 gtggggttcg ctggattgag caatctggcg tacagagggc aggcaaccgt tcggaaaaat
    12721 atatttatta atccagggat caacattcag atcggttttt ttcgaaccga tagtatggag
    12781 caggaatata ccttccggtt tcaaattacg atccaccacc gcaaaatagg tatcgtaatt
    12841 tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact ggtcgttcag
    12901 gtcacgataa tcttgcagca aaatggtgac atccaggcct ttacagcgtt cctgagccat
    12961 tttttgctgt tcggcagaaa tggtgacgcc gaccacgctt acgtcatagt tagatgccat
    13021 gtagtgcgct agtccgcccc agccgcagcc aatatccagt acgcgcatcc ctggttttaa
    13081 ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca gattatcggc
    13141 atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc tgaacaagtc
    13201 attacccaaa tcgtaatgct ccttgccgac tatccaggca cgttttttac tctgcagatt
    13261 gaagagacga gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga gttggttctc
    13321 gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt cccaccagcc
    13381 atccatataa ctttcgccta accccaaaga gccttcctgc agaacgcgtt taaaaaaatc
    13441 ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac cggcacggct
    13501 aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt cttctataca
    13561 cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa cagcgtagac
    13621 gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa tccgacgtag
    13681 aacagaggga aaggaggaac ccttgccgaa atgaccatcc atagtgagtc gggaacgatc
    13741 ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt agtataggcc
    13801 gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta tatcgatata
    13861 gatcagtgtg attcgctatg agcgacttcg gcattgccat gattcgggca gtcaacttct
    13921 tcacaacgtt gcatcatgta acccagcgcg accagcacta ctgttgataa catcacgctg
    13981 gtggtggtga gcaatggcgt gctgatacta ataagccagg aaactaccag acttgcgagg
    14041 aagcacagac ccagttgcag agtgttctgc aacgctgcgg cgcgaccggt tgcatgtggg
    14101 aagggacgca gcgcctgggc gacaacaata gggtagatcg cgccattggc aatcgccatc
    14161 acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc cgcagcccag
    14221 gtcgcaatga cgctgacagc aaacagcacc agcaaccacg gtaataactg cttgccttgc
    14281 catttctgca gtgcggcgcg acagccataa ccaccaatca gaaacgcgat agtttgcggg
    14341 acataactta aaccaataac tgccgggctg tagcccattt cactaaggat gaacggtgaa
    14401 ccggtcagcc atgcaaaaaa actggctgaa caggctgcgt atatcagcac gttgccgcga
    14461 taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt gttacgggcc
    14521 ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat ggcaaacagg
    14581 gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa caggggagcc
    14641 agtgccggag acagacccac cagcggcatg atggtcgcaa aaatacggtt gactttctgt
    14701 gaaggataat aatctgtcac taatgcttgc cagataaccg ccgcggcgca gacccccaca
    14761 gcctgtacaa aacgcaatac cagcagcgtg gcggcgtttt ctacccacag catccccaga
    14821 ctacctaacg caaaaattgt caggccgatt aataacaccg gtttacgacc ataacggtcg
    14881 gagagtggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag actaaggctg
    14941 gcactgacag cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa agcaggcaga
    15001 tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac taaaaatctt
    15061 ttcccaggtt gcatgtatat tctctaaatc tctcaattaa acaccgccgc aaagtgtagg
    15121 gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa attttgcagg
    15181 cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg taatggtagt
    15241 tttagcgctg cggcacagga gctgcatcgt gttccttctg cggtcagcta taccgtgcgt
    15301 cagctggaag agtggctggc ggtaccgctc tttgaacgac ggcaccgtga tgtggagctg
    15361 accgctgctg gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa aatgcagatc
    15421 acccgccagc aatgtcagca gatagcgaac ggctggcgcg gtcagttagc tatcgcagtg
    15481 gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta tcgccatttt
    15541 gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga tgcgctttcc
    15601 gacgggcgcg tggaactggc aattggcgca acacgcgcga ttccggtagg cggtcgttat
    15661 gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagtca ccacccgctg
    15721 gcgttgatgg atggcccgtt cagcgatgat acgttgcgca actggccgtc gttggtgcgc
    15781 gaagacacct cgcgaacgct acctaaacgt attacctggt tgctggataa ccaaaaaaga
    15841 cttgtcgtac ctgactggga atcatcggca acctgtatct cggcaggatt atgcataggg
    15901 atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt agcacttgaa
    15961 ctggaaaacc ccttcccgga ttcggcatgt tgtctgacat ggcagcaaaa tgatatgtcg
    16021 cctgcgttaa cctggctgct tgagtatctc agcgatagtg agacgctgaa taaggagtgg
    16081 ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc gcggaacggg
    16141 ccgtcagcca cggagcggcg ttcaatcaaa cgcggatgca cttcaatgga ctgcggttct
    16201 tcacgtttgt tgacgatacg atccaacagc atgttgaacg ctgtttcacc cagcgaatct
    16261 tttggctgat ggatcgtggt cagcgccggc gtaaaatagc gcgcgttgcg cacgttatca
    16321 taaccgatca gcgaaacatc ctgcgggacg cgcaggccca tttcatcagc agcacaaagt
    16381 gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg cggctgcgac
    16441 aggatttgct gcatggcgcg ataaccggat tcaggttcaa agtcaccctg cacaatccag
    16501 ctttccggca ccttgatcat cgcttcttcc atcgccttca taaaaccggc aaggcggcct
    16561 gcgccggtgt tacgttccag cgggccgggg atgacgccga tttcgcggtg accgcgttca
    16621 atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac cgcatcggtg
    16681 aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg atactcttcc
    16741 agcatcgcca gcaacggctc tgggtactca gaacacatca ccagcagacc atcgacgcgt
    16801 ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattgttcca cgcattaccc
    16861 agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat gatctcggca
    16921 aaataggccg cttcgctgct ggtcgccagc aaaccgatag acttggtgtg gttaaccttc
    16981 aggctacgcg ccaccgcgct aggggagtag tgtaattctt taatcgctgc ccacacggcg
    17041 ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac agttgtagtg
    17101 gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca ctccagaccc
    17161 taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc ttacgcacaa
    17221 caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg tcaatggcca
    17281 gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg gaagtggtaa
    17341 aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa aaatgcgtaa
    17401 tatcactttt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga ccttaaattt
    17461 tcactggtaa caacggttat cgtcctcggt ttgatcgtag ccgtgggttt gactgccgcg
    17521 ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc tgtttgttag
    17581 caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat tgctgtcatg
    17641 ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata aggcatgaaa
    17701 attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac aacggagttc
    17761 tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat tcccgctgcc
    17821 ggaatgttaa tcagtgccta tgcagttggc gtaatggtgg gcgcgccgct gatgacgctt
    17881 ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat tttcacgcta
    17941 ggcaacgttc tttccgccat cgcgccggat tatatgaccc tgatgctttc acgtattttg
    18001 accagcctga atcacggagc attttttggt ttgggttcag tcgtggccgc aagcgtggtg
    18061 ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac cctggcaaat
    18121 atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg gatgtcattt
    18181 ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc attacctaaa
    18241 ggtggtacag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat gcgtccgcag
    18301 gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac cctctacacc
    18361 tatatctctc cggtactgca aagtattacc cacgcaacac cggtgttcgt cacggcaatg
    18421 ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa actggcagat
    18481 cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat tatgctggca
    18541 atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt gtggggcgca
    18601 gcaacctttg cggttgtacc tccgttacag atgcgcgtga tgcatgtcgc cagtgaagcg
    18661 ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc gctgggagca
    18721 gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc ggtgacgggg
    18781 gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa acaacctgaa
    18841 acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc ttctgcgtta
    18901 cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct catccgcgtt
    18961 ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat tatgcagcga
    19021 gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac
    19081 gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag
    19141 tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat
    19201 cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa
    19261 actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc
    19321 cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga
    19381 cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt
    19441 taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatgat
    19501 gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga gcatctttag
    19561 catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat
    19621 acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag
    19681 atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc
    19741 attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt
    19801 tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg
    19861 acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagc
    19921 tcactacgtt cgataggtcc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga
    19981 atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg
    20041 gtacgcggtg agctgccgcc ccaacgatat ggcttaccaa tttgctgcat cagtttattc
    20101 attgccactt tcgtggcttt ttgcaccttc gctttgtgcg cgtcggcaag ggttcctgac
    20161 gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg
    20221 gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta
    20281 gaagcggtaa tggaagattt tttaacagaa gaagcggttt tgcttttggt catactttta
    20341 ctggcggctt ttttcgctgt tttctggctg gctttctggg tctttttggt ggttgccgtg
    20401 cttttctttt tatctgcttt tttggtgata tgagtggtag cagaactctc cctggcttgc
    20461 tttgaagcat gggccatagg cgttaaaggc agggtggtaa aaagtaaagc acagagcgtg
    20521 atcgaaatac ggtttatccg cgccactgag cgtccctctg ttaaggcagg tctatcatgc
    20581 ctgcgtctga tttacaaaac ttgatgattc taatgcatag aagaagagaa agttaatagc
    20641 ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca gtgaaagcta
    20701 aaaatggtta taaatacgcc agtttgccgc gcatcagaga agaaacgaat atttgaaatg
    20761 caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg
    20821 ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca
    20881 atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac
    20941 atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt
    21001 gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg
    21061 gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg
    21121 gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc
    21181 aaagaaactg ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa
    21241 tactgacgga tgcgaacggg catccgtcag tcctttatcc ggtgcgccgt aaaacctcgt
    21301 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    21361 atgtactggc ggatgacgga tattggcgca ccttcgcagc tactggcaaa atagccagga
    21421 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    21481 aacctattcg atatgccttt gaggctgttt accagactgg atatcgccag tttgggagga
    21541 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagctgca
    21601 aaatcagcac atacatttga aaagtaagtg cgtagttttt ctgtcgtgtc atggtcaaaa
    21661 atctggcgtc ggtatttggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    21721 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    21781 cgattacagg catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg
    21841 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat
    21901 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    21961 aaaaacgcca ctgaaacgca atggcttaaa gattcgccct cacagccatt gcaacagtca
    22021 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccaga agcgggctgc ttttccccga
    22081 ttcaaaaagc ggggacagaa tgctgcattc cgctacccgc aggacgttaa gctcgatcag
    22141 gaaaacagcc gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag
    22201 gtcacgggag ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    22261 attcagacag aaagtgaagt atcaactccg gttcaccctt cagcatcaat gatcgggctg
    22321 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    22381 agttttcaaa aaaaccagaa gacgctggcg agacttcagc gacagttaag ccg
//
LOCUS       DAJTNP010000061        22283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTNP010000061 DAJTNP010000000
VERSION     DAJTNP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1030)
                     /locus_tag="OO160_002561"
     CDS             complement(<1..1030)
                     /locus_tag="OO160_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524630.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAK"
     gene            complement(1132..2112)
                     /locus_tag="OO160_002562"
     CDS             complement(1132..2112)
                     /locus_tag="OO160_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5524631.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2120..2830)
                     /locus_tag="OO160_002563"
     CDS             complement(2120..2830)
                     /locus_tag="OO160_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5524632.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3047..4870)
                     /gene="typA"
                     /locus_tag="OO160_002564"
     CDS             complement(3047..4870)
                     /gene="typA"
                     /locus_tag="OO160_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5524633.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5243..6652
                     /gene="glnA"
                     /locus_tag="OO160_002565"
     CDS             5243..6652
                     /gene="glnA"
                     /locus_tag="OO160_002565"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5524634.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6825..7874
                     /gene="glnL"
                     /locus_tag="OO160_002566"
     CDS             6825..7874
                     /gene="glnL"
                     /locus_tag="OO160_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5524635.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7886..9295
                     /gene="glnG"
                     /locus_tag="OO160_002567"
     CDS             7886..9295
                     /gene="glnG"
                     /locus_tag="OO160_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5524636.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9407..9517
                     /locus_tag="OO160_002568"
     CDS             9407..9517
                     /locus_tag="OO160_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5524637.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9746..11119)
                     /gene="hemN"
                     /locus_tag="OO160_002569"
     CDS             complement(9746..11119)
                     /gene="hemN"
                     /locus_tag="OO160_002569"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5524638.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11308..11817)
                     /gene="yihI"
                     /locus_tag="OO160_002570"
     CDS             complement(11308..11817)
                     /gene="yihI"
                     /locus_tag="OO160_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5524639.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12399..13031
                     /locus_tag="OO160_002571"
     CDS             12399..13031
                     /locus_tag="OO160_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5524640.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13413..16199)
                     /gene="polA"
                     /locus_tag="OO160_002572"
     CDS             complement(13413..16199)
                     /gene="polA"
                     /locus_tag="OO160_002572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5524641.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16563..17495
                     /locus_tag="OO160_002573"
     CDS             16563..17495
                     /locus_tag="OO160_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5524642.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17536..18966)
                     /locus_tag="OO160_002574"
     CDS             complement(17536..18966)
                     /locus_tag="OO160_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5524643.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19121..19747)
                     /gene="dsbA"
                     /locus_tag="OO160_002575"
     CDS             complement(19121..19747)
                     /gene="dsbA"
                     /locus_tag="OO160_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5524644.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19764..20750)
                     /gene="srkA"
                     /locus_tag="OO160_002576"
     CDS             complement(19764..20750)
                     /gene="srkA"
                     /locus_tag="OO160_002576"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5524645.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20827..21096)
                     /locus_tag="OO160_002577"
     CDS             complement(20827..21096)
                     /locus_tag="OO160_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5524646.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21166..21750
                     /gene="mobA"
                     /locus_tag="OO160_002578"
     CDS             21166..21750
                     /gene="mobA"
                     /locus_tag="OO160_002578"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5524647.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21732..22259
                     /gene="mobB"
                     /locus_tag="OO160_002579"
     CDS             21732..22259
                     /gene="mobB"
                     /locus_tag="OO160_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5524648.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5513 a         5584 c         5333 g         5853 t
ORIGIN      
        1 tttttgcttc gccgataggt gcaaaaaata cggctcgctg tgtaaagact atcgcgccaa
       61 agcccagcgt acatgccatc cctaaataga ccggcatact ttcgtgcggc agcataatca
      121 acagtacgag cgcagcggta ctgatgataa aggtgtagca tagatattta ctcggcgatt
      181 tcaggatctt atctgaaatc atgccaccaa tcggtccacc aatcattttc agacagtatt
      241 ggttaatgat gccgtaagcc cccaccagcg caacgggcaa tagatagatg tttttcagga
      301 atggaatgaa gaatgtcagg ccacagtaaa ccgcgtaaac gaagaagacg ttgaaagcga
      361 taagccagat ggttttgtct ctcagcactg aggtcatact ggtgttttgc gatgctccgt
      421 cttctttttt cacctcaacg gacggtgcct cttctttgtc attcaggaca aagaaaataa
      481 taatgcctac ggcaatcaca ataagggaat agaaccagat gcctgcttta aaacctaata
      541 agccactgcc aaaccaggta aatactgcca acgcagaaaa tgccaccacg gtatcgacaa
      601 tgccacgccc tgtttcgaag aagccaaaca accgaccttg ttgttcgcta ttgcccaatc
      661 gacttaccga cttgagcaag accggccagt tcatcatgtc gcaagtaacg ccaaatagcg
      721 cccagacaaa gaggatgccc caataccccg gcattgtcgt caaataaaca cccagtaatc
      781 ctgtcgcaat gagtgagaat gacatggtgt atctgcgcgg tagtttatcg gcaaaataaa
      841 tagacagaaa aaagcccact gtggtgacaa atgagtttac cgacatagca ttaccaattt
      901 gaccattggt caaatggaaa tattcctgca tcgggatata aaacgcatct ttcagcgacg
      961 gtaatttata aattgtaccg ccacacagtg ttaatagact aaataacgcc catttctttt
     1021 tcgtgagcat aacatcccct aagcgatttt taaaatcact gttttaaagg caataagata
     1081 ccgaccattc atatggaatg gttttattaa ctgcgtatga gttaaaactt attagtggtt
     1141 cagaagatgg tttgcctctt gtttgatttg ttgtagtacg ttacgcacat gatttatctg
     1201 attaattgca tcttcttttt ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag
     1261 gggtgtttca ctcatctggc gataagggat ccacggatta tcatcttcgt cttcaaagcg
     1321 gaacgccgac gcatagtaat caacctcttc ttccaggcca tatgccgtca cctgcggctc
     1381 atcatcgcca aggcagataa ggtgcgttaa taacgctttg aatggcatat caccctgacc
     1441 tgaaatacag gctttatgac ccaggccacc ctgttcttta acgatcaagg catctttaat
     1501 atggacctgg gtaatatgcg gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc
     1561 attgatcatg ttagcaaaat caaacagtaa ggaaagagac tccatctcac tttctttgac
     1621 cagcgacacc agctcatgac tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc
     1681 gctgtcctga tacgtttccc gtacataggc aatgtcgtta gcgataatcg ataatacgtc
     1741 gcgcaaatta ccttcataac atggataaaa acgtacggac gttgccccag ttttcaacgc
     1801 gatggcgacg gcttcgtcaa tagatgcctt atctgaagcg ctggtttcaa tatgaatatc
     1861 aaggttcaga cggcgggctt tatccccaaa ggcagagagt tctttatcgt ccatattacc
     1921 aagagaaaaa cgctcgccat ccagaacatg gattttgacg ccacgtagat tattttcact
     1981 tgcgatatca agtaaatcag caggcaacac tctttccagc cgcatattta aatgaaaggc
     2041 ataagcatgt aaataaagag gaagagtgtc gacacgttgt agaatctttc ttgcgttatt
     2101 tattgtcacc ataaaacacc tacagattaa tgatgaggtc tacggcctca aatcgccaat
     2161 attcaatatc tatctggaca atattgcctt gctggtcggc gcgatgcttt tcaattaaga
     2221 tcgctggcat acccgcagaa ccgccaagat acttgctgat atcgcccggc attctgacgg
     2281 gtttgaaaat caattttttt actaccgtct ctttttggta gcacttttcc cagactgcag
     2341 aaaatgagtg gttttcaagt tgttcaataa aacccggagc aacttcagga ttaatatatg
     2401 tttcgtgata gaaaacttta tgtccttcca gcgcccccca gccggtgatg cgatagagtt
     2461 cagttgatgg cgtcactgca atcagtggcg cgagcgtctc gggaatatcg ctggtacggt
     2521 ttttctcggt aaacccccaa gaaggctctc gcccttgctc aattgcggcc cgctgaaagc
     2581 tcgccgacag ctccggactg taattaaatc gcggctgggt cacgaaccac cccttacgat
     2641 ccttacggaa gattttggat tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac
     2701 gtttaatgcc cagcagttct cccagctctc tttctgaagg gagttttcca cccggcgtgg
     2761 tgatgccatc tttcaaccac cgatccagtt tctcttttgc attttctacg gtggattgat
     2821 tctccgccat tgtttaatcc ttttggttta aaccagatga agcatcttac gcttacgcca
     2881 aaaaagaaag caattaacgg tgtatgactt tgcagataat tgtgacaaag ctctctattg
     2941 acgtaatcac agacttataa gggagatact cgcattggtg tgcgctcacg acaggtaaaa
     3001 aaaaacctgc catcgctggc aggtttttta tgactaaaaa acgaaattaa tcgtctttcg
     3061 gtgcgcggtt ggcgcggcga cgatcgtttt ccgtcaggtg acgtttacga atacggatag
     3121 aggtcggagt cacttctacc agttcgtcat catcgatgaa ctccagagct tgttccagag
     3181 tcatgcggat aggcggaacc agaacaacag cttcgtcagt accggaagca cgcatgttgg
     3241 tcagtttctt accggtcagg cagtttacag tcaggtcgtt agagcggcta tgaataccga
     3301 taatctgacc ttcgtaaact tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca
     3361 gaccgaacag cgcgaacgcg acagctttac cctgaccgtt agagatcagt acaccgttct
     3421 gacgctgacc cacttcgccc ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca
     3481 gaccagtacc ggaagtcatg gtcatgaact cagaacggaa gccaatcaga ccacggcttg
     3541 ggatcacgta gtcgagacgt acgcggcctt taccgtctgg attcatgttt ttcaggtcgc
     3601 ctttacgttc gcccagcgcc tgcatcacag aaccctgatg ctgttcttca acgtccagcg
     3661 tcacgttttc atacggctct tgtttacgac cgtctatttc acggaagatt actttcggac
     3721 gggataccgc cagttcgaaa ccttcacgac gcatgttttc gataagaaca gagaggtgca
     3781 gttcaccacg accagaaacg cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg
     3841 caacgttgtg taccagttct ttgttcagac gatcgaggat ctgacgagac gttacgaact
     3901 taccttcttt accgcagaac ggcgaggtgt taacgcagaa gaacatagaa acggtcggct
     3961 catcaacgga gagtgccggc agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa
     4021 tgttcagttc gcccaggccc gtgatcgcca cgatatcgcc agcttccgcc agatcggttt
     4081 cgatacgttc cagaccgagg tggcccagca ctttaccgac tttcgcgtta cgggttttgc
     4141 cttcgctatc gatgatagta acctgctggt tcggcttcac tttaccacgc ttgatgcggc
     4201 caatgccgat aacgccaaca tagctgttgt aatcgagctg agaaatctgc atctggaacg
     4261 gaccgtcaag gtcaacgtcc ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg
     4321 tcatgtcttc cgccatatct tcgtggtcca gacccgcgat accgttcagc gcagaagcgt
     4381 aaacgatcgg gaagtccagc tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata
     4441 cctgatccac aacccaatca ggacgcgcgc cagggcggtc aactttgttg ataacaacaa
     4501 tcggcttcag gccgtaagca aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc
     4561 cgtcaaatgc gtcaaccacc agcagcactg agtctaccat ggacattaca cgttcaactt
     4621 caccaccgaa gtcggcgtgc cccggggtat caacgatgtt gatacggtaa tcattccatt
     4681 tgatagcggt gtttttcgcg aggatggtaa tcccacgctc tttctccaaa tcgttggagt
     4741 ccatcacgcg ctcttgggtt tcggcacgag agtcgaacgt accggattgt tggagcagct
     4801 tgtctaccag ggtggtttta ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt
     4861 tttcgatcac aactttgcct caggcattag aaatagcgcg ttattgtaca cggattaatc
     4921 gcactacaaa acaggatcac aaacatcctc cgcaaacaag tattgcagag tccctttgtg
     4981 atcgctttca cggagcataa aaagggttat ccaaaggtca ttgcaccaat atggtgctta
     5041 atgtttccat tgaagcacta tattggtgca acattcacat catggtgcag cccttttgca
     5101 cgatgttaag catgataacg ccttttaggg gcaatttaaa agttggcaca gatttcgctt
     5161 tatctttttt acggcgacac ggccaaaata attgcagatt tcgttaccac gacgacaatg
     5221 accaatccag gagagttaaa gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca
     5281 cgaagtgaag tttgttgatt tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac
     5341 tatccctgct catcaggtga atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc
     5401 ctcgattggc ggctggaaag gcattaacga atccgacatg gtgctgatgc cagacgcatc
     5461 cactgcagtg attgacccgt tcttcgccga ctccaccctg attatccgtt gcgacatcct
     5521 tgaacctggc accctgcaag gctatgaccg tgacccgcgc tctattgcga agcgcgccga
     5581 agattacctg cgttccactg gcattgccga caccgtactg ttcgggccag aacctgaatt
     5641 cttcctgttc gatgacatcc gtttcggatc atctatctcc ggttcccacg ttgctatcga
     5701 cgatatcgaa ggcgcatgga actcctccac ccaatacgaa ggtggtaaca aaggtcaccg
     5761 tccggcagtg aaaggcggtt acttcccggt tccgccggta gactctgctc aggatattcg
     5821 ttctgaaatg tgtctggtga tggaacagat gggtctggtg gttgaagccc atcaccacga
     5881 agtagcgact gctggtcaga acgaagtggc tacccgcttc aataccatga ccaaaaaagc
     5941 tgacgaaatt cagatctaca aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac
     6001 cgcgaccttt atgccaaaac cgatgttcgg tgataacggc tccggtatgc actgccacat
     6061 gtctctgtct aaaaacggcg ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga
     6121 gcaggcgctg tactacattg gcggcgtaat caaacacgct aaagcgatta acgccctggc
     6181 aaacccgacc accaactctt ataagcgtct ggtcccgggc tacgaagcac cggtaatgct
     6241 ggcttactct gcgcgtaacc gttctgcgtc tatccgtatt ccggtggttt cttctccgaa
     6301 agcacgtcgt atcgaagtac gtttcccaga tccggcagct aacccgtacc tgtgctttgc
     6361 tgccctgctg atggccggtc ttgatggtat caagaacaag atccatccgg gcgaagccat
     6421 ggacaaaaac ctgtatgacc tgccgccaga agaagcgaaa gagatcccac aggttgcagg
     6481 ctctctggaa gaagcactga acgaactgga tctggaccgc gagttcctga aagccggtgg
     6541 cgtgttcacg gatgaagcga tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg
     6601 cgtgcgtatg actccgcatc cggtagagtt tgagctgtac tacagcgtct aatagttgaa
     6661 gttgtactac ccggcgcaca acgccgggat ttagttgccg tggaaacttt cagcccatct
     6721 ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag
     6781 ctataatgca ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc
     6841 agcccgatgc tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca
     6901 atctggcgat ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca
     6961 aattgtttgg tacgccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga
     7021 tgcaagaaag tctggaggcg gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg
     7081 atggtcgctc gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc
     7141 tggagatggc accgatggat aaccagcgcc gcttaagcca ggaacaacta caacatgctc
     7201 aacaagtagc tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg
     7261 gcggtttacg tggcgcggcg cagttactca gcaaagcgtt acctgaccca tcactactcg
     7321 aatataccaa agtgattatc gaacaggcgg accggctgcg aaatttggtc gaccgtctgt
     7381 tggggccgca gctgcccggt acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg
     7441 tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgatc
     7501 ccagcctgcc ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg
     7561 tgcgcaatgc gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca
     7621 ccgcgtttca actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg
     7681 aagataacgg gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca
     7741 gcggccgcga aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc
     7801 attcaggcaa aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc
     7861 ctatcaggaa ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga
     7921 tagttccatc cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac
     7981 atttgagaac ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct
     8041 ttcagatatc cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg
     8101 ccatccaatg cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag
     8161 cgcctatcaa caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt
     8221 ggcgctggtt gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca
     8281 gcttaacggc ccaacgaccg atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg
     8341 tattatcggt cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac
     8401 cggtaaagaa ctggtcgctc atgccctgca tcgccacagt ccgcgcacca aagcgccgtt
     8461 tatcgcgctg aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca
     8521 cgagaaaggc gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga
     8581 tggcggcacg ttatttctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt
     8641 gctgcgcgtg ctggcagacg gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt
     8701 ggatgtgcgg attatcgctg ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa
     8761 gttccgtgag gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg
     8821 cgaacgtcgg gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga
     8881 actgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc
     8941 gtggccaggc aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc
     9001 cgggcaggaa gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga
     9061 gagtacttcg caaatgcaac cggacagttg ggcgacactt ttagcgcagt gggcagacag
     9121 agcgctgcgt tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac
     9181 gttactgacg accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact
     9241 cggctggggc cgcaacactc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac
     9301 ggcttgtgtg taaagattga ttattgagcg caaattgctg gtattttgcg ctttactgtt
     9361 ccgataagtt cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat
     9421 aattaatctg gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc
     9481 cgtgctgtgt gccgcgatga ttgggctgtt tagctgaagc tcgttgttta tgccggatgc
     9541 ggagaaagtc gtaggcctga taagacgcga ccagcgtcgc atcaggcatc atgctcagat
     9601 gcctgatgcg gcgtaaacgc cttatccggc ctacaaaagc aataatttca acaggttttg
     9661 ttgcccagcg catcaggcaa tcttgttctc agcacattga tggcgctttc gttttctact
     9721 ttgtaaacga agcgccattc actctttaaa tcacccgtga gaactgctgc atccgcgctt
     9781 tctggcgcag ataggtatca aagcacatgc aaatgttgcg gatcagcaag cgacctttcg
     9841 ccgtcacctg tattcccttc tcatccacat ccaccagccc atcttttgct aacggggcga
     9901 gcagcttgag atcttccgca aagtaatcag cgaagtgcaa atcccactgt ttctcaagag
     9961 gggcgtaatc cagacggaag ttgcagatga gcgacttaat cacatcgcgg cgaatacagt
    10021 catcacgcgt tagcgcaata ccacgccaca gcgcattgcc ttgttcatac acttgctgat
    10081 agtactgctt caactctttc tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg
    10141 aaacgcccat ccccagcaga tcggtatcgc cctgagtggt gtagccctgg aagttacgat
    10201 gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc aggacgggca aagtgatcca
    10261 taccgataaa ctgatagccc gattgcgtca ggaaggcgat ggtttcctgc aggatatcga
    10321 gtttttgctg cggactcggc aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg
    10381 tcggcagatg cgcgtagtta aaaacgctca gacgatcggg gttcagctcc gccacacgtt
    10441 tcagggtaaa ggcgaaactc tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt
    10501 tggtggaggt aaaaccaatc tcacgcgcat ggttaagcag tgcaaagatg aactcttcat
    10561 cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa gtcctgcacg cccatgctca
    10621 ggcgattaaa gccttcggcg cgtaaatgat cgagtacatc cagttcgatt tcccgcggat
    10681 cgacttcgat cgaaatctcc gcatcggtat tgaaatggaa gttttcgcgc agcagcttca
    10741 tcagacggct gatttgcgct ttattcagat acgtcggcgt tccgccgccc cagtgcaatt
    10801 ggctgacgtg acgcccggcg aacagcggtg cacgatggac gatttcttgc tccagcgcgt
    10861 ccagatactg atcggccttg tgctgctggc gagtaacaat cttattgcaa ccgcagaagt
    10921 aacaaagctt atggcagaac gggatatgta cgtagagaga taatggacgc tcaggatagc
    10981 gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg
    11041 ggtacgaggt gtatcgtggc ccggaatagt tatatttctg gatcagggcc agatcccagt
    11101 cgatttgctg tacagacatg ctcactcctt ccgatggcgt ctctggcggc tacggcgtac
    11161 cggcggatgc ccgtttcggg tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa
    11221 ccgctgtagt ttaacgaata accacaccag ataacatata accggaaggg ttatcagcag
    11281 aacgggaagg cccacggcgg caatccgtta gttgccccgc agcagacgca tcatgtcttc
    11341 ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa gagaggccga gtttctgcat
    11401 caactcatca atacgatcca gtttagcatc cacccaggat tgctcttcgg cgctcagggt
    11461 ttcgcctgct tccagacgtt ccagcagcgc atccagacgc tcatccgttt ccagtaattc
    11521 caactccgcc tgcggtgaaa gcataggttt ctcactcttc ggtttgtgct gtttggtgac
    11581 tttttcagtc actcccaatg gaataggggt tttactgcca atacgtggat cttttggtgc
    11641 gttctggcct ttgctgcctg acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg
    11701 accacgacgt tttttctgac gcttacggtc acgagcttcc tgatccagct cctcgcgtgt
    11761 ttttcgacgc gcttttgcat gacctttgct gcgtgagttt gaagatgatg gtttcataat
    11821 ggttcgtctt ttagccgttt tattcagtat agatttgcgg cggaatctaa cagaaagcaa
    11881 gcaaagaaaa aaggcgacag attactctgt cgcctttttt cctgactcat aacccttaac
    11941 gggtcttaca atccttgcag gccgccaaca caccctgttt ttccattagc cggaacaatc
    12001 cgtgttgatt ccatttccgt ttaattacgt ctccggacgt ttgtcttcct gacaatcctc
    12061 tgtcttcgcc tcctggcgct cctgaccctc atcctgagtc attgttcctg ttagcgtcct
    12121 cgctctatgg gtgctacttt acgcctttgc tttaaacaaa caatcaaccg ccatcagtat
    12181 ttgcacattt tcagagtttt atataaaagc aattcactga tttatatagc ttatttgaca
    12241 tgcatcgcag aattttctct gctatttcgc ataaagacta tcggcaatgt ctcacaaagc
    12301 gtatggcttt tacttacaac gctcacgccg aaaaatccgc cttttattat ggttcaggta
    12361 taatcgccac aaagctttga ctgacggaga cgaccgcttt gactaatttg aattatcaac
    12421 agacgcattt tgtgatgagt gcgcctgata ttcgccacct accttccgat accggaattg
    12481 aagtggcttt tgcaggccgt tccaacgcag gtaaatccag cgcgctgaac acgctgacta
    12541 accagaaaag cctggctcgt acctcaaaaa ccccagggcg cacccagctt atcaacctgt
    12601 ttgaagtggc tgacggcaag cgtctggttg acttgcctgg gtacggttat gcggaagtcc
    12661 cggaagagat gaagcgcaaa tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga
    12721 gcctgcaagg tctggtggtg ctaatggata ttcgccatcc gctgaaagat ttggatcagc
    12781 agatgattga gtgggcggta gacagcaata tcgccgttct ggtgctgctg accaaagcgg
    12841 acaaactggc aagcggcgca cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg
    12901 cgtttaacgg tgatgtgcag gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca
    12961 agctgaggca gaaactggat acctggttta gcgagatgca gcctgtagaa gaaacgcagg
    13021 acggcgaata attttcttgc cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa
    13081 ttcaatagat tacaattcta ccgttggcct gataaacata gcgcatcagg cattacggat
    13141 cttttctttc gcccaataaa aaacgcccca gtcattactg actggggcgg ctaaaatatt
    13201 cagccaaatc cgattacgtg aagtaaaagg tctgaaagat agaacatctt acctctgtac
    13261 cctacgcgac taactttact cttttttgtt cagttcagaa agcacttttt gtaatttttt
    13321 ttcagttgtt gcataggaat ttctaatagc catcacaaaa cgtgacagct tatgttgctt
    13381 acttacgaaa aaagggcatg ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca
    13441 ctccccactt ccaccagcaa cggcacatcc agacgggtac agttttccat cagttgatga
    13501 atctgcttcg cgacggcatc aacatcatct ttatgaactt caaataccag ttcatcgtgt
    13561 acctgcatga tcatacgtac acgcggttgc tcagcctgta accacgcatc aacggcaatc
    13621 atcgcccgtt tgataatgtc ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt
    13681 tcagccgctg cacgacgagc accattgctg gatttgatat ccggcagata cagacggcgt
    13741 ccgtccagcg tttcaacgta gcccagctct ttcgcctgag cacgggtgcg ttccatatac
    13801 tccagcacgc cagggtagcg ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt
    13861 ggaatgttca attgccgcgc cagaccgaaa gcactcatgc cataaatcag accaaagttg
    13921 atcgctttcg cgctacggcg ttgctcgctg gtgacggttt ccagtggcaa accaaacact
    13981 tctgccgccg ttgcccggtg gatatctttt ccttccgcga atgcggtcag caagccttta
    14041 tcacgcgaga gatgcgccat aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc
    14101 acataatcct ctggcgcaat aaacgcctgg cggatacgac gaccttcttc gttacgcacc
    14161 ggaatgtttt gcaggttagg atcggttgac gataaacgtc ccgttgcagt tactgcctgg
    14221 tgataagagg tatgcacacg cccggttttc gggttgatca tcagcggcag cttgtcggtg
    14281 taagtcgact tcagcttcgc cagaccacga tactccagaa tcacttttgg caacggatag
    14341 tccagcgcca gttcttccag tacctcttcc gacgttgacg gcgcgccacc cggcgttttc
    14401 ttcagcggtt taataccctg tttttcaaac agaatggttt gcaactgctt ggtggaagaa
    14461 aggttaaatt cttcacctgc aatttcatgc gctttctttt ccagctcagc cagacgcaag
    14521 gtgagctctt cagaatgatt gtgcagcact ttcggatcga tcttcacacc gttacgttca
    14581 atgcgtgaaa gcaccggcac cagcggcatt tcgatattct cgaagacgtt caacggccct
    14641 ttgtgtttct gcagatccgg ccacattttc agatgcaact gcaaggtgac atctgcatct
    14701 tcggcggcgt aacgtccggc ctcttcgagg gcaatctggt taaaggtcag ttgatttttg
    14761 cctttacccg caatctcttc aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg
    14821 ctgtccatat cgtgacgccc ggcaacgcta ttgagaatgt aggactccag catggtatca
    14881 aacgcaatcc cacgcagttc aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg
    14941 ttttgcccga ccttcagcgc cttttcatct tccagcagcg gttttagcaa ctcgagtgca
    15001 cgctcgtgag agatttgatc gggcgcatca agataatcat gagcaaccgg aatatatgcc
    15061 gctacgcctg gctcgatagc aaaagaaagc ccgaccaggt tagcagagat gttatcaagg
    15121 ctgtcggttt cggtatcaaa tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct
    15181 ttcagtgttt cttcatcaag gatagtgacg tagttgtcat aagaaatcac cgttgccgtc
    15241 acttctggcg cttcgtctgc aacactggtt tcctgcggct tcgcggctgt ttttgcccct
    15301 ttggcctgta accatttgcc cacttcgaca tcagcagtcc agcgtttgaa ctcatacttt
    15361 ttgaacagcc ccaacaactc ttccgctgcc ggttgctgca cttccagttg ttcacaggtc
    15421 agctccagtt caacatcggt tttaatcgtc gccaactggt atgagagata agcaacttct
    15481 ttgttttgct cgagcttcgc tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt
    15541 ttttctggct cggcatacag cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg
    15601 gttttttcac cgacgcccgg tacgccagga atgttatcag aggagtcacc catcagcgcc
    15661 aggaagtcga tgatcaattc tggcggcacg ccgtacttat tcaccacctc ttccggtccg
    15721 aggatggtat tcgtcatggt attgataagc gtaatatttg gcgtcaccag ctgcgccata
    15781 tctttatcgc cagtgctaat cagcaccgga cgcccggctt tttcggcttc gcgcgccaga
    15841 gtaccgataa cgtcgtccgc ttctacgcca gaaaccgcca gcagcggcag tcccatcgct
    15901 ttaaccatcg cgtgcagagg ttcgatttgt gctcgcagat cgtccggcat tggcgggcga
    15961 tgtgatttgt aatgttcaaa cagttcatca cgaaaggttt ttcccttggc gtcaaagacc
    16021 accgctgcat gcgtcggttt atattgcatg atcagactgc gcagcatgtt gaggacacca
    16081 tacatcgcac cggtcggctc gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg
    16141 cgataaagat aagatgaacc atctacaagg ataagtggat tttgggggat ctgaaccata
    16201 atgtccgtgc ctgtttatca gattatgggt aaaggatgcc acagacagga tgaaaacatg
    16261 agtttttcac cgcttttcgt gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt
    16321 catgtggata actttgtgca taaattattt acatcgcttt taatttatta tcatgctttt
    16381 tacagcatga tgtttgtctt tgtttttcat gcagttaaaa ataaatccat ccctaataat
    16441 tgagataatg ggattttttc taatgtggat agatatgaat tatttttctc cttaaggatc
    16501 atccgttatt tgggtcattt tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt
    16561 ctatggctaa ccttttgaat aaattcatta tgacgagaat actcgctgcg ataacccttt
    16621 tgctgagtat cgtattgacc attttggtca ctattttttg ttccgtcccg atcatcattg
    16681 ccgggattgt aaaactattg ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt
    16741 gtgattttat gatgtattgc tggtgtgaag gtctggcggt attactgcat ctgaacccac
    16801 acctgcaatg ggaagttcac gggctggaag ggttaagtaa gaagaactgg tatctgctta
    16861 tttgtaacca ccgtagttgg gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca
    16921 ttccgatgaa taaatatttc ctcaagcaac agctggcctg ggtgccattt cttggcctgg
    16981 cgtgctgggc gctggatatg ccgtttatga agcgttattc ccgcgcttat ttattacgtc
    17041 atccggagcg acgtggtcaa gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc
    17101 tgcatcccac caccattgtc aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc
    17161 agcaaaccca ctccactttt caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg
    17221 cgctaaatgt actgggtaaa caattcgata aactgcttaa tgttacgctg tgttatccgg
    17281 acaataaccg tcagccgttc ttcgatatgt taagcggtaa actgacgcgg attgtcgtac
    17341 atgtggattt acagcctatt gccgatgagt tacatggcga ctacatcaat gacaaaagct
    17401 ttaagcgcca ttttcagcaa tggctgaact cattgtggca ggaaaaagac cggcttctga
    17461 cgtctctcat gtcatcacag cgtcagaata agtaagtttt actcgcctct gaatgagcag
    17521 aggcgagtga gtattttaat gaacaaaacg tccggcacga gacataaatt cttcttcgct
    17581 catctcctgt ccgttaaaaa caactttacc cggtgtataa cgcagctgca gcgaggcggt
    17641 attgttgtca atcgtgatta actgaaacat ctgacctaat gtcatcatcc cgctgatttg
    17701 tttatcagcc tgcttttgcg ctttttccgc atccattcct tcagataaat taagctgttt
    17761 tgcggtttct gtgacgacat taagcggtaa cttcacatca aaattgagcg atttgatatc
    17821 tttgtttgtg gatgatgaag atttggctgg gtcggcaata ctgatatcca gattggcatt
    17881 tagttcgccg agtgcgttct tccatcttac cggttgttta atggtcggct cacttttttg
    17941 taataacggt aaatactctt tgaacaatgc agcattcact tcttgcagag caacttcatc
    18001 gtttgctaac tcagggtgtg cagcaagctg cttctgcatc gcaatgttat actggatgat
    18061 aaattggcgc actgctgagg gatcaataga ttcagcaatc aatgaccact caccactacc
    18121 gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga cattgatata
    18181 atctttgtct ttaacgcgat ccagcctggt ttttgcaacg aaagcatcga tttgggcaac
    18241 gtcttccccg tgattgataa tgttaatctg attaaatttc gactcgcttt caccaaccgg
    18301 aaatttcttt tcttctaatc gcgccagaga atcaatggta aaactttttg ccgttacttt
    18361 gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat ccaggttaga
    18421 caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa attttccata
    18481 ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt
    18541 cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca gttcattatt
    18601 cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca ttaacattgt
    18661 gatgggcaac ggaccgtgct caacatccac atcaaataca actttctggc ctggcttgat
    18721 attgagatcg ggtgcaccgt tatcgaatgt catttgcatt tgaaaacggg aattaaaaaa
    18781 gcctttgtca aagtttttat aacttaacgt catatcgtag gcatgttcac ctttcttttt
    18841 cgcatcgtta aaatctttaa taaacttttc gataccaggc tgaatttgcg tacctgtgta
    18901 ccatgtgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag ctgacttacg
    18961 tatcatataa atattccctg ataaaacagc aaaactttga atatccactt atgctgaaat
    19021 aatgtccagc ggcaggatgc attatcagaa tgtatttata tttattaaat aataaaaaaa
    19081 gcccgtgaat attcacgggc tttatgtaat ttacattgaa ttatttttcc tcggacagat
    19141 atttcaccgt atcagcatac tgctgaacaa aaacatccat attgctggta tccataccct
    19201 gcggattcag ctgatattta ccgttaacaa acatcgccgg aacaccacgc aattgcacat
    19261 cagctgcggc tttttcctgc tgagcgacca gagatttcac cacgaagctg ttccacgccg
    19321 cgtcgtactc ttcaccttta atacctgcat tgataaatac atcgcggata tcagatgctg
    19381 aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtcact ttgtcttcca
    19441 cgcccagcgc catcgccaca gcccatgcct gagtcagatc tttgcccagg tcaccaccca
    19501 tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc ttcacattat
    19561 cagaaatatg cagaacttct tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact
    19621 ccagcacttg cggcgcgcca gctaccggtt tttccagggt agtgtactgt ttaccatctt
    19681 catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc agccaaatct
    19741 ttttcatgat ctactctctc cgattaatac ataggtgtta attgcaaagg gggttcttgt
    19801 agaacttttg cctgttctat aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt
    19861 aaccacggga aatttttcgg gaacgcggga tcagcccaat gccgcattag ccaggcaaga
    19921 taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac
    19981 tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccgctg ctgggcttta
    20041 tcgccattga gcaacatcca caaatcctga atagctggac cattacgtgc atcatccaga
    20101 tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc tccatgtagc
    20161 cgcagaacgg tgaaatcttc ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc
    20221 gctttcagaa atgccgcttt caacccggaa ggtatcagtg tagcgtcctc aaacagcttg
    20281 cgaggctcaa tgagatattc attcagaccg atggtcgggc gatgaataaa aagctgtttg
    20341 cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg atcgatatta
    20401 tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa tccctgatga
    20461 ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc atcatttacc
    20521 agctgcaacg caaattgatg ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga
    20581 taaaatttga cgacaaaacg tcgacgctct tcgtcctgaa attgatagac acggttttca
    20641 tagctgttaa gcggggtaag accggaatcc acccggatcc cttgcttaaa cagagcgtcc
    20701 atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc
    20761 ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg cgccgcttac
    20821 aagcttttac tctttaatta cgccgcgagc gcgtaacagc gccgttttga agtcttcttc
    20881 ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg
    20941 atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg actctttcgc
    21001 cagtttctgc aaaaattgca acaagttaaa gtctggctct ttttgccagg ctggctggag
    21061 gagttcaata acttcattca gacgtttaca tttcatagta atgctcctta ctcttgagac
    21121 agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa tctaatgacg
    21181 acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggcgg cgtagataaa
    21241 ggattgctgg acttaaatgg caaaccgctg tggcaacatg tcgctgacgc gcttaagacg
    21301 cagctctctc acgtcgtggt taatgctaat cgtcatcagg aaatctatca ggcaggcggt
    21361 ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg aatgctttca
    21421 gtaatgcagc aggaagctgg tgagtggttt ttgttttgtc cgtgcgatac gccttacatt
    21481 ccccatgatt tagccgcccg gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc
    21541 catgacggtg aacgcgatca cccgactatt gctctggtaa accgcgctat tgagccttta
    21601 ttactggaat atctgcaagc aggagaacgc cgggtaatgg catttatgcg tctggctggc
    21661 ggtcatgcgg ttgatttcag cgatcataaa gatgcatttg ttaacgtgaa tacgccagag
    21721 gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc gcgtggagtg
    21781 gtaccggaaa aactacgctt ttgaaaaaac tgatcccggc attatgcgcc agagggatcc
    21841 gtccggggct gattaagcat acgcaccatg atatggatgt tgataagcca ggcaaagata
    21901 gctatgagct gcgcaaggct ggcgcggtac aaaccatcgt tgccagccag caacgatggg
    21961 ccttgatgac ggaaacacca gacgaagaag agctggatct acattttctc gcaagtcgaa
    22021 tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa
    22081 agattgtgct gtttcgcgat ggagccggac atcgacctga agaattagtg atagacaggc
    22141 atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcatta ctggatatta
    22201 atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag aacggataag
    22261 caagagtagg ctggatcaga cgc
//
LOCUS       DAJTNP010000062        21533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTNP010000062 DAJTNP010000000
VERSION     DAJTNP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO160_002580"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO160_002580"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5524649.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OO160_002581"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OO160_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5524650.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OO160_002582"
     CDS             complement(2650..3201)
                     /locus_tag="OO160_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5524651.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OO160_002583"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OO160_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5524652.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGLVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="OO160_002584"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="OO160_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO160_002585"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO160_002585"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5524653.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO160_002586"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO160_002586"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5524654.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO160_002587"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO160_002587"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5524655.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO160_002588"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO160_002588"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5524656.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO160_002589"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO160_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5524657.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="OO160_002590"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="OO160_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5524658.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="OO160_002591"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="OO160_002591"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5524659.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO160_002592"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO160_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5524660.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO160_002593"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO160_002593"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5524661.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO160_002594"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO160_002594"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5524662.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO160_002595"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO160_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5524663.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="OO160_002596"
     CDS             complement(18171..18566)
                     /locus_tag="OO160_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5524664.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO160_002597"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO160_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5524665.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="OO160_002598"
     CDS             19482..20075
                     /locus_tag="OO160_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5524666.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /gene="tabA"
                     /locus_tag="OO160_002599"
     CDS             20220..20672
                     /gene="tabA"
                     /locus_tag="OO160_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5524667.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21533
                     /locus_tag="OO160_002600"
     CDS             20795..>21533
                     /locus_tag="OO160_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524668.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5204 a         5325 c         5672 g         5332 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggcttggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaagttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctgatggtt cgactaatcg
    21241 ttatgaaggg aagtcttttg aaagaaaaga tgatagttca ttacacctga ttactaacaa
    21301 ggttctggcg tgttaccaac gcgaagctaa caagaaaata gcgcgtctat taaataatca
    21361 tcagaagtta aataatatac aggagttaaa tgattcgcag gagttaaatg attcgcagga
    21421 gttaaataat tcacagaagt taaataattc gcaggagtta aataactcgc aggtgagttg
    21481 taaaggttca gttgattcta cgattacgga tttattagaa aaagcattga ata
//
LOCUS       DAJTNP010000063        21496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTNP010000063 DAJTNP010000000
VERSION     DAJTNP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO160_002601"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO160_002601"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5524669.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO160_002602"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO160_002602"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5524670.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO160_002603"
     CDS             complement(3121..3420)
                     /locus_tag="OO160_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5524671.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO160_002604"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO160_002604"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5524672.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO160_002605"
     CDS             complement(4632..5069)
                     /locus_tag="OO160_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5524673.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO160_002606"
     CDS             complement(5115..5456)
                     /locus_tag="OO160_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524674.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO160_002607"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO160_002607"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5524675.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO160_002608"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO160_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO160_002609"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO160_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5524676.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO160_002610"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO160_002610"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5524677.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO160_002611"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO160_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5524678.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO160_002612"
     CDS             12687..13217
                     /locus_tag="OO160_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5524679.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO160_002613"
     CDS             13329..13865
                     /locus_tag="OO160_002613"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5524680.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO160_002614"
     CDS             complement(13973..14797)
                     /locus_tag="OO160_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5524681.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17146
                     /gene="malS"
                     /locus_tag="OO160_002615"
                     /pseudo
     CDS             15117..17146
                     /gene="malS"
                     /locus_tag="OO160_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="OO160_002616"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="OO160_002616"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5524682.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="OO160_002617"
     CDS             complement(18728..19201)
                     /locus_tag="OO160_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5524683.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OO160_002618"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OO160_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5524684.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20351..21343
                     /gene="yiaK"
                     /locus_tag="OO160_002619"
     CDS             20351..21343
                     /gene="yiaK"
                     /locus_tag="OO160_002619"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5524685.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21355..>21496
                     /locus_tag="OO160_002620"
     CDS             21355..>21496
                     /locus_tag="OO160_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5524686.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5400 a         5489 c         5276 g         5331 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgcgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggtgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccgga caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctgg ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gtaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgttctc
    20881 ttacggcatg ttagaagtta accgcctggc aggccgtcag ctcccggtcg atggtggctt
    20941 tgatgatgag ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt
    21001 gccgatgggc tactggaaag gttctggcat gtcgattgtg ctggatatga tcgctactct
    21061 cctttccgac ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat
    21121 ttcacaaatt tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc
    21181 caagctgcaa cgtatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc
    21241 catccgctta cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggcat
    21301 cactgttgat gacagcgtgt gggccaaaat ccaggcgtta tgaggagata agtcatgatt
    21361 tttggacata tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc
    21421 gattttctgc gcgctaccaa cttcaacgtc ctggagccgg gcgttgtcga aatcgacggc
    21481 aagaatattt atgcgc
//
LOCUS       DAJTNP010000064        21246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTNP010000064 DAJTNP010000000
VERSION     DAJTNP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            11..784
                     /locus_tag="OO160_002621"
                     /pseudo
     CDS             11..784
                     /locus_tag="OO160_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(844..2142)
                     /gene="eno"
                     /locus_tag="OO160_002622"
     CDS             complement(844..2142)
                     /gene="eno"
                     /locus_tag="OO160_002622"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5524687.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2230..3867)
                     /gene="pyrG"
                     /locus_tag="OO160_002623"
     CDS             complement(2230..3867)
                     /gene="pyrG"
                     /locus_tag="OO160_002623"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5524688.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4095..4886)
                     /gene="mazG"
                     /locus_tag="OO160_002624"
     CDS             complement(4095..4886)
                     /gene="mazG"
                     /locus_tag="OO160_002624"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5524689.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4982..7216)
                     /locus_tag="OO160_002625"
     CDS             complement(4982..7216)
                     /locus_tag="OO160_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5524690.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7264..8565)
                     /gene="rlmD"
                     /locus_tag="OO160_002626"
     CDS             complement(7264..8565)
                     /gene="rlmD"
                     /locus_tag="OO160_002626"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5524691.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8622..11378
                     /gene="barA"
                     /locus_tag="OO160_002627"
     CDS             8622..11378
                     /gene="barA"
                     /locus_tag="OO160_002627"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5524692.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11610..12950)
                     /gene="gudD"
                     /locus_tag="OO160_002628"
     CDS             complement(11610..12950)
                     /gene="gudD"
                     /locus_tag="OO160_002628"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5524693.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12971..14311)
                     /locus_tag="OO160_002629"
     CDS             complement(12971..14311)
                     /locus_tag="OO160_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5524694.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14313..15665)
                     /gene="gudP"
                     /locus_tag="OO160_002630"
     CDS             complement(14313..15665)
                     /gene="gudP"
                     /locus_tag="OO160_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5524695.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16099..16548)
                     /locus_tag="OO160_002631"
     CDS             complement(16099..16548)
                     /locus_tag="OO160_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5524696.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16566..17348)
                     /gene="truC"
                     /locus_tag="OO160_002632"
     CDS             complement(16566..17348)
                     /gene="truC"
                     /locus_tag="OO160_002632"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5524697.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17348..17677)
                     /locus_tag="OO160_002633"
     CDS             complement(17348..17677)
                     /locus_tag="OO160_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5524698.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18299..18844)
                     /gene="syd"
                     /locus_tag="OO160_002634"
     CDS             complement(18299..18844)
                     /gene="syd"
                     /locus_tag="OO160_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5524699.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18912..19760
                     /gene="queF"
                     /locus_tag="OO160_002635"
     CDS             18912..19760
                     /gene="queF"
                     /locus_tag="OO160_002635"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5524700.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19872..21236
                     /gene="ppnN"
                     /locus_tag="OO160_002636"
                     /gene_synonym="ygdH"
     CDS             19872..21236
                     /gene="ppnN"
                     /locus_tag="OO160_002636"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5524701.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5668 c         5065 g         5285 t
ORIGIN      
        1 ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga gtagcttaaa
       61 ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata atacccaagt
      121 gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac aatatgctac
      181 aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga tactgatcgt
      241 tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg aagaaaaggt
      301 aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat ttaaacaaca
      361 aaaattagct aagggattag agttagctat aatcgctttg aataaccaac tcacttcaca
      421 acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg atcattatta
      481 ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt ttcatcaagg
      541 cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc atcttttaga
      601 tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct tttttacttt
      661 caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat ctggtagaag
      721 ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat ccggccgctg
      781 gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca gataaagtca
      841 gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc
      901 ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc tcatagaacc
      961 agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag catcttcagt
     1021 ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca tcttgattgc
     1081 agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat
     1141 acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac
     1201 cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc agtcagattc
     1261 gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg
     1321 agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt atttaccatc
     1381 tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc ccagttcata
     1441 accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt
     1501 cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt tcagaacttt
     1561 tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc
     1621 aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct caccaccgtt
     1681 gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc
     1741 gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt tagccagaga
     1801 tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat
     1861 catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg cctgagcgat
     1921 cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc
     1981 gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc
     2041 cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga
     2101 gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca
     2161 ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt gccgttctaa
     2221 cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc
     2281 aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac
     2341 gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc
     2401 cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg
     2461 gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca ggctatcgtc
     2521 aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct
     2581 acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca caaccgggta
     2641 cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa catggcgagc
     2701 gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa tattgttctc
     2761 acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg
     2821 tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga
     2881 atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc
     2941 gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac cgatggtgac
     3001 ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca gatttgcttc
     3061 cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag atttcaacag
     3121 gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac
     3181 attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat ctgaacgaca
     3241 aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg
     3301 tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt gcataaacag
     3361 agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga acggcaagga
     3421 ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac cttcgccacc
     3481 ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca cggttgcgcc
     3541 gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa
     3601 gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca ggtccaagtc
     3661 ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc
     3721 tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc cacgggcttc
     3781 aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc
     3841 ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat
     3901 ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca aactttcatt
     3961 atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg
     4021 taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atggcgcttg
     4081 accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc
     4141 attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact
     4201 tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc tgctttcgtc
     4261 cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc ctcctccagt
     4321 ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat ctcttcgtag
     4381 actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc gcaacgtttc
     4441 tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc cagcgccgaa
     4501 tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc aagcacttca
     4561 ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc taatttatcg
     4621 ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc catttgcgcg
     4681 taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc aaaatcttca
     4741 cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca
     4801 aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg ctgcataata
     4861 gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt ctgtcaggtg
     4921 taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca gctatgtaag
     4981 actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg agtttaccca
     5041 gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt
     5101 gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg ttggcgagaa
     5161 tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg accaccagcg
     5221 aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct ggcgcatggg
     5281 agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg cgtccctggg
     5341 taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg atgtggtgca
     5401 tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg ttttgcggtg
     5461 tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct
     5521 tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc ccgccaccaa
     5581 tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga tgtttttctg
     5641 cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc cgcccagcca
     5701 gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa cgcccacggc
     5761 tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc ggctgtttct
     5821 gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc acaatgcgcc
     5881 cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg taagcgaagt
     5941 ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta aagacataca
     6001 cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa tcagccatct
     6061 cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg
     6121 cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc aactccgcat
     6181 cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc ggccccagaa
     6241 ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact
     6301 catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa caatcctgta
     6361 aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag gcgaggttct
     6421 ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac acttcagctt
     6481 taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg atatagtgtt
     6541 cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg gtcgggtgga
     6601 gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca agacggttcg
     6661 ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca tcttccggcg
     6721 catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact acgcagcgaa
     6781 aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg gaggaaacag
     6841 aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg ccgtgaataa
     6901 ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact acgttggcat
     6961 cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga
     7021 ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga tgcccctgcg
     7081 tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc tggctggtaa
     7141 tacccagact tgcgatccat ttttccggat caaattcacc agccttattg atatgtgcac
     7201 ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat cggcaaatcg
     7261 taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt gtgggaacat
     7321 atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt cgctatcccg
     7381 agccagcgtt gcagggttac aggatacata aactatacga ataggttcca gttttataat
     7441 ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt tatcgaagcc
     7501 gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac
     7561 attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg caggaacacc
     7621 ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt tgcccatacc
     7681 gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca acgcacgcgc
     7741 taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc taaaagttaa
     7801 gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga gtatctcact
     7861 atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg
     7921 atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc tggttgcctg
     7981 taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac atgccctgac
     8041 tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga cgtcgacaat
     8101 gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt
     8161 cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca cttcagagac
     8221 atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta aatccacgct
     8281 ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg gcgtttcgcg
     8341 ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt ttttatcttc
     8401 agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata acgttttgcc
     8461 gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa cggttatgat
     8521 ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg agactttctc
     8581 aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac tacagcctgc
     8641 gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta ttgctgagta
     8701 tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat gccggtgcta
     8761 gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa aatcgcgaat
     8821 ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt cgcgcgattt
     8881 cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt gatccctcat
     8941 caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact cgtgacggcg
     9001 atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac gaatcgccca
     9061 gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag ctggatctta
     9121 aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg atgctgtttt
     9181 gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc ggtccgattc
     9241 gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc cgggtggaag
     9301 gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg gcaatgtcgc
     9361 tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc gatctgcgtg
     9421 aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa aagcgcgccc
     9481 aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag ctgcgtacac
     9541 cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta acaccaacgc
     9601 agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca attattaatg
     9661 atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt attccattcc
     9721 cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct cacgataaag
     9781 ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc ggcgacccac
     9841 tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc accgagaatg
     9901 gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag
     9961 tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc ttattccagg
    10021 ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg ctggggctgg
    10081 tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat agccagccga
    10141 atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac attattatcg
    10201 aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa ccaaactccg
    10261 cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg gtttatagcc
    10321 caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc atcgcggtga
    10381 ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta tcgatgaccg
    10441 atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa ctcaagcaag
    10501 atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg cctgccctga
    10561 cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg
    10621 caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc ggcgcattgc
    10681 tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca gttgaacggg
    10741 cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac atggatggca
    10801 ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg gttatcgcgg
    10861 taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg atgagcgatt
    10921 atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc tacaagcctg
    10981 gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg gtgaacccga
    11041 atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc gatttagcgc
    11101 gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac
    11161 agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg catggcagtt
    11221 gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa caacagctac
    11281 gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg gacgagatgg
    11341 ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt cgagtagcaa
    11401 tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt atgccggatg
    11461 cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt aaaaaatgat
    11521 gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct caaaccggct
    11581 ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg cttgttatcg
    11641 aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc gccaagcccg
    11701 tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc tacacccagc
    11761 cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc tttggtcagg
    11821 cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt acccggtgca
    11881 gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt tgagtgtgaa
    11941 ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc ttgcatagtc
    12001 cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt atggcccatc
    12061 tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc gcgacggaac
    12121 tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc ttctgcataa
    12181 gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga ccaggcaccg
    12241 ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac aatagactcg
    12301 gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa gccatatttt
    12361 tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc ttcatgacgc
    12421 aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg tgtggctttg
    12481 cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg ttgaccatcg
    12541 cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag cattgccgct
    12601 tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa accgcgccca
    12661 ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt gtattcaccc
    12721 agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat tttctcgccg
    12781 ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac aatattacgc
    12841 gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg acccgccacc
    12901 gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat atttatgtcc
    12961 tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc cagccgggga
    13021 tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt ttataggcct
    13081 catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc gcatcaggaa
    13141 cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa tcgccctcct
    13201 gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg cccacatggg
    13261 taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag gtcagccccc
    13321 agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag tgcggatcgg
    13381 caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc cagttggtgg
    13441 cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc atcacttcac
    13501 gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga acatcattca
    13561 gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga tcaacggtaa
    13621 tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct tgctcgccag
    13681 gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt gaggcttccg
    13741 ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga taccactcat
    13801 ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca cctatataaa
    13861 acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac agttcgcaga
    13921 ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc gccgccacgg
    13981 cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa tcggctgcct
    14041 gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc tggcccagaa
    14101 ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc gcttcaccaa
    14161 tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag tatgcgttat
    14221 gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg actttcatat
    14281 cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg tttcagctcg
    14341 atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag ggcatgaaca
    14401 ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata accaattgcg
    14461 attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc ggaaagaccg
    14521 ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc gatgccctta
    14581 ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac gtagttgcag
    14641 aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg cgcaatgttc
    14701 agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac accgccgata
    14761 aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga catcccacgc
    14821 gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc gttgatacag
    14881 tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt gatctgcccc
    14941 cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt gatcagcgcg
    15001 ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg atttggctcg
    15061 tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat aaagaagaag
    15121 acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa gatcaccgtt
    15181 gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg caccggaaac
    15241 caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag cccgaccagg
    15301 aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc gacgaagcct
    15361 tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg ttttgaacca
    15421 aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca tgagaaggca
    15481 gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat ttccgaaccg
    15541 gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa caacatcacc
    15601 actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg acttaaagaa
    15661 ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact gcgacatcgc
    15721 caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt tcgccgaata
    15781 cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg gctcaccgtg
    15841 caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc ctggacggtg
    15901 gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa cgaagaaata
    15961 aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa tccgcgctat
    16021 gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct aaaacgacat
    16081 ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc acggatttga
    16141 ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc caacccgctg
    16201 agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa aatttacata
    16261 actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc ccagactatc
    16321 tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc cggtcgtgga
    16381 cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt caggatcttc
    16441 aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt ccgccactaa
    16501 cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac cgttttctcc
    16561 ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc aaccctttca
    16621 ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca ggtgtcgtcc
    16681 aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga caactgactg
    16741 gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt ctggcgtaaa
    16801 tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg tcgtcgaagc
    16861 tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt ggtcgggtaa
    16921 cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt cactgctggc
    16981 tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc ttccaccagt
    17041 ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg gtaacgtttc
    17101 tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct ggataatccc
    17161 atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac atgctgtcct
    17221 atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag ccagctacgg
    17281 tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg atagagtatt
    17341 tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac tcagccagaa
    17401 taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt gcaacagcaa
    17461 aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg atcagccccc
    17521 actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta ctattaaatt
    17581 gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac gcttcaagcg
    17641 cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa taagaaaaac
    17701 tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg ctcccggttc
    17761 gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa cttttcctct
    17821 ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca ccccgatgtg
    17881 atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct gaccggttct
    17941 cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc gcttcatcca
    18001 gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc atcctgatgt
    18061 tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg cgaagtctta
    18121 caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag aattaacaat
    18181 caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt cgcttatttc
    18241 ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg tatttggatt
    18301 acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa ttcgtccgtt
    18361 tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc gagatgacct
    18421 ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc cttttctgag
    18481 tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca ctccaggttt
    18541 gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca aactgagtcg
    18601 tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca accgcgttta
    18661 cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg gtagttgaaa
    18721 taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt ttatgctctt
    18781 catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg gtcaaatcgt
    18841 ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta tctggtttat
    18901 gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg actcttggaa
    18961 aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt ccacgcagcc
    19021 tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt acggatatct
    19081 ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc gctgtcggtc
    19141 atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt aagctctatc
    19201 tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag acgctggagc
    19261 gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt cttgatgaac
    19321 tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgaccag gatatcacta
    19381 tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt gaaaaagtag
    19441 tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc catcaaccag
    19501 attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa aaactgctgc
    19561 gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg gaacgcatct
    19621 ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca cgttataccc
    19681 gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc ccatcgacca
    19741 caagactggt tcggcaataa attttttctc aattttgcgt gctggattca cgcagaaggt
    19801 tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat tcgtcccata
    19861 aggagttttt cttgattaca catattagcc cgcttggctc catggatatg ttgtcgcagc
    19921 tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg tttcgcaact
    19981 gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa ttgctgtctc
    20041 gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag ctggaactga
    20101 ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg caggccaact
    20161 tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccataat accgttcgtt
    20221 ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt tccatcttgc
    20281 gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc tggggcggtc
    20341 actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag ctgggcctgc
    20401 gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg atgaaaggtg
    20461 ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt atgacagagc
    20521 cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc atcatgccgg
    20581 atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt atcttccctg
    20641 gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg aacccggcca
    20701 acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc gactacttcc
    20761 gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc cattaccgca
    20821 tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg ccgctggtga
    20881 aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg cgcattgcgc
    20941 cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg aagctttacc
    21001 cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc ggtattgtgg
    21061 cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct tacaaaatca
    21121 acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt gttgcccagc
    21181 atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc acgtaatctc
    21241 ccctcc
//
LOCUS       DAJTNP010000065        21098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTNP010000065 DAJTNP010000000
VERSION     DAJTNP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..852
                     /locus_tag="OO160_002637"
     CDS             <1..852
                     /locus_tag="OO160_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5524702.1"
                     /translation="VKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVK
                     KRIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRG
                     EVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
                     PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCL
                     DNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRN
                     QTYMPRV"
     gene            954..1106
                     /gene="hokA"
                     /locus_tag="OO160_002638"
     CDS             954..1106
                     /gene="hokA"
                     /locus_tag="OO160_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5524703.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1293..1505)
                     /gene="cspA"
                     /locus_tag="OO160_002639"
     CDS             complement(1293..1505)
                     /gene="cspA"
                     /locus_tag="OO160_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5524704.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1786..2076)
                     /locus_tag="OO160_002640"
                     /pseudo
     CDS             complement(1786..2076)
                     /locus_tag="OO160_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2510..3220
                     /locus_tag="OO160_002641"
     CDS             2510..3220
                     /locus_tag="OO160_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5524705.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3270..4244)
                     /gene="ghrB"
                     /locus_tag="OO160_002642"
     CDS             complement(3270..4244)
                     /gene="ghrB"
                     /locus_tag="OO160_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5524706.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4348..4998)
                     /locus_tag="OO160_002643"
     CDS             complement(4348..4998)
                     /locus_tag="OO160_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5524707.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5161..7494
                     /gene="bisC"
                     /locus_tag="OO160_002644"
     CDS             5161..7494
                     /gene="bisC"
                     /locus_tag="OO160_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5524708.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7463..7903)
                     /locus_tag="OO160_002645"
     CDS             complement(7463..7903)
                     /locus_tag="OO160_002645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5524709.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7900..8463)
                     /gene="tag"
                     /locus_tag="OO160_002646"
     CDS             complement(7900..8463)
                     /gene="tag"
                     /locus_tag="OO160_002646"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5524710.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8621..9319
                     /locus_tag="OO160_002647"
     CDS             8621..9319
                     /locus_tag="OO160_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5524711.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9548..10756
                     /locus_tag="OO160_002648"
     CDS             9548..10756
                     /locus_tag="OO160_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524712.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            11080..12771
                     /gene="eptB"
                     /locus_tag="OO160_002649"
     CDS             11080..12771
                     /gene="eptB"
                     /locus_tag="OO160_002649"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5524713.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12863..12939
                     /locus_tag="OO160_002650"
     tRNA            12863..12939
                     /locus_tag="OO160_002650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12897..12899,aa:Pro,seq:cgg)
     gene            13683..15290
                     /gene="dppA"
                     /locus_tag="OO160_002651"
     CDS             13683..15290
                     /gene="dppA"
                     /locus_tag="OO160_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5524714.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15598..16617
                     /gene="dppB"
                     /locus_tag="OO160_002652"
     CDS             15598..16617
                     /gene="dppB"
                     /locus_tag="OO160_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5524715.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16627..17529
                     /gene="dppC"
                     /locus_tag="OO160_002653"
     CDS             16627..17529
                     /gene="dppC"
                     /locus_tag="OO160_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5524716.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17540..18523
                     /gene="dppD"
                     /locus_tag="OO160_002654"
     CDS             17540..18523
                     /gene="dppD"
                     /locus_tag="OO160_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5524717.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18520..19524
                     /gene="dppF"
                     /locus_tag="OO160_002655"
     CDS             18520..19524
                     /gene="dppF"
                     /locus_tag="OO160_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5524718.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19554..20825)
                     /locus_tag="OO160_002656"
     CDS             complement(19554..20825)
                     /locus_tag="OO160_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5524719.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5114 a         5179 c         5512 g         5293 t
ORIGIN      
        1 gtgaaagtac tcaacgagct aaggcagttt tatcctcttg atgagcttct cagggctgcg
       61 gagataccgc gcagtacgtt ttattaccat ctaaaggctc tcagcaagcc tgacaagtat
      121 gcggacgtta aaaagcgtat tggtgagatt tatcacgaga atagaggccg atacggatac
      181 cgtagggtaa cgctgtctct tcatcgagaa gggaaacaga ttaaccataa agctgttcag
      241 cgcctgatgg gaaccctctc acttaaagca gcgattaagg tcaagcgata ccgctcttac
      301 agaggagagg tagggcaaac cgcccctaat gttctccaaa gagatttcaa ggctacgcgg
      361 ccaaacgaga agtgggttac cgatgttact gaatttgcag tcaatgggcg caagctgtat
      421 ttgtctccag taatagatct cttcaacaac gaagttattt cttacagcct ttcggaaaga
      481 ccagtgatga acatggttga gaatatgctc gatcaggcat tcaaaaagct taatcctcac
      541 gagcatcctg ttctgcactc tgaccaggga tggcagtatc gtatgagaag atatcaaaat
      601 atccttaaag aacatggtat taaacaaagc atgtccagaa aaggcaattg tctggataat
      661 gctgtggtgg agtgtttctt tggaacctta aagtcggagt gtttttatct tgatgagttc
      721 agtaatataa gcgaactgaa ggatgctgtt acggaatata ttgaatacta taacagcaga
      781 agaattagcc tgaaattaaa aggtctgact ccaattgaat atcggaatca gacctatatg
      841 cctcgtgtat aactgtccaa ctttttgggg tcagtacaaa gtgaggctac cctatgcctg
      901 aacaccataa ggttagcctc ttactcgttg gaaatcaaca cagggggctg ggaatgccgc
      961 agaaatatag attactttct ttaatagtga tttgtttcac gcttttattt ttcacctgga
     1021 tgataagaga ttcactgtgt gaattgcata ttaaacagga gagttatgag ctggcggcgt
     1081 ttttagccta caaattgaaa gagtaagagt cttcgacggg aaattattcc cgccttactt
     1141 acggcgttgc gcattctcat tgcacccata tttattcttc acaaaaataa taatagattt
     1201 tattacgcga tcgattattt atttcctgaa aaaaaataaa aaaatccccg ccaaatggca
     1261 gggatcttag attctgtgct tttaagcaga gattacaggc tggttacgtt accagctgcc
     1321 gggcctttag cgccgctttc gatggtgaag gacactttct gaccttcgtc cagagatttg
     1381 taaccatcgt tctggatagc agagaagtgt acgaacacat ctttagagcc atcgtcagga
     1441 gtgatgaagc cgaagccttt gtcagcgttg aaccatttta cgataccagt cattttaccg
     1501 gacatagtgt attaccttta ataattaagt gtgcctttcg gcgatatggc gtgctttaca
     1561 gattttgaag cgttaaagga atgtgcacta cgaggggtat caacgataac tcttgaaggg
     1621 acttgcctta ctacactgct ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc
     1681 actcattggc gggtgatgca acaattattt ttcatattta tgattaatcg gttggaaaac
     1741 ggtcctgtca tcaggaccgt aaacagcaat aaagtggata aaagtctatt tcatcaactg
     1801 cttacttaat gccgggttgg cttgaatcaa acgcatcaat tttagttcgg cacttgaagg
     1861 cttcacgcgt ctggattccc attcctttac catggcgact gatacgccca aaacccgggc
     1921 gaaatcatcg atttttaatc ctgtcccttt tcataactgc tcaatttcgg tacaggacgt
     1981 tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta aaaacaatct gttccaggct
     2041 gctcaacagc tcatgcattg gatctttata ttccattgaa aactcctcaa atcacactgc
     2101 gggatcgtga attgcatcga agctcattaa gaatagtcgg gaaaagcaga cacagcgggg
     2161 tgaagtcagg gcatgctctg gctatgattc gattcagcct gtcatttagc gtgacttata
     2221 gccacctaac gatctgataa atgccgatat atctcttatc actcctgttt tgtaaagatt
     2281 tcgcaaatct aaccttctgc gaaggcgtaa atctctggct acaaaaaagg caacggcact
     2341 atcatttttt aacaatgaaa caacaaatta accttgtttc attttctcca tgattttacc
     2401 aggaagatta cgtagaaagg gtatcctgca agcctgtcct ggactatcct tgtcacgtca
     2461 gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg
     2521 aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag
     2581 tgggtgtttt gataaagaag gcgatcagcg taaagcgttt atcgacttcc tgcaaaatac
     2641 agtgatgcgt agcggtgaac gtctaccaac cctgactgcc gatcagaaaa aacagtttgg
     2701 tccttttgtc tctgattacg cgattctgta tggttattct cagcaggtga atcaggcgat
     2761 ggattccggt ctgcgcccgg tcgtagacag cgttaacgca attcgcgtac cgcaggatta
     2821 cgttacgcaa agcggtccgc tgcgtgaaat gaatggttcg ctgggtgtgc tggcgcaaca
     2881 actgcaaaat gcgaagttgc aggccgatgc tgcgcactct gcgctaaaac agagcgatga
     2941 cctgaaaccg gtatttgatc aggcattcac caaagtggta acgacgccag ctgatgcatt
     3001 gcaaccgttg attccggcgg cacaaacttt cacgcaacaa ctggtgatgg ttggagacta
     3061 cattgctcag caaggtactc aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc
     3121 acagcaggca agtgaatata acaaactgat tgcgccatta ccggcacaac atcaggcgtt
     3181 taatcaggcc tggactacag cagttactgc aacccaataa agagtaaaag cccgagcgat
     3241 tatcgggctt tacttcacgc agtcgcagct tagtccgcga cgtgaggatt cacacagttc
     3301 ttctcaacct ttccttgtaa cgcatcaatc aaattatcca cggcacaggc ggccatgcca
     3361 taacgcgtct catgggtggc agatccaata tgcggtactg cgacgacgtt ggccattgag
     3421 agcaacggcg aatctacgga cagtggctct tgttcgaaga catccagccc ggcggcgtga
     3481 atttcgcctt tctgcaatgc tgcgatcagt gcattttcgt caaccaccgg gccacgtccg
     3541 gcattaatga aaatggcgga ggatttcatt ttggcgaatt gttctgcgcc aaacagatga
     3601 tgcgtctcat cagttaacgg caggatcagg caaacgaaat ctgactcttg taacagagta
     3661 tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg
     3721 ttatagagga tgggcatgtt gaagccaaag tgcacacgtt gtgccagcgc catgccgatc
     3781 cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta ccagtccggg
     3841 cctatgctcg cggtccattc gcctgctttt acccgttctg ctacctccac aacccgacga
     3901 gcggtagaca acaccagcgc catcagcgta tcggcgacgg tttctgttaa tacggttggc
     3961 gtgtgcatca gcagaatttt tcgggcggta agcgcatcga catcaaaatt gtcatagccg
     4021 acggagatcg ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc ggcatctaca
     4081 ttctcgtttg aacccagtaa accttcagct tctgcaaaaa ttgctgcatt ttgaacgacg
     4141 gtttgtgggc tgaggtttgc cacctggtga acggtgaaat gcgcttgcag gcgttgcagt
     4201 aaatcatcag gtaaggcttt gtagaggata acggacggct tcatgcttct ctccattcac
     4261 tgataaggcg atcaccttag cctgaaatat gatgaaggaa aactgaagaa tgccaggtga
     4321 tttctgcatc acctggcatg aaagggatta cagcgggctt aaggtaattt ctacacggcg
     4381 gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt gggttagccg ggccaaggcc
     4441 ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc
     4501 cgcacgctgc tgggagagac gcatgttcag gtcgtgaccc cccgtgctgt cggtataacc
     4561 aatcacgtta accgccgttt tcggatactc tttcagcacc attgccacgc cggtcagggt
     4621 gttagcgcct gccggtttca ggggagcgct gctgctgtcg aaggtcacat tgttcggcat
     4681 attgaggata atgttatccc cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc
     4741 acgcagcttc gcttcctgca catccatgta ataacccacg ccgccgccca gagctgcgcc
     4801 tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc ttcgaagaag agagcgcacc
     4861 aatacccgcg cccacgagag agcccagacc tgcgccgata gcagatttgc ctgcttcgcg
     4921 ttcgccggtg taagggttag ttgtgcagcc agataccgcc agagcgccac tcactacggc
     4981 ggcaagtaag ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgt
     5041 cacgggttcc gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact
     5101 cggaaatttg taagtaatat ttaactgctc aatacatcta acctttcagg agtcttcggt
     5161 ttggccaact catcctcacg atattccgtt ctgactgccg cccactgggg gcccatgctg
     5221 gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg cgttagccac aggaatggaa
     5281 aactccttgc agagcgcggt tcgcgaccag gttcacagca atacgcgggt acgatttcca
     5341 atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc aaggcattcg tgggcaggat
     5401 gaatttgttc gcgtgagttg ggatgaggcg ctggatctta ttcaccaaca acataaacgc
     5461 attcgtgagg cttatggtcc ggcatcgatt tttgctggtt cctacggctg gcgttcaaac
     5521 ggcgtgctgc ataaggcctc gacattatta caacgctata tggcgctggc aggcggttat
     5581 accgggcatc tgggggatta ttcgaccggt gcggcacagg cgatcatgcc gtatgtcgta
     5641 ggtggcagcg aagtttatca acagcaaacc agttggccgc tggtgctgga acatagcgat
     5701 gtcgtggtgt tgtggagtgc taacccactc aatacgctga aaattgcgtg gaatgcatcc
     5761 gatgagcagg ggctttctta cttttccgca ctgcgtgaca gtgggaaaaa gctgatctgc
     5821 attgatccaa tgcgatcgga aaccgtcgat ttctttggcg ataaaatgga gtgggtggca
     5881 ccgcacatgg gcaccgatgt tgcgctgatg ctggggatcg cccatacgct ggtggaaaat
     5941 ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag gttatgccgt cttcgcctct
     6001 tatttgctgg gcgagagtga cggaatagcg aaaaacgccg aatgggcggc agagatttgt
     6061 ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt tccaccaaaa taccaccatg
     6121 ctgatggcag gctggggaat gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc
     6181 gtcacgctgg cagcaatgtt ggggcaaatc ggcacacccg gcggcggttt tggtctttct
     6241 taccattttg ccaatggtgg taaccccacg cggcgttctg cggtgctctc ttccatgcag
     6301 ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa
     6361 gcactggaaa accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat
     6421 attcgtttta tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt
     6481 ctgatccgtg cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg
     6541 gcggcgaaac acgcggatat tgttctgcct gcgactacct cttttgagcg taatgatctc
     6601 accatgaccg gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca
     6661 cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag
     6721 ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat
     6781 aacgttgccc ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc
     6841 tggcaagcca accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc
     6901 ttcgctgatt tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt
     6961 gaaatcttct cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg
     7021 tggctggagc cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct
     7081 gcccatccgg cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac
     7141 gcggtagcaa accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcata
     7201 accgaagggg atatggtgcg ggtgtggaac tcccgtgggc agatccttgc cggagcggtc
     7261 attagcgagg gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg
     7321 gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc
     7381 cccagctcgc ggctggggaa tggctgtgcg ggtaatacga cgctggcatg gctggaaaaa
     7441 tacaacggtc cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg
     7501 tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt
     7561 aaaaatctat cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct
     7621 gctgcacata ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa
     7681 acattgctgc cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct
     7741 cttcccagac ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt
     7801 cacgccagta attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt
     7861 cgaggatcgc ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccagata
     7921 gcaacagcag ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca
     7981 gattgtggtg ccgacaaact taaaaccacg ttttttcagt gccttagata gggcgtcgga
     8041 ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg
     8101 atttacaaac gaccagacaa agtcgggaaa cggttcgccg ttctgttcca tttgcaggta
     8161 cgcccgcgca ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc
     8221 ctgtaccagt ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg
     8281 atgaaagtag gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc
     8341 ctgctgccct tcaaagcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc
     8401 ccactcatta tcatggtagg caatataaag cgggccctga ctcacccagc cgcaacgttc
     8461 catactttcc ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc
     8521 ggtgaaaaag tcaatattta atacagggct actatgcccg atatccttca tacaatgaca
     8581 ataatattgc cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg
     8641 tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac
     8701 agcttacgcc tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg
     8761 ttacacctgg gatagtgatc atcaacctga ttacaacgat attttgtcgc aacgtattca
     8821 aagtagccaa agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt
     8881 gaccagcagt atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc
     8941 ggtcgcggca ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag
     9001 tactaccacg ctgaccgatc cgttatggca tgccagcgtg agtagcttag gctggcgtgt
     9061 tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg
     9121 cgagaatatc tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg
     9181 caactggctg gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta
     9241 tgctgcgcta actcaggcag aaaataccac taataatagc gactatctgt atacgatggg
     9301 ggttagcgcc agattttaac gtaacagtca caattgaaac cattgaataa caacagttgt
     9361 ggcgatagtg ggtgctaact taccaaataa taaatttggt gaataattgt cgcgttattc
     9421 attcctgaac taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccaag
     9481 cataaaatgc acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa
     9541 tactgccatg acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat
     9601 cattacccag tttgcgctgg ggtcggttta tacccggagc ctgtttaatg gcgcgctttc
     9661 tgccaagctg gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg
     9721 gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt
     9781 caccgtggct tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa
     9841 cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta
     9901 cctgctgacg ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc
     9961 gttcgctatc ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct
    10021 gctcgaaacg gtgggtctgg aaaaaacctt tgtgatttgg ggagcgattg cgctggtgat
    10081 gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa
    10141 tggtgtggtg gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat
    10201 gttagcggta atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa
    10261 agatatcgcc caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt
    10321 tatttccatc gctaaccttt caggtcgtct ggtgctgggc attctgtctg acaaaattgc
    10381 ccgtatccgt gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct
    10441 gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt
    10501 tggcggcact attaccgtct ttccatcact ggtcagtgag ttcttcggcc tcaataacct
    10561 ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc ggtagcattt ttggttcgat
    10621 tatcgcctca ctgtttggcg gtttctatgt gaccttctac gtgattttcg ccctgctgat
    10681 tctgtcattg gcgctttcta cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc
    10741 gcatggctcc ctttaatgct cagccgcgag ctactgggct tcagcttccg tgtgcatcaa
    10801 acgcatcaac gaaatgtata aacaatgact cgtaaaattt gggccagtta actggccctt
    10861 tttactgctt tttgtaacgc taaagtctct tttcaaactt gcatttttgt aaatttgtgc
    10921 ttcatgcaca ctctttcccc acactttttc gctttgctgt ggtctactta ttcgcgcgtg
    10981 tagattttac ttatctgact acctccgcac ttttcccctg ccgggcctga aaagccgcta
    11041 agcagggtgt tatcacctgt ttgtccaggg tttgtttgca tgagatacat caaatcgatt
    11101 acacagcaga agctgagctt tttgcttgca atctatattg gcctttttat gaatggcgcg
    11161 gttttttatc gccgcttcgg cagctatgcg cacgatttta ccgtctggaa aggcgtttct
    11221 gctgttgttg aactggccgc caccgtactg gtgaccttct ttttactacg tcttctatcg
    11281 ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt
    11341 gccagctatt acatgacctt ccttaatgtg gtcattggtt atggcatcat cgcttccgtc
    11401 atgaccaccg atatcgacct gtcaaaagaa gttgttggtc tgaactttat tctctggtta
    11461 atcgccgtta gtgcattgcc tcttatcctt atctggaata accgctgtcg ctacaccttg
    11521 ctccgacaac tgcgaacccc agggcagcgt attcgcagcc tggtggtcgt cgtactggcg
    11581 ggtattatgg tttgggcacc gattcgtttg ctggatatcc agcagaagaa agtggagagg
    11641 gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag cgaactctta tctgccatca
    11701 aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat
    11761 aattcattgc ttaatccggc gaagaagttt acataccagg ctccacaaaa cgttgatgat
    11821 acttatgtcg tgtttatcat cggtgaaacc acgcgttggg accatatggg tattttcggc
    11881 tatgagcgta ataccacgcc gaaactggcc caggagaaaa atctggcggc gttccgtggt
    11941 tactcctgtg ataccgcaac caaactctca ctgcgttgca tgtttgtacg tcaggggggc
    12001 gcggaagata atccgcagcg cacattaaaa gaacagaaca ttttcgcggt tctgaagcag
    12061 ttaggattca gttctgacct ctacgctatg cagagcgaaa tgtggttcta cagcaacacg
    12121 atggcagaca acattgctta tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag
    12181 ccggtagatg atatgttgct ggtagacgaa atgcagcaat cgctagggcg caacccggat
    12241 ggtaagcatc tgatcattct gcataccaaa ggttcgcatt ttaactacac ccagcgttat
    12301 ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa
    12361 gcgcagatga tcaactccta tgacaactcg gtgacctatg tggatcactt tatctccagc
    12421 gtgattgatc aggttcgcga taagaaagcg attgtgttct acgcagctga ccacggtgag
    12481 tcaattaatg aacgcgagca cctgcacggc acgccgcgtg aactggcacc gccggagcag
    12541 ttccgcgtac cgatgatggt ctggatgtca gataaatatc tggaaaatcc ggccaatgcg
    12601 caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag tgccacgccg tcacgtagag
    12661 ctgtacgaca ccatcatggg ttgtcttggc tatacttcac cggatggtgg aattaacgaa
    12721 aacaacaact ggtgtcacat cccgcagaca aaagtggcag cggctaacta agcggcctgc
    12781 tgactttctc gccgatcaaa aggcattttg ctattaaggg attgacgagg gcgtatctgc
    12841 gcagtaagat gcgccccgca ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg
    12901 acgaaggggt cggaggttcg aatcctctat caccgaccaa attcgaaaag cctgctcaac
    12961 gagcaggttt ttttgcattt gtagctcatg aggatgagaa cctccggggg cagggaggct
    13021 cgactcgacg taggcctgat aagacgcgcc agcgtcgcat caggcaacgg caccgcactt
    13081 attgtcggat gcggcgcgac cgccttattc gaccaacaca ccacacattt tcctgccaac
    13141 tttgcgcccc atatctcact atccattaac ggatttgtga cagaaaccat tgtctttttt
    13201 tatatttgct taaatctttt ttgcgttact tatggctggc gctggcattt tccgctgtgc
    13261 gttttagcgt tcatggtatt aatcgctcag atatccacct tttcctcaga tttttttcat
    13321 taaatgcata aaacttaatc accaaatgtt gctaaatatt aagctgattg ttctggttat
    13381 gttggagtag cacaaatttc tctgctgcaa atagctgttt gaagtttttt taatctttgt
    13441 ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt attgacgttt tcacattctg
    13501 ttgacagatt gtaggtcacg aggggcattt tatggaggat ccgcactgtt acactgatgt
    13561 taattagtac ggcatcccca cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg
    13621 tcaggcagcg acaacccact gcaaagggtt aaaacaacaa acatcacaat tggagcagaa
    13681 taatgcgtat ttccttgaaa aagtcaggga tgctgaagct tggtctcagc ctggtggcta
    13741 tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta ttgctcagaa ggatctccgg
    13801 aagggtttaa cccgcagctg tttacctccg gcaccaccta tgacgcctct tccgtcccgc
    13861 tttataaccg cctggttgaa tttaaaatcg gcaccaccga agtgatcccg ggcctcgctg
    13921 aaaagtggga agtcagcgaa gacggtaaaa cctatacctt ccatctgcgt aaaggtgtga
    13981 agtggcacga caataaagaa ttcaaaccga cgcgcgaaat gaacgccgat gacgtggtgt
    14041 tctcgttcga tcgtcagaaa aacgcgcaaa acccgtacca taaagtttct ggcggcagct
    14101 acgaatactt cgaaggtatg ggcttgccgg agctgatcag cgaagtgaaa aaggtggacg
    14161 ataacaccgt tcagtttgtg ctgactcgac cggaagcgcc gttcctcgct gacctggcaa
    14221 tggacttcgc ctctattctg tcaaaagaat atgctgacgc gatgatgaaa gccggtacac
    14281 cggaaaaact ggacctcaac ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa
    14341 aagattcccg tatccgctac aaagcgtttg atggctactg gggcaccaaa ccgcagatcg
    14401 atacgctggt cttctctatt acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga
    14461 atgaatgcca ggtgatgccg tacccgaacc cggcagacat cgctcgcatg aagcaggata
    14521 aatccatcaa cctgatggaa atgccggggc tgaacgtcgg ctacctctcg tataacgtgc
    14581 agaaaaaacc gctggatgac gtgaaagttc gccaagcact aacctacgcg gtgaacaaag
    14641 acgcgatcat caaagcggtt tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc
    14701 caaccatgtg gggctataac gacgacgttc aggattacac ctacgatcct gaaaaagcga
    14761 aagccttgct gaaagaagcg ggtctggaaa aaggtttctc catcgacctg tgggcgatgc
    14821 cggtacaacg tccgtataac ccgaacgctc gccgcatggc ggagatgatt caggcagact
    14881 gggcgaaagt gggcgtgcag gccaaaatcg tcacctacga atggggtgag tacctcaagc
    14941 gtgcgaaaga tggcgaacat cagacggtaa tgatgggctg gactggcgat aacggggacc
    15001 cggataactt cttcgccacc ctgttcagct gcgccgcctc tgaacaaggt tctaactact
    15061 caaaatggtg ctacaaaccg tttgaagatc tgattcaacc ggcgcgtgct accgacgacc
    15121 ataacaaacg cgttgaactg tacaagcaag cgcaggtggt gatgcacgat caggctccgg
    15181 cactgatcat cgctcactcc accgtgtttg aaccggtacg caaagaagtc aaaggctatg
    15241 tggttgatcc attaggcaaa catcacttcg aaaacgtctc tatcgaataa ttaaaagcca
    15301 tacaagactg atggcaaagg caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa
    15361 attctgcaat gtattgaatt tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat
    15421 ccggcataaa caaagcgcac tttgtcaaca atctgtatac ccggtggcgc tgtgcctctg
    15481 cgtgcagcgc catccggcag caatacttca ttctctgtcc gacaagggcg ggaattgatt
    15541 tgtgagcaat acagacacgc agttccaggc tgcgagtcac tatagagaat ccgggttatg
    15601 ttgcagttta ttctccgacg tttgggactc gtcatcccca cgtttatcgg tattaccctt
    15661 ctcacatttg cctttgtcca catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa
    15721 cgtgggatct cccctgagcg tcacgtgcag ctgctggctg aactcggctt agataaaccg
    15781 atgtggcagc agtatctcca ttacatttgg ggcgtaatgc acggcgatct aggcatttca
    15841 atgaaaagcc gaattccagt atgggaagag ttcgtgccgc gctttcaggc cacgctggaa
    15901 cttggcgtct gcgcgatgat ttttgctacg gcagtcggta ttccggttgg tgtgctggct
    15961 gcggttaaac gcggttccat tttcgatcac actgcagttg gcctggcgct gaccggctac
    16021 tcgatgccga tcttctggtg gggcatgatg ctgatcatgc tggtttcggt gcactggaac
    16081 ctgacgcccg tctccggtcg cgtgagcgat atggtgttcc tcgatgactc caatccgtta
    16141 accggtttta tgctaatcga caccgccatc tggggtgaag acggcaactt tatcgatgcc
    16201 gtcgcccata tgatcttgcc cgccattgtg ctgggtacta ttccgctagc ggtcattgtg
    16261 cgtatgacac gctcctcgat gctggaagtg ctgggcgagg attacatccg caccgcgcgc
    16321 gccaaagggc taacccgcat gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg
    16381 ccggtagtga ccgttatcgg cctccaggtg ggaacattgc tggcgggggc gattctgacc
    16441 gaaaccatct tctcgtggcc cggtctgggg cgctggttga ttgacgcact gcaacgccgc
    16501 gactatccgg tagtgcaggg cggcgtattg ctggtggcga cgatgattat cctcgtcaac
    16561 ttgctggtcg atctgctgta cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg
    16621 gacatcatgt cacaggttac tgaaaataaa gtgattagcg caccggtgcc gatgaccccg
    16681 ttacaggagt tctggcacta ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac
    16741 gtcgccatcg tgctgttcat cgcgatcttt gccaactgga ttgcgcctta taacccggcg
    16801 gatcagttcc gcgatgcgct gctcgccccg ccagcctggc aggaaggcgg cagtatggca
    16861 cacttgctgg gcaccgatga cgtcggtcgt gatgtgctgt cgcggctgat gtacggcgcg
    16921 cgtttatcgc tgctggttgg ctgtctggtg gtggtgttgt cgctgattat gggcgttatt
    16981 ctcggcctga tcgccggtta cttcggcggc ctggttgata acattatcat gcgcgtggtc
    17041 gatatcatgc tggcgctgcc aagtctgctg ttggcactgg tgctggtggc gattttcggc
    17101 ccgtcgattg gtaacgccgc gctggcgctg accttcgttg ccttgccgca ctatgtgcgc
    17161 ttaacccgcg ccgccgtgct ggtggaggtg aatcgcgatt acgtcaccgc ctctcgcgtg
    17221 gcaggtgccg gggcgatgcg ccagatgttt attaacatct tcccgaactg ccttgcgccg
    17281 ctgattgttc aggcgtcgct cggtttctct aacgccattc tcgatatggc tgctcttggt
    17341 ttcctcggca tgggggcaca gccgccaacg cctgagtggg gcaccatgct ctccgacgtg
    17401 ttgcagttcg cgcaaagcgc ctggtgggtc gtgaccttcc ctggtctggc gatcctgttg
    17461 acggtgctgg catttaacct gatgggtgac ggtctgcgtg acgcgctcga tcccaaacta
    17521 aagcagtaag aggttcgaga tggcgttatt aaatgtagat aaattatcgg tgcatttcgg
    17581 cgacgaaagc gcaccgttcc gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga
    17641 agtggtcggg attgtgggtg agtccggctc cggtaagtcg gtcagttcac tggcgattat
    17701 ggggctgatt gattatccgg gccgcgtaat ggcagaaaaa ctggagttta acggccagga
    17761 tttgcagcgt atctcagaaa aagagcgccg caacctggtg ggtgccgaag tggcgatgat
    17821 cttccaggac ccgatgacca gccttaaccc gtgctacacc gtgggtttcc agattatgga
    17881 agcgattaaa gtgcatcagg gcggcaacaa aagtacccgc cgtcagcggg cgattgacct
    17941 gctgaatcag gtcggtattc ccgatccggc ttcgcgtctg gatgtttacc cgcatcagct
    18001 ttccggcggc atgagccagc gcgtgatgat cgccatggcg attgcctgtc ggccaaaact
    18061 gctgattgcc gatgaaccga ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga
    18121 actactgctg gagctacagc agaaagagaa catggcgctg gtgttaatta cccatgacct
    18181 ggcgctggtg gcggaagctg cgcataaaat cattgtgatg tatgcaggtc aggtagtaga
    18241 aacgggcgat gcacacgcca tcttccatgc gccgcgtcac ccgtatactc aggcattgct
    18301 gcgtgcgctg ccagaatttg ctcaggacaa agaacgtctg gcgtcgttgc caggtgttgt
    18361 tcccggcaag tatgaccgcc cgaacggctg cctgcttaac ccgcgctgcc cctatgccac
    18421 tgacagatgt cgcgctgaag aaccggcgct gaatatgctc gctgatgggc gtcagtccaa
    18481 atgccattac ccacttgatg atgccgggag gcccacacta tgagtacgca agaggccacc
    18541 tcgcaacaac cgctgttgca ggctatcgac ctgaaaaaac attatccggt gaagaaaggc
    18601 atgttcgtgc cggaacgtct ggtgaaggcg ctggacggcg tttcgtttaa ccttgaacgt
    18661 ggcaaaacgc tggcagtagt gggcgaatct ggctgcggta aatcgaccct cggtcggttg
    18721 ctgacgatga tcgaaacgcc caccggcggc gagctgtatt atcaggggca ggatctgctc
    18781 aagcacgatc cgcaggcgca gaagctgcgt cggcagaaaa tccagatcgt cttccagaat
    18841 ccctacggtt cgctgaatcc gcgtaagaaa gttgggcaaa ttctcgaaga gccgctgctt
    18901 attaacacca gcttaagcaa agaacagcgt cgggaaaaag ccctgtcgat gatggcaaaa
    18961 gtcggcctga aaaccgagca ctatgaccgc tatccgcaca tgttttccgg cggacaacgt
    19021 cagcgtatcg ctattgcccg tggtctgatg ctcgacccgg atgtggtgat tgccgatgag
    19081 ccggtttctg cgctggacgt ctcagtgcgt gcgcaggtgc tgaatctgat gatggatttg
    19141 cagcaggagt tggggctttc ttatgtcttt atctcccacg acctgtcggt tgtggagcac
    19201 attgctgatg aagtgatggt gatgtaccta ggccgctgcg tggagaaagg aacgaaggac
    19261 caaatcttca ataacccgcg ccatccgtac acccaggcgc tgctctccgc gacgccacgc
    19321 ctgaacccgg acgatcgtcg tgaacgcatc aaactcaccg gtgaactgcc aagcccgctg
    19381 aatcctccgc cgggttgcgc cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc
    19441 cagttgcagc cgcagctaaa agactacgga ggtcaactgg tagcttgttt tgctgttgat
    19501 caggatgaaa atccgcagcg ttaactcaca aaaccggggc catgcgctcc ggtttactca
    19561 atcagcttca acagtggcga caagcatagc aaaatgccgt aaagcagaat cagccaggct
    19621 ttaaatccgc gaagtttttc cagttgtgcg actttataga tcaggaaaaa cggaatgagg
    19681 cacgacacaa tgccatataa cgggcttccc aactgaaaga acaccagcac tgatacacga
    19741 aacgaaaccc aaatcgtcaa cgtaatgacg ataaaagcgc agatcgccag agtcagcatg
    19801 cgtgagttaa ttttcttggt atcaataatt cggcttaaca gattgagaat aatgccttta
    19861 atagcctcgt ggaaaccgag ataaatgcca aagaatgcgg tcagtacggc aaagatattc
    19921 agcaccgtag aggtgatatg aatgatatgt ccagggatca cctgcgcggc cagtgccagt
    19981 gccgagatat tttgttcaaa ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac
    20041 gaaaaggcaa aaaacaggat caccgcgatg agcgtgatat aactaattcg gtgggtacgc
    20101 agcgcgagcc gggttgccag tactttatct gcttcccgtt tacgataggc aatattcatt
    20161 gggttaagta cctgaataaa taccgcagaa aagaagcaaa atggaatggt aagcaggaca
    20221 tcgcggaaaa agacggaggc ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg
    20281 atcatcgcaa aaccgaacac gacaataatc cctactttga ccaccaccat tggcccagaa
    20341 atcttaaaca gtaatcgttc accaccagac gcaatcgcta tcagtacggc gaaaatagcg
    20401 actttataaa gtagagattg tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac
    20461 gaggcgctgt cgaaaaccac ggagagagag tagataaaaa tcccgtggat aatcatcaaa
    20521 aagtagataa ccccgaggaa aattccccag ttcttcccca ggtaatgact gataatatcg
    20581 gtgtagtcat tacaggaatc gctttcagaa agggttttta aataaatgtc ctgcactacc
    20641 caggtgacag gataagcaat gatcgctgcg gtaataaata cccaaattcc cttcaagcca
    20701 atttgtactg gcatcagcac ggttccggca ccaatcgcca tgcctatgca taataaaacc
    20761 cagccgaagt cgtagcgtgt aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc
    20821 tgcataaagt cctgtccaga aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc
    20881 ttgcgcaaca ggtggagaag tcgaatacgc agtatgtaaa aaatgaggca acagattcac
    20941 tggggaggtc gcgaggaggc gcaattctac aaaattgcgc atttcactac cagtctggag
    21001 tcagcccgga tatgaactgc aactattgca aataaagaaa ttaagctgga aggcatcgcg
    21061 caatgaggaa gattgccggg tgctgacagt aaaaagca
//
LOCUS       DAJTNP010000066        21075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTNP010000066 DAJTNP010000000
VERSION     DAJTNP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..448)
                     /locus_tag="OO160_002657"
     CDS             complement(<1..448)
                     /locus_tag="OO160_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5524720.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(503..1354)
                     /gene="manZ"
                     /locus_tag="OO160_002658"
     CDS             complement(503..1354)
                     /gene="manZ"
                     /locus_tag="OO160_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5524721.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1367..2167)
                     /gene="manY"
                     /locus_tag="OO160_002659"
     CDS             complement(1367..2167)
                     /gene="manY"
                     /locus_tag="OO160_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5524722.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2230..3201)
                     /gene="manX"
                     /locus_tag="OO160_002660"
     CDS             complement(2230..3201)
                     /gene="manX"
                     /locus_tag="OO160_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5524723.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3664..5214
                     /gene="yoaE"
                     /locus_tag="OO160_002661"
     CDS             3664..5214
                     /gene="yoaE"
                     /locus_tag="OO160_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5524724.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5218..6816)
                     /locus_tag="OO160_002662"
     CDS             complement(5218..6816)
                     /locus_tag="OO160_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5524725.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6947..8311)
                     /gene="sdaA"
                     /locus_tag="OO160_002663"
     CDS             complement(6947..8311)
                     /gene="sdaA"
                     /locus_tag="OO160_002663"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5524726.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8495..9073)
                     /locus_tag="OO160_002664"
     CDS             complement(8495..9073)
                     /locus_tag="OO160_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5524727.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9077..10438)
                     /gene="pabB"
                     /locus_tag="OO160_002665"
     CDS             complement(9077..10438)
                     /gene="pabB"
                     /locus_tag="OO160_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5524728.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10512..10691
                     /locus_tag="OO160_002666"
     CDS             10512..10691
                     /locus_tag="OO160_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5524729.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10811..11170)
                     /locus_tag="OO160_002667"
     CDS             complement(10811..11170)
                     /locus_tag="OO160_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5524730.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11532..11876)
                     /locus_tag="OO160_002668"
     CDS             complement(11532..11876)
                     /locus_tag="OO160_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5524731.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12008..13918
                     /locus_tag="OO160_002669"
     CDS             12008..13918
                     /locus_tag="OO160_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5524732.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13976..14671
                     /gene="tsaB"
                     /locus_tag="OO160_002670"
     CDS             13976..14671
                     /gene="tsaB"
                     /locus_tag="OO160_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5524733.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14711..15292
                     /gene="yeaY"
                     /locus_tag="OO160_002671"
     CDS             14711..15292
                     /gene="yeaY"
                     /locus_tag="OO160_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5524734.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15498..17183
                     /gene="fadD"
                     /locus_tag="OO160_002672"
     CDS             15498..17183
                     /gene="fadD"
                     /locus_tag="OO160_002672"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5524735.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17265..18380
                     /gene="rnd"
                     /locus_tag="OO160_002673"
     CDS             17265..18380
                     /gene="rnd"
                     /locus_tag="OO160_002673"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5524736.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18434..19399)
                     /gene="yeaX"
                     /locus_tag="OO160_002674"
     CDS             complement(18434..19399)
                     /gene="yeaX"
                     /locus_tag="OO160_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5524737.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19455..20072)
                     /gene="yeaW"
                     /locus_tag="OO160_002675"
                     /pseudo
     CDS             complement(19455..20072)
                     /gene="yeaW"
                     /locus_tag="OO160_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20414..20980)
                     /locus_tag="OO160_002676"
     CDS             complement(20414..20980)
                     /locus_tag="OO160_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5524738.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT         5199 a         5236 c         5417 g         5223 t
ORIGIN      
        1 atacgagaag cttataaatt ttttccagat cgtcgatatt tcgaacgcgg attaacagcc
       61 gacgctgttc taattgcatc accaacacgc catcttccga caagttcatc gctttgattc
      121 ggctatattc aatccagaca ttggcgaaga aaaaaccttt ttgtttaaag atgatcttcg
      181 gaatgcggat ccagaatata taaaaaccca tcagagccag tgcacttaat aaccatgttg
      241 ttattagcgc gccatgattc gtgacgttgt tatagataag aatgacaatc aatccgacga
      301 agataacgct atcgatgcga ccacgccgga gcaaaggaat tgccagcagg gtggggccgt
      361 tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc caggagtgcg gcgatgaaaa
      421 gaatcagtac caggtccgtg attgtcatta atcctccaga taaaaaaacg gggccaaaag
      481 gccccggtag tgtacgacag tcttacagtc ccagcaggcc gcaagcgtaa ccagcgatac
      541 cgatgacgaa gaagccaacg atgatccaca gcgggttaac ttttttgcgc agcagccaca
      601 tacaagcaaa tgtcagcagc agtggtacaa ggcctggcat taactggtca agaatagttt
      661 gcacggtggt gacacgggtc tgtccatcct gcccggtgat ggtcgaaacc accagcggga
      721 tgttgacatg tgtccacttg ttaaccaatg cccccatgac aaacaggccg aggatagacg
      781 ccccttccgt cagtttttgc aggaagccac cacccatatc tttaacgata tcgatacctt
      841 tggagtaacc atacgctacg ccgtagtaac gggttgccag acgcaccagg ttaaacagga
      901 tgaagaacag cagcggacct aacaggctgc cgctcatcgc gataccggca cccagtgctg
      961 caaataccgg acgtacggtt ccccagaaga tcgggtcgcc tacaccagcc agcggcccca
     1021 tcaaaccgac tttgataccg ttgatagcac cgtcatcgat ctctgcgcca ttagcacgct
     1081 gttcttccag cgccagggtt acgccgagaa tcggtgcagc cacgaacggc tgggtgttaa
     1141 agaactccag gtgacggcga atagcttgtt tacgagcttc gttgttctca gggtagaggc
     1201 gacgaattgc cggtaccata gagaagcaga aacccagtgc ctgcatacgt tcgaagttcc
     1261 atgaaccctg gaagaggtta gaacgcagga atacgccacg aatatcactt tgagtgagtt
     1321 ttttctcggt ggtagtttga gttgtatcaa ccatttcgct cacctgttag tccagttcgt
     1381 tatcgagatc gttgttacca gctgcctgag caggcgcacc ggctacgcgg ttgtatttcg
     1441 ggctaagttg gatgtagagc actgccataa cagtaccaat cacacccaga gcaaccaggt
     1501 taaagttggt gaatgctgcg gttacgaagc cgaggtagaa gaacggcatc aggtagccag
     1561 cacgcatcat gttgataacc atcgcataac caaccaccac gatcatgccc ccggcgatat
     1621 tcagaccatt ggtcaccact tccggaatcg cattcagcat gttctgtact tcgctggtac
     1681 caacagacag cgcaacgatg acggccggaa tagccacacg cattgcttgc aggaacagag
     1741 aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt atcagcagcc ttatccgcag
     1801 cgtgctggaa agcaacggta atagtacgaa cgatgatggt cagtacctgg cccgcagcgg
     1861 ccagagggat tgccagtgcg atacctgcac caatgctctg atgacctgcg ataaccagaa
     1921 tggtagaaat gatagaagcc agagcggcgt caggcgcaac tgcagcaccg atgttcatcc
     1981 agcccagcgc gatcatttcc agcgtaccac cgataataat accggttttc atatccccaa
     2041 gaacgatacc caccagggta cacgcgatta gcggacggtg aaactgaaat tcatcgagga
     2101 ttgatcccat acctgcgata caggctacga taaataccag cacaatttga agagtggtaa
     2161 tctccattgt acttctcctg tttacgtaag acttaagtgt gaaaactgaa tgataatcaa
     2221 cacaatacgt tacttatcga ttttgctgat cagatccatc attttcagtt tcggatcggt
     2281 ggaaacctta cggacttcca gctcaatacc gcgtgcattc agttttttga acgcctcgat
     2341 atctttttca tcaaccgaaa ccgcgttatt cacctgggtt ttaccctgac ggaatgccat
     2401 accaccgacg ttaacagagg tgattttcac gccgccttca acgagacgct ctacatctgt
     2461 tgggttggta aataacagca ttacgcgttc gccagcatat ttcgggttgt tgtagacgcg
     2521 aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc ggaggtgcaa cctgggtgag
     2581 cagtgtctta cgaacggtat ccgcagccac ttcatcacta acaacaataa tacgggagac
     2641 attggtttct ttggtccagc gggtggcgac ctgaccgtga atcagacggt cgtcgatacg
     2701 cgcaaggcca ataaccatgt agtcgtttgg ccccattggt tttgccggag ttggagccgc
     2761 ttttggtgct gcggcagcgg gtgctggcgc ggctttttca accggtttgg ctttcagtgc
     2821 tttcacgcct tcacggcctg tttctactgc cagcgcaacc agttcatcaa agctcgggtc
     2881 atcatcacgg gccattaacg tttccacgag cattggaatg ttaacgcctg caatgacttc
     2941 ataatgctct ttgtcgacga caatgcggct ggcagcattg aacgggctgc ctccccatgt
     3001 atcaacgaga aacagcacgc ctttagtggt gtcgagtttt gccaactgag cgttgtattt
     3061 ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg atccagccga cgttttcctg
     3121 ctcgcctaac agcatttctg ccgttttaag caactgctct gcagcccaac catgtgtgcc
     3181 tataacaata gcaatggtca cttgctacct cctttattat cgttaacacc tcaacgtgcc
     3241 agatgtattt ttgaatcgtc gtccacaatc gaatcgattc agataagggc tacaaccaaa
     3301 aacccctgcg tttcgcgatt tattttagat atcgaaaaaa ttattttatg tgatgaagat
     3361 ccgtaattta actttcgatt atcagaaatt tcgaaaggta aaatatcctt gtcacattcg
     3421 tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc tctgatatgt ttaactcccg
     3481 tttaacaacc atggagtata gggcagtagc ccgcagtatg gatcgtcacc gacgtcattt
     3541 cagcatcagg ccttttaacg cctgcctttc tggcactcta tgccgcacct ttcgtttgca
     3601 ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc tattccttaa gcaggagctt
     3661 gtcatggaat tcttaatgga cccctcaatt tgggcggggc tactcacgct tgttgttctc
     3721 gaaattgtgc tgggtatcga taacctggtc ttcatcgcca ttcttgctga caaactgccg
     3781 ccaaaacaac gcgataaagc gcgtttgctg gggttatcac tggcgctgat tatgcgtctg
     3841 gggctgctgt cgctgatttc atggatggtc acgctgacca aaccgctatt taccgtcatg
     3901 gatttctcct tctccggacg cgatctgatt atgttgttcg gggggatatt cttgctgttc
     3961 aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg atcatgattc cggccacggt
     4021 aaaggctacg ccagtttctg ggtggtcgtc acacagattg tcatccttga cgccgtcttc
     4081 tcgttggatg cggtaattac tgcagtaggg atggttaacc atctgccggt gatgatggcg
     4141 gcggtagtga ttgcgatggc ggttatgttg ctggcatcga aaccgctgac gcgattcgtt
     4201 aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc tgttaatgat tggtctgagt
     4261 ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt acctgtatgc cgcgattggc
     4321 ttctcgatca ttatcgaagt gtttaaccag attgcgcgtc gcaactttat tcgtcaccag
     4381 tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc tgcgtttgat gggcgggaaa
     4441 cgtcaggcca atgttcagca cgatgccgat aacccgatgc cgatcccgga aggtgcattt
     4501 gccgaagaag aacggtacat gattaacggc gtactgacgc tggcgtcgcg ttctctgcgc
     4561 gggatcatga cgccgcgcgg tgaaataagc tgggttgacg ctaatctcgg ggtcgatgaa
     4621 atccgcgagc aactgctctc ttcaccgcac agtctgttcc cggtatgtcg cggtgaactg
     4681 gatgaaatca tcggtatcgt acgtgctaaa gaactgctgg tggcgctgga agagggcgtt
     4741 gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg tcccggaaac cctcgatccg
     4801 atcaacctgc tgggcgtgct gcgtcgcgct cgcgggagct ttgttatcgt gaccaacgag
     4861 tttggtgtgg tacaaggctt ggtcacgccg ctggatgtgc tggaagccat tgcgggtgaa
     4921 ttcccggacg ctgacgaaac gccggaaatc attaccgatg gtaacggctg gctggtaaaa
     4981 ggcggtacag atttgcatgc cttgcagcag gcgcttgatg ttgagcacct tgccgatgac
     5041 gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa atggtcacat tccccgtgtg
     5101 ggcgatgtga ttgatgtagg gccactgcat atcaccatct ttgaagccaa tgattatcgt
     5161 gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg atgaagatga gtaagcgtta
     5221 acgtaacgac ataatgggcg tgatatgtcc attatgccgg gcgggcggcg gttggctgcc
     5281 cgccagccat ttgggaaaat cacgtagcgg catcggctgt gcgtacaaat acccttgcaa
     5341 aatatgcacg ccatgacggc gcaaatactt cgcctgttct tgcgtttcca cgccctccgc
     5401 caccagttca atattcagtc tttgccccag cgcgatgatg atatcggtca ccgtcgagtt
     5461 aaccgcgtca gaacctatag ctgcggtaaa tgacttatca attttcagca cgtcaggacg
     5521 taatgtttca agccaggaaa acgagctgtt gccggtgccg aagtcatcaa tcgccagttt
     5581 gacgttttta cgatgcagct cgcgggcaat ccgataatca acatccagta aggcatcgcg
     5641 ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg ctaaaccagt actgattgag
     5701 atcttttatc agcacaccac ggcgaaaatg gctgggtgcg acgttaatgc caacatgaaa
     5761 ctgactactc atcgggaaaa catggtgctg acgaatggtt tctgccatca catagtgggt
     5821 cagtggcaca attaaatgat gttcttccgc gataggaata aacacatccg gtgaaatcca
     5881 gccctgacgc ggattattcc agcgcagcaa aatctctaca ccaatacatt gctggctgcg
     5941 cgcattaagc aaaggctgac agaacaattc gaactcatgt tgcgccaggc ccagattaat
     6001 ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag gcgatatagc ccaccagtaa
     6061 acttagcagc accgccagcg gtaattgtgt tggaagatag tgccatgcca gcgccgtagc
     6121 ccccggacca tttacgttaa tggtaaaagg aaagctttgc gaagaaacct gatagttttc
     6181 attgtcttca ggttgcggaa gggaatctac cagcccatta ccatagagca aatgccgttt
     6241 gtccaccgtc aggctggcgc tactgatttg cggcagttgt ggctcaagga gcattgccgt
     6301 cagtaagtcg atgttaatca tctctatgac cccagcattg ctactgcccg ctgcaggcgt
     6361 ccattgaatc aaaaccggac tgcctttaat cagggcacgg tcgatcgtca ggcgtaaaag
     6421 tggttgcggt gcaggaagtt cagccagaat gtccacgacg ggcacattgc gataaccaaa
     6481 aatgctggag caataaagtg tgccgtcttg caccaggcca atggatcgca cagtttggag
     6541 ttttgccgcc tgtttacgta atggcaaatg ggcgacagag cagggcagac caatcagcgg
     6601 aagcaagact ttgctacctg cctgtagggg aagcagtact ttatctaatt cctctacagc
     6661 gtgattggcg aattgtacta cccgttgttg atttaagtta cgctgtgaaa taaatcgcac
     6721 gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta caaacaatca ttcgattacg
     6781 gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg gccaccctgt aagcgtatgg
     6841 cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa tattacagca gacgagaaag
     6901 cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa gaagtattag tcacactgga
     6961 ctttgattgc cagaccaccg cgtgaggttt cgcggtattt ggcgttcatg tccttaccgg
     7021 tttcgtacat cgtttcgatg accttatcca gcgagacgcg cggtgcactg gtgcggcgca
     7081 gagccatccg tgcggcgtta atcgccttca cagaggcaat ggcattacgc tcaatgcacg
     7141 gcacctgaac ctgccctgca accgggtcgc aggttaaacc aaggttgtgt tccatgccaa
     7201 tttccgccgc cacgcaaacc tgttccgggc taccgcccag cagttctgca agacccgcag
     7261 cagccattga acaggcaaca cccacttcgc cctggcaacc aacttccgca ccggaaatag
     7321 aggcgttcat tttatacaat gcaccaatcg cgcccgctgc cataaagtaa cgggtataga
     7381 tgtctgggct gaccgattca ataaagtggt catagtaagc cagcactgcc ggaacgatac
     7441 cgcaggcacc gttggttggc gcagttacca cacgaccacc ggcggcgttt tcttcgttaa
     7501 ctgccagcgc aaacatgttt acccagtcaa tgacattcat cggatcgtta gacagtttat
     7561 cgctggaaac taacatccgg cgcagggcag acgcacgacg tggcacgcgc agcgggcctg
     7621 gcagtacacc ttcggtgttc atcccgcgat cgatacatgc ctgcatggtt tgccagacat
     7681 gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc cagttcgttc tgcatagcga
     7741 gaccagacag cgaatagccg gtttcattac agtacgcgag cagttcggtg gcagatttga
     7801 acggatacgg cacgctcact tcgttggcag catcctgacc aaagtgttct tcatcgacga
     7861 taaaaccgcc gccgatggaa taataagttt tgctgtagac gacttcatcg ccgttatagg
     7921 cgtggatttg cataccgttt tcatgcagcg gcaggttgcc gttatgaaaa cgcatcccgt
     7981 tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag cagcagacgt tcgcgctctt
     8041 ttacgtcgcg aataaaaccg ggaatactgt cgatatccac ggtggcaggt tcgttacctg
     8101 caagacccat aataatggcg atatcggtgt ggtggccttt acccgtcagc gacagtgaac
     8161 cataaacgtc cacggcaacg cgagtaacgc tatccagtaa gcctttttcg accagatcat
     8221 cgacgaactg tttacccgcc ttcataggcc ctacggtatg ggaagatgag ggaccaatcc
     8281 ccaccttaaa catgtcgaat agactaatca cgataatact cctgacaagg tgactggact
     8341 tccagtaacg aactaataac agcgcatagt gtaagaggga acgcggcgac tggcttaact
     8401 attcacatga attaaactaa tcaaaaaccg cgatttaact aatattgcga tgattgtctc
     8461 aagtagtgtt ttgtagatgt aaagataagt atagtcaggg ggtcacacca atttgcagcg
     8521 ccagctcacg aattatgcct gcggtcattc cccatacaaa atactgttcg taccaggaca
     8581 gccatacccg atgtgaatcg ccacggcggt agatatctaa agggtgataa cgacccagat
     8641 gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac ttcatcttca ctggcgcgat
     8701 acggcagatc gggcgggata atgccgacca ctggggttac ttgatagcca gttacgctat
     8761 cgacgggcgg cagcacgccg ataacttcaa cggcggaagg cggtatagcg acctcttctt
     8821 cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt gtcatcgact gcacctccag
     8881 ggaatgccac ttgtccagcg tgtttacgca gatgaattga acgctgagtc agcaacaacc
     8941 ccggttgcgg tcgacggacg atggggatta acacggcagc ctgacgatga tttaggggtt
     9001 cccggttaat ttgcgggcgc aaaagttgaa agcgagataa aaaatcatca agcgtcaggc
     9061 tacggtattc cacgtcttac ttctccagtt ggcgtaatat cttattaact ttatcaaaag
     9121 tttcctgata ttccgcttct tcctggctat cggcgacaat tccaccgccc gcagagcagt
     9181 atatttgtcc gttaatggca gtcagcgtgc ggatagtaat gctggtatcc atgttgccgc
     9241 aaaagctcaa atagccaatg ctgccgcacc aggcattacg tcgctgcggt tccagttcgt
     9301 cgataatttc catagcccgt actttcggag ccccggttat tgagccacca ggaaaagctg
     9361 cgcgcagcag atcgctggcg tgtaactgtt ctggtagtcg cgccgttata gtgctgacca
     9421 gatgatgcac ggcagggaag ggttccacca cgaagagctc tggtactttt accgaaccgg
     9481 ctacggcaac acgaccgata tcattacgca ttaaatcgac aatcatcaga ttttcggcac
     9541 gatctttcgc tgagttcgcc agtttttctg cttgtttgct atcttcctga ggatcgggca
     9601 ggcgtggtag cgtgccttta atcgggcgag tctggatttc actattatca caaagaataa
     9661 accgctctgg cgaaaggctt aaaattgcac cctgttcaag acgtaaaaaa gcgctaaatg
     9721 gcgcgcggtt ggcctgatta agctgaagga atgcctgcca ttcatcgcca gaataggtcg
     9781 catgaaaacg ctgggcgaga ttcacctgat agcaatcacc gctgtgcaga tattcctgta
     9841 cctggcgaaa tttttcgccg tactgctcgt gggtcatatt ggattgccag tcggaagtga
     9901 gcgtgaaatc ttcctgcggc gagaattgct ggctttccag ccaggcccga cgggcattga
     9961 catcattatg actcagcaaa gaaactgtat gacgctggtg gtcgacaatg agcgcccaat
    10021 cgtagatacc cactgccata tccggcagaa cgatatcttg ttccgcaatt tctggcagtg
    10081 actcaaaacg gcggcccaga tcgtagccaa acaaccccag tgcgccgccc tgaaatggca
    10141 aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag cacctgctgg agcacctgta
    10201 gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact aacaacggtt tctttaccta
    10261 aagtggttaa agtgcaaatc ggatcggcga ccacaatatc aaagcggcta tacggatgat
    10321 cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg gcttaagcgg gagaaataaa
    10381 attcagcggc gtcctgacgc cagggtaaag taatcacagt gggagataac gtcttcatca
    10441 gtcctgactc tactggctat gtgccgggta gcatgaaata attagcgcca acaatttagc
    10501 aggagttaac aatgtttgca ggtttacctt cactcatcca tgaacagcag caaaaagctg
    10561 tcgagcggat ccaggaactg atggcacaag ggatgagtag cggccaggca atcgcgctgg
    10621 tggcagaaga gctgcgtgcc aaccactccg gcgagctaat cgtggcgcgt tttgaggatg
    10681 aagacgagta acacactaat ccgtatcgat ttcgttatag aagccgatga tcatttgatt
    10741 gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc ctgacccatt tatgctgata
    10801 taaatttaac tcagaccagt gcttttagtt cttcctgcat atatgttgaa aagtattcag
    10861 gatttttaat cacagagcgt tcacctgtct ccattttttt tgcccatcca accatttttt
    10921 ccgtgaaccc agggttccag ccttcctggt cagctcgcgc cgtaatatca gcggcactcg
    10981 cgggtactcc cagttctttt aaatatttaa agattccctt cgccgtgctt tcattcatcg
    11041 aacttggtgt tggcgctgat acatccatgg caccaatgaa atccagggct ttatctatta
    11101 cagcaggcat attcttatcc ttaaaattaa ccatgttatc gataaggcta tatatgacct
    11161 taatttgcat ctaatatcaa agggattata acgatatggc acgagttaac ctgttgacat
    11221 tcaggtgttt taaaggtagc agctcatggt gtgttatcca gtaagatgga gaaaattaac
    11281 ggtcagcccg cacgctataa ccagattgtt cagctaagaa agaaaaccag agttgctaat
    11341 caaagggcga acacaacggc atggttatgt ttatttatat agttagtttt atagataaaa
    11401 ttgaaattat aaatataaat aatgaattag cagaagcatc aacagtaata tcttattctc
    11461 tggtcgattt atgtaaaacc gaccagtatt atcgataaat tatcgtctga cttattttgc
    11521 ttcgataaag cttacaccgc agccacaatc ttaatttcaa ctttatactt cgggttcatc
    11581 aaacccgctt gtaccgtgca gcgcaccggc gcatgacccg cgacaaccca ggcatcccac
    11641 gctttattca tcgccgcgaa gtcgttttta tcggccagga aaatggtggc atccagaatg
    11701 ctcgatttat tgctgccctg tttttccagc acggcgtcaa tctgtgccag cgtgttggcg
    11761 gtttgctcaa aggcatcggc gtcgaggttt tccggtacac cagtgtagta gagcgtgttg
    11821 ttgtggatta ctacatcaga ccagcgggct tcagcatcga tacgaacgat agtcatcatt
    11881 ttgtcctcat tatacttcca ttttcgtgct gcaaagactg ccacaatgtg aggttgccgt
    11941 caccacttca actggcgaaa gcgccctcat cctgacataa tccctgttca aatcaacagg
    12001 gggtagtgtg acggacgatt ttgcaccaga cggtcagctg gcgaaagcga taccaggctt
    12061 taagccgcga gaaccacagc gacagatggc ggtagccgtc acccaggcga tagaaaaagg
    12121 ccagccgctg gtggtggaag caggaaccgg tacgggcaaa acctacgctt acctggctcc
    12181 tgcgctgcgg gcgaaaaaga aagtcattat ctcgaccggc tcaaaagcgt tgcaggatca
    12241 gctctacagc cgcgatttgc caacagtctc aaaggcattg aaatatacgg gcaacgtggc
    12301 gctgctgaaa gggcgctcaa actacctctg cctcgaacgt ctcgaacagc aggcgctggc
    12361 ggggggcgat ctgccggtac aaatcttaag cgatgtgatc ctgctgcgct cctggtctaa
    12421 tcaaatagtc gatggtgata tcagcacctg cgtcagcgtg gcggaagatt cacaggcgtg
    12481 gccgctggtc accagcacca acgacaactg tcttggcagc gactgcccga tgtataaaga
    12541 ttgctttgtg gtcaaagcac gtaaaaaagc gatggacgcc gatgtggtgg tggtaaacca
    12601 tcatctcttt ctggcggata tggtggttaa agagagtgga tttggcgagt tgatccctga
    12661 agcggacgtc atgatcttcg acgaagccca ccagctaccg gacattgcca gccagtattt
    12721 tggtcagtca ctctccagtc gacaactgct cgacctggca aaagacatca ccatcgccta
    12781 ccgcaccgaa ttaaaagaca cccagcagtt acaaaagtgc gctgatcgtc ttgcccagag
    12841 tgcgcaggat tttcgtctgc aactcggtga gccaggttat cgcggtaacc tgcgtgagct
    12901 gttagctaat ccgcaaattc agcgggcatt tttactgctc gatgacacct tggaactttg
    12961 ttatgatgtg gcgaaactgt cactggggcg ttccgccttg ctggatgcgg catttgagcg
    13021 cgccacgttg tatcgcacac ggctgaagcg gctaaaagag atcaatcagc cgggctacag
    13081 ctactggtac gaatgcactt cgcgccattt tactctggct ctcacgccgc tcagcgtggc
    13141 ggataaattc aaagagttaa tggcgcaaaa gcccggtagc tggatcttca cctcagcaac
    13201 gctgtcggtg aacgacgatc tgcatcattt cacctcgcgg cttgggatag aacaggcgga
    13261 gtcgttgcta ttgccaagcc cgtttgatta cagccgccag gcgttactct gtgtgccgcg
    13321 caatctgccg caaaccaacc agccaggttc tgctcgccag ttagcggcaa tgctgcgacc
    13381 gatcatcgaa gctaacaacg gtcgttgttt tatgctttgt acctcgcacg ccatgatgcg
    13441 cgatctggcc gagcagttcc gcgctaccat gacgcttccc gttttgttgc agggggaaac
    13501 cagtaaaggc caactgttgc agcagtttgt cagtgccggt aatgcgcttc ttgtggcaac
    13561 cagtagtttc tgggaagggg tggacgtgcg tggcgataca ttgtcattgg taattatcga
    13621 caaattgccg tttacctcgc cggatgatcc actgttaaaa gcgcgcatgg aagattgtcg
    13681 tttgcgcggt ggcgacccgt tcgatgaagt gcaactacca gatgccgtca ttactctcaa
    13741 acagggggta ggtcgactga ttcgcgacgc cgacgatcgt ggcgtgctgg tgatttgtga
    13801 ctatcggctg gtgatgcgtc cttacggcgc gacgtttctc gccagtctgc cgcccgcgcc
    13861 acgcacccgt gacattgccc gtgcggttcg tttccttgcg ataccatcct ccaggtaatt
    13921 tgttacggag tatggtatga tgcgcgcctt tttattgatc taagcacgaa gacccatgcg
    13981 aattctggct atcgataccg cgacagaggc ctgctctgtc gccctgtgga acgacggtac
    14041 tgtcaacgct cattttgagc tttgccctcg tgaacatact caacgaatct taccgatggt
    14101 gcaggatatc ctgaccacca gcggaacttc cctgactgat attaacgctc tggcttacgg
    14161 gcgcggcccc ggtagcttta ccggcgtgcg cattggtatt ggcatcgcgc aagggctggc
    14221 gcttggcgcg gaattaccga tgattggcgt ctccacgcta atgacgatgg cgcaaggggc
    14281 gtggcgcaaa aacggcgcaa cacgcgtgct gtcagccatt gatgcgcgaa tgggcgaagt
    14341 ttactgggcc gaatatcagc gtgatgaaaa cggtatctgg cacggtgaag aaaccgaagc
    14401 cgtactcaaa cccgaactcg ttcatgaacg aatgcaacag ctttccggcg aatgggtgac
    14461 ggtagggacg ggctggcaag cctggccgga tctcggtaaa gagagcgggc tggttttgcg
    14521 cgatggcgaa gtgttactcc ccgctgcaga agatatgctg ccgattgctt gtcagatgtt
    14581 tgccgagggt aaaacggtgg cggtggaaca tgccgagccg gtttatttgc gtaacaacgt
    14641 cgcatggaag aaacttccgg gcaaagaatg aatcttaaga gtatgccctg agaaaaaaag
    14701 gagtcgcatc atggcggttc aaaagaatgt tatcaaaggc atactggcag gtacgtttgc
    14761 gctaatgctg agcggttgtg tcactgtgcc ggacgccatt aaaggcagca gtaccacgcc
    14821 gcaacaagat ttagttcggg tgatgagtgc gccgcagctg tacgttggtc aggaggcacg
    14881 ctttggtggc aaagtggttg cggtacaaaa ccagcaaggg aaaacccgcc tggaaattgc
    14941 taccgtaccg ctggacagcg gagctaggcc gacgctggga gaaccttctc gcggtcgcat
    15001 ttatgccgat gtgaacggtt ttctggaccc ggtggatttc cgtggacaac tggttacggt
    15061 agtcgggcca atcaccggtg cggttgacgg caaaatcggc aatacgccct ataaatttat
    15121 ggtgatgcaa gtaacgggtt acaaacgttg gcatttaacc cagcaggtga ttatgccgcc
    15181 tcagccgatt gatccatggt tttatggcgg tcgtggctgg ccctatggct acggcggatg
    15241 gggctggtat aatcccggcc ccgcgagagt acaaacagtt gtaaccgaat aattgcttgt
    15301 ttttaaagaa tgcagaaaca gcggctggtc cgctgtttct gcattcttac ggtaaagata
    15361 aaaataaata gtgacgcgct tcgcaacctt ttcgttgggt aattatcaag ctggtatgat
    15421 gagttaatat tatgttaacg gcgtgtatat catttggggt tgcgatgacg acgaacacgc
    15481 attttagagg tgaagaattg aagaaggttt ggcttaaccg ttatcccgcg gacgttccga
    15541 cggagatcaa ccctgaccgt tatcaatctc tggtagatat gtttgagcag tcggtcgcgc
    15601 gctacgccga tcagcctgcg tttgtgaata tgggggaggt gatgaccttc cgcaagctgg
    15661 aagaacgcag tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg aagaaaggcg
    15721 atcgcgttgc gttgatgatg cctaacttat tgcaatatcc ggtggcgcta tttggcattt
    15781 tgcgtgccgg gataatcgtc gtaaacgtta acccgttgta taccccgcgt gagcttgagc
    15841 atcagcttaa cgatagcggc gcatcggcga ttgttatcgt gtctaacttt gcccacactc
    15901 tggaaaaagt ggttgataaa accgccgttc agcacgtaat tctgacccgt atgggcgatc
    15961 agctatctac ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgcttgg
    16021 tgccgaaata ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc
    16081 ggatgcagta cgtcaaaccc gaactggtgc cggaagattt agccttcctg caatacaccg
    16141 gcggcaccac aggtgtggcg aaaggcgcga tgctgactca ccgcaatatg ctggcgaacc
    16201 tggaacaggt caacgccacc tatgggccgt tgctgcatcc aggtaaagaa ctggtagtaa
    16261 cggcactgcc gctgtatcac atttttgccc tgaccattaa ctgcctgctg tttatcgaac
    16321 tgggtgggca gaacctgctt atcactaacc cgcgtgatat tccagggttg gtaaaagagt
    16381 tagcgaaata tccgtttacc gctatcactg gcgttaacac cttgttcaat gcgttgctga
    16441 acaataaaga gttccagcag ctggatttct ccagtctgca tctttccgca ggcggtggga
    16501 tgcctgttca gcaagtggtg gcagaacgct gggtgaaact gaccggacag tatctgctgg
    16561 aaggttatgg cctgaccgag tgcgcgccgc tggtgagcgt taacccgtat gatattgatt
    16621 atcatagcgg tagcatcggt ttgccggtgc catcgacgga agccaaactg gtggatgacg
    16681 acgacaacga agtaccaccg ggtcaaccgg gtgagctttg tgtcaaagga ccgcaggtga
    16741 tgctgggtta ctggcagcgt ccggatgcta cagatgagat catcaaaaat ggctggttac
    16801 acaccggcga catcgcggtg atggatgaag aaggattcct gcgcattgtc gatcgtaaaa
    16861 aagacatgat tttggtttcc ggttttaacg tctatcccaa cgagattgaa gatgtcgtca
    16921 tgcagcatcc tggcgtacag gaagtcgcgg ctgttggcgt accttccggc tccagtggtg
    16981 aagcggtgaa aatcttcgta gtgaaaaaag atccatcgct taccgaagag tcactggtga
    17041 ctttttgccg ccgtcagctc acgggataca aagtaccgaa gctggtggag tttcgtgatg
    17101 agttaccgaa atctaacgtc ggaaaaattt tgcgacgaga attacgtgac gaagcgcgcg
    17161 gcaaagtgga caataaagcc tgagcgttaa gtcagtcgtc agacgccggt taatccggcg
    17221 ttttttttga cgcccactaa agagaaaaca atttgaatta ccaaatgatt accacggacg
    17281 atgcgctggc ttctttgtgt gaagccgtcc gtgtctttcc ggcgatagcc ctggatactg
    17341 aatttgttcg tacgcgcact tattacccgc agctggggtt gattcaactt ttcgatggcg
    17401 agcatctggc gctaattgat cctctcggga tcaccgactg gtcaccgctg aaagcgatcc
    17461 tgcgcgatcc gtccatcaca aaatttctcc atgcaggcag tgaagatctg gaagtgttcc
    17521 tcaatgtctt tggcgaatta ccacaaccct tgattgacac gcaaatcctt gctgccttct
    17581 gcggacgccc gatgtcatgg ggtttcgctt ccatggtgga agagtattcc ggcgttacgc
    17641 tggacaagag tgaatcgcgc accgactggc tggccagacc gctgaccgaa cgtcagtgtg
    17701 aatacgcagc ggcggatgtc tggtatctgt taccgatcac cgccaagctt atggtagaaa
    17761 cggaggcctc cggctggcta cctgcggcgc tggatgaatg ccgcctgatg caaatgcgtc
    17821 gtcaggaagt cgttgcgccg gaagatgcct ggcgtgatat caccaatgcc tggcagttac
    17881 gcacacgcca actggcctgt ctgcaactgt tagccgactg gcgactgcgc aaggcgcgag
    17941 agcgtgatct ggcggtgaac tttgtcgtgc gtgaagagca tttgtggtcg gtcgcgcgtt
    18001 atatgccggg aagtttaggc gaactggaca gcctgggttt atccggtagc gaaatccgct
    18061 ttcacggtaa aacgctgctg gcgctggtgg aaaaagcgca gacattgccg gaagatgcct
    18121 taccgcagcc gatgcttaac ctgatggaca tgcctggtta tcgtaaagcg tttaaagcga
    18181 ttaagtcgct gattactgac gtgagcgaaa cgcataagat cagcgccgaa ttgctggcat
    18241 cgcgtcggca aatcaaccaa ctgctgaact ggcactggaa actgaaaccg cagaacaatt
    18301 tgccggagct gatttccggc tggcgtggtg agctgatggc ggaagcatta cacaatttat
    18361 tgcaggaata tccgcagtaa aatcttccga agccggactg ggcgcgctca gcgccacatc
    18421 cggctttggc aaactacaaa tccaacacca ggcgtttacc cttcgcacgc gaacaacaga
    18481 tcaacatact ttgctggctg gcacgctctt catcgctaaa atactgatcc cgatggtcag
    18541 cttcaccttc cagtattgcc gtttcgcagg ttccgcatac cccttcacga cataaacatt
    18601 ccactttcgc ggctttatta ttttcaataa cctgcaaaat agtcatctct tccggcacca
    18661 caaactcttt tccggaacgg gcaagcacca gggtaaatgc atcgccggtt ttgtcttcga
    18721 tagcaaattg ctcaaagtgc agtgtatcgg cggcgatgtc cagacgcgca gcttcacttc
    18781 ttaccgcttc aattagcgcc tcggggccac aggtataaac gtgtgtgcca ggttcgatat
    18841 ccgccaataa tcgcgctaat tccagtcgtg ttccggttga tgaatgatgc aaatggactt
    18901 tctcagcctg cggatgctgg actagctcag cacgaaatgc gcaactttct ggatttcgcg
    18961 agcagtaatg tagctgccag tcgatgtcgc tgtgttgcaa ctctgccatg tgcgacagga
    19021 aaggggtgat accaataccg cccgcgataa acagatgctt tctggctgag ggaattagcg
    19081 caaacaggtt attaggcgtt gagatcgtta accgatcgcc cacttttacc tgctgatgca
    19141 aaaagcggga accgccgcgc gagttttctt ccagtcgaac ggcaatctga taactagagg
    19201 tgttatgcgg tgaactcagt agtgaatacg cattgctgta ctggttatca ccatcgctca
    19261 tctgcactag gatgtgactt cctccggtga acgcaggcag cggtttgcca tcggttgcca
    19321 ccagcgtgaa gcgtttcacc tgttcggtaa ggggttcaac ctggctcacc tgtacttcaa
    19381 acatttgata gtctgacatt gctgtactcc atcaaaaatc agcccagcgg cggaaaatac
    19441 cgccgccgat gtcattagtc cttaaacacc tgcgccagca gattatggaa atgggcgata
    19501 ccatgttcgg aaatgccact accgctactg tcggccatga tgcgcccctg accacgatag
    19561 ccacgcgatt tcagcccttt ctgtacgctt tcaaccagac gtaaatcttc cggatggaac
    19621 acatcgcgat accactcaat cagcgatttt tgctcgtcgg ttaactcttc attggtgaag
    19681 taaatatcgt agttttgcag ggtagtttca gaatccaccg ggaattcata aatgaccgtc
    19741 atcatccctt tgatcggggt gacgttcagc atcgtgcacg gccacagcca gaaaccgtgg
    19801 aatgccgcat ccgtaccctc ttcaaattta aacgactgtt cggacggttt ggcgaaaccg
    19861 tattgcagcg tccagttacc gtgcatggtg tgccagtaac gatcaacctg tacggagtcg
    19921 gagaaaccta gatgcgccgg accacagtga tagcactcga gatagttatc gacaatgttc
    19981 ttccagttgg caggcgtgcg ggtggtaaag cgggccgcta gtttcagatt gtggacttcc
    20041 gggcaggctt ccagcacttt cgcgcccaga ccggtgtatt gattcacttg aagtacgaaa
    20101 aaaaaccggg aggacattgg attattcggg ttctgatggg attagatttg gtggggcttg
    20161 caagcctgta gcgcaaattt tagtcgttaa tcaatgaaac gcgaaagata gtaaaaaatt
    20221 gcttttgttt cattgaaaat acgaaaaaca aaaacactgc aaatcatttc aataacagct
    20281 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtaacc aaatgactca tatcacaaat
    20341 cagctcatgc cgtttaggta tgttacatgt gtgattatgt gaggtgaagt atgttttagc
    20401 tggttcatgg ttgttatacg gcttttttta catcttgtgg ttcttgtgaa ggagctacaa
    20461 gaccttcctg ttcataaata ccatactttg acaaaatctt tttacgagta tctgttggta
    20521 atgattcaag cattttggct gtttgtaagg tttgctgcga aataccttgc aaatcagcct
    20581 ggatggaatc atcggaagaa aaaatctcag gcataagagc ttcaaatcct ttctttctga
    20641 acaactcaat cgcagaatgc agttccttgt cctctaacaa ttcgacaatt gcgatcagtt
    20701 tagttttcaa tgtgctgcgg tcaggttgag aatttttatt ctcttgatta tttttatgct
    20761 caaccattgg catgctctga ctttggataa accactctat tggacgccct gttgcgtagg
    20821 ctatttgtgc taatcgatct aaagacgggt atgtcttgcc agacaggtag tttctgataa
    20881 ccccatcaga aagtccgcac ttagctgcaa acgcccggct tgattctcca ttcattgcct
    20941 cttcaaggcg ttccttgaaa cgcattattt ccgggggcat aaaaatgcgc tctgcatttt
    21001 tgctttcttt ttcacctgtc atgcccaatc tctctgttat ataaagaatt ttacagaatt
    21061 ctttgatttg cactg
//
LOCUS       DAJTNP010000067        20769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTNP010000067 DAJTNP010000000
VERSION     DAJTNP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..569
                     /locus_tag="OO160_002677"
     CDS             <1..569
                     /locus_tag="OO160_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5524739.1"
                     /translation="NAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQR
                     AKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHA
                     QKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDF
                     SHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            587..1225
                     /locus_tag="OO160_002678"
     CDS             587..1225
                     /locus_tag="OO160_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5524740.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1516..2244
                     /gene="mpaA"
                     /locus_tag="OO160_002679"
     CDS             1516..2244
                     /gene="mpaA"
                     /locus_tag="OO160_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5524741.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO160_002680"
     CDS             complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO160_002680"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5524742.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3303..3809
                     /gene="tpx"
                     /locus_tag="OO160_002681"
     CDS             3303..3809
                     /gene="tpx"
                     /locus_tag="OO160_002681"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5524743.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO160_002682"
     CDS             complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO160_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5524744.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5542..6603)
                     /locus_tag="OO160_002683"
     CDS             complement(5542..6603)
                     /locus_tag="OO160_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5524745.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6600..7997)
                     /locus_tag="OO160_002684"
     CDS             complement(6600..7997)
                     /locus_tag="OO160_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5524746.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8153..9151
                     /locus_tag="OO160_002685"
     CDS             8153..9151
                     /locus_tag="OO160_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5524747.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9262..10134)
                     /gene="ompG"
                     /locus_tag="OO160_002686"
                     /pseudo
     CDS             complement(9262..10134)
                     /gene="ompG"
                     /locus_tag="OO160_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10207..11288)
                     /locus_tag="OO160_002687"
                     /pseudo
     CDS             complement(10207..11288)
                     /locus_tag="OO160_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11302..11961)
                     /gene="pgmB"
                     /locus_tag="OO160_002688"
     CDS             complement(11302..11961)
                     /gene="pgmB"
                     /locus_tag="OO160_002688"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5524748.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11958..14225)
                     /gene="ycjT"
                     /locus_tag="OO160_002689"
     CDS             complement(11958..14225)
                     /gene="ycjT"
                     /locus_tag="OO160_002689"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5524749.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14222..14845)
                     /locus_tag="OO160_002690"
     CDS             complement(14222..14845)
                     /locus_tag="OO160_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5524750.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14842..15264)
                     /locus_tag="OO160_002691"
     CDS             complement(14842..15264)
                     /locus_tag="OO160_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5524751.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15286..16061)
                     /locus_tag="OO160_002692"
                     /pseudo
     CDS             complement(15286..16061)
                     /locus_tag="OO160_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16079..17131)
                     /locus_tag="OO160_002693"
     CDS             complement(16079..17131)
                     /locus_tag="OO160_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5524752.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17162..18004)
                     /locus_tag="OO160_002694"
     CDS             complement(17162..18004)
                     /locus_tag="OO160_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5524753.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17991..18872)
                     /locus_tag="OO160_002695"
     CDS             complement(17991..18872)
                     /locus_tag="OO160_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5524754.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18893..20176)
                     /locus_tag="OO160_002696"
                     /pseudo
     CDS             complement(18893..20176)
                     /locus_tag="OO160_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20190..>20769)
                     /gene="ycjM"
                     /locus_tag="OO160_002697"
     CDS             complement(20190..>20769)
                     /gene="ycjM"
                     /locus_tag="OO160_002697"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5524755.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5068 a         5665 c         5050 g         4986 t
ORIGIN      
        1 ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg ggtgttaata
       61 tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta ttgaatgcaa
      121 tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac gaattacttc
      181 ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt tatgaagcaa
      241 ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa atgttgttct
      301 cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg ttactgaccc
      361 agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat cgtgatggac
      421 tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg ttacccgatt
      481 tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg gcaaaagtta
      541 ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga aaaatgtact
      601 gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg cagataaaca
      661 aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac cctatccaac
      721 taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc aggccatgca
      781 gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc aggcaaattc
      841 agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc tttccctcgg
      901 tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta ttggtgaacc
      961 cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg aatatacaat
     1021 tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta cctctcgcaa
     1081 tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa ttacagatat
     1141 catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac ccgggactga
     1201 tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac tgtactatgt
     1261 gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga ttaacatgtg
     1321 tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc taatatctgc
     1381 acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg atgacatagc
     1441 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
     1501 gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc atttccgccc
     1561 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tcctgcccct
     1621 gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
     1681 gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg ccatcatgtg
     1741 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
     1801 gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg
     1861 aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc tggctccgaa
     1921 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg ggtggtctca
     1981 ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg cgaatggctg
     2041 gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc tggctctttc
     2101 ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc gcccatctcc
     2161 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
     2221 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
     2281 tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
     2341 cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca cgcgcttcag
     2401 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg ttaaccatct
     2461 catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca tcagcacaaa
     2521 tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga agcggttgtt
     2581 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
     2641 gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg cgaattgcca
     2701 ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
     2761 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca accgtctgtg
     2821 cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
     2881 ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
     2941 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg acgctcataa
     3001 tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg cattcgccgg
     3061 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc
     3121 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc ttaacgattc
     3181 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
     3241 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
     3301 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
     3361 aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct gacgtcaccc
     3421 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggtg
     3481 tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac aacaccgttg
     3541 tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
     3601 tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa gcctacggtg
     3661 tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
     3721 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
     3781 aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt gcaccatcag
     3841 gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc gcaacttatt
     3901 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
     3961 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
     4021 ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca acaaaatatc
     4081 ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag cgttctttaa
     4141 ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt cagcggccag
     4201 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
     4261 cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
     4321 ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat tcttctgcgt
     4381 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
     4441 gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
     4501 atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc cttccggagc
     4561 atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat tcagcgccag
     4621 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa gatctttacc
     4681 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
     4741 ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg cgctgacgtc
     4801 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
     4861 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
     4921 caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg gttcgctttc
     4981 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
     5041 atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
     5101 catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca acgccagatg
     5161 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
     5221 tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
     5281 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
     5341 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcatgggaac
     5401 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
     5461 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
     5521 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagagtttc
     5581 tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt catcgtcaat
     5641 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg tcagaagtcc
     5701 agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
     5761 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
     5821 cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca gcgtggcaat
     5881 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
     5941 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
     6001 ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac gatcgttctg
     6061 cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat caatacccgc
     6121 ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc tatgcaataa
     6181 atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
     6241 tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
     6301 cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct gcccgacaac
     6361 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
     6421 acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg cttcgtcgag
     6481 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
     6541 tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
     6601 catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat atgcggcagc
     6661 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
     6721 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt gagaagcgca
     6781 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
     6841 ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat cctttcaaac
     6901 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
     6961 tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc gataaccggc
     7021 gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat cagctgcgtc
     7081 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatcc
     7141 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccact
     7201 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
     7261 tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
     7321 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
     7381 tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc cagacggttt
     7441 tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt cgccgaccat
     7501 tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa gtaatcctgc
     7561 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc cagatacagc
     7621 gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg taacgccagg
     7681 cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc atataactgc
     7741 gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg aatgcgtttt
     7801 acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc tccggcatga
     7861 atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct gcggctaagt
     7921 ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attgagttca
     7981 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aaatcattcg
     8041 taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg
     8101 ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac ctatgtcccc
     8161 tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct
     8221 gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga
     8281 attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt ttgatgccat
     8341 tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt tctctgaagt
     8401 tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat tacaaacgtc
     8461 gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga aaatgattaa
     8521 aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac tcgataaata
     8581 cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg tttattctgt
     8641 cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg aaagtgggca
     8701 tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg atcgcgttaa
     8761 tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat ggattgttga
     8821 cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa gtcagtcacc
     8881 gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt atcgtgcggc
     8941 ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata gtaatgaaac
     9001 gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc aggagttagg
     9061 tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac cacagaatat
     9121 taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg aatgccggat
     9181 gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta ctcgcgccgc
     9241 atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg cataatggaa
     9301 tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga
     9361 aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat catggccttc
     9421 acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa tgcgcgtata
     9481 cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt cttgtgtgga
     9541 aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat aatagttcac
     9601 tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt cgacacgggt
     9661 atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca accaaccgtt
     9721 gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc agcgctgcat
     9781 attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac ggaaaccgcc
     9841 ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct ccagctccgg
     9901 gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt cctgataata
     9961 ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag gctccgccag
    10021 cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg caccgatatt
    10081 aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac acatcaccag
    10141 tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa tgccttcccc
    10201 ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt tcgtcatatc
    10261 aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag cacggaccac
    10321 taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa ttctgccacg
    10381 ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc ttccgggcgt
    10441 attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc taatttctct
    10501 tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat cgtgccgcga
    10561 ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc gggttggtta
    10621 taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat cacaatccgc
    10681 gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt ggtgttcagt
    10741 ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc atcaaggtta
    10801 gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg cccgagcgcc
    10861 acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta ctcacgcagg
    10921 ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc gattttttgc
    10981 atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa cgcgtagttc
    11041 tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg tttgccgtcg
    11101 atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag ggtggtcgat
    11161 ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat ttccaggttg
    11221 aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg taacgaaagc
    11281 tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc gcgaccaggt
    11341 cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac cgatccccac
    11401 cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt caatgccgat
    11461 acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt ccggatccgg
    11521 tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac gcagctccag
    11581 cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg gaatctgctg
    11641 tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa ccgtcagctc
    11701 gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc gctcctgcgg
    11761 gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact catcgcggct
    11821 gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt cagcggcaat
    11881 ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat ccagatcgaa
    11941 aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc attgaagggc
    12001 aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag ctgaccgtcc
    12061 aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc gagagtgacc
    12121 tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg ctcaagtaac
    12181 gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc ctgaatcgcc
    12241 cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact atgcggatca
    12301 gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt cagcaggcca
    12361 cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg aatagtgcgc
    12421 ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc tggcagcatg
    12481 taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac ttctgcgcgt
    12541 gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc cagattaatc
    12601 gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg aatttctggc
    12661 agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc actacagcca
    12721 aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata gctggtgaag
    12781 gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg aatttccaga
    12841 cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag aagtagcgcc
    12901 atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg aataaccgcc
    12961 caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt ttgccgcagc
    13021 ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct gcgcgcgctt
    13081 tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg cgcgcctggc
    13141 aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct aaacagatgg
    13201 aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc ttcgccggtt
    13261 aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat gcgcagatga
    13321 taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac cgtgatacgg
    13381 cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg gtcgtaactc
    13441 tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc taaagcagct
    13501 tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt ctcgcctgca
    13561 tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata gcattgctgc
    13621 acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc atcctgggtg
    13681 gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc gtcgagatgc
    13741 tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac tgacgcgtcg
    13801 gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc tgccgaaaca
    13861 aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac aacgctacgg
    13921 cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga taacgagaaa
    13981 acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac cagttcgttg
    14041 atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg cgtctggcgc
    14101 gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg cgccatcagc
    14161 gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt tactggcctg
    14221 gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc accagttgct
    14281 ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt acgtgattga
    14341 taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt tgcatcagcg
    14401 tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc gcaccggctt
    14461 tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt gcaaatgacg
    14521 tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc gaatcttcga
    14581 cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc gtgccgatct
    14641 tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca tacatcgcag
    14701 catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt tctttattgg
    14761 taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg gtaacgtaaa
    14821 tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg agcgcaaacg
    14881 atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc acatttccca
    14941 cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc ctgcttccag
    15001 tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa ccacatcagg
    15061 tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc cgtatttttc
    15121 tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt ccagatcgtt
    15181 gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc ctattatcgc
    15241 gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc aggtacgcaa
    15301 ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac gcccttcata
    15361 caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt ggaaatcgag
    15421 ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac cgagcaagtc
    15481 acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt gatagaaatc
    15541 gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag catcggcgag
    15601 ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca cggttccggt
    15661 acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc ggtcgccgtc
    15721 caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc acgctgccgg
    15781 aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa gccattaaga
    15841 cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc ggtggtcact
    15901 ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac cagcagtttg
    15961 ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct ctcgcggata
    16021 ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt cctttgaatt
    16081 aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac tgcatgtagc
    16141 tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa ttgagataac
    16201 cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag cgcggataat
    16261 ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg aaatgcgctt
    16321 cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag gagatggtgc
    16381 cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga
    16441 taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct acgtcagtgc
    16501 caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag cgatgggcga
    16561 taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg atggcaattt
    16621 gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg ttggcatcgc
    16681 gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc caggagctgc
    16741 cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc gtctcggaga
    16801 gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg ctgccgcact
    16861 cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca atgccgcgcg
    16921 gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct tcatcgataa
    16981 acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca
    17041 cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc agcgcagcaa
    17101 cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga aactggccgc
    17161 tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc aaaagcgcat
    17221 acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt ccccagatgt
    17281 aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg ctggcgctgg
    17341 agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag tagacgaaca
    17401 ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt tgcagacggt
    17461 tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg aagtaacttt
    17521 tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc agcgccatct
    17581 cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc agcaaaatgc
    17641 cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct ttaaagcgca
    17701 ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg gaggaaacca
    17761 ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa
    17821 tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc agcgatatcg
    17881 cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac agcgtgatga
    17941 tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt ttatttgttg
    18001 ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat gatgaagagc
    18061 accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt tttgtagaga
    18121 tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa catgtagaac
    18181 atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc gggcagcgtg
    18241 acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc gtacagcgat
    18301 ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa gtagcgccag
    18361 atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca cagcggtgcc
    18421 tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta gccgttgttg
    18481 aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga gaggatcacc
    18541 agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc cacggccagc
    18601 ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat ccacagcgag
    18661 tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac aaacgttgac
    18721 tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga caccatcggc
    18781 cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag cgcaaacggc
    18841 atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg gtttagtgct
    18901 gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg agatcggctt
    18961 tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc acatcgccca
    19021 tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc agttcaccga
    19081 ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc caggtggcgg
    19141 tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc acccagtcgg
    19201 caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg gtcttttgtc
    19261 cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc tcggttggca
    19321 ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg atataggtgg
    19381 agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc atgatgtcgt
    19441 tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc tgcatcatct
    19501 ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag ttggataacg
    19561 caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc gcaataccgt
    19621 attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt tgccagtttt
    19681 tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg ccgccaatcc
    19741 aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca atacgcagta
    19801 cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc gctttgcgat
    19861 cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg atcacttccg
    19921 gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct tcttccacgg
    19981 gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc aatttgctga
    20041 taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat acatgggttt
    20101 tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat aacagcacga
    20161 tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg ccaggggcgc
    20221 agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc atgcagatca
    20281 gtaatattta catgctgaat atttttactg acattaaaca atccagtcag acaattacca
    20341 tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat ggtaaaatta
    20401 ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga caactcatga
    20461 tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc ttcactgtga
    20521 tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc gtaatcatta
    20581 cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact taacaaaatc
    20641 gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg gcggcttaac
    20701 gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg attatttttc
    20761 cagtttact
//
LOCUS       DAJTNP010000068        20709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTNP010000068 DAJTNP010000000
VERSION     DAJTNP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO160_002698"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO160_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5524756.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO160_002699"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO160_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5524757.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO160_002700"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO160_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5524758.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO160_002701"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO160_002701"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5524759.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OO160_002702"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OO160_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5524760.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OO160_002703"
     CDS             complement(8218..9111)
                     /locus_tag="OO160_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5524761.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO160_002704"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO160_002704"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5524762.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO160_002705"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO160_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5524763.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OO160_002706"
     CDS             complement(10242..11474)
                     /locus_tag="OO160_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5524764.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO160_002707"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO160_002707"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5524765.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO160_002708"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO160_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5524766.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="OO160_002709"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="OO160_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO160_002710"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO160_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5524767.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO160_002711"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO160_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5524768.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO160_002712"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO160_002712"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5524769.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO160_002713"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO160_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5524770.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4982 a         5727 c         5029 g         4971 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatca ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaat
//
LOCUS       DAJTNP010000069        20685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTNP010000069 DAJTNP010000000
VERSION     DAJTNP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(138..566)
                     /gene="mxiM"
                     /locus_tag="OO160_002714"
     CDS             complement(138..566)
                     /gene="mxiM"
                     /locus_tag="OO160_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5524771.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(550..957)
                     /gene="mxiM"
                     /locus_tag="OO160_002715"
     CDS             complement(550..957)
                     /gene="mxiM"
                     /locus_tag="OO160_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5524772.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(950..1645)
                     /gene="mxiN"
                     /locus_tag="OO160_002716"
     CDS             complement(950..1645)
                     /gene="mxiN"
                     /locus_tag="OO160_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5524773.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1587..2114)
                     /locus_tag="OO160_002717"
     CDS             complement(1587..2114)
                     /locus_tag="OO160_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5524774.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(2111..2836)
                     /gene="mxiJ"
                     /locus_tag="OO160_002718"
     CDS             complement(2111..2836)
                     /gene="mxiJ"
                     /locus_tag="OO160_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5524775.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2842..3135)
                     /gene="mxiI"
                     /locus_tag="OO160_002719"
     CDS             complement(2842..3135)
                     /gene="mxiI"
                     /locus_tag="OO160_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5524776.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(3148..3399)
                     /gene="mxiH"
                     /locus_tag="OO160_002720"
     CDS             complement(3148..3399)
                     /gene="mxiH"
                     /locus_tag="OO160_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5524777.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(3407..4522)
                     /gene="mxiG"
                     /locus_tag="OO160_002721"
     CDS             complement(3407..4522)
                     /gene="mxiG"
                     /locus_tag="OO160_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5524778.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(4531..4989)
                     /gene="ipgF"
                     /locus_tag="OO160_002722"
     CDS             complement(4531..4989)
                     /gene="ipgF"
                     /locus_tag="OO160_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5524779.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(4989..5351)
                     /gene="ipgE"
                     /locus_tag="OO160_002723"
     CDS             complement(4989..5351)
                     /gene="ipgE"
                     /locus_tag="OO160_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5524780.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(5361..6977)
                     /locus_tag="OO160_002724"
     CDS             complement(5361..6977)
                     /locus_tag="OO160_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5524781.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            7291..8775
                     /gene="icsB"
                     /locus_tag="OO160_002725"
     CDS             7291..8775
                     /gene="icsB"
                     /locus_tag="OO160_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5524782.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            8788..9177
                     /gene="ipgA"
                     /locus_tag="OO160_002726"
     CDS             8788..9177
                     /gene="ipgA"
                     /locus_tag="OO160_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5524783.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            9193..9819
                     /gene="ipgB1"
                     /locus_tag="OO160_002727"
     CDS             9193..9819
                     /gene="ipgB1"
                     /locus_tag="OO160_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5524784.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            9876..10343
                     /gene="ipgC"
                     /locus_tag="OO160_002728"
     CDS             9876..10343
                     /gene="ipgC"
                     /locus_tag="OO160_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5524785.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            10349..12091
                     /gene="ipaB"
                     /locus_tag="OO160_002729"
     CDS             10349..12091
                     /gene="ipaB"
                     /locus_tag="OO160_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5524786.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            12111..13202
                     /gene="ipaC"
                     /locus_tag="OO160_002730"
     CDS             12111..13202
                     /gene="ipaC"
                     /locus_tag="OO160_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5524787.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            13253..14251
                     /gene="ipaD"
                     /locus_tag="OO160_002731"
     CDS             13253..14251
                     /gene="ipaD"
                     /locus_tag="OO160_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5524788.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            14260..16161
                     /gene="ipaA"
                     /locus_tag="OO160_002732"
     CDS             14260..16161
                     /gene="ipaA"
                     /locus_tag="OO160_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5524789.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            16165..16401
                     /locus_tag="OO160_002733"
     CDS             16165..16401
                     /locus_tag="OO160_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5524790.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            16618..17547
                     /gene="virB"
                     /locus_tag="OO160_002734"
     CDS             16618..17547
                     /gene="virB"
                     /locus_tag="OO160_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5524791.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(18491..19270)
                     /gene="ipaJ"
                     /locus_tag="OO160_002735"
     CDS             complement(18491..19270)
                     /gene="ipaJ"
                     /locus_tag="OO160_002735"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5524792.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(19870..20007)
                     /locus_tag="OO160_002736"
     CDS             complement(19870..20007)
                     /locus_tag="OO160_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524793.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT         7078 a         4216 c         3061 g         6330 t
ORIGIN      
        1 ccttccatgc tggagccagc tcaattatgt tacgttcttt atcttttaca agctcaacgt
       61 taactggttc atgtccacta gagtaaatgt aaaacatatt acttgtattt agacctttat
      121 atttactcac aatacaatta tatccctcga attaatttta atttaacttc cccatccttc
      181 tctataagga tggtatcatt tatcttatta ccttttaaaa caaccccgtc tttcagcata
      241 gaaaatagtt tatctatagc tatcttttgt gcatccatga agcacccatc tacttcctta
      301 acattaaagt tgcccatgaa tttacctgca ttaaaactat aaaccgcctt accactaata
      361 catttagagt aaacatttat acttttattg gttgtattaa acgaccataa taaatcattt
      421 ggaatagaaa aataatcctt acttacagga acaatatgcc attctttttc agaattagat
      481 gatgacttta aagcacaccc actcagtgtt aatagcaata tacttaaaat aaatattttc
      541 aacttattac taccatgtcg aatcatctgc ctctctccct gcttcaaaga cactaattaa
      601 tgaatcgaag gttgtagtct taatatagaa atcaatattg ttatatatgg acaatgatgc
      661 ataaaatccc tcatgcaaat cgacatattg atttgcatct atgagtttat taagaagtga
      721 acttttttgt gcagctgtaa gataaggaaa acgtgaattg aataattgtt caatcttatt
      781 gtgcagttca tcattatcta tggaatttaa ttcactctgt gagaaatatt gcattatttc
      841 atagattata tcctcatcaa atgattgcga agatgataat ctctcattta aattaacaac
      901 ttcttcacta tttaatcttt gctgcagagc attgcttgca tttatttgat taatcattaa
      961 caggattctc ttttaacaag tcttctgcaa tttgtctcag tttcttatgt gctaactctt
     1021 tgaaatattt tttatcattt tttgttaatt tttccgcaag ttctatttta acacctgata
     1081 ttacatcctg aggactaaac tccactactt gattaccact acagaatata taattattcc
     1141 cttcagaata ttttaaaata atcttgacat ctgagcgata tttatggaga tcaagttcta
     1201 actttttcct cagtgctaaa ttacacttgg gcaaaaccac ttttaattct gttactgtat
     1261 tacgcaacga actaagccac tgctccaata aagtgctaat gatcctctca tcatgtaaat
     1321 tttctgaaag caaactagtt atatagtcct caatatttct tctattttca ttttgcttta
     1381 acttccattc acaaatatag tcaataatat gctcaaaagc agtctgtata ccaatttgat
     1441 aaccatcaca tacagcatct atacgcagtg attcagcttt tttcgtagcc tcctttataa
     1501 cacaaattgc tttcttctta ctctcagtgt ctctatcttt tatggtttta cataattcct
     1561 tttcaattct ctttataaca acaccatcat aggcatgatg tctggatacc ggtaaagtgc
     1621 ctttttgcat attgcatacc ttcatttaat cgggacaaat ttctttctac aggcgtataa
     1681 tttccctcca aagcaattgg attaaataat aagtttattc tttcatatat accaacagat
     1741 atattttcct tccacgcaat aagatgccaa aaaccacttc gaatagcggc ctccccttgc
     1801 ttactgattt tgcccccata ataactaata accatctcac tatgattaat taaccgagac
     1861 tcttgactac cgataaaata agcaacagct ggaagtaaat tccagttggc aacaaacata
     1921 gcggttaccc cgcttaaagg ttcaatatcg caatttagat catatttatt aattatgaga
     1981 ttattaatct cacttctgat tacgccattt tctaacagtt cagaaggcaa attcaaccga
     2041 tttctattta tatagaacgc tggatcaaaa attattgaaa gatatttttt ataaattcca
     2101 tccattctta tcatattttg tttctcttga accaccctgt tttgaatgcc cataccaaaa
     2161 gaatgacaac tagtacagcc atcccgagaa ataaatatat tacttcattt gttaaaaatt
     2221 ccgatttaac ttccttaaca ggttgtacat ttgtataaac atattcttct ttcggagtta
     2281 atatgacaga tatattttca tacttaacat cagaaaaggt gtttttcaaa aatcgcttaa
     2341 tattactaac taatagttca gactctttcg gtgagtcata tatagcgatt actgatatat
     2401 gcatcggttt tgaagatata tttttttctt caagatcata gctaacatgt atttttgccg
     2461 aaataacacc accaatagaa actaaagact gttccagccg ttgctcaata gcactatata
     2521 aacgggcctt ttcagctctt ggagaagaca ctaatgaatc tgtaggaaac atttgtgaga
     2581 tatcaactct ctccggattt ggcaaatcgt acatgcgcat caaatcaact gccgatgcaa
     2641 atgtcccctt ttcgacttgt accgagatcc cctgtttacc tccatcaact tttctagcag
     2701 taatattatg gcgttctagc acagatatta tttcatttgc ctgtctttga gataaattag
     2761 aaattaactc ttcacgttgc tcacatccaa tcaacatcag caacaagaat aaaataaaac
     2821 ctttatacct aatcatataa attaagactt taataaagtt tcaacagccg aaactgtttt
     2881 tcttgctaac gtgccagcta atgatactcc tattgaataa tttgagagcg tcgtctgtaa
     2941 cttggccaaa gattctgggt ttaatgattc tggattgctt accatctcca ttatttgtga
     3001 tacttgaata tctgtatcca tcttaatatc actatattta gccgacacga cgtcttccag
     3061 acttgatatc tcttgagatt gaaaatcact ggctttgata atatcaacct gattgactgg
     3121 ataaatgtaa ttcatgaatg ctccctatta tctgaagttt tgaataattg cagcatcaac
     3181 atccttaatc actttcactg tattggattg cgcgttccta tataatgtat attcagataa
     3241 tttactttgg tattcagcca gcaactgtgg attcgaagga tttttagcta atttatctag
     3301 tgccaatgtt agttcacctt gtaatgtttg agttccatca tcaaaagttt cagataatga
     3361 actcaatgtc caatcatcat tcggtactgt aacactcatt ttatcctcac ttatttttat
     3421 ctaaaaaaaa ccagtgttta tcattcaaca ttacataact gtctttgctg ttaagatatg
     3481 atttaccttt aaaatcatca tctatcaaaa gtacagaaaa ctcaatatac tgatctccat
     3541 atatattttt atgttcagaa atcagtctat taatgagctc aagcttttca tctgtcagtt
     3601 cttctctgac agaatatgta actttattat tttcacaaat ctctttatac tggacattta
     3661 cctttgtgaa aataccttta gcatcccccc ttgcattatg atcgctaaga tagttgaatt
     3721 taatattttt gatatacgga aattcaacaa tcagagcatt tttcaattta tcaagttcgg
     3781 tatcttttga actatttctt tctttactta aaaacaccag taattccgct ttatcactca
     3841 ctaaattcaa cacatacaaa ttgataaatg ggaatcgttg attgatatat tgttgaattc
     3901 tatttatttc tttgtttgac accagaatat atctgcattt ggtaaatccg gttttattta
     3961 aggctacaga cgcccatacc gcatcatttt gtgtttcggc aagaatataa ttgcactgtc
     4021 cagtgttgac tattccatat ctcttatcat ctataatttc agcaatttct tttacttcat
     4081 ctttctttaa agagagggaa aaaattataa ttaacacaac aataacagca aagaaaaaaa
     4141 acaccgagtg gttcttatac attccgttta gtatatggcc tctcgattta tcctccctca
     4201 tatttttaag gtgaaaagaa atcccgtcat attcaaatga attaaattgt gaattgatat
     4261 caatttgctc acctttttca tcactaaatt gacagatatt atctttgctt atggtcaaat
     4321 aaattatacc atccgattta ctatctgtta caggaataat attttctggg aaattgtcgt
     4381 ttccaaactc aagtgtttct atcgtccgtc cgagaacaat taagttattc ccataatata
     4441 acggaaactc atccccgaca ccgtttgtga gtttcactaa taacctgaat ggtgcaagat
     4501 ttgagttctt tgcctcagac ataaatcctc ttatatcctt cgattattct gcttgctctc
     4561 tgctgctatc cgcaaatagc gtctatagat atcctctgcg tactttaatc tttctttttt
     4621 ctttttcgga gacgttcctg cattataggc tccaacagcc tcccaccctc ttccatacat
     4681 catcataaac tcatttaata actttgctgc ataatgtaca gaaagacatg gatgactaat
     4741 tagcatttcc tcagaatatc ccatttctct aagtctttta gaatgaaagt catttatttg
     4801 cattatacca taatctttac ttccattgtt attaacattg actgcagatt tattaaatcc
     4861 ggattctttt tccgcaatcg cttttaacaa acttgatgga atattgtacc tttcaccagc
     4921 cttatcccaa caatcagctc tccctaaata tgggataaaa cacagcaaaa taaatacgaa
     4981 acgggacatt aatacccctt cattcttcgc gcaaattcat ccttcagcca acgcacacgc
     5041 gaaatataat attctgtatt aacatataca tcctcgaatt catttatccc ggtaagatct
     5101 aatcgcgcaa ttagatttcc gccttcatca tcagtagcta agcatatctt ttctgagtaa
     5161 tttaggctca acgcatatat aagatcattt atattttcag gcaatgcaca aaaagggcat
     5221 aataaattta ttgaatctcc ttctttttca atatatatta gcacatcatc atcaagcatg
     5281 attgcttgat catcgatatc aattaaaggg atacccaagg ctcggcaaat aacatctgct
     5341 aaatcttcca tatattcctc ttatacaaat gacgaatacc ctttcaccat attccatatt
     5401 tttgggtccc ctattctttc agaataagat agctctaacg aagatagcgg caattttttc
     5461 atgactttat ttccgggcac accagtattc atctcttgga tttccatatt accactattc
     5521 attagaatag tagaaaataa tcttttctct tctgaggata atttactatt taattgagaa
     5581 aactgacctg tttcatgttt tcttattatt tctcttttaa tttcagcatc ctgcatacct
     5641 gttctgtcct tcccactctt acaattccag caaggtacag cacctatagt ataagccaat
     5701 agagtcattc tttgtgacaa tttatacggc tctccattat catttttttg taactttttg
     5761 gttacaatct cttttatctg gttagccaga tatatcacat cattcttaca tggtggattt
     5821 ttttctattg cttctgcagc ccaacctcca atcacaccat ttttgaggaa attatccccc
     5881 aacaaagaac atattgattc atcattaagc ttgtcaacat tcctccagcc taagcccatt
     5941 tttagcgcta attcatttac accaaagtta aatgtcacca cttttagatt aacgcttact
     6001 tctttcagaa gaccatcact attccgaatt aataatttcg ttggcttccc ccttttagag
     6061 tttaaccctt ttaatgcgtt cacctgatct tttaacatac tctcctctcc cccggttaaa
     6121 ctggtcggcg tcagaagaga agtcgaaaca atctttaaat ctactgtttt accagacaaa
     6181 gcctgtgata ataactctgg tctgctatat aatgctgcgc ttactaactc ctcagcttta
     6241 ttacgagcag caacagctct ttcagatgag ttttttttca atccataggc tgatataact
     6301 ccatgtctga tacccgaaaa aatttccttc ccctcatcat ccaccacttt tgaaagccac
     6361 atattcgcga tatgatcact ctctctcgta gaagcacaac aaattccttt tccattatac
     6421 tctttgacaa atatattttt attccctatc ttcatatgag aggcaggagt taattgaaaa
     6481 ccataatttt tcccatgatg actaatattc ttgtcgaccg gtccccaatt cttattattt
     6541 aaataatcag taaatatttg ttttattttc gtttgcaatt cctttttatt tttaatgcct
     6601 agctgtgctc ttatctcttc acttattact ttattaagct cattaatatg tcttttatta
     6661 acttcaccaa gtttataggt atctaatcgt tccatcatgg ctttaatttt tgacgtatca
     6721 ccacctgcca actttattgc ctcgcaaaaa actccagctc tgtcacgcgc aaacaaaagg
     6781 ctttttcctg ttaagcttgt ttggttctgt aaatataatc gattaagcgc ttcgatcccc
     6841 ttatttcttt ctccattttt aactctttga tatgaaatca cgcttacacc tttgtgcttt
     6901 ccggtttctt ctgcgccttt ataggaatct ccgctttgaa atgaaacctg atgcaatccc
     6961 aaattagtta tgtgcattta attatcctca gtcataaatg gggcattgat tgcagttttg
     7021 tacctcgttt tacaacaaaa aagatactta ttcaacaggg ttctctcttt ttctataaca
     7081 ctccgagcct gatacatcag gcactttatc ttgtgggatt tcatgatgaa acgagcacta
     7141 catatgctca cgaggtacat attatgtaca gcaaaagaaa atttgattat gttaacaggc
     7201 tcaagtttct aaatttatta attgaatata ttatatacca aatacatttt acatccccac
     7261 aatcaccaag taatggagag ttaataaagt atgatcctca aaattagcaa tttcattgac
     7321 gcaagcaata caaaagggcc tatacgcgtt gaagatacag agcatggacc tatattgata
     7381 gcacagaaat tcaacctaaa ggatctcttt ttcagaacat taagcactat taatgcaaag
     7441 ataaattcac agatattaaa tgaacaacta aaaaattatc gactagagaa tcaaaaatcc
     7501 ttattgcttt tcctcaatac attagctagc gaaaaatctg cagaaagtgc gtttgccgcc
     7561 tatgaagcag ctaaaaactc aattcaacac tctttcacag gtagagacat caaattaatg
     7621 ttgaataccg cagagcgttt tcatggcatc ggtacagcca aaaatcttga aagacattta
     7681 gtttttcgtt gctggggaaa tagaggcata acccatttag ggcatactag tatcagtata
     7741 aaaaacaatt tactacagga accaactcat acctatctca gctggtatcc cggcgggaat
     7801 gttacaaaag atacagaaat aaactacctt tttgaaaaac gctcaggtta cagtgtcgat
     7861 acctataagc aagacaaact aaatatgatt tcagaccaaa cagccgaaag acttgatgca
     7921 ggccaagagg ttagaaactt attaaattca aaacaagatc aaaataacaa caaaaaaata
     7981 ttttttccca gagctaatca aaaaaaagac ccctatggtt attggggggt cagtgctgat
     8041 aaagtataca ttccattatc aggtgataat aaaacaaagg atggtaaaat cagccataac
     8101 ctttttggcc ttgatgaaac aaacatgtca aaatttatat gcaaaaagaa agcagatgct
     8161 ttcagacaat tggcaaatta taaattaatc agtaaatctg aaaactgtgc tggtatggca
     8221 cttaatgttc ttaaagcagg aaattctgaa atatactttc cactccctga cgttaaattg
     8281 gttgctacac ctaacgatgt atacgcgtat gcaaataaag ttaggcaacg cattgaaagt
     8341 ctcaatcagt cttataacga aataatgaag tatattgaat ctgattttga tctttccaga
     8401 ttgactcaat tacgacgcag ctatcttaaa agtttcaata aaattaacct tatccacaca
     8461 ccaaaaacat tcaagccttt atcaatttca ctttacaaac atcctactga aaatgtatct
     8521 tcagaagact ttgatgcggt catcaacgct tgccactcat acttagtaaa atcagcaccc
     8581 agcaatatga ctcgtgtatt aaacgaatta aaaaccgaag caacagataa aaaagaagaa
     8641 atcatcgaaa aatcaataaa aattattgat tattacaatt ctcttaaatc gccagatctt
     8701 ggaaccaaac tttacattca cgacttatta cagatcaata agcttttatt gaataactct
     8761 cattctaata tatagaaggc catagaaatg tgtcgcaaac tatatgataa actttatgaa
     8821 ataacaggtg caaagcttga ttttaacgat aaaaatcaag cctttatatt gctcgaagaa
     8881 caaatacctg tttgtataac agataatgat gaatacatct ttttgaccgg acttcttaat
     8941 gaacatgaac tttttacaga aaatattatt aatccagaac acatactcat ccttaactac
     9001 tctttgagta gagattatgg ttcttctatc tgtcttctcc ctgatactca tcaatgtgtt
     9061 ttgaccaaga aacactacaa aaaatatctt tcacctgatg aactcattga atctttgtat
     9121 gaatttttat tttgtattaa actaaccatt gcaaacatca cttcagaagt gaactaacat
     9181 atagggggta tcatgcaaat tctaaacaaa atacttccac aggttgaatt cgcgattcct
     9241 cgcccatcat ttgattcgtt aagtcgcaat aagcttgtaa agaaaatatt atctgttttt
     9301 aacttaaaac aacgctttcc tcagaagaat tttggatgcc cggtgaatat aaataaaatc
     9361 agggacagcg tcatagacaa aataaaagat agtaattcgg ggaatcaact attttgttgg
     9421 atgagccaag agcgaaccac ttatgtctct tcaatgataa acagaagtat agatgaaatg
     9481 gccatacaca atggagttgt tttgacctca gataacaaaa aaaacatatt tgcagctatt
     9541 gaaaaaaaat tcccggatat aaaacttgat gaaaaatcag cacaaacatc tatcagtcat
     9601 acagcactaa acgagattgc ctcatctggc ctcagagcaa agattttaaa acgctatagc
     9661 agtgacatgg atttgtttaa cacacaaatg aaggatctta caaatctagt atccagttcc
     9721 gtctacgata aaatatttaa tgaatcaaca aaagttttac aaatagaaat aagcgcagag
     9781 gttctaaagg ctgtataccg tcaaagcaat acaaattaaa tcttttatat taaatcttat
     9841 acttattaaa gtaaagcaga caaaaaggag accttatgtc tttaaatatc accgaaaatg
     9901 aaagcatctc tactgcagta attgatgcaa ttaactctgg cgctacactg aaagatatta
     9961 atgcaattcc tgatgatatg atggatgaca tttattcata tgcttatgac ttttacaaca
    10021 aaggaagaat agaggaagct gaagttttct tcaggttttt atgtatatac gacttttaca
    10081 atgtagacta cattatggga ctcgcagcta tttatcagat aaaagaacag ttccaacaag
    10141 cagcagacct ttatgctgtc gcttttgcat taggaaaaaa tgactataca ccagtattcc
    10201 atactggaca atgccagctt cggttgaaag cccccttaaa agctaaagag tgcttcgaac
    10261 tcgtaattca acacagcaat gatgaaaaat taaaaataaa agcacaatca tacttggacg
    10321 caattcagga tatcaaggag taattattat gcataatgta agcaccacaa ccactggttt
    10381 tcctcttgcc aaaatattgg cttccactga gcttggagac aatactatcc aagctgcaaa
    10441 tgatgcagct aacaaattat tttctcttac aattgctgat cttactgcta accaaaatat
    10501 taatacaact aatgcacact caacttcaaa tatattaatc cctgaactta aagcaccaaa
    10561 gtcattaaat gcaagttccc aactaacgct tttaattgga aaccttattc aaatactcgg
    10621 tgaaaaatct ttaactgcat taacaaataa aattactgct tggaagtccc agcaacaggc
    10681 aagacagcaa aaaaacctag aattctccga taaaattaac actcttctat ctgaaactga
    10741 aggactaacc agagactatg aaaaacaaat taataaacta aaaaacgcag attctaaaat
    10801 aaaagaccta gaaaataaaa ttaaccaaat tcaaacaaga ttatccgaac tcgacccaga
    10861 gtcaccagaa aagaaaaaat taagccggga agaaatacaa ctcactatca aaaaagacgc
    10921 agcagttaaa gacaggacat tgattgagca gaaaaccctg tcaattcata gcaaacttac
    10981 agataaatca atgcaactcg aaaaagaaat agactctttt tctgcatttt caaacacagc
    11041 atctgctgaa cagctatcaa cccagcagaa atcattaacc ggacttgcca gtgttactca
    11101 attgatggca acctttattc aactagttgg aaaaaataat gaagaatctt taaaaaatga
    11161 tctggctcta ttccagtctc tccaagaatc aagaaaaact gaaatggaga gaaaatctga
    11221 tgagtatgct gctgaagtac gtaaagcaga agaactcaac agagtaatgg gttgtgttgg
    11281 gaaaatactt ggggcacttt taactatcgt tagtgttgtt gcagcagctt tttctggagg
    11341 agcctctcta gcactggcag ctgttggttt agctcttatg gttacggatg ctatagtaca
    11401 agcagcgacc ggcaattcct tcatggaaca agccctgaat ccgatcatga aagcagtcat
    11461 tgaaccctta atcaaactcc tttcagatgc atttacaaaa atgctcgaag gcttgggcgt
    11521 cgactcgaaa aaagccaaaa tgattggctc tattctgggg gcaatcgcag gcgctcttgt
    11581 cctagttgca gcagtcgttc tcgtagccac tgttggtaaa caggcagcag caaaacttgc
    11641 agaaaatatt ggcaaaataa taggtaaaac cctcacagac cttataccaa agtttctcaa
    11701 gaatttttct tctcaactgg acgatttaat cactaatgct gttgccagat taaataaatt
    11761 tcttggtgca gcgggtgatg aagtaatatc caaacaaatt atttccaccc atttaaacca
    11821 agcagtttta ttaggagaaa gtgttaactc tgccacacaa gcgggaggaa gtgtcgcttc
    11881 tgctgttttc cagaacagcg cgtcgacaaa tctagcagac ctgacattat cgaaatatca
    11941 agttgaacaa ctgtcaaaat atatcagtga agcaatagaa aaattcggcc aattgcagga
    12001 agtaattgca gatctattag cctcaatgtc caactctcag gctaatagaa ctgatgttgc
    12061 aaaagcaatt ttgcaacaaa ctactgcttg atacaaataa ggagaatgtt atggaaattc
    12121 aaaacacaaa accaacccag attttatata cagatatatc cacaaaacaa actcaaagtt
    12181 cttccgaaac acaaaaatca caaaattatc agcagattgc agcgcatatt ccacttaatg
    12241 tcggtaaaaa tcccgtatta acaaccacat taaatgatga tcaactttta aagttatcag
    12301 agcaggttca gcatgattca gaaatcattg ctcgccttac tgacaaaaag atgaaagatc
    12361 tttcagagat gagtcacacc cttactccag agaacactct ggatatttcc agtctttctt
    12421 ctaatgctgt ttctttaatt attagtgtag ccgttctact ttctgctctc cgcactgcag
    12481 aaactaaatt gggctctcaa ttgtcattga ttgcgttcga tgctacaaaa tcagctgcag
    12541 agaacattgt tcggcaaggc ctggcagccc tatcatcaag cattactgga gcagtcacac
    12601 aagtaggtat aacgggtatc ggtgccaaaa aaacgcattc agggattagc gaccaaaaag
    12661 gagccttaag aaagaacctt gccactgctc aatctcttga aaaagagctt gcaggttcta
    12721 aattagggtt aaataaacaa atagatacaa atatcacctc accacaaact aactctagca
    12781 caaaattttt aggtaaaaat aaactggcgc cagataatat atccctgtca actgaacata
    12841 aaacttctct tagttctccc gatatttctt tgcaggataa aattgacacc cagagaagaa
    12901 cttacgagct caataccctt tctgcgcagc aaaaacaaaa cattggccgt gcaacaatgg
    12961 aaacatcagc cgttgctggt aatatatcca catcaggagg gcgttatgca tctgctcttg
    13021 aagaagaaga acaactaatc agtcaggcca gcagtaaaca agcagaggaa gcatcccaag
    13081 tatctaaaga agcatcccaa gcgacaaatc aattaataca aaaattattg aatataattg
    13141 acagcatcaa ccaatcaaag aattcgacag ccagtcagat tgctggtaac attcgagctt
    13201 aatatatcca agagccataa taatatatgg ctcttcctgt aaggaaataa ccatgaatat
    13261 aacaactctg actaatagta tttccacctc atcattcagt ccaaacaata ccaacggttc
    13321 atcaaccgaa acagttaatt ctgatataaa aacaacgacc agttctcatc ctgtaagttc
    13381 ccttactatg ctcaacgaca cccttcataa tatcagaaca acaaatcagg cattaaagaa
    13441 agagctttca caaaaaacgt tgactaaaac atcgctagaa gaaatagcat tacattcatc
    13501 tcagattagc atggatgtaa ataaatccgc tcaactatta gatattcttt ccaggcacga
    13561 atatccaatt aataaagacg caagagaatt attacattca gctccgaaag aagccgagct
    13621 tgatggagat caaatgatat ctcatagaga actgtgggct aaaattgcaa actccatcaa
    13681 tgatattaat gaacagtatc tgaaagtata tgaacatgcc gttagttcat atactcaaat
    13741 gtatcaagat tttagcgctg ttctttccag tcttgccggc tggatctctc ccggaggtaa
    13801 cgacggaaac tccgtgaaat tacaagtcaa ctcgcttaaa aaggcattgg aagaactcaa
    13861 ggaaaaatat aaagataaac cgctatatcc agcaaataat actgttagtc aggaacaagc
    13921 aaataaatgg cttacagaat taggtggaac aatcggcaag gtatctcaaa aaaacggggg
    13981 atatgttgtc agtataaaca tgaccccaat agacaatatg ttaaaaagct tagataatct
    14041 aggtggaaat ggcgaggttg tgctagataa tgcaaaatat caggcatgga atgccggatt
    14101 ctctgccgaa gatgaaacaa tgaaaaataa tcttcaaact ttagttcaaa aatacagtaa
    14161 tgccaatagt atttttgata atttagtaaa ggttttgagt agtacaataa gctcatgtac
    14221 agatacagat aaactttttc tccatttctg aggtgcgcca tgcataatgt aaataatact
    14281 caagcgccaa cattcttata taaggcaact tcaccatcat caacagaata cagcgagtta
    14341 aaaagcaaaa tatccgatat ccatagttcg caaacttctc taaaaacacc agcatcagtg
    14401 tctgaaaaag aaaactttgc aacgtctttt aatcagaaat gtcttgattt tttattttct
    14461 tcctcaggga aagaagatgt gttaagaagc atttattcca actcaatgaa tgcgtatgcc
    14521 aaaagcgaga ttctcgaatt ttcaaatgtt ttgtactcct tagtacatca aaatggtctt
    14581 aattttgaaa acgaaaaggg acttcaaaaa attgtcgcac agtattcgga actaattata
    14641 aaagataaat tatcccaaga ttctgccttt ggaccatggt cggcaaagaa taagaaactc
    14701 catcaattac gacaaaacat tgagcacaga cttgcactat tagcacaaca acacacatct
    14761 ggtgaagctt tatcattggg acaaaaactc ctcaatactg aagtatcatc atttatcaag
    14821 aataatattc ttgctgaatt aaagttaagt aatgaaactg tttcatctct caaactagat
    14881 gatttagttg acgcacaggc aaaacttgcc tttgatagtt tgcgcaatca acgtaaaaat
    14941 actattgata gtaaaggatt tggtataggt aaactgtcaa gagacttaaa tacagtagcc
    15001 gtgtttcctg agctgttgag aaaagtcctt aatgatattt tagaagatat aaaagattcg
    15061 catcctatcc aagatggcct ccctacacct cccgaagata tgccagatgg cggaccaacc
    15121 cccggagcca atgagaaaac atcccaacct gtaattcact atcatataaa taatgataat
    15181 agaacttacg ataatagagt ttttgacaac agagtatatg acaatagcta tcacgagaac
    15241 ccagaaaatg atgcacagtc tcctacttct cagacaaacg atctattatc ccgtaacgga
    15301 aactcattac taaatccaca aagagcacta gttcaaaaag taacttccgt tctaccacac
    15361 tctatatcag atactgtcca gacatttgca aataattcag ctttagaaaa ggctttcaac
    15421 catactccag ataattcgga tggaataggt tcagacctgt taactacgag tagtcaagaa
    15481 agatctgcaa ataactctct ttctcgggga cacaggcctc tgaacataca gaactcttca
    15541 accacccccc ctctccaccc ggaaggagtg acaagcagta atgataactc atcagataca
    15601 actaaaagta gcgcttctct ttctcataga gtagcttcgc aaatcaataa attcaactca
    15661 aacactgatt caaaagtact tcagactgat tttttatcaa gaaatggaga cacatattta
    15721 acacgggaaa cgatatttga agcttcaaaa aaagtaacaa actccctaag taatcttata
    15781 tctctcattg gaactaaatc aggaacacaa gaacgagagt tacaggaaaa atcaaaggac
    15841 attacaaaat ccacaacaga acatagaata aacaacaaat taaaagttac agatgcaaat
    15901 ataagaaact acgtaacaga aaccaacgca gatacaattg ataaaaatca tgcgatctat
    15961 gaaaaggcaa aagaagtatc tagcgccctc agcaaggtat tgtcaaaaat tgacgatacc
    16021 tctgcagaat tacttacaga tgatatatct gatttaaaaa ataacaatga tattacagct
    16081 gaaaacaata atatatataa agcagcaaaa gatgtaacca cttccctatc aaaagtatta
    16141 aagaatatca ataaggatta aaatatgata aaagaaaaaa tattatcaat agtggccttt
    16201 tgctatggta tagcgtacag caaactatct gaagaaacta aatttattga agatctttct
    16261 gccgattctc tttctctgat tgaaatgctg gatatgatta gttttgagtt caatctacgt
    16321 atagatgaat ctacattaga acacattatt actattggag atcttatttc tgtagtcaaa
    16381 aatagtacaa aatcaatata gtaattgata agcatttttt catctatgga gctctcacat
    16441 cagagctcca caagaatatt attcttttat ccaataaaga taaattgcat caatccagct
    16501 attaaaatag taatttttaa gactaccgtt gactatcatc aacagttaca tacaatcttg
    16561 ctcacactgc atttaacttt tgtcaatata aatcaatatg aataaacagg gtgtgatatg
    16621 gtggatttgt gcaacgactt gttaagtata aaggaaggcc aaaagaaaga gtttacactc
    16681 cattctggta ataaagtttc ctttatcaaa gccaagattc ctcataaaag gatccaagat
    16741 ttaaccttcg tcaaccaaaa aacgaatgta cgcgatcaag aatccctaac agaagaatca
    16801 ttagccgata tcataaaaac tataaagcta caacaattct tccctgtaat aggaagggag
    16861 attgatggta gaattgaaat tctggatggc actcgtagaa gagcatctgc aatatatgca
    16921 ggagcagatc ttgaagttct atattcaaaa gaatatatat ctactcttga tgccagaaaa
    16981 ctagcaaacg atatacaaac agcaaaagag catagcatcc gagaacttgg tattggtctt
    17041 aattttctga aagtatcagg gatgtcctat aaagacatag ccaaaaaaga gaatctgtct
    17101 cgcgcgaaag tcactcgtgc ctttcaggca gcaagcgttc cacaggaaat aatatctcta
    17161 tttccaatcg cgtcagaact taactttaat gactacaaga tattattcaa ttattataaa
    17221 ggacttgaaa aggctaatga atctcttagt tctacactac caatattaaa ggaagaaata
    17281 aaagaccttg atacaaattt gcccccggac atatataaaa aagaaatttt aaacatcata
    17341 aagaaaagca aaaacagaaa gcaaaaccct tcgctaaaag ttgactcgtt atttatttct
    17401 aaagacaaac ggacttacat aaaaagaaaa gagaataaaa caaacagaac tctaatattt
    17461 acattatcta aaataaacaa aacagttcag agagaaatag atgaagctat tcgggatata
    17521 atttctcgcc atctatcgtc ttcataaaca tcgaacactg atgtaaactg cccaattcag
    17581 ataataaaaa tcttcgttga acttgacttt cagccggtgg ggtaattccg tcggctatct
    17641 gttattcaat ccccacgcac tgtgtttcct taaaattacg gtgaaaactc ataccctgct
    17701 gaccacgaga aaattatctg gtgaaattag taatattcat atcattgaaa atgaattgtg
    17761 caactaaggg atgcggcatg atggtcaaaa ttttatgtga atatgagtga ctggccgaaa
    17821 atattcagcg tatttttaat cgtttcaata aaaacagcga ggattagcag tcaacgcttt
    17881 tactactcaa ttttttttac atggtgataa acaaaaaatc caccattaac agacttcgtt
    17941 cacagtatca aaaaagcttt aagaatatat tagcagattt ctcaaatata attactgttt
    18001 tattctccta atcttcccat aaaatgaccg acttacatta ttttataatt ataaacaagt
    18061 ttgattttaa aatcatatta attattaaca agagatttat ttttctaacc ccaaaagtta
    18121 tgcttggtca aagagtaact tataatatcc atacccctca tagggcttac agtttaaatt
    18181 gcgggtataa aatacatcta ttttcaaata aaataaagca tactccaatt acaacgagtg
    18241 actaaaataa aaatatcatc caagtcaatc actgtttgct ctgcaaaata tgtcagcctt
    18301 ctcaaactta ccagcactta aatatttaag cacacaatca ttccttgagg gagttcactt
    18361 atttcaagag aaacatttcg tcttcccaca caactaatcc gcccaatata gctaaatttg
    18421 tactcagtaa tatcatttgt tatgcccccc ccaaaatatt caagctcctt cacagataaa
    18481 aaacgacaat ttacaaagcc tcattagtta taactatgga aatacctatt ttagtatatc
    18541 ctataaagtt agaattactt cttcttgcac ctaattccat agttgaaaaa caagaataat
    18601 tttctcctac agctggatcc atatagcttc catcaggtct acacaacacc cagtggaggc
    18661 cgacaggaac tccaataaag gatacagcaa cagcttccaa aactctttgg ttagaagata
    18721 acacatctcg ttgatgtact atattacatc ccatgccaat taataagtcc cttgcatcgg
    18781 gataaataaa actaattact tgcgaaaaaa tgttactttc ttcaacgaca taagcattca
    18841 atccaagaag cctcgtagcc atgactatct tatcaggcat agaataaccg gcatccgcga
    18901 tattatcttt gtgtattctc ggattataat taccactagt tattaagtat aaatcccgct
    18961 cacaacgatt atccaaatca agagaactct tccttgtcat ttcggacata gaccctttgt
    19021 attgtggtat tttatcaacc cctaattcct ttgcagcaca gagtaaactg gcagctccac
    19081 aagaattgtc atacatctgc ttcctcgcca aaaaacaacc ttgttcgttt actctattct
    19141 catttaccct cacatcagtc tgatgagata tcatatattc tcttggtata gccccctgca
    19201 ataaaacccc atataacata acacctgtat gaatacatcc ccgtttgcaa ggcttccgtt
    19261 gttccgacat cgacgagtaa ttgcagcaca gtattgcatc cagcaatgcg aactactatg
    19321 agaattagct gaccgggtcc ttattccccg aacgaacttt aaatccaaaa cctcatactc
    19381 ttttccgtcg tcatcacgtc cgtcaccata acaacctttc agcatccacc cttttattaa
    19441 gagttaatgt ccatcagcat cagcaattaa taccgcctga tactgacata tttttgtcgt
    19501 atttacgtcc ataatcggtt attgtgatac aggtcacacc agcttatttg ctattgttac
    19561 aataaccacc ttggttgaga gctgtttttt caactttgtt atcaactccc ccaactcctt
    19621 ggtcagtaag gctgccgacc gagcacagtg aatagatagt atccgcaggt accaccatgc
    19681 ttacttaata ctcagaaggc gaagtgctct ccaattaact ctgccgcact atgcttataa
    19741 gctgcttttc aatgccgtct atcaacatgt ccagttcatt caatctgttg tactggtaat
    19801 ccaacgcagt aatgatatat gcaggtattc tttcatccac tccagtaacg tttcatgcaa
    19861 agcattaatt tacgcgctac ggaacttcac cagttgactg agcaaacgat gtgggaccag
    19921 tactgcctgc agttcttcta ttgttacagc gatttcttta ccaaacagct gaaccaccat
    19981 caagataaag atagcctgaa cattcatcgc accattttgt taccaatggc aaaagattta
    20041 acgaaccgtg cctgcatcag gcggactttt tatccctgct ttgttagctc cctaaccttg
    20101 tacagagagc ctccataagc tcccatatca agcagacatg gttcgcagtt actgacatac
    20161 accagaaaac tctgacagca gactcactta tcctccactt cactcatatc cttattggtg
    20221 agatgaacct gaatgacatg ttttgcgaca taaacgccaa ccagtgtata tttcacctgt
    20281 agagattcta caatctgtag cgttatgcat ctaatgttag gctctatgat gtcagaaatc
    20341 tgcgagactc tacaccttct gcatttacct ttatttatca ccatgctcct cagttaacgg
    20401 agaacgtgtt cattgcattt tgtcacttac atttcgcctt tggtatcggg gggatatagg
    20461 ttccaacgtt ccgcatgaga acctgaaccg gcttcccgcc accttgatac cagatgccgc
    20521 acaggtagtc aacaagtgtc acgaacggtg caatagtgat ccacacccaa cgcctgaaat
    20581 cagatccagg gggtaatctg ctctcctgat tcaggagagc ttatggtcac ttttgagaca
    20641 gttatggaaa ttaaaatcct gcacaagcag ggaatgagta gccgg
//
LOCUS       DAJTNP010000070        20602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTNP010000070 DAJTNP010000000
VERSION     DAJTNP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            86..385
                     /locus_tag="OO160_002737"
     CDS             86..385
                     /locus_tag="OO160_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5524794.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            395..919
                     /gene="ygaP"
                     /locus_tag="OO160_002738"
     CDS             395..919
                     /gene="ygaP"
                     /locus_tag="OO160_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5524795.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO160_002739"
     CDS             complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO160_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5524796.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2038..2487
                     /gene="alaE"
                     /locus_tag="OO160_002740"
     CDS             2038..2487
                     /gene="alaE"
                     /locus_tag="OO160_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5524797.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2524..2868)
                     /locus_tag="OO160_002741"
     CDS             complement(2524..2868)
                     /locus_tag="OO160_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5524798.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3020..3250
                     /locus_tag="OO160_002742"
     CDS             3020..3250
                     /locus_tag="OO160_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524799.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3597..3842
                     /gene="nrdH"
                     /locus_tag="OO160_002743"
     CDS             3597..3842
                     /gene="nrdH"
                     /locus_tag="OO160_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5524800.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3839..4249
                     /gene="nrdI"
                     /locus_tag="OO160_002744"
     CDS             3839..4249
                     /gene="nrdI"
                     /locus_tag="OO160_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5524801.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4222..6366
                     /gene="nrdE"
                     /locus_tag="OO160_002745"
     CDS             4222..6366
                     /gene="nrdE"
                     /locus_tag="OO160_002745"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5524802.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6376..7335
                     /gene="nrdF"
                     /locus_tag="OO160_002746"
     CDS             6376..7335
                     /gene="nrdF"
                     /locus_tag="OO160_002746"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5524803.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7690..8892
                     /gene="proV"
                     /locus_tag="OO160_002747"
     CDS             7690..8892
                     /gene="proV"
                     /locus_tag="OO160_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5524804.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8885..9949
                     /gene="proW"
                     /locus_tag="OO160_002748"
     CDS             8885..9949
                     /gene="proW"
                     /locus_tag="OO160_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5524805.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10007..10999
                     /gene="proX"
                     /locus_tag="OO160_002749"
     CDS             10007..10999
                     /gene="proX"
                     /locus_tag="OO160_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5524806.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11191..12370
                     /locus_tag="OO160_002750"
                     /pseudo
     CDS             11191..12370
                     /locus_tag="OO160_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO160_002751"
     CDS             12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO160_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5524807.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13221..13556
                     /gene="ygaH"
                     /locus_tag="OO160_002752"
     CDS             13221..13556
                     /gene="ygaH"
                     /locus_tag="OO160_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5524808.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13647..14177
                     /gene="emrR"
                     /locus_tag="OO160_002753"
     CDS             13647..14177
                     /gene="emrR"
                     /locus_tag="OO160_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5524809.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14304..15476
                     /gene="emrA"
                     /locus_tag="OO160_002754"
                     /pseudo
     CDS             14304..15476
                     /gene="emrA"
                     /locus_tag="OO160_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15493..17031
                     /gene="emrB"
                     /locus_tag="OO160_002755"
     CDS             15493..17031
                     /gene="emrB"
                     /locus_tag="OO160_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5524810.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO160_002756"
     CDS             complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO160_002756"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5524811.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO160_002757"
     CDS             complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO160_002757"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5524812.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19389..19817)
                     /locus_tag="OO160_002758"
     CDS             complement(19389..19817)
                     /locus_tag="OO160_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5524813.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO160_002759"
     CDS             complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO160_002759"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5524814.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4971 a         5270 c         5147 g         5214 t
ORIGIN      
        1 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact
       61 taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtaccga
      121 acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat
      181 gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagcgc
      241 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca acagccaacg
      301 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
      361 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
      421 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
      481 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
      541 aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
      601 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
      661 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
      721 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taaacggcgt
      781 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
      841 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
      901 gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
      961 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     1021 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc agtgaattta
     1081 tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc agagctatta
     1141 cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt
     1201 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     1261 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     1321 atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
     1381 accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat
     1441 atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc tgttattttc
     1501 tgaaactctc agaataattt caagattata tattagccat tttgatgagt aatttaggtt
     1561 attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca aaaccgaagt
     1621 gaataaaaat acaatataac agaataaaag aaaacacccc tcactggtgc tttctgctgt
     1681 taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac aaacaccgaa
     1741 ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat taaaggcaag
     1801 atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa tccacaatgt
     1861 acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat aaaaattttt
     1921 cactaattga ttagtcatag gcagcgatat acgctatgca aaaatgcaga tggcaatgag
     1981 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
     2041 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
     2101 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
     2161 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
     2221 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
     2281 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
     2341 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
     2401 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
     2461 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
     2521 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
     2581 aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
     2641 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
     2701 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
     2761 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
     2821 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
     2881 ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     2941 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     3001 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     3061 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc
     3121 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct
     3181 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc
     3241 cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     3301 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     3361 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     3421 tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat cactatttgc
     3481 tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     3541 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     3601 gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     3661 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag
     3721 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     3781 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     3841 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     3901 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     3961 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     4021 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     4081 tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     4141 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     4201 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     4261 atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag
     4321 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     4381 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     4441 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     4501 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     4561 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     4621 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     4681 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     4741 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     4801 gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     4861 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     4921 gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg
     4981 gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     5041 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg
     5101 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac
     5161 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     5221 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     5281 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac
     5341 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     5401 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt
     5461 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc
     5521 acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     5581 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     5641 ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc
     5701 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg
     5761 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     5821 ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     5881 gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     5941 atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct
     6001 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     6061 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg
     6121 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     6181 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     6241 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     6301 atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     6361 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     6421 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     6481 gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg
     6541 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     6601 ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc
     6661 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     6721 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     6781 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     6841 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     6901 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     6961 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     7021 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     7081 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     7141 tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     7201 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     7261 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     7321 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     7381 tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     7441 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     7501 atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc
     7561 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     7621 gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat aaagaaatca
     7681 ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt tggtgagcat
     7741 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     7801 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     7861 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     7921 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac
     7981 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     8041 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     8101 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     8161 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     8221 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     8281 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     8341 attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt
     8401 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     8461 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa
     8521 gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt
     8581 tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     8641 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     8701 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     8761 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     8821 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     8881 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     8941 atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg
     9001 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     9061 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     9121 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     9181 attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcacctggca
     9241 gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat
     9301 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg
     9361 tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat
     9421 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     9481 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct
     9541 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     9601 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     9661 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     9721 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     9781 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     9841 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     9901 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     9961 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
    10021 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
    10081 gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac
    10141 gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt
    10201 ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
    10261 ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
    10321 tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga
    10381 ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
    10441 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
    10501 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
    10561 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
    10621 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
    10681 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
    10741 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
    10801 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
    10861 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
    10921 ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
    10981 gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg
    11041 ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg
    11101 cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc
    11161 agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg
    11221 gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc
    11281 cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt
    11341 attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt
    11401 gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg
    11461 ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt
    11521 ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat
    11581 aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca
    11641 cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct
    11701 tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca
    11761 gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat
    11821 aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc
    11881 tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt
    11941 ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt
    12001 gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac tttcatggct
    12061 ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc tggtgctgga
    12121 cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa tacatcctga
    12181 tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg
    12241 ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg
    12301 cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc
    12361 cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag
    12421 cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt
    12481 aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat
    12541 ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt
    12601 cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat
    12661 catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt
    12721 gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc
    12781 actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg
    12841 cctgacggat gaggtttttg ccgccgccac cgcaaagctg gtacgcaata atcgccgctg
    12901 gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac
    12961 ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc
    13021 attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa
    13081 acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc
    13141 tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc
    13201 attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg
    13261 cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa
    13321 ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc
    13381 tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc
    13441 tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa
    13501 cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca
    13561 caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg
    13621 gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc
    13681 taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc
    13741 tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg
    13801 ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta
    13861 ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg
    13921 ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct
    13981 gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc
    14041 agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg
    14101 agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc
    14161 tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat
    14221 gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag
    14281 ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac
    14341 cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa
    14401 ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg
    14461 atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga
    14521 cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg
    14581 acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc
    14641 gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa
    14701 aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca
    14761 acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc
    14821 aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac
    14881 tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc
    14941 tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac
    15001 cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt
    15061 gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca
    15121 ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg
    15181 atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga
    15241 tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat
    15301 acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc
    15361 aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca
    15421 gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca
    15481 gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg
    15541 attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg
    15601 atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact
    15661 tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc
    15721 ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt
    15781 ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc
    15841 gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc
    15901 tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc
    15961 ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg
    16021 attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc
    16081 gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg
    16141 cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt
    16201 cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat
    16261 aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt
    16321 atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag
    16381 gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt
    16441 ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg
    16501 gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa
    16561 ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc
    16621 tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg
    16681 gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata
    16741 accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta
    16801 aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg
    16861 caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc
    16921 gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg
    16981 tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta
    17041 agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat
    17101 gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac
    17161 ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta
    17221 agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac
    17281 gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag
    17341 actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt
    17401 accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct
    17461 ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc
    17521 gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg
    17581 atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat
    17641 tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca
    17701 tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt
    17761 caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc
    17821 tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc
    17881 ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata
    17941 ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag
    18001 caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc
    18061 gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac
    18121 tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc
    18181 cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc
    18241 agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc
    18301 ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac
    18361 agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac
    18421 agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca
    18481 ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg
    18541 tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg
    18601 agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc
    18661 tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca
    18721 ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag
    18781 cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag
    18841 aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg
    18901 tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg
    18961 tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac
    19021 atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc
    19081 atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg
    19141 gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc
    19201 agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt
    19261 aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg
    19321 acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag
    19381 gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag
    19441 cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat
    19501 cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact
    19561 gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat
    19621 ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct
    19681 gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact
    19741 tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc
    19801 gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg
    19861 cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac
    19921 gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag
    19981 cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg
    20041 cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt
    20101 caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt
    20161 cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa
    20221 tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt
    20281 agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc
    20341 catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa
    20401 aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa
    20461 attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg
    20521 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
    20581 tctatccagc tgagctacgg at
//
LOCUS       DAJTNP010000071        20354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTNP010000071 DAJTNP010000000
VERSION     DAJTNP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            82..351
                     /locus_tag="OO160_002760"
     CDS             82..351
                     /locus_tag="OO160_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5524815.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO160_002761"
     CDS             345..710
                     /locus_tag="OO160_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5524816.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO160_002762"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO160_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5524817.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO160_002763"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO160_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5524818.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO160_002764"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO160_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5524819.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO160_002765"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO160_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5524820.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO160_002766"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO160_002766"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5524821.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TTTKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO160_002767"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO160_002767"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5524822.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO160_002768"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO160_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO160_002769"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO160_002769"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5524823.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO160_002770"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO160_002770"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5524824.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO160_002771"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO160_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5524825.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO160_002772"
     CDS             complement(16430..17188)
                     /locus_tag="OO160_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5524826.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO160_002773"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO160_002773"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5524827.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO160_002774"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO160_002774"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5524828.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="OO160_002775"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="OO160_002775"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5524829.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         4792 a         5606 c         5144 g         4812 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcatggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caagtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggtggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc acttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctag
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaa
//
LOCUS       DAJTNP010000072        20288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTNP010000072 DAJTNP010000000
VERSION     DAJTNP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO160_002776"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO160_002776"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5524830.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO160_002777"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO160_002777"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5524831.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO160_002778"
     CDS             complement(1728..2999)
                     /locus_tag="OO160_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524832.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="OO160_002779"
     CDS             complement(3182..4387)
                     /locus_tag="OO160_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5524833.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="OO160_002780"
     CDS             complement(4487..4843)
                     /locus_tag="OO160_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5524834.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OO160_002781"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO160_002781"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO160_002782"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO160_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5524835.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OO160_002783"
     CDS             6773..9829
                     /locus_tag="OO160_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5524836.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OO160_002784"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OO160_002784"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5524837.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OO160_002785"
     CDS             10336..10524
                     /locus_tag="OO160_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5524838.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OO160_002786"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OO160_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5524839.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OO160_002787"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OO160_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5524840.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OO160_002788"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OO160_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5524841.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OO160_002789"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OO160_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5524842.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OO160_002790"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OO160_002790"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5524843.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OO160_002791"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OO160_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5524844.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OO160_002792"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OO160_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5524845.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO160_002793"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO160_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5524846.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO160_002794"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO160_002794"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5524847.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4861 c         5177 g         4934 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgacaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaacta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gtttgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    20281 atgctgat
//
LOCUS       DAJTNP010000073        20238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTNP010000073 DAJTNP010000000
VERSION     DAJTNP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..280
                     /gene="raiA"
                     /locus_tag="OO160_002795"
     CDS             <1..280
                     /gene="raiA"
                     /locus_tag="OO160_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5524848.1"
                     /translation="DRLAKLEKWQTHLINPHIILSKEPQGFVADATINTPNGVLVASG
                     KHEDMYTAINELINKLERQLNKLQHKGEARRAATSVKDANFVEEVEEE"
     misc_feature    383..508
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            384..431
                     /gene="pheL"
                     /locus_tag="OO160_002796"
     CDS             384..431
                     /gene="pheL"
                     /locus_tag="OO160_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5524849.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            530..1690
                     /gene="pheA"
                     /locus_tag="OO160_002797"
     CDS             530..1690
                     /gene="pheA"
                     /locus_tag="OO160_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5524850.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1733..2854)
                     /gene="tyrA"
                     /locus_tag="OO160_002798"
     CDS             complement(1733..2854)
                     /gene="tyrA"
                     /locus_tag="OO160_002798"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5524851.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2865..3935)
                     /gene="aroF"
                     /locus_tag="OO160_002799"
     CDS             complement(2865..3935)
                     /gene="aroF"
                     /locus_tag="OO160_002799"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5524852.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4145..4510
                     /locus_tag="OO160_002800"
     CDS             4145..4510
                     /locus_tag="OO160_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5524853.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4660..5178
                     /locus_tag="OO160_002801"
     CDS             4660..5178
                     /locus_tag="OO160_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5524854.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5168..6393
                     /gene="dgcN"
                     /locus_tag="OO160_002802"
                     /pseudo
     CDS             5168..6393
                     /gene="dgcN"
                     /locus_tag="OO160_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6409..6891
                     /locus_tag="OO160_002803"
     CDS             6409..6891
                     /locus_tag="OO160_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5524855.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(6968..7315)
                     /gene="rplS"
                     /locus_tag="OO160_002804"
     CDS             complement(6968..7315)
                     /gene="rplS"
                     /locus_tag="OO160_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5524856.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7357..8124)
                     /gene="trmD"
                     /locus_tag="OO160_002805"
     CDS             complement(7357..8124)
                     /gene="trmD"
                     /locus_tag="OO160_002805"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5524857.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8155..8703)
                     /gene="rimM"
                     /locus_tag="OO160_002806"
     CDS             complement(8155..8703)
                     /gene="rimM"
                     /locus_tag="OO160_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5524858.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8722..8970)
                     /gene="rpsP"
                     /locus_tag="OO160_002807"
     CDS             complement(8722..8970)
                     /gene="rpsP"
                     /locus_tag="OO160_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5524859.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9107..10468)
                     /gene="ffh"
                     /locus_tag="OO160_002808"
     CDS             complement(9107..10468)
                     /gene="ffh"
                     /locus_tag="OO160_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5524860.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10635..11426
                     /locus_tag="OO160_002809"
     CDS             10635..11426
                     /locus_tag="OO160_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5524861.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11447..12733
                     /locus_tag="OO160_002810"
     CDS             11447..12733
                     /locus_tag="OO160_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5524862.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12788..13381)
                     /gene="grpE"
                     /locus_tag="OO160_002811"
     CDS             complement(12788..13381)
                     /gene="grpE"
                     /locus_tag="OO160_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5524863.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13504..14382
                     /gene="nadK"
                     /locus_tag="OO160_002812"
     CDS             13504..14382
                     /gene="nadK"
                     /locus_tag="OO160_002812"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5524864.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14468..16129
                     /gene="recN"
                     /locus_tag="OO160_002813"
     CDS             14468..16129
                     /gene="recN"
                     /locus_tag="OO160_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5524865.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16278..16619
                     /gene="bamE"
                     /locus_tag="OO160_002814"
     CDS             16278..16619
                     /gene="bamE"
                     /locus_tag="OO160_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5524866.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16681..16971)
                     /locus_tag="OO160_002815"
     CDS             complement(16681..16971)
                     /locus_tag="OO160_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5524867.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(16961..17398)
                     /gene="ratA"
                     /locus_tag="OO160_002816"
     CDS             complement(16961..17398)
                     /gene="ratA"
                     /locus_tag="OO160_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5524868.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17569..18051
                     /gene="smpB"
                     /locus_tag="OO160_002817"
     CDS             17569..18051
                     /gene="smpB"
                     /locus_tag="OO160_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5524869.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18266..18628
                     /gene="ssrA"
                     /locus_tag="OO160_002818"
     tmRNA           18266..18628
                     /gene="ssrA"
                     /locus_tag="OO160_002818"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18833..20074
                     /locus_tag="OO160_002819"
     CDS             18833..20074
                     /locus_tag="OO160_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5524870.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5202 a         5299 c         4766 g         4971 t
ORIGIN      
        1 agaccgtctc gccaaactgg aaaaatggca aacacatctg attaatccac atatcattct
       61 gtccaaagag ccacaagggt ttgttgctga cgccacaatc aatacaccta acggcgttct
      121 ggttgccagt ggtaaacatg aagatatgta caccgcaatt aacgaattga tcaacaagct
      181 ggaacggcag ctcaataaac tgcagcacaa aggcgaagca cgtcgtgccg caacatcggt
      241 gaaagacgcc aacttcgtcg aagaagttga agaagagtag tcctttatat tgagtgtatc
      301 gccaacgcgc cttcgggcgc gttttttgtt gacagcgtga aaacagtacg ggtactgtac
      361 taaagtcact taaggaaaca aacatgaaac acataccgtt tttcttcgca ttctttttta
      421 ccttcccctg aatgggaggc gtttcgtcgt gtgaaacaga atgcgaagac gaacaataag
      481 gcctcccaaa tcggggggcc ttttttattg ataacaaaaa ggcaacacta tgacatcgga
      541 aaacccgtta ctggcgctgc gagagaaaat cagcgcgctg gatgaaaaat tattagcgtt
      601 actggcagaa cggcgcgaac tggccgtcga ggtgggaaaa gccaaactgc tctcgcatcg
      661 cccggtacgt gatattgatc gtgaacgcga tttgctggaa agattaatta cgctcggtaa
      721 agcgcaccat ctggacgccc attacattac tcgcctgttc cagctcatca ttgaagattc
      781 cgtattaact cagcaggctt tgctccaaca acatctcaat aaaattaatc cgcactcagc
      841 acgcatcgct tttctcggcc ccaaaggttc ttattcccat cttgcggcgc gccagtatgc
      901 tgcccgtcac tttgagcaat tcattgaaag tggctgcgcc aaatttgccg atatttttaa
      961 tcaggtggaa accggccagg ccgactatgc cgtcgtaccg attgaaaata ccagctccgg
     1021 tgccataaac gacgtttacg atctgctgca acataccagc ttgtcgattg ttggcgagat
     1081 gacgttaact atcgaccatt gtttgttggt ctccggcact actgatttat ccaccatcaa
     1141 tacggtctac agccatccgc agccattcca gcaatgcagc aaattcctta atcgttatcc
     1201 gcactggaag attgaatata ccgaaagtac gtctgcggca atggaaaagg ttgcacaggc
     1261 aaaatcaccg catgttgctg cgttgggaag cgaagctggc ggcactttgt acggtttgca
     1321 ggtactggag cgtattgaag caaatcagcg acaaaacttc acccgatttg tggtgttggc
     1381 gcgtaaagcc attaacgtgt ctgatcaggt tccggcgaaa accacgttgt taatggcgac
     1441 cgggcaacaa gccggtgcgt tggttgaagc gttgctggta ctgcgcaacc acaatctgat
     1501 tatgacccgt ctggaatcac gcccgattca cggtaatcca tgggaagaga tgttctatct
     1561 ggatattcag gctaatcttg aatcagcgga aatgcaaaaa gcattgaaag agttagggga
     1621 aatcacccgt tcaatgaagg tattgggctg ttacccaagt gagaacgtag tgcctgttga
     1681 tccaacctga tgaaaaggtg ccggatgatg tgaatcatcc ggcactggat tattactggc
     1741 gattgtcatt cgcctgacgc aataacacgc ggctttcact ctgaaaacgc tgtgcgtaat
     1801 cgccgaacca gtgctccacc ttgcggaaac tgtcaataaa cgcctgctta tcgccctgct
     1861 ccagcaactc aatcgcctcg ccgaaacgct tatagtaacg tttgattaac gccagattac
     1921 gctctgacga cataatgatg tcggcataaa gctgcggatc ctgagcaaac agtcgcccga
     1981 ccatcgccag ctcaaggcgg taaatcggcg aagagagcgc cagaagttgc tcaagctgaa
     2041 cattttcttc tgccaggtgc agcccgtaag caaaagtagc aaagtggcgc agagcctgaa
     2101 taaacgccat attctgatcg tgctcgacag cgctaatacg atgcaaccga gcgccccaga
     2161 cctgaatttg ctccagaaac cattggtatg cttccggctt acgtccatca caccagacca
     2221 caacttgctt tgccaggcta ccgctgtccg ggccgaacat cgggtgtaac cccagtaccg
     2281 ggccatcgtg cgccgccagc atggcctgta atggtccatt tttcactgat gccagatcaa
     2341 ccagaataca atctttcggt aaaggcggta atttgccgat aacttgctca gtaacgtgga
     2401 ttggcacact aacaatcacc attccggcat cggcaacaat atcagccgct cgatcccagt
     2461 catgttgctc cagaatccgc acctgataac ccgatagtgt cagcatcttc tcgaacaggc
     2521 gtcccatctg accgccaccg ccgacgataa ccaccggacg cagtgacgga caaagtgttt
     2581 taaatccttt gtcgttttca ctggagtaag attcacgcat cacccgacgc aaaacatcct
     2641 caatcagatc tggcggtaca cccagagctt ccgcctctgc acgacgcgag gccaacatag
     2701 atgcctcgcg ctccggaaca taaataggca gtccaaagcg gcttttcacc ttgcccactt
     2761 cagcaaccag ttccagacgc ttcgctaata aattcagcag cgctttatcg acttcatcaa
     2821 tttgatcgcg taatgcggtc aattcagcaa ccataataaa cctcttaagc cacgcgagcc
     2881 gtcagctgcc cgttcagatc ctgatgaatt tcacgcagca aggcatcggt catttcccag
     2941 ctaatgcagg catcggttac ggatacaccg tatttcattt cactgcgcgg ttgctcagaa
     3001 gactgattgc cctcgtggat attactttcg atcatcagac caataattga gcgattacca
     3061 tctttgattt gagcaaccac ggattctgcc accgcaggct gacggcgata atctttattg
     3121 gaattaccgt ggctgcaatc taccatcaga gacgggcgca gtcccgcctg ttccatctct
     3181 ttttcacatt gcgcaacatc cgcagggcta tagttcggcg ctttaccacc gcgcaggatc
     3241 acatgcccgt ccggattccc ctgagtttgt agcaacgcaa cctgccctgc ctggttaatg
     3301 ccaacaaaac agtgcggctg ggcggcggcg cgcatagcgt taattgctgt tgccagactg
     3361 ccgtcggtgc cgtttttaaa accaaccggc atggaaagcc cggaggccat ttcacggtga
     3421 gtttgcgatt ccgttgtacg agcaccaatt gctgaccagc taaacagatc gcccaggtat
     3481 tgcgggctat tcggatctaa cgcttccgtc gccagtggca gtcccatatt caccagctca
     3541 agcaacaatt tacgcgcgat ttgcagcccg gcttctacat caaaagagcc atccatatgg
     3601 ggatcgttaa ttaacccttt ccagccgaca gtggtacggg gtttttcaaa atagacgcgc
     3661 attaccagat agaggctatc gctgacctct gcggcaagag ctttaaatcg acgagcatat
     3721 tccagagcag tttccggatc atgaatggaa caaggaccac atactaccag cagacgagga
     3781 tcgcgcccgg cgataatatc tgaaatggtt ttacgcgagt cagcaatctg ggcttcttgt
     3841 tgcaggctca atggaaaagc ggccttcagt tgttccggag tcattaaaac ctgttcgtcg
     3901 gtaatatgta cgttattcag cgcgtcttta tgcatgatgg cgatcctgtt tatgctcgtt
     3961 tgcgatagtt gctcctcagc gaggatgacg taacgataac acataaagta aagttttcaa
     4021 tccatatttc gtacattttt atttacacag gcaatttagt cgcgctttca acccttgctt
     4081 ctgtatagat aaatttacat cccctttaaa aacaatccgc tatgctttga aaaaggagaa
     4141 agaaatgatg aaaaagttta tcgccccctt gttggcttta ctggttagcg gatgtcagat
     4201 tgatccttat actcacgcgc caaccttgac cagcaccgac tggtatgatg tcggtatgga
     4261 agatgcgata tcgggcagcg ccataaaaga tgacgatgca tttagcgatt cacaggcgga
     4321 tcgcggtcta taccttaaag gatatgccga agggcaaaag aaaacttgcc agaccgattt
     4381 tacttatgcc cggggacttt ccggtaaaag ctttcctgcc agctgcaaca atgttgaaaa
     4441 tgccagccaa ctgcatgaag tatggcaaaa aagggctgat gaaaatgcca gcacaatacg
     4501 attaaattaa ttaaaacccc acaaataaat taaacataag attttactca ttgtgtttta
     4561 tttctcacat tgatgacggt cgcatgaaaa aatgatattt taatgagcat cctgaaaaaa
     4621 tatcgatgca tttcgagcga agatggtggg gatccctgaa tgcgtttttc tcaccgactg
     4681 ttcttacttc tgattctcct gctgacggga gcaccaatac tcgctcaaga gccttctgac
     4741 gttgcgaaaa acgtgcgtat gatggtttcc ggtattgtca gttacacccg ctggcctgcg
     4801 ctatcagggc caccaaagtt atgtatcttt tcatcttcac gttttagtac cgccctacaa
     4861 gagaatgccg cgacatcttt gccttatctg cctgtcatca ttcacactca acaagaggcg
     4921 atgatttcag gctgcaatgg tttttatttt ggcaacgagt caccgacatt ccagatggaa
     4981 ttaaccgaac aatatccgtc aaaggcgtta ttattaattg ccgaacaaaa taccgagtgc
     5041 attattggta gcgccttttg tctgatcatc cacaataatg acgtcagatt tgccgtaaac
     5101 ctggatgcct catcgcgtag cggtgtaaag gtcaacccgg atgtcttaat gctcgcacgg
     5161 aagaaaaatg atggataacg ataattctct taataagcgc cccacgttta aaagagcatt
     5221 acgcaacatc agtatgacca gcatatttat cactatgatg ctgatctggt tgctgctttc
     5281 cgtgacctcg gtgctgaccc tgaaacagta cgcgcaaaaa aacctggcac tgacagcagc
     5341 aacaatgact tacagtctgg aagcagctgt cgtttttgcc gatggccctg cagcaactga
     5401 aacactggca gcgctgggcc agcaagggca ttttcaactg cagaagtacg tgataagcag
     5461 caaaatattc tggcatcctg gcattacccc cgtaaggatc caggcgatac tttcagcaat
     5521 ttcataagcc actggctctt ccctgccccc atcattcagc cgattcgtca caatggtgaa
     5581 accattggcg aagtacgctt aaccgctcgc gacagttcaa tcagccattt tatctggttt
     5641 tcgctcgccg tactgaccgg ttgtattctg ctggcatcag gcatcgcaat taccctcacc
     5701 cgccatttgc acaatggcct ggtggaagca ctgaaaaata tcaccgatgt cgtacatgat
     5761 gtgcgttcca accgcaattt ttcccgacga gtttcggaag aacgtatcgc tgagtttcac
     5821 cgcttcgctc tcgacttcaa cagtctgctg gatgaaatgg aagagtggca gcttcgttta
     5881 caggccaaaa atgcgcagct tctgcgtacc gcgctacatg acccattaac cgggctggct
     5941 aaccgcgcag cgtttcgtag cggcatcaat acgttgatga acaattccga tgcccgaaaa
     6001 acgtcggcgt tactatttct tgatggcgat aatttcaaat atatcaatga tacctggggt
     6061 catgcgacgg gcgatagggt cttgattgaa atcgcaaaac ggttagctga atttggcggg
     6121 ctgcgacata aagcataccg cctgggcggc gatgaattcg ctatggtgct ctatgatgta
     6181 cagtcggaat ctgaagtgca gcagatatgc tcagcactga cacaaatctt taatctcccg
     6241 tttgatcttc ataatggcca tcagaccacc atgacattaa gcattggtta cgcgatgacc
     6301 attgagcacg cctctgcgga aaaattacaa gagcttgccg atcacaatat gtatcaggcc
     6361 aaacaccagc gtgccgaaaa gctggtgaga taacaaggat atatcgatat gataaagcac
     6421 ctggtagcac ccctgatttt cacctcacta atactgactg gctgccagtc ccctcaggga
     6481 aagtttactc ctgagcaagt cgccgctatg caatcttatg gatttactga atccgccggc
     6541 gactggtcgc tgggcttatc agatgccatt ctgttcgcaa aaaatgacta caaattgctc
     6601 ccggaaagcc agcaacagat ccaaaccatg gcagccaaat tggcctcgac agggctaaca
     6661 catgcccgta tggatggaca caccgataac tatggtgaag acagttacaa cgaaggctta
     6721 tcattgaaac gggcgaatgt cgtggccgat gcatgggcta ttggtggaca aattccacgc
     6781 agcaatctca ccacacaggg tttaggaaaa aaatatccca tagccagtaa caagaccgcc
     6841 cagggccgcg ccgagaaccg ccgcgtcgca gtggtgattg caacccctta aaacacgagc
     6901 aaacagacaa aaaaagggcc agccaattgg ccagcccttc ttaacaggat gtcgcttaag
     6961 cgaaatctta gttaagacgc tctttgatac gagcagcctt accagtacgc tcacgcaggt
     7021 agtacagttt agctttacga acagcaccac gacgtttgac agaaatgctg tcaactaccg
     7081 gagagtgagt ctggaagaca cgctcaacgc cttcgccgtt ggaaatttta cgaacagtga
     7141 atgcagagtg cagaccgcgg ttacgaatag cgataaccac gccctcgaat gcctgcagac
     7201 gttttttgga accttcaaca acccatactt tcacttccac ggtatcaccc ggacggaagg
     7261 aaggtacgtc ctgcttcatc tgctcttgtt caagttgctt aataatgttg ctcataattt
     7321 aatctcttat cctgggtaaa ctgatctctc gggggcttac gccatcccat catgtttatg
     7381 ttgctgttgt gcgtgttccg ttttgaactc cgccagcaac cttgcttgct cttcagtcag
     7441 agccaggttt tccagaagtt caggtcttct aagccaggta cggcccagcg actgtttcaa
     7501 acgccagcga cgtatctcgg catggttgcc cgacagtaac actggcggaa cttccatccc
     7561 ttctaacacc tcaggccgcg tatagtgcgg gcaatccagc aatccttcag caaaggaatc
     7621 ttccgttgcc gaggcttcat gtcccagtac tcccggaata aaccgggaaa cggagtcaat
     7681 cagcgtcatt gctggtaact caccaccact gagaacgtaa tcgccgattg accattcttc
     7741 gtcaatttcg gtttggatca cgcgctcatc tataccttcg tagcgaccgc acaccagaat
     7801 caatttttga ttcgttgcca gttcgctgac gcccgcttga tcaagcttgc gtccctgtgg
     7861 tgacagataa atcacctttg cgccttcacc cgccgcggct tttgctgcat gaatggcgtc
     7921 ccgcaagggt tgcaccatca ttaacatccc cggtccgccg ccgtaaggac gatcgtccac
     7981 ggtacggtgc cggtcatgcg tgaagtcgcg aggactccaa ctctggatgc tcagcaggcc
     8041 atttttaact gcccggccag ttaccccgta atcggtaatt gcgcggaaca tttcaggaaa
     8101 caggctaatt atgccaatcc acatagcgcc gccttttacc gtttatccgg tggtttaaaa
     8161 accaggatcc caatctactt cgattgaacg agtagtgaga tcgactttct tgataacctg
     8221 cccatcgagg aacggtacga gacgttcctt gataccaaac gcatctttca ggtttgcctt
     8281 aatgacgaga acgtcattag atccggtttc catcatatca acgactttac cgagatcgta
     8341 gccttcagtg gttactacct ggcagcccat caggtctttc cagtagtagt ccccctcttc
     8401 aagctgaggc agctgcgatg aatccacgac aatttcacaa ttcgtcagca ggttcgccgc
     8461 atcacgatcg tcaacgcctt tcagcttgat gatcatgtcc tgattgtggt gcttccagct
     8521 ttccagctgg acttgctgcc actgacccgc cttctggata aaccagggct gatagtcaaa
     8581 aatgctttcg gcgtcttcgg tggaagaaaa cactctgagc cacccacgaa taccgtaaga
     8641 cgaacccatt tttcccaaaa cgatgggatc aacaggtgct tgcgcggtga gttgtttgct
     8701 catcatgacc accgtgacag attaagctgc tttgtttact tctttgatca gcgcagcaac
     8761 gcgatcagaa atagttgcgc cctggccaac ccagtgagcg atgcgatcca gatccaggcg
     8821 agtgccttct tctttttcgc tagcgattgg gttgaagaaa ccaacgcgct cgatgaagcg
     8881 accgttgcgt gcattacggc tgtcagcgac aacaacctgg tagaacggac gctttttagc
     8941 gccgtgacgt gctaaacgaa tagttaccat aacatcctct tgtgtgaata aaacaaccgg
     9001 accccatcga ggaacggagt ccggtgtcat attaaaagcc cgaaaatttt actgatttct
     9061 ggagaaaatg caatcagcag ttaataactc tgtcgcataa ggtcatttag cgaccaggaa
     9121 atcccggcgg catcattccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
     9181 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggtta acgtcctgta
     9241 cctgcatccc gcaaccggcg gcgatacgac gcttacgcga acctttgatg atttctggct
     9301 tagcgcgctc tttcatcgtc attgagttga tgatggcttc catacgcacc agcactttat
     9361 cgtccatctg cgacttgacg ttatccggga tctgccccat gcccggcagt ttgcccatca
     9421 agctggccat gccgcccata tttttcatct ggcgcagttg ctcaagaaag tcgttgagat
     9481 cgaagccgtc gccttttttt agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
     9541 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
     9601 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg aggaacttga
     9661 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
     9721 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
     9781 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
     9841 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
     9901 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacaatat
     9961 caaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    10021 tctcaagctg tttgattgcc gccgggcgat agacgtcagc agaaaccacc agcactttct
    10081 tcttgtgctt ctcgcgcagg aaattaccga gtttaccaac gctggttgtt ttaccggcac
    10141 cttgcaggcc tgccatcagc acgaccgcag gcggttgcgc agccagattc agggtctggt
    10201 tctcttcgcc catcgccgca accagttcgt tacggacgat tttgacgaac tcctgccccg
    10261 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    10321 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    10381 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    10441 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    10501 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    10561 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    10621 ctgtcgacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    10681 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    10741 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    10801 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttggtga
    10861 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    10921 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    10981 cccatctgga agcaacgcct gggatgctga tgcacattgg cttatcgctc ttttcctatg
    11041 ccacgctaat tatcgccgcc ctgtacgcgc tgcaactggc gtggattgat taccaactga
    11101 agaacaagaa gctggcattt aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa
    11161 tgttccacat cacgcagatt ggcgtggtgc tgctaacgct cacgctttgc actggcctgt
    11221 tctacatgca caacctattt agcatggaaa atatcgacaa ggctgtgctc tctatcgtgg
    11281 cgtggtttgt ctatattgtg ctgctgtggg gacattacca tgaaggatgg cgtggacgcc
    11341 gcgtcgtctg gtttaacgtt gcgggcgcgg taattctgac actggcctac ttcggcagcc
    11401 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    11461 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    11521 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctt cgacatatgg cgaaacaggg
    11581 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    11641 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgtcgg
    11701 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    11761 actggttttt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    11821 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    11881 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
    11941 tagcggctct ttgagcaaag aagagttgcg cactatcgtg catgaatcgc gctcacaaat
    12001 ttcccgtcgc aatcaggata tgctgctgtc ggtgctcgat ctggaaaaaa tgaccgttga
    12061 tgacatcatg gtaccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    12121 tattctgcgc caactctccc actcacctca cgggcgcatc gtgctttacc gtgattcgct
    12181 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
    12241 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgcctgaagg
    12301 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
    12361 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    12421 tgttggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    12481 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
    12541 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
    12601 agagatccct gtcgcaggca cccgcgtgcg tattggcaag tacgatatcg atattctcga
    12661 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
    12721 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    12781 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    12841 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    12901 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    12961 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    13021 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    13081 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    13141 caatatccag ttcagtacga cgacgcaggt tttccatttc ggcttttacg cgcaaaatac
    13201 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    13261 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    13321 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    13381 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    13441 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    13501 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccctactgca
    13561 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    13621 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    13681 attgggcaac tggctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    13741 gcgcgcacgc tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    13801 ttcctgactg accttgaccc cgataacgcc cagcaacaat tagccgatgt gctggaaggt
    13861 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccaa
    13921 aaacgcatca gcaccgcgat taacgaagtg gtacttcatc ctggcaaagt ggcgcatatg
    13981 atagagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatgggcta
    14041 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcgggcgg tcctattctg
    14101 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    14161 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    14221 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    14281 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaat
    14341 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    14401 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    14461 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    14521 tgattttcat agcggcatga ccgtaataac tggcgagacc ggtgcgggta aatctattgc
    14581 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    14641 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggccctgcg
    14701 ctggctggaa gaaaaccagc ttgaagacgg gcatgaatgt ttgcttcgtc gcgtgatcag
    14761 cagtgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    14821 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcaat tactcaccaa
    14881 acctgagcac caaaaattcc tgcttgatgg ctatgccaat gaaacctctc tacttcagga
    14941 aatgaccgca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    15001 gttaagtcag gaacgcgccg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    15061 cgaatttaat ccgcagcccg gagagtttga gcaaatcgac gaagagtaca aacgtctggc
    15121 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    15181 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    15241 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    15301 tgaagccagc gacgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    15361 gtttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    15421 ccctgaggca ttaccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    15481 tcaggccgac tcacaagaaa cgctcgcgct ggcggtaacg aaacatcatc agcaggcact
    15541 ggaaaccgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    15601 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga ttgatgtcaa
    15661 atatgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    15721 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    15781 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    15841 tgatgaagtg gatgtaggga ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    15901 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    15961 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacacatat
    16021 gcaatctctg gataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    16081 agtcacacgt aacacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    16141 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    16201 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    16261 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    16321 tgttaaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    16381 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    16441 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    16501 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    16561 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    16621 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    16681 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    16741 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    16801 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    16861 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcactc gctgcaggta
    16921 ttgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctagca ctgtaaacct
    16981 ctttcgcacg aaccgtaaaa gcctggacca tattagccgc cagctcttta aacacgcgac
    17041 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    17101 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    17161 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    17221 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    17281 gaatccgact tccggtacaa cctggcaaaa actgaggata agactgaacg tcattcacta
    17341 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    17401 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    17461 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    17521 tgatatgggg tattttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    17581 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    17641 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaagt taaatccctg
    17701 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    17761 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    17821 acccgtaccc gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgtc
    17881 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    17941 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    18001 cgcgaatggc aggtggataa agcacgcatc atgaaaaacg cccaccgtta aacctgcact
    18061 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    18121 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    18181 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    18241 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    18301 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    18361 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    18421 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    18481 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    18541 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    18601 gcgggttcaa ctcccgccag ctccaccaaa attctccatc ggtgattacc agagtcatcc
    18661 gatgaagtcc taagagcccg cacggcgcaa gccctgcggg cttttttgtg ctccgaattt
    18721 gtcccgcgaa gtctgatgcc aactaattaa atccgatcct tttaggcacc ttgttaggca
    18781 cttcataaag ctatattgtc tttgaggtgc ctaaaactat ggaaacccgg caatggcaag
    18841 acaaaccaaa cctctatccg ttaaggaaat cgaatctgct aaacccaagg aagcggacta
    18901 cgttctctat gatggcgatg gccttgagct actcattaaa tccagcggga gtaaaatctg
    18961 gcagtttcgc tacattcgcc ctgtcaccaa gacacgagca aagaaaagca taggccctta
    19021 cccgtcagtt acccttgccg atgccagaaa ctaccgtgca gagtcccgct cactcctagc
    19081 taaacagatt gatccgcagg aacatcagca agaacaattg cgtagttcgc tggaagccaa
    19141 aaccaatact ttccagctcg tagctgaacg ttggtggaat gtgaagaaag ccagtgtgac
    19201 agaggactat gcggaagata tctggcgctc tcttgaaaga gatgtctttc ctgcgattgg
    19261 cgacgttagc gttacagata ttaaagctca tacactggtt caggccgtac aaccggttca
    19321 ggccagagga gcactggaaa cggttcgtcg cctgtgtcaa cgtattaatg aggtcatgat
    19381 ctatgcccag aacacagggc tgattgatgc tgttcccagc gttaatatcg gtaaagcctt
    19441 cgaaaagcct cagaaaaaga acatgcccag cattcgaccg gatcagctat ctcaactgat
    19501 gcagacaatg cgaacagcca gcattagcct ttccacacgc tgcctgttca tgtggcaact
    19561 tcttactatt acccgccctg ccgaagcggc tgaagctcgc tgggaagaag tagacataga
    19621 agcgcaagag tggaagattc ccgcagcacg catgaaaatg aaccgcgacc atactgttcc
    19681 attgtcagat gaagcaatag ctgtgctgga gatgatgaaa ccgctaagcg gcaaccgaga
    19741 atttatcttt cctagccgta tcaagcccaa ccagcctatg aacagtcaga ctgttaacgc
    19801 ctcactaaaa cgcgcaggtt ttggtggtgt gcttgtttcg catggtctga gatcaattgc
    19861 cagtacggct cttaatgaac aaggctttcc gcctgatgcc attgaggcag cattggccca
    19921 tgtggataag aatgaggttc gtcgtgctta taaccgcagc gattacctgg aacagcgtcg
    19981 cccgatgatg caatggtggg caaattttgt gatggcagcg gatcgtggaa gcatgattga
    20041 aggtgggata aaaggaatga agctagttgg gtgactaaat agctgcgcgg catagtgtcc
    20101 cactttcagc cagcccttgc aggttaagcc accagacagt aaacggcggg tgtcgcgtta
    20161 ttaggcgcag ctatttagta gtaatattag gattggtgat tctgcactcc agaagttcgg
    20221 taatgctgcc aacttact
//
LOCUS       DAJTNP010000074        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTNP010000074 DAJTNP010000000
VERSION     DAJTNP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO160_002820"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO160_002820"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5524871.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO160_002821"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO160_002821"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5524872.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO160_002822"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO160_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5524873.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO160_002823"
     CDS             6038..6658
                     /locus_tag="OO160_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5524874.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO160_002824"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO160_002824"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5524875.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO160_002825"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO160_002825"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5524876.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO160_002826"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO160_002826"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5524877.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO160_002827"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO160_002827"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5524878.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO160_002828"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO160_002828"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO160_002829"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO160_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO160_002830"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO160_002830"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5524879.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO160_002831"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO160_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5524880.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO160_002832"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO160_002832"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5524881.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO160_002833"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO160_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5524882.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO160_002834"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO160_002834"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5524883.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO160_002835"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO160_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5524884.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4682 a         5506 c         5032 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTNP010000075        19369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTNP010000075 DAJTNP010000000
VERSION     DAJTNP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO160_002836"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO160_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5524885.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO160_002837"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO160_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5524886.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO160_002838"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO160_002838"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5524887.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO160_002839"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO160_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5524888.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO160_002840"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO160_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5524889.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO160_002841"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO160_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5524890.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO160_002842"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO160_002842"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5524891.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO160_002843"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO160_002843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5524892.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO160_002844"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO160_002844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5524893.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO160_002845"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO160_002845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5524894.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO160_002846"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO160_002846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5524895.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO160_002847"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO160_002847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5524896.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO160_002848"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO160_002848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5524897.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO160_002849"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO160_002849"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5524898.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO160_002850"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO160_002850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5524899.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO160_002851"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO160_002851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5524900.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO160_002852"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO160_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5524901.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO160_002853"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO160_002853"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5524902.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4770 a         4851 c         5117 g         4631 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acagacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatatgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaaggg
//
LOCUS       DAJTNP010000076        18803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTNP010000076 DAJTNP010000000
VERSION     DAJTNP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(139..3654)
                     /locus_tag="OO160_002854"
     CDS             complement(139..3654)
                     /locus_tag="OO160_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5524903.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO160_002855"
     CDS             complement(4107..5369)
                     /locus_tag="OO160_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5524904.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO160_002856"
     tRNA            complement(5567..5642)
                     /locus_tag="OO160_002856"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO160_002857"
     CDS             complement(5748..6455)
                     /locus_tag="OO160_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5524905.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO160_002858"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO160_002858"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5524906.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO160_002859"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO160_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5524907.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO160_002860"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO160_002860"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5524908.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO160_002861"
     CDS             complement(11639..11914)
                     /locus_tag="OO160_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5524909.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO160_002862"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO160_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5524910.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO160_002863"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO160_002863"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5524911.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO160_002864"
     CDS             13874..14200
                     /locus_tag="OO160_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5524912.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO160_002865"
     CDS             14384..15103
                     /locus_tag="OO160_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5524913.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO160_002866"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO160_002866"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5524914.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO160_002867"
     CDS             16442..17404
                     /locus_tag="OO160_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5524915.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OO160_002868"
     CDS             complement(17505..>18803)
                     /locus_tag="OO160_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5524916.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJTNP010000077        18218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTNP010000077 DAJTNP010000000
VERSION     DAJTNP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..1238
                     /locus_tag="OO160_002869"
                     /pseudo
     CDS             1..1238
                     /locus_tag="OO160_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="OO160_002870"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="OO160_002870"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5524917.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="OO160_002871"
     CDS             1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="OO160_002871"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5524918.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="OO160_002872"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="OO160_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5524919.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="OO160_002873"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="OO160_002873"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5524920.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="OO160_002874"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="OO160_002874"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5524921.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="OO160_002875"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="OO160_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5524922.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="OO160_002876"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="OO160_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5524923.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="OO160_002877"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="OO160_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5524924.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="OO160_002878"
     CDS             10439..10654
                     /locus_tag="OO160_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5524925.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="OO160_002879"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="OO160_002879"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5524926.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="OO160_002880"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="OO160_002880"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5524927.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="OO160_002881"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="OO160_002881"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5524928.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="OO160_002882"
     CDS             complement(15390..15929)
                     /locus_tag="OO160_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5524929.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..16463)
                     /locus_tag="OO160_002883"
     CDS             complement(15945..16463)
                     /locus_tag="OO160_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5524930.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16774..16965)
                     /locus_tag="OO160_002884"
     CDS             complement(16774..16965)
                     /locus_tag="OO160_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5524931.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            16983..17135
                     /locus_tag="OO160_002885"
     CDS             16983..17135
                     /locus_tag="OO160_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5524932.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17317..>18218
                     /gene="pdeF"
                     /locus_tag="OO160_002886"
     CDS             17317..>18218
                     /gene="pdeF"
                     /locus_tag="OO160_002886"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5524933.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSS"
BASE COUNT         4318 a         4425 c         4931 g         4544 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacattttt taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt
    16501 taagatcatc aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt
    16561 aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat attaatttta
    16621 tatttatttc gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga
    16681 tgaaagaata tctgccgctt tgataatccc aaagagcaaa agcatccgac agcgaagtgc
    16741 cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga
    16801 gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga
    16861 gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg
    16921 actgttaaat cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag gttccttgaa
    16981 gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt
    17041 cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa
    17101 ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa
    17161 tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat
    17221 gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca
    17281 cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata
    17341 aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt
    17401 ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg
    17461 cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc
    17521 accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta
    17581 acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc
    17641 cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag
    17701 atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta
    17761 ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat
    17821 tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg
    17881 gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat
    17941 gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg
    18001 ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca
    18061 ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct gatttcgctg
    18121 ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat
    18181 cctggctata ccacgcagct gaccataacc tcctccag
//
LOCUS       DAJTNP010000078        18052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTNP010000078 DAJTNP010000000
VERSION     DAJTNP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            446..1129
                     /locus_tag="OO160_002887"
     CDS             446..1129
                     /locus_tag="OO160_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5524934.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO160_002888"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO160_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO160_002889"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO160_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5524935.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO160_002890"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO160_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5524936.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO160_002891"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO160_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5524937.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO160_002892"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO160_002892"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5524938.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO160_002893"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO160_002893"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5524939.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO160_002894"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO160_002894"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5524940.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO160_002895"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO160_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5524941.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO160_002896"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO160_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5524942.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO160_002897"
     CDS             complement(13386..13817)
                     /locus_tag="OO160_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5524943.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO160_002898"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO160_002898"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5524944.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO160_002899"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO160_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5524945.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO160_002900"
     CDS             16903..17862
                     /locus_tag="OO160_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5524946.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18052)
                     /locus_tag="OO160_002901"
                     /pseudo
     CDS             complement(17849..18052)
                     /locus_tag="OO160_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4548 a         4663 c         4674 g         4167 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaat
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg ca
//
LOCUS       DAJTNP010000079        17719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTNP010000079 DAJTNP010000000
VERSION     DAJTNP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..433
                     /locus_tag="OO160_002902"
     CDS             <1..433
                     /locus_tag="OO160_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5524947.1"
                     /translation="IKGNIAESPCKFEAGGDSVSINMPTVPTTVFEGKAKYSTYDDAV
                     GVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGDNSDVLDVSAPFNIE
                     SYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
     gene            514..1188
                     /locus_tag="OO160_002903"
     CDS             514..1188
                     /locus_tag="OO160_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5524948.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1204..3684
                     /locus_tag="OO160_002904"
     CDS             1204..3684
                     /locus_tag="OO160_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5524949.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3700..4733
                     /locus_tag="OO160_002905"
                     /pseudo
     CDS             3700..4733
                     /locus_tag="OO160_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4815..5153)
                     /gene="rcnB"
                     /locus_tag="OO160_002906"
     CDS             complement(4815..5153)
                     /gene="rcnB"
                     /locus_tag="OO160_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5524950.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5372..6193)
                     /gene="rcnA"
                     /locus_tag="OO160_002907"
     CDS             complement(5372..6193)
                     /gene="rcnA"
                     /locus_tag="OO160_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR5524951.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRGERNWLENMHGHDYEHHHHHHDHEHHHDQGHHHHHEHGEYQDAHARAHANDI
                     KRRFDGREVINWQILLFGLTGGFIPCPAAITVLLICIQLKALTLGATLVVSFSIGLAL
                     TLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6314..6586
                     /gene="rcnR"
                     /locus_tag="OO160_002908"
     CDS             6314..6586
                     /gene="rcnR"
                     /locus_tag="OO160_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5524952.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6678..6774
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6809..7597
                     /gene="thiM"
                     /locus_tag="OO160_002909"
     CDS             6809..7597
                     /gene="thiM"
                     /locus_tag="OO160_002909"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5524953.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7594..8394
                     /gene="thiD"
                     /locus_tag="OO160_002910"
     CDS             7594..8394
                     /gene="thiD"
                     /locus_tag="OO160_002910"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5524954.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8459..9280
                     /locus_tag="OO160_002911"
     CDS             8459..9280
                     /locus_tag="OO160_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5524955.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9328..10074
                     /locus_tag="OO160_002912"
     CDS             9328..10074
                     /locus_tag="OO160_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5524956.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10048..11013)
                     /locus_tag="OO160_002913"
     CDS             complement(10048..11013)
                     /locus_tag="OO160_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5524957.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11010..12013)
                     /locus_tag="OO160_002914"
                     /pseudo
     CDS             complement(11010..12013)
                     /locus_tag="OO160_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12053..13105
                     /gene="fbaB"
                     /locus_tag="OO160_002915"
     CDS             12053..13105
                     /gene="fbaB"
                     /locus_tag="OO160_002915"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5524958.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13413..14267
                     /gene="gatY"
                     /locus_tag="OO160_002916"
     CDS             13413..14267
                     /gene="gatY"
                     /locus_tag="OO160_002916"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5524959.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14296..15558
                     /gene="gatZ"
                     /locus_tag="OO160_002917"
     CDS             14296..15558
                     /gene="gatZ"
                     /locus_tag="OO160_002917"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5524960.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15568..16020
                     /gene="gatA"
                     /locus_tag="OO160_002918"
     CDS             15568..16020
                     /gene="gatA"
                     /locus_tag="OO160_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5524961.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16051..16335
                     /gene="gatB"
                     /locus_tag="OO160_002919"
     CDS             16051..16335
                     /gene="gatB"
                     /locus_tag="OO160_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5524962.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16339..17694
                     /locus_tag="OO160_002920"
     CDS             16339..17694
                     /locus_tag="OO160_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5524963.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4612 a         4062 c         4526 g         4519 t
ORIGIN      
        1 cattaaagga aatattgcag aatctccgtg taaattcgaa gcgggtggtg attcagtaag
       61 tattaatatg ccgactgtac caaccactgt ctttgaaggt aaagctaaat attctaccta
      121 tgatgatgca gtcggtgtaa ccagcagcat gttaaaaatt agctgcccga aagaagttgc
      181 tggtgtaaaa ctctcgttga tcacaaacga caaaataacc ggtaacgata aggcgatagc
      241 cagtagcaac gataccgtag gtgataacag cgatgtccta gatgtttctg caccttttaa
      301 cattgagagt tataaaacag cggaaggtca atatgctatt ccgtttaaag caaaatacct
      361 gaaactgaca gataactcag tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt
      421 ggcgcaggat taataaataa aattgcaagt ttctccaggt aataaacatg gccgctatcc
      481 ccatggcggc tttttaattt aagaggcatt aagatgaaag gattattatc tttactcatt
      541 ttttctatgg tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac
      601 ccggcagaaa ataaagaagt tatggtgcag ttgatgaacc agggaaatcg ttcttcgctg
      661 ctgcaggcgt ggattgatga tggcgatacg tcgttaccac cagaaaaaat tcaggttcct
      721 ttcatgttaa cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc
      781 aaaattatgc cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg
      841 tatattccac caaatagtcc agagcaagaa ggcaagaatg cactgaagtt tgcgatgcaa
      901 aacagaatta agttgtttta ccggccagcg ggtattgctc cggtaaataa agcgaccttt
      961 aaaaagttac tggtaaatcg cagtggcaat ggattggtga taaaaaatga ctcagctaat
     1021 tgggtgacga tttcggatgt caaagctaat aatgttaaag tcaattatga aactattatg
     1081 attgccccct tagaaagtca gagtgttaat atcaaaagta ataatgcaaa taactggtat
     1141 ctgaccatta tcaatgacca cggcaactat attagtgaca aaatttaatt gcaggagctg
     1201 cctatgttga gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa
     1261 gcttatgcag ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag
     1321 caggttgcaa atattcgtct tgatgataat caacccttac cggggcagta tgacatcgat
     1381 atttatgtca ataagcaatg gcgcgggaaa tatgagatta ttgttaaaga caacccgcaa
     1441 gaaacatgtt tatcaagaga agttatcaag cggttaggca ttaatagcga taacttcgcc
     1501 agcggtaagc aatgtttaac atttgagcaa cttgttcagg gtgggagcta tagctgggat
     1561 atcggggttt ttcgtctcga tttcagtgtc ccgcaggctt gggtggaaga actggaaagt
     1621 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ttcttattat
     1681 gtgagtcagt attacagcga ctataaagcg tcgggtaaca acaagagtac atatgtacgt
     1741 tttaacagcg ggttaaattt actggagtgg caactgcatt ctgatgccag tttcagtaaa
     1801 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
     1861 caatttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
     1921 gttcgcttca gcggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
     1981 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
     2041 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
     2101 cagttggccg gtggtggagc agatcttgat gttagcgtga aagaggcgga cggctcggta
     2161 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
     2221 tatgattttg cggcgggccg tagccatatt gaaggggcga gcaagcaaag tgattttgtc
     2281 caggcaggtt atcagtatgg ttttaataac ttattgacgc tgtatggcgg cacgatggtt
     2341 gctaataatt actatgcgtt taccctcgga acgggttgga acacgcgcat tggcgcaatt
     2401 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
     2461 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
     2521 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
     2581 aaagataatt atcgccgtga tgaaaacgat atctatgaca ttgccgatta ttaccagaac
     2641 gattttggcc gtaaaaatag cttttctgcc aatatgagcc agtcattgcc agaaggctgg
     2701 ggttctgtgt ccttaagtac gttatggcga gattactggg ggcgtagcgg cagcagtaag
     2761 gattatcagt tgagttattc caataactgg cggcggataa gctataccct cgcggcaagc
     2821 caggcgtatg gcgagaatca tcatgaagag aaacgtttta atatttttat atcaattccc
     2881 tgtgattggg gtgatgacgt tacgacgcct cgtcggcaaa tatatatgtc taactcaacg
     2941 acgtttgatg atcagggttt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     3001 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     3061 gctggggcga atttgacctg gaacgcgccg gttgcgacag tgaatggcag ttatagtcag
     3121 tcgagtactt atcgacagac tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     3181 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     3241 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     3301 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     3361 agcgaagcag aattacgtgg caaccggaaa attgccgccc cttatcgcgg cgcggttgta
     3421 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     3481 ggtcaaccat taacgtttgg ttatgaagtc aatgatatcc atggtcataa tattggcgtt
     3541 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     3601 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     3661 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     3721 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     3781 gttcagcaca atttggttgt tcaggggaat tttgcactta ctcaaacaca aatagcgaca
     3841 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagc
     3901 ccatcggata ttattgttgg actttataac gataccatta aattaaattt acattttgaa
     3961 tggaccaata aaaacaacat cacgttgtca aataatcaga gcagtttcac cagtggttat
     4021 tcggtaacgg tgacacctgc ggccagtaat gcaaaagtga atatttctgc ggggggcggt
     4081 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     4141 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatgggg tgcatgtgta
     4201 aatagctaca gaaatgcatt ggcacaaaat gcaggtgtct attccttcaa tctgacattg
     4261 tcatacaacc cgataaccac aacctgcaaa caggacgatt tattaattac tttagacagt
     4321 attcccgttt tacaattacc agccacaggt aacaaagcaa caataaatag taaaaaaagg
     4381 gatattattc tgcgttgtaa aaatttatta ggtcaacaaa atcaaacatc acggaaaatg
     4441 caggtgtatt tatcaagttc tgacttgtta accaacagca acacaatact gaaaggtgcg
     4501 gaagataatg cgtaggattt attcttgaaa gtaatggttc gccagtcaca cttttaaata
     4561 tcactaacag cagtaaagga tacacaaatt taaaagaaat tgcggcaaag tcaaaactta
     4621 cagatacaac ggtttcaatt ccgataacag ccagttacta cgtctacgat acaaacaaag
     4681 ttaaatctgg cgcactggag gcaaccgcat taatcaacgt gaaatacgac taataacaag
     4741 caaaacgagc aagtggctaa taataaaaaa taacaaggtg tctgcatttc cccctcatga
     4801 tgagggggct ttttttagcg atgataaaaa atctcaccgt cgtaggcttt aatgatttta
     4861 ccgtcggtgt cgctgatcaa cacgtacgcg ccacccatat aggtccagtg cgtcccggca
     4921 tcaggcgcgg gcaggttacg taactgccac tgcttaatgt tgtactcatc ggtgcggtac
     4981 atctctggga cggtatcacc tggtttgaaa tgcttaaagt cagcaatgaa actacttaac
     5041 tcatacttag aaattcccga ggtattggtc gaacccgcgg tggctggtgc ggcccaggcg
     5101 gcactggtaa ccagcaaaag cacacccagc aacatcttat ttttaatggt cataaactct
     5161 ccactttggg gatctgtaat atcagtcgca tatctttcct gtcatacaat ataactgaca
     5221 agcgatattt tgttagtgat cccatgattt cgtaaaaagt gtaaatgaaa ggatgcccta
     5281 aaagcatcct tagcgagcag agagtcagcg gaaatagctg atttaagaat catcctgggc
     5341 tgaagataac atgcaagcct aaaggattga gttatcgcat tatgcccatg aagccatgta
     5401 cgcccatata cacaccgact aagccaatca acagactgga aaaatagggg gcgcgtttag
     5461 cgagagtgtt aaatccgctc cagcgttttg cgacctgctg aacgctgatt gctgcgccaa
     5521 cgcctacggt gacaagcgtt aacgccaggc caatgctgaa actgacgacc agtgttgcgc
     5581 ccagtgtcag ggctttcaac tgaatgcata tcaacagcac ggtaattgct gccgggcagg
     5641 ggataaagcc accggttaag ccaaataaca aaatttgcca gttgatgacc tctctaccat
     5701 caaagcgtcg tttaatgtca ttggcatggg ctcgtgcatg ggcatcctga tactcgccat
     5761 gttcgtgatg gtgatgatgt ccttggtcgt ggtgatgttc gtgatcgtga tgatggtgat
     5821 gatgctcata atcatgcccg tgcatattct ccagccagtt gcgttcgccg cgccagaaca
     5881 tccagaacgc ggtgccaata atgatcactg cggaaatcag ctggagccac ggttctgctg
     5941 attgagcagt aaagcgctta ctgatcacca tcccgccaaa ggcaatcaac cagaccaccg
     6001 cggtatgcga aatggttgct gccagtccga gcatcaccgc ctgtttaatg gtgcctttga
     6061 tggcgatgat aaacgccgcc atcatcgttt ttgagtgccc tggttccagg ccatgaagcg
     6121 caccaagtaa gatggcgctg gggatgaaga accaggcgtt tccttgctga agaagagttg
     6181 taaattcggt catgataata attcttagta ttaattcggc aatctgattc tactcccccc
     6241 cagtacctga tactaccccc cagtagatta atagtgctat gatttttcat attcttgtta
     6301 accaggtgtt gccatgtctc atacaatccg tgataaacag aaactgaaag cgcgtgccag
     6361 taagattcag ggccaggtcg tggcgctcaa gaaaatgctc gacgagccgc acgaatgcgc
     6421 tgcagtttta caacagattg ctgctatccg tggagcggta aacggtctga tgcgggaagt
     6481 gattaaaggt catctgacgg aacacatcgt tcaccagggg gatgagctaa aacgtgaaga
     6541 agatctggat gtcgttctga aggtgctgga ttcatatatc aaataattta ttaacgcgat
     6601 tgtaaaactg ccgtttttcc tcgtttacaa cgggggcgct ggacattacc atcctcctct
     6661 gcgatttatc atcgcaacca aacgactcgg ggtgcccttc tgcgtgaagg ctgagaaata
     6721 cccgtatcac ctgatctgga taatgccagc gtagggaagt cacggaccac caggtcattg
     6781 cttcttcacg ttatggcagg agcaaactat gcaagtcgac ctgctgagtt cagcgcaatc
     6841 tgcgcacgcg ttacaccttt ttcaccaaca ttcccctctt gtgcactgca tgaccaatga
     6901 tgtggtgcaa acctttaccg ccaatacctt gctggcgctc ggtgcatcgc cagcgatggt
     6961 tatcgaaacc gaagaggcca gtcagtttgc ggctatcgcc agtgccttgt tgattaacgt
     7021 tggcacactg acgcagccac gcgcacaggc gatgcgtgct gccgttgagc aagcaaaaag
     7081 ctctcaaaca cgctggacgc ttgatccagt agcggttggt gcgctcgatt atcgccgcca
     7141 tttttgtcat gaacttttat cttttaaacc ggcagcgata cgtggtaatg cttcggaaat
     7201 catggcatta gctggcattg ctaatggcgg acggggagtg gataccactg acgccgcagc
     7261 taacgcgata cccgctgcac aaacactggc acgggaaact ggcgcaatcg tcgtggtcac
     7321 tggcgaggtg gattatgtta ccgatggaca tcgtatcatt ggtattcacg gtggcgatcc
     7381 gttaatgacc aaagtggtag gaactggctg tgcattatcg gcggttgtcg ctgcctgctg
     7441 tgcgttacca ggcgatacgc tggaaaatgt cgcatctgcc tgtcactgga tgaaacaagc
     7501 cggagaacgc gcagtcgcca gaagcgaggg gccaggcagt tttgttccac atttccttga
     7561 tgcgctctgg caattgacgc aggaggtgca ggcatgaaac gaattaacgc tctgacgatt
     7621 gccggtactg atccgagtgg tggtgcgggg attcaggccg atcttaaaac cttctcggca
     7681 cttggcgctt atggttgctc agttattact gcactggtgg cgcaaaatac ccgtggcgta
     7741 cagtcggtgt atcgcattga gcctgatttt gtcgccgccc agctcgattc ggtgttcagc
     7801 gatgtgcgaa tcgataccac taaaatcggt atgttggcgg aaaccgatat tgttgaagcg
     7861 gtggcagaac ggttgcaacg ttatcagatc caaaacgtgg tactcgacac cgttatgctg
     7921 gcaaaaagcg gcgacccgct gctttcacct tcggcggttg ctacgctgcg cagtcgatta
     7981 ttgccacagg tttcattaat aacgccaaac ttgcccgaag ctgccgcctt gctcgacgcg
     8041 ccacacgcgc gcaccgaaca ggaaatgctg gaacaagggc gatcgctgtt ggcgatgggc
     8101 tgtggcgcag tgctaatgaa aggtggtcat ctggatgatg agcaaagccc ggactggctg
     8161 tttacccgcg agggtgaaca acggtttacc gcaccgcgca ttatgaccaa aaacacccac
     8221 ggcactggtt gtacactctc tgcggcgttg gctgcactac gcccgcgcca tacaaactgg
     8281 gctgacaccg tacaggaggc aaaaatctgg ctttcatcgg cgttagccca ggccgacacg
     8341 ctggaagttg gtcacggtat tggtccggtt caccacttcc acgcctggtg gtgagcaccg
     8401 tactatagct gctttggtta ttgtcagcct ttggcactga cattcaggga tgaaaccaat
     8461 gcagctaaga attaccagtc gaaaaaaact caccgctcta ttatgcgctc ttgggctaat
     8521 ttccatcgtt gcaatttatc cgcgtcagac ggtgaatttt ttctattcga cagcagttca
     8581 gattacagac tacatccact tctacggtta tcgcccggtt aaatctttcg ctattcgcat
     8641 tcctgccagt tacaccattc acggattaga tgtttcgcgc tggcaggagc gtatcgactg
     8701 gcagcgggtg gcaaaaatgc gcgacaacga tatccgctta cagtttgctt ttattaaggc
     8761 tacggaaggc gaaaagctgg tggaccccta tttttcgcgt aactggcaat taagccgcga
     8821 aaatggcctg ctgcgcgggg cgtatcatta tttctccccg tcggtatccg cttcagttca
     8881 ggcgcgatta tttctgcaaa cggtggattt ctcacaaggc gatttccctg ccgtgctgga
     8941 cgtagaagaa cggggaaaat tatcggcaaa agaattacgc aagcgggtaa gtcagtggct
     9001 aaaaatggtc gaaaaacgca cgggaaaaaa gccgattatt tactcaggag ccgtttttta
     9061 tcacaccaat ctggcgggct atttcaatga atatccgtgg tgggtggctc actattatca
     9121 acgtcgtccg gacaatgacg gcatggcctg gcgcttctgg cagcattccg accgtggaca
     9181 ggtagatggc atcaatggtc cggtggattt taatgtgttt aatggcacgg ggatgagttg
     9241 caggcattcg ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca
     9301 ccagactccc tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa
     9361 ctgaacgagc gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg
     9421 gtaaaaaatg ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt
     9481 gatttaagtc agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg
     9541 gaagaagagg gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc
     9601 gaatactcgt tgaaagaagc gagaggtttt tctcagcagg tggtattgcg cgggaaaaag
     9661 cccgatacgc tatgggttaa taagcgtgtc gtgaaatgtc ccgaagaagt tgcgcagcag
     9721 ctggcggtcg aagcgggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa
     9781 gaagcggtat cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc
     9841 atcggaattt ctctttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt
     9901 tcccgcgtta gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc
     9961 aaaataccgg tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag
    10021 tacagcatta gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc
    10081 gcgcgttggc gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc
    10141 cagtaatacg gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc
    10201 atgactgtcg cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc
    10261 agaagcgtcg ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc
    10321 ggcaaatttc tcctgccatt gcttgccaag tgttgttatc tctgcggata aagcaaaacg
    10381 ttcggcggca atctcagcct cttgacgatt gagtgacact aaaggtcgac aggccatgat
    10441 ccgcgccagt aatgcgtccg ggatatcgcc aatacgtggg caaaaatcga taaacggcgt
    10501 cacgtcttgc agcttttcca gccattccac taacaattcg ccgcagggcg aggccagttg
    10561 ataaccggaa aaatagagca ggttgccagg cgcaacggtt aatcgtgcca accattggcg
    10621 attccattga ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac
    10681 cagtgccaga caccagccgt tatcgctttc ggcgttatcg atcaggctga ttaacccctc
    10741 tttcgccatc cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt
    10801 acccgcttcg atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac
    10861 gttaacgctc tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc
    10921 ggcgatcacg tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc
    10981 aggcagcagt gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc
    11041 gccagcgctg tggcatagcg gttgaaatca agctgattta ccgcatccag ctccgccttt
    11101 aacgcaggat cgacagcgtt aatgccatgc aacgcgccgc aaatcgccgt cgccatagca
    11161 cctatggtgt ctgtgtcgcc gccaagattg gcgcacagga cggcgcagcg gtttgggtcg
    11221 gtttgtgcca gttcaactag cgcaatggcg cacggaacgg actcaatagt gctggtgcct
    11281 gcgccaacga cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attacgcaca
    11341 attttcagcg caatctccag acgtgccgcc agtgaggcgc tgaacgtggt ggtgcgtttt
    11401 tgctgtgcat gtcgcgcaat tgaaggcaat gaatcaacaa tcgctgacca gcgttctccg
    11461 tcaatggcac gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat
    11521 ttatgggtcg ggctggacgc cagcgccaca tcatcaataa aggaatcaac atcacgcgct
    11581 ggaagcaaac aacctaatgg cgagacgcgc atcgctgcgc cgttggttac gccgttgttt
    11641 tccagttcag caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga
    11701 cctagtacgt ttttgttaaa ggcgtcgaaa cgcaacgccc agtcgagaat attacgccca
    11761 atcagattcg gatcgatctt gccttcacat tccagtaacg catccgccag acacaggcca
    11821 tcgaggtatc gtcggtgaat tcggcgcggt taaaataaca ggccgcgtta ttctcctttg
    11881 gtccaggcag aaaacggtca atccagccaa agtgtgcttt aacgcggctg cgtggccaaa
    11941 gctcggaggg catccccatc gcatccccta acgcctgccc ataaagagca ccgagaatac
    12001 gttctgtttt catttaactt ccccttgaat cggaatttac gggagcatag taatgacaga
    12061 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgactat
    12121 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtga tgattgacaa
    12181 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    12241 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    12301 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    12361 agcgggctgt aactgtgtgg catcaactta cggcgtgttg gtgtcggtat cgcggcgcta
    12421 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    12481 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    12541 ggttggtgcg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    12601 tgcggccttt gaacgcgcgc acgagctggg catggtgaca gtgctgtggg cctatttgcg
    12661 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgactggtca
    12721 ggcgaaccat ctggcggcaa ccataggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    12781 taacggcggc tataaagcga ttaattacgg ttataccgac gatcgcgtgt acagcaagtt
    12841 aaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    12901 ggctgggttg ataaactccg gcggtgctgc aggcggtgaa actgacctca gcgatgcagt
    12961 gcgtactgcg gttatcaaca aacgcgcagg cggaatgggg ctgattcttg gacgtaaggc
    13021 gttcaagaaa tcgatggctg acggcgtgaa actgattaat gccgtgcagg acgtttatct
    13081 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13141 gataagacgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    13201 catgcctaca tagcactgcc acgtatgttt acaccgcatc cggcataaaa acacgcgcac
    13261 tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc gaacaatttt
    13321 catattgttg tttttgcgat cattatctcg acatttaaaa gcaaataatt tcattatatt
    13381 ttgaaatcga aagcaaacga caagatatga aaatgtacgt ggtatcgaca aagcagatgc
    13441 tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac aatctcgaaa
    13501 cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc atcgccggaa
    13561 cgcctggcac atttactcat gctggtacag aaaatctgtt ggcgctggtc agcgcgatgg
    13621 cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa tttgacgata
    13681 tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt
    13741 ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc tttgatgtca
    13801 gcgtcgaggc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag
    13861 tcgatgcgtt gtacaccaac cccgcacagg cgcgtgaatt tgccgaggca accgggattg
    13921 attccctggc ggtcgccatc ggcacggctc acggtatgta tgccagcgca ccggtgcttg
    13981 atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg ctgcatggcg
    14041 cgtcaggatt atcgactaag gatattcagc aaaccatcaa actggggata tgcaaaatca
    14101 acgttgcaac ggagctgaaa aatgccttct tgcagtcgtt aaaaaattac ctgaccgagc
    14161 accccgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca atgcgcgatg
    14221 tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc acctgccatt
    14281 taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga acatatcggc
    14341 atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc atttgatcgc
    14401 aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca atttggcggt
    14461 tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc cgataaagtt
    14521 gggtttgcac gcgaacgcat tattctcggc ggcgatcatc tgggaccaaa ctgctggcag
    14581 caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt
    14641 gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga taccataccg
    14701 ttagcaccag aaacggttgc ggaacgagct gctgtgcttt gctttgctgc ggaaagtgtg
    14761 gcgacagatt gccagcgtga gcaactgagc tatgtcattg gcactgaagt tccggttccg
    14821 ggcggtgagg ccagcgccat tcagtcagta cacatcaccc atgttgaaga tgccgccaat
    14881 actttacgta cgcatcaaaa ggcctttatt gcccgtgggc tgacagaggc gttaacacgt
    14941 gtgattgcca tcgtggtgca gccgggtgtg gaatttgatc acagcaatat tatccattat
    15001 cagccgcagg aagcgcaggc gctggcgcaa tggatagaaa acacccgaat ggtttatgaa
    15061 gcacattcta ccgattacca gacccggacg gcttattggg aattagtccg cgatcacttt
    15121 gcaatattga aagtcggtca cgcattaacc tttgctttac gcgaggcgat atttgcactg
    15181 gcacaaattg agcaggaact tatcgccccc gaaaatcgca gcggttgcct ggcggtaatt
    15241 gaagaagtga tgctcgacga accgcaatac tggaaaaaat attatcgcac gggttttaac
    15301 gattcattac tggatattcg ttacagcctg tcggatcgta ttcgttatta ctggccgcat
    15361 agtcggatta aaaatagcgt tgaaacgatg atggtgaatc tgcaaggcgt ggacatccca
    15421 ctgggcatga ttagtcagta tcttcccaaa caatttgaac gcattcagtc cggggaatta
    15481 tcagcaatgc cgcatcagct tattatgaat aaaatttatg atgttttgcg cgcctatcgc
    15541 tacggctgtg cggaataagg acggtatatg actaacctgt ttgttcgtag cggaatttct
    15601 tttgtcgatc gtagcgaagt tttaacctat atcggtaatg agatgctcgc gaaaggtgtg
    15661 gttcatgata cctggccaca ggcattaatt gccagagaag cagaattccc taccgggata
    15721 atgcttgagc agcacgccat tgcaataccg cattgtgagg cgattcatgc taagtcgtca
    15781 gccatttatc tgttaaggcc aacaaataaa gttcattttc agcaagcgga tgatgataac
    15841 gacgtggcgg tatcgttggt tattgcgttg attgtggaaa atccgcagca gcaattgaaa
    15901 cttttacgct gtttatttgg caagttacaa cagcccgata tcgtcgagac actaattact
    15961 cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg ttttagattc agacgaataa
    16021 atccctctgt aacaataaaa aggactattt atgaaacgca agattattgt cgcttgcgga
    16081 ggcgcggttg cgacctctac gatggcggcg gaagaaatta aagagttgtg tcagagtcat
    16141 aatattcctg ttgaattaat ccagtgtcgg gttaatgaaa tagaaaccta tatggatggt
    16201 gtgcatttga tatgtaccac tgccagggtg gatcgtagtt ttggcgatat tccgttagtt
    16261 cacggcatgc cttttgtttc tggtgtcggt atcgaagcat tacaaaataa aattctgact
    16321 atcttacagg gatgacctat gttttcagaa gtcatgcgtt atattctcga cctcggccct
    16381 acagtgatgc tgccggttgt catcattatt ttttctaaaa tattaggcat gaaggcaggc
    16441 gattgcttta aagcgggtct gcatatcggg attggctttg ttggcattgg ccttgtgatt
    16501 ggcttaatgc tggattccat tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg
    16561 aatctgcatg tggtcgatgt cggctggccg ggctcttcac caatgacctg ggcgtcgcaa
    16621 attgcgctgg tggcgattcc gattgcgata ttggttaacg tggcgatgtt actgacccgt
    16681 atgacgcggg tggtaaatgt tgatatctgg aatatctggc atatgacctt caccggcgcg
    16741 ttgctgcacc tggcaaccgg ttcatggatg atagggatgg cgggtgtggt aattcacgcg
    16801 gcgtttgttt ataagctcgg cgactggttt gcccgcgata cccgaaattt ctttgagctg
    16861 gaaggtattg ctattccgca cggtacgtcg gcgtatatgg ggccgattgc ggtgctggtc
    16921 gatgctatca tcgagaaaat cccaggcgtt aaccgaatta aatttagcgc cgacgatatt
    16981 cagcgcaaat ttggcccttt tggcgaacct gtcaccgtgg gttttgtgat ggggctgatt
    17041 atcggcatcc tcgcgggtta cgatgtcaaa ggtgtattgc agctggcggt aaaaacggcg
    17101 gcagttatgc tgctgatgcc acggatgatt aaacccatca tggatggttt aacgcccatc
    17161 gctaagcagg cccgtagtcg tttacaggcg aagttcggcg gtcaggagtt cctgattggc
    17221 ctggatcccg cattactgct ggggcatacg gcggtggtat cggcaagcct gatttttatt
    17281 ccgctcacca ttttaattgc tgtttgtgtt ccgggtaatc aggtactgcc gtttggcgat
    17341 cttgccacta tcggcttctt cgtggcgatg gcggtcgcgg tgcatcgtgg aaatctgttc
    17401 cgcaccttaa tctcgggtgt catcattatg agcatcaccc tgtggatcgc gacgcaaact
    17461 attggtttgc acacccaacc ggcggctaat gctggggcgt taaaagccgg gggtatggtg
    17521 gcttcaatgg atcagggcgg ttctcccatt acctggttac tgattcaggt tttctccccg
    17581 caaaatattc ccggtttcat tattatcggc gcaatttatc tgaccggtat tttcatgacc
    17641 tggcgtagag cgcgtggttt tactaaacaa gagaaagccg ttctcgcaga ataattttta
    17701 cctgaggggg aggttatcc
//
LOCUS       DAJTNP010000080        17362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTNP010000080 DAJTNP010000000
VERSION     DAJTNP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..485)
                     /gene="chbR"
                     /locus_tag="OO160_002921"
     CDS             complement(<1..485)
                     /gene="chbR"
                     /locus_tag="OO160_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5524964.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YR"
     gene            complement(493..843)
                     /gene="chbA"
                     /locus_tag="OO160_002922"
     CDS             complement(493..843)
                     /gene="chbA"
                     /locus_tag="OO160_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5524965.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(902..2250)
                     /gene="chbC"
                     /locus_tag="OO160_002923"
                     /pseudo
     CDS             complement(902..2250)
                     /gene="chbC"
                     /locus_tag="OO160_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2335..2655)
                     /gene="chbB"
                     /locus_tag="OO160_002924"
     CDS             complement(2335..2655)
                     /gene="chbB"
                     /locus_tag="OO160_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5524966.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2954..3292)
                     /gene="osmE"
                     /locus_tag="OO160_002925"
     CDS             complement(2954..3292)
                     /gene="osmE"
                     /locus_tag="OO160_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5524967.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3494..4321
                     /gene="nadE"
                     /locus_tag="OO160_002926"
     CDS             3494..4321
                     /gene="nadE"
                     /locus_tag="OO160_002926"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5524968.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4551..5438
                     /gene="cho"
                     /locus_tag="OO160_002927"
     CDS             4551..5438
                     /gene="cho"
                     /locus_tag="OO160_002927"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5524969.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5398..5973)
                     /gene="ves"
                     /locus_tag="OO160_002928"
     CDS             complement(5398..5973)
                     /gene="ves"
                     /locus_tag="OO160_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5524970.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6176..6661)
                     /gene="spy"
                     /locus_tag="OO160_002929"
     CDS             complement(6176..6661)
                     /gene="spy"
                     /locus_tag="OO160_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5524971.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6991..7959)
                     /gene="astE"
                     /locus_tag="OO160_002930"
     CDS             complement(6991..7959)
                     /gene="astE"
                     /locus_tag="OO160_002930"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5524972.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7952..9285)
                     /gene="astB"
                     /locus_tag="OO160_002931"
                     /pseudo
     CDS             complement(7952..9285)
                     /gene="astB"
                     /locus_tag="OO160_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9282..10760)
                     /gene="astD"
                     /locus_tag="OO160_002932"
     CDS             complement(9282..10760)
                     /gene="astD"
                     /locus_tag="OO160_002932"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5524973.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10757..11791)
                     /gene="astA"
                     /locus_tag="OO160_002933"
     CDS             complement(10757..11791)
                     /gene="astA"
                     /locus_tag="OO160_002933"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5524974.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11788..13008)
                     /gene="astC"
                     /locus_tag="OO160_002934"
                     /pseudo
     CDS             complement(11788..13008)
                     /gene="astC"
                     /locus_tag="OO160_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13454..14260
                     /gene="xthA"
                     /locus_tag="OO160_002935"
     CDS             13454..14260
                     /gene="xthA"
                     /locus_tag="OO160_002935"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5524975.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14427..15137
                     /locus_tag="OO160_002936"
     CDS             14427..15137
                     /locus_tag="OO160_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5524976.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15142..15819
                     /gene="ydjY"
                     /locus_tag="OO160_002937"
     CDS             15142..15819
                     /gene="ydjY"
                     /locus_tag="OO160_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5524977.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15834..16541
                     /locus_tag="OO160_002938"
     CDS             15834..16541
                     /locus_tag="OO160_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5524978.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16541..17089
                     /locus_tag="OO160_002939"
     CDS             16541..17089
                     /locus_tag="OO160_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5524979.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17099..>17362
                     /locus_tag="OO160_002940"
     CDS             17099..>17362
                     /locus_tag="OO160_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5524980.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGR"
BASE COUNT         4297 a         4613 c         4252 g         4200 t
ORIGIN      
        1 cggtaatgac gtaaacggtt aatgacataa aaagtaacca tctcaacaaa ctcttccagc
       61 cctgtttcgc ggaaatttaa tgaagaaatc actgtttcca cataggtgag aaaagcgtta
      121 ttggtccggt atacctgcga agcaacaaag caataaggca acaacggcag gtaatgctgc
      181 tcaaaaaagc gtttactgat cccaacgttc aatatgcgcg tggcaccaaa ctcataaaaa
      241 ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc cccgttccag taacacgcgc
      301 ttaccgttaa tctcctggaa ataacgcccg gttaatacca gagtaaattc ataatagtcg
      361 tgctgatgca gtccgctgat actctcagtt ttgttataga taaacacatg gaagtttttg
      421 ccattaaaca actgctgttc tcgggcagtg gcaatttccg gcgcgttaat cactggctgc
      481 atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag taatcagttc
      541 acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca gactcacttt
      601 catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat gcgcttcatt
      661 caatgccatc cgtgactgat ccatcatggc tttcgctgcg gcaaaatcgc cctgcttcgc
      721 ctgtttcaat gcggcatacg ccaggctacg cgcttgtcca gagttgatga taagccccat
      781 caccacttct tccagctctt cagcttctgt ttgcgtatca ggaatattat cgagatccat
      841 catacatcgt tcctctctct tcttaccggc aagattaccc gtaccggcat cgattaaaat
      901 ttcaggggtt agcgatatct tcttcgctct cttctttatc aatcgcattt tgtgctttgt
      961 tagccaccac aacaaagggc agataaatta acgttgcgat gccaaggttg aagagtgcga
     1021 ccagcaatgc ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg gttggcatgg
     1081 tccacggtgc aatattggtc accggaggaa tgatgcccat gtagtacgct gcgagggtga
     1141 ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg ttcatgataa
     1201 ttggcagacc aaacagaatc ggttcattaa tctggaagat gccggacggc agcgccagtt
     1261 ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg atcaggccta
     1321 aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg gcccagatat
     1381 ggaaggtctt accggctgcc agcgccgctt cgacggaacc atattgctga taggtcgcga
     1441 tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc
     1501 catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag cccaccacgc
     1561 tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg aagttggtgc
     1621 cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata aagccgggaa
     1681 ttaatgccga gaaagaacgc gatactgaag caggtacgct gtcgggcagt ttaatgaccc
     1741 aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata ataatcccgg
     1801 agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca ccgacactat
     1861 atggggtgac ggtcataaat gctgcaacgg ataacaaccc agccgccagc gcatcgactt
     1921 tacgctcttc tgccagcgcc atgccaataa agaacggtgc cattaaagac attattccta
     1981 atgttccgtt atatacgttg ccgccaatac ctttcagacc attaagtgtt tcaatggttg
     2041 aggcgtcgag gcgaatacct aaggaataaa aaacgacccc tccccaaagc ttagaaaaac
     2101 gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc gaataaagcc
     2161 atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag ggaggagtac
     2221 cttttcaagc gatgcaataa cattactcat agaaaaatac ccttaaaaac cgcaatttaa
     2281 atattacggt attgatttat gaaataactc tttgacggga aaatttaaaa taaattaatt
     2341 tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac catcgacttt
     2401 gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac gctggatttc
     2461 gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct gacctttttc
     2521 accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt tttctgcctg
     2581 tgcgcgcatt tttgatacca gtaaagagat agacatgccc gcagaacaaa acagataaat
     2641 gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag acactccgca
     2701 agccttgacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact ttttttgtgg
     2761 ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt ataaattgtt
     2821 tctccttgac ctgtggttat gaccctcttc acatttcggg caaataattc gcgaagataa
     2881 ttaagaactc aggccaaaaa aaaccggcgc aatggccggt ttccgttgtt actcaagctt
     2941 tcagacgaat tgattacttc gcagcctgtg gatcagtgtc gtattcagca caggtctgat
     3001 aaccggagtt gatgacatgt ccggtatcat ctaacgcgac aaagtaggtt tctgctttac
     3061 catcacgttg acccaggatg taggtctggc aagtaccgcg agcatggatc atgctcactt
     3121 cagacgaagg tttacccgca atttgtgcaa cctgcgcccg gctcatgcct tttttgacgt
     3181 ctttcaccac aggctgtaca aactggtctt tggtacgatc ataagctgta caacccgcca
     3241 gcatggttaa taccgccgct gcactcagaa ttcctgccat attcttgttc atattccgtc
     3301 ctcttgttta tcagcgtgtt agataagcct ggaatacatt gggcgctttt tcaagcccgt
     3361 gaacgaaacg gctccgcttt cagaggattc ctgtatgacg ttttaaccac cattcagccc
     3421 gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa ggaagttttg tcttttctgt
     3481 ctggaggggt tcaatgacat tgcaacaaca aataataaag gcgctgggcg caaaaccgca
     3541 gattaatgct gaagaggaaa ttcgtcgtag tgtcgatttt ctgaaaagct acctgcaaac
     3601 ttatccgttc attaaatcac tggtgctcgg gatcagcggc ggtcaggact ccacgcttgc
     3661 cggaaagctg tgccagatgg cgattaatga gctgcgccag gaaactcgca acgaatcact
     3721 gcaatttatt gccgtacgcc tgccctatgg tgttcaggct gacgaacaag attgccagga
     3781 tgccattgcc tttattcaac cggatcgcgt attaaccgtt aatatcaagg gcgcggtatt
     3841 ggctagcgag caggcattgc gggaagcagg cattgaactg agcgattttg tccgtggcaa
     3901 tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt gcgggtatga ccagcggtgt
     3961 cgtggtgggc accgatcatg cagcagaagc cattaccgga ttcttcacta aatatggtga
     4021 cggcggtacg gatattaatc cgctgtatcg tctcaacaaa cgccagggta aacagttact
     4081 gacggcatta ggttgcccgg aacaccttta taagaaagcc ccaactgccg atcttgaaga
     4141 tgatcgccct tctctgccag atgaagtggc actcggcgtg acctatgaca atatcgacga
     4201 ctatctggaa gggaaaaacc tacctgaaca ggtcgccaga acaatagaga actggtatct
     4261 gaaaaccgaa cataaacgcc gtccgccaat taacgttttc gatgatttct ggaaaaagta
     4321 ataatttgca cctattggat tgtgcgaaaa agagtaattt gttcacgccg gatgcggcgt
     4381 gaacgcctta ttctacctac aaaactatgc aaattcaata cattgcagga gtcgaatagg
     4441 cctgacaggc gtagcacgtc agacggtgta acctttgtca tcaacccgcc tcttttttaa
     4501 tcgcctcccg cctgttacac tggatagata accagcattc ggagtcaaca gtggtacggc
     4561 gtttaacttc tccgcggctc gaatttgaag ctgcggcaat ttatgaatat cccgaacatt
     4621 tacattcatt ccttaatgac ttacccaccc gaccaggggt gtatctgttt catggcgaaa
     4681 gtgacaccat gccgctctat atcggcaaaa gcgttaatat ccgcagtcgg gtgctttctc
     4741 atttacgcac cccggatgaa gccgccatgc tacggcaatc ccggcgaatt agctggatct
     4801 gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg attaatcaaa gaacaacagc
     4861 cgctgtttaa taaacggttg cgccgcaatc gccagctctg tgccctgcaa ttaaatgaaa
     4921 agcgcgtcga tgtggtgtat gccaaagagg tggatttttc acgagccccc aacctgtttg
     4981 gcctgtttgc caataggcgc gcagctttgc aagcattgca gagcatcgct gatgaacaaa
     5041 aactttgtta tggcctgttg ggactggaac cgttaagtcg cggtcgtgca tgttttcgtt
     5101 cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga gagccatgag gaacatgcgc
     5161 tacgcttgcg ccagtctctg gagcgtttgc gagtggtgtg ttggccttgg caaggtgcgg
     5221 tggcactgaa agaacagcac ccggaaatga ctcaatatca tattattcaa aactggctgt
     5281 ggctgggggc ggttaattcg ctggaagaag cgacaacgtt aattcggaca cccgccgggt
     5341 ttgatcacga cggttataaa attctttgta agccgctgct ttccggtaac tatgaaatta
     5401 ctgaacttga tccggcgaat gaccagcgag ccagttgatt tcactgaaca acagtttacc
     5461 ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac cagcacacgc cctggtcggt
     5521 agtgagcagt ttgtcgccca gttgccacgc cccgttaatc acaaacacca ccccgccgcg
     5581 tgagccaaag gtagtaaacg tacgttcggc aattctgact ttcgccttac agacatccag
     5641 gcgcgtcata atattgaaat ccatcgacat ctgccctgcc gtcagtttcg cttttaccac
     5701 ctggtccgcc gtaaaggcaa aaggctgtaa cggttttaag gtatggttaa agcggtctgc
     5761 gctttcaagg agcatctcgc cgccttccag caacgtcact atcctttcca tgccgggaaa
     5821 caaagaaaac tccccattcg ccgcgatgga tgcaatgctg gcacgccagt aaaaatcacg
     5881 ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg gcagcgtttc gccacagatt
     5941 caccgacatt ttacgcatat caaagtattc catactcgct cccggcccgt tcttttcatt
     6001 gttatcgttc aggtaaccgt ggatggttat cggcaactcg ggtgatttac gttagtggtg
     6061 atcaggcttt ttttacaact gtttgagaag agaaaagaaa accgccgatc ctgtccaccg
     6121 cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg aaagattatt
     6181 cagcagttgc aggcatttta ccttttgccg ctggacgttc tgtcagacgc ttctcaaaat
     6241 tagcattaaa ttgctttttc tgttccggcg tcaggatgtt gtaaattttg ttctgggttt
     6301 ccatgtgcgc cagcatgtta gctttgcgct gttcttccat ttttgcgatc tgcgcttcag
     6361 cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg cattgcgcgg cgttcttcca
     6421 gcggcggacg tttcatctgg tcacgctggc ctttcatgat ttcgtggatc tgctgtttct
     6481 gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg atgcggaccg aacttgcctt
     6541 tgtggtgcat catcggcttc gcgtcagccg gtgctgcggt agtggtgtct gcggcatggg
     6601 ccaggttagc cgcgccaaga gccagggtag aggcaacaaa cagtgcagtt aatttacgca
     6661 tattctatat ccttcctttc agttatttat tacggctttc ttaagtagcg tgccgtgttg
     6721 acgagattaa ctttactgac tttagcgtca attaatcaga gcaacggtaa aacaatgaaa
     6781 gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag aaaatttgat ggagagtgat
     6841 gagagaatat tacaacacga tgattttgca gagattatga agaactatac cggatgactg
     6901 gtgataaata aagcaaataa ccaggattaa tctgtattaa tttataagaa agcaacttaa
     6961 tacccgcaga atgatttctg cgggtaagta ttagcttatt ttttcgagca ttaatcccgc
     7021 gcgtaatccc aacgctacca acggattagg gaataacaca tactctacat catgggttac
     7081 ggtaaaacgt tcctctccgt cctgcgccag caatgttcct ttctcaaacg gcataaaatt
     7141 cagcgtgtca cttgccatat gcatttcgaa ggacggcgag tggcgagtaa tttgcgaaac
     7201 cacccgataa cggagcggcg gtgttctcac gataccgaca ctctcaccag atagcagcgc
     7261 agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg ttttgcccaa agggcaacgc
     7321 tttgccaagt tccaacgtac aggccagcgc gccaaaatgt ctgacgctga aatgggtaaa
     7381 cgtaccacca ggctcctgat ggaacaccag cgcctccagc cccgccgcac ccagccacgt
     7441 cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat acaccgaact gcggatgcaa
     7501 ggagccacga attgcggtat gtagatcaag gtgccaacgc acagattctt tgccctggtc
     7561 ataaaaatct tccaggcact gttccagttc gcgcgcccga caggtttctc cgctttcagc
     7621 aaatagctgc caacgaccgc caaacattcg gttcatatcg ctatggcaat aacgtttccc
     7681 ttgcttcagc gcaggaggat tcccgaggat caccagcaac cgccaacgta acgggatctc
     7741 gccgtgagat atcgcgccaa gcaacgcgtc cagcatttcc acaggtgccg tctcattacc
     7801 gtggatcccc gcagaaatca ccagcgcgcc ttgcggtggc gttaatggcg tcagttccag
     7861 cacaccatcg cccagccagc gccagcgaac gccgttgatt tctcgctcgg tgataaccgg
     7921 ttttttaccc gttaaggtca gagcaagaaa attatccatt gccccctccc tcgcgctgga
     7981 acggataaac cgaaccgaga ttcagtaatt ggctcaatac atccagtgct tcccgccctt
     8041 cgcgcagcaa ttgcgggtcg gccagatcgg cagcagtaag acgatcgcgg tagtaacgat
     8101 ccacccagtc attgagcgca ttaaacaagg tatcgttcat catcaccgcc ggattcaccg
     8161 cccggcgttc ttcttctgtc aataccaccc gcaaccgcag gcacgccggg ccgccgccat
     8221 tcgccatgct ttcacgtaag tcaaagactt ttagttcgct aatcgggttg tcagcggcaa
     8281 ggagttcatt gagataaccc catactccgg cgtgttcccg acactcctga ggcagcacca
     8341 acatcatgga accatcatcg cggctcaaca gttggctgtt aaacagatag gtagacaccg
     8401 catccgacac ggaaacctga gttgccggaa cttctatcgc cataaaaccg ttcacccgcg
     8461 tgccagtaac tgtgactggc gagcgaacgc ctgttggtgg caaaacagca cctggcggtt
     8521 actcacggca atcacgtcat tatgaaaaac gctctggtcg ataacgtccg ggttttgctg
     8581 ggcgaaaatc acctgttggg gattcacctg attaagcctt gccaccgcct cgctggcttc
     8641 gcgagtctgt cgcgccggat aacgggaagg ccgggtatca ttgccttctt ctcgcccgta
     8701 gacaaaaagt agcatacccg gttcaccgta atgaccgccg agacgattgt ggtttgccgc
     8761 cccctcatca ccgagcaacg ctacctgcgg caacgccgaa tggacgctaa atttctcttc
     8821 gtccttaaaa atcgctttta acagcgattc agtgacgggc gcttccagcg aacggtgaaa
     8881 tttattgttc aggttggcaa cggtgagatg cactttgcca tccagcgtat cggcagatgg
     8941 cgcgatcgtt gccgcattgg ctacccacat tggcgaagcg gaactgacgc tggaaagcca
     9001 gtgcggtgcc tggcgtgcaa ctttttccag tacctgctca tcgctaccac tgaatcccaa
     9061 ctgacgcagc accggaataa acggacgctc gtgcggcgga atcacggcct gggggaatcc
     9121 cgcatcggca agggctttca ttttcagtaa gccctgtttt gccgccagtc gcgggttaga
     9181 cacctgaaaa cggtgacggg tagaggcttc attaccaaac gacaggcccg cgtaatgatg
     9241 cgtcagccct accagcccgt cgaaattaac ttcccaggcg ttcatcgcac cacctcatcg
     9301 gaaaaatcca gcccggggtt aagcgtggcg ggcaatgtta acgagtccga ctccaggctc
     9361 gccataggcc atgcgcagta atctgcggca taccaggcgc tggggcgatg gttaccggaa
     9421 gcaccaatgc cgccgaatgg ggcggtactg gcagcaccag taagcggttt gttccagtta
     9481 acaatccccg cccgcgcctc cagcaacagt tgatcgaatt tttcccgctc gggggaaacc
     9541 agaccgcaaa agagtccgaa gcgagtgttg ttcgccatcc gaatcgcctc atcaaaagta
     9601 tcataacgcc agacgcgcaa aaacggcccg aacacctctt catccggcac accgccaacg
     9661 cccgtcattt caatgatccc tggtgtcagc aacgatgtcc ctgcttgtaa taagcgcggc
     9721 gcaagcaggg ttcgtccgcc catcgcttcc agttgctgcc aggcagtaac cacctgctgt
     9781 gcggcctgtt cagaaatcag cccgccaata aacggctgcg gttcgtcatc ccagttgccc
     9841 ggcgttaatc gctggctgac ggcaaccaga ctagcaagaa acgcatcgcc ctgcgctccg
     9901 cttttgagca ataaacggcg ggcgcaggtg cagcgttgcc cggctgtgac aaacgccgac
     9961 tgaatggtca gatggacagc cgcgtcgata tccgccacct catcgataat tagcggatta
    10021 ttaccgccca tctcaagggc gagaattttc tccggctgac cggagagctg gcgatgcagc
    10081 tggtagccgg tattggcgct accggtaaac agcaaaccgt cgagatcctc cagcgcactc
    10141 agcgcctgac ccgtttcacg cccgccctgc accaggttca gcacgcccgg cggcaagcca
    10201 gcctgctgcc ataaacgcat taccgcttcg ccactccacg gtgtcagttc gctgggttta
    10261 aagataatgg tgttacctgc cagcaatgcc ggaacgatat gtccgttcgg caaatgacca
    10321 gggaaattat acggcccaaa caccgccagc acgccgtgcg ggcgatgtcg caggctcgcc
    10381 gcaccgtccg gcatttcact acgctgctcg ccggtacgaa cgtgatacgc cttaattgat
    10441 atcgcgattt tattgatcat cgccgtcact tcggttgccg cttcccagcg cggcttaccc
    10501 gtttctctgg caataatcgc ggttaattcg gctttattgc tttccagcaa tccggcaaag
    10561 cgttcgacaa cgacctgacg ttcagccaat gagagccgcg cccagcgcgg aaacgccgca
    10621 cgggctgccc gacaagcctg ttcgacctga gcggcatcgg catcattgcc ttgccataac
    10681 acctcgcccg ataccggatt acgcttcaca cgcgatgcgc cctggcccgt tatccagtca
    10741 ccgttaatcc ataaagtcat gctgttttct cctctgcgca caggcgcacc agacgaacgc
    10801 gatcccctgc gtggcatttg agggcatcca gttgtgcggc ggttaaaatc aaacgctcgg
    10861 ttgccggatc ggtacgcgcc agcaccacgc ggaaatggtg ataattttca ttggcgacca
    10921 ggcaggccgg aaaatcgccc tgcgcaggct gcccttctgc cacttccacc agccgacttt
    10981 tacggatggc gcgcacgcgg tcgatgtcac actcaagcgt tggcccgccg tcgaagatgt
    11041 cgatatagtt gcggtaacga aaaccttctt tctccagcac cgcgcgggca ggcgcggttt
    11101 gcggatgtac ctgaccgatg acgtcctggg cttcctggga taaaaagtgg gtatagatcg
    11161 gatgtttcgg catcagttct gcaataaatg ccttttgccc ggtgccgcag agaaaatcgg
    11221 cgcggctaaa atccatcgaa aagaagcgtt taccgaggct ttgccagaac ggtgaatagc
    11281 cgtgttcgtc aatcaccccg cgcatttcag caaccacttt gtcattaaac ttgtcgcgaa
    11341 aagccgccat aaacataaag cgcgatttcg atagcaaata gccgttgccc tctttgcgcc
    11401 agtccgggtc gagaaacagc gtgcacagct cgctgctgcc ggtgtgatcg ttactaagaa
    11461 acagcgtcgg caatgcgtta tagacattca gctcttttga ggcgtgaacc aacgtgccga
    11521 cgcgatagtt gtaccaggga tcgttcagcc caaccgccac ctcaatagcg caaatccccg
    11581 ccacggtgcc tgtctcgcta tcttccagca cgaacacata gccctgctcg cttttgggca
    11641 gttcaccttg ccaggttttg attgcccttt cgatacgcgc cgaaagcgtg gcttcattgg
    11701 cgggaagcga cgtcaggccg ccgcccgttt tgctggcaag ctgcatcagc gccgagacat
    11761 ctgagcgctc aacgggacgg atgaccatca tgatgaacct cggctaacaa agtgttcgca
    11821 agcagctgca aagcgatcca gtccggtcgt cacctcttct tcgctgacat tgagcgcagg
    11881 cgcaaaacgc accacgttgc cacccgcaat cagtaccatc acgcctgctt tcgccgcttc
    11941 cagagagatc tgtttcgctt gcccggcgta atcggcattc agtacacagc caatcaacaa
    12001 acctaagccg cgaacttcac tgaacaaacc atagcggtga ttaatagtat taagacgctc
    12061 aacaaaccag tcgtgacgct gtttaacgcc attaagcatc tctggtgtgt tgatgagctc
    12121 cagcactttg cctgctaccg ccgaggccag cggattaccg ccataggtgg tgccatgagt
    12181 gccaacggtc atcacgcggg cgcactcttc ggttgccaac aacgcaccga ccgggaaacc
    12241 gccgcccagc gctttggcgg tggttaacag atcgggcgtc acgccgtagt gcatataggc
    12301 atacagctca ccagtgcgcc cgacgccggt ttgtacctca tcaaaaatca gcaaggcatt
    12361 gtggcggtca cacaattcac gcagaccttg taaaaacgcg ttgctggctg gcaccacacc
    12421 gccttcgccc tagatgggct cgacaatcac cgcacaggtg gcgtcgtcaa tcagcgcgct
    12481 ggcagagtta atatcgttat atgcagcatg acgaatatcc gccggcagtg gcgcaaaatc
    12541 ctgtgaatag gctggctgcc cacccgcact gacagtaaac agcgtgcgac catgaaacgc
    12601 atttttgaac gccacgatgc cactcttatg gctgccgtag cggtcgtgag cgaatttacg
    12661 cgccagtttt agcgccgctt cgttggcttc cgcaccggag ttacaaaaga agacgcgatc
    12721 ggcaaacgtg gcgtcgatca attttttcgc cagtcgcagt accggctcgt tggtgtaacc
    12781 gttgccggta tgccagaact tactcgcctg ttcgttcagc gcttcacgca gttccggatg
    12841 cgcatggccc agcgcgttca ccgcaatgcc acccgcgaag tcgatatact ctttcccctg
    12901 ctgatcccac aagcgcgaac cttcgccacg taccggtata aagggtgccg gagcgtaaac
    12961 aggtatcatc cattcatcaa agttttcacg cgtaattggc tgagacatag cgacctctac
    13021 agtaaataat tcgttatttg tatgttaaca ataagtaatg tttgcgctgt aaatgtagat
    13081 tgcagggttc gtgccagcca gtgataaaag tgcataaacg gcggaggcta actggaaatc
    13141 aaggagttat aaccaaacca tatgcattta aagtgcatat aaagtgaata cgtttgcgat
    13201 gtgggtgaat aaaaagaata aaaaacgcaa tgttatgcag aagtaaaata taattctaga
    13261 attgtgatca ttgacgaaat ttactggaaa ttactgcgcc attctgacgc agcgcgcacc
    13321 aaaagcgggc attttttgcg ccatcgttga catcattaac aaccatcgat caaatcactt
    13381 aacaacaggc ggtaagcaac gcgaaattct gctaccatcc acgcactctt tatctgaata
    13441 aatggcagcg actatgaaat ttgtctcttt taatatcaac ggcctgcgcg ccagacctca
    13501 ccagcttgaa gccatcgtcg aaaagcacca accggatgtg attggcctgc aggagacaaa
    13561 agttcatgac gatatgtttc cgctcgaaga ggtggcgaag ctcggctaca acgtgtttta
    13621 tcacgggcag aaaggccatt atggcgtggc gctgctgacc aaagagacgc cgattgccgt
    13681 gcgtcgcggc tttcccggtg acgacgaaga ggcgcagcgg cggattatta tggcggaaat
    13741 cccctcactg ctgggtaatg tcaccgtgat caacggttac ttcccgcagg gtgaaagccg
    13801 cgaccatccg ataaaattcc cggcaaaagc gcagttttat cagaatctgc aaaactacct
    13861 ggaaaccgaa ctcaaacgtg ataatccggt actgattatg ggcgatatga atatcagccc
    13921 tacagatctg gatatcggca ttggcgaaga aaaccgtaag cgctggctgc gtaccggtaa
    13981 atgctctttc ctgccggaag agcgcgaatg gatggacagg ctgatgagct ggggggtggt
    14041 cgataccttc cgccatgcta atccgcaaac agcagatcgt ttctcatggt ttgattaccg
    14101 ctcaaaaggt tttgacgata accgtggtct gcgcatcgac ctgctgctcg ccagccaacc
    14161 gctggcagaa tgttgcgtag aaaccggcat cgactatgaa attcgcagca tggaaaaacc
    14221 gtccgatcac gcccccgtct gggcgacctt ccgccgctaa tttagcagct ctcctggctc
    14281 aaactgggtc aggagaatta accttgagaa aaatcaacaa actgtcagta atgatttgtt
    14341 gcctgccgtc ctttgttata ccgtcactgc gtttttagtt gtctgaccac ttctctatta
    14401 tcaagtttga tataggaaac tccacgatga acgctgagcg taactttctg tttgcctgtc
    14461 ttatttttgc gctggtcatt tacgctatcc acgctttcgg tttattcgat ctgctcaccg
    14521 atttacccca cttacagaca ctcattcgcc agagcggatt gttcggttat agcctctata
    14581 ttctgttatt catcaatgcc cccctctttc tgttaccagg aagcatactg gtgatcgctg
    14641 gaggaatagt ttttggcccg ctcttaggga cacaactctc attaattgcc gcaacgctgg
    14701 cctcctcgtg ctcattcctg ctggcgcgct ggctgggacg tgatttactg ctgaaatacg
    14761 tgggccatag ccataccttc caggccattg aaaaaggcat tgcgcgtaac ggtattgatt
    14821 ttcttattct gacccgctta atcccgttgt ttccttacaa tattcaaaat tacgcttacg
    14881 gattcaccac catcgccttc tggccttata cccttatttc ggcactcacg accctgcccg
    14941 gtattgttat ttataccgtg atggcaagcg atctcgccaa tgaaggcatt acgctgcgct
    15001 ttattttaca actctgtctg gcgggcctgg cgctgtttat tctcatccag ctcgcaaaac
    15061 tctacgcccg acacaaacat gtggatctgt ctgcttcgcg ccgcagccca cttactcacc
    15121 caaaaaatga aggatagaac gatgtcgcaa cattattcag tgtcatggaa aaaaggactg
    15181 gctgcactct gcttactggc tgttgccggg cttagcggct gcgatcaaaa agagaatgcg
    15241 gcggcaaaag tggaatacga cggactttcg aacagccaac cgctgcgcgt cgatgccaat
    15301 aaccatacgg taaccatgct ggtgcaaatt aatggtcgtt tcctcaccga cgacactcgt
    15361 cacggtattg tgtttaaaga tggctccaac ggacataaat cgctgtttat ggcttatgcg
    15421 cccccgaaag cattttatga agccctgaaa gaggcaggtg gtacgccggg cgaaaacatg
    15481 acgatggata ataaagaaac gactcatgtc acaggcagca aactggatat ttcggtcaac
    15541 tggcaagggg cggcaaaagc gtattccttc gatgaagtga ttgttgacag taatggcaag
    15601 aaactggaca tgcgctttgg cggtaattta acggcagcag aagagaagaa aacgggttgc
    15661 ctggtgtgtc tggatagctg cccggtcggc atcgtcagca atgcaacata cacttatggt
    15721 gcggtggaaa aacacggcga agtgaaattc aaaggcaatg cctcagttct cccggcggat
    15781 aacacgctgg caacggttac ctttaaaatc accgaataaa gccaagataa aggatgatga
    15841 agatacaatc gcgtaaaatc tggtactacc gtataaccct catcatcctg ttgttcgcta
    15901 tgctgctcgc atgggcgctg cttcccggcg tccatgagtt tatcaatcgc agcgttgcgg
    15961 cgtttgccgc cgtggaccaa cagggtatag aacgctttat tcagtcttac ggcgcactgg
    16021 cggcagttgt ctcgttcttg ttgatgattt tacaggccat tgctgcaccg ctacctgcgt
    16081 ttttgatcac ctttgccaat gcgtcgctgt ttggcgcgtt ctggggcggc ttgctgtcgt
    16141 ggaccagttc gatggccggc gcggcgctgt gcttttttat cgccagagtg atgggccgcg
    16201 aagtggtgga aaaattaacc ggcaaaaccg tgcttgacag tatggacggc tttttcactc
    16261 gctacggcaa acacaccatc ctggtctgtc ggttattgcc ttttgtccct ttcgatccaa
    16321 tcagctatgc tgccggtctg acttcaatac gttttcgctc gttttttatc gccaccgggc
    16381 ttggtcaatt accggcgact attgtttatt cctgggcagg cagcatgtta acaggcggta
    16441 ctttctggtt tgtcaccgaa ctgtttattc tgtttgccct gaccgtggtg atttttatgg
    16501 cgaagaaaat atggcttgag cgccagaaga ggaatgactg atgggtttac cgccgcttag
    16561 caaaattcct ttcattttac gtccgcaggc gtggctgcat cgtcgccatt acggcgaggt
    16621 gctaagcccg attcgctggt gggggcggat cccgtttatc ttttatttgg tgtcaatgtt
    16681 tgttgggtgg ctggagcgca aacgctcacc gctcgatccg gtagtgcgat cgcttgtcag
    16741 cgcgcgcatt gcgcaaatgt gcctgtgtga gttttgcgtg gatatcacca gtatgaaagt
    16801 cgccgagcgt accggcagca ccgataaact gctggcagtg gctgactggc ggcaaagccc
    16861 gctctttagc gatgaagaac ggctggcgct ggagtacgcc gaagccgcaa gcgtcacacc
    16921 gccaacagtc gatgatgccc tgcgtacccg actggctgcg cattttgacg ctcaggcgct
    16981 caccgaactg acggcattga tcggcctgca aaacctgtcg gctcgtttta attctgccat
    17041 ggacattccc gctcaggggc tgtgccgtat tccggaaaaa cgttcttaag gagggaagat
    17101 gcgccattgt gggtggttgc tgggattgtt atcgctgttt tctttggcaa cacatgccag
    17161 tgactggcag gaaattaaaa atgaggccaa agggcaaacc gtctggttta acgcctgggg
    17221 cggcgatacc gcaattaacc gctatctcga ctgggttagc ggcgagatga aaacccatta
    17281 cgctataaac ctgaagattg ttcgtctcgc ggatgccgca gacgcggtga agcgcattca
    17341 gaccgaagct gctgccggtc gt
//
LOCUS       DAJTNP010000081        17089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTNP010000081 DAJTNP010000000
VERSION     DAJTNP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..508
                     /locus_tag="OO160_002941"
     CDS             <1..508
                     /locus_tag="OO160_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5524981.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO160_002942"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO160_002942"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5524982.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OO160_002943"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OO160_002943"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5524983.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OO160_002944"
     CDS             complement(2876..3073)
                     /locus_tag="OO160_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5524984.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO160_002945"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO160_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5524985.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO160_002946"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO160_002946"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5524986.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRKNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO160_002947"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO160_002947"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5524987.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO160_002948"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO160_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5524988.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO160_002949"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO160_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5524989.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO160_002950"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO160_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OO160_002951"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OO160_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5524990.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO160_002952"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO160_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5524991.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OO160_002953"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OO160_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5524992.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OO160_002954"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OO160_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5524993.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OO160_002955"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OO160_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5524994.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO160_002956"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO160_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3993 a         4863 c         4444 g         3789 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gatttttccg cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgccagg cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac ccataattat tttctgtaa
//
LOCUS       DAJTNP010000082        17070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTNP010000082 DAJTNP010000000
VERSION     DAJTNP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..457)
                     /gene="rlmB"
                     /locus_tag="OO160_002957"
     CDS             complement(<1..457)
                     /gene="rlmB"
                     /locus_tag="OO160_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5524995.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLI"
     gene            complement(637..3078)
                     /gene="rnr"
                     /locus_tag="OO160_002958"
     CDS             complement(637..3078)
                     /gene="rnr"
                     /locus_tag="OO160_002958"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5524996.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3117..3542)
                     /gene="nsrR"
                     /locus_tag="OO160_002959"
     CDS             complement(3117..3542)
                     /gene="nsrR"
                     /locus_tag="OO160_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5524997.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3747..5045)
                     /gene="purA"
                     /locus_tag="OO160_002960"
     CDS             complement(3747..5045)
                     /gene="purA"
                     /locus_tag="OO160_002960"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5524998.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5149..5346)
                     /locus_tag="OO160_002961"
     CDS             complement(5149..5346)
                     /locus_tag="OO160_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5524999.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5428..6432)
                     /gene="hflC"
                     /locus_tag="OO160_002962"
     CDS             complement(5428..6432)
                     /gene="hflC"
                     /locus_tag="OO160_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5525000.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6435..7694)
                     /gene="hflK"
                     /locus_tag="OO160_002963"
     CDS             complement(6435..7694)
                     /gene="hflK"
                     /locus_tag="OO160_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5525001.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7780..9060)
                     /gene="hflX"
                     /locus_tag="OO160_002964"
     CDS             complement(7780..9060)
                     /gene="hflX"
                     /locus_tag="OO160_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5525002.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9136..9444)
                     /gene="hfq"
                     /locus_tag="OO160_002965"
     CDS             complement(9136..9444)
                     /gene="hfq"
                     /locus_tag="OO160_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5525003.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9530..10480)
                     /gene="miaA"
                     /locus_tag="OO160_002966"
     CDS             complement(9530..10480)
                     /gene="miaA"
                     /locus_tag="OO160_002966"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5525004.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10473..12320)
                     /gene="mutL"
                     /locus_tag="OO160_002967"
     CDS             complement(10473..12320)
                     /gene="mutL"
                     /locus_tag="OO160_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5525005.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12330..13667)
                     /gene="amiB"
                     /locus_tag="OO160_002968"
     CDS             complement(12330..13667)
                     /gene="amiB"
                     /locus_tag="OO160_002968"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5525006.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13686..14147)
                     /gene="tsaE"
                     /locus_tag="OO160_002969"
     CDS             complement(13686..14147)
                     /gene="tsaE"
                     /locus_tag="OO160_002969"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5525007.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14119..15666)
                     /gene="nnr"
                     /locus_tag="OO160_002970"
     CDS             complement(14119..15666)
                     /gene="nnr"
                     /locus_tag="OO160_002970"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5525008.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15665..16804
                     /gene="queG"
                     /locus_tag="OO160_002971"
     CDS             15665..16804
                     /gene="queG"
                     /locus_tag="OO160_002971"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5525009.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16787..16840)
                     /locus_tag="OO160_002972"
     CDS             complement(16787..16840)
                     /locus_tag="OO160_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525010.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3866 a         4863 c         4296 g         4045 t
ORIGIN      
        1 gaatcagcgg aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct
       61 gtgcggagcg atctttcggc acaatcaccg catgaacgcc tgcggcgtcc gcactacgca
      121 ggcacgcgcc gaggttgtgc ggatcggtta cgccgtcgag gatcagcagg aacggttgat
      181 cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc
      241 gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg
      301 ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat
      361 cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg
      421 cctgcactgc gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt
      481 tgcctgatgc actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg
      541 attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc
      601 agcaatctaa ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc
      661 cgcacgcttc gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc
      721 ttttctcgcg tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct
      781 gtctggctca aagtttacct ttttaccgac ctgacaacgc ttgccgcctt ttttgcctgc
      841 atcgcctttt ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact
      901 ggagatcaga ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc
      961 cacgcgatcg cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac
     1021 ctggtcaaag cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa
     1081 caagtcgtcc agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa
     1141 gacgttacct acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt
     1201 tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc
     1261 catcgaataa tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc
     1321 cagcagatat ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt
     1381 aaagtgcgca taggactgca atgccagacc gaagtgacca cggttttctg gatcgtaaat
     1441 cgcctgtttc atcgagcgta gcagcatggt ttgcagcatt tctgcatcag gacggtcggc
     1501 aaccgactcc agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc
     1561 cagccccagc tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc
     1621 gtgaatacgg aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt
     1681 cgccagaatc atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat
     1741 acgttcaata cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat
     1801 cccaccgcgt tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag
     1861 atgcttaacc agcggggcgt actgctcgcg cagatcctga tcgccctgca gaatatgcca
     1921 gactttggtg taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc
     1981 cgtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg
     2041 cgggttgagc gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga
     2101 agggaagtac accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg
     2161 cacatagtag ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt
     2221 ctcgcagtaa actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg
     2281 taaatcgcgc aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc
     2341 cgcaacctgt tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc
     2401 gatatcaacc gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac
     2461 cgctttggtg cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg
     2521 cgcgcccatg atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg
     2581 aaccacaaag ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg
     2641 caccagtacg cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg
     2701 cgccagcacc tgatcgccat gaatgcaggt tttcatctgc tcgctggaga gatacaaatc
     2761 atctttacgc ccttcaaccc gcagaaagcc gtagccatca cggtgaccaa taacggtacc
     2821 tttcaccagg tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg
     2881 accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc
     2941 aatgtgcagt tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg
     3001 ttcgaggata aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg
     3061 gaaaggatct tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac
     3121 tccaccagca ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag
     3181 ttatccagtt ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag
     3241 gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg
     3301 cgcaccacat caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt
     3361 tttccacgaa cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata
     3421 tgattacggg agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct
     3481 ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc
     3541 acatcaacct caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca
     3601 caagctgtgt cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg
     3661 tgtcggatag cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg
     3721 caaaatatct gccaggcgta ccagaattac gcgtcgaacg ggtcgcgcag aatcatggtt
     3781 tcagtacgat ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata
     3841 cgcttgatgt agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg
     3901 gattcagacc agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct
     3961 gccagcggag tggtagtcac ttcgcgacca tccggcatac ggtaagccac gcagagttta
     4021 acctctttca ggccatccag aacgtccagt ttagtcaggc agaagccaga cagggagttc
     4081 agctgtaccg cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc
     4141 gtagttgcac cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt
     4201 tcggccggga aaggacctgc acctacacga gttgagtaag ctttgaggat acccagaacg
     4261 taatcaacat aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg
     4321 gaagaagtta cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct
     4381 tcgaacatga cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc
     4441 accatagaag tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca
     4501 accgcttcag ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc
     4561 agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgcc
     4621 actttatctt cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca
     4681 cgcgctttct cacgcgcgtt atccagagca acgtgataat caaggatcag cggacatgct
     4741 tcagacagca gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct
     4801 ttcatcagcg cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg
     4861 cggagaatac ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta
     4921 tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga
     4981 tcgacgatct tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta
     5041 cccatttttc aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt
     5101 acagcacttt ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt
     5161 tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg
     5221 taaaatatta tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta
     5281 aagcatcggc cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga
     5341 attcattcat tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct
     5401 ttattgacct gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta
     5461 gcggaagaaa tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt
     5521 ctcataagca cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc
     5581 agcaaacagt ttggctgctt cggcatcgcc ttcaccacgc ataatgcggc cctgacgctc
     5641 agcttctgcc agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc
     5701 ttcctgacct tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt
     5761 gtagatcgct tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac
     5821 ttcaataccc agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc
     5881 agccgttacg cgctctgccg cttcggcaat ggcgttatct gctgccgggg tagtaacttc
     5941 atcttctgta cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc
     6001 acgggaatcg gtgacgatat ctttcacgtc caggcgacca atttcagaac gcagacggtc
     6061 agagaactta cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag
     6121 gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa gagtcgacga tcaggtcttt
     6181 cttctctttg gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag
     6241 cattttcacc gtttcaatga acggtatctt gaaatgcaga ccaggctcat aaaccaaagg
     6301 tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc
     6361 tttgacgaca aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac
     6421 tgacttacgc atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg
     6481 cgttggtcca taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg
     6541 gaggaagagg ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca
     6601 ggggcgttac cacctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta
     6661 tcgttaacca gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga
     6721 cgctcgcgag taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc
     6781 acttcaccct gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata
     6841 cgttgcgcct gaccgttcgc acgcggctga acttcgttgg aatacgcttc tgcttcacga
     6901 atgtattgct gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct
     6961 tccggcggac gagcagcctg gaagttgacg tccagcagcg tgatacccat gtcatacgga
     7021 cgaatcgtct cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc
     7081 agaatgcggt ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga
     7141 cgcaggctgt catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg
     7201 tactgcacgt tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa
     7261 gcggccagtt cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt
     7321 ttccagttca gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg
     7381 cgttcggctt ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc
     7441 gctgcgatgg taacgacacg accgccaagc tgcgggcgcg ggccttgtga tgaactgcca
     7501 ccgccagatc cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg
     7561 aagatatcat ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca
     7621 gagttgccgc caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc
     7681 tgattccacg ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga
     7741 cgccagagga tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa
     7801 cgccggttct tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact
     7861 tacgctgccg tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact
     7921 cctcagacgc ccttcctgcg gtggcagacg caatgtatgc tgcgccacct cgccggaaag
     7981 ccgctccgtc aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca
     8041 gacacggatc ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat
     8101 atcgatcttg ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac
     8161 cgtattcacc gcttcgatgt tttcttgtac acgcacatcc gccgcatcaa tgacgtgcag
     8221 cagtaatgtg gcttgccgcg tctcttgtaa cgtggcttta aatgccgcca ccagatcgtg
     8281 cggcaggtgg cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catctgcaac
     8341 gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg
     8401 cgcttcggtg atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga
     8461 aacagtagga acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac
     8521 tctttccagg cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc
     8581 gagctgggtt tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt
     8641 ccagccacgc accaggcgcg tagccagatg gcgcagctgc gccagctcaa cctgcaactt
     8701 accctcatgg gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat
     8761 aacacgacac tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc
     8821 aaaaagaacg accgaagcac ccatcgcttt gacagcttcc gcaatttcaa ctgctttacc
     8881 ttcacctaca aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc
     8941 ttcgacaccg gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc
     9001 ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa
     9061 gcgtataacc ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa
     9121 aaacagcccg aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc
     9181 gctgctacca tgatggtagt tactgctggt accgccaccg gcgttgttac tgtgatgaga
     9241 aaccgggcga gatgggacaa cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt
     9301 gtttttcaac aggatcacga actgatcaaa agactcgatt tgcccttgca gcttaatacc
     9361 attcaccaaa taaatagaaa ctggaacacg ttcccgacgc agtgcgttca ggaacggatc
     9421 ttgtaaagat tgccccttag ccattctctc ttttccttat atgcttattt gtactttgaa
     9481 cctttcgatt ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt cagcctgcga
     9541 tagcaccaac aacctgtaat acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc
     9601 agtgaacccc ttcccaacca cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg
     9661 cgcaaacacc tcgataaacc atttcatcgt atgagatttc gccttcaagg taagaccaca
     9721 tctggcgata acccacgcaa cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa
     9781 aaagcgcccg gacttctgct tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa
     9841 tgcgttgatg gagcagttca cggctcgccg gggcgatggc gaactgatgc acctgatacg
     9901 gtagagcgtc tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa
     9961 cttccagtgc ccgggaaagc ctttgtggat catttggatg aatccttgct gccgcaaccg
    10021 gatctacctc ctgaagttga cgatgcaatg actcccaacc ttgctctgcc gcctgttgct
    10081 caattctggc ccgcacttcc gggtctgccg acggtagcgg cgacaaccct tccagcaatg
    10141 ccttgaaata caacatcgta ccgcccacta acagtgggat ccgccccgcc gcggtgatat
    10201 cggccatttc cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat
    10261 cgcgaatatc cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg
    10321 tcccgatatc catccctttg taaataagga cagaatcaac gcttatcaac tctactggta
    10381 aaattttacg cagctcaatg gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa
    10441 aaatcgcctt aggcaggctc gccttactga tatcactcat ctttcagggc ttttatcgcc
    10501 ggatgtaaat caacagattg taacagacca cccggcggcg ttttcacaag ttgcggacat
    10561 aaccgttcca cgtccgccag cagggttatg gcctgtgcca ttgaccactg cgcatgttcg
    10621 ctcatcagat ttcgtgcaat ccactgcgca atattgccag gttcgaatac ggactgcttc
    10681 gccaggtagc ctatcagttc aggaatcaag atttgtaaat tttgttggcg taagggtaaa
    10741 ggcacggccc tgatggtcac atgctgtgca tctgactgga aatcaatacc caattccgct
    10801 agggcagact gcgctttttc taatgccgat ttttcttcgc cagaaacttt tagccgcaac
    10861 ggaatcagca gcggctgggc gcaaacgggc gcttcacccg gcgtcaattg tgcctgacgc
    10921 agccaacgtt ctgccactgg caaggataaa agtgaaatgt tgccatcgcg ctccagcaac
    10981 gcacagtcgg aatggacgat agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt
    11041 gcaggttcct gcggttccgg cgcttttggt ttttgcatcg gcgcgggcgt ttgcaaaagc
    11101 tggcgataca cttcaccttg ctgtttctgg tagcctggct gcgcattcgg ccagggggca
    11161 gccggacgac tgcctgaggc gggcgcagga gagtagcgcg gagctaccgg ctcacgagct
    11221 gccggttctg caaagtgatt gcgccccgcc gccacgcggt tttccgggat ggcacgcggt
    11281 gcgggttgtg gttcatcgtc cagcggtagc ggcgtttcca gttgctgttg tagcacgctc
    11341 agcacgccct gatagataaa atcatgcacc agacgcgact gatggaaacg cacttcgtgt
    11401 ttggcggggt gtacgttgac gtccacctga tgcgggtcga tctccagata caacacaaat
    11461 gccggttgct gatcggcccc cagtttgtct tcgcaggcct ggcggatcgc atgattgatc
    11521 aggcgatcgc gcatcatgcg accattcacg tagcaatact gaatttccgc cagtgcgggc
    11581 gtagtgtgat ttggatcggc cacccagccg cgcagggtga gatcgccgtg ttgccattca
    11641 atcgccagcg cttgttcgag aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt
    11701 tgcccgcctt ccggcactgc gcggtactga cgcacaattt taccgttatg cgacaggttg
    11761 atcgtgacgt cgaaacgcgc cagcgcaatg cggcggatga tttcatcaat atggctaaat
    11821 tcggttttct cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc
    11881 acctccagcg tcgttcccac cggatgcgct gccggtttaa ccgtcacgtc catgtcgcgt
    11941 ccttcggcat aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg
    12001 cgggaaaccg aactgatact cgccagcgcc tcaccgcgaa agcccaggct gataatggct
    12061 tcgagatcgt ccagagaggc gattttactg gtggcatgac gagccagcgc cagcgccagc
    12121 tcatcttttt tgataccgca gccgttatca cgaatgcgga taagtttcgc cccaccgcgt
    12181 tcgatatcaa tatcgatacg cgtcgcacct gcatcgaggc tgttttccac tagttctttg
    12241 actaccgacg caggtcgctc gaccacctca cctgcggcaa tctggttcgc cagttgtggc
    12301 ggtaagacct gaattggcat caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc
    12361 accgtactgg cagtttgtgc cgttgcaccc tgcggcgcag attgcatcgg atgcgcaagg
    12421 aagtaattgc gcaggccttt gtaaatggct tctgccagct gttgttggta atcgtcgctc
    12481 gccagcaaac gttcttcgct gttgttgctg ataaaaccgg tttcgaccag tactgatggg
    12541 atatccggcg agcgcagaac gccaaggctg gcgtgttctg gtcgacgttt atgtatttcg
    12601 ccaatgcgtt gcaactgact gatcatactg gtcgctacat cataccctac ccgctgggaa
    12661 tgaccgaact gtaaatccag caccgcctgg cttaaatagg ggtcagactg actgttcgcc
    12721 agcacatcac ccgccccacc cagcagctcc gacagtttct cgtgctgctc cagccagctg
    12781 gccatttcac tgttggcgcg acggttagag agcacccata cggaagcgcc agtcgcacta
    12841 cggttcggtg cggcatcagc gtgaatcgac acgaggaaat tggcgttttg cttatgtgcc
    12901 acatcactgc gccccatcac cgagataaag taatccccgt cacgggttaa aacgccttta
    12961 aacatcggat cgtcattgag caaagtacgc aatttacgcg cgatggcgat ggtgacattt
    13021 ttctcccgcg taccaccggg gccgatagcg ccagggtcct gaccgccgtg tccggcatca
    13081 atagcgatga taattttatc gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc
    13141 gtattactgc tgataacacc cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc
    13201 ggttcgctga cgcgcggtgc gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc
    13261 ggcggaggag gcggtggcgg cacatcggcg ttaatcgtaa agacgacagt gtaattgctg
    13321 ccattctgcc gcttcaccgc ttcggtttta ccgttttcgg taagatcgac caccagccgt
    13381 agcgtttgtg catctttagg cgttccagag cgaatcgcct tcaccagatt attgccgctg
    13441 aacaacaacg gcagtccctg aatcatgccc gtttgtttga tatcgagcgc cacggtgcgt
    13501 ttgctttgat ggctaaacgc ataatcagga tcgccaataa aactcaacgt tatccgcgcc
    13561 tgttgattac cgttagaaac ctgaatatca gagagcgtcg cggcacccac cggcgtgcac
    13621 agcagcagca gcgtcgctac caaccaattt ctgatgcgat acatcatccc gccacctttc
    13681 aaaggttaac cggctaaacg cgccagcaac aattcacccg cagaggaaac cgcactcacg
    13741 cgcgcctcac ggccttgtgc ctgataatca atgtgtattt cgacatccgg gtcaggaaga
    13801 acacctgtac cttgttgtgg ccactccacc aggcagatgg catcgttggc aaaataatcg
    13861 cggatcccca taaactccag ctcctcggga tcggcaaggc ggtacaaatc aaagtgatag
    13921 accattaagt tgtcgagcgt atagggttcg accagcgtat aagtggggct tttgacatta
    13981 ccctgatgac ccagagcctg taaaaagccc cggctaaagg tggttttacc tgcgcctaaa
    14041 tcgccataca gatagattac ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg
    14101 tctaatgttg cctgctcatc agggagcgga attactcgat tcatcatggt ttttatcagt
    14161 cacttccggg ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca acatcccgcg
    14221 cgttccaaaa cgcgccgcca gtacgtcagc tgccgcaccg tgcgcgacac agcctgcaca
    14281 ggctgcatca tacggcgaca gtttttgccc aagcaatgcg ccaataatac cagagagcac
    14341 atcgcccatg ccgccgctcg ccatgcctgc atttccggca tcaataatgc ctaaagcgtc
    14401 aggatgggcg gcgaccacgg ttccggcacc tttcagcacc gctacgccgc cataacgttg
    14461 taccagacgt ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg aacagcctaa
    14521 caaccgtgcg gcctcgctag gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt
    14581 gattgccagc aggttcaatg catcggcatc ccacaacatc ggtttgcgaa aattctcaac
    14641 tttttgcagg gcttttttcc cccactcttg ctggcccaga ccgggaccaa tcaccaccac
    14701 atcggcccat tccaggcttt cggtaagaga gtccatagtc agttcatgca ccatcaattc
    14761 tggtcgtgca gtcagcagcg gcgcaatgtt ctcactgcgg gtcagtactc ggactaaacc
    14821 agcaccagca cgtagcgccg cttcccccgt catacgaata gcccccgccg tgccgtgatc
    14881 gccaccgata atcaccagcc gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt
    14941 cagccagtga gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag
    15001 ccagctatcc agccccagtg agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc
    15061 agtgagcaag cctagtttca gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc
    15121 gcctggcgta gcgccggttt cagccagcag gccggaaggg atatcaaccg ccacaatcgg
    15181 cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg
    15241 caagccggta ccgagcagcg catcaacaat cagatctacc gattcgggcc agacaatatt
    15301 cgaagcatgg atctcgccac ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc
    15361 cggcaacggt ttgtcgctct cctgggccaa caacgtgacc tcaatgccga ccgctttggc
    15421 cagtcgcgcg accacgtagc catcgccgcc gttattacca tgaccgcaca gcaccagcca
    15481 gtggcgggcg tcaggatacg ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag
    15541 catcagctca tagagtgtga gccccagcac atctgccgcc tcgcgttctc cgcggcggat
    15601 atcgtcggcg taccagacgg tgtgtggtat acttacgggg tttttcttca ttgtatggtc
    15661 cgtcatgtca gagcccctcg atctcaatca gttagcgcaa aaaattaaac agtgggggct
    15721 ggaactgggc tttcagcagg taggtattac cgataccgat ctcagcgagt ccgagcccaa
    15781 actgcaagca tggctggaca aacaatacca cggcgaaatg gactggatgg cacgtcacgg
    15841 tatgctgcgc gctcgccccc atgagttatt acccggtacg ctgcgtgtaa tcagcgtgcg
    15901 gatgaattac cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg
    15961 ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca accgactcaa
    16021 aaagctgggc gagatgattc agcaacattg tgtttcgctg aattttagac cgtttgtcga
    16081 ttctgcgcct attctcgagc gcccgttagc tgaaaaagct gggctcggct ggacaggtaa
    16141 gcactcactt atcctcaatc gcgaggccgg ttcgttcttc tttttaggcg aattgctggt
    16201 cgatattccg ctgcccgtgg atcaaccagt cgaggaagga tgcggcaaat gcgtggcctg
    16261 tatgacgatt tgcccgaccg gtgccatcgt cgagccatat accgtcgatg ctcgccgctg
    16321 tatctcttat ctcaccatcg aacttgaagg ggcgctcccg gaagagttgc gaccgttaat
    16381 gggaaaccgt atttacggtt gcgatgactg ccagcttatc tgcccgtgga atcgctattc
    16441 gcaactcact acagaagacg atttcagccc gcgtaagccg ctacacgcac cggaactcat
    16501 tgagttattt gcctggagcg aagagaagtt tttaaaagtc acggaaggat cggcgattcg
    16561 ccgtataggt cacctgcgct ggctgcgtaa tatcgccgtg gcattaggca atgccccttg
    16621 ggatgaaacg attttgacag cgctggaaag tcgtaaaggt gagcacccac ttctcgatga
    16681 gcacatagcg tgggcgattg cgcagcaaat agagagacga aatgcgtgca tagtcgaagt
    16741 gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc
    16801 ctgattcatc cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa
    16861 atcgtcaagt gatcgactcg acaaaacgga atcttgattt ttaacttatt tatcaatagg
    16921 ttaaaaaaat accattcacc gtgcgaagtt tctttgaaat taagattaca gcctgaggct
    16981 gtggataact ctgtttacaa gagtttgtca gagacaacaa aaaccagcaa taacgcatcg
    17041 cgtcgctgta gatattttat tgagagaaga
//
LOCUS       DAJTNP010000083        16428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTNP010000083 DAJTNP010000000
VERSION     DAJTNP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..181)
                     /locus_tag="OO160_002973"
     CDS             complement(<1..181)
                     /locus_tag="OO160_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5525011.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSV"
     gene            complement(299..1738)
                     /gene="rsmF"
                     /locus_tag="OO160_002974"
                     /pseudo
     CDS             complement(299..1738)
                     /gene="rsmF"
                     /locus_tag="OO160_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(1818..4438)
                     /gene="yebT"
                     /locus_tag="OO160_002975"
                     /pseudo
     CDS             complement(1818..4438)
                     /gene="yebT"
                     /locus_tag="OO160_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4407..5690)
                     /gene="yebS"
                     /locus_tag="OO160_002976"
     CDS             complement(4407..5690)
                     /gene="yebS"
                     /locus_tag="OO160_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5525012.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5820..6317
                     /gene="msrC"
                     /locus_tag="OO160_002977"
     CDS             5820..6317
                     /gene="msrC"
                     /locus_tag="OO160_002977"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5525013.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6414..7112
                     /gene="proQ"
                     /locus_tag="OO160_002978"
     CDS             6414..7112
                     /gene="proQ"
                     /locus_tag="OO160_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5525014.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7132..9180
                     /gene="prc"
                     /locus_tag="OO160_002979"
     CDS             7132..9180
                     /gene="prc"
                     /locus_tag="OO160_002979"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5525015.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9372..10253
                     /gene="htpX"
                     /locus_tag="OO160_002980"
     CDS             9372..10253
                     /gene="htpX"
                     /locus_tag="OO160_002980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5525016.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10299..11672)
                     /locus_tag="OO160_002981"
     CDS             complement(10299..11672)
                     /locus_tag="OO160_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5525017.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            11849..12640
                     /gene="kdgR"
                     /locus_tag="OO160_002982"
     CDS             11849..12640
                     /gene="kdgR"
                     /locus_tag="OO160_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5525018.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12783..13022)
                     /locus_tag="OO160_002983"
     CDS             complement(12783..13022)
                     /locus_tag="OO160_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525019.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13181..13324
                     /gene="mgrB"
                     /locus_tag="OO160_002984"
     CDS             13181..13324
                     /gene="mgrB"
                     /locus_tag="OO160_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5525020.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13399..13686
                     /locus_tag="OO160_002985"
     CDS             13399..13686
                     /locus_tag="OO160_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5525021.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14356..14499
                     /locus_tag="OO160_002986"
     CDS             14356..14499
                     /locus_tag="OO160_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5525022.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14512..14721
                     /gene="cspE"
                     /locus_tag="OO160_002987"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14512..14721
                     /gene="cspE"
                     /locus_tag="OO160_002987"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5525023.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14887..15696
                     /gene="rlmA"
                     /locus_tag="OO160_002988"
     CDS             14887..15696
                     /gene="rlmA"
                     /locus_tag="OO160_002988"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5525024.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15693..16259)
                     /gene="mntP"
                     /locus_tag="OO160_002989"
     CDS             complement(15693..16259)
                     /gene="mntP"
                     /locus_tag="OO160_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5525025.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4299 a         3993 c         4177 g         3959 t
ORIGIN      
        1 ggacagagtg ttcgccattt agcagggcag ggatactggt ttcagcatcc caggcatcca
       61 gttgcataca taaatcgggg tcagatttac aaggaatggt taacgttcga tcacccggcg
      121 attcaccgtg taattcgccg tcgtcgattt caaaagcgcc tatgcgcaca ctggttttca
      181 tcttcgtgct ctccatttag ctgctgattg tagtacgacc agtccgatcg cacaattttt
      241 cagcctagtt gaggccggcg aatgtgccag tcaaaaagtg cgcttttttt gtgcgctgtc
      301 atgcgttact ggtaaaaagt ttcccgtccc gcaccagttc acgcgggtag ctgtttttca
      361 gtcgcgaacc aatccgtttg gctaaaccaa tcggctgatg ctggaaagta accaacacgt
      421 catccgccac tggcgcggct tgcgggtaaa catcgcgccc gcgataccac tcctacgctt
      481 cctgcggtgt cagttcaaaa gcgtttacat tgtcggggga ggcatgggca ataaccgctt
      541 catgctgcca gcgataacct ttgttatgcg tttcggcaag tttaatccct aagcgagaaa
      601 atcggacttt accgatcagg gcttcaatgc ctaccgggaa caaccacagt tctttgtcgc
      661 gttgccagag tcgcaggttt ccatcccagt ttaagccaac acttgcagcc gcctgacgaa
      721 tttgtccggc ttcgcgatct ttcaccgggc tgaacgggaa attgcccact ttgtattttg
      781 gggcgggtaa ggcggggatc gcctgagttt tacgcagacg agcaacgaag aagccttcgc
      841 agtcgtaaat ttgtgggaaa acatgcaaaa aaccttcttc ggtcagcgcc ttgtttgcac
      901 cagggaagag atcgccaagt ggtaaaaact ctacttcgtc ggggtaagtc tctttcagcc
      961 accggcaaac ggcttcgttt tcttcccggt ttaaggtata ggtcgagtaa accagcgtac
     1021 cgccaggacg taatgcatga aaggcgctgt caatcagttc ccgttgggta gctgcgattt
     1081 cctgattgct ttcgggtgac cagtttttta gcgcatcggg atctttacgc accacgcctt
     1141 cgccggagca gggagcgtcc agcaaaatgg catcaaacat ttctggcact gccgcaccaa
     1201 acacgcggcc accaaaatgt gtgagcgcaa cattactgat gccacagcgg ctgatattgg
     1261 catgtaacac ttttacccaa ctggcggaaa actcattggc aaggattgcc ccttcgttat
     1321 tcatccgcgc ggcaatttgc gtcgtttttg agcctggcgc agcagcgaca tccatcaccc
     1381 gctgtggtgc attaccgtca gcaaacaagg cggcgacggg caacattgaa ctggcttcct
     1441 gaatataaaa caggccactt aaatgctcgg tggtactacc caatggcaat gcatcttcat
     1501 tgtcgcgttc aatccagaaa ccttcttcac accacggaat tggcgtaagc gtccagccat
     1561 aaggagcggt taattgcagg aaatcagcaa cggagatttt cagcgtatta acgcgaatgc
     1621 tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc aaatgagagc gtcgaaggca
     1681 tagcttcgcg catttgtgtc agaaacgcgt ccgggaaata aacggtgtgt tgggccacga
     1741 gcatgtacca ctggcaaaaa aacaggcgcg cagtttagca taagcgctcc ggcgcaggca
     1801 cgccggagca gtgggcatta tttgggaagc gcagttcccc attcacgcca ctctttcggt
     1861 tcactttcct gcaacaggaa gtgtttgcct tcttgggctt tcggtgcaag tggcgtaccc
     1921 ggaggcgtgg cgaaggcgat gccgccacgg ataaattggt taaaggtgcc ggttttcact
     1981 acgccgcccg tcagaccaaa gtccagactg taacccgatg ccaaccagaa gacggaattg
     2041 ttacgcacca ggtgttgata gcgtttactg atgcgcatcg caatcatcac gcgatctgac
     2101 aatgtcccca gcgtcattcc ggtaaccgta cctacttcca gaccacggaa cagcacaggc
     2161 gtaccgatgc ctaacgaacc ggcttccggc gcttcaacaa taatgcttaa accatctagg
     2221 taacgcgaat cagtaatggt ggcctcttct aattcaaagt cgcgacgagg attgccacgg
     2281 cctggttcga cgttgatata cggctggagg atagtatcta gatgctcaac gccagctgcc
     2341 gagatttgcg gtgtgaccac tgagaagcgc gtaccaccgc gagcaaaggt ctggacatat
     2401 tccggataga gcaccgcctt tgcctgtact tcattgcgtg cggtaatcag atccagcgtc
     2461 tggatttgcc cgatatcaat accgagatag cgaattggca tcccgaccgc cagttttccg
     2521 gcatcgaaag cgtgaagcgt aatctgcctg ccaaccgcac gggccgctgt ttcggaagca
     2581 tacagaattc gcttgtcgcc tttacgctga ctggcgctgg caccactgag gttatcgaag
     2641 ctaatggccc cctttaatgc tctggagagc ggggatgcct gtacggtcag gccactacca
     2701 ttcagctgaa ctttcgcccc gccttctgcc cagaacacgc tgttgctggt caaaaggttg
     2761 cgatattccg gtttaatgtg cagatcgata tcgaacgcgt tagctcgcgg acgcacggta
     2821 ataacttcac caacttcaaa tttacggtac agcactaccg atcctgcctg cacatccggc
     2881 agcgtctctg cactcaaact catggttgtg gtgggtaaat cgctaaggct gttctccagc
     2941 gctttttcca gattggcata cagtggatag ctggctttca tctcgccttt atcgcccggc
     3001 agaatacgta tcccgccgtt aatccattct gaggcgctgg caccgagaaa ctcaacgcca
     3061 tccagcccca ccttcacgtc gacacggctg ttgacgacaa atttgctatc gccttttacc
     3121 agttctcgat gctgaggctc gatggcgacg gtaaaggtga cgcctttgct ggtgagttta
     3181 cgatcgataa cctggcctac ctgcacgccg tgaagaatga gcggctgacc cgcatcaata
     3241 ccgtaacttt ccggtgctgt cagggtcagt gtcagaacgt ccggctcttg cagcagggct
     3301 ttttcgcccg gcacaacaac gaactctttg cgtggctcgc catcgccggg caccaactca
     3361 aaggtttttc cggtcagcag ggcgctgaga ttagcatcgc taagggataa tttcgggttg
     3421 cgtaattcga tgcgggtatt ttcacgaagc agggatcaac ggtcatttcc ccagtgactt
     3481 taccaccagg atttaaatcc agtttagtca gctgtccgac ttccagcccc tgatacatta
     3541 acggcgtcga gtcggcggtt aatcctgctc cactcggcag ttccagtttt attattactc
     3601 cacgctggct gtgggccaga tcttcataca gaccaaaggt atcttccgcc tcggcaggtt
     3661 tcgactcttc aggtgaatcg aaggcaatcg caccgttaac cagtgccgcc agactttcca
     3721 gtttcacctt cgcgccactg atactgacgt tggcatcaac gccggaaacg tttcagaaac
     3781 ggctaccttt tttcaccaga tcggtaaaac gccgctcgat caggacatca atcaccacgc
     3841 cttgcttgtt gggattgatg gcatagtcgt agacttttcc caccgggatc ttgcggaaat
     3901 agaccaatga accgctgttc agcgaaccga gagcgggggc ttgcaggtgg atcatcagat
     3961 cgccattgtc cagccgatat ttcggttggg tatcgagcgc gacaaagtga tcctgctctt
     4021 tacctttacc cggcatcatg ccgatatagt tcccaccgac gagggcgtcc agcccggaga
     4081 cacctgccaa cgatgctttt ggcgtcatca gccagaactg agtctcttcg cgcagcgcat
     4141 ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg tcgctgaggc
     4201 tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta cggcccggaa
     4261 caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc tgataactgt
     4321 cccaaatcag ccaaccggca atcattagcg cgatgaaagg cagcagccag aaaggtgaga
     4381 tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt
     4441 gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa aattacggca
     4501 gcgccaaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc gcgattaatc
     4561 agcgacatgg ttaaagatat gacaaacagg tcgagtatcg accagcgtcc aatccaggtc
     4621 accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc
     4681 aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact ggcgataaac
     4741 acgattcccg caaccgcaat gttgctactt gccagcgaca taattccgga aagaatcgta
     4801 tcttcctgcc ggccaccatt cagataaatg atagaaatag gcaataggtt ggctggtaac
     4861 aacaaaacga ttgacgctaa cagcgccgcc cagcattttt gcaaactatg acgacgacgc
     4921 aggcgtagcg ggatatggca acgcgggcag agaccacgct gatcaggata gccggtaaaa
     4981 tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg ctgcggataa
     5041 tatcgctccc acagctcctc aacattaaga tgtgacaacg tcaccgtcgt taaaatcacc
     5101 aacgccacaa aagagaacaa gccgacaccc gcctggatat gggcataatc ctgtaccttt
     5161 atagaagcaa cgccaatgcc gaccaggtag atatccagca ttacccactc tttaagtcgc
     5221 tcaagcatca gcagtaccgg acgtaaattc attcccagtc ggttaccaaa ccataaatat
     5281 gctatggagg acaccagaat gaggggggca ccaataacgc agaaaaagac cattgatccc
     5341 gttatcgcat cgccctgttt ggtcatttgc cagatgcctt gcatgacgtt ggcgtcaata
     5401 cgaatgccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg cataagcaac
     5461 agcatggtga aggccattgc cgccaggcgc gttagcgacc agtcgcgccc gtcgcgaatt
     5521 tttgcctgac agcgcggaca ataggcactt tgatgagaat ttatctcggg caggctaaac
     5581 agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc gccgattgcc
     5641 ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat gacgtgccaa
     5701 atgtaataag aattaacatt aatcttaacc catgatggga atgatcttga gcattaacgc
     5761 atttaatgct tattttaata ggctggcagt tccaggtaag acaaccaagt gattaagtaa
     5821 tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg atggcgggag
     5881 aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatacgag cgtctcactg
     5941 acgttaactg ggcaggtttt tatttgcttg aggacgatac actggtactc ggaccatttc
     6001 agggcaaaat tgcctgtgtc cggatacccg tcgggcgcgg ggtgtgcggc acagcggttg
     6061 cccgcaatca agtgcagcgt atcgaggatg ttcacgcgtt tgacgggcat attgcctgtg
     6121 atgcggcgag taattctgaa attgttctgc cgctggtggt gaaaaatcag attattggtg
     6181 ttctcgacat cgatagtact gtcttcgctc gctttacaga cgaggacgag caaggcttac
     6241 gtcagcttgt ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa aaattctttg
     6301 cgagcgtcgc aggataatca acggataacg tagcatttac tggtagcgtc attataatga
     6361 cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat ttcatggaaa
     6421 atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa cgttttcccc
     6481 actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat tggtattttt caggatttgg
     6541 tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc gctttacgtc
     6601 tctacacttc gagctggcgt tatctttacg gtgttaaacc cggcgcaacg cgtgtcgatc
     6661 ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct cgcaagcagc
     6721 ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg aaaaaacgcg
     6781 aagctgccgc agctgctggt gagaaagaag acgcaccgcg ccgcgaacgc aagccacgtc
     6841 cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa aagccggtag
     6901 agaaagcgcc aaaaacagta aaagcacctc gcgaagaaca gcacaccccg gtttctgaca
     6961 tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac gcgatggatg
     7021 ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg ggtatgtctt
     7081 tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggctagg catgaacatg
     7141 ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac cttcgctgta
     7201 gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca gcatgcgacg
     7261 gtaagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt cgacctcgat
     7321 caggcatttt cggccaaaat ctttgaccgc tacctgaatc tgctcgatta cagccacaat
     7381 gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt aggcgatgaa
     7441 ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca aaagcgccgt
     7501 tttgaacgtt accagtacgc tttgtcggta ctggaaaagc cgatggattt caccggcaac
     7561 gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc tgagttgaac
     7621 gcgcagtggg acagtaaagt caaattcgac gagttaagcc tgaagctgac aggaaaaacg
     7681 gataaagaaa ttcgtgaaac cctgacacgc cgctacaaat ttgccattcg tcgtctggcg
     7741 caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg tgaaatcgat
     7801 ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga aatgagtttg
     7861 tcactggaag gtattggcgc agtgctgcaa atggatgatg actacaccgt tatcaattcg
     7921 atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga caaaattgtc
     7981 ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct tgatgatgtg
     8041 gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt acctgctggt
     8101 aaagggacca agacccgtac tgtaacgttg acccgtgaac gtattcgtct cgaagaccgc
     8161 gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct ggatattccg
     8221 ggcttctatg tgggtttgac agacgatgtc aaagttcaac tgcagaaact ggaaaaacag
     8281 aatgtcagca gtgtgatcat cgacctgcgt agcaatggcg gtggggcgtt gaccgaagcg
     8341 gtatcgctct caggtctgtt tattcctgcg ggtcccattg ttcaggtccg cgataacaac
     8401 ggcaaggttc gtgaagacag cgataccgac gggcaggtgt tctataaagg cccgctggtg
     8461 gtactggttg accgtttcag tgcttcggct tcagaaatct ttgccgcggc aatgcaggat
     8521 tacggtcgtg cgctggttgt gggtgaaccg acgtttggta aaggcaccgt tcagcaatac
     8581 cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc gctgggttct
     8641 gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca acgtaaaggc
     8701 gtaacaccag acatcatcat gccgacgggt aatgaagaaa cggaaacggg tgagaaattc
     8761 gaagataacg cgctgccgtg ggatagcatc gatgccgcga cttatgtgaa atcaggagat
     8821 ttaactgcct ttgaaccgga gctgctgaag gaacataatg cgcgtatcgc gaaagatcct
     8881 gagttccaga acatcatgaa ggatatcgcg cgcttcaacg ctatgaagga caagcgcaat
     8941 atcgtttctc tgaactacgc tgtgcgtgag aaagagaata atgaagatga tgcgacgcgt
     9001 ctggcgcgtt tgaacgaacg ctttaaacga gaaggtaaac cggagttgaa gaagctggat
     9061 gatctatcga aagattacca ggagccggat ccttatctgg atgagacggt gaatatcgca
     9121 ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgctcc cgtcaagtaa
     9181 tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat gccgtaaatc
     9241 agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc cagacttgaa
     9301 aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta aactgaggta
     9361 aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc gtaatggtcg
     9421 ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag gggctgatga
     9481 tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg tccaaatgga
     9541 tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa agggaacgtt
     9601 ggctggtcaa tactgtagca acccaggctc gtcaggcggg gatcgctatg ccgcaagtgg
     9661 ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt gatgcctctc
     9721 tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc gaggcggtaa
     9781 ttgctcacga aatcagccac atcgccaatg gtgatatggt caccatgacg ctgattcagg
     9841 gcgtggtgaa caccttcgtt atctttattt cccgtattct ggcgcagctt gccgcgggtt
     9901 ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg atctactttg
     9961 cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc accatgtggt
    10021 tctcgcgtca tcgtgaattc catgcagatg ccggttcggc aaaactggtt ggtcgcgaga
    10081 aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa gcaaccagca
    10141 tgatggcttt ctgcattaac ggtaagtcga aatcgctcag tgagttgttc atgactcacc
    10201 cgccgctgga taaacgtatt gaagctctgc gtacgggtga atacctgaag taatatgaag
    10261 taaatactaa aaagcgcgtc ctgatcgacg cgcttttttt atgccctggc tcgtggctga
    10321 gtgatacgta aaccactgac acaggcagca atcactgcca gaatagccgc agccatcagc
    10381 gagacgtgcg taccattatc accaaactga tttagcatca gcgccaccag cgccgcgcca
    10441 ctactctgac ccagtagacg agccgttcct aacatgccac tggccccacc gctacgttcg
    10501 cgcggagcgg aggtaataat ggtgtggtta ttgggtgact ggaataagcc aaatccagcg
    10561 ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg cagcagaacc
    10621 agggaaaaaa gccccgtagc catgatgaac aaccctaaag cacccagcaa tcctgcatgt
    10681 acgcgttcaa tcaaatagcc tgccagagga gccatcacca tcgttgctaa cggccacggt
    10741 gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag gtaaaagggc
    10801 agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca aatagaaagt
    10861 gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtacgggaag agaaagctgg
    10921 cggcgaataa agaaaatacc aacaacaacc attaccacca gttccgcagc aattaacgtc
    10981 agcgattgcc cctgagcgaa accactcaac gcagtgataa gcaggccgaa ggttaacgcg
    11041 ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cgcgagaacc attgggtggc
    11101 agaaaacgca tcgccagaag cagggcgata atacctaacg gtacgttgat taaaaataac
    11161 catttccagg atgcgatgga gaggattgct gcagcaattg tcggcccggc agcagaagag
    11221 acggcaacaa taaacgagtt tatgcccatc cctctcccca gaaaacgttg tggatagatc
    11281 aggcggataa gtgcggtatt aacgctcatc aacgctgcac cgccgaaacc ttgtatgaca
    11341 cgcgcaaggg tgagcatttg cagcgaatca gaaagggcgc agaacagtga agacaacaga
    11401 aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcgcc cagaaacgaa
    11461 aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca gatggaactg
    11521 gctggtgtgg catgaaggtc cgtggcaatt gttggcaggg cgacgttggc gattgcgccg
    11581 tcaaggacag ccatcgaaat accaatcaca atggttaata tcgcaccgta tcgctggggc
    11641 aatggcaggc cgtcggcctg aacttttggc ataggaattt tatatctttg gtgaataatc
    11701 aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt gtaactaacc
    11761 ggcaaaagat ggattgcgac caccctggtg cgaaattata ataaaaaaca gttctgattt
    11821 ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata aacagcctga
    11881 ttctgtatct tccgtgctaa aagtttttgg cattttgcag gcgctgggtg aagagcgcga
    11941 aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg tttatcgctt
    12001 tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg agaaatattc
    12061 cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg atttaattcg
    12121 tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta tccacctcgg
    12181 cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt acaatttgcg
    12241 catgtattca cggattgggc gtcgtaatcc gctgtacagc accgcgattg gtaaggtact
    12301 gctggcatgg cgcgatcgcg atgaagtgaa gcaaattctt gagggcgtgg agtataaacg
    12361 cagtaccgag cggaccatca ccagtacaga agcgttatta cccgttctgg accaggtgcg
    12421 cgagcagggg tatggagaag ataacgaaga gcaggaagaa gggctgcaat gcattgcggt
    12481 accggtattt gatcgctttg gcgtggtcat tgccggtttg agcatctcct tcccgacgtt
    12541 gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg cagcgcgcaa
    12601 aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag aacctgtcgg
    12661 aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa ggtgcgttat
    12721 attaaacgcg cttgctataa gaatatttta ctcatgagtg agaatctggt tatttattac
    12781 ccttaaccat tatcgaccac gatattgctt ttacgtaaca gtgggcaatc tgttatctcc
    12841 aagaaaccac ttttagtgtg caagtattgt accgtgctgg tgcctctggc agtcagatag
    12901 gtacattgca aacctaatcc tgcggcattc tctttgcttc caatcaaaac gccatatccg
    12961 ctgagtaata atcctatcca taccagtgct atcagcataa ctgtgcgaat gatgaatcgc
    13021 attacaatct cttctctttt tatgttcgct taatcgtagc ggcaatatgc gctgaagcaa
    13081 gctactcatt ccgaaaaagc acgaatatcg acatagttag gcgctgttta actaacgcat
    13141 gctagtttaa tgacataagg taggtgaagc ggagattgga gtgaaaaagt ttcgatgggt
    13201 cgttctggtt gtcgtggtgt tggcttgctt gctgctttgg gcgcaggtat tcaacatgat
    13261 gtgcgatcag gatgtacaat ttttcagcgg aatttgtgcc attaaccagt ttatcccgtg
    13321 gtgatatcat tttttcagca ctagattttc ttcctctcca gcagtggtag aatagcgcct
    13381 ctactcagag gtggtgaaat gaacgaagtt gtaaattcag gcgtgatgaa cattgcgtct
    13441 ttggttgtat cggtggtggt tcttcttatc gggctcatct tgtggttttt tatcaatcgt
    13501 gccagttcac ggactaacga acagattgaa ctgcttgagg cgttgctgga tcagcaaaaa
    13561 cgtcaaaatg cactgttacg tcgtttgtgc gaagcaaacg aaccagagaa agcagataaa
    13621 aagaccattg agagtcaaaa atcggttgat gacgaagata ttattcgcct ggtcgccgaa
    13681 cgataatcct tcctcaatct tgagtaaccc cgtcgttgaa gggttactca attcatcctg
    13741 attatacgca ctgtcatggt gcaactgctt caaattaaaa ctctggtctg acttcaaata
    13801 attatcatat tatttatttg cgtaacggga tatcagaaaa agatattttc ctcattaatt
    13861 tattaggatg tttacatcag atttgtgatt aagcgtggta ttatttatta gcgaaacgtt
    13921 tctctcttga tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt
    13981 ggggattgac ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa
    14041 gtgatataac tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat
    14101 aacttctccc tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag
    14161 tttaagtatc tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt
    14221 tgcataagtg tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagatat
    14281 gtttaaaaat ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga
    14341 ggtacagttc tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac
    14401 gttgaatacc gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt
    14461 ttatctatgt tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag
    14521 attaaaggtc aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct
    14581 gatggcagca aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact
    14641 ctggctgaag gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct
    14701 gttaacgtaa cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct
    14761 cgctataaag cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt
    14821 tcggatattt gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgccgga
    14881 gttgtcatgt cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc
    14941 tatatctgtc cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg
    15001 cccgttcagc ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc
    15061 cgcgcattct tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg
    15121 cgggaacggc ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat
    15181 tacacacacg catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg
    15241 aaggtagcga taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc
    15301 agccaccgtt tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg
    15361 tgtaaagcag aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg
    15421 ccgggaccgc gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat
    15481 gcacctcatg cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat
    15541 ccgatgcgtc ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg
    15601 cgtgcgaagc cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg
    15661 gactttaata ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc
    15721 tggacgccga tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt
    15781 gagccgataa agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca
    15841 atggccaatg cggtcgcgat aatgttgacc tgaaggaaag caagaccaac acccacagcc
    15901 atggcatcca ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg
    15961 cgcggctctt catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg
    16021 aggaatatca gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct
    16081 aacatgccca ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg
    16141 ccggttcgca atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat
    16201 gcagcaaatg catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg
    16261 acaacgtcct gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca
    16321 tgaacaacaa gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat
    16381 ggtctcgcct gactgaaagt cttcagtccg tacgtgccac gttttgca
//
LOCUS       DAJTNP010000084        16391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTNP010000084 DAJTNP010000000
VERSION     DAJTNP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO160_002990"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO160_002990"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5525026.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO160_002991"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO160_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5525027.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="OO160_002992"
     CDS             2341..2556
                     /locus_tag="OO160_002992"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525028.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="OO160_002993"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="OO160_002993"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5525029.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /gene="galP"
                     /locus_tag="OO160_002994"
     CDS             4451..5845
                     /gene="galP"
                     /locus_tag="OO160_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5525030.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="OO160_002995"
     CDS             5922..6419
                     /locus_tag="OO160_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5525031.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="OO160_002996"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="OO160_002996"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5525032.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="OO160_002997"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="OO160_002997"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5525033.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="OO160_002998"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="OO160_002998"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5525034.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9104..9667
                     /locus_tag="OO160_002999"
     CDS             9104..9667
                     /locus_tag="OO160_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5525035.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="OO160_003000"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="OO160_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5525036.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="OO160_003001"
     CDS             complement(10259..11239)
                     /locus_tag="OO160_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5525037.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="OO160_003002"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="OO160_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5525038.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="OO160_003003"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="OO160_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5525039.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="OO160_003004"
     CDS             12542..12832
                     /locus_tag="OO160_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5525040.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="OO160_003005"
     CDS             12840..13433
                     /locus_tag="OO160_003005"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5525041.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="OO160_003006"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="OO160_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5525042.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="OO160_003007"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="OO160_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..16387
                     /locus_tag="OO160_003008"
     CDS             15884..16387
                     /locus_tag="OO160_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5525043.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4061 a         4220 c         4124 g         3986 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctacgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgagtcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccaaggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg ccgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggac tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cggcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ctgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcccttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taactatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact tgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 tctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggtgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgagca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cttgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tatacaatgg tgctgttatt acgcatctgg atgcagtgtg
    12061 ctcattgtga tttttacacc cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgcc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtgtg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgactatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaatcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tggcgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 taaccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccac gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgccttg tgttttatta atcaaagcat tcaacaactg aaacaacctg cgcaggaggc
    16381 cagttaatgg a
//
LOCUS       DAJTNP010000085        15871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTNP010000085 DAJTNP010000000
VERSION     DAJTNP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            387..1445
                     /gene="citC"
                     /locus_tag="OO160_003009"
     CDS             387..1445
                     /gene="citC"
                     /locus_tag="OO160_003009"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5525044.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1460..1756
                     /gene="citD"
                     /locus_tag="OO160_003010"
     CDS             1460..1756
                     /gene="citD"
                     /locus_tag="OO160_003010"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5525045.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1753..2661
                     /gene="citE"
                     /locus_tag="OO160_003011"
     CDS             1753..2661
                     /gene="citE"
                     /locus_tag="OO160_003011"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5525046.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2672..4204
                     /gene="citF"
                     /locus_tag="OO160_003012"
     CDS             2672..4204
                     /gene="citF"
                     /locus_tag="OO160_003012"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5525047.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4208..4758
                     /gene="citX"
                     /locus_tag="OO160_003013"
                     /pseudo
     CDS             4208..4758
                     /gene="citX"
                     /locus_tag="OO160_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4733..5611
                     /gene="citG"
                     /locus_tag="OO160_003014"
     CDS             4733..5611
                     /gene="citG"
                     /locus_tag="OO160_003014"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5525048.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5662..7124
                     /gene="citT"
                     /locus_tag="OO160_003015"
                     /pseudo
     CDS             5662..7124
                     /gene="citT"
                     /locus_tag="OO160_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7238..8044
                     /gene="rna"
                     /locus_tag="OO160_003016"
     CDS             7238..8044
                     /gene="rna"
                     /locus_tag="OO160_003016"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5525049.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8273..8683
                     /gene="rnk"
                     /locus_tag="OO160_003017"
     CDS             8273..8683
                     /gene="rnk"
                     /locus_tag="OO160_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5525050.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8914..10152)
                     /locus_tag="OO160_003018"
     CDS             complement(8914..10152)
                     /locus_tag="OO160_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5525051.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10373..10801
                     /gene="uspG"
                     /locus_tag="OO160_003019"
     CDS             10373..10801
                     /gene="uspG"
                     /locus_tag="OO160_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5525052.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10922..12487)
                     /gene="ahpF"
                     /locus_tag="OO160_003020"
     CDS             complement(10922..12487)
                     /gene="ahpF"
                     /locus_tag="OO160_003020"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5525053.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12732..13295)
                     /gene="ahpC"
                     /locus_tag="OO160_003021"
     CDS             complement(12732..13295)
                     /gene="ahpC"
                     /locus_tag="OO160_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5525054.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13667..14413
                     /gene="dsbG"
                     /locus_tag="OO160_003022"
     CDS             13667..14413
                     /gene="dsbG"
                     /locus_tag="OO160_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5525055.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14623..15525
                     /locus_tag="OO160_003023"
     CDS             14623..15525
                     /locus_tag="OO160_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5525056.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15672..15871
                     /locus_tag="OO160_003024"
                     /pseudo
     CDS             15672..15871
                     /locus_tag="OO160_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3935 a         4107 c         3842 g         3987 t
ORIGIN      
        1 gcaacattgt tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt
       61 gaatcaaaat acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta
      121 aatcacttaa acctgcgagt catttcgtaa ttacattaat taaaaaactt aaaaccatta
      181 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc
      241 cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
      301 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      361 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      421 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      481 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      541 gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag
      601 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      661 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      721 cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      781 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga
      841 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      901 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
      961 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
     1021 cagaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
     1081 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
     1141 ttggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
     1201 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1261 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1321 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1381 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1441 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1501 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1561 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1621 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac tggactgcat
     1681 tttacatgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1741 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1801 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1861 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1921 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     1981 gtcaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     2041 gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tattgaaaaa
     2101 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     2161 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     2221 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2281 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2341 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2401 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2461 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccaccgc
     2521 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2581 aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt
     2641 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2701 acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc gatcttgccg ctttccagaa
     2761 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2821 ggaagaagcg attcgtcgct caggtttaca ggacggcatg acggtttcct tccatcacgc
     2881 tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt
     2941 taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     3001 cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc cactggcgga
     3061 agagatctcc cgtggtctgc tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt
     3121 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     3181 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3241 tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc
     3301 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttgatcg tcaaagttaa
     3361 tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc cgtatgacca ctaacccgcg
     3421 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactctggct acttcaaaga
     3481 aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga
     3541 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac
     3601 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc agagctttga
     3661 cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3721 gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3781 cgcgctggaa attgacaggc agttcaacgt taacgtgctg accggttctg acggcgtact
     3841 gcgtggcgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3901 cgcgccactg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     3961 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     4021 tccggaacta gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     4081 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     4141 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     4201 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4261 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc tggctcaagc accatcctgt
     4321 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg aggtcacacg
     4381 ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta gccacaaagc agggctggca
     4441 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt tgagcattgc
     4501 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4561 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4621 ttcactgcgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4681 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4741 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4801 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4861 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4921 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     4981 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5041 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5101 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5161 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5221 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5281 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5341 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5401 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5461 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5521 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5581 gatccttacc tgttttttag cacagattta attatttaag cacttgataa atttggaaat
     5641 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5701 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5761 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5821 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5881 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     5941 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6001 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6061 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6121 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6181 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6241 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6301 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6361 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6421 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6481 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6541 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6601 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6661 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6721 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6781 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6841 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6901 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     6961 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7021 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7081 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7141 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7201 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7261 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7321 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7381 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7441 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7501 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7561 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7621 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7681 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7741 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7801 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7861 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7921 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     7981 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8041 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8101 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8161 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8221 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8281 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8341 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8401 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8461 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8521 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8581 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8641 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8701 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8761 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8821 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8881 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     8941 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9001 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9061 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9121 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9181 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9241 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9301 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9361 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9421 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9481 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9541 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9601 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9661 tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt tcgggacgcg
     9721 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9781 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9841 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa tgacgaacgg
     9901 aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt caacgacttc
     9961 ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata
    10021 aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa tatcatctgc
    10081 ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt
    10141 caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct
    10201 atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt gttcagtgag
    10261 aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa
    10321 atattataat catggagaat gatttgttca tgattaacag ggagaaaggg ttatgtataa
    10381 ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca
    10441 cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc
    10501 agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca
    10561 acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg
    10621 cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga
    10681 actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga cccatctgtt
    10741 aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg tcgttcgcta
    10801 acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt tttaatgcca
    10861 taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt
    10921 cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt
    10981 cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca
    11041 cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg cggttacgtt
    11101 cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca aaaataccgg
    11161 ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga ccaacgactt
    11221 tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca
    11281 ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca aattccagca
    11341 gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg gagttaccgc
    11401 cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg
    11461 tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc cattttgcac
    11521 ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt
    11581 gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg tcgatcacat
    11641 caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc ccttcagtct
    11701 tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt
    11761 cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg
    11821 ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg
    11881 cacgattttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct
    11941 gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga
    12001 tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcaatacgc
    12061 tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc gagtaatagg
    12121 tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt
    12181 tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac tcgtggccca
    12241 gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc aggaaagacg
    12301 gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga
    12361 tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc gtggcaatta
    12421 actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt
    12481 cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct
    12541 ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt
    12601 aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga atccccggga
    12661 gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat ggcgtgaaag
    12721 acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    12781 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg agctgctttg
    12841 attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    12901 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    12961 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    13021 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    13081 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc agcaacgtca
    13141 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    13201 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    13261 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    13321 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    13381 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    13441 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    13501 cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac atggattaca
    13561 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga cagtgatagg
    13621 ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt taaaaaagat
    13681 acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt ccccagtaaa
    13741 agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg gaggaatgaa
    13801 aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc ccgatggtaa
    13861 gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta acacacttat
    13921 cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg aacaatccca
    13981 ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct ccgatccgtt
    14041 ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt
    14101 gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga cagcagcggc
    14161 aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct ctggttgcaa
    14221 gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt taagtgacaa
    14281 tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt acatgagtaa
    14341 ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta atatcattat
    14401 gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt ttcaatcatc
    14461 ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta gaaaaactta
    14521 atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa acattgatta
    14581 agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa tctctacgac
    14641 ttgaaaaagc tcgacttaaa tcttcttgtc atatttgagt gtatttacca gcatttaagc
    14701 attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca gtcgttacaa
    14761 cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg tatcgcgccg
    14821 acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg ccttgaacaa
    14881 acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta cggccctcag
    14941 ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga tgcctctgtc
    15001 gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact gttggttcac
    15061 cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt catttgcatg
    15121 ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg aattactgac
    15181 aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc caaatcagca
    15241 ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa aatttcattt
    15301 cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt actgggaata
    15361 gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga gataaaacta
    15421 gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc tttaaataat
    15481 ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat aactatagac
    15541 agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa cactctgctt
    15601 tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta acgttcagag
    15661 agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg gaagctgcac
    15721 gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg
    15781 gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa atgggcaaaa
    15841 aaatctgcgt tttgtttatc aactgggagg c
//
LOCUS       DAJTNP010000086        15607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTNP010000086 DAJTNP010000000
VERSION     DAJTNP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..72)
                     /locus_tag="OO160_003025"
     CDS             complement(<1..72)
                     /locus_tag="OO160_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5525057.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(170..1279)
                     /gene="anmK"
                     /locus_tag="OO160_003026"
     CDS             complement(170..1279)
                     /gene="anmK"
                     /locus_tag="OO160_003026"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5525058.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1553..2020
                     /gene="slyB"
                     /locus_tag="OO160_003027"
     CDS             1553..2020
                     /gene="slyB"
                     /locus_tag="OO160_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5525059.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2067..2501)
                     /gene="slyA"
                     /locus_tag="OO160_003028"
     CDS             complement(2067..2501)
                     /gene="slyA"
                     /locus_tag="OO160_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5525060.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2702..2938
                     /locus_tag="OO160_003029"
     CDS             2702..2938
                     /locus_tag="OO160_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5525061.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2941..3798
                     /locus_tag="OO160_003030"
     CDS             2941..3798
                     /locus_tag="OO160_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5525062.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3798..5810
                     /locus_tag="OO160_003031"
                     /pseudo
     CDS             3798..5810
                     /locus_tag="OO160_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5811..6332)
                     /gene="sodC"
                     /locus_tag="OO160_003032"
     CDS             complement(5811..6332)
                     /gene="sodC"
                     /locus_tag="OO160_003032"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5525063.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6413..7309)
                     /locus_tag="OO160_003033"
     CDS             complement(6413..7309)
                     /locus_tag="OO160_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5525064.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7358..7597)
                     /locus_tag="OO160_003034"
     CDS             complement(7358..7597)
                     /locus_tag="OO160_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5525065.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7700..8299
                     /gene="nemR"
                     /locus_tag="OO160_003035"
     CDS             7700..8299
                     /gene="nemR"
                     /locus_tag="OO160_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5525066.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8336..9433
                     /gene="nemA"
                     /locus_tag="OO160_003036"
     CDS             8336..9433
                     /gene="nemA"
                     /locus_tag="OO160_003036"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5525067.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9514..9921
                     /gene="gloA"
                     /locus_tag="OO160_003037"
     CDS             9514..9921
                     /gene="gloA"
                     /locus_tag="OO160_003037"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5525068.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10024..10671
                     /gene="rnt"
                     /locus_tag="OO160_003038"
     CDS             10024..10671
                     /gene="rnt"
                     /locus_tag="OO160_003038"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5525069.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10764..12878
                     /locus_tag="OO160_003039"
     CDS             10764..12878
                     /locus_tag="OO160_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5525070.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12888..14180
                     /locus_tag="OO160_003040"
     CDS             12888..14180
                     /locus_tag="OO160_003040"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525071.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15130..15222
                     /locus_tag="OO160_003041"
     CDS             15130..15222
                     /locus_tag="OO160_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5525072.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15266..>15607)
                     /locus_tag="OO160_003042"
     CDS             complement(15266..>15607)
                     /locus_tag="OO160_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5525073.1"
                     /translation="RAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQR
                     CACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLK
                     QEPTEMIQATA"
BASE COUNT         3577 a         4133 c         4098 g         3799 t
ORIGIN      
        1 cagttgatta aaggcgctac aaccggagag aaggaccggc aaaatgataa ttaacagttt
       61 tttcatagtc atatcccgaa gactttcctg gtccggaggg caaataccct ccttaatctt
      121 ccaagtgtaa cggcagacac gataagaaaa aattcagtta attctgatat cacgagttgg
      181 cggggaaaat agcccccagt accgtctcct ggcttgcgcc agtgacggaa ggcagatttc
      241 ctggtaatcc cgccagcgtc cgccaggcaa gccaggcgaa agccaatgct tccatgtcat
      301 cgccactaat gccaacggca tcggtggtgg tgacttctgt gcctagcagt aataccgcca
      361 gacgcgccat gagtagcgga ttacgactac ctccaccaca taccatcaat cgttcgcagc
      421 caccgctcaa caaaacttgt tcagaaatgg tcacggcggt gagttctgcc agtgtggcct
      481 gaacatcacg gggatcaaca cccggaaaat ggcgtaaatg gcgctccagc cagccgtagt
      541 taaagtattc gcgtccggtg cttttcggtg caggttgcga gaaatacggg tcgctgagca
      601 tattttgcag cagtgggaga ataactttac ccgcccgtgc ccactcggca tctttatcgt
      661 aaggtttacc tgcctgacgc cagatccagg catccatcag catgttacca ggaccggtat
      721 cgtagccgcc aaccagctgc ccaggaatga gcagtgacag attggcgatg ccgccaatat
      781 tgagcaccat tcgtcgctcg gttgggtgag ccagcaaggc atgatggaac gcaggtacca
      841 gcggtgcgcc ttgtcctccc aaggcaatat cgcgacggcg aaaatcaccg acaaccgtaa
      901 ttccggtgcg tgccacaatt tgattgttat cgccaatctg taaagtgtgt ggtgccacgc
      961 ccgtcggttc atgccagacg gtttgaccgt gacagccgat cgcaactata tctctcgcct
     1021 gcaggttttg ttccttaagc aaggcattga ccgcatcagc aaaaagtcgc ccgagttgag
     1081 tatcaagctg tccaaactgc gaaagtgtaa gctgctggcc ctggcaaata tccagtacag
     1141 cctgtttcag agacaccggg atcggccagc tcaagcttgc cagctgtgcg acccggtgtt
     1201 catcaattgt cgccaacaca acatcaacac catcaaggct ggtgcctgac ataacgccaa
     1261 taaagcggcc cgatttcata gttcatcctt tttcaatctg acgtttgcgc accactcaaa
     1321 cataaacttt tcgtgaatac catgcggaat gaccgatttt taccgttggt agtaaaacat
     1381 tatcttcaaa tcaataatca tcatgaatgt tttgtttata attggttgat cctactttca
     1441 ttatgatttg ctcatatttg gtagaacatg taaccatgga ttcacatatg ccatatactt
     1501 tgaccatgag ggatgcttgc gtggcgtttc atggtgaaca ggagattttt caatgattaa
     1561 acgcgtattg gttgtttcaa tggtaggtct gtctcttgtc ggttgtgtta ataacgacac
     1621 cctgtcaggg gatgtttata ccgcttctga agcgaaacaa gtacagaatg tcagctatgg
     1681 caccatcgtt aacgtacgtc cggtacagat tcagggcggt gatgattcca acgttatcgg
     1741 tgcaattggc ggtgctgttc ttggtggttt cctggggaat actgttggtg gcggaaccgg
     1801 gcgttctctg gctactgcag caggcgctgt tgcaggtggc gtagctggtc agggcgtaca
     1861 gagtgcaatg aacaaaacgc agggtgtcga gctggaaatt cgtaaagacg atggtaatac
     1921 catcatggtg gtacagaaac aaggcaacac tcgtttctct ccgggccaac gtgtcgtact
     1981 ggccagcaat ggcagtcagg tgaccgtttc tccgcgctaa ataagtttgc gtgtggtcag
     2041 gttactgacc acacgccccc ttcatttcac cctttggcct gtaactcaat gatattatgc
     2101 tcaagttttg cgatgagcgt aatcagttgc tccagttcct ctgcggagat gccatgtaat
     2161 atttccgcgc gggttttgtt aataacagct tccatttcgc tgatcagtgg ctctgccttt
     2221 tccgtcagtt taatacgttt agcccgacga tcgctggcac aagtttgacg cgaaattaac
     2281 cctttttctt ccagttggtc cagagtacgg accagtgatg gctgctcgat gccaatcgct
     2341 tttgccagtt gaatttgcga ctggtctgga ggtaactgat ggatattgtg taacgtaacc
     2401 caatgggttt gtgttaactc cagcggtttc aggcgatggt ctatcagagc acgccatatg
     2461 cgcaccaacc gtgccagatc agaacctagt ggcgattcca atttcatctc cttataatta
     2521 gcttgctaag atattatgcg gcttttagaa tagtgtgcag caattgtatt gctaaaacaa
     2581 atgtattgct gcatttggtt accgtcagac atatttttca gaaattgcgc gtaaattttt
     2641 cgcacttaaa gaatatttat taatctaacg caatatattc ggtcgtaaag gaatctactt
     2701 tgtgaagttt atgctcaatg caacaggatt gcccttacaa gatctggtgt tcggtgcgtc
     2761 cgtctatttt cctccgtttt tcaaagcatt cgcgtttgga ttcgtcatct ggcttgtcgt
     2821 acaccgcctg cttcgtggct ggatctacgc cggcgatatc tggcatccct tgttaatgga
     2881 tttatcgctg tttgcgattt gcgtttgcct tgctctggca atactgattg cgtggtaact
     2941 atgtcaatta aaacaattaa gtatttctca acaatcattg tagcgatagt tgcggttctt
     3001 gccggatggt ggctgtggaa ttattacatg caatctccgt ggacacgcga cgggaaaata
     3061 cgcgcagaac aggtttctat caccccacag gtgtcgggac gtattgttga gctgaatata
     3121 aaagacaacc aactggttaa cgcgggggaa cttctgctca cgatcgataa aacgcccttt
     3181 cagatcgccg agctgaacgc acaggctcag ctggcgaaag cgcaatctga cctcgcaaaa
     3241 gccaataacg aagcgaatcg tcgtcgtcat ctctcacaaa attttatttt tgccgaagaa
     3301 ctcgataccg ctaacctcaa tgttaaagcg atgcaggcca gtgttaatgc cgcacaggcg
     3361 acgctgaaac agacacaatg gcaactggcg caaacggaaa ttcgcgcccc ggtgagtgga
     3421 tgggtgacta acctcactac ccgcatcggc gattacgctg acaccgggaa accgctgttt
     3481 gcccttgtcg acagccactc gttttacgtc attggttatt ttgaagaaac caagttgcgc
     3541 catatccgcg aaggtgcacc agcacaaatt accctctata gtgataacaa aacgttacag
     3601 ggtcacgttt ccagtatcgg tcgggcgatt tatgatcaga gcgttgaaag tgactcaagc
     3661 cttattccgg atgtgaaacc taacgtcccc tgggttcgtc tcgcccaact cgttcccgtc
     3721 cgctttgcgc tggataaagt tccaggcgat gtcacgctgg tgtcgggcac cacctgtagc
     3781 attgccgtag gtcaataatg aacgcatcgt catggtcctt gcgcaatttg ccctggttca
     3841 gggccacgct ggcgcaatgg cgttatgcgt tacgcaatac cattgccatg tgtctggcgc
     3901 tgacggttgc ctattatttg aatctggatg aaccctattg ggcgatgacc tcggctgcag
     3961 tggttagctt tcccaccgtt ggcggtgtca tcagcaaaag cctcggacgc atggctggca
     4021 gtttactggg agccattgcg gcactgcttc ttgccggaca tacgctcaat gagccgtggt
     4081 tttttctatt gagcatgtcg gcgtggcttg gcttttgtac ctaggcctgt gcgcacttca
     4141 cgaataacgt cgcgtatgca tttcaactgg cgggctacac ggctgccatc atcgcctttc
     4201 cgatggttaa tattactgag gccagccagc tgtgggatat cgctcaggcg cgcgtttgcg
     4261 aggtgatagt cggtattttg tgcggcggca tgatgatgat gatcctgcct agcagttccg
     4321 atgctactgc ccttttaact gcattgaaaa acatgcacgc ccgattactg gaacatgcca
     4381 gtttactctg gcagcctgaa acaaacgatg ccattcgtgc agcacatgag ggggtgattg
     4441 ggcagatact gaccatgaat ttgctgcgta tccaggcttt ctggagccac tatcgttttc
     4501 gccagcaaaa cgcgcgcctt aatgcgctac tccaccagca attacgtatg accagtgtca
     4561 tctccagcct gcgacgtatg ttgctcaact ggccctcacc gccaggtgcc acgcgagaaa
     4621 ttctcgaaca attgctgacg gcgctcgcca gttcgcaaac agatgcttac accgtcgcac
     4681 gtattatcgc cccgctacgc ccgaccaacg tcgccgacta ccggcacgtc gccttctggc
     4741 agcgactacg ttatttttgc cgcctttatc tgcaaagtag tcaggaatta catcgtctgc
     4801 aaagcggtgt agatgatcat gccagactcg cacggacatc cgctctggct cgtcataccg
     4861 acaacgccga agctatgtgg agcgggctgc gtacattttg tacgttgatg atgattggcg
     4921 catggagtat tgcatcgcaa tgggatgccg gtgccaatgc attaacgctg gcagcaatta
     4981 gctgcgtact ctactccgcc gtcgcagcac cgtttaagtc gttgtcactg ctgatgcgca
     5041 cgctggtgtt actttcgcta ttcagctttg tggtcaaatt tggcctgatg gtccagatta
     5101 gcgatctgtg gcaattttta ctgtttctct ttccactgct ggcgacaatg cagcttctta
     5161 aattgcagat gcccaaattt gccgcactgt gggggcaact gattgttttt atgggttcgt
     5221 ttatcgctgt cactaatccc ccggtgtatg attttgctga ttttcttaac tataatctgg
     5281 caaaaatcgt tggcgttgcg ctggcttggc tagcgttcgc cattctgcgt ccaggatcgg
     5341 atgctcgtaa aagccgccgc catattcgcg cgctgcgccg ggattttgtc gatcagctaa
     5401 gccgccatcc aacactgagt gaaagcgaat ttgaatcgct cacttatcat cacgtcagtc
     5461 agttgagtaa cagccaggat gcgctggctc gccgttggtt attacgctgg ggtgtagtgc
     5521 tgctgaactg ttctcatgtt gtctggcaat tgcgcgactg ggaatcgcgt tccgatccgt
     5581 tatcgcgagt acgggataac tgtatttcac tgttgcgggg agtgatgagt gagcgtggcg
     5641 ttcagcaaaa atcactggcg gccacacttg aagaattaca gcggatttgc gacagccttg
     5701 cccgtcatca tcaacctgcc gcccgtgagc tggcggcaat tgtctggcgg ctgtactgct
     5761 cgctttcgca acttgagcaa gcaccaccgc aaggtacgct ggcctcgtaa ttacttaatt
     5821 acaccacagg catagcgttc accgccaccg cccagcggtt taggttgatc ggacatatta
     5881 tcgccgccaa cgtggaccat cagcgccttg tctttgattt catccagtga tttcagacga
     5941 ggcgcgatga cggcatcggt agctttgccg tcattattga cgaccagtgc aggcagatcg
     6001 cctaaatgcc cggtaccttc tggcccttca tgtttaccgg tattttgtgg atcaagatgc
     6061 ccgcctgcgg attccgcggc gctggctttg ccatctttgg tggctggctg gcagcttcct
     6121 ttggcatgaa tatggaagcc atgttcaccg gggggtaatg ctttcagatc gggtgaaaac
     6181 tccagacctt tatcggtttc agtaatggtg acgctaccaa ttgactgccc aaccccttgc
     6241 gacgtgacga ggttcatctc gactttttca ctggcagctt gtgcgccggt cgcaacaacc
     6301 agcgccagaa tggccagact aaaacgtttc ataggacctc cgttcattgt gcattcctga
     6361 ataagtgtaa accagtgaca catttttgaa cggcggctat tcctaaaagt gcttacggta
     6421 cgtcgtaccc cagtgcagct ttacggatac gaaaccattg ttgacgggtc attttcagtg
     6481 tttctgcttc gacagctgcc cgtacgcgct caattttacc tgagccgata attggcagcg
     6541 gctgcgacgg taaacgtaat acccaggcgt aaaccacctg ttcaatcgag cccgcgttta
     6601 actcctccgc caccacagcc agttcatcac gcagcggctg gaaataatca tcattaaaca
     6661 gacgaccacc accaaggcag gaccacgcca tcggacgaac acgcagttgt tgtagttggt
     6721 cgagcgtgcc atccagcagt aacggctgat gcaccgggga tatttccacc tgattagtgg
     6781 caagggtaaa cggcagacgt gattgcaaca gggcaaattg cgcaggcgta aagttcgata
     6841 cgccaaaatg acgcactttg ccgctctgat gcagatgttt gaacgcgtcc gccacttcat
     6901 cggcatccat taacgggtct ggtcggtgga ttaacagcag atccagatga tcggtcgcga
     6961 gattaattag cgactgttcg gcgctcttaa tgatgtgatt gcggtcagtg atgtaatgac
     7021 caatgacgtt ttcttcacgc gcggtcgtcg cgataccgca tttactgacg atttccatcc
     7081 gttcacgcag gtgaggtgcc agtttcagtg cctcgccaaa cgccgcttcg cactgatagc
     7141 caccataaat atcagcatgg tccacggtgg tcacgccgag atccagatgc tcttcaataa
     7201 aactgaccag ctggcgggcg gacatattcc agtccatcaa tcgccagtag cccatcacaa
     7261 aacgggaaaa ctccgggcct tccggcgcaa tagtaatacg ctgaaccata atcgcttcct
     7321 cttatcagat ataagaggag tatacgcaag attaggttca aaagagtgat ggttgctccg
     7381 gttcgtctga tgacgctggc ttatttgcgc gtaatttgcg cattaatcgc tgccgacaaa
     7441 ggcgcagcac ctcttgtttt tcgccatcgc tcattttatt ccagttaaaa cgctcatccc
     7501 gactacgaaa acagccgcga caaaacccgc gttcgtcaga ctggcaaatt ccccggcacg
     7561 ggctctggac gggaaagaac tctaattgct ccgccacctc gccctcctca gataagatta
     7621 ttaccattat tgaagctgtt aatgtccaaa gtagcaactt tgcttgcact agaccaactg
     7681 gtctactaca ctccaacgca tgaacaaaca caccgaacat gatactcgcg aacatctctt
     7741 ggcgacgggc gagcaacttt gcctgcaacg tggattcacc gggatggggc taagcgaatt
     7801 actaaaaacc gctgaagtgc cgaaagggtc cttctatcac tactttcgct ctaaagaagc
     7861 gtttggcgtt gccatgcttg agcgtcatta cgccgcatat caccagcgac tggctgagtt
     7921 gctgcaatcc ggcgaaggta actaccgcga ccgcatactg gcttattacc agcaaacact
     7981 gaaccagttt tgccaacatg gaaccatcag tggttgcctg acagtaaaac tctctgccga
     8041 agtgtgcgat ctgtcagaag atatgcgcag cgcgatggat aaaggtgctc gcggcgtgat
     8101 cgccctgctc tcgcaggctc tggaaaatgg ccgtgagaac cattgtttaa ccttttgtgg
     8161 cgaaccgcta caacaggcac aagtgcttta cgcactatgg cttggcgcga atctgcaggc
     8221 caaaatttcg cgcagtttcg agccactgga aaacgcgctg gcccatgtaa aaaacattat
     8281 tgcgacgcct gccgtttagc aggcattttt tatcaccaga cgaccgggag cctttatgtc
     8341 atctgaaaaa ctgtattccc cactgaaagt gggcgcgatc acggcggcaa accgtatttt
     8401 tatggcaccg ctgacgcgtc tgcgcagtat tgaaccgggt gacattccta ccccgttgat
     8461 ggcggaatac tatcgccaac gtgccagtgc cggtttgatt attagtgaag ccacgcaaat
     8521 ttctgcccag gcaaaaggat atgcaggtgc gcctggcatc catagtccgg agcaaattgc
     8581 cgcatggaaa aaaatcaccg ctggcgttca tgctgaaaat ggtcatatgg ccttgcagct
     8641 gtggcacacc ggacgcattt ctcacgccag cctgcaacct ggcggtcagg caccggtagc
     8701 gccttcagca cttagcgcgg gaacacgtac ttctctgcgc gatgaaaatg gtcaggcgat
     8761 ccgcgttgaa acatccatgc cgcgtgcgct tgaactggaa gagattccgg gtatcgtcaa
     8821 tgatttccgt caggccattg ctaacgcgcg tgaagccgct tttgatctgg tagagctcca
     8881 ctctgctcac ggttatttgc tgcatcagtt cctttcccct tcttcaaacc atcgtaccga
     8941 tcagtacggc ggcagcgtgg aaaatcgcgc acgtttggta ctggaagtgg tcgatgccgg
     9001 gattgaagaa tggggtgccg atcgcattgg cattcgcgtt tcgccaatcg gtactttcca
     9061 gaacacagat aacggcccga atgaagaagc cgatgcactg tatctgattg aacaactggg
     9121 taaacgcggc attgcttatc tgcatatgtc agaaccagat tgggcggggg gtgaaccgta
     9181 tactgatgcg ttccgcgaaa aagtacgcgc ccgtttccac ggtccgatta tcggcgcagg
     9241 tgcatacaca gtagaaaaag ctgaaacgct gatcggcaaa gggttaattg atgcggtggc
     9301 atttggtcgt gactggattg cgaacccgga tctggtcgcc cgcttgcagc tcaaagctga
     9361 gcttaaccca cagcgtgccg aaagtttcta cggtggcggc gcggaaggct ataccgatta
     9421 cccgacgttg taatccaaca ttgcgagcgg cgtaaagccg ccgctatact aaaacaacat
     9481 tttgaatctg ttagccattt tgaggataaa aagatgcgtc ttcttcatac catgctgcgc
     9541 gttggcgatt tgcaacgctc catcgatttt tataccaaag tgctgggcat gaaactgctg
     9601 cgtaccagcg aaaacccgga atacaaatac tcactggcgt ttgttggcta cggcccggaa
     9661 accgaagaag cggtgattga actgacctac aactggggcg tggataaata cgaactcggc
     9721 actgcttatg gtcacatcgc gcttagcgta gataacgccg ctgaagcgtg cgaaaaaatc
     9781 cgtcaaaacg ggggtaacgt gacccgtgaa gcgggtccgg taaaaggcgg tactacggtt
     9841 atcgcgtttg tggaagatcc ggacggttac aaaattgagt taatcgaaga gaaagacgcc
     9901 ggtcgcggtc taggcaacta atctcctgcc gggcgtgaac tcatcgcgcc cgcatcttta
     9961 ctgcatcgac aagtaatatt tgtcataatg cgcgctgcaa tttatccgta ttaagagaat
    10021 cagatgtccg ataacgctca acttaccggt ctgtgcgacc gttttcgtgg tttttatcct
    10081 gttgtgatcg atgttgaaac agccggattt aacgccaaaa ccgatgcgct gcttgagatt
    10141 gccgccatca ccctgaaaat ggatgaacaa ggctggctga tgccggacac cacattacat
    10201 ttccacgtcg aaccatttgt cggcgcaaat ttgcaaccag aagccctcgc cttcaacggc
    10261 attgacccga acgatcccga tcgcggcgcg gtcagcgaat acgaggcgct gcacgaaatt
    10321 tttaaagttg tacgtaaagg tattaaagcg agcggctgta accgcgccat tatggtggcg
    10381 cacaacgcca attttgatca cagctttatg atggccgccg cagaacgcgc ctcgctgaaa
    10441 cgtaacccgt tccacccttt cgccactttt gacactgctg cactggccgg gctggcactc
    10501 ggacaaaccg tattgtcaaa ggcttgccag accgctggca tggacttcga cagcacccag
    10561 gcgcactccg cgctgtacga caccgaacgc actgctgtgc tgttttgtga aatcgtcaac
    10621 cgctggaaac gtctgggagg ctggccgcta cctgccgccg aagaggtgta atcgagtcga
    10681 tgcctgatga catgcaatga ttcaggcatc tatagtgagg ctattccacg catcaagcat
    10741 gatattcacg gggaatagcg ttaatggcag ataatccaga cccttcatcg ctcctgccgg
    10801 acgtgttttc accggcgacc cgcgactggt ttcttcgcgc ctttaaacag ccgaccgctg
    10861 tccagtcgca aacctggcat gtggcggcgc gaggcgaaca tgcgctggtg attgcaccga
    10921 ctggctccgg gaaaacgctg gcagcatttc tctacgccct cgatcggctc ttccgcgaag
    10981 gcggcgaaga tacccgcgag gcgcataagc gtaaaacctc acgcatcctc tatatttcac
    11041 cgataaaagc cctgggcacc gacgttcagc gcaacttgca gatcccgttg aagggtattg
    11101 ccgatgaacg gcggcggcgc ggcgaaacgg taattattga tgaagtccac gcggtagcgg
    11161 gcagtaaacg tggtgcgcat ctggcgttaa gtctggagcg gctcgatgcg ctgctccaca
    11221 cctcagcaca gcgaattggc ctttctgcca ctgtgcgctc agccagcgat gtggcagcat
    11281 ttcttggtgg cgatcgcccg gttacggtag tcaacccgcc cacaatgcgc catccgcaga
    11341 tacgaattgt cgtaccggtc gccaatatgg atgatgtctc atcggtcgcc agcggcaccg
    11401 gcgaagacag ccatgccggc cgggaaggct ccatctggcc atatattgaa acgggtatcc
    11461 ttgatgaagt gttgcgccat cgctcgacca ttgtctttac taattcacgc gggctggcgg
    11521 aaaaactgac ggcacgatta aatgagcttt acgccgcacg cttacagcgt tccccgtcta
    11581 tcgccgttga tgcggcccat ttcgagtcga cctccggcgc aacctctaac cgtgtacaaa
    11641 gtagcgacgt ttttattgcc cgctcacacc acggctccgt ctctaaagaa caacgagcaa
    11701 tcaccgaaca ggcgctgaaa tcgggtgaat tacgctgcgt ggtcgcaacc tccagtcttg
    11761 aactggggat tgatatgggc gcggtggatc tggtgattca ggtggcaacg ccgctttctg
    11821 ttgccagtgg gttacaacgc attggtcgcg ccggacatca ggttggcggt gtatctaaag
    11881 ggctgttttt cccccgtacc cggcgtgatt tagtcgattc cgcagtcatt gtagagtgta
    11941 tgttcgcagg caggctggaa aacctgacac caccgcataa tcctctcgac gtccttgcgc
    12001 agcaaaccgt tgccgccgcg gcgatggatg cattacaggt agacgaatgg tactcccgcg
    12061 tacgccgtac cgcaccgtgg aaagatctgc caagacgtgt ttttgacgcc acgctggata
    12121 tgctttccgg gcgctatccc tctggcgatt tttctgcttt tcgccccaaa ctggtctgga
    12181 acagggagac cgggatattg accgcccgac ctggcgctca attgttggcg gttaccagcg
    12241 gcggcaccat tccggatcgt ggcatgtata gcgtgttatt acccgaaggt gaagaaaagg
    12301 ccggttcgcg gcgggtgggt gaactggatg aggagatggt atatgagtcg cgggtgaacg
    12361 acattatcac tctcggcgct acctcatggc ggatccagca aatcacccgc gatcaggtga
    12421 ttgtgactcc tgctccgggt cgttctgccc agctcccctt ctggcgtggt gaaggtaacg
    12481 gacgtccggc tgaattaggc gagatgatcg gcgattttct tcatttactg gcggatggcg
    12541 cgttcttttc cgggactatt cccccgtggc tggcagaaga aaatacgaac gccaatattc
    12601 aggggttgat tgacgagcaa cgcaacgcga cgggcatcgt tccggggagt cgccatctgg
    12661 tcctcgaacg gtgccgtgat gaaattggcg actggcgtat tattttgcac tctccctatg
    12721 gaagacgggt gcatgaaccc tgggcgctgg cgattgccgg gcgaatacat gccctatggg
    12781 gcgctgacgc gtctgtggtc gccagtgatg acagcattgt tgcgcgcatt cctgacactg
    12841 atggtaaatt gcccgacgcc gcgatttttt tgttttaacc agaaaagttg ctgcaaattg
    12901 tccgcgaggc ggtaggcagc tcggcacttt tcgccgcccg ttttcgcgaa tgcgccgcgc
    12961 gggcattatt aatgccgggc cgcacgccgg gccatcgcac cccgctttgg caacaacggc
    13021 tgcgcgccag tcagttgctg gaaatcgctc agggatatcc ggattttccg gtcattctcg
    13081 aaaccctacg cgaatgtctg caagatgttt atgatcttcc cgcactggaa cgtttgatgc
    13141 gtcgcctgaa cggtggcgaa attcaaatat ccgatgtaac gaccactacg ccctcgcctt
    13201 tcgccacaag tttattgttc ggctatgtcg cggaatttat gtaccagagc gacgccccgc
    13261 tggcagagcg ccgggcatcc gtactgtcgc tggacagcga gttactgcgc aatctactcg
    13321 gacaggtcga tccgggggaa ttactcgacc cgcaggtcat tcgccaggtg gaagaagagt
    13381 tgcaacgact ggctcctggt agaagagcga aaggtgaaga aggattgttc gacctgctgc
    13441 gcgaactggg gccaatgacc gttgaagacc tggcgcaacg gcatacaggc agcagtgaag
    13501 aggttgcgtc gtatctggaa aatcttcttg cagtaaaacg aatcttccca gcgatgatta
    13561 gcggacagga gcgtcttgcc tgtatggatg atgccgccag gctgcgtgat gccctcggcg
    13621 tacggctacc agagtcattg ccagagattt atttacatag agtcagttac ccgcttcgcg
    13681 acctctttct gcgctatctc cgggctcatg ctctggtcac ggctgaacaa ctggctcatg
    13741 agtttagtct cggtattgcc attgtcgaag agcagcttca gcaactgcgt gaacagggtc
    13801 tggtgatgaa tctgcaacaa gacatctggg tgagcgatga agtatttcgt cgtctgcgtt
    13861 tgcgctcgct gcaagccgcc agagaagcga cgcgtcccgt tgcagccacg acctatgcgc
    13921 gattgctgct ggaacgtcag ggcgtattac ccgccaccga tggtagcccg gcgctctttg
    13981 cctcaacatc tccaggcgtt tatgagggcg tagatggagt gatgcgggtg atcgaacagc
    14041 ttgccggagt cggtttaccc gcctcactct gggaaagcca gatcctgcct gcccgcgtac
    14101 gcgactattc gccagaaatg ctcgatgaat tactggcaac cggagcggtt atctggtcgg
    14161 gacaaaaaaa agctgggtga agatgacggc ctggtggcac tgcatctaca gaaatatgcc
    14221 gcagaatcgt tcactcccgc cgaagcggat caggcgaatc gttcggcgct gcagcaagcg
    14281 ataatcgctg ttctggctga cggaggagcc tggtttgcac aacaaatcag ccaacggata
    14341 cgcgataaaa tcgtcgaatc ggttgattcc tcggcactgc aagaggcgtt atgggcgctg
    14401 gtctggcaag gcgtcattac cagcgatatt tgggcaccgt tacgcgccct cacccgcagc
    14461 agttccaacg cacgcacctc aactcgccgc agtcaccggg ctcgtcgtgg gcgtcctgtc
    14521 tatgcacaac ccgtctcgcc gcgggtatct tacaacacac caaatctggc tggacgctgg
    14581 tcgttattgc aggtggagcc actaaacgat accgaaagga tgctggcgct ggcggaaaat
    14641 atgctcgacc gctacggcat catcagtcgt caggcggtga tagccgaaaa tatccctggc
    14701 gggtttccat cgatgcaaac actttgtcga agtatggaag actccgggcg aattatgcga
    14761 ggtcgttttg tagaaggtct gggtggcgcg caattcgctg aacgtctgac tattgaccga
    14821 ttgcgcgatc tggcgacaca agccacgcaa acacgccact atacaccagt ggcgctctcc
    14881 gccaacgatc cggctaatgt gtggggaaat cttctgccct gggctgcaca tccggcaacg
    14941 ctggtaccaa cgcgtcgggc gggtgcgctg gtggtcgttt ctggcggcaa attgttactc
    15001 tatctggcgc aaggcggcaa aaaaatgctg gtctggcagg aaaaggagga attactcgcc
    15061 ccagagattt tccaggcgct gaccaccgca ctgcgtcgcg aaccaaggct gcgctttacg
    15121 ctaacagaag tgaatgatct tccggtccgg caaacgccga tgtttacgct gctgcgtgag
    15181 gcgggatttt caagttcgcc acaagggctg gattggggat agttgacatc ctccacgccc
    15241 tgaaagacgt ggttccctgc tacgttcaag ctgtcgcctg aatcatttcg gtgggttcct
    15301 gcttcaacga gcggcctgac tgcaccatct ctccacaggc aagaacggcg tgccccgccg
    15361 ctaaaatgtt acgagcgcca tttacatcgg cgttcgctgt atatccacat acctggcatc
    15421 tgaatttact ttgtgacagg cggttttctt tcgctgtatg accacagcac gcgcaacgct
    15481 ggcttgtgta cgctggcgga acagcaagca cctgaccgcc actccagagc tgcttgtact
    15541 caagctggcg gcgcatttca taccagccct gatccagtat cgaacggttt aaacctgatt
    15601 ttgcccg
//
LOCUS       DAJTNP010000087        15479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTNP010000087 DAJTNP010000000
VERSION     DAJTNP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..69
                     /locus_tag="OO160_003043"
     CDS             <1..69
                     /locus_tag="OO160_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525074.1"
                     /translation="LRFAIREGGRTVGAGVVAKVLG"
     gene            252..446
                     /gene="bfd"
                     /locus_tag="OO160_003044"
     CDS             252..446
                     /gene="bfd"
                     /locus_tag="OO160_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5525075.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            519..995
                     /gene="bfr"
                     /locus_tag="OO160_003045"
     CDS             519..995
                     /gene="bfr"
                     /locus_tag="OO160_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5525076.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(992..1459)
                     /locus_tag="OO160_003046"
                     /pseudo
     CDS             complement(992..1459)
                     /locus_tag="OO160_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1838..2149
                     /gene="rpsJ"
                     /locus_tag="OO160_003047"
     CDS             1838..2149
                     /gene="rpsJ"
                     /locus_tag="OO160_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5525077.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2182..2811
                     /gene="rplC"
                     /locus_tag="OO160_003048"
     CDS             2182..2811
                     /gene="rplC"
                     /locus_tag="OO160_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5525078.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2822..3427
                     /gene="rplD"
                     /locus_tag="OO160_003049"
     CDS             2822..3427
                     /gene="rplD"
                     /locus_tag="OO160_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5525079.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3424..3726
                     /gene="rplW"
                     /locus_tag="OO160_003050"
     CDS             3424..3726
                     /gene="rplW"
                     /locus_tag="OO160_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5525080.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3744..4565
                     /gene="rplB"
                     /locus_tag="OO160_003051"
     CDS             3744..4565
                     /gene="rplB"
                     /locus_tag="OO160_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5525081.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4582..4860
                     /gene="rpsS"
                     /locus_tag="OO160_003052"
     CDS             4582..4860
                     /gene="rpsS"
                     /locus_tag="OO160_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5525082.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4875..5207
                     /gene="rplV"
                     /locus_tag="OO160_003053"
     CDS             4875..5207
                     /gene="rplV"
                     /locus_tag="OO160_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5525083.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5225..5926
                     /gene="rpsC"
                     /locus_tag="OO160_003054"
     CDS             5225..5926
                     /gene="rpsC"
                     /locus_tag="OO160_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5525084.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5939..6349
                     /gene="rplP"
                     /locus_tag="OO160_003055"
     CDS             5939..6349
                     /gene="rplP"
                     /locus_tag="OO160_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5525085.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6349..6540
                     /gene="rpmC"
                     /locus_tag="OO160_003056"
     CDS             6349..6540
                     /gene="rpmC"
                     /locus_tag="OO160_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5525086.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6540..6794
                     /gene="rpsQ"
                     /locus_tag="OO160_003057"
     CDS             6540..6794
                     /gene="rpsQ"
                     /locus_tag="OO160_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5525087.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6959..7330
                     /gene="rplN"
                     /locus_tag="OO160_003058"
     CDS             6959..7330
                     /gene="rplN"
                     /locus_tag="OO160_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5525088.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7341..7655
                     /gene="rplX"
                     /locus_tag="OO160_003059"
     CDS             7341..7655
                     /gene="rplX"
                     /locus_tag="OO160_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5525089.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7670..8209
                     /gene="rplE"
                     /locus_tag="OO160_003060"
     CDS             7670..8209
                     /gene="rplE"
                     /locus_tag="OO160_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5525090.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8224..8529
                     /gene="rpsN"
                     /locus_tag="OO160_003061"
     CDS             8224..8529
                     /gene="rpsN"
                     /locus_tag="OO160_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5525091.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8563..8955
                     /gene="rpsH"
                     /locus_tag="OO160_003062"
     CDS             8563..8955
                     /gene="rpsH"
                     /locus_tag="OO160_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5525092.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8968..9501
                     /gene="rplF"
                     /locus_tag="OO160_003063"
     CDS             8968..9501
                     /gene="rplF"
                     /locus_tag="OO160_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5525093.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9511..9864
                     /gene="rplR"
                     /locus_tag="OO160_003064"
     CDS             9511..9864
                     /gene="rplR"
                     /locus_tag="OO160_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5525094.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9879..10382
                     /gene="rpsE"
                     /locus_tag="OO160_003065"
     CDS             9879..10382
                     /gene="rpsE"
                     /locus_tag="OO160_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5525095.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10386..10565
                     /gene="rpmD"
                     /locus_tag="OO160_003066"
     CDS             10386..10565
                     /gene="rpmD"
                     /locus_tag="OO160_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5525096.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10569..11003
                     /gene="rplO"
                     /locus_tag="OO160_003067"
     CDS             10569..11003
                     /gene="rplO"
                     /locus_tag="OO160_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5525097.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11011..12342
                     /gene="secY"
                     /locus_tag="OO160_003068"
     CDS             11011..12342
                     /gene="secY"
                     /locus_tag="OO160_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5525098.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12374..12490
                     /gene="rpmJ"
                     /locus_tag="OO160_003069"
     CDS             12374..12490
                     /gene="rpmJ"
                     /locus_tag="OO160_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5525099.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12637..12993
                     /gene="rpsM"
                     /locus_tag="OO160_003070"
     CDS             12637..12993
                     /gene="rpsM"
                     /locus_tag="OO160_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5525100.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13010..13399
                     /gene="rpsK"
                     /locus_tag="OO160_003071"
     CDS             13010..13399
                     /gene="rpsK"
                     /locus_tag="OO160_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5525101.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13433..14053
                     /gene="rpsD"
                     /locus_tag="OO160_003072"
     CDS             13433..14053
                     /gene="rpsD"
                     /locus_tag="OO160_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5525102.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14079..15068
                     /gene="rpoA"
                     /locus_tag="OO160_003073"
     CDS             14079..15068
                     /gene="rpoA"
                     /locus_tag="OO160_003073"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5525103.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15109..>15479
                     /gene="rplQ"
                     /locus_tag="OO160_003074"
     CDS             15109..>15479
                     /gene="rplQ"
                     /locus_tag="OO160_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5525104.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEA"
BASE COUNT         3986 a         3632 c         4132 g         3729 t
ORIGIN      
        1 ctgcgtttcg caatccgtga aggcggccgt accgttggcg cgggcgttgt tgctaaagtt
       61 ctgggctaat tacacgttaa ttagttttga attgaaaagg gcgcttcggc gccctttttg
      121 ctgcccgtaa tttgagctcg aaatatttag tcgtaatttt gctcgctgaa ctatggcgca
      181 aataaaactg tgctattgta atcataacca ttctcattta cactttgtgc agaatttgaa
      241 cgggagcaat catgtacgtt tgtttgtgta atggtgtaag tgacaaaaaa attcgccagg
      301 cagtccgtca gtttcatccg caatctttcc aacaattgcg taagtttatt cctgtgggaa
      361 atcaatgcgg taagtgcatt cgcgccgcgc gcgaagtgat gcaggacgag ttgatgcaga
      421 tgccagaatt taaagagatt gcctgaagcc cacgcttatt ttttgacatc ctgctagccc
      481 catctacgct ctaatagtgg aagcggaggg actataaaat gaaaggtgat gttaaaatca
      541 taaattatct caataaacta ttgggaaatg agcttgtcgc aatcaatcag tattttctcc
      601 atgcccggat gtttaaaaac tggggcctga tgcgtctcaa tgacgtcgaa taccatgaat
      661 ccatcgatga gatgaaacac gctgataaat atatcgagcg tattttgttt ttagaaggaa
      721 tccccaactt acaggattta ggcaaactgg ggattggtga agatgtcgag gagatgctgc
      781 aatctgacct gcgattagaa ctggaaggcg cgaaggatct gcgtgaggct atcgcatatg
      841 ccgacagcgt tcatgactat gttagccgcg atatgatgat cgagatcctt gctgatgaag
      901 aaggtcatat cgactggctc gaaactgaac tggatctcat cggtaaaatc ggcctacaaa
      961 attacctgca atcacaaatt aaagttaaag attaacggcc tatcagcaaa ctatcccagc
     1021 ctataacgaa acccgcagcc gccagaaatg gcccaaaagg cagcgggttt tttaatgatt
     1081 cttttcctct catgagcaga ccaataacta cggctccgca agcgaatgag gcagcaagga
     1141 aaaccagtcg tggtaagaag gcccatgaat gctaggcgcc aagggcggcg agaaacttca
     1201 catcaccata gcctaatcct tctttatgtc gcagtatgcg atacccccag taaatgacag
     1261 cgaatgtgcc gtaaccaaca attgcccccc ataacgcatc ggctaaacca tcaggatgac
     1321 aaacttgata gaacaaaaga cctgaccaga gtaacgggca cgtaaatctg tcaggtaaca
     1381 agccatgctt tgcatcccag aaaaaaagta gggctgataa acaagcgtac aaaattagaa
     1441 agggaagggt agtgtccata aaacatcctc tgtaattgga gtatcaccat cgaataactc
     1501 cggatgctca gcaacagaga gagcgctgat ttgttaacct gattccggtg aaaaacgcta
     1561 tcttcacaac cttacaaatt tcgtggaatg cagctcgcaa tctgcgcgtt gcgattaatt
     1621 tctacccgat gcttgcgtca ggctcagatt tgcgtataat gcgcgggctt gtcgtagttg
     1681 acagcaggtt caatctgaac ctcagttagc cgaatttggc tacctaacaa tgctcccaat
     1741 cggggagcta cgtaagaacg gttacactct cccatcaatc gtaatgggtc tgaggagtaa
     1801 tcattttcgt ttataaaata attggagctc tggtctcatg cagaaccaaa gaatccgtat
     1861 ccgcctgaaa gcgtttgatc atcgtctgat cgatcaagca accgcggaaa tcgtcgagac
     1921 tgccaagcgc actggtgcgc aggtccgtgg tccgatcccg ctgccgacac gcaaagagcg
     1981 cttcactgtt ctgatctccc cgcacgtcaa caaagacgcg cgcgatcagt acgaaatccg
     2041 tactcacttg cgtctggttg acatcgttga gccaaccgag aaaaccgttg atgctctgat
     2101 gcgtctggat ctggctgccg gtgtagacgt gcagatcagc ctgggttaat caggtcattg
     2161 agcgattgag aggttgaaac aatgattggt ttagtcggta aaaaagtggg tatgacccgt
     2221 atcttcacag aagacggcgt ttctatccca gtaaccgtaa tcgaagttga agcaaaccgc
     2281 gttactcagg ttaaagacct ggctaacgat ggctaccgtg ctattcaggt gaccaccggt
     2341 gctaaaaaag ctaaccgtgt gaccaagcct gaagctggcc acttcgctaa agctggcgta
     2401 gaagctggcc gtggtctgtg ggaattccgc ctggctgaag gcgaagagtt cactgtaggt
     2461 cagagcatta gcgttgaact gtttgctgac gttaaaaaag ttgacgtaac tggcacctct
     2521 aaaggtaaag gtttcgcagg taccgttaag cgctggaact tccgtaccca ggacgctact
     2581 cacggtaact ccttgtctca ccgcgttccg ggttctatcg gtcagaacca gactccgggc
     2641 aaagtgttca aaggcaagaa aatggcaggt cagatgggta acgaacgtgt aaccgttcag
     2701 agccttgacg tagtacgcgt tgacgctgag cgcaacctgc tgctggttaa aggtgctgtc
     2761 ccgggtgcaa ccggtagcga cctgatcgtt aaaccagctg tgaaggcgta aggagatagc
     2821 aatggaatta gtattgaaag acgcgcagag cgcgctgact gtttccgaaa ctaccttcgg
     2881 tcgtgatttc aacgaagcgc tggttcacca ggttgttgtt gcttatgcag ctggtgctcg
     2941 tcagggtact cgtgctcaga agactcgtgc tgaagtaact ggttccggta aaaaaccgtg
     3001 gcgccagaaa ggcaccggcc gtgcgcgttc tggttctatc aagagcccga tctggcgttc
     3061 tggtggcgtg acctttgctg ctcgtccgca ggaccacagt caaaaagtta acaagaagat
     3121 gtaccgcggc gcgctgaaaa gcatcctgtc cgaactggta cgtcaggatc gtctgatcgt
     3181 tgtcgagaag ttctctgtag aagcgccgaa aactaagctg ctggcacaga aactgaaaga
     3241 catggctctg gaagatgtgc tgatcatcac cggtgagctg gacgaaaacc tgttcctggc
     3301 tgcgcgcaac ctgcagaagg ttgacgtacg cgatgcaact ggtatcgacc cggttagcct
     3361 gatcgccttc gacaaagtcg taatgactgc tgatgctgtt aagcaagttg aggagatgct
     3421 ggcatgattc gtgaagaacg tctgctgaag gtgctgcgtg caccgcacgt ttctgaaaaa
     3481 gcgtctactg cgatggaaaa atccaacacc atcgtactca aagttgctaa agacgcgacc
     3541 aaagcagaaa tcaaagctgc tgtgcagaaa ctgtttgaag tcgaagtcga agtcgttaac
     3601 accctggtag ttaaagggaa agttaaacgt cacggacagc gtatcggtcg tcgtagcgac
     3661 tggaaaaaag cttacgtcac cctgaaagaa ggccagaatc tggacttcgt tggcggcgct
     3721 gagtaagtcg gaggagtaat acaatggcag ttgttaaatg taaaccgaca tctccgggtc
     3781 gtcgccacgt agttaaagtg gttaaccctg agctgcacaa gggcaaacct tttgctccgt
     3841 tgctggaaaa aaacagcaaa tccggtggtc gtaacaacaa tggccgtatc accactcgtc
     3901 atatcggtgg tggccacaag caggcttacc gtattgttga cttcaaacgc aacaaagacg
     3961 gtatcccggc agttgttgaa cgtcttgagt acgatccgaa ccgttccgcg aacatcgcgc
     4021 tggttctgta caaagacggt gaacgccgtt acatcctggc ccctaaaggc ctgaaagctg
     4081 gcgaccagat tcagtctggc gttgatgctg caatcaaacc aggtaacacc ctgccgatgc
     4141 gcaacatccc ggttggttct actgttcata acgtagaaat gaaaccaggt aaaggcggtc
     4201 agctggcacg ttccgctggt acttacgttc agatcgttgc tcgtgatggt gcttatgtca
     4261 ccctgcgtct gcgttctggt gaaatgcgta aagtagaagc agactgccgt gcaactctgg
     4321 gcgaagttgg caatgctgag catatgctgc gcgttctggg taaagcaggt gctgcacgct
     4381 ggcgtggtgt tcgtccgacc gttcgcggta ccgcgatgaa cccggtagac cacccacatg
     4441 gtggtggtga aggtcgtaac tttggtaagc acccggtaac tccgtggggc gttcagacca
     4501 aaggtaagaa gacccgcagc aacaagcgta ctgataaatt catcgtacgt cgccgtagca
     4561 aataatttta gaggataagc catgccacgt tctctcaaga aaggtccttt tattgacctg
     4621 cacttgctga agaaggtaga gaaagcggtg gaaagcggag acaagaagcc cctgcgcact
     4681 tggtcccgtc gttcaacgat ctttcctaac atgatcggtt tgaccatcgc tgtccataat
     4741 ggtcgtcagc acgttccggt atttgtaacc gacgaaatgg tcggtcacaa actgggtgaa
     4801 ttcgcaccga ctcgtactta tcgcggccac gctgctgata aaaaagcgaa gaagaaataa
     4861 ggtaggagga agagatggaa actattgcta aacatcgcca tgctcgttct tctgctcaga
     4921 aggttcgcct tgttgctgac ctgattcgcg gtaagaaagt gtcgcaggct ctggatattt
     4981 tgacctacac caacaagaaa gcggctgtac aggtcaagaa agttctggaa tctgccattg
     5041 ctaacgctga acacaacgat ggcgctgaca ttgacgatct gaaagttacg aaaattttcg
     5101 tagacgaagg cccgagcatg aagcgcatta tgccgcgtgc aaaaggtcgt gcagatcgca
     5161 tcctgaagcg caccagccac atcactgtgg ttgtgtccga tcgctgagac tctggagact
     5221 agcaatgggt cagaaagtac atcctaatgg tattcgcttg ggtattgtaa aaccatggaa
     5281 ctctacttgg tttgcgaaca ccaaagaatt cgctgacaac ctggacagcg attttaaagt
     5341 acgtcagtac ctgactaagg aactggctaa agcgtccgta tctcgtatcg ttatcgagcg
     5401 tccggctaag agcatccgtg taaccattca cactgctcgc ccgggtatcg ttatcggtaa
     5461 aaaaggtgaa gacgtagaaa aactgcgtaa ggtcgtagcg gacatcgctg gcgttcctgc
     5521 acagatcaac atcgccgaag ttcgtaagcc tgaactggac gcaaaactgg ttgctgacag
     5581 catcacttct cagctggaac gtcgcgttat gttccgtcgt gctatgaagc gtgctgtaca
     5641 gaacgcaatg cgtctgggcg ctaaaggtat taaagttgaa gttagcggcc gtctgggcgg
     5701 cgcggaaatc gcacgtaccg aatggtaccg cgaaggtcgc gtaccgctgc acactctgcg
     5761 tgctgacatc gactacaaca cctctgaagc gcacaccact tacggtgtaa tcggcgttaa
     5821 agtgtggatc ttcaaaggcg agatcctggg tggtatggct gctgttgaac aaccggaaaa
     5881 accggctgct cagcctaaaa agcagcagcg taaaggccgt aaataaggag cgtcgctgat
     5941 gttacaacca aagcgtacaa aattccgtaa aatgcacaaa ggccgtaacc gcggtctggc
     6001 gcagggtacg gatgttagct tcggcagctt cggtctgaaa gctgttggcc gtggtcgtct
     6061 gactgcccgt cagatcgaag cagcacgtcg tgctatgacc cgtgcagtta agcgtcaagg
     6121 taagatctgg atccgtgtgt tcccggacaa accgatcact gaaaagccgc tggcagtgcg
     6181 tatgggtaaa ggtaaaggta acgtggagta ttgggttgcc ttgattcagc cgggtaaagt
     6241 cctgtatgaa atggacggtg ttccggaaga gctggcccgt gaagcattca agctggcagc
     6301 agcgaaactg ccgattaaaa ccacctttgt aactaagacg gtgatgtaat gaaagcaaaa
     6361 gagctgcgtg agaagagcat tgaagagctg aacaccgagc tgctgaacct gctgcgtgag
     6421 cagttcaacc tgcgtatgca ggctgcaagt ggccagctgc aacagtctca cctgttgaag
     6481 caagtgcgtc gcgatgtcgc acgcgttaag actttactga acgagaaggc gggtgcgtaa
     6541 tgaccgataa aatccgtact ctgcaaggtc gcgttgttag cgacaaaatg gagaaatcca
     6601 ttgttgttgc tatcgaacgt tttgtgaaac acccgatcta cggaaaattc atcaagcgta
     6661 cgaccaaact gcacgtacat gacgagaaca acgaatgcgg tatcggtgac gtggttgaaa
     6721 tccgcgaatg ccgtccgctg tccaagacta aatcctggac gctggttcgc gttgtagaga
     6781 aagcggttct gtaatacagt acactctctc gatacgaata aacggctcag aaatgagccg
     6841 tttatttttt ctacccatat ccttgaagcg gtgttataat gccgcgccct cgatatgggg
     6901 atttttaacg acctgatttt cgggtctcag tagtagttga cattagcgga gcactaaaat
     6961 gatccaagaa cagactatgc tgaacgtcgc cgacaactcc ggtgcacgtc gcgtaatgtg
     7021 tatcaaggtt ctgggtggct cgcaccgtcg ctacgcaggc gtaggcgaca tcatcaagat
     7081 caccatcaaa gaagcaattc cgcgtggtaa ggtcaaaaaa ggtgatgtgc tgaaggcggt
     7141 agtggtgcgc accaagaagg gtgttcgtcg cccggacggt tctgtcattc gcttcgatgg
     7201 taatgcttgt gttcttctga acaacaacag cgagcagcct atcggtacgc gtatttttgg
     7261 gccggtaact cgtgagcttc gtagtgagaa gttcatgaaa attatctctc tggcaccaga
     7321 agtactctaa ggagcgaatc atggcagcga aaatccgtcg tgatgacgaa gttatcgtgt
     7381 taaccggtaa agataaaggt aaacgcggta aagttaagaa tgtcctgtct tccggcaagg
     7441 tcattattga aggtatcaac ctggttaaga aacatcagaa gccggttccg gccctgaacc
     7501 aaccgggtgg catcgttgaa aaagaagccg ctattcaggt ttccaacgta gcaatcttca
     7561 atgcggcaac cggcaaggct gaccgtgtag gctttagatt cgaagacggc aaaaaagtcc
     7621 gtttcttcaa gtctaacagc gaaactatca agtaatttgg agtagtacga tggcgaaact
     7681 gcatgattac tacaaagacg aagtagttaa aaaactcatg actgagttta actacaattc
     7741 tgtcatgcaa gtccctcggg tcgagaagat caccctgaac atgggtgttg gtgaagcgat
     7801 cgctgacaaa aaactgctgg ataacgcagc agcagacctg gcagcaatct ccggtcaaaa
     7861 accgctgatc accaaagcac gcaaatctgt tgcaggcttc aaaatccgtc agggctatcc
     7921 gatcggctgt aaagtaactc tgcgtggcga acgcatgtgg gagttctttg agcgcctgat
     7981 cactattgct gtacctcgta tccgtgactt ccgtggcctg tccgctaagt ctttcgacgg
     8041 tcgtggtaac tacagcatgg gtgtccgtga gcagatcatc ttcccagaaa tcgactacga
     8101 taaagtcgac cgcgttcgtg gtttggatat taccattacc actactgcga aatctgacga
     8161 agaaggccgc gctctgctgg ctgcctttga cttcccgttc cgcaagtaag gtagggttac
     8221 taaatggcta agcaatcaat gaaagcacgc gaagtaaaac gcgtagcttt agctgataaa
     8281 tacttcgcga aacgcgctga actgaaagcg atcatctctg atgtgaacgc ttccgacgaa
     8341 gatcgttgga acgctgttct caagctgcag actctgccgc gtgattccag cccgtctcgt
     8401 cagcgtaacc gctgccgtca aacaggtcgt ccgcatggtt tcctgcggaa gttcgggttg
     8461 agccgtatta aggtccgtga agccgctatg cgcggtgaaa tcccgggtct gaaaaaggct
     8521 agctggtaat tgtcaccaat tgaatcacgg gagtaaagac agatgagcat gcaagatccg
     8581 atcgcggata tgctgacccg tatccgtaac ggtcaggccg cgaacaaagc tgcggtcacc
     8641 atgccttcct ccaagctgaa agtggcaatc gccaacgtgc tgaaggaaga aggttttatt
     8701 gaagatttta aagttgaagg cgacaccaag cctgaactgg aacttactct gaagtatttc
     8761 cagggcaaag ctgttgtaga aagcattcag cgtgtcagcc gcccaggtct gcgcatctat
     8821 aaacgtaaag atgagctgcc gaaagttatg gcgggtctgg gtatcgcagt tgtttctacc
     8881 tctaaaggtg ttatgactga tcgtgcagcg cgccaggctg gtcttggtgg cgaaattatc
     8941 tgctacgtag cctaatcgga ggaaaaaatg tctcgtgttg ctaaagcacc ggtcgttgtt
     9001 cctgccggcg ttgacgtaaa aatcaacggt caggttatta cgatcaaagg taaaaacggc
     9061 gagctgactc gtactctcaa cgatgctgtt gaagttaaac atgcagataa taccctgacc
     9121 ttcggtccgc gtgatggtta cgcagacggt tgggcacagg ctggtaccgc gcgtgccctg
     9181 ctgaactcaa tggttatcgg tgttaccgaa ggcttcacta agaagctgca gctggttggt
     9241 gtaggttacc gtgcagcggt taaaggcaat gtgattaacc tgtctctggg tttctctcat
     9301 cctgttgacc atcagctgcc tgcgggtatc actgctgaat gtccgactca gactgaaatc
     9361 gtgctgaaag gcgctgataa gcaggtgatc ggccaggttg cagcggatct gcgcgcctac
     9421 cgtcgtcctg agccttacaa aggcaagggg gttcgttacg ccgacgaagt cgtgcgtacc
     9481 aaagaggcta agaagaagta aggtaacact atggataaga aatctgctcg tatccgtcgt
     9541 gcgacccgcg cacgccgcaa gctccaggag ctgggcgcaa ctcgcctggt ggtacatcgt
     9601 accccgcgtc acatttacgc acaggtaatt gcaccgaacg gttctgaagt tctggtagct
     9661 gcttctactg tagaaaaagc tatcgctgaa caactgaagt acaccggtaa caaagacgcg
     9721 gctgcagctg tgggtaaagc tgtcgctgaa cgcgctctgg aaaaaggcat caaagatgta
     9781 tcctttgacc gttccgggtt ccaatatcat ggtcgtgtcc aggcactggc agatgctgcc
     9841 cgtgaagctg gccttcagtt ctaaggtaga ggtgtaagat ggctcacatc gaaaaacaag
     9901 ctggcgaact gcaggaaaag ctgatcgcgg taaaccgcgt atctaaaacc gttaaaggtg
     9961 gtcgtatttt ctccttcaca gctctgactg tagttggcga tggtaacggt cgcgttggtt
    10021 ttggttacgg taaagcgcgt gaagttccag cagcgatcca gaaagcgatg gaaaaagccc
    10081 gtcgcaatat gattaacgtc gcgctgaata acggcactct acaacaccct gttaaaggtg
    10141 ttcacacggg ttctcgcgta ttcatgcagc cggcttccga aggtaccggt atcatcgccg
    10201 gtggtgcaat gcgcgccgtt ctggaagtcg ctggggttca taacgttctg gctaaagcat
    10261 atggttccac caacccgatc aacgtggttc gtgcaactat tgatggcctg gaaaatatga
    10321 attctccaga aatggtcgct gccaagcgtg gtaaatccgt tgaagaaatt ctggggaaat
    10381 aaaccatggc aaagactatt aaaattactc aaacccgcag tgcaatcggt cgtctgccga
    10441 aacacaaggc aacgctgctt ggcttgggtc tgcgtcgtat tggtcacacc gtagagcgcg
    10501 aggatactcc tgctattcgc ggtatgatca acgcggtttc cttcatggtt aaagttgagg
    10561 agtaagagat gcgtttaaat actctgtctc cggccgaagg ctccaaaaag gcgggtaaac
    10621 gcctgggtcg tggtatcggt tctggcctcg gtaaaaccgg tggtcgtggt cacaaaggtc
    10681 agaagtctcg ttctggcggt ggcgtacgtc gcggtttcga gggtggtcag atgcctctgt
    10741 accgtcgtct gccgaaattc ggcttcactt ctcgtaaagc agcgattaca gccgaagttc
    10801 gtctatctga cctggctaaa gtagaaggcg gtgttgtaga cctgaacacg ctgaaagcgg
    10861 ctaacattat cggtatccag atcgagttcg cgaaagtgat cctggctggc gaagtaacga
    10921 ctccggtaac tgttcgtggc ctgcgtgtta ctaaaggcgc tcgtgctgct atcgaagctg
    10981 ctggcggtaa aatcgaggaa taagtagcag atggctaaac aaccgggatt agattttcaa
    11041 agtgccaaag gtggcttagg cgagctgaaa cgcagactgc tgtttgttat cggtgcgctg
    11101 attgtgttcc gtattggctc ttttattccg atccctggta ttgatgccgc tgtacttgcc
    11161 aaactgcttg agcaacagcg aggcaccatc attgagatgt ttaacatgtt ctctggtggt
    11221 gctctcagcc gtgcttctat ctttgctctg gggatcatgc cgtatatttc ggcgtcgatc
    11281 attatccagc tgctgacggt ggttcaccca acgttggcag aaattaagaa agaaggggag
    11341 tctggtcgtc gtaagatcag ccagtacacc cgttacggta ctctggtgct ggcaatattc
    11401 cagtcgatcg gtattgctac cggtctgccg aatatgcctg gtatgcaagg cctggtgatt
    11461 aacccgggct ttgcattcta cttcaccgct gttgtaagtc tggtcacagg aacgatgttc
    11521 ctgatgtggt tgggcgaaca gatcactgaa cgaggtatcg gcaacggtat ttcaatcatt
    11581 atcttcgccg gtattgtcgc gggactcccg ccagccattg cccatactat cgagcaagcg
    11641 cgtcaaggcg acctgcactt cctcgtgttg ctgttggttg cagtattagt atttgcagtg
    11701 acgttctttg ttgtgtttgt tgagcgtggt caacgccgca ttgtggtaaa ctacgcgaaa
    11761 cgtcagcaag gtcgtcgtgt ctatgctgca cagagcacac atttaccgct gaaagtgaat
    11821 atggcggggg taatcccggc aatcttcgct tccagtatta ttctgttccc ggcgaccatc
    11881 gcgtcatggt tcgggggcgg tactggttgg aactggctga caacaatttc gctgtatttg
    11941 cagcctgggc aaccgcttta tgtgttactc tatgcgtctg caatcatctt cttctgtttc
    12001 ttctacacgg cgttggtttt caacccgcgt gaaacagcag ataacctgaa gaagtccggt
    12061 gcatttgtac caggaattcg tccgggagag caaacggcga agtatatcga taaagtaatg
    12121 acccgcctga ccttggttgg tgcgctgtat attaccttta tctgcctgat cccggagttc
    12181 atgcgtgatg caatgaaagt accgttctac ttcggtggga cctcactgct tatcgttgtt
    12241 gtcgtgatta tggactttat ggctcaagtg caaactctga tgatgtccag tcagtatgag
    12301 tctgcattga agaaggcgaa cctgaaaggc tacggccgat aattggtcgc ccgagaagtt
    12361 acggagagta aaaatgaaag ttcgtgcttc cgtcaagaaa ttatgccgta actgcaaaat
    12421 cgttaagcgt gatggtgtca tccgtgtgat ttgcagtgcc gagccgaagc ataaacagcg
    12481 ccaaggctga ttttttcgca tatttttctt gcaaagttgg gttgagctgg ctagattagc
    12541 cagccaatct tttgtatgtc tgtacgtttc catttgagta tcctgaaaac gggcttttca
    12601 gcatggaacg tacatattaa atagtaggag tgcatagtgg cccgtatagc aggcattaac
    12661 attcctgatc ataagcatgc cgtaatcgca ttaacttcga tttatggcgt cggcaagacc
    12721 cgttctaaag ccatcctggc tgcagcgggt atcgctgaag atgttaagat cagtgagctg
    12781 tctgaaggac aaatcgacac gctgcgtgac gaagttgcca aatttgtcgt tgaaggtgat
    12841 ctgcgccgtg aaatcagcat gagcatcaag cgcctgatgg atcttggttg ctatcgcggt
    12901 ttgcgtcatc gtcgtggtct cccggttcgc ggtcagcgta ccaagaccaa cgcacgtacc
    12961 cgtaagggtc cgcgcaaacc gatcaagaaa taatcggggt gattgaataa tggcaaaggc
    13021 accaattcgt gcacgtaaac gtgtaagaaa acaagtctct gacggcgtgg ctcatatcca
    13081 tgcttctttc aacaacacca tcgtgactat cactgatcgt cagggtaacg cgttgggttg
    13141 ggcaacagcc ggtggttccg gtttccgtgg ttctcgcaaa tccactccgt ttgcagctca
    13201 ggttgcagca gagcgttgcg ctgacgccgt gaaagaatac ggcatcaaga atctggaagt
    13261 tatggttaaa ggtccgggtc caggccgcga atctactatt cgtgctctga acgccgcagg
    13321 tttccgcatc actaacatta ctgatgtgac tccgatccct cataacggtt gtcgtccgcc
    13381 gaaaaaacgt cgcgtataac gcctcgtttt ccaggtttgt tggagaaaga aaatggcaag
    13441 atatttgggt cctaagctca agctgagccg tcgtgagggc accgacttat tccttaagtc
    13501 tggcgttcgc gcgatcgata ccaagtgtaa aattgaacaa gctcctggcc agcacggtgc
    13561 gcgtaaaccg cgtctgtctg actatggtgt gcagttgcgt gaaaagcaaa aagttcgccg
    13621 tatctatggt gtgctggagc gtcagttccg taactactac aaagaagcag cacgtctgaa
    13681 aggcaacacc ggtgaaaacc tgttggctct gctggaaggt cgtctggaca acgttgtata
    13741 ccgtatgggc ttcggtgcca ctcgtgcaga agcacgtcag ctggttagcc ataaagcaat
    13801 tatggtaaac ggtcgtgttg ttaacatcgc ttcttatcag gttagtccga atgacgttgt
    13861 aagcattcgt gagaaagcga agaagcagtc tcgcgtgaaa gccgctctgg agctggctga
    13921 gcagcgtgaa aagccaactt ggctggaagt tgatgctggc aagatggaag gtacgtttaa
    13981 gcgtaagccg gagcgttctg atctgtctgc ggacattaac gaacacctga tcgtcgagct
    14041 ttactccaag taaagcttag taccaaagag aggacacaat gcagggttct gtgacagagt
    14101 ttctaaaacc gcgcctggtt gatatcgagc aagtgagttc gacgcacgcc aaggtgaccc
    14161 ttgagccttt agagcgtggc tttggccata ctctgggtaa cgcactgcgc cgtattctgc
    14221 tctcatcgat gccgggttgc gcggtgaccg aggttgagat tgatggtgta ctacatgagt
    14281 acagcaccaa agaaggcgtt caggaagata tcctggaaat cctgctcaac ctgaaagggc
    14341 tggcggtgag agttcagggc aaagatgaag ttattcttac cttgaataaa tctggcattg
    14401 gccctgtgac tgcagccgat atcacccacg acggtgatgt tgaaatcgtc aagccgcagc
    14461 acgtgatctg ccacctgacc gatgagaacg cgtctattag catgcgtatc aaagttcagc
    14521 gcggtcgtgg ttatgtgccg gcttctaccc gaattcattc ggaagaagat gagcgcccaa
    14581 tcggccgtct gctggtcgac gcatgctaca gccctgtgga gcgtattgcc tacaatgttg
    14641 aagcagcgcg tgtagaacag cgtaccgacc tggacaagct ggtcatcgaa atggaaacca
    14701 acggcacaat cgatcctgaa gaggcgattc gtcgtgcggc aaccattctg gctgaacaac
    14761 tggaagcttt cgttgactta cgtgatgtac gtcagcctga agtgaaagaa gagaaaccag
    14821 agttcgatcc gatcctgctg cgccctgttg acgatctgga attgactgtc cgctctgcta
    14881 actgccttaa agcagaagct atccactata tcggtgatct ggtacagcgt accgaggttg
    14941 agctccttaa aacgcctaac cttggtaaaa aatctcttac tgagattaaa gacgtgctgg
    15001 cttcccgtgg actgtctctg ggcatgcgct tggaaaactg gccaccggca agcatcgctg
    15061 acgagtaacc ggatcacagg ttaaggtttt actgagaagg ataaggtcat gcgccatcgt
    15121 aagagtggtc gtcaactgaa ccgcaacagc agccatcgcc aggctatgtt ccgcaatatg
    15181 gcaggttcac tggttcgtca tgaaatcatc aagacgactc tgcctaaagc gaaagagctg
    15241 cgccgcgtag ttgagccgct gattactctt gccaagactg atagcgttgc taatcgtcgt
    15301 ctggcattcg cccgtactcg tgataacgag atcgtggcaa aactgtttaa cgaactgggc
    15361 ccgcgtttcg cgagccgtgc cggtggttac actcgtattc tgaagtgtgg cttccgtgca
    15421 ggcgacaacg cgccgatggc ttacatcgag ctggttgatc gttcagagaa agcagaagc
//
LOCUS       DAJTNP010000088        15345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTNP010000088 DAJTNP010000000
VERSION     DAJTNP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO160_003075"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO160_003075"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5525105.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO160_003076"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO160_003076"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5525106.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO160_003077"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO160_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5525107.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO160_003078"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO160_003078"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5525108.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO160_003079"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO160_003079"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5525109.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO160_003080"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO160_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5525110.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO160_003081"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO160_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5525111.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO160_003082"
     CDS             complement(8065..9207)
                     /locus_tag="OO160_003082"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5525112.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO160_003083"
     CDS             complement(9216..10229)
                     /locus_tag="OO160_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5525113.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO160_003084"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO160_003084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5525114.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO160_003085"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO160_003085"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5525115.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO160_003086"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO160_003086"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5525116.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO160_003087"
     CDS             complement(12827..13951)
                     /locus_tag="OO160_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5525117.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO160_003088"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO160_003088"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5525118.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4382 a         3446 c         3144 g         4373 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccgata
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgg cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcaac aagaattggc gggtaaattg ctatttaagt caaaa
//
LOCUS       DAJTNP010000089        14978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTNP010000089 DAJTNP010000000
VERSION     DAJTNP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(520..1461)
                     /gene="fabY"
                     /locus_tag="OO160_003089"
                     /gene_synonym="yiiD"
     CDS             complement(520..1461)
                     /gene="fabY"
                     /locus_tag="OO160_003089"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5525119.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="OO160_003090"
     CDS             complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="OO160_003090"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5525120.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGVGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1940..2812)
                     /locus_tag="OO160_003091"
     CDS             complement(1940..2812)
                     /locus_tag="OO160_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5525121.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="OO160_003092"
     CDS             complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="OO160_003092"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5525122.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3504..4289)
                     /locus_tag="OO160_003093"
     CDS             complement(3504..4289)
                     /locus_tag="OO160_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5525123.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="OO160_003094"
     CDS             complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="OO160_003094"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5525124.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5387..6283
                     /gene="yihU"
                     /locus_tag="OO160_003095"
     CDS             5387..6283
                     /gene="yihU"
                     /locus_tag="OO160_003095"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5525125.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6307..7185
                     /gene="yihT"
                     /locus_tag="OO160_003096"
     CDS             6307..7185
                     /gene="yihT"
                     /locus_tag="OO160_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5525126.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7202..8443
                     /gene="yihS"
                     /locus_tag="OO160_003097"
     CDS             7202..8443
                     /gene="yihS"
                     /locus_tag="OO160_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5525127.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8581..9483
                     /locus_tag="OO160_003098"
     CDS             8581..9483
                     /locus_tag="OO160_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5525128.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9682..11718
                     /gene="yihQ"
                     /locus_tag="OO160_003099"
     CDS             9682..11718
                     /gene="yihQ"
                     /locus_tag="OO160_003099"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5525129.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11764..13138
                     /locus_tag="OO160_003100"
                     /pseudo
     CDS             11764..13138
                     /locus_tag="OO160_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13181..14584
                     /locus_tag="OO160_003101"
     CDS             13181..14584
                     /locus_tag="OO160_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5525130.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14652..>14978
                     /gene="ompL"
                     /locus_tag="OO160_003102"
     CDS             14652..>14978
                     /gene="ompL"
                     /locus_tag="OO160_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5525131.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3675 a         3856 c         3734 g         3713 t
ORIGIN      
        1 tgatttaata gcgtttgctt cggtgattta ttactcatta gttgtttttc taaattaaca
       61 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      121 cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt aaatgaatga
      181 tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta tagattttaa
      241 aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa atcgctcaaa
      301 cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag ctctacaaat
      361 aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca acgaagaata
      421 aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat gtaaatgacc
      481 ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt cgttcccgcc
      541 ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc
      601 acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg
      661 cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat ggggtttacc
      721 gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg
      781 gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca gcgagaataa
      841 actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca tggtggtgat
      901 aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa gcgggatatg
      961 ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa tatcatccag
     1021 agtggcgacg ggcttaatca tcaaaaaatg gcgaatcggc gtggtggttg gcgtggtgat
     1081 ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt cacgggcgct
     1141 acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca tcgccatcag
     1201 cgttcctaac cctttgtcct gcacgtccgg gtgaacgacc ataaagcgaa tggacgcttc
     1261 attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct cgtcgacgac
     1321 catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg gttgatgcag
     1381 gggcttacgc aacatttccc agcgaaagtg atagtaacgc tctaactctt cttctgtttg
     1441 tggaacctga aggtgataca tagcgatact ctctcttgtt tcccgtgaca accctggaag
     1501 ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga tacctgcata
     1561 tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg ttcgacgaaa
     1621 tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact tgggcgcatc
     1681 ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact gccgcccgcc
     1741 tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta gccgagcaca
     1801 cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc caataacacc
     1861 aaaagtccca cgccaatttc gcccgtcact tctccctcca cggtgacgct ggcacgggtt
     1921 acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct gcttgtttta
     1981 gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg atacaccatg
     2041 tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa atgagctgat
     2101 atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg aacaggagtg
     2161 cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc
     2221 tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc agcacgttgt
     2281 cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa ctgatcgcca
     2341 gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg gcgaacgagt
     2401 aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta tcgatggagt
     2461 acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc atcttgttgg
     2521 aattagcaac aaattgttcg atataccgct gaataacatc gccagtagca ggcagaaagt
     2581 tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga aaagcggcga
     2641 aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca taggcaaggt
     2701 tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt tttagccagg
     2761 cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc ataacacctt
     2821 cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa tgcctagctg
     2881 attagctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa
     2941 accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca tccccagatc
     3001 ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt ccggccagaa
     3061 ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct gctcacgcag
     3121 tttatgcatg atggcgatca cttccgggcg cagcgcaaca aacaccgcct gccagccgtg
     3181 agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct ctgcgaacgc
     3241 ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat gaaaactctt
     3301 cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca cacggttaaa
     3361 gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg cctcctttcg
     3421 cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc aaggtatgaa
     3481 gccagattaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat ttcaatttgg
     3541 ctgtcttgta agcgtgattt tagcgcagcg ggcggcagct cgtcggtaaa gagcgcagtg
     3601 acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt gtgatcggcg
     3661 accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc ttcgtttaca
     3721 tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac cagataatca
     3781 gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct attatgagag
     3841 cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag aatatgcgcc
     3901 acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc ccgggcaaca
     3961 tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat atagtctgcc
     4021 accgcttcgg caatcgcttt ttttttctcg gtttgcgaaa cctcacgctg ctcgaacgcc
     4081 gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc
     4141 tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag cagatttgcc
     4201 agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc
     4261 gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat caccctggca
     4321 ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat cccggctcgt
     4381 ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac tgactccgct
     4441 aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc tcccgcacct
     4501 gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc attttccagc
     4561 cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag cgtttgtgcc
     4621 tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc cggttctgaa
     4681 aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt aatgtccccg
     4741 tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt agcgccgtcg
     4801 tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc ctccaaccac
     4861 tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc gcctttggta
     4921 tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt gtaacgggtg
     4981 ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgcgtcgtc atcacctacg
     5041 cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc cgtcgccgct
     5101 ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc cgtcggtaac
     5161 ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac acgaatcatg
     5221 gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt ttaaaaagtg
     5281 acggctgtca attttttgac gataaattac aaattcgatc aaaaacagac aagcaaagca
     5341 tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg cagcaatcgc
     5401 gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc agcaagggca
     5461 tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag acaaaggtgc
     5521 gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca ttaccatgtt
     5581 gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt gcgaaggctt
     5641 atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa ccgataaatt
     5701 gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag gccgtacttc
     5761 tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg aacaagttga
     5821 acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaacgctg gcggtccggg
     5881 catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca atgcgctttc
     5941 ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt
     6001 gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa acaaagtcct
     6061 cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc ttggcatcgc
     6121 ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt
     6181 ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca
     6241 ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg ataaaggaat
     6301 aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg ttttgccatg
     6361 ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg ggcacccgct
     6421 ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc cctctcgcct
     6481 tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt tgagcaaaac
     6541 gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc tggcaacggt
     6601 attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat caaacaagac
     6661 ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc gcagcaacgt
     6721 ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt aagcatcatt
     6781 gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca agcgatcatc
     6841 gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga aatgccgctt
     6901 tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa tgaccatatc
     6961 aatatgccat gggtgatcct ctcttccagt gtcgacgaaa aactgttccc gcgtgccgta
     7021 cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt ctgggcatcg
     7081 gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg tttgcgcacc gaaattacaa
     7141 caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga ggataaaagg
     7201 aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa ccgaccgcat
     7261 ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag gcaataaagg
     7321 gcaaatcaaa gaagagatgg gcacccatct gtggatcaca gctcgtatgt tgcacgttta
     7381 ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg ttggttgatc acggtattaa
     7441 agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct gcgtgaatga
     7501 cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc tgctgggtgc
     7561 cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt acaccattga
     7621 gattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggagt cctgggacga
     7681 agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg cggtggaagc
     7741 tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg cgattcgcgt
     7801 ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta acgaacactt
     7861 cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc atcgcttccg
     7921 cgcgttcgga ggtacaccag gccactggat cgaatggggc cgtttaatgc tgcacatcca
     7981 tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag atgccaaagg
     8041 tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggagcggacg ggattgttta
     8101 taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc ctatcgtcga
     8161 agcaatgggt acggcctacg cgctctacac cgtcactggc gatcgccagt atgaaacctg
     8221 gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa atggttcctg
     8281 gtggcaggag ctggatgcgg acaataaggt catcaccaaa gtctgggacg gcaaacagga
     8341 tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc ccggcatggc
     8401 tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct gaaaataggg
     8461 tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccggtgca
     8521 gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa ggtgccttgt
     8581 atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc gggggattat
     8641 cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca gggttgtcat
     8701 ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa agtgcttatt
     8761 ccgtggccga accggatcgc caacggctgt taccgttatc aggggcagga atatcagtta
     8821 cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg gcgagactgg
     8881 caaattagtg aattaaccac caccagcgtt accctgacag cttttctgcc gcccagttac
     8941 ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca cacgggtctt
     9001 tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg cgtgggtatt
     9061 catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca gcttccggca
     9121 aaccaggttt atgccgtcga tgaacacgta aatccgacaa cgcttcacca cgttgatgag
     9181 ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga ccacaccttt
     9241 aaaaccgcta atgatttgtg ggaaatgacc attacccatc cacagcaggc gctttcggta
     9301 agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt acagcgtcag
     9361 ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg tatcgactta
     9421 ttattactgg aaccaggaaa accgcaccgc ctgtttttta atatttacgg acagagaaaa
     9481 taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct aaaggcaaca
     9541 ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt ttttatatat
     9601 cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac aatctcgttc
     9661 tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga ttttcaattt
     9721 catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt aacccataac
     9781 aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat gttccgcggt
     9841 aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc catcgtcagc
     9901 cagtcaccgg atggttggtt aattcatttc agccgtggtt ctgacattag cgccacgctg
     9961 aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga caaccttaac
    10021 cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta cggctgcggc
    10081 gaacagtttt cctacttcga tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa
    10141 ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa agaaaatgcg
    10201 ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac gcagaagtat
    10261 tactgccatg ttgataacag ttgctacatg aacttcgact ttagcgcccc ggaataccat
    10321 gaactggcgc tgtgggaaga caaagctacg ctacgttttg aatgtgctga cacatacatc
    10381 tccctgctgg aaaaattaac tgccctgctg ggacgccagc cagaactgcc cgactggatt
    10441 tatgacgggg taacgctcgg cattcagggc gggacggaag tgtgccagaa gaaactggac
    10501 accatgcgta acgcgggcgt gaaggttaac ggcatctggg cgcaggactg gtctgggatc
    10561 cgtataacct cttttggcaa gcgcgtgatg tggaactgga agtggaacag cgaaaactac
    10621 ccgcaactgg attcacgcat taagcagtgg tatcaggagg gcgtgcagtt cctggcctat
    10681 atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca acacggctat
    10741 ctagcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt ttacggcggc
    10801 gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat caaaaagaac
    10861 atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct gcccaccgac
    10921 acgtacctgc ataacggcgt cagtgccgaa attatgcata acgcctggcc tgcgctgtgg
    10981 gcgaagtgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat cctcttcttt
    11041 atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc gggcgaccag
    11101 aacgtggact ggagtctcga cgatggcctg gcgtccgtag tcccggcggc gctgtcgctg
    11161 gcaatgaccg gacatggtct gcatcacagc gacattggcg gttacaccac cctgtttgag
    11221 atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg
    11281 atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg cgatgcggaa
    11341 actatcgccc attttgcccg tatgactacc gttttcacca ccctgaaacc ttatctgaaa
    11401 gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca tgcgcccgct gttcctgcat
    11461 tacgaagacg atgcacatac ctattccctg aaatatcagt acctgttagg ccgcgacatt
    11521 ctggtcgctc cggtacacga agaaggtcgc agcgactgga cgctctatct gccggaggat
    11581 aactgggtac atgcctggac gggtgaagcg ttccggggcg gggaagttac cgttaatgcg
    11641 cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc actgttcgcg
    11701 tcgttaaaca gcatctaatc acatccgccc gtggggaaac tcatgggcaa agggagaaca
    11761 tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt taaagagaaa
    11821 ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg tacgctttat
    11881 cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg cattatcttt
    11941 ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat gttggattcc
    12001 cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc gtcattcccg
    12061 gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg
    12121 gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat gaactgctcc
    12181 tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg aacgcgcctc actggcggca
    12241 tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt cgtgccagtt
    12301 atgaatctta tcgaaggtaa tcagaaactt ggctatatct tcgccgccac gctgttttca
    12361 ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg ttatgtcgaa
    12421 acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg cgcaattgcc
    12481 ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg ggcgtttaac
    12541 gtcaagctcg ccattcaggt ctattacacc cagtacgtac ttaacgatcc catcctgttg
    12601 tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct gatgcctggc
    12661 gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg
    12721 ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc gttctcctgc
    12781 ctggcgttct tcggctcagc gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg
    12841 cgagtggcgc accggcgtgc gttcggaagg cacggtctac accggcttca ccttctttcg
    12901 caaagtgtct caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaattggcta
    12961 tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg ttacgccagt tgatcttcat
    13021 ctacccaagc gcactggcgg tagtcaccat tgtggcgatg ggttgcttct acagcctgaa
    13081 cgagaagatg tatgtccgca ttgtggaaga gatagaagcc cgtaaacgca cggcgtaata
    13141 ataataaacg acgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact
    13201 gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg tcggttcgaa
    13261 tttaatgctc tgcatcggta ctctgtatct cctcaaactt tataccgatg aattagggat
    13321 gcctgcttac tatggcggga ttatctttct ggtcgcgaag ttttttaccg cgtttaccga
    13381 tatgctcacc ggatttttac tcgactcgcg gaaaaatatt gggcctaaag gcaaatttcg
    13441 tccctttatt ttatatgcag cagttccggc ggcgttaatt gcaacgctcc agtttatcgc
    13501 caccaccttt tgtttgccgg ttaaaacgac gattgccacc acgctgttta tgatgtttgg
    13561 actttcatac agtctgatga actgctcgta tggtgcgatg atcccggcaa ttaccaaaaa
    13621 cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt
    13681 gatttgtacc gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta
    13741 tgcctgtgcg gcacttatgt tctccattgg cggctttatt tttatgatgc tgtgctacag
    13801 aggcgtcaaa gagcattatg tggacacagc gccaaccgga cataaagcca gtattctcaa
    13861 atctttttgc gcgatatttc gtaatccacc attgctggtt ttatgcattg ctaacctgtg
    13921 tactctggcg gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct
    13981 gaatgatatt aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg
    14041 cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg
    14101 catggtgcta tgggcggtgg gtgatatact gaattatttc tggggaagta actctttcac
    14161 tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc tttgtaaaca gcctgaactg
    14221 ggcactggta ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg
    14281 ttctgtttat accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt
    14341 gccaggcatt atgctgacgc aaattggtta tgttcccaac atcgcgcaaa gcgatgcgac
    14401 attacagggt ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc
    14461 attgacgatg ggattctttt acacactcaa tgaaaaacgg tttgcattga ttattgagga
    14521 aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa aaaacggctt ccgtaacgtt
    14581 ataaatgtca atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa
    14641 atggacttat tatgaaaaag attaatgcaa taattttatt atcttctctg acctctgcct
    14701 cggtatttgc tggcgcatac gtcgaaaaca gggaggcata taatcttgcc tccgatcagg
    14761 gcgaggttat gctacgtgtg ggttataact tcgatatggg cgcgggtatt atgttaacca
    14821 atacctacac ctttcagcga gaagatgaac taaaacatgg ctataatgaa attgaaggtt
    14881 ggtatccttt attcaaacca accgataaat taaccatcca gcccggtggc ttaattaatg
    14941 ataagagtat cagttcgggt ggtgcagtgt atctggac
//
LOCUS       DAJTNP010000090        14471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTNP010000090 DAJTNP010000000
VERSION     DAJTNP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(105..371)
                     /locus_tag="OO160_003103"
     CDS             complement(105..371)
                     /locus_tag="OO160_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5525132.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            645..4169
                     /gene="nifJ"
                     /locus_tag="OO160_003104"
     CDS             645..4169
                     /gene="nifJ"
                     /locus_tag="OO160_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5525133.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4536..5665
                     /gene="ompN"
                     /locus_tag="OO160_003105"
                     /pseudo
     CDS             4536..5665
                     /gene="ompN"
                     /locus_tag="OO160_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5806..6240
                     /gene="uspF"
                     /locus_tag="OO160_003106"
     CDS             5806..6240
                     /gene="uspF"
                     /locus_tag="OO160_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5525134.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6416..7351
                     /gene="ttcA"
                     /locus_tag="OO160_003107"
     CDS             6416..7351
                     /gene="ttcA"
                     /locus_tag="OO160_003107"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5525135.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7480..8847)
                     /gene="dbpA"
                     /locus_tag="OO160_003108"
     CDS             complement(7480..8847)
                     /gene="dbpA"
                     /locus_tag="OO160_003108"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5525136.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9325..10308)
                     /gene="zntB"
                     /locus_tag="OO160_003109"
     CDS             complement(9325..10308)
                     /gene="zntB"
                     /locus_tag="OO160_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5525137.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10563..11795
                     /gene="dgcM"
                     /locus_tag="OO160_003110"
                     /pseudo
     CDS             10563..11795
                     /gene="dgcM"
                     /locus_tag="OO160_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11816..12379)
                     /gene="smrA"
                     /locus_tag="OO160_003111"
                     /pseudo
     CDS             complement(11816..12379)
                     /gene="smrA"
                     /locus_tag="OO160_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12633..14087)
                     /locus_tag="OO160_003112"
     CDS             complement(12633..14087)
                     /locus_tag="OO160_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5525138.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14084..>14471)
                     /locus_tag="OO160_003113"
     CDS             complement(14084..>14471)
                     /locus_tag="OO160_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5525139.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3857 a         3648 c         3418 g         3548 t
ORIGIN      
        1 agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg
       61 accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag
      121 ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca
      181 atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt
      241 gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc cgcagtcgcc
      301 tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc tacccaaaac
      361 gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag
      421 gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt
      481 atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg tgggggacac
      541 aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc
      601 agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg
      661 gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc atctacccta
      721 ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga
      781 acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta
      841 ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt
      901 tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca tttgtcctgc
      961 atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat cattccgacg
     1021 ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc caggaagcgc
     1081 aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca tttattcatt
     1141 tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca
     1201 ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc
     1261 cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg
     1321 aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg atgaatgatt
     1381 tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat ccgcaagcgg
     1441 aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat
     1501 tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt cccttctccg
     1561 ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa
     1621 ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag
     1681 aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg cgctatggtc
     1741 tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg
     1801 ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat ctgtcactgc
     1861 cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt tatggccttg
     1921 gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt aattccacgc
     1981 cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc ctgacggttt
     2041 ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt
     2101 ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac
     2161 ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc
     2221 cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg
     2281 cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt
     2341 ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga
     2401 ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg caggctctgg
     2461 cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca
     2521 atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcggcga
     2581 tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc
     2641 cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag
     2701 aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca gctattcgcg
     2761 caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctagatg
     2821 tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca
     2881 ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta
     2941 aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc aattacgatt
     3001 tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat
     3061 cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt ggcgagacgc
     3121 cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct aacgccaccg
     3181 gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg
     3241 gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt
     3301 tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg
     3361 ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc
     3421 gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaacttc
     3481 tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc gacggctggg
     3541 cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca
     3601 ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac
     3661 cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg
     3721 gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc
     3781 tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca tcgctgatca
     3841 ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac gaccagatgc
     3901 gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt cgtgccgatg
     3961 aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt
     4021 tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt
     4081 tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca caaatggccg
     4141 gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa
     4201 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
     4261 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc gcgcagaata
     4321 acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg atacattcac
     4381 cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag gcgtaacaaa
     4441 tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc gaaatgggga
     4501 taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta ctggcacttt
     4561 taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat aataaagacg
     4621 gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct gataattcag
     4681 cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc caaattaacg
     4741 atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac actgaatctt
     4801 caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca gattacggtt
     4861 ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg accgatatgc
     4921 tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact ggtcgagcca
     4981 atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt ctgaacttcg
     5041 cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc accaataacg
     5101 aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca tatgatttag
     5161 gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat gaccaggtta
     5221 accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta aaatacgatg
     5281 ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc ccgtttggcg
     5341 acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt accagtttga
     5401 tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc ttcacgctgc
     5461 tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt
     5521 tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata aaatcaacct
     5581 gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg atattgtcgc
     5641 tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg
     5701 cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg
     5761 tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt
     5821 cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag ccacgttgag
     5881 gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc ttcactgccc
     5941 tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa
     6001 gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga
     6061 gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagaag
     6121 atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc
     6181 ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga
     6241 cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt
     6301 tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt atgctgagcg
     6361 ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc
     6421 gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac aaaaacgtct
     6481 gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag gcgatcgcat
     6541 catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc tgcgcaattt
     6601 gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc aaaagcaacc
     6661 gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg agtacaagat
     6721 tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca aaaccacttg
     6781 ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg aactggggac
     6841 gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt tcttaaatat
     6901 gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg atggcaaaca
     6961 tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat ttgccgatgc
     7021 aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca
     7081 ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta tcgagacgat
     7141 gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc tgttcgattt
     7201 caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt ttgatcgcga
     7261 agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc agttggatga
     7321 gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc gcaagcgtat
     7381 cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg caatcccgaa
     7441 taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata accgcacccg
     7501 gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat gagcaacagc
     7561 ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa tatctgcgcc
     7621 atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca ttttggcttt
     7681 tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa tggaactatt
     7741 agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga tattggcccg
     7801 ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg cacgagctgt
     7861 acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt tcaccaccag
     7921 ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca gtacacgggc
     7981 gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc cgtgtaacga
     8041 caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat cttttttggt
     8101 attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt gcaacagagg
     8161 aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca aagcatctgt
     8221 tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg cggcgatggc
     8281 ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa aacggatgac
     8341 atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat ccatcaccaa
     8401 agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca aacgacccgg
     8461 cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac cgaacggttg
     8521 accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc
     8581 acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct gggtttgaaa
     8641 tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc
     8701 ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg cggcggcctg
     8761 caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg cgggaggcaa
     8821 aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac gcaaagttag
     8881 caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg ttcttcatcg
     8941 ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg aacaggatcg
     9001 ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat atttccctcc
     9061 ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa aaaaagccga
     9121 ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt
     9181 aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca agggagatat
     9241 tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt ttttgtatac
     9301 agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc ataaagcaac
     9361 accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt gccacccgcc
     9421 gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac tgggtaaaaa
     9481 gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct gcatcacctg
     9541 agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt cgtcaaggcc
     9601 gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg
     9661 caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat agcgacgcat
     9721 cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt gctgatcaag
     9781 gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt cactggaatg
     9841 atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg gacccgtgcc
     9901 ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc gggtcgaaac
     9961 aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc gttcatcggt
    10021 gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc tgactcgggg
    10081 ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc
    10141 cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg gatgcgcttc
    10201 atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca gcatccaggc
    10261 aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg tgcctccatc
    10321 atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc tcatcacccc
    10381 attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt
    10441 tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa ttatctgatc
    10501 atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat aacgggatct
    10561 caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc tggattgttt
    10621 cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg atgcaagacc
    10681 tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa gaactgccga
    10741 aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact gttcagcgta
    10801 aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca gaaacagaac
    10861 cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa gccagtcgct
    10921 cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg actaactctg
    10981 caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct aacctcgccg
    11041 cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc taggaaataa
    11101 atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta cccggcggtc
    11161 ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat gtgcagacct
    11221 atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg catgatatta
    11281 ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac gcgatgaccg
    11341 gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg cagcatctcg
    11401 ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa cacattaacg
    11461 atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc cccctcgaaa
    11521 gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt gtcttattgc
    11581 taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc gtaagcgtgg
    11641 caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg gcgcatcacg
    11701 aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg tatcgggcga
    11761 aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct cgagatcaac
    11821 gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt ttacgtagtg
    11881 cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat gcctgaacat
    11941 catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca tgtgatttat
    12001 cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc agggcttgtt
    12061 gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa agattcaggc
    12121 tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc tacaaccctt
    12181 cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg gtagtgagga
    12241 aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc gttggatgcc
    12301 agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca agaaacagcg
    12361 atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga ttcagacggg
    12421 ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac tctacttcat
    12481 acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag tatcgaacag
    12541 tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca tagcaggaac
    12601 caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga aaatgccata
    12661 gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag tatttccgat
    12721 gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa gtcattcacc
    12781 gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt agttcgatta
    12841 ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg tgttaaatta
    12901 aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt taatgttacg
    12961 atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa cagtaattcc
    13021 tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac aaatccgccc
    13081 agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa gtaaaaggga
    13141 acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc ttcggcattt
    13201 aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa tatcccaacc
    13261 cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa agcatgaaga
    13321 tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa actcaccaat
    13381 gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg cgctccttct
    13441 tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact cggataatca
    13501 gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg aatactcaaa
    13561 actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc gaggttatca
    13621 gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag cgcactgact
    13681 accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat cgtgaaaact
    13741 aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat catgatctga
    13801 atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc ggatagagta
    13861 aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat aacaataatc
    13921 tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc ggacagacca
    13981 ccacctattt catttaggga tgacgataca atttgaatat caagctgagc aataaataat
    14041 cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc tcatcaacac
    14101 tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg tcgtcaaatg
    14161 caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca ttttctatcg
    14221 gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga ccgtgaaaca
    14281 cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc attcccgcta
    14341 tttggttttc tttataattt gcatcaaccc acaactcact gactggtact agtgaaacta
    14401 acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg acaatgccat
    14461 caatcggcgc a
//
LOCUS       DAJTNP010000091        14416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTNP010000091 DAJTNP010000000
VERSION     DAJTNP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..132)
                     /locus_tag="OO160_003114"
     CDS             complement(<1..132)
                     /locus_tag="OO160_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5525140.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO160_003115"
     CDS             315..458
                     /locus_tag="OO160_003115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525141.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO160_003116"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO160_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5525142.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO160_003117"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO160_003117"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5525143.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO160_003118"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO160_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5525144.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO160_003119"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO160_003119"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5525145.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO160_003120"
     CDS             complement(7051..7659)
                     /locus_tag="OO160_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5525146.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO160_003121"
     CDS             complement(7730..8815)
                     /locus_tag="OO160_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5525147.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO160_003122"
     CDS             complement(8818..9180)
                     /locus_tag="OO160_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5525148.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO160_003123"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO160_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5525149.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO160_003124"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO160_003124"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5525150.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO160_003125"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO160_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5525151.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO160_003126"
     CDS             complement(13472..13681)
                     /locus_tag="OO160_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525152.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="OO160_003127"
     CDS             13965..14327
                     /locus_tag="OO160_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5525153.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3522 a         3840 c         3624 g         3430 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaa
//
LOCUS       DAJTNP010000092        13968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTNP010000092 DAJTNP010000000
VERSION     DAJTNP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..3001
                     /locus_tag="OO160_003128"
                     /pseudo
     CDS             1..3001
                     /locus_tag="OO160_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3011..6657
                     /locus_tag="OO160_003129"
                     /pseudo
     CDS             3011..6657
                     /locus_tag="OO160_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6718..7125
                     /locus_tag="OO160_003130"
     CDS             6718..7125
                     /locus_tag="OO160_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525154.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7667..8755
                     /locus_tag="OO160_003131"
     CDS             7667..8755
                     /locus_tag="OO160_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5525155.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8766..11045
                     /locus_tag="OO160_003132"
     CDS             8766..11045
                     /locus_tag="OO160_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525156.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11038..12175
                     /locus_tag="OO160_003133"
                     /pseudo
     CDS             11038..12175
                     /locus_tag="OO160_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12172..>13968
                     /locus_tag="OO160_003134"
     CDS             12172..>13968
                     /locus_tag="OO160_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525157.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLM"
BASE COUNT         3455 a         3681 c         3570 g         3262 t
ORIGIN      
        1 gactacttgg agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg
       61 gaagagtctc tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca
      121 gaatggcggc aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa
      181 attgcccagc atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg
      241 gcgactgatg agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt
      301 gatgattact attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca
      361 gccctgcccc gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat
      421 atcaatcatc cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt
      481 cacgatcgaa tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg
      541 gaacttctag tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc
      601 tcgcaaccag cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg
      661 ctgaaatcat gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg
      721 ccagccatag tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg
      781 ctggatttag cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat
      841 caacttttgg cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt
      901 actaccaacc tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa
      961 gctcccgaag cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa
     1021 tttaaggtgt ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg
     1081 ccgctggagg ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac
     1141 cgataccgaa tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc
     1201 aattacacgc tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct
     1261 ggctcctgcc gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac
     1321 ctggctgttg aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa
     1381 agccggtgaa gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca
     1441 tcagccatta ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt
     1501 gaacgctctg cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc
     1561 gaccttttat cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact
     1621 gccagataac gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct
     1681 gtatccggga ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc
     1741 ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt
     1801 cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg
     1861 cgcctggcct ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc
     1921 tatcggtagt gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa
     1981 agcattacag gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac
     2041 ggtagcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt
     2101 gctgctggat tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt
     2161 tgtgcgtgat gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta
     2221 tgagtcacag gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac
     2281 cgtcgcggcc cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc
     2341 gccggaaaac ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg
     2401 tctgatttgg ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga
     2461 agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc
     2521 tatcggcatt cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct
     2581 ttttgccgac tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct
     2641 gacagaagcc gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag
     2701 cggccgggtc atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg
     2761 ctggatgatc aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc
     2821 ggtgggtata tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg
     2881 ggaaggcaaa gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc
     2941 cgtactcgat gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata
     3001 aggaaagagc atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa
     3061 atataaaaaa ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt
     3121 tatcactgaa agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa
     3181 aaaaaacatt gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca
     3241 ggccatcaac ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact
     3301 agcctttatc gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg
     3361 gctggaatat gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag
     3421 tgatcctctg gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc
     3481 agaaacctat aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag
     3541 ctgctggcag cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg
     3601 ccgccctttt attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag
     3661 ccttgaatgc ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga
     3721 ccccaaagcg gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga
     3781 agccagctac atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg
     3841 cgaacaagga ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg
     3901 cagtctgctg gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga
     3961 taaaaacaaa cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac
     4021 actcgccgca ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc
     4081 aatcatcgaa gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac
     4141 gctgttacaa acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt
     4201 aagtactcaa gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat
     4261 tgatagcacc gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag
     4321 aaagaaaaag acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac
     4381 gtacaggtcg ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag
     4441 cgccatgtct tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt
     4501 aggttttgac gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc
     4561 ctttgatgct cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc
     4621 gcatcaggcc aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga
     4681 tagcattgcg ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga
     4741 acagcgttgg gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt
     4801 atcgtgctgg caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt
     4861 aagtgtgctg ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa
     4921 agagatattt ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg
     4981 cgtctggcac ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc
     5041 ggaacatacg gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga
     5101 agctgcatta cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac
     5161 tgtagccaat cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca
     5221 gagtccaata gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca
     5281 aatgtggccc aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga
     5341 aattatcggc gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca
     5401 actgaaaacg ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc
     5461 gtggcaacaa gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt
     5521 tggtgacgaa agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg
     5581 caaatctgcc cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc
     5641 gctgatgcag ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc
     5701 ggcggaattt cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga
     5761 cagattagtc ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc
     5821 ccggcagttt accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa
     5881 cgttcgccag aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga
     5941 ggcactggcc cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct
     6001 cccgcgtctt gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc
     6061 tctgtttgtt aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat
     6121 ccggcaaatg aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc
     6181 tgcaatgcgc aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac
     6241 ccgttagaaa tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa
     6301 attatgccgc cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca
     6361 accagtaaca gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga
     6421 atgcgataca aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag
     6481 taccggctgg tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg
     6541 ctaatgagct tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa
     6601 cttgatgtgt ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac
     6661 aaagcgggca gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg
     6721 atcgtgcaaa aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg
     6781 ttttccttca gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga
     6841 ctggaacggt tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg
     6901 ctaataaatg atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga
     6961 ggcgaatcac ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac
     7021 caacaaatag aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat
     7081 gacgagtata gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg
     7141 cccgttactt caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa
     7201 tgagcacggt taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac
     7261 accagcacct tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg
     7321 ttgcactccg gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa
     7381 taggttcgat ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg
     7441 caacactgtc cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg
     7501 gtgttaggcc tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta
     7561 ctgtgatctg ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc
     7621 gcgtaaaata tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa
     7681 taatcatctg cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa
     7741 acaaaatgat aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc
     7801 ttttatcctt ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc
     7861 tgtcgaacgt atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc
     7921 tggcaccgca aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg
     7981 gttaaccatc cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta
     8041 cgccctgctt atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca
     8101 gggaatgcgc gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt
     8161 gcaagactgt ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg
     8221 tgaagccagt cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg
     8281 cgatcgcggc gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt
     8341 ctttccgatt atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt
     8401 gctggcccat agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg
     8461 caccttccgc gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc
     8521 gattatgtcc accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt
     8581 tttagcgaat cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt
     8641 caaagataac gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac
     8701 acataaagaa gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa
     8761 gatgcatgag cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc
     8821 tggtgaaatc gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg
     8881 attttaatga ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca
     8941 gttattgcca gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg
     9001 aattttcgcc ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact
     9061 tcatcgattt gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag
     9121 aggaaagcca gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg
     9181 atcacttgtt cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt
     9241 ttgcccaact gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta
     9301 tggcccgctg gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg
     9361 gtggctggca cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca
     9421 cgccagaatt gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg
     9481 aaaagcgcct tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa
     9541 actggtgctg gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca
     9601 cccgtttgcg ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc
     9661 atgcgatggc actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg
     9721 atgccatagc aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc
     9781 gcggcgttat tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg
     9841 gtgacggatt cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca
     9901 cttgcgaact ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta
     9961 accccaatgg actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag
    10021 ggattgtacg ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga
    10081 aattaacccg cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca
    10141 cactcctgga agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg
    10201 gcagcattac cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac
    10261 aattactgga gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc
    10321 aggcgctgga agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg
    10381 cgcagatatt acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat
    10441 ccaacggcag accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt
    10501 gtcatatcct gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg
    10561 cggtagcctt gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg
    10621 cgactcttgg tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga
    10681 cgatgctggc gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat
    10741 tagcccgtca tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa
    10801 atcaactaag tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct
    10861 ggctaccgcc acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg
    10921 cgcaacttcc cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac
    10981 atcatcaaca actcgaacag caggcactgg catcgctgca acactgggga gttttccatg
    11041 tctgaactga acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc
    11101 gaagcggcag aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg
    11161 ctggagtggc tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga
    11221 ttaggtggct caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg
    11281 caacaggtga ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg
    11341 gtgacaaatc tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt
    11401 ttgcacacca aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc
    11461 caggttgtgg aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt
    11521 gtccgcgatc gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt
    11581 actctgcgcg ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc
    11641 ccccgcttta acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt
    11701 gatcaaagcg gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta
    11761 tggcagttac ccggcattcg tacccatctg gtggcgtttg accacaagcg tcgttgatct
    11821 cacggcagac gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac
    11881 caatatcgcc agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt
    11941 cattatcctc gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt
    12001 gaaaaagtgt gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc
    12061 aacaccttgc tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc
    12121 cgccatgaca ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca
    12181 ctacgtccgg aattattaga actgacaccg caggcactga cggcgttaag caatgccggt
    12241 tttgttaagc gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag
    12301 aacagcgctt taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag
    12361 gcactgaaag aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg
    12421 ctggtgttaa gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa
    12481 tgggatccgg caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc
    12541 gcacaggcgc tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt
    12601 ccctctgccc gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc
    12661 gcccgttgtg attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc
    12721 tttgtccagg caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc
    12781 gaacacgtca catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa
    12841 tatgttcaac aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac
    12901 tacgaggccg ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg
    12961 agtgaatcgc tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt
    13021 gatgccttcc acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg
    13081 gcattaaaca gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa
    13141 gttcctcctg tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt
    13201 gatcatctgc ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt
    13261 ttacgcatct ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg
    13321 ccgcgaagtg aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct
    13381 ggcgcactgg cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag
    13441 ctgctgttag ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg
    13501 caaatgttga gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc
    13561 cagcgtcccc ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg
    13621 gtcgctgagt gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta
    13681 atcagcggcg aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg
    13741 ccttatgcca ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg
    13801 gtttctggct ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg
    13861 acaaagactc gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct
    13921 tctgccagcg ttctgcctgt gccgtctact gctcatcagt tgctgatg
//
LOCUS       DAJTNP010000093        13954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTNP010000093 DAJTNP010000000
VERSION     DAJTNP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO160_003135"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO160_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5525158.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO160_003136"
     CDS             2219..2767
                     /locus_tag="OO160_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5525159.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO160_003137"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO160_003137"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5525160.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO160_003138"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO160_003138"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5525161.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO160_003139"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO160_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5525162.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO160_003140"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO160_003140"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5525163.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO160_003141"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO160_003141"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5525164.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO160_003142"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO160_003142"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5525165.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO160_003143"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO160_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5525166.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13954)
                     /gene="ssuC"
                     /locus_tag="OO160_003144"
     CDS             complement(13351..>13954)
                     /gene="ssuC"
                     /locus_tag="OO160_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5525167.1"
                     /translation="ISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQMLRNV
                     PHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYGLSG
                     IPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQTDV
                     VVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3548 a         3560 c         3524 g         3322 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagtg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatc
//
LOCUS       DAJTNP010000094        13410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTNP010000094 DAJTNP010000000
VERSION     DAJTNP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..188
                     /locus_tag="OO160_003145"
     CDS             <1..188
                     /locus_tag="OO160_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5525168.1"
                     /translation="KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            complement(218..1201)
                     /gene="kdgT"
                     /locus_tag="OO160_003146"
     CDS             complement(218..1201)
                     /gene="kdgT"
                     /locus_tag="OO160_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5525169.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1461..2081)
                     /gene="sodA"
                     /locus_tag="OO160_003147"
     CDS             complement(1461..2081)
                     /gene="sodA"
                     /locus_tag="OO160_003147"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5525170.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2366..3400
                     /gene="rhaT"
                     /locus_tag="OO160_003148"
                     /pseudo
     CDS             2366..3400
                     /gene="rhaT"
                     /locus_tag="OO160_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3397..4263)
                     /gene="rhaR"
                     /locus_tag="OO160_003149"
     CDS             complement(3397..4263)
                     /gene="rhaR"
                     /locus_tag="OO160_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5525171.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4319..5155)
                     /gene="rhaS"
                     /locus_tag="OO160_003150"
     CDS             complement(4319..5155)
                     /gene="rhaS"
                     /locus_tag="OO160_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5525172.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5443..6912
                     /gene="rhaB"
                     /locus_tag="OO160_003151"
     CDS             5443..6912
                     /gene="rhaB"
                     /locus_tag="OO160_003151"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5525173.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6909..8127
                     /gene="rhaA"
                     /locus_tag="OO160_003152"
                     /pseudo
     CDS             6909..8127
                     /gene="rhaA"
                     /locus_tag="OO160_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8369..9193
                     /gene="rhaD"
                     /locus_tag="OO160_003153"
                     /pseudo
     CDS             8369..9193
                     /gene="rhaD"
                     /locus_tag="OO160_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9203..9517
                     /gene="rhaM"
                     /locus_tag="OO160_003154"
     CDS             9203..9517
                     /gene="rhaM"
                     /locus_tag="OO160_003154"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5525174.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9818..10264
                     /locus_tag="OO160_003155"
     CDS             9818..10264
                     /locus_tag="OO160_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5525175.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10275..11726
                     /locus_tag="OO160_003156"
     CDS             10275..11726
                     /locus_tag="OO160_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5525176.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11716..12786
                     /locus_tag="OO160_003157"
     CDS             11716..12786
                     /locus_tag="OO160_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5525177.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12786..>13410
                     /locus_tag="OO160_003158"
     CDS             12786..>13410
                     /locus_tag="OO160_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5525178.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3205 a         3493 c         3538 g         3174 t
ORIGIN      
        1 taaagcgtga ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc
       61 ttgcagaggc gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct
      121 cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg
      181 aaatataggg gcaaatccag ggattaagga agcaggttta tttcaccgta cctaaaattt
      241 cgattttcgc tgctctggct ttgactttac gtgaccagat agaggtaaga attggcacca
      301 gaatcgaagt cacaatgacc gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt
      361 taaacgcagg caccatttct gcaatcagca caggtgtcgc taccgcggcc cctgcggaac
      421 tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatcg gcgataatca
      481 acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc aaaccagtct
      541 gggcaatcac agtcaaatca atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg
      601 tttgcaccgc tttgctgaaa aattctcgta attcagggtc aagattcccc agggcaaagc
      661 ccaccaggaa cggtaatact gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg
      721 cagtgcccag aataatcatc gtcatgagcg gcccggactc caacgacatc aacacaaatg
      781 ccccagcttc ttctttcgta ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg
      841 tcatatccat cgccgccacc agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa
      901 caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt
      961 tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt aattttattg
     1021 acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta atcatcccgt
     1081 tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa
     1141 ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt tttatctgca
     1201 tttccatcac ctgtcggcat atttttaagg gctgatgcgt aattcctcaa tggagcatca
     1261 gtgttgatgg aaacgaagat agacattcat ttaatcaata aaagtgatca aaatcacatc
     1321 aattgaaacg ctgttttatt tttataggac cattgttttt attttggaat tgatcacaaa
     1381 aaaaccaccg ccgttggcga tggttcattt tttaagctga tatgcggcct atacgcctca
     1441 ttgcagcagg cggcaaatga ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag
     1501 ttcaccacgt tccagaactc tttgatgtag tccggacggc ggttctggaa tttcaggtag
     1561 taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc agaaatagct
     1621 tcacccatca gcggagaatc ctggttagca gtagaaacca ccgccagttt atcgcctttc
     1681 agcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc aaattctgct
     1741 ttgaagttat caacggagcc gaagtcacgt tcgatagccg ctttcaggtc accctgcagg
     1801 gtggtgcctt ttttcagacc tttccagaac aggctgtggt tagcgtgacc gccagcgttg
     1861 ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat cagctcttca
     1921 accggcaggt tggcaaattc tggcaggctt tccagcgccg cgttggcgtt gtttacgtag
     1981 gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg cggttccagg
     2041 gcatcgtaag catacggcag ggatggcagg gtatagctca tattcatctc cagtattgtc
     2101 gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat taaatgatat
     2161 tgaaaatgat tatcaatgcc gtacttttcg taagggtatg gttttgcagg aaaatgcccg
     2221 agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcgac aacggcggca
     2281 acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga gatgtgaagc
     2341 gacgaaaaat gatgaggata agaagatgag taacgcgatt acgatgggga tattttggca
     2401 tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg
     2461 gtcatgggaa accatgtggt cagtcggtgg gattgtttcg tggattattc tgccgtgggc
     2521 catcagcgcc ctgttactac cggatttctg ggcgtattac agctcgttta gtctctctac
     2581 gctgctgcct gtttttctgt tcggcgctat gtgggggatc ggtaatatca actatggcct
     2641 gaccatgcgt tatctcggca tgtcgatggg aattggcatc gccattggca ttacgttgat
     2701 tgtcggtacg ctgatgacgc caattatcaa cggcaatttc gatgtgctga ttaacaccga
     2761 aggcggacgc atgacgttac tcggcgttct ggtggcgctg attggcgtag ggattgtaac
     2821 tcgagccggg cagttgaaag agcgcaagat gggcattaaa gccgaagagt tcaatctgaa
     2881 aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccgggatgt cctttgcgat
     2941 gaacgccgca aaaccgatgc atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt
     3001 tgctctgcca agctatgttg tcatcatggg cggcggcgcg atcattaacc tcggtttctg
     3061 ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa
     3121 accgctgatc attcacaatg tgttactctc ggcactgggc gggttgatgt ggtatctgca
     3181 attctttttc tatgcctagg gccacgcccg cattccggcg cagtatgact acatcagttg
     3241 gatgctgcat atgagtttct atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga
     3301 gtggaacaat acaggacgcc gtccggtaac ggtgttgagc ctcggttgtg tggtgattat
     3361 tgtcgccgct aacatcgtcg gcatcggcat ggcgaattaa tctttctgcg aattgagatg
     3421 acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa aatagttact
     3481 atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat gctggagaag
     3541 atattgcgca tgacacactc tgacctgccg cagatattga ttgatggtca ttccagtctg
     3601 ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac aaaatttatc
     3661 cagcgcaaag ggacttttca ggctagccgc cagccgggta atcagcttat ccagcaacgt
     3721 ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca gcaacatcac
     3781 caactgcccg aacagcaact cagccatttc gttagcaaac ggcacatgct gactactttc
     3841 atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca
     3901 gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgct ccctgccagt caagattcag
     3961 cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa cggaagcgta
     4021 ggagtgttta tcgtcagcat gaatataaaa gagatcgcca cgggtaatgc gataaggacg
     4081 atcgttgagt acatgcagac cattaccgcg ccagacaatc accagctcac aaaaatcatg
     4141 tgtatgttcg gcaaagacat cttgcggata acggtcagcc acagcgactg cctgctggtc
     4201 gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg ctacctcggc
     4261 cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt
     4321 attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg tgaccagtta aactctcggc
     4381 gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca gcgataggcg atgtcagtga
     4441 cgctggcctc gctgtggcgt agtagatgtc gggctttcat cagtcgcagg cggttcaggt
     4501 atcgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta cgcagtgaaa
     4561 gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc
     4621 aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc aaactgcttt
     4681 tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc gagggtaaat
     4741 cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc tgcaatacgc
     4801 tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc agcaactgat
     4861 tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg gtcagacaca
     4921 gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag accgtgccac
     4981 cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt tcgacaatca
     5041 caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc cggggttcta
     5101 tcgccacgga cgcgttacaa gacggaaaaa aatccacact atgtaatacg gtcatactgg
     5161 cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct taccttaaat
     5221 tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg aattttcagg
     5281 aaatgcggtg agcatcacat caccacaatt cagcaaattg tgaacatcat cacgttcatc
     5341 tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcgc gaattcaggc
     5401 gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt tcgcaattgt
     5461 gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa
     5521 tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atgggctgca tagccaaaac
     5581 ggttatgtca cctgggatgt ggatagcctg gaaagtgcca ttcgccttgg attaaacaag
     5641 gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg cgtggacttt
     5701 gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc
     5761 accaatggcc tgatgccgca ggcacaacag caactcggca aacgcgatat ttatcagcgt
     5821 agcggcatcc agtttctacc cttcaatacg ctttatcagt tgcgtgcgct gacggagcaa
     5881 caacctgaac ttattccaca cattgctcac gctctgctga tgccggatta cttcagttat
     5941 cgcctgaccg gcaagatgaa ctgggaatat accaatgcca ccaccacaca actggtcaat
     6001 atcaatagcg acgactggga cgagtcgcta ctggcgtgga gcggggccaa caaagcctgg
     6061 tttggtcgcc cgacgcatcc gggtaatgtc attgggcact ggatttgccc gcagggtaat
     6121 gagattccgg tggtcgccgt tgccagccat gataccgcca gcgcggttat cgcctcgccg
     6181 ttaaacggtt cacgcgccgc ttatctctct tctggcacct ggtcattgat gggcttcgaa
     6241 agccagacgc catttaccaa tgacacggcg ctggcagcca acatcaccaa tgaaggcggg
     6301 gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct tcagcgagtg
     6361 cttcaggaac ggcaaatcaa cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg
     6421 gcctgccgct tcatcatcaa tcccaatgac gatcgcttta ttaatcctga cgagatgtgc
     6481 agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga tcccgggaag tgatgctgaa
     6541 ctggcgcgct gtattttcga cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg
     6601 gcgcagctgc gcggtgaaga tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac
     6661 acgctgctca accagctatg cgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt
     6721 gaagcctcaa cgctcggcaa tatcggcatc gagttaatga cgctggatga actcaacaat
     6781 gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt tacccctaat
     6841 cctgacagtg aaattgccca ctatgtggcg cagattcact ctacacgaca gacaaaggag
     6901 ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg
     6961 cggtggggat tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc
     7021 actgctggca gggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg ctgaccgggg
     7081 ggattcaggc cacaggcaat tatccgggca aagcgcgtaa tgccagtgag ctacgtgccg
     7141 atctggaaca ggctatgcgg ctgattccgg ggccgaaacg gcttaattta catgccatct
     7201 atctggaatc agatacgcca gtctcgcgcg accagatcaa accagagcac ttcaaaaact
     7261 gggttgaatg ggcgaaagcc aatcagctcg gtctggattt taacccctcc tgcttttcgc
     7321 atccgctaag cgccgatggc tttacgctct cgcatcccga cgacagcatt cgccagttct
     7381 ggattgatca ctgcaaagcc agccgtcgcg tttcggccta ttttggcgag caactcggca
     7441 caccatcggt gatgaacatc tggatcccgg atggtatgaa agatatcacc gttgaccgtc
     7501 tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt gatcagcgag aagctaaacc
     7561 ctgcgcacca tatcgacgcc gttgagagca aattgtttgg cattggcgca gagagctaca
     7621 cggttggctc caatgagttt tacatggggt atgccaccag ccgccagact gcgctgtgcc
     7681 tggacgccgg gcacttccac ccgactgaag taatttccga caagatttcc gccgccatgc
     7741 tgtatgtacc gcagttgctg ctgcacgtca gccgtccggt tcgctgggac agcgatcacg
     7801 tagtgctgct ggatgatgaa acccaggcaa ttgccagtga gattgtgcgt cacgatctgt
     7861 ttgaccgggt gcatatcggc cttgacttct tcgatgcctc tatcaaccgc attgccgcgt
     7921 gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga ttacactgcg
     7981 cgtctggcac tgctggaaga gcagaaatcg ttgccgcggc aggcggtctg ggaaatgtat
     8041 tgccaacatc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg ggcttatgag
     8101 aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcacgagc gccttatccg
     8161 gcctacgggt cgaatgtcca tgcgggctgt caccctcacc ccggccctct ccctgaaagg
     8221 gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacaccgctc cggacagtcc
     8281 cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat gtctaaacaa
     8341 ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct ggtttgtcca
     8401 gggaatgatc aaagccacca ccgatgccta gctgaaaggc tgggacgagc gcaacggcgg
     8461 caacctgacg ctgcgcctgg atgacgccga tatcgcgctt tatcacgaca acttccaccc
     8521 acaaccgcgc tatattccgc tcagccaacc catgccttta ctggcaaata cgccgtttat
     8581 tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc gatcctgcag ctaacttagg
     8641 cgtcgtaaaa gtcgacagcg atggcgcggg ctaccatatt ctctgggggt taaccaacga
     8701 agccgttcca acttctgaac ttccggctca ctttctctcc cactgcgagc gcattaaagc
     8761 caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga tcgccctcac
     8821 ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag gcagcaccga
     8881 atgtctggta gtattcccgg atggcgttgg cattctgccg tggatggtgc ccggcacgga
     8941 cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt tgtggccctt
     9001 ccacggtgtg ttcggttccg gaccgacgct ggatgaagcc tttggtttaa tcgacaccgc
     9061 agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga aacagaccat
     9121 cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac tagccagtgc
     9181 gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg taaaccccga
     9241 cgcccacgaa gagtatcagc gtcggcataa tcccatctgg ccggaactgg aagcagtgtt
     9301 gaaatctcac ggtgtgcata actacgccat ttacctcgac aaagcgcgta atctgctgtt
     9361 tgccatggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta ctgatgtttg
     9421 ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccagata acagcccggt
     9481 gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc cggagatgcc
     9541 tccggcacaa tattttaaat ttcgatttga aaaattaaac atattacatc tcccggtaag
     9601 ttatatttcc ctgatacatt gtgagtaaat cacaaaaata atgaataacc cattaatgat
     9661 tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctga cctttcactc
     9721 tgtctcaaat tgtcattgtt taccgacata tcgcctctca tcacttaatc gctccaccat
     9781 tacaattgat taaaatatat tttatggagt ccgattaatg gcagctctta ctgcaagctg
     9841 tattgacctg aatattcagg gcaatggcgc ttattccgtt ctgaagcagt tggcgacaat
     9901 agcgttacaa aacggtttta tcaccgactc acaccagttc ctgcaaaccc tgctgctgcg
     9961 cgaaaaaatg cactctactg gatttggttc cggtgtcgca gtgccgcacg gtaaaagcgc
    10021 ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg caggctattg actggaaagc
    10081 cagcgatggc gaagacgtca attgctggat ctgcctcggc gtgccgcaaa gcggcgaaga
    10141 ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt attcaccagg aatttattca
    10201 tcaactgcaa cagggcgata ccgaccaggt gctggccttg ttaaatcaaa ccctcagctc
    10261 ataaggaagt ggcgatggag tcatccttac gtattgtcgc gatcaccaac tgccccgccg
    10321 ggatcgctca cacctacatg gtggcggaag ccctggaaca gaaagcccgt tctctcggcc
    10381 ataccataaa agtggaaact caagggtcca gtggcgttga aaaccgctta tccagcgaag
    10441 agattgccgc tgccgattac gtcattcttg ctaccgggcg tggcctgagc ggtgatgatc
    10501 gcgcgcggtt tgccgggaag aaagtttatg agattgccat ctccctggcg ttgaaaaata
    10561 tcgaccagat tttcagcgaa ttaccgacaa actcgcagct ttttgccgca gatagcggcg
    10621 tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat gagccacctg atggcgggcg
    10681 tctctgcggc gctgccgttt gtcatcggcg gtggtatcct ggtggcgctt gccaacatgc
    10741 tggtgcagtt cggtttgccg tataccgata tgtcgaaagg cgcgccgtcg tttacctggg
    10801 tagttgaatc catcggttac cttggcttca cctttatgat ccccatcatg ggcgcttata
    10861 ttgcctcgtc gattgccgat aaaccggctt tcgctccggc gtttctggtt tgctatctgg
    10921 cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg attcctcggc gcggtggtgc
    10981 tggggctggc gattggctat ttcgtcttct ggttccgcaa agtgcgcctt ggcaaagcat
    11041 tacaaccgct gctcggttca atgctgatcc cgttcgttac tctgctggtt ttcggcgtgc
    11101 tcacttacta cgttatcggg ccggtgatgt ccgacctcat gggcgggctg ctccacttcc
    11161 tgaataccat tcctccatca atgaagtttg cggcagcgtt tctggtcggt gcgatgctgg
    11221 cgttcgatat gggcggcccc atcaataaaa ccgcctggtt cttctgcttc tcactgctgg
    11281 aaaaacacat ttatgactgg tacgccatcg tcggcgttgt cgcgttgatg ccgcccgttg
    11341 cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa aaagaggctg
    11401 ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctata ccttacgctc
    11461 tggcagctcc gttgccgatg attaccgcca atacgctggc gggcggcatt accggcgtgc
    11521 tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt gacccgctga
    11581 tcggcctgat gtcgccggta ggttcgtttt atctggtgct ggcgattggc ctcgcgctga
    11641 acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg aaagccgcgc
    11701 aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc cagcgccgtt
    11761 agcggcgatg aacaggaagt tcgcgacatc ctgataaaca cgctggaacc ttgcgttaat
    11821 gaaatcacct ttgatggtct gggcagcttt gttgcccgta agggaaataa aggtccaaaa
    11881 gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat cgacgagagc
    11941 ggttttctgc gtttcaccac cattggtggc tggtggaatc agtcgatgct caaccatcgg
    12001 gtaaccatac gcacacacaa gggagtgaaa atccctggtg tgattggttt cgtcgcgcct
    12061 catgcgttaa cggaaaagca aaggcaacag ccgctgtcat ttgatgagat gttcattgat
    12121 attggcgcaa acagtcgcga tgaggtggaa aaacgcggtg tggcgatggg cgattttatt
    12181 agcccggaag ccaattttgc ctgctggggc gaagataaag tggtcggcaa ggcgctggat
    12241 aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga cggtgaataa tcccgaaatt
    12301 acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc tacgcggggc acaaacctcg
    12361 gcggaacaca ttaaaccgga tgtagtgatc gtgctggata ccgctgtcgc aggcgatgtt
    12421 ccgggcattg ataacattaa atacccgctg aaactgggcc aggggccggg gctgatgctg
    12481 tttgacaagc gctacttccc caaccagaaa ctggtagcag cgttaaaaaa ctgtgccgca
    12541 cataacgatt taccgctgca attttccacc atgaaaaccg gagcgacgga tggcgggcgc
    12601 tacaacgtga tgggcggcgg gcgtccggtt gttgcgctgt gcctgccaac ccgctatctg
    12661 cacgctaaca gcggcatgat ttcaaaagcc gattacgatg ctctgctcac gttgatacgg
    12721 ggttttctga cgaccttaac cgcggagaaa gtcaacgcgt ttagccagtt ccgtcaggtg
    12781 gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa cagccgcgca
    12841 cgcctggtga gctggcgcaa cagactggcg tttcaggcag gaccatcctg cgtgatattg
    12901 actatctcaa ctttaccctt aacggcaaag cccgcatttt cgccagtggc agtgcgggct
    12961 atcagctgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag cacgataacg
    13021 acgatcggct gctggcgctg ttattactga atactttcac tccccgtgcg caactcgcct
    13081 cggcgcttaa tttgccagaa acgtgggtag cagagcgtct gccccagtta aaacagcgtt
    13141 atgaacgcac ttgttgcctg gccagccgcc ctggtttggg ccatttcatt gatgagacag
    13201 aagagaaact cgttatcttg ctggcgaact tgctgcgcaa agatccgttt ttaattccgc
    13261 tggcgggcat aacacgagac aaccttcagc atttatccac ggcctgcgac aaccaacacc
    13321 gctggccgct catacagggt gattatctct ccagcctgat tctggcgatt tacgccctgc
    13381 gtaatcaact gaccgatgag tggccgcaat
//
LOCUS       DAJTNP010000095        13100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTNP010000095 DAJTNP010000000
VERSION     DAJTNP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..893)
                     /locus_tag="OO160_003159"
     CDS             complement(<1..893)
                     /locus_tag="OO160_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5525179.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="OO160_003160"
     CDS             1134..1934
                     /locus_tag="OO160_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5525180.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO160_003161"
     CDS             2012..2782
                     /locus_tag="OO160_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5525181.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO160_003162"
     CDS             complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO160_003162"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5525182.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO160_003163"
     CDS             complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO160_003163"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5525183.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5037..5759
                     /locus_tag="OO160_003164"
     CDS             5037..5759
                     /locus_tag="OO160_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5525184.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO160_003165"
     CDS             complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO160_003165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5525185.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO160_003166"
     CDS             6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO160_003166"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5525186.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7152..7228
                     /locus_tag="OO160_003167"
     tRNA            7152..7228
                     /locus_tag="OO160_003167"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7186..7188,aa:Asp,seq:gtc)
     gene            complement(7359..8405)
                     /locus_tag="OO160_003168"
     CDS             complement(7359..8405)
                     /locus_tag="OO160_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5525187.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO160_003169"
                     /pseudo
     CDS             complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO160_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO160_003170"
     CDS             complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO160_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5525188.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO160_003171"
     CDS             complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO160_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5525189.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11365..12195)
                     /locus_tag="OO160_003172"
                     /pseudo
     CDS             complement(11365..12195)
                     /locus_tag="OO160_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12182..13096)
                     /gene="tagK"
                     /locus_tag="OO160_003173"
                     /pseudo
     CDS             complement(12182..13096)
                     /gene="tagK"
                     /locus_tag="OO160_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3395 c         3485 g         3213 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc
     3061 gcgcagtttg ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct
     3121 tgaaagcgtg tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc
     3181 aaccagcgtc gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc
     3241 tgcatgcttc tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt
     3301 cacgccagcg ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt
     3361 cgccatttca accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt
     3421 tggcagacgt acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt
     3481 aggcacgtac tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc
     3541 acgcgctttg ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc
     3601 tacggtcaga agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc
     3661 ggcagttgtt gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc
     3721 ataattgcgc cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt
     3781 tgcgtgagga tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt
     3841 acgtttttta acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc
     3901 gtggagatag ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc
     3961 cggaattccc atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat
     4021 agacgtccca tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc
     4081 agctgcagaa aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag
     4141 caggacagag gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa
     4201 gacgaccgat cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct
     4261 ttctttgacc ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta
     4321 attccattaa ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt
     4381 ttcagaatta cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa
     4441 taatcattta tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac
     4501 gtatattcat gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt
     4561 gattgataca tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc
     4621 gtgtcgccgc aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc
     4681 ggtgtggtaa ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact
     4741 acctgtgttg ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac
     4801 ttttccacca gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg
     4861 gcctccggtt gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga
     4921 tcgacaatca ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag
     4981 gcgggaatac tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga
     5041 aaccggcaag agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg
     5101 gagagcttta tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg
     5161 gttttcatct gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg
     5221 tttctcatca agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac
     5281 ttcatcttcc ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt
     5341 ggtgcaccga tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct
     5401 ggctggtgat tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg
     5461 tattgcgcaa aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact
     5521 ggctctcttt gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga
     5581 acaaacacgg aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta
     5641 ttgttgcccg gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca
     5701 agccacgaat tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa
     5761 cttcgacttg gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt
     5821 catcacagcg ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt
     5881 gatgctgccc caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt
     5941 tgtctgctgt tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc
     6001 ggacatactg gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg
     6061 cgacaatagc ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa
     6121 aggttttctc gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac
     6181 ccagacacga accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg
     6241 taattgaatc gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa
     6301 ttacacgcca gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact
     6361 atgaaggcca caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg
     6421 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
     6481 tacatggtat tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
     6541 agtttatgga ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag
     6601 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct
     6661 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc
     6721 tcgatgcgct atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
     6781 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
     6841 tggcttttgc gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca
     6901 ttgtacgtca ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac
     6961 atgaagcccg tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa
     7021 tacatgtaaa aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa
     7081 gatgagaaaa atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg
     7141 gaacacaacg cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc
     7201 gcgggttcga gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg
     7261 ggctttcgtc gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt
     7321 aaaaaatggc cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc
     7381 tccagcagtt ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt
     7441 tctgcctggc gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga
     7501 cggatgacac gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg
     7561 ccggtcatcg gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc
     7621 atctgttcag tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg
     7681 atctccggcg tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg
     7741 gagaagtccg gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg
     7801 gtctctgctg cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc
     7861 agttgctggc gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa
     7921 cgctccacat cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga
     7981 atatccgcca ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc
     8041 gcagcatctt cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg
     8101 tacgtggagc acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc
     8161 tgctggatac ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc
     8221 agacgatggg cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa
     8281 tcgccatatt gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga
     8341 tcgtactcca gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt
     8401 tccatttaat tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgctggactc
     8461 acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct
     8521 ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg
     8581 tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag
     8641 ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc
     8701 gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc cccacagcac gaggctgtac
     8761 agactgatgt gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag
     8821 gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc
     8881 gccggaacgg tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc
     8941 cagggggggg cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc
     9001 tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg
     9061 tattaatgga aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac
     9121 tgatgccgga aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga
     9181 ccagtagtcc ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc
     9241 tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc
     9301 gcaacgcctg gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata
     9361 actggcggat caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg
     9421 gcgggcggat aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc
     9481 ggccattgcg ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga
     9541 tgtggcgcag attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt
     9601 ccgagcccgt atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg
     9661 gctcccggcg cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt
     9721 agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga
     9781 ccgagaacac ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg
     9841 tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac
     9901 agaacaggct gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt
     9961 tcagcaggat aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc
    10021 ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat
    10081 gaccgtgaaa cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt
    10141 acataacggg ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag
    10201 ccaggggcgc gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct
    10261 cgccgcagag ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac
    10321 caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc
    10381 ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg
    10441 gccacagcgt tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa
    10501 tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt
    10561 ccgtatcggg atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc
    10621 tgaccacttc cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg
    10681 tgcgggcggt caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat
    10741 ccgttccctg catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc
    10801 ccgtttcccg cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg
    10861 gtctctctct gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta
    10921 cccggaatgc agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg
    10981 tgttatggcc actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt
    11041 tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact
    11101 tgtgctcata cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg
    11161 ccgcgtacgg ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa
    11221 atgacaaatc ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt
    11281 cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat
    11341 tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca
    11401 ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag
    11461 cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag
    11521 ggagtcggca gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc
    11581 gccattgttc agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg
    11641 cggcgatttc agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat
    11701 cagttcgagt accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc
    11761 attccactgc ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc
    11821 ttcctgcgct tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc
    11881 gggcggcggg agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat
    11941 ctcacagcgg atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg
    12001 ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg
    12061 aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat
    12121 ctgaatgccc tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc
    12181 gttattgttt gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt
    12241 caatgccaat gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg
    12301 tttccggggc gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag
    12361 gccagatagt ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta
    12421 acccaccagt gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat
    12481 ccagatcctg cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat
    12541 cttgttcggg agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc
    12601 ctttttccgc accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat
    12661 tttgtgatgt cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt
    12721 ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct caggttcgga gatgatggat
    12781 tgccatgtac aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac
    12841 gcatacggtg atgaggttcc acaatctggt cgtttaccgt acagcaatat tcggtagact
    12901 gattgataat ccaccatgct tcttcgtggc gggtcagagt gagggcaatc tttacatcat
    12961 cattcacctt aaaaacagga atccagggag aggtaacggt gaagataatt tgtgttcctt
    13021 cagtcgcgta aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg agtccccacg
    13081 taatatttgg tttcattgtg
//
LOCUS       DAJTNP010000096        12996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTNP010000096 DAJTNP010000000
VERSION     DAJTNP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..615
                     /gene="mxiE"
                     /locus_tag="OO160_003174"
     CDS             <1..615
                     /gene="mxiE"
                     /locus_tag="OO160_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5525190.1"
                     /translation="VRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAE
                     KCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRT
                     IEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTI
                     TSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            602..2302
                     /gene="mxiD"
                     /locus_tag="OO160_003175"
     CDS             602..2302
                     /gene="mxiD"
                     /locus_tag="OO160_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5525191.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            2321..3388
                     /gene="mxiC"
                     /locus_tag="OO160_003176"
     CDS             2321..3388
                     /gene="mxiC"
                     /locus_tag="OO160_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5525192.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            3401..5461
                     /gene="mxiA"
                     /locus_tag="OO160_003177"
                     /gene_synonym="virH"
     CDS             3401..5461
                     /gene="mxiA"
                     /locus_tag="OO160_003177"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5525193.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            5474..5875
                     /gene="spa15"
                     /locus_tag="OO160_003178"
                     /gene_synonym="spaK"
     CDS             5474..5875
                     /gene="spa15"
                     /locus_tag="OO160_003178"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5525194.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            5879..7171
                     /gene="spaL"
                     /locus_tag="OO160_003179"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5879..7171
                     /gene="spaL"
                     /locus_tag="OO160_003179"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5525195.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            7264..7602
                     /gene="spa13"
                     /locus_tag="OO160_003180"
                     /gene_synonym="spaM"
     CDS             7264..7602
                     /gene="spa13"
                     /locus_tag="OO160_003180"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5525196.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            7589..8467
                     /gene="spa32"
                     /locus_tag="OO160_003181"
     CDS             7589..8467
                     /gene="spa32"
                     /locus_tag="OO160_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5525197.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            8461..9342
                     /gene="spa33"
                     /locus_tag="OO160_003182"
                     /gene_synonym="spaO"
     CDS             8461..9342
                     /gene="spa33"
                     /locus_tag="OO160_003182"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5525198.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            9347..9997
                     /gene="spa24"
                     /locus_tag="OO160_003183"
                     /gene_synonym="spaP"
     CDS             9347..9997
                     /gene="spa24"
                     /locus_tag="OO160_003183"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5525199.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            10012..10272
                     /gene="spaQ"
                     /locus_tag="OO160_003184"
     CDS             10012..10272
                     /gene="spaQ"
                     /locus_tag="OO160_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5525200.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            10272..11042
                     /gene="spa29"
                     /locus_tag="OO160_003185"
                     /gene_synonym="spaR"
     CDS             10272..11042
                     /gene="spa29"
                     /locus_tag="OO160_003185"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5525201.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            11049..12077
                     /gene="spa40"
                     /locus_tag="OO160_003186"
                     /gene_synonym="spaS"
     CDS             11049..12077
                     /gene="spa40"
                     /locus_tag="OO160_003186"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5525202.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            12090..12332
                     /locus_tag="OO160_003187"
     CDS             12090..12332
                     /locus_tag="OO160_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525203.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         4170 a         1851 c         2533 g         4442 t
ORIGIN      
        1 gtccgaaatc aaaatataaa attcagtgat aatgtgaact atcattatcg ctttaacatt
       61 aatagttgtg caaaatttct tgctttttgg gactatttca gtggtgctct agtagagcat
      121 agccatgctg aaaaatgtat tcatttctat catgagaatg atcttcggga tagttgtaat
      181 accgaatcga tgcttgataa gttaatgtta agattcatct tttcatctga ccagaatgta
      241 agtaatgcac tggctatgat acgaatgact gagtcatatc atttggttct ttatcttctg
      301 agaactattg agaaagaaaa ggaggtaaga ataaagagtc tgacagagca ttatggtgtg
      361 tcggaggctt attttagatc tttatgtaga aaagctttag gggcaaaagt caaagaacaa
      421 ctgaacactt ggcggttagt aaatggttta ttagatgtat ttttgcacaa tcaaacaatc
      481 acttcagctg ccatgaataa tgggtatgca tcaacatccc atttttcaaa tgagataaaa
      541 acacgactag gcttttctgc aagagaatta tcgaatatta cttttttagt gaaaaaaata
      601 aatgaaaaaa tttaatatta aatctttgac tctcttgatt gtattgttac cattgatagt
      661 taatgctaat aatattgact ctcatttatt agaacaaaat gacatagcga aatatgttgc
      721 tcaaagtgat acggtaggat ctttttttga acgtttttct gcgttattaa attatcctat
      781 tgttgttagc aaacaagctg caaaaaagag aatatctggg gagtttgatt tatctaatcc
      841 agaagaaatg cttgaaaaac tgacattact tgtgggactc atatggtata aagatggaaa
      901 tgcattatac atatatgact ctggagaatt aataagcaaa gttattttgc tggagaatat
      961 atcacttaat tatcttattc agtatctgaa ggatgctaac ctttatgatc acagatatcc
     1021 tataagagga aacattagtg ataaaacatt ctatatatct ggtcctccgg cattggttga
     1081 actggtagct aatacagcaa ctttgcttga taagcaggtt tcttctattg ggacagataa
     1141 agttaatttt ggggttataa aattaaaaaa tacatttgtt agtgacagaa cttataatat
     1201 gagaggggag gatattgtca tcccaggggt ggctactgtt gtagaacgtt tattaaataa
     1261 tggtaaggct ctgagtaaca gacaagccca aaatgatccg atgccaccct ttaatattac
     1321 acagaaagta tctgaggata gtaatgactt ttcatttagt tcagttacca atagttctat
     1381 attagaggat gtatcactta ttgcatatcc agaaaccaat tctatattag tcaaaggtaa
     1441 tgatcagcaa atacagatta ttcgcgatat tatcactcag ttagatatag ccaaacgaca
     1501 tatagaatta tcattatgga taatcgatat tgataaatct gaattaaata accttggtgt
     1561 taattggcaa ggaacggctt catttggtga ttcgtttggt gcgtcattta atatgagctc
     1621 atcagcatcg ataagcactt tggacggcaa taagtttatt gcttcagtta tggcattaaa
     1681 tcagaaaaag aaagcaaatg tagtttcaag gccggtgatt ttgacacaag agaatattcc
     1741 ggctatattt gataataaca ggacttttta tgtgtcatta gttggtgagc gcaattcttc
     1801 gttggagcat gttacgtatg gaacattgat aaatgttata cctcgctttt catctcgggg
     1861 acaaattgag atgtctttaa cgatagagga tggaacagga aatagtcagt caaattacaa
     1921 ttataataat gaaaatacat ctgtccttcc agaagttgga aggaccaaaa tcagtacaat
     1981 agcgagagtt ccacaaggca aaagtttgtt gataggtggt tatacgcatg aaacgaactc
     2041 taacgaaata atatctattc cttttctctc ctctattcct gtaatcggta atgtgttcaa
     2101 gtataaaaca agtaatatca gtaatattgt aagggttttt ctgattcaac cccgtgagat
     2161 aaaggagtca agttattata atactgcaga atataaaagc ctcatatctg aaagagaaat
     2221 acaaaagact acacaaataa tcccatctga aactactttg ctggaagacg aaaaatcatt
     2281 ggtttcatac ttaaattact aactataaag taggtgatgt atgcttgatg ttaaaaatac
     2341 aggagttttt agctctgcat tcattgataa gttgaatgca atgacaaatt cagatgatgg
     2401 agatgagact gctgatgcag agcttgattc tggcttggct aatagcaagt atattgactc
     2461 atctgatgag atggcttccg ctctttcgtc atttataaac agaagagacc ttgagaaact
     2521 gaaaggaaca aatagtgata gtcaggaacg tattttagat ggggaagaag atgaaattaa
     2581 tcacaagatt tttgatttaa agagaacgtt aaaagataac cttcctctag atcgggattt
     2641 tatagacaga ctaaagagat attttaaaga tccaagtgat caagtcttag cattaaggga
     2701 acttttgaat gaaaaagatc ttactgctga acaagtcgaa ttattaacta aaattattaa
     2761 tgagataata tcaggtagtg aaaaaagtgt taatgctgga ataaattcag ctatacaggc
     2821 taaattattt ggcaacaaaa tgaaacttga accacagctt ttgcgtgcat gttatcgtgg
     2881 ttttatcatg gggaacatat caacaacaga tcagtatata gaatggcttg gtaattttgg
     2941 ttttaatcac agacatacaa ttgtgaattt tgtagagcag tcactgattg tagacatgga
     3001 ttctgagaaa ccgagctgta atgcttatga gtttggtttt gtgttatcta aattaattgc
     3061 aattaagatg attagaactt cagacgtaat ttttatgaag aaactggaat cctcaagctt
     3121 gctaaaagat ggcagtttaa gtgcagagca gctattgcta actttattat atatttttca
     3181 atatccaagt gaaagtgagc aaattcttac ttctgttata gaagtatcac gagccagtca
     3241 tgaggattct gtagtgtatc aaacatatct atcttctgtt aatgaaagtc ctcatgatat
     3301 atttaaaagt gaaagtgaaa gagaaattgc gatcaatatt ctacgagagc ttgtcacaag
     3361 tgcatacaag aaagagcttt ctagataaca ggagataaaa gtgatccagt cttttcttaa
     3421 gcaagtaagt actaagcctg aattaattat attagtgctt atggttatga tcattgctat
     3481 gttgatcata ccattaccta cataccttgt tgatttttta atagggctta atattgtact
     3541 tgctattctc gtttttatgg gatcatttta tattgaaaga atattgagtt tttctacatt
     3601 cccttcggtt ttgttgataa cgacattatt tcgtctcgct ttatcaataa gtactagtcg
     3661 actaatttta gttgatgcgg atgcgggtaa aattattact acatttggcc agtttgtcat
     3721 tggtgatagt ctggctgtag gttttgttat tttttcaata gtaactgttg tgcagttcat
     3781 tgttataaca aaaggatctg aaagggttgc ggaggtagct gctcgctttt ctcttgacgg
     3841 tatgccgggg aaacaaatga gcattgatgc ggatttaaag gctggaatta ttgatgccgc
     3901 aggagctaaa gaaagacgca gtattctgga acgtgagagt caattgtatg gttcatttga
     3961 tggagcaatg aagttcatta aaggtgatgc aattgctggc atcattatta tttttgtcaa
     4021 tttaatagga ggtatttctg ttggtatgag tcaacatgga atgtccctat ctggtgcatt
     4081 atctacttat accatactaa ctattggtga cggattggtc tctcagattc ctgctctgtt
     4141 aatttctata agtgcaggat ttatcgttac aagagttaat ggtgatagcg ataatatggg
     4201 acgtaacata atgtcccaga tatttgggaa tccttttgtt ctcattgtta catcagctct
     4261 tgccttggcg atagggatgt tgccaggttt tccgtttttt gtttttttcc tgatagcagt
     4321 tactttgacg gctttattct attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga
     4381 gtctgattct agtggctata ctggtacatt tgatattgat aatagccatg actcatcttt
     4441 ggcaatgata gaaaatctgg atgctattag ttcagaaacc gttcctttaa ttttattatt
     4501 tgccgaaaat aagataaatg caaatgatat ggaaggtctt attgaaagga taagaagtca
     4561 gttctttatt gattatggtg tcaggcttcc aactatttta tatagaacaa gcaatgagct
     4621 taaggtcgat gatattgttt tgttaataaa tgaggtgcgt gccgatagtt ttaatatata
     4681 ttttgacaaa gtgtgtatta cagatgaaaa tggagatata gatgctctag gtattcctgt
     4741 tgtttcaact tcatataatg aacgtgttat ttcttgggta gacgtttcat atacagaaaa
     4801 tctaactaat attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc
     4861 acaagcttta ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt
     4921 agatcagttt gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat
     4981 acagagaatt tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt
     5041 aaaacttatt atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt
     5101 agttgaacat gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg
     5161 tgagattaaa gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag
     5221 gcaaacctct ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga
     5281 aactttagca catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt
     5341 atcagtagat atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact
     5401 cgttatatcg tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta
     5461 gtgaggttta aatatgagta acattaattt agttcaatta gttagagata gtcttttcac
     5521 gattggttgt cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc
     5581 tttggatagt atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc
     5641 taattttgat gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt
     5701 aatgttaatg aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga
     5761 gtacttacag ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc
     5821 agagattctg catgagtttt atcagaggat ggaaatctta aatggggtct tataatcaat
     5881 gagctataca aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt
     5941 ggaaacaagt cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga
     6001 aagtaatgaa attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt
     6061 aagcttgatt ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca
     6121 gtttcttcat acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga
     6181 ggttactgat aagtttgctg ttacagataa cagtgaaatt ctttatcgac ctgtagataa
     6241 tgctcctccg ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa
     6301 ggttattgat tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc
     6361 tggttgtggc aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata
     6421 tgttattggg ttaattggtg agcgaggtcg agaggttact gaaacggttg attatttgaa
     6481 aaactctgag aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt
     6541 tgatcgttgt aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg
     6601 acataaagta gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt
     6661 ggccttagcc gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag
     6721 cttacccaga cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt
     6781 ttacactgtt cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc
     6841 cattttagat ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc
     6901 aattgattcc ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg
     6961 tattatggcc gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat
     7021 tgactttggt gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat
     7081 atctgttgtt gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac
     7141 aatggagctt attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac
     7201 ggtctgttaa attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga
     7261 gatgtggagg cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa
     7321 cctgtaggcg ctcagagtgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta
     7381 aaaaagcata ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa
     7441 attcaaaaag atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt
     7501 aaaatatgta aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt
     7561 gatgagttat cacaagagga tatgaagtat ggcattagat aatataaacc taaattttag
     7621 tagtgacaaa cagatagaaa aatgtgagaa actatcttca atagataata ttgatagtct
     7681 cgttttgaag aagaagagga aggttgaaat tcctgagtac tctttaattg catctaacta
     7741 ttttactatt gataagcact ttgaacataa gcatgataaa ggagaaattt atagtggcat
     7801 taaaaatgcg ttcgaactta gaaacgaacg agcgacatat tctgatattc cagaatcaat
     7861 ggccattaaa gaaaatattt tgataccaga tcaagatatc aaagcaaggg aaaaaataaa
     7921 tatcggcgat atgaggggga tcttttcata taataagagt ggaaatgcag acaagaactt
     7981 cgaaagaagt catacttctt ctgtaaaccc tgataatctg ctagaatctg ataatagaaa
     8041 tggtcaaatt ggtttaaaaa atcatagctt gtctattgat aagaatattg ctgacatcat
     8101 ttctttacta aatggaagtg ttgctaaatc atttgagctg cctgtaatga ataaaaatac
     8161 tgcagacata accccatcca tgtcattgca agaaaaatca atagttgaaa atgataaaaa
     8221 tgtttttcaa aaaaatagtg aaatgactta ccactttaaa cagtgggggg ctggacattc
     8281 tgttagtatt tcagtggagt ctggttcttt tgttctaaaa ccgtcagatc aatttgtagg
     8341 aaataaactt gacttaattt tgaaacaaga tgctgagggt aattacagat ttgatagcag
     8401 tcaacataat aaggggaata aaaataatag tacaggatat aatgaacaga gtgaagaaga
     8461 atgctaagaa ttaaacattt tgacgctaac gaaaaactac agattttata tgcaaagcaa
     8521 ctctgcgagc gtttttcgat tcagacattc aaaaataaat ttacaggcag tgaaagttta
     8581 gtcactctta cttccgtgtg tggggattgg gtaattcgta ttgatacatt atcttttttg
     8641 aaaaaaaaat acgaggtatt ttcaggattt tctacacaag aatctttact gcatttatca
     8701 aaatgtgtct ttatagagtc gtcatctgta ttttcgattc cagaactgtc tgataagatt
     8761 actttccgga tcacgaatga aatccagtat gcaactactg ggagtcattt atgctgtttt
     8821 tcctcttctt taggtattat ttattttgac aagatgccgg tattacgtaa tcaagtttct
     8881 cttgactcat tgcatcatct tttagagttt tgcttaggtt catctaatgt aaggctggct
     8941 actttaaaaa gaattcgcac tggtgatata atcatagttc agaaacttta taatttatta
     9001 ttgtgtaatc aagttattat tggggattat attgtgaatg ataataatga ggcaaaaatt
     9061 aatctgtcag aaagtaatgg tgagtcagaa cacacagaag tttctttggc attattcaat
     9121 tatgatgata tcaatgtaaa agtggacttt attcttttag aaaaaaatat gacaatcaat
     9181 gaactaaaaa tgtatgtaga aaacgaatta ttcaagtttc ccgatgacat agttaaacat
     9241 gtaaatatta aagtaaatgg ttctttggtt gggcatgggg aacttgtttc tattgaggat
     9301 ggttatggta tcgagattag ttcttggatg gtaaaggagt aatctcatgc tgagtgacat
     9361 gtccctcatc gctacacttt ctttttttac gctattacca tttttagttg ctgctggtac
     9421 ctgttatata aagttttcta ttgtttttgt aatggtacga aatgcacttg gtttgcagca
     9481 ggttccatca aatatgactc tcaatggtat tgcattgatt atggctttgt tcgtgatgaa
     9541 acccattatt gaagctggtt atgaaaatta tctaaatggc cctcaaaaat ttgatacaat
     9601 atctgatatt gtccgttttt ctgattctgg cttgatggaa tataagcaat acttgaaaaa
     9661 gcatacagat cttgagttag cacgtttttt ccaaaggtct gaagaagaaa atgctgattt
     9721 aaagagtgca gagaataatg attattcatt gttttccctc ttgcctgcat atgcgctaag
     9781 tgaaatcaaa gatgcgttca aaataggttt ttatttatat ttgccatttg ttgttgttga
     9841 tcttgttatt tcaagcattt tacttgcact tggtatgatg atgatgagtc caatcacaat
     9901 atcagtacca ataaaacttg tattatttgt tgcgcttgat gggtggggta tcctgtctaa
     9961 ggccttgatt gaacaatata tcaatattcc tgcttagatg atggaggtaa tgtgtctgat
    10021 atagtttata tgggtaataa ggctctttat ttaatcctta tcttttcctt atggccagta
    10081 ggtatagcta cggttattgg attatctatt ggtttgttac agacagtgac tcaactccaa
    10141 gagcagacac ttccttttgg tataaagctt ataggtgtct caatatcttt gctacttctt
    10201 tctggatggt atggtgaggt tttattgtct ttttgtcatg aaataatgtt tttaattaag
    10261 agtggggttt gatggacatt tcaagctggt tcgaaagtat tcatgtgttt ttaatactcc
    10321 tgaacggcgt tttttttaga ttggctccat tatttttctt tcttccattt ttaaataacg
    10381 gtataatttc tccatctatt agaatacctg tgatttttct tgttgcatca ggattaatta
    10441 cttctggtaa ggtagacata ggttcttctg tttttgaaca tgtttatttc cttatgttca
    10501 aggaaataat tgttggcctc cttctctctt tttgcttgtc tcttcccttt tggatatttc
    10561 atgctgttgg tagcattatt gacaaccagc gtggggcaac gcttagtagt tcaattgatc
    10621 ctgccaatgg tgttgatacg tctgagttgg caaaattttt caatcttttt tctgcagttg
    10681 tatttctata cagtggtggt atggtcttta ttttagaatc catacaattg tcttataata
    10741 tatgcccgtt attttctcaa tgttctttcc gtgtctcaaa tatcttaaca tttctgactt
    10801 tattggcaag tcaggctgtt attttagcca gtcctgttat gatagtattg ttactatcag
    10861 aagtattact tggtgtatta tcgagatttg ctccgcagat gaatgctttt tccgtatcat
    10921 taactattaa aagtttactt gcaatattta ttattttcat ctgttcttct actatttact
    10981 tttctaaagt tcaatttttc ctcggtgaac ataaattttt cacaaatcta tttgttagat
    11041 aaaatattat ggcaaataaa acagaaaagc cgacacctaa aaaactaaag gatgccgcaa
    11101 aaaaaggaca gtcatttaaa tttaaggatt taacgactgt tgttattatt ctggtaggga
    11161 catttactat aatatcattc ttttccttaa gtgatgtaat gctcttatac agatatgtaa
    11221 taattaatga cttcgaaatt aatgagggta aatacttttt tgcagtggtt attgtctttt
    11281 ttaagataat tggcttccca ctttttttct gtgttctttc ggctgtgttg ccaacattgg
    11341 ttcaaacaaa gtttgttctt gcgactaaag ctatcaagat tgatttttca gtattaaacc
    11401 ctgttaaagg gttaaaaaaa atatttagta taaagacaat aaaagaattt ttcaaaagca
    11461 ttctgcttct tattattcta gcattaacaa cctatttctt ttggattaat gaccgaaaaa
    11521 taattttttc tcaggtgttt tctagtgttg atggcttata tcttatttgg gggaggctgt
    11581 ttaaggatat aatattattt ttcttggcat tttctattct tgttattatt cttgactttg
    11641 tgattgagtt cattttatac atgaaagata tgatgatgga taaacaggag ataaaaagag
    11701 aatatataga gcaagaggga cactttgaga caaagtcgag aaggcgtgag ttgcatatcg
    11761 agattctttc agagcagact aaatctgata tacgtaattc aaaattagtg gtaatgaacc
    11821 cgactcatat tgcaattggt atttatttta atccagaaat agcgcctgca ccttttattt
    11881 ctctcattga aactaaccag tgtgccttgg ctgtcagaaa atatgcaaat gaagttggta
    11941 taccgactgt gcgtgatgtg aaattagcta gaaaactata taaaacacat acaaaatata
    12001 gttttgttga ttttgaacac ttggatgaag tcctacgtct tattgtttgg cttgagcagg
    12061 ttgaaaacac tcattaataa gtaaaggaga tgtgttatat gggagttaat ttctgtaata
    12121 aaataggtat tgatcagtct gaatttgaaa tagaatcttc catcattaac tccattgcta
    12181 atgaggtatt gaacccaata tctttcctta gcaataagga tataataaat gttttactca
    12241 gaaagatttc atctgaatgt gacctggtta gaaaagacat ttatcgttgt gctctggagt
    12301 tggtcgttga aaaaacacct gatgatttat aatcattcat aataactctt ttgctgatat
    12361 aaatattttt gttgaagata tttatgtagt cacaggatca attgtagaaa attgttatgt
    12421 ttttgttagg aactgcacta aataatatat gataagacag caaaaacgac tgaccataat
    12481 ccttctttta ttaggagttg ataagagaga ctattcatcc tgtaatgtta aaactctgtt
    12541 atatagtatt cgcgattatg caaagtctgt aaatgatcat gagatactaa ccgagagtaa
    12601 tcgtttatta tctcattgca tttcagatag taacggagct ttctttaaaa gtagtaaata
    12661 cgttcccctt aagtacttac gtaaaaggag aatagcacga aagataccaa atgattagca
    12721 ctttgctagt aagcttgctg gtaaactata ttgaccgttg ggtttgtttt gagactgtaa
    12781 tttaaattgt gtaattgcct gtttttgata tgtttactcc aacaacggag acaggtaaat
    12841 tatggacgaa aagaagctta aagcccttgc ggctgaactg gctaaaggcc tcaaaactga
    12901 ggccgacctc aatcagtttt cccgtatgct gacgaaatat cccggctctt aaaacgatag
    12961 tggctcaggc tttcattttt cagcgttccc cagaag
//
LOCUS       DAJTNP010000097        12809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTNP010000097 DAJTNP010000000
VERSION     DAJTNP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1176)
                     /locus_tag="OO160_003188"
     CDS             complement(<1..1176)
                     /locus_tag="OO160_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5525204.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="OO160_003189"
     tRNA            complement(1375..1459)
                     /locus_tag="OO160_003189"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="OO160_003190"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="OO160_003190"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5525205.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4305
                     /locus_tag="OO160_003191"
     CDS             2803..4305
                     /locus_tag="OO160_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5525206.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO160_003192"
     CDS             complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO160_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5525207.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO160_003193"
     CDS             complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO160_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5525208.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6873..8384
                     /gene="pepA"
                     /locus_tag="OO160_003194"
     CDS             6873..8384
                     /gene="pepA"
                     /locus_tag="OO160_003194"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5525209.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8518..8961
                     /gene="holC"
                     /locus_tag="OO160_003195"
     CDS             8518..8961
                     /gene="holC"
                     /locus_tag="OO160_003195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5525210.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8961..11816
                     /gene="valS"
                     /locus_tag="OO160_003196"
     CDS             8961..11816
                     /gene="valS"
                     /locus_tag="OO160_003196"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5525211.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11870..>12809)
                     /locus_tag="OO160_003197"
     CDS             complement(11870..>12809)
                     /locus_tag="OO160_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5525212.1"
                     /translation="AILMTVSADMPLVGCVLTLLLLVLLIFMAAKGLRYQALMTSLNG
                     VRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGI
                     VSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGY
                     IIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNH
                     FMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTY
                     LLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3241 a         3328 c         3373 g         2867 t
ORIGIN      
        1 catcaaccta cgctccccaa gatgttccgc cttatggatg taagctgctc gcacagagct
       61 acgctcctgg tgactcatct gccgctctac cgcatccctc gaccataatc ccgactcaat
      121 caacgaacta caagccatcg tcctgaagcc atgcccacaa acctcgacct tcgtgtcata
      181 ccccattacg cgcaaagcct tgttcaccgt attctcactc atcggcttac gtggatcgtg
      241 atcgcccaca aaaatcagct ctcgatttcc gctcatgctt ttgatctttt ccaaaatact
      301 tagggcctgc cgcgacaaag gtacaaggtg tggtgtccgc atcttcgagc cacgctgaga
      361 atgcttgact ccttccagtt gctcacgctc acctgggatt gtccacatag ccgtttcaaa
      421 atctatttct gaccagcggg caaaacgcag ctcgctcgaa cggatgaaaa ccaataaagt
      481 gagttcaacc gccagtcgag ttaacggtct gccagaatag tgatctatgc gatgaagtaa
      541 ttcaggtata cggttcagtt caagagctgc acggtgctgt cttttcgccg tagcaaccgc
      601 acctgcaatc tcttgcgcgg ggttgtagtc gattaagccg ctctgaacgg caaagcgcat
      661 aatcgcggta gtacgctgct gtaaacgggc ggcaacttcg agccgcccgg atgactcgac
      721 tgctttaatg ggtacaagaa gatcccgcgt cttcagttcc gcaatgttcc gcttaccgat
      781 agcagtaaag agattatcct ccagactttt caaaacacga gcgctatgcg atgccgacca
      841 cttctgattg ctggcgtgcc agtctctggc gactacttca aacgttatcg cctcttgttc
      901 ctgttctacc ttaacagctt tcttgttttc actgggatcg acaccattag ctaaaagctt
      961 acgagcctca tcccggcgtg ccctggcatc cgccaacgac acttcggggt atttccccaa
     1021 tgccagcatc ttctctttgc cgccaaagcg ataacgtagc cgccagtatt tggagccgtt
     1081 agggtgaacc agcaaaacca tgccttcacc gtcggtcagc ttataggctt ttgcttcagg
     1141 cttagccgaa cgaaccttca catcactcag agccatgatg agtatccttt caagggttct
     1201 gtgtgggtac aaacattatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt
     1261 tgatgtagat tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga
     1321 tactggattt taggcacaaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggca ccccgccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggcccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 agtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagatcaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgttcgg ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg gggagaaaaa gataagtcag acgtgatctg
     4321 tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga
     4381 ctttgtcaac aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta
     4441 acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg atgatcggcg
     4501 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4561 cgaagctgat acctgtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4621 taaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4681 acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     4741 tgtgcaaacc actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     4801 gcgtgaggtc ggttttccag gtgccgctca ccgtctgcga accggtaatc tgtttcggat
     4861 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     4921 tcgcggtagc ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     4981 tgatgccacc taacacctcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5041 ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5101 ggtagttacg cgccatctgt tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5161 gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata cgggtaaaac
     5221 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5281 ccccaagaag agccgccatc gggaagaaga tctgcacatc tttcggcacg ctcagcaagg
     5341 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     5401 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5461 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5521 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5581 cgccaaagcc agataaatca ggttaacggt ccacatccac agtgtcgggt ccagcttacc
     5641 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     5701 ggctggcagc atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac
     5761 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     5821 agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg
     5881 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     5941 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6001 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6061 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6121 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6181 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6241 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6301 tgccgctttc accagaaccg ctttgctcaa gccgcaggca tgcattaccg taatttcact
     6361 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6421 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6481 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6541 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6601 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6661 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     6721 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     6781 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     6841 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     6901 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     6961 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgccggg
     7021 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7081 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7141 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcgatg gaagcggtct
     7201 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7261 tcgagacagc aaaagagacg ctctacagtt tcgatcagtt gaaaacgaat aagagcgaac
     7321 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7381 gtgagcgcgc gatccagcac ggtcaggcga ttgccgccgg gataaaagca gcgaaagatc
     7441 tcggtaatat gccgccgaat atctgtaacg ccgcttatct cgcctctcag gcgcgccagc
     7501 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     7561 agctgggaat gcactcttat ctggcggtcg gtcagggttc acaaaacgaa tcgctgatgt
     7621 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7681 aaggtttaac cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga
     7741 tgaagtacga tatgtgcggc gcggcggcag tttacggcgt gatgcgtatg gtcgcggagc
     7801 tgcaactgcc gattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc
     7861 gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     7921 acaccgatgc cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg
     7981 agccggaagc agtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8041 atcatattac tggtctgatg acgaaccata atccgctggc ccatgaactg attgccgcgt
     8101 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8161 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8221 caggttgctt cctgtcacgc tttacccgta agtataactg ggcgcacctg gatattgcag
     8281 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8341 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct
     8401 caccctaacc ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct
     8461 catctttcag ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg
     8521 aaaaacgcaa cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt
     8581 gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtactcatc
     8641 gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca
     8701 gaaagctttg ttccgcataa tttagcggga gaaggaccgc gcggcagtgc gccggtggag
     8761 atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca
     8821 agctttgcag attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat
     8881 tctctgaaac aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg
     8941 aatacggcaa cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc
     9001 tttacgagca ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa
     9061 gtttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct
     9121 tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc
     9181 tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
     9241 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa
     9301 tctgggaatg gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca
     9361 actccgtcga ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga
     9421 aagaagtttt cgttcgtctg tataaagaag acctgattta ccgtggcaag cgcctggtaa
     9481 actgggaccc gaaactgcgt accgctattt ctgacctgga agtggaaaac cgcgaatcga
     9541 aaggttcgat gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta
     9601 aagattatct ggtggttgcg accactcgcc cggaaaccct gctgggcgat accggtgtag
     9661 ctgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc
     9721 tggttaaccg tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg
     9781 gctgcgtgaa gatcacaccg gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg
     9841 ccctgccgat gatcaacatc ctgacctttg acggtgatat ccgtgaaagc gcccaggtgt
     9901 tcgataccaa aggtaacgaa tctgacgttt attccagcga gatcccggca gagttccaga
     9961 aactcgagcg ttttgctgca cgtaaagcag tcgttgctgc ggttgatgca ctcggcctgc
    10021 tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtggtta
    10081 tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcag
    10141 ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact
    10201 tctcctggat gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc
    10261 gtatcccggc atggtatgac gaagcgggta atgtttatgt tggccgcaac gaagaagaag
    10321 tgcgcaagga aaataacctc ggtgccaacg ttgccctgcg tcaggacgaa gacgttctcg
    10381 acacctggtt ctcttctgca ctgtggacct tctctaccct tggctggccg gaaaataccg
    10441 acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcatcttct
    10501 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac
    10561 cgcaggtgcc gttccacact gtttacatga ccggtctgat tcgtgacgac gaaggtcaga
    10621 agatgtccaa atccaagggt aacgttattg acccgctgga tatggttgac ggtatttcgc
    10681 tgccagaact gctggaaaaa cgcaccggca atatgatgca gccgcagctg gcggacaaaa
    10741 tccgtaagcg caccgagaaa cagttcccga acggcattga gccgcacggc accgatgccc
    10801 tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc
    10861 gtctggaagg ctaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
    10921 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgcta tcgctggcgg
    10981 accgctggat tctggcggag ttcaaccaga ctatcaaagc gtaccgcgaa gcgctggaca
    11041 gcttccgctt cgatatcgcc gcaggcattt tgtatgagtt cacctggaac cagttctgcg
    11101 actggtatct ggagctgacc aagccggtaa tgaacggtgg aacagaagcc gaactgcgcg
    11161 gtactcgcca tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca
    11221 ttccgttcat caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg
    11281 acaccatcat gctgcagccg ttcccgcagt atgatgcatc tcaggttgat gaagccgcac
    11341 tggccgacac cgagtggctg aaacaggcga tcgttgctgt acgtaacatc cgtgcagaaa
    11401 tgaacatcgc gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag
    11461 aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct ggcgcgtctg gaaagcatca
    11521 ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac caagatcatc gacggcgcag
    11581 agctgctgat cccgatggct ggcctcatca acaaagaaga tgagctggcg cgcctggcga
    11641 aagaagtggc gaagatcgaa ggtgaaatca gccgcatcga gaacaagctg gcaaatgaag
    11701 gctttgtcgc ccgcgcaccg gaagcggtta tcgcgaaaga gcgtgagaag ctggaaggtt
    11761 atgcagaagc gaaagccaag ctgattgaac agcaggctgt tatcgccgca ctgtaatcaa
    11821 cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    11881 gtaaagaagg cataactccc cgagaaatac tggctaagaa gcctttatct ggttgttctt
    11941 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    12001 agtcaatcat ccatattttc agcagtgggt aagccagacc acccgtaatc ccggatatca
    12061 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    12121 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    12181 aactggtgct aacagcaatc ccaaaataat aaattaactg cgcgattatc attttacgtt
    12241 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcataccca gaactatcat
    12301 actcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    12361 ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc accttgaaac
    12421 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    12481 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    12541 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    12601 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat
    12661 taaaactaaa tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg
    12721 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagcggca
    12781 tatctgctga cactgtcatt aagattgcg
//
LOCUS       DAJTNP010000098        12800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTNP010000098 DAJTNP010000000
VERSION     DAJTNP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..105)
                     /locus_tag="OO160_003198"
     CDS             complement(<1..105)
                     /locus_tag="OO160_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5525213.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPK"
     gene            complement(311..1030)
                     /locus_tag="OO160_003199"
                     /pseudo
     CDS             complement(311..1030)
                     /locus_tag="OO160_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1046..1378)
                     /gene="cusF"
                     /locus_tag="OO160_003200"
     CDS             complement(1046..1378)
                     /gene="cusF"
                     /locus_tag="OO160_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5525214.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1402..2784)
                     /gene="cusC"
                     /locus_tag="OO160_003201"
     CDS             complement(1402..2784)
                     /gene="cusC"
                     /locus_tag="OO160_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5525215.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2941..3624
                     /gene="cusR"
                     /locus_tag="OO160_003202"
     CDS             2941..3624
                     /gene="cusR"
                     /locus_tag="OO160_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5525216.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3614..5056
                     /gene="cusS"
                     /locus_tag="OO160_003203"
     CDS             3614..5056
                     /gene="cusS"
                     /locus_tag="OO160_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5525217.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5206..7443
                     /gene="nrfB"
                     /locus_tag="OO160_003204"
     CDS             5206..7443
                     /gene="nrfB"
                     /locus_tag="OO160_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5525218.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7430..10398
                     /gene="nfrA"
                     /locus_tag="OO160_003205"
                     /pseudo
     CDS             7430..10398
                     /gene="nfrA"
                     /locus_tag="OO160_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10399..11289
                     /locus_tag="OO160_003206"
     CDS             10399..11289
                     /locus_tag="OO160_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5525219.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11472..12233
                     /gene="envY"
                     /locus_tag="OO160_003207"
     CDS             11472..12233
                     /gene="envY"
                     /locus_tag="OO160_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5525220.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12279..12355)
                     /locus_tag="OO160_003208"
     tRNA            complement(12279..12355)
                     /locus_tag="OO160_003208"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12319..12321),aa:Arg,seq:tct)
     gene            12598..>12800
                     /locus_tag="OO160_003209"
     CDS             12598..>12800
                     /locus_tag="OO160_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5525221.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3132 a         3320 c         3247 g         3101 t
ORIGIN      
        1 cttcggtgaa attcgtacgc tgaggccatc agcatcagcc aattcttgtg ggccagaata
       61 tggtttacca taaatagtac gcagttttgt gtcgcttata gccataaata atatttggta
      121 cgctcagaaa taatggtttg gtacacatct ggtacacaat atcacatgga caacagcgca
      181 cagtaaacaa ctatatttga cgttcgtaga catactcagg tgaaaaaaag gttgtttttc
      241 gagttataac agacagttaa ttaactacag tggacaatgc tagacaatga cagacacaga
      301 taagcaacct accttcctct ttcgcgacca cgttatcttt ggcgatattc attcccgcgc
      361 gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt gagcgtaaag cgagtctgga
      421 ttttttgcgt ggcgatcagg tggcgaatat ccgcctccgg cattcccgcc agtcgcagcc
      481 gctcaagaat gccttcagtc tgggtcgccg taccgccggt ttcgcgcaac agtaaatact
      541 cactctgcgc ttccacccag tcgggaatgg tcaggtcgag aagcggtgtg cccttttgta
      601 ctttatcgcc cacggtaagc ggatacacct tgtcgataaa cccggcagcg cgagcctgca
      661 caatggcata ctgatactcg ttgtaactga cattcgtcgg gaaactctgg gcaaaagtca
      721 gcggcccgcg cgtgacggta gccgttttca cccccagatt ctgcgtctga gtcgggtcaa
      781 tgcgcacacc agacgcagaa ctctcttcat cggcatattt cggcaccaga tccatatcca
      841 taaacggcga tttacctggt ttatcgaacc gcgtattggg atacatcggg tcgtaccaga
      901 ataagatttt acgttctgcg gtcggcattt tttctgcgtg cggttccgcc tggtcaaacc
      961 aggtaaaacc tgccgctgaa ataataccgc ccgcgatcat gctgccgata ataagcgcga
     1021 tttttttcat ctcattaaac ctgggttact ggctgacttt aatatcctgt aataaagaaa
     1081 ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc ggttttaatt tcactcattt
     1141 tcgtctgcgg ggtgatggta aagcgcatgg tcatctccgg ccagttgacg gcagcaatcg
     1201 gatcgtgatg gatggtgatt tttttgcttt ccagatcaac accttttacc acaccggtgg
     1261 cactaatgac ctgtggttgt gcttcgctca tggtttcatg atgatgttcg ttagcctggg
     1321 cattaaagcc aataacggta aacagactga acattgcgac ttgcagtgct tttttcattt
     1381 attctctcct ggagttaaaa attattgctg ccaaccgccc cctaaggcgg tatacaaata
     1441 aatttcgtta acctgacggg cataattcag gtcaagtaaa gtttgtcggg ttgcaaataa
     1501 agaacgctcg gcatccagca cttccagata acttaccgcg ccgtgctgat ataatgcccg
     1561 cgcccgttgc agggtaattt gcagcgacgc cagataacgc tgctgggcgc tgatttgatc
     1621 gtccaggctt tgacgtaatg ccagcgcatc tgccacctct ttaaaggcgt tctggatttt
     1681 ctgttcataa ttcaccaccg actgctgctg gcgaatttcg gcgatatcca gattggcctg
     1741 gttgcgtccg gcattaaaaa tggggatctc aattttagga ataaaattcc acatcccgct
     1801 gctagcgtta aataatgacg acagatcgct gctggcggtt gatattccgc tggtcaggct
     1861 gatagacggg aaaaatgccg cacgcgcagc accaatatta gcattagccg ccattaacgc
     1921 gtgttcagct tccataatat ccgggcgctg caataagatt tgcgacgaca agcccgccgg
     1981 taatttaacg ctttgcaggc tgccgctgtt tactgtctgc gcttgcggta gcttgccgta
     2041 gcttcccaat aacagttgca atgcattatt cgcctgcgcc agttccccct gacgtttagc
     2101 gatgtcactg cgggtacttt ctatcacccc gcgagcctgt tccagcgcca gaacattgct
     2161 gctaccggtc aacagttgtt tttcgacaaa cgcatatgac tgctgataat tacgcagcgt
     2221 ttcttcggct atttgcaatt gcgcatacgc cagctgctga ttgaaatagc tttgcgcgac
     2281 attagaaacc agcaaaatat gcaccgcgcg ctgggcttcc tcagtggcta aataattttg
     2341 gcgctcggct tcgctcatgt tctttaagcg accgaaaaaa tcgagatcaa agctggcgtt
     2401 aaggccagtc gagaactccc gcgtcgtggc tgaatcgcct ttaagattgc cgctccagct
     2461 gccgctgccc tcgccattga gctgtgggta gcggtcggca tcggtcagac gatattgcgc
     2521 ccgtgcctcc tgcactttca gcgtcgccat gcgcaaatcc cggttattca ccagcgcctc
     2581 gctaatcagc gtcttcacct gattatcaac aaaaaaggtg cgccagccca cgttctggta
     2641 gttatccgct gcgttaacca ggccgttctg gctgagtgag aactgctgcg gtacgggcat
     2701 taccggacgc tgataatccg gtgccagtga acaaccagtt agcgcaaggg ccacacaaaa
     2761 tggcagaagt ttacaaggag acataggctc ataatttctg gtgattttat gtcgccaact
     2821 ttactcgcca ggctctgatt ttccggtgac aggaaaatga caaaattgtc attttgccaa
     2881 taagcgattg ccatctgatc ccgctactct agaattgccc gggcaacatg cggaggaaat
     2941 atgaaactgt tgattgtcga agatgaaaag aaaaccggag aatacttgac caaagggtta
     3001 accgaagccg gttttgtggt cgatttggcc gacaacgggc tgaatggcta ccatctggcg
     3061 atgaccggtg attatgacct gataatcctc gatattatgc tgccagacgt gaacggctgg
     3121 gatatcgtgc gcatgttgcg ctccgccaat aaagggatgc cgattctgtt gcttaccgcg
     3181 cttggcacca ttgaacatcg cgtcaagggg ctggagttgg gggcagatga ctacctggtg
     3241 aaaccgttcg cttttgctga actgctggcg cgggtgcgca ctctcctgcg gcgcggggcg
     3301 gcggtgatta tcgaaagtca gtttcaggtt gccgatctga tggtcgatct cgtcagccgc
     3361 aaagtcaccc gcagcggcac gcgcatcact ctgaccagta aagaatttac tctgctggag
     3421 ttttttctcc gccatcaggg cgaagtgctg ccccgctcgc ttatcgcctc gcaggtatgg
     3481 gacatgaatt tcgacagcga cactaacgcc attgatgtgg cggtgaagct gctacgcggc
     3541 aaaatcgaca acgactttga gccgaagctg attcaaaccg tgcgcggcgt gggttacatg
     3601 cttgaggtgc cggatggtca gtaagccatt tcagcgcccg ttttcgctgg caacccgcct
     3661 gacctttttt atcagccagg ccaccatcgc ggcgtttttc gcctttgcat ggatcataat
     3721 ccactcagta aaagtgcatt ttgccgagca ggacattaat gatttaaaag agattagcgc
     3781 cacccttgaa cgggtactaa atcaccctga cgaaacgcaa gcccgacgct taatgacgct
     3841 ggaagatatc gtcagtggtt attccaacgt gttgatttcc ctggcagata gccacggcaa
     3901 aacggtgtat cactcccccg gtgcgcctga tatccgcgag tttgcacgtg atgccatacc
     3961 cgataaagac gcgcggggtg gcgaggtgta tctcctttcc ggcccgacga ttatgatgcc
     4021 aggccacggt cacgggcata tggaacacag caactggcgg atgattaact tgccggttgg
     4081 cccgttggtg gacggcaaac cgatttatac gctctacatc gcgctgtcga tcgattttca
     4141 tcttcattac ataaatgatt tgatgaataa acttattatg accgcatcgg taatcagcat
     4201 cctgatcgtc tttatcgtgc tgttggcggt acataaaggt cacgcgccga tccgcagcgt
     4261 cagccgtcaa atccagaata tcacctcgaa agatctcgac gttcgccttg acccgcagac
     4321 cgtgccgatt gagctggaac agctggtact gtcgttcaac catatgatcg agcgtattga
     4381 ggatgtcttt actcgccagt ccaatttctc agcggatatc gcccacgaaa ttcgcacacc
     4441 aattactaat ctcattacgc aaacggaaat cgccctcagc cagtcgcgca gccagaagga
     4501 gctggaagat gtgctctact ctaatctcga agagctgacg cgaatggcga aaatggtcag
     4561 cgatatgctg tttctcgctc aggccgataa caaccagcta atccccgaaa agaaaatgct
     4621 caacctggcg gatgaagtcg gcaaagtgtt cgattttttc gaggcgctag cggaagatcg
     4681 cggcgtggag ttgcggtttg ttggcgacaa acgtcaggtt gcgggcgatc cgttgatgct
     4741 gcgtcgggcg ctaagcaacc tgctttctaa tgcccttcgt tatacgccaa ccagagagac
     4801 aattgtagta cgctgtcaga cggtcgatca ccaggtgcaa gtttccgtcg aaaaccccgg
     4861 tacgcccatt gcgcccgagc acttaccgcg attgtttgac cgtttctatc gcgttgaccc
     4921 ctcccgccag cgaaaaggtg aaggtagcgg tattgggctg gcgatagtga aatcgattgt
     4981 tgtcgcgcat aaaggcacgg ttgcggtaac gtcagatgcg cgggggacaa ggtttgttat
     5041 cacattaccc gcttaattat tcagcaaacg gcaactttta taaccagtgt aaaaataacg
     5101 tgccgcaata gtcatacata ttaatggtaa aaagctgtca caattcataa aaaaccttaa
     5161 tatacgccac cctaaacata accagcgtta atgtaaggtt tttgtgtgga ctggcttctt
     5221 gatgtttttg ctacctggct ctacggctta aaagtaatcg cgataacgtt agcggtcatc
     5281 atgttcatca gcgggctgga cgattttttt attgatgtcg tctactgggt acgccgcatt
     5341 aaacgcaagt tgagtgttta tcgccgctac ccgcgaatga gttaccgcga actgtataaa
     5401 ccagatgaaa aaccgttagc gattatggtt ccggcgtgga atgaaacggg cgtcatcggc
     5461 aatatggccg agctggcggc gaccacgctc gactacgaaa actatcatat ctttgttggc
     5521 acctacccca acgaccccga tactcaacgt gatgttgacg aagtgtgcgc tcgctttccg
     5581 aacgtgcata aggtagtctg cgcgcgtcct ggtcccacca gcaaagccga ctgcctgaac
     5641 aacgtgctgg acgccatcac ccagtttgaa cgtagcgcca atttcgcttt tgctggtttt
     5701 attctgcatg acgccgaaga tgtgatttca ccgatggaat tgcgtctgtt caactatctg
     5761 gtcgagcgta aagatctgat tcagatccca gtgtatccgt ttgaacgcga atggacgcac
     5821 ttcaccagca tgacttacat tgatgagttt tcagaactgc atggcaaaga tgttccggtg
     5881 cgtgaagccc tcgccggaca agtgcccagc gcaggcgtcg gcacctgttt cagccgccgc
     5941 gccgtgaccg cactgttagc tgacggtaac ggtattgctt tcgacgtgca gagtcttact
     6001 gaagattacg acattggctt ccgcctgaaa gaaaaaggta tgacggaaat ttttgtccgt
     6061 tttccggtgg tggacgaagc caaagaacgc gagcagcgta aatttttaca gcacgcacgg
     6121 acgtcaaaca tgatctgcgt gcgcgaatat ttccccgata ccttttcgac tgcggttcga
     6181 caaaaatctc gctggatcat cggcattgtt ttccagggct ttaaaaccca taaatggacc
     6241 tccagcctga cgctgaacta ctttctctgg cgcgaccgca aaggggcaat cagtaacttt
     6301 gtcagcttcc tcgcgatgct ggtgatgatc cagcttttgc tgttgctggc gtatgaaagt
     6361 ttgtggcccg atgcctggca tttcctttct atttttagcg gcagcgcatg gttaatgacc
     6421 ctgctgtggc taaactttgg cttgatggtt aaccgcatcg tgcagcgagt gattttcgtc
     6481 actggctact acggcctcac gcagggcctg ctttccgtcc tgcgtctttt ctggggcaac
     6541 ctgattaact ttatggctaa ctggcgcgtg ctgaaacagg ttcttcagca cggcgatccg
     6601 cgtcgcgtcg cgtgggataa aacaacgcat gacttcccca gcgtcactgg cgatacccgc
     6661 tcgttgcgcc cgttaggtca aattctgctg gaaaatcagg tcatcactga agaacaactc
     6721 gatacagcac tgcgtaatcg cgtcgaaggt ctacgcctgg gcggttcaat gctgatgcag
     6781 gggctgatta gcgccgagca gctggcacag gcgctggcag agcaaaacgg cgtggcgtgg
     6841 gaatccatcg atgcctggca gatcccgtcc tcgctgattg ccgaaatgcc agcctccgta
     6901 gcgctgcatt atgcagtact gccgctgcgt ctggaaaatg atgagttaat tgtcggcagt
     6961 gaagatggta ttgacccggt ttcgctggcg gccctgacgc gtaaagtcgg acgcaaagtg
     7021 cgttacgtca ttgttctgcg gggacaaatt gtcacagggt tacgccactg gtatgcacgc
     7081 cgacgcggtc acgatccgcg ggcaatgttg tacaatgcgg ttcagcatca gtggctcacg
     7141 gaacagcaga ccggtgaaat ctggcggcaa tatgtgccgc atcagttcct gttcgccgaa
     7201 atactgacca cgctcggtca tattaatcgt tcagcaatta acgtgttgtt attgcgccat
     7261 gaacgcagtt ctctgccgct cggcaagttt ttggtcaccg aaggcgttat cagccaggaa
     7321 acgttggatc gcgtcctgac aattcaacgc gaattacaag tttcgatgca atcactatta
     7381 ctcaaagcag gtttaaacac agaacaggtt gcgcaactgg agtccgaaaa tgaaggagaa
     7441 taaccttaat cgcgtcatcg gatggtctgg tttactgctg acgtctttat tgagtaccag
     7501 cgcactcgca gacaatatcg gcaccagcgc agaagagctg gggctgagcg attatcgcca
     7561 ttttgttatt tatccccgtc tcgataaggc gctgaaggca cagaaaaata acgacgaagc
     7621 aaccgccatc cgcgaatttg aatatataca ccaacaggtg ccggataata ttccgctgac
     7681 tttatacctt gcggaagcct atcgtcattt tggtcatgat gaccgggcgc ggctgttgct
     7741 tgaggatcaa ctgaaacgtc acccaggaga tgcccgactt gagcgcagtc tggcggctat
     7801 tccagttgaa gtgaaaagcg ttacgactgt tgaagaactg cttgcccagc aaaaagcggg
     7861 cgacgctgcg ccgaccctgc gttgtcgcag tgaagtcggg cagaatgccc tgcggctggc
     7921 acaattacct gtcgccagag cgcaactgaa cgatgcgacg tttgctgcat cgccggaagg
     7981 aaaaacgctg cgaaccgatc tgctgcaacg ggcaatctac ctgaaacaat gatcccaggc
     8041 agatacgcta tacaatgaag cacgccagca gaacacatta agcgcggcag aacgccgtca
     8101 gtggtttgac gtgcttcttg ccgggcagct ggacgatcgg atcctggcac tgcaatcaca
     8161 ggggatcttc accgatcctc agtcatatat tacttacgcg accgcgctgg cttatcgtgg
     8221 cgaaaaagca cgcctccagc attatctcat tgaaaataag ccgctgttta ccacgggcgc
     8281 acaagagaaa agttggctct atctgttatc taaatacagc gccaaccccg ttcaggcatt
     8341 ggcgaattat acggtgcagt ttgccgataa ccgccagtat gttgttggcg cgacgctacc
     8401 ggtgctgtta aaagaaggtc agtacgacgc agcgcaaaaa ctgctcgcca ccctccccgc
     8461 caatgaaatg cttgaggagc gttatgctgt cagcgtggcg acccgtaaca aggctgaatc
     8521 tctgcgtctg gcacgattgc tgtatcagca agaaccgaca aatcttcccc gcctggatca
     8581 actgacctgg caactgatgc agaacgagca gtcacgcgaa gctgccgatt tattgctgca
     8641 acgctatcct ttccagggcg atgcgcgtgt cagccagact ttaatggcgc gactggcgtc
     8701 tctgctggaa agtcatcctt acctagcaac gccggcgaag gtggcgattt tatcgaaacc
     8761 cttaccgctg gcggagcaac gtcagtggca aagtcagttg ccgggtattg cagataattg
     8821 cccggcaata gttcgcttgc tgggcgatat gtcgccttcc tacgatgctg ccgcttggaa
     8881 ccgtctggca aagtgttatc gggacacgct acccggtgtg gcgttgtatg catggcttca
     8941 ggccgaacaa cgacaaccga gcgcctggca acatcgtgcg gtagcctatc aggcgtatca
     9001 ggttgaggac tacgccaccg cactggcggc ctggcagaaa atcagtcttc acgacatgag
     9061 caatgatgat ctgctcgccg ccgccaacac cgctcaggca gcgggaaatg gcgcagctcg
     9121 cgatcgctgg cttcaacagg cagaacaacg tgagctggga aacaatgctc aatactggtg
     9181 gctgcatgcg cgacgttata tcccagctca gccagaactc gcactgaacg atctcacgcg
     9241 ctcaatcaat attgcccctt ctgccaactc ttacgttgcg cgggcgacaa tttatcgcca
     9301 acgtcataat gttccggccg cggtgagtga tttgcgcgcc gcgctggaac tggaaccaaa
     9361 taatagcaac acccaggcag cgcttggtta cgccttgtgg gatagcggtg atatcgcaca
     9421 gtcgcgggaa atgctcgaac aggcgcataa agggcttccg gacgatccgg cactgatccg
     9481 acaactggcc tacgtgaacc agcgtctgga tgacatgcct gcgacgcagc actacgcccg
     9541 gctcgtgatt gatgacattg ataatcaggc ataaccccac tgaccccaga gcaaaatcag
     9601 caacgcttca atttccgccg tttgcatgag gaggtcggtc gccgctggac gttcagtttc
     9661 gattcttcca tcggcttgcg ttccggcgca atgagtaccg ctaacaataa tgtcggcggt
     9721 gcagcgcctg ggaaaagcta tcgtagctac ggacaactgg aagccgagta ccgcatcgga
     9781 cgcaatatgc tgctggaagg cgacctgctc tcagtttata gccgcgtctt tgccgatacc
     9841 ggagaaaacg gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg tctgcgctgg
     9901 aagccgctgc gcgatcagat ctttttcctc gccgtcgaac agcagttgcc gctgaacggg
     9961 caaaatggcg catccgatac catgctgcgc gccagcgcct cattctttaa tggcggcaaa
    10021 tacagcgacg aatggcaccc gaacggttca ggctggtttg cccaaaacct gtacctcgat
    10081 gcggcgcaat atatccgcca ggatattcag gcgtggacgg cagattatcg cgtcagctgg
    10141 catcagaagg tagctaacgg acagactatt gagccttacg ctcacgttca ggacaacggc
    10201 tatcgtgata aaggcactca gggcgcgcag cttggcggtg tcggggtccg ctggaatatt
    10261 tggaccggcg agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg cgtcgagtat
    10321 caacatacct ttaaggcgat taatcaacgt aacggagagc gcaacaacgc gtttctcacc
    10381 attggagtgc actggtaaat gcgtaagttc attttcgtat tgctgacact gcttttggtc
    10441 agcccttttt cctttgcgat gaaaggtatt atctggcaac cacaaaaccg agatagtcag
    10501 gttaccgata cccagtggca ggggctgatg agtcagttac gttttcaagg cttcgatacc
    10561 ctggttttgc aatggacccg ttacggcgat gcatttaccc agccagaaca gcgcacgtta
    10621 ttgtttaagc gtgccgcagc tgcgcaacag actggcctga agctgattgt cgggctgaac
    10681 gccgatccgg aattttttat gcaccagaaa cagtcgtctg cagcgctgga aagctatctt
    10741 aatcgcctgc tggctgccga tctccagcaa gccagattat ggagcgccgc gcctggcata
    10801 acgccggatg gctggtacat cagcgcggaa attgacgacc tgaactggcg cagcgaagcc
    10861 gcccgtcaac ctttgctaac atggttaaac aacgcgcagc ggctgattag cgatgtttca
    10921 gcaaaaccgg tttatatcag tagttttttc gccggaaaca tgtcgcccga tggctatcgc
    10981 caactgctgg aacaagttaa agcaaccgga gttaatgtct gggtacagga tggcagcggc
    11041 gtggataaac ttaccgctga acagcgtgaa cgttatctac aggccagcgc cgattgccaa
    11101 agctccgccc cagcaagcgg cattgtttat gaactgtttg tcgccggcaa aggcaaaacc
    11161 tttacagcga aaccgaaacc ggacgcagaa attgcctcgc tgttagcgaa acgttcctct
    11221 tgcggtaaag acatgctcta tttctctctg cgctatttgc ccgtcgcgca cggcattctc
    11281 gagtattaaa tcccctccag gtaagtcggg tacgacctgg cttacctctt tcgctctcgt
    11341 cagaattcca tcaagattaa ttgctaaaaa gcatgctaaa gaaagaagat cgttatcggc
    11401 gaacgggccg atcactcatg caggaagcat agatcctttg agcggagttt ctgatatctg
    11461 aggagtgcga aatgcaattg agcagcagtg aaccttgcgt ggtgatcctg accgaaaaag
    11521 aggtagaggt aagcgtcaat aaccatgcta cgtttaccct tccgaaaaac tacctggccg
    11581 ccttcgcgtg caacaataac gtcattgaac tctcaacgtt aaatcacgta ttaatcaccc
    11641 acatcaaccg taacatcatc aacgattatc tgttgttttt aaataagaaa ttaacctgtg
    11701 taaagccctg gtcgcggcta gcaaccccgg ttatcgcttg tcatagccgt acaccggaag
    11761 tgttccggct agccaccaac cacagcaagc agcaatccag caaaccctgc gaagcggagt
    11821 tgacgcgcgc attacttttt accgtattgt ctaactttct tgagcaatcg cggtttattg
    11881 ccctactgat gtatatctta cgcagcagcg tccgcgacag cgtctgccgc attattcaaa
    11941 gcgatattca gcattactgg aatctgcgaa ttgtcgccag ttcgctatgt ttaagcccca
    12001 gcctgctcaa aaagaaatta aaaaacgaaa ataccagcta tagccagatt gtcacagagt
    12061 gtcgtatgcg ttacgccgta cagatgttat tgatggataa caaaaatatc actcaggtgg
    12121 cgcaattatg tggctatagc agcacgtcgt actttatctc tgtttttaag gcgttttaca
    12181 gcctgacacc gttgaattat ctcgccaaac agcgacaaaa agtgatgtgg tgaagggcaa
    12241 agcggaaacg gataagacgg gcataaatga agaagaaatg gcgcgccctg caggattcga
    12301 acctgcggcc cacgacttag aaggtcgttg ctctatccaa ctgagctaag ggcgcgttga
    12361 taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac ccttgctgag tcaatggctt
    12421 ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt ttctgattta tttcgctata
    12481 gcggcaaaca aacgcacacc gctgcgcgtc tgaatcaaga aaacccgtat tttcatgtat
    12541 caaagtacaa tttaccgccc taacggaaaa ttgtccgctc ctatgagact ggtaactatg
    12601 aaaccaacgt cggtgatcat tatggatact catcctatca tcagaatgtc tattgaagtt
    12661 ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa cggatgatta ccgcataacc
    12721 atcgattatc tccgaacccg tcctgttgat ttaatcatta tggatataga cttgcccgga
    12781 acagacggtt ttaccttcct
//
LOCUS       DAJTNP010000099        12703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTNP010000099 DAJTNP010000000
VERSION     DAJTNP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO160_003210"
     CDS             complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO160_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5525222.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1033..2124
                     /gene="rutA"
                     /locus_tag="OO160_003211"
     CDS             1033..2124
                     /gene="rutA"
                     /locus_tag="OO160_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5525223.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2124..2816
                     /gene="rutB"
                     /locus_tag="OO160_003212"
     CDS             2124..2816
                     /gene="rutB"
                     /locus_tag="OO160_003212"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5525224.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2828..3214
                     /gene="rutC"
                     /locus_tag="OO160_003213"
     CDS             2828..3214
                     /gene="rutC"
                     /locus_tag="OO160_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5525225.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3222..4022
                     /gene="rutD"
                     /locus_tag="OO160_003214"
     CDS             3222..4022
                     /gene="rutD"
                     /locus_tag="OO160_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5525226.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4032..4622
                     /locus_tag="OO160_003215"
     CDS             4032..4622
                     /locus_tag="OO160_003215"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5525227.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4633..5127
                     /gene="rutF"
                     /locus_tag="OO160_003216"
     CDS             4633..5127
                     /gene="rutF"
                     /locus_tag="OO160_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5525228.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5148..6476
                     /gene="rutG"
                     /locus_tag="OO160_003217"
     CDS             5148..6476
                     /gene="rutG"
                     /locus_tag="OO160_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5525229.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6558..6665)
                     /locus_tag="OO160_003218"
     CDS             complement(6558..6665)
                     /locus_tag="OO160_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525230.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7038..7634
                     /gene="wrbA"
                     /locus_tag="OO160_003219"
     CDS             7038..7634
                     /gene="wrbA"
                     /locus_tag="OO160_003219"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5525231.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7655..7882
                     /locus_tag="OO160_003220"
     CDS             7655..7882
                     /locus_tag="OO160_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5525232.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO160_003221"
     CDS             complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO160_003221"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5525233.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9454..10710)
                     /locus_tag="OO160_003222"
     CDS             complement(9454..10710)
                     /locus_tag="OO160_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5525234.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10971..11891
                     /gene="cbpA"
                     /locus_tag="OO160_003223"
     CDS             10971..11891
                     /gene="cbpA"
                     /locus_tag="OO160_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5525235.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11891..12196
                     /gene="cbpM"
                     /locus_tag="OO160_003224"
     CDS             11891..12196
                     /gene="cbpM"
                     /locus_tag="OO160_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5525236.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12289..>12703)
                     /gene="torD"
                     /locus_tag="OO160_003225"
     CDS             complement(12289..>12703)
                     /gene="torD"
                     /locus_tag="OO160_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5525237.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3034 a         3242 c         3201 g         3226 t
ORIGIN      
        1 tgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
       61 cggttaaaca ggtagccgga ggatgttaca acctcctccg tcatctttaa cgtggtcgaa
      121 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
      181 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
      241 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
      301 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
      361 catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
      421 gataatcgcg tgagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcgggg
      481 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
      541 cggcaatata cagcgcctct tttgacggga agtaatacag cagattggtt tttgaaacac
      601 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
      661 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
      721 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggt aacaggatag
      781 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacggatg catgttttat
      841 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata acacaatgct
      901 aacacattaa taatattcac tttttaaaaa ctggcatccg ctttgcgaac aagccaatac
      961 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
     1021 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
     1081 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgcgcaaaaa
     1141 gcggagcatt accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
     1201 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg tctggcagcc
     1261 gtgacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
     1321 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
     1381 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
     1441 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
     1501 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
     1561 caaccgagta tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
     1621 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac acccacggct
     1681 ttcgccccga ccgctgcgcg gatgaaacag gccacagagc aaaccggacg cgacgttggc
     1741 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccactcg cgccaaatgg
     1801 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
     1861 aaagataccc gctccggtac tgacaccaac gttcgtcaga tggccgatcc cacttcggcg
     1921 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
     1981 gaagtcgcaa gcgtgcctgg tgcagaaggc gtgctgttga ccttcgatga ttttctgtcg
     2041 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctctct
     2101 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
     2161 tcgatccgca gcaaagtgcg cagatcgtgg tggatatgca aaacgcttat gccacgccag
     2221 gcggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
     2281 ttcaaaccgc tgtgaccgca gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg
     2341 gctgggatga acagtatgtc gaggctggcg gccccggctc accgaatttt cataaatcga
     2401 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
     2461 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
     2521 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttacgc agccgtggaa
     2581 tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
     2641 atggcttttt tctggagcat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
     2701 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
     2761 acgtcgaaac gttctgcgac gcgctttctc ccacgtcctt tgctcgtatc gcttaaggag
     2821 tttagcgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
     2881 cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg cttttgatca
     2941 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
     3001 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
     3061 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
     3121 gggtgataaa ccggcgcgat tctacattca gtgcggactg gtaaaacctg acgcgctggt
     3181 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcat
     3241 ctccccctta tgctgatgca cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
     3301 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
     3361 agcgcggcac cggcaataat cccgacacac tggcagaaga ttacagtatc gcccagatgg
     3421 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
     3481 atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga ttatcccgcg tcggtaacta
     3541 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
     3601 ttcgcgaacg attactgtat agcggcggcg cgcaagcatg ggtggaagcg cagccgttgt
     3661 tcctctatcc cgccgactgg atggcggccc gcgcacctcg cctggaggca gaagacgcgc
     3721 tggcactggc gcattttcag ggcaaaaata atttactacg tcgacttaac gccctcaaac
     3781 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
     3841 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
     3901 gccagaaaat ggtgatgccc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
     3961 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
     4021 aaggaattgc tatgaacgaa gccgttagcc caggtgcgct tagcaccctg ttcaccgatg
     4081 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta caggagcttt
     4141 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
     4201 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
     4261 aaaccctgac cgcgcccgtc accgctatcg tcgcctggga cagtgaattt tatgaacggt
     4321 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
     4381 ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc ttatctgatc gtcgcttgcc
     4441 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaacac gttgacgacg
     4501 ccttttttgc gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
     4561 gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
     4621 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
     4681 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
     4741 agcgccgtct gcagcgtgac cgacacgccg cccacattac tggtgtgcct gaatcgtggg
     4801 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
     4861 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
     4921 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
     4981 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
     5041 atcgaagcga ttcatcgtca caccacaccc tacgggctgg tgtggttcga tcgcagttat
     5101 cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
     5161 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
     5221 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
     5281 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
     5341 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
     5401 gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
     5461 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
     5521 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
     5581 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
     5641 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
     5701 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
     5761 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
     5821 ttactctcgt cagctatgct gcctggttcg gtctgccgca tttttccact cctggtttta
     5881 atggccaagc gatgatgttg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
     5941 gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcgag
     6001 cattcgtcgg tgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtca
     6061 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
     6121 ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc gccgaaattt ggcgcgctga
     6181 tccataccat tccggccgcg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
     6241 ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga tttaagccag aacgctaatt
     6301 tgattatggt cgcagtaacc ttcgtgctgg gcgcgggtga ttttgctctc acgcttggcg
     6361 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttat
     6421 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
     6481 aaaaccgccg ggttaccggc ggtgatgact ttttgacgag cttaagaaaa aaatgcattc
     6541 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
     6601 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
     6661 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
     6721 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc ggacagaccc
     6781 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct ccaataatta
     6841 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
     6901 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
     6961 ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
     7021 acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg tacggacata
     7081 ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa agtggatggc gcagaagttg
     7141 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     7201 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     7261 gtacacctac ccgctttggc aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     7321 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     7381 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     7441 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     7501 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggctca cgccagccaa
     7561 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     7621 aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7681 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7741 cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt tgggaagaag gtagtgatga
     7801 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7861 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt ctcgcggggg agttttctgt
     7921 tatttcaccg cctcattcaa cacgctatca aacttatcca tcgggcagaa accgttggca
     7981 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     8041 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     8101 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     8161 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggctatg
     8221 ttggagtcgt gcccaactaa caccgtgatt ttcggagcac tggcgcgatc ggtgaccagg
     8281 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     8341 aacagactgt cctggtaacc gtttttcagc ttcgacaata ccttccactg ctggtcagat
     8401 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     8461 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cacctggttc ttgttgatac
     8521 ttggcgctaa aggtattttt gccatccacc agcgaacact gttgtttctc tttacaggca
     8581 ggggaatctt tatagttaac gattttttcc agtagctggt agctgtcggt aagctggagt
     8641 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8701 gtgatcatcg ggttaaaggt tgggtccata gtgcccattt tttcctggtg atgcacagga
     8761 atatcacacc cagggaatgc accagtaata aagaactggg cggtagcgac ggtacgttgc
     8821 agactattgg cataggcata aacggtgtcc ggcggcgggc attcccccga tttcactatc
     8881 ccctgctctg ccagccattc acgcatgtaa tggcccatat acacttcgag cacgccacct
     8941 ttggtggtga gttgcccacc ggggacgtcc cattcgggcc atttattcgg cgtcgactgc
     9001 tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatggcggct catcatgagc
     9061 acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga agcgagtaaa
     9121 actatccctg ccacagttgc ggcgattagc gttttgttca ttcctggcac ctcttttgtt
     9181 atcaatcatt aaagagtgtg acagaaatat gacttttttc cgggaacagg ttcgcaaaat
     9241 ggcttgttga acgcctgttt ttctatatac atcttcaaaa acaggcaagg tttgacgcaa
     9301 actatgcctg attagcccct gcctcgcgta cgccgctgat aattgcacct gtggctaaac
     9361 agaatatctg cggcgttacc aatacagaga caatatttac caataaaaaa gtgtgagact
     9421 aataacaatg gtttttagca tatgcctttg ccattaagtt gcataacgat ttaaatagtc
     9481 aagttgcgca ttcatttcct tattttttaa cttataagcc tgagaaaaaa gttcaggagg
     9541 gaggtaaaca tcataatgat tgccagtgtt tgttaaatat ccatactcaa gatcttcatc
     9601 tgttgaaaag gattcatttc ccaaggagat tgatgccaca ttactattaa caataaataa
     9661 tgagcaaatg ctatctgaaa gaacagcaag aaaattcttc accgctttaa tcctatccga
     9721 cgtgtcggta tctaaagcaa gcaaattctt gttatcatta aatataaact cgggtaaatg
     9781 cagtatgcat tttttgtcat tcaagcaact atcaatgaat tgttttttaa aaaacagaaa
     9841 gctccatgac ataaaactac ccacaccctg aattttgttg tcagattgca gatcaacgcc
     9901 aaataatgtc tttacatctt ccctgagatt atagaatgcc gtatcaacaa gagcctcgat
     9961 cgtttgttta tcaacaattt taataaactc ttcgtatact tgcttttgcg tcttatggct
    10021 gcccagcgcc catgaatact catgttcttt taaaaccagt atggctctta acagacaata
    10081 tccatcgcca tctactggag cgtattttaa tccatatgtt ttattaattt cactctgaat
    10141 aaaagatatg ccagacaaat caataccagt atctaaatca gatattcccg gcatatttat
    10201 ctgttctggc tggatactca tattttcatt atctaaacaa tactcagaaa aaacatcatc
    10261 acacgcatta atgttttcaa tatattccat aaagccatac caataataac ctttaaaatc
    10321 tgtgatagac tctgaacctc tgcccggcaa ggacactcta acaatatcaa catcttgaac
    10381 atattgtata gtatatgtaa tacctcctga aacccctgaa taaatttgtg tatcattagt
    10441 acgcaatgtt ctcataaata atgatagatc attatcattc tgcatttcct caaattcttt
    10501 ttctagtgat tcacattcca gacatggagt atcctcttca tttactttac gtctgagttc
    10561 attgtacaac cgcataaaac acaccagtgt gttttcaaaa agcgtgattg aatatgtttg
    10621 atttttttcg tactcatttt ttatatcgtt ccaggcctgt ttcaaataat tatttgcagt
    10681 gcaactaatt ccatgtatat tactacccat atatagcgtc tataaaattt aataaataat
    10741 gacgccctag ttaaacttaa agtgcctggt tcaactatca aaaatcgctc accctttttc
    10801 acctgtttaa aatatgttca gcaacccatc ttgatggcga cctcctctcc gcgatgattt
    10861 caataacata ttctgtgttg gcatatgaaa ttttgaggat taccctacac ttataggagt
    10921 taccttacag gggttccttc aatttgtgtt gatttacgcg agataacgct atggaattaa
    10981 aggattatta cgccatcatg ggcgtgaaac cgacggacga tctcaagaca atcaagaccg
    11041 cctatcgtcg actggcccgt aaataccatc ctgatgtcag caaagaaccg gatgccgaag
    11101 cccgcttcaa agaggtcgct gaagcctggg aagtgttaag tgatgaacaa cgtcgcgctg
    11161 agtatgatca gatgtggcaa catcgcaacg atccgcaatt taaccgtcag ttccatcatg
    11221 gcgacggtca gagttttaat gccgaagatt ttgacgatat cttctcgtca attttcggtc
    11281 agcatgcccg ccagagccgt caacgccccg ccgcacgtgg ccacgatatt gaaatcgaag
    11341 tggcggtatt cctcgaagaa acgcttactg agcataagcg taccatcagc tataacctgc
    11401 cggtttataa cgcctttggc atgatcgaac aggaaatccc gaaaacgctg aatgtgaaga
    11461 tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg acgccgggcg
    11521 aaaacggcgg tccaaatggc gatttgtggc tggtgattca tattgcgcca catccgctgt
    11581 tcgatattgt cggccaggat ctggaaattg tggtgccggt tagcccgtgg gaagcggcgc
    11641 tgggtactaa agtcaccgtt ccaacactga aagaaagcat tttgctgact atcccgccag
    11701 gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc aaaaaacaga
    11761 ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat gaaaacactg
    11821 ccgcgctgtg gcagcaactg gcagacgccc agtcgtcttt tgatccacgt aaagattggg
    11881 ggaaagcata atggctaatg ttacggtgac ttttactatt accgaatttt gcctgcatac
    11941 cggcatctct gaagaggagt tgaatgaaat tgtcggtttg ggggtggttg aaccgcgtga
    12001 gatccaggaa acaacctggg tatttgacga ccatgccgcc attgtggtgc aacgcgcggt
    12061 acgcctgcgt catgaactgg ctctggactg gccggggatc gcggtggcgc tgacgttaat
    12121 ggatgatatt gcgcacctga agcaggaaaa ccgcctgcta cgccagcggc tttcccggtt
    12181 tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac gcatccggca actgcgccat
    12241 aatgcctgat gcgacgctaa acgcgtcttt tcaggcgcat aaacgacgtt atctgttttg
    12301 gtggtcgcac tccactaaca ccagcaataa ctggcttagt gccgcgtaaa aaccaaagct
    12361 gtcatactgt cgacaacgcg cagcaaactc tggtaaccat tgccgcagcg ccgtcagtgt
    12421 tttttgccgc aaactgtcga ttcttcgcgc aggaacggtc ccctctccca gcgaaaaatg
    12481 cagataactc aacaactcga gatagatcgc cagatgatct gccggttcgt tgaaattgcc
    12541 actggtttcc atccctgcct caactaacaa gcgtttaatc tcttgctcgt cctgtttgta
    12601 ggccgatgca tacggcagcg ccgcttgttt gtcggtcatc agaaacaggc cgcaaaagtc
    12661 agcggccagt tccagacggg catcgtcacg tactgtcagc gtg
//
LOCUS       DAJTNP010000100        12629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTNP010000100 DAJTNP010000000
VERSION     DAJTNP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            758..841
                     /locus_tag="OO160_003226"
     CDS             758..841
                     /locus_tag="OO160_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5525238.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO160_003227"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO160_003227"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5525239.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO160_003228"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO160_003228"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5525240.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO160_003229"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO160_003229"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5525241.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO160_003230"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO160_003230"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5525242.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO160_003231"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO160_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO160_003232"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO160_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5525243.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO160_003233"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO160_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5525244.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO160_003234"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO160_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5525245.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="OO160_003235"
     CDS             10966..12165
                     /locus_tag="OO160_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5525246.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3108 a         3169 c         3356 g         2996 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    12301 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    12361 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    12421 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    12481 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    12541 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    12601 tgccatgaga gtcgtgatat tattattca
//
LOCUS       DAJTNP010000101        12463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTNP010000101 DAJTNP010000000
VERSION     DAJTNP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO160_003236"
     CDS             complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO160_003236"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5525247.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1796..3214)
                     /locus_tag="OO160_003237"
     CDS             complement(1796..3214)
                     /locus_tag="OO160_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5525248.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3368..3712)
                     /locus_tag="OO160_003238"
     CDS             complement(3368..3712)
                     /locus_tag="OO160_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5525249.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO160_003239"
     CDS             complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO160_003239"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5525250.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4702..5637)
                     /locus_tag="OO160_003240"
     CDS             complement(4702..5637)
                     /locus_tag="OO160_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5525251.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENTLGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5717..6832
                     /gene="yneK"
                     /locus_tag="OO160_003241"
     CDS             5717..6832
                     /gene="yneK"
                     /locus_tag="OO160_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5525252.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6982..8172
                     /gene="ydeA"
                     /locus_tag="OO160_003242"
     CDS             6982..8172
                     /gene="ydeA"
                     /locus_tag="OO160_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5525253.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO160_003243"
     CDS             complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO160_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5525254.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9074..9508
                     /gene="marR"
                     /locus_tag="OO160_003244"
     CDS             9074..9508
                     /gene="marR"
                     /locus_tag="OO160_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5525255.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9528..9911
                     /gene="marA"
                     /locus_tag="OO160_003245"
     CDS             9528..9911
                     /gene="marA"
                     /locus_tag="OO160_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5525256.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9943..10161
                     /gene="marB"
                     /locus_tag="OO160_003246"
     CDS             9943..10161
                     /gene="marB"
                     /locus_tag="OO160_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5525257.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO160_003247"
     CDS             complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO160_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5525258.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11355..>12463
                     /gene="ydeE"
                     /locus_tag="OO160_003248"
     CDS             11355..>12463
                     /gene="ydeE"
                     /locus_tag="OO160_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5525259.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPA"
BASE COUNT         3274 a         3213 c         2854 g         3122 t
ORIGIN      
        1 caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata ggatgttgta
       61 actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta
      121 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
      181 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
      241 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
      301 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
      361 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
      421 gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
      481 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
      541 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
      601 ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
      661 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
      721 tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata
      781 tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa
      841 gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac
      901 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
      961 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
     1021 cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc
     1081 tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc
     1141 cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
     1201 ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc
     1261 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
     1321 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt
     1381 gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag
     1441 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
     1501 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
     1561 tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt ggtgcgacag
     1621 gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca
     1681 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
     1741 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
     1801 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
     1861 acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg
     1921 atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg
     1981 tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag
     2041 gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct
     2101 tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt
     2161 catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca
     2221 gatgttgtgt ctgtttcagc gatggacttt tcagcacctc ataaagaccg gaccgtgagt
     2281 aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcaaattga
     2341 tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac
     2401 gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat
     2461 ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg
     2521 gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc
     2581 cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc atcatctgtt
     2641 ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca
     2701 acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac
     2761 tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac
     2821 agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc agacgcttat
     2881 cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca
     2941 gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga
     3001 tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc
     3061 cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat
     3121 agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga
     3181 acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg
     3241 tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt cgctaacagc
     3301 ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct
     3361 gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt
     3421 cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg
     3481 tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt
     3541 cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga
     3601 tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag
     3661 agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac
     3721 cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc
     3781 tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac
     3841 aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc
     3901 gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt
     3961 caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg
     4021 ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt
     4081 tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg
     4141 cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc
     4201 gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa
     4261 catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa taccctgttc
     4321 catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg
     4381 ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat
     4441 agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt
     4501 acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc
     4561 gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt
     4621 atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat
     4681 atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga tattacagaa
     4741 ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa
     4801 ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg
     4861 tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa
     4921 ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgcagc
     4981 aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac
     5041 cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta acaaacgcgc
     5101 accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc
     5161 cattggtccg agagtgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc
     5221 tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc
     5281 cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag
     5341 ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca
     5401 ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag caatgcgcga
     5461 gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg
     5521 cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca tcacattcgg
     5581 cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac
     5641 ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg atgacttatg
     5701 actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat ctcttcctga
     5761 tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg attttattga
     5821 tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca ccaactcagc
     5881 cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga cttcttttcg
     5941 gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc ctgtcaagaa
     6001 tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc ctatttattt
     6061 agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc caaagcctgt
     6121 cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt ttatgaatga
     6181 ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag cgcatacacc
     6241 acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac gccagtttga
     6301 tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc gattatggaa
     6361 taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca ccgccattct
     6421 cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg attcattagg
     6481 ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt tagcagaaca
     6541 agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat tgcactatca
     6601 ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag catcattaag
     6661 tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg taaaaaatga
     6721 aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct cccctgtcag
     6781 accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt aaaccgtgat
     6841 gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa cgcgatccag
     6901 atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat cactttttcg
     6961 caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt ggcgtggcta
     7021 cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt
     7081 ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt cggcatcatg
     7141 ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt aatgaccagt
     7201 caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc cagccacgta
     7261 ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt
     7321 gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc tccggccggg
     7381 aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat ggtcttaggt
     7441 ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt cttcgcgatt
     7501 ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt actgcctagt
     7561 gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc attgatgagc
     7621 atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag ctatatcgag
     7681 ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt actgttatta
     7741 ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa tcaatatgcg
     7801 tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact gctgctacct
     7861 gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat cgcgatgatg
     7921 atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc taccgacgtc
     7981 gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc gttggtaggt
     8041 aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct
     8101 tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac actcgaagaa
     8161 cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt taatgatttc
     8221 caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc ccatcaggcg
     8281 ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg agctgcgtag
     8341 gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac
     8401 ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa tcatcgctat
     8461 ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat tggcactggg
     8521 ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg ctttctgttg
     8581 cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc
     8641 gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg ccaccatcat
     8701 gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg cactgttcat
     8761 gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta acggcagcaa
     8821 caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt gttatcctgt
     8881 gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt cactctttag
     8941 ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg gcatcggtca
     9001 attcattcat ttgacttata cttgcctggg caatattacc ccctacaact aattacttgc
     9061 caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc cattgggtcg
     9121 cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc tgtctccgct
     9181 ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac
     9241 tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc gtatgctgga
     9301 tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata agcgcggcgt
     9361 actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc aattagttgg
     9421 ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg caacacttga
     9481 gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg tccagacgca
     9541 atactgacgc tattaccatt catagcattt tggactggat cgaggacaac ctggaatcgc
     9601 cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac ctgcaacgga
     9661 tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt aagatgacgg
     9721 aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca gaacgatatg
     9781 gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat gttccgccgc
     9841 ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca ttaaatcatt
     9901 acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc actttcatcc
     9961 gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca aaccacgcag
    10021 ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga acaaccaccg
    10081 tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg cgtgccctat
    10141 tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt attaacttcc
    10201 cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    10261 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    10321 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    10381 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    10441 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    10501 atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc tccagattac
    10561 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    10621 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    10681 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    10741 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    10801 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    10861 gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag
    10921 tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    10981 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    11041 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    11101 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    11161 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    11221 tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct ggtagagcgt
    11281 cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt attttagaga
    11341 tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    11401 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    11461 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    11521 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    11581 ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    11641 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    11701 ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    11761 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    11821 aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    11881 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    11941 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    12001 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    12061 cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg ctgatgacag
    12121 caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc aacaacctgc
    12181 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    12241 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    12301 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    12361 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    12421 tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgc
//
LOCUS       DAJTNP010000102        12449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTNP010000102 DAJTNP010000000
VERSION     DAJTNP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..1030
                     /locus_tag="OO160_003249"
     CDS             <1..1030
                     /locus_tag="OO160_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5525260.1"
                     /translation="NLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVW
                     FDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYD
                     AEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVI
                     GGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPH
                     DGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFN
                     TCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAP
                     IRPIARRT"
     gene            1031..3196
                     /locus_tag="OO160_003250"
     CDS             1031..3196
                     /locus_tag="OO160_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5525261.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3210..4121
                     /locus_tag="OO160_003251"
     CDS             3210..4121
                     /locus_tag="OO160_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5525262.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4121..5416
                     /locus_tag="OO160_003252"
     CDS             4121..5416
                     /locus_tag="OO160_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5525263.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5461..5691
                     /locus_tag="OO160_003253"
     CDS             5461..5691
                     /locus_tag="OO160_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525264.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5669..6169
                     /locus_tag="OO160_003254"
     CDS             5669..6169
                     /locus_tag="OO160_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5525265.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6169..7587
                     /locus_tag="OO160_003255"
     CDS             6169..7587
                     /locus_tag="OO160_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5525266.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7587..8435
                     /locus_tag="OO160_003256"
     CDS             7587..8435
                     /locus_tag="OO160_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525267.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8435..8890
                     /locus_tag="OO160_003257"
     CDS             8435..8890
                     /locus_tag="OO160_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5525268.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8893..9585
                     /locus_tag="OO160_003258"
     CDS             8893..9585
                     /locus_tag="OO160_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5525269.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9595..10926
                     /locus_tag="OO160_003259"
     CDS             9595..10926
                     /locus_tag="OO160_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5525270.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10927..>12449
                     /locus_tag="OO160_003260"
     CDS             10927..>12449
                     /locus_tag="OO160_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5525271.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALG"
BASE COUNT         3341 a         3089 c         3283 g         2736 t
ORIGIN      
        1 gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg aagatggcat
       61 ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg gtgacacaat
      121 ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag gtcttacccg
      181 taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg ggatgtctga
      241 cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca acatgaccat
      301 ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat catatcccga
      361 gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc gaatcttcca
      421 gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact tctacgtaat
      481 tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt ggtgggataa
      541 ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa cagccgttca
      601 ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc ctcatgatgg
      661 aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag acgctggatt
      721 tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg agccaggcat
      781 cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata cctgtgagcc
      841 attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg tcaagcttaa
      901 cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg caaaaatgat
      961 gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc ccatcgcacg
     1021 gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt ttgacgcaga
     1081 ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt ttagccgagt
     1141 gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg gacaattcga
     1201 tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc ctgtcgacgt
     1261 tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga tggggatgta
     1321 tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg ttcgtgagca
     1381 gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca acgatccaac
     1441 aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac acgtcatatg
     1501 ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg tgattaacgc
     1561 cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc cggataccct
     1621 gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga atgatgtcgt
     1681 ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca tctaccgcga
     1741 cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg tcatcgacga
     1801 cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc ggcgtgtgta
     1861 taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag ccggagagca
     1921 tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg agtgctacga
     1981 gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta tgtcgttcaa
     2041 cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg agcaaatcga
     2101 aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc agaacaggac
     2161 tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa accctgaagt
     2221 gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag aagtggctag
     2281 tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg tcaatcaact
     2341 gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta ccgcaatgcg
     2401 acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc ctcgtcatgt
     2461 aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc aagttgtcga
     2521 ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg agtgctatac
     2581 agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga ttcaggagtt
     2641 gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc aatacttcac
     2701 gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc aactggtgat
     2761 gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc aggcacaaca
     2821 acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc tgcaaggtca
     2881 ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag ctgccaaggt
     2941 tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca atatggacct
     3001 cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc agcaacagcg
     3061 cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gaagcagacc agactcattc
     3121 acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg gcggagtagc
     3181 cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt caggcttctg
     3241 aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta gttgtcgata
     3301 atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa gacgatgaga
     3361 aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc gaacgcaaac
     3421 gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg ccggagcacc
     3481 tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt tccgaagatg
     3541 cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag caggcaaaca
     3601 gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag cagggtcgca
     3661 aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt aagcactacg
     3721 aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca ttcatgcaac
     3781 tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa tccgccgctc
     3841 tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg atggacgggc
     3901 aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag cctcgagcca
     3961 agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct gttgctgcaa
     4021 atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat gtagagacgt
     4081 accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa atgaaggtca
     4141 actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga cgccaatggc
     4201 gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa gcaacaccgt
     4261 gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa ctggcaacgc
     4321 aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata acgatttctt
     4381 cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca tccaggcatc
     4441 cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa ctgaaatggg
     4501 ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc ctggctggga
     4561 ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg acatgggcat
     4621 cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg tagatggtga
     4681 catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc gtcagattgc
     4741 tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt caaccgcgac
     4801 tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc ttgataccga
     4861 tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct ccaccaccgg
     4921 atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc agatggcgaa
     4981 gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg gtactcacat
     5041 cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag aagagaaggc
     5101 ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc tgaacgtggc
     5161 aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt ctcagccgat
     5221 cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca ttccaggtat
     5281 tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg gtaagtgccg
     5341 tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg ttggtctgcc
     5401 taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct ggagacaagc
     5461 atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt tggctacgac
     5521 tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg gttttcacat
     5581 cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca gcgtaaaaag
     5641 cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg atttagttct
     5701 tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg tcgaaccgca
     5761 gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc ttggcggtga
     5821 tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg ctccggatcc
     5881 gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca accttgcctg
     5941 ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca ctgccagata
     6001 cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag ctaaatgtaa
     6061 gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga agtggaatgg
     6121 ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat gcctattcag
     6181 caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta tatcgactat
     6241 ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg atatcttcgc
     6301 tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg cgtcgagtac
     6361 aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa aggagaaagt
     6421 gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg gacatcacag
     6481 gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt caaaaccgtc
     6541 tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt tcgcgacatt
     6601 acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg gtttatcact
     6661 gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc cgagtctcag
     6721 cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt tggttcatcg
     6781 actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt gtatgttgca
     6841 caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac gccattcgct
     6901 gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta tatcatcgac
     6961 tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg ctcctacact
     7021 gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca tgagttgctg
     7081 attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc caatggccca
     7141 caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat cgacttcgtt
     7201 tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg gaaactgcaa
     7261 ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac tccactcttc
     7321 aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg cgttgcgcag
     7381 tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg gcgtgagcag
     7441 atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa gcgagtaggg
     7501 cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc tgtcactctg
     7561 tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg ccggtaatca
     7621 tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg cagtcgtatc
     7681 tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa gccaacgagg
     7741 ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg attctcgctg
     7801 accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag gagcgaatca
     7861 gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat gaaagcgata
     7921 tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac catgaaacgc
     7981 gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt tactctggtg
     8041 tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa acgctcacac
     8101 cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg gttgtgttca
     8161 acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc agcggaaccg
     8221 ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg gtcagcagtc
     8281 gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc gtggataaag
     8341 acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt gcggcgttta
     8401 ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
     8461 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
     8521 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
     8581 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
     8641 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
     8701 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
     8761 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
     8821 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
     8881 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg cgaggaacct
     8941 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
     9001 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     9061 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     9121 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     9181 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     9241 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     9301 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     9361 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     9421 cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag cggtggcacg
     9481 tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac gccttggggc
     9541 gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg aatcatggct
     9601 acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa ctcaaacgct
     9661 ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa tgatattgag
     9721 cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg tattgcgggt
     9781 gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc gtacgctaat
     9841 gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata tccagaccag
     9901 attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa ttctatcggc
     9961 accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca atcatggctg
    10021 caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt cgctcagatg
    10081 taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat gaacagtctc
    10141 gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt taccatgcgc
    10201 ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat gcgcggacaa
    10261 gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac aaaccgcatt
    10321 aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga cctgaagcgc
    10381 caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa gcagcaagcg
    10441 ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt cactctgaac
    10501 gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt aatccccaac
    10561 gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa atcacaagca
    10621 tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc cgagggcaaa
    10681 aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc ctttcgtcgc
    10741 aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt tggtaagtac
    10801 gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga ttacctgaag
    10861 cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc atctttgggg
    10921 cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta cggtgtgcca
    10981 gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc cgcagtaagc
    11041 tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga tatgggttat
    11101 accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg ctatctggca
    11161 aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca cgacggtccc
    11221 ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg ccgcggttac
    11281 gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac agtcgaacag
    11341 cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt tgcacaactg
    11401 gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc aaataatcca
    11461 tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt aagttctgtc
    11521 gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc aacacgggaa
    11581 gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag tgcagggcaa
    11641 attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt gcagggtggc
    11701 gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt ggatgatgtt
    11761 tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc aataggcggt
    11821 tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct cgctgacgcg
    11881 gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact tagcattgct
    11941 acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa tataattagt
    12001 ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc accattctcc
    12061 ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag agtagcacaa
    12121 ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac agtagcagca
    12181 caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc tcaggctgaa
    12241 gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca cttttccggg
    12301 aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc tgctctacag
    12361 gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat aattgatcgc
    12421 gtttccggtg caaaagatgc tcttggtat
//
LOCUS       DAJTNP010000103        12309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTNP010000103 DAJTNP010000000
VERSION     DAJTNP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..401
                     /locus_tag="OO160_003261"
                     /pseudo
     CDS             1..401
                     /locus_tag="OO160_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OO160_003262"
     CDS             484..741
                     /locus_tag="OO160_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525272.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO160_003263"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO160_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5525273.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO160_003264"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO160_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5525274.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO160_003265"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO160_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5525275.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO160_003266"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO160_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5525276.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO160_003267"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO160_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5525277.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO160_003268"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO160_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5525278.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO160_003269"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO160_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5525279.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OO160_003270"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OO160_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OO160_003271"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OO160_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OO160_003272"
     CDS             9762..10019
                     /locus_tag="OO160_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5525280.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(10339..10470)
                     /locus_tag="OO160_003273"
                     /pseudo
     CDS             complement(10339..10470)
                     /locus_tag="OO160_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10752..12110
                     /locus_tag="OO160_003274"
     CDS             10752..12110
                     /locus_tag="OO160_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5525281.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         2926 a         3233 c         3362 g         2788 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agcaattgaa ccgccccggg tttcctggag
    10321 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
    10381 tttttctgct tctgccggtg ggatatggcc ctgcctttcc agcaatcgtc gattgttata
    10441 ccagtccacc cacgtgagtg tggtcagctc atcatactga cacgtgcatt acccctaccg
    10501 cgataatgca accgtacaat atagggcgtt tcctaaacgc agccctcatc actccaatcc
    10561 aacaagacac agcatttttg atcacgaaga aagacaaata atttccgtaa atgtttccgt
    10621 tgttacaaat aacaacagtg ttttacattt ctttggcctc aggcccgcgc atgttgccat
    10681 tcgatgtcat tttcctgact cgtttcttct ttatggtgta gtgtgatctt cactttatag
    10741 atgctaacaa tatgaaaaga aaaattattc ccgtgctaat cggttgtacg ctctctttct
    10801 ctgccctggc ggcacaacct acggctgagc gctatgtcgt cagttttcca gaaggcacgc
    10861 acgtgaacta cgccggcgca tttgccagtg cattcccgaa cggccttccg gttgggatag
    10921 gttctggcct gctgttcacc ggtaaacagg gcgatgccct gacattcgca accattaccg
    10981 atcgcggtcc gaacgcggat tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta
    11041 cgccagattt cgcgccgctg ctgatgacga ttcgtgtaca aaacggcaaa gcggaggcca
    11101 tcgatccccg tcctctgcat gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg
    11161 acgtgattgg ctccaccaat gaagtggcct tcagcgacac gttacataga ctgaaggggg
    11221 ataatcgcgg gctggatacg gaagggatca ctccggatgg taaaggaggt tactggctgt
    11281 gcgatgagta tggcccgttc ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc
    11341 acggtccgca ggcggcggaa ggggaaaaag cgattgcggg cggtttgcca aatatcctca
    11401 aatggcgtca ggcgaaccgc ggctttgaag gcctcacccg tatgccggac gggcgtatta
    11461 ttgtcgccgt gcaaagtacg ctggatatcg acgctaagag caagaaaaag gcgctgttta
    11521 cgcgtctggt gagcttcgat ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg
    11581 acagcgcggc ctacagcaaa aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata
    11641 accagcacat cctgctgatc gaacagggaa gagataaaaa caacagaatg cgtaacctga
    11701 tttacgaggt cgatctcaat aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt
    11761 atccggagtt tgatgatgag aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa
    11821 cgcaggttgt tgatctgcgc tccttgggct ggcagcagga gaaggctgaa gggttggcgc
    11881 tgatcgacag caaaacgctg gcggtggcga acgataacga ctttggcgtg aaggtggcga
    11941 tgcaacatcc ggtcgagggc aagacattca aggattatcg ggtgaacgcg gaaggaaagc
    12001 tgacgcttga cgacaagcag gttgaaacga ccctcagagt gaagccactg gagaaaccgg
    12061 agtctgacag tgaactgtgg attgtgacgc tgccggaagc gttgaaataa gtttataagg
    12121 tacggacagc agtatcgctt agtttttcaa attcatgtat gtgaacaata ttggggttcc
    12181 ggcgtagacc cccaatgaaa gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc
    12241 atcccatggt ggcagccggt taactttcct gcgccaccag tggaaaaagg ccataagcag
    12301 tccgaacaa
//
LOCUS       DAJTNP010000104        12219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTNP010000104 DAJTNP010000000
VERSION     DAJTNP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..492)
                     /locus_tag="OO160_003275"
     CDS             complement(<1..492)
                     /locus_tag="OO160_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5525282.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO160_003276"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO160_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5525283.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO160_003277"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO160_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5525284.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO160_003278"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO160_003278"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5525285.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO160_003279"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO160_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5525286.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO160_003280"
     CDS             3646..4155
                     /locus_tag="OO160_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5525287.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO160_003281"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO160_003281"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5525288.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO160_003282"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO160_003282"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5525289.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO160_003283"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO160_003283"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5525290.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO160_003284"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO160_003284"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5525291.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO160_003285"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO160_003285"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5525292.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO160_003286"
     CDS             complement(8797..9690)
                     /locus_tag="OO160_003286"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5525293.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO160_003287"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO160_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO160_003288"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO160_003288"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5525294.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="OO160_003289"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="OO160_003289"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5525295.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJTNP010000105        12087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTNP010000105 DAJTNP010000000
VERSION     DAJTNP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..1260
                     /gene="maeA"
                     /locus_tag="OO160_003290"
     CDS             <1..1260
                     /gene="maeA"
                     /locus_tag="OO160_003290"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5525296.1"
                     /translation="IKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYT
                     LPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDF
                     AQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSA
                     GCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSD
                     WDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSR
                     VEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
                     RITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK
                     TSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1394..2404
                     /gene="adhP"
                     /locus_tag="OO160_003291"
     CDS             1394..2404
                     /gene="adhP"
                     /locus_tag="OO160_003291"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5525297.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2550..2834
                     /locus_tag="OO160_003292"
     CDS             2550..2834
                     /locus_tag="OO160_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5525298.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3062..3715)
                     /gene="fdnI"
                     /locus_tag="OO160_003293"
     CDS             complement(3062..3715)
                     /gene="fdnI"
                     /locus_tag="OO160_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5525299.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(3708..4592)
                     /gene="fdnH"
                     /locus_tag="OO160_003294"
     CDS             complement(3708..4592)
                     /gene="fdnH"
                     /locus_tag="OO160_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5525300.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4605..7652)
                     /gene="fdnG"
                     /locus_tag="OO160_003295"
     CDS             complement(4605..7652)
                     /gene="fdnG"
                     /locus_tag="OO160_003295"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7065..7067),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5525301.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            7884..8765
                     /gene="yddG"
                     /locus_tag="OO160_003296"
     CDS             7884..8765
                     /gene="yddG"
                     /locus_tag="OO160_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5525302.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9020..10120
                     /gene="ompC"
                     /locus_tag="OO160_003297"
     CDS             9020..10120
                     /gene="ompC"
                     /locus_tag="OO160_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5525303.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10284..10769
                     /locus_tag="OO160_003298"
     CDS             10284..10769
                     /locus_tag="OO160_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5525304.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            10879..11868
                     /locus_tag="OO160_003299"
     CDS             10879..11868
                     /locus_tag="OO160_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5525305.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3170 a         2986 c         2813 g         3118 t
ORIGIN      
        1 attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga ccagggcatc
       61 ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg cggcatcagc
      121 ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca gctgcttaac
      181 gatccgctgt atatgggctg gcgtaatccg cgtatcactg acgatgaata ctatgaattc
      241 gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt gcagtttgaa
      301 gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga aatttgttct
      361 tttaacgatg acattcaggg cactgcggcg gtaacagtcg gcacactgat cgcagccagc
      421 cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc aggttcagcg
      481 ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt aagcgaggaa
      541 gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga caagatgccg
      601 aacctgctgc ctttccagac caaactggtg cagaagcgcg aaaacctcag tgactgggat
      661 accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc agatattctg
      721 attggcgtct caggacagac cgggctgttt acggaagaga tcatccgtga gatgcataaa
      781 cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtctcgcgt ggaagccaca
      841 ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg cagcccgttt
      901 aatcctgtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa cgcctttatt
      961 ttcccgggca tcggcctggg tgttattgct tccggcgcgt cacgtatcac cgatgagatg
     1021 ctgatgtcgg caagtgaaac gcttgctcag tattcgccgc tggtcctgaa cggcgaaggt
     1081 ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc gtttgcggtt
     1141 ggcaaaatgg cgcagcagca aggcgtggcg gtcaaaacgt ctgctgaagc tttgcaacaa
     1201 gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac ctccatctaa
     1261 gcctgcaccc ggtagtgaag gctaccgggc tatttccctc tccctttttc agatctcatc
     1321 catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga acatcatccg
     1381 aaaaggagga actatgaaga ctgcagttgt tacgaaggat catcatgttg acgttacgga
     1441 taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt gttgtggtgt
     1501 atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaaccg gcgtaattct
     1561 gggccatgaa ggcatcggtg tggtggcaga agttggccca ggtgtcacct cattaaaacc
     1621 aggcgatcgt gccagcgtgg cgtggtttta cgaaggatgc ggtcattgcg aatactgtaa
     1681 cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg ttgatggcgg
     1741 gatggaggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag atggtctgga
     1801 ctcggcggcg cccagcagca ttacctgtgc gggggtcacc acctacaaag ccgttaaact
     1861 gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc tgggtaacct
     1921 cgccctgcaa tacgcaaaga atgtctttaa cgccaaagtg atcgccattg atgtcaatga
     1981 tgagcagtta aaactggcaa ccgaaatgga tgcagattta gcgattaact cacgcaccga
     2041 agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg tggtaacagc
     2101 agtagctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg gtcgtgttgt
     2161 ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgtcttg tgctggatgg
     2221 cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag ccttccagtt
     2281 tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg acatcaacac
     2341 catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg atttccgtca
     2401 ctaagaggtc tttgctgcga cccccatgtt cgggtcgcag catcgcacac tctccaacat
     2461 gaaatggcta aatggattat tgttgcgtag gaaggaacaa taatgatagt gttcccagag
     2521 aagtaacgct actgagagta aagacactaa tgaaaatggc caatcatccc cgcccggggg
     2581 acattattca ggaatcactg gacgaactta atgtcagcct gcgcgagttt gccagagcaa
     2641 tggaaattgc gccctcaacg gcaagccgat tgctgaccgg aaaagcagct ttgacgccag
     2701 aaatggcaat taaactttcc gtggtgatcg gcagttcgcc gcaaatgtgg ctgaatctgc
     2761 aaaacgcctg gagtctggca gaggcagaaa aaacggtggt tgtgtcgcga ttacgccgtc
     2821 tggttacgca ataaaaaatg gcaccctata aagcgccatt tgaaactgat gacaaacgca
     2881 acattgcctg atgcgctacg cttatcaggc ctacacgttc acttcaatct attgtatttg
     2941 aagggttttc atgccggata aggcattcac gacgcatccg ggatgaacaa agcccacgtt
     3001 gtctacaata tgaaaatggc gccttttaca gcgccatttt cagcttaaag ttcaaaagag
     3061 attatatccc ttcttcactc tcttttttcg cttctgcctt ctcgatttca cgataccagc
     3121 gcggatggtg tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc cctttaatcg
     3181 accctttcac ccagaatgcc atatacatat ggatcaggat ggcgtggatc aggatgatac
     3241 ccgcagccgc gtggatcagc aggctgtagc gaacaacctg catcgggaag tactgcgcaa
     3301 agtacggacg ccagataatc accccggtca ccagcagcac gaaaatcatg ctcatgatcg
     3361 accagaacat catcttttgc ccggcgttgt acttaccaac atccgccact ttgtgctcat
     3421 tgccttttaa tacttcgaca atgttcaaca gccacggaat atctttctta tccgggatgt
     3481 tgtgatgcac aaaacgcaca aacataaaca tcagtgcgac gaaaatcgca atgccgaaga
     3541 aggggtgcaa aatgcgtccc atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg
     3601 tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc accacggtcc
     3661 agtgacaggc gcgatcaata aatttggtgc gcacaatcat tttcgactta ctcatgatga
     3721 tcctcctcgt catcgtccac ttccttattc gggccaatac cgatgtagtg gaaaatcaac
     3781 ccggcaaaag tggcaataaa gccagccgct gccagcggtt tcaacgcgcc tttccacagg
     3841 cttaccgagg tgtcgatctt cggatctttc ggcagaccgt gatacagctc cggctgatcg
     3901 gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca
     3961 gcatgttcgt aaccacgcgc tttcagtttc gccacgcgct gttccgccag ctccagcatc
     4021 tccttcttgg tgccgaagtg gatagccccg gtcggacagg ttttcacaca agccggttcc
     4081 tggccgacgc tgacgcgatc gacgcagagc gtgcatttat atacccggtt atcctctttg
     4141 ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt aaccacagcc gatgcagttt
     4201 tccgactgga aatcgacaat cccgttagcg tactgaatga ttgcaccagc agacgggcac
     4261 gccttcaggc agcccggatc ttcacagtgc atacagccgt ctttacggat cagccactcc
     4321 agcttgccat tctgttcggt ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga
     4381 tcggcggggt tatcgtaaac cccgacgcag tgccccactt catcacggat gtcgttccac
     4441 tccgaacacg ccacctgaca ggctttacag ccgatacagg tggaaacgtc gataagtttc
     4501 gcgacttctg ctttgtaatc acgcacctga gaaggcggcg tgatggagtt agttgcggac
     4561 cttttgataa tatcctgcgt ttccatagcc atctgttcgc ccccttacgc cttctcgatg
     4621 ttgactaaga acgctttata ttccggcgtt tgcgagtttg catcaccgac attcggcgtc
     4681 agagtgttag cgatataacc tttacgcgcg acaccctcaa agccccagtg gattggaata
     4741 cccaccgttt caacctgctg accgtttaca ttcagcggtt tcagacggcg cgttaccact
     4801 gccaccgcgc ggataaagcc tcgcttgctg gagacagtga cacgatcgcc attattaatg
     4861 cctttcgccg ccgcctgcgt ttcgctgatt tccacaaact gttccggctg agcaattgcg
     4921 ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc
     4981 acatacggga actgctcttt tttacccatc cgcagcgcgt cttgttcata cagacgaaca
     5041 accgggttag acaccacgtt cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc
     5101 tcgtagtgtt ccgggaacgg accttccgcc attttgttga tggcaaacag gcgtcccatc
     5161 ccttccggct gcataataaa cggcccggtt ggcgtacccg gtgcggcatt gccgaagtcc
     5221 ggaatatcgt tacccgtcca cttgctgccg ttccactgga tcagcatccg tttcggatcc
     5281 cacggtttac cgttgatatc cgccgaagca cggttgtaga gtacgcgacg gttgagcggc
     5341 cacgcccagg cccatcccag cgtattcccc agaccggacg ggtcggagtt atcgcggttt
     5401 gccatctggt taccctgctc tgtccagcta ccggtgtaga tccagcaaga agatgcggtt
     5461 gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact gacctttctt cgcaatcagc
     5521 acgccattag catcatagag atcttccaac gcatagccgt tgttctcttt agctacttcg
     5581 tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag cggttctacg
     5641 cctttaccac cttcggcctg gtacaactcg cgcagatgat ggtagatacc cgccagaatt
     5701 tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag
     5761 cgaccggagt tagcgataga accatcctct tcagcaaagc aggtcgaagg cagacggaat
     5821 acttcagtct gaatagacgc cggatcgaca tcgttcgact cgccgtggtt ctgccagaag
     5881 gtagaggttt cagtcaccag cggatcgata accaccatgt acttcagttt gctcaggcag
     5941 ctcaccactt tgtttttatc cgggaaggac gcaaccgggt taaagccctg gcagaaataa
     6001 ccggtaactt tgccttcatc catcatgttg aaatacttga tgacgtcgta ggtctggtcc
     6061 cacttcggca gccagtcata gccccagttg ttctctttct gcgcggcatc gccatagaaa
     6121 gatttcatca ggctaacgaa gaacttcgga tagttgctcc agtagttcac ctgatcagcc
     6181 agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca aatcaacctg tttttctgac
     6241 ggcagcgtca gataacctgg caggctggta gagagcagac ctaagtcagt cagcccctga
     6301 atgttggagt gaccacgcaa tgcgttcacg ccgccaccgg ccatacccat gttaccgagc
     6361 agcaactgga tcatcgccat agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag
     6421 cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc tggtggaggc cagcacttca
     6481 cacactttca ggaagtcggc ttttggcgta ccacagatgt tttcaacaac gtccggcgtg
     6541 tagcgggaaa cgtgcgcttt cagcaggttc cacacacagc gcggatgagt cagtgtttca
     6601 tcgcgtttcg catagccgtt ttcatcgaac tgatagttcc aggacgattt gtcgtactgg
     6661 cgtttttcag cgtcataacc gctgaacagg ccatcttcga aagcaaaatc atcacgcacc
     6721 agtaggctgg cgttggtgta atgtttaacg tattcggcgt tgattttgtt gttttcgatc
     6781 aggtagcgca aaatgccaga caggaacgta atgtccgtac cggaacgaat aggtgcgtaa
     6841 atatccgcca cagaagcggt acgcgtaaaa cggggatcga caacgatcaa ggttgcatcg
     6901 ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta
     6961 ccgcccatta ccattacgac gttagcgttt ttgatatcca cccagtggtt ggtcatagca
     7021 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac gcgcgcctgg
     7081 ttgtctaccg ccagcatccc gagggagcgg gcaaatttct gggtcagcat cccggtttcg
     7141 ttgctggcac cggaggcaca cagcataccg gtagaaagcc aacggtttac cgttacgccc
     7201 tgctcgttct tttcaataaa gttagcgtca cggtcagctt tcatcagctt cgcaatacgg
     7261 gagaatgctt cttcccagct aatgcgctgc catttgtcag aacctggcgc acgatatttc
     7321 gggtagcgca gacggttttc actgttgacg taatccagca aaccggcccc tttcgggcac
     7381 agcgcaccac ggcttaccgg atgatccggg tcaccttcaa tgtgataaat cgcttctctg
     7441 gcgttttttg cgccatcacc caggctatac atcaatagcc cgcaacctac ggaacagtat
     7501 gtgcaggtgt tacggatctc tttagcgcgt aataatttgt agtttcgcgc ctgagccagt
     7561 gcttgcttcg gggcaaagcc caatgccgct accgttgttc cagccatacc gcccgcgcag
     7621 attttaaaaa attgtctgcg actgacgtcc attgctttcc tcttttttca ggggtattac
     7681 tgcgcgagga aactaacagt aaaattctga atctttttgc gcgaaatcaa aaagaacacg
     7741 tgtttgccac aaaataatcc gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac
     7801 gcaccactgc ctgacaggcc agttaaaaaa atgctataaa attcagctta atttttaacg
     7861 gcaagagaga caaaacagcg agcatgacac gacaaaaagc aacgctcata gggctaatag
     7921 cgatcgtcct gtggagcacg atggtaggat tgattcgcgg tgtcagcgag gggctcggcc
     7981 cggtcggcgg cgcagctgct atctattcat taagcgggct gctgttaatc ttcacggttg
     8041 gatttccgcg tattcggcag attccgaaag gctatttact cgccgggagt ctgttattcg
     8101 tcagctatga aatctatctg gcgctttcct tagggtatgc ggcgacccgt catcaggcga
     8161 ttgaagtggg tatggtgaac tatctgtggc ccagcctgac aattctcttt gccattctgt
     8221 ttaatggtca gaaaaccaac tggctgattg taccaggatt attattagcc ctggtcggcg
     8281 tctgctgggt gttaggcggt gagaatgggt tacattatga tgaaatcatc aataatatca
     8341 ccaccagccc attgagttat ttcctggcgt ttattggtgc gtttatctgg gcagcctatt
     8401 gcacagtaac gaataaatac gcacgcggat ttaacggaat taccgttttt gtcctgctaa
     8461 cgggagcatg tctgtggatt tactattttc tcacgccaca accagaaatg gtatttagca
     8521 cgcccgtcat gattaaactc atctctgcgg catttacctt aggatttgct tatactgcat
     8581 ggaatgtcgg tatattgcat ggcaatgtca ccataatggc ggtaggctcg tattttacgc
     8641 ctgtactttc ctcagcgctt gcagccgtgc tgctcagcgc cccgctgtcg ttctcgttct
     8701 ggcaaggcgc actaatggtc tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg
     8761 gttaaaataa ttgccgggtc tacggcccgg cagttaatca gataaattcc gacaatatta
     8821 aaaaataaat ttcaaatacc agtaattcac cttatatctc atttcgactt cgatcacata
     8881 tcgataaata atattattct atttttctca ttgtttttaa tcaccattct attttaaaaa
     8941 cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata
     9001 attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt
     9061 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct
     9121 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
     9181 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
     9241 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa
     9301 caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg
     9361 tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg
     9421 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac
     9481 ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca
     9541 aggcaaaaat aaacgtactg acaacagtca tctttatggt gctgacgaca cgcgtgccaa
     9601 cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
     9661 atataccaaa tccgatcgga caaatgcgca ggaaagagcc gcagctaatc ctctcaatgc
     9721 ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta
     9781 ctttgcagct aattacgatg aaacattaaa catgaccacc tatggggatg gttatatctc
     9841 taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc
     9901 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacgccg accaggacat
     9961 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
    10021 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcgcac
    10081 cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc
    10141 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
    10201 attcttgata tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat
    10261 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
    10321 ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt
    10381 tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa
    10441 ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
    10501 atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa
    10561 acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca
    10621 gcgttaataa aattaacacg ataacctttc caaaagaacc aaaactggaa aacatttata
    10681 ttaatcataa caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg
    10741 aagcgcaaaa taatagccta aaaaaatgat atttctgata gttataaact gaaatttctt
    10801 aatcttgatt ataacaagct agcatcgctg gatgtctccc ggcaagaatc tctgattgag
    10861 ttaagtgccc atcacaatat gatcactgac cttatattac acaatcatcc caggatgaaa
    10921 acaatcactt taaacgacaa ccatattgca catttaaacg ccaaaaacac tacaaaactg
    10981 gaatatttaa acttaagcaa taacaattta ctgccaacca atgacattga tcaactaata
    11041 tcatcaaaac atctttggca tgtattagtt aacggcatca acaatgatcc acttgcacaa
    11101 atgcagtact ggactgcagt aagaaatata attgatgaca ctaatgaagt gaccattgat
    11161 ttatcaggac ttaatttaac cactcaacca ccagggctgc aaaacttcac ctctatcaat
    11221 cttgataata accaactcac acattttgat gcaaccaact acgatagact cgtaaagcta
    11281 agtctgaata gtaatgctct tgagtcaata aattttcctc aaggcagaaa tgtaagtatt
    11341 acacatatat ctatgaataa taatgctctc agaaatattg atatagatag gctttcatca
    11401 gttacttatt ttagtgcggc acataatcaa ctagagtttg tgcaattaga atcttgcgaa
    11461 tggctgcaat acctgaatct cagccataat caattaactg atattgttgc aggtaataaa
    11521 aatgaactct tactgctgga tctatcccat aataaactaa caagtttaca caatgtctta
    11581 tttcccaact tgaatacgtt acttattaac aacaatttgc tttctgaaat taaaatattc
    11641 tatagcaact tccgcaatgt tcagacatta aacgctgcta acaaccagtt gaaatatata
    11701 aatcttgatt tcctgactta tcttccatct atcaaaagtt taagactgga caataataaa
    11761 ataacccaca ttgatactaa taatacatcc gatattggaa ctttattccc cataataaaa
    11821 cagagcaaaa acttaaattt tttaaatgtt tctgggaaga acaattgacc tactatgcag
    11881 ctcatgttat ttaatttgtt ttccccagca cttaagctta atactggcct ggcaattctt
    11941 tcgcctggtg catttgaagt tcactctgac ggaatagatg cggataacga attgtttcac
    12001 tatactatta aaaaagcata taccccatat aatatacaca cttacaagac agaggaagtt
    12061 gtaaaccaga ggaatataaa agttaaa
//
LOCUS       DAJTNP010000106        12035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTNP010000106 DAJTNP010000000
VERSION     DAJTNP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..442
                     /locus_tag="OO160_003300"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO160_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1027..1743
                     /locus_tag="OO160_003301"
     CDS             1027..1743
                     /locus_tag="OO160_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525306.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2295..2699
                     /locus_tag="OO160_003302"
     CDS             2295..2699
                     /locus_tag="OO160_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525307.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2762..3388
                     /locus_tag="OO160_003303"
                     /pseudo
     CDS             2762..3388
                     /locus_tag="OO160_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3418..6330)
                     /locus_tag="OO160_003304"
     CDS             complement(3418..6330)
                     /locus_tag="OO160_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525308.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            6333..6449
                     /locus_tag="OO160_003305"
     CDS             6333..6449
                     /locus_tag="OO160_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5525309.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            6813..7655
                     /locus_tag="OO160_003306"
     CDS             6813..7655
                     /locus_tag="OO160_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5525310.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            7658..8746
                     /gene="capU"
                     /locus_tag="OO160_003307"
     CDS             7658..8746
                     /gene="capU"
                     /locus_tag="OO160_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5525311.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            8751..9701
                     /gene="virK"
                     /locus_tag="OO160_003308"
     CDS             8751..9701
                     /gene="virK"
                     /locus_tag="OO160_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5525312.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            9766..10710
                     /gene="lpxM"
                     /locus_tag="OO160_003309"
                     /gene_synonym="msbB"
     CDS             9766..10710
                     /gene="lpxM"
                     /locus_tag="OO160_003309"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5525313.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(10864..11895)
                     /locus_tag="OO160_003310"
     CDS             complement(10864..11895)
                     /locus_tag="OO160_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5525314.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSVGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT         3212 a         2668 c         2830 g         3325 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaatactgg attgtgggcc ataaatattc caaaaagata
      481 gcccgctccg tttattaccg gaacgggcga gagcatgaca ttgtctgtta ttataatgag
      541 actgctgatt ctacttattt ttaatttcaa gaaaagcgtt ttctacaaag aattatgaga
      601 tttaatgttt tgtttagaca atattatcat tctgttcaat aatgaaagtc ttcatcaaca
      661 acaagtaaaa acaacgtccc tctcatcgaa ccggacttgc agatttcccg catccggctc
      721 tcttgcaaca catcagacct tcacgttcgg gcggttatgg ggaaggtcca gcaagctgta
      781 tgagccctaa ctgtttgaat accagtagat taccagaaac ttctatcgat tgaagacatt
      841 accaactagc gttactaaat gttgaccaag ttttgttcac ttgtctggtt ttgatagtag
      901 cattctgcag agtattttga tatgataaat atgcattaaa aatgatatta ttgttaagtt
      961 cctagcatga aaattaaaag atcaactttt atctcgaata ttttttacat tatcagttag
     1021 tttcttatga atgacaattc attgcttaga aattcttcat tattcatcgc ctatatggga
     1081 tgtgtagggt gggtcagtgc ttattcttat ggctggggaa cctcttttta ctatggattt
     1141 ccatggtggg ttgttggtgc aggtttggat gatgttgcca gaagtttact ctatgctatc
     1201 atagtaatgg gtatactttt taccggttgg gggattggta ttttattttt tcttcttata
     1261 aagaaaagaa gtaaaataca agacttgagt ttttttagac tattttttgc tattacccta
     1321 ctgtttttcc ctgtaatatt cgaactttta attctcaaac aatatttcat acttccatta
     1381 tcactttcat tcattattag ttcattggtt atttcaataa taattcgtat atatggacgg
     1441 atcttttctg tatcatgttt ttcagatatc ccttttgtta gagaacaccg cattaagtta
     1501 attatggctg gttttttggt ttatttttgg tttttttcct ttcttgttgg ctggtataaa
     1561 cctcaattaa aaaaagaata tcagatgctt tgttataata atagttggta ttatgttctt
     1621 gctcgttatg attcacggct cgttttatct agttcattta aagatgacag taacagattc
     1681 ttaatattta atacagaaca gtcgggattt tatgaaatca atgatgttta tgtcagaaag
     1741 taataatcct ctcccacaaa gtgaattaaa tgaatatata atgcgttcga agtatatgta
     1801 ttgagctgtc atactgagaa acaaacctta gggcagcaca attaaagcca gaaacaacta
     1861 tgcaagaaag ttactaaata agtgctcgga acagtaaaac actcaggggg gactcagtcc
     1921 ggattatgta atcagataaa ttgccaaaca tcacaaatca ccagccggac tgagtgatgc
     1981 cgagcattgc accaataccc cgtgatgaac gatgcctgat gcagaaagct ccctataaaa
     2041 caaagaacaa aaactacgac cggcgattca ccgtcatact gatgctgcat cgtggtgaca
     2101 ctgtctgtca ctttcatgtt gactgaacaa ctctgctgcg aagaaggtgt accgccggcg
     2161 ggatttgatt gtccggcgtc tagcttccac ttccctgatg gaactgaaag ttgttatggc
     2221 cggtcagctt gccctgtccg caggacatga ggtgctgtga cggacagcac cagtaacgga
     2281 aagtgacaat gaaaatgaat actgcgctga tagttgcact aatgtgtatg tggtatgcgg
     2341 tacccgctgc tgcaaaagag actttactgg ctatgccacg taattcaaca gaacattgct
     2401 atgcggagat taatgtgcac ggaccatatg gggtgtattt ccgagtggtc ccgcatcccc
     2461 ccgggggaaa gagctgggtc gaatgtaaca gtgactacta ttactctgat aaaccaccgg
     2521 gagtacagat actcggaacc cgtgcagggt gtcgggtata cggaatctgc gggaccacct
     2581 cgacactgca tgtggctgga agaggggttg tgtgtattaa aaatatatgc agtccacgtg
     2641 ggatgataat tcacaggata cggaaacgtc ctgtagtggc tgtaagcgat gagatgtgat
     2701 cttacccagc aatagtagac acgcggctaa gtgagtaaac tctcagtcag aggtgactca
     2761 catgacaaaa acagtatcaa ccagcaaaaa aacacgtaaa cagcattcgc ctgaattttg
     2821 cagtgaagcc ctgaagcttg ctgaacgcat cggtgttgct gccgcagccc gtgaactcag
     2881 cctgtatgaa tctcagctct atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc
     2941 tgagcgtgaa agtgaactgc ccgcctgaag cggcaactgg cggagcaggc agaggaactg
     3001 gctatcctcc aaaaggccgc gacatacttt gcgcagcggc tggtatacgt ggtgtcagcg
     3061 tcggacagga ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttgtcctcgc
     3121 ggcggccttt acccggtcaa aacagcgtta cggtgcacca cgcctgacgg atgaactgcg
     3181 tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct
     3241 gagggcaaag gcctcccgga agttcagccc ggtcagttac cgcgcacatg gcctgcccgt
     3301 gtcagaaaat ctgctggagc aggattttta cgccagtggt ccgaaccaga agtgggcggg
     3361 agacatcacg tacttacgta cagatgaagt tcggttacat cctgtcagca cggaacctca
     3421 cgctttttga gcggcacgac ggatcccccc cacccagtat ccgacagact tttcactgcg
     3481 ttcgtaaaaa cccttcttcc ctcccagcat atccactaat tccggtgaca tatcctgcag
     3541 ataagtctgc aaatcatgcc ccagatcttc cggtgactgc aactcataaa ccgcgcgcca
     3601 cacatcaccc ttccttattt taagccccgg gttaagaata tactgtccgt ggttcagcct
     3661 cagaaaaagc ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct
     3721 gctgatataa ctccttcgtt tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac
     3781 acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg
     3841 cgtgtggaac agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag
     3901 tttgatgagg cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa
     3961 ctgcgcactt ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg
     4021 ataagccgcc agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa
     4081 ctgaaccagt gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg
     4141 cgaacggtga tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat
     4201 tgagtatggc cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct
     4261 tgtgggctta aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag
     4321 actgtactcc agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg
     4381 tttccactgt cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag
     4441 gatccggcta cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc
     4501 ggttttctgc cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg
     4561 atgcgtgagc gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac
     4621 attgtgttta gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc
     4681 cgctgaacga tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc
     4741 cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta
     4801 aaggatcacc gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt
     4861 actgaaccag catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg
     4921 attcgaaagt gaggttttgg cattcagttc ctgctttgtc tcttccctat cgtccagcaa
     4981 ccggatggtc ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat
     5041 agcactaaag cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg
     5101 cccgttacgg taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg
     5161 gaaaaagaga cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc
     5221 atcaccacag agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg
     5281 aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa
     5341 cgaaagatac tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc
     5401 gatataacgt tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg
     5461 ccgtatcccc agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc
     5521 accgatcacg cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc
     5581 cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc acctccctgc cttccggaat
     5641 gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca atattctgta cctcactccc
     5701 ttcccctgaa gcgtcataga gagtacgcgc tttttccacc agaaatgagg aaaggctgag
     5761 atacagtata tcaccagcag cctgcttcat tttttccagt accagcgccg tcttaccact
     5821 tcccgccgag ccaacaatca ccagcggtag cgggtgttcg tacagtgcct gctgatcatc
     5881 atcaaaggac agcattttat ccaggcggtg gaaacgccca tgagacggat tgatatacgt
     5941 cagcgcttca gtcgcgatat caacaggatc cggcactggt tgttgttgta agcgcccttc
     6001 atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta taggcatgat tgcgaatata
     6061 ttccagaaca aggcagatag tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc
     6121 ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac
     6181 aaagttatcg gtggcaatag cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt
     6241 cagagtatga taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct
     6301 gattttacga aggaatgcct tactgaacaa ccttgcccgg aatacccccc gaaatcagat
     6361 ggaacaatca gaaaaatgct ctgcagttat ggaataaaga aggtggggtg gtacgccggg
     6421 agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag
     6481 ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt
     6541 ttttcattgg agtacctgtg ttgttctgag gtgaacatat cacctctgtt caggtagcca
     6601 aattcagtgt gccacctcaa ggttcttagt gagtcgtttt taaaagatat gataatatat
     6661 cattgtttat gaattgttga tgtttaattt agtaaattat atcaaatatg ccatgggttt
     6721 gctatgttat tcatccagta tctggaaata aaatgcacca gctttattgg gttttccttt
     6781 tgtctctgct tgctaggtgc cagaaacttt aaatgttaaa cgaagggggc attttgttca
     6841 aagccaatca tgttccggta ttaatgtatc atcatgtcag tcattgcccc ggactggtta
     6901 cactttctcc cgtgacattc cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa
     6961 cactgtcatc agatgaactg gagttttttt atcggggcgg aaaactcccc cggaaaagcg
     7021 ttatgttgac atttgatgat ggataccttg acaactggtt tcaggtatat cctttattaa
     7081 aagagtttaa tctcaaagca catatttttt taattaccgg ttttattggg aatggtccgg
     7141 ttcgtcactc tcctggaaag gaatattctc atcgcgattg cgaacatcaa atagcgacag
     7201 ggaatgctga taatgttatg cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc
     7261 tggtcgaatt tcatgttcat acgcatactc atacacgctg ggataaaaag ttcagttcac
     7321 gagaagaaca gtgtaaacat cttcggcagg acttgttgag cgggagggaa tatttgaaag
     7381 aaatgacagg aaaatgcagt aaacatttgt gttggccgga aggttattat aacaaggatt
     7441 atattcaggt cgctgaagaa cttggattct attatttata tacaacagaa agaagaatga
     7501 atgctcctgc taaaggaact acacggatag gaagaataag caccaaagaa cgggaaagtt
     7561 gcgcatggct gaaaagacga cttttttatt atacaacccc gtttttttca tcgttgctgg
     7621 cctttcataa ggggccaaga ttaccggatg attaataatg aatatactat ttacggaatc
     7681 atcaccgaat ataggtggtc aggaattaca ggctgttgct caaatgaagg ccctgaagaa
     7741 aatggggcat tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag
     7801 taaattgggg attgatatca cattcgcgtt atttcgaaac agtcttcaca tccctactgc
     7861 atggagatta ctcggaatag ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg
     7921 gcatgatagc aatattgttg gtttagtacg gttattcact aggaaacatc cattcagaat
     7981 tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt ttttcaataa atcatttttg
     8041 cgatgaggtg attgttcccg gaacaagtat gaagacacat ctggagcagg aaggatgtcg
     8101 aacccgggtt actgttgtgc ctccaggctt tgatttccag aaattatacg ttgattcccg
     8161 aaacagtttg cctccaaatg ttctttcttg gctggcgtcc cgaaggggat gccccgttat
     8221 tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa tttatgttga atttactgtt
     8281 ccatttaaaa atgaatggac gacagttctg ttggttgatt gtggggtctg gttcgcctga
     8341 actgcgggag catttacagt atcagattga cagtatgggc atgcatgatg atgtttttat
     8401 tgctgacaat gtttttcctg ccgcccccgt atatcgggtt gccagtctgg tggttctgcc
     8461 ttcagaaaac gaatcgtttg gtatggtact ggcagaagca tcggcatttt ctgtgcctgt
     8521 actggccagt cagattggtg gaatccctga tgttattcag aataaccaga ccgggacatt
     8581 gttaccagca ggtaataagc acgcatggat gtgcgccctg aatgattttt ttaatgaccc
     8641 tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat atagaagagc ggtttgatat
     8701 taataaaact gcgttaaaaa tactcacatt agcgaagcac aagtaacaat atgttttctg
     8761 taagtaactt atcatttatc ggtttcctta aaaggattgt tttttcctca gattcactcc
     8821 cggggaagtg ggaacacaga aaattccggt tcatgtacat tttgcgatgt gctataaatc
     8881 cggttgccag tattcgatat tattacgaac tgcgttcctt gcagtgcatt gaggatattc
     8941 tggcaataca acccacgttg ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc
     9001 gagcctggtc ccgggggcaa tatattcttg agcattatcg ctttgtgcaa aatttgccag
     9061 aaaaatattc tgaatttttg tttccacaaa aatcggtatc attagtccaa ttcatcggga
     9121 aagatggaga ggattttgat atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt
     9181 tgatgttgtc attgtttttc aataaaatag tgattgcccg gttaacattt tcagtcattc
     9241 tgactcaaaa tggacacacc gcttttattg gcggattaca gggagccccc aaaaataccg
     9301 gacctgatat tattcggtgc gcaacccggg cctgttatgg gcttttccca aaacggatta
     9361 tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca
     9421 gtgagcacag tcatgttttc cggcaattga gatactggta tcagaaacgc aaaacctttg
     9481 ttgctgtcta cagtgatttc tgggagtctg tagcagggaa aacctgtggt gactggtaca
     9541 aattaccaac acaggtggtt cgaaagccgc taagcaatat agctagtaaa aaacgctctg
     9601 agtatcgaaa acgatacgct ttgctggatt atatccatga aactgctatc cgctctttgg
     9661 atgcatatcc tgtaaactca gaacatcagg acttaaatta aggtttgatg tattctctga
     9721 ataaaatatt aatgatgatt ttggtagggg cattcgcact aaataatgaa aaaatacaaa
     9781 tccgagttta ttcctgaatt taagaaaaat tatctttccc ctgtttactg gtttacatgg
     9841 ttcgttttgg gaatgattgc aggtatttca atgtttcccc cttcattcag agatcctgtc
     9901 ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg
     9961 attaatttat cgctttgttt cccggaaaag agtgatacag aacgggaaat aattgtcgac
    10021 aatatgtttg ccacagcatt gcaatccata gtgatgatgg cagaactggc aattcgtggt
    10081 ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg aaattcttga ggaaattcgg
    10141 cacaataaca gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca
    10201 atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca
    10261 gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt acattcccgg
    10321 gaggatggga taaaaccatt tattcagtca gtacgccagg gatactgggg gtattacctt
    10381 ccagatcagg atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa
    10441 gcgacattac caattattgg acgtctgatg aacatcagtc aggctatgat tataccgctt
    10501 ttcccggttt atgatgaaaa aaaacatttc ctgactattg aagttcggcc accaatggat
    10561 gcatgcattg ccagcgcgga caataaaatg attgcccgac aaatgaacaa aacagtggaa
    10621 attttggtgg ggtcacatcc ggaacagtat atctgggttt taaaattgtt aaaaacgcgc
    10681 aaatcaaacg aagcggaccc gtacccttga aattataaag tacaggtatt tccactagtt
    10741 gtttcttaca ggttaccaat cgaaacacat cccccttccg tgtgttgccc gtttggcacg
    10801 gaggactgtg tagatgcagt tcgttaaatt taatttatgc ctaaatagct gcgcttaata
    10861 ccgctacact tttgccagcc catgtttgcc tccggggaat gggctgacgg tttccataaa
    10921 atggcgaact tttttcaaca gttgccacat tgagcggcac tgatgattac gcgttattgt
    10981 gtcgtgaagt gcctgccata gccgttcaac atgattcacc catggcgagt aaaccggctg
    11041 ataaatgccc ctgaacttcg ggttctcctt cagccagctc tgtgtttccc ggcttttgtg
    11101 gataatgtag ttgtccacga tcagcgtgat ggttttcgca cgacggtatg tcgctttaag
    11161 ccgcttcagc aggctgatga acagcgccga acttttgctg ttgccgccca catagctgac
    11221 tttacctgtc ccgctgtgca gcgctccggc cagataatat ttttcattct gtcccggcgt
    11281 gaccacccgt ttttgctgtc cgcgcagttg ccagtccgca ccgattttgg gatgaagatg
    11341 gatatccact tcatcttcat aaaagaccgg atgctctgcg ctgcattcgt ccagtgcttt
    11401 atggattgct gccatctttt catctttatg cgggtcacgg atacgcagag ttggcgcagc
    11461 ccttcgccac acaatcccca cagacggcaa ccagcggcga acggttccgg catttaactg
    11521 gcaaccggtt atctcattga tttttattgc cagcagttct gtactccagc gtgaacgctg
    11581 gtagccaaag tcgccgggag aatgttttac cagctcacgt aacagtgtgc agatatgctc
    11641 aaacggccag cgacgggcac gcccggcagg taatgatttc agtccctcaa cacccgactg
    11701 cgtgaaccag ttaatccagc gtccaacaga ggaacgggcg cagcagagcg ttctggcaac
    11761 gtcgctgaca cggtcgcccc ggtgcagcat cagcatggca gtcagtctgc gggcataatt
    11821 tttatcgtgt gttttatgga tggctttctg catcaggcgt cgttcgtcac gggaaattgg
    11881 tgctatgatc ggcattgctc agtccggttg gtgatttgtt ttgatttggc gattgatcag
    11941 atcgcacaat ccgggctaag ttccctcaaa gggatctact attccgcgca gctatttagg
    12001 ctgtgtccct taattacgta agcgttgata aaaga
//
LOCUS       DAJTNP010000107        11927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTNP010000107 DAJTNP010000000
VERSION     DAJTNP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..2342)
                     /locus_tag="OO160_003311"
     CDS             complement(<1..2342)
                     /locus_tag="OO160_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5525315.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO160_003312"
     CDS             complement(2276..2899)
                     /locus_tag="OO160_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5525316.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO160_003313"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO160_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO160_003314"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO160_003314"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5525317.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO160_003315"
     CDS             7507..8229
                     /locus_tag="OO160_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5525318.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO160_003316"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO160_003316"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5525319.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OO160_003317"
     CDS             complement(10301..11896)
                     /locus_tag="OO160_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5525320.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3309 c         2946 g         2915 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcggcg
//
LOCUS       DAJTNP010000108        11868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTNP010000108 DAJTNP010000000
VERSION     DAJTNP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(214..858)
                     /gene="cheZ"
                     /locus_tag="OO160_003318"
     CDS             complement(214..858)
                     /gene="cheZ"
                     /locus_tag="OO160_003318"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5525321.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(869..1258)
                     /gene="cheY"
                     /locus_tag="OO160_003319"
     CDS             complement(869..1258)
                     /gene="cheY"
                     /locus_tag="OO160_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5525322.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1273..2322)
                     /gene="cheB"
                     /locus_tag="OO160_003320"
     CDS             complement(1273..2322)
                     /gene="cheB"
                     /locus_tag="OO160_003320"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5525323.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2325..3185)
                     /gene="cheR"
                     /locus_tag="OO160_003321"
     CDS             complement(2325..3185)
                     /gene="cheR"
                     /locus_tag="OO160_003321"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5525324.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3204..4805)
                     /gene="tap"
                     /locus_tag="OO160_003322"
     CDS             complement(3204..4805)
                     /gene="tap"
                     /locus_tag="OO160_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5525325.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4851..6512)
                     /gene="tar"
                     /locus_tag="OO160_003323"
     CDS             complement(4851..6512)
                     /gene="tar"
                     /locus_tag="OO160_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5525326.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6657..7160)
                     /gene="cheW"
                     /locus_tag="OO160_003324"
     CDS             complement(6657..7160)
                     /gene="cheW"
                     /locus_tag="OO160_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5525327.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7181..9145)
                     /gene="cheA"
                     /locus_tag="OO160_003325"
     CDS             complement(7181..9145)
                     /gene="cheA"
                     /locus_tag="OO160_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5525328.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9150..10076)
                     /gene="motB"
                     /locus_tag="OO160_003326"
     CDS             complement(9150..10076)
                     /gene="motB"
                     /locus_tag="OO160_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5525329.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10073..10960)
                     /gene="motA"
                     /locus_tag="OO160_003327"
     CDS             complement(10073..10960)
                     /gene="motA"
                     /locus_tag="OO160_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5525330.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11087..11665)
                     /gene="flhC"
                     /locus_tag="OO160_003328"
     CDS             complement(11087..11665)
                     /gene="flhC"
                     /locus_tag="OO160_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5525331.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11668..>11868)
                     /locus_tag="OO160_003329"
     CDS             complement(11668..>11868)
                     /locus_tag="OO160_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5525332.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2704 a         3338 c         2887 g         2939 t
ORIGIN      
        1 tggcatcact acgatgtact gcactaccag tcatcgtcat tttctcgttc acaatttaat
       61 gcattgataa atttactggc gcaacaatca cgcttcgctg atctaaaatt atcaaactac
      121 aaataaccat cctccacgta ctccctgaac gatacccgcc accctgctat cggcttggca
      181 tcatcgcgca ggccgaagtt caggcaatac aaatcaaaat ccaagactat ccaacaaatc
      241 gtccacctga tcctgactgg ctaccacgcc ggctttgctg gtatcgacct gaggtccatt
      301 aagcaaactc tggttttcac gttttggacg cgactcctgt tccgggatgt tttccaacag
      361 caccatcagc aactggcgtt cgatctcctg aatgacatcc atcatccgct taataacctg
      421 cccggtgaga tcctgaaaat cctgcgccat catgatttcc agcagttgcg cgttagtaaa
      481 gctggtatgc gcgggtacat ctgccagaaa ttgtcgtgta tctgttacca gttcacgggc
      541 gtcggcaagg tcaatcggat cggcaaacca gtcatcccaa cgttgggtta acgcttttgc
      601 tgatttctcc atttgatcct gatgcggttg tgacgcctca acactgttca gcgcccgctc
      661 cgcagcctgg gcggtcatct gcacaacata gtacaaacga tcgcgcgcat cggggatggc
      721 ttccgccgct tcggcaatgg cctgaaccag ccccagttcc cgcaaactgt cgcgcagcat
      781 atgcgtcagg ctgccgatgc gcgcaatgat atcgccagct gaatgctcgt cagcaggttt
      841 gattgatggt tgcatcatag tcgcatcctc acatgcccag tttctcaaag attttgttga
      901 gtttttcctc cagcgtcgcg gcggtaaatg gcttcaccac atagccactg gcccccgctt
      961 gcgccgcagc aatgatgttc tctttcttcg cttctgcagt caccattaac actggcaatg
     1021 ccgacatcgc gccatccgca cgaattgttt tcagcaattc caggccatcc atattgggca
     1081 tgttccagtc ggagataaca aatccataac cgcctgcctg caacttattg agagcgtcga
     1141 caccatcttc cgcttcctca acattattga atcccagctc tttcagcagg ttacgcacta
     1201 tgcgtcgcat ggtggaaaag tcatccacaa ccaaaaattt aagttcttta tccgccattt
     1261 cacactcctg atttaaatac gtatcgcctg tccggcacta attgttgcca acatttgctg
     1321 gcttacctgg ctaagatcga ccacttcgca gacaccaccc atattgatgg cctcgcgcgg
     1381 catgccgaac accacgcaac ttgcttcgtt ttgcgcaagg gtccatgccc ccgcctgacg
     1441 catcgccaac attcccgccg cgccgtcgtt gcccataccg gtcaggatca ccccaaccgc
     1501 attacgcccc gcctgtttgg cgacagaatg gaacaacaca tctaccgaag gccgatgacg
     1561 gttaaccgcc gggccgtcgt gaattttgat ttgataattt gcgccactac gcgccagttc
     1621 catatgccga tcgcccggcg caatataggc atgccccggc aagacacgtt ctccgtcttc
     1681 ggcttcttta accccgatct ggcaaagctt attaagtctg tcggcaaaag agcgggtgaa
     1741 accgggcggc atatgttggg taattaacaa tgccgggctg gaaagcggca acggttgcag
     1801 tacgtgacga attgcctcag ttccacccgt tgaagcacca atcgcaatca gtttttcaga
     1861 actcaacaac ggccctgcct tcagcgttgt cggtgctgac aatggcttat gtgctgcaag
     1921 gctcgccttt gctgccgtac gcaccttttc agcaatcatt tcgctatacg ccagcattcc
     1981 ttcgcgaata cccagttgcg gtttggtgac aaaatctatt gcccccagct ccagcgcgcg
     2041 cagcgtgact tctgatcctt tgccggtcaa ggaagaaacc atcacaacgg gcattggacg
     2101 caaacgcatt aatttttcga ggaaatccag tccgtccatc cgcggcattt caacatccag
     2161 cgtcagcaca tcgggattga atttcttaat caagtcacgc gcaaccagcg gatcaggcgc
     2221 ggtcgccacc atttccatgt cgctatggct gttgatgatt tctgtcatga tctggcgcat
     2281 cagtgccgaa tcatcgacag ataacaccct gattttgctc atcgttaatc cttacttagc
     2341 gcatacaccg tctgaccacg cagcgtgaag cggcgctcga ggtggctaaa gttttcagag
     2401 tgacccgcaa acagcaatcc gtcgggttta aggagcggaa caaagcggcg caaaatctcc
     2461 tgctgggtag tttgatcgaa gtagatcatg acgttacgac agaagatcgc atcaaacggc
     2521 cccggcacgg tgtactgttt cgccagtaaa ttcagcgggg caaaatcaac atagttcgcc
     2581 agctcctgac gcacgcgtac cagcccttca tgcggtcccg tccctcgcat gaaataccgt
     2641 tgcagttgct gcggcgtcag gtttttcaac tcttcatggc gatagatacc gcttctggct
     2701 ttttccagca cttcggtgtc gatatcactg gcaaacactt tccagcgtcc gggcacggtg
     2761 cccaatgtgt cagccagagt catcgcaatg ctgtacggct cttcgccggt cgaagccgcc
     2821 gcgctccata cgcgatcctc gccagaacgg cgacgtgcgt gatccgcgag cagagggaaa
     2881 tgatgagcct cacggaaaaa tgccgtcaga ttcgtggtca gcgaattgat aaacgcctgc
     2941 cactcaccgc tgtgctgatt agattccagc aagttcagat aatgaccgaa atccgtcagt
     3001 cccagagaac gcaaacgacg aaccagtcgg ttgtaaacca tgtcgcgttt atggtcagcc
     3061 agaacgatcc cggctcgttg atagatcaat tgacttatcc gccgaaaatg cgcgtcggaa
     3121 agcgccaggc gctcggtcat ctgtaacaat aaagacgttt gcccacaggg cagagatgaa
     3181 gtcatagcgc cttctcaatc acttcaggat accactggcg caatttgtaa ctgcgccgac
     3241 tcatgtcgtg ctacttcatg ttcttcaagg gtaaataccg ccacacgcga cgaaagatgg
     3301 tcggcctgat tcgccagttg ttccgttgcc accgccgctt cttctaccag cgaggagttc
     3361 tgctgagtca cctgatccat ctggctgaca gcctgtgcaa cctgctcaat cccccgccgt
     3421 tgttcttccg acgccgaagc aatttctccc ataatgtcgt tcacgcgggt caccgaactg
     3481 acaatatcgg tcatggtcgc ggcggcgtta ttcaccagtt tcgaaccctg ctggacacga
     3541 ttgactgact cttcgatcag ccctttgatc tcttttgctg cctgcgcgct acggctggca
     3601 agattgcgca cttcacctgc cactaccgca aaaccacgcc cctgctctcc ggcgcgagcc
     3661 gcttccactg ccgcattcag ggccagaata ttggtctgga aagcaattcc gtcgataacg
     3721 ctgataatgt cgccaatttt ttgcgagctg gtggcgatct cctgcatggt gtgagtcatg
     3781 gtactgacct gaacaccgcc cgcctgcgcc gttgtcgcgg cattttttgc cagttccgac
     3841 gcctgtcgtg cgttatcggc gttttgccct accgtggcgg ttaattgctc catactggcg
     3901 gccgtttgtg ccagcgatgc cgcctgctgt tcggtacgac ttgagagatc gttattgcct
     3961 gcgacaatct ccgcgatccc aatgtgcatc tcatggcttc ccttacgcac atcactcacc
     4021 gtcccacgca aagcctgctg catggtcttc aggctggcaa aaatggcggt gatctcatta
     4081 cgaccatata ccgcaatcgg acgcgccaga ttacccgcag caatgctgtc aaaatggcta
     4141 ccgataatgg ccagtggttg aacaatcatc ttgcgcgtcc accacagcgc actgctgatg
     4201 tagatcgccg caacaataat catgctgata aacaccagcg ccgaaatctg atagttacgc
     4261 tggctttgcg cactggcggc ttccagcaca tggttgatct ccagctgcca ggcctcaaag
     4321 ttaacgtcaa acgcattctg tgatccctgc accggcgcag tgaggaaatc cgaaagttga
     4381 ttactttcaa gccaggtcgc ctgatgttcg agatcgttgt gccagcgggc aaaacttttc
     4441 tccgtctctt tttgcaatgc cctgacgtgc tcgttgcccg cagtcatcgc cataaaactt
     4501 ttaaacagcg tggtggattg cgtcagactg gcgcgcgccg tcgtcatcaa cgttttaatg
     4561 tcatccgccg gatagctaag cgctgtcaga gtgcctgctt tgttcagcgc ggtactggcc
     4621 tgtaacatta ccgcccgagt ttgcgctaat gccgcacgtt gctgattgct ctgctcgccg
     4681 tgattcagtc gttgcaaatc gtcgcgaaat gcccaaaaag acatgccgtt actgccaatc
     4741 tgcaagatcc cgcagagaat caaaattaaa aacagcgtgg tcgaaattcg aatacgatta
     4801 aacatcaacg ctccccatca ggcggcaatg accgcgttag taaaaactcg tcaaaatgtt
     4861 tcccagtttg gatcttgttc agtaattcgc agtcgtggct gtgccggtgg ttgctcactg
     4921 gcaggacggg atggtgtttg cggtttattg gtgagtgggc tggctgccag acggaacgcg
     4981 gaaaccgctt gcgttaaacg actcgcctgt tcttccagcg cagctgcggc ggcagctgat
     5041 tcctgcacca gcgatgcgtt ctgttgcgtg acgcgatcca tttccgaaac cgccaatgcg
     5101 acttgatcga tgccacggct ctgttcatcc gatgccgatg caatctcgcc cataatgtca
     5161 gtcacgcgag tgacagcatt gacgatattg ttcattgttt ccccggcgct ttcgaccagc
     5221 accgaaccgg tatcaatgcg cgagacggag tcttcaatga gggctttgat ctcttttgcc
     5281 gccagggcgc tgcgactggc aagattacgc acttcacccg ccaccacggc aaaaccacgg
     5341 ccctgttcac ccgcacgcgc ggcttcaacc gcggcattca gcgcgaggat attagtctgg
     5401 aaggcaatac cgtcgataac gctgataatg tcggcaattt tcttcgaact atcggcgatc
     5461 tcatgcatcg ttttcactac gccatccacc actttgccgc cgtgctgggc ggtgtcggag
     5521 gcactttgcg ccagttgcga ggcctggcgg gcgttatcgg cgttttgctt cactgtcgcg
     5581 gtgagctgct ccatgctggc ggcagtttct tccagcgcgg atgcctgctg ttcagtacgg
     5641 gaggaaagat cggtgttgcc cgccgcaatt tcacgggtac cggcatagat ggcatctgaa
     5701 ccttcgcgga catgagtgac ggtgtcagtc aaagagcgtt gcatatgtga aacgctctgc
     5761 gccaggtcac ccatttcact gcgcccgtca atggtcaggg tattcgccag gttaccaccg
     5821 gcgatttcgc aaatgtgagc aataattttt gccagcggag taagcaacat acggcgaatg
     5881 ccgtaccacg ccaccagcag aatcaatacc accaccagcg cgataaccgc cagttgccac
     5941 tgggcaaatc ggtaatcatc tgcgttgtca gtgacgatat cgcgatacag tttttcactg
     6001 ctgagggcgt actgagcaaa cgcttcgccc attgcatttt gcattccctg ggttggctga
     6061 gcgaaataag ctccagtatt gccataatca agataatcaa tcagttcagt taacgctgtg
     6121 taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc aggtaacggt
     6181 gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt cctggcgcta
     6241 tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat catccgcacc
     6301 gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga cgtcagctcg
     6361 ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg gtgaagggaa
     6421 gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag caccattacc
     6481 aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttgatc
     6541 tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa
     6601 taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg ggagaattac
     6661 gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag cagtttttcg
     6721 atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag atattctgtt
     6781 gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg
     6841 tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact
     6901 accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa gtcaacaatc
     6961 ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc
     7021 cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat accgtactct
     7081 tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt
     7141 acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca
     7201 tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc
     7261 cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt
     7321 ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg tagcggcgac
     7381 cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg cccgcgacgt
     7441 tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc agcacccgct
     7501 cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc ataacagcat
     7561 tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca
     7621 gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga
     7681 catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg
     7741 agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc attgcaactt
     7801 cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc
     7861 gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat
     7921 gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg
     7981 gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc
     8041 gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt tctacctgct
     8101 tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag acatattcca
     8161 tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact
     8221 gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg
     8281 caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt
     8341 ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc cgctccacgc
     8401 ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct atttttggcg
     8461 aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg
     8521 tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc gcccctttca
     8581 ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg acttcgccgg
     8641 ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc tcatcttgcg
     8701 gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg cctttcgctt
     8761 ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca tccggctctt
     8821 gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc aaaaacagat
     8881 tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc aggttttcca
     8941 tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct cctttgatcg
     9001 agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc ggctgcaaaa
     9061 ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa aatgtctgat
     9121 aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg cattgtggga
     9181 acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc attctggctt
     9241 tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa caggctgatg
     9301 cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc cgccatgccg
     9361 acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg gcgggatgca
     9421 ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct ggcgtaggga
     9481 aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag cacaggcgcg
     9541 atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt tctaaacatc
     9601 gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag atcgattttg
     9661 agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc gagatcaccc
     9721 cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat gttcggctgc
     9781 ttattcactt ccccctggct ttgggtgtaa tcatcaccac cgccgggaat tgggctttca
     9841 ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg gaagtactcc
     9901 gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac cagaaaaaag
     9961 gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg tgctgccccg
    10021 tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt cttcatgctt
    10081 cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct tccagttcaa
    10141 taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg gcgataggcg
    10201 gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc attttgctgg
    10261 tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg taagccaata
    10321 aaataccgag gaaagtcccc accatcgcat gtgcgataag cgcccccagc tcggcggcag
    10381 gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca ataccaaacg
    10441 ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc tcgctttcgt
    10501 gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg tgaccgctga
    10561 taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg aggatgcgtg
    10621 ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc agcgaaaaca
    10681 tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga tccatataca
    10741 ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc gtgcctttaa
    10801 tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca ataatcacca
    10861 gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa ccgccgaaaa
    10921 ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt
    10981 gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa aaaaaagcag
    11041 cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa acagcctgta
    11101 ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa agtttacgtc
    11161 tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca acaggctggt
    11221 gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc agtaatccac
    11281 tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc cagcagtggt ccttcttctg
    11341 cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg acgccattac
    11401 acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca tgaacgtttt
    11461 gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc ggtgggcttc
    11521 cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt tcgctttcca
    11581 gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata tcccgcgctt
    11641 cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg cttcttcagg
    11701 ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa tttgctggag
    11761 atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt caaaacggaa
    11821 gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaag
//
LOCUS       DAJTNP010000109        11776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTNP010000109 DAJTNP010000000
VERSION     DAJTNP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            11..487
                     /locus_tag="OO160_003330"
     CDS             11..487
                     /locus_tag="OO160_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5525333.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            550..771
                     /locus_tag="OO160_003331"
     CDS             550..771
                     /locus_tag="OO160_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5525334.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            934..1308
                     /locus_tag="OO160_003332"
     CDS             934..1308
                     /locus_tag="OO160_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5525335.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1355..1729
                     /locus_tag="OO160_003333"
     CDS             1355..1729
                     /locus_tag="OO160_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5525336.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1726..2217
                     /locus_tag="OO160_003334"
     CDS             1726..2217
                     /locus_tag="OO160_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525337.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2229..2426
                     /locus_tag="OO160_003335"
     CDS             2229..2426
                     /locus_tag="OO160_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525338.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2511..3071
                     /locus_tag="OO160_003336"
                     /pseudo
     CDS             2511..3071
                     /locus_tag="OO160_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3143..4147
                     /locus_tag="OO160_003337"
     CDS             3143..4147
                     /locus_tag="OO160_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5525339.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            4429..4710
                     /locus_tag="OO160_003338"
                     /pseudo
     CDS             4429..4710
                     /locus_tag="OO160_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(4860..4947)
                     /locus_tag="OO160_003339"
     tRNA            complement(4860..4947)
                     /locus_tag="OO160_003339"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4911..4913),aa:Ser,seq:gga)
     gene            5182..6120
                     /gene="ghrA"
                     /locus_tag="OO160_003340"
                     /pseudo
     CDS             5182..6120
                     /gene="ghrA"
                     /locus_tag="OO160_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6175..6912
                     /gene="ycdX"
                     /locus_tag="OO160_003341"
     CDS             6175..6912
                     /gene="ycdX"
                     /locus_tag="OO160_003341"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5525340.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            6936..7490
                     /gene="ycdY"
                     /locus_tag="OO160_003342"
     CDS             6936..7490
                     /gene="ycdY"
                     /locus_tag="OO160_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5525341.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            7592..8083
                     /locus_tag="OO160_003343"
     CDS             7592..8083
                     /locus_tag="OO160_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5525342.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(8216..8922)
                     /locus_tag="OO160_003344"
                     /pseudo
     CDS             complement(8216..8922)
                     /locus_tag="OO160_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(8949..9365)
                     /gene="csgF"
                     /locus_tag="OO160_003345"
     CDS             complement(8949..9365)
                     /gene="csgF"
                     /locus_tag="OO160_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5525343.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9390..9779)
                     /gene="csgE"
                     /locus_tag="OO160_003346"
     CDS             complement(9390..9779)
                     /gene="csgE"
                     /locus_tag="OO160_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5525344.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(9784..10434)
                     /gene="csgD"
                     /locus_tag="OO160_003347"
     CDS             complement(9784..10434)
                     /gene="csgD"
                     /locus_tag="OO160_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5525345.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            11187..11642
                     /gene="csgB"
                     /locus_tag="OO160_003348"
     CDS             11187..11642
                     /gene="csgB"
                     /locus_tag="OO160_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5525346.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            11683..>11776
                     /locus_tag="OO160_003349"
     CDS             11683..>11776
                     /locus_tag="OO160_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5525347.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGN"
BASE COUNT         3103 a         2759 c         2849 g         3065 t
ORIGIN      
        1 ggcagggaca atgcagcagc tttcctttct gcccggaaac atgacgcccg gcgagcgcag
       61 tctcattctg cgggccctgc aaaccctgga ccgccatctt catgaacccg gcgtggcctt
      121 cacctccacc cgtgcggcac gggaatggct gattctgaac atggcgggac tggagcggga
      181 agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
      241 caccggcacc atcaaccgta cggaagttca tccccgggaa gtgattaaac gcgccctgta
      301 ccacaatgcc gctgccgtgg tgctggcgca caatcacccg tccggtgaag tcacacccag
      361 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
      421 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
      481 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcagagg
      541 tatcacatca tgagaatcat cacccgtggt gaagccatgc gtattcacca acagcatcct
      601 gcatcccgtc tttttccgtt ctgtaccggt aagtatcgct ggcacggcag tgctgaagcg
      661 tatactggtc gtgaagtgca ggatattccc ggcgttcttg ccgtgtttgc agaacgccgt
      721 aaggacagtt ttggcccgta tgtccggctg atgagtgtca ccctgaactg atgttccgtc
      781 acccgggaaa cgagtacgac cagatgacct cagacgccgc tgtggatgag gccattctca
      841 tcaatgcata catcttcacc gaaaccggtc agcgcgccgg ctgacctgtc atcgttaaac
      901 cattcttaac cacttttcag agaggatttt atcgtgtcag acaaactctc cgggataact
      961 catcccgatg acaatcacga ccgcccctgg tgggggcttc cctgcacagt gaggccctgt
     1021 tttggcgctc gtctggtgca agagggtaac cgcctgtatt accttgccga ccgcgccggt
     1081 atcagaggcc gcttcagcga cgcagatgca taccatctgg accaggcctt tccgctgctg
     1141 atgaaacaac tggaactcat gctcaccagc ggtgaactga atccccgata tcagcatacc
     1201 gtcacgctgt atgcaaaagg gctgacctgc gaagccgaca ccctcggctc ctgtggttac
     1261 gtttatctgg ctgtttatcc gacaccagca cccgcaacca cctcataacc ccgaagctct
     1321 cacctttagc actaccagtt acagagaact taagatgaaa acattatccg acacacatgt
     1381 acgggaggta tctcgctgcc cgtctcccgt caccatctgg cagacactgc tcatccgact
     1441 gctggaccag cattatggcc tcacactgaa tgacacaccg tttgtcgatg aacgtgtgat
     1501 tgagcagcat attgaggcag gcatttcact gtgtgatgca gttaattttc tcgtggaaaa
     1561 atacgcgctg gtgcgtaccg accagccggg attcagcacc tgtccccgct ctcagttaat
     1621 aaacagtatt gatatcctcc gggctcgccg ggcgaccggc ctgatgaccc gcgacaatta
     1681 cagaacggta aataacatta ccctgggtaa gtatccggag gcgaaatgat gaaactggcc
     1741 ctgacactgg aagccgacag cgttaacgta caggcactga acatagggcg cattgtcgtt
     1801 gacgtcgatg gtattgagct cgctgaactg attaacatgg tctgcgataa cggctactcc
     1861 cttcgtgttg ttgatgaatc tgaccagacc tcagcagaat gcacgccacc atttgctacc
     1921 cttaccggca tacgctgcag taccgcacat atcacggaaa cggacaacgc ctggttgtac
     1981 tcgctgtcac accagaccag tgacttcggt gaatcagaat ggattcattt tacaggtagc
     2041 ggatatctgt tacgtaccga tgcgtggtca tacccggttc tgcggcttaa acgcctgggg
     2101 ctgtcaaaaa cgttccgtcg tctggtcgtc acactcatcc ggcgttatgg cgtcagtctc
     2161 attcatctgg atgccagcgc tgaatgcctg ccgggtttac ccactttcga ctggtaacca
     2221 ggaacaacat gaaatcatta accacggaaa cagcgctgga tattctgatt gcgtggctgc
     2281 aggacaatat cgactgcgga tcgggaatta tctttgacaa cgatgaggat aaaacggatt
     2341 cagcagcact gttgccctgt atcgaacagg cccgggagga tgtccgtacc ctgcgtcatc
     2401 tgcagcttct gcaccagaac cggtgaagct cactcaccag acttcattcc tctgacgcca
     2461 gcctgcacag ggctggcgtt ttcatttatc tgcaaaaagg aatatcgatt atgtcagata
     2521 tcacaatctc ccgtccggaa gtggtcaacg ggcatacgga cgttatctgc tcaaccagca
     2581 ttcgccacat tctggctgta cgaaagagta cactgttgca aatcgacaca cttatccgac
     2641 aactggctga aatctcggtg ctgacggaaa gcatcggagg taaaactgcc ccggactggg
     2701 ccatgaaaca ggattttcgc tgcggctgct ggctgatgga gaaacccgaa acagcaatga
     2761 aagccattac ccggaatctt gatcgcgaaa tctggcgtga cctgatgcaa cgttccggga
     2821 tgctgtcatt aatggatacg caggcccgtg atacatggta ccggtcactg gagtacgata
     2881 attttccgga aatcagcgaa gcgaacattc tgagcacatt tgaacaactg caccagaata
     2941 aggatgaggt gtttgagcga ggggtgatca acgtcttcag agggctgaac tggaattaca
     3001 aaaccaattg cccctgtaaa tttggcagta aaattatcgt caacaatctg gtgagatggg
     3061 accggtgggg ataatgatat ggtcaccccc accttaacag cagtacgctg ttaaggtaaa
     3121 tccctttaac ggagagacca tcatgcagaa catcacactt attggtatcg atctcggaaa
     3181 gcactccttc cacattcatt gtcaagacaa gtctggtaag gctcttctgc gtaaaaaatt
     3241 tacccgtact aaactgatgc agtttctggc gacatgtccg ccctcagttg ttgtaatgga
     3301 agcctgtgcg ggtgcacact ttatggctcg ctgtatctct gattttgggc atgaaactaa
     3361 gctgatttct ccacagttcg ttcgcccgtt tgtcaaaagt aataagaatg attttgttga
     3421 tgcagaagcc atatgcgaag cggcttcacg accatcaatg cgttttgtcc agccaagaac
     3481 tgagacccag caggccatga gggcgttaca ccgggtcaga gaatcgctca tcagagataa
     3541 ggtgaaaaca accaaccaga tccacggttt cttgctggaa tttggaatca gcctgccgat
     3601 aggtgatgct gtaataaaac ggttgtctct cgtgttggct gaacacgaaa tccccgaata
     3661 tctgagccgc cttttggtga ggttgcatac ccactacttt taccttgttg agcaaatagc
     3721 agagctggaa tcagaattaa gtcagtcaat caacgctgat gataccgctc agcgcattat
     3781 gacaattcct ggtgttggtc cgattactgc cagcttacta tcgtcgcaac ttggtgacgg
     3841 gaaacagttc tcatgcagca gggattttgc agcttccaca ggacttgttc cccggcagta
     3901 tagcactgga ggaaaatcga ctctgttggg tatcagtaag cgaggtgata aaaatttacg
     3961 ccggttactg gttcagtgtg cccgctcatt cataatgcga cttgaacgca agcatggaaa
     4021 acttgctgag tgggttcggg agcaactcaa caagaagcat tcgaatgtgg ttacctgcgc
     4081 tctggcaaat aaactggctc gaattgcctg ggcaatcact actcagaaaa aagaatatca
     4141 ggcctgatat agatgctgat ataactcaat tagtttattt taaacaataa gatacaagtt
     4201 acctttctgg ttttgcgaag actgattatt gatgacatga acggcaaatc gacctgatta
     4261 aaaacctaac agatgaaatg gctcactgaa gccgagggga tttttaggat tatcaggtgc
     4321 ggaactcatc ggggcgcggg catccttgcc cacagagacg ccggatagat ttaagcaagc
     4381 caaccattaa tcaaaaatca gtgttgcaaa aacgggggtg accatagatt tcatcttatc
     4441 accggacaac aggctgaccg actcgccgat ctggaaagaa tgttgcacct gttcagcggt
     4501 aaaccaatcc ccgacaaccg gggaaacatc acccttcgtc tggatgatca cattcagtct
     4561 gttcagggta aagagagcta tgaagatgag atgttcagca tcagatactt taagaagggc
     4621 tccgcgcata tcacgttcag aaaaccagaa ctggttgaca gactgaatga tattatcgca
     4681 aaacactatc ctgatatgct ggcagtatag ctttgccagt atgctcaaag atcggaaatt
     4741 gtccgataag atatgatcgc ggggcatata aaccggtttc agatggcata cccgatggca
     4801 tactgtctgg aaggaaaaat aaaaaagcgc atgttaaaca ataacatgca cttataatct
     4861 ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg
     4921 ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc tgtcttgctg gaacgggcgc
     4981 taatttaggg aaatcatgac ctgaggtcaa caaacttttt gaaaaaatcg cgcgtttatt
     5041 caaacattca atcaatgtgt ggttttaata agcgaaatct gctttttttg ccaccgacca
     5101 cggatttgtt atgctggtgg cctttgtaga tcataacgat aagtgcgaat aaatttcgca
     5161 caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt cgatacccaa
     5221 tggtggattg aggcactgcg caaagctatt cctcaggcaa gagtcagggc atggaaaagc
     5281 ggagataatg actctgctga ttatgtttta gtctggcatc ctcctgttga aatgctggca
     5341 gggcgcgatc ttaaagcggt gttcgcactc ggggccggtg ttgattctat tttgagcaag
     5401 ctacaggcac accctgaaat gctgaaccct tctgttccac tttttcgcct ggaagatacc
     5461 ggtatgggcg agcaaatgca ggaatatgct gtcagtcagg tactgcattg gtttcgacgt
     5521 tttgacgatt atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc tgaatatcat
     5581 cgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggctgtaa agttgctcag
     5641 agtctgcaaa cctagcgctt tccgctgcgt tgctggagtc gaacccgtaa atcgtggcct
     5701 ggcgtgcaaa gctttgccgg acgggaagaa ctgtctgcat ttctgagcca atgtcgggta
     5761 ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca acaattactc
     5821 gaaaaattac ctgatggcgc gtatctcctc aacctggcgc gtggtgttca tgttgtggaa
     5881 gatgacctgc tcgcggcgct ggatagcggg aaagttaaag gcgcaatgct ggatgttttt
     5941 aatcgtgaac ccttaccgcc tgaaagtccg ctctggcaac atccacgcgt gacgataaca
     6001 ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg agtacatttc tcgcactatt
     6061 gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg tggctactaa
     6121 taaagcatca ggattcctgc tatccttggc gggaattgaa tacaggagag agttatgtat
     6181 cccgtcgacc ttcatatgca taccgttgcc agcacacatg catatagcac attgagtgat
     6241 tacattgccc aggccaaaca aaagggcatt aaactttttg cgatcaccga tcatggcccg
     6301 gatatggaag atgcgccgca tcactggcac ttcattaaca tgcgtatctg gccgcgagtg
     6361 gttgatgggg tagggatcct gcgcggcatt gaagctaaca ttaaaaatgt tgatggtgaa
     6421 attgactgca gcggtaaaat gtttgactcg ctggatctga ttattgccgg ttttcatgag
     6481 ccggtttttg cgccacatga caaagcgacc aatacacaag cgatgatctc cactatcgcc
     6541 agcggcaatg tgcatataat aagccatccc ggaaatccga aatatgaaat agatgttaaa
     6601 gccgttgctg aagcagccgc gaaacatcag gtggcgctgg aaatcaataa ttcctcattt
     6661 ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagctgcagc ggtacgtgat
     6721 gctggaggtt gggtggcatt aggctcggat tctcacaccg cgtttaccat gggggaattt
     6781 gaagagtgtc ttaaaatcct cgacgcggta gattttccgc tagagcgcat tttgaatgtt
     6841 tctccgcgcc gcttactgaa cttccttgaa tctcgcggta tggcaccgat tgcggaattt
     6901 gcagaccttt aattactagt aatggaaata tattaatgaa cgagttttct atcctctgtc
     6961 gtgtgctggg ttcgctctat taccgccaac cgcaagatcc tttactggtg ccgctgttta
     7021 ctctgattcg tgaggggaaa ctggctgcga actggccact ggagcaggat gagttactga
     7081 cgcgtttaca gaaaagctgt gatatgacgc aagtctctgc cgattacaat gcgttgttta
     7141 tcggcgatga atgtgccgtg ccgccatatc gtagcgcatg ggttgaggac gcaacggaag
     7201 cggaagtgcg cgcttttctt tccgaacgag ggatgctact ggcggatacg ccagccgatc
     7261 acatcggcac attgctgctt gcggcttcct ggctggaaga tcagtcaacg gaagatgaga
     7321 gcgaagcact ggaaacactg ttcagtgagt atctgttacc ctggtgtggt gcgttcctcg
     7381 gcaaagtgga ggcccatgca accacgcctt tctggcgcac catggcaccg ctaacccgtg
     7441 atgccattag tgcaatgtgg gacgagctgg aagaagattc tgaagagtaa atgtgatcat
     7501 cgtcactcat aactgcaaca gataatttgt tgttgcataa aatgtgtgct cgatctcatt
     7561 catggccgcg ttttctgcta tcatgcgcgg catgaacata cttctctcta ttgcaatcac
     7621 aacaggcatt ctctccggta tctggggatg ggtggctgtt tccctgggct tactaagctg
     7681 ggcgggcttc ctgggctgta cggcctactt tgcttgcccg caaggtgggc tgaaagggct
     7741 ggcgatctcc gctgcaaccc tgcttagtgg cgtggtgtgg gcgatggtca ttatttacgg
     7801 tagtgcactg gcaccacatc tggaaattct cggttatgtc ataaccggta ttgtcgcgtt
     7861 tctgatgtgt attcaggcca aacagctgct gctttcattt gttccgggga cgtttatagg
     7921 cgcatgcgcg acgtttgccg ggcagggtga ctggaaactg gtgttacctt cgctggcgct
     7981 tgggctggta tttggttacg caatgaaaaa cagcggcctg tggctggcgg cgcgtagtgc
     8041 aaagacggct caccgtgagc aggaaatcaa aaataaagcg tgaggggcac tcacgctttc
     8101 gcttaaacag taaatgccgg attattaatt ccggcatttt tatctgtcag gattccggtg
     8161 gaaccgacat atggcggtat ttcaccagaa tgtcattctg ccgttctgct ttattttaca
     8221 aatcccacag accacggtcg ataccatcat taatcaggaa aatgacccct gtttcgatag
     8281 ccgacatcag acacagcata acgggttcgt ttgaggtgta acccacttcc ccttccagca
     8341 agcgctggta gtcaataaag cggaaaaccc cggcctgaac ttcataggaa agtatcgtct
     8401 tactggtgtt caccgaggag aggatctcac cggtactcac attgacgaca cgcaggttca
     8461 cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat cccaaaatat cttgccccaa
     8521 caccgcccga tttaacgttg ctttcataac cgataatcga accttcaacc ataatatttg
     8581 ccgccgttaa agattgcagc gggattcggt tattaatggc aaccgtgccg ttttcttgtg
     8641 ccgcacgaat aaccttgcgc tcgttaagca ggttttgtaa gccctggcgc tttgcggaac
     8701 agcagtggag aagttacttg ccgggtaggg tttaaattgc ccggtttcgt cctgaatgtt
     8761 gtataccgaa acaaagatct tacccgtcgg cgctggcaga tgggtcaaat ctttgtagct
     8821 ctgagcacga ggcattaatg tcggtctggc ggcttcttta ggcggggcgg ttaagcatcc
     8881 gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc atgattattt tccttatgaa
     8941 gctggggctt aaaaatcggt tgagttattt tgtaaacccg aaacctggat ggtcgaggtt
     9001 tgtccggttt tacgatctgt cacgttcaac tgcaattgac catcgcggtt agcaatatca
     9061 acaatataat cgttggtcac catgcggccc ggtttaccgg tattaatatt cgacagtagc
     9121 ccacctaaaa tttgtgactg gatggcctga gtaaagttat ctaacgctga gggtgtttca
     9181 ataccaaagt catcgttata gctcggatct ttataagagt tttgggcctg agcgctattt
     9241 aataaaaaag cgccattatt tgggttacca ccaaagtttg gattacggaa ctggaaagtc
     9301 atggttccag cccaacttaa tggcgaaata agcatgagta gaactactgc atgtttgaca
     9361 cgcattgcag cctccgaaca attttttatt tagaattcat catgcgccaa atcgtccgta
     9421 cttaataacg cctgatttat ctggcgacga tttagtgctt cttcagtttg aatcagtgca
     9481 aagacgacag ttttctcgga gtctcttttc aacgggaata aaaaagtctg gaaaataaca
     9541 tcctgattga ccgttattgt gatccagctt ccccatcgtg cactgggcct ttcattaatc
     9601 gttaagttac ccgtatagtc actttcccat ttatcactaa aggctcggta aaaatcatgg
     9661 ccaatagatg aaacagtatg gtcagttaac aatcccggga cttctacctc aacggcgtga
     9721 agattccctg cggcgaacag aaattctgcc gccacaatcc agcgtaaata acgtttcatg
     9781 gctttatcgc ctgaggttat cgtttgccca ggaaaccgct tgtgtccggt tttttacggc
     9841 tatcttcttg aaaagattat aaagatgcgt tttaaccgta ttttcgctga tgaacaacga
     9901 acgagcgatc tcgttattag acgcgccgat acgcagctta ttcaggatct ctttttcccg
     9961 atgagtaagg agggctgatt ccgtgctgtt ataacgatag ttaccagaat gtgtaatcag
    10021 gtaactggca agcttttgcg taaagtagca ttcgccacgc aggacgcctt gcaacccatt
    10081 gacaacacgt tcttgatcct ccatggcata aaaaacgccg ttgatatgag gccagttttc
    10141 aatgtcgcgg tacgggtaat cttcaggcgt atttagcaac aatattttga tattgttgtt
    10201 tttcctgctc aaagtatcct gccaataatg gataagcttt ttatccgctt ccatcatatc
    10261 cagaagaata atagagcctg aagagatatc gtccagagaa cgttgaatat tatgtaattt
    10321 tcctgtaatt gctagcgatt gtttaaggtg ctgcaagaga gctgtcgcct gcaaagaagg
    10381 tttagtgatc aacaataatg tatgaccatg aatactatgg acttcattaa acatgatgaa
    10441 accccacttt tttattgatc gcacacctga cagctgcctc taaaatagaa gcaccagaag
    10501 tactgacaga tgttgcactg ctgtgtgtag taataaatca gccctaaatg ggtaaaatat
    10561 aaaactaatg gattacatct gatttcaatc tagccattac aaatcttaaa tcaagtgtta
    10621 aacatgtaac aaaatgtaac tcattatatt aaaatgttaa cattaaggtt ttattaagtt
    10681 tagaaatgat agaaaagttg tacatttggt ttttattgca caattttaaa aaatcataca
    10741 aatggtgcta acttactaat aatacatata aaaaatattt cggtgtagtc ctttcgtcat
    10801 gtaaaacgtt tttgtttttt ctccacacct ccgtggacaa ttttttactg caaaaagacg
    10861 aggtttgtca cggcttgtgc gcaagacata tcgcagcaat cagcgacggg caagaagaat
    10921 gactgtctgg tgatttttga tagcggaaaa cggagattta agagaaaaca aaatattttt
    10981 tgcgtagata acagcgtatt tacgcgggtt ttaatacttt ggtatgaaca aaaaaagaaa
    11041 aatacaacgc gcgggtgagt tattaaaaat atttccgcag acatactttc catcgtaacg
    11101 cagcgttaac aaaatacagg ttgcgttaac aaccaagttg aaatgattta atttcttaaa
    11161 tgtacgacca ggtccagggt gacaacatga aaaacaaatt gttatttatg atgttaacaa
    11221 tactgggtgc gcctgggatt gcagccgcag caggttatga tttagctaat tcagaatata
    11281 acttcgcggt aaatgaattg agtaagtctt catttaatca ggcagccata attggtcagg
    11341 ctgggactaa taatagtgct cagttacggc agggaggctc aaaacttttg gcggttgttg
    11401 cgcaagaagg tagtagcaat cgggcaaaga ttgaccagac aggagattat aaccttgcat
    11461 atattgatca ggcgggcagt gccaacgatg ccagtatttc gcaaggtgct tatggtaata
    11521 ctgcgatgat tatccagaaa ggttctggta ataaagcaaa tattacacag tatggtactc
    11581 aaaaaacggc agttgtagtg cagagacagt cgcaaatggc aattcgcgtg acacaacgtt
    11641 aattaccatt cgacttttaa atcaatccga tgggggtttt acatgaaact tttaaaagta
    11701 gcagcaattg cagcaatcgt attctccggt agcgctctgg caggtgttgt tcctcagtac
    11761 ggcggcggcg gtaacc
//
LOCUS       DAJTNP010000110        11716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTNP010000110 DAJTNP010000000
VERSION     DAJTNP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            128..997
                     /locus_tag="OO160_003350"
     CDS             128..997
                     /locus_tag="OO160_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5525348.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="OO160_003351"
     CDS             complement(1200..1553)
                     /locus_tag="OO160_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5525349.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO160_003352"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO160_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5525350.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="OO160_003353"
     CDS             complement(3381..3674)
                     /locus_tag="OO160_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5525351.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="OO160_003354"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="OO160_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5525352.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO160_003355"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO160_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5525353.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="OO160_003356"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="OO160_003356"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5525354.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="OO160_003357"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="OO160_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5525355.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="OO160_003358"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="OO160_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5525356.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="OO160_003359"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="OO160_003359"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5525357.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="OO160_003360"
     CDS             11054..11629
                     /locus_tag="OO160_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5525358.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2814 a         3024 c         2881 g         2997 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcatccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaagcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtac agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg acgctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcctt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gagctcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccacgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgag cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct agcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggcc gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcaaacgcat ctcaacttta cacaaatcaa
    10681 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgctgccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agatta
//
LOCUS       DAJTNP010000111        11674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTNP010000111 DAJTNP010000000
VERSION     DAJTNP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..338
                     /locus_tag="OO160_003361"
     CDS             <1..338
                     /locus_tag="OO160_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5525359.1"
                     /translation="MCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQL
                     RKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLL
                     LRATGGVCV"
     gene            complement(455..730)
                     /gene="yjfN"
                     /locus_tag="OO160_003362"
     CDS             complement(455..730)
                     /gene="yjfN"
                     /locus_tag="OO160_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5525360.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(879..1208)
                     /gene="bsmA"
                     /locus_tag="OO160_003363"
     CDS             complement(879..1208)
                     /gene="bsmA"
                     /locus_tag="OO160_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5525361.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1390..2139
                     /gene="yjfP"
                     /locus_tag="OO160_003364"
     CDS             1390..2139
                     /gene="yjfP"
                     /locus_tag="OO160_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5525362.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2136..2891)
                     /gene="ulaR"
                     /locus_tag="OO160_003365"
     CDS             complement(2136..2891)
                     /gene="ulaR"
                     /locus_tag="OO160_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5525363.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2999..4063)
                     /gene="ulaG"
                     /locus_tag="OO160_003366"
     CDS             complement(2999..4063)
                     /gene="ulaG"
                     /locus_tag="OO160_003366"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5525364.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4418..5815
                     /gene="ulaA"
                     /locus_tag="OO160_003367"
     CDS             4418..5815
                     /gene="ulaA"
                     /locus_tag="OO160_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5525365.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5831..6136
                     /gene="ulaB"
                     /locus_tag="OO160_003368"
     CDS             5831..6136
                     /gene="ulaB"
                     /locus_tag="OO160_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5525366.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6146..6610
                     /gene="ulaC"
                     /locus_tag="OO160_003369"
     CDS             6146..6610
                     /gene="ulaC"
                     /locus_tag="OO160_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5525367.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6624..7274
                     /gene="ulaD"
                     /locus_tag="OO160_003370"
     CDS             6624..7274
                     /gene="ulaD"
                     /locus_tag="OO160_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5525368.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7284..8138
                     /gene="ulaE"
                     /locus_tag="OO160_003371"
     CDS             7284..8138
                     /gene="ulaE"
                     /locus_tag="OO160_003371"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5525369.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8138..8824
                     /gene="ulaF"
                     /locus_tag="OO160_003372"
     CDS             8138..8824
                     /gene="ulaF"
                     /locus_tag="OO160_003372"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5525370.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8953..9228)
                     /locus_tag="OO160_003373"
     CDS             complement(8953..9228)
                     /locus_tag="OO160_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5525371.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9555..9950
                     /gene="rpsF"
                     /locus_tag="OO160_003374"
     CDS             9555..9950
                     /gene="rpsF"
                     /locus_tag="OO160_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5525372.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9957..10271
                     /gene="priB"
                     /locus_tag="OO160_003375"
     CDS             9957..10271
                     /gene="priB"
                     /locus_tag="OO160_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5525373.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10276..10503
                     /gene="rpsR"
                     /locus_tag="OO160_003376"
     CDS             10276..10503
                     /gene="rpsR"
                     /locus_tag="OO160_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5525374.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10545..10994
                     /gene="rplI"
                     /locus_tag="OO160_003377"
     CDS             10545..10994
                     /gene="rplI"
                     /locus_tag="OO160_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5525375.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11065..11628)
                     /locus_tag="OO160_003378"
                     /pseudo
     CDS             complement(11065..11628)
                     /locus_tag="OO160_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2764 a         2870 c         3085 g         2955 t
ORIGIN      
        1 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
       61 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
      121 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
      181 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
      241 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
      301 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
      361 tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg cgctcgcgcc
      421 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
      481 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
      541 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
      601 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
      661 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
      721 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
      781 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
      841 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
      901 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
      961 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     1021 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     1081 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     1141 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     1201 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     1261 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     1321 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     1381 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     1441 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     1501 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     1561 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     1621 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     1681 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     1741 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     1801 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     1861 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     1921 acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     1981 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     2041 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     2101 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     2161 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     2221 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     2281 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     2341 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     2401 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     2461 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     2521 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     2581 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     2641 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     2701 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     2761 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     2821 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     2881 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     2941 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     3001 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     3061 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     3121 agataaacgg cttgaagcca tacttcagac gatctttttt gatctcccac agcacgcgga
     3181 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     3241 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     3301 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     3361 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     3421 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     3481 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     3541 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     3601 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcatcagcac
     3661 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     3721 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     3781 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     3841 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     3901 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     3961 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     4021 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     4081 atagttcaat tcgaatcaac atgtgattgg ttttgattaa tcctgacact attccttcag
     4141 gaaggtaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgggg
     4201 aaagatttaa tggaatgtgt agttaaatta actaaatgaa tttaaataaa taattgtttc
     4261 gttgtgtgaa tcccactcta tccatgtgga attatttgcg ggtcgcgtca catttaatca
     4321 taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca ttccgggtgt
     4381 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     4441 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     4501 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     4561 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     4621 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     4681 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     4741 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     4801 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     4861 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     4921 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     4981 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     5041 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     5101 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     5161 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     5221 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     5281 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     5341 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     5401 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     5461 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     5521 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     5581 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     5641 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     5701 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     5761 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     5821 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     5881 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     5941 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     6001 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     6061 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     6121 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     6181 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     6241 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     6301 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     6361 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     6421 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggcggtcg atgccaacac
     6481 gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     6541 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
     6601 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
     6661 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
     6721 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
     6781 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
     6841 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
     6901 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
     6961 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
     7021 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata
     7081 tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg
     7141 cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc gccggtcgca
     7201 gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg
     7261 aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc atctatgaaa
     7321 aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa acgttgggct
     7381 tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg cctgtcgcgc ctcgactgga
     7441 gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga aaccggcgtg cgtgtgccgt
     7501 ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg
     7561 cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg
     7621 tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa acgcgtcgtc
     7681 gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcgcag gtgacgctgg
     7741 cgatggagat catggattat ccgttgatga actccatcag caaggcgctg ggttacgcgc
     7801 actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg tcggcgtggg
     7861 acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg gtgcatgtga
     7921 aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg gtggatttcg
     7981 aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg attgagatgt
     8041 ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgtgtgat tgggtgaaag
     8101 cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctaa aacagcaggt
     8161 gtttgaagcc aacatggatc tgccgcgcta tgggctggtg acctttactt ggggcaacgt
     8221 cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag cccagcggcg ttgcctacga
     8281 aaccatgaaa gcagacgata tggtggtggt cgatatgagc ggcaaggtgg tggaagggga
     8341 gtatcgccca tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt acccgtcgct
     8401 tggcggcatt gtccataccc actccactca tgccaccgca tgggcgcagg cggggctggc
     8461 gatcccggca ttaggcacca cgcacgctga ctacttcttt ggtgatatcc cgtgtacgcg
     8521 tggattaagc gaagaagagg tgcagggtga gtatgaactg aacactggca aagtgattat
     8581 cgaaacgctg ggcaacgccg agccgctgca tacgccggga attgtggtgt atcagcacgg
     8641 accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtag tgatggaaga
     8701 agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactcaatc atatcgacag
     8761 ctacctgatg aataaacact tcatgcgtaa gcacggtcct aacgcttatt acgggcagaa
     8821 gtaaaacacg cgctgcggaa atttccttcc tcgggagata actggtctaa ttccgcagcc
     8881 gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg
     8941 ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg
     9001 ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc gcatctgttt cttcatttaa
     9061 tgcctgacgt gcttcgcttt cagtggcgaa attatgattg atataaatca cgcctaagct
     9121 ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg cccatgtatt
     9181 tgcacttagc aaaagcacag ccagaagggc taaaactcga ctgaacatag atacctcctc
     9241 gacggctgac tttgtgtgct ctcctctgtg atgatcttct gatttaattt taatcaatga
     9301 taaagaagtt gatggtgacc atttctgatg cagttgttca aaaaaacact gtgacggaat
     9361 gtgatgaact tcaaatcagc ggtttagagg ttatttgcga aaggggagat ttatttctgc
     9421 tccgcccttg agtttagcgg ggtatgccag tactataacg cgtcattttt cagccgacct
     9481 ttaacacgtt ccttgcctcc ccgggattcg gctgacccag acaggaggct gaataatccg
     9541 taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat ggtccatcct gaccagagcg
     9601 aacaggttcc gggcatgatc gagcgctaca ctgctgccat cactggtgca gaaggcaaga
     9661 tccaccgtct ggaagactgg ggccgccgtc agctggctta cccgatcaac aaactgcaca
     9721 aagcacacta cgttctgatg aacgttgaag ctccgcagga agtgatcgat gagctggaaa
     9781 ctaccttccg cttcaacgat gccgttatcc gcagcatggt tatgcgtacc aagcacgctg
     9841 ttaccgaagc atctccgatg gttaaagcga aagacgagcg ccgtgagcgt cgcgatgatt
     9901 tcgcaaacga aaccgctgat gatgctgaag ctggggattc tgaagagtaa tttctgatga
     9961 ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc ccttcgaaag gtcagtccat
    10021 caggaattcc tcactgccag ttcgtgcttg agcatcgttc tgtgcaggag gaagccggct
    10081 ttcaccggca ggcatggtgt caaatgcccg ttattgttag cggacacgaa aaccaggcca
    10141 ttactcacag tataacggtc ggcagtcgca taaccgttca ggggttcatt tcatgccaca
    10201 aggcaaagaa cggactgagc aaaatggttt tgcatgccga gcagattgaa ttgatagatt
    10261 ctggagacta gccatatggc acgttatttc cgtcgtcgca agttctgccg tttcaccgcg
    10321 gaaggcgttc aagagatcga ctataaagat atcgctacgc tgaaaaacta catcaccgaa
    10381 agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg caaaatacca gcgtcagctg
    10441 gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc cgtacactga tcgccatcag
    10501 taatcggtca cggtccatta atacgacttt gagaggataa ggtaatgcaa gttattctgc
    10561 ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt aaacgttaaa gcgggctatg
    10621 ctcgtaactt cctggtaccg cagggtaaag ctgttccagc taccaagaaa aacattgaat
    10681 tcttcgaagc acgtcgcgct gaactggaag ctaaactggc tgaagttctg gcagctgcta
    10741 atgctcgcgc tgagaaaatc aatgcactgg aaactgttac catcgcgtct aaagctggcg
    10801 acgaaggtaa actgttcggt tccatcggta ctcgcgacat cgctgacgct gtaactgcag
    10861 ctggcgttga agtggctaag agcgaagttc gtctgccgaa cggcgttctg cgtaccactg
    10921 gcgaacacga agtgagcttc caggttcaca gcgaagtatt cgcgaaagtg atcgtaaacg
    10981 tagtagctga ataattcgtt attcaacgag acgtaaaaac gccgaccatt ggtcggcgtt
    11041 ttgctttcta tttttcgtca ggcattaatt tcgcaagtag atccattcac cattctcttg
    11101 tgtccataag tcaggatggt ttgctggcgt tagatgcata cgatcactcc ttatgtctcg
    11161 gtcctgcggc actcgctcga aacctaaacc cgagtagatt gcggctgaat ttgataagga
    11221 acgtagacca attttcaaat catggggtaa atcagaatac gcgtttagca atgcagcaat
    11281 gatgaatccg agggcattag aaacgcctgt aacagtgtga agttggtcga tataaagatc
    11341 gattgttggt agttcagatg agcagcaatt aggcatttgg gcaggaaggg ttacaacgta
    11401 tcctatcgtt tggccggtat agtctggagt actataacca ggtcccaaaa caacctcgtg
    11461 tattgtgtat ttactgttgg gttcgaagga ttcgacatct tgcaataaat atttcagttc
    11521 tgcggttaac ctcgcacccg cgtaagcttt attgtatttc tgggcgtgaa tattatctaa
    11581 tgtagcttca tggttccatc caaaagaatt atcaccattc attgttactg aatcgccacg
    11641 ggtttaacag acacctcaga gtcatttaag atgg
//
LOCUS       DAJTNP010000112        11374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTNP010000112 DAJTNP010000000
VERSION     DAJTNP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            317..1957
                     /gene="trg"
                     /locus_tag="OO160_003379"
     CDS             317..1957
                     /gene="trg"
                     /locus_tag="OO160_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5525376.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1995..2918)
                     /locus_tag="OO160_003380"
     CDS             complement(1995..2918)
                     /locus_tag="OO160_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5525377.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3135..4478
                     /locus_tag="OO160_003381"
     CDS             3135..4478
                     /locus_tag="OO160_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5525378.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4703..6358
                     /gene="opgD"
                     /locus_tag="OO160_003382"
                     /gene_synonym="mdoD"
     CDS             4703..6358
                     /gene="opgD"
                     /locus_tag="OO160_003382"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5525379.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6498..6722
                     /locus_tag="OO160_003383"
     CDS             6498..6722
                     /locus_tag="OO160_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5525380.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6785..7321
                     /gene="rimL"
                     /locus_tag="OO160_003384"
     CDS             6785..7321
                     /gene="rimL"
                     /locus_tag="OO160_003384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5525381.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7316..8296)
                     /locus_tag="OO160_003385"
     CDS             complement(7316..8296)
                     /locus_tag="OO160_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525382.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8420..9412
                     /gene="tehA"
                     /locus_tag="OO160_003386"
     CDS             8420..9412
                     /gene="tehA"
                     /locus_tag="OO160_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5525383.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9409..10002
                     /gene="tehB"
                     /locus_tag="OO160_003387"
     CDS             9409..10002
                     /gene="tehB"
                     /locus_tag="OO160_003387"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5525384.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10305..10961
                     /locus_tag="OO160_003388"
     CDS             10305..10961
                     /locus_tag="OO160_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5525385.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10979..11374)
                     /gene="tnpA"
                     /locus_tag="OO160_003389"
                     /pseudo
     CDS             complement(10979..11374)
                     /gene="tnpA"
                     /locus_tag="OO160_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2933 a         2661 c         2857 g         2923 t
ORIGIN      
        1 atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc
       61 acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg
      121 tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct
      181 tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg
      241 tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg
      301 acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat
      361 caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag
      421 ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc
      481 ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat
      541 caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa
      601 tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa
      661 tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc aacgctttca
      721 ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc
      781 gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt
      841 gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca
      901 gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac
      961 gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca
     1021 acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa
     1081 tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt
     1141 agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg
     1201 caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc
     1261 cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag
     1321 caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt
     1381 agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt
     1441 catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc
     1501 gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc
     1561 aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt
     1621 aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga
     1681 tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag
     1741 tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa
     1801 tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg
     1861 attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg
     1921 cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca
     1981 gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa
     2041 ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt
     2101 cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa
     2161 gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga agtagacagc
     2221 gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc
     2281 tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc
     2341 gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga
     2401 aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg
     2461 atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact
     2521 tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc
     2581 agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga
     2641 tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat
     2701 tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc
     2761 gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt
     2821 tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc
     2881 aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg
     2941 gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaaca
     3001 attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca
     3061 gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac
     3121 tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc
     3181 aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg
     3241 tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa
     3301 atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg
     3361 cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct
     3421 attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg
     3481 atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta
     3541 tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca
     3601 ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac
     3661 aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag ttagcaacgg
     3721 tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct
     3781 gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc
     3841 agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc
     3901 gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt
     3961 ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg
     4021 tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg
     4081 ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca
     4141 gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg
     4201 gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg
     4261 gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga
     4321 tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg
     4381 aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag
     4441 cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca
     4501 aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg
     4561 atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag
     4621 cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca
     4681 ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt caatggctat
     4741 ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc
     4801 agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat
     4861 ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct
     4921 ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg
     4981 gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg
     5041 tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt
     5101 tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca
     5161 aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga
     5221 tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg
     5281 tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga
     5341 ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc
     5401 gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag
     5461 tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg
     5521 catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac
     5581 aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg
     5641 gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa
     5701 cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat
     5761 gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg
     5821 caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt
     5881 ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta
     5941 ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg
     6001 catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg
     6061 tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga
     6121 gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc
     6181 catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt
     6241 cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca
     6301 gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc
     6361 gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca
     6421 tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc
     6481 actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg
     6541 aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca
     6601 gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa
     6661 agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt
     6721 aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga
     6781 taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag
     6841 tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct
     6901 aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt
     6961 gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat
     7021 cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg
     7081 gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga cattgattca
     7141 tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc
     7201 gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc
     7261 tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata
     7321 ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac
     7381 ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat
     7441 ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt
     7501 cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta
     7561 acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg
     7621 gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat
     7681 cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg
     7741 cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga
     7801 caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg
     7861 gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata
     7921 ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac
     7981 cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca
     8041 gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag
     8101 cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc
     8161 agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta
     8221 ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta
     8281 ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg
     8341 cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt
     8401 aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt
     8461 tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg
     8521 gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt
     8581 attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt
     8641 tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc
     8701 gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt
     8761 ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga
     8821 agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat
     8881 ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt
     8941 ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc
     9001 cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc
     9061 ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg
     9121 actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa
     9181 tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct
     9241 ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac
     9301 caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt
     9361 gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt
     9421 gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga agtactggaa
     9481 gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac
     9541 agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt
     9601 atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc
     9661 gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg
     9721 ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact
     9781 aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc
     9841 gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag
     9901 agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt
     9961 attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg
    10021 cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt
    10081 atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga
    10141 aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa
    10201 ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt
    10261 tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat
    10321 ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca
    10381 cccagccaga taaatataac aattactctg atttaaaaga aacaacctca gcaacaggta
    10441 aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac agcatcgtct
    10501 ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg cagaaagttc
    10561 tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg cagcgtaaac
    10621 cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt accggtgtag
    10681 ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta gtggttgcgg
    10741 ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt gaaggtgagc
    10801 tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc gaaggtaaag
    10861 acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt attgatgaca
    10921 tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg cgccatcgtt
    10981 agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg ggatataagg
    11041 cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg atgacggata
    11101 ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg cctttgtagt
    11161 aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat acgcctttga
    11221 ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac aaatggacgt
    11281 gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat acatttgaaa
    11341 agtaagtgcg tagtttttct gtcgcgtcat ggtc
//
LOCUS       DAJTNP010000113        11314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTNP010000113 DAJTNP010000000
VERSION     DAJTNP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(223..298)
                     /locus_tag="OO160_003390"
     tRNA            complement(223..298)
                     /locus_tag="OO160_003390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Ile,seq:cat)
     gene            857..3109
                     /locus_tag="OO160_003391"
     CDS             857..3109
                     /locus_tag="OO160_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525386.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3445..4377
                     /gene="csiD"
                     /locus_tag="OO160_003392"
     CDS             3445..4377
                     /gene="csiD"
                     /locus_tag="OO160_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5525387.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4397..5665
                     /gene="lhgO"
                     /locus_tag="OO160_003393"
     CDS             4397..5665
                     /gene="lhgO"
                     /locus_tag="OO160_003393"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5525388.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5688..7136
                     /gene="gabD"
                     /locus_tag="OO160_003394"
                     /pseudo
     CDS             5688..7136
                     /gene="gabD"
                     /locus_tag="OO160_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7144..8430
                     /gene="gabT"
                     /locus_tag="OO160_003395"
                     /pseudo
     CDS             7144..8430
                     /gene="gabT"
                     /locus_tag="OO160_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8668..10068
                     /gene="gabP"
                     /locus_tag="OO160_003396"
     CDS             8668..10068
                     /gene="gabP"
                     /locus_tag="OO160_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5525389.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10089..10751
                     /gene="csiR"
                     /locus_tag="OO160_003397"
     CDS             10089..10751
                     /gene="csiR"
                     /locus_tag="OO160_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5525390.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10752..11201)
                     /gene="kbp"
                     /locus_tag="OO160_003398"
     CDS             complement(10752..11201)
                     /gene="kbp"
                     /locus_tag="OO160_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5525391.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
BASE COUNT         2895 a         2783 c         2831 g         2805 t
ORIGIN      
        1 gatcaatttg cccgcttggc atagcgggca ttttttccag gtacttttga atgagtactg
       61 atgaataaat acattgcagt ggcgtgccac gtaccaaaac accagccctc attcgaaacc
      121 acccaccgca cttcttcctt gaaatggcgt tagtcatgaa atatagaccg ccatcgagta
      181 ccccttgtac ccttaactct tcctgataca tgaatgatga tttggtggcc cttgctggac
      241 ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa ccactgagct aaagggcctt
      301 aagtatgcaa taacaatact tataaaccac gcaataaaca tgatgatctt atgatgtaaa
      361 taacagattt ttatgcgttc ccattcacct tgggtcgtga ttacacgcga cataaaaccc
      421 gacaccgcct ccattcgcaa agtcgatact cgcagtcaac aagcaaatgt taataattag
      481 cactatctat agttatcatc gattcaatga tactttgtaa tgattttgta tctaataata
      541 taactttatt acattagctg aagagttttc gcatcattat gatatctgtt acttttcact
      601 ccataaaaat aaacttcata tagcaatata ttctttcata gatcttattc tgctaatcat
      661 tagtttcgta tgcgcgattt ttgacagttg cctctccaga ccacatcgat aattaaaaaa
      721 cagatttaaa cattatcctt ttccatatag atattggata aaaataggac atctgtttgc
      781 aattactttc acaacaatta aacattttta tgttttcgta tacatcatat tactctacca
      841 ttagaggaac gttattatgt tttctataaa accaggacct ggaaatttac ctatcgacaa
      901 ccccacattg ttatcatgga acattactga tggggatcta aattccaaat ttaatacatt
      961 agaatatcta aactgtataa caaatattat taatgcttgt ggagtttatc ctcaggattt
     1021 aaaagacaga gaaattatat caacttttca cgcagaaaaa gtcattaatg atctgttaaa
     1081 aaacgattat aaaatttccc tttctccaga tacaacttac cgagagctga ataaagctgc
     1141 acagagtagc attacagcgc cagacaggat aggagaaggg aaaacatggg tttatcaacg
     1201 agatacaatg gttgaaagag gtgataacag tggtgttcat cagtatggtc cagctgaaca
     1261 tttcactcac attatatctg acaaaccgtc cccaaaagat aagtatgttg catatgctat
     1321 taacattcct gactatgagc tagcagccga tgtatataat attaacgtga cgtcaccttc
     1381 cggacagcaa gaaacattta aaatactaat caatccagaa catctacggc aaacacttga
     1441 gcgtaaatct cttactgctg ttcagaaatc acaatgtgaa atcatcaccc ccaaaaaacc
     1501 tggcgaagcg attcttcatg cttttaatgc cacctaccag caaatcaggg aaaatatgtc
     1561 tgagtttgca cgttcccatt atgggtatat acaaatccct ccagtgacaa ctttccgcgc
     1621 cgacggacca gaaactcccg aagaagaaaa aggttactgg tttcacgctt atcaacccga
     1681 agatctttgt accatccata atccaatggg ggatttgcag gattttatcg cattggttaa
     1741 agatgctaaa aaatttggta tcgatatcat tcctgattat acctttaact ttatgggaat
     1801 cgggggaagt ggtaaaaatg acctggatta tccctctgct gatatacgag cgaagatcag
     1861 taaagatata gaaagtggta tccctggcta ttggcaaggt caggttttga ttccattcat
     1921 taaagatcca gtaacaaaag aacgtaaaca aatccatcca gaagatatac atctcactgc
     1981 aaaagacttc gaagcaagta aagataacat ctctaaggat gaatggaaaa acctccatgc
     2041 attaaaagaa aagagtttaa atggaatgcc taaaacaacc cccaaaagtg accaggttat
     2101 tatgttgcaa aatcaatacg ttcgtgaaat gcgaaaatat ggcgtacgag gtttacgtta
     2161 tgatgcagca aaacactcaa aacatgaaca aatagaaaga tcaataaccc caccgcttaa
     2221 aaattataat gagcgattac acaatactaa cttatttaac ccaaaatatc atgaaaaaac
     2281 cgttatgaat tacatggaat atctggtaac ttgtcagttg aatgaagaac aaatgtcatc
     2341 gctgctttat gaaagagatg atttaagcgc cattgatttt tcattgctca tgaagacgat
     2401 aaaagccttt tcattcgggg gagatctcct aacccttgca tcaaaaccgg gttccacaat
     2461 ctcaagcatc ccatcagaaa gacggatatt gattaacatt aaccacgatt ttcctaacaa
     2521 tggcaatctt ttcaatgact ttctatttaa ccatcaacaa gatgaacaat tagcaatggc
     2581 atatatggcc gctctcccgt tcagcaggcc tttagtttac tgggatggcc aggtattaaa
     2641 atcaacgact gaaattaaaa attatgatgg gcccacgcgt gtcggtggtg aggcgtggct
     2701 taataaaggt tgctctacct atcagcagct ctacaatgaa ttccacgcat tatatataga
     2761 taaagcagga atatggagcg catttgaggg tgtatttgca actaaaaacg ttctggcctt
     2821 tagtcgtggg gattctgtga acattaatca ctctcctcat gatggactag ttataataaa
     2881 taaaggaaac gaagaagttg aaggtacctg gcctaacaaa ttgcaacctg gaatatataa
     2941 aaacatgggg agtaatagcg ttaacattat tattaataat acccgaaaaa ttatcccccc
     3001 ttgtaaagta tttacgctta gaggcggatc tctaaatatc aatattccta ggcgtagcgc
     3061 tcttctttta gggaaaacag gagaaccgcc gaactatctc tatttataat ttaatgttat
     3121 atctgccccg ataaaacggg gcagataata tgtttagttt actaacggtc attttgtagt
     3181 gaagccattt actgtttttt atcgaccaga taatctgttc tctaatgtta actcccccta
     3241 acctgttgct ttagttattc atttcctgtc tcactttgcc ttaataccct acgttaaatg
     3301 ttactaattt gttgcttttg atcacaataa gaaagcaata tgtcgctttt gtgcgcattt
     3361 ttcagaaatg tagatatttt tagattatgg ctacgaaatg agcatcgcca tgtcacccta
     3421 catctcataa gaggatcgct tctgatgaat gcactgaccg ccgtacaaaa taacgctgtc
     3481 gattcaggcc aggactatag cggattcact ctcatcccgt cggcgcaatc cccgcgtctg
     3541 ctggaactca ccttcaccga acagacgacc aaacagtttc tcgaacaggt tgccgaatgg
     3601 cccgtgcagg cgctggagta caaatcgttt ctgcgttttc gggtaggcaa aattcttgac
     3661 gatctgtgtg cgaatcagct gcaaccattg ctgttgaaga ccctgctaaa ccgtgctgaa
     3721 ggtgcgctgt tgatcaatgc ggtaggtatc gatgatgtgg cgcaggcaga tgagatggtg
     3781 aagctggcaa cggcggtagc gcatctgatt ggtcgctcaa atttcgatgc gatgagcggt
     3841 cagtattacg cgcgtttcgt ggtgaaaaat gtcgataact cagacagcta tctgcgtcag
     3901 ccgcaccgcg tgatggaact gcacaacgac ggcacgtatg tcgaagagat caccgattac
     3961 gtgctgatga tgaaaatcga tgactgggaa catctggacc actatttccg ccatccactg
     4021 gcgcgtcgcc cgatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa agatgttttc
     4081 catccagtgt tcgacgttga tcaacagggt cgcccggtga tgcgctatat cgaccagttc
     4141 gtccagccaa aagattttga agaaggcgtg tggttgagcg agctttccga tgccattgaa
     4201 accagcaaag gcattctttc tgtacccgtt cccgttggca aattcctgtt gataaacaac
     4261 ctgttctggc tgcacggtcg cgaccgcttt actccgcacc cggatctgcg ccgtgaactg
     4321 atgcgtcagc gtggctattt cgcttacgcc actcaccact accagacgca tcagtaagcg
     4381 caaaggaatt gagcggatgt atgattttgt gattattggc ggcggcatca tcggcatgtc
     4441 gaccgccatg caactgattg atgtttatcc ggatgcccgc attgcgttgc tggaaaaaga
     4501 gtccggcccg gcctgtcacc agacgggcca caacagcggc gtgatccatg ccggggtcta
     4561 ttacacgccc ggcagcctga aggcgcagtt ttgcctggcg gggaaccgtg ccaccaaagc
     4621 cttttgcgat caaaacggca ttcgctatga caactgcggc aagatgctgg tcgccacgtc
     4681 tcagctcgaa atggaacgga tgcgcgcctt atgggaacgc acagcggcga acggtatcga
     4741 gcgcgagtgg ataaacgccg tggaactgcg cgagcgcgaa ccgaatatca ccgggcttgg
     4801 tggcattttt gtgccgtcca gcggcattgt cagctaccgc gaagtgacgg cggcaatggc
     4861 aaaaatcttc caggccagag gcggcgagat tatttataac gccgaagtca gcgccctcag
     4921 tgagcataaa aacggcgtgg tgatacgcac ccgtcagggc ggtgaatatg aagcatcgac
     4981 gctgattagc tgttccgggc tgatggctga ccggctagtg aaaatgctcg gcctcgaacc
     5041 gggctttatt atctgcccgt tccgtggcga gtatttccgc cttgcgccgg agcataacca
     5101 gattgttaac cacctgattt accccattcc cgaccccgcg atgccatttt tgggcgtgca
     5161 tctcacccgc atgatcgacg gcagcgtgac cgtcgggcca aacgcggtgc tggctttcaa
     5221 acgcgaaggc tatcgcaagc gcgacttctc atttagtgac acgctggaga ttttgagctc
     5281 gtcggggatt cgccgggtgc tgcaaaacca tctacgctca ggactgggcg agatgaaaaa
     5341 ctcgctgtgc aaaagcggct atctgcggct ggtgcaaaag tattgtcccc ggctttcgtt
     5401 aagcgatctc cagccctggc ccgccggtgt gcgggcgcag gcagtatcgc cggacggcaa
     5461 gctgattgac gattttctgt ttgtcaccac cccacgcacg atccacacct gcaatgcgcc
     5521 ctccccggca gcgacatcag caattcctat tggtgcgcat attgtcagca aggtacaaac
     5581 gctgttggca agccagagta accccggacg cacgctgcga gcggcacgta gtgtggatgc
     5641 cttacacgcc gcatttaatc aataaccttt gaaaacagga cgtaacaatg aaacttaacg
     5701 acagtaactt attccgccag caggcgttga ttaacgggga atggctggac gccaacaatg
     5761 gtgaagtcat cgacgtcacc aatccggcga acggcgacaa gctgggtagc gtgccgaaaa
     5821 tgggcgcaga tgaaacccgc gccgctatcg acgccgccaa ccgcgccctg cccgcctggc
     5881 gtgcgctcac cgccaaagaa cgcgccacca ttctgcgcaa ctggttcaat ttgatgatgc
     5941 agcatcagga cgatttagcg cgcctgatga ccctcgaaca gggtaaaccg ctggctgaag
     6001 cgaaaggcga aatcagctac gccgcctcct tcattgagtg gtttgctgaa gaaggcaaac
     6061 gtatttatgg cgacaccatt cctggtcatc aggccgataa gcgcctgatt gttatcaagc
     6121 agccgattgg cgtcaccgcc gccatcacgc cgtggaactt tccggcggcg atgattaccc
     6181 gtaaagccgg tccggcgctg gcggcaggct gcaccatggt gctgaagccc gccagtcaga
     6241 cgccgttctc tgcgctggcg ctggcggaac tggcgattcg cgcgggcatt ccggctggcg
     6301 tgtttaacgt ggtcaccggt tcggcaggcg cggtcggtaa tgaactgacc agcaacccgc
     6361 tggtacgcaa actgtcgttt accggttaga ccgagattgg ccgccagtta atggaacagt
     6421 gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg caacgcgccg tttatcgtct
     6481 ttgacgatgc cgatctcgat aaagccgtgg aaggcgcgct ggcctcgaaa ttccgcaacg
     6541 ccgggcaaac ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg tatgaccgct
     6601 ttgccgaaaa attacagcag gcggggagca aactgcacat cggcgacggg ctggagaaag
     6661 gcgtcaccat cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa gagcatattg
     6721 ccgatgcgct ggagaaaggt gcgcgtgtgg tttgcggcgg taaagcgcac gaacgcggcg
     6781 gcaacttctt ccagccaacc attctggtgg acgttccggc caacgccaaa gtgtcgaaag
     6841 aagagacgtt cggccccctc gccccactgt tccgttttaa agatgaagcc gatgtgattg
     6901 cacaagccaa tgacaccgag tttggtcttg ccgcctattt ctacgcccgt gatttaagcc
     6961 gcgtcttccg cgtcggcgag gcgctggagt acggcatcgt cggcatcaat accggcatta
     7021 tttccaatga agtggcccca ttcggcggca tcaaagcctc gggtctgggt cgtgaaggtt
     7081 cgaagtatgg catcgaagat tacttagaaa tcaaatatat gtgcatcggt ctttaactgg
     7141 agaatgcgaa ggaacagcaa taaagagtta atgcagcgcc gcagtcaggc gattccccgt
     7201 ggcgttgggc aaattcaccc gattttcgct gaccgcgcgg aaaactgccg ggtgtgggac
     7261 gtttaaggcc gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa taccgggcac
     7321 ctgcatccga aggtggtggc cgcggtggaa gcgcagttga aaaaactgtc gcacacctgc
     7381 ttccaggtgc tggcttacga gccgtatctg gagctgtgcg agattatgaa tcagaaggtg
     7441 ccgggcgatt tcgccaagaa aacgctgctg gttacgaccg gttccgaagc ggtggaaaac
     7501 gcggtaaaaa tcgcccgcgc cgccaccaaa cgtagcggca ccatcgcttt tagcggcgag
     7561 tatcacgggc gcacgcatta cacgctggcg ctgaccggca aggtgaatcc gtactctgcg
     7621 ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt atccttgccc gctgcacggc
     7681 ataagcgagg atgacgctat cgccagcatc caccggatct tcaaaaatga tgccgcgccg
     7741 gaagatatcg ccgccatcat gattgagccg gttcagggcg aaggcggttt ctacgccgcg
     7801 tcgccagcct ttatgcagcg tttacgcgct ctgtgtgacg agcacgggat catgctgatt
     7861 gccgatgaag tgcagagcgg cgcggggcgt accggcacgc tgtttgcgat ggagcagatg
     7921 ggcgtggcgc cggatcttac cacctttgcg aaatcgatcg caggcggttt cccgctggcg
     7981 ggcgtcaccg ggcgcgcgga agtgatggac gctgttgcac cgggcggtct gggcggcacc
     8041 tatgccggta acccgattgc ctgcgtggct gcgctggaag tgttgaaggt gttcgagcag
     8101 gaaaatctgc tgcaaaaagc caacgatctg gggcagaagc taaaagacgg attgttggcg
     8161 atagccgaaa aacacccgga gatcggcgac gtacgcgggc tgggatcgat gattgccatc
     8221 gagctgtttg aagatggcga tcacagcaaa ccggacgcaa aactcaccgc cgagatcgtg
     8281 gcacgcgccc gcgataaggg cctgattctt ctctcctgcg gcccgtatta caacgtgctg
     8341 cgcatccttg taccactcac cattgaagac gctcagatcc gtcagggtct ggagattatc
     8401 ggtcagtgtt ttgatgaggc gaagcagtag caccgtcccc atgcaggagg cgacgctgcg
     8461 cgtcttgtcc ggcctacgag aatccaagtc ggataaggcg tttacgccgc atccgacaat
     8521 ctgtacgtga acaggaataa atctatgtcg gccggataag gcggagccgc tctccggcaa
     8581 aaagaatcaa taacaattaa acgcgtgacc cgacgcggga aatgtcgggg cgctctccca
     8641 agtgacacac tttcgagagg attcaggatg gggcaatcat cgcaaccaca cgagttaggc
     8701 ggcgggctga aatcacgcca cgtcaccatg ttgtctattg ccggtgttat cggcgcaagt
     8761 ctgtttgtcg gttccagcgt cgccatcgcc gaagcggacc cggcggtatt actggcctat
     8821 ctgttcgccg gattactggt ggttatgatt atgcggatgc tggcggaaat ggcagttgcc
     8881 acgcccgata ccggttcgtt ttccacctat gccgataaag ccattggccg ctgggcgggc
     8941 tataccatcg gctggttgta ctggtggttt tgggtactgg ttatcccact ggaagccaac
     9001 atcgccgcca tgatcctgca ctcgtgggtt ccaggcattc ccatctggtt attttccctc
     9061 gtcattaccc tcgccttaac tggcagtaat ttattaagcg ttaaaaacta cggcgaattt
     9121 gagttctggc tggcgctgtg caaagtcatc gctatcctgg cctttatttt ccttggtgca
     9181 gtcgcaatta gcggttttta cccttatgcc gaagtgagcg ggatctcaag attgtgggat
     9241 agcggcggct ttatgcccaa cggtttcggt gcggtattaa gcgcgatgtt gatcaccatg
     9301 ttctcgttta tgggcgcaga aattgtcacc attgccgccg cggaatccga catgccggaa
     9361 aaacatattg tccgcgccac caactcggtt atctggcgta tttctatctt ctatttgtgc
     9421 tctatttttg tcgtcgtggc attaataccg tggaatatgc cggggctgaa agccgttgga
     9481 tcttatcgct cggttctgga attgctcaat attccccatg cgaaattaat catggactgt
     9541 gtgatattac tttccgtaac cagttgcctg aactcggccc tgtataccgc ctcaaggatg
     9601 ctctactcct taagccgtcg cggtgatgct cccgcggtaa tgggtaaaat caaccgcagt
     9661 aaaaccccgt acgtggcggt gttactctcc accggcgcag cattcttaac ggtggtggtg
     9721 aactattacg cgcctgcgaa ggtatttaaa tttctgatcg acagctccgg cgctatcgcc
     9781 ctgctggttt atttagtcat cgccgtttca cagttgcgga tgcgcaaaat tctgcgagca
     9841 gaaggaagcg aaattcgctt gcgaatgtgg cttgatccct ggctcacctg gctggtcatc
     9901 ggctttatta cctttgtgtt ggtagtgatg ctattccgtc cggcgcaaca gttagaagtg
     9961 atctccaccg gcttattagc gatagggatt atctgtaccg tgccaattat ggctcgctgg
    10021 aaaaagctgg tattgtggca aaaaacaccc gttcataata cgcgctgata atgatcagga
    10081 gtcacaccat gaccattacg tctctggatg gctatcgctg gctgaagaac gatattattc
    10141 gcggtaattt tcaaccggat gaaaaattac ggatgagttt actgacatcg cgttatgcac
    10201 ttggcgttgg gccgttgcag gaagcacttt ctcaactggt ggcggaacgg ctggtcacgg
    10261 tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga gcaggagctg ctcgatattt
    10321 tcgacgcccg cgccaatatg gaagcgatgc tggtcagtct ggcgattgcc cgcggtggcg
    10381 atgaatggga ggcagacgtt ctcgcaaaag cgcatctgct cagtaagctg gaagcctgtg
    10441 acgccagcga gaaaatgctt gatgagtggg atctgcgtca tcaggcgttt catacagcaa
    10501 ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg tgaacggttg tttgatctgg
    10561 cggcgcgtta tcgatttatc tggctgcggc gaacggtgct ttcggtggaa atgctggagg
    10621 ataaacacga tcagcaccag accctgactg cggcggtact gccgcgagat acagcgcgcg
    10681 ccagtgagtt aatgcgccag catttactga cgccaattcc cattatccag caggcgatgg
    10741 ctggcaatta actactcttc cggaatacgc aacacttgcc ccggataaat tttatccggg
    10801 ctttttagca tcggtttatt cgcttcgaag attttattgt acagattagc gttaccgtag
    10861 acctgtttgg aaatggcact cagagtgtcg ccagacttaa cggtataaaa ctggctggca
    10921 gtggctggtg tcgccgtttt cacctgatca tcaacactgg caataccgga aatattcccc
    10981 accgcaacaa ggattttctc cttcgcctcc tgactcaggc cgtcaccagt gaccgtcgct
    11041 ttgccgtcgg caatttgaat gttcacttta tcggcatcag gtataccggt tttgctcaga
    11101 tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct gacctgtaac cgcatcccag
    11161 agtttttctc cggcatcttt aacaaaattg aacagaccca tagctacctc attcgttaac
    11221 ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt aacgccgcgg gggtgagcac
    11281 aggtttaatc gcgggtttgc acccagaacg cgtg
//
LOCUS       DAJTNP010000114        11259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTNP010000114 DAJTNP010000000
VERSION     DAJTNP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO160_003399"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO160_003399"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5525392.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO160_003400"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO160_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5525393.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="OO160_003401"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="OO160_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5525394.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="OO160_003402"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="OO160_003402"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5525395.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="OO160_003403"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="OO160_003403"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5525396.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="OO160_003404"
     CDS             complement(5841..6881)
                     /locus_tag="OO160_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5525397.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="OO160_003405"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="OO160_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5525398.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="OO160_003406"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="OO160_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5525399.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="OO160_003407"
     CDS             7823..8263
                     /locus_tag="OO160_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5525400.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="OO160_003408"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="OO160_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5525401.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="OO160_003409"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="OO160_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5525402.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11259
                     /gene="rsxC"
                     /locus_tag="OO160_003410"
     CDS             9492..>11259
                     /gene="rsxC"
                     /locus_tag="OO160_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5525403.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2623 a         2899 c         2838 g         2899 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgctcca aagcgcgcaa gctggaacag caacaggcta
    11221 atgcggaacc agaagaacag gtcgatccgc gcaaagccg
//
LOCUS       DAJTNP010000115        11111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTNP010000115 DAJTNP010000000
VERSION     DAJTNP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO160_003411"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO160_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5525404.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO160_003412"
     tRNA            complement(621..695)
                     /locus_tag="OO160_003412"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO160_003413"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO160_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO160_003414"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO160_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5525405.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO160_003415"
     CDS             complement(2932..3990)
                     /locus_tag="OO160_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525406.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO160_003416"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO160_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5525407.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO160_003417"
     CDS             6068..6352
                     /locus_tag="OO160_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5525408.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO160_003418"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO160_003418"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5525409.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO160_003419"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO160_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5525410.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO160_003420"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO160_003420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5525411.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2545 c         2748 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt c
//
LOCUS       DAJTNP010000116        11044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTNP010000116 DAJTNP010000000
VERSION     DAJTNP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            407..1237
                     /locus_tag="OO160_003421"
     CDS             407..1237
                     /locus_tag="OO160_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5525412.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1378..2151)
                     /gene="uxuR"
                     /locus_tag="OO160_003422"
     CDS             complement(1378..2151)
                     /gene="uxuR"
                     /locus_tag="OO160_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5525413.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2366..3826)
                     /locus_tag="OO160_003423"
     CDS             complement(2366..3826)
                     /locus_tag="OO160_003423"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5525414.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3907..5091)
                     /gene="uxuA"
                     /locus_tag="OO160_003424"
     CDS             complement(3907..5091)
                     /gene="uxuA"
                     /locus_tag="OO160_003424"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5525415.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5431..6774
                     /gene="gntP"
                     /locus_tag="OO160_003425"
     CDS             5431..6774
                     /gene="gntP"
                     /locus_tag="OO160_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5525416.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7025..7927)
                     /gene="fimH"
                     /locus_tag="OO160_003426"
     CDS             complement(7025..7927)
                     /gene="fimH"
                     /locus_tag="OO160_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5525417.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7947..8450)
                     /gene="fimG"
                     /locus_tag="OO160_003427"
     CDS             complement(7947..8450)
                     /gene="fimG"
                     /locus_tag="OO160_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5525418.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8463..8993)
                     /gene="fimF"
                     /locus_tag="OO160_003428"
     CDS             complement(8463..8993)
                     /gene="fimF"
                     /locus_tag="OO160_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5525419.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9003..>11044)
                     /gene="fimD"
                     /locus_tag="OO160_003429"
     CDS             complement(9003..>11044)
                     /gene="fimD"
                     /locus_tag="OO160_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5525420.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2683 a         2828 c         2682 g         2851 t
ORIGIN      
        1 gtagatgaaa atacttgtca ccgctgctac ttaattccgg tgggaaatta tttatttccg
       61 cataatcagc tatgtattat ttgaaacaat tagtttgtaa ataatataaa ttgataacat
      121 atcaaataca tcaaaaatac cattcacagg cattattaat atagacctca atatagcaat
      181 atcaaattcc aattagtaat aagatatgct tttaaattgc atgcaaataa ttttaaatga
      241 gcgttttttg atactttaga gtgtttccta ccctttaata tcactgtgta gagttgatat
      301 cactacaaca gaaacatgca tgtcatttgt ttacttctct ttttactaca gaaaaattta
      361 tctatttttc taaacatcag ttaaatactc ataaaaggta actattatgt ctatgccatt
      421 aagcaatgcc gctaataacc aattcaatac aaataatcac ttccttcatc atccaaaagt
      481 cgacagtgag ttaacacgtc gatatgaata tgcacgcctg gatacggaaa atatctatct
      541 tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg tcgaaattag
      601 aaaacttaat attggcaaac agtcctggcc atttaattat gtgacagata catgcagaga
      661 attcaatggt attacaacaa caggacgtat gccctatcga aacttgaaaa tcacctcagc
      721 tttagacgaa atatatggtg gtatatgtaa aaaggcgcat gctgctacag agttagcaga
      781 aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg actatacagt
      841 tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa ccacgatagg
      901 atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc agccaagaat
      961 tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt ccggattcgc
     1021 ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca ttggtataga
     1081 agcctatcct ggtaccgcgg agatccattc aaaaatggga tataacgtta tccccggtga
     1141 tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag aactgtttga
     1201 attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa tgagtactaa
     1261 tattcaggtt atacagatat ttaaacaaaa ccctgcaatt tacaaagtaa tactcattgc
     1321 gacttcttaa tttaagaaag cgcaaggaac gtttaccctt gcgcttatta taataagtca
     1381 ggcgtcgact ttatccagcg gccatgaatc aaagagatag ccgtcaaaat aaatatcgtc
     1441 aacgttcgag aattccagca gacgttgctt aacgttttcc agatgctgcc acattgccag
     1501 cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt gatcgcccaa
     1561 ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc acattggatt
     1621 gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt tatgcgttgc
     1681 ttcagcaatg gcgagatgga actgcatgtc accgctttcg ctgctacccg gcgcgctgga
     1741 agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat cttcgcgggt
     1801 agcctgcagt gcggcaaact cggcgatgtt actttccagt aactgacgcg cctgtaacag
     1861 ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt tgtggctgcc
     1921 tgagctatca agcacataga taccggcacc ccggcgcact tccaccagcc ctttgatctc
     1981 cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt ctgcaatttc
     2041 acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca gatcgtggat
     2101 catcgccccg acttcctggt aaggtctttg cgcagaggtg acagatttca tggtgttgtg
     2161 tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct tatccggcct
     2221 acaacgggca attttacggc ccccgttaat ctcaaactat accattagca ctggaagttg
     2281 gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt tgattgacca
     2341 gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc gcaccgcgtt
     2401 cgcacagctg ctgatatgcc gacgtcaccg cgccaacaaa gtcggcattc tgcggcagat
     2461 catcggcaaa aataccgctc aggcccagca gcgctttcac gcggtctgcg ccctgatact
     2521 gcgcgttaat cttctggaac tcggccagca tcgggtcgac cacgtcaatg gcattaccct
     2581 gctcatccac gccctgggtg taacccatcc agccagccac gcccagcgcc aggtgacgcc
     2641 agctgccgcc gttttgcagg tgcagacgca ccgggtccag tagacgctgc ggtaacttct
     2701 ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag agacgggttg ctgaaacgct
     2761 cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac agcgttggcg
     2821 cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccgggtta gtcatggtgt
     2881 cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag tggctgccgt
     2941 tcagcatacg cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac tgtgtgccca
     3001 ctttatccca gtccgggcga ccattaacga agttatcttc aatcacccac tgacggaacg
     3061 gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt tcctgtaagg
     3121 tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag gtgacgttct
     3181 cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc cagccccagt accgcgacct
     3241 tcgccacatg gccgttttca cgcacgttat cgcaggacat caccgtaaat gctttcagcc
     3301 ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac ttcggcgcag
     3361 tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagttga ccgcttgccg
     3421 catcagcgca gtagcctttt tccgtgaccg ttagagagac aatcgccgtt tgcggacgcg
     3481 ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg atgcagcgct tctttcatcg
     3541 aaccgataat tttcagctcg gtgctttctg cgcctttttc cgctacggtg tacagcagtt
     3601 gctgtttttt caggttttcg atcagcatgc ggtcgttgcc tggcatcagg ttaacttcgc
     3661 agatgcccca gtcgctgtcg gtgctttcca gcagatgatg ggtatacagc gcctggtgcg
     3721 cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga gaatgatccc
     3781 atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta aactccagaa
     3841 tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca gccatgccgg
     3901 tgggtattaa cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc gaacttcggc
     3961 caggcctttc agacgaccaa ttgcggagta accagggttg gttttcttct tcaggtcgtc
     4021 cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc cttccgcttt
     4081 acgacggtgt tcttcttcaa caatcgcttt caccactttg tacatatcaa cgtcaccgtt
     4141 caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg agcgcagatg
     4201 ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt tgtcagcacg
     4261 cacgccgtag gaaccggtgc acatggtgaa accgttcgcc atgctgttta cggtatcaac
     4321 catccactgc atatcttcaa tggtggaaac aatgcgcggc aagccgagga tcgggcgcgg
     4381 cggatcgtcc gggtgaaccg ccatacgcac gccaacttct tcagcaactg gaataatcgc
     4441 tttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt tgtacagctc
     4501 caggtgctta cggaactggt cgagggtata cccttcttcc gcgcccggca gaccggcaat
     4561 gatgttacgc gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc gttcagctgc
     4621 ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct ggacgtttca ggatatgcat
     4681 ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt ctggcagcac
     4741 gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc acacggtgcg
     4801 aatgccacac tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc actgctcata
     4861 gttgccagtg tgggttttga tatcttcgtg aattggtacg ctttctacga cagaccacac
     4921 caggcctgcg tcttcaacga tcgccttgcg tttgaggatc tcttctacgg accatacttc
     4981 gccgttcggg atatggtgca gcgcggtaac cacgccagtt gcgcccgcct gacggacatc
     5041 agctaaagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca tgatttttcc
     5101 tctttcacat agtgggttcc acagcaaaga gacgcggaac cttggagttg tttaggtatc
     5161 cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg tataagttct
     5221 accttttggt aagacatgtc aaccagaatt ctgaaattgg ttaaccgcgt cgcaagaatt
     5281 tcactctcat ggttgttgct gcaaattgca gcaaagggta atcgatggtt attgatcaga
     5341 ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg cccaaaataa
     5401 caacggttca accaagtaag gtaaaacgtt atgcatgtgc ttaacattct ctgggtggta
     5461 ttcggcattg gcctgatgct gatactgaat ttgaagttca aaatcaattc aatggtggct
     5521 ttgttggtgg cggcgctgtc cgtcgggatg ctggcaggca tggatttgat gtcgctgctg
     5581 cacaccatga aagcgggctt cggcaacacg ctgggagaac tggctatcat cgtggtgttc
     5641 ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat agcacatacg
     5701 ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg tgattatcat cggccttatt
     5761 ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcgccgct ggttattgtt
     5821 attgccgccg aagctaaaat tccgttcctg aaactggcga tcccggcagt agcagctgcc
     5881 actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt gaatgcttat
     5941 ggcgcggata tggggatggt ttatatctat ggcgtactgg tgacgatccc aagtgtaatc
     6001 tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac gccatcattc
     6061 ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt ttcgattctg
     6121 gtgccgctga tcccggcgat cattatgatc tctaccacca tcgccaatat ctggctggta
     6181 aaagataccc ctgcctggga agtggttaac tttattggtt cctcgccgat tgcgatgttt
     6241 attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc gtggtcatga catgcagtgg
     6301 gtgatgaacg cttttgaaag cgcggtgaag agtattgcaa tggtgattct gatcatcggt
     6361 gcgggtggcg tgctgaagca gaccatcatc gacaccggca ttggcgacac catcggcatg
     6421 ttgatgtccc acggcaatat ctcgccctac attatggcat ggctgatcac tgtgctaatt
     6481 cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg ccgccgggat tatcagtgct
     6541 gcaatccttg atccagcaac cggtcagctg gttggcgtga atccggcgct gctggtactg
     6601 gcgacggctg cgggttccaa caccctcacc cacattaatg atgcatcttt ctggttgttc
     6661 aaaggttact ttgacctgtc agtaaaagac acgttgaaaa cctggggtct gctggagctg
     6721 gtcaactccg tggttgggct gattattgtg ctgattatta gcatggtagc gtaaaagaac
     6781 accagagcct gccaatggca ggctcagact gatgactaaa gtctaaaaat gtgcccggac
     6841 agtcccctcg cccctccggg gagagggtta gggtgagggg aacagaccag taaagtgcgc
     6901 tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc
     6961 aggttttgta ggtctgatag cgtagcgcct caggtaccag cattagcaat gcgctgtaat
     7021 ttttttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct
     7081 gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa gttcctactg
     7141 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
     7201 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
     7261 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
     7321 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
     7381 cacaaccgcc agtgggcacc accacatcat tattggcgta aatattccac acaaactgaa
     7441 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgaaccag
     7501 ctttaatagc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
     7561 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat
     7621 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccgtaagccg
     7681 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
     7741 tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta
     7801 cataaacatt agcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg
     7861 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaatagctc
     7921 gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
     7981 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
     8041 tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg
     8101 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     8161 tatccggtgc tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     8221 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacataaga
     8281 ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac cgtacacggt
     8341 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt
     8401 gcgagcgcca acattgccgc caatacatac ccacgtttgc accatttcat gagcatctcc
     8461 agttactgat attcaagagt gaaggtagcc gtagcattga tatgccccgc agtgacaggc
     8521 acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg
     8581 gtcgtccacg aaatcgcaga cgatggagca ttaaggggta tttgattttg ctgctcattc
     8641 agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc aagcaggttg
     8701 gcattgtggc tatctgcaac gccggtaaac ccaactttta cggcagaaac ggcattacca
     8761 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gttgttaaat
     8821 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
     8881 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
     8941 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     9001 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     9061 tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     9121 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
     9181 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg agttagcgtc
     9241 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
     9301 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccaaggt attggtatcc
     9361 agcgccactc tattttcccg atattcagtg gcataaggca gcacggcata accacgccag
     9421 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     9481 agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccaatacc
     9541 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     9601 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
     9661 tcgcctcccc cggcatagcc ggtttgcacg ctataactga ggttgttgtc ttccagcaac
     9721 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag
     9781 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     9841 atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg
     9901 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc
     9961 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
    10021 gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg
    10081 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
    10141 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
    10201 cggtaaccca ctaactgaat attcgtgcct gattcattga gcgatttgtt atagagaaaa
    10261 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
    10321 tccacagaca gagcgcccag tgcctccatg tttttcccga taccgaaatt aaaagcacga
    10381 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
    10441 aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct
    10501 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
    10561 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
    10621 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
    10681 atgccatatc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
    10741 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
    10801 ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc caacgtcagc
    10861 cgggaacgta aaggaattat gtctcgctca atccaggtaa tgatatgctg ccatttattt
    10921 ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc
    10981 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
    11041 atcc
//
LOCUS       DAJTNP010000117        10948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTNP010000117 DAJTNP010000000
VERSION     DAJTNP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO160_003430"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO160_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5525421.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO160_003431"
     CDS             complement(1433..1759)
                     /locus_tag="OO160_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5525422.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO160_003432"
     CDS             1894..2235
                     /locus_tag="OO160_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5525423.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO160_003433"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO160_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO160_003434"
     CDS             complement(2833..3543)
                     /locus_tag="OO160_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5525424.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO160_003435"
     CDS             3651..3956
                     /locus_tag="OO160_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5525425.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO160_003436"
     CDS             4155..4475
                     /locus_tag="OO160_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5525426.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO160_003437"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO160_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO160_003438"
     CDS             complement(6299..7288)
                     /locus_tag="OO160_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525427.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO160_003439"
     CDS             complement(7373..7864)
                     /locus_tag="OO160_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525428.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO160_003440"
     CDS             complement(8185..8685)
                     /locus_tag="OO160_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525429.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO160_003441"
     CDS             complement(8675..9190)
                     /locus_tag="OO160_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525430.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO160_003442"
     CDS             complement(9621..9989)
                     /locus_tag="OO160_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525431.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..>10948)
                     /locus_tag="OO160_003443"
     CDS             complement(10401..>10948)
                     /locus_tag="OO160_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5525432.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2809 a         2784 c         2706 g         2649 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg gcaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca
    10861 gaccgagcgc cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg
    10921 tgagccgtgt gtcgtgtgaa ctgatact
//
LOCUS       DAJTNP010000118        10917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTNP010000118 DAJTNP010000000
VERSION     DAJTNP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            155..505
                     /locus_tag="OO160_003444"
     CDS             155..505
                     /locus_tag="OO160_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5525433.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            558..953
                     /locus_tag="OO160_003445"
     CDS             558..953
                     /locus_tag="OO160_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5525434.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            994..1737
                     /gene="cmoA"
                     /locus_tag="OO160_003446"
     CDS             994..1737
                     /gene="cmoA"
                     /locus_tag="OO160_003446"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5525435.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1734..2705
                     /gene="cmoB"
                     /locus_tag="OO160_003447"
     CDS             1734..2705
                     /gene="cmoB"
                     /locus_tag="OO160_003447"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5525436.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="OO160_003448"
     CDS             complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="OO160_003448"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5525437.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5324..6424)
                     /locus_tag="OO160_003449"
     CDS             complement(5324..6424)
                     /locus_tag="OO160_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5525438.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="OO160_003450"
     CDS             complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="OO160_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5525439.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7572..8138)
                     /locus_tag="OO160_003451"
     CDS             complement(7572..8138)
                     /locus_tag="OO160_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5525440.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8354..10087
                     /gene="argS"
                     /locus_tag="OO160_003452"
     CDS             8354..10087
                     /gene="argS"
                     /locus_tag="OO160_003452"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5525441.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10264..10752
                     /locus_tag="OO160_003453"
     CDS             10264..10752
                     /locus_tag="OO160_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5525442.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2666 a         2785 c         2604 g         2862 t
ORIGIN      
        1 ataatgggct tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac
       61 tcatagctaa ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt
      121 aggaaccatc tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt
      181 tcatgcatca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
      241 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
      301 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
      361 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
      421 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
      481 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
      541 ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa
      601 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
      661 gtggttttag cgaactgcaa agcgctattt gcattcatgg taacgcggtg gaatatattc
      721 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
      781 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
      841 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
      901 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
      961 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     1021 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg
     1081 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     1141 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     1201 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     1261 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     1321 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     1381 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     1441 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     1501 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     1561 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     1621 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     1681 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     1741 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     1801 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     1861 ccaacacggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     1921 taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta
     1981 tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     2041 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     2101 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     2161 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     2221 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     2281 agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     2341 gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg
     2401 tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt
     2461 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     2521 agaagtgtgg ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc
     2581 agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc
     2641 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     2701 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc
     2761 ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag
     2821 ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa
     2881 cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
     2941 ccagcgcaga gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta
     3001 acacgttcgc actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat
     3061 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     3121 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt
     3181 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     3241 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     3301 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     3361 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     3421 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     3481 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     3541 tgacgcgttg cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca
     3601 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     3661 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     3721 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     3781 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     3841 cgacgatcat ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg
     3901 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     3961 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     4021 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     4081 tttccggcag cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga
     4141 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     4201 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     4261 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     4321 tttccgccgt ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     4381 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     4441 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     4501 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     4561 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     4621 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
     4681 tgttttccag aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca
     4741 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
     4801 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
     4861 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct
     4921 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
     4981 cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat
     5041 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
     5101 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca
     5161 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
     5221 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
     5281 cacgtcttgt taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
     5341 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc
     5401 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
     5461 cggcaataga ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
     5521 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
     5581 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
     5641 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
     5701 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
     5761 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
     5821 aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg
     5881 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
     5941 tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
     6001 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
     6061 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
     6121 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
     6181 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
     6241 tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
     6301 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
     6361 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
     6421 gcatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
     6481 cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg
     6541 atttattaca atgtaatcat taattgctaa ggaataaccc agttgctatt tagaatatat
     6601 atttatcagt tttagcaatt taaatcccat aatcaatgtg aatataaaaa atatttttag
     6661 cacaacgcta tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata
     6721 ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat
     6781 catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc
     6841 ctttcatttc agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt
     6901 catctgatga catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc
     6961 ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac
     7021 ggactcctgc tccagccata atgattggag catcacgatg ggcaataagt tccataattt
     7081 ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca
     7141 attccgcaag attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa
     7201 aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat
     7261 tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc
     7321 gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg
     7381 ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca
     7441 acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt
     7501 tttgctgcgc cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt
     7561 ttgctcctta attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga
     7621 aacttaatag ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc
     7681 tcaccttttg gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa
     7741 agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata
     7801 tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacattggaa
     7861 aactgccagt gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca
     7921 ttgatcattt tttctgacaa aagctcgcca cactgttcaa aaccgcgacg ccagcgctca
     7981 gcggtgacgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc
     8041 aaccccagac ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca
     8101 atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta
     8161 aaagcgttaa tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg
     8221 atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc
     8281 agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat
     8341 gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat
     8401 tgcggcaggc gcgcctgcgg attgcgaacc gcagattcgt cagtcagcaa aagttcagtt
     8461 cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg
     8521 acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga
     8581 gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt
     8641 tcagcaggcg ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt
     8701 ggttgactac tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac
     8761 cattattggt gacacagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc
     8821 aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca
     8881 gcagcaggaa aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc
     8941 gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa
     9001 actgcaaagc ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat
     9061 gacgcagaac cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat
     9121 gggcgaaagc ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg
     9181 tctggcagta gaaagcgaag gggcgaccgt cgtattcctt gatgtgttta aaaacaagga
     9241 aggcgaaccg atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac
     9301 tgatatcgcc tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta
     9361 catcgactcc cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg
     9421 ctatgtaccg gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga
     9481 cggcaaaccg ttcaaaaccc gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga
     9541 agccctggag cgtgcacgtc gtctggtggc agaaaagaac ccggatatgc cagctgacga
     9601 gctggaaaaa ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa
     9661 aaaccgcacc acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac
     9721 cgcgccatac atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aagcagaaat
     9781 taacgaagag caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact
     9841 ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc
     9901 gcatgtaatg tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca
     9961 ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca
    10021 actgacagcg aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg
    10081 tatgtaatcg atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt
    10141 attgccatct aaatgtattc tgaaaacgga catgccattg ttttctcact gttgagtaag
    10201 aggccagaag cgtaatatcc aggcccagga aaacgataac ggttcaattt aaggaatacc
    10261 gaagtgttta aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa
    10321 gatacggtta tagtaaacga ccatgacact tcagccatca aagattgttg gcaaaaaaat
    10381 tcagatgatg atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc
    10441 gtcgatgcgc aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca
    10501 cgcacaggtg caaaaaaact tgatactgaa caacttaact tgcttaaaaa atcacagcga
    10561 gcctggctgg attttaggga caaagaatgt gaattaatcc tttcaaatga ggatgttcag
    10621 gatttaagtg acccctattc tgaatcagaa tggctatcat gtatgatcat acagaccaat
    10681 acgcgaactc gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg
    10741 acaagaggat aattcacatc tttttggcat gttttgttgc aagctattcc tgataaatga
    10801 ttgcaacaaa acatccagcc ttttgactga gttattaaac atactcgcga gcgcgtaatt
    10861 ttttgtcttt cagcgataat tcacaatcac ttcattccgt tgtaccttta gcggtgg
//
LOCUS       DAJTNP010000119        10862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTNP010000119 DAJTNP010000000
VERSION     DAJTNP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..148
                     /locus_tag="OO160_003454"
     CDS             <1..148
                     /locus_tag="OO160_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525443.1"
                     /translation="VGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAMTALLMKD
                     NQRS"
     gene            250..1704
                     /gene="amn"
                     /locus_tag="OO160_003455"
     CDS             250..1704
                     /gene="amn"
                     /locus_tag="OO160_003455"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5525444.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2047..2763
                     /locus_tag="OO160_003456"
     CDS             2047..2763
                     /locus_tag="OO160_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5525445.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3219..3294)
                     /locus_tag="OO160_003457"
     tRNA            complement(3219..3294)
                     /locus_tag="OO160_003457"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3259..3261),aa:Asn,seq:gtt)
     gene            complement(3395..5022)
                     /gene="yeeO"
                     /locus_tag="OO160_003458"
                     /pseudo
     CDS             complement(3395..5022)
                     /gene="yeeO"
                     /locus_tag="OO160_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5027..5102
                     /locus_tag="OO160_003459"
     tRNA            5027..5102
                     /locus_tag="OO160_003459"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5060..5062,aa:Asn,seq:gtt)
     gene            complement(5140..6090)
                     /gene="cbl"
                     /locus_tag="OO160_003460"
     CDS             complement(5140..6090)
                     /gene="cbl"
                     /locus_tag="OO160_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5525446.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6192..7109)
                     /gene="nac"
                     /locus_tag="OO160_003461"
     CDS             complement(6192..7109)
                     /gene="nac"
                     /locus_tag="OO160_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5525447.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7437..7512
                     /locus_tag="OO160_003462"
     tRNA            7437..7512
                     /locus_tag="OO160_003462"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7470..7472,aa:Asn,seq:gtt)
     gene            complement(7568..8503)
                     /gene="ldtA"
                     /locus_tag="OO160_003463"
     CDS             complement(7568..8503)
                     /gene="ldtA"
                     /locus_tag="OO160_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5525448.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8565..9644)
                     /gene="cobT"
                     /locus_tag="OO160_003464"
     CDS             complement(8565..9644)
                     /gene="cobT"
                     /locus_tag="OO160_003464"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5525449.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9656..10399)
                     /gene="cobS"
                     /locus_tag="OO160_003465"
     CDS             complement(9656..10399)
                     /gene="cobS"
                     /locus_tag="OO160_003465"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5525450.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10396..>10862)
                     /gene="cobU"
                     /locus_tag="OO160_003466"
     CDS             complement(10396..>10862)
                     /gene="cobU"
                     /locus_tag="OO160_003466"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5525451.1"
                     /translation="LYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELIHAD
                     INPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAK
                     VVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3026 a         2765 c         2345 g         2726 t
ORIGIN      
        1 ggttggcggt ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa
       61 ctggcatagc atcgccattt atttgctggc tggatgcctg atttctgcaa tgaccgcttt
      121 attgatgaaa gacaatcaac gctcgtgata gcctggcgaa gatcatccga tcttcgcctt
      181 atacttttgt ttcacattcc tgtgacatac tatcggatgt gcggtaattg tatggaacag
      241 gagacacaca tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc
      301 gacgcgctgt atgagcaatc tgtagttgct ttacgcaacg ccattggcaa atatattaca
      361 attggcgaat tacctgatga aaacgcccgt aaacaaggtc tttttgtcta tccatcactg
      421 accgtaacct gggacggtag cacaacaaat ccccccaaaa cgcgcgcatt tggtcgtttt
      481 actcacgcag gcagctacac caccacgatt actcgcccta ctctctttcg ttcgtatctg
      541 aatgagcaac taacgttgct ttatcaggat tatggtgcgc atatctctgt gcaaccctcg
      601 caacatgaaa tcccttatcc ttatgtcatc gatggctctg aattgacact tgatcgctca
      661 atgagcgctg atttaactcg ctacttcccg acaacagaac tggcgcaaat cggcgatgaa
      721 actgcagacg gcatttatca tccaacggaa ttctccccgc tatcgcattt tgatgcgcgc
      781 cgcgtcgatt tttccctcgc acggttgcgc cattataccg gtacgccagt agaacatttt
      841 caaccgttcg tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga
      901 tgcagccaga tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac
      961 tggatcactg ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa
     1021 caccagatgc cagcatggca tttaattacc gctgatggtc agggtattac tctggtgaat
     1081 attggcgtgg gaccgtccaa tgctaaaacc atctgcgatc atctggcagt gctacgcccg
     1141 gatatctggt tgatgattgg tcactgtggc ggattacgtg aaagccaggc cattggagat
     1201 tatgtccttg cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc
     1261 gatataccta ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc taccaagtta
     1321 gtcagcggca ggcctggtga ggaagtcaaa cagcggctgc gtacaggtac tgtggtaacc
     1381 acagatgaca ggaactggga gttacgttac tcagcttctg cacttcgttt taacttaagc
     1441 cgggccgtag caattgatat ggaaagtgca accattgccg cgcaaggata tcgtttccgc
     1501 gtgccatacg ggacactact gtgtgtttca gataaaccgt tacatggtga gattaagctt
     1561 cccggccagg ctaaccgttt ttatgaaggc gctatttccg aacacctgca aattggcatt
     1621 cgggcgatcg atttgctgcg cgcagaaggc gaccgactgc attcacgtaa attacgaacc
     1681 tttaatgagc cgccgttccg ataataagtt atgaaccttc ttatcagaaa tagatgtgaa
     1741 ggattaagta agacatctgg caaatagcct gcaataaacg cgggctgctt gcttccttgg
     1801 gcagatgcga gttttattat cgtcttaatg atttccacat attaaaagca agtatgcttt
     1861 caaaacacaa ttataaaaaa tcccgccaac aacattagtt tttataaaat taaatataag
     1921 attatggctt tagaatattt ttatttctaa tagacgagat ttttcctgtt atgatataat
     1981 atgccgaatt aacacatgtt aacgatttac cagtaatgta aataaatttt cgaggagatc
     2041 attctagtgg gacgtaaatg ggccaatatt gttgctaaaa aaacggctaa agacggtgca
     2101 acgtctaaaa tttatgcaaa attcggtgta gaaatctatg ctgctgccaa acaaggtgaa
     2161 cccgatccag aattaaacac atctttaaaa ttcgttattg aacgtgcaaa gcaggcacaa
     2221 gttccaaagc acgttattga taaagcaatt gataaagcca aaggcggcgg agatgaaacg
     2281 ttcgtacagg gacgttatga aggctttggt cctaatggct caatgattat tgctgagaca
     2341 ttgacttcaa atgttaaccg tacgattgct aacgttcgca caattttcaa taaaaaaggc
     2401 ggcaatatcg gagcggcagg ttctgtcagc tatatgtttg acaatacggg tgtgattgta
     2461 tttaaaggaa cagaccctga ccatattttt gaaattttgc ttgaagctga agttgatgtt
     2521 cgtgatgtaa ctgaagaaga aggtaatatt gttatttata ctgaacctgc tgaccttcat
     2581 aaaggaatcg cggcgctaaa agcagctgga atcactgagt tctcaacaac agaattagaa
     2641 atgattgctc aatctgaagt tgagctttcc ccagaagatt tagaaatctt tgaagggctt
     2701 gttgatgccc ttgaagatga cgacgatgta caaaaagttt atcataacgt cgcaaatctc
     2761 taattatatt ttaaagaaat ctgtctttat ggcagatttc tttaatctca tataattctt
     2821 ataaaaaata taatatttaa ctcgtcatat tgcttataaa aaatccccgt ccccagagaa
     2881 ataatattta ttaaaatggc aggtcttctt tttctgatta ccgaagacaa agtggcaatt
     2941 acgtatagtt tcccgataaa gacgcgacag ctacgtcccg cataaggcat ttttctcttt
     3001 atctttgtac ggtacttcat ggaacagaat ttttgacctt gtgaatcgtg atatctgttg
     3061 gggaggggca atttgctcac tgaagcgtga gactcgatta agcgcacgaa acacagaaat
     3121 caaaaaaccc ggccactttt ttacaaagtg gccgggtaaa aataattttt attttttaac
     3181 tgtcctgagt ctcatagaga tgtctcaaaa ctaaaatttg gctcctctga ctggactcga
     3241 accagtgaca tacggattaa cagtccgccg ttctaccgac tgaactacag aggaatcgtg
     3301 agaacgaggc gaatattagc gatgcccacc cacaatgtca aagcctgttt tttaaatttg
     3361 aaatcgtttg ctgaaataat ctgcattttg tcgtttattc cgacacaact ggcttttttt
     3421 cacacttttg cggctcggat cgagggtatt tccacagcca acgtccagta accattcgcc
     3481 agtaaaacag cacggcccgc acagcccagt cggcaaacat tcccatccag acaccaacca
     3541 caccccagcc aagcatgatt ccctgcacat aaccgactac aacccggcaa ccccacatgc
     3601 tcaacataga aacccacatg gcgtaacggg catcacgagc acccttaaat ccagcgggta
     3661 gcacccatga ggcggaccaa ataggcataa ataaagcatt tagctaaatc agaatcacaa
     3721 cgacgtgttt aacctgtgga tcctgggtgt aaaacgatgc cataaccccg gcaaagggag
     3781 ccgttagcca ggcgatggcc gttaatccaa gagtggacag ccagaacaca tgccgcaact
     3841 gaatctctgc ttgcgctatc tgccctaccc ccaaccttcg gcctgtaatg atcgtagaag
     3901 cagaaccgag cgcacttccg ggtaagttga taagagccgc aattgagaac gcgataaaat
     3961 ttccggcaat aacgctggtc cccatcccgg caacgaacat ttgggttaat aaccgaccac
     4021 tggtaaataa cactgattcg acactcgctg gaataccaat ccccatgact tcccagataa
     4081 tgctaaaatt cagcggttta aaatagctct ttaacgaaat ccttagcgca ggattaaaac
     4141 caatcgccag cacccacaaa attgcaactg cgccaatata acgagaaatg gttaaaccca
     4201 acccagcccc gaccaggaga aaaggccgta aatcaatatg ccgctaataa taatattaag
     4261 aatattcagg ctaccgttaa tcagtagcgg tattttcgta ttccctgcac cacgaagtgc
     4321 cccgctacca ataagagtga tggcagcggc tggataactg agtacggtca gctccagata
     4381 agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat caataatttg
     4441 ttcgccaaaa tgatgaatta gcgttgccaa cagtacggca aacaacgtca tgatcaccaa
     4501 tgactgccgc gtcgccaccc tcgctcgtcg tcgatcccgc ttaccgagac taaatgctac
     4561 aacgacagta gtaccaagat cgatagctgc aaaaaaagcc ataatgacca tattgaagct
     4621 gtccgccaac cccacgccgg ccatcgcatc ttttcccagc caactgacca gaaaagtgct
     4681 caggaccccc atcaacagga cacaggcgtt ctccatgaag ataggaacag caagcggggt
     4741 tatctcgcgc tagaacaaca ctttgtagct cttgcgttta gagtgccagc gagtgccgtg
     4801 aacaacctgg cgtaaagcag aggagatatt caaagccgac cttaattgca gaaagtgaaa
     4861 ccacatttca aatgatgagg gagaatcagc aaagctgcaa agattttcgc caacaaattg
     4921 tctgcaaatg caacaaactg ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg
     4981 gtttgacaaa atatttttcg ccgttaagat gtgcctcaac agcgattcct ctgtagttca
     5041 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     5101 caaatttctg aaaagcccgc ttttatagcg ggctttttgc tatatctgat aatcaatttc
     5161 ctcttcactg ttttccatca cctggcgctt gatatcctca actgacagtc ctgcattaca
     5221 aagttccaga aagcgccaaa catagttacg ctgaagttgt cctcgcttca gtcccaacca
     5281 gacagtatta gcatcgaaaa gatgccgcgt atccaggcgg attaaattct cttcctcttg
     5341 ttcgccgctg gattgttcgg caactaatcc gatcccaagc ccaagagcaa cataggtttt
     5401 aatgacatca gaatcctgtg cacttaatac aatatctgcc ggcaaacctt tgcgggtaaa
     5461 tgcgtcatca gtgcgtgagc gccccgtaat tccctgtcgg taagtaatta acggccactt
     5521 cgctattgat tccagcgtca atggtgtaat ttgcgttaag ggatgatcga gtggaacaag
     5581 caaactatgg tgccaacaaa accatgggaa ggcgacgagc tgcgggtcat tactcaaacg
     5641 ctcgctggcg ataccaatat cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg
     5701 cgaaccctgg attagctcga gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac
     5761 ctctggcaag ctataacgtg cctgagtatg cgtcgttgca atagtgagaa caccagacgt
     5821 atcgttggta aacaggtctg caagccgacg aacattactg gcttcattca gaatacgttc
     5881 tgcaatgacc agtaatgctt tgcccggttc agtcatcccc agcagtcgct tacctcgtcg
     5941 aacaaatatt tcgatgccaa attcatcctc cagttcccga atatgacggc taacgcctga
     6001 ctgtgaggta aaaagcatat tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc
     6061 gcggattatc tttagttgtt ggaaattcac ggtaaactcc gggcagttca gatatcccgt
     6121 tattgttaaa gtctaatgcc cggcataaca aataataaaa acccgcatct tattccatcc
     6181 cgatataacg cttagctcac caattgccac tgcctttttt ccatcactgg agaactaatc
     6241 actgacatta acaactcttt cactgcctgt gcctgtggtg acaagtttgc tctggcgggt
     6301 aaatttaagg acaaagagag actcattgaa ggagtggtaa tgcgtgacat ccacccattt
     6361 actgcgccac ataacgaacg cgcagccgat tcaggtaata ctgcaacgcc catgccgctg
     6421 gcaatcgctg cggtaagcgt ggcaatagac tcaatttcac caatgacttt tgccgtgagc
     6481 cgccgtaggg aaaaagcctc atcaacacga agtctaacag cgctgtaatc actggggaga
     6541 aaaaggttca tttgcgcaat agcattaaca tcaacgtttt gccccgggca atcttgagtt
     6601 cctaccagga aaagatcttc tttcagcaaa gcctgactgg ataccccagc cacaggggaa
     6661 tgctcataaa tcaccgccat atcgagttgg tgatttatca gtttttcgtt aagcactgca
     6721 ccactatttt catgaagata gataacgacc tccgggaatt cagcgcgaac cgcctgtaat
     6781 aagggcatgg tgatggatga cgcagcggtt cctggcgcaa agccaatcga gacttgcccc
     6841 gataatgact gaccaacgtt atgcaccgcc agttgggcct gctcacactg acgtaaaatg
     6901 gcccgtgcat gggtatagag aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg
     6961 cgaatcaaaa gctgttgatt taactcacct tccagtatgg caacctgttg gctgagcgct
     7021 ggttgtgcga tatgcaatac ttcagcagcc tgggtcaggc taccaatatc tacaattttt
     7081 acgaagtatt tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa tcggcccaag
     7141 tgccgccatt acttacaacc agtatgcaag atgcctgcca gttctatttt ggcgttgatg
     7201 cgcaagctaa ccaactgtca aataagagat tatgatagat tcgtcattgg ccctttttat
     7261 cagcagtcgc attcacctgc cctataaaag gagggtatgc accacgatgg ttcattaccc
     7321 aataagattg aaagctcacc actttgttga aattggcagc aaacaaacaa aaaaaatgca
     7381 tttcaccctt tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct
     7441 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
     7501 tcagaggagc caaattcaaa aaagcctgct ttcgagcagg ctttttactt tctaattacc
     7561 aacgctctta aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgat
     7621 gttgcaacgc agcatttgcg cgatttacat caacttcttg cccgttgata aacgcccgca
     7681 aagatggggt taccggcaat ggcacttttc ggtcagactc atattctgca cgattgcgcg
     7741 acagcggctc atgaacttcc agccactttg aaccatccgg ttccgttgtg tatttcactg
     7801 gctgatcaat aatttgtaca cgagtgccta caggaacatt atcaaacaga tatttgatat
     7861 cgtcattacg cagacgaata cagccctgac ttacacggag cccaatacca aaattggcat
     7921 tggtaccgtg gatggcatac aacctgccaa tataaatcgc gtacagcccc atggggttat
     7981 cgggccccgc aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccgga
     8041 tgttcggcgt tggcgtccag gttggtgctt cttgtttacg ttcaacggta gtcacccagt
     8101 tacgcggggt ttctcgccca gcctggccga taccaatagg aaagacttcc actgtattac
     8161 tgtctggcgg atagtaataa agacgcattt cagcgacgtt aaccacaatc cctttacgaa
     8221 cagtggcggg caaaatcagt tgctgcggaa tggtgagttg tgagccaggc ttcggcaaaa
     8281 aaacatcagc gcccggattc gcttccagca tattacttaa cccttgcccg tattgtgcgg
     8341 caaaagtctc cagcggctga gtattgtgat caggaacagt tacagtaagc gactgcccca
     8401 ctaaacggct accctctgga ggtaatggat aagttaccgc caggctattt tggctggcaa
     8461 aaagcagagc aaatgagcaa agaatattta cacgacgcat catgtccctt tcctaagtcg
     8521 cgaagactat ccgataagta tagcttttat cagacttttc gtttttagct gttcaaatca
     8581 gaagtcgtat tccccggtag aacaatatta ctggcagcaa gttcgcccat gttgttgtat
     8641 atcgcacaag cagcttcgat gatggacatc gccagagctg caccactccc ctcacctaaa
     8701 cgcatatcca tattgagata aggctccagc cccaaatgcg agagcgctat acgcgcgcct
     8761 ttttctgccg acaagtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag
     8821 gctgcgagcg cagcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa
     8881 gcagcaccta acatcactcc agctattccg accaaatcaa atccacccac ttttgccagg
     8941 acatcaacac catcctgagg atttggttga ttcaacgtaa tcgcccgacg cacaacatca
     9001 attttattag ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga
     9061 tcccggccag tgattgtgct gactattgcc gctgccggtg tcgtgtttgc catccccagt
     9121 tcacctacac caaacagcgt gacaccgttt ttcgccagct cccgcgtata acatatgacg
     9181 tccaaaagca acttttcagc ctgacggcga ctcattgccg gagctgaagc aatattgccg
     9241 ctacctcgtg cgacacgcat gttgataagc ccggggatag gctcagcagt atcaatacca
     9301 acatcaatta cgtggacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt
     9361 ccacgggtca tattttcagc ctgtatggct gtcacttctt ttggagaaat agcgacccct
     9421 tcctcccaga cgccgtgatc ggcacacata accagtaccg cttttttgcc cacatgcggt
     9481 atgccattca cccccggcat tcctgccagt tgtatggcaa gcgcctccag ctttcctagg
     9541 ctaccaacag gtttgaataa cccgtcaaca tgccgttgtg cacgcgacat agcggcagaa
     9601 ttgatggctg ggatcgtatt cagtaaatcg gcaattattt gcatgtcacg tttctttata
     9661 gcagtgccag caggaagacc agttcaccaa gctctatggc tgctcccagc gtatcgcccg
     9721 tttgcccgcc cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca
     9781 ccaccatagc agccacacca tgcataccca gcaataatac ggcagcaaaa atagcggcta
     9841 agccgagtgt gacgcaggtt tgtcgcccat caattttgcc aatgaacaca ttgccaagcc
     9901 cttcttcccg cgcataacga tgacgataca tcaataatgc ggcagtcccg cggctgaccg
     9961 cacatgctgc cgccagcgat gcaaggatcg gttcgccacg cagagccaac tcgcttaaca
    10021 ccagaatctt tgccagtacc acaaaaatca atgccagacc gccgtgggtg cctaaacggc
    10081 tatcacgcat gatttccaac atgcgatcgc gactacgtgc agaaaatacg ccatcacagg
    10141 tatcagcgag gccgtccaga tggaattccc cggtcatcag cgccagcaca agtacgctaa
    10201 acagtgccgc cagtggtgcg ccacaccatg cctgcagcac catgaagacc agcccgctaa
    10261 tcgcgccaag caataatcca atcaaaggaa aagtaataat accgcgagaa taatgttcga
    10321 aatccagtcc ctgggaccag cgacgcggta caggcagacg cgtaataaat gagagcatcg
    10381 cccaaaataa tttgctcatt tgatttttac tccaataccc gaaaccacca gccatacttc
    10441 atttgccgca gcggccaact gctgatttac ccgcccggca atatcacgaa aatgtcgtgc
    10501 cagacgactc tccggcacaa tccccattcc cacttcgtta gtcactaata caacctttgc
    10561 ggggcaacgt tggcaggcag caatcaacga ctgaatctca gcattaatcg cctgttccat
    10621 cgcctgataa tcccattcat cagggtcttt atcgccgcca taatcaaaca acagattagt
    10681 caccattgtg gtaacgcatt ccagcaacac agcctcattc gggttaatgt ctgcatgaat
    10741 taattcatca agatgttgcc agcgctccac tgtgcgccag tgctccgggc ggctttgccg
    10801 atgatgttct atccgtgcag ccatctcatc atcaaggatt tgcgaggtag cgatatacag
    10861 aa
//
LOCUS       DAJTNP010000120        10564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTNP010000120 DAJTNP010000000
VERSION     DAJTNP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            3..332
                     /locus_tag="OO160_003467"
     CDS             3..332
                     /locus_tag="OO160_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5525452.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            304..3345
                     /locus_tag="OO160_003468"
     CDS             304..3345
                     /locus_tag="OO160_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5525453.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            3345..3674
                     /locus_tag="OO160_003469"
     CDS             3345..3674
                     /locus_tag="OO160_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5525454.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            3674..4372
                     /locus_tag="OO160_003470"
     CDS             3674..4372
                     /locus_tag="OO160_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5525455.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            4377..5120
                     /locus_tag="OO160_003471"
     CDS             4377..5120
                     /locus_tag="OO160_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5525456.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            5057..5659
                     /locus_tag="OO160_003472"
     CDS             5057..5659
                     /locus_tag="OO160_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5525457.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            5720..9199
                     /locus_tag="OO160_003473"
     CDS             5720..9199
                     /locus_tag="OO160_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5525458.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            9267..9866
                     /locus_tag="OO160_003474"
     CDS             9267..9866
                     /locus_tag="OO160_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5525459.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            9930..>10564
                     /locus_tag="OO160_003475"
     CDS             9930..>10564
                     /locus_tag="OO160_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5525460.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2479 a         2512 c         3405 g         2168 t
ORIGIN      
        1 aaatgcagtt tgtcatgcgg cttgcccggg agttccggcg ggcagactgg cggcggatgc
       61 tgtcggatat gtcggccact gagcttggtg agtggggcga ttatttccgg atgcagagct
      121 tcagtgatgt gtggatggat gcgcagtttg cctcgctgaa ggcattgatc gtgagaatgg
      181 tgtccggcag cagcgatgct gcggtggctg atttcagcct tttaccggaa gagaacggga
      241 taccggagcg aacggacgaa gaactgatgc atcttgggga aggtatttcc ggaggtgtgc
      301 gttatggacc agatagccaa cctggtcatt gatttgggga ttgatgcggc agagtttaaa
      361 aatgaaattc cccgtatcaa aaaccttctg aatggtgcag ccagcgatgc agaacggtct
      421 tctgcccgta tgcagcgttt tatggagcgt cagactcagg cggcccggca gacaatgcag
      481 gcggcttctt cggctgcaac agcagcatca gcccatgcgc agacggtgga gaagaatgca
      541 cgggctcatg aacgcatggc ccgtgaggtg gaacaaaccc gtctgcgcgt ggatgccctg
      601 aatcagaaaa tgcgcgagga acaggcgcag gccagggcac tggcggaggc gcaggataaa
      661 gcggctgccg ccttttatcg ccagattgac agtgtgaaac aggccggtgc ggggcttcag
      721 gaattacagc gtattcagca gcagatccga caggccagaa acagtggcgg ggttggtcag
      781 caggattatc tggcgctgat ttcggagatc acggcgaaaa cccgtgccct gacgcaggca
      841 gaggaacagg ccacccggca gaaagcggcg tttatccgcc agcttaaaga gcaggcaacc
      901 cgccagaatc tgtcgtcttc tgagttgctt cgcgccaggg cggctcagct gggggtaagc
      961 agtgctgcag aagtgtatat ccgcaaaatg gagcgggcag gaaaagccac acattcgctg
     1021 gggctgaaaa gtgcggcagc ccggcgggag ctgggggtgt taatcagtca gatggcgcga
     1081 ggcaattttg gtgcgctgag gggatccggg ataacgctgg ctaaccgtgc cggatggaca
     1141 ggcgcactga tgtcgccgaa aggcatgatg actggcggcg ttattggcgg acttgtcgcg
     1201 gcggtcctgg gtctgggtaa agcctggcat gacggccgga aggagggcga gaaatttaac
     1261 cgtcagctgg cgctgacggg acattatgcc ggtgtcactg tcgggcagtt gtggaaactc
     1321 agccgggcca tatccgggaa tggtatcacg caacatgcgg cagccggtgc gctggcacag
     1381 gtggtgggca gtggggcatt tcatggaaac gatattggta tggtggcgaa agctgccgca
     1441 cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt taagcggctg
     1501 aaggatgatc ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt tttgaatgcc
     1561 actcagcttg agcagatacg cgtccttggg gagcaggggc ggtccagtga tgcggcccgg
     1621 atcgccatgt ctgcgctggc agaggaaacc ggtaaacgca catctgatat tgataataat
     1681 ctcaatgcgc tgggtagtac gctgcaaacc ttgtctgact ggtggaagca gttctgggat
     1741 gcggccatga atattggtcg tgaagactcg ctggatgcgc agattgatgc gttacaggaa
     1801 aaaattcagc gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt ggagtacgat
     1861 cagcagcgtc ttaacgatct tcaggagaaa aaacgccgga aggatttgca ggatgcaaaa
     1921 gcgcaggcag aacggaatta ccaggagcaa cagaaacgcc ggaatgctga aaatgccgcg
     1981 ctgaaccgga tgaatgaaac ggaagctgca cgacatcagc gggaaattgc gcgtattaat
     2041 gccatgcagt acgccgacca ggctgtcagg gatgcggcga tacagcgtga aaatgaacgt
     2101 tacgaaaaag ccattaagaa aaatacacgg gcaacccgta atgatgaggc cacccggtta
     2161 ttgctgcagt acagtcagca gcaggcacag gtggaagggc agattgctgc tgccagacag
     2221 tcagcaggca ttgccactga aaggatgact gaagcgcata aacagcttct ggccctgcag
     2281 cagcgcatca gcgacctgga cgggaaaaag ctgacggcag atgaaaagag tgtgctggcc
     2341 cgtaagaatg agctgattca ggcgctgacg ctgctggatg tgaaacagca ggagctgcag
     2401 aaacagacag cgcttaacga cctgagaaaa aaaacggttc agctgaccag ccagctggca
     2461 gacaaagaac gtgcactgcg tgagcagcac aatctggata ttgccactgc aggtatgggg
     2521 gataagcagc ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata ccggcaacag
     2581 ttgcaacagc ttgagaatga cagtcgccag aaaggcactt acgggacgga ggactaccgg
     2641 agggctgagg aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa caaacgctac
     2701 tggcaggaac tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg ggcgtttcag
     2761 aattttacgg cggatgcgga taatgcggcg ggaacggcag aacagatgtt tacagtggca
     2821 ttcagcagtg ccggtaatgc actggcgaca ttctgtacca ccggtaagct gaattttaaa
     2881 tccttcacct cttccctgtt gtcagatatg gccagaatta tggcacagat ggccatgatg
     2941 caggcggtaa agggcgtcgg ttctttattc ggcttcacga ctaatgctga tggcggtgtt
     3001 taccagtctg ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc gacgtttttt
     3061 gcttttgcaa aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat tctgccactg
     3121 cgtcgcggtg ctgatggtaa gctgggggtt gtggcggata ctggtggttc aggcatggtg
     3181 atgtttgccc cgcagtacaa cattgagatc aataacgacg gcacgaacgg gcagataggt
     3241 ccggctgccc tgaaggtggt ttatgacctc gggaaaaaag cggccgcgga ctttatgcaa
     3301 cagcaggccc gtgatggtgg ccggttaagt ggagcatacc ggtaatggag acgtttcact
     3361 ggaaagtgcg cccggatatg aatgtggtat cagagccgaa agtggtgaca gtgaagctgg
     3421 gcgatggtta tgaacagcgt cgtgcggcgg gactgaataa ccagttgtcg acttacagcg
     3481 tgacgatacg tgttcgtaaa ggtgaacacc catctttaaa agcctttctg gaacggcacg
     3541 gtggcgtccg cgcatttcag tggacgccac cttatgactg gaaactgatc agggtggttt
     3601 gtcgtaaatg gtcggcaagc gtgggggcgt tatgggtgac tgtaacggcc gattttgaac
     3661 aggtggtgaa ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga
     3721 gcagtcagcc cgggtggagc tctgggaaat cgatctgaca gaggtcggtg gtgagcgtta
     3781 ttttttctgt aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcggcagta
     3841 tcaggcatac cccattcagg ggtcggggtt tgaactgaac ggcaggggct gtgctgcccg
     3901 tccgacactg acggtctcta acctgcacgg catggtcacc gggatggcgg aagatctgca
     3961 gagtctggtc ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt
     4021 gaacttcgtc aacggaaaca gcgacgccga tccggaacag gaggtgatca gccgctggcg
     4081 catcgagcag tgcagcgaac tgagcgcggt cagtgcctct tttgtgctgg ccacaccaac
     4141 ggagacggat ggcgcggttt tcccggggcg tatcatgctg gcgaatacct gtatgtggac
     4201 ttaccgttct gatgagtgtg gttacacggg cagggcagtg gctgacgagt tcgacaaacc
     4261 aacaacggat atccggaagg acaaatgcag caagtgtatg cgcgggtgtg agttgcgcaa
     4321 caataccggt aatttcggcg gtttcctttc catcaataaa ctttctcagt aaatccatga
     4381 cacagacaga atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg gagtcgtgtg
     4441 gcttcgtggt gagaacgccg gagggagaca ggtatcttcc cagcgagaat atctccggtg
     4501 agccggagga acggttccgg atggctccgg aggactggct gcgggcacaa atgcagggtg
     4561 agattgtggc actggtccac agtcatcccg gtggtctgcc ctggctgagt gaggctgacc
     4621 ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg acgattcaca
     4681 agttccgctg tgtgccgcat ctcaccgggc ggcgctttga gcacggggtg acggactgtt
     4741 acacgctgtt ccgggatgct tatcatctgg cggggattga gatgccggat tttcatcgcg
     4801 gggatgactg gtggcgtcac ggtcagaatc tctatctgga taatctggag gccacagggc
     4861 tgtatcaggt gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc tgttttggtt
     4921 catcggtgcc gaatcatgcc gccatttact gcggcgatgg tgagctgctg caccatattc
     4981 ctgaacaact gagtaaacga gagaggtata ccgacaaatg gcagcgacgc acacactccc
     5041 tctggcgtca ccgggaatgg cacgcatctg cctttacggg gatttgcaac gatttggccg
     5101 ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgcgcactgg ccacacagct
     5161 cccggcgttt cgtcagaaac tgagtgacgg ctggtatcag gtgcgcattg ccgggcgtga
     5221 tgcaggtgaa accgaattgt ctgcccgtct taatgagccg ctggcaaatg gtgcagtgat
     5281 ccacatagta ccgcgtctgg tgggagctaa aagtggcggt gtgtttcagg cggtgctggg
     5341 ggcagctgtt atggcggttg ctatatggat gccgggggta ggaattatgg cgagtaatct
     5401 gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc tggcaccgaa
     5461 agccagaact ccccgtacac agacaacgga taacggcaaa cagaacacct gtttctcctc
     5521 actggataac atggttgccc agggcaatgt tctgcctgtt ctgtacggtg aaatgcgcgt
     5581 ggggtcgcgg gtggtatctc aggagatcag cacggcagac gaaggggatg gtggtcaggt
     5641 tgtggtgatt ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg gcggttttgt
     5701 cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc
     5761 gaaggacaac ctgaaatcca cgcagttgct gagtgtgatt gatgccatca gcgaagggcc
     5821 ggttgacggt ccggtggatg gattaaaaag cgtgctgctg aacggtacgc cggtcctgga
     5881 tagcgagggg aagacaaact tttccggtgt tacggtggtg ttccgcgccg gcgagcagga
     5941 gcagacaccg ccggaggggt ttgaatcttc cggctcagag actgtgctgg gtacggaagt
     6001 gaaatacgac acgccgatca cccggaccat cacgtcggca aacattgatc gtctgcgcct
     6061 gaccttcggt gtgcaggcac tggtggaaac cacctcaaag ggggaccgga atccgtcgga
     6121 agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac
     6181 cattaagggc aaaaccactt cgcagtatct ggcctcggtg gtggtgggta acctgccgcc
     6241 gcgcccgttt aatatccgga tgcgcaggat gacaccggac agcaccacag accagctgca
     6301 gaacaaaacg ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt gctacccgaa
     6361 cacggcgctg gtcggcgtgc aggtggattc agagcagttc ggtaaccagc aggtgagtcg
     6421 caattatcat cttcgcgggc gcattctgca ggtgccgtcg aactataacc cgcagacgcg
     6481 gcaatacagc ggtatctggg acggaacgct taagccagca tacagcaaca acatggcctg
     6541 gtgtctgtgg gacatgctca ctcatccgcg ctacggcatg gggaaacgtc ttggtgcggc
     6601 agatgtggac aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cagtgccgga
     6661 cggatttggc ggcacggagc cgcgcatcac ctgtaacgct tacctgacca cacagcgtaa
     6721 ggcgtgggat gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg
     6781 gcagacgctg acgttcgtgc aggaccgacc gtcggataaa gtgtggacct ataaccgcag
     6841 taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctgaagga
     6901 ccgccataat gtcgttgagg tgaactggat tgatccggac aacggtcatg agacggcgac
     6961 agagcttgtg gaggacacgc aggccattgt ccgttacggt cgtaacgtca cgaagatgga
     7021 tgcctttggc tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc tgattaaaac
     7081 ggaactgctg gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc ttcgccatgt
     7141 accgggtgat gtcattgaaa tctgcgatga tgattatgcc ggtatcagca ccggtggtcg
     7201 cgtactggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc
     7261 gtcctccggt accacgctga taagcctggt tgacggaaat ggcaatccgg tcagcgtgga
     7321 ggttcagtcc gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg acggcgttgc
     7381 agaatacagc gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt tccgctgcgt
     7441 gagtatccgt gagaacgacg acggcacgta tgccatcacc gccgtgcagc atgtgccgga
     7501 aaaagaggcc atcgtggata acggggcaca ctttgacggt gaccggcgcg gcacggtgaa
     7561 tggtgtcacg ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag acagcgggga
     7621 atatcaggtg ctggcgcgat gggacacgcc gaaggtggtg aagggtgtga gcttcctgct
     7681 tcgcctgacc gtggcagcgg acgacggcag tgagcggctg gtcagcacag ccaggacgac
     7741 ggaaaccaca taccgcttca ggcagctggc gctggggaat tacagtctga cagtccgggc
     7801 ggtaaatgcc cgggggcagc agggcgatcc ggcgtcggta tcgttccgga ttgcggcacc
     7861 ggcagcgcct gtcactattg aactgatacc ggggtatttt cagataacgg tggtcccgaa
     7921 actggctgta tatgacccga cggtgcagtt tgagttctgg ttttcggaaa agcggattgc
     7981 ggatatcagg caggttgaaa ccagcgcccg ctatcttggc acggcgctgt actggatagc
     8041 cgccagtatc aatatcaggc cgggccatga ttattatttt tacgttcgca gtgtgaacac
     8101 cgttggcaaa tcggcatttg tggaggccgt cggtcgggcg agcgatgatg cggaaggtta
     8161 tctgagtttt tataaagggt tgatcaataa aacgcatctc ggcaaggagt tgtggacgca
     8221 gattgataac ggtcagcttg cgccggacct gactgaaatc aggacgtcca ttacgaatgt
     8281 cagcaatgaa atcacgcaaa ccgtcaataa aaaactggaa aatcagagtg ctgcaatcca
     8341 gcagatacag aaagttcagg ttgatacaaa taataacctg aacagcatgt gggctgtgaa
     8401 gctgcaacag atgaaggacg gacgccttta tattgcgggt atcggtgccg gtattgagaa
     8461 tacgccagca ggaatgcaga gtcaggtgct gctggcggca gacaggattg cgatgattaa
     8521 tcctgcgaat ggcaacacaa agccgatgtt tgttggtcag ggcgatcaga tattcatgaa
     8581 cgacgtgttc ctgaaacgcc tgacggctcc gaccattacc agcggcggta atcctccggc
     8641 attttccctg acacctggcg gacggctgac ggcgaaaaat gccgatatca gcggtaacgt
     8701 gaacgcgaac tccgggacgc tcaacaacgt cacgattaat aagaactgtc gggttctggg
     8761 aaaattgtcc gcgaaccaga ttgaaggcga tctcgttaaa acagtgggca aacctttccc
     8821 acgggactcc cgggcaccgg agaggtggcc atcagggacc attaccgtca gggtttatga
     8881 cgatcagccg tttgatcggc aaattgttat tcccgcggtg gcgtttcgcg gtgctaaaca
     8941 tgagcggaag aataacaata tttattcgtc atgccgcctg atagtgaaga aaaacggtgc
     9001 tgaaatttat aaccgaacga ccctggataa tacgctgata tatacgggtg ttattgatat
     9061 gcctgccggt cacggtcaca tgacgctgga gttttctgta tcggcatggc tggtaaatgg
     9121 ctggtatccc acagcaagta tcagcgattt gctggttgtt gtgatgaaga aatccacagc
     9181 aggtatcagt atcagctgaa ttttataacc cagatacggg caccagaaat ggtgcctttt
     9241 ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgctgccatt ttgtccgcag
     9301 ccatctgtct ggctgtatcc ggtgcgcctg catgggcgtc tgaacatcgg tccacgctga
     9361 gcgcggggta tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta
     9421 acgtgaaata ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg
     9481 ccaacgctga agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg
     9541 tgcgtaaccg ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca
     9601 gcgcgtatgc gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc
     9661 tccgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc
     9721 gccacagcaa cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg
     9781 tggccattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca
     9841 tcgtgggtgt cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct
     9901 ggttcaggcg ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag
     9961 atggggcggg taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc
    10021 cgaccgttgt ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga
    10081 ttgaggctga gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc
    10141 gtgtcgggga tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga
    10201 atgcaccaaa ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg
    10261 cgcaggcgca gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg
    10321 tggcggcagc cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg
    10381 atgcaaccgt cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag
    10441 caaatgcagc cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag
    10501 aacaggccaa aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc
    10561 tgtc
//
LOCUS       DAJTNP010000121        10554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTNP010000121 DAJTNP010000000
VERSION     DAJTNP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            597..794
                     /locus_tag="OO160_003476"
     CDS             597..794
                     /locus_tag="OO160_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525461.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO160_003477"
     CDS             complement(744..1937)
                     /locus_tag="OO160_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5525462.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO160_003478"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO160_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5525463.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO160_003479"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO160_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5525464.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO160_003480"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO160_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5525465.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO160_003481"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO160_003481"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5525466.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO160_003482"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO160_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5525467.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2587 a         2679 c         2782 g         2506 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaat
//
LOCUS       DAJTNP010000122        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTNP010000122 DAJTNP010000000
VERSION     DAJTNP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO160_003483"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO160_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5525468.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO160_003484"
     CDS             214..402
                     /locus_tag="OO160_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5525469.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO160_003485"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO160_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5525470.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO160_003486"
     CDS             597..770
                     /locus_tag="OO160_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5525471.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO160_003487"
     CDS             complement(819..1892)
                     /locus_tag="OO160_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5525472.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO160_003488"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO160_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO160_003489"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO160_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5525473.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO160_003490"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO160_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5525474.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO160_003491"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO160_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5525475.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO160_003492"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO160_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO160_003493"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO160_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5525476.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2684 c         2788 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattacagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTNP010000123         9928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTNP010000123 DAJTNP010000000
VERSION     DAJTNP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..339
                     /locus_tag="OO160_003494"
     CDS             <1..339
                     /locus_tag="OO160_003494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525477.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            509..1642
                     /locus_tag="OO160_003495"
     CDS             509..1642
                     /locus_tag="OO160_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525478.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="OO160_003496"
     CDS             2321..2539
                     /locus_tag="OO160_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5525479.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="OO160_003497"
     CDS             2757..2999
                     /locus_tag="OO160_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525480.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO160_003498"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO160_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5525481.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO160_003499"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO160_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5525482.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO160_003500"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO160_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5525483.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO160_003501"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO160_003501"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5525484.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="OO160_003502"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="OO160_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5525485.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2395 c         2543 g         2501 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa atcaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgtaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacacgcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttc
//
LOCUS       DAJTNP010000124         9925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTNP010000124 DAJTNP010000000
VERSION     DAJTNP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1013)
                     /gene="bglB"
                     /locus_tag="OO160_003503"
     CDS             complement(<1..1013)
                     /gene="bglB"
                     /locus_tag="OO160_003503"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5525486.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQI"
     gene            complement(1032..2909)
                     /gene="bglF"
                     /locus_tag="OO160_003504"
     CDS             complement(1032..2909)
                     /gene="bglF"
                     /locus_tag="OO160_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5525487.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3043..3864)
                     /gene="bglG"
                     /locus_tag="OO160_003505"
     CDS             complement(3043..3864)
                     /gene="bglG"
                     /locus_tag="OO160_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5525488.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4151..4876)
                     /gene="phoU"
                     /locus_tag="OO160_003506"
     CDS             complement(4151..4876)
                     /gene="phoU"
                     /locus_tag="OO160_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5525489.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4891..5664)
                     /gene="pstB"
                     /locus_tag="OO160_003507"
     CDS             complement(4891..5664)
                     /gene="pstB"
                     /locus_tag="OO160_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5525490.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5756..6646)
                     /gene="pstA"
                     /locus_tag="OO160_003508"
     CDS             complement(5756..6646)
                     /gene="pstA"
                     /locus_tag="OO160_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5525491.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6646..7605)
                     /gene="pstC"
                     /locus_tag="OO160_003509"
     CDS             complement(6646..7605)
                     /gene="pstC"
                     /locus_tag="OO160_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5525492.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7692..8732)
                     /gene="pstS"
                     /locus_tag="OO160_003510"
     CDS             complement(7692..8732)
                     /gene="pstS"
                     /locus_tag="OO160_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5525493.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8980..>9925)
                     /locus_tag="OO160_003511"
     CDS             complement(8980..>9925)
                     /locus_tag="OO160_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5525494.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2559 a         2582 c         2292 g         2492 t
ORIGIN      
        1 tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat attcagtatg
       61 ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt catgtaataa
      121 ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc agtcatctca
      181 atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg gccacgcgcc
      241 tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa catatcctgt
      301 ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat tttcgcttcg
      361 gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg atgatggatc
      421 gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt gaatggcgcg
      481 tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg ttggtagcgg
      541 gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt agcccaaccg
      601 ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac cagcggcttg
      661 atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc
      721 ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat acgcagacag
      781 gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata aaaatcgatg
      841 gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc cattacgcca
      901 tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc accttccacc
      961 tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt cataattaac
     1021 tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat ctgcgccgtg
     1081 ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg cgtcgtcaga
     1141 tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc gcctgtattg
     1201 accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg
     1261 ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat ggcgtgtaat
     1321 gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc aaccgagggc
     1381 agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc gacgtgaatg
     1441 agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg tggtgtaata
     1501 acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact ggtgatgaaa
     1561 tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc aataacgctg
     1621 gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt gaaaatactt
     1681 ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc ccccaatgct
     1741 ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc atataccgct
     1801 ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac ttttttctgc
     1861 gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat aatggcgggc
     1921 attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat acacagcggc
     1981 accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc cattaccgcg
     2041 cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat catttcgctt
     2101 atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac catcagacat
     2161 agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag tcggcgttcc
     2221 agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga gtaattcaac
     2281 aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg cccgttctcg
     2341 aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc agtaaatgga
     2401 ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa cccaaacaag
     2461 gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt ccactgaaaa
     2521 gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag accgatcaga
     2581 ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc tttctcatca
     2641 ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac cgccaggaag
     2701 acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc caccaccata
     2761 ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc ctttaactta
     2821 aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc cccgactcct
     2881 gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg cggcaaaact
     2941 ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt tcaggttttg
     3001 cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt ctttgcgcac
     3061 gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac gttgatactg
     3121 caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt aattttgttt
     3181 tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa gaatacgcca
     3241 ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt cctggtaatt
     3301 aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg tgacacctgc
     3361 aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa taaagcccac
     3421 ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg cttcttcccc
     3481 tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt tttgctgaaa
     3541 gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt cctgtaattt
     3601 tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca cctcaagagg
     3661 aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca aggcatactc
     3721 tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc caattccgcg
     3781 ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat tattgttgag
     3841 aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa aaaaaccgac
     3901 ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac aatccagtca
     3961 tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat tgcgtgcaga
     4021 gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt aactttgtgt
     4081 aattttagga atttataaag ttatatataa caaatcccaa taattaagtt attgggattt
     4141 gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc tcatcaccgc
     4201 ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa atattctggc
     4261 agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt acgctcggaa
     4321 tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct tcgtattcct
     4381 gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc atccgcgcga
     4441 acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac tccagactta
     4501 ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag attttgtccg
     4561 ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata accagtcgca
     4621 ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca tcgatcgcca
     4681 cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga tcgctgtcct
     4741 ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg cccatggtca
     4801 tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata tgtttattaa
     4861 gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga tgtagtcttc
     4921 tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact caatcaattc
     4981 gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca tgttgtgggt
     5041 gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa tacgcccggt
     5101 agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg ggcgaatggc
     5161 gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt aaccgctctg
     5221 gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc actgcacgcg
     5281 ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg cgatgttgtc
     5341 gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt tcgcacgcag
     5401 cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa tttcaccttc
     5461 cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca gcgtcgattt
     5521 accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat ccaggttgat
     5581 gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct gaattttact
     5641 cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga tgcttttcgc
     5701 taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa aagtgttaac
     5761 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg acgcacaggg
     5821 taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg ctcatcgcaa
     5881 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
     5941 ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg gcaatcgcca
     6001 gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg gagatcatct
     6061 tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
     6121 gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc gcaattacgc
     6181 ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca aacagaccaa
     6241 ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact tctgccagcc
     6301 aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc ggcgtaccga
     6361 ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc agaccaccac
     6421 cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata ccgtcgatac
     6481 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag gccatcgtcg
     6541 ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc attttgcggc
     6601 gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
     6661 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
     6721 aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc
     6781 aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg aggcgctgtc
     6841 gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcggcc
     6901 cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
     6961 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
     7021 ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
     7081 cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg cgccaacaat
     7141 cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga acagcggcgc
     7201 aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca gcagctcaat
     7261 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga acagggcgat
     7321 accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca acgtaccgta
     7381 gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg tccataggaa
     7441 agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga caataatgcc
     7501 acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga aaattatgtc
     7561 gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt actcttcagt
     7621 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc
     7681 ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca
     7741 gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc gttcgcctgt
     7801 ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc ttgttctggt
     7861 ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca tgcatcttcg
     7921 cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca
     7981 ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat cagtttggtg
     8041 tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc acccggcaga
     8101 cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt
     8161 acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg
     8221 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttttgtgaa
     8281 ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg
     8341 cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct
     8401 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc
     8461 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg
     8521 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
     8581 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
     8641 cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt cgcggcgaca
     8701 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
     8761 ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata tataaggaat
     8821 atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa atgtgaagat
     8881 gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa gaaatgggtg
     8941 agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa tctcaacagt
     9001 tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa ggctcgcagg
     9061 agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt tatccatgtc
     9121 tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt aaatttttat
     9181 ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt cggtagcata
     9241 aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact caatagtgac
     9301 tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa aatctgcagc
     9361 tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc aactttgcgg
     9421 agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat ccaccggggt
     9481 gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa tcaattttcc
     9541 tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg atatacttaa
     9601 aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag catgatattc
     9661 aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa catcaaggtt
     9721 cggattaaga tcataataat taatattaga gtgaacccca gcataagtta atgcctggtt
     9781 tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat cacaatgaca
     9841 gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc caaccttatt
     9901 ttcgttcgcg gtaataagat atccc
//
LOCUS       DAJTNP010000125         9888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTNP010000125 DAJTNP010000000
VERSION     DAJTNP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            73..204
                     /locus_tag="OO160_003512"
     CDS             73..204
                     /locus_tag="OO160_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5525495.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            464..1459
                     /gene="pgl"
                     /locus_tag="OO160_003513"
     CDS             464..1459
                     /gene="pgl"
                     /locus_tag="OO160_003513"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5525496.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1500..2453)
                     /locus_tag="OO160_003514"
                     /pseudo
     CDS             complement(1500..2453)
                     /locus_tag="OO160_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2637..3689
                     /locus_tag="OO160_003515"
     CDS             2637..3689
                     /locus_tag="OO160_003515"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5525497.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3765..5198
                     /locus_tag="OO160_003516"
     CDS             3765..5198
                     /locus_tag="OO160_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5525498.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5381..7561
                     /locus_tag="OO160_003517"
     CDS             5381..7561
                     /locus_tag="OO160_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5525499.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7702..8985)
                     /locus_tag="OO160_003518"
     CDS             complement(7702..8985)
                     /locus_tag="OO160_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5525500.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9120..9812)
                     /locus_tag="OO160_003519"
     CDS             complement(9120..9812)
                     /locus_tag="OO160_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5525501.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRA
                     AKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         2398 a         2324 c         2510 g         2656 t
ORIGIN      
        1 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
       61 gggggattga ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
      121 ctacaacgta atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt
      181 tggccagaag ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg
      241 gagagtcata ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg
      301 tgactaatat tttagtaata tgccattcga aatggagtgc taacttattg atttaattga
      361 tgtgcgatat tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta
      421 tatgtatgaa agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta
      481 tatcgccagc cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact
      541 gacgctgaca caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga
      601 caaacgttat ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc
      661 cccggacgat ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca
      721 tatttccacc gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt
      781 gagcgtaacg cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct
      841 ggacggttgc cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt
      901 aaagcaggat cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga
      961 ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa
     1021 cgaacaatat gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa
     1081 agatccgcac ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc
     1141 cgacacccgt tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg
     1201 cgaccgtacc gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag
     1261 taaagaaggc ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg
     1321 caagtatctg attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg
     1381 cgagcagggg ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt
     1441 ggtggttaac gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat
     1501 taacttatct cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct
     1561 ttatcgtcag tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc
     1621 acggcatctt tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg
     1681 ataccaataa tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc
     1741 cccagtcctg cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag
     1801 tccagcttcc agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag
     1861 gaacgtacgc caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg
     1921 gccaatggat ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa
     1981 tcaatggatg aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc
     2041 accgattcca tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac
     2101 agctcattaa atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac
     2161 gctaatgtca ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca
     2221 tcgaatttct ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac
     2281 gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt
     2341 attcttcgcg ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa
     2401 ctggactctg ccagtatgac aaatgccttc atacttgata attcatgctt cattattact
     2461 ccggaaaatg gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag
     2521 ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt
     2581 tgtccttaat gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga
     2641 aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg
     2701 aacatttacc cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg
     2761 gtaacgatct ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca
     2821 ttattagccg ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg
     2881 tccatgagca acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg
     2941 cattcgccat tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc
     3001 gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg
     3061 agtacgaggg tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca
     3121 ctttcctgaa tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg
     3181 attattttga cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc
     3241 cggctgaata tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag
     3301 cattattagc ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg
     3361 atgtcagtaa tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg
     3421 caattaatgt gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg
     3481 ctattgctat ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt
     3541 ctgtaggata tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa
     3601 gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa
     3661 tattttccgg tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa
     3721 taaaggactt ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt
     3781 tatggaagct aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg
     3841 caggattaag cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc
     3901 tggttatcaa gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg
     3961 tggtggtcgg taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact
     4021 cttcaggtac tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca
     4081 ccggtttagg taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg
     4141 gtctgggtta cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca
     4201 ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt
     4261 ccgaaccgga aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg
     4321 tcaccaaaac caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga
     4381 aaatgatcaa cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg
     4441 gattgccggg catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac
     4501 cagaaattaa aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac
     4561 cgatgaaaat ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga
     4621 ttttcagtaa gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca
     4681 tgctgctgct gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata
     4741 ccttaatctg gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt
     4801 tcgaatggtt agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg
     4861 ctttcttcgt tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg
     4921 cctatatcgt tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt
     4981 taatgttaac cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact
     5041 atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt
     5101 ggttggtcgg tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt
     5161 ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt
     5221 tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga
     5281 ttcactcact tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct
     5341 ttctgcgcac ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa
     5401 aggcgtgttt ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat
     5461 taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac
     5521 gtccggaaat atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac
     5581 ctttgttggt attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta
     5641 tgtgctgacc aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga
     5701 ccatgttttt ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat
     5761 tgcggtcatc catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg
     5821 gtcagacagc cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg
     5881 tgagttggta aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc
     5941 ggtgcatctg accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat
     6001 cattggcgcg gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc
     6061 gggcgttagc gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac
     6121 cacctgttta agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca
     6181 cggtcgcggt caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg
     6241 catcagcgtt gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa
     6301 cgtgtatgaa atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat
     6361 tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa
     6421 tggtcgcctg aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa
     6481 cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct
     6541 ggcggtttac ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc
     6601 agatttgatt ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc
     6661 gggcgatacg ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa
     6721 ccgcgaaggc tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg
     6781 ttctatcgct gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg
     6841 ctgggacaac gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca
     6901 gggctttgtg aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg
     6961 gccggaattg ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga
     7021 cgaagtcacc accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa
     7081 tccgattggt ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca
     7141 gattgctggt caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc
     7201 ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg
     7261 cggtctggcg aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa
     7321 ctgggggatg ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat
     7381 ttacatccct ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt
     7441 gatccatgaa gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga
     7501 agagcgcgag attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta
     7561 aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc
     7621 atcttatccg acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaata gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc
     7741 gcggttattg tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc
     7801 gtcgttatca tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc
     7861 ggcccacggt ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg
     7921 accgttggta ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga
     7981 agcattgaaa cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg
     8041 tgcaaacaca tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc
     8101 gaacaccact gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt
     8161 caccagcgta cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac
     8221 aaagaaggtg ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc
     8281 agtacgcagt gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc
     8341 gatggtcaga ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag
     8401 aacaccgata ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt
     8461 acctggcata tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc
     8521 cccatcaagg gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt
     8581 gattccacct ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc
     8641 aatatactgg cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt
     8701 ggtatgcgtt acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag
     8761 ggttatcggg gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg
     8821 cgcatcgaaa ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc
     8881 agtttgatca gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc
     8941 aaaagccaat gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct
     9001 atgaaataga aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa
     9061 aaagcattgc ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat
     9121 tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg
     9181 gtgtccgact tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag
     9241 agtcttgcag acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga
     9301 cctgatgctt ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca
     9361 cgacgagtag atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt
     9421 tccttcattg atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg
     9481 cctgttttgc tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg
     9541 cataaatcac caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa
     9601 catggtgatg attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat
     9661 ctccttacct ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct
     9721 tcagctattg cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc
     9781 gccttgccct cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct
     9841 tcaagtggag catcaggcag ccccctcctt attgctttaa ttttgctc
//
LOCUS       DAJTNP010000126         9710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTNP010000126 DAJTNP010000000
VERSION     DAJTNP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            429..1547
                     /locus_tag="OO160_003520"
     CDS             429..1547
                     /locus_tag="OO160_003520"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5525502.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="OO160_003521"
     CDS             1613..1861
                     /locus_tag="OO160_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5525503.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO160_003522"
     CDS             1926..2294
                     /locus_tag="OO160_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5525504.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO160_003523"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO160_003523"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5525505.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO160_003524"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO160_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5525506.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO160_003525"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO160_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5525507.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO160_003526"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO160_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5525508.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO160_003527"
                     /pseudo
     CDS             complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO160_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2373 a         2691 c         2362 g         2284 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tatggccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 cctatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc taatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 taaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac ggcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattc gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga
     9661 tttgtgattt tagtttggta cgcaatttgg tacacaacac tattttcact
//
LOCUS       DAJTNP010000127         9634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTNP010000127 DAJTNP010000000
VERSION     DAJTNP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO160_003528"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO160_003528"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5525509.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO160_003529"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO160_003529"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5525510.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO160_003530"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO160_003530"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5525511.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO160_003531"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO160_003531"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5525512.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO160_003532"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO160_003532"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5525513.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OO160_003533"
     CDS             5060..6193
                     /locus_tag="OO160_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5525514.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO160_003534"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO160_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5525515.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO160_003535"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO160_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5525516.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO160_003536"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO160_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5525517.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO160_003537"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO160_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5525518.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO160_003538"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO160_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5525519.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OO160_003539"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OO160_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5525520.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2447 a         2472 c         2458 g         2257 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgcgc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aaccactgag
     9541 aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc tgttgttact
     9601 ttttatatag ctaacaccaa taattaacct ttct
//
LOCUS       DAJTNP010000128         9585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTNP010000128 DAJTNP010000000
VERSION     DAJTNP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..316)
                     /gene="psiF"
                     /locus_tag="OO160_003540"
     CDS             complement(<1..316)
                     /gene="psiF"
                     /locus_tag="OO160_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5525521.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKA"
     gene            complement(435..1850)
                     /gene="phoA"
                     /locus_tag="OO160_003541"
     CDS             complement(435..1850)
                     /gene="phoA"
                     /locus_tag="OO160_003541"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5525522.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1951..2211)
                     /gene="iraP"
                     /locus_tag="OO160_003542"
     CDS             complement(1951..2211)
                     /gene="iraP"
                     /locus_tag="OO160_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5525523.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2393..2596)
                     /locus_tag="OO160_003543"
     CDS             complement(2393..2596)
                     /locus_tag="OO160_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525524.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2674..3768
                     /gene="ddlA"
                     /locus_tag="OO160_003544"
     CDS             2674..3768
                     /gene="ddlA"
                     /locus_tag="OO160_003544"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5525525.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3792..4004)
                     /locus_tag="OO160_003545"
     CDS             complement(3792..4004)
                     /locus_tag="OO160_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5525526.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4264..4572
                     /locus_tag="OO160_003546"
     CDS             4264..4572
                     /locus_tag="OO160_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5525527.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4631..5725)
                     /gene="yaiW"
                     /locus_tag="OO160_003547"
     CDS             complement(4631..5725)
                     /gene="yaiW"
                     /locus_tag="OO160_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5525528.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5738..6958)
                     /gene="sbmA"
                     /locus_tag="OO160_003548"
     CDS             complement(5738..6958)
                     /gene="sbmA"
                     /locus_tag="OO160_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5525529.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7310..8467
                     /gene="ampH"
                     /locus_tag="OO160_003549"
     CDS             7310..8467
                     /gene="ampH"
                     /locus_tag="OO160_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5525530.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8468..8971)
                     /locus_tag="OO160_003550"
     CDS             complement(8468..8971)
                     /locus_tag="OO160_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5525531.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9059..>9585)
                     /locus_tag="OO160_003551"
     CDS             complement(9059..>9585)
                     /locus_tag="OO160_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5525532.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2378 a         2447 c         2281 g         2479 t
ORIGIN      
        1 cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc agagcttgtt
       61 gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa cttttttcac
      121 caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt
      181 tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgtggggtta
      241 aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc aaggtaacca
      301 gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg tttgcgcccg
      361 tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac
      421 tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa gagatcggtc
      481 tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat acgcaactga
      541 ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac catcactgcg
      601 ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg cgcaacaacc
      661 tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc ctttttagcg
      721 aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa
      781 gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag gaaaaatcct
      841 ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag ggttggtaca
      901 ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat attgccgtgg
      961 tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa caggccaagc
     1021 aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc gttcaccaac
     1081 tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc accagcggtt
     1141 gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg agcgttaagc
     1201 agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg acatttttca
     1261 ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag cgcagcgggc
     1321 gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc ggcttttgcc
     1381 atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc gccgttatag
     1441 gttttgacat cggttgacca ggcggttgct gatgcagccg agtcggtgac gtagtccggt
     1501 ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg taaggcatcg
     1561 atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt aatttccgaa
     1621 tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc gctaagagaa
     1681 tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg tgcagtaata
     1741 tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc ttttgtcaca
     1801 ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac tttattttct
     1861 ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg ccgtgacaac
     1921 tttatgacag ctgttgaaaa gattaacttt ttactgacaa ggatgcttca ataacttctt
     1981 tacgtaatcg cgcagcagct ctgtatcgtc gtcaggaatg ctggcatcgg gctttacctc
     2041 gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg ccatattgcg
     2101 aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt ctttcgactc
     2161 ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca tgtctgtatt
     2221 tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag tgtctggaag
     2281 tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga ttgccagcga
     2341 aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat tatcaacctt
     2401 tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta agagaaaaaa
     2461 actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga
     2521 tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag
     2581 cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
     2641 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
     2701 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
     2761 gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg
     2821 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg
     2881 cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
     2941 gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
     3001 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt
     3061 tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc
     3121 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc
     3181 agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag
     3241 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat
     3301 ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc
     3361 gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc
     3421 accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     3481 gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct
     3541 tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac
     3601 gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg
     3661 aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg
     3721 gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc
     3781 cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaacccc
     3841 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     3901 gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     3961 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     4021 aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta
     4081 attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt
     4141 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     4201 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     4261 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     4321 tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg
     4381 caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag
     4441 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc
     4501 ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat
     4561 cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct ggcctgacat
     4621 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     4681 aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     4741 cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     4801 ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc
     4861 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac
     4921 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg
     4981 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt
     5041 atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     5101 cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt
     5161 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat
     5221 taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg
     5281 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg
     5341 gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac
     5401 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     5461 aatttgactc ctgctgcgcc accgccagca ccgagtagac gttttccagc gtcggtgcca
     5521 gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     5581 tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca
     5641 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     5701 aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     5761 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     5821 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     5881 caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg
     5941 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt
     6001 tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct
     6061 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac
     6121 ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca
     6181 ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     6241 gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca
     6301 ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg
     6361 aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt
     6421 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac
     6481 tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     6541 cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt
     6601 gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact tccaccaaaa
     6661 accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg
     6721 ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     6781 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     6841 cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg
     6901 cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat
     6961 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     7021 caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     7081 gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg
     7141 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     7201 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     7261 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     7321 tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc
     7381 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     7441 cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     7501 tagttatggt gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg
     7561 tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg
     7621 gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac
     7681 ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg
     7741 tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg
     7801 ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc
     7861 taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac
     7921 ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca cctacacccc
     7981 ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa
     8041 cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg
     8101 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca
     8161 gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa
     8221 agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc gtccgaggat
     8281 tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     8341 gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag
     8401 cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc
     8461 atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     8521 cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc
     8581 gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt
     8641 caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat cccgtacaat
     8701 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca
     8761 taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa
     8821 ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct
     8881 tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa
     8941 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     9001 aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt
     9061 accaagtata tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga
     9121 ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac
     9181 caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat
     9241 cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt
     9301 cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag
     9361 gctgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac
     9421 cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg
     9481 ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc
     9541 tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagc
//
LOCUS       DAJTNP010000129         9538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTNP010000129 DAJTNP010000000
VERSION     DAJTNP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(120..813)
                     /gene="ospZ"
                     /locus_tag="OO160_003552"
                     /pseudo
     CDS             complement(120..813)
                     /gene="ospZ"
                     /locus_tag="OO160_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            869..1021
                     /locus_tag="OO160_003553"
                     /pseudo
     CDS             869..1021
                     /locus_tag="OO160_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2402..3349
                     /gene="icsP"
                     /locus_tag="OO160_003554"
     CDS             2402..3349
                     /gene="icsP"
                     /locus_tag="OO160_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5525533.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3911..4283)
                     /locus_tag="OO160_003555"
                     /pseudo
     CDS             complement(3911..4283)
                     /locus_tag="OO160_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4527..4646
                     /locus_tag="OO160_003556"
                     /pseudo
     CDS             4527..4646
                     /locus_tag="OO160_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4896..5762
                     /gene="ospB"
                     /locus_tag="OO160_003557"
     CDS             4896..5762
                     /gene="ospB"
                     /locus_tag="OO160_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5525534.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            6091..6831
                     /gene="phoN2"
                     /locus_tag="OO160_003558"
     CDS             6091..6831
                     /gene="phoN2"
                     /locus_tag="OO160_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5525535.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7184..8638)
                     /gene="ospC4"
                     /locus_tag="OO160_003559"
                     /pseudo
     CDS             complement(7184..8638)
                     /gene="ospC4"
                     /locus_tag="OO160_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
     gene            complement(9005..9538)
                     /locus_tag="OO160_003560"
                     /pseudo
     CDS             complement(9005..9538)
                     /locus_tag="OO160_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2947 a         1790 c         1823 g         2978 t
ORIGIN      
        1 gcactaagac cgcacgaata taacggtggg ttacccccaa acgaatcgga aaatcgatac
       61 tggaaaaact ctaactcggt ggccagtttt tgttgaccac ttcatacaat taatatacat
      121 taagtaacag gcattcgagc cttgatttga gaaagcctgt cattcatttg ttttatgtag
      181 ctatctattt cctctgatgc ataactaaaa agttctccca tttcacgagc aataataatc
      241 tcagatttaa tagactttaa tctctggcga ctcatctctt aacacttgca taatctctct
      301 ttttatatca tatagttcta taaaccgatt tgcccgtagt tctccttggg aagaatcatt
      361 tctgaataca acaattatat acaaaaaatc actattttct ttagagaaga tcttacaact
      421 aatctctggg agctcacata atgcatgtaa gtcaggatgg tacaaactat ccacagaaaa
      481 ttgccccata ggacgttttc cagatataac tgtcaaccag cttccttccg gtgtactggc
      541 ttcggctctg gaaataggct gaaaatcttc cggacatgga gaatacattg tcttcatata
      601 attaagtcgt ctggatttct cattaataaa ctcctctatt tttaattgtg ccatacttct
      661 ggttatacca gagatatact tcttgtcata tatgtttggt gattcattaa agtaaccatg
      721 ttcaactcta ggataaatat ttctcactat atattcagta tttgcagtat ttattggaaa
      781 tacgttctta atattcttga tgggactaat cattttaatc tctatactca aatataaaca
      841 ttaccatgaa caatataatt gttcctaagt ggaatgtctc cacggagaac actttatcta
      901 tcgggaaata gtgcgggcaa cagtgtttaa ttatatcgag tgcaattaca atcgctggcg
      961 acggcaccgt tggtgtggcg gcctcagtcc ggaacaattt gaaaaccaga acctcgctta
     1021 ggcctgtgtc catattacgt gggtaggctc acagagcctc acttacgact gcgtccatat
     1081 tatgtgggta ggatcaataa agacgtttta tatgtgtctg cttgcagcca gaaaatttat
     1141 accctgcagg tatcataaac atgcttgata acttaattgg ggctccctct tccttttatg
     1201 gtgtaattgt ttgattgaat acttccgggg atttcagtat gaaatgaagt atatttaata
     1261 tactttacaa aaagtttgta ttagtggaat aaggacaata gcttatccaa actgccaagc
     1321 ctctttatta taagtaagat ctggcactgg aatattctca aaaggactaa catcatcacc
     1381 acgaccttca accaaccatt ttattgcatt taaatcccag aatagttcag tagtggtcaa
     1441 tttgggaaac gcacaatatt gtacggttac gttatcgcaa tagagtgaac tctcgcccta
     1501 tacggcgttt tcaaggatta gtccttaatc ggacaaccac attaaagcat aataaataat
     1561 taagctttaa taatatacta tagatacagt tctattataa aaaatactat tttaggtgat
     1621 gcattaaatt agtagaatat ttattctttt ataaaagcac caatgaattg tccaagtaaa
     1681 catgagataa aatgtgtaga ggataactga gtttggtcac tttaactgta ttagtcgcag
     1741 tctgactctc gactttaaaa ggatgggaat tacctgtact gatagtgaga aagctttcca
     1801 ttgaaataag tcaaataaaa gttgatggct tcagtgctaa agtgaggtgc ccgctagccg
     1861 tattcactcg aatcggaatt caatgtattg cttctgccat ttccataata tttcacggga
     1921 aaaacaaaat ctgaaaaact atacttaagt gttgtattga gtcagttgag atacaaatac
     1981 atatttatca gcacacgtta cgtcaaatac aggaggttgt catgttggta ctgaaaggca
     2041 cgttggctat tgcagccgtg atgacaattt attgtcttat cgttgttctt atggaacgct
     2101 tttctgattg atttcatatt ggcgagagaa caagaaggtg agtagaattg tcgcggaatc
     2161 ctgaaactat cagcctcagg tatgaacacc aaaaggtagg tagagaaatc tatggccccc
     2221 gttactttat tcgcacaatg aggtattttt ttatttttac aaaagttcaa taaatattat
     2281 aataaaagat atgttcttgg taacataaac aaatatttaa ttaaaataat gcttggcatt
     2341 gacgtttatc ttataaagta agaagatcat ggacatttca accaaaaaag tagagttctc
     2401 gatgaaatta aaattctttg tacttgcact ttgtgtacct gcgatcttta ctacacatgc
     2461 taccactaac tatccacttt tcataccgga caacatcagt acggatatta gtctaggatc
     2521 tctgagtggc aaaacaaaag aacgcgttta tcatcccaag gaagggggga gaaaaattag
     2581 tcaactggac tggaaataca gtaatgcgac tattgttaga ggtggcatcg attggaagct
     2641 aattccaaaa gtgtctttcg gagtttccgg ttggactact ttaggtaacc agaaagcaag
     2701 catggttgat aaagactgga acaattccaa tacccctcag gtatggaccg accagagctg
     2761 gcatcccaat acgcatctcc gtgatgctaa cgaattcgag ctgaatctta aaggttggtt
     2821 attaaataat ttggattatc gacttggact aatagcaggt taccaagaga gtcgttacag
     2881 ttttaatgca atgggaggga gttatattta tagtgaaaac ggggggagca ggaataaaaa
     2941 aggggcacat cctagtggtg aaagaacaat aggttacaaa cagctcttca aaatacctta
     3001 tattggatta actgctaatt accgccatga gaattttgag tttggagcag aactgaaata
     3061 tagtggttgg gttctttcat ctgatacaga taaacactat cagactgaga caatttttaa
     3121 agatgaaata aaaaaccaaa attactgctc tgttgctgcg aatattggat actatgtcac
     3181 ccccagtgca aaattttata tagaaggctc cagaaattac atttctaata aaaaaggtga
     3241 tacatctctt tatgagcaaa gtaccaatat atctggcacc attaaaaata gtgcaagtat
     3301 tgaatatatt ggttttctca cttccgcagg tataaagtat attttttgaa ttggttcacc
     3361 gagttgatta cgttttcatg gctaaaaatt gccgcccatt gtgagtcata ttcgccctga
     3421 ctttccagaa ccatacgaac tgcccgttga cggacttcgg gggaaaaacg tgtattttta
     3481 gtcattctgt ttacctcttt ctcagggcgt ttagtctcca ggatttccgg ggcggttcaa
     3541 aaaactcttc gttgattact gtggacttac tgttggcatt accgaccctg agaccggaga
     3601 aataagaacc gcttagatca tcgtggctgt tctcggggta tcaagttaca catgggcaga
     3661 ggctacccaa ggtctactat tatcgcctga aagcgctgat ggaacagtgc tatcagggac
     3721 atgctgatga aaatacctaa ataatcagcc agcactctat ctttccaaat ccacagcata
     3781 gcaaagagag caaaagagcc tgtaaattca gaaattaagg aaacgcctct tggtgtacat
     3841 aatctatctt atgttaaatg aaatctaatt tcttgattat atgctggatc gttcgtgggt
     3901 ggctttgccc ctatatttcc agacacctgt tatcattggc ccgctgccgc agatattccc
     3961 gtggcgagcg ataatccagc gcactatgcg gatgccattc gttataatgc tcgaatgcct
     4021 ctgcaaggtt ctttgctgcc gttaacccgt caggtttggg catgatactg atgtagtcac
     4081 actttattgt tttcacgaag ctctttgcta ttctgttact ctccggactc cgcaccgccg
     4141 tgttcttcgg ttcaacaaag tcccaacatc cgggtgaact ggcgtgtttc attagctcgg
     4201 tagcatgaac cattatccgt cagtcacttc actggagacg ccggaagctc gttgccgaag
     4261 cggtgttcca ccgctcccag catgtcgcca acgggagagc atctacggtc tgattgaact
     4321 gtttattcaa attgtccacc gtctgtcagt acgctctgaa cgtcggctgg ttaaaacact
     4381 gctcgctgat tttcagaggg ttcacggtaa aacagcactt ttattccgga ttgcggaagc
     4441 ggctctgaat aatccggatg ggctggttaa agaggttgtg taccatctgc atattgttga
     4501 accagatcgc agcggtaaga gaagttcatc atacttggcg cagttacgcg atgtttctgc
     4561 tagaggtgat gccgtaaaga acggcagaac attaccggaa caagatagcg gtctacctgc
     4621 actggtatca gacccggggc ttccccggat gatctccacg gtactttagg tactgtgtct
     4681 atattatgta ggtaggatca attaactttg tagttgaaat tctttcattg aaataatgaa
     4741 taaaaaagac aaaccgcatt aaacaatgcc agaatataat tgcatttttg ttccgttttt
     4801 ttaaatcata gtatacaaag aagcttgcat actatatgaa aatataacac cctttactat
     4861 gatgtttata aaacaatata tggagtcatg taggtatgaa tttagatggt gttagaccat
     4921 actgtagaat agtcaataaa aagaatgaaa gcatatcaga tattgcattt gcacatataa
     4981 taaaaagggt aaaaaattca tcatgtactc acccaaaagc agcattggtt tttttaggag
     5041 agaaaggttt ttgtgatagc aatgatgttc tatctattat gggacaacaa ataccaagag
     5101 tatttaagaa caagatgtta tatgattatg tttttaaaaa tgaaaaaagt aaaaatgatt
     5161 ttctaaaaat ggctgaatca tggctaccac agagtgaacc aatagtaata aataatgatg
     5221 atgacgcatt gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa
     5281 acgatactga ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt
     5341 ctcatcaatt aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga
     5401 aagactgtgg tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata
     5461 aagtggctcc taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact
     5521 ctctgccttt ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa
     5581 acgatgagtc attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact
     5641 atggtcaaga gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg
     5701 agcatacagt aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt
     5761 agtataaaat ttttaaccta tagttaattg tttgcattta agatgaagtt attttatctt
     5821 tagtagaaaa gtgcttccct atgagggaaa atcacaaatc atcataatac agagacaaaa
     5881 cgatacgaaa aaatagataa aaaacatcat tcttttacca cattattttc cgtgaatatg
     5941 aaaaataatg ttattacttt aatataagac tattttttgt ttttccatca ctctgttcaa
     6001 atttttccgc atgacttgtg ttttttgtaa tacagctcgt tttttacagc tgaccaaaat
     6061 catcaattaa ttatgctaag gaaataaatt atgaaaacca aaaactttct tcttttttgt
     6121 attgctacaa atatgatttt tatcccctca gcaaatgctc tgaaggcaga aggttttctc
     6181 actcaacaaa cttcaccaga cagtttgtca atacttccgc cgcctccggc agagaattca
     6241 gtagtatttc aggctgacaa agctcattat gaattcggcc gctcgctccg ggatgctaat
     6301 cgtgtacgtc tcgctagcga agatgcatac tacgagaatt ttggtcttgc attttcagat
     6361 gcttatggca tggatatttc aagggaaaat accccaatct tatatcagtt gttaacacaa
     6421 gtactacagg atagccatga ttacgccgtg cgtaacgcca aagaatatta taaaagagtt
     6481 cgtccattcg ttatttataa agacgcaacc tgtacacctg ataaagatga gaaaatggct
     6541 atcactggct cttatccctc tggtcatgca tcctttggtt gggcagtagc actgatactt
     6601 gcggagatta atcctcaacg taaagcggaa atacttcgac gtggatatga gtttggagaa
     6661 agtcgggtca tctgcggtgc gcattggcaa agcgatgtag aggctgggcg tttaatggga
     6721 gcatcggttg ttgcagtact tcataataca cctgaattta ccaaaagcct tagcgaagcc
     6781 aaaaaagagt ttgaagaatt aaatactcct accaatgaac tgaccccata aagctggaca
     6841 gcctgtatca ggctatggag ggcccataga caaatctacc ctatatgagc ataggaggag
     6901 tctatgggca caccacgttt tacccctgaa tttaagggat tactggaaag gctgggacat
     6961 atccctccgg cagaagcaga aaaagcttat tatgctgcca tcggaaacga tgatctggca
     7021 acctgagttc acagataaaa cattctctag gaaactcggg gcggttccgt tcaccacatg
     7081 caatgtggtg ttgcagggga acggtctgca ccatccccta tgtcgatcaa tataacagaa
     7141 atgacaactt cagattcagg gcacaaccta aatatatttt aggtcacctc tcaaatcgtt
     7201 tgcctgatac cgctccattt tttaataaaa aaatcaatca tttttgaatc tttgcttttc
     7261 attgcattgt caagcatagt gtccccacta tttgcttttt gaaatttttt gtttacatca
     7321 accaatccat tattgataaa atattctagc actttatagt tggcaccatc tttactaagt
     7381 aagtattcaa catcatacag attacaatat ctattgtttt tcattttctc catctctgcg
     7441 acatcttgtt ttgtaaaatt aaatttagat agtaaataaa gtgctatatc agctttttta
     7501 ttgcctaaag aataagcaag ggcctgatga gccatttttt tatcagtcac tttattgtta
     7561 atctcttcat aattagatgc tttaatcgca tcctccaaag atatgtctct gagttttatt
     7621 tttttgaagt tatctgtact tagcattctt ggtatatgat actcaagctg aaacacaaag
     7681 tttataattc tatccagact aacaggatca atatttttgt tataacaaaa ctctctgaat
     7741 ccttgatctt tactctttct tataaaatca ataagatgca acccaattcc aagcctcata
     7801 tctttagtgt taaatattgg aacatcagtt ttcccctgat taccatgcac atacctgcta
     7861 acttcatcca caacctcttt aaagttatct aagaaaaatg attggtgctc atggtaaaaa
     7921 acaggtggaa tagcattatc aaagtgatcg gttaatgtca tatatccata tggagagtca
     7981 tgatcaatga tatacgcatt ggcgccaaaa tctacagtat gatgtgtttt atttagtggg
     8041 agtttttctt gatgattgga aatttcgacc ccaaaaaaaa caaaatcgtg gttagagatt
     8101 tctttaatat cctcagagta tgtattctta tgtgtttgta tattttcaga ctgaatttta
     8161 tcaaaggatt taatattaag aatattattt gacagattag agttactttg atgtctgaag
     8221 ttaaaattag aattaataat aatagaaagt aagtgacgtt cgtctgaaga aagaggatga
     8281 actttttgta ttatctcaga gatatagtgc ctggacacct tatcgagatg ctcaagtgac
     8341 cgaaatgatg accgtgctcc agatggcgct tcaagagaaa gatttagtga tttgaaactg
     8401 gtcccttgag aaaacatttt gctataactt tgcgctgcta tagacttcct taaaaagttt
     8461 ttcacagtat tagcacagtg atctaaatgt ttttcactta tgccagaaga agatgagttt
     8521 gttacgcgta atatattttt tgtaatgctt ttgttgcttt attcggattc gtttttccat
     8581 caacaagatc aatattattc tgaacattaa tatgatttac tgcttcaggt attttcataa
     8641 aatattcccc attatgtgcg tttttatcta tggttattta gaatatatta tctaacttaa
     8701 tatttccttg tgtgtattat aacgaaatga acatatttta atatgttgta caatttttac
     8761 tctgaaatcc cctgaaatta atcacacctc ctttactaaa aggaattatt gcaacggtat
     8821 tatttgtttt tgtatatata tcttgcgcct tgttatgtag ttaatcctca gaatatgata
     8881 gataaaatag agacacagag cagagcgtgt tctaacatca tgataacata aaataattag
     8941 taacaataaa tgaaagaatc atgggagctc aacaaaaaga gagctaaata gctgtgtgga
     9001 atagtagatc actttggcgg tataatggca gatggtctgc atactttccg gtaacaacat
     9061 gggccagaag ccccgctccg gcataactgc gtgcaatggg ttttgaaggc accggtgcct
     9121 gcacgatatg gtcgcaccgg cgacaggcca gtttagggcg ttgtttttcg ataaccttaa
     9181 aggcgctgct gataagctcc agctgctctg acacatcaca tcccagtgga ctgagttcac
     9241 caccacaggc cggacagcat tcctcttccg gccggatgac ccggggggca cggggaagtg
     9301 aggctggtaa cggtttacgg gctgaagact ggcgcaggga agatggcagt accgggtcat
     9361 attgctcacc cagtgtttcc gccatttcct cctgaagtgc gctgattcgc tcctgtgctt
     9421 cctgtatcca ccgttcggtt tttgcgcgaa gtttttctga gcttttaccg aactgcatac
     9481 gctgcagttt cgcgaccagc gccttcagtc ggttgatttc ggaaggcctg gacaactg
//
LOCUS       DAJTNP010000130         9506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTNP010000130 DAJTNP010000000
VERSION     DAJTNP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..210)
                     /locus_tag="OO160_003561"
     CDS             complement(<1..210)
                     /locus_tag="OO160_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525536.1"
                     /translation="MCYEYDAYYLYRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGV
                     VCDPYICVNSDGISPELTRKYLGEKA"
     gene            723..1418
                     /gene="minC"
                     /locus_tag="OO160_003562"
     CDS             723..1418
                     /gene="minC"
                     /locus_tag="OO160_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5525537.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1442..2254
                     /gene="minD"
                     /locus_tag="OO160_003563"
     CDS             1442..2254
                     /gene="minD"
                     /locus_tag="OO160_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5525538.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2258..2524
                     /gene="minE"
                     /locus_tag="OO160_003564"
     CDS             2258..2524
                     /gene="minE"
                     /locus_tag="OO160_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5525539.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2896..3240)
                     /locus_tag="OO160_003565"
                     /pseudo
     CDS             complement(2896..3240)
                     /locus_tag="OO160_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3275..3394)
                     /locus_tag="OO160_003566"
     CDS             complement(3275..3394)
                     /locus_tag="OO160_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5525540.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3355..3540)
                     /locus_tag="OO160_003567"
     CDS             complement(3355..3540)
                     /locus_tag="OO160_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525541.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3641..3814
                     /locus_tag="OO160_003568"
     CDS             3641..3814
                     /locus_tag="OO160_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525542.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3816..4160
                     /locus_tag="OO160_003569"
     CDS             3816..4160
                     /locus_tag="OO160_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5525543.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4164..4499
                     /locus_tag="OO160_003570"
     CDS             4164..4499
                     /locus_tag="OO160_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5525544.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4556..6877)
                     /locus_tag="OO160_003571"
     CDS             complement(4556..6877)
                     /locus_tag="OO160_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5525545.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6994..7203)
                     /locus_tag="OO160_003572"
     CDS             complement(6994..7203)
                     /locus_tag="OO160_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525546.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7954..9477)
                     /locus_tag="OO160_003573"
     CDS             complement(7954..9477)
                     /locus_tag="OO160_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5525547.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2665 a         2207 c         2081 g         2553 t
ORIGIN      
        1 ggctttttcg ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca
       61 aatatacggg tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc
      121 agacgccatt gatgagaaca tcatccccac acctgacaat ccgatataaa taatccgata
      181 taaataatac gcatcatatt catagcacat cctgataatc caaccgtaca acacttatgc
      241 tcatttcgtc atcaatccgt aatcaaaacc gctgacctgg acgagttacg atgacctgac
      301 agaagtatca gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag
      361 ataacgggta tctgttcttt aatgatgctg caaaaaatgg aaagttaaga aagcctggcg
      421 tggtgaaaaa atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca
      481 cgattagggt gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg
      541 gttgattgac aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact
      601 tttactctga ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct
      661 tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca
      721 ggatgtcaaa cactccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc
      781 tgcatgaggc agaacctaag gttatccatc aggcgctgaa agacaaaatc gctcaggccc
      841 ccacattttt aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa
      901 actggtcagc gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg
      961 gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg
     1021 aaggaaaaga aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa
     1081 tgccggtcac aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg
     1141 ctccacaatg tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg
     1201 atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg
     1261 accgggaaac gcaaatattt tgtacgaacc tgatggcgga attggtgtcc atcgcaggtg
     1321 aatactggct gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt
     1381 tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc
     1441 tatggcacgc attattgttg ttacttcggg taaagggggt gttggtaaga caacctccag
     1501 cgcggccatc gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga
     1561 tatcggcctg cgtaatctcg acctgattat gggttgcgaa cgccgggtcg tttacgattt
     1621 cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac
     1681 tgaaaatctc tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga
     1741 aggggtcgcc aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga
     1801 ctccccggca gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat
     1861 tattaccacc aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct
     1921 ggcgtcaaaa tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt
     1981 aacgcgctat aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct
     2041 ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc
     2101 ctctaaccag ggtgaaccgg tcattctcga cattaacgcc gatgcgggta aagcctacgc
     2161 agataccgta gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa
     2221 gaaaggcttc ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct
     2281 ctcgcggaag aagaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga
     2341 acgccgtcgc agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga
     2401 ggtcatttgt aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa
     2461 agatggcgat atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa
     2521 ataagcccgc tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt
     2581 tcatccctct catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg
     2641 ctatttccac ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta
     2701 tgcctggttg agtaaataac catgttatta gtgtgtatat actgggagat gataaccccg
     2761 caatttcatt attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt
     2821 tttttgcccc agaattttct ctttcaacat cctgtaaacg aaaactgcgc cgaagcgcag
     2881 tttaatcgtt gatggtcaga acgaccagtt cacaccagcc accgcgttcc acggggactc
     2941 cacaccggca ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga
     3001 ggctttaata cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc
     3061 gtcactattc actttcaccc gattatcatc gacaaattct ttgcgcacag ccgccttcag
     3121 gcagggttca atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag
     3181 ttcacgatat atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata
     3241 gtagcgtcta agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg
     3301 attcatcctg aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga
     3361 tgaaatgatg atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt
     3421 agttggataa aaaatcactc ttttcagata atactgtcgt acgcttatct ttagacaatg
     3481 aggccgtcgg aaaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac
     3541 ataaaaatca cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta
     3601 acagtcaatg acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa
     3661 gattattctc attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa
     3721 ttatctgaat aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat
     3781 tgtgtgtgca acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg
     3841 ggcttatctc agcattgttc tgctccacgc cagcgatggc ggatatgaac cgcaccacga
     3901 aaggtgcatt gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac
     3961 tgaaaggtgc agctgttggc gctggtgtta gtgcagtaac agaaaaaggc cgcgacggta
     4021 aaaatgcgcg taaaggtgca aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga
     4081 ccggcaatgg cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa
     4141 ttctgggtaa aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt
     4201 ttgttttcgc accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag
     4261 aaacctatat tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg
     4321 cagaaattgt tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc
     4381 aatctccgga actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc
     4441 cgcaaatgct gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat
     4501 atttcatatg taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga
     4561 acttatattt caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca
     4621 cgcccacatt cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa
     4681 tttcccccaa attactcacc ccatcttgtt cgactttcac accgttcatt gagacagcaa
     4741 aatctttact gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg
     4801 gaccgctgtt tttctcctga gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt
     4861 tgttatttgc gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga
     4921 cgcccatcca gatagcctgt gcctgtggct ggaagatcca cgtatatttc gccgcttcca
     4981 gaccaaagga tttattcagg gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt
     5041 tcgcgctata ggattcatag gctaattcat cccctttcac ggagttttta aaccagctat
     5101 attgcgccca ggagtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg
     5161 catacagccc tgcgctatag cctctgacac tccctttcga acgataatcc gacacgctgg
     5221 aatgagttaa gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac
     5281 tatcggtaaa accaccggtt aacagatcgc cccctaactg cgagacgtag cgatgcgagg
     5341 tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc
     5401 aaagtcggct tgaacgctcc tgttcagtta caggatcgat gtagcgcgtt tcacccgcac
     5461 ggtcgttcag acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc
     5521 cagcttcggg gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt
     5581 ttgtttcacc gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt
     5641 cggtattacc tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa
     5701 taaattcccc atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag
     5761 ctccgctgcc tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa
     5821 taacgacttt atccgtagct gaattatcat catccaattc agttctgaga tgcaatacgc
     5881 cattattacc aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat
     5941 ctgcccggga aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag
     6001 ctttaatatc ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttatcatctg
     6061 ttgaaatatt agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt
     6121 taatattatt gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt
     6181 tactcgtctc atctttatgg tagaggattt catttgctga ccgtagtgag ctaccagata
     6241 aaatatctac cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct
     6301 gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcaa
     6361 tcgcggcgtc aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc
     6421 gggcagcgat ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa
     6481 cgctggcatt ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg
     6541 cggcataact ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta
     6601 tggcattatt ttcattactt cctgtatcgg cagtactttc ttcatcaaga gaagatacac
     6661 acgcacacgc gcttccggga gatgcacacg caagaacgcc gactgcgtga ttacccgcag
     6721 taacaataaa tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga
     6781 ctcttattat ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt
     6841 aatcccccgt tgtgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac
     6901 ttacgattgc gctagctgtt aatgctgggc ttagaggact attgtcagaa ggatataaac
     6961 cgcccgttgt tacaatgacg ccattgttaa tggttaactt actaccctct cctgcaacgg
     7021 atacccgtaa cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt
     7081 tccatctttt attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata
     7141 tgcattaccc accgccagag caataagtcc caaactaaac ccggggagtt ttttcagttt
     7201 catagattaa ctatccattc attggattaa caaacattcc tggtcaaata acctatgagc
     7261 tcagattacc aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata
     7321 aacagcgaca atatataaat gataaataag tcggcattat catttttcac cttattttca
     7381 tgacattgat cactttgggg tgactatttt gtttaacaaa taactcttcg acatcaaaat
     7441 tattaatagt tgccatggta attatttcgg tgatatataa aaacaactgt gataacacca
     7501 aggttgacaa ctatttgcgc aataattcct ttccttcaat gatccaggcg ttcactgcca
     7561 gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg
     7621 gctaacgagt ctttctctta aatcgattaa ccagattgat cgccagccaa acaagggcat
     7681 taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga
     7741 tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat
     7801 ccagattatt actattattc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt
     7861 atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta gctgtctata
     7921 attacaccgc actatggcct tactgatcaa ttttcactca accacaacct tcaccttcgg
     7981 tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga aataataacc
     8041 ctgctgaaat gtgatattat tttggttcag atagccaagt tgttctttcg tttcgacacc
     8101 ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg
     8161 ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt tgatataatc
     8221 aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat catccagcgc
     8281 gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt
     8341 caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc
     8401 acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt
     8461 aattccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac
     8521 ctgattcatc aggctttgtg taagcggaac gattaatccg gatttttcag caagtggaat
     8581 aaatgccgcg ggtgaaatat atccaccgtg aggttgtttc cagcgggcta acacctcaac
     8641 tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga cgatctcccc
     8701 attatttatt gcccgtcgca ggatctcttc aggcgttgcg cttttattga aatatctatc
     8761 aagcaggaag gcagcggcgc tggcgattaa cagaataaat attaaaatcc caaagccttt
     8821 atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag tgaaatgata
     8881 ttttgtggag tgaaccttct cggtataggg ataatgtcca ttaagcgtca tcacatcagc
     8941 cgttggtcca ataatgtcat ccgccacgcg taatacatac cttacccctt tcaagggtac
     9001 attaagcgcc ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg tatctgcaaa
     9061 ttggctctga tagagcagga taggtaatct attaacggtg tcgattgccg gagccaacag
     9121 taaattactg tccgggacaa caggaatacg agacagcagg acccgattcc caggcaggga
     9181 tgtacaccag actattccct gtttgagaat gatgattgtg cgcaggtgag gtttcagagc
     9241 tgcttcagtg ccaagctgat attgctcctc gaggtcgcat tccttcccgg caatgttaac
     9301 cgctgtttga gtcgcctgtg acgcttcttc aagtatatga ttgatattgt tggcggcata
     9361 tcttgctccc gccaggtact ctgcttttgc ggaataccag agctggatgt tcaaaatagc
     9421 gacaccggta ataaacaagc atatcgcaac gaggatgggt atgagtgtat tgcgcatttg
     9481 cttgtcccgg ccaaagggtt taatac
//
LOCUS       DAJTNP010000131         9424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTNP010000131 DAJTNP010000000
VERSION     DAJTNP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..311)
                     /locus_tag="OO160_003574"
     CDS             complement(<1..311)
                     /locus_tag="OO160_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5525548.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
     gene            complement(492..1868)
                     /gene="trkA"
                     /locus_tag="OO160_003575"
     CDS             complement(492..1868)
                     /gene="trkA"
                     /locus_tag="OO160_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5525549.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1890..3179)
                     /gene="rsmB"
                     /locus_tag="OO160_003576"
     CDS             complement(1890..3179)
                     /gene="rsmB"
                     /locus_tag="OO160_003576"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5525550.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(3225..4172)
                     /gene="fmt"
                     /locus_tag="OO160_003577"
     CDS             complement(3225..4172)
                     /gene="fmt"
                     /locus_tag="OO160_003577"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5525551.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(4187..4696)
                     /gene="def"
                     /locus_tag="OO160_003578"
     CDS             complement(4187..4696)
                     /gene="def"
                     /locus_tag="OO160_003578"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5525552.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4861..5985
                     /gene="dprA"
                     /locus_tag="OO160_003579"
     CDS             4861..5985
                     /gene="dprA"
                     /locus_tag="OO160_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5525553.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5957..6430
                     /locus_tag="OO160_003580"
     CDS             5957..6430
                     /locus_tag="OO160_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5525554.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6459..7001
                     /locus_tag="OO160_003581"
     CDS             6459..7001
                     /locus_tag="OO160_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5525555.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            7006..7578
                     /gene="tsaC"
                     /locus_tag="OO160_003582"
     CDS             7006..7578
                     /gene="tsaC"
                     /locus_tag="OO160_003582"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5525556.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7583..8401
                     /gene="aroE"
                     /locus_tag="OO160_003583"
     CDS             7583..8401
                     /gene="aroE"
                     /locus_tag="OO160_003583"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5525557.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8398..8655
                     /locus_tag="OO160_003584"
     CDS             8398..8655
                     /locus_tag="OO160_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5525558.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8631..9185)
                     /locus_tag="OO160_003585"
     CDS             complement(8631..9185)
                     /locus_tag="OO160_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5525559.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2359 a         2272 c         2358 g         2435 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa gacgtcgctg atggggcgct tacgtctaac aaatgttaag ccattttcct
      421 gccaggaatg aatttaatta atccttgata atcaaaggga aataataagg cgtcattaga
      481 cgccttatta attacaagaa gaaagggctt ggctggaaga gtctttcgac gtcggtaata
      541 aactttttat ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg cagattgtca
      601 ttggcaatca tcacgtcgtt tccacgtacc accgctccaa taatcgttcc tggcggtagc
      661 ttgatttcgt caatgactct gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg
      721 gcttcaatag cttctgctac gccgcggcgc aatgaggaaa caccaacaat atctgctttt
      781 cgcacatggc taagcaacgc agaaatagtt gcttgttgtg gtgaaattgc aatatcgatc
      841 acgctcccct gaaccagatc cacataagcg cgacgctgga tcaataccat cacctttttc
      901 gcacccatac gtttggcaag catggcggac atgatattgg cctcgtcatc gttggtgacg
      961 gcaataaaca gatcaacttg atcgatatgt tcttcggcca gcagttcttg atccgacgca
     1021 tcaccaaaaa agacgatcgt attctgcaac ttttccgcca gttcggcagc gcgctgctga
     1081 ttacgttcga tgagtttaac gctgtaatct ttttccagac gacgcgccag ccctgcaccg
     1141 atattaccgc caccaaccag catgatccgt ttatacggtt tttccagtcg ctgtaattca
     1201 ctcatcaccg cgcggatatg ctgtgaagcg gcaataaaga acacttcatc accagcttca
     1261 acaatggtcg aaccttgtgg acgaatgggg cgatcgtggc ggaaaattgc tgccacacga
     1321 gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg cattaccaat cagcgggccg
     1381 ccataatagg ctttaactac agcgaggctg actttaccct cagcgaagtt caccacctgc
     1441 aatgcgcctg gatactcaat cagtcggtaa atattatcga taaccaactg ctctggtgcg
     1501 atcagatgat caatcggtac agcatctgaa tgaaatagct tatcggcatc gcgtacgtag
     1561 tctggtgagc ggatgcgagc gatgcgatta ggggtgttga aaagtgagta ggctacctgg
     1621 caggcaacca tattggtttc atctgaactg gttacagcaa ccagcatatc ggcgtcgtcg
     1681 gcacctgcct cccgcaatac gcgtggatga gagccatgcc cctgcacgac tcgcaggtca
     1741 aatttatcct gcaaggtccg cagacgctca ccgttggtat cgacaacagt aatatcgttg
     1801 ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct ggccggcacc cagaatgata
     1861 attttcatca gtcgcgaccc gttatctcat cactttttga ttagcttagc gtaaaagaag
     1921 ccgtcgccct cttcggcacc aggctgattt tgtttacccg gttgctctgg tgttcctgtt
     1981 tcgcaaagtt cggcatcagc ggtacgttgc aaaaaggctt taatctgcag gctattctct
     2041 tccggtaaca ccgaacaggt ggcatagacc agggttccac cggattttaa atgcggccaa
     2101 atggcgtcga gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg atcgcgacgt
     2161 aaccatttaa tatctggatg gcgacgaatc acccccgttg ctgaacaagg cgcatctaat
     2221 aaaatgcgat caaactgttg ctcgccacac cattgggaag ggtaacggcc atcaccttgt
     2281 ttcacggttg ccttcatacc aaggcgtttt aaattgtcgt aaatgcgaga gaggcgctgt
     2341 tcgtcaatat caaccgcaac aacctgcgct tctggtgcca cctcaaggat atgcgttgtt
     2401 ttaccgccgg gggcggcaca aagatccaag atgtgttcac cgttttgtgg cgcaagccag
     2461 gtcatgcaac cttgtgctga tgcatcctga acggtaaccc atccgtcttc aaaaccaggt
     2521 agcgcatgaa caggtgcagg tgtttccagc cgtacagcat cagggtaatc cgcatgcggg
     2581 aaacctttca ttcctgcttc atccagcaat gcaagccagc tgtcgcggga atgatgcgta
     2641 cgattaatac gcagccacat tggcggacgc tggttattgg cttcggcgat ggattgccac
     2701 tgctctggat acgctttttg cagacgcttc agcaaccagg aaggatgcag ataacgtgca
     2761 tcacgggtat taaactcggc taataactct tcttgccgac gctggaactg gcgtaataca
     2821 ccgttaatca accctttaag ttgcggacgc ttaattgcga tagcgccttc aaccgtttca
     2881 gccagcgcag catgaggtgg aatgcgggta taaagcagtt gatacaaacc aaccataatc
     2941 aggtaatgca cagtgcgctg tttgccggtc atcggacggg ccattaactt attaatcagc
     3001 cagtctaact gcgaaagcgt acgcagtacg ccaaagcaca actcttgaag aagtgctttg
     3061 tctttatcgg aaactttttg ctggagcggt ggcagaatgt tgcttaatga ttgcccttgc
     3121 tcgacgactt gttcaacggc ctgggccgcc atgctacgta aattacgttg ttttttcata
     3181 agtataaaaa tgctcggcaa gaccgggctt agaagagtgg actatcagac cagacggttg
     3241 cccggaacaa accattcccg acgagagttc aggaggtctt gcgcgctcat cgctttctta
     3301 cccgcaggtt gtaacgagag caggttcagg atgccatcac cagtcgcaac ctgaatgcct
     3361 tgtttgttgg cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc aatgaccgac
     3421 gctttccaga ctttaaccgg ctgtccttca atttccagcc agctcattgg ccatgggttg
     3481 aaagcgcgaa tgcagcgttc aagctgtgct gccgaaagtg accagtcaat acgcgcttct
     3541 tctttactca acttctcggc gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc
     3601 gtgccgtctg ccaattgttt caacgtggtg ataagccctt gtgggccaag ctctgccagc
     3661 ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg ggcaggagag cttatagagc
     3721 atgtcaccgg tgtctaaacc aacatccatt tgcataatgg ttacaccagt ttctgcatca
     3781 cccgcccata gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag cagtgaacca
     3841 tgaatgttga tacagccaag acgcggcatc tccagcacag ctttaggcag aattaaacca
     3901 taggcgacga cgaccataac atcagcctgc aaatcggcga ccagttgctg gttttcttgt
     3961 ggacgcaggg aaacaggttg aaaaacgggc agaccttctt cctcagccag aactttaatc
     4021 gggctgggca tcagtttttt accgcgtcct gccggtcggt ctggctgggt gaacacgcca
     4081 acgacgttat gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta
     4141 cccgcaaaaa taatacgtag tgattctgac acgttagttc ttatccttaa gcccgggctt
     4201 tcagacgatc cagtttttca actttctgac gaattcgttg ttgtttcagc ggtgacagat
     4261 aatccataaa cagtttgcca accaggtgat ccatctcatg ctgaatacag atagctaaca
     4321 gaccctctgc ttccagttca aatggtttac cgtcgcggtc aagggcgcga attttaactt
     4381 tctctgcgcg cggcactaaa gcacgttgtt cagggatcga caggcaacct tcttcaatgc
     4441 ctgtttcgcc gcttttttct aaaagctccg gattgattaa caccagccgt tcgtcacggt
     4501 tttccgaaac atcaataaca atgatacgtt gatggatatc aacctgggtt gccgccaggc
     4561 caataccttc ttctgcgtac atcgtctcga acatatcatc gacgatacgc tgaatttctg
     4621 cattcacttc ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca
     4681 cttgcaaaac tgacataaat ctccagagat gtgttcagga gttagaaaga ttatttcttc
     4741 tattctagac aaatccccct ctgattgaca gcatcacttg attgacagca tcacttgatt
     4801 gacagcatca ctgaccaatc gcaaagattg ctaaggcttg cttatggcag ggagataagg
     4861 atggtcgata tagaaatttg gctgcgttta atgagcatca gcagcttgta cggcgatgat
     4921 atggtccgta tcgctcactg gctggcaaaa cagtcgcata ttgatgcggt tgtattgcag
     4981 caaacagggc ttacattgcg gcaggcacaa cgctttcttt catttccacg aaagagtatc
     5041 gaaagctcac tttgttggtt ggagcaaccc aaccatcatt taatccctgc ggacagcgaa
     5101 ttttatcctc ctcaacttca ggcgacgaca gattaccccg gcgcactgtt tgttgaagga
     5161 gaactgcacg cgctgcattc atttcagctt gccgtagtgg ggagtcgggc gcattcatgg
     5221 tatggcgagc gatgggggcg attattttgc gaaactctgg cgacgtgtgg agtgacaatt
     5281 acgagtggac tggcgcgtgg aatcgatggt gtggtgcata aagcggcctt acaggtaaat
     5341 ggcgtcagca ttgctgtatt ggggaatgga cttaatacca ttcatccgcg tcgccatgcc
     5401 cgactggctg ccagtctgtt tgaacagggt ggcgctctcg tctcggaatt tcccctcgat
     5461 gttccacccc ttgcttacaa tttcccacga agaaatcgca ttatcagtgg tctaagtaaa
     5521 ggtgtactgg tggtggaagc ggctttgcgc agtggttcgc tggtgacagc acgttgtgcg
     5581 cttgagcagg ggcgtgaagt ttttgccttg ccaggtccaa tagggaatcc gggaagcgaa
     5641 gggcctcact ggttaataaa acaaggtgcg attcttgtga cggaaccgga agaaattctg
     5701 gaaaacttgc aatttggatt gcactggttg ccagacgccc ctgaaaattc attttattca
     5761 ccagatcagc aagacgtggc attgccattt cctgagctcc tggctaacgt aggagatgag
     5821 gtaacacctg ttgacgtcgt cgctgaacgt gccggccaac ctgtgccaga ggtagttact
     5881 caactactcg aactggagtt agcaggatgg atcgcagctg tacccggcgg ctatgtccga
     5941 ttgaggaggg catgccatgt tcgacgtact aatgtatttg tttgaaacct atattcacac
     6001 tgaagctgag ttgcgtgtgg atcaagacaa acttgaacag gatcttaccg acgcaggttt
     6061 tgagcgagaa gatatctaca atgccctgct atggctggaa aaacttgctg attatcagga
     6121 agggttggca gaaccgatgc aactggcctc ggatcctctc tccatgcgta tttatacacc
     6181 ggaagagtgt gaacgactgg atgccagctg ccgtgggttt ctgcttttcc ttgagcagat
     6241 tcaggtgctc aaccttgaaa ctcgtgaaat ggtgatagag cgagtgctcg cgctggataa
     6301 cgcagagttc gaactggatg atctgaaatg ggtgatcctg atggtgttgt tcaacatccc
     6361 gggctgcgaa aatgcgtacc agcaaatgga agaattactc tttgaagtga atgaaggtat
     6421 gctgcattaa tcatttcttt aattcagcat aagttgttat ggcaaaatca gcactgttca
     6481 cggtgcgtaa taatgagtcc tgcccaaagt gcggggctga actggttatt cgatccggga
     6541 aacacggtcc gtttcttgga tgctcacagt atccggcgtg tgactacgtc cgtcctctga
     6601 aatcttcagc ggatggacat attgtcaaag ttctggaggg gcaggtttgc cccgtatgtg
     6661 gcgcaaatct ggtattacgc cagggacgct ttggtatgtt tattggttgc agtaactacc
     6721 ctgaatgcga acataccgaa cttatcgata aaccggacga aacagcaatt acatgccccc
     6781 aatgtcggac gggccatctg gtccagcgcc gctcccgtta tggcaaaaca tttcactcct
     6841 gtgatcgcta cccggagtgt caatttgcca ttaacttcaa accaatagct ggagaatgcc
     6901 ctgagtgtca ttatccgcta ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt
     6961 gtgccagtaa acaatgtgga aagccggttt cggcggaata ataacgtgaa taataacctg
     7021 caaggagacg ctatcgcagc tgcgatagat gtcctcaatg aaaaacgtgt catcgcctat
     7081 ccaacggaag ccgttttcgg tgtcgggtgc gatcctgata gcgaaacagc agtgatgaga
     7141 ctgttggagt taaaacagcg tccggttgat aaggggctga ttttaatcgc ggcaaattac
     7201 gagcagctta aaccctatat tgatgacacc atgttgactg acgtgcagcg tgaaaccatt
     7261 ttttcctgct ggccaggtcc tgtcaccttt gtctttcccg cgcctgcgac aacaccgcgc
     7321 tggttgacgg gccgctttga ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt
     7381 gctttgtgcc aggcttatgg taaaccgctg gtttctacca gtgccaactt gagtggattg
     7441 ccaccttgtc gaacagtaga cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg
     7501 cctggtgaaa cgggggggcg tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa
     7561 ctgtttcgac aggggtaaca taatggaaac ctatgctgtt tttggtaatc cgatagccca
     7621 cagcaaatcg ccattcattc atcagcaatt tgctcagcaa ctgaatattg aacatcccta
     7681 tgggcgcgtg ttggcaccca tcaatgattt catcaacaca ctgaacgctt tctttcgtgc
     7741 tggtggtaaa ggtgcgaatg tgacggtgcc ttttaaagaa gaggcttttg ccagagcgga
     7801 tgagcttact gaacgggcag cgttggctgg tgcagttaat accctcaagc ggttagaaga
     7861 tggacgcctg ctgggtgaca ataccgatgg tgtaggcttg ttaagcgatc tggaacgtct
     7921 gtcttttatc cgccctggtt tacgtattct gcttatcggc gctggtggag catctcgcgg
     7981 cgtactactg ccacttcttt ccctggactg tgcggtgaca atcactaatc ggacggtatc
     8041 ccgcgcggaa gagttggcta aattgtttgc gcacactggc agtattcagg cgttgggtat
     8101 ggacgaactg gaaggtcatg agtttgatct cattattaat gcaacatcca gtggcatcag
     8161 tggtgatatt ccggcgatcc cgtcatcgct cattcatcca ggcatttatt gctatgacat
     8221 gttctatcag aaaggaaaaa ctccttttct ggcatggtgt gagcagcgag gctcaaagcg
     8281 taatgctgat ggtttaggaa tgctggtggc acaggcggct catgcctttc ttctctggca
     8341 cggtgttctg cctgacgtag aaccagttat aaagcaattg caggaggaat tgtccgcgtg
     8401 aatcaggcca tccagtttcc ggacagggaa gagtgggacg agaataaaaa atgtgtatgt
     8461 tttcccgctc tcgtgaatgg tatgcaactg acatgcgcga tctctggcga gagtctggcg
     8521 tatcgcttta ctggagatac gccagaacag tggttagcga gttttcgtca gcatcgctgg
     8581 gacctggaag aagaagcgga aaacttaatt caggaacaaa gtgaagatga tcaaggctgg
     8641 gtctggttac cctgatccag atattcgtcc ttccatttca cgtaattatt cgcggaatag
     8701 cgtaacccag ccttctcttc atcacttaac gggcggatct gtttgacggg gctaccgaga
     8761 tacagatatc cgctctccag ccgtttattt tgtgggacca gactacccgc accaatcatc
     8821 acatcatctt ctactattgc gccatcaagt aaaattgagc ccatcccaac caaaactcga
     8881 ttgccaatgg tgcagccgtg gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg
     8941 gttaatgggt tgccatctgg gttgtacgag gatttatgag tgacatgcaa catactgcca
     9001 tcctggatat tggtgcgtgc tccgatctgt acataatgta catctccacg aatcacaacg
     9061 agcggccaga tccccacatc atcagccaga cgaacgtcac caatcacgac actgctatcg
     9121 tcgatcatta cgcgctgacc gatttgtgga aaaagatcgc ggtatgggcg taaaacatca
     9181 gacatactta cctcagcaat aaatgattta ctaatgactt tgggggcatt attggccttg
     9241 tgcaagtctt ttagtatgca aaaaagcacc gttttgtgtg cgattgcagc aaaaagggtg
     9301 aaaaaacaac aaacagaaaa gaagatcaaa aaaatacttg tgcaaaatat tgggatccct
     9361 ataatgcgcc tccgttgaga cgacaacgtg aaacacttca caggatggtc ggaacaacga
     9421 agag
//
LOCUS       DAJTNP010000132         9417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTNP010000132 DAJTNP010000000
VERSION     DAJTNP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..496)
                     /gene="pflA"
                     /locus_tag="OO160_003586"
     CDS             complement(<1..496)
                     /gene="pflA"
                     /locus_tag="OO160_003586"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR5525560.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVW"
     gene            complement(688..2970)
                     /gene="pflB"
                     /locus_tag="OO160_003587"
     CDS             complement(688..2970)
                     /gene="pflB"
                     /locus_tag="OO160_003587"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5525561.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3025..3882)
                     /gene="focA"
                     /locus_tag="OO160_003588"
     CDS             complement(3025..3882)
                     /gene="focA"
                     /locus_tag="OO160_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5525562.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4288..6048)
                     /locus_tag="OO160_003589"
     CDS             complement(4288..6048)
                     /locus_tag="OO160_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5525563.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6178..6804
                     /locus_tag="OO160_003590"
                     /pseudo
     CDS             6178..6804
                     /locus_tag="OO160_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7003..8091
                     /gene="serC"
                     /locus_tag="OO160_003591"
     CDS             7003..8091
                     /gene="serC"
                     /locus_tag="OO160_003591"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5525564.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8162..>9417
                     /gene="aroA"
                     /locus_tag="OO160_003592"
     CDS             8162..>9417
                     /gene="aroA"
                     /locus_tag="OO160_003592"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5525565.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFE"
BASE COUNT         2385 a         2257 c         2368 g         2407 t
ORIGIN      
        1 tccacacctt cacatttttg ttcgccagat atttagcgaa ctccagcgtg cggtggttgg
       61 aaactccaac cagattttgg tggatctcgt cgttcatctg tttgagatcg agcattacca
      121 ggtcggttac ttccagcagt tcatcaatca ccggatcgta acgacgaaca aaaccgttgg
      181 tatccagaca ggtatgaatg ccttcttttt tacaggcgcg gaaccagtca cgaacaaact
      241 cagcttgcag gattgcttca ccgccggatg cggtaacgcc gccgccggaa gcgttcataa
      301 agtggcgata ggtcaccact tccttcatca aatcttcaac ggtaacttct ttaccgccat
      361 gcgtgtccca ggtgtcgcgg ttatgacaat acaggcagcg catcaggcag ccctggaaaa
      421 aggtgataaa gcgaatacct gggccgtcta cggttccaca ggattcaaag gagtgaatgc
      481 gaccaataac tgacattgcg gtgtttctcc agatgtggcc catctgaggc cgtgttggtg
      541 cgcagctcga aggctacgtc gagtctgttt tggcagtcac cttaaagtat agatagctga
      601 caaaaaaggc tctcgtgcta aaaaaggccc cactttcgtg gagcctttat tgtacgcttt
      661 ttactgtacg atttcagtca aatctaatta catagattga gtgaaggtac gagtaataac
      721 gtcctgctgc tgttctttag tcagcgagtt gaaacgtact gcgtagccag atacacggat
      781 ggtcagctgc ggatattttt ccgggttttc catcgcgtcg agcagcattt cacggttcat
      841 cacgttaacg ttcaggtgct gaccaccttc gatggatgct tcgtggtgga agtaaccatc
      901 catcagacca gccaggttgg tcttacgaac ttcgtcgtct ttacccagtg cgttcggaac
      961 gatagagaag gtgtaggaga taccatcttt agcgtaagca aacggcagtt tagcaacgga
     1021 agtcagagac gctacagcac ctttctggtc acgaccgtgc atcgggttag cacccggtcc
     1081 gaacggcgcg ccagcacgac gaccgtctgg ggtgttacca gttttcttac catacacaac
     1141 gttagaagtg atggtcagaa cagactgagt cgggatagcg tcacggtagg tgtgcagttt
     1201 ctgaattttc ttcatgaaac gttctaccag gtcaacagcc aggtcatcta cacgcggatc
     1261 attgttacca aactgcgggt attcgccttc gatttcgaag tcgatagcca gaccgtcttc
     1321 gtcacgaatc ggtttaactt tcgcatattt gattgcagac agggagtcag cagcaacgga
     1381 cagaccagcg ataccacacg ccatggtgcg gataacgtca cggtcgtgca gcgccatcag
     1441 agaggcttcg tagctgtact tgtcgtgcat gtagtggatg atgttcagtg cagtgatgta
     1501 ctgtttagcc agccagtcca tgaagtgatc catgcgctcc atcacttcat cgtagctcag
     1561 gagatcgcct ttgatcggtt cagacttcgg accaacctgc attttcagtt tttcgtcaac
     1621 gccgccgttg attgcgtaca gcatggtttt cgccaggttt gcacgcgcac cgaagaactg
     1681 catttgttta ccaacgatca tcgggcttac gcagcaagcg atagcgtagt catcgttgtt
     1741 gaagtccgga cgcatcaggt catcgttctc atactgcaga gaagaggtgt cgatggacac
     1801 tttagcggcg aatttcttga agttcagcgg cagtttttca gaccacagaa tggtcatgtt
     1861 cggttccgga gacggcccca tagtgtacag ggtgttcagg aaacggaagc tgtttttggt
     1921 aaccagggta cgaccatcga ggcccatacc accgatagat tcggttgccc agattgggtc
     1981 gccagagaac agttcatcgt attccggagt acgcaggaag cgaaccatac gcagtttcat
     2041 gaccaggtgg tcaaccattt cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc
     2101 acgttcgatg tacacatcca ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt
     2161 ctgagactta acagcagcca ggtagccgaa gtaagtccac tggatagctt cctgagcgtt
     2221 ggtagccgga ccagagatgt cgtagccgta tttcgcagcc atttctttca tctgacccag
     2281 agcgcggtgc tgttcagcga tttcttcgcg cagacggata gtctgttcca ggtttacgcc
     2341 gttttccaga tcagcctgta gagaggtgaa ctgagcgtat ttgtctttca tcaggtagtc
     2401 gataccgtac agcgcaacgc gacggtagtc accgatgata cggccacggc cataagcatc
     2461 tggcagaccg gtcagaacac cggatttacg gcaacgcagg atgtccggag tgtaaacgtc
     2521 gaacacgccc tggttgtgag ttttacggta ttcagtgaag atttttttga tcatcgggtc
     2581 cagttcgcgg ttgtacgctt tgcaggaacc ttcgatcatt ttgataccac cgaacgggat
     2641 aagagcacgt ttcagcggag cttcagtctg cagaccaaca actttttcca acgctttatt
     2701 gatgtagcca gcgtcgtgag aggtgatggt ggaagcaaca gcggtgtcaa aatcaactgg
     2761 cgcgtgagtg cggttttcca gtttaacacc ttccattact ttgtcccaca gggtggtggt
     2821 cgcttcagta gcgccagcca ggaaggactc gtcaccctcg tacggagtgt agtttttctg
     2881 aatgaagtca cggacgttta cttcattctg ccagtcacct ttggtaaaac cttcccaggc
     2941 tgtggctaac ttttcattaa gctcggacat gtaacaccta ccttcttaag tggatttttt
     3001 atttactgcg tacttcgaca accattaatg gtgatcgttt tcacgcaggt aaatgaccca
     3061 gtatgtcaac ccaaccaaca aaccaccgcc gataatatta ccgatcgtaa ccgggatcag
     3121 gttatcagtg atgaagttca tcacggtcag gtgagaaaaa ttttccggtg cagaaccgac
     3181 agcggtccag aattccggag atgcgaagtc gcggattaca atacccatcg ggatcataaa
     3241 catgtttgcg atactgtgct caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat
     3301 aatgaacgct ttgtccatca ggctgcggcc agaataactc atccatactg ccagacatac
     3361 catcaggttt gccaggatac caagacagac ggcctcaata aaagtatggt gcactttgtg
     3421 gtcggcggtt tgtaggacgt ttagtcccca ttgaccattt gcggtcatat actcgccgga
     3481 aagccacatt aaaagtacaa acagcagtgc gccgaccagg ttgccaaaat agacatttag
     3541 ccagttttta gccaactgac accaggtgat gcgcccactc gccttagcaa caacaatcaa
     3601 cactgtggaa gtaaagagat cggctccgca gacaacacaa agaatcagcc ccagagagaa
     3661 gcaaatgccg ccaaccagtt ttgccatgcc gaagggcatt gtgcctgtgc cagtggttgc
     3721 tgtgatatag aagacgaatg cgattgagat gaaaacaccg gcggtaatcg ccagatagaa
     3781 agtcttaagc ggatgtttcg ttgctttata gacacccgcc tcttcggcca ctttggccat
     3841 tgcagcagga agtaaaagat caaaagggtt gtcagctttc acactaactc tctctttatt
     3901 aagtcggcga cgagatacta acaaagcatt atagatgaga aattgatata gatcatatct
     3961 cgcctggctt ataggcccgt aactcgcatg gtttttatgc aaatacggag taactatttg
     4021 attatccaaa taaaaataaa ttttaaaaat taacaaatga gttgaatttt ttccgcatcc
     4081 tccgctaaaa cagttaatta aaagggagca tcaggcgaat aaagtaacaa tatcgatcgt
     4141 atttattaaa tacaaattac cgatatttaa ccttataatt acaattattt tattaatgca
     4201 aatatatgta aagcggggca ttaaaaaaac gccccgtaat ataactcaga ctaatcatta
     4261 agcctacatt gcgtaggcta tttgatttta ttttgcccag aatgctgctt tggcgcgctg
     4321 cagcttttcg taggccttca acaacgactg atgtgcagca aacgcgtgca gatcgctatc
     4381 taccggttgc aggccgtaaa acgccgcttc gccgctcatt gccgcactgg cggcttctac
     4441 ggcatctgcg ccgtacatgc gaacaaacgc attcagatat tgcagcggct ggcgatcttc
     4501 ttcctgtgcc agtaataaca acgtttgcag gcagcgataa tagttggcgc gttccggact
     4561 aaatactgat gagttaaact ccatcgtcca ttcggtccag accagagcct gttccagatc
     4621 gccaccagcc agcgccagca tggcttttaa ttcaccgata cgcagggtgt accaaccgtt
     4681 atccgacccg gtcgccagac ccaacagctc acgcacgcgg gtaaagtcat caaaaccttc
     4741 ttcatccagt tgctcgatga ggttcaggta atcttctttt tcccactcgc tgcctggtag
     4801 cgaaagaatc gtttcacgta aatggctgcc catactgtta ttcgcgagcc acagatcttc
     4861 agccggataa atatcggaca tgccaggcac gataatacgg caagcataaa cgcccagatg
     4921 ctcgtaatcg gcaatataaa cttctttatc ttctttgttg aagatagcca tcagcgtagc
     4981 aaactcttct tccgtggtgc cggagaaatt ccagtccaca aacggataat cggcatcctg
     5041 cttgaacagg tcccaggaga ttaaaccgct ggaatcgata aagtgcgttt cgaggttggt
     5101 atgttcagcg acttcttcat catcgaaggt tggcggagta aacacatcca aatctttcag
     5161 gccacgacct tgcagcagct cggtcacggt acgttccagt gctacgccaa aatcaggatg
     5221 cgcaccgaaa gaggcaaagc aggtgccgtt tgcaggatta aacagtacca cgcaaatcac
     5281 cggatactgg ccgccaaggg aaccatcata tgcgaagatc gggaaacctt ctgtttccag
     5341 tgtttcgatc gcttcaacca ccgctgggta acgcgccagc acgtctgccg ggatttccgg
     5401 caagctgatg ctttcagcaa taatgcggtt tttcacgtag cgttcgaaaa cttcggacaa
     5461 cccctgaacg cgtgcttcgt tgcgggtatt gccagcggac ataccgttag aaacgtacag
     5521 gttaccaatg atattcatcg gaatataaac ggtctgattg tcggactggc gcgtaaacgg
     5581 cagaccgcaa ataccacgat cttcgttacc ggattgtagg tcaatcagca tactgccggt
     5641 caattcattc tccggatcgt aaaatgcgcg cagacggtca tcgagcagcc cttctggcac
     5701 atcgtcattt tcgctcagtg ggaaccattt ttcgttggga taatgcacaa atggaccgtt
     5761 ggcgatggtt tcgcccagcc agaaatcagc aaaaaagtag ttggttgaga gacgctcgaa
     5821 atattcaccg agtgcagaag ccagcgccgc tttcttggtt gcgcctttac cgttggtaaa
     5881 acacagtgcg cactctttgt cgcgaatatg tacagaccag acgttaggca cgggattcag
     5941 ccaggaggcc tcttcaatct gaaagccgag gtctgaaagt ttttgctgga agcgagcgat
     6001 ggaatcttcc agagcggcat ctttgccggg gataaatgtt tgcgtcatga aaatcacttt
     6061 agtcgtacgg aaagcgcgca ataatacggg ttttatctca aaggcgctat caccgccgcc
     6121 atgccgatga actgttgact atgcttttag cggataaacc acgttaagag cataaaaatg
     6181 aaggcgttcg atctccaccg tatggcattt gataaagtgc cttttgattt ccttggcgaa
     6241 gttgcactac gtagtcttta tacctttgta ctggtttttt gttcctcaaa atgaccggaa
     6301 gacgcggtgt gcgacagatg tcgctgtttg aagtgttaat cattctcacg ctgggttcag
     6361 cggcgggaga tgtggcgttt tatgatgatg tgccgatggt gccggtactt atcgtcttta
     6421 tcactctggc gttgttatac cgcctggtaa tgtgatggcg agctggcctg gtcgaaactc
     6481 aataactcca acatgacgga atttgagttc tttatggagc tacgattgcg tggcgtggag
     6541 cagctggggc aggtacgtct ggcgattctc gaaaccaacg ggcaaatcag tgtctatttc
     6601 tttgaagatg acaaggtgaa accgggttta cttattttac ccagtgattg tacgcagcgt
     6661 tacaaagtgg tgccggagtc ggcggactat gcctgcattc gttgtagtga aatcattcat
     6721 atgaacgcgg gggaaaaaca attatgtccg cgctgtgcaa atccagaatg gacgaaggca
     6781 agtcgggcaa aacgggtgac ctgacagtaa aaacatcggc tttttgctaa taatccgaga
     6841 gattcttttg tgtgatgcaa gccacatttt tgccctcaac ggttttactc attgcgatgt
     6901 gtgtcactga atgataaaac cgatagccac aggaataatg tattacctgt ggtcgcaatc
     6961 gattgaccgc gggttaatag caacgcaacg tggtgagggg aaatggctca aatcttcaat
     7021 tttagttctg gtccggcaat gctaccggca gaggtgctta aacaggctca acaggaactg
     7081 cgcgactgga acggtcttgg tacgtcggtg atggaagtga gtcaccgtgg caaagagttc
     7141 attcaggttg cagaggaagc cgagaaggat tttcgcgatc ttcttaatgt cccctccaac
     7201 tacaaggtat tattctgcca tggcggtggt cgcggtcagt ttgctgcggt accgctgaat
     7261 attctcggtg ataaaaccac cgcagattat gttgatgccg gttactgggc ggcaagtgcc
     7321 attaaagaag cgaaaaaata ctgcacgcct aatgtctttg acgccaaagt gactgttgat
     7381 ggtctgcgcg cggttaagcc aatgcgtgaa tggcaactct ctgataatgc ggcttatatg
     7441 cattactgcc cgaatgaaac catcgacggt atcgccatcg acgagacgcc agacttcggc
     7501 aaagatgtgg tggtcgccgc cgacttctct tcaaccattc tttcccgtcc gattgacgtc
     7561 agccgttatg gtgtaattta cgctggcgcg cagaaaaata tcggcccggc tggcctgaca
     7621 atcgtcatcg ttcgtgaaga tttgctgggc aaagcgaata tcgcgtgtcc gtcgattctg
     7681 gattattcca tcctcaacga taacggctcc atgtttaaca cgccgccgac atttgcctgg
     7741 tatctatctg gtctggtctt taaatggctg aaagcgaacg gcggtgtagc tgaaatggat
     7801 aaaatcaatc agcaaaaagc agaactgcta tatggggtga ttgataacag cgatttctac
     7861 cgcaatgacg tggcgaaagc taaccgttcg cggatgaacg tgccgttcca gttggcggac
     7921 agtgcgcttg acaaattgtt ccttgaagag tcttttgctg caggccttca tgcactgaaa
     7981 ggacaccgtg tggtcggcgg aatgcgcgcg tctatttata atgccatgcc gctggaaggc
     8041 gttaaagcgc tgacagactt catggttgag ttcgaacgcc gtcacggtta atgccgaaat
     8101 tttgcttaat ccccacagcc agcctgtggg gtttttattt ctgttgtaga gagttgagtt
     8161 catggaatcc ctgacgttac aacccatcgc tcgtgtcgat ggcactatta atctgcccgg
     8221 ttccaagagc gtttctaacc gcgctttatt gcttgcggca ttagcacacg gcaaaacagt
     8281 attaaccaat ctgctggata gcgacgacgt gcgccatatg ctgaatgcat taacagcgtt
     8341 agggttaagc tatacgcttt cagccgatcg tacgcgttgc gaaattatcg gtaacggcgg
     8401 tccattacac gcagaaggtg ccctggagtt gttcctcggt aacgccggaa cggcaatgcg
     8461 tccgctggcg gcagctcttt gtctggatag caatgatatt gtgctgaccg gtgagccgcg
     8521 tatgaaagag cgcccgattg gtcatctggt ggatgcgctg cgcctgggcg gggcgaagat
     8581 cacttacctg gaacaagaaa attatccgcc gttgcgttta cagggcggct ttactggcgg
     8641 caacgttgac gttgatggct ccgtttccag ccaattcctc accgcactgt taatgactgc
     8701 gcctcttgcg ccggaagata cggtgattcg tattaaaggc gatctggttt ctaaacctta
     8761 tatcgacatc acactcaatc tgatgaagac gtttggtgtt gaaattgaaa atcagcacta
     8821 tcaacaattt gtcgtaaaag gcgggcagtc ttatcagtct ccgggtactt atttggtcga
     8881 aggcgatgca tcttcggctt cttacttcct ggcagcagca gcaatcaaag gcggcactgt
     8941 aaaagtgacc ggtattggac gtaacagtat gcagggtgat attcgctttg ctgatgtgct
     9001 ggaaaaaatg ggcgcgacca tttgctgggg cgatgattat atttcctgca cgcgtggtga
     9061 actgaacgct attgatatgg atatgaacca tattcctgat gcggcgatga ccattgccac
     9121 ggcggcgtta tttgcaaaag gcaccaccac gctgcgcaat atctataact ggcgtgttaa
     9181 agagaccgat cgcctgtttg cgatggcaac agaactgcgt aaagtcggcg cggaagtgga
     9241 agaggggcac gattacattc gtatcactcc tccggaaaaa ctgaactttg ccgagatcgc
     9301 gacatacaat gatcaccgga tggcgatgtg tttctcgctg gtggcgttgt cagatacacc
     9361 agtgacgatt cttgatccca aatgcacggc caaaacattt ccggattatt tcgagca
//
LOCUS       DAJTNP010000133         9239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTNP010000133 DAJTNP010000000
VERSION     DAJTNP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO160_003593"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO160_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5525566.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO160_003594"
     CDS             1828..3147
                     /locus_tag="OO160_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5525567.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO160_003595"
     CDS             3445..3486
                     /locus_tag="OO160_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525568.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO160_003596"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO160_003596"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5525569.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO160_003597"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO160_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO160_003598"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO160_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO160_003599"
     CDS             5815..6711
                     /locus_tag="OO160_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5525570.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO160_003600"
     CDS             6708..7682
                     /locus_tag="OO160_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5525571.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO160_003601"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO160_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO160_003602"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO160_003602"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5525572.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2257 a         2257 c         2316 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctc
//
LOCUS       DAJTNP010000134         9221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTNP010000134 DAJTNP010000000
VERSION     DAJTNP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..643
                     /gene="hchA"
                     /locus_tag="OO160_003603"
     CDS             <1..643
                     /gene="hchA"
                     /locus_tag="OO160_003603"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5525573.1"
                     /translation="TGNHPIETLLPLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDE
                     KVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQ
                     WAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTPDIGYMPGHLTWY
                     FGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(751..2109)
                     /gene="hprS"
                     /locus_tag="OO160_003604"
     CDS             complement(751..2109)
                     /gene="hprS"
                     /locus_tag="OO160_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5525574.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2109..2780)
                     /gene="hprR"
                     /locus_tag="OO160_003605"
     CDS             complement(2109..2780)
                     /gene="hprR"
                     /locus_tag="OO160_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5525575.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2913..3326
                     /gene="hiuH"
                     /locus_tag="OO160_003606"
     CDS             2913..3326
                     /gene="hiuH"
                     /locus_tag="OO160_003606"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5525576.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3435..4439
                     /gene="msrP"
                     /locus_tag="OO160_003607"
                     /pseudo
     CDS             3435..4439
                     /gene="msrP"
                     /locus_tag="OO160_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4440..5075
                     /gene="msrQ"
                     /locus_tag="OO160_003608"
     CDS             4440..5075
                     /gene="msrQ"
                     /locus_tag="OO160_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5525577.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5333..5983
                     /gene="zinT"
                     /locus_tag="OO160_003609"
     CDS             5333..5983
                     /gene="zinT"
                     /locus_tag="OO160_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5525578.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7061..7330)
                     /locus_tag="OO160_003610"
     CDS             complement(7061..7330)
                     /locus_tag="OO160_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5525579.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7387..8055
                     /locus_tag="OO160_003611"
     CDS             7387..8055
                     /locus_tag="OO160_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5525580.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8371..>9221
                     /locus_tag="OO160_003612"
     CDS             8371..>9221
                     /locus_tag="OO160_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5525581.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEE"
BASE COUNT         2646 a         2083 c         1884 g         2608 t
ORIGIN      
        1 gaccggtaac catccgattg aaacgttgct gccgttgtat catctccatg ctgcaggttt
       61 cgaattcgaa gtggcgacca tttccggtct gatgaccaag tttgaatact gggctatgcc
      121 gcacaaagat gaaaaagtaa tgccattctt tgaacagcat aaatcgttat tccgtaatcc
      181 gaagaaactc gcggatgttg tcgccagcct caacgctgat agcgaatatg cagcaatctt
      241 tgttcctggt gggcatggcg cacttattgg tttaccagaa agccaggacg tggctgccgc
      301 tttacagtgg gcaatcaaaa atgaccgttt tgttatctcc ctttgccacg gtccggcggc
      361 ttttctggca cttcgccaca gcgataaccc actgaatggt tattctattt gcgcattccc
      421 agacgccgca gacaaacaaa cgccagatat tggctatatg ccgggtcatc tcacctggta
      481 cttcggcgaa gagttgaaga aaatgggcat gaacatcatt aatgacgaca tcaccgggcg
      541 agtacataag gaccgtaaac gtctcaccgg cgacagtcct tttgcagcga atgcgttggg
      601 taaactggcg gcgcaggaaa tgctggcagc ttacgcgagt taatccctgt tacattgtga
      661 tgtaagcgca taatcaatcg cagccacgtt aatactcacg acgtggctgc gttttttcac
      721 ggttaactta tggcgtactg aagcccgatg ttaatttctt tgcggtaacg taattcggaa
      781 cacgttatgc ttactgagat agtgatacgt agcacttcct ccatgtaatt cggcaatcgc
      841 tttgactaaa gaaaggccaa gtccctgacc tacggaatga cgcgaattat ctccccgcca
      901 aaatctacgg aagagttttt caggctcatt aattttcgtt ccaggactgg cgatatcaat
      961 attaagagag ccgttggcat caagaaaact ggttatatga atacgcgatt tttctggtga
     1021 atatcgaatg gcgttaacaa taagattcga taacattcgt tgcagtaaaa ttttatccgc
     1081 aaagatttgt tgattgcact tgaccttaaa gcaaatctct ttctcgtctg aaagatattc
     1141 aagatagtcc aaaagatttt caacttcctt attgagagaa agcgaatcca gtttcaccaa
     1201 aacattgttt ttgtctgccc tggcaagaaa cagtatattc tctgttaacc gcgaaatatt
     1261 ttccagctct tcaatgtttc cggcaattgt tttttgatat tcagcgatac ttctgatttg
     1321 actgagcgta acctgattct gacccagtaa tgcattaatt ggcgttctaa gttcatgagc
     1381 gagatcgtca gcaaattgac taaggcgctc aaaatctttg actaaagcat gatgcatttt
     1441 attcaacgcc tgcccaagag gttttagttc tctcggtaat gcgttaacct caaccggcac
     1501 tcggctatcg ttataattca gcgcttccgt tacgccactc aactttttga tctctcgcaa
     1561 ccccgtcctg attaacaacg gacttaatac tgagcacagt ataatggcga caatgcaaat
     1621 tataatgcta ttaattttat actgttcaag catattatgt ctggctgaag ccaatttagc
     1681 cacagtaacc gttaatgatg gcgaaacttc atcaatgtta attcgtaaag catctatctc
     1741 tgtatcatta ataatgcttc tgtaaatgcc tgctgcgctg attgtctcac tagcaggtat
     1801 gttatttaac atgtcatcac tgacatttgt ccggttaaca attttattga tgccatcact
     1861 atgaatgatc aaaatatcct gactaacatc catcatccgg ttaaagtaca caggtaacgt
     1921 atctggattt accccatcaa ttaacaactg cttgatctgc gctgtccggt taatgagtgt
     1981 tgtatcatcg cgccatttca actcacttgc caggccatta tagagagtcc atacaattcc
     2041 agcgccagca acagactgca gaaatataaa aagcaaggtt aaacggacgg ttatagataa
     2101 tcttttcatt atttttttac cgctacgaat gaatagccca tcccccggat tgtggcgatt
     2161 agcttttcag gaaaaggatc atcaactttt gcgcggagcc tgcgaatggc gacatccacc
     2221 gtattggtat cactatcaaa gttgattccc caaatttcac tcgcaataac cgttctgggt
     2281 ataatctcgc cagctctgga ggccagtagc caaagtaact gaaactcctt gcgcgtcagt
     2341 gtaatactga tattgtccct gctgacactt tgactgacag agtccatttt taagccactg
     2401 atttctaatg ttgaattcag agcgtgatgt tgccttaatt gtgcccgaac ccttgctagc
     2461 aactcagaaa atgaaaaagg ttttaccaga taatcatttg ccccactgtc caaccctctg
     2521 actctgtcat cgacagaatc ccttgcagta aggcaaataa caggggtttg ctttgccgtt
     2581 cttaacgttt gtaagatctg ccagccatcc atacccggaa gcataatatc cagaatgatc
     2641 aatgcataat catccttcag cgcaagataa agcccatctc tgccatcaga aacggcatca
     2701 atgacataac ccgcttcgga aagcccctgc gttacccatt cctgggtcct ttgattatct
     2761 tcaataagta gaatcttcat gaaaataata tgccataaat catatgttat agaaacaacc
     2821 tggttcatta caaaattgta atgctgctgt aaggttaccc tggacgtttt ttcgctatcc
     2881 tcaaaactca ttcacatgac aaggatataa acatgttaaa gcgttattta gtactctcgg
     2941 tagcaacggc agccttttca ttaccttctt tggtttatgc cgcacaacaa aacattctta
     3001 gcgtgcacat tttgaaccag caaacaggaa aacccgctgc cgacgtgaca gtcactcttg
     3061 aaaagaaagc ggataacggc tggttacaac ttaataccgc caaaacagat aaggatggac
     3121 gaattaaggc actgtggccc gagcaaactg cgactactgg cgattaccgt gtcgtattta
     3181 aaaccgggga ctatttcaag aaacaaaatc ttgaaagctt cttccctgag attcccgttg
     3241 aatttcatat taataaagtg aacgagcatt atcatgtgcc tttgttactc agccaatatg
     3301 ggtattcaac ctatcgtggc agttaatgcg taaatatgta aaagctgatc tctgctactg
     3361 ttagggaggt gactccctga cagattaaca gtaaacagct cttgttggtt aacgacaaaa
     3421 aagtgtgatg gcttatgaaa aagaagcaat ttttaaaaga atcagatgtt acggccgagt
     3481 cggtattctt tatgaagcgt cggcaggtgt taaaagcact gggcatcagc gcagctgcat
     3541 tttctttgcc tcacgctgcg catgccgatc tgcttagctg gtttaaaggg aacgatcgtc
     3601 cgcccgcccc cgccggaaaa ccgctggagt tcagcaagcc tgccgcctag caaaataacc
     3661 tgccactgac gccagcagat aaagtctccg gttataacaa cttctatgaa ttcgggctgg
     3721 ataaagacga tcccgcggct aatgctggta gcctgaaaac cgatccatgg acactgaaaa
     3781 tcagcggcga agtggcaaaa ccattgaccc tcgatcatga tgatttaacc cgtcgcttcc
     3841 cgctggaaga gcgtatttat cgtatgcgct gcgtggaagc gtggtcgatg gtggtgccgt
     3901 ggattggttt tccgctacac aaattgctgg cgcttgccga acccaccagc aatgcgaagt
     3961 atgtcgcttt cgaaacgatt tatgcgccgg aacagatgcc tggccagcag gaccgcttta
     4021 tcggcggcgg gctgaaatat ccttatgtcg aaggattgcg tctcgacgaa gcaatgcatc
     4081 cgctcacact gatgaccgta ggtgtttatg gcaaggcgtt accaccgcaa aatggcgcgc
     4141 cggtgcgact gattgtgccg tggaaatatg gctttaaagg gattaaatcg atcgtcagta
     4201 ttaagctgac ccgcgagcgt ccgccaacca cctggaatct ggcagcgcct gacgaatacg
     4261 gtttttacgc caacgttaat ccgcatgttg atcacccgcg ctggtcacag gctaccgaac
     4321 gatttattgg ttcaggcggc attctcgatg ttcagcgcca gccaacacta ctgtttaatg
     4381 gttacgccga ccaggtggca tcgctgtatc gtggcctgga tttgcgggag aatttctaaa
     4441 tgcgtctgac agcaaaacaa gtgatatggc tgaaagtttg cctgcatctt gccggattgt
     4501 tgccgtttct ctggctggtc tgggcgatca atcacggtgg actgggtgcc gatccggtga
     4561 aagatattca gcattttacc ggtcgcactg cactgaaatt tttgcttgcg gccttgttaa
     4621 tcacacctct ggcacgctac gcaaaacagc cgttattgat acgcactcgc cgcctgttag
     4681 gcttatggtg ctttgcctgg gcgacactgc atttaaccag ttacgcattg ctggagttgg
     4741 gcgtgaataa tctggcgtta ttaggtaagg agttaattac ccggccttat ttaacgttag
     4801 gaattatcag ctgggttatt ttgcttgctt tagcgttcac ttcaacccag tcgatgcagc
     4861 gaaaactcgg caaacactgg caacagttgc ataacttcgt ctatcttgtc gcgatcctgg
     4921 ccccaataca ttatctgtgg tcagtgaaga ttatttcacc gcagccactc atctacgccg
     4981 ggctggctgt actgctttta gccttacggt ataagaagtt gctttccctt ttcaaccggt
     5041 tacgcaagca ggcccacaac aaattatctc tataacttca tattgccgac aaacttcgcg
     5101 ttatcccggc cggatgcggc gcgagcgccc tatccggccc acacagatcg catcaagtta
     5161 tgtttcgttt gtcatcaacc tttcatcacc agaataattt attttatttc aataagatag
     5221 ttcaatacac ctgagaatac catgctctca tttcctaatg agttgttgca ttttttcata
     5281 tgttacaata taacattaca cataatgtac attaactttg gaggaaattg ttttggcgat
     5341 tcgtcttcat aaactggctg ttgctttagg tgtctttatt gttagcgctc ctgccttttc
     5401 gcatggtcat cattctcatg gcaaaccctt aacagaggtc gaacaaaaag ctgctaatgg
     5461 tgtttttgat gataccaatg tacaaaaccg aacgctcagt gactgggatg gagtctggca
     5521 atccgtttac cctttactgc aaagtggcaa acttgatccc gtctttcaga agaaagcgga
     5581 tgcagataaa accaaaacat ttgctgaaat taaagattat tatcgcaaag gttatccgac
     5641 agatatcgaa atgattggca ttgaggacgg cattgtcgaa ttccatagaa ataatgaaac
     5701 aacatcctgt aaatatgatt acgatggata caaaatactc acctataaat caggcaagaa
     5761 aggcgttcgt tacttattcg aatgtaaaga tcctgaaagc aaagccccta aatatataca
     5821 atttagcgat catataattg caccacgaaa atcatctcat tttcacattt ttatgggtaa
     5881 tgattcccag caatctttat tgaatgaaat ggaaaactgg ccaacgtatt atccctatca
     5941 gttgagtagc gaagaagtgg tcgaggagat gatgtctcat tgattttttt gcgctgactt
     6001 cagttattta acagaacagt gcctggtagt aacaacaata atttaccaag acaaataagt
     6061 aagacaaact cagacacgct catacttgag tcgtgtcttt tttattatgt ctgtagaaaa
     6121 aaactgagaa aaaggctaac aataaataca caactcagcc atacagactg gcagcgcaaa
     6181 tgtttccagg agcaagtcat ctgtcatggt aaagctaacc agaaatatag gttaattttg
     6241 gtgtttaaac cttttttact tgttagtatg atacaaaggc tttcaaaaaa gctgggaaat
     6301 tcaacaactc ttggaaatta tggactttgt cccgcggctt taatctgacg gccgcgttcc
     6361 ttttttggct caattattga ccccttctct acgcaacttc agttcccgcc agcaactttg
     6421 cggcagtttc ataggatcaa tgtctaaaag aataatggtg accgataaga aaacgactga
     6481 ataactgtag attttcgctc gaaaccttcc tgtcagctcc atagcgaatc aagcgctgaa
     6541 tgtcacagta tcgaacagaa aacagtgacg atctaaccct tcaagaatat tctacgattg
     6601 ttctgtttag gaaaagcaag gcgggaagtc gggagataag tcattgataa aatggcggag
     6661 agagggggat ttgaaccccc ggtagagttg cccctactcc ggttttcgag accgatgata
     6721 cagagtcgaa aaatcaaaga gttaaaaatt tttcttggaa aaaatgtctt aaaatcgtac
     6781 acgaaaaatc aatgaattac ataacattaa agaagaatat tccaagcctt cttgagtcca
     6841 ccacctgttt tcaggtgtca ttcagacgtg atttgcccca gattgggaaa gcacgctttt
     6901 ttctcagctt attccgtttt gcctgtgtgt tgaataattc cactgcagtt atataaaatc
     6961 ggtaacggct ggaaatcatt caatactcgc actatcgaaa gtttaccagc caaccgcagc
     7021 acgtcctgca tacgtcgtgt ctgcggtttt tctttttcgc ttacattgtg tctggttctt
     7081 ccggccactc aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact
     7141 tcttccaggc ttccagcaac gagttttctt cctccgttgc gatctccaga tctacagcat
     7201 cctgaagtgg cgcaatatgc tcactgaatc cctggatgta gaactgtgtg gtgacggtct
     7261 tccagccatt cggctcctgc tgtatcgaag cataccaggc tatttcaata tcgctatgct
     7321 gcggcagcat ttaacccctt gtaattcatc gccataattg atttaattca caaataaaac
     7381 tataatatgg taaaattaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca
     7441 cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct tgcatgggga
     7501 tgtcctacaa aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg
     7561 gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct
     7621 ttaatggatt tgaacgaagc tagcctgaac gcggcatcta caagggctgg ggaatcaaaa
     7681 attaaacata aaattagcca tgatgttttt gaaccttatc ctgcggcgtt acatggtcaa
     7741 tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa
     7801 agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgatg atggaactct atacggagcc
     7861 acaattcttg gcgatggagt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac
     7921 aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc
     7981 tcagagcatt tcgagaatgt taaaaccaag gttcaaggta ctgtagtaat gttttccgct
     8041 tcagggaaaa aatagcatcc aaccgcagca cgttcttgct taagacgtgc tacggtttcc
     8101 tttacgattt ggggttgcga ctttacctct attgataatg cattccggca gaacgttcaa
     8161 atatgaacgt acgatattta actaaccgaa aaacaaaata tagcacaggc gagacattta
     8221 ccatctaaga aaatagtatc gtttttttac agttaaatca acatcacttc cttaaaatga
     8281 aaacaataaa tttcaatcct gaaacatttt atttcagctt attattcccc agtatcacat
     8341 tttatcagga tatccttctg cgaggttatt atgcttcctg taaataatcc ccccctatcc
     8401 actggaaacg tctcttttta cagaactaca tcaatcgaca atgttcacaa taattatctc
     8461 tccgaatggg ttgaatggac taaaaacagc atttccggag aaaacaggga aactgctttt
     8521 acccggctcc aattatgtct ggagaacagt gaaacatcgt tggacttatc ttgtttaggt
     8581 ctcagatctc taccacgatt gcctgacaat cttgatgaaa ttaatgtaag caataaccaa
     8641 ctatcaatgc tccccgagct accaagggca ttgaaagagc tgaatgcaag cagtaatcaa
     8701 ttatctgcac ttcctgaatt accagtgtcg ctggaatata taaatgtgag tgataaccat
     8761 ttgttcgcac ttcctgaatt acctgcgtca ctagaatata ttaatgtaag tgacaatcac
     8821 ctgtctgtac ttccgaggtt accaatgtca ttggaattac ttgatgcagc cagaaatgct
     8881 ttggaagtaa taccagattt tccagaaaga gatgatcata ttataagaat attctggctt
     8941 aatcagaacc ggatcacggc aattccggaa agcatacttg gcctcagttc tgatagcgtt
     9001 gtcaatctta gagaaaatca actatctccc agaataatgc aaactttgtt acaacaaacc
     9061 gcccaaccgg actaccacgg cccacggatt tacttctcca tgagtgacgg acaacagaat
     9121 acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct
     9181 gatgtatcac agatatggca tgcttttgaa catgaagagc a
//
LOCUS       DAJTNP010000135         9194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTNP010000135 DAJTNP010000000
VERSION     DAJTNP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..904)
                     /locus_tag="OO160_003613"
     CDS             complement(<1..904)
                     /locus_tag="OO160_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5525582.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO160_003614"
     CDS             964..1242
                     /locus_tag="OO160_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525583.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO160_003615"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO160_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5525584.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO160_003616"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO160_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5525585.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO160_003617"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO160_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5525586.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO160_003618"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO160_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5525587.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="OO160_003619"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="OO160_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="OO160_003620"
     CDS             complement(4922..5452)
                     /locus_tag="OO160_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5525588.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO160_003621"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO160_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5525589.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="OO160_003622"
     CDS             complement(6421..7644)
                     /locus_tag="OO160_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525590.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9194)
                     /locus_tag="OO160_003623"
     CDS             complement(8225..>9194)
                     /locus_tag="OO160_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5525591.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2681 a         1828 c         1924 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc tgcg
//
LOCUS       DAJTNP010000136         9188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTNP010000136 DAJTNP010000000
VERSION     DAJTNP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO160_003624"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO160_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5525592.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO160_003625"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO160_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5525593.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO160_003626"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO160_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5525594.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO160_003627"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO160_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5525595.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO160_003628"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO160_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5525596.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO160_003629"
     CDS             complement(6031..6360)
                     /locus_tag="OO160_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5525597.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO160_003630"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO160_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5525598.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO160_003631"
     CDS             7998..8171
                     /locus_tag="OO160_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5525599.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..>9188)
                     /gene="kch"
                     /locus_tag="OO160_003632"
     CDS             complement(8226..>9188)
                     /gene="kch"
                     /locus_tag="OO160_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5525600.1"
                     /translation="IALIYTLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTI
                     FAFISFTTLLFYSTYGALYLSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESAR
                     LFTISVIISGITVFATSMTSIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAIN
                     TILQLNQRGQNVTVISNLPEDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAI
                     LALSDNDADNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEIL
                     ARVLNGEEINNDMLVSMLLNSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2370 a         2172 c         2307 g         2339 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctat
//
LOCUS       DAJTNP010000137         9184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTNP010000137 DAJTNP010000000
VERSION     DAJTNP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..923
                     /locus_tag="OO160_003633"
     CDS             <1..923
                     /locus_tag="OO160_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5525601.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO160_003634"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO160_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5525602.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO160_003635"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO160_003635"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5525603.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO160_003636"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO160_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5525604.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO160_003637"
     CDS             4446..4805
                     /locus_tag="OO160_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5525605.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO160_003638"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO160_003638"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5525606.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="OO160_003639"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="OO160_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5525607.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="OO160_003640"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="OO160_003640"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5525608.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2101 a         2223 c         2481 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg ccgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgcagctg agaaaaagcg
     9181 aagc
//
LOCUS       DAJTNP010000138         9179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTNP010000138 DAJTNP010000000
VERSION     DAJTNP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..144
                     /locus_tag="OO160_003641"
     CDS             <1..144
                     /locus_tag="OO160_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HCR5525609.1"
                     /translation="HLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQN
                     PQR"
     gene            203..859
                     /gene="pdxH"
                     /locus_tag="OO160_003642"
     CDS             203..859
                     /gene="pdxH"
                     /locus_tag="OO160_003642"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5525610.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            988..2262
                     /gene="tyrS"
                     /locus_tag="OO160_003643"
     CDS             988..2262
                     /gene="tyrS"
                     /locus_tag="OO160_003643"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5525611.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2321..3184
                     /gene="pdxY"
                     /locus_tag="OO160_003644"
     CDS             2321..3184
                     /gene="pdxY"
                     /locus_tag="OO160_003644"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5525612.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3228..3833)
                     /gene="gstA"
                     /locus_tag="OO160_003645"
     CDS             complement(3228..3833)
                     /gene="gstA"
                     /locus_tag="OO160_003645"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5525613.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3939..5441)
                     /gene="dtpA"
                     /locus_tag="OO160_003646"
     CDS             complement(3939..5441)
                     /gene="dtpA"
                     /locus_tag="OO160_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5525614.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6052..6687)
                     /gene="nth"
                     /locus_tag="OO160_003647"
     CDS             complement(6052..6687)
                     /gene="nth"
                     /locus_tag="OO160_003647"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5525615.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6687..7382)
                     /gene="rsxE"
                     /locus_tag="OO160_003648"
     CDS             complement(6687..7382)
                     /gene="rsxE"
                     /locus_tag="OO160_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5525616.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7386..8006)
                     /gene="rsxG"
                     /locus_tag="OO160_003649"
     CDS             complement(7386..8006)
                     /gene="rsxG"
                     /locus_tag="OO160_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5525617.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8010..9068)
                     /gene="rsxD"
                     /locus_tag="OO160_003650"
     CDS             complement(8010..9068)
                     /gene="rsxD"
                     /locus_tag="OO160_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5525618.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2347 a         2303 c         2331 g         2198 t
ORIGIN      
        1 catctcaaac agggcctttc agcctctggc gcgcgttact ccgacgggat ctatgttttc
       61 tggtcgaaag gtgaggaagc gactgtctat aaacgcgacc gcatcgtctt gaataactgt
      121 cagttacaaa atccacagcg ttgagatttt tccaggggcg gcgcacaata gcgtcaccca
      181 ctgacaatcc gtaaagaaaa ccatgtctga taacgacgaa ttgcagcaaa tcgcgcatct
      241 gcgccgtgaa tacaccaaag gcgggttacg ccgccgcgat cttcccgccg atccattaac
      301 cctttttgaa cgttggctct ctcaggcttg tgaagccaaa ctggcggacc ctaccgcgat
      361 ggtggtcgct accgtggatg aacatggtca gccttatcag cgcatcgttt tactcaaaca
      421 ttacgacgaa aaaggcatgg tgttttacac caacctcggt agccgtaaag cacatcaaat
      481 tgaaaataat ccgcgcgtta gcctgctgtt cccgtggcat acccttgaac gccaggtgat
      541 ggtgatcggt aaagcagaac gactttcgac tctcgaagtg atgaaatatt ttcatagccg
      601 cccgcgtgat agccagattg gtgcatgggt ttcgaagcag tccagtcgca tttctgcccg
      661 cggtatcctt gaaagtaaat tcctggagct gaagcagaag tttcaacagg gcgaagtgcc
      721 attgccgagc ttttggggcg gttttcgcgt cagccttgaa cagattgagt tctggcaggg
      781 cggtgagcat cgcctgcatg accgcttttt gtaccagcgt gaaaatgatg cgtggaagat
      841 tgatcgtctt gcaccctgaa aagatgcaaa aatcttgctt taatcgctgg tactcctgat
      901 tctggcactt tattctatgt ctctttcgca tctggcgaaa agtcgtgtac cggcaaaggt
      961 gcagtcgtta tatacatgga gattttgatg gcaagcagta acttgattaa acaattgcaa
     1021 gagcgggggc tggtagccca ggtgacggac gaggaagcgt tagcagagcg actggcgcaa
     1081 ggcccgatcg cgctctattg cggcttcgat cctaccgctg acagcttgca tttggggcat
     1141 cttgttccat tgttatgcct gaaacgcttc cagcaggcgg gccacaagcc ggttgcgctg
     1201 gtaggcggcg cgacgggtct gattggcgac ccgagcttca aagctgccga gcgtaagctg
     1261 aacaccgaag aaactgttca ggagtgggtg gacaaaatcc gtaagcaggt tgccccgttc
     1321 ctcgatttcg actgtggaga aaactctgct atcgcggcga acaactatga ctggttcggc
     1381 aatatgaatg tgctgacctt cctgcgcgat attggcaaac acttctccgt taaccagatg
     1441 atcaacaaag aagcggttaa gcagcgtctc aaccgtgaag atcaggggat ttcgttcact
     1501 gagttttcct acaacctgtt gcagggttat gacttcgcct gtctgaacaa acagtacgat
     1561 gtggtgctgc aaattggtgg ttctgaccag tggggtaaca tcacttctgg tatcgacctg
     1621 acccgtcgtc tgcatcagaa tcaggtgttt ggcctgaccg ttccgctgat caccaaagca
     1681 gacggcacca aatttggtaa aactgaaggc ggcgcagtct ggttggatcc gaagaaaacc
     1741 agcccgtaca aattctacca gttctggatc aacactgcgg atgccgacgt ttaccgcttc
     1801 ctgaagttct tcacctttat gagcattgaa gagatcaacg ccctagaaga agaagataaa
     1861 aacagcggta aagcaccgcg cgcccagtat gtactggcgg agcaggtgac tcgtctggtt
     1921 cacggtgaag aaggtttaca ggcggcaaaa cgtattaccg aatgcctgtt cagcggttct
     1981 ttgagtgcgc tgagtgaagc ggacttcgaa cagctggcgc aggacggcgt accgatggtt
     2041 gagatggaaa agggcgcaga cctgatgcag gcgctggtcg attctgaact gcaaccttcc
     2101 cgtggtcagg cgcgtaaaac tatcgcctcc aatgccatca ccattaacgg tgaaaaacag
     2161 tccgatcctg aatacttctt taaagaagaa gatcgtctgt ttggtcgttt taccttactg
     2221 cgtcgcggca aaaagaatta ctgtctgatt tgctggaaat aatgcattaa gtggaaaggg
     2281 ggagtgagaa atcactcccc ctggttttta tacagggaac atgatgaaaa atattctcgc
     2341 tatccagtct cacgttgttt atggtcatgc gggtaacagt gcggcagagt ttccgatgcg
     2401 ccgcctgggc gcgaacgtct ggccgctgaa caccgttcaa ttttctaatc acacccaata
     2461 cggcaaatgg actggctgcg tgatgccgcc cagccattta accgaaattg tgcaaggcat
     2521 tgccgccatt gataaattac acacctgtga tgccgtatta agtggctatc tgggatcggc
     2581 ggagcagggt gaacatatcc tcggtatcgt ccgtcaggtg aaagcagcga atccgcaggc
     2641 gaaatatttt tgcgatccgg taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg
     2701 tgtcgcagag tttcatgtgc ggcacggttt gcctgccagc gatatcattg cgccgaatct
     2761 ggttgagctg gaaatactct gtgagcatgc ggtaaataac gtcgaagaag cggttctggc
     2821 agcgcgcgaa ctcattgagc aagggccaca aattgtgttg gttaaacacc tggcgcgagc
     2881 tggctacagc cgtgaccgtt ttgaaatgct gctggtcacc gccgatgaag tctggcatat
     2941 cagccgtcca ctggtggatt ttggtatgcg ccagccggta ggtgttggtg atgtgacgag
     3001 cggtttactg ctggtgaaac tgcttcaggg ggcaacgctg caggaggccc tggaacatgt
     3061 gaccgctgca gtctacgaaa tcatggtgac caccaaagca atgcaggaat atgagctgca
     3121 agtggtggct gctcaggatc gtattgccaa accagaacat tacttcagcg caacaaagct
     3181 ctgaaatatc tcatttcggc ccacaacgac ctgtgggccg atggctatta ctttaagcct
     3241 tccgctgaca gcgcgtcttg tacttccgga cgttcagcca tacgttgcat aaatgccgca
     3301 atgtgctcta acccttccag attcagtttc accgcgtatg cccagcgcag aaccgtaaac
     3361 agataggcat cagcaattgt aaatctttgc ccgcagatcc agtgctcatc cttcagtgcc
     3421 tcgttcacat attgcagctt cttatccagc tgcgcgcgaa ctgtcggttt gtactcttcc
     3481 ggtgtatctg ggcgaaacag aggtgtgaaa cctttatgca gctcggtggc gatgtaattc
     3541 agccattcga tggttttata gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg
     3601 gggacgctgt cggcaagata ctgcataatc gcaacgcctt ccgtcagcaa agtaccatcg
     3661 tccagcagca atgcaggcac ctgcccctta gggttaacgg aaaagtaatc gtcaccgttt
     3721 tcgagacgtt ttttcattaa atccacactg acgagcgtaa aatcctttcc gctctcacgc
     3781 agggtgatat gggaagcgag agagcaggcg cccggtttgt agaacaattt catcggtaac
     3841 tccttttgtc tgtggtttaa agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa
     3901 cgaaaagtta gcggcttgta aaagagtttc cctgaatttt acgctacagt agctttcgcc
     3961 gctttgtctg cagcgtcatc ctgcgtcatg cggtgcagtt taggcgcggt cagcagcatc
     4021 agtactgcaa taacggcagt agcgacacca atctgcaaga atacgcgacc atagacttcc
     4081 agtgacatca gcgggtcggt aacgttatcc ggcacagcca tcatacccgc aacataacca
     4141 ccaatcaggt ttgcaccggc agtggtcagg aaccagctac ccataatgaa gcccatcaga
     4201 cgctgcggaa cgagttgtgc aaccattgcc agacccagac cagagatcat cagttccccg
     4261 atgctctgca ggccatagct tgcgaccagc cagctgacag acacgatacc tgcgtcagac
     4321 gcgaatttcg cacccagcgg cagaatcagg aacgcaccag aacacatcac catgccgatt
     4381 gcaaacttgg ttggcatcgg cagggtatca cccatcttgt tatagatagc ggccagaatc
     4441 ggactaccga tgatgatcca gaacgggttc agtgcctgat actgctcagg ttctacggcc
     4501 agacccagaa tggagtgctc aacgttacga atcgcaaaga agttcagtga cgttggcatc
     4561 tggctgtaca gcacgaagaa gatgattgct tcgagcatca ggatgaaggc aacgatcatt
     4621 ttacgacgcg cagcaccttt catcgcgaaa gcttctttac cgaagataac cacaataccg
     4681 aaggcaacaa cgcccagcgc catacgcgca acttcctgat tgtgcagtag ccaggtggca
     4741 acagcgatca gcgccacaac accaataatg gtcagcaaca ggttacggta gttgactggt
     4801 tcaaagtctg gttttgaacc gtactgttta acccagcgtt ggcagaaggc gaagttaacg
     4861 atagtgatca gcaggcctac aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg
     4921 gccagccacg gggtggctat catagagaag aaagagccga tgttgacgga catgtagtac
     4981 atggtgaatg caccgtccag acgtgggtcg tttttctcat agcatgtaga aagcagagaa
     5041 gacgggttag ctttaaacag gccgttaccg accgcaatag ccgccatacc catataaaca
     5101 ataccggcgt cgtgaccaga ccaggcaacc agagcataac caatcgccag cacaatagcg
     5161 ccgagcataa ttacgcgttt agtacccagt accttgtcac ctaaccagcc gccgatagcg
     5221 accagaccat aaaccagggc actaaaggaa gagaaaaggg tgattgaatc cgcttcagac
     5281 atacccagtt gtttaaccag gtaaacagcc ataattcctt gtaggccgta ataaccaaaa
     5341 cgttcccata actcaatcga gaagatgaga tagaacgcct tcggttgttt gaaagcgttc
     5401 aaactgacgc tttcagttgg tttttggttt gcagtggaca cttttacctc tttttttaca
     5461 tcccatatta acgggggtgc tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta
     5521 tagtgggagg ggaaacggca ggtaatgttc actatcttgc actttcagac aagtcctttg
     5581 taataatctg ttacatataa ctgaggcggg gaattcaacc attccgggaa atgttatcca
     5641 gcgttaaatt tttcaaaaaa cgtaacggca gattttttca ctgcattgag agggggatga
     5701 tggcttggcg gcgatatgtt ctgctattta gcgtttttcc tagtgagata gtccagtttc
     5761 tgaaaaatag ccagtgtaat gttttgcagg tcaatatttg ttgcgtgtgt aaatttaaat
     5821 cattttaacc tgattcttgc aatgttttta cattgccaca gcatcgtgat cttgatcacg
     5881 aatgaataga aaatttcgct attaaaaaaa caattaacct gcatttttgg ttatccgcaa
     5941 acggattacc tgatgataac gctaagcaat ggcatcattg acgttaaaaa gatgttttaa
     6001 aagaggataa agaaaggtta tcaatggggt aatcggtgtt accccttttc ttcagatgtc
     6061 aactttctct ttgtattcac aaagatcttc aataatacaa gagccacagc ggggcttgcg
     6121 ggcaatgcag gtataacgcc cgtgcaggat caaccaatgg tggcagtcga ctttaaactc
     6181 tgccggaacc actttcagta gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa
     6241 ttgagtacga ttacaaacgc ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa
     6301 tgcagtgttt aatacgacgt tggctgtttt acgacctacg ccgggcaggg cttcaagcgc
     6361 agcacgatct tccggaacct cgccattatg ttgttccagt aatatacggc aggttttgat
     6421 gatattttct gctttgctgt tataaaggcc aatcgttttg atataggttt tcaccccttc
     6481 aacgcctagt tcaagcatcg ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc
     6541 cttattaaca ctgatatcgg tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa
     6601 aggcgaactg aaattaagct cggtggtggg atgaggattg ttctcacgca ggcgagtgag
     6661 gatctccaga cgttttgctt tgttcatcag acattccctg tttcaccgtt tggcaatgca
     6721 cgttcggcag ctgcttcagc acggcgcttt ttcattcttt catcaatcag gtattttcct
     6781 gccagcatca gtcccaagcc aataaatgca cctggtggca gcatcgccag caggaaaggg
     6841 gagtcggtgt ggaaaatctc cacgcgtaat acttttgccc agctacctaa cagcgcatct
     6901 gcaccgtcaa acaacgtgcc attgccgata atttcgcgta gtgaacccag cacgaacatg
     6961 gcgcaggttg cgcccgtacc aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt
     7021 ttggcggcga aggcttcagc gcggcccaca acgatacagt tagtgacaat cagcggaata
     7081 aaaatcccta atgattgata caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg
     7141 ctgaccaccg aggcgatgat catcacgtaa atgggaatgc ggatctcggc tggcgtccag
     7201 tgacgcagcg tcgaaatggt caggttggtc agcgtcagta ccagcgtagt cgcaagtcct
     7261 aaacccagag cgttagtggc agtggatgtg accgccaata gcggacaaag gccgagcagt
     7321 tggaccagtg cagagttgtt tttccacaac ccctgaacaa taacgtcttt aatttcgctc
     7381 acgctttatt ctccacaggc aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac
     7441 aatccggcgc gttttaccgc attaaccacc gcgcggggag taatcgtcgc gccggtgaac
     7501 tggtcgaaat caccaccatc tttcttcacc gcccagtgcg catcatccgc accactgatt
     7561 ttttttcccg caaaatgggt gagccagtca gaaaggcgca attcgatttt atcgccaagc
     7621 cctggcgttt cgtggtgctc tgtcacgcgc gtgccaagta ccgtgccatt aaaatcggct
     7681 cccaccagca gctgaatcgc accggaatag ccatctggcg cggttgcttc cagaacggcg
     7741 gctaccggtt tgtcatcctg tttggcgatg taaacccgat gctcaccttt acctaactct
     7801 ggcgcagtta ccagatagca actctgtgcg agcgcattgt tatagcgttc ggctggcagc
     7861 acctgatcaa ataacgtctt ttgttgcaga ctggcctgtt cagcaattgt cgttttggtc
     7921 atctggttga tggccgcagt taaccctgta gaacccgctg caaatagtgc cagtgtaatg
     7981 ccgtgttttc ggatagtttt cagcatggtt taccctttgc gatggccgta gacgcgcgga
     8041 cgcgtgtagt aatcgatcag aggaaccgtg atgttcgcca gcaggacggc aaaagccacg
     8101 ccgtcaggat agccgccgaa actgcggatc aaccagacta ataagcccgc cagcgcgccg
     8161 aacatcagcc gaccacgatt ggtcgtagaa gcagtaactg ggtcagtcaa aataaagaat
     8221 gcgccgagca tagtcgcacc agacagcaga tgaatttgcg gtgctgccag tgtttctggt
     8281 gagaacaacc agcccaacgt tgcgcataac gccagcgtta ctaagaagct gaggggaata
     8341 tgccagcgaa tcgctttctg ccatagcaac cacacgccac cagccagcca ggcgagattt
     8401 acccattgcc aaccagcgcc cgccagaata ccgctgtaga tcggatattg cataatctgt
     8461 tcaaccgaat gacctgcgcg gacagaggtt ttgaatgtat ccagcggtgt cgcctgacta
     8521 atgccatcaa tacctaagcg tagcgtattc atatcaccac cactggcggt atgaccgctg
     8581 aaaataacct gtatggcgtc gataaaacca gggatgttga ccgcaatttc atgtggcggt
     8641 aaccagctgg tcatctgcac cgggaaggag atcagtaaga ccacataacc aatcattgcc
     8701 ggattaaacg ggttttgccc cagaccgcca tacaactgtt tagcgatgat caccgcaaac
     8761 accgtaccca gtacgaccat ccaccatggc gctagggggg gaatacttac cgccagcaat
     8821 aagcctgtca gcaatgctga gttatctttc aacgttgcgg ctaccgactg cttgcgtagt
     8881 ttgagtacga gagcttcggc taacagagca ctgaccgacg ccaacaggat ctgaacgaga
     8941 gtaccccaac caaaaaaccg cagttgcgct gcgattcctg gcacggctgc gagcaacacc
     9001 aacagcataa tgcgcgatgt ctggcgctgg ttatgggtat aaggggagct agctattctg
     9061 aataccattt agtcctcgtt tacaaccttt tgctgggcgg cttttttggc ctgaacgcgg
     9121 gcaatagccg cggcaactgc ggctttgcgc ggatcgacct gttcttctgg ttccgcatt
//
LOCUS       DAJTNP010000139         9167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTNP010000139 DAJTNP010000000
VERSION     DAJTNP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..592)
                     /locus_tag="OO160_003651"
     CDS             complement(<1..592)
                     /locus_tag="OO160_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5525619.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(659..1384)
                     /gene="fimC"
                     /locus_tag="OO160_003652"
     CDS             complement(659..1384)
                     /gene="fimC"
                     /locus_tag="OO160_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5525620.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1421..1960)
                     /locus_tag="OO160_003653"
     CDS             complement(1421..1960)
                     /locus_tag="OO160_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5525621.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2025..2573)
                     /gene="fimA"
                     /locus_tag="OO160_003654"
     CDS             complement(2025..2573)
                     /gene="fimA"
                     /locus_tag="OO160_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5525622.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3054..3650)
                     /gene="fimE"
                     /locus_tag="OO160_003655"
     CDS             complement(3054..3650)
                     /gene="fimE"
                     /locus_tag="OO160_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5525623.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4128..4730)
                     /gene="fimB"
                     /locus_tag="OO160_003656"
                     /pseudo
     CDS             complement(4128..4730)
                     /gene="fimB"
                     /locus_tag="OO160_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6184..6900
                     /gene="nanC"
                     /locus_tag="OO160_003657"
     CDS             6184..6900
                     /gene="nanC"
                     /locus_tag="OO160_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5525624.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6920..8026
                     /gene="nanM"
                     /locus_tag="OO160_003658"
     CDS             6920..8026
                     /gene="nanM"
                     /locus_tag="OO160_003658"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5525625.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8091..9071
                     /locus_tag="OO160_003659"
     CDS             8091..9071
                     /locus_tag="OO160_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5525626.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2686 a         1995 c         1924 g         2562 t
ORIGIN      
        1 tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata ccgggatccc
       61 ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga gggatcgtca
      121 ggttcagtcg ctgctgacca acatccagat gcgcagtagc gtcctggacc attgtggtta
      181 atggcacaca ggcatcatcc gccagcagat tcataccggc gacagaagcc gtattcagcc
      241 ccatactggc gagttgcgcg cgtgtcaggc agggaacaat cccttgttca ctgtcgcccg
      301 tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata tcgacgcgat
      361 acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcggcc acagcctggg
      421 gatcatccgc taaaaagcgc agattaaaat agaggtcggc agatgacaaa ggtgcctgtg
      481 cggcaaaagc acaggcgaca acgagtcgga caaaaaaacc agccaaacga tgcttacgaa
      541 tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac attattcctc
      601 cggtttaaaa tcacccggca gtcaattctt tttattcagg cgaaaaattc cccctgcgtt
      661 attccattac gcccgtcatt ttgggggtaa gtgcgccata atcatttatt gttcggtaag
      721 taatattgct tcctgcatca gaaggcaatt taaccgtgct ttcgcccatt ggaggcacca
      781 atgcattttc aagaacccgg gttccggcat tcaactctgt taccgtcagg taatagggtg
      841 tcgggttaat cagcgtcaga gaattcgcgc tacgacgaaa tcttaatttt tctgcggcct
      901 gatcgggtgg caacgctaat ttagccgggc gatagtacag tttaatgcgg ctgataattg
      961 cgagctgtag catattctca gtcaattttg atttatccat tgacggaatc gctttaacgt
     1021 tcatccagaa taaactttcc cggtcctgtg gcaattggtt atttgttgca tcaagaatac
     1081 gtaaggtatt ctcttttttt cccttcatcg caaacagagg aggcgtcacg ataaaacgac
     1141 catcctttac accatcggca ttttccaccc atgattgaat taaataggta ctattttcat
     1201 cattatttgt cacggcaagt tgcacttgtt tttgccctgc cggataaatt acgcgagtcg
     1261 cacctaaggc cactcctgct tcagcgcgtc cggcaaccat cattgccatg aacatcagga
     1321 tacctgccag caagcagaat gttatttcct gcgatttcct tacattgacg tttttattac
     1381 tcactgtcct gttcctgtta tcacattatc tactgaacaa ttactgatag gttaaagaga
     1441 accaggcctg ggcattggcg atgccgccag taacccgacg cccggtagca cgatatttgg
     1501 cgatgaaatg tagcgaagta gagcctgagt aaagccgttt ccagtttgct ggaggacgat
     1561 taatcggtac gaggtttcct tcatcatcaa acaacgctac accaatattg gaggctatcc
     1621 ctggcccctc tcccacggag agcacatccg gatttttacc atccgcgaca ccgtgaaacg
     1681 ccacacccac acgttcactc accaccgtgc tacattcccg taaatgaata acaaaaggca
     1741 ccggtgcgct atcttcccct gccgcatgaa accggttact gctgatttgc cccatattga
     1801 ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg acgccctgaa
     1861 attgcatatt tccgccgggc agcgtggtat tccatttatt tccggccaga gcaaacattg
     1921 gcagcaacga cgctaacaga aataatctct tccttttcat cgttattttt atcgcacaag
     1981 ggtgggcatc cctgcccgta atgacgtcct tgaacctggg taggttattg atactgaacc
     2041 ttgaaggttg catccgcatt agctgcaccc ggggttgcct cgccgattgc ataataacgc
     2101 gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg tttgtgcact aaatgtcgca
     2161 ccgtccagcg tcagagcatt gcctgttctg tctaggatct gcacgccaac gtttgttgca
     2221 ctacctgcag cagaactctg cagagccagt acatcagtac gcgtagcatc aattgccgta
     2281 cctaagaagg caacagcggc ttttgtggca acagtggtat cgcaatcatt cagctgaatg
     2341 ttaaaaccaa cggcagagct ggttgctcca gcctgcttca ggctagcggt acgaacctgt
     2401 cccaactgaa cggtttgatc aacagagcct gcatcaactg cgcaagcggc gttaacaact
     2461 tcccctttaa agtgaatggt cccaccattt accgtcgtag tatcggccag agccgctgcg
     2521 gaactgaggg acagagccga cagaacaacg attgccagag ttttaatttt catgctgctt
     2581 tcctttaaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac tttgttttgt
     2641 caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac atttggggcc
     2701 attttgactc atagaggaaa gcatcgcgca caaacttttt cagtttattt gttggcttaa
     2761 tattctatta ttatctttat ttatagattt ttatattgca tgaggtggtt tttggagaga
     2821 agaatgagga agatgcgtcg agccacagaa acgttagctt tacatatagc ggaggtgatg
     2881 tgaaattaat ttacaagaga aataatttac atatcaaata gttagatgct ttttgtcgtt
     2941 ttttaatatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg gacagtttgg
     3001 ccccaattgt cttgtgttta tttgtttttt aactttatta tcaataagtt aaatcaaacc
     3061 tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc ggcaaaacga
     3121 gcagcattac tggcggtata acgcacagta tggcgaatat ttcgatgccc gagataatcc
     3181 tgaattaaac gggtatctgc accacgctcc gccagttcat aaccgcaagc atgccttaac
     3241 atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcgcg aataatgcga
     3301 taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc gtcggtccgg
     3361 tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc acgctcatca
     3421 aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt tattcgacct
     3481 tcattaaggt caaggtcctg ataatgcaga tcaagcagtt cactaatacg catcccatgc
     3541 cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta acaaaccgcc
     3601 tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac aatagtagta
     3661 ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg tcaaaattag
     3721 cccaatagat aaaaagataa ccacacacga agttatgtgg ctatcagtca attacattaa
     3781 acatcataca aaaataaaat atcactgatg tgacaacatc tattttcatt gatttgattc
     3841 aatataacaa tagattacag aggattaact tccagttact taaaaaataa agaaaaaagt
     3901 gtggaaacag gagaatttaa gtgatgtgta aacttatgaa tgattttgtt tatttatttt
     3961 gaagaacata aatcattaga ttttgtatgg gtatggttaa cttaatgttt attatgataa
     4021 ttaagaatca cacttacaat aacatcaatt aataaatggt taatcatttt tgatatatcg
     4081 taagaataat gtagttttta acaccatccc tggtatctca actatctcta taaaacagca
     4141 tgacgctgtc gtcctctggc tctatcccag atgccgtaaa aacgccctgc attgctggcg
     4201 gtataccaga cagtatgacg aatattgcga tgcccaagat aatcctagat aagtcgcgta
     4261 tctattccca tattcgccaa agcaaaacca cacgaatagc gtaacatgtg cggatgaatc
     4321 tccagtgaca acccggcatt accaccagaa gtcgagataa tatggtaaaa ctgttgccga
     4381 gaaagcggat tccccttacg tgataaaaat acccactcgc tctcagcatg cggataagaa
     4441 gtacggatac tcaaccagtt ttttaaagcc tgaacttctt tgttcaatag cgggtgcgtt
     4501 gttgaaaagc ctttttttaa tcgatggata tatatacact ttgctttaag atcaatatcc
     4561 gaaatcctca atcgacaaat ttcactcgcc cggaaaccat gaataaaaca aagcaaaatc
     4621 agacaataat tacgtgttgc atgaggcccg gtatttgctg ctttaaggag tgactcgatt
     4681 tcactatggg taaggaagtt cctttttttg ttatcagcct tattcctcat cgttttccct
     4741 tataattaca gacgcgcact agctgtgctg ggttagcaat aatccaacat ttttatcctg
     4801 attatgttta taaaagcgaa cgtttgctta ataacttacg taagtggacc agttcttctg
     4861 agcgaactta aatggagtag cgatagttac ttataataac tttgccatac aaacgccatg
     4921 ctcttgcatg ctatgtacct ttatatattt atcaatctga tacgaatcta ataataaccc
     4981 tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt aacaagtaaa
     5041 atcaacataa aaaattcagc aaaatttaat atttgttaaa ctagccgtct caaatatgac
     5101 attattagtt aactgcattt gccacaaaat ggctatttca tggaacgcag taatttctta
     5161 gtggctatta tcatgctaat tcttagatcc ctagcgatta ttcctgcctg gttcgtcagc
     5221 ttaggccatg agattgttac attccttttt atcctggaga tatgggggaa cgcaggcgac
     5281 gctttttcat ctacatccgt tggtaaatat agctttggtc actacgactt aagccttcct
     5341 caatcacagc tattcgccgg gcgatacggc aaacaaacta agtgaaacat catcctgaaa
     5401 acaccaacat caacaagcct ctccagatcg acatcaggag tgaccagtta caagccacaa
     5461 accaagaaac atatttcatg atgagaatta tgctcagcct gatggcgagt aaacccttat
     5521 tttcaccact ctcactgagt tagcttgcaa agctttcttt gtttagattc taaataaatt
     5581 atttataaaa cgtatagtta aaagaaaatc tttagttttt gaagaccacc gcaagtgttc
     5641 gtctggcttc acatggcatc ttcttcttag aaaaagatcg acatattttg tgacgcgaat
     5701 tgcaaatctg gttttgttgt atggattgcg tgatttttga tctggtataa caggtataaa
     5761 gatgcaccac gatagtcaat gagactgggc atctcgtaat ctatggcaaa catcacttca
     5821 gttctttcac atcgggtgat gaaaatgcac ttcagtctga aaggaatatg aaaatgagat
     5881 caacagacac tctattttat gactctgggt aaaatgggtt gagtaagtga tataacttac
     5941 gaatattcaa atcaattaaa aatcagaaga gattttatac tcaggtattt aatctggatc
     6001 tctgtttatt taaataatgt taaaagagaa ttttcacagg agaccttata caaaaaatat
     6061 aaaatacagt tatccgttgc caaagacact ataagcctgg caaaaaaata ttacacaaca
     6121 taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa atgagtgaaa
     6181 tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc gtccccatta
     6241 atttctcagg ctgcgacact ggacgtacgt ggtggatatc gtagtggaag ccacgcctat
     6301 gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag catggaaagt
     6361 aatacctgga ataccattca tgataataaa aaggaaaatg ccgcactcaa tgatgttcag
     6421 gttgaagtta attacgcaat taaacttgat gatcaatgga cggtgcgccc gggaatgtta
     6481 acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc ctgggacgcg
     6541 acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta ccgacaacaa
     6601 gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata tgtcacttac
     6661 catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa acagaatgat
     6721 tatcgctatg caaaccacaa gaaatgggcg acggaaaatg cctttgttct acaataccat
     6781 atgacgcccg atattacgcc atacatagaa tatgactacc ttgaccgcca gggtgtttac
     6841 aacggcagag ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag
     6901 tagacaggag acagtcacaa tgaataaaac tataatggcg cttgctatca tgatggcttc
     6961 atttgccgca aacgcgtctg tattaccgga aactcctgtg ccatttaaaa gtggtaccgg
     7021 agcaattgat aacgacactg tctacattgg tttaggtagc gcaggtacag catggtacaa
     7081 gctagataca caggccaaag ataaaaaatg gactgcgtta gctgcattcc ctggtggacc
     7141 gagagagcaa gcaacctctg catttattga tggcaatctg tatgtgtttg gcggcattgg
     7201 caaaaacagc gagggattga ctcaggtatt taatgacgta cacaaataca accccaaaac
     7261 caatagctgg gttaaattga tgtcgcacgc gccgatgggc atggcgggtc atgtgacttt
     7321 tgtacacaac ggcaaggctt atgttactgg cggtgttaac cagaatatct tcaatggcta
     7381 ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct atagataaaa tcaacgccca
     7441 ctattttgac aaaaaagcag aagattattt cttcaataag tttctgttgt cttttgatcc
     7501 ctcaacacag caatggagtt acgctggcga atcgccctgg tacggaacgg ctggtgcggc
     7561 ggttgtgaat aaaggtgata aaacctggct tattaatggc gaagccaaac caggattgcg
     7621 aacggatgcc gtatttgaac ttgatttcac cggtaataat ttaaaatgga ataagcttga
     7681 tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg gggataagca atgattctct
     7741 tatatttgct ggaggggccg gattcaaagg ttcacgagaa aattaccaga acggtaagaa
     7801 ctatgcgcat gaaggcctga aaaaatcata tagcactgat attcatcttt ggcataacgg
     7861 gaaatgggat aaatcgggtg aattatcgca aggtcgggcc tacggagtat cattgccctg
     7921 gaataatagt ttattaatta ttggcggtga aactgcaggc ggcaaagcgg tgacggattc
     7981 agttttaatt tctgtgaagg ataataaagt cacagtacaa aactaacgct tcagggcccc
     8041 ggtaaagggg ccctgctatc aatttgtcat ttgtattacg cgaaaattat atgaacgcaa
     8101 taatatcacc cgattattac tatgttctta ccgttgctgg tcagtctaat gccatggcgt
     8161 atggcgaagg actgccatta ccggacaggg aagatgcgcc tcattccaga attaaacaat
     8221 tagcgagatt tgcgcatacg catcccggag gcccgccatg tcactttaac gacattattc
     8281 cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat cctctggcaa
     8341 cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca cggaaattac
     8401 tgcccttcat tcctgataat gcagggattc ttatcgttcc gtgttgccgt ggcggatcgg
     8461 cttttaccgc gggcagcgaa gggacatatt cagaacggca cggagccagc catgatgctt
     8521 gtcgttgggg aacggatact ccgctgtacc aggatttagt cagcagaacg cgagccgcac
     8581 tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg gatgcaaggc gaatttgact
     8641 taatgaccag tgactacgcg tcacaccctc aacactttaa tcatatggtt gaagcctttc
     8701 gtagggatct aaaacaatac cattctcagc ttaataatat tactgacgcg ccgtggtttt
     8761 gcggcgatac cacctggtac tggaaagaaa atttcccgca ttcgtatgaa gctatttatg
     8821 gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag caacaaggtg
     8881 aaagaggact gacgaacgcg cctgatgaag acccggacga tttaagcacg ggatattacg
     8941 gttcagcgta ccggtcaccg gagaactgga cgacggcact gcgaagcagt catttcagca
     9001 cggcagcccg tcgggggatt atttctgaca gatttgtaga agcaattttg cagttttggc
     9061 gcgaaaggtg agcggggagt ttattcatgg gggatgtcct tgttcgttct cagagtagac
     9121 aacaaaaaac aaatgagatc aaggtaatat ctcttcactt gcgggct
//
LOCUS       DAJTNP010000140         9134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTNP010000140 DAJTNP010000000
VERSION     DAJTNP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            305..457
                     /gene="hokE"
                     /locus_tag="OO160_003660"
     CDS             305..457
                     /gene="hokE"
                     /locus_tag="OO160_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5525627.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(579..1199)
                     /gene="entD"
                     /locus_tag="OO160_003661"
     CDS             complement(579..1199)
                     /gene="entD"
                     /locus_tag="OO160_003661"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5525628.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="OO160_003662"
     CDS             complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="OO160_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5525629.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3857..5059
                     /gene="fes"
                     /locus_tag="OO160_003663"
     CDS             3857..5059
                     /gene="fes"
                     /locus_tag="OO160_003663"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5525630.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5062..5280
                     /gene="ybdZ"
                     /locus_tag="OO160_003664"
     CDS             5062..5280
                     /gene="ybdZ"
                     /locus_tag="OO160_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5525631.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5277..9122
                     /gene="entF"
                     /locus_tag="OO160_003665"
     CDS             5277..9122
                     /gene="entF"
                     /locus_tag="OO160_003665"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5525632.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAGRTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         1984 a         2416 c         2520 g         2214 t
ORIGIN      
        1 atggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgatttcg
       61 ttttaagagc aatgatgagt tttgccgaca gaagtccgaa acaggactca ctgtataaat
      121 aaacagctat tttgctaagg aagggtaaga taacggcggg tgcctgaagc tttccggttt
      181 caggttaact ctgaggtttg gaaagatgaa gccccaagag atatttctat caatcctggg
      241 gctgccactc cgaacctcaa caactcggat ggtagtccct tcttcgcatg gaggcaatat
      301 aaacatgctg acgaaatatg cccttgtggc agtcatagtg ctgtgtttga cggtgctggg
      361 atttacgctt ctggtcggag actcgctgtg tgagttcacc gtaaaggaac gtaatattga
      421 gtttaaggct gttcttgctt acgaaccgaa gaagtagccg ttgtgcgggg agtaatcccc
      481 gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct ttacactttc gcccgcagtc
      541 agtgatggct gcgggcgaat cgtaccagat gttgtcaatt aatcgtgttg gcacagagtt
      601 atgactattt tttcttttat ctgccagtgc acagcaaaca tctcattctc acgatgaatg
      661 atgacctgct gtttattcca gctaattatc tgatagtcca gaaaacctgc atcagtttgg
      721 atctcacttg ccttaaatgc gctctctttg gcggaaaatg caagtgtcag cgccagagaa
      781 aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg gtgtaataat gttgtctgtc
      841 aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat caacgccaat cggttgacgg
      901 gaaaccacgg ccaatgccgt agccccgcag tggctaatac tgccgtatac ctccgcaggc
      961 cacaccggtt ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcaag
     1021 gcataaacag cagcgatccg tccggctaaa tgctctgttt tacgtttacg tccagcgtgt
     1081 tgcagttgtg cgtagtgcgg cagccagagt aaatcctgct cacaaaaatt cgccggatcg
     1141 aactcaacaa aatgcagcgt atgtccggca aaggggaggg aggtatgcgt agttttcata
     1201 tcgaccatat tcgagactga tgacaaacgc aaaactgcct gatgcgctac gcttatcagg
     1261 cctacgtgat cctgcaatat attgaattgg caagatcttt gtaggccgga taaggcgttc
     1321 acgccgcatc cggcgtgaac gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag
     1381 tgagtattaa tgctcatata ccacgtacgt cccggctcgt tataggtata cgcgccggca
     1441 ccggcgatat agttggctcc tgccagatcg cccgtggtct gggcattacc tgcacgccac
     1501 aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac tgacattctt cgtcacatcc
     1561 caggtcgcgc tcagacccac aatgctgtaa ggactgattt ctttggtttc cgggccaacc
     1621 gctggctgac ctttatagtt gtacttcttc ggctgctgct tgccgtacca ggtgaaggtc
     1681 gtttgcatcg acaaatcttc ccgtgcctgc cagctcagcg ttgagttcaa cgtgtactcc
     1741 gggatgatcg acaaacggtc gcccgtggtt ttgttttcac tcttcagcat ataagtgatg
     1801 ttattggtcc acatcaccgt ttcgctaact ggtacgttta acgatccttc cagaccttca
     1861 accactgctt tcggcacgtt atcccactga tagagatcag tgccgactgc gttttgccct
     1921 acagccacat agcccgcttc aatcttattg cgataatcgt tacggaacca ggtgacgccc
     1981 gccagccagc cgtcgcgttt gaactccaga ccaatctctt tgttgatgct ggtttctact
     2041 ttcaggtcat cgttaccttg cagatagcag ccgcccgcgc tggcatagca gccctgacct
     2101 ttactgtaga gaatgtagtt cgggttagtc tggtataggc tcggcgcttt ataggcgcgg
     2161 gcaatgccta ttttcagcgt gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg
     2221 ctccagttat tgccgacaat actgtgatga tcgaaacgca gccccggcgt tacgatggtg
     2281 ctgtcagtca gctccatgtt gttttcggca aacagcgaga aaatttctgc ttttgaatac
     2341 gggctacggt cggtggcact cacgccatca atagcgccac cggtattcgt tccggtcagt
     2401 gcttgggtgt tggaacttaa gtccttcatc cgttgctgat tccactccgt acccagcgtc
     2461 agcgtctggt taacgaggaa atcaatcggc aggttaactt cgctgtgcag catcacgtca
     2521 tcgagatcga tatcgacgaa atcctgtgtc gctttttcgt taaatttccc ttcggtaccg
     2581 cccgccagac cttccggaat acgcgagtta cgggtgtgtt catactgcac ccagttgctg
     2641 gtggtcacgc cgttatccca gccaccgttc caggtcagcg cgtagttctg gcgatacagg
     2701 cggttggttt catcgccata tttcgagcga gtgtaagcgt cagagttggt gttctgggta
     2761 tcgcccgcat acaggttacc ctggcggctg taacccgctt ccagttccag cgattgcaat
     2821 ggtgcgaagt cccagcgcac cacgccgtta atatctttat tgatcacccc ttcgcgcccg
     2881 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
     2941 cacgcgtcag cctgagtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
     3001 agcggaccgg tcaggctaaa gttagtgcgt ttggtggcac cttcctcttt atgttctggc
     3061 gcattgaaat atgcgtccca ggagccgtgc cactcgccgc tgcctttttt ggtaatgatg
     3121 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgcagaact
     3181 tcaatacgtt caatcatttc aggtggcacc caggaggtat caccacgggt atcgcgctcg
     3241 ccacgccagc cctgacgcac cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc
     3301 agcgtgtttt ccggacccat accgcgaata tcaatctgtc ggttattccc acgctgacca
     3361 ctggtggagt taccggtcag gttaacgcct ggcatggtac ggatgatctc cgacacatcg
     3421 cgggcaaccg ggtttttgcg gatttcatct gcggtgatgg tcgaaacgcc aggagcctgc
     3481 aagttctgct cggcggcggt aacgacaatg gtatcgtcat gtgaaacagg tgtatcggtc
     3541 ggctcttgtg cctgcgctac cccataaatc cccagattga ccaacaaggc cagggaatga
     3601 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
     3661 gtcgtcatca acacgacgca tcccgttacc gcgaaaacct ttgatcctga aagacacgca
     3721 gtgcagttgg ttaattaatg tccgcgcttc ccacggcgcg ccattacgct attgcaaatg
     3781 caaatagtta ttaataacat tatcaatata tttctgcaat caatgaaaaa ttgcacagta
     3841 aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa
     3901 acatggcccg gaatggcagc gtctgaatga cgaaatgttt gaggtcactt tctggtggcg
     3961 tgatccccaa ggttctgaag aatactcgac gataaagcgc gtatgggtct acatcactgg
     4021 tgtgaccgat catcatcaga acagccagcc ccagtcgatg cagcgaattg caggtactga
     4081 tgtctggcag tggacgacac aactcaatgc caactggcgc ggcagctact gctttattcc
     4141 caccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat tacgtgaagg
     4201 ctggcgaaaa ctattacccc aggcaatagc cgatccgctg aatccacaaa gctggaaagg
     4261 cgggctaggg cacgctgttt ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg
     4321 ggattgtccg caagcgccag aaataccagc caaagaaatt atctggaaaa gtgaacggtt
     4381 gaaaaattca cgtcgtgtat ggatttttac caccggcgat gtaacagcag aagaacgccc
     4441 gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct
     4501 gacttcgctg acccatcgtc agcaacttcc tcccgccgtg tatgtgttga tcgacgctat
     4561 cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc tggcagtaca
     4621 gcaagagtta ttacccctgg tgaaagttat tgcccctttt agcgatcgcg ccgatcgcac
     4681 cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg tatgccggac tgcactggcc
     4741 tgaacgcttt ggctgtgtat taagccagtc aggatcgtac tggtggccgc atcggggcgg
     4801 gcagcaagag ggcgtgttac ttgaaaagct aaaagctggt gaagttagcg ccgaaggtct
     4861 gcgcattgtg ctggaagcgg gtattcgcga gccgatgatc atgcgggcca atcaggcgct
     4921 gtatgcgcaa ttacacccta taaaagaatc cattttctgg cgtcaggttg acggcggaca
     4981 tgatgcgctt tgttggcgcg gtggcttgat gcaggggtta atcgacctct ggcaaccact
     5041 tttccatgac aggagttgaa tatggcattc agtaatccct tcgatgatcc acagggagcg
     5101 ttttacattt tgcgcaatgc gcaggggcaa ttcagtctgt ggccgcaaca atgcgcctta
     5161 ccggcaggct gggacattgt gtgtcagccg cagtcacaag agtcctgcca gcagtggctt
     5221 gaagcccact ggcgtactct gacaccggcg aatttcaccc agttgcagga ggcacaatga
     5281 gccagcattt acctttggtc gccgcacagc ccggcatctg gatggcagaa aaactgtcgg
     5341 atttaccctc cgcctggagc gtggcgcatt acgttgagtt aaccggagag gttgatgcgc
     5401 cattactggc ccgcgcggtg gttgccggat tagcgcaagc agatactttg cggatgcgtt
     5461 ttacggaaga taacggcgaa gtctggcaat gggtcgatga tgcgctgata ttcgaactgc
     5521 cagaaattat cgacctgcga accaatattg atccgcacgg tactgcgcag gcattaatgc
     5581 tggcggattt gcaacaagat ttgcgcgtcg atagcggtaa accactggtc tttcatcaac
     5641 tgatacaggt ggcagataac cgctggtact ggtatcagcg ttatcaccat ttactggtcg
     5701 atggcttcag ttttccggcc attacccgcc agatcgccaa catttactgc gcattgctgc
     5761 gtggcgaacc aacgcctgct tcgccgttta cgcctttcgc tgatgtagtg gaagagtacc
     5821 agcaataccg cgaaagcgaa gcctggcagc gtgatgcggc attctgggtg gaacagcgtc
     5881 gccaactgcc accgcccgcg tcactttctc cggcaccttt agcggggcgc agcgcctcgg
     5941 cagatatttt gcgtctgaaa atggaattta ccgacgggga attccgccag ctggctacgc
     6001 aactttcagg tgtgcagcgt accgatttag cccttgcgct gacggctttg tggctggggc
     6061 gattgtgcaa tcgcatggac tacgccgccg gatttatctt tatgcgtcga ctgggctcgg
     6121 cggcgctgac ggctaccgga cccgtgctca acgttttgcc gctgggtatt catattgcgg
     6181 cgcaagaaac gctgccggaa ctggcaaccc gactggcaac ccgactggca gcacaactga
     6241 aaaaaatgcg tcgtcatcaa cgttacgatg ccgaacaaat tgtccgtgac agcgggcgag
     6301 cggcaggtga tgaaccgctg tttggtccgg tactcaatat taaggtattt gattaccaac
     6361 tggatattcc tggtgttcag gcgcaaaccc ataccctggc aaccggtccg gttaatgacc
     6421 ttgaactggc cctgttcccg gatgaacacg gtgatttgag tattgagatc ctcgccaata
     6481 aacagcgtta cgatgagcca acgttaatcc agcatgctga acgcctgaaa atgctgattg
     6541 cccagttcgc cgcggatccg gcgctgttgt gcggcgatgt cgatattatg ctgccaggtg
     6601 agtatgcgca gctggcgcag atcaacgcca ctcaggttga gattccagaa accacgctga
     6661 gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc tccggcgctg gcagatgcgc
     6721 gttacctgtt cagctatcgg gaaatgcacg agcaggtggt ggcgctggcg aatctgctgc
     6781 gtgagcgcgg cgttaaacca ggggacagcg tggcggtggc actaccgcgc tcggtctttt
     6841 tgaccctggc actccatgca atagttgaag ctggcgcggc ctggctaccg ctggataccg
     6901 gctatccgga cgatcgcctg aaaatgatgc tggaagatgc gcgtccgtcg ctgttaatta
     6961 ccaccgacga tcaactgccg cgctttagcg atgttcccaa tttaaccaac ctttgctata
     7021 acgccccgct taccccccag ggcagtgcgc cgctgcaact ttcacaaccg caccacacgg
     7081 cttatatcat ctttacctct ggctccaccg gcaggccgaa aggggtaatg gtcgggcaga
     7141 cggctatcgt caaccgcctg ttgtggattc aaaatcatta tccgctcaca ggcgaagatg
     7201 tcgttgccca aaaaacgccg tgcagttttg atgtctcggt gtgggagttt ttctggccgt
     7261 ttattgccgg ggctaaactg gtgatggctg aaccggaagc gcaccgcgac ccgctcgcta
     7321 tgcagcaatt ctttgccgaa tatggcgtaa cgaccacgca ctttgtgccg tcgatgctgg
     7381 cagcatttgt tgcctcgctg actccgcaaa ccgctcgcca gagttgcgtg acgttgaaac
     7441 aggttttctg tagtggtgag gccttaccgg ctgatttatg ccgcgaatgg caacagttaa
     7501 ccggcgcgcc gttgcataac ctgtatggcc cgacggaagc ggcggtagat gtcagctggt
     7561 atccggcttt tggcgaggaa ctggcacagg tgcgcggcag cagtgtgccg attggttatc
     7621 cggtatggaa tacgggtctg cgtattcttg atgcgatgat gcatccggtg ccgccgggtg
     7681 tggcgggtga tctctatctc accggtattc aactggcgca ggggtatctt ggacgacccg
     7741 atctgaccgc cagccgcttt attgccgatc cttttgctcc tggtgaacgg atgtatcgta
     7801 ccggagacgt tgcccgctgg ctggataacg gcgcggtgga gtacctcggg cgcagtgacg
     7861 atcagctaaa aattcgcggg cagcgtatcg aactgggcga aatcgatcgc gtgatgcagg
     7921 cgctgccgga tgtcaaacaa gccgttaccc acgcctgtgt gattaaccag gcggcagcca
     7981 ctggtggtga tgcgcgtcag ttggtgggct atctggtatc gcaatcgggc ctgccgttgg
     8041 ataccagcgc attgcaggcg cagcttcgcg aaacattgcc gccgcatatg gtgccagtcg
     8101 ttctgctgca actgccgcag ttgccactta gcgccaacgg caagctggat cgcaaagcct
     8161 taccgttgcc tgaactgaag gcacaagcgc cagggcgtgc gccgaaagcg ggcagtgaaa
     8221 cgattatcgc cgcggcattc gcgtcgttgc tgggttgtga cgtgcaggat gccgatgctg
     8281 atttctttgc gctgggcggt cattcgctac tggcaatgaa actggcagcg cagttaagtc
     8341 gacagtttgc tcgtcaggtg acgccggggc aggtgatggt cgcgtcaacc gtcgccaaac
     8401 tggcaacgat tattgatggt gaagaggaca gctcccggcg catgggattc gaaaccattc
     8461 tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt ccatccggcg tccggttttg
     8521 cctggcagtt tagcgtgctc tcgcgttatc tcgatccaca atggtcgatt attggcattc
     8581 agtcgccgcg ccctcatgga cccatgcaga cagcgacaaa cctggatgaa gtctgcgaag
     8641 cgcatctggc aacgctactt gagcaacaac cgcacggcat tgcggcgcgg ctgcgtgccc
     8701 gtggcgaaca ggtggcattt cttggtttgc tggatacctg gccgccagag acgcaaaact
     8761 ggcaggaaaa agaggctaat ggtctggacc cggaagtgct ggcggagatt aaccgcgaac
     8821 gcgaggcctt cctggcggca cagcagggaa gtacttcaac ggagttgttt accaccattg
     8881 aaggcaacta cgctgatgct gtgcgcctgc tgacgactgc tcatagcgta ccgtttgatg
     8941 gtaaagcgac gctgtttgtt gctggacgca cgcttcagga aggtatgagc cccgaacgcg
     9001 cctggtcgcc gtggattgcg gagctggata tctatcgtca ggattgtgcg catgtggata
     9061 ttatctctcc aggggcgttt gtaaaaattg ggccgattat tcgcgcaacg ctaaacaggt
     9121 aaattaatat tatc
//
LOCUS       DAJTNP010000141         8990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTNP010000141 DAJTNP010000000
VERSION     DAJTNP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(140..2683)
                     /gene="mdoH"
                     /locus_tag="OO160_003666"
     CDS             complement(140..2683)
                     /gene="mdoH"
                     /locus_tag="OO160_003666"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5525633.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2676..4211)
                     /gene="mdoG"
                     /locus_tag="OO160_003667"
     CDS             complement(2676..4211)
                     /gene="mdoG"
                     /locus_tag="OO160_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5525634.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4605..5762
                     /gene="mdoC"
                     /locus_tag="OO160_003668"
     CDS             4605..5762
                     /gene="mdoC"
                     /locus_tag="OO160_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5525635.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5770..7251)
                     /gene="clsC"
                     /locus_tag="OO160_003669"
     CDS             complement(5770..7251)
                     /gene="clsC"
                     /locus_tag="OO160_003669"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5525636.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7193..7726)
                     /gene="ymdB"
                     /locus_tag="OO160_003670"
     CDS             complement(7193..7726)
                     /gene="ymdB"
                     /locus_tag="OO160_003670"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5525637.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7821..8132)
                     /locus_tag="OO160_003671"
     CDS             complement(7821..8132)
                     /locus_tag="OO160_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5525638.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8253..8585)
                     /gene="csgC"
                     /locus_tag="OO160_003672"
     CDS             complement(8253..8585)
                     /gene="csgC"
                     /locus_tag="OO160_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5525639.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8644..>8990)
                     /gene="csgA"
                     /locus_tag="OO160_003673"
     CDS             complement(8644..>8990)
                     /gene="csgA"
                     /locus_tag="OO160_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5525640.1"
                     /translation="NSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADV
                     GQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQ
                     VGFGNNATAHQY"
BASE COUNT         2248 a         2328 c         2133 g         2281 t
ORIGIN      
        1 acgccggtat ttttattggc tcgttgtttt tgccggatgt ggcgtgaacg ccttatccga
       61 cctacaaaat cttgcaaaat caataaattg caggaacgat gtaggcctga taagcgtagc
      121 gcatcaggca actacgtttt tattgcgaag ccgcatccgg tttacgcaat gccagtggat
      181 tgagctttat cccttcgtaa taactcaccc atgaagaata tctttcaggg gaattccaga
      241 cgcggaaatg cagacgggcc atcgtcaccg gatcgcttag cagcaccagg cgacgatcgc
      301 gattcagctt ctctggcgtc tcgttaagcg cctgttcaac gtggcggtca cgggcgattt
      361 ccagcacctt actggcgcgg tgacgcgctg tcgccattgc ggttgccaga gcgttaaatg
      421 acgggttaaa cactgcgtgc ataaagccat cgtcaaggga gcgttggcga ttcatctcaa
      481 ggaaccgatc ggtatcaacc agcacctgcg gcggtgaata ctcttccggg atcaggaaca
      541 gtttccagcg tttggtgcgc agaccaacgg tggcacggct ggaaatcacc gaaacaaacg
      601 gtgacaggat caacgagaag acaatcggtg ccagccagaa caggaaacgc agatccagcc
      661 acgccatccc aacagcccac actaacccca acagcagctg tgagccgtgg cgtttgaacg
      721 cttcgcccca tgaagtggag tcatcatcac gctgcggtga attccacacc acttcccagc
      781 caaggaacgc gctgacaacg aagaccgtat ggaacagcat gcgtaccgga gccagcagca
      841 cggaaaacag cacttccagc agcaacgata atgtaacgcg ccagaagccg ccgtattctt
      901 tcgttccttt gcaccagata agcaaaatgc tcaataactt cggcaggaac aacagcacca
      961 tggtcgaagc aaaaagtgca atcgccagct caggacgcca ctgcggccac accgggaaca
     1021 actgccgtgg ttgcaggaag tattgcggtt cggtcaacgc atgcactacc tgcaatgcag
     1081 tagagagcgc gaggaacata aaccacagcg gagcggagag ataagacatc acgcccgtca
     1141 ggaacaccgc acggtgaacc gggtgcatac ccttaaccag gaacagacgg aagttcatca
     1201 ggttaccgtg gcaccagcgg cggtcacgtt ttagctcatc aagcaagtta ggcggcaatt
     1261 cttcataaga acccgggaga tcgtaagcaa tccagacccc ccaacctgca cggcgcatca
     1321 acgccgcttc tacgaagtca tgtgacagga ttgaaccggc aaaggaacct tcgcccggca
     1381 gcggagccag tgcgcagtgc tcgataaatg gtttcacgcg gataatcgcg ttatgtcccc
     1441 agtagtgcga ctcgccaagt tgccagaagt gcaaaccggc tgtaaacagt ggcccataca
     1501 cgcgggtcgc gaactgctga cagcgcgcat acagcgtatc cataccggac gctttcggcg
     1561 acgactgaat gatcccggcg ttcgggttgg cttccatcaa gcgcaccagg ccgcataaac
     1621 aatcaccggt cattaccgag tcagcatcca gcaccaccat gtagctgtac tggctgcccc
     1681 agcgacggca gaagtcatcg atattaccgc ttttacgctt cacgcggcga cggcggcggc
     1741 gatagaaaat ctgaccttcg ccaccgactt cagcgataag ctccatccag gctttttgct
     1801 ctgcgacgca gatatccggg ttataactgt cactcagaat gtagacatca aagtgtttgg
     1861 cattcccggt ggcttttact gattcccacg ttgcacgcaa gccagcaaaa acacggttca
     1921 cgtcttcgtt acagataggc atgatcaacg ccgtgcgatg ctccgggttt aatggttcat
     1981 cgccaactgt tgacgcagat atactgtatt tatcgcgacc aataagcagt tgcaggaagc
     2041 ccatcaacgc cgtccagaat ccggcggaca cccaacagaa cagtaccgca aaaaggatca
     2101 ggataccggt ttgcagcata taaggcagaa gctgcataaa ggaaacccac aaatcctgac
     2161 caaccatatc cataggatta atcagcgccc aaccctgata aggaagaatg gtcttcatat
     2221 accaggtcgc gacaacagtt tgcgcgagcg tcaggatcaa cagaatgtaa cggcggatgg
     2281 taccaacggt acgccacttt tgctcgctct cctgctcttc tttggtcaaa cgagccagat
     2341 agcgcggcgt gacatcgcgt ccacgcaggc gatcccagaa acggcctacc gggttggtac
     2401 gccacgggtc gggaaacatc gaggagcgtt ttgcttctgg catcgcctta agctgatcgc
     2461 gcccttcgtc gtctttaatt aactgtccat cagcaagtga atctggccag gcttgttcca
     2521 gacgcgcctt taccgagcct tgcggggaat catcctcccg cgcccaggtg cggtgttcgg
     2581 catccagcgc ctgatgaacg gcgcggatat cagtcttcgg caatgctgct ttctcgcttg
     2641 cggcgatggg cattgcgtca atgtactcag ttgtcttatt cattggcagg taactggtag
     2701 ctccaggttt cactcaacgt ctgatcggca ttcaccagcg cagcacgcat ttcagtgatt
     2761 ttcttggcat ctttcacttt cacacgcatc accagacgcc agcctttggt aaccgggtta
     2821 tagcgcaccg tgctttcaac tatctcacca ttatcaccaa tgctggtttg cgctgtgacc
     2881 ggggtatcct ctggcagttt tttcatctct gcgccggtaa aatcgaccac aaaggcgata
     2941 gtaccgtcag gctggcgaat caggttcgac tgcttcacat cccccgttga acgacgcgtt
     3001 tgttgcaccc atgcgttatc tggcgcatgc agtttgtctt catcacggct gaaggtgatg
     3061 gtgtatttaa agttcatctc tttacccggc tccggcagct gatccggcgt ccagtaagcg
     3121 acgatgttat cgttggtttc atcgttggtt ggaatttcca ccagctcaac gctgcctttg
     3181 ccccactccc ctttcggagt cacccatgcg cttggacgga gatcgtaacg atcatcgaga
     3241 tcttcaaagc gggagaaatc acgacctcgt tgcaacagac caaagcctcg cgggttttcc
     3301 atggagaagc tgctgaccgc taaatgtttc gggttattca acggacgcca gatccattcg
     3361 ccattaccag catggataga cagaccgtta gagtcgtgca actccggacg atagttattt
     3421 gcaggcgacg gttggttcgg cccaaacagg aacatactgg ttaacggtgc aacccccagt
     3481 ttgccgactt tatcgcgcag atagattttc gactgcacat ccacaaccgt gtcacgccct
     3541 ggcataacca cgaatttgta agcacctgtc gcacgcggcg agtcaagcaa tgcataaatg
     3601 gttaaacgtt tatcagtcgg ttttggacgc tcgatccaga actctttgaa gcgtggaaat
     3661 tcttcacccg atggcaaagc ggtatcaatt gctaggccgc gggcagaaag gccataaacc
     3721 tgacctgcac caatcacgcg gaaatagctg gccccgagca tgctgacgat ttcatcgttt
     3781 ttatctttgc tgttgatcgg gtaaagcact ttaaaaccgg caaaaccaag gtcttttacc
     3841 gtgtctttgt catgctgaac atcgccgaaa gtgaaataat ccgggctgta tttgattcgt
     3901 ttgactgcgg tggcagtcac ttcatttatt ttgaccgggg tatcgaagta cataccctga
     3961 tggtagaact cgagtttgaa tggggtcttc agattgttcc agtacgcttt gtcatgatta
     4021 aactggatct gctgatagtc cgcgtatttc atgtcgcgga aaacggaggg caagttgctt
     4081 ttaggcgcct catagccttt cccggctaag gattgagctt gctttgcgac atcatcaata
     4141 ctgaaagccc agcttgaaga tgtatacagg gttaacatta ctgcagcact caaccaacgc
     4201 attttcatca tttgtagttt atgtttcata attagtaagc acttccccct ttgtgtgctt
     4261 atatcgatcc gatctatttt aatggataac cagccattcc gacaactgaa tcccggtatc
     4321 gttcagcacg ctggctcatg gattaagcac ctgattgcaa ccgttttcac tttccgtgct
     4381 tctcctaaag acagaagcat cgacttcgtg tagggtttgc cagcaaattt atttatcaac
     4441 cgtcttacag acgcttccat tctggttagt ggtagcgatg atgctatgca taaaaacctt
     4501 gaagcctgcg cctggcgcgg tcaggtcggt gtcgctatgg caacaagcct aaaggcataa
     4561 cccgacaggg ataagacgaa aaatccgaga ttagttaacc atatatgaat ccagtacccg
     4621 cgcaacgtga atatttcctc gactccatcc gcgcctggct gatgttgtta gggatccctt
     4681 ttcatatttc tttaatctat tcgagccata catggcatgt gaatagcgcc gaaccgtcat
     4741 tgtggctaac cctttttaat gacttcatcc actcgttccg catgcaggta tttttcgtta
     4801 tatccggata cttttcctac atgctttttt tacgctatcc cttgaaaaaa tggtggaaag
     4861 tacgtgtcga acgtgtaggt atcccgatgt taacagccat ccccctactg acattaccgc
     4921 aatttattat gctgcaatat gtcaaaggga aagcggaaag ttggcctggg ctatcattgt
     4981 atgacaaata taatacgttg gcctgggaat taatatcaca cctgtggttt ttactggtgt
     5041 tagtagtcat gacgacgctg tgtgtatgga tatttaagcg catcagaaat aatttagaaa
     5101 attctgataa aacgaataaa aaattctcga tggtaaaact atcggtgatt tttttatgcc
     5161 tcggcatcgg ctatgcggta ataagaagaa cgatttttat tgtgtatcca cccattctga
     5221 gtaatggcat gttcaatttt attgtcatgc aaacgctatt ttatttaccg ttctttatcc
     5281 tcggcgcact ggctttcatt ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg
     5341 gctgtaccct cgctgcagca ttggcgtttg tcgcttattt actcaaccag cgctatggca
     5401 gtggcgatgc ctggatgtac gaaaccgagt cggtgatcac catggtcctc ggcctgtgga
     5461 tggtgaatgt ggtcttctcc tttggccacc gtttgcttaa cttccagtca gcgcgggtga
     5521 cttattttgt taacgcatcg ctgtttatct atctggttca ccacccgtta acgctgtttt
     5581 tcggcgcata cattacaccg cacatcacct ccaactggct tggttttctc tgtggcctga
     5641 tattcgtagt agggattgcg ataattctgt atgaaattca tttacgcatc ccgttactga
     5701 agtttttgtt ctctggtaaa ccggttgtta agcgtgagaa cgataaagca ccagcccgtt
     5761 aagccacatt tacaataacc attccacggg caatatcgac gccagtctga ccataacccg
     5821 cttccagaaa ctggtggcgg gttctttttt gagaataacc tctttctttg catgacgatc
     5881 aacccagttg atccgtcccc acctgtccag acggagctgc caggccgcat cgtactggct
     5941 ctgaataaag cgtttatcaa ttaactgtgc cagcgtttcg ctctctatca cgaagcccat
     6001 ttcagtattg agcaatgttg aacgcggatc gaaattgaaa gagccgataa acaccgtttt
     6061 accatcgatg ctaaaagttt tagcatgcag gctggcaccg gaattaccgg ttatgccgcg
     6121 atcgtgtaac gtactgcttt gttcacgcgt cggcttgagt tcatataatt ccacgccata
     6181 gcggagcaat tttttgcgcc agcgcgcgta tccggcatgg acgacagcaa catcgttagc
     6241 ggcaagagaa ttggttagga tcgcaatctt tacccctttt ctcaccatcc gtaagagttg
     6301 cgccacacct gcgcgtgtcg gtacaaaata ggaagagata atatcgatgc gttcactggg
     6361 tgagcccatg atatcgaaca ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc
     6421 ccctttcgcc ggatcatcac ttaataaacg tgtcttcgcc cagataagcg gcaacgttcc
     6481 atcaaccaga tgatttataa atggactgga ttccattttg cgtaaataac gatgcgtcat
     6541 ggcatcgtta tgccaggagg cgggtaactc gatgcgatcc gccatttcac cttccgggac
     6601 atccagcacc tgctgtaagg gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc
     6661 ggcaacatcc tctaccacgg ggcctattgc catgacatct aaatccgaaa aaagtggctc
     6721 ttcccctgca ccaaaatagg catcaccaat atttcgtcct cccactaggg taaccacgcc
     6781 atcgacagtg aaacttttat tgtgcatccg gcgattgaga cgggaaaagt cggtgatata
     6841 tccaagcgga cgtagcaagc gaaacgagaa aggattaaaa agccggactt caatgcgtgg
     6901 atgactgtca agcaagcgca aaatgtcgtc aagtccgggc gtattgttgt cgtccagcaa
     6961 caaacggacg cgaacgccac gctttgctgc ggctaacagg gcggaaaaca gtaaccgccc
     7021 cgacatatcg tcctgccaga tgtaatactg aacatccagc gtatgctctg ccatttcggc
     7081 cagacgatac cgggcggcaa acgcatccag acttttctcc agagggaaaa gaccacactg
     7141 accgggatgt tgcgaacaca gtggcagcac cgcgctcgcc agccggggca aatcattcat
     7201 ctccttgttg ggtaaggagt ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa
     7261 agtatacctg ttcgggtaaa gcgtgacggg taataaattc tgaaacggtt ttaactgcga
     7321 tttcagccgc tgccgcacga gggtaactat aaaccccagt actgattgca ggaaaagcca
     7381 ctgacgtata gctgtttgcc gccaccagtc gtaggctatt cagataggca tcctgcaaaa
     7441 gctggtcttc gttttgttcc ccaccacgcc agactggccc gacggtgtgt actacggctt
     7501 tagcgggaag atcgcctgca agcgtaataa cggcatgccc cgtagggcaa tcgccctgct
     7561 gttgcctgac ttttaaacag gcatccagca gggccggacc cgctgcgcga tgaatggccc
     7621 catcgacgcc gccgcctccc attaatgacg gattagccgc attcacaatc acatcaacgg
     7681 ccagtttggt aatatcaccc tgcacaacat gaatacgcgt tttcataacg cctccttaca
     7741 ccttcttgtt tcttaagtgt acagcaggct ttttggttaa atcgcatttt tctgctcagc
     7801 aacgtgagtt gttcagaaac ttagttatat tcgatgttca ttacagccag ctttttctgt
     7861 ttatcaagat actgcacttt tactgaggct atttgcgcgt ttttgacatt gccgctcatt
     7921 aacgctttgc tggagtaggt tttgcccgga atagaaccat tctgtgggca gttcatttct
     7981 acgggtgatg actgggtgct gacgtcacat gccggttcca caatttggcc ataaaaatgg
     8041 atcacgcccc cttgcgcggt gcttcctgca atggcaataa aaggaaaaaa caaactgccg
     8101 agcataagag agtctaaaaa aggtctgaac atttattcta tttcctcaat gattagtcat
     8161 ccttgaaggt tgtgttatcc atactttcag gttggctgat tcgcagcaga ccattctctc
     8221 cagattcatc ttatgctcga tatttcaaca aattaagact tttctgaaga gggcgtccat
     8281 tgttgtgata aatgaagtgc ctgtccatca gaaacagtaa caactatttt cacccgatct
     8341 tccggggaaa tatttaaact caacttcgtc aaagcaatgg gttgattagc aggcaatgag
     8401 agggtctttt cttgcttcgt ctgactttgc cctgaactgc cttcgcgcag ggacaatatt
     8461 tgtactctgc acagacaaga ttgagtaaga gtaacttcag gaataatggt gtacatatcc
     8521 ccttgctggg tcgtattaaa ggttatctgg ctggaaagtg ccgcaaggag taataacgca
     8581 ttcatattct tctcccgaaa aaaaacaggg cttgcgccct gtttctgtaa tacaaatgat
     8641 gtattaatac tgatgagcgg tcgcgttgtt accaaagcca acctgagtca cgttgacgga
     8701 ggagttcgat gcagtctggt caactgcagc accgttgcca ccaccgaact gtttaacagt
     8761 catttcagaa tttttgccgt tccactgatc aagagtggcg ctgttaccga agccacgttg
     8821 ggtcagatcg attgagctgt catctgagcc ctgaccaaca tctgcaccat taccaccgcc
     8881 atgctgggta atagtcaagt cagagttacg ggcatcagtt tgcagagcaa gtgcagagtt
     8941 accgccaccg tactggtaaa tgttcagctc tgaatttggg ccgctattat
//
LOCUS       DAJTNP010000142         8771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTNP010000142 DAJTNP010000000
VERSION     DAJTNP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO160_003674"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO160_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5525641.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="OO160_003675"
     CDS             complement(984..1814)
                     /locus_tag="OO160_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5525642.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="OO160_003676"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="OO160_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5525643.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="OO160_003677"
     CDS             3240..3590
                     /locus_tag="OO160_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5525644.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="OO160_003678"
     CDS             3716..4276
                     /locus_tag="OO160_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5525645.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="OO160_003679"
     CDS             4279..7245
                     /locus_tag="OO160_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5525646.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="OO160_003680"
     CDS             7312..7689
                     /locus_tag="OO160_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5525647.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="OO160_003681"
     CDS             complement(7890..8549)
                     /locus_tag="OO160_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5525648.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2480 c         2348 g         1993 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DAJTNP010000143         8679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTNP010000143 DAJTNP010000000
VERSION     DAJTNP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO160_003682"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO160_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5525649.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3216)
                     /gene="hemX"
                     /locus_tag="OO160_003683"
     CDS             complement(1969..3216)
                     /gene="hemX"
                     /locus_tag="OO160_003683"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5525650.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(3238..3978)
                     /gene="hemD"
                     /locus_tag="OO160_003684"
     CDS             complement(3238..3978)
                     /gene="hemD"
                     /locus_tag="OO160_003684"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5525651.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWYFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3975..4916)
                     /gene="hemC"
                     /locus_tag="OO160_003685"
     CDS             complement(3975..4916)
                     /gene="hemC"
                     /locus_tag="OO160_003685"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5525652.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5303..7849
                     /gene="cyaA"
                     /locus_tag="OO160_003686"
     CDS             5303..7849
                     /gene="cyaA"
                     /locus_tag="OO160_003686"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5525653.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7889..8209)
                     /gene="cyaY"
                     /locus_tag="OO160_003687"
     CDS             complement(7889..8209)
                     /gene="cyaY"
                     /locus_tag="OO160_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5525654.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8257..>8679)
                     /locus_tag="OO160_003688"
     CDS             complement(8257..>8679)
                     /locus_tag="OO160_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5525655.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1974 a         2241 c         2241 g         2223 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtt tagcttccgt
     2101 tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca gtttttccag
     2161 catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct ggcttaactg
     2221 gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt aagcacgtac
     2281 ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac gcggtacagc
     2341 ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat cctgatttgg
     2401 cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt tgtccataaa
     2461 gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg aaagctcttc
     2521 accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca ggttatctac
     2581 ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct gcgatactgc
     2641 agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac tcgggtcatt
     2701 catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagaggtcg tgacgtcctg
     2761 atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag ccagcagcca
     2821 ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat ccaactgttt
     2881 tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt gcttaataat
     2941 gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca gttggttagc
     3001 cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc catacaaacc
     3061 gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg cagtattgtt
     3121 cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct ccctggtctc
     3181 ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt atgagagtta
     3241 ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa tgtcttgcca
     3301 gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc gacagtgtag
     3361 taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca acatttcacc
     3421 gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt cttctgcacc
     3481 atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag caccgcgcac
     3541 cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca gcgcacgttt
     3601 gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt cccgatcctg
     3661 tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg tgcgtccaat
     3721 ggcgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct gtgattgggc
     3781 aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca gcgctgccag
     3841 ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg gaaaatacca
     3901 ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg gagacgggcg
     3961 ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag gatctcgcgc
     4021 gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc atcttgcggc
     4081 gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac cagcgcacat
     4141 agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg acatccgcct
     4201 tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc gtggtgattc
     4261 agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat accgactgca
     4321 ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat gcgggactcc
     4381 agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc gccgttatct
     4441 agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag atccgggcgg
     4501 cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact gcctgctggt
     4561 aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc ttcacgctcg
     4621 caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt cattgaatgt
     4681 acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac aaataagcct
     4741 tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt caccatcggt
     4801 accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac atagtgtgcc
     4861 tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc taacatgctt
     4921 gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga tgtcagtgtt
     4981 gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg gatcctgaca
     5041 ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta cacttgtatg
     5101 tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt tttagaccat
     5161 tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta cctttgacat
     5221 acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca gtcatgacgg
     5281 gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac agagactgga
     5341 tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg cattccaaca
     5401 ggtctacagt ctactgccga cattgttgca ctatcaccat ccgcgaatgc cgggttacct
     5461 tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc aacgccacta
     5521 cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga aaggtgagct
     5581 tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa gttgttcctc
     5641 tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc gccagttact
     5701 acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg aagtcagctt
     5761 cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg ggggcgaaga
     5821 ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg ccgtgcgtct
     5881 cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc attacgacga
     5941 ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc tggatctcgg
     6001 tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc agctctacaa
     6061 gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag cctattcctg
     6121 ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc acgacggcga
     6181 gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta ctgaatacct
     6241 gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct atttaaaagt
     6301 gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag tgttgagcca
     6361 gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata accgtgctaa
     6421 ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga tgatgcagag
     6481 ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg ccagtccgca
     6541 ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc caggtaaagt
     6601 gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga cctttattta
     6661 tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg cgccaaatat
     6721 tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata aactggtggc
     6781 gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta aaggtaacgg
     6841 cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc atttcccgct
     6901 gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc gccatctggc
     6961 gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg tggtgcattt
     7021 tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc tggtaggtag
     7081 cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact tcaacggcga
     7141 gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg acgccgcacc
     7201 gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa tccgtactcg
     7261 cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc gccaggaaac
     7321 cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct tcgaacgcct
     7381 gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta tttcgcataa
     7441 caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac cggcggtggt
     7501 ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc aagacgagaa
     7561 tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc accactgcga
     7621 aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat cgcacgaccg
     7681 cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc agattgtgaa
     7741 ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca acctgccgcc
     7801 cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat gagcgtgccg
     7861 gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca cccgcctgtt
     7921 gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg caaatccatt
     7981 catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac acctggtgca
     8041 gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg gtcagtacgc
     8101 cgccgttgat ttcgcagtcg atatcgctgt cgccatccct gtcgtccaga cgctcttcaa
     8161 tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt gttgtatcct
     8221 gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag tttgcacctc
     8281 tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc tgttttgaac
     8341 ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg gcgtaatcct
     8401 tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc gatcgttttt
     8461 agttagcgaa ccgttaactg gaataatttg caagctcttt gaccgtaatt ggcggataaa
     8521 gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg gcgtatccag
     8581 cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat acagccgatc
     8641 tttatattgc gcaatattgc cagtaatcag atggaattg
//
LOCUS       DAJTNP010000144         8503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTNP010000144 DAJTNP010000000
VERSION     DAJTNP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..104)
                     /locus_tag="OO160_003689"
     CDS             complement(<1..104)
                     /locus_tag="OO160_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5525656.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
     gene            149..328
                     /locus_tag="OO160_003690"
                     /pseudo
     CDS             149..328
                     /locus_tag="OO160_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(337..735)
                     /gene="vapC"
                     /locus_tag="OO160_003691"
     CDS             complement(337..735)
                     /gene="vapC"
                     /locus_tag="OO160_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5525657.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(735..962)
                     /gene="vapB"
                     /locus_tag="OO160_003692"
     CDS             complement(735..962)
                     /gene="vapB"
                     /locus_tag="OO160_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5525658.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1044..1415
                     /locus_tag="OO160_003693"
     CDS             1044..1415
                     /locus_tag="OO160_003693"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5525659.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1436..6297
                     /gene="traI"
                     /locus_tag="OO160_003694"
                     /pseudo
     CDS             1436..6297
                     /gene="traI"
                     /locus_tag="OO160_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6317..7063
                     /gene="traX"
                     /locus_tag="OO160_003695"
     CDS             6317..7063
                     /gene="traX"
                     /locus_tag="OO160_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5525660.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7118..7678
                     /locus_tag="OO160_003696"
     CDS             7118..7678
                     /locus_tag="OO160_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5525661.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7809..8021
                     /locus_tag="OO160_003697"
     CDS             7809..8021
                     /locus_tag="OO160_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5525662.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8265..>8503
                     /locus_tag="OO160_003698"
     CDS             8265..>8503
                     /locus_tag="OO160_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5525663.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1954 a         2122 c         2707 g         1720 t
ORIGIN      
        1 aataatcggg aagcgtcttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgtcgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcaga tcagtgaatc
      181 cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc atccggacac
      241 ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg aacgcgggga
      301 ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt cagttctcag
      361 gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc cccgactgcg
      421 ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac gggcaagttc
      481 tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa catcaatgcg
      541 ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt tttctgcacc
      601 atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt tcaggttaaa
      661 acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt tggtatcgag
      721 cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc cctgttatcc
      781 ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc tccggctggc
      841 gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt ttccggcaat
      901 gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac ggtggtttcc
      961 attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat tgataccggg
     1021 atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg gccggaagtg
     1081 ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg ggagaacgct
     1141 gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag gatgtcttta
     1201 cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg caggatggca
     1261 gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt atctccatga
     1321 tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc gtggattttg
     1381 ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag aaacggtgct
     1441 gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc aggaaccaca
     1501 gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat ggaagacact
     1561 gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta atcagattgc
     1621 ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg gctatgaaac
     1681 tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg aggccttttc
     1741 cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga aatcccggga
     1801 tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag tcagaatggc
     1861 cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc gtgacgcagc
     1921 ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg acgggccgga
     1981 tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag tgcagtttat
     2041 gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg gtgtgattga
     2101 acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc ccctcgatcg
     2161 tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag tccgggcact
     2221 cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa gtgtcccccg
     2281 gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt cgctggccat
     2341 tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac tggtcatgat
     2401 ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct cgcagatgaa
     2461 cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc agttgctgga
     2521 aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa aactctccct
     2581 gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg tcctgataac
     2641 cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg atgccggggt
     2701 gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca gtgaaccgga
     2761 ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga agagagcgtg
     2821 gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg cagtgagctg
     2881 aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc accggtctgg
     2941 ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt gatggagcag
     3001 tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac ggcgcagagt
     3061 cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat ttcttccctg
     3121 gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg tgagcgtctg
     3181 agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct gcaggtggca
     3241 tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc ggccaccctg
     3301 cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt ggaaacgccc
     3361 gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat ggcaatggac
     3421 aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta ttcctcactg
     3481 gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt ggtttctgag
     3541 cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct gcagaaagcc
     3601 gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct ggaaagtaaa
     3661 aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc tgcagaagga
     3721 accggttttg ctgacctggg aggggaaatc aatgtgcaga taaaacgggg tgatttactg
     3781 tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc gtcgtatgag
     3841 gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac gccgctgatg
     3901 gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc cgccacccgt
     3961 atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg tgtgggtaag
     4021 accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag tgaacgtccc
     4081 cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag cgccggcgtg
     4141 gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg cagcggagaa
     4201 actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt gggcaatacc
     4261 gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt ggccagcggt
     4321 gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca gcagacgcgc
     4381 agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga actgcgggag
     4441 gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct tgagcgtgtg
     4501 aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc cgtgacggag
     4561 ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat gctgaaaggc
     4621 gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga ctataccggc
     4681 aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga ggaccggcgc
     4741 gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg gaaggtgcag
     4801 gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg tcgtctctcc
     4861 acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca ccggattgcc
     4921 ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa cacgcggctg
     4981 atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga caccatccgg
     5041 gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg ttatgtggcc
     5101 aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc tgacggtcag
     5161 cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga cctggcctat
     5221 gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc gcttgaaggc
     5281 acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc cctgtcgcgt
     5341 atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga tgccatcaac
     5401 aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga ccgggaggtc
     5461 atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc ggcaggccgt
     5521 gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt tattgctccg
     5581 ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa cggcaagtcc
     5641 gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg gggattcagc
     5701 ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca gggcagccgt
     5761 aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc ccgtgataat
     5821 cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa tcccggtgcc
     5881 atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc gggagccgga
     5941 aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga ggccatccgc
     6001 cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa caaacctgac
     6061 ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga gcgtgaccgg
     6121 gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga accacaacgg
     6181 gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg tctgcagcag
     6241 atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg agactgatac
     6301 aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg attcactggc
     6361 tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac agcgggatat
     6421 catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga tattccagct
     6481 gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg ccctggtgtg
     6541 ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc ggctgtgggg
     6601 atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt atgaggggaa
     6661 tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa cgcgcagtgg
     6721 gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt ccggcaccag
     6781 ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc gggcggaaga
     6841 cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg cgcttaacct
     6901 tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga ccgttggtct
     6961 ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt tcccgacgtt
     7021 ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg
     7081 gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga agcgaccggt
     7141 actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga aaaccatcat
     7201 caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg agaaagcggc
     7261 ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt ccatttatct
     7321 gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc cgggattatt
     7381 tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac tggaagacgt
     7441 ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga aggccatcac
     7501 ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg
     7561 gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc gtctggcgaa
     7621 aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg agaaataaaa
     7681 aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat aacttccgct
     7741 tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa ctaaaggggg
     7801 acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg cgttaccagc
     7861 gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa gcggcagaag
     7921 catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt gcccggaaaa
     7981 gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag cagccttcat
     8041 tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa tatccggtcg
     8101 ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa gtcgattttt
     8161 tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg cactattcag
     8221 gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg aaatatattc
     8281 cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat ggacgggttg
     8341 ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg gtgcggatca
     8401 ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg tcaacagaca
     8461 tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac ata
//
LOCUS       DAJTNP010000145         8435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTNP010000145 DAJTNP010000000
VERSION     DAJTNP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..305
                     /locus_tag="OO160_003699"
     CDS             <1..305
                     /locus_tag="OO160_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5525664.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2187
                     /locus_tag="OO160_003700"
     CDS             277..2187
                     /locus_tag="OO160_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5525665.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2188..2391
                     /locus_tag="OO160_003701"
     CDS             2188..2391
                     /locus_tag="OO160_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5525666.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OO160_003702"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OO160_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OO160_003703"
     CDS             3971..5476
                     /locus_tag="OO160_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5525667.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OO160_003704"
     CDS             5513..5860
                     /locus_tag="OO160_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5525668.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OO160_003705"
     CDS             5918..6946
                     /locus_tag="OO160_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5525669.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OO160_003706"
     CDS             6998..7384
                     /locus_tag="OO160_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5525670.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OO160_003707"
     CDS             7396..7773
                     /locus_tag="OO160_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5525671.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..8344
                     /locus_tag="OO160_003708"
     CDS             7760..8344
                     /locus_tag="OO160_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5525672.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8341..>8435
                     /locus_tag="OO160_003709"
     CDS             8341..>8435
                     /locus_tag="OO160_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5525673.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPA"
BASE COUNT         1960 a         1989 c         2599 g         1887 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccgatgtcc ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc
     8281 ggaaatgccg aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa
     8341 atgaaccgac acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga
     8401 gacagcgcca cgttttttga cgggcttccg gcatt
//
LOCUS       DAJTNP010000146         8391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTNP010000146 DAJTNP010000000
VERSION     DAJTNP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(213..2375)
                     /gene="uvrD"
                     /locus_tag="OO160_003710"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(213..2375)
                     /gene="uvrD"
                     /locus_tag="OO160_003710"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5525674.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2459..3175)
                     /gene="yigB"
                     /locus_tag="OO160_003711"
     CDS             complement(2459..3175)
                     /gene="yigB"
                     /locus_tag="OO160_003711"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5525675.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3175..4071)
                     /gene="xerC"
                     /locus_tag="OO160_003712"
     CDS             complement(3175..4071)
                     /gene="xerC"
                     /locus_tag="OO160_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5525676.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4068..4775)
                     /locus_tag="OO160_003713"
     CDS             complement(4068..4775)
                     /locus_tag="OO160_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5525677.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4772..5596)
                     /gene="dapF"
                     /locus_tag="OO160_003714"
     CDS             complement(4772..5596)
                     /gene="dapF"
                     /locus_tag="OO160_003714"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5525678.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5633..5836)
                     /locus_tag="OO160_003715"
     CDS             complement(5633..5836)
                     /locus_tag="OO160_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5525679.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6025..7503
                     /locus_tag="OO160_003716"
                     /pseudo
     CDS             6025..7503
                     /locus_tag="OO160_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7500..>8391
                     /locus_tag="OO160_003717"
     CDS             7500..>8391
                     /locus_tag="OO160_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5525680.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         1938 a         2321 c         2156 g         1976 t
ORIGIN      
        1 ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt cgagcggaaa
       61 ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg atgcggcgta
      121 aacgccttat ccggcctaca tggcgttgca atttattgaa tctgaatgat tttttaggcc
      181 aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg ggcgtaagct
      241 gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct atgttcaccg
      301 ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac acgctggccg
      361 agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg gctgaccggg
      421 cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa ctcgccaata
      481 aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc cgcgtaggtc
      541 agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg ttcttcttcc
      601 agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc cataccgacg
      661 ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag ttgtaccgca
      721 tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga taggaacgcc
      781 tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt tgccgtcacc
      841 agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg ctcgtacatg
      901 gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg catatcggca
      961 gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct ggcagcacgt
     1021 ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt taactggcga
     1081 tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg cgttggcgta
     1141 ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg cagatacgag
     1201 agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat acggtacggc
     1261 atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact gcggtagaga
     1321 atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg gttaaccaca
     1381 aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc accgtccgcg
     1441 ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag ggcgttagcg
     1501 gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat ttcggcaccg
     1561 gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca gccgtagatt
     1621 gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag caggcggatc
     1681 cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt ggtaaaacgt
     1741 tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc gcgcagcagc
     1801 agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc ctgatacacc
     1861 ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg acgcaggcct
     1921 tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg cttctcgtcg
     1981 aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct atcgaggatc
     2041 tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg gtgcgccagc
     2101 ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg tccgatacga
     2161 tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga gtatggcgag
     2221 cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt cttaccactg
     2281 cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc gcgttgtttg
     2341 tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta aaaataggtt
     2401 gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga tttgctgatt
     2461 atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt aaacggctgt
     2521 cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc attccgctgc
     2581 gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc tcgccgatcg
     2641 gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt gagcgcccgt
     2701 gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt tccggctggg
     2761 cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt aaggtgtcgt
     2821 gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc attgctgcgt
     2881 gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct tgttcaatcg
     2941 aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct
     3001 ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga taattttgca
     3061 caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg taaagggtat
     3121 catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa cgcattattt
     3181 cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag
     3241 atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct gcacaccacg
     3301 aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt tatgtgggtg
     3361 aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct gcacattacg
     3421 cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc taccaaacag
     3481 gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg
     3541 gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt cgaggtgttt
     3601 gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca cttccagcat
     3661 tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc gattcatatc
     3721 gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa ctcctttcgc
     3781 cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac gtagcgcaga
     3841 aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta cagcaaaatt
     3901 gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc tggcaaaatt
     3961 gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa gctgacgctc
     4021 cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca tacgcgttca
     4081 atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa ctgcgttccc
     4141 tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc caaatcagca
     4201 tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg tagcaccacc
     4261 agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa acgctgaata
     4321 cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga cggcgcacct
     4381 aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag atcgcgcgcc
     4441 cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag actgcgctgc
     4501 aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat caacaacgcc
     4561 atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca ctcgaccaac
     4621 gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg cgcattgcgg
     4681 ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc aagctccgtg
     4741 agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca tcgtagacat
     4801 gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg atatcaagac
     4861 gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga atccctaccg
     4921 ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca cgctcataaa
     4981 cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg gcgcgctccg
     5041 gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg gtatcgacat
     5101 catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat aagattgtct
     5161 gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac ggcacggcgg
     5221 aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg gtgacggtca
     5281 gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc agtcctttca
     5341 gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact tcactgccat
     5401 cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc tcaaccacca
     5461 gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc agctccggtg
     5521 aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca aggccatgca
     5581 ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac ttttagtaat
     5641 tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc gtggattgcg
     5701 tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc gggaaataga
     5761 gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca gtgagtgcct
     5821 taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct ttctatcatc
     5881 gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga aaagtgagga
     5941 aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag tttcgtgatc
     6001 aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt
     6061 tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca taatatttac
     6121 cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa tctttctcta
     6181 cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga cccgacaaca
     6241 ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta cctctatgag
     6301 ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa tatcctctac
     6361 gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag
     6421 gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa gcgcaccgat
     6481 gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga attccggcct
     6541 ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt gtatatcaat
     6601 gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag
     6661 cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg cccctgggat
     6721 accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga tgccgacccg
     6781 gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg tgataaaaac
     6841 gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga tttaacaaag
     6901 cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt
     6961 aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag tgatgctgtc
     7021 gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa ccgtaagctg
     7081 gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa cgccgggaaa
     7141 cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg tttacacgtg
     7201 tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga acgcgaagcc
     7261 catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg ctacatttac
     7321 agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca agttcgctta
     7381 atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac gggatatctg
     7441 gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa
     7501 tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt ctgacgcctg
     7561 ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct tacggtaaag
     7621 gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg gccggtttgc
     7681 gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa ataagcatag
     7741 aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg
     7801 ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct atgggccgct
     7861 tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt aatagcggcg
     7921 cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc acgctggtgg
     7981 atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat gttactgtat
     8041 tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga aattcatatt
     8101 ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt caccttacgg
     8161 cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg gtttttcgcg
     8221 acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg atacccgtag
     8281 atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc
     8341 ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa c
//
LOCUS       DAJTNP010000147         8132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTNP010000147 DAJTNP010000000
VERSION     DAJTNP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..214)
                     /locus_tag="OO160_003718"
     CDS             complement(<1..214)
                     /locus_tag="OO160_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR5525681.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWA"
     gene            complement(211..1089)
                     /gene="xdhB"
                     /locus_tag="OO160_003719"
     CDS             complement(211..1089)
                     /gene="xdhB"
                     /locus_tag="OO160_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5525682.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1100..3397)
                     /gene="xdhA"
                     /locus_tag="OO160_003720"
     CDS             complement(1100..3397)
                     /gene="xdhA"
                     /locus_tag="OO160_003720"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5525683.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            3812..4567
                     /locus_tag="OO160_003721"
     CDS             3812..4567
                     /locus_tag="OO160_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5525684.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4646..4719
                     /locus_tag="OO160_003722"
     tRNA            4646..4719
                     /locus_tag="OO160_003722"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4678..4680,aa:Gly,seq:ccc)
     gene            4854..6464
                     /locus_tag="OO160_003723"
     CDS             4854..6464
                     /locus_tag="OO160_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5525685.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6496..7103
                     /locus_tag="OO160_003724"
                     /pseudo
     CDS             6496..7103
                     /locus_tag="OO160_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8034..>8132
                     /locus_tag="OO160_003725"
     CDS             8034..>8132
                     /locus_tag="OO160_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5525686.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHV"
BASE COUNT         2079 a         1981 c         2085 g         1987 t
ORIGIN      
        1 cagcccaggc ggcaaggtat aagcaactgt ctattgctgt gccgtcgacc aacaccgtac
       61 aggcaccaca ttcacccacg cagcaccctt gtttgacact tagcagtcct tgttcgcgga
      121 gtaattccga gagcggcgtg cctggtgcgg cgtgaagctg aaaaggcatc ccgttaatgg
      181 tgcattcggt ggtaattgtt tcgctgtgat tcattgcaat tttccccccg ccgcggcgac
      241 ggcttcgcta atcacttttt tggtcatcgt ctggatgaga tgcagacgaa actctttact
      301 ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca gattcgctga tagcttccag
      361 cgtttgcagg tttaatggcg cattttgtgc agtctgttcg gcatgttggc agcgaatcgg
      421 cgttggcgcg gcaacaccaa atgccaggcg taattcgctg aaattgccgt tatccagtcg
      481 gcaatgtgcg gcgcagccaa tcgttgaaat atccattgcg tcgcgcatgg catatttaaa
      541 atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa tgaaaggcga cgaggatttc
      601 gtcatgctca agagacactt tgcccggccc ggtgtgaaag ccattaatcg ggacgaaacg
      661 aacaccgcgc ggggagtgga tctccagttt cgcgtcataa attagcgttg gcgtggcaga
      721 atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag gtagcgacgt tacggatctg
      781 cggtccagca atgaacgtgg ccgcagcaca taacgccggg agatgacgtt gagttatagg
      841 atcttctatt agctgggtaa atgtcgttgc agagccgata cgtagcgagc catcttccgc
      901 cagcgtaatt ccccgcagct ccgccagatt atgaatatca acaatatggc gataacggtc
      961 attgtgatgg tggagctgaa tcagtacgtc agtgccaccg gcgagcagtt tggcctgcgg
     1021 gttgtcagcc agcaggttga tggcatcggc aagggttgct gcgcgatggt aagaagcaaa
     1081 atcaaacatg atgttatcct caaatcaatc ctgccagatg gaactcttca tataaccgtt
     1141 ttggcgtcag cggcagtgta ttgattgcaa caccggtagc catcttcacc gcgttacgaa
     1201 tagcagcggc aacaggaatt atgggtggct cacccagtga cttatgtccg tatgcggatt
     1261 gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg cagatccggc atggtcggca
     1321 ttttgtaatc cagcagattg gggttacgga ccacgccgct tttagcatcg atgatcatct
     1381 cttcaaatag cgcccagcca atgcccattc ccattccgcc gtgtacctga ccttctgcca
     1441 gcagtggatt aagaatatgc cctgaatcat gaacgttgag gatgcggttg atggtgactt
     1501 tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca gccaaacgcc ggtgggttag
     1561 tggtggtttt gatggagctt tcagcagaga gctgcccgcc gcgttcaggg tggtagaaag
     1621 cgtccatcgc caaatctttt aacgacatta acggctcttc cggtcgttca accagcacga
     1681 tatggccttt tatcagggtc aaattcatcg ctgactgatg tagcatgact gcggcgtgag
     1741 cgatgatttt ctcttttaat aatagtgccg cactgcgcag cgcaggcgcg gcaacatagc
     1801 tctggcgtga ggcaaatgcg ccgggatcga acggcgtaac gtcggtatct tgtgttgaaa
     1861 taacgcgaac gtcgctgacc ggaaccccca cggtttctgc caccatttgc gagaagacgg
     1921 tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac gttgatggtt ccatcctgat
     1981 tcatcagaag gcgcgcgcct gctatttcta cgccgacagg ccaggtgtta gaggtgtagc
     2041 taaaacaggc gacgccaacg ccgcggcgca aattgccttg ctggttctgg cattctgcac
     2101 ggcgtttttc ccattcaaag attttccggc ctttttcaag acactccggc aaccctgcgc
     2161 tgcaaatacg tttgcccgtg agcggattag catctccttc gcgggcggcg ttgcgtaaac
     2221 gaatttcaac aggatcaata cctaacgctg tcgcggcgtc atcaagcata gactcaacgg
     2281 caaatacgac ttgtggcgcg ccataaccac gcatcgcacc agccgagggg aggttggtat
     2341 agcaggtctt tgaactgtaa gcgtaggcac aacgaggata aaggtaagcg actttattcc
     2401 cccccgcaga agcgatggag tgcccgtgag atgcataagc gccggtgtta gacagaacat
     2461 ccagactata acctttcaag gttccgtcgc ggttcacgcc catttgcccg tcaatagtaa
     2521 aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg gctaagggaa actttcaccg
     2581 gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg ctcttccagt acatcctgtt
     2641 tattaccaaa accgccaccg acaaacggtt tgatgactcg tacgcatgac cagggaatat
     2701 ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat ctgggtgctg gaaacgatgg
     2761 taattcgcga gtcatcctcc atccacgcca gcgatgttac gctttccatg tgacaatgtt
     2821 gaataacggg agtctgatag tgcccctgta cctggtagtc ggcggcatcg attgtttgtt
     2881 ggacattgcc cgtcgacatc gtgctttgtt tcagtaaatt gccgccgtta tggattggtg
     2941 cagcgtcttc tgtcagcgcc gcttctggcg tggtgataac gggtaattct tgccactcaa
     3001 tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc gcgggccacg acgatggcaa
     3061 cggcgtcacc atgatgacga acatggcgag ttagcagtgc gcgatcggcg gtatcgcgct
     3121 tgttttcgtc aagtgtccag gcatgcccag ctgtagcgaa tggaatatca ggcacatctt
     3181 cccaagtaaa aatcgccagt acgcccggta aacttctggc ttgttcatca ttaatactta
     3241 cggcataacc atgtgcgata gggctacgta catatttcgc gtaacacatg cccgccataa
     3301 cataatcgtc agtatatcgt gcccgcccgg tgaccttagc aatggcatcg acgcgcatgc
     3361 atgattcacc cgtagcggtt gcttcccgcg cttccataga aatcccctcg atttgaacat
     3421 ttggttctta tggcaaagat ttagtctaag caggcaagat ttacgccaga aacgacgata
     3481 agcgttaggg gaggaaggaa gtgtgatgac gttaaaaaaa cacagatatg atacgaaaca
     3541 ggactctgaa tagggacagt gatacgtatc aatatgagtg gcgataaaat caatgtgata
     3601 aatcaggatt attacgcatc aggatgggaa atagggtaaa aagtcaggaa cttcgatacc
     3661 tctacagcga atattttgct gatgatactt ccctttgcca aatgaaatta acctctttct
     3721 ctattaccac gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata
     3781 tggcgtcttg ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc
     3841 tctgggtatc gtgatgtttt tatcggttgg actgctattg gcgggctgtt cgggtagcaa
     3901 atcatccgat acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct
     3961 atatcgtatt tcgcgcacca cgggaaccag cgtaaaagag ctggcgcgac tgaacggcat
     4021 ttccccccct tacaccattg aagttggtca gaaactaaaa ctgggtgggg cgaaaagtag
     4081 cagtagtaca cgtaaatcaa ccgccaaatc aacgactaaa accgcatcgg ttacaccgtc
     4141 atcagcggta ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac
     4201 gacagggaaa gttatcatgc cgtattcgac agcagatggc ggcaataaag ggattgatat
     4261 ctcagctcca cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg
     4321 caaccagctg cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac
     4381 ggcttacgcc cataatgaca cgatgctggt aaataatggg caaagcgtga aggctgggca
     4441 aaaaatcgcc actatgggga gcacggatgc ggcatctgtt cgcctgcatt tccagattcg
     4501 ttaccgtgca acggcaattg atccgctacg ttacttgccg ccgcagggca gcaagccaaa
     4561 atgctgatgg cgaattaatc agcagtcagc agcgcggcac ttgctaagga gagcgtaagg
     4621 tttataatgc cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc
     4681 aagctctata cgagggttcg attcccttcg cccgctccat gacacttctt aaagtctact
     4741 aacttactga atcctaaggg atacggctta acgccttccg atagttttac tcactgtaac
     4801 caacactgtg actaactttg ttgaagtagt gagcagacct aaggtctcag tacatgctca
     4861 agtcaagaac ctacctttat cagcgtaatg gtgtctttta catccgtctt aggatgaaga
     4921 ccactagtcg cctgaccgct tcgcttcctt ctcacaaccg ttataaacta gcgtcagtat
     4981 ctttacggac caaggataga cgtaccgcta tggctcactc acggcacatc aagtcagcac
     5041 ttaaagcaat ccatgcagac aaccctaacg cctcttatga ggagctacgg gagcacctga
     5101 agaccattgt cgagtgggaa cttagtgtca gccgtgatga cctgaacgac ccggagtcct
     5161 accagcttta cgttgaccag tacgatgaca ttaagtccaa ccttcgggaa gctgtagcga
     5221 ccgagcgtct gactgtagac cagcatcgct atattaatga cgttatcggt gtgcttaagg
     5281 cctgtcagga tagactcaat ggcgatagct ctggcctatt gtcttacctt gagcctgaga
     5341 ctggcagtct aagaccgtcc gtctccctat ctgtattggc ggaacctgaa gtccctgaac
     5401 ctaaagcact gaccctcgcg tctctcattg agcagtacga gcaggagaac gcccagaact
     5461 ggaagccagc gaccctaagc gaaaaccgag cgtcacactc tacgctaatc gagatattcg
     5521 actatctgga tattcaggat gtcggcaagg cgacccgtgc agatatgctc agagttcgtg
     5581 aagtcctcca gcagctaccg aagaaccgca agcaacgctt taagtctatg ccgctgtctg
     5641 acttgctgaa ccgggagtct aagactgact gtctggacgt cgttaccatt aacaataaat
     5701 atcttatcaa gatggctgct gtgtttaagt gggcggtacg caacgacttg atagctaaga
     5761 acctgaccga aggtcttgag ctgaaagtcc ctcagcgtaa agcctccgac gctcgtgatg
     5821 cgttcagtcc tgagcaggta gggcaactac tggtcgcagc taaagcgtac tcgcagaaga
     5881 cctcaggtaa gccataccat tattacgtca ccgctctggc tgcgattaca ggtgcgagac
     5941 ttaacgaggt ggcccaactt caggttaaag atgtcaggac cactgaggct gggaccgttt
     6001 ttatccatat caacgaagac gatagcagcc tgccgggtaa gagtatcaag aacgcccaca
     6061 gtgaccgctg tgtcccgttg gtagatggtg cttatggttt tgtattggct gactttatgt
     6121 cactcgtgga agaccgtaga aagactgagg gtgataatgc tatggtgttc aatggcctca
     6181 agctaatgaa gaacggctac ggtgagcagg ttagcaagtg gtttaaccga acgttgctgc
     6241 ccaaagtcct cgctgaccgt agcggcttag cttttcactc gtttagacat acagttgcga
     6301 cccagttaaa acaacatggg gtcgagttag cgtatgctca ggcgattatg ggtcatagct
     6361 cagggtctat aacttacgac cgttacgcga aagaggtcga ggtggaaacg ctgaaagaaa
     6421 aattagcgga gtcattgtca gtaaaaaaaa tagacggaaa gtaatcgcat agggatagcg
     6481 gaatgtgagg acgtaatggt caaagaattt ttagttagca aagaaaataa gccttcatat
     6541 gaatgtatga atactgctga aacttgtttg gggctggctg gaatggcttc ggaagattgg
     6601 caacgtgagc atcattgccg cacaggtata atatttactg cttttgctat tgaggcaatg
     6661 ttcatcttct atagaaaaca ggttgacccc ggctacgata agacgcagaa agagtgcaga
     6721 aagacaatgc acaagaatac gttaaaattg tgcggtataa acaattatat ggggacaaaa
     6781 ccttaccaaa ttatcaaaga gtgtcttgag gtaagagacg ctattgctca cggcgattcg
     6841 tatacttcga gctttaattt ctcggcggat catttggata accaagacga tattgcaaaa
     6901 gtgtcctggc ggtaaactct gaacaatttc ggggattaac tgttgagcgc cttgcaaaat
     6961 acattgaaat ggcaaagcat attgaccatg agatttctga taatggttac aggccttctg
     7021 aaggttacct tccagcagcc gagcgcagaa gtctgatgtt agcttttggc gtcagtggtg
     7081 tgtcaatgtg gcccagtgct tgagcgataa tacacctcac tatagaggaa tggaccctta
     7141 actatcacta taggggagag accctaagtc cccaccttaa tagcagtcac gacccataca
     7201 cacattcatt aagtctcagg cttccggtag cggaccgatt ggaaaccatc cgcagacgcc
     7261 tagtgttaaa tcaccttggc ctgagaccat tccttttaac gtaagctatc ttcccttttg
     7321 acaggcgggg agcagtagac cacatccgac tttaagttgg ctctgtgata atcgctgcgc
     7381 ggataactga agaaccaaga caccattact gaggtctgcc atccatttgt ggacaggcgg
     7441 aaactcaaag aggttagatc agtggtgtct gaggttggag ctttgagtct tgaccagcag
     7501 gctggaggag accatgagtt tatcttgtta tggattgtgt tatcacaatt ccaataagaa
     7561 gaactttaag tccagcctcc actcccgcca acccgaagta aaattatggt cgctatgatg
     7621 tggtctccat gcttcgggtc ttactggaag tactgaacac tttcgcgtca agttctccga
     7681 agttcattcc tgtttgacta aactatcact atagggacca tatctcgcct ctgacctcct
     7741 gagagacggc aagcgagaaa ccgtggtcca cgctagcaag ctagccacca gcctaaggca
     7801 agacagcctc tggtcagcct gtgtgttctt tggtctcgtg acttaagtct atcaccacac
     7861 acgctcggac atgctgtcga accttcatct ctatggtcat cgcagaccac taaccccaat
     7921 tacctcaagt cattctgttt gcgtcctgtt gcctgctggt ctccctttac gtgggggctt
     7981 gagtaggtca gtggggtgcg acttcagggt gcaaacaaaa ggagtacacg taaatgtccc
     8041 aacaagaaat cagcatcatt aacctcgacc agcttgtgtc catgacctca gttgaaattg
     8101 ctgagctgac aggaaaagaa cataaacacg tt
//
LOCUS       DAJTNP010000148         8094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTNP010000148 DAJTNP010000000
VERSION     DAJTNP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..851
                     /locus_tag="OO160_003726"
     CDS             <1..851
                     /locus_tag="OO160_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5525687.1"
                     /translation="AIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGKSGAGKSSL
                     CNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRD
                     ILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGI
                     QPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLM
                     TRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAV
                     WNWIFF"
     gene            1185..4307
                     /gene="ag43"
                     /locus_tag="OO160_003727"
     CDS             1185..4307
                     /gene="ag43"
                     /locus_tag="OO160_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5525688.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            4428..6944
                     /locus_tag="OO160_003728"
     CDS             4428..6944
                     /locus_tag="OO160_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525689.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            7020..7475
                     /locus_tag="OO160_003729"
     CDS             7020..7475
                     /locus_tag="OO160_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525690.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(7594..7805)
                     /locus_tag="OO160_003730"
                     /pseudo
     CDS             complement(7594..7805)
                     /locus_tag="OO160_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7886..>8094
                     /locus_tag="OO160_003731"
     CDS             7886..>8094
                     /locus_tag="OO160_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5525691.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTR"
BASE COUNT         1926 a         2139 c         2268 g         1761 t
ORIGIN      
        1 aggcaattga aaaaccgctc tcctccctgc cttactcact ttcccgtcac atcctggaac
       61 atctgcgcaa actcaccagt cacgaacccg tgattggcat tatgggtaaa agcggggccg
      121 gtaaatcttc actctgtaat gcactgtttc agggggaggt cacaccggtc agtgatgttc
      181 acgccggcac ccgggaagtg cagcgcttcc gtctgagtgg tcacggtcac agcatggtta
      241 tcactgacct gcccggggtg ggcgagagcc gggacaggga tgcagagtat gaagccctgt
      301 accgtgacat tctgcctgaa ctggacctgg tgctgtggct gattaaagcc gatgaccggg
      361 ccctgtctgt ggatgagtat ttctggcgac acatcctgca gtgcggacat cagcaggtgc
      421 tgtttgtggt gacgcaggct gacaaaacgg aaccctgcca tgaatgggat atggcaggta
      481 ttcagccttc tcccgcacag gaacagaaca ttcgtgagaa aacggaggcg gtattccgtc
      541 tgttccggcc cgtacatccg gttgttgccg tatcggcccg caccggctgg gaactggata
      601 cgctggtcag tgcgctcatg acagcgcttc ccgaccatgc cgccagcccc ctgatgaccc
      661 gactgcagga cgagctgcgc acggagtctg tccgctctca ggcccgtgaa cagtttaccg
      721 gtgcggtgga ccggatattt gacacagcgg agagcgtctg tgttgcctct gttgtccgta
      781 cggccctgcg cgctgttcgt gacaccgtgg tctctgttgc ccgcgcggta tggaactgga
      841 tcttcttctg aacctgttgt ggatgatgtc ctccctgcct ctgagtctgc tcacaaaagc
      901 gctgttttcg ttactgtctc tcttgtccgt gcaatagact gataatagaa taaaacgatc
      961 gatacctatt ttaccgatcg ttaatatcga tcgttatgct aataataact cctgttagca
     1021 acgtgcgcag atacacacag acatgagatt cagggaacaa cagagccaca cgtcagaaac
     1081 ttccgtcagc cggacctccg gcactgtaac cctttacctg ccggtatcca catctgtgga
     1141 taccggcttt tttattcacc ctcactctga ttaaggaaat gctgatgaaa cgacatctga
     1201 atacctgcta caggctggta tggaatcaca ttacgggcgc tttcgtggtt gcctccgaac
     1261 tggcccgcgc acagggtaaa cgtggcggtg tggcggttgc actgtctctt gccgcggtca
     1321 cgtcactccc ggtgctggct gctgacatcg ttgtgcaccc gggtgaaaca gtgaatggcg
     1381 gaacactggt aaaccatgac aaccagtttg tatccggaac agctgatggc gtgactgtca
     1441 gtaccgggct tgagctgggg ccggacagtg acgaaaacac cggcgggcaa tggataaaag
     1501 cgggtggcac aggcagaaac accactgtca ccgcaaatgg tcgtcagatt gtgcaggcag
     1561 gaggaactgc cagtgatacg gttattcgtg atggcggagg gcagagcctt aacggactgg
     1621 cggtgaacac cacgctggat aacagaggtg agcagtgggt acacggggga gggaaagctg
     1681 ccggtacaat tattaaccag gatggttacc agaccataaa acatggcgga ctggcaaccg
     1741 gaaccatcgt caacaccggt gcagaaggtg gtccggagtc tgaaaatgtg tccagcggtc
     1801 agatggtcgg agggacggct gaatccacca ccatcaataa aaatggccgg caggttatct
     1861 ggtcttcggg gatggcacgg gacaccctca tttacgccgg tggtgaccag acggtacacg
     1921 gagaggcaca taacacccga ctggaggggg gtaaccagta tgtacacaac ggtggcacgg
     1981 caacagagac gctgataaac cgtgatggct ggcaggtgat taaggaagga ggaactgccg
     2041 cgcataccac catcaaccag aaaggaaagc tgcaggtgaa tgccggcggt aaagcgtctg
     2101 atgtcaccca gaacacgggc ggtgcactgg ttaccagtac agctgcaacc gtcaccggca
     2161 caaaccgcct gggagcattc tctgttgtgg agggtaaagc tgataatgtc gtactggaaa
     2221 atggcggacg cctggatgtg ctgaccggac acacagccac taatacccgc gtggatgatg
     2281 gcggaacgct ggatgtccgc aacggtggca ccgccaccac cgtatccatg ggaaatggcg
     2341 gtgtactgct ggccgattcc ggtgccgctg tcagtggtac ccggagcgac ggaaaggcat
     2401 tcagtatcgg aggcggtcag gcggatgccc tgatgctgga aaaaggcagt tcattcacgc
     2461 tgaacgccgg tgatacggcc acggatacca cggtaaatgg cggactgttc accgccaggg
     2521 gcggcacact ggcgggcacc accacgctga ataacggcgc catacttacc ctttccggga
     2581 agacggtgaa caacgatacc ctgaccatcc gtgaaggcga tgcactcctg cagggaggcg
     2641 ctctcaccgg taacggcagc gtggaaaaat caggaagtgg cacactcact gtcagcaaca
     2701 ccacactcac ccagaaagcc gtcaacctga atgaaggcac gctgacgctg aacgacagta
     2761 ccgtcaccac ggatgtcatt gctcagcgcg gtacagccct gaagctgacc ggcagcactg
     2821 tgctgaacgg tgccattgac cccacgaatg tcactctcgc ctctggtgcc acctggaata
     2881 ttcccgataa cgccacggtg cagtcggtgg tggatgacct cagccatgcc ggacagattc
     2941 atttcacctc cacccgcaca gggaagttcg taccggcaac cctgaaagtg aaaaacctga
     3001 acggacagaa tggcaccatc agcctgcgtg tacgcccgga tatggcacag aacaatgctg
     3061 acagactggt cattgacggt ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg
     3121 ccggcaacag tgcgtcgggg ctggcgacca gcggtaaggg tattcaggtg gtggaagcca
     3181 ttaacggtgc caccacggag gaaggggcct ttatccaggg gaataagctg caggccggtg
     3241 cctttaacta ctccctcaac cgggacagtg atgagagctg gtatctgcgc agtgaaaatg
     3301 cttatcgtgc agaagtcccc ctgtatgcct ccatgctgac acaggcaatg gactatgacc
     3361 ggattctggc aggctcccgc agccatcaga ccggtgtaag cggtgaaaat aacagcgtcc
     3421 gtctcagcat tcagggcggt catctcggtc acgataacaa cggcggtatt gcccgtggag
     3481 ccacgccgga aagcagcggc agctatggct tcgtccgtct ggagggtgac ctgctcagaa
     3541 cagaggttgc cggtatgtct gtgaccgcgg gggtatatgg tgctgctggc cattcttccg
     3601 ttgatgttaa ggatgatgac ggctcccgtg ccggcacggt ccgggatgat gccggcagcc
     3661 tgggcggata cctgaatctg atacacaacg cctccggcct gtgggctgac attgtggccc
     3721 agggaacccg ccacagcatg aaagcctcat cggacaataa cgacttccgc gtccggggct
     3781 ggggctggct gggttcgctg gaaaccggtc tgcccttcag tatcactgac aatctgatgc
     3841 tggagccgca actgcagtac acctggcagg gactctccct ggatgacggc caggataacg
     3901 ccagttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggc ttccgtctgg
     3961 gcagccacca cgatatgaac tttggtaaag gcacctcatc ccgtgacacc ctgcgcggca
     4021 gtgccaaaca cagtgtgcgt gaactgccgg tgaactggtg ggtacagcct tctgttatcc
     4081 gcaccttcag ctcccggggg gacatgagca tgggtacagc cgcagccggc agtaacatga
     4141 cgttctcacc gtcacagaat ggtacgtcac tggacctgca ggccggactg gaagcccgtg
     4201 tccgggaaaa tatcaccctg ggcgttcagg ccagttatgc ccacagcatc aacggcagca
     4261 gcgctgaagg ttataacagt caggccacac tgaatgtaac cttctgataa ttctgcattg
     4321 cctctctgtg gtcccggtca tgatgaccgg gacccggacg ggggaaaacg tttcagtgcc
     4381 acattcactg gcattcacaa taacatgata ttcatcacgg agtgactatg ttacagatag
     4441 tcggcgcgct gattctgctg atcgcaggat ttgccattct tcgccttttg ttcagagcat
     4501 taaccagcac agcgtctgcg ctggcagggt tcatattgct gtgtctgttc ggtccggctt
     4561 tactggctgg ctatatcact gaacgcataa cccggttatt ccatattcgc tggctggcag
     4621 gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt gatggtaaac
     4681 atatcgcact ggaggctcat acttttgact ctgtaaaatt tattctgacc accgctctcg
     4741 ccgctggtct gctggctctt cccgtgcaga taagaaccat tcagcagaac gggctcacac
     4801 ctgaagatat cagcaaggaa attaacgggt attactgctg tttttatact gcttttttcc
     4861 ttatggcgtg ttctgcatac gcaccattga tcgcattgca gttcgatatt tcaccctcac
     4921 tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg ctatgggcgg
     4981 ccagccagat ccaggcgctg aaaagactga ccagtgccat cagccagaca ctggaagaac
     5041 aaccggtgct caacagtaaa tcgtggctga gcagtttgca aaacgattac agccttcctg
     5101 aaacgctgac ggagcgcatc tggctgacac tcatttcaca acggatttcc cggggagaac
     5161 tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg tatgaaagaa
     5221 acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct gatgaactga
     5281 aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgacgat tttctcgatc
     5341 gttgcctgga cggcggtgac tggtacccct tttcagaagg ccgccgtttt gtatcattcc
     5401 accacgtgga tgagcttcgt atctgtgcct cctgcgggct gacagaagta catcatgccc
     5461 cggaaaatca taagccggct ccggaatggt actgctcctc tctttgtcac gaaacagaaa
     5521 cactgtgtca ggacatttat gaacgttctt acaccggttt tatttccgat gcaacggcga
     5581 atggtctgat tctcatgaaa ctgccggaaa cctggagtac aaatgagaaa atgtttgctt
     5641 ccggagggca gggacatggg tttgccgctg aacggggaaa ccatattgtc gacagagtcc
     5701 gtctgaaaaa cgcacggatc ctcggtgata ataatgccag aaatggagca gacagactgg
     5761 tcagcggaac agaaatccag acgaaatatt gttcaactgc agcccgtagc gtcggtgcgg
     5821 cattcgacgg acaaaacgga cagtatcgtt acatgggaaa tcatggtccc atgcaactgg
     5881 aagtcccccg tgatcagtat gccggcgctg tggaaaccat gaagaataag atccgcgaag
     5941 gtaaagtacc cggtgtaacc gatcccgcag aagcgtcccg gctgattcgt cggggacatc
     6001 tgacttatac ccaggcccgt aatatcaccc ggttcgggac catcgaatcg gtcacttatg
     6061 atattgccga ggggtcggtt gtcagtctgg cggccggagg gatcagtttt gccctgacgg
     6121 catcggtctt ctggctcagc accggcgatc gcgatgctgc cctgcagaca gctgctgtcc
     6181 aggcaggaaa aaccttcacc cgcacactgg ctgtctacgt cacaacccag caacttcacc
     6241 ggctcactgt tgttcagggt atgctgaagc atattgattt ttcgacggcc agcccgactg
     6301 tccggcaggc gcttcagaag gggaccggtg caggaaatat cagtgccctg aacaaagtga
     6361 tgaagggtac gctggtgaca tctctggcac tggtagctgt cacaaccagc cctgacatga
     6421 tcaaaatgtt gcggggacgg atctccggtg cgcagttcat caggaatctt gccgtggcat
     6481 cttccggtgt ggcaggtggt gctgtcgggt cagtggcggg cgggatattg ttcagtccac
     6541 tgggaccatt tggtgcactg acagggcgtg tggttggcgg tgttctgggg ggaatgattg
     6601 cctccgctgt atcaggaaaa attgccggag cgctggttga agaagatcgc gtcaaaattc
     6661 tggcaatgat tcaggagcag gtgacatggc ttgccggcag tttcctgctg accggacatg
     6721 agattgaaaa tctgaacgcg aatctggccc gtgttatcga tcagaatgct ctggagatca
     6781 ttttcgccgc cggtatacaa caacgggccg cgaccaatat gttaatcaaa ccactggtgg
     6841 tcagtatcat caggcaacgc cccgtcatgg aatatgatgc atcccatctc ggcaagatgg
     6901 ttaaccggct ggaagaagca ttccccccgg agctcccggc ataacgtgtg ttctgctcct
     6961 tttaggtttt atcacctgat ttgccagtgt catcgtcatg actcagagag gtaaatacca
     7021 tgattcacct gttcaaaacc tgcatgatta ccgccttcat tctggggtta acgtggtctg
     7081 ccccactccg ggcacaggat caacgttaca tcagtatacg caatacagat acgatatggc
     7141 tcccgggaaa tatttgtgct taccagttcc ggctggataa tggcggaaac ggtgaaggat
     7201 ttggccccct caccatcact ctgcaactca aagacaaata tggtcagacg ctggtgacca
     7261 gaaaaatgga aacggaagct tttggtgaca gtaatgccac gcgaaccaca gacgcatttc
     7321 tggaaacgga gtgcgtggaa aatgtcgcca caaccgaaat cattaaagca actgaagaaa
     7381 gtaacggcca tcgtgtcagt ctgccgttat cggttttcga tccccaggac taccatccac
     7441 tgctgattac cgtttccgga aaaaatgtta actgatccct gaagtcagtt tcatgcttca
     7501 gcctctgcca caacgtatgg cagaggctat ttatcaggag aaaccagata tgccgggtta
     7561 cacacagagt atgtcctggc tgaaggatct ttctcatacg ggcaggcggt tgctgtcata
     7621 accgcttacc gcaatgtgtt tatccaggat gacccgggaa tgcatttccg tcgggttatc
     7681 cgcaacgcgg aaggtcagcg acgctgacgc agccggaact ctgaagctga tgccggaaaa
     7741 cagcttaatg cgtggctcgc cagtggaggg cttctcagac aataaacttc attgatttca
     7801 tccatcaggc cgcgtcttct ccgggagacg cggctttttt catttgttgc accagtaaat
     7861 cttaaccacc gataaggagc aaagtatgca attagtcagt cgttttggtt atgcgaacca
     7921 gatacgccgt gaccgtccgc tgacacatga agagctgatg caccatgtgc cggggatttt
     7981 cggggaagag aaacacacgt cccgaagcca gaactatacg tacatcccca ccatcaccgt
     8041 actggaaagt ctgcagcggg aaggcttcca gccctttttc gcctgccaga cccg
//
LOCUS       DAJTNP010000149         8013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTNP010000149 DAJTNP010000000
VERSION     DAJTNP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="OO160_003732"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="OO160_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO160_003733"
                     /pseudo
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO160_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO160_003734"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO160_003734"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5525692.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO160_003735"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO160_003735"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5525693.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="OO160_003736"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="OO160_003736"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5525694.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7784..7859
                     /locus_tag="OO160_003737"
     tRNA            7784..7859
                     /locus_tag="OO160_003737"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7817..7819,aa:Gly,seq:gcc)
BASE COUNT         1830 a         2069 c         2170 g         1944 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 tcagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
     7801 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa
     7861 aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag
     7921 gcggcaactg aacgaatcct tgggaactta caaaagtatg tgactaaggt gagtgaaggc
     7981 agccaaggca gtagcggttc gaaagacgaa gat
//
LOCUS       DAJTNP010000150         7981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTNP010000150 DAJTNP010000000
VERSION     DAJTNP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO160_003738"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO160_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO160_003739"
     CDS             1127..1234
                     /locus_tag="OO160_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525695.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO160_003740"
     CDS             complement(1287..2045)
                     /locus_tag="OO160_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5525696.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO160_003741"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO160_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5525697.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO160_003742"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO160_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5525698.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO160_003743"
     CDS             3758..4618
                     /locus_tag="OO160_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5525699.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO160_003744"
     CDS             complement(4659..5195)
                     /locus_tag="OO160_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5525700.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO160_003745"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO160_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5525701.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO160_003746"
     CDS             6173..6763
                     /locus_tag="OO160_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5525702.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..7981
                     /gene="tcyP"
                     /locus_tag="OO160_003747"
                     /pseudo
     CDS             6896..7981
                     /gene="tcyP"
                     /locus_tag="OO160_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2098 a         1902 c         1893 g         2088 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctagacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 g
//
LOCUS       DAJTNP010000151         7980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTNP010000151 DAJTNP010000000
VERSION     DAJTNP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..572
                     /locus_tag="OO160_003748"
     CDS             <1..572
                     /locus_tag="OO160_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525703.1"
                     /translation="TGTPATDLDDDYFDMLQEELCSVVEASGASLEKGRHDQLLTALR
                     ALLLSRKNPFGDIKSDGTVKTALENLGLGEAAKRNVGTGANQIPDMSLFASINTVTAA
                     AQKFPSGLILQCGQLNGAPNVSSTYGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATS
                     VQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            544..978
                     /locus_tag="OO160_003749"
     CDS             544..978
                     /locus_tag="OO160_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5525704.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1751..2800)
                     /locus_tag="OO160_003750"
     CDS             complement(1751..2800)
                     /locus_tag="OO160_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5525705.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINAINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3251..4441
                     /locus_tag="OO160_003751"
     CDS             3251..4441
                     /locus_tag="OO160_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5525706.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4831..6291)
                     /gene="gtrII"
                     /locus_tag="OO160_003752"
     CDS             complement(4831..6291)
                     /gene="gtrII"
                     /locus_tag="OO160_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5525707.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6288..7217)
                     /locus_tag="OO160_003753"
     CDS             complement(6288..7217)
                     /locus_tag="OO160_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5525708.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7214..7576)
                     /locus_tag="OO160_003754"
     CDS             complement(7214..7576)
                     /locus_tag="OO160_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5525709.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2466 a         1512 c         1612 g         2390 t
ORIGIN      
        1 agactggcac gcctgccacc gatctggatg atgactactt tgacatgttg caggaggagc
       61 tttgcagcgt ggtggaggca tccggtgcca gcctggagaa ggggcggcac gatcagttgc
      121 ttaccgcact tcgcgcgctg ctgttaagcc gcaagaatcc gtttggcgat atcaaatcgg
      181 atggcacggt gaaaacggct ctcgaaaacc ttggtttggg agaagcagct aaaaggaatg
      241 taggtacagg ggcgaatcag atacctgata tgagcctgtt cgcgtcaatt aataccgtaa
      301 cggctgctgc gcaaaaattt ccgtctggat taattttaca gtgcggtcag ttgaatggtg
      361 ccccgaatgt atcttcaaca tacgggatga ggttcccgat gacgttctca agagtaattg
      421 ctgtcgtagt tacattgaac gttactggcg cggctgggca gccgactgta tcggcgacaa
      481 gtgtccagaa cactggattt aatattactg tgtcgcccgg ttcaggatac ggttcatctg
      541 ctgatgcgta ttacattgca atgggatatt aacaaaatgt catattttta ttctgcatcg
      601 acaaacggat tttattcgac tgaatttcac ggcaccaata ttcctgatga tgcagtggaa
      661 atctcggaat cagagtggaa aacactgatt aatgcacaga gtgtaacaaa aatgattacc
      721 tgtggtgaga acggtcatcc tgtcattgtt gaccgtcctt ctccaacacc agaacaatta
      781 gccttaataa atgatgaaaa gaaatctgca ctgatagcag aggcaacgaa tgtaatagct
      841 ccgcttcagg atgcggttga tttaggtatg gcaacagatg atgaaacgaa actgttactg
      901 gcatggaaaa aatatcgggt gctgcttatg cgtgttaatg tagtaaaacc cgagtggcct
      961 atgcatccaa ataaatagac ttgttaatct aggataattg ttggaataat actcgttttt
     1021 cagtaggttc aggtagcctg ggtataatgc tcacttctac aatgatttca acttatttta
     1081 tttaattgat attacgaaac ttaactcata cataaacaat atgactcaat actgtcctaa
     1141 atagctgcgc agaaaagtgc tccactttcg ctcagccctt gctgggcgaa gcatcaggaa
     1201 ggcgttctca gctaggttgt gttatatctg gcttgagttt tgttgcccat acgcaacaca
     1261 ttacccatct gtaaacgaca agtgtggcag aattaggtgc agctatttag actatatcga
     1321 cgcagtaacg ataatagata tcgaaactgt actagatatt agctacttat ggttaccggg
     1381 gggagtcttt tgagcactgg tgttatcaat tggcttcaaa taattcgcca ataaattata
     1441 ttgcgatagc tcatcacctt ccaggttgtt tatagccagt ttatcagcgt caccacctac
     1501 tgtttatgct tttatttctt tgattaataa gtatatgata ttttaattca tctggtgtat
     1561 ttctttctca tgtgttggtc gactgaatat ctgttcagtg aaggggtaat tttatgtttg
     1621 cacttcgaac attcccttca gttgctagga aagaaaaccg taacatttgc gcattaattt
     1681 taacagtata atctgagcat aaatatgcgc tatatatcta taaaaaggcg caattaattg
     1741 cgccttttta tcattttatc aaaaatggcc caccgactgt cctcgatagc caatggccaa
     1801 ttttatctga tacatataca caaatacagg tatatatgat tgcgcagata ggatttggaa
     1861 tagtcacgct gaagcctata attgctaaag ccatagggat aaataatttg gtgatatact
     1921 ccattccaca tatgttaaga gtatttcttc cagctcttac aataaagtca tttccacaaa
     1981 ttatttttgc tatgaaaata ttaaagatta tcaagcctac cgtggtgata atagtattaa
     2041 tagcttgaat ataactactt gatattatgt ctgcattgaa tattgagata atcttaaaaa
     2101 gtaaaggttt ctggttaagt agttgatacg cactcattat agcggtaatt gagaagacaa
     2161 tgaaaccaat tttagatttt ccaaaaaacc tgtcacgtgt taattctaaa aacatacatc
     2221 taccgagagg caataaccac cagtatgcca tagcggagtc tatgttccaa aaccattgag
     2281 gatctagtaa tgggttatga ttgagcactg tttgtgacaa catgaacgat aacagtgata
     2341 tgattaaaat cactatattg ttcttgacta tttctctaaa gattgcatca attgctatta
     2401 taacaaaaag gcagtttata aaccatattg ttcctacgaa ttggttattt ctaacaccat
     2461 atattatatc ataaatgtgg ttgtatatat aatcaatcgt ctcacctgag ttaattgcgt
     2521 ttattattaa tatagaaaat gcaaaagtga aatatggaat cattaatctg taaaactttg
     2581 acttgatata atcgaaaaca gataagtcat tttttttgat agtaaaaaaa ccagcagcga
     2641 aaaagaatag tgggacgtga taactaaaaa caaaaggata caatttgcca gcccccaacc
     2701 caaggtgacc taaatatata gcaaaaatac cgagaaattt taaagcatca acccaatcat
     2761 gtcttatttt tctgttgctg tcgattttgt tattatacat ttaaagtagg ctctccagtt
     2821 ttgtgtgatt tttaaacact cattgcaaat atgaatgcct gaaagtttat gattattaat
     2881 caacgagttc aatattaatt tattattttt caaagtcgca ctatataggc atttacttcc
     2941 gcagtaccac cgatagtatc gatatcaaat cagcctgcgg ccgcgataac tttcgtaaca
     3001 ttctgccagc ggctttgttg aataaatcag gttcaagata atcgtctctg catcaggtta
     3061 gagtttcagg caatacatcc tctttctggc atacctgcca ctctcgcacc atggtcatag
     3121 cctttacgat ctgcatactc accagaccag agcccgctcc cttttaggct gaggaatccc
     3181 cagaaaagaa gacaggcata gagttttgtg atctactgat tgcgctaaca aattcaatga
     3241 gatcacctct atgcctgccc gtaaagtatg ccagactttt ttccgtaatg ctttagcttc
     3301 cacgcatcag tatcgacaaa atgccattat tgattctgct gctgctttag tcggtggcgc
     3361 atctctttct cttaccagca ttggccggca tttacccggt cctgctcgcg tgaaagataa
     3421 aataaaacgc gttgactgtc tgttgggtaa taaacgactt cataatgata tccctctgat
     3481 attcaaaaat attacatcca tgatgacaaa taaactgtcg tggtgtgtta tcgctgttga
     3541 ctggagcggt tacccttttc aggaatatca tgtccttcgt gccagcctcc tctgcgacgg
     3601 tcgctctatc cccctgatga gtcaggtctt cccgtcaaaa aaaaataacg aggcggtgga
     3661 aatcgctttt ctggatgcgc tttccggggc catttctccc cggactcgtg ttgttattgt
     3721 tactgatgct ggttttcaaa gtgcatggtt ccgtcatatc aaatcacagg gatgggattt
     3781 tattggtcgt atcagggggg tagtgaagtt caggctggat agtgacaaag ataagtggct
     3841 ggacataaaa atgtgcaggg ggagttcaga ggcaaaatac ctggggacgg gaaccctggc
     3901 ccggaaaaaa cgctcgcagt gtgaaggaca tttttatctt tataaacatt cacctaaagg
     3961 cagaaaaagc aggcgtgcca gagggaggcc gggcttacca acaacggaaa aagaacaaaa
     4021 agccgcaggc agagaaccat ggttaatatt cagcaataca gctgagttta atgccaaaaa
     4081 aatcatgaaa ctgtacagtc gaagaatgca gatagagcaa aactttcggg atgaaaaaag
     4141 tgaacgcttt ggttttggcc ttcgggcgag cagaagtcgg aggggagagc gttttctggt
     4201 attaagtcta ctggttacac tggccagtat cgtactctgg ctacttggtt atcattttga
     4261 aaataaagga tttcatttaa agtatcaggc taatagtctc aaaaaacgaa gagtattgtc
     4321 ttttctgacg ctggctgaaa acgtcctgag atttaaccct gaacttctac gacgagcaca
     4381 acctgaaaag atattgtgcc ggttggccag cacttaccga agtatggtgc tggcttactg
     4441 atgaaaattt tctggggatc cctcagcctt tcggggagga atgagggctc tgatattctt
     4501 ttgccgtagt tcatcgtgaa accgtcggat gtcatttggc ctgagtgacc catgcctccc
     4561 aaaagcagag tcgctagtgt tatgtaatta ggcggttaaa cttcaattct cagtgttaag
     4621 tttgggtaaa cgcttgtaaa tggtattgct aattttttaa ttgactaata ttagaaacgg
     4681 ttagtgtgat tgtatataga tatttgagtg taaggtattt aaaggggcat tcagcccctc
     4741 cgtctatttg tgattgcatc cattgatgtg tgtattattt tgagcttgaa aaagggaggc
     4801 gctctttaat aataaataaa tctatcaaag ctaaatatta aatggaagcc accctttttt
     4861 ctctgatagt tttattatca ccaaaccatt tatttcagct atcgaatctg gattcggcca
     4921 ggaaggtaaa ctcttcacaa ttggataaat cttctctatt tgttctttat ttgctggttt
     4981 gcaattacag attccatagt aattcataaa tgcaaccatc ctaaaatagt tgccaccatc
     5041 ccaccaaaaa aaagattttc caaaagtgtc tgcgctcatg gagagcatat ttcctgagtc
     5101 gtttgaacca tatatatata tatattttcc gttaagggaa atgccttttg tttgagatgt
     5161 gtatgatatt tctttagcta aagagatatc tttctgtctt gcgagagtat cattcaaaaa
     5221 tagatttttg gatgttgaaa cgccattaaa tataataaat aaaaaaaaca tgcagtttag
     5281 gtattttata gagcgaaaat tgcttaagca agaaaacaaa attactgcta ctataggcat
     5341 taaaacaaac aggcgaggtg gcgcccctga gccaacaaca acgataaata taaccggaag
     5401 tatataaatg aaaatgattg atgaaatcaa ataaattgat cttgttttta atttatatgt
     5461 tagggtaaac atcagaatta ataaaggaat cagtaaccac ttgtataggt ttaaaccagt
     5521 gggaggattg ttatagaagt tatatatatt atctatggct gatttaacaa gccatttaat
     5581 atcgcttgca ttttgtatat aatttgagat gtactcgttc gattcaagcg aaaaataatg
     5641 cttgatagct ttggttaata gcaggtaaat taaggtagat aaagctatga gggatagaga
     5701 taaacatgaa ctataaaaag attcacgaat atttttctca tttcgtatta tcgaatttat
     5761 ctgcaaccca atgacgaatg caataatata tgttacgaat gtttggtaga ttgccattga
     5821 aagaatggac agtactatac cagaaaatat cacaatccta tttttttgcg agtgaaaaat
     5881 aattgctgat atcgctgcca gtagaaaagc aattcccaca gtatcagctt ggttaagaaa
     5941 ctctaattga taggagatct gagggaaggt aataaatact aacataccta tcaataattc
     6001 ataagattct agctttagcg atctgcagat tataaatgct gaaatgatga taaaagataa
     6061 ggctattaat ggtgttatat aaagtgaaaa aggctcaggg agaaagtaat gtcgcaaaaa
     6121 agtgtgaaac caccgcccaa gcgtgattgt ttgataaaaa ttatttgtaa actcgccatc
     6181 aatattaagt gttccatcgt acaaccaccc ccaataacaa taaattgata ttgcaaaaca
     6241 tgatatgaag gcaaggagat ttgcattttt aaataaatta attttaatca tgggttacct
     6301 cgatgagatt tttttatgat gtatttgggt ctatttttta cttcaatata gattctacct
     6361 atatattctc cgagaacacc aatcccgatc agttgcactc cacccaagaa aagtattgat
     6421 acaagcaggg agggataccc gcgtactggg ttaccaaaga caagggtgtc tataatcatc
     6481 catgcaccat ataaaaatga aatgcttgca acaaacaagc ctatataagt ccatacgcga
     6541 agaggaaagg ttgaaaaact tgtgatacct tccagtgcca gattccataa tttccagcca
     6601 ttaaattttg agatgccagc aacacgctct gcgcgtacat attcaacgac atccgtcgga
     6661 ccacccaccc agctcagtat gcctttcatg aaaagattgc gctcaggcaa cagtttaatg
     6721 ttctccacaa cctcacgaga catgagtcga aaatctccga cattttcctc gatctttggg
     6781 gtgcttattt tgttgtgtaa tttatagaac cactcagctg tcttacgttt cagtcgtcca
     6841 tcagttgagc ggtctgaacg tttagcaagc accatgtcag cacctgcctg ccacttttca
     6901 ataagatgag ggataacctc aattgggtct tgcaggtcga catcaatagg aattacagca
     6961 tcgccgcttg catggtctaa ccctgcaaat aaggctggtt ctttaccaaa gttgcgtgta
     7021 aatgacagcg gaacaactag cgggtctgaa acagccagcg cgttaataat tgactctgtg
     7081 gcatctttac ttccgtcatt tatgaataca atttctactt catatggctt caactcttgg
     7141 aattcacgta ccgttttata gaaaacaggt atcgcttctt cttcattgaa gacaggaacg
     7201 acaagagata ttttcatttc gcatccctaa agacaatgaa ctttgaatag acgaaaccgc
     7261 acaccagact gatggcggag aaggtgataa gagtgactat tggaggaagt gagcatttat
     7321 cagctgccca accaacaatc acactcaata ttcccataaa tcccacgtat aacatgtagc
     7381 gcatcgctgt agtcaatgct ttgaatgtga atcttgcatt cgcgaagaag ctaaagctca
     7441 cagccactac gaaacctgtg aagtttgcca gagcctgact ggtatgtgcg gcatagatac
     7501 atacaccaaa aaccacccag tgtataagtg tgttcagcac acctatagat gtgtacttta
     7561 caaataactt taacatttat ttaatcaatg agctctgaaa ggcatgaagt ctatcatcca
     7621 agtctcaatt gatcgatact tgctatgtct gatgagacaa aactgagaca cataaggcct
     7681 cacaatggct tgcaaggctt tacatgtttt gatgtggtgg gacgtgtgag cgcagtgttg
     7741 atgggataat cctttgaatt acaagcggat tcttataatt cgtaatgcga aggtcgtagg
     7801 ttcgactcct attatcggca ccatttaaat caataagtta cctcgcattt aagtaaacta
     7861 cgttctcctc ttgtgccgta tttgtgccat tgcgacttat aatcgcatcg attttgctcg
     7921 cgtgctcggt aagatgcccg gctgaaaggt gggcgtatct ttgaaccatt tcgagagttt
//
LOCUS       DAJTNP010000152         7711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTNP010000152 DAJTNP010000000
VERSION     DAJTNP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..820)
                     /locus_tag="OO160_003755"
     CDS             complement(<1..820)
                     /locus_tag="OO160_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5525710.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRR"
     gene            1002..2426
                     /gene="sbcB"
                     /locus_tag="OO160_003756"
     CDS             1002..2426
                     /gene="sbcB"
                     /locus_tag="OO160_003756"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5525711.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2469..2696)
                     /locus_tag="OO160_003757"
     CDS             complement(2469..2696)
                     /locus_tag="OO160_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5525712.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2710..3768)
                     /gene="yeeE"
                     /locus_tag="OO160_003758"
     CDS             complement(2710..3768)
                     /gene="yeeE"
                     /locus_tag="OO160_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5525713.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3947..5305)
                     /gene="plaP"
                     /locus_tag="OO160_003759"
     CDS             complement(3947..5305)
                     /gene="plaP"
                     /locus_tag="OO160_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5525714.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5295..5357)
                     /gene="yoeI"
                     /locus_tag="OO160_003760"
     CDS             complement(5295..5357)
                     /gene="yoeI"
                     /locus_tag="OO160_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5525715.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5518..6501)
                     /locus_tag="OO160_003761"
     CDS             complement(5518..6501)
                     /locus_tag="OO160_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5525716.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6547..7371)
                     /locus_tag="OO160_003762"
     CDS             complement(6547..7371)
                     /locus_tag="OO160_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5525717.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7454..7708)
                     /gene="yoeB"
                     /locus_tag="OO160_003763"
     CDS             complement(7454..7708)
                     /gene="yoeB"
                     /locus_tag="OO160_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5525718.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2065 a         2012 c         1808 g         1826 t
ORIGIN      
        1 gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat acgttgtcca
       61 gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc ccccacagca
      121 taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca atagcaagcc
      181 aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg cgggagccga
      241 agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc cgcatgcacc
      301 aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga ttatcttttt
      361 cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc ggacgattag
      421 ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg gtcttttctc
      481 tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg accgtcatgg
      541 agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa tagagctttc
      601 gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat cgaccaagcc
      661 caagtcgatt aggcttgcca tgccagcgat agcggtactg gaactggatg acccccttcg
      721 gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc gaatatggtt
      781 taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt tggtacgctc
      841 agaaataata gtttggcaca catctggtac acaatatcac atggacaaca gcgcacagta
      901 aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc ttttcgagtt
      961 ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac acagataaac
     1021 aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc
     1081 ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag
     1141 tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta ttaattaccg
     1201 gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta
     1261 ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg
     1321 acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct
     1381 ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc
     1441 gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc
     1501 atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt
     1561 acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg tttgattatc
     1621 tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac
     1681 ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc tgggtggcac
     1741 cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg gcaggagaca
     1801 tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat accgcaaaag
     1861 ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg
     1921 tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc
     1981 agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg
     2041 tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat gcacagcttt
     2101 ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc
     2161 cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa aagttgttgt
     2221 tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc
     2281 tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct gatgaattgc
     2341 agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta aaagcacttt
     2401 ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg gcggaggaca
     2461 atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa
     2521 tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg
     2581 caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct
     2641 tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt
     2701 acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata
     2761 cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg
     2821 ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact
     2881 ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc
     2941 tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca cgaagatccc
     3001 taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgaag
     3061 aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg cttcactcag
     3121 gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg
     3181 tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag
     3241 ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggtaac
     3301 caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg
     3361 ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat
     3421 ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc
     3481 ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac ctataacagt
     3541 accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa
     3601 aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc gattattttt
     3661 cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa
     3721 tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac
     3781 cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca ataaccaaat
     3841 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc
     3901 ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt
     3961 cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa atcagaccga
     4021 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
     4081 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
     4141 tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca aaggtgaacg
     4201 ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag ttgattgcca
     4261 gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta
     4321 cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg
     4381 ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
     4441 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
     4501 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
     4561 agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
     4621 ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga
     4681 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgcgtgc
     4741 tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
     4801 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct acggatttca
     4861 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
     4921 gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
     4981 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
     5041 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
     5101 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
     5161 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
     5221 ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
     5281 tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac cgtcgcgtaa
     5341 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
     5401 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
     5461 aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc attgtgccta
     5521 aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc tttgaatggc
     5581 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
     5641 atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa tccagacagg
     5701 ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg
     5761 ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca gctccagttt
     5821 ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga
     5881 agcgcctgcc tgcgcagtat cccccaccac aatcgcccga tatcgtttaa tgatacgacg
     5941 gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
     6001 aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
     6061 aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga gtacgccatt
     6121 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga gaggcgcaag
     6181 cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
     6241 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc
     6301 taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat caagcaattg
     6361 aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
     6421 gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga ggatcatcaa
     6481 cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt
     6541 gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
     6601 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
     6661 ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg
     6721 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
     6781 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc
     6841 ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc ccgcaagacg
     6901 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
     6961 tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc cttgcgcgtc
     7021 gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg ccagcgcgct
     7081 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
     7141 cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt cagaatcgca
     7201 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
     7261 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
     7321 cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca tccgttatct
     7381 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
     7441 tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcatcagtaa
     7501 ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct gacaaattat
     7561 gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta tctttgataa
     7621 gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc
     7681 atgattcctc agaccagatt agtttcactc a
//
LOCUS       DAJTNP010000153         7633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTNP010000153 DAJTNP010000000
VERSION     DAJTNP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..213
                     /locus_tag="OO160_003764"
                     /pseudo
     CDS             1..213
                     /locus_tag="OO160_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            886..2394
                     /gene="ltrA"
                     /locus_tag="OO160_003765"
     CDS             886..2394
                     /gene="ltrA"
                     /locus_tag="OO160_003765"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5525719.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2484..2957
                     /locus_tag="OO160_003766"
                     /pseudo
     CDS             2484..2957
                     /locus_tag="OO160_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            3122..7240
                     /gene="pic"
                     /locus_tag="OO160_003767"
     CDS             3122..7240
                     /gene="pic"
                     /locus_tag="OO160_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5525720.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(7391..>7633)
                     /locus_tag="OO160_003768"
     CDS             complement(7391..>7633)
                     /locus_tag="OO160_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR5525721.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1999 a         1786 c         2113 g         1735 t
ORIGIN      
        1 cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg tggccagatg tacagtggcg
       61 cgcctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt ccgtacgacc
      121 atcagccgga aagcctttgc cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga
      181 cctgaccagc tgtgggtggc tgattttact tacggtgtgc ccggagttca gggcgggcat
      241 ggatgcttaa atgaaccgcg agtctgtctg gaatattgaa ccggtaactc acgatgagaa
      301 acccaacaat cccaccgggt gtgacggtgg agaacctgag cggcagtgac ctgcggcatg
      361 cccgcagggt gatgtaaccc gctgacaacg gggattgagg cgagatcact aagccgagat
      421 gatcctcaag gttaagtact gaaaggctga agaacatgaa cccgttaatc cgcctctgtg
      481 ggttgaaaac gtcaccacgg cctacgtgat ctgacaggcc gtgcaggagg aactggcagt
      541 gatacgtaag cactgccggt cgaaggtgtt ttgacatgta tgcgaaacac cggggcagca
      601 gcgtctatca cacttgcgtt gctgacttct gccaacttgc ggcaagcaag gataaagagt
      661 gcgacgggca gcctcctcag tatgcctgag tccaggcagg taaaccgggg aaggtcagcg
      721 acggatgtta agggggcatg gctccgatga cgcgctggct ggcggagctt ccgtagtagt
      781 ccgcgatggg gaaagcccat tacatggcga agggaagcag tttgaatgtg tttgcgacgt
      841 gaattaactg acctaacgag gtgaagacct ttgataatca gcgaaatgca acgcaagctt
      901 gccacatggg cagccaccga tccgtcccta cggattcaac ggctgctgcg tctgataaca
      961 caaccagaat ggctggctga agcggcgcgg atcacgcttt catcaaaggg ggcccatacc
     1021 cccggcgttg atggcgtgaa caaaacaatg ctacaggcca gactggctgt tgagctgcaa
     1081 atcctcaggg atgaattact ctcaggccac taccagccct tgcccgccag acgggtttac
     1141 atccctaaaa gcaacggcaa actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt
     1201 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
     1261 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
     1321 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
     1381 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
     1441 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
     1501 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
     1561 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
     1621 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
     1681 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
     1741 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
     1801 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
     1861 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
     1921 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
     1981 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
     2041 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
     2101 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
     2161 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     2221 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt accggttggt ggggcaaggc
     2281 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     2341 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     2401 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     2461 actacgccct gcctcttact ctactcagca catggcaggg cttcgtctat gtggcgttca
     2521 tcattgatgt gtttgccgga tacatcgtgt ggtgacgagt ctcatcgtct atggaaacga
     2581 cattcgtgct ggatgcactg gagcaggcgt tgtgggcccg tcgtccgtct ggcaccatcc
     2641 atcacagcga taaaggctct cagtatgtgt cactggccta taaggcgcga ctaaaagaag
     2701 caaaactgct ggcatcaaca gggagtactg gtgactcgta tgacaacgcg atggcagaga
     2761 tcatcaaggg cctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccggacag
     2821 aagtggagct ggccacactc acgtgggtgg actggtataa caatcgacga ttgctggaaa
     2881 ggctgggcca tatcccaccg gcagaagcag aaaaagctta ttatgcttcc atcggaaatg
     2941 atgatctggc agcctgagtt cacagataaa acactctcca ggaaacccgg ggcggttcag
     3001 ttcacaaaaa cacattaatg cagtaactat attttccttt ctggtgataa cgtcgggtta
     3061 tcattagctt cttcagctat tttactttta tatcccttgt aaacatcatg gagaatccat
     3121 agtgaataaa gtttattctc ttaaatattg ccccgtcacc ggggggctta ttgctgtctc
     3181 tgaacttgcc cgcagggtaa taaaaaagac atgccgaaga ttaacgcata ttcttctggc
     3241 tggcattcca gcaatctgtc tgtgttactc tcagatatcc caggcgggta ttgtccgttc
     3301 cgatattgcc tatcagattt atcgtgattt cgccgaaaac aaagggcttt ttgtacctgg
     3361 tgccaatgat attccggtat atgataagga cggaaaactt gtgggaagac tgggtaaagc
     3421 cccaatggcc gatttcagca gtgtgagctc aaatggcgtt gctacgcttg tatcgcctca
     3481 gtatatcgtc agcgtaaagc ataacggagg atatcggagt gtgagctttg gtaatgggaa
     3541 aaatacatat tcccttgttg accgtaataa ccacccttct attgacttcc atgctccacg
     3601 tctgaataaa ctggttacag aagttattcc ctcagcggta acatcagaag gaaccaaagc
     3661 caatgcttat aaatacactg aacgttacac cgctttttat cgggtgggta gtggtacgca
     3721 gtacactaag gacaaggacg gaaatttagt taaggttgcc ggtggatatg cttttaaaac
     3781 aggaggaacc acaggagttc ctctgatatc tgatgcaaca atagtctcta atcccgggca
     3841 aacttataat cctgtaaacg gccctttacc tgactatgga gcccctgggg atagtggttc
     3901 tcctttgttt gcttatgata aacaacaaaa aaaatgggtt attgttgctg tattaagagc
     3961 atatgcaggt attaatggtg ctacgaactg gtggaatgtc ataccaacag attatctgaa
     4021 ccaggttatg caggacgatt tcgatgcccc cgtagacttt gtttccggac tgggccccct
     4081 gaactggaca tacgacaaaa catcaggcac aggtaccctg agccagggca gtaaaaactg
     4141 gaccatgcac gggcagaaag acaatgacct caatgccggt aaaaatctgg tattcagcgg
     4201 gcagaatggt gcaattatcc tgaaagacag tgtgactcag ggtgccggtt atctcgaatt
     4261 taaagacagt tacaccgtat ctgctgaatc cggaaaaaca tggacgggtg ccggcattat
     4321 tactgacaag gggacgaatg taacctggaa ggtcaacggc gttgccggtg acaacttgca
     4381 taagctgggg gaaggaaccc tgaccataaa cggaacaggt gtaaacccgg gaggactgaa
     4441 aacgggagac ggtatcgttg tacttaacca gcaggcagac actgcaggta atatccaggc
     4501 cttcagttca gtgaacctcg ccagcggacg tccgaccgtg gtgctcgggg atgcccgtca
     4561 ggtcaatccg gataacattt catggggata ccggggaggt aagcttgacc ttaatggtaa
     4621 tgccgttacc ttcacccgac tgcaggctgc tgattacggg gcggtgatta caaataatgc
     4681 acagcaaaaa tcccagcttt tactggatct taaggctcag gatacaaatg tcagtgaacc
     4741 gacgattgga aatatatccc cctttggtgg taccggcact ccaggaaacc tgtacagcat
     4801 gatactcaac agccagaccc gcttctatat tctgaaatct gccagctatg gtaacactct
     4861 gtgggggaac agcctgaatg atccggctca gtgggagttt gttggcatga acaaaaacaa
     4921 agcagttcag acagtaaaag ataggatcct ggccgggcgg gcaaaacaac ccgttatctt
     4981 tcatggtcag ctgaccggga atatggatgt cgccattcca caggtgccgg ggggaagaaa
     5041 ggtaatcttt gatggtagcg tgaacctgcc ggaaggtacc ctgagtcagg acagtggcac
     5101 cctgatattc cagggacatc cggttatcca tgcctccatc agtggcagtg caccggtcag
     5161 cctgaaccag aaagactggg aaaaccgtca gtttacaatg aaaacactgt cgctgaaaga
     5221 cgctgacttc catctttcac gtaacgcctc gctgaacagt gacattaagt cggataacag
     5281 ccatatcaca ctgggaagtg acagggcatt tgtggataaa aatgacggaa caggaaatta
     5341 tgtcattccg gaggaaggta cctctgtccc ggacaccgtg aatgacagga gccagtatga
     5401 agggaatatt acgctgaacc ataactcagc cctggatatc ggcagcaggt tcaccggggg
     5461 gattgacgct tatgacagtg ccgtcagcat cacctctccg gacgtcctgt tgacagcccc
     5521 gggtgctttt gccggcagtt cactgacagt gcatgatggc ggtcatctta cagcactgaa
     5581 cggtcttttc agcgacgggc atattcaggc cggtaagaac ggcaaaatca ccctgagcgg
     5641 tacaccggtt aaagatacgg ctaatcagta tgcccctgct gtatatctga cggacggata
     5701 tgacctgacc ggcgataacg cagcactgga aattacccgt ggagcacatg cttccggtga
     5761 tattcatgcc tctgcggcat caacagttac catcgggtct gacacgccgg cagaactggc
     5821 ttctgcggaa acggctgcat cggcgtttgc cggcagtctt cttgagggct ataacgcagc
     5881 attcaatggt gccataaccg gtggcagggc tgatgtcagt atgcataatg cactgtggac
     5941 tctgggtggg gactctgcca tccacagtct taccgtcaga aacagccgta ttagttctga
     6001 aggagaccgt acattccgta ccctgacggt gaataaactg gatgcaacag gcagtgattt
     6061 tgttttgcgt acggacctga aaaatgccga taaaattaat gtgactgaaa aagccactgg
     6121 ttcagataac agcctgaacg tcagctttat gaataatcct gctcagggac aggccctgaa
     6181 tattcctctg gtcacggcac cggcgggaac ttcagcagag atgtttaagg ccggcacccg
     6241 ggtgacaggt ttcagtcggg tgaccccaac cctgcatgtt gataccagtg gtggcaatac
     6301 gaagtggata ctggatggtt ttaaagcgga ggctgataaa gccgctgccg cgaaggctga
     6361 cagtttcatg aatgccgggt ataaaaactt catgacggaa gttaacaatc tgaacaaacg
     6421 tatgggtgac ctgcgtgaca caaacggtga tgccggtgcc tgggcgcgca tcatgagtgg
     6481 tgccggttct gcagacggtg gttacagtga taattacacc catgttcagg tcggctttga
     6541 caaaaaacat gaactggacg gtgtggacct gtttaccggt gtcacgatga cctataccga
     6601 cagcagtgca gacagccatg cattcagcgg aaagacgaaa tcggtggggg gcggtctgta
     6661 tgcttcagca ttgtttgagt ccggtgccta tatcgatttg attggtaaat atattcacca
     6721 tgacaatgat tacacaggta actttgctag cctgggaacg aaacactaca acacccattc
     6781 ctggtatgcc ggtgctgaaa cgggttaccg ctatcacctg acagaggaca cgttcattga
     6841 gccgcaggct gaactggttt acggcgccgt gtccgggaaa acattccgct ggaaagacgg
     6901 tgatatggac ctgagcatga agaacaggga cttcagtccg ctggttggaa gaacaggggt
     6961 tgaactgggc aagaccttca gtggtaagga ctggagtgtg acggcccgtg ccggaaccag
     7021 ctggcagttt gacctgctga ataatggaga gaccgtactg cgtgatgcgt ccggggagaa
     7081 acggataaaa ggagagaagg acagccggat gctgtttaat gttggtatga atgcgcagat
     7141 aaaggacaat atgcgctttg gtctggagtt tgagaagtca gcctttggta aatataacgt
     7201 ggataatgcg gtaaacgcga atttccggta tatgttctga tgtgggggta aagggctgcc
     7261 ggaaggcagc ctttctcaga gactcttaaa atataaaact gaaggtcttc ggtaaggggg
     7321 agtgtattag acgtcaacta gttttgccga ctggcgcgag cctgtttaag attctgtgta
     7381 aatgcctttc tcagaagtga ccgtccaggc ggtcaccgaa ctcgataata aagcggctca
     7441 ttgccattcg ccagtccttc aacggcatcg tccatttctg ggacgcagac tggattgcca
     7501 gccacaccac ttttttcacc gagtcgtctg tcgggaacac tttacgcttt ttgatcgcat
     7561 ggcggatcac gctgtttagc gactcgatgg cattcgtcgt atagatcact ttgcggatgt
     7621 ccgttggata agc
//
LOCUS       DAJTNP010000154         7536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTNP010000154 DAJTNP010000000
VERSION     DAJTNP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO160_003769"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO160_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO160_003770"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO160_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5525722.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO160_003771"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO160_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO160_003772"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO160_003772"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5525723.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO160_003773"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO160_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5525724.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO160_003774"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO160_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5525725.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO160_003775"
     CDS             complement(6510..7202)
                     /locus_tag="OO160_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5525726.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1827 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccccgcgccg ctgagaaaaa gcgaag
//
LOCUS       DAJTNP010000155         7364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTNP010000155 DAJTNP010000000
VERSION     DAJTNP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            41..1531
                     /locus_tag="OO160_003776"
     CDS             41..1531
                     /locus_tag="OO160_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5525727.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1583..2740
                     /locus_tag="OO160_003777"
     CDS             1583..2740
                     /locus_tag="OO160_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5525728.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2706..2828)
                     /locus_tag="OO160_003778"
     CDS             complement(2706..2828)
                     /locus_tag="OO160_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525729.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3031..4716
                     /locus_tag="OO160_003779"
                     /pseudo
     CDS             3031..4716
                     /locus_tag="OO160_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4754..7114
                     /locus_tag="OO160_003780"
     CDS             4754..7114
                     /locus_tag="OO160_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5525730.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7171..>7364
                     /locus_tag="OO160_003781"
     CDS             7171..>7364
                     /locus_tag="OO160_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525731.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
BASE COUNT         2161 a         1611 c         1564 g         2028 t
ORIGIN      
        1 atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc ttggttatgg
       61 acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc gtgaagttgt
      121 cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat caacgccgac
      181 gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg cacacggtgt
      241 ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct ttggtgtcta
      301 ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc ctgaattatt
      361 ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa aaatcagtaa
      421 tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca ccacattttc
      481 tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat tccacgctgc
      541 aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa
      601 aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc gtgccaaaac
      661 acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac caaatgataa
      721 tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag cagataacta
      781 ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa
      841 aaacggacag tatgacaata caattattct ctttacctcc gataatggtg cggttatcga
      901 tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct atcctggcgg
      961 tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta attatgacaa
     1021 gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata tcagcattcc
     1081 aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata agaaacaagg
     1141 cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg atgaggaaaa
     1201 tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg attacccgca
     1261 taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata acgattattc
     1321 gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg atctacagca
     1381 aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag
     1441 agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga agtttaacaa
     1501 tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg atttttccgc
     1561 cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa
     1621 tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc atgaaaaatg
     1681 gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt ctggcaatca
     1741 ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt ttttccgtaa
     1801 agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata cattacaaac
     1861 gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg aatttctggt
     1921 tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca gtaattcagg
     1981 taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat atcaagtaga
     2041 gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg aggtttatca
     2101 ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag aaaccgggac
     2161 gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg atttttctgt
     2221 gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg ttaaaaacga
     2281 tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt tggggaatgg
     2341 gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg aaagtaatgg
     2401 agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa acattaataa
     2461 atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac ggatttcagc
     2521 gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta agcatcgtat
     2581 tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa tccttttttc
     2641 aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag taccgcttta
     2701 ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc ttttaactcc
     2761 attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc tgtcgggtgt
     2821 ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg tcagggcatt
     2881 agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa aatttctata
     2941 tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt gccatagata
     3001 atcattggat ggcacaatca attttcgcta atgataacca ttcccattac gttttgtatg
     3061 ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact
     3121 tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa aattcaggtc
     3181 tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag
     3241 ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt aatatccgca
     3301 aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac
     3361 taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat
     3421 aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcaacaaa
     3481 acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt
     3541 attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc
     3601 caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt atttacccat
     3661 aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt
     3721 cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag caatgctgag
     3781 tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt
     3841 ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc
     3901 gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga
     3961 ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg gtttatttat
     4021 aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga gttccatcaa
     4081 ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt acaagtggct
     4141 aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa ctttcatgtt
     4201 tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac cacactgctt
     4261 aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc tgctgacagt
     4321 tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat tatttgtaaa
     4381 gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca ggttggtctg
     4441 ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct ttccagcggc
     4501 gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg gatatttctt
     4561 gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg tttagtgcgt
     4621 gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt ctggaacctg
     4681 gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc ctgctgtacg
     4741 gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg gcgctgatgt
     4801 ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga tgacggtcta
     4861 tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa gcatcgagcg
     4921 tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg cggtacgcat
     4981 ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga aaatctctat
     5041 tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg caactaataa
     5101 cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg ggatgtcaca
     5161 ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca gttttgtgcc
     5221 ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac gcttcaacgc
     5281 tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt taacatcgca
     5341 tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta cctatttctc
     5401 tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg ctgataactt
     5461 tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg attatgtttc
     5521 gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata ctgcattgag
     5581 caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa aatataccgg
     5641 ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa
     5701 atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac tacgtaccac
     5761 cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct ggtacaccga
     5821 actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat taggacacat
     5881 ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa aattcgccgt
     5941 gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg atgcatggac
     6001 tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga aaactaacca
     6061 taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc tgtatatggc
     6121 ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg actatgacaa
     6181 ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata tttttgctga
     6241 tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata ttcttgatat
     6301 gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata aaaccctgac
     6361 gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat tgcagcaaaa
     6421 aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca gcaaaagcag
     6481 tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg gcaaaaccaa
     6541 aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc gccaggtaga
     6601 tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa
     6661 tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta atctggtctc
     6721 ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct taaacatgga
     6781 tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg
     6841 taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat attcagatta
     6901 caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc gcttgtcctg
     6961 gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc tcaatgtact
     7021 ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg ccagtggccg
     7081 tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact taaccgggta
     7141 aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt actgacgtta
     7201 gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca ggatgaaaag
     7261 ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca tgctcatcca
     7321 aaggatcagg taaatttatg gctgcaaatt cataccggtt catt
//
LOCUS       DAJTNP010000156         7192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTNP010000156 DAJTNP010000000
VERSION     DAJTNP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..410
                     /locus_tag="OO160_003782"
     CDS             <1..410
                     /locus_tag="OO160_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5525732.1"
                     /translation="LVEQIAELESELSQSINADDTAQRIMTIPGVGPITASLLSSQLG
                     DGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLER
                     QHGKLAEWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(749..1519)
                     /locus_tag="OO160_003783"
     CDS             complement(749..1519)
                     /locus_tag="OO160_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525733.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1564..1998)
                     /locus_tag="OO160_003784"
                     /pseudo
     CDS             complement(1564..1998)
                     /locus_tag="OO160_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2010..2231)
                     /locus_tag="OO160_003785"
     CDS             complement(2010..2231)
                     /locus_tag="OO160_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525734.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2232..2915)
                     /locus_tag="OO160_003786"
     CDS             complement(2232..2915)
                     /locus_tag="OO160_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5525735.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2979..3290)
                     /locus_tag="OO160_003787"
     CDS             complement(2979..3290)
                     /locus_tag="OO160_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525736.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3287..4189)
                     /locus_tag="OO160_003788"
     CDS             complement(3287..4189)
                     /locus_tag="OO160_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5525737.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4629..5588
                     /locus_tag="OO160_003789"
     CDS             4629..5588
                     /locus_tag="OO160_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5525738.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5588..5983
                     /locus_tag="OO160_003790"
     CDS             5588..5983
                     /locus_tag="OO160_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5525739.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6166..6546
                     /locus_tag="OO160_003791"
     CDS             6166..6546
                     /locus_tag="OO160_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5525740.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6592..7192
                     /locus_tag="OO160_003792"
                     /pseudo
     CDS             6592..7192
                     /locus_tag="OO160_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1945 a         1864 c         1759 g         1624 t
ORIGIN      
        1 accttgttga gcaaatagca gagctggaat cagaattaag tcagtcaatc aacgctgatg
       61 ataccgctca gcgcattatg acaattcctg gtgttgggcc gattactgcc agcttactat
      121 cgtcgcaact tggtgacggg aaacagtttt catgcagcag agattttgcg gcttccacag
      181 ggctcgttcc ccggcagtat agcactggag gaaaatcgac tctgttgggt atcagtaagc
      241 gaggtgataa aaatttacgc cggttactgg ttcagtgtgc ccgctcattc atgatgcagc
      301 ttgaacgcca gcatggaaaa cttgctgagt gggttcggga gcaactcaac aagaagcatt
      361 cgaatgtggt tgcctgcgct ctggcaaata aactggcccg aattgcctag gcaatcacta
      421 ctcagcaaaa agaatatcag gcctgatata tagatgctga caaaactcaa ttagcttatt
      481 gcaaacaata agatacaagt taccgtctgg ttttgcgaag attgattatt gatgacatga
      541 acggcaaatc gacctgatta aaaacctaac aggggaaaat ggctcactga agccgagggg
      601 atttttagga ttatcaggtg cggaactcat cgaggcgcgg gcatcccttg cccacagaga
      661 cgccggatag atttaagcaa gcccaccatt aatcaaaaat cagtgttgca aaaacggggg
      721 tgaccataga ttttccccgc catcgtcatc atttccggat ttttgccagg gcatccgcca
      781 tgctgcggat ttcggcccgg gcttcccgga caacttcccg cagaacacca cggtcaaacc
      841 accggcgggg cggttcatcc ggtatacgca gcacatcgca ggccagccca ctggacagga
      901 tatgctcaat acaatcgctg accagataca tttccaccgc cgaaaaacgt gcgccccggt
      961 tcaggatggc atcgagcacc acgggcaggt tttcatcgtc ttcatcgagc cagcaggggt
     1021 gatcgctttc cttcacgacc aggcgaaagc ggcgcgtctg gcgggtcagc aacgtctgac
     1081 cgcttgtggt cagcgccgcc cggatacgcc gcgcatcttc tgccccccgg caactgacac
     1141 tgcatactgc atcgcgcacc gcatcaagaa ctgccctgat ttcgtcttcg gtcagccatt
     1201 caccggcata cgggaaagac acctgcgttc cgtccgccgg aagtaccggc agcgactgcc
     1261 actgactgcc ggacagaaca agttccgctc cggttccggt cagtttctga atggcggagg
     1321 ccaggtgcgg cggcagcacg ccatcgtggc ggttaacgag taacgtcgcc agccggggcg
     1381 catccagccg cacgtaaatg ctgatccagc ccggatagtt cgggttacat tggtcaacat
     1441 ccgtctcttc cggccagccc tgcgccagca gcaggttacg ggcgtgacga caaatcagag
     1501 cattcagcgt ttcattcata attcatgaac tccctgtatc gccgcccaca ggcggcgata
     1561 acatcagtcg ttttcgtgtg tgttgttttt cagcaggctg cgggcctgat caaccatcgc
     1621 ctgtcggcgg gtaacgtatt ccgggagggg gaaaccgtga tcccgggcag cccattcacg
     1681 tttccacagt gcctgttcag cgatctgagt cagggtctcc agcagttcag cagggacggt
     1741 cacctggcgg ttttcgcgca cgttgctgat aatctccatc gccgcggcgg aagaaatacc
     1801 ggactcaatc cgctagtctt ccgggatgtc ctccaggtgc gccaggacgg tcctgatttc
     1861 atgatcggac agggaaatat ccattccgtc agcgagggcc tgaatctctt ccggggacca
     1921 gataaccagc atcagcggcg tgtctcccga atactttgct gccagcgccc ggcatagcgt
     1981 ttcgcatgtt ccgtacatac tctctcccct caatctttga agcaggaatc cagttccaga
     2041 atttcggtat atgcctccat caagaggcgg tttgcgcgaa acaacgtctg acgcaccaga
     2101 cgttccggca gggtgtcagc attaaagctg taccgccgct ccatctcatt catggttgta
     2161 cgcaggtcgc acatggcatt ggccacctcc gccacttcgg cgcgaagttt ttcgtgtccg
     2221 gcatagttca tttacgcagc ctccggcata aaccagtcat cattcgcggc cccctgctgc
     2281 atggcccgct gtacggcagc cagtcgttgc tgtccggcac ggtgatactg ctcaagcagc
     2341 tcgataccga tataccggcg tccggactgg agggcggcga cgcaggttga accgctgcct
     2401 gcaaacgggt ccagcactat cgcattcggg tgtgtgaagc tctcaatcag cggttgcagg
     2461 ctggtaacgg gcttttccgt cgggtgatgg cggttgcccg aatatttcca gcccaacacg
     2521 tccggcagcg ggttttgtgg cagacgtgga cggccttttg ccaggatgta ggcgcattcg
     2581 tggcgatagc ccacatatgc ggccttcgat gtgtagtttt tggtgaagac caggtgacca
     2641 acaacgctga atcccgcatt tttccaggca gacataaagc gatcgacgcg gttccagcca
     2701 tagaagctca ccatcagcgc gtcttttttg agtacgcggt acatttcatt acaggccggt
     2761 tgcagccatt catcagtttt atcaccagca atggtacgcc cctgacggtc acgaaaaccg
     2821 acgagatatg gcggatcggt gaggatgaaa tcaatggcgt tatcaggaat acgggccata
     2881 acatcgatgc agttaccgag gacaaaacgg gacataacag atccttctct tacagagggc
     2941 acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc atcgcttccg gtaacgggtc
     3001 aggccgaccg tggaataccg cagccgtcgt ctttttgaca gcaaggttat gccgcgaaag
     3061 cggcttgaag ggcgttcatc agaaaagggc gacgggctgg cgtacactgt caccgacacc
     3121 agccgggagc ggtaaaagcg cccgtcccgt gcccgccgga cgcgggacac tgaccacagg
     3181 cagcgtttat gccgcgccgc gtcagccggc gcagtaaaaa gaggagcacg gcgacatcct
     3241 ttgtcggaaa agaaacgggc ggttgcccgc ccgtctccgt catacttcat ccgccataat
     3301 gcgccacatt gtcgactatc cacgcaggtc cggtgacttc cggctgctca tcgtcatctg
     3361 tcggggaaga ccattcaagt tccccccaca gcacattgac gagctctcca cgctcataca
     3421 actgccagcc acagaaatca cagccctgtt cggcatacca gtgctccagc gtgcagctga
     3481 aacggcggct taattctgca ataacgtcgc tttccggctg acaccacgga gtgtcgaaat
     3541 ccacctgaat gaagttttct ccctgactgc tgatattcag gtcatatcca caaggccact
     3601 tcacgccata ccgttccgta taccagtgat atgcagaagg aacaccgttc agaacaccac
     3661 cattaaagcc gttcacttca acatccaggc gggtcggcag aaccgtcagc aggtcaaacg
     3721 gcatggtttt ttccggcact acgttgtcac acagacggtt ccaccacacc gatacatcct
     3781 cgttacccca tatgtcacac cagtcacctc tttttgcggt gaaatgcaca gtgatggttt
     3841 ccctggcatc atccggtaat ccttcccaac gacgctggcc agtaccgctc tgtaaccaga
     3901 gttcatgcag catcaggcag ttttgttcat ccagcaacac accgttctgc aggtgtgtca
     3961 gccagcgggt gaacgcgata ttttccgtcg atacagcacc acgtccggca gccgtcactc
     4021 cggggcactg ttcagagcgg atattttcag tgatctgcag aagtccggca cttccggcca
     4081 gaaacagctg aataccttca tttgtggcgc ggcgataaag cggtgtgact gcaccgctgg
     4141 ccagtcgttt aatctcagcg atctgtgccg gctcaccagg aaaatacata cgattcgagc
     4201 accagtttgg catgggaatt ctcctctcac atgcggcctc catcaggccg tgccgcacct
     4261 gtaaccgggc gaccgcatac gaatgcaggt gcggccccga agccgcagcg cgtggaggag
     4321 gaatgacgaa acgtgaccgg gtgaggaggc cggcgacagc gacacacttg catcggatgc
     4381 agcccggtta acgtgccggc acggcctggt taaatagtat ccgtctcagt tcgtggcgca
     4441 aaatgagaaa ccaataagaa ctcaaaatga accacaacaa gaacataaag agactctaaa
     4501 agagcaacac cgtttgacat agactcaaaa tgagtcacaa taagaaacaa taaaaaactc
     4561 aaaaagatgc accgacataa aggatgctca atttgagata aacacaaact caaaacgagg
     4621 caaaaagaat gctaaaagta tcatgcgatg atggttcaac taacgtaaaa ctggcctggc
     4681 ttgaagatgg cgaggtaaga accagtctgt caggaaacag ctttaaagaa ggctggaacc
     4741 cgggactgtt taatgccggc aaagtttaca attacgtcgt ggatgaaaaa aaatacacct
     4801 atgatttggg aagcactgct gtcataggta caacacatgt cagttatcaa tacagcacca
     4861 ccaatctgct tgccatacat catgcactgc tgacatccgg ccttcagcca caggatgtag
     4921 aactgacagt aacattacct gtgaccgaat tctttgataa tgacaatcaa ccaaatgaag
     4981 aaagaataga aagaaaaaag gcaaatgttc tccgtgagat atctctgaat aaaggggaaa
     5041 catttaaaat taaaaaagtt aatgtaatgc ctgaatcttt gccagctgca tttgaatcac
     5101 taaaaaaaga taaagtaaac aaacttgaac gcagcctgat tatcgatctt ggaggaacca
     5161 ctctggattg tggtttaatt ctcggtgcat ttgaaggcat ttctgaaatc cgtggctatt
     5221 cagaaatcgg gacatcccgc atcactcata ccgtcatgaa tgcgctgacg aaagcctcca
     5281 caccatgtaa ttattttatt gctgatgagt taataaaaaa cagacacgat aatgaatatc
     5341 tccagacatt aataaatgat gtagctgaaa taaaaaacat ttctcatgta atagacagag
     5401 aagtgaaatc tctggcagaa agcatcaggc aggaaatttc aacattctca ggaatgaacc
     5461 gaatctatct cactggcgga ggagccgagc ttatttaccc tcacataaag caatatttcc
     5521 cgaatctgaa agtgaacaag gtggatgaac ctcagtttgc attggtgaaa gcaatggtgc
     5581 atgcgtaatg gaaagcagtg atccaaaaaa aagaaagaaa gttgtggcat acctgcaccc
     5641 ggcactttat ccgcaggaca acctgacaca acaaacaata gactctctcc cagtacaaat
     5701 gcggggagat ttctacagac aatcccttat ttgcggagca gctctttatt ctgttgcccc
     5761 ccgactgtta acgctaatca gcgttttttt cagtgagaaa atcactgcag aaaatctggt
     5821 gaaacttata gaacaaacaa caggatacac atccacatca attgatataa gtgtgttaaa
     5881 aaacatcata gaggcatctt cagaaaacaa atcagagagc atcacttcaa aagatgattt
     5941 cgaagaacaa accagacgca atctttctat gttaaaaaaa taataaaaga agagcaggtt
     6001 cagtcaatta cctgctcttc ttttatacat actaaaaaat cagattttta cgaatattca
     6061 gattatcata aaaaatgcaa cagacaggaa tgcaataatt ttgcattatc tcgttgcttg
     6121 taatgcaaat atgcattatt gtcagtgtgt gacaataaca gagagatgac gatggcgacc
     6181 attaacgcac gtattgatga tgatataaag aaccaagcag atgaagtgct gaagctgatg
     6241 aatatcagtc aaactcaggc tattgcagct ttttatcagt atattactga acaaaagaaa
     6301 ctgccatttg tgatcacctc aattgtaaaa acgccgcatg atttattacg tgaaagtaca
     6361 gacatgttag ctgaagctct tgcagtgatt tccaatctac aggtgtggac tgaacagcag
     6421 gatgggattg gaaaagcaaa gctaatggag tattaccgcc gcctcgacgc actgtactgt
     6481 tgtgcaaaag agaaaatagg tcttctttca gataaccggg atgctgaact aggctgtgtc
     6541 ccttaatcat ctgagttata gtaactgtct gtttacacaa catattgaac tatggctcgc
     6601 tacgaccttc ccgatgaggc atggaccatc atcaagcccc tgttacctcc tgaacccgcc
     6661 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
     6721 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
     6781 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
     6841 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
     6901 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
     6961 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
     7021 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
     7081 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
     7141 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aa
//
LOCUS       DAJTNP010000157         6962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTNP010000157 DAJTNP010000000
VERSION     DAJTNP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(364..945)
                     /locus_tag="OO160_003793"
     CDS             complement(364..945)
                     /locus_tag="OO160_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525741.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(992..4849)
                     /gene="sigA"
                     /locus_tag="OO160_003794"
     CDS             complement(992..4849)
                     /gene="sigA"
                     /locus_tag="OO160_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5525742.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5210..6214)
                     /locus_tag="OO160_003795"
                     /pseudo
     CDS             complement(5210..6214)
                     /locus_tag="OO160_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6199..6576
                     /locus_tag="OO160_003796"
                     /pseudo
     CDS             6199..6576
                     /locus_tag="OO160_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6615..>6962)
                     /locus_tag="OO160_003797"
     CDS             complement(6615..>6962)
                     /locus_tag="OO160_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5525743.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1734 a         1567 c         1573 g         2088 t
ORIGIN      
        1 atcacctgct attaatcccc gagtcagtac ttcgcggcct gacgcgatca ccctactcag
       61 tacttcagag cccgcctccg gtacctgcct ggcgatcgcc cctgtgcaac aaaataaatc
      121 aaccgttatc aaaccacgat cgttcgtatg cgccgccgat gacgcgcgcc tgaaaaacgc
      181 gtcaatatga gttccccgga agcttacgat aatacgctta tgaacattac aacacgctca
      241 tgcggaacct ggatggggga aggaacggtg aagctgttag cgaatgaccg gcgtttatac
      301 aaacgccggt catgatgaat gatatttcag aatatacctc gggctaaaat accgggacag
      361 agatcaggcg tgattaatct gcagcatttt taataacaga agtactgagc ttactttacc
      421 tttgatgatg taatagttat ttccgtcggt actcattttt ccctgaaata atgtcacgga
      481 tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc atatttttaa tgagctgaga
      541 tccgggtggc aaggggtatt tgctgtcaga tactgggcga ctgagtctga tgtggcgcgt
      601 atacagcccg ttttcgtaca gggtggcctc tgttatggtg tcagtgacca gcatgtggga
      661 gacaatgctg tattcttttt taaagcaccc accagaaacg gtgattgtaa tgataatggt
      721 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      781 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      841 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      901 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctccggtaga
      961 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
     1021 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
     1081 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
     1141 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
     1201 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
     1261 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
     1321 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
     1381 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
     1441 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
     1501 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
     1561 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
     1621 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1681 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccaccagaag
     1741 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1801 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1861 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1921 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1981 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     2041 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     2101 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     2161 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     2221 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     2281 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     2341 ctgcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     2401 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     2461 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     2521 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     2581 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     2641 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2701 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2761 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2821 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2881 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2941 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     3001 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     3061 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     3121 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     3181 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     3241 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     3301 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     3361 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     3421 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     3481 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     3541 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     3601 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3661 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3721 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3781 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaataccc
     3841 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3901 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3961 ataacgagta gtttgtccag gctatattgg tactactgat aaaaccaact gtactggtaa
     4021 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     4081 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     4141 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     4201 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     4261 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     4321 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     4381 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     4441 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     4501 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     4561 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     4621 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4681 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4741 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4801 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4861 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4921 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4981 taacaaaatg aaaaaaaaga taagcacaag tgattgattg gttagatatt gatcacttgg
     5041 tgtttgtatt ttgaattttg cgatcaaaat ttgatctttg ttgtttccgg tcaatccttt
     5101 acaggttttc tgtattatcc gggggagtta agtctgtcga tgtccgacag acttttgatg
     5161 atggaaacaa catgcagcga taatgtgcat gctgttttgc aggtggaact cagaaggtga
     5221 actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc gatcaggcct gcaacatcaa
     5281 ggtgaaccac atcaaacggt gagatcccca ccccggccgt gaatgcactg ccgtcgttac
     5341 cggccagatt ctgacggtaa ccggcgcgca gctgtgccca tttccaggca ttaaattctg
     5401 tgccaatggc ggcaaactga cggttgctgt cggaggtgaa accgctggcc ggggtcagat
     5461 ccacatcaaa tgcagtggtg aacatcgcgt tgtgccagga gacaccggcc gtcacctgcg
     5521 ggcggatcct gaaggtttcc gtgataccgt tcacttcttt tgtctcaata ctacgggaga
     5581 tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc caggtccgta ctggcaccga
     5641 tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc gcctttgaag gcgctgctgt
     5701 cataatttct gaccagcacg ttgtagttga acaggtcgac gcgctggaat ttggggggga
     5761 agcccagaga ccatttctgc ccggccgttt ccagttccgt agccagagca atacccacat
     5821 cagtgatcag tgccgtgcgg gcacaggcct gtgatgtcag gcggcttttg tccacctcct
     5881 gcccggtgct gtttgccacc tcttccaggt aagtgaggtc agcatcagag actttaccgt
     5941 cgacactgac ggtgccgtgt gatttaacca tcagtgcggc agagaggcga tcaccgggaa
     6001 gggccgccac agcagaaacg cccagctgtg cggcggcatg tgtgttacgg aagtcccgga
     6061 ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg gttactttcg atggcgcggt
     6121 cgaaggcctt ccactcatca ctgatccggt cggcgttgtc ggtgatatta tccggattgg
     6181 agagctgcgc gccgacggac ggaagcagga tgctgggctc atgcgcgacg caaaatccac
     6241 gatgtatata tcagtactaa aagcgcgacg gcggaagaag ctctgaaact aatcggtgaa
     6301 ctgtacgcca tagagcacga tatacgcggg ttaccggtgt ctgaacgcct ggcggtcagg
     6361 caaatgcaga gtagaccgct cctgacttcc ctgtataagc tgatgcagga gaaagaacac
     6421 acgttatcga agaaatgtcg tctgggagat gcgttccggt atatcaggaa gcactgggct
     6481 gcgttgtgca acttctgtga tgatggcctg gctgaggtag ataataatgc agcagaaaga
     6541 gcgcttcgtg cagtctgtct cgggaagaaa actttttcga ggatcactca ttccgtgtct
     6601 gactgcttca gggtttaatt ttgcttctgc tgactgaatg atgattgggc agcaagtgca
     6661 atatcccaca taaatccagt caactcacga gccaccgccg taatggctac ttgtgatttt
     6721 ttcccttttg ctgtcagagt tcggtaacga gaacacaatc gttgttgagc tttccatgca
     6781 atatctttgg aatattgtgt tacagagtcc ggtcggtaat atataagcca tgctccaact
     6841 tttgcaggtg tacgataaga ccaggcggct tcgtaaagca gacgtctcag ttcgctgttt
     6901 ccaacttttg ttattcctct gggacgaata ctgtttccac tggaatattc acctggcacc
     6961 ag
//
LOCUS       DAJTNP010000158         6954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTNP010000158 DAJTNP010000000
VERSION     DAJTNP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..417
                     /gene="ydfJ"
                     /locus_tag="OO160_003798"
     CDS             <1..417
                     /gene="ydfJ"
                     /locus_tag="OO160_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5525744.1"
                     /translation="IVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITM
                     AEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLI
                     SALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            506..1966
                     /locus_tag="OO160_003799"
     CDS             506..1966
                     /locus_tag="OO160_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5525745.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2002..2205)
                     /gene="ydfZ"
                     /locus_tag="OO160_003800"
     CDS             complement(2002..2205)
                     /gene="ydfZ"
                     /locus_tag="OO160_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5525746.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2383..3069)
                     /gene="ydfH"
                     /locus_tag="OO160_003801"
     CDS             complement(2383..3069)
                     /gene="ydfH"
                     /locus_tag="OO160_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5525747.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3158..3904)
                     /gene="ydfG"
                     /locus_tag="OO160_003802"
     CDS             complement(3158..3904)
                     /gene="ydfG"
                     /locus_tag="OO160_003802"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5525748.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4041..6086
                     /gene="dcp"
                     /locus_tag="OO160_003803"
     CDS             4041..6086
                     /gene="dcp"
                     /locus_tag="OO160_003803"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5525749.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6131..6649)
                     /locus_tag="OO160_003804"
     CDS             complement(6131..6649)
                     /locus_tag="OO160_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5525750.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1751 a         1636 c         1750 g         1817 t
ORIGIN      
        1 attgtgctgg cgtggccaat gctttctatc atcgtagata aaagctatgc cccgagcacc
       61 attatggttg cactgattgt gattcataac tgtgcggtgc tgggattatt tgctctggaa
      121 aacattacca tggcagaaat gttcggctgt aaaaaccgct tcacccggat ggctatttca
      181 aaagaaattg gtggtcttat cgcttccggt tttggtccta tcctggcggg tattttctgc
      241 accatgacgg aatcctggta tccgatcgcc attatgatca tggcatattc agtgattggt
      301 ttaatctctg cgctgaaaat gccagaggtg aaagaccgtg atttaagtgc gctggaagac
      361 gctgcggaag atcaaccgcg tgttgtaaga gctgcgcaac cttccagaag tctgtaaacc
      421 ctcaatccct tctcttaccg gagaggggat ttttattcat ataacaaaac atatagcttg
      481 ccatatttaa atttaaggaa ttgttatggg aaataatttg ttatcagcaa aagcgacact
      541 ccctgtttat gatcgtaata acctggcccc aagaattgtt catttaggct ttggtgcatt
      601 tcaccgtgcg catcagggcg tgtatgccga tattcttgct acagaacatt tcagtgactg
      661 gggatattat gaggtcaact taatcggcgg cgaacagcaa attgccgatt tacaacagca
      721 agataatctt tataccgttg cggaaatgtc ggccgatgcg tggacggctc gcgtcgttgg
      781 cgtcgttaaa aaagccttgc acgtacagat ggatggctta gaaaccgtgt tggctgcgat
      841 gtgtgaaccg caaatcgcga ttgtctctct gacaatcacc gaaaaagggt atttccactc
      901 tccggcgacc ggacagttaa tgctcgagca cccgatggtc gctgccgacg tgcaaaatcc
      961 ccaccagccg aaaacagcaa caggggtgat tgttgaggcg ctggctcgcc gtaaagcggc
     1021 aggacttccc gcatttaccg tcatgtcatg tgacaacatg ccagaaaacg gtcatgttat
     1081 gcgtgacgtt gtcacttcct acgcgcaagc cgttgatgta aaactggcac aatggatcga
     1141 agataacgtg actttcccat caacaatggt ggaccgtatt gtgcccgcag tgacagagga
     1201 tacgctggcg aaaatcgaac aacttaccgg tgtgcgcgat cctgctggcg ttgcctgtga
     1261 acctttccgc cagtgggtta tcgaagataa ctttgtagcc ggacgtccgg aatgggaaaa
     1321 agcgggagcc gaactggtta gtgatgtgct gccttatgaa gagatgaagt tgcgcatgct
     1381 caacggcagt cattcattcc tggcatatct gggttatctt gctggatatc agcacattaa
     1441 tgactgtatg gaagatgaac attatcgtca tgcggcgtat ggcatgatgt tgcaggaaca
     1501 agcgccgacg ctgaaagtgc agggcgttga tttgcaagat tacgctaacc gattaattgc
     1561 acgctatagc aacccggcgt tacgtcatcg aacctggcag attgcgatgg atggcagcca
     1621 gaaattgcca cagcggatgt tggattctgt tcgctggcat ctggcgcatg acagcaagtt
     1681 cgatctgctg gcgctgggcg tcgcgggttg gatgcgttat gtcggtggtg ttgatgaaca
     1741 gggaaatccg atagaaatca gtgacccact gttacctgtt attcagaagg ctgtacaaag
     1801 tagtgccgaa gggaaagcgc gcgtccagtc attgctggcg attaaggcaa tctttggtga
     1861 tgatttgcca gacaatagct tgtttactgc aaaagtgacg gaagcgtact tgtctttatt
     1921 agcgcatggc gcgaaagcga ccgtggcgaa atattccgtg aagtaaaaaa agcaatgccg
     1981 ccatcggcgg cattcacaca cttagttcat gccgagacga atcaggtcca gaggtgccag
     2041 tttctcttca caaccttcaa caactacggt ttttccgcgg cggatttgct cagcggtcag
     2101 accttcagta tcaatcgaca ggatcttgcc agtgtgaccg gtgccgctaa ccataacacg
     2161 gctgccagtg gtgattgcgt tacggttacg atcgtaggtc gtcataggtg ttttctcctt
     2221 tctgatttac agtcgaagcc cacgtaacgg gcgcaatata aatactcctg tcgagtgaaa
     2281 tattttttgt ttttgatcaa gctcacatct tttttgatgc atgcatgagc cgtaaggcgg
     2341 ggggaaaagc ctctcatccc ggatgggatg agagtaaatg aattactctt cgctaaacca
     2401 gtcgctgttt tcctggcgga tctggcgcac ggattcgctg atttcctgca aatgttgcgt
     2461 cattgcacgt tctaccgcat cgccatcacg tttttgcagg gcagagaaaa tatcaagatg
     2521 ctggcgtaac agcatttctg gtggagaaac atggtcgaaa ctcatatagc gcacgcgatc
     2581 aacggtcgct ttcaggttct caatggtatc ccacgccagt tgacagtcgg caatctgcgt
     2641 caggagttga tggaagttgt catcaagttc aaaaaaatca tccagttgct tgcgctcaat
     2701 ggcaatgcgt tgctggtgaa gattttgttc cagttggtag cactggcttt cggtaatcat
     2761 gctcgccgcc cgacgcgcca ccgcgcactc aatggcctga cggataaaac tgccgttgcg
     2821 cacctgggcc atggaaattt tgttgacgta gctgccacgc tgcggacgaa tttgaatcag
     2881 gccgttttcc gccagtttaa taaaggcttc acgaaccggc tggcgtgaca cattgaaacg
     2941 aacagaaact tctttttccg acaacggtgt gcctggtgca atcaggcaat ggacaatgtc
     3001 gcgacgaaga atacgataaa tctgctgatt gacaggctgt gtgggattaa gttgcgtttc
     3061 gacggtcatt cgttcttgct tgtgagtgag ttaactgcat gagtctacca cttttttgtg
     3121 acaagcaata acccggcagt tacgccgggt ataaaaatta ctgacggtgg acattcagtc
     3181 cggcatagct ttgggtaacc ggcatcattt ccagggtatt gatattgacg tgagcaggca
     3241 gcgttgacac ccaccagacg gcttcgctga catcttctgg cgtcaatgca acggtatttt
     3301 gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg gacattggaa aactcggtgc
     3361 cacccaccag acccggttcg atgtcggtga cgcgcaccgc cgtaccatgc agatccgtac
     3421 gcagattcag gctaaactga cgaacaaacg ctttcgtcgc accgtaaacg ttaccaccgg
     3481 catacggcca gctgcctgcc gttgagccaa tgttaataat atgaccatga ttacgttcaa
     3541 ccatacccgg taagacggcg cgcgtcatat atactaggcc tttgttgttg gtatcaatca
     3601 tcgtttccca gtcttcaacg ctggctttat gcgcaggctc catgcccaac gccaggccgg
     3661 cattatttac caggatatca atattgctcc actcggcagg gagcgatgcc agcatctctt
     3721 caatagcggc gcggttgcga acgtccagtt gggcgatata cagattatct cccagttcgt
     3781 ctgttaactc ctgcaaccgc tcctggcgac ggccagtggc gataacttta tgcccttgtt
     3841 gaataaaacg acgagtaatg cattcaccaa aacctgccgt tgctccagtt actaaaacga
     3901 tcatctcact gttcctcaac gcttttgtgt gtcaataaca tagcacgtga tgcttaagag
     3961 cgggtaatac tggtttgcaa gattgcactc cgttatcagt aagcctacgc tatggaacac
     4021 ctcgcgttca ggagaagaaa atgacaacaa tgaatccttt ccttgtgcaa agcacactgc
     4081 cgtatctggc tcctcatttt gatcaaattg ccaatcatca ctatcgcccg gcattcgatg
     4141 agggaatgca acaaaagcgg gcagaaattg ctgccatcgc gcttaacccg caaacgcctg
     4201 atttcaacaa tactattctg gcactggaac aaagcggaga attaattacc cgcgttacca
     4261 gcgtcttttt tgcgatgact gcggcgcata ccaatgatga attacagcgt cttgatgaac
     4321 agttttccgc tgaactggcg gaactggcta atgatatcta tctgaacggt gaattattcg
     4381 cgcgggtaga tgctgtctgg cagcgccgtg aatccctggg gcttgatagt gaatccatcc
     4441 gcctggtgga ggttattcat caacgttttg tccttgccgg agccaaactt gcacaagctg
     4501 ataaagcaaa attaaaagta ctgaatacag aagctgcgac cctgaccagc cagtttaacc
     4561 agcggttact tgcagcaaat aaatccggcg gtctggttgt gaacgatatc gcgcagctgg
     4621 caggaatgag tgagcaagag attgcgctgg cggcagaggc ggctcgcgag aaaggtctgg
     4681 ataacaaatg gctgattccg ctgctgaata ccacccaaca accggcgctt gccgaaatgc
     4741 gcgatcgtgc gacgcgtgaa aaactgttta ttgcgggctg gacgcgagcg gaaaaaaatg
     4801 atgccaatga tacccgcgct atcattcaac gtctggtaga gattcgcgca cagcaggcga
     4861 aactgcttgg ttttcctcat tatgccgcat ggaaaatcgc cgatcagatg gcaaaaacgc
     4921 cagaagcagc actcaacttt atgcgggaaa ttgttccagc ggcgcgtcaa cgtgcgagcg
     4981 atgaattagc ctccatacag gcggttatcg ataagcagca gggcgggttt agcgcgcagc
     5041 cgtgggactg ggcattttat gcggaacagg tacggcggga gaaatttgat cttgatgagg
     5101 cgcagctcaa gccatatttt gaattaaaca cggtgttaaa tgaaggtgta ttctggaccg
     5161 cgaatcagct cttcggtatt aagtttgtcg aacgttttga tattccggtc taccatcctg
     5221 acgttcgtgt gtgggaaatt tttgatcata atggcgtggg actggcgtta ttttacggtg
     5281 atttcttcgc ccgtgattca aaaagcggcg gtgcatggat gggcaatttt gttgagcaat
     5341 caacgcttaa tgaaacgcat ccggtaattt ataacgtctg caattatcag aaacccgctg
     5401 ccggtgagcc tgcgttgtta ctctgggatg atgtaataac cttattccat gaatttggtc
     5461 atacgctgca cggccttttt gcccgccagc gttatgccac gctttccggc accaacacgc
     5521 cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca ctgggcaacg catccgcagg
     5581 tattcgctcg ctacgcccgg cattatcaga gcggggcagc aatgcctgac gaactgcaac
     5641 agaaaatgcg taatgccagc ctgttcaaca aagggtatga gatgagcgaa ctgcttagcg
     5701 ccgcacttct cgatatgcgc tggcattgcc tggaagaaaa cgaagcaatg caggatgtcg
     5761 atgattttga attgcgggcg ctggtggcgg aaaatatgga tcttcctgct ataccgccac
     5821 gctatcgcag cagttatttc gcccatattt ttggtggcgg atatgctgca ggttattacg
     5881 cttatctgtg gacgcaaatg ttggccgatg atggttacca gtggtttgtt gagcagggcg
     5941 gattaacgcg tgaaaatggg cagcgttttc gcgaggcgat cctttccaga ggtaacagcg
     6001 aagatctgga acgcctgcat cgacaatggc gtggtaaggc tcctcagatt atgccgatgc
     6061 tgcaacatcg tggcttgaac atataaacac caccaatggc tggcctggag atatcgccag
     6121 gccagcctgg ttatagcagt aattgcagca gctgggcgag ggcaaacctc accgctaaag
     6181 ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc agtgtagttc tggcctttaa
     6241 tatgccacgc gaaccagacg gtacccgctg gcgtaccatc ttcaccgccc tccggtccgc
     6301 cgtagccggt aatggcaata ctgacatcag catccgcacg ctctatggca cctgttgcca
     6361 tttctgctgc cactttctca ctcaccgcag aatatcgttc aagagactgc tggcttacgc
     6421 tgaggatttt catctttgcc tgatcggtga aagtaacaaa gcctgcaccg taaaatttgg
     6481 gtgtatcttc agctgcacac agggcgctag ccaacttacc gccggtgcat gattcggcgg
     6541 tagttaagcg taattgatcc gcgataagtc tttgactcaa cgcggatgtc aggttttcaa
     6601 tagtgtcggt atctgcgagt tgtacaattt tatctttatt aatgttcact gttttttgtc
     6661 cacgctatta tatgtaaagg tgatttgctt tttctgtatg ctcgtccaat ataataattt
     6721 tcaaacatat aaatccgatt attctgaaaa catgtttaag actcttcaat cttcttaact
     6781 ttagtgagtc aaattctctg aatatttgac agctagtggg ctgattttaa atgccttata
     6841 acaattggca gtattcttat ttggcaatta taaaaatagc aaccagactt aataagtcaa
     6901 accaaacgca attaaggagt tagtatgaaa tttcagtcca ttgtattagc aagt
//
LOCUS       DAJTNP010000159         6801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTNP010000159 DAJTNP010000000
VERSION     DAJTNP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO160_003805"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO160_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5525751.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO160_003806"
     CDS             2089..2238
                     /locus_tag="OO160_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525752.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3016..3456)
                     /locus_tag="OO160_003807"
     CDS             complement(3016..3456)
                     /locus_tag="OO160_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5525753.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3674..4063
                     /locus_tag="OO160_003808"
     CDS             3674..4063
                     /locus_tag="OO160_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525754.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4107..4511
                     /locus_tag="OO160_003809"
                     /pseudo
     CDS             4107..4511
                     /locus_tag="OO160_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4618..5454)
                     /locus_tag="OO160_003810"
     CDS             complement(4618..5454)
                     /locus_tag="OO160_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5525755.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5454..5729)
                     /locus_tag="OO160_003811"
                     /pseudo
     CDS             complement(5454..5729)
                     /locus_tag="OO160_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5804..6277)
                     /locus_tag="OO160_003812"
     CDS             complement(5804..6277)
                     /locus_tag="OO160_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5525756.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6298..6801)
                     /locus_tag="OO160_003813"
                     /pseudo
     CDS             complement(6298..6801)
                     /locus_tag="OO160_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1589 a         1761 c         1771 g         1680 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tatacatcac ttttacatca cttatacatc acttttacat
     2641 cattccttca gctcatacgt cactttcatc ctctgtgcca tttgtacatc acccccttcc
     2701 tgaacgtgct gacagcgggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     2761 attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga cttaccccgg
     2821 ctgaatggtg tgaatttccg cgctatgcac ttgtgcgcat actcatgcat gccgtaaaaa
     2881 cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     2941 cgtcagaaac gccctgaggg cctctcaggc ggtgctgata acagatgtta tggtaaatca
     3001 gagtcacctg cactgttact ctgaaccgga gcgaaacagc cgcgaccaga atccccgctt
     3061 tttttcctgt gccagcgcgt cagccagacg gctaatttcg gcctgtagtt gttccctctc
     3121 ctcatttcgc aggcggtttt ctgcttcccg cgccatcttg tcttcaagca ttagggtaac
     3181 agtctgctgc agagcgtcca gtttctgctg aatgacgtct agctgagcat tttccgcctg
     3241 gctaacgaca gatttggagt gtccctcctt gtgccttttt ggtgtctcag aagtgcgcag
     3301 ctcgccatag gcccgaatca gttcactggt ctcgaactcc cggcgatcat tttttcctgt
     3361 gcgatagctg acaaggcctt tagccatgtc acgatataat tgcgagcgcg actttcctgt
     3421 taatttcagt gtctcttgaa ctgtgtgcca tgccataaaa gtgacagtgt ctcctgtgtg
     3481 taccgtattg taacagcaga acatattact gttttaacac aaaaggtctg aggctgcccg
     3541 tgaggcgtga aaaatacaca aaaaacctgt tgacggtgtg gttttgacgg gcgtagtatt
     3601 tcgaatactg tatataaaca cattgttata aatacagttt ttgctgtttt taaaagtcaa
     3661 agaggataat tttatgagta cagagttaaa tagcggtact gtcggtggtt ttggtcacat
     3721 gggaccaaat gacccctaca atgatggtca tagcggctct ggaagttcgg gaggtagttt
     3781 taacggtggt ggtaatggtg gccatgataa tagtagtggt tcatcttata ctgcatcctt
     3841 tggtctcatc actggccgac acccgataac tgtcccaggt acgaagaata ttaattctgt
     3901 cgatctttct gctgtcatta ataacattat acatcatgaa ggtgcacgca ataacatgta
     3961 cctagatact gcgaacaatg taagagtggg agtgacgggc actggctggc aatgtctagc
     4021 aacggcaggc atttcggctg agggtaaaag aactttccgc taagcgatag actgtatgta
     4081 aacacagtat tgcaaggacg cggaacatgc ctcatgtggc ggccaggacg gccagccggg
     4141 atcgggatac tggtcgttac cagagccacc gacccgagca aacccttctc tatcagatcg
     4201 ttgacgagta ttacccggca ttcgctgcgc ttatggcaga gcagggaaag gaattgccgg
     4261 gctatgtgca acgggaattt gaagaatttc tccaatgcgg gcggctggag catggctttc
     4321 tacgggttcg ctgcgagtct tgccacgccg agcacctggt cgctttcagc tgtaagcgtc
     4381 gcggtttctg cccgagctgt ggggcgcggc ggatggccga aagtgccgcc ttgctggttg
     4441 atgaagtact gcctgaacaa cccatgcgtc agtgggtgtt gagcttcccg tttcagctgc
     4501 gtttcctgtt tggggtcgtt tgcgggaagg ggcggaatcc tacgctaagg ctttggccag
     4561 cgatattctc cggtgagatt gatgtgttcc caggggatag gagaagtcgc ttgatatcta
     4621 gtatgacgtc tgtcgcacct gcttgatcgc ggccgcgata gctagatcgc gttgctcctc
     4681 ttctccatcc gcgttccaag ctgcggaaag gcacccataa gcgtacgcct ggtcgagcag
     4741 gcgacgcgga tcgacgtcca gcgcacgaga gaatgcgtcc gccatctgtg caatgcgtct
     4801 aggatcgaga caaaggtcgt ctctgtcagc cggatcgtag aacatattgg cggcgccaaa
     4861 gcccacttca ccgaccagac cgacgggatc tatcaccagc cagccgcgac tggagaacat
     4921 gatgttttca tgatgcagat cgccatgtag cccacgcagt tccgaggcat tgctcatcat
     4981 ttgatcggct ataatcgccg cgtggacgta gtcagtttga caacctgcgt tttgatcatc
     5041 gcgcgcccgc tgaaacaaag ctgcaaagcg atcccggatc gggagaaggg cagaaggcag
     5101 gggttcctca gatgcggcat acagcttcgc cattagttcc gctgcaattt cggtcgcctg
     5161 gtagtcgccg tgctcggcaa cgatgtgaga gagcattcgc tccccggcat attcgagcaa
     5221 catcagattg ttctcacgac cgagcaaccg gactgctccc ctcccattgc gccataccag
     5281 atagtcggcc ccgcgcagtt catcagcaat gtcttctata ggtttcaatc ccttgacgat
     5341 tgcaggagtc ccgtctggca atgaaacttt ccaaacgagg ctggaaaagg tgtccgcaat
     5401 gagaacaggt tgcgaaacgt gccaatgagc aggaaaaaca ggcggcatga acatcaaccc
     5461 caagtcagag ggtccaatcg cagatagaag gcaaggcgtt cgcggtcggg ggcttcgatc
     5521 cccaatacat tgaataggac agcgaaggcg cgctctgctt catctggcgc tgcccagttc
     5581 tcttcggcgt tagcaatcat gagtgccaaa tcggcatagc gatctgctgt tccgagccgc
     5641 ccaaggtcga tcagacccgt gcattgaaga gttttagggt ccaccatgaa gttcggcatg
     5701 cagggatcac catggcaaac aaccatatcg tcgctatcaa tcgcggaaaa aggcgtagtg
     5761 cgcgaagcga acgaagcttt ttgccgtcga ttgcatagct ttgttaaccc tttttccaaa
     5821 tttgatagca atagttaatg tttgaagtaa agtgttgctc aaaaacaact tcgaaggtat
     5881 ttggaatact cgggaagaaa acatccccct ctggctcgat gtcgatcgtc gataagtgga
     5941 gcgtagaggc catgggtaat gtttctcggt aaatttctcc gccaccagac actataacgt
     6001 gaccggtgaa ttcagctagc ctgtccatgg cctcttcgat tgactgaaat acaactacat
     6061 tgtcatcatt tgatgtccaa cctgagcggg taacgaccgc gtatttccta ttggggagtg
     6121 cgcccataga ttcaaacgtc ttgcgaccca ccagaagcca ctgattgtag gtcaatgctt
     6181 taaaaagtag ctgctcccct ttcgcggacc agggtatgtc tggaccgcaa ccaatcacgc
     6241 cgtttttcgc tttcgcagct atcaatgata ctttcaaggt tcttatcctg ggttaacggt
     6301 gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg
     6361 cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc gggacaccac
     6421 atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aacggacatt gatcaaccga
     6481 taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga gcaaatccgc
     6541 tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc atgcaccctc
     6601 ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt tgagccaaat
     6661 gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa
     6721 ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa
     6781 ccagtcagaa tgcgattcac c
//
LOCUS       DAJTNP010000160         6647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTNP010000160 DAJTNP010000000
VERSION     DAJTNP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(296..730)
                     /locus_tag="OO160_003814"
     CDS             complement(296..730)
                     /locus_tag="OO160_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5525757.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(718..1119)
                     /locus_tag="OO160_003815"
     CDS             complement(718..1119)
                     /locus_tag="OO160_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525758.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1379..1948)
                     /locus_tag="OO160_003816"
     CDS             complement(1379..1948)
                     /locus_tag="OO160_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5525759.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2693..2836)
                     /locus_tag="OO160_003817"
                     /pseudo
     CDS             complement(2693..2836)
                     /locus_tag="OO160_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3170..3436)
                     /locus_tag="OO160_003818"
     CDS             complement(3170..3436)
                     /locus_tag="OO160_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525760.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3577..3783
                     /locus_tag="OO160_003819"
     CDS             3577..3783
                     /locus_tag="OO160_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5525761.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3878..4480
                     /locus_tag="OO160_003820"
     CDS             3878..4480
                     /locus_tag="OO160_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525762.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNINYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5680..6597
                     /locus_tag="OO160_003821"
     CDS             5680..6597
                     /locus_tag="OO160_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525763.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT         1647 a         1735 c         1570 g         1695 t
ORIGIN      
        1 attctgacca gacggttcag gctgtacgct taccactatt ttatccctgt tgaatttggt
       61 taagccggtg cagacgcgcc gggaaacgca cacctgaacc ggcacaataa aatacaatac
      121 aaccccctgt tactggagat acgaatggaa tgacgaggca gcctgtcagt cacagggggc
      181 tgttggcata aaatcaacgg aaaggccacc aacaccggaa ttaagctcca cagtccccgc
      241 actggcaatc cattcccggg aaaggcatca ccgcaaactg ctccggaggc aaccattaca
      301 gcctctgtaa cggattatca gccctggcat cttccgacgg cacctcctgc cgcccccacg
      361 ccctgctgta taccccggta ccgcagcgtg ggcagcactt ttccccctca taatcttcgt
      421 cacacatcac acaaaaccac tttctcacct tcaccgtata atccggaacc gtcatgccct
      481 gcagatgcaa acgacgggcc tctttctctg ccgggtcaag ccaggtacat cgcagcagga
      541 ttttttcagc gacagtcatc tggtcatgtt caaaccacgg tggttgtccg tcattgcccc
      601 gctgaagtgt taacgggcat ccacaggaca cacagcacaa aacgtcctgt cggttgtgct
      661 tcgcagcttc tttcgccgta acaaaatggc ctttgctgtc attcgccata taacagttca
      721 gaattcgcat atttcctcct gagttgtccg gggaatactg aatcctccgg tctggcagtg
      781 tgtgcagtac tgctccccat aataatcgtg gtgacattgt ctgcagtgcc agctggcttt
      841 acgcaccacg ggtaaggcat ccggtacgaa ttgctgcaga cgtttaatca actgtatttc
      901 tctgcgctcc ggcctgacat aagggcactg ttgaccgtgc tctgtcagcc tgtcgtcagt
      961 gtgttcaaac cagggaagtt cagtttcgta ttgcggatgg tatctgagtg cactgccaca
     1021 caagtggcag gtgtagttag cataaggtgc ggcctgtgca gtacgggcgc ccgtcagacg
     1081 tccgttgcca tcaagagcga taaaggattt tgcgtacata gtcatatacc tcagccgcca
     1141 gacgacacgc aggcaacagc cgtcccggta cgggcagcgt ggtcagggtg tgaatggcgg
     1201 ttcagttaaa atctggactg gagtttgggt aaagcaggct ggttgctgtt acagcactgt
     1261 ggttgtcaca caatggaaca ctgacgtaaa aaacagtcag tgggcgagtg gatacatcgt
     1321 tgccgccaga caacaaccca tacttttggt ttgccggata ttggcgcgcc agtcgtgctc
     1381 aactccggct acagccaaaa ttgcgctcac cggtgcagtg agctttggta tgctccttcg
     1441 ccagatagtc agcacgttcc agcacttgct gaaagccggt gtcatcatta cgcgccagcc
     1501 acaccgccgg ccaggccgga aaatgcacca gcgtggcatg gcacccgaca tccactccca
     1561 gggcactgca ccacgcctgt ttaatcatcc cggccagaga ctccggagcg cggtaatcac
     1621 cggcacgaca ccaggtatcc cggttgacca gcagcatcag gtgatagtgt tttttacctt
     1681 tgcactcccc aaactcccgg gcccagacgt aatgcagggt tgtgggatgc acgcgtttac
     1741 cttcccgatg cttacgtttc tggtaagcgt cgattcgggc tttcagggca ttgatgaagc
     1801 gggatatcac agctgcgtcc gttgctgccg gtacatccgg gagacgcagg tcaacccgca
     1861 atgccgtcag gcggggatga acattcagtg cgtgccgcac cgtctcacga atacgttgct
     1921 gccagaaggg gttgtatttg taggtcatgg ttaaatctcc gtatggttca tacggaatag
     1981 ccacgtcgta aaaaatacgc agagtccctg aagtgacctc cggcaggaca ctgtctcagg
     2041 cactctgtga taacccgaac ggtcgtctcc ggactgacgg ttgaattttc agcgtcgttt
     2101 tttaatgtaa aaaaccggtt acgggtaagg ctgtgaggag ggagtgatgg tgatacgcaa
     2161 aaagaagtgc agggactgtg gaaacgcgat tactcataat acagtgtgtt gcccgtattg
     2221 tggttctgtc gaccccttcg gctactaccg gaatacagac cgtatcgtta ccatactgct
     2281 ggcgctgatt attgtggtcc tgctgactac tgtcagcgtc agtgtgtgta tactgtgtag
     2341 ctggtgagat aacggttcat gtatcccctg agctgacagt gtaccgtgta tattaagcgt
     2401 aaccgacagg ttctccttca taccatatta aattttccag ggattgccgc tgccttattt
     2461 cgtcccatac aggaaaactc atctgggttt ctgacaaata cgcgaaactc acagcccctc
     2521 aatatcaacc acacgttgta agccggtaat acctgaaaat cagcctatta ccgcccacgc
     2581 ctccggcacc attccctttt gatttcgaat tctttgttgt cgctataccg cttaatgttc
     2641 ccggtcctcc ggtgcagata acaccggagt catcgtagcg ctgcttcaat attcaggtga
     2701 cgtatttcca gacggaagcc ggcgcgcgat cgtaaagttt gttcattgtc agttctgcca
     2761 gccggttgtc aacggcagag ttaaaggctt ccagctccgc aagggtaagt ttataacgcg
     2821 tgtgggaatt cacttttccc gcgtctcctg acgtattacg ggctgccact tacctggtgt
     2881 cccgcaatac agccttagtg ttccgttatc ggggatccgg acgcaccgca acaaccgggg
     2941 aagtatttct ggtttagagc agtgtcaaga gggatttctc agcccctcac tcaccggctc
     3001 agtctcaggg caggagcatt aatacgctca gttcatgtct tatacaggaa tgggcacagg
     3061 gggagctggc gtgtcactgc tgattttacg catcagcgtt catgtatctg cactctgata
     3121 catgaacgat tcacagcgct ctgttaccgg agagatttac tctcttagct tactctggca
     3181 aatcctttac gttactctct gatgacttct tatatttata ataccaatca gacagcctta
     3241 tcccatcggt gatatcagtc tcctcctcgc caggaacggg ctgactcact gtaactacct
     3301 ccgaaaacaa ctctgcaatt atcagaacgc ctgcttctct ccctgtcctc acgaaaacta
     3361 tcccctcttt atcgcgcgtg cgtgcggaag catcttttcg caacaaccac ccggtatacc
     3421 ggtacggctc agccaccgca acctccccgt ttatctcctg gcggtcacat tcccgattat
     3481 tttttacgtt tctccccggt tgttatgccg gtgaaggtgg cgcgctgttt tcatgaccac
     3541 accggctggt ttaacaacat ccggaggaac attctcatgg ctaccccagt ttcactgatg
     3601 gatgaccaga tggtcgacat ggcatttatc actcaactga ccggcctgac agataagtgg
     3661 ttttacaggc tcatcaggga tggggccttt ccggctccca tcaagctggg ccgcagctcc
     3721 cgctggctga aaagtgaagt ggaagcctgg ctacaggcgc gtattgcaca gccccgcccg
     3781 taatttctga ccgttatccg ttcacccgca gcagacgtaa tccccggcct gccggcggca
     3841 ttctgctgcc tgttatatcc ccgtgaggaa tatgaaaatg aaacaacagt atcagacccg
     3901 ctacgaatgg ctccacgaaa gctaccagaa atggctgacc ggcttcaccc ggcacgccgt
     3961 atcctggggc gtgtgtcatc cgaatatcaa ctacttccat aatctgacgc ccggttgggt
     4021 gtcattcaat ggtgaaaaac cggagattgc catcgtaccc cagagcctgc accggctgat
     4081 ttatggccct gacaaacggg ccacgccgcc cctggatgat gatctgatag tgaatttatg
     4141 caccagtgaa catctgctgg ttcatcatcc gatgctggaa ggcattctcc tgtctgaatg
     4201 tgaacgtctg aggcagcgtt ctctggcaaa taaactgatc agcctcttcc gtcagtttgg
     4261 tggcacggag ttgcgcctca aactggtctg gctttgctgg cttgatttaa tgaccggaaa
     4321 cagccttgaa gactggaaag agaacctgaa acggaaatca gaaaaagagc tggaggaatg
     4381 gatcatcaac cgtcagaggc agagcgcagc actgacggat ctgatggatc agtacgtgct
     4441 cctggcatac cgcacaacgg ttgacgatag ccgcaactga tgccggttgc ttccggaagg
     4501 tgtgacaaaa tacggaaatg atgatgctcc cggcccccat gctcacctgc agagcgtgtt
     4561 cgtcgtcatc atactgagcc atgacgggcg tcttaagggc atcctgtcat tgctgagcag
     4621 gcaacaccac cagaggagcc acggacaggg atgttcttta cagcccgata cacgccaggt
     4681 tctcagcaga ctgaatgtgc aggtgcggtt attcaccaga aatggcatgc tgcggtacat
     4741 acccatcctg gtgacaatat gtacgtggcg acagcgatcc cggcactgtg gaagagactc
     4801 actcagcctg caggatattc tttccggcat ccgggaatgt ccgcctgaat gcaaacagga
     4861 tgatatttga atccgtaata ctgttctttc atcataaata atttatgcag atacagttaa
     4921 ccgttacagt agccggtttg ttttaccggg aaataatatc cgtcagactg ataccggaac
     4981 atatctgaaa cacgttatga tgttttgtta tcagaaatgt tgtatattct tatccgtggc
     5041 gacagtttaa tgccctctgg tatcacctga taatgtatat cctgcgatga ttcgttatat
     5101 ttacgggaca cgcctctttt cattgtgctc cttttcagac ttcattttcc gtaagtgttc
     5161 cccggcataa aggaggtacc tttctggcgt ttataactct ctttatcggt gacgccggta
     5221 atacagggaa taccacaccg acaaacgagg tattaacaga cgatatttca gtgtccataa
     5281 tttttctgtc cggttaaata acgtcagtct gctgccctct gactgtaatc atcaaagccg
     5341 gaatgcatac cgggttacaa cgtaaaaaaa gtgatgctgg cccccggtgc agctggcggg
     5401 cgaaacatgt ttacctttca ataccccctg tctgcagtct ccactcttcc atcctcttaa
     5461 gctacgtcgg ccacatgagt gcacttccat gataacaggt attcctgtca gcataacgaa
     5521 gccggttacc ttcccttcct cctgcatttc ctgtcagaat gactatacag cgcgcagatt
     5581 gcctttttat tgaccatctt aatataccgt tataaacttc tttttatcat attattatca
     5641 ccttattatg tttaattttt aagcagtcgg agaaagagga tggttcagaa aattttgtcg
     5701 gataaggtta tgaatgaaag aacaaatgcg tattactctt attatcttgg agagcgtaat
     5761 atctccgtac tgccccttaa tgtttatgat cccccggaac gttttatcgc ttacataaaa
     5821 aaaaacagag aaaatctgaa catcacactt tcagattttg aacttgaaca aataatttct
     5881 ggtatgcgtc tgaaggccct ggccttcctg gtccccctgg aaaaaatatc ctggatcgcg
     5941 ggcagtgaac gcgcatgctt attttcctgg tatctgttga tgcagtttat ccagaataac
     6001 agggcaaaaa taagcgcaga cttgcttcaa aagaataaac tgtatcttaa agaagagtat
     6061 ctggaaggga atgccttccc gtcggactca tcgacacagt tcagacaaat actcagagtt
     6121 ctcgatattc tttctgataa gaatttgcgg gatgagtgga taatccagac caaagacaga
     6181 tggatgcggg catttaaatc aaagagtcct ttcagttacc tcctcccgga gaacgaacat
     6241 gaatgcatat ggacatggaa ttatttgaag ggaaaaaata ttgcactgga aaaactggcc
     6301 agttttcctg gttctgctga tatttaccac gcaattcacc tttcatttga catatgggtt
     6361 acctgtccac tcacctcacc tgacgatata aaaaatttca gaaacagttt caataaggcc
     6421 aaggcgcagc gcaagtacaa aaaaatgcag gaagataaag tcaatgtaca gttttttctt
     6481 gacgcagaaa ccagggcgca gctcaaagaa ttatcgaggg tcagaagact cagtaccggc
     6541 gaaatgctgc atgacctgat tgttgaagag tataaaaggt accgacattc cagataattt
     6601 cagacatata ttatctccgt gagtcccccg ccacctttcc ggtgtgc
//
LOCUS       DAJTNP010000161         6558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTNP010000161 DAJTNP010000000
VERSION     DAJTNP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            431..1819
                     /gene="narU"
                     /locus_tag="OO160_003822"
     CDS             431..1819
                     /gene="narU"
                     /locus_tag="OO160_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5525764.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIAMIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1901..5641
                     /gene="narZ"
                     /locus_tag="OO160_003823"
                     /pseudo
     CDS             1901..5641
                     /gene="narZ"
                     /locus_tag="OO160_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5638..>6558
                     /gene="narH"
                     /locus_tag="OO160_003824"
     CDS             5638..>6558
                     /gene="narH"
                     /locus_tag="OO160_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5525765.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDLYRVGSLTAAACHGLQ"
BASE COUNT         1563 a         1723 c         1704 g         1568 t
ORIGIN      
        1 aaataaacaa tacttactat aataacgatt attacaatca ggcaataaga gaggaaccga
       61 tagactttct ggacagatcg ttttcctcca attcatggcc tttttagtga ataaatcatc
      121 actaattcta taagtgtctg gtcttgttga gaatatagtt aatatgtaac cctttagatt
      181 cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt ttgaaggtta
      241 gcttgttagt tacatttagt aacacatatc tggctactta atccccattc tgcccccgcg
      301 gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc ttaattctaa
      361 taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat gtgtgcatat
      421 gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac gcgactggaa
      481 accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa gaaacctctg
      541 gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta gcgcagttac
      601 cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt tattaaccgc
      661 attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg tgcctatatt
      721 cggtggacgc cgatggccgg tttttagtac tgcaatcctg attattcctt gcgtctggct
      781 cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta tcgctttgct
      841 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
      901 aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact taggtgtaag
      961 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
     1021 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
     1081 ggtaccgcta ctggcgattg ccatgatcgc cgcctggtca gggatgaatg atatcgccag
     1141 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
     1201 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
     1261 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
     1321 tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg
     1381 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
     1441 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
     1501 gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
     1561 aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga gagaagccgt
     1621 caccgaaacg gcggcaacgc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
     1681 tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
     1741 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
     1801 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accatgtcgc atccgacaag
     1861 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     1921 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     1981 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     2041 ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     2101 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     2161 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     2221 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa
     2281 acaacacagc gatccggtac tggcgtgggc atcgattatg aatgatccgc aaaagtgcct
     2341 gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc aggaactaaa
     2401 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     2461 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     2521 gtcgctgctt ggcggcacct gttaaagttt ctacgactgg tattgcgacc tgccgcccgc
     2581 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     2641 cagctacatc atcgcttggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     2701 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     2761 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     2821 gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     2881 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     2941 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     3001 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     3061 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     3121 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     3181 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     3241 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     3301 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     3361 ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     3421 tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg aatttgccga
     3481 taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg ttaaccactg
     3541 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     3601 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     3661 cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag
     3721 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     3781 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     3841 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     3901 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     3961 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg
     4021 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     4081 acagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     4141 cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct
     4201 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc
     4261 caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc catttattca
     4321 tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg aaatctacaa
     4381 aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt
     4441 ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     4501 cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga atattgtggc
     4561 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     4621 caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa tcgatttcct
     4681 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     4741 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     4801 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     4861 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     4921 ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     4981 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     5041 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     5101 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa aagcacttaa
     5161 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     5221 gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact
     5281 gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc tgactgcccg
     5341 cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg
     5401 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt
     5461 cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg
     5521 ctttaactac tacggcaccg tcggttcgaa ccgcgacgaa ttcatcatga tccgcaagat
     5581 gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg
     5641 aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg ctgccatacc
     5701 tgttcggtga cctgtaacaa cgtctggacc ggacgcgaag gcatggagta cgcatggttt
     5761 aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga tcaggaagag
     5821 tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct gggcagcaag
     5881 atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagcagat tgatgattac
     5941 tacgaacctt tcaccttcga ctacgaacat ttgcatagca caccggaagg caaacatatc
     6001 cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt gatctggggc
     6061 ccaaactggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc
     6121 gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat gtacctgccg
     6181 cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag cggcgctatc
     6241 tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg tggctggcgt
     6301 ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag cggcaagtca
     6361 gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa
     6421 acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga ccgcatcgag
     6481 gaagcgtcga gcaccgagcg cgaagttgac ctctatcgag taggcagcct gacggctgcg
     6541 gcttgtcatg gtctgcaa
//
LOCUS       DAJTNP010000162         6542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTNP010000162 DAJTNP010000000
VERSION     DAJTNP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(70..897)
                     /locus_tag="OO160_003825"
                     /pseudo
     CDS             complement(70..897)
                     /locus_tag="OO160_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
     gene            complement(976..1929)
                     /gene="lsrR"
                     /locus_tag="OO160_003826"
     CDS             complement(976..1929)
                     /gene="lsrR"
                     /locus_tag="OO160_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5525766.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2178..3713
                     /gene="lsrA"
                     /locus_tag="OO160_003827"
     CDS             2178..3713
                     /gene="lsrA"
                     /locus_tag="OO160_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5525767.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3707..4735
                     /gene="lsrC"
                     /locus_tag="OO160_003828"
     CDS             3707..4735
                     /gene="lsrC"
                     /locus_tag="OO160_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5525768.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4735..5727
                     /gene="lsrD"
                     /locus_tag="OO160_003829"
     CDS             4735..5727
                     /gene="lsrD"
                     /locus_tag="OO160_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5525769.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5739..6542
                     /gene="lsrB"
                     /locus_tag="OO160_003830"
                     /pseudo
     CDS             5739..6542
                     /gene="lsrB"
                     /locus_tag="OO160_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1477 a         1683 c         1724 g         1658 t
ORIGIN      
        1 cggcatacgg aacggagtaa tcggcaactg atgccgggcc tcgctgagcg tgcactgctt
       61 attatccgtg aacgtgccgc caagaaccgc ggtttgtgcc ggacgcacaa cgcctaaccc
      121 aaggcaacca agctgcacgt cgcctcctcc aacgaccacc agagtgcccg ccttcagacc
      181 gcagagttcc gccgcttgtg aacttaccac gcccagcaat gtgccggttt ctttgacagg
      241 agaaagaata tcggcacgca ggccagccat atccagcaat gcaggtttcc aggcacgggt
      301 ggttagatca agaagtcccg tggtgccagc gttagaggga tccaccgcca gttcgccgct
      361 gagcatatag gccagccagt cgctgatcat ggtgatggtt gatgcctgac ggtaaatatc
      421 ggaacgatgg tgcgccagcc aaagtaatct ggggatggca cttaaagcca gtgtttgtcc
      481 ggtcgctcga taaacttcgt tttcaaaggt attgttgtgc agttctttaa gttcgctaac
      541 ttcgcgtgcc gctctggcat ccacattggc gcaggcccag atcggtgttc cttcattgtt
      601 atataaaaca atgccttcac gcatagaaca tgccgaaacg gcagcgatat actccgggtc
      661 tatgccggcg ttgtgcagcg cctggcgcat acactcacac gccagttgcc agtttttgtt
      721 gagatcaaat tccatagaac caggaacgtc cggtactgcc agatgccgcc actccgcctg
      781 tcccactgct atttgattgc cttccaggtc gaatatcaca gcccgaatac ttccggtgcc
      841 tgcatccagc gccatcaggt agtactttga ttctgaaggg gtaaagagtc gagccattag
      901 attatcctcg gcttatgcgt tctgtctgtt cctctatacg ttctccatca ttcccggtaa
      961 taaggtcatg caaatttaac tacgtaaaat cgccgctgct gtgtcctgat cggtaaccag
     1021 tgcgttgata taaccgcctt tcattgcagc ggcaattgct tcagctttat tttctccccc
     1081 tgccacgcca acccggacgg gtatggtctt cagcgcgctt aaaggtaagc caatcagttc
     1141 gttatgtatt ttgatatccg tgacaacgtc accttttgca tcaaaaaagt agcctaaaat
     1201 gtcgccaacc gccccttttc ggccaatcat taactgttcg ccctggctga tataaccgga
     1261 gcgaatgatt gtcgcgtcgt cttgttgact cacggctcca atgccgacaa tcgccacatc
     1321 cgctgcttgc gcggctagca gaacatcttt gacgcaattt tcatttttta gcgtacgggc
     1381 aatgtcagcg gaggatgccc gcaacggagc cggaataata ttcacactgc acgccgcgtt
     1441 aagctgcccg atacccgtca tataagaacc gacgccaccg gagagcgtga ccaggcgaat
     1501 ttgctgtgac gaaataaaac cacttaagcg ttgcagcgta ttcatggttg cctcgccaaa
     1561 accaatcgcc agcatctgtt gtggttgaag taaactcatc aacatatgcg ccgcgcctat
     1621 ccctagtcgc ccgccgacat cagcatccgc aagcccaggg atcacccgga catgttgcag
     1681 cgaaaactga cgacgtaatt gagtttcata ttccagacag ccttcaaagc gagaattaat
     1741 ctgtacgcga ataatgccgg actgatgccc tttctccagc aagcgcgaca ctttcaaacg
     1801 ggtcaggccg agacgatcgc tgatctcgct ctgggtcagc ccgtcgtgat agtaaaacca
     1861 cgcgatccgc gcgacctgtt cttcttcaca cattccctgt tctgaaattg ccgaatcgtt
     1921 gattgtcata attcattctt cactttgaac atatttaaat ctttaatgca attgttcagt
     1981 tcttgcgcat ttatatctgt gatggcaacc acagtttgac tctacgagca tgaacaaacg
     2041 caaccgtgaa aatcaaaata gcataaattg tgatctattc gtcggaaata tgtgcaatgt
     2101 ccacctaagg ttatgaacaa attaaaagta gaaatacatt tgttcaaaac tcacctgcaa
     2161 aactgaacgg gggaaatatg caaacgagtg atacccgcgc gttaccgcta ctttgcgccc
     2221 gctcggttta taaacagtat tcaggggtca atgtcctgaa aggcatcgat tttacgttgc
     2281 atcaggggga ggtccacgcc ctgctcggcg gcaatggtgc cggtaaatcg acgttaatga
     2341 agattattgc cggtattacc cctgctgata gcggtacgct ggagattggg ggcaacaact
     2401 acgccagatt aacgccagtt catgctcatc agctgggcat ttatctcgtt ccccaggaac
     2461 cgctgctttt cccaagcctg tcgataaaag aaaacatcct gtttgggctg gcaaaaaaac
     2521 agctctccat gcagaaaatg aagaacttgc tggcggcgct gggctgccag tttgatctgc
     2581 atagtctggc aggatcgctg gatgtcgccg atcgccaaat ggtggaaatc ctccgcgggc
     2641 tgatgcgcga ctcgcggatt ctgatcctcg atgaacctac cgcctcgctt acccctgcgg
     2701 aaaccgaacg cttgtttagt cgcttgcaag agctgcttgc tactggcgtg ggtattgttt
     2761 ttatctcgca taagctgccg gaaattcgcc agattgccga tcgaattagc gtgatgcgcg
     2821 acggaaccat cgccttaagc ggcaaaacca gcgaactgtc taccgacgac attattcagg
     2881 ccatcacccc agcggtacgg gaaaaatcgc tctctgccag ccaaaaatta tggctggagt
     2941 tacctggtaa ccgcccacaa catgccgtcg gaacgccggt gctgacactg gaaaatctga
     3001 ctggcgaagg cttcaggaat gtcagcctga cgctcaatgc cggagaaatt ctgggcctgg
     3061 ctgggctggt gggagccgga cgcacagaac tggccgagac gctctatggt ctgcgtactt
     3121 tgcgtggcgg acgcattatg ctgaatggta aagagatcaa taaattatcc actggagaac
     3181 gtttactgcg cggtctggtt tatctgccgg aagatcgcca gtcatccgga ctgaatctcg
     3241 atgcttcgct ggcctggaac gtctgcgccc ttactcataa ccttcgtgga ttctgggcga
     3301 aaaccgcgaa agataatgcc accctggaac gttatcgtcg ggcgctgaat attaaattta
     3361 accaaccgga acaagctgca cggacattat ccggtagcaa ccagcaaaaa atcctcattg
     3421 ccaaatgcct ggaagcttcg ccgcaagcat tgattgtcga tgagccgacg cgcggcgtgg
     3481 atgtctcggc ccgtaatgat atctaccagc tgttgcgcag catcgccgca cagaatgtgg
     3541 ctgtgctgtt tatctcctct gatatggaag agatcgaact gatggcagat cgtgtgtatg
     3601 tgatgcatca gggcgaaatt acccactctg cactgaccgg gcgcgatatt aatgtcgaga
     3661 ctattatgcg cgttgccttc ggcgatagtc agcgtcagga ggcatcatgc tgaagtttat
     3721 tcagaacaac cgtgaaatca cggcattgct ggcggtggtg ctgctgtttg tattacccgg
     3781 ttttctcgac cgccagtatt taagtgtgca aacgctgacc atggtttata gcagcgcgca
     3841 aatcctgatc ctgctggcaa tgggcgcgac gctggtaatg cttacgcgca atattgatgt
     3901 ttcagtgggt tcgattaccg gaatgtgcgc ggtgctgttg gggatgttac tgaacgcagg
     3961 atattcacta cctgttgcct gtgtcgcgac tttactgctt ggtttgctcg cgggattttt
     4021 caacggtgcc ctggtcgcgt ggctaaagat ccctgccatt gttgccaccc ttggcacgtt
     4081 agggttgtac agaggcatca tgttgctgtg gactggcggt aaatggattg aagggttacc
     4141 cgccgaactg aaacagctct ccgccccgct gctgcttggc atttcagcaa ttggttggtt
     4201 gacgataatt ctggtggcat ttatggcctg gctgctggcg aagacggcgt ttggacgcag
     4261 tttttatgcc acgggcgata atttacaggg cgctcgtcaa ctgggcgttc gtacggaagc
     4321 cattcgcatt gtggcatttt cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt
     4381 tgcttcgcag attggtttta tcctcaacca gaccggtacc gggctggaga tgaaagcaat
     4441 tgcagcctgc gtgctgggcg gcattagttt gctcggtggt tccggtgcga tcatcggtgc
     4501 ggtactcggc gcatggttcc tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc
     4561 ggcatggtgg aatgatttta tcgcgggtct ggttctgctg gcggtgctgg tgtttgatgg
     4621 acgcctgcgt tgtgcgctgg aacgtaatct acggcggcaa aaatatgccc gctttatgac
     4681 gccaccgcca tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggccg cataatgcgt
     4741 attcgctacg gttgggaact ggctcttgcc gcactgctcg ttattgagat tgtcgcattt
     4801 ggtgcaatta acccgcgaat gttagatctc aatatgttgc tgttcagcac cagtgacttt
     4861 atctgcattg gcattgtcgc tctaccgctg acgatggtga ttgtcagtgg cgggatcgat
     4921 atttcgtttg gttcgaccat cggcctctgc gccattgcat tgggcgtact gtttcaaagt
     4981 ggtgtgccga tgccgctggc gatactcctg accttactgc tcggcgcatt gtgcgggctg
     5041 atcaacgccg gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg
     5101 ctgtatctgt ttgccggaag cgctctgctg ctttccggta tggccggagc gacggggtac
     5161 gaaggtattg gtggattccc gatggcgttt acagatttcg ctaacctgga tgtgctggga
     5221 ctccccgttc cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat
     5281 aaaacccatg ccggacgtaa tgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat
     5341 agcgcgattc cagttaaccg caccttatgt gcgctctatg ccatgacggg gctggcgtct
     5401 gcggtcgccg ctgtgctgct ggtatcgtat tttggttcag cacgttccga tctcggtgcg
     5461 tcgtttctga tgcccgccat caccgccgtg gtgcttggcg gggccaatat ttatggtggt
     5521 tccggttcca ttatcggcac cgccattgcg gttttattag tgggatattt gcagcaaggt
     5581 ttgcaaatgg caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctgcttatc
     5641 gtcgttgtcg taggtcgttc cgttagcctg catcgccagc aaattaaaga gtggctggcg
     5701 cgtcgggcca ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga
     5761 aaatcgcctt acttagcgct cttggcattg ccgcaatctc tatgaatgtg caggccgcag
     5821 agcgtattgc atttattccc aaactggttg gcgtgggatt ttttaccagc ggtggcaacg
     5881 gcgcacaaca agcgggtaaa gagctgagcg ttgatgtgac ctacgacggg ccgacagaac
     5941 ccagtgtttc tggtcaggta cagttgatta ataacttcgt caatcaaggt tataacgcca
     6001 ttattcgttt ctgcggtttc gcctgatggc ttgtgtccgg cactgaaacg cgccatgcaa
     6061 cgtggtgtga gagtgctgac ctgggactct gatactaaac cggagtgccg ctcttactac
     6121 attaatcagg gaacgcccgc ccagttggga ggtatgttgg tggatatggc ggcgcgtcag
     6181 gtgaataaag acaaagccaa agtcgcgttt ttctactcaa gccccaccgt tacggaccaa
     6241 aaccagtggg tgaaagaagc gaaagcgaaa atcgccaaag agcatccagg ctgggaaatt
     6301 gtcactacgc agtttggcta taacgatgcc actaaatcgt tacaaaccgc agaaggaata
     6361 ttaaaagcgt atagcgatct cgacgccatt atcgcccccg atgccaacgc cctgcccgct
     6421 gccgcacaag ccgcagaaaa cctgaaaaat gacaaagtag cgattgtcgg attcagtacg
     6481 ccaaatgtga tgcgcccgta tgtagagcgc ggcacggtga aagaatttgg actgtgggat
     6541 gt
//
LOCUS       DAJTNP010000163         6529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTNP010000163 DAJTNP010000000
VERSION     DAJTNP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            230..2662
                     /locus_tag="OO160_003831"
     CDS             230..2662
                     /locus_tag="OO160_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5525770.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2729..4198
                     /locus_tag="OO160_003832"
     CDS             2729..4198
                     /locus_tag="OO160_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5525771.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4198..5859
                     /locus_tag="OO160_003833"
     CDS             4198..5859
                     /locus_tag="OO160_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5525772.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6080..6421
                     /gene="symE"
                     /locus_tag="OO160_003834"
     CDS             6080..6421
                     /gene="symE"
                     /locus_tag="OO160_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5525773.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT         1932 a         1541 c         1506 g         1550 t
ORIGIN      
        1 gaaagaaacg caactataag atttttatat attttgttag agttgatatg gttcctgtaa
       61 cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata acagagatat
      121 caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat agccaggcta
      181 ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca tggcggaact
      241 gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct
      301 caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa
      361 agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct
      421 gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat
      481 tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt
      541 tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct
      601 tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg
      661 cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact attacgacga
      721 tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa ccattgaagc
      781 cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta
      841 taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt
      901 ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg
      961 tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc
     1021 gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc
     1081 agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag
     1141 cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct taaccgccac
     1201 gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta
     1261 ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg tccgtgttga
     1321 tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga
     1381 agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg tgatcgacga
     1441 acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca atccgatgga
     1501 taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt
     1561 taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga
     1621 tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt
     1681 tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct gcaaactggt
     1741 agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg gtcgtggtac
     1801 acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac
     1861 cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc
     1921 agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca
     1981 tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaccga
     2041 taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca agtttcacgt
     2101 caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg ccgacggtaa
     2161 actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc
     2221 gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga tcattcacga
     2281 gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga
     2341 cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg
     2401 cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt
     2461 gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca
     2521 ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg
     2581 atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca
     2641 attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca
     2701 gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca
     2761 agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc
     2821 ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga
     2881 taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg ccaacgcgca
     2941 gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc cggcgttgaa
     3001 aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag
     3061 cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca acaagctgaa
     3121 cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat
     3181 ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac
     3241 tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg
     3301 cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag
     3361 cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa aacagcttcc
     3421 gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg tacaaattcg
     3481 tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg atgtcattgt
     3541 taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga
     3601 gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag tactggcgaa
     3661 aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag gtgttaaaac
     3721 caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc gtttaccgaa
     3781 tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca
     3841 gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga aaaactacag
     3901 caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga
     3961 ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga
     4021 cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag
     4081 ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga tccagacgct
     4141 gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg
     4201 agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt
     4261 tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa gaaattgcgt
     4321 gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa
     4381 ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc
     4441 aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc gtttgaattg
     4501 ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc tgagtcatct
     4561 agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat cgagcaaagc
     4621 caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt aaataatcca
     4681 gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga ttttgttgtc
     4741 ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt ctttttttgg
     4801 cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa agtattaaat
     4861 agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga aaaagttagc
     4921 tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct ggacgcacat
     4981 cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac cgcggaactg
     5041 gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc
     5101 gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag
     5161 gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa
     5221 ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa
     5281 aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat agttaatatt
     5341 aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc acctgatatt
     5401 attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc aggagtcaaa
     5461 gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg cccgtcatta
     5521 tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta tccattagac
     5581 tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt tcttatgcaa
     5641 gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag tttttacaat
     5701 attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa gataaattct
     5761 attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa aacccaactg
     5821 catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc tcacaaaacc
     5881 aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt cacgctaatt
     5941 ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga tgatgtatcc
     6001 aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc acaatcacta
     6061 tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt tagaagcaga
     6121 agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt acccggatta
     6181 cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac
     6241 cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaacccgc
     6301 cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg
     6361 taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga aagtcgcctg
     6421 atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta taccaacgcg
     6481 ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaag
//
LOCUS       DAJTNP010000164         6435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTNP010000164 DAJTNP010000000
VERSION     DAJTNP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(150..511)
                     /locus_tag="OO160_003835"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="OO160_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="OO160_003836"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="OO160_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="OO160_003837"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="OO160_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OO160_003838"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OO160_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5525774.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="OO160_003839"
     CDS             complement(2779..3093)
                     /locus_tag="OO160_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525775.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="OO160_003840"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="OO160_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OO160_003841"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OO160_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5525776.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="OO160_003842"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="OO160_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="OO160_003843"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="OO160_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5760..5919)
                     /locus_tag="OO160_003844"
                     /pseudo
     CDS             complement(5760..5919)
                     /locus_tag="OO160_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5923..6435)
                     /locus_tag="OO160_003845"
                     /pseudo
     CDS             complement(5923..6435)
                     /locus_tag="OO160_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1722 a         1438 c         1316 g         1959 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tatcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat
     5341 taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac
     5401 ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt
     5461 gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact
     5521 attgttctta ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca
     5581 tactgaaatc cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc
     5641 taattaagtt gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc
     5701 agacacatat aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg
     5761 aggctctggt tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag
     5821 cgattgtaat cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata
     5881 atagtgttct ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat
     5941 ccagtcatac agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg
     6001 ttctgctgaa catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag
     6061 ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac
     6121 atgagccata catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc
     6181 gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc
     6241 tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg
     6301 gtcatcacga acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg
     6361 gaccggaacg gggatatcat cggcatggac tttaccgggc atcagcacat actggcgcag
     6421 gatgtcatac agcgg
//
LOCUS       DAJTNP010000165         6364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTNP010000165 DAJTNP010000000
VERSION     DAJTNP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..368
                     /locus_tag="OO160_003846"
     CDS             <1..368
                     /locus_tag="OO160_003846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525777.1"
                     /translation="LLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPVKITR
                     KSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRI
                     KRTGIYSIKGVNIKEYNVR"
     gene            complement(444..908)
                     /locus_tag="OO160_003847"
     CDS             complement(444..908)
                     /locus_tag="OO160_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5525778.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(905..1780)
                     /gene="nanK"
                     /locus_tag="OO160_003848"
     CDS             complement(905..1780)
                     /gene="nanK"
                     /locus_tag="OO160_003848"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5525779.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1777..2466)
                     /locus_tag="OO160_003849"
     CDS             complement(1777..2466)
                     /locus_tag="OO160_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5525780.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2514..4004)
                     /gene="nanT"
                     /locus_tag="OO160_003850"
     CDS             complement(2514..4004)
                     /gene="nanT"
                     /locus_tag="OO160_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5525781.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4114..5007)
                     /gene="nanA"
                     /locus_tag="OO160_003851"
     CDS             complement(4114..5007)
                     /gene="nanA"
                     /locus_tag="OO160_003851"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5525782.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5129..5920)
                     /gene="nanR"
                     /locus_tag="OO160_003852"
     CDS             complement(5129..5920)
                     /gene="nanR"
                     /locus_tag="OO160_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5525783.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1615 a         1714 c         1578 g         1457 t
ORIGIN      
        1 ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc tcaaaagttg
       61 ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg ttgtatccag
      121 taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca ttcggcttaa
      181 ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg ctggtgattg
      241 atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat aaaaagggca
      301 agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa gaatacaacg
      361 tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca aagatgggtc
      421 aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt caccaccacc
      481 tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc tcctggcata
      541 aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat ggcgctacaa
      601 agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc tgccatgcca
      661 aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg ctcgtgcaat
      721 tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaaaatattg
      781 tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc taaggccagc
      841 gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc
      901 atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcatggc
      961 ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacattt
     1021 ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc acgcactggc
     1081 aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg gagcgatgaa
     1141 tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt ctcgcatccg
     1201 cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca attgcttcca
     1261 cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc gtatgcccga
     1321 tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc
     1381 cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc agcgcctgat
     1441 actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa ttggtaagtt
     1501 gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc ggattcagcg
     1561 ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag
     1621 cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg
     1681 ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg
     1741 caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct ttttcatcgc
     1801 tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc
     1861 ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac gcccttcggc
     1921 aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg gctcttctgg
     1981 cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg
     2041 gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatgatg
     2101 gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc
     2161 gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg ccgtgatgcg
     2221 taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc tcaccaccgc
     2281 acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc ccgcctgctc
     2341 tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt caggaaccgg
     2401 ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa
     2461 cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca ggattaactt
     2521 ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct aaatggtttg
     2581 ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg aacgcgggaa
     2641 ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga gagcgatgcc
     2701 agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg
     2761 ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg gtcggtatcg
     2821 aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg
     2881 aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa taccggaata
     2941 atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc cagccagtca
     3001 ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa gaacagcaca
     3061 ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg cagcagcgcc
     3121 tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgcccgtt
     3181 ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc gcataacagc
     3241 ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa ccacagcgca
     3301 gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata
     3361 tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc ttccgcttcc
     3421 gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc
     3481 gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac gacggccccc
     3541 acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt tggccagctt
     3601 tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga
     3661 cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt cccggccgag
     3721 aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg
     3781 agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc
     3841 accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat taaaacgaaa
     3901 tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca ttgtgcacgg
     3961 ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg
     4021 tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt ttatttcccc
     4081 tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca tcaactgctg
     4141 ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg gtttgcggca
     4201 cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc ggaatacgcc
     4261 cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct gcgcggtctg
     4321 gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca tgatgttgta
     4381 agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga tttcgtcgta
     4441 accgttatag agcaccagat caggatgttc acgacggatc tgttccatct gatagagatc
     4501 gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt tgatctgatc
     4561 cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg gcaaaccatc
     4621 cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga aaggatagta
     4681 gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg ccgcaagttg
     4741 ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct tacctttcgc
     4801 ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta caaaggcttc
     4861 gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga actgaaccag
     4921 gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca ggagtgcagc
     4981 cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg tctgataaac
     5041 gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac agaaagctta
     5101 taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc tgaccgaaag
     5161 cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg gcttcgtcag
     5221 gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta acgttgttat
     5281 gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag tcgagcaggg
     5341 caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg cggtggaaat
     5401 caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg atttccagtg
     5461 cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag cgcaccaggc
     5521 tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga tgagaaagga
     5581 aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt cgagagacac
     5641 gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct gccagcgctt
     5701 cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca gacggtaatt
     5761 gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct tccaccattt
     5821 cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt gcaggtgaag
     5881 aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt cagtgtgatt
     5941 aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc aggtttttct
     6001 ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa aaattaatgt
     6061 aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag cattatgaat
     6121 attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga aaaatcacct
     6181 cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa atataacatc
     6241 attgttttca atctgccatt tatgggattg accgttttct tttgacacgg agttcaacaa
     6301 tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg tgcttgcgtg
     6361 gctt
//
LOCUS       DAJTNP010000166         6257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTNP010000166 DAJTNP010000000
VERSION     DAJTNP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO160_003853"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO160_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5525784.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO160_003854"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO160_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5525785.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO160_003855"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO160_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO160_003856"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO160_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1458 c         1477 g         1663 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgatgt
//
LOCUS       DAJTNP010000167         6151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTNP010000167 DAJTNP010000000
VERSION     DAJTNP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..171
                     /locus_tag="OO160_003857"
     CDS             <1..171
                     /locus_tag="OO160_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5525786.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO160_003858"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO160_003858"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO160_003859"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO160_003859"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5525787.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO160_003860"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO160_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5525788.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO160_003861"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO160_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5525789.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6151
                     /locus_tag="OO160_003862"
                     /pseudo
     CDS             5430..6151
                     /locus_tag="OO160_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1576 a         1309 c         1557 g         1709 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcatg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac g
//
LOCUS       DAJTNP010000168         6134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTNP010000168 DAJTNP010000000
VERSION     DAJTNP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1..393)
                     /locus_tag="OO160_003863"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO160_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO160_003864"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO160_003864"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5525790.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO160_003865"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO160_003865"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5525791.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..6134
                     /locus_tag="OO160_003866"
                     /pseudo
     CDS             5797..6134
                     /locus_tag="OO160_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    6077..>6134
                     /locus_tag="OO160_003866"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1878 a         1397 c         1057 g         1802 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
     6001 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
     6061 tccttcgcct ggcttccgct tattttgcaa aggcggagtt cgaccgcctc tggaaaaaat
     6121 aatgccactg ctgg
//
LOCUS       DAJTNP010000169         6057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTNP010000169 DAJTNP010000000
VERSION     DAJTNP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            433..1341
                     /locus_tag="OO160_003867"
     CDS             433..1341
                     /locus_tag="OO160_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5525792.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO160_003868"
     CDS             complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO160_003868"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5525793.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3656..4414
                     /gene="loiP"
                     /locus_tag="OO160_003869"
                     /gene_synonym="yggG"
     CDS             3656..4414
                     /gene="loiP"
                     /locus_tag="OO160_003869"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5525794.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OO160_003870"
     CDS             complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OO160_003870"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5525795.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5676..>6057)
                     /locus_tag="OO160_003871"
     CDS             complement(5676..>6057)
                     /locus_tag="OO160_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525796.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1577 c         1562 g         1427 t
ORIGIN      
        1 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
       61 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
      121 atgaaagcaa ttgggtacgc gagaagaaaa accccacaaa ccaaaataat aaaaccttaa
      181 ataacaacaa tatgaaattg atatttttaa agtcaacttt gtatttgtta tgttcatcac
      241 aaaatcaaca ttttgtttgt tattgtattt gtgatgcata tctctattta tttattgaat
      301 gttcgaaaac atacttttct aatgcaatgt tttttcgcta taccccttta tagattctga
      361 attctcacct gtcggctgtt ttctcctcat ctcatgtgac atattcccat actttactca
      421 aggaagattg tcatgaaagc acctgtttca ttaacctcat ttcgcccaca aaaatctctt
      481 gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt
      541 aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg
      601 gggctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag
      661 gctgtggtct atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca
      721 gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac cacctacaac
      781 actattgaag cattaaaaga tcgtctaatt gtccgtcagt tgccaactca gctggagaac
      841 attttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaatt ggttcaggat
      901 ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg taattgacgg ggtccagatt
      961 gaaaatattg atttttctga cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa
     1021 gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg
     1081 gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagcggaa
     1141 actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa
     1201 gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata atcccggact tgtagccctc
     1261 accactgccg aacgttggga cggtaaactg cccgatacca tgatccccgg cagcacagtt
     1321 ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gtccttctca caaaacgtct
     1381 tactgattac agcagcgctt ttgctttcgc aacaacgtta tcaacagtga agccgaactc
     1441 ttcaaacagc agctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcgcc
     1501 gttcaggcca acatacttgt accagtagtc agcaataccc gcttctaccg caacgcgtgc
     1561 agtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt caaatgcgtc
     1621 ggtagacggc atggacacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc
     1681 agcaaccgcc agttcaactt cagaaccggt ggcgatgaag atcagttccg gctgaccagc
     1741 acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc
     1801 ctgctgcgcc aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac
     1861 gccgtatttc cacgcgaccg cggattcaac ctggtcacac ggacgccatg tagacatgtt
     1921 cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc catcttcgcc
     1981 cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc tgtttcatca gcgcagccat
     2041 acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt acggcaggaa
     2101 gccaccgtgc agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta
     2161 gtggatgtag ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag
     2221 gttagacggt gccaggtcag cagagccgcc gaggaattca ggcaacagcg ggccgaacgc
     2281 ttcgatagca ttctgcgacg ctttacggct ggcgattttc gccggattag cctgcagttt
     2341 agcgataaac tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg
     2401 ggtaaattca gccgcttcct gcggataagc tttcgcgtaa gccgcaaact tctcattcca
     2461 tgcagattct ttcgcctggc ctgcttcttt cgcatcccac tgagcataga tttcagacgg
     2521 gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc
     2581 gcccagcggc gcaccgtggg agtcgtgagt accggctttg ttcggggaac cgaaaccgat
     2641 gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttcaactgc
     2701 gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc
     2761 ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca acgtgaccat cgatagaaat
     2821 accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca
     2881 aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg
     2941 gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc
     3001 cataccgact gcgttggcaa taccctgacc cagcggaccg gtggtggttt ccacaccagc
     3061 ggtataacct acttccggat ggcccggagt tttagagtgc agctgacgga agtttttcag
     3121 ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc
     3181 gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg
     3241 tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg
     3301 gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag
     3361 ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac
     3421 ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc
     3481 gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg
     3541 ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt
     3601 cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa
     3661 aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt gccagaatat
     3721 ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga
     3781 tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca aggcgacgat
     3841 tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg cgctaggcaa
     3901 caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt
     3961 tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga tgacagataa
     4021 cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa
     4081 agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg
     4141 gattgtcggc agtttatctc aatcacaact tggtgatctg ggcgagaaat tagtcaattc
     4201 gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc ttctgcacca
     4261 acgcggcatc agcccggcag gtcttgccac cagctttgaa aaactggcga agctggaaga
     4321 aggtcgtcaa agctcaatgt ttgacgacca tcctgcatcc gccgaacgtg cccagcatat
     4381 tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta gtggtgtcga acgtaggtca
     4441 gataaggcgt tcacgccgca tctgacatta atggcacatt tgttaccttg tgcgcattgc
     4501 cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg tcggataagg
     4561 cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc atctggtgct
     4621 tactcgccct ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc
     4681 agagcggtga tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg
     4741 atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt caggccgcca
     4801 atcactggcg taccggttcc cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc
     4861 aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgctg
     4921 cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaactcggta
     4981 cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag
     5041 aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag
     5101 tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca
     5161 aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc
     5221 gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag atcgccgcag
     5281 tccacgacgt tcagacgctc acgcatgtcg aaattccacg ggaagcggtt gtgttcccag
     5341 gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc cgcacgacca
     5401 gaagtggcca tatcgaacgg cacgccagta atcacccagt ctgcatcgct gtcatacggc
     5461 tggaagttca tcggcaggcg taaaaaacca aaggcattgg aaaccagtga gttatcgtat
     5521 tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt
     5581 ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt
     5641 atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca
     5701 tagccattgt tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc
     5761 gggccttgta attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc
     5821 gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt
     5881 ttatctttgc ggaatccttt aatcactaac gaatctccgg cattcacaat atagccgttg
     5941 ttattcagcg aaccttgctt gccgttcagc acatccaggc catcagccgt agcaacgatc
     6001 tcgtagttgg tgttacgact gtaattacgg acatataatt gataattcat tccgact
//
LOCUS       DAJTNP010000170         6034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTNP010000170 DAJTNP010000000
VERSION     DAJTNP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            520..1698
                     /gene="ltrA"
                     /locus_tag="OO160_003872"
     CDS             520..1698
                     /gene="ltrA"
                     /locus_tag="OO160_003872"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5525797.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1781..1960
                     /locus_tag="OO160_003873"
                     /pseudo
     CDS             1781..1960
                     /locus_tag="OO160_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1990..2472)
                     /locus_tag="OO160_003874"
                     /pseudo
     CDS             complement(1990..2472)
                     /locus_tag="OO160_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2728..3708)
                     /locus_tag="OO160_003875"
     CDS             complement(2728..3708)
                     /locus_tag="OO160_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5525798.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3708..4907)
                     /locus_tag="OO160_003876"
     CDS             complement(3708..4907)
                     /locus_tag="OO160_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5525799.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5229..5567)
                     /locus_tag="OO160_003877"
                     /pseudo
     CDS             complement(5229..5567)
                     /locus_tag="OO160_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5634..>6034)
                     /gene="istB"
                     /locus_tag="OO160_003878"
     CDS             complement(5634..>6034)
                     /gene="istB"
                     /locus_tag="OO160_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5525800.1"
                     /translation="RVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIID
                     EIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRIL
                     HHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1647 a         1382 c         1428 g         1577 t
ORIGIN      
        1 ataagttatc catgctctga ggtgggtaga ggtgggccct ccggtcaggg tttacaggag
       61 cacagaccaa acgacccggt gctgcttcat tctaaggaat gaggtggtga gcgactcggg
      121 aaaaggcaga gatctactgt cacgtgggtt cgggaagctg aacgaaagta aactgtccga
      181 tgaagcatcg aaatcttaga tgatgccaaa atggggccgt acagccgtcc cctgataagc
      241 gcagaagaca cctgcttact gactgcgcgg catccggtgt gaaggcagca ggatcccggg
      301 ccagaccatc tcagggaacg tgagaacctg tttccggcac gtatcgggaa acaggggcgg
      361 acccattcgt agtagtgatg aaagatctgt aatgggtctg gagcgaaggg atggggatca
      421 cattcattgc agaagacaca acccaaaagg ggatgacgta actgatgcaa cgaaaatcat
      481 ttgaaatacc aaaagcactg gtctgggcat catatctgga tgtgcgtcgc aacaagggag
      541 ccccccggat gcgacgggca gactttgaaa atgttcgacc agcaacgtga tggcaatcta
      601 tacaagatct ggaaccggct atgttcggga acatggtttc cgccaccagt gctggaaaaa
      661 cggatcccga agtcgaatgg caaggaacgc attctgggga tcccaacagt atccgatcga
      721 attgctcagg gagcgataaa acttttcatg gaagaaaagc ttgacccgat tttccatgct
      781 gattcatatg gttaccgacc aggcaaatca gctcatgacg ctctgaaaca atgtgccatc
      841 cggtgctggc gttatagctg gatactggaa gtggacatca gtgcgttttt cgaccatgtg
      901 agacatgatc tggtgctcaa agctctggaa caccacggga tgcctaaatg ggtcatcctg
      961 tactgtcgac gctggatgga agcgccaatg cagagttgtg aaaatggaga attaataacc
     1021 cgaacacgag gcactccgca gggcggggtc attagtccgc tacttgctaa cttgttcctt
     1081 cactatgcat ttgatttgtg gatggaaaga gaatatcggg gggtaccgtt tgagaggtac
     1141 gctgacgata ttgtagtaca ttgttcacga atgagtgatg caacaaggct aaagaacagg
     1201 ttatcggagc gcttctcgga agtcgggctg gtcctgaatg cagggaaaac gaatatcgcc
     1261 tacattgaca cgtttaaaag gagaaacgtc gcaacgagtt tcacctttct cggatatgac
     1321 ttcaaagtgc gtacgctgaa gaatttcaac ggcgaacggt accgaaaatg catgccgggt
     1381 gcgtcaaatg cagcaatgcg caaaataaca gaaacaatca agaagtggcg tatacatcgc
     1441 tcaacagctg agagtttgct ggattttgcg aggcgctaca atgcgatagt gagaggctgg
     1501 atcgagtact acggaaagtt ctggtccaga aatttcaact atcgactgtg gagtgcaatg
     1561 cagtcacgtc tgctcaagtg gatgcagtct aaatacagac tttcgaaccg gaaggctcag
     1621 cgaaagctga cgctggtaag gaaggagtat ccgaagctat ttgttcactg gtatttacta
     1681 cgtgcatcaa atgagtgatc aagagccgta tgacgcgaga gtgtcacgta cggttccgtg
     1741 agcagccggg gagtgaaatt ctctccggct gactcgactc gcgggttccg acagcgatga
     1801 cgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca atgtggaacc
     1861 agaaaaatgg ctgcgttacg tcattgagca tatccaggac tggccggcaa accgggtacg
     1921 tgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaatac ggttctgacg
     1981 agccgcttac ttttggcgta attctgctgc gcctttactg aacacaatgc cgtattcagc
     2041 aagaagtcca cgaatgtgat ttatcagttg tgtcctttcc attgaaacca accgacgcat
     2101 attcaaaaaa gtctggacgt cctgttgctc cagagttttt attggtacgg gtcgtatagt
     2161 tccatgttgg caggcctcag ctattgcctg tgcgtcgtta taatcatttt tctggcaccg
     2221 ctgataagct ttaacatgtt gtgccggaag caatattaca tcatggtcga gtttcctgat
     2281 ttcccgggcc cagtaatgag atgccccgcc agcctccatc gcaacagtca ttttaggctg
     2341 ttgcaccagc cagttaagca gttgattgcg agaaagtttg gtctttcctg cgggtttgcc
     2401 atgagcattg atactgaaaa gataaaagtt cgttttagcc agatcaatgc cggtgatttt
     2461 ctgttcagac atggtatccc gcttactttt gttgttaaag tttatccatc atggcgcaat
     2521 tgaaggctgc cgtgaaagag ggggaagact atctcatcgg ttactgttaa acggctaaga
     2581 gaaactatct ttttcaagca aagaagatac ggtgaaacag gcgatttcac cgtgaaatac
     2641 tgttcaaaaa aaacgctaat aaaagcaaaa tttcttttaa aacagccaat taaaaactgg
     2701 cgagttgctg gcgaaaaact tgaattttca cagcgaattc agattatctt ttagtacaag
     2761 agcaatcata cgatctaact tatcctgaac ttccaacggt aatctgccga attcatatga
     2821 gaacgtacgt ccctttactc gttttcgagc aaatttatct ttactatcaa atttccataa
     2881 caaagttaca accgctttat cctttactcc tgaatcaagc aatgaagaca tttccttggt
     2941 tataagccgc ataatactgt ttttaacctc gtcttcagaa agattgtcat cagcagtaat
     3001 aagctcaata ctcggcgaga tattcttcac aacttcatca atagatatat cacccagcct
     3061 cagtcgttca cttaattcag ccagttgcct gtaatcggcg tatgtcagct cagactgaac
     3121 aggaaaaaga gacacaaaat ctttaggtac ggacgctgct tggagggcac gagtcacctt
     3181 agctgccgac aatccctctc tttccgcaat ttgcttctgg ttcataccgg cctctttgag
     3241 acgtactaaa cgcaacccaa tttcacgaat attatgttca agtgacgtct gcaaatcttt
     3301 cgccagatgc tgagcttcag aaacagtaag ctcatcatcc gttacaagta cacgtaatcc
     3361 aaccttgcat agcaaagctg ctgcccgacg acgggaacca tccaaaatct caataagatc
     3421 gccagtccga atcccaatac atggatagaa ttgttgtaaa cgtatagttc tggtgatgtc
     3481 ttttagtgag tccggcgtca gggctgtctg ttcgcggcca ttcacctctt caaccacaaa
     3541 agtttgagta tccactttgt catgatcaac cttgatctca gtaaattttg cctttcggcc
     3601 agtattgagg gtaaatacat gaactggagc cgatatttct tcagtattat tcaggatatt
     3661 tgtattcagc gtacggccaa tagtcggtcg gtgctttctg ttttccatca gtagttggcc
     3721 cttataaatt caatacgatc aaaaactgct ttagcgaaat cttctgctgc catacgtgca
     3781 tttttcaaag cttcaccact gccaacataa gtaacaggat tcgcagatat cacggtatca
     3841 aacgactcgc cgcttctctc aaaaccatca agacgtggca ttgaaacatc aagcatgtca
     3901 ccaccgaaaa tctcttttgt caggctatgg cagtatttat gatctgactt attcgccagt
     3961 ttcgacatga agccaatatt tgcctgcaaa cggcatgaac atccatcctg ttcaataatc
     4021 tgtacaagtt caggtaaacg agccaaatat ttaagcgtgg aatggaaatc aacttgtgct
     4081 ggcggcaccg gtgtaaacat gatatctgcg gcagcaatgg catttttcaa aaatgcgtcc
     4141 agatgcgggc ctgtatcaat aaggatgaag tcgaaatcat gcttcagctt atcaataata
     4201 ttctcacgca atatcgcgtg tttgttttgt cctaatagat gctcctcgca cagagtatcc
     4261 cagtttgatg caataaaggc gtcgtcaata gaagccggca taacataaac gccagggata
     4321 actgacggaa cgataaaatc ttccagtaat tcctcacgac tcacgttctg caacatagcc
     4381 tgcggtgcgg ttgtatcaac cagccccacc gcatgcaagt agttcagaaa catagtggct
     4441 gatgactggg gatctaaatc aaggagcaga atacgtaaat cttctgacag caggtgagga
     4501 tgagcacgca gggcgtgagc tacggaaaca ctactcacgg ttttagacac accgcctttt
     4561 agactaccga tgaatatgga atatgcctca ctataacgat cgcgatattt aggaatgcca
     4621 cgatgtgcat agatatcgat aatgttctgg atggttaatg catattttgt cgcggttcct
     4681 gctgggcgtt tttcaaactg atagccctga gcctccatct caccgactgc accatccacg
     4741 cttgcgcgcg ttaattttgg aagcttagct acagcagact ttgaatagac ttgataaaaa
     4801 acatcagcct gaaactcctg cttttgctcc tgaatctgtt ttgttagggc gagcaacatt
     4861 ttatctgcac gctgcgcaac cttacttaac tgctcaaaac tagtcattcc ccctccgtgc
     4921 tatgtaggat tttaacttat tacaaaaaaa actgcattag cagggtaatg catgcaagta
     4981 catattgtac atgcttatat taaaaatgcg cgcattgctg atttattaat catcggaatg
     5041 aatattactg taagatagta tgcctttccg ggagaaagac gctgatttcc cccgaataat
     5101 ccagttcacc accacactcc ggacaacagc tttcttcagg ctccagatgg tgtatttcgc
     5161 gggaaacatg ttccggtaac ggacggcgat gacgtgactg tcgcaacagg cgggggtagt
     5221 gcttccaatc agtagaacat gtgcttttca atgaaggagc cgatgacttt ttcgtggatt
     5281 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg
     5341 gtccggttgt tacgtgcaat acgttgcgtg aagattttgc cggtcaggtg tttctccttc
     5401 ggcacttctc tggcgtagct gccccagtca tcgctggtca ccatgccgat acagaacgga
     5461 gtcaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc
     5521 aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgtgt caacgacgga
     5581 tgaaaagtga tccacttata tctccaccaa cggcccaata ttgatccacc gttttactca
     5641 ggattagctt ctgctataac cccggccttt cgtttctgtc tgagtcgata gctttctcct
     5701 ttgatttgaa cgacatgtga gtggtgtaag atacggccca gcatcgctga ggtcagtgct
     5761 gcatcaccgg cgaacgtttg atcccactgc ccgaacggta gattggatgt caggatcatt
     5821 gcgctctttt cgtaacgttt agcgatgacc tggaagaaca gttttgcttc ttcctgactg
     5881 aacggcagat agcctatttc atcaatgatg agcaggcggg gggccattac tccacgctga
     5941 agcgtcgttt tataacggcc ctgacgttgt gccgtagata actgaagtaa cagatctgct
     6001 gctgttgtga agcgaacttt gatacctaca cgga
//
LOCUS       DAJTNP010000171         5847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTNP010000171 DAJTNP010000000
VERSION     DAJTNP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..273
                     /locus_tag="OO160_003879"
     CDS             1..273
                     /locus_tag="OO160_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5525801.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(239..646)
                     /gene="nudG"
                     /locus_tag="OO160_003880"
     CDS             complement(239..646)
                     /gene="nudG"
                     /locus_tag="OO160_003880"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5525802.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            733..1353
                     /locus_tag="OO160_003881"
     CDS             733..1353
                     /locus_tag="OO160_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5525803.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1362..2669)
                     /gene="ynjE"
                     /locus_tag="OO160_003882"
                     /pseudo
     CDS             complement(1362..2669)
                     /gene="ynjE"
                     /locus_tag="OO160_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2736..3389)
                     /locus_tag="OO160_003883"
     CDS             complement(2736..3389)
                     /locus_tag="OO160_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5525804.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3389..4856)
                     /locus_tag="OO160_003884"
                     /pseudo
     CDS             complement(3389..4856)
                     /locus_tag="OO160_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(4829..5680)
                     /locus_tag="OO160_003885"
                     /pseudo
     CDS             complement(4829..5680)
                     /locus_tag="OO160_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1344 a         1592 c         1557 g         1354 t
ORIGIN      
        1 gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact ggctaacccg
       61 cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc aggcgggcag
      121 tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc tgaaggggcg
      181 gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac ccttagcact
      241 aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga atgtcagcag
      301 gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc tgatgttcat
      361 gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg ataatccggc
      421 ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg atgtccaatt
      481 cttcgtttaa ctcacgcacc agcgcctgct gctggctttc atccagctcg actttacctc
      541 cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg agtaaaattt
      601 tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat tacacacggg
      661 cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag gctttatatt
      721 taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt gttgcatcag
      781 tgcgtgcggg ttcttgataa accgggcatt acgccagatg gtttaacatt agtgggattt
      841 gccatcggcg tgctggctct gccgtttctg gcgctgggct ggtatctggc ggcgctagtc
      901 gtcattttgt tgaacaggct gcttgatggt ctggacggtg cgctggcgcg gcgcagaggg
      961 cttaccgacg cgggcggctt tcttgatatt tctctcgatt ttctctttta cgcgctggtg
     1021 ccgtttggct ttattcttgc tgcaccggag caaaatgcgc tggcaggcgg ctggttgttg
     1081 tttgcgttta tcggcacggg cagcagtttt ctcgcctttg ctgcactggc agcaaaacat
     1141 cagattgata accccggtta tgcacataag tcgttttact atctgggggg attaaccgaa
     1201 ggcacagaaa cgatcttact gtttgtgctg ggatgtttat tccctgcatg gtttgcgtgg
     1261 tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct ttacgcgggt gtggagtggg
     1321 tatctgacgc tgaagtcgct ccagcgtcag taattgcaat gttatttgct actgcccggg
     1381 ccgcgttcac cggttgcaac cggatttttt ggatcgctgc tccattcgta ccagccgccg
     1441 tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt acataaaggt ttcggacgcg
     1501 cgccagccgg tgccgcagta gaatgaaact tgctgatctg gtttgatatt ccatgctttc
     1561 cacatagcgg taatatcatc ggcgctacgc atggtgccat ccgggttata gaaatcttcc
     1621 atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc cggctatttc accttttggt
     1681 ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg gccacgaacg aatgctcacc
     1741 agcgatgcat cctggcgatg cagcagtcca cgggcctgtt ccatatcgag catcaactgc
     1801 ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt tcactttcgg tggcgttcct
     1861 cgctcaacag gcagtcccgc gtcggaccag gtttgccagc cgccatccag cagacgcaca
     1921 tctttcacgc ctgcgtagag cataatttgc gccacacgcg ctccagcgta tacgtcacgc
     1981 ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt ttgccagcat cgcttttagt
     2041 tgttcatcag aaactttgtt ccacagcggt tcgctttcca cttcgttggt atcgatgtag
     2101 tcagcgccag gaatatggct gataaggtaa agcttaggag cgccctaggc cgcttcaatg
     2161 actttccagt caccgacagg tttcgccgta acctctttac cttgttgcag gtcatgcagc
     2221 cattgcggat aaaccagctg ctcaaagtgc ggcagttttt gtagacggga aggttcgctt
     2281 agcgcgtcac tcagaatgga gatatgcgta aaacctgctt tttgcagtcg cgttttgacg
     2341 gcgtcaacat ctttgccatt accgtacagc gccaccgggg catcggcttt caggttatgt
     2401 tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt ccagccagct agcagagagg
     2461 tttaaggcgg caggttcatg accagaaggg ccatttaagg tttgtggcca gccgttataa
     2521 aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt gctgaagttg gtcaagtgta
     2581 agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag ccagccctaa agccattgcc
     2641 agcgcggtca tttgagaaac acgtttcatc gaaaatcccg acattgtagt tttgagtgcg
     2701 ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc gcagtttgtt gtaattttct
     2761 gaccactgcg ccatatccag aacagaacta tcagcaggaa catcctggag atcgtgcgtt
     2821 acctgaacga cggggatcgc cagggcgcga acttcgctga acacccactg gcgaaaatta
     2881 tcgcgcagag ccacatcaag acggctgaat ggctcatcca ggagtaacgc ttttggctgg
     2941 gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac cgccagacaa agtggcagga
     3001 tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg catcattcac ggcattacgt
     3061 cgggcattcc ccttaagtgt cgccggtagc gccagcagta aattttgccc gacgctgaac
     3121 tggtcaaata acagtgcatc ctgaaaaaga atgccaatct gacgctgtgc ggtgggtagc
     3181 atgtcaatcc gttgctcatt gagccatagc tcacctgtac aagaaaactg tccggccagt
     3241 gcaccaatca tccatgaaaa cagagtggat tttccacagc cagacggccc cattaacgtg
     3301 acgatgtcac ctttatcaac cgtaaagtta acgtttgtca gcaagcggct ttctggtaaa
     3361 cgtagcgaaa catttttcac gcagagcatt agcggattcc ttgtctgaca taacctaccc
     3421 attttgcgac taacgcggtc agggcaaaaa taataagcgg taacaacagt tgccataacg
     3481 cctgggcggc gagaataccg ttgctgccgc cgctgcttaa tgccaccgcc tcagtggtga
     3541 gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat atactgcgca atactgactg
     3601 aaaatcccac cgcgaaggca atcagcacag ggcgcaacat gagtgggcat ttcacgtaaa
     3661 agaagatttt ggcccgcgac cagcccagtg tttgcgcaat caaaattaac cgtgaatcaa
     3721 tgcgctgcca ggcgggttgc aggataaaca gcatccacgg cattacccac agcagatgcc
     3781 cccagaccac cgccgtccag cttccatcca ggttcagcca tagcgccagc gtgtactggc
     3841 ccgccaccag tggcagagcg ggcaatagaa tgggcaacca tagccacaac tggcgacgct
     3901 gtgcccccca ttccagccac agcagtaaca ggagcaaagc gatgaatgtt gctaccagcc
     3961 ccattgtcag actgttgatg agtgcttcac tattgatcgt cgactgatcc gcgaggatcg
     4021 ccagcagaac cacacacagc acaccggtta agggtaaaaa actcgccagc gtagtgcctg
     4081 gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg gggaatagtg cgccgccagc
     4141 tgcgccaaaa cgtaggcggc gagtaacagc atcagcagca gactggcgag tgcgcctttc
     4201 gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc taattactgc cagcgtcggt
     4261 ggattacctg gcccaagaat aatcgccaca tccacgaccg aaagcgacca ggcaacaatc
     4321 gccagcatcg ccattgccag cggctgtagc ccagcgaatc cagcacaatg acctgctcag
     4381 ccgtttttcg ctcaatactg ccactaagat ccacagcaga aatgcgcttt ctttcactgc
     4441 aagggtgagg cccagaccga tccaaatcgg tccattggcg gagtgaaata cgggaaatag
     4501 tcatatagca gccctccgtc agcaaagagc agcagggcgc tggtggcaaa agccacatgg
     4561 ggaatggcga gcagccacgg cagacgggcg cacatacgct gccatttcgg cccaggccat
     4621 agcgccacaa tcaccagcag ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac
     4681 accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt
     4741 gtcaacgaca acgcaggcga tatcagcgtg agagctgccg ggatcagcgg tgcataaatc
     4801 accgccacca tcgcccacag caaaaagatt aatgcgtacc gtaacggcgt aaccattctt
     4861 gttccagtgc attcacccat cctgcgtgcg gttcagccag taccggcggc agatcctgcg
     4921 gcattcttga ttgcaatatt tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag
     4981 aaggatcgcc ccagacaacg ggatctgctt tacgcagttg cgcatcgggt gagagcagga
     5041 aattggcaac taccttcgcc gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat
     5101 gcacgttgcc aatcatcccc tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc
     5161 cgctggcgat tttttgctgc gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc
     5221 cggctttcag cagagcatcc atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc
     5281 acaaatacgg atgcagtgca tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag
     5341 tcgcatcgtc cggcgcttct tttaatgcgg cgggatctgg cgtcagcata atcagcaact
     5401 gttcaagaaa cgccgtgccg gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat
     5461 tggctttagc aaactccagt aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc
     5521 ggcgggcgat aaacgtcagt tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca
     5581 ctgaaaaatc tccccgcacc ggcagctgtg tgtcgacata gcgccagttg ggcagagtct
     5641 ccgcccagcc cgtttgtaat aaattggcct cttttaaggt gtaatgccga tcagttaagg
     5701 atcagttgac cgatccagtg gctgtgtaag aatccggaaa cgctcacttg tttccggatt
     5761 cttttatgca cattggacag gctcttgatc tggtatcccg ttacgattct ctgcgtaacc
     5821 cactgacttc tctgggggat tacctcg
//
LOCUS       DAJTNP010000172         5692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTNP010000172 DAJTNP010000000
VERSION     DAJTNP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..170)
                     /locus_tag="OO160_003886"
     CDS             complement(<1..170)
                     /locus_tag="OO160_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525805.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO160_003887"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO160_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5525806.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO160_003888"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO160_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO160_003889"
     CDS             complement(3616..4383)
                     /locus_tag="OO160_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5525807.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO160_003890"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OO160_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5692
                     /locus_tag="OO160_003891"
     CDS             5484..>5692
                     /locus_tag="OO160_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5525808.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALS"
BASE COUNT         1407 a         1467 c         1478 g         1340 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tg
//
LOCUS       DAJTNP010000173         5657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTNP010000173 DAJTNP010000000
VERSION     DAJTNP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..433
                     /gene="mddA"
                     /locus_tag="OO160_003892"
     CDS             <1..433
                     /gene="mddA"
                     /locus_tag="OO160_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5525809.1"
                     /translation="NDQTVDADNRIAWFEARTIAGYPVLVSEEDGVVTGYASFGDWRS
                     FDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHLH
                     QSLGFVVTAQMPQVGTKFGHWLDLTFMQLQLDERTEPDAIG"
     gene            430..879
                     /locus_tag="OO160_003893"
     CDS             430..879
                     /locus_tag="OO160_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5525810.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(880..1113)
                     /locus_tag="OO160_003894"
     CDS             complement(880..1113)
                     /locus_tag="OO160_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5525811.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1199..1372)
                     /gene="ortT"
                     /locus_tag="OO160_003895"
     CDS             complement(1199..1372)
                     /gene="ortT"
                     /locus_tag="OO160_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5525812.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1567..1662
                     /gene="yncL"
                     /locus_tag="OO160_003896"
     CDS             1567..1662
                     /gene="yncL"
                     /locus_tag="OO160_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5525813.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2064..2873)
                     /locus_tag="OO160_003897"
     CDS             complement(2064..2873)
                     /locus_tag="OO160_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5525814.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3083..4507)
                     /gene="patD"
                     /locus_tag="OO160_003898"
     CDS             complement(3083..4507)
                     /gene="patD"
                     /locus_tag="OO160_003898"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5525815.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4529..5323)
                     /locus_tag="OO160_003899"
     CDS             complement(4529..5323)
                     /locus_tag="OO160_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5525816.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5313..>5657)
                     /locus_tag="OO160_003900"
     CDS             complement(5313..>5657)
                     /locus_tag="OO160_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5525817.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1490 a         1439 c         1254 g         1474 t
ORIGIN      
        1 gaatgaccaa acggtggatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc
       61 aggttatccg gtgctggtaa gtgaagaaga tggcgtagtt acgggatatg cctcgtttgg
      121 cgactggcgt agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc
      181 cgatcatcag ggcaaaggtc tggggcgtaa attgttaagt cgattgattg atgaagcgcg
      241 ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cttcgctgca
      301 tctgcaccag tcgctgggat ttgtcgtcac cgcacaaatg ccgcaggtag gcactaaatt
      361 tggtcattgg ctggatctga catttatgca gttgcaactc gacgagcgca ctgaaccgga
      421 cgcgattgga tgaatcagtc gctcaccctt gcttttttga ttgccgccgg aattggtctg
      481 gtagtccaga acacgctgat ggcgcgcatc acccagacct cctctaccat tctcatcgcc
      541 atgttgctga actcactggt ggggattgtg ctctttgttt ctattttatg gtttaaacaa
      601 ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct ggtggacact tatacctggc
      661 ttgctgggtt cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg
      721 acaactattg cggtactggt cgccagccag ctgattggcg gtctggtgct ggatatcttc
      781 cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa tatgtggcgc aattttactt
      841 gtggttggcg cctggttggt ggccagacgc tcattttaat cagaggatgg tgccgccttt
      901 ggttaactgt tcgtggcgcg cttccatatc ttctttatgc ttgcgaccgt gatgagcaat
      961 agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc
     1021 gctcagggcg attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc
     1081 cacgcgcgcg atgacttcat ccagatgcga catactttct ccttagccac ttgtgaaatt
     1141 tatcgattaa tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt
     1201 aaaacagtga aaacaacagc gccaccggga aactcatcgg ccatgtggcc cccaccagaa
     1261 aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg
     1321 cgattgctgc cataaccgca taaaaaacaa gcatatgttg atagagaaac atgtacgggc
     1381 gtccagccat atgaataaaa cgcgcgcaaa tatgcgccat tttttgacgt gcatcaagtt
     1441 ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg cccttatcca tcttcagaat
     1501 gagtattatt gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg
     1561 gcaaaaatga atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt
     1621 ttgtttactc ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc
     1681 agcacgtact gtcagagaaa tcaactctct gacagtacgc aagatcttct catactctcc
     1741 aagtttgcaa cattatcctt aatatttact tctttttgct ggcatctaca ttgaaatgag
     1801 tagaaaacat ccccctactg tcgtctatac aaacagctta ctgattcaaa cggaatcttg
     1861 ttttcacatt tttctatcac gaatacttta tacgcgcggg agtctataaa tgctttatac
     1921 tttcaaggaa taacgtaaat ataaaaagat ggcgtaaagt atatgcatgg caggatgttt
     1981 gcaacctcct tgatgacgct gccccattga aattgcaaac cccgccctgg ttatcttgcc
     2041 catttatcat gcaaaacaga taattatagg cctctgaata cctcagaaag attgaatttt
     2101 ttatagtccc tctcttgaat atttccattt gatatcaatc gattcatctc ttcaacaaaa
     2161 taatttatat catcacactt attattatcc ggaagatctg agtagttata attccaccat
     2221 tttattctca acagttcatc aataacattt gaatcaaacc taaatctgat tatttttgca
     2281 ggaactcctg caactatggc atatgggggc acatctttgg taacaacgga attggcagca
     2341 ataacagcac catcacctat tgcgatgcca cctttcaata caacatcatt tcctatccag
     2401 acatcatttc ctataatcgg attttcaggt ttggtaatga atggtttcaa tgtccagcta
     2461 cgttcaaatt cactcatagc aaacttatca aattcaccgt tataagtcat catgtgagta
     2521 gtaaaacgat tcaaaggatg atttccaccc attattttta catttgatgc aatactacaa
     2581 aatcgaccta tacgtatatt ccctgaaaaa tggcaggtcg gaaatgaaaa agcgccgaca
     2641 ctggagaaat ttttcacagg cattgttgaa tattcttcag caataacatt atcttcaatt
     2701 gttatcttgt caccttttga gaaaagaaat aaattccttt ctttatacga cttcttcccc
     2761 ttatgattta aaaagatttt attatccata caaaactcaa agtgtttttt cgtccatctg
     2821 aaggtaaggg ttttattttt atcttccgtg aatttgtaca attgaacttt cattgttaat
     2881 acttctactt ataaagtatt agtttaataa cgatactcta aagccttatg ctaatcaact
     2941 gtgacaggat gaaaaatcgc catcaaacat ttagcctaat ttttacgcca gttattaatg
     3001 taatcattct aatacttaac cgccattaaa ctcagaacct aaaacgatag tggccgtcct
     3061 ataacagcca ctaacatcta acttaatgtt taaccatgac gtggcggacg acggtgtaat
     3121 cctccagccc ataaagtgac atatccttgc cgtaaccaga aagtttctgc ccaccgtgcg
     3181 gcatttcact taccagcatg aaatgggtat tgacccaggt acaaccatat tgcagccgtg
     3241 cgctgacgcg atgcgccctg cccacatctt tcgtccatac cgaagatgca agtccgtact
     3301 ggctgtcatt cgcccagttc accacctgtt cttcgttgtc gaagggcgta acactcacta
     3361 ctggaccaaa tacctctttt tgcacgatgg catcgtcctg taatgcgcca gccagcagcg
     3421 tcggcgcata gtaatagcca ttacccttgc gcttttcacc gccagtgatc actttgatgt
     3481 gccctgtcgc tttcgcctct tctactgcct tgctgacgcg ttcgagatgc gccagcgagc
     3541 ttaaaggtcc aagctccgta gactcgtcat ccggcgcacc agattttaac gttgccaccg
     3601 cagcacccag tttttccacc agcgtatcgt aaatgccttt ttgcgcgtag atccgacaag
     3661 ccgcagtaca atcctgtcca gcattgtaat agccaaatgt acgtacacct tcgaccactg
     3721 cttcaatatc cgcatcatca aaaacaatca ctggcgcttt gccaccaagt tccatatgag
     3781 tacgcttaat ggacgacgcg gtatggctga tgatgtgctc gccggtggcg atagagcccg
     3841 tcagcgacac catccgcact ttgggatgac cggtcagcgg atcacccacc gttttgcctc
     3901 tgccaaacag tatgttaatc acgcctgccg ggaagatatc tttcgccagc tctgccaact
     3961 tcaacgcggt cagcggggta atttctgatg gtttaagcac tacgcagttc cctgccgcca
     4021 gcgccggagc aagtttccac gcggccatca tcagcggata attccacggt gcgatagaag
     4081 ccacgacccc caacggatcg cgacggatca tcgaagtatg accttcaaga tattcacctg
     4141 ccgccagacc attcagacag cgcgccgcac ccgcgaaaaa gcgaaaaaca tcgacaatcg
     4201 ccgggatttc atcattgaac gcactatgca gcggtttgcc acaattacgg gattccagtt
     4261 cggcaaaaac ctgaccattt tcttcgataa catccgccag tttcagcagg cattccgcac
     4321 gcgctttcgg tgtggtttgc ccccattcag caaatgctgc atctgccgca cgcacagcag
     4381 catcgacctg ctctgcggat acctcggcaa tctccagtaa aacgtccccc gttgccggat
     4441 tatagacagg ctgtttttcc ccttcgccgc taacccgttc tccgttaatc agtaacttat
     4501 gttgcatagc ttattcctgt atcagtggtt attgaccatt gtcgccttcg cgggttagcc
     4561 accaggcccc caggatcggc aaggttgtta ccaacataac cagcagtgcc accacgttgg
     4621 ttaccggtac atcacgcggt cgcccaagct gattgagcaa ccacaacggt aacgttcgtt
     4681 catgacctgc cgtaaaggtc gtaacgatga tttcatcgaa cgacaaggca aacgccagca
     4741 ttcctcctgc cagtaacgcc gaactgagat tcggcaacac tacgtagcgg aaggtttgcc
     4801 agccattggc cccaagatcc attgacgcct caaccagact ccacgaggtg cggcgaaaac
     4861 gggcgatgac attgttaaac accaccacta cgcaaaaagt cgcatgaccg accacgatgg
     4921 tgaaaaatcc cggctccaga ttgatggttt taaaggcggt taatagcgcc agaccagtga
     4981 caatgcccgg cagcgcaatg ggcagcagca ataacagcga aatggcgttt ttgccgaaaa
     5041 agtctcgtct ccacagcgcg gcagctgcca gcgtccctaa caccagcgca attaatgtcg
     5101 ccagcgccgc cactttaagt gacagcgtca cggcatcaag aatatcacta cgctgtgctg
     5161 ccacgctaaa ccagcgcagc gtcaggccct gcggtggaaa actaaacgcc gcatcttcag
     5221 tgttaaacgc ataggcggcg attatcagga tgggaaaatg taggaaaaca acgccgcccc
     5281 aggccgccag tttgaggaaa aacggtgcgc gttcagagtg catcgaacgc tcccagacgt
     5341 ttcacgaacg ccaggtacag tgcgatgaga ataatcggca ccagggtgaa tgccgccgcc
     5401 atcggcatat tgccaatcgc cccctgctgg gaataaacca tattgccgat aaaatatcct
     5461 ggaggaccaa ccagctgcgg gacgataaaa tcgcccagtg tgagtgagaa ggtaaagata
     5521 gagccagcgg caatacccgg gattgccagc ggcagcacca catagcgaaa ggtttgtcgt
     5581 ggacgtgcgc cgagatcagc cgacgcctgc aacaatgacg gcggcaaacg ctcaagcgcc
     5641 gcctgaacgg gcaggat
//
LOCUS       DAJTNP010000174         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTNP010000174 DAJTNP010000000
VERSION     DAJTNP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..411
                     /locus_tag="OO160_003901"
     CDS             <1..411
                     /locus_tag="OO160_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5525818.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO160_003902"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO160_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO160_003903"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO160_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5525819.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJTNP010000175         5580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTNP010000175 DAJTNP010000000
VERSION     DAJTNP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            27..242
                     /locus_tag="OO160_003904"
     CDS             27..242
                     /locus_tag="OO160_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5525820.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            235..621
                     /locus_tag="OO160_003905"
     CDS             235..621
                     /locus_tag="OO160_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5525821.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            629..1315
                     /locus_tag="OO160_003906"
     CDS             629..1315
                     /locus_tag="OO160_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5525822.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="OO160_003907"
     CDS             complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="OO160_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5525823.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1998..3203
                     /gene="tet(B)"
                     /locus_tag="OO160_003908"
     CDS             1998..3203
                     /gene="tet(B)"
                     /locus_tag="OO160_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5525824.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="OO160_003909"
     CDS             complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="OO160_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5525825.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4042..4413
                     /locus_tag="OO160_003910"
     CDS             4042..4413
                     /locus_tag="OO160_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5525826.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4423..>5580)
                     /locus_tag="OO160_003911"
     CDS             complement(4423..>5580)
                     /locus_tag="OO160_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5525827.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1029 c         1215 g         1794 t
ORIGIN      
        1 cactattaag tcatgtagct ggttttattt caattgaacg ttttcaaagt ctagctacag
       61 aaggaaaaat gttatcgcta tcttggtggg aaaacgaata cgcagttctg caatggaaaa
      121 atcatgtttt acatgcgaaa gctcaacaag aagggcgaga gtcaatattt gatttttaca
      181 aaattagtat tgctcatatt actcgcgaat attcatttaa aaaggacaag gataatgttt
      241 gatgttcacg ttgttttaga taatcaaata ggacaattag cattactagg aaaaacatta
      301 ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga tgaatgccat
      361 gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca agctggactg
      421 ttagtacttg cgatccggac accattaatt cgtaagttaa aacaggaaaa accgggggaa
      481 cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt gcaatacagt
      541 gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc atctgttacg
      601 ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag aggttacctt
      661 tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag ttaaaatact
      721 cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg tggcgaatat
      781 atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc taatcacagt
      841 agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg ctgaattgat
      901 ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt ccacgccagt
      961 gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg ccgttaagat
     1021 ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag
     1081 tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat
     1141 ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc
     1201 cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga
     1261 agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt
     1321 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     1381 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     1441 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     1501 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     1561 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     1621 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     1681 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     1741 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     1801 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     1861 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt
     1921 aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag
     1981 tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg
     2041 atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg
     2101 cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg
     2161 ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt
     2221 tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt
     2281 ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg
     2341 catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag
     2401 gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga
     2461 tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg
     2521 ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg
     2581 ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga
     2641 ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta
     2701 ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc
     2761 ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa
     2821 aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta
     2881 tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt
     2941 tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac
     3001 agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg
     3061 ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt
     3121 tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga
     3181 gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat
     3241 aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa
     3301 taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat
     3361 aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat
     3421 aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc
     3481 ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt
     3541 ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt
     3601 attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt
     3661 tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca
     3721 ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa
     3781 cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat
     3841 tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt
     3901 attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg
     3961 tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc
     4021 tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg
     4081 gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat
     4141 gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta
     4201 acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag
     4261 ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgattcccaa
     4321 caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta ttatcggcgt
     4381 aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt tccccaaagc
     4441 gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct
     4501 tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac
     4561 gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg
     4621 aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa
     4681 acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa
     4741 gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg
     4801 tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga
     4861 gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc
     4921 tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct
     4981 cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt
     5041 ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta
     5101 ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat
     5161 gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc
     5221 tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc
     5281 gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca
     5341 gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta
     5401 tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat
     5461 gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt
     5521 tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa
//
LOCUS       DAJTNP010000176         5560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTNP010000176 DAJTNP010000000
VERSION     DAJTNP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..258)
                     /locus_tag="OO160_003912"
     CDS             complement(<1..258)
                     /locus_tag="OO160_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525828.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTF"
     gene            199..384
                     /locus_tag="OO160_003913"
     CDS             199..384
                     /locus_tag="OO160_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525829.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(426..581)
                     /gene="hokD"
                     /locus_tag="OO160_003914"
     CDS             complement(426..581)
                     /gene="hokD"
                     /locus_tag="OO160_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5525830.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1233..1649)
                     /locus_tag="OO160_003915"
     CDS             complement(1233..1649)
                     /locus_tag="OO160_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525831.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1676..1816
                     /locus_tag="OO160_003916"
                     /pseudo
     CDS             1676..1816
                     /locus_tag="OO160_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1816..2841
                     /locus_tag="OO160_003917"
     CDS             1816..2841
                     /locus_tag="OO160_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5525832.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2894..2983)
                     /locus_tag="OO160_003918"
     tRNA            complement(2894..2983)
                     /locus_tag="OO160_003918"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2947..2949),aa:Ser,seq:cga)
     gene            3077..3874
                     /gene="mtfA"
                     /locus_tag="OO160_003919"
     CDS             3077..3874
                     /gene="mtfA"
                     /locus_tag="OO160_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5525833.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3975..4050
                     /locus_tag="OO160_003920"
     tRNA            3975..4050
                     /locus_tag="OO160_003920"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4008..4010,aa:Asn,seq:gtt)
     gene            4212..5474
                     /locus_tag="OO160_003921"
                     /pseudo
     CDS             4212..5474
                     /locus_tag="OO160_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1500 a         1316 c         1288 g         1456 t
ORIGIN      
        1 gaatgtgcag gacggacgcg gtggccgttc ggaataaggg agcctgacga agattatcca
       61 gtgacgataa tcgagactga gggctttctt aatctcgtat ccgtgtctgc ggtagcgctg
      121 aatcagccat tcagcctgtt cttcggtgca ggggtcgtgc tgatacccgt cagatttgaa
      181 tgcatgagaa cgccgcccgt gcctgctggc aggggcggca gagttatccg aattgtaaaa
      241 tttggtatcg tgcgccatct gttttctctg ctggcgcagc aggtgccagt tgttcaggct
      301 gacggatgga ttgtaaacca gaacgaccag aaaaaacaaa acccgccgaa gcgggttaag
      361 tgcgggtgcg ttgaggatgc ctgacacatc agcggtggcg agggatttct cccccgccgg
      421 gtctcttact cctcaggttc gtaagctgtg aagacagcga cctccgtctg gccggttcgg
      481 attcgtacct cgcagaggtc tttcctcgtt accagtgccg tcactatgac ggttaaacag
      541 atgacgataa gggcgattaa catcgccttt tgctgcttca tagcctgctt ctccttgcct
      601 ttcggcacgt aagaggctaa cctacatgtg ttcagcatgg attgagcctc agattaatgt
      661 taagcgtctt gcaggacgcg taatgttaac tggggctttt ctctatctgc cttttggtgt
      721 tcatgcctga gacagatagc ctcaagcacc cgcagtcatt ctacttaact aagatttccc
      781 tgcaaaccgt ttttgtccgg cacagtaaat atccaactaa accaatagcg ttcgctgtat
      841 ttaccgccag tattcaatgc acatgaccgc catgaacacc cctaaaaaaa gggcatttat
      901 atgtccaaac attaatatca aaacatcaat tttttccata taccttgctg tgaagatgat
      961 gggcatacat gatgcgaaca accagaacgc aacaaacaaa aactgcaatg cgtttttcat
     1021 tattccccct acaatcaatg tgcaataaca tttaaacaca cctcaatttg gccggacata
     1081 taaatatcta aaccagaaaa aatcacttac atagcgttac aaactcttta gtctaaatac
     1141 tcatcgtaaa acattcccca tacttatcag tccgttccgc gccaggtagc ttattgcctt
     1201 atctggcaac ctataatcag gtttccgctt tttcagttgg ctggtcgttt aaccgacata
     1261 gttaacccat taatctggtt gccggatgct ggtggatttt cgcgttttaa ttgttcataa
     1321 aagtgcacag ctttaaccag ttcttctgat gtaaccggga ctggtggggc agtgaataag
     1381 gcctgaattt catagttcgg cctgtcgtta caatcctctt ttttcggtac atatttccag
     1441 tcaccaaccc acaactcccc ctgagagtcc ataacacctt ttttcacgta gcgatatcgc
     1501 cacgctatcg gctctgcttc cagcgatgcc cgtgcaattt tgaataactc gccctctact
     1561 cgcgccatcc ctgaattggg gtggcatttc gtaatcgcta tttttaattt ggcttcttcg
     1621 attaattgtt cttttgttaa ttcagtcatt ttcattaccg ccctggtaat gctgccacca
     1681 gcgaaaatgg ttaatggaaa acgggcttcc gttctatacc aatcgtagtg gcaaaccgat
     1741 tgtcagccgg gatctattta cctgcaataa aactttacca ccacgcgagg tagagccgaa
     1801 ttttggtgcg atctgatggg aagacgaagg aaaaatcctg aacacgaaaa attacctcca
     1861 aatgtatacc caaataaata tagttatgta tggaaaccaa catccagaga atctgtaaca
     1921 ctaaccgcca tcaaggatgg tttagctgct ttatggaaaa agtatgagga aactgtaaat
     1981 aatcgcgatc gtgcaatgac attcggtcgc ttgtgggaaa aattcctcgc cagcgcctat
     2041 tacagtgacc tcagtccaag aacacaaaaa gattatctgc aacatcaaaa aaagttgctt
     2101 gccgtattcg gtaaggtgcc ggcagattcc ataaaaccag aacacatccg tcgatacatg
     2161 gacaagagag gggaacagag taaaacgcaa gccaaccatg aaaaaagcag tatgtcccgc
     2221 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
     2281 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
     2341 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
     2401 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
     2461 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     2521 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     2581 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     2641 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     2701 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     2761 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     2821 ccaagaaaat attccaagta attccaagtg tgatttttgt cactgactta atgatgtata
     2881 agtgattgaa ttttggcgga gagagtggga tttgaacccc cggtggagtt gcccccactc
     2941 cggttttcga gaccggtccg ttcagccgct ccggcatctc tccgttcaga tggttgccat
     3001 gatgccagga aatttggcat tttaacagtc cctgtccgtg caattttgtt caagtgacga
     3061 gtttgcgagc aaaacgatga ttaagtggcc ctggaaagta caagaatcag cacatcaaac
     3121 tgcccttccc tggcaggaag cactatcgat ccccctttta acgtgtctga cagaacagga
     3181 acaaagcaaa ttggtcgctc ttgccgaacg ttttttacag caaaaacggc ttgttccttt
     3241 acagggcttt gagctgaatt cattaagaag ctgccggata gcacttctat tttgcctgcc
     3301 cgttctggag ttaggactgg aatggctgga tggttttcat gaagtcttaa tttatcctgc
     3361 gccatttgtg gtcgatgatg aatgggaaga cgatatcggt ctggtgcata accaacgtat
     3421 tgttcagtca ggtcagagct ggcagcaagg gcctatcgtt ttgaactggt tggatataca
     3481 agattctttt gatgcttctg gttttaacct gattattcat gaagtcgctc ataagctgga
     3541 cacccgtaac ggcgatcgcg ccagcggagt tccctttatt tcgttgcgtg aggttgctgg
     3601 ctgggaacac gatcttcatg ctgcaatgaa caacattcag gaagaaatcg aattagttgg
     3661 tgagaatgcg gcgagcattg atgcttatgc tgccagtgat cctgctgaat gttttgccgt
     3721 actttctgaa tatttcttta gcgccccaga actttttgct cctcgtttcc cttcattgtg
     3781 gcaacgtttc tgtcaatttt atcaacaaga tcctttgcag agactgcatc acgctaatga
     3841 tacagactcg ttttcagcga cgaatgttca ttaattaaca actttgaaga ttaattaacc
     3901 aattgaaatg gcttatgaaa tttagtgttg acagacaagg taccgctaag taatatgcgc
     3961 cccgttctca cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     4021 tcactggttc gagtccagtc agaggagcca attttctgtt ttcatgcatc cttgcgaatc
     4081 tttatctgat gttgattcaa caggttagtg tgaaaactct tctcggtgca ttttgatttt
     4141 accctacgca tcgggaaaaa ttggtggtca aatctgggat caggttagtt cgataatgga
     4201 gtgaccccca tatatccctt accgacgcaa aaatccgcac cctcaagcct tctgataaac
     4261 cctttaaagt ctccgattct cacggtctgt atctgctggt caagccgggt ggctcccgcc
     4321 actggtatct caaataccgt attagcggta aagaatcccg cattgcgctg ggtgcctatc
     4381 cagccatctc cctgtctgat gcgcgacagc aacgtgaagg tatccgtaaa atgctggcgc
     4441 tgaatatcaa cccggtacag cagcgggctg ctgaacgtgg ctcacgaaca ccggagaaag
     4501 tttttaaaaa catggcgctg gcgtgacata aaagtaacag gaaatggtcg cagaacaccg
     4561 ccgaccgtct gcttgccagc ctgaacaatc acatctttcc ggtcatcggg aacctacctg
     4621 tatcagaact taaaccccgt catttcattg acctgctgaa agggatcgag gaaaaaggtc
     4681 tgctggaggt tgcgtcccgc acacggcagc acctgagtaa cataatgcgc catgcggtcc
     4741 atcaggagtt aatcgatacg aaccctgcag caaaccttgg cggcgtgacc acacctcctg
     4801 tcagacggca ctatcctgcc ctgccgctgg agcggctgcc tgaactgctt gaacgtattg
     4861 gggcatatca tcagggccgt gaactgaccc ggcatgccgt tctgctgatg ctgcatgtgt
     4921 tcattcgctc cagtgaactg cgtttcgccc gctggtcaga gattgatttc acaaaccgag
     4981 tctggacgat acccgcgacg cgagaaccca ttattggcgt gcgttattcc ggccgcgggg
     5041 caaaaatgcg aatgccgcat atcgtccccc tctcagaaca gtccatcgcc attctgaaac
     5101 agattaagga tatcaccggt aataatgaac tgatcttccc cggcgaccat aacccgtata
     5161 agccaatgtg tgaaaacacg gtcaataagg cactgcgggt gatgggttac gacacgaaaa
     5221 aggatatctg cggtcacggc ttccgggcaa tggcatgcag tgcgctgatg gaatcgggtt
     5281 tatgggcaaa ggacgcagta gaacgccaga tgagtcatca ggagcgcaat accgtgcgca
     5341 tggcttatat tcataaggca gagcacctgg aagcccgcaa agcgatgatg cagtggtggt
     5401 cggattatct ggaagcatgc cgagaatctt atgcaccgcc ttatacaatt ggtaaaaata
     5461 agtttatccc atagtatact aacgaatccc acactctggc aaattaccac cacctctaca
     5521 ggaggtggtt ctatatagac ttttatcaac ttcatgacga
//
LOCUS       DAJTNP010000177         5434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTNP010000177 DAJTNP010000000
VERSION     DAJTNP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            40..234
                     /locus_tag="OO160_003922"
     CDS             40..234
                     /locus_tag="OO160_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5525834.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            501..1427
                     /gene="tauA"
                     /locus_tag="OO160_003923"
     CDS             501..1427
                     /gene="tauA"
                     /locus_tag="OO160_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5525835.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1440..2207
                     /gene="tauB"
                     /locus_tag="OO160_003924"
     CDS             1440..2207
                     /gene="tauB"
                     /locus_tag="OO160_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5525836.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2204..3031
                     /gene="tauC"
                     /locus_tag="OO160_003925"
     CDS             2204..3031
                     /gene="tauC"
                     /locus_tag="OO160_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5525837.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3028..3879
                     /gene="tauD"
                     /locus_tag="OO160_003926"
     CDS             3028..3879
                     /gene="tauD"
                     /locus_tag="OO160_003926"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5525838.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3985..4959)
                     /gene="hemB"
                     /locus_tag="OO160_003927"
     CDS             complement(3985..4959)
                     /gene="hemB"
                     /locus_tag="OO160_003927"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5525839.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1262 a         1345 c         1512 g         1315 t
ORIGIN      
        1 tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag gaaaggtgtt
       61 gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact agagggccgt
      121 aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag agagattttg
      181 gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg ttaacttcat
      241 tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga catttttgaa
      301 gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat cgatatcgaa
      361 gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac ttattgaatc
      421 ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt acctagtcat
      481 ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg gtgaacgtca
      541 ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac aacacctttg
      601 ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc agcatcgtgc
      661 gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca ttagcggttg
      721 cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg ggtaactccg
      781 aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt aaacgcattg
      841 ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa cactggggga
      901 ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc gccgcatggc
      961 agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc ctggaaaaag
     1021 acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca acgctggacg
     1081 tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa gcgttcgcca
     1141 aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg ctgaaacagc
     1201 cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac gttccggggc
     1261 tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg accggaccgg
     1321 tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag gtcccggctg
     1381 tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag gaggcgcaga
     1441 tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg gaagatatca
     1501 acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc tgcggtaaaa
     1561 ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc attcaactgg
     1621 cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag aatgaagggc
     1681 tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg gcaggtatag
     1741 agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg ctggaaggcg
     1801 cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg gggattgccc
     1861 gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt gcgctggacg
     1921 ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag acgggcaagc
     1981 aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact gaactggttc
     2041 tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt gctcgccgct
     2101 ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc gccatgcgcg
     2161 aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg tgctcattaa
     2221 tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc aggtgacctt
     2281 aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg cgctgcaact
     2341 gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc tcaccattgc
     2401 cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca gcctgacgcg
     2461 cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga tcgccatggg
     2521 gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc gtccggtgcc
     2581 accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa cctcgaagat
     2641 cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg caggggtgaa
     2701 aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc gtgcgcaggt
     2761 gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat tacgcattgg
     2821 tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga cacgcggttt
     2881 aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac tggcggggat
     2941 cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt tacagcgccg
     3001 tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac cccgctgggg
     3061 ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag cgataatcag
     3121 tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg cgatcaagct
     3181 attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca tattcaccct
     3241 gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca taacgataat
     3301 ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc acccgcaggg
     3361 gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg gaccagcggt
     3421 attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg gctgcgtgcg
     3481 gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga ggaggaacat
     3541 caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt ggtgcgaacg
     3601 catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac gcgaattgtc
     3661 gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca tatcactaaa
     3721 ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg ggataaccgc
     3781 gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat gcatcgggcg
     3841 acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg acggttccct
     3901 cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca acaggttaac
     3961 tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca aatccagcgc
     4021 aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc tttcgagcac
     4081 gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg cgtactcacc
     4141 gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac gcacgatgtc
     4201 gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt catccagcag
     4261 tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt tgcggtcgcc
     4321 ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg cgaacttggt
     4381 cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct gacgaatcgc
     4441 ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg cagcagctgc
     4501 aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc catgctcgca
     4561 cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg acatgacgat
     4621 catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc catcttcccg
     4681 ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca ttacggaacg
     4741 gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg gaatgcgcat
     4801 cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa caaagatcgg
     4861 caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca gcgcaggaga
     4921 tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga tgtttgtgga
     4981 ataaggtgca cagtatacct gaagcggggc aggagtgttt tacgaaagtt gtcgcgatgt
     5041 tgacaagaag agaatgaaag agagaccagg acatctttgt aatagaaata gcaataattt
     5101 catattcact gaaaatattt ttaatcttta tttatagctc ttggttatta ttttttggcg
     5161 tttggttgtg ctgctataaa ttgaataatt caaactttgt tgtatattga taagatattt
     5221 atctggtttg tatttttgat atttataacg cgtatgcatc aaaaataaca ttatctgcaa
     5281 catactgaaa tatcgtcctt ttatcaattt gatgcgtgtt tttctccata aattatatat
     5341 gtccacattc ggacttaggg gaaagaataa ttgaaccatt cgtctgtaac gcagcataat
     5401 cgttagcgcg aaacataata tgttttctat gcat
//
LOCUS       DAJTNP010000178         5379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTNP010000178 DAJTNP010000000
VERSION     DAJTNP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..487
                     /locus_tag="OO160_003928"
     CDS             <1..487
                     /locus_tag="OO160_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5525840.1"
                     /translation="FPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIAN
                     EPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAA
                     PSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESA
                     H"
     gene            509..2500
                     /gene="cyoB"
                     /locus_tag="OO160_003929"
                     /pseudo
     CDS             509..2500
                     /gene="cyoB"
                     /locus_tag="OO160_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2490..3104
                     /locus_tag="OO160_003930"
     CDS             2490..3104
                     /locus_tag="OO160_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5525841.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3104..3433
                     /locus_tag="OO160_003931"
     CDS             3104..3433
                     /locus_tag="OO160_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5525842.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3445..4335
                     /gene="cyoE"
                     /locus_tag="OO160_003932"
     CDS             3445..4335
                     /gene="cyoE"
                     /locus_tag="OO160_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5525843.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4519..4830
                     /locus_tag="OO160_003933"
     CDS             4519..4830
                     /locus_tag="OO160_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5525844.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4814..4972
                     /locus_tag="OO160_003934"
     CDS             4814..4972
                     /locus_tag="OO160_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5525845.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4965..5363
                     /locus_tag="OO160_003935"
     CDS             4965..5363
                     /locus_tag="OO160_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5525846.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1171 a         1339 c         1419 g         1450 t
ORIGIN      
        1 tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt
       61 cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca
      121 tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg
      181 cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg
      241 ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact
      301 ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt
      361 tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga
      421 aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc
      481 ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc
      541 gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct
      601 cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc
      661 cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg
      721 tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggtgaagc
      781 gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat
      841 cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat
      901 cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt
      961 tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct
     1021 ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg attactggat
     1081 atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac
     1141 cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat ttacctaggc
     1201 atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc
     1261 gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa
     1321 catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat
     1381 cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt
     1441 tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt tcatcgtttg
     1501 gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac
     1561 cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta
     1621 tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta tcgtcacctt
     1681 ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca
     1741 taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg
     1801 ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaacgaaac
     1861 ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct
     1921 gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt
     1981 ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg gtattctctg
     2041 cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg
     2101 cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa
     2161 ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga aagagaaagg
     2221 cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc
     2281 aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg
     2341 gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga
     2401 cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt
     2461 cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg
     2521 cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga
     2581 tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg
     2641 tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg
     2701 ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt
     2761 acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg
     2821 gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc atggggccgg
     2881 atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca
     2941 cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc ggcctgacca
     3001 gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt
     3061 ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga
     3121 tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat
     3181 cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc cggccgtaat
     3241 tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct
     3301 gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct
     3361 aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca actacaacat
     3421 gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca
     3481 ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc
     3541 agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg
     3601 ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag
     3661 aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg
     3721 ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg
     3781 ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa acgccactca
     3841 gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat tggctactgt
     3901 gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg
     3961 cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca ggcggcaaac
     4021 attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat
     4081 atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa
     4141 tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct gcgcggttat
     4201 aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat catcgccatc
     4261 actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca tacgctgctg
     4321 gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct ttttattgtc
     4381 tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga tgcacataaa
     4441 ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag
     4501 ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt cgataaaacg
     4561 ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa
     4621 gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc
     4681 gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg
     4741 atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa
     4801 aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg
     4861 taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt
     4921 tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt aatagcgact
     4981 gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc tgtactttta
     5041 cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca caaccttcag
     5101 aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa aagcggggct
     5161 ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac ccggcattac
     5221 caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat gctcgtctcg
     5281 gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg tttttggcga
     5341 ttcagcatat tctcgcggct tagattatta tcgccaccc
//
LOCUS       DAJTNP010000179         5299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTNP010000179 DAJTNP010000000
VERSION     DAJTNP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..739)
                     /locus_tag="OO160_003936"
     CDS             complement(<1..739)
                     /locus_tag="OO160_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5525847.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVK"
     gene            complement(751..1240)
                     /locus_tag="OO160_003937"
                     /pseudo
     CDS             complement(751..1240)
                     /locus_tag="OO160_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1244..2464)
                     /locus_tag="OO160_003938"
     CDS             complement(1244..2464)
                     /locus_tag="OO160_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5525848.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2468..3211)
                     /locus_tag="OO160_003939"
     CDS             complement(2468..3211)
                     /locus_tag="OO160_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5525849.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3102..4568)
                     /locus_tag="OO160_003940"
     CDS             complement(3102..4568)
                     /locus_tag="OO160_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5525850.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4568..4801)
                     /locus_tag="OO160_003941"
     CDS             complement(4568..4801)
                     /locus_tag="OO160_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5525851.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(4789..5268)
                     /locus_tag="OO160_003942"
     CDS             complement(4789..5268)
                     /locus_tag="OO160_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525852.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1132 a         1493 c         1325 g         1349 t
ORIGIN      
        1 atttaaccgc gcggatattc agcggaacac cgttctggtg atagctgatg gtgttggtct
       61 gaaggttcgt caacagggac tggttacctg cagatgaaac gatgatgctg gacaacagag
      121 caaactgctc aggcgggatc agcaaatcac gcggaaccac agagtaacca gaagcggccc
      181 acgcatcaga cagcacctgg ttaatgcttg cgcggatttc gtccggtgtt gaggttgccc
      241 acgttttggc agcgttgttg acaggcacgc cgtccagggt aacaaggcct ttcaggttta
      301 atgcggaatc gccaacatat acctgttcat cgttatccat ctgccatttc agttgcatac
      361 cgtcatactt ctgcgtatca atcgggcggc cgacctgctg agcagcctgc aattctatga
      421 ccgtccagcc aagttccatc ccccacaggt tcagcgggtt accggatttg ccgatatcca
      481 cgttcacgcc agcaatagcg gttgagtctt tgcctaccca gttttttcca ttcggatttg
      541 cgccagtacc cgcagcggcg aagctggtat tcgtccagct ggaaatgtca tctgcgatgg
      601 aggcgtcttc acgcagttga atatcgcggc tccaggtgta ccccaccagt ggcaggttca
      661 gcgtctggtc gagtctctcc agctccccga tgagaaaggc accagagctg tcaacggttg
      721 cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc caggaaattt ctgcattgcc
      781 gtcagcatca ccggcacctg tgaattcagc gttggtcagc accacgtttt tgccactgac
      841 tgacgtggac atgaatccac ccagaggcac tttgatggat tcatcagtgg agatcgcctt
      901 ttttgatagt gctggcatca aaatcagaac cgagattaac ggtcacgtag ccacgcttca
      961 tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg aaccatgtcc ggctgcgacg
     1021 tggtcggata agggcgcacg tagatcccct tcaccttgtc tgcggtatca ccatctgcca
     1081 gcggcacgaa aaaaccgtca tcatcgtatt taccagccag gccataggca gcgaaggcgt
     1141 tatcggattt aaggaccacc ggttcgacgg ttaagtcctg cgggcgagag acagccccgg
     1201 caatgccaac aggcatccgg tacagaaata cattattcat tttttaccct ttacggtttg
     1261 cccagaattc agcgttttgt ttgttcaggg aagcgatact ggtcatgccc atgtttaggc
     1321 gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt ggcaacctca gacacggcat
     1381 taaacgccat gtcgaccgat tgtttcggta atttgcggat atccgcatca ccgactatct
     1441 ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg tttgaacgcg gtcggtttca
     1501 tcttacggct cagatcgata cccgggacga taacttcggc acgccaggct gagtcaccag
     1561 taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt cggattatcg tcaggcttat
     1621 tatcgttatc gcccgtggca tttctttcca gcttagccag cagggctttc agtaatgttt
     1681 tgaggtcatc atcactgtcg ccggttggat ctccgcccat ctctggtgct ttgtccggta
     1741 gtggttgctg cggggacagg ttgatgttga gattaacgcc ctgcggcaaa tccccctcat
     1801 ctcctgtaac cgatgcggga gccgactcca ccagttcgtt catggtgtcg gcatctcctg
     1861 tcttgatggc cgcacgcatg cggttccacc agtttttctt ttgatttgcc attgtgtctc
     1921 tgtctccaat tgcacaacga tttccggctc tgcctttagg gacaagagcc acatggtttc
     1981 cggtaatatc gacctgctcg gctttacctg gctcggtctg ctcgtactcc gcgtcatagc
     2041 cgcacgacac ttcgcgcagg ccatcttcga taagctgaat ggcgctttcg tctttgacga
     2101 taaggtcagc cagcatcaaa tcagactgct cacccgtccc gcgccggaca ttctggaggt
     2161 gcccgacagc aagcactttc cagttctcgg gatttaccag ccgcacattc ccgttttcat
     2221 cttcaggatg caggatcgtg atgctcatcc cttcgaatga ggcaagcgtg gccggatgga
     2281 atacctgctc aggagaacgc gtgacgacta tttcaacgaa cttatcgggt ttcagttttg
     2341 gcaggtcatc agcaccatag agctgcttac ctgttcgtcc tatcggcacg tctttgcaca
     2401 gcaacgagcc gtcagccagg cggtatcggg tttccccgag cctggtctca aaaaagtatt
     2461 tcatgggtta tgctgccctt ttcattgcga atggaacgcg aacaaacacg atctctttat
     2521 agcagcggca gtttggcagc tcgcccgcgt ggccggtcat gccgtccaaa gttggaggtg
     2581 aatcccagcg gacgaattta cctttcatgt gatcgtggga atctcggaca tcgccatcat
     2641 cggctgtacg ccaaatataa ccatccgagc caatagctat cgctcgggcc atatccagcg
     2701 cgcccgttgc tcttcccagt tccgttcggg caatcagatc ggccctagac ttagcaacat
     2761 cgccggtaga tgcaatctct ttagcaaact gctccgcccg accaccggtg acaacagcct
     2821 ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc ctcgagaggc agagatttga
     2881 tgtacttgac ctgttcggcg atgatggatt gcatcacctg gcccacagga gcgctttcca
     2941 caagattgcg gagctcgcga ctgatgttct tgctgtgttg ccgccaaact ttctcgttct
     3001 gccgggttag gtccgcagta aagttttccg cgacctttgt cgcccagggg gtgatgattt
     3061 cactgtaacg ctccagcgcc gcaataattt ccgtgatact gtcatttgaa ccatcgtagc
     3121 gaccatttac gatgtctccg accgccctcg ctatcctgcg taggctggtt cgataccgga
     3181 tttccgcctg acggctcctg cggttcgtca tcaggttcgc cgatgccggg cggcgcttca
     3241 tcttcggcat tctcgatgtc ctcgttggta atggatgccc ctatgccggt tacgtcagaa
     3301 ttttctcgca aatcagtcat agcggctttc agtgtcatca gaccatcacc cagcgccgta
     3361 ctgattgcgt tggtggtgtt taacgccacc gttgagcgat cgacatcaga catttgccag
     3421 agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct tgccaagttc cgaacgatgc
     3481 atgatgtcca gtatccgccg caccggaaga cgtaaacgcc tctcctgcaa cgaactgatg
     3541 cgatcgtaat agttggcaag gtctgcatca ccggtagaaa atcctttcgg ggactgtccg
     3601 aacaaccgca ccagtgggat accaacagcg ccactaatct gttctgcaaa ctgcgaaagg
     3661 atgtcatcca gaccactgaa gctgtactga tgggtttcaa acttatcccg cgagtccatg
     3721 agcgtcatgc cttcattact ctggaactgt cgaatcaggt cgatattctt cagcaacgct
     3781 tcatacgcag gaccaccaag tgcgataagc tcgcgtagct tctccacgct gtaggtacgc
     3841 aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag cgctgtcgaa cgcggtaaga
     3901 cgatcccaga tacgctctac aaccgacatt ccccattcgt tctcggtcat cttctgctga
     3961 aatggcaacg tgacgccatc aaagcgaatc aggcgactgt gatgaatgcg ccaggcagga
     4021 attcccgttg ctgtggtcac cacatcgtaa aactcaggtt tacccaggtc cggccccata
     4081 tctttaatgc ggcgggtcag tgccgggtcg accatccagc ggtcgagcgg gagaatcccc
     4141 ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg tcattggtgc ctgcccctca
     4201 atcatgatga aacccaccgc gccgccgtag aggcgcgacc atttcagcac gtcattcagc
     4261 gcatcccaga tttgcaactc atccagttgt gattcgagaa tgccacgatc ttttgcatca
     4321 atttccgaag tgatgcgaat gcctttgcgg gtcatatcat ccgggatagc atcgactgct
     4381 tcgccgatga tccaggatga acgataggac cattccacca gcatgcggtt acgactggtg
     4441 aaattagccc ggtaggtgga tgctgagtgc tggttaggtg tctgcatccc tactcgggca
     4501 ataaaattct cataaccatc agctgtggcc tgcacagttc gccgcagggc ttgtttgttt
     4561 cgtgccatca ggcctgtctc cccagctgtt cccagatatc cagcgatgta tcgattggcg
     4621 cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg tatctcgcgt tttgcgaggt
     4681 cttttttact ttcgaccatc acacgtccgt tacggtcgaa atcacgatga ggtgttgtca
     4741 gttccagttt cagcttttca agcaacggac aacgagaatc tatgctgatc agctcatcca
     4801 caggatactg ttctccgttg ttaatggcgt taaacgtatt tctgaaacgg tcagccacca
     4861 tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg atgccgttgt actcgtcatc
     4921 tggctcatgc acaccagcac cggcgttaaa cctctggtaa ttcacacgtc gcgcgtatgc
     4981 gttctcgctc ttccggtcag cgttaatttc agagaattta gcaccggcag acgcaccaac
     5041 accgatagag tcgtaaacaa tatctgcttc acgctccagc gccgcctgat aagtacgctg
     5101 gcagctcttc agtaattcat cttctttggc cttccattcg tcggcccaga aaacaacgga
     5161 tccgtgacgg taaacgttag cgcacttatc tgtaccactg tcagccacgt caaagccaat
     5221 acgctttctt ccactgggtt cgaaatttaa cgttttgtgc gcatccactg cggcttctat
     5281 ccaagacagt ttgatgatt
//
LOCUS       DAJTNP010000180         5218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTNP010000180 DAJTNP010000000
VERSION     DAJTNP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO160_003943"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO160_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5525853.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO160_003944"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO160_003944"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5525854.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO160_003945"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO160_003945"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5525855.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..>5218
                     /gene="clpB"
                     /locus_tag="OO160_003946"
     CDS             2743..>5218
                     /gene="clpB"
                     /locus_tag="OO160_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5525856.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQI"
BASE COUNT         1277 a         1265 c         1424 g         1252 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgaccttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcg
//
LOCUS       DAJTNP010000181         5190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTNP010000181 DAJTNP010000000
VERSION     DAJTNP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            486..1277
                     /locus_tag="OO160_003947"
     CDS             486..1277
                     /locus_tag="OO160_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525857.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1663..1869
                     /locus_tag="OO160_003948"
     CDS             1663..1869
                     /locus_tag="OO160_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5525858.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1955..2527
                     /locus_tag="OO160_003949"
     CDS             1955..2527
                     /locus_tag="OO160_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525859.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2529..2813)
                     /locus_tag="OO160_003950"
     CDS             complement(2529..2813)
                     /locus_tag="OO160_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525860.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2784..2927
                     /locus_tag="OO160_003951"
                     /pseudo
     CDS             2784..2927
                     /locus_tag="OO160_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4096..5115
                     /locus_tag="OO160_003952"
     CDS             4096..5115
                     /locus_tag="OO160_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5525861.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         1667 a         1147 c          981 g         1395 t
ORIGIN      
        1 ggactacatt acagcagaga ggggtaagga cagagttaca agtactacta gagagaatat
       61 cactgcacag atacttctat gagtatatct ttatgagaga tccgtctaca ggattgggtt
      121 ataaggtgta tttacaaggt ataatcacag gatcatttac atgaccatac ttctgtaaat
      181 aatactaatt aaccttaatt atttttttat ggtacatgaa ctacctgtag ctgtaacgat
      241 aaacaaatca cccaaaatga aaaatactgg ttatcacagt tcagagaact cgtatgagtg
      301 tccccctgag caatgcttca ttttctgtga ttctgtgtga tagagttata ctgagtacag
      361 tggcagtaac actgctttcc atcacttgag gataaaattc attaaaaaat taattgcatg
      421 aattaacagg tttttttatg aaatgttatg catctcaacc aatatgatta acagaaaact
      481 tcatcatgac aacacgtata acgttcagta tgaccagtga tgagacgctg cgaattgtgg
      541 atgagtactg tcatacccac aaactttcgc gctctaaggt aatcaacgca ttactcagcg
      601 ccacagcacc tgtccttaat gatattaact gttattatca acttgcaggc gaactgcagt
      661 cacgcttatt aaatggagtg tatcaacggg atcttcctcg caaacgcaat gtcgtcagtg
      721 cagagaagta ttgtctggaa atctgggaaa acaaactttt caccagaaga atcctggagt
      781 ttgacagttc aaatggtgtt ctgtacgcgc tgaaacataa aagacactat cgccgggata
      841 agatgattgg aagggttgaa agtcggtgta tcaaagacat ttgcgaatac cagatgcagc
      901 tatccgggga gaagacgaaa tatgcctgtt ttatttatat cgaaagaaca atatacaatc
      961 acgataatcc atctgataaa atacctgtaa aagcggctgt tggtaatgca gtcatcctgc
     1021 tggcaaagga tgtgatatat aacgagtact tttttgattt gcggcagtca ttttttgtga
     1081 gcgttacaga tctgatggtc tctggcgcaa aaggtattcc ggagacacag acatatccgg
     1141 acgtttattg ctggatccct cttttttcca taaattccgg cgtgctcatt acccctgtat
     1201 ataaaattga ccctctgaaa cccgtaacgg taaaaaaacc ggataagatc actgttgttt
     1261 gtaattacag agaataattt cagccttcta ccttcaccgc gacagtgttt acctctgctt
     1321 tatcttccgt tcagtaaata caaaccaccg ctctggcagg tacagggcgt ggtttatctc
     1381 cgatactccc ctgtgcagta ataatcatca ctgaaaatac tgatcccgtc acggcaaaac
     1441 gccatcccat attccggcaa cacaaaatca tcttccgttt ataccggatt cactgacaaa
     1501 acagacaacc tgtattgcct gtacacaaca gtttctctcc tggcaggaat attcccctac
     1561 tcatttttta cgtttctccc cggttgttat gccggtgaag gtggtgcgtc gttttcatca
     1621 ccaacgccgg ttgagattaa caacatccgg aggaacattc tcatgaatac cccagtttca
     1681 ctgatggatg accagatggt cgacatgtcg tttatcactc agctgaccgg cctgaccgat
     1741 aagtggtttt ataaactcat caagcatgga gcctttccgc cccccatcaa actgggccgt
     1801 agctcccgct ggctgaaaag cgaagtggaa gcctggctgc aggcgcgtat tgcacagtcc
     1861 cgtccgtaat tcctgaccgt tatccgttcc accgcagcaa aggcatgatc cggcccccgg
     1921 aggcattttg ctgcgcgcta aaggagagaa tattatgcaa caacttgaaa acaggtgtga
     1981 cctgctgctc atccagcatc agaaatggat gacaagcgtg acgaggctta tcgtcgctca
     2041 tggaatgggg tctccgcatc ttcatggtta tcaccgtctg accctggcac acttttttct
     2101 gccggaaaaa ggctccgtca tttcggtggc cccccaggga ctctatcagg tcgtcaaccc
     2161 gggaacacca ccgttcattc ctgccattca ggaagggtta atgaccagca ttcagaccca
     2221 tgaaatcatg ctgcttacac attttaatct gggcggcgtt ttactcagtg agctacatcg
     2281 tctgggtgaa aaccggctgg caaacaggct gaacagcctg ctccggcgtt tcgacgatcg
     2341 tgatctctac cacacgctga tatggctgtg ctggtatgat ctgatgtgtg cccactccat
     2401 gcagccctgg actgaagagt taaagcataa atcacatgca gagcttgaaa actgggctgt
     2461 ggcgcgcaaa cgggaaaagc gcgagctgga actgatgatt gacgagtacc tgttatacgc
     2521 ctgctgattc ataacgttcc cggggttcct tcctcatcgt tacacacagc cggacggcta
     2581 tctatggctg tccgccaaat gaccatcgcc gcatttcgtt ccgctgaaaa cggtacgtag
     2641 tcagtgatac cggaatttcc ggagagggcc gcaggtatat tgcttctgtg caattgatgc
     2701 ttatttacat tggatgtagt tatttctgca tccaatgcta tcggaaagag attaaccagc
     2761 aaatggttcc tcaaatcgta ttgatattta cccggcgtat tgagcaccac catctgacac
     2821 tccgttcccg catcaaacga ctggaccgaa aaacgattta cttttcacgt gctgtggaaa
     2881 ttcacgaaaa aattattggg tcattcatcg aaaaacatat gttctgatga ctggagtcat
     2941 cctccagaaa tatacgtcaa ctgaacaata tcgatttttt atgggattct atacactttt
     3001 ccatatccct ctgaaaatca gtcatcagct caccaacacc tcttctgaaa cttgtattaa
     3061 acttattgct taaatagatg atataacatc tcttccattg cactgaatcg gcgaaaactg
     3121 ccgtttcaat aatctcaaca atattattta tcggctaact ttatcagtaa acaatggaat
     3181 gtcacactga tgaaatgaac attcagagta agatgataaa attcatataa acgccattgc
     3241 tgaatattat ttatttcata cagataaacg ataaaataca aaagggtata tttctcaatg
     3301 taacagtcaa ttattcatct ccactggcga gcacggtcag cacacaaaca aagttagtcc
     3361 tcctgcttaa tcattgcgta tcagaaacta aaaaagaaaa aattctgcag acaacttaac
     3421 aatcttcatc acatttcctg aagacagaca tatccacaaa cggtagtcag cgttgaatta
     3481 acaagtctta cagagctata cgtagcttat aaagccactc aatgttcatc atcacccgac
     3541 tacttatctg gaataccacc agtataaact catcaataaa aacacctgtt gttaaaataa
     3601 gaatactgtc agcagtaaat tattaaaaca acatctgtcc acactatctg ttcttttaac
     3661 gccaacggca gcgaaaatag tgcatcacag ccttcttttt tctttcgcca ccactcagac
     3721 aaacacaaca ctcacaccca caccacaaaa cagaatcaaa acataaaatt aacaataaca
     3781 gtcaaaagga aaacacagaa tattgaggtg gactgtcacc aggacagcgc atacgacgca
     3841 tgtgatcagc acatcccgtt cttcgctggc atccccaaaa tgcagaacaa acaatcataa
     3901 cattatgaat ataaaagaaa atatgatttt tcaccatgaa aaaaacatca atgtatctct
     3961 gtataaaaat aatgataaca tgacataaac atttaaaaac ataaataaac agcaacaaaa
     4021 acatttgata atcacttagt gattaaatac aattaagacg attttgttat aagagaggca
     4081 ccatggagtg agataatgaa taacgaacag atgcttatca aatgcagaga tccccgtaaa
     4141 tgtcggtttt atgccgaata tctgaatcac aaatacccta atgaattccc tcactttgcg
     4201 cctgacaacc cacaaaattg tatcaggaat gttaatttat ttttaaagga aaagtattgt
     4261 aatgtttcac ctcccccctt tggtactttt ggtactactg aagaagaagc actcaataac
     4321 gaaattaatg agcttgaaat tattgccggg caggcaatac tgcctgaaga gagttttctc
     4381 tggttgaaaa ctgacgaact tgcgactttc tttacctgga ctactattta tctgtcgaag
     4441 gaagaccata caaaattccc gaaaataaac cataattcat caggaataaa aaacggaatt
     4501 gaagtcagga aaatacgaac agttaccgat gaaacgttaa acacttacaa aaaaagtaac
     4561 cttccggata atccatcatc ccataacgaa cgaataaaag taatcatttc atattttgac
     4621 tcctgccatt ctttaattaa taaaaatgca aaaagcatat acctcgagga actcaggcag
     4681 gcgtggctta aaaagaaaag cgcttgtcaa aagtttaagt ggctccttcc tgacgatgat
     4741 gaaatgtgtc agtggttctg gacaagactg aaacaaaaac aggatgaaaa acatccagta
     4801 cataacccag tgacccggtg gttcatacca tcttcaacga aagaatatta tcttgcctct
     4861 cttgtcgcct ttgctttatg gaaagaagcg cctgatacca ttgaactgtt cagaacacga
     4921 ataaatcatg cctggcatca gaaaaaacaa aaggacaaat atcggagtga tggtaaaaag
     4981 gccattaata tacatataag aagcaatgtg aaagaaatgc ttgatgagct cagaactgtt
     5041 tacggtctga gaacaggaga gttttttgaa atgctgatta cagagaaata tcttgaacac
     5101 aagcgaagga agtaattcat tacctgttac attctaaata atctcagaca gatattatct
     5161 ccgtgaatcc cccgccacct ttccggtgcg
//
LOCUS       DAJTNP010000182         5169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTNP010000182 DAJTNP010000000
VERSION     DAJTNP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..338)
                     /locus_tag="OO160_003953"
     CDS             complement(<1..338)
                     /locus_tag="OO160_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525862.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFT"
     gene            complement(406..2355)
                     /locus_tag="OO160_003954"
     CDS             complement(406..2355)
                     /locus_tag="OO160_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5525863.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2440..2775)
                     /locus_tag="OO160_003955"
     CDS             complement(2440..2775)
                     /locus_tag="OO160_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5525864.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2775..3131)
                     /locus_tag="OO160_003956"
     CDS             complement(2775..3131)
                     /locus_tag="OO160_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5525865.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3131..4627)
                     /locus_tag="OO160_003957"
     CDS             complement(3131..4627)
                     /locus_tag="OO160_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5525866.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4624..4785)
                     /locus_tag="OO160_003958"
     CDS             complement(4624..4785)
                     /locus_tag="OO160_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5525867.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4795..5136)
                     /locus_tag="OO160_003959"
                     /pseudo
     CDS             complement(4795..5136)
                     /locus_tag="OO160_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1217 a         1518 c         1130 g         1304 t
ORIGIN      
        1 gtaaaaccaa cagtatctga atcaaaatca tcatctttat atattgggat caaaaatgtc
       61 atcgtcgctg gtgatgcatt gtttgcaaaa tcaaatacac ctatactttt gccgaattca
      121 taagaagctt ttctcaatat aatcgcaccg ctggcagaag gggatgggca cgatatcctc
      181 agttgtctga atccatctgt atccaagtca acgtcattat ctgcagatgt tttaacgtta
      241 ctaataaatc gtgaaacaat ctcctcattt gttagtattt tcgcctgagc acagcaagag
      301 gttaatattg caaccgtcat taaaaatggc tttatcacaa aacaaactcc attgttagca
      361 tgttacgcga taattttgcc tcatctttca ccacaaaaaa agaactcacc ttttaaatgg
      421 agaataccca acatcatggg tgattttcat aaagggatcg cctgttttcg gcaaatcgat
      481 aacgcgcatt ccaggcggcg cgttgtcaaa cgtcacctta agttcgctgc gggttgccgg
      541 tgaagatgca aggcttaaca gctgattcct ttccagtgat acattcgggg tgtagcgttt
      601 atcgggagat aaatacccat caccacgtgg catctgccat ccggtcatgt catagacaaa
      661 atcatggaac tgtttgcccc actcgtcgaa cttatcattc agaccaaatc cactgttcag
      721 tgcatcagcc acaaaatttt taacaagcca gggatgttct ttttcaaact cctgcgccat
      781 catgcccagt tcaagcaatc caccaatcag actgatcttt ccgaggccct ttaatttcag
      841 ccctgttttt cctgccgccg ttgtccattg attctgggcg actgttgccg cacgaatact
      901 tgccaccgcc ttcacaccaa tatacagggc agaaaccgtt gctattgttt ttactgcctg
      961 ttcccatccc cccattgctc tgacaacctg atcaacctcc tgccagactt ttttcacaac
     1021 cggaccgacc gtttcccagt tatcaattat gagatatgca ccaccgacga gaagcgcaat
     1081 taatcccttt gccggcgtca tattcatcac gctacccatg atttttgtta tgcgtgtcaa
     1141 tgtgccaatc gccacgccca tcgtcagtaa tgccgcacca gttttcgcga tggtttttac
     1201 gacctcagga ttttctctga caaacgtgcg cacttcttcc agaaaaggct tcatctcttt
     1261 tatgccttca ttgagtgaag gcaggaaggt ttcccccagc gtggaagaaa tcgcattgat
     1321 ctggttctgc agcagcaaca actgattttc tgtcgtcgcg gcacgggcgg catattcttt
     1381 ctgcattgag ccgccatact gctgcgcatc cgccacacga ttaaagttgg ttcgcaacaa
     1441 atccagattc gtgagcagcg gcgcaatggc ccccagggat tccttcccga acagcgcttt
     1501 cagcacagca gcctgttttt ctttcggcac ttttgccaga gaatccagta cgtgcagcat
     1561 ggccccgcgg gcgtctttct gcatatccgc cgccagtttt ttcgggctaa tgcgcaacga
     1621 gcggagcact tttttctgcg atttggtggc agaatccctc gctgtcagcg acaacataaa
     1681 attctttatc cccgtcgcag caatttctga ctccaccccc attccggcaa tggttgcccc
     1741 cattgcggca atctctccgg aggccacacc cgcaacactg cctaaagggc caatacgggt
     1801 cacaacatca gaaatctttt tcgcacttgc aggaccggta ttgccaagat agttaatctt
     1861 atccgcaagc cctgccacct ctccctgtgt cagtttaaag gcagtgcgcc actgcgccat
     1921 catctgaccg gattcttctg ccgtggtgtc aaaggccacg cccatcttca cggcatcgtc
     1981 agtaaactgc atcagctcgt cacgggcgat accagcctga ccacccgccg ccacaatttc
     2041 ggcgatacct tccgccgaca tgggcagttc tgttgacagg tcgcgcacct gctccgtcat
     2101 tgccttaaac gcttccggcg tatccagacc atccaccact ttccggacat ccgccatttt
     2161 tgattcaagg gcaatggcag atttgaccgg gagcgccagc gcccccagta ctgcggttcc
     2221 ggcacccgct gcgcccagtg acaggctggc aaactccttt ttaaaacctt tcagctggcg
     2281 ctgcattccc ttcagcggtg ccgatacctt atccacggca gtgatgatcg ccttcagctg
     2341 aaaactgtca gccatgcttc atctcctcgt taatgcggac ggcctcggct tccagttctg
     2401 taaactgcga aacagccacc cgtcgcagct ccagtggatt cagtttccag aaccaggcaa
     2461 cattgtagag tcgtttccgg aggtgcttcc cgtctccgac tgggtaaaaa aacgcaggat
     2521 ctgcatgctg gtcttaaaaa tatccagttt tgccatctgc gccgccgatg aacacgggat
     2581 ccccgccagc aacgggatat atttcagtgc cacctggctg tccagtttaa tactgccttc
     2641 accggaaata atgaaaggaa aacccagcgc ctcgatttcg tcatacgtgg gctcgcgcaa
     2701 ctccagcaca tgcagcattt cgttatgcgc tgtcaccggt ttttttaaaa ctatttctgt
     2761 aacgttcatt gccattactg ataccctccc tcttcgccgt ggaattcaag atctgccgtg
     2821 ccttcttcgg cattatgatt agcctctccg tgcagccatg ccgccgaaag cacatacacc
     2881 atgccgtttg ccagctcagc ggtaatcgtc atctggtctg aggtggtaat tttgtcgacc
     2941 ggaaagtttt tgggcacttt aaacgtgccc ttaacgtaag gtgaccgcca ggtctccttg
     3001 taatccacat cccctgccat gccgacgatg tcatcccgga cattggtgtt catcggcact
     3061 tcaatgccac ctgtcagcga taactgctga ccatccactt taaaaaaaca ggttccggcg
     3121 atctttgcca ttatgctgac tcctctgaat actgaagacg gaactggtta accaccgcaa
     3181 agacacgtaa ctgattaaca taatccggcg ggaacagcgt attcagtcgg ttcgggttat
     3241 ctgcatcacg ctcaactatc agatactgtt taaacagatc gtaattttcc acaataccgg
     3301 cacgctccat ctgacgatat gtcgccagaa gttccccttt gataacggca ggagtgacaa
     3361 tcgcctgccc cggaccaaaa cgggtaccat catttgccag cttgtgacgt ccgtacttgc
     3421 ttgtgatgac cgatttcagt ttgcgaagaa catatgcact ggtatgcaga gtttcactgt
     3481 ccagatagct gttgtctgcc acgccatacg cattcttttt gtaggtggtt acggaacgct
     3541 ggatctgcaa cgtgccgcct tccacataag ccgtcgccac gccgtgagat aaaagggtct
     3601 gctgctctgt catgatgaat cgcttacctt tcggtgccgg aagcatcccc accagctccc
     3661 ccgtctgtgt cggacgggca ggatcattcc ggataaatac cgcttcacgg gcaaggcgac
     3721 tggcaaccag ttcatcgaca ggcgactggg tttctttttc gtaaccggca agcgtgatat
     3781 gttgctgatt atgcatatct ccggcaccaa ccagctctga cagcgttccc agttttgcgg
     3841 tatagacatg cccgtataac tggcgcgcat aactccagcg accgctgctg tcattcattt
     3901 cggtcatcat catattgatg gaggcggcat cgttgaacgg cagaccgata aagtcgaatg
     3961 cctcatcgcc catagcagca acagcggcgg taagatcagg cgctccgcta ccggcagcac
     4021 cgacttccgt caccacccga agccctgctg gcaaaatctc accaccacca gaaccataat
     4081 aattcagaca gaccggtaac tcattaccat acagcccctt atgacgggca gtcagcgtca
     4141 ccacgcctgc atcagatgac gccgtaaacg gcagggtgat aaccccgttt accgcttcct
     4201 taatcgcggt ggcaaccgca gtggcatcat cgccattcac cacaggcacc tgtacacggg
     4261 agcgcccgac atacagactc agggtgccgc tttcctctgc ttctccggca accgtcaccc
     4321 tgaccgtcgc cgccgcccct ctggcttccg gtactgcaat aacatacagt tcaccgaaag
     4381 gatctgtctg acggtagact tccaccatac gcgccagctg gctccccgcc ccacaaatct
     4441 ggcgggcata atctgccgat ggcatcagca ccaggctgtt aacctcaatg gctgcatcgt
     4501 tgctggcatg cccgatcagt aatgcaggcg cgctggtcac cgctgtattt gccgcagaat
     4561 tgttcatctc ggcgtagaac aacggcacca gcgtattcga cggaacagta ttaaaactta
     4621 tcgtcatgat tttttagcct tatcctgttt aacacgtacc acatcacccg ccgctatacg
     4681 gcggaaccag tagctgcttt cttccacatt tcgcccttcg gaaggcaaaa ggtctccacg
     4741 ggcagggtca ggaaccgacc gccctttcag gggtttcaca aacatgaggg actcttaatt
     4801 ctgagggaaa cgcatttcca gatggtgctc aatgtcacca tccgggccag taccaggatc
     4861 gatgtaatcc acatcaatac tcagcaggga taaatccggc agggcattca cgtcctctgc
     4921 ctgtcgcgtg tcttcttccg tgatttcata cttcgccgta aaatcaaact ggtaatacag
     4981 ttcgtaacgg ttcagatcca gcagggtgcc accgacataa acaatctcgc ccccttgcgg
     5041 gtccggcatc catccaagca gggctttcca gagttctctc cggacatcat gaacggcgtc
     5101 ataggctgcc cactgccctt tttcatcacg ctcattgagg tgtactggca atagcggaca
     5161 ctaccattt
//
LOCUS       DAJTNP010000183         5084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTNP010000183 DAJTNP010000000
VERSION     DAJTNP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..131
                     /locus_tag="OO160_003960"
     CDS             <1..131
                     /locus_tag="OO160_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCR5525868.1"
                     /translation="ERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            390..574
                     /locus_tag="OO160_003961"
                     /pseudo
     CDS             390..574
                     /locus_tag="OO160_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            717..938
                     /locus_tag="OO160_003962"
                     /pseudo
     CDS             717..938
                     /locus_tag="OO160_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(959..1021)
                     /locus_tag="OO160_003963"
                     /pseudo
     CDS             complement(959..1021)
                     /locus_tag="OO160_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1471..2883
                     /gene="ospC1"
                     /locus_tag="OO160_003964"
     CDS             1471..2883
                     /gene="ospC1"
                     /locus_tag="OO160_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5525869.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2987..3137)
                     /locus_tag="OO160_003965"
                     /pseudo
     CDS             complement(2987..3137)
                     /locus_tag="OO160_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3213..4910
                     /gene="ospD3"
                     /locus_tag="OO160_003966"
     CDS             3213..4910
                     /gene="ospD3"
                     /locus_tag="OO160_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5525870.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1741 a          936 c          965 g         1442 t
ORIGIN      
        1 ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt tacgacaaaa
       61 cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt tatcaacgct
      121 tacgtaatta agggacacag cctagtctcc aggattcccg gggcggttca atcaaagcgc
      181 agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg
      241 ggaagtttgc ttctttgaga ggcgggttta cgtttacggg atttagctga acgggccata
      301 taaccacctg aaagacaatg acattgcctg tttttataac ggtaattgca gaccatgaca
      361 aaccgcagcc gtcaggctgc ctactcgagt cacaaggctc tttgtgtccg atatgccggc
      421 agcgcattac ccatgaaact ggctggaacg tacaccataa ggtgaagaaa gttatggggg
      481 cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac agttacacag
      541 ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta ccgggaaacc
      601 ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac ccgacaaaca
      661 tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt gaggaaatgg
      721 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
      781 ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg gcttatgctt
      841 ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag ttcggtaaaa
      901 gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg gcgatctacg
      961 ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac tcataaggga
     1021 agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac ctctattaaa
     1081 aaacatcagt ttttcacact tagttgagct tcatgaaaaa atcatcgagg atttcattgc
     1141 agaatacaca ttccactaac tggaagcact ccataagagg aatcagagaa agtatcgttt
     1201 ttttatagta aaattcattg ctgttcaata aaattgatat agttattaaa ttatgaaaat
     1261 aatttcacaa aaaagattaa cttgagttct taagcatcgg attttagata ttttgaaata
     1321 tctggcatca cataacatca aaagtatata atactcttat ctaattacat gcagtatatt
     1381 gtatatatac tcaaaaattg taattcatac cctgctatat cttgtaggga aagatattaa
     1441 ttaaaactgt tttcatataa ggttcatttt atgaatatat cagaaacact gaactcagca
     1501 aatacccaat gcaatataga ttctatggat aacagattac atacattgtt tccaaaagtg
     1561 acatcagtgc gaaacgctgc acaacaaact atgccagatg aaaaaaattt aaaagatagt
     1621 gcaaatatta ttaaaagttt ctttaggaaa actatagcag cacagagtta tagtagaatg
     1681 ttctctcaag gctctaactt taaatcttta aatatagcaa ttgatgcacc atcagacgct
     1741 aaagcctcat ttaaggctat tgagcacctt gacagattat cgaagcatta tatatctgaa
     1801 ataagggaaa aacttcatcc tctttctgca gaggaactca atttgctttc gctaattatt
     1861 aattctgatt taatcttcag acatcaaagt aattctgatt tgtctgataa aattttaaac
     1921 attaagtcat tcaataaaat tcagtctgaa ggaatatgca caaaacgaaa cacatacgct
     1981 gatgatataa aaaaaatagc taatcatgac tttgtgtttt ttggcgttga aatctctaac
     2041 catcagaaaa aacaccccct gaatacaaaa catcacactg ttgattttgg tgcaaatgcg
     2101 tatatcattg atcatgactc tccatatgga tatatgacat taaccgatca ctttgataat
     2161 gctattccac ctgtttttta ccatgagcac caatcatttt tagataaatt ttcagaggtt
     2221 aataaagaag ttagtcgata cgtacatgga agtaaaggaa ttatagatgt accaatattc
     2281 aatactaaag atatgaagtt agggctcgga ttatacctga ttgactttat tagaaaaagt
     2341 gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc ttgcccctgt ggatctggat
     2401 agaatcataa actttgtttt tcagccagag taccatatac ctaggatggt aagtacagaa
     2461 aacttcaaaa aagttaagat tagagaaata tccttagagg aggctgttac agcatctaat
     2521 tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg ctctccaggc tctttttctt
     2581 tcgattacta atcaaaaaga ggatgtcgcc ttatatatat tatctaattt tgagataact
     2641 agacaagatg ttatttccat aaagcatgag ttgtatgata ttgagtatct acttagcgct
     2701 cataattcaa gctgtaaagt acttgagtat tttatcaata agggattggt tgatgtaaac
     2761 acaaagttca aaaaaactaa tagtggggat tgtatgttgg ataacgcaat aaaatatgag
     2821 aatgcagaaa tgataaaact attattgaaa tatggtgcaa catctgacaa taaatatatt
     2881 taatcaaaat tgaatatcgt ttagcaaaag gcagtatttg cacaaaatat cgatatattg
     2941 tttattgtca gtatggctca atgtgataat agttggaaag tttgatgggt ttcgccccgt
     3001 tgtagcggta gtcgaactgg aaggtcttca ggcactgctt acagcgatag agcagccccc
     3061 cctgaactgg aatggccgtt ccggataccc ccctgagttt cagagtaacg gtgacaaacc
     3121 acatcaatct ttgccatcaa tcatccaaag ggcaaagagt acaacaacac taagtctgcg
     3181 tcacaaccca tcaatgaaag gaatatatac atatgccatc agtaaattta atcccatcaa
     3241 ggaaaatatg tttgcaaaat atgataaata aagacaacgt ctctgttgag acaatccagt
     3301 ctctattgca ctcaaaacaa ttgccatatt tttctgacaa gaggagtttt ttattaaatc
     3361 taaattgcca agttaccgat cactctggaa gacttattgt ctgtcgacat ttagcttcct
     3421 actggatagc acagtttaac aaaagtagtg gtcacgtgga ttatcatcac tttgcttttc
     3481 cggatgaaat taaaaattat gtttcagtga gtgaagaaga aaaggctatt aatgtgcctg
     3541 ctattattta ttttgttgaa aacggttcat ggggagatat tattttttat attttcaatg
     3601 aaatgatttt tcattccgaa aaaagcagag cactagaaat aagtacatca aatcacaata
     3661 tggcattagg cttgaagatt aaagaaacta aaaatggggg ggattttgtc attcagcttt
     3721 atgatcccaa ccatacagca actcatttac gagcagagtt taacaaattt aacttagcta
     3781 aaataaaaaa actgactgta gataattttc ttgatgaaaa acatcagaaa tgttatggtc
     3841 ttatatccga cggtatgtct atatttgtgg acagacatac tccaacaagc atgtcctcca
     3901 taatcagatg gcctgataat ttacttcacc ccaaagttat ttatcacgcg atgcgtatgg
     3961 gattgactga gctaatccaa aaagtaacaa gagtcgtaca actatctgac ctttcagaca
     4021 atacgttaga attacttttg gcagccaaaa atgacgatgg tttgtcagga ttgcttttag
     4081 ctttacaaaa tgggcattca gatacaatct tagcatacgg agaactcttg gaaacttctg
     4141 gacttaacct tgataaaacg gtagaactac taactgcgga aggaatggga ggacgaatat
     4201 cgggtttatc ccaagcactt caaaatgggc atgcagaaac tatcaaaaca tacggaaggc
     4261 ttctcaagaa gagagcaata aatatcgaat acaataagct gaaaaatttg ctgaccgctt
     4321 attattatga tgaagtacac agacagatac ccggactaat gtttgctctt caaaatggac
     4381 atgcagatgc tatacgcgca tacggtgagc tcattcttag cccccctctc ctcaactcag
     4441 aggatattgt aaatttgctg gcctcaagga gatatgacaa tgttcccgga cttctgttag
     4501 cattgaataa tggacaggct gatgcaatct tagcttatgg tgatatcttg aatgaggcaa
     4561 aacttaactt ggataaaaaa gcagagctgt tagaagcgaa agattctaat ggtttatctg
     4621 gattgtttgt agccttgcat aatggatgtg tagaaacaat tattgcttat gggaaaatac
     4681 ttcacactgc agaccttact ccacatcagg catcaaaatt actggcagca gaaggcccaa
     4741 atggggtatc tggattaatt atagcttttc aaaataggaa ttttgaggca ataaaaactt
     4801 atatggaaat aataaaaaat gaaaatatta cacctgaaga aatagcagaa cacttggaca
     4861 aaaaaaatgg aagtgatttt ctagaaatta tgaagaatat aaaaagctga atattatctc
     4921 gtggtgatac actgaatttg aggtaaccgg ctcatttaaa ccgtctggtc tgttacctcc
     4981 ggttccacaa aaataatgtc catcattttg aagtggacac tatcgtatgg aataccggac
     5041 ctgaatcact gaagctttac gccttcattt cgaagaacat ttac
//
LOCUS       DAJTNP010000184         5071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTNP010000184 DAJTNP010000000
VERSION     DAJTNP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(294..539)
                     /locus_tag="OO160_003967"
     CDS             complement(294..539)
                     /locus_tag="OO160_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525871.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1316..2158)
                     /locus_tag="OO160_003968"
     CDS             complement(1316..2158)
                     /locus_tag="OO160_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5525872.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2243..2440)
                     /locus_tag="OO160_003969"
     CDS             complement(2243..2440)
                     /locus_tag="OO160_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525873.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2460..2948)
                     /locus_tag="OO160_003970"
     CDS             complement(2460..2948)
                     /locus_tag="OO160_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525874.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2945..3322)
                     /locus_tag="OO160_003971"
     CDS             complement(2945..3322)
                     /locus_tag="OO160_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5525875.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3369..3746)
                     /gene="cbtA"
                     /locus_tag="OO160_003972"
     CDS             complement(3369..3746)
                     /gene="cbtA"
                     /locus_tag="OO160_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5525876.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3826..4047)
                     /locus_tag="OO160_003973"
     CDS             complement(3826..4047)
                     /locus_tag="OO160_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5525877.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4134..4610)
                     /locus_tag="OO160_003974"
     CDS             complement(4134..4610)
                     /locus_tag="OO160_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5525878.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4626..>5071)
                     /locus_tag="OO160_003975"
     CDS             complement(4626..>5071)
                     /locus_tag="OO160_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5525879.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1178 a         1235 c         1342 g         1316 t
ORIGIN      
        1 atctactgat tgcgctaaca aattcaatga gatcacctct atgcctgccc gttaagtatg
       61 tcaggctttt ttccgtaatg ctctaacttc cacgcattgg tttctgacat caaataaggc
      121 tatgactttc tctccctttt aatctcattc tgatctaaaa ccatatctta aaaaaatgca
      181 tgggtatttt aattattttg atgttgatct aacgcactca aggaccattt tattaccgat
      241 tatccattct atttaactgt tgcgatataa atgtttgttt taggtgcttt aattcagtac
      301 aagtcatcaa accgtaccag accctgactg tcagtgacac cgacaggtgg gggcggcggt
      361 gtggggtcaa actgagtgtg tctgcctctg gcccacagag ccagctctgc cgccattgcc
      421 cgtggatgat actccagctg cacgttgaga ccggaaactg ggttgttgtt ctcatccacg
      481 aactgcagcg ccagccagtg catccgctgc ttaccgcagg cgacttcttt gtacttcatt
      541 ttgtgactca ggcaggtgac tgtcgtgttg cctgttgcgt ccattttgcg gttaaaccct
      601 tttaattcaa tttgttaaat tgctttccac cttttgcggg gcgcgactta accaaaatat
      661 tcactgttat cataatatgc aatgggctgg aggttgcagg tcatttaggg attttctgta
      721 agccccgttt tgcagaacgt tctatctccg attgagatcc tgtctctcgg tattaccggt
      781 agtctggcca taatccccta tggatcatat tgatatagta ggcataacac ccaaaagttg
      841 actaaagcta aatcaatcag agtgaaaaat gttcggtttg atgttggggg ggactgagga
      901 ggggccaacg caggtggtat gcgttttact tcagaccgga caaagaccgg atgtatccgt
      961 gcagtaatga atccgttagc caggtgctca tgtggaaata gtgtctattg gggatttgaa
     1021 gtttgacaga aaaggccaca gtgaccagaa gggggctact tagaagaaat gttcaggctg
     1081 ggtggaagag gtaaagcagt gacatcagca ttcaaagtga ccggatatct accagaaccc
     1141 cagaaagcaa aaaaccagcc cgtaggctgg ttcttctaaa tagtggtgcc ggactcggaa
     1201 tcgaaaattt cttttatcat attgatatta atgcaatttg tttggtttga ttaaaaatgt
     1261 accctcaatt atacccgcag tcccttttga tatcgaaagg gaatcactct cgggatcagg
     1321 ctgagagcac agaaggaaag tacctggcta ttatatcgtt aattctgtca atcagttcca
     1381 gccttttgaa agtgatgtgt gcagatccct tcttaaagta tttaatgatg aacatctcgt
     1441 cttcatagcg ctctttaccc tgaacggact ggatgtgacc atccagacga atagtgatgt
     1501 tttcccggtt gtcggggatc ggtttgccgc tgaacagatg tagcattctt tccaggtcga
     1561 cgagtcggtc agtctgttgc ccggtgataa gatgaaaccc ccaccggtcc cacctcacca
     1621 ggttgttgac gataatttta ctgccaaatt tacagggtga gttggttttg taatcccagc
     1681 tcagcccttt gaagacgttg atcactcccc gttcaaacac ttcatctttg ttctggtgca
     1741 gttgttcaaa tgtgctcaga atgttcgctt cgctgatttc cggaaaatta tcatattcca
     1801 gtgacctgta ccatgtgtcc cgggtctgcg catccatcag actcagcatc ccggaacgtt
     1861 gcatcagatc acgccagagt tcgcgatcaa gattgcgtgt gatggctttc attgcggttt
     1921 ccggtttctc catcagccag caaccgcagc gaaaatcctg tttcatggcc cagtccagag
     1981 ccgttttacc tctaatactt tctgtcattg ctgatacttc agccagttgt cggataagtg
     2041 tgtcgatttg cagcaatgta ctctttcgta cagccagaat gtggcggata ctggttgagc
     2101 agataacgtc cgtatgcccg ttgaccactt ccggacgaga ggctgtgatt tcagacataa
     2161 tcgatattcc tttttgcaga taaacgaaaa cgccagccgt attcaggctg gcgtcagtgg
     2221 aatgaagtct ggtgagtaag actcaccggt tctgttgcag aaactgcagc tgacgcaggg
     2281 tacggatatc ttccctggcc tgttcaatac agggcagcag tgctgccgaa tccgttttat
     2341 cctcatcgtt gtcgaagata atccctgatt cgcagtcaat attgtcctgc agccacgcaa
     2401 tcagaatatc cagcgccgtt tctgtggtta ataatttcat ccagtgtctt ttgtcctgat
     2461 taccagtcga aagtggggaa ccccggcaga cattctgcgc cggcgtccag atgaatgaga
     2521 ctgacgccat aacgccgggt gagagtgacg accagacgac ggaacgttct ggacaggccc
     2581 aggcgtttaa gccgcagaac cgggtacgac catgcatcgg tacgtaacag atagccgctg
     2641 cctgtaaaat gaatccattc tgtttcgccg tcgtcattag tctggtgtga cagcgagtac
     2701 agccaggcgt tgtccgtttc cgtgatatgt gcggtgctgc aacgtatgca ggtaagggtt
     2761 gttaacggtg gtggtgtact ggttgcgtgt tggtcggatt tatcgactac gcggagtgaa
     2821 taaccgtttt cagagacctt gttaatcagt tcactgagat taacaccatc gacgtcaacg
     2881 acaatgcgcc ccatgttcag tgcctgcacg ttaacgctgt cggcttccag cgtcagggcc
     2941 agtttcattg tttcgcttcc ggatgcttcc cccgggtaat gttatttacc gttctgtagt
     3001 tatcgcgggt catcaggccg gttgcccggc gtgcccggag aatatcaatg ctgttgatta
     3061 actgcgacgg ggctcctgcg ctgaatcccg gctggtcggt acgtaccagc gcgtattttt
     3121 caacgagaaa gttcaccgcg tcacacagtg aaattcctgc ctcaatatgc tgctcaatca
     3181 cacgttcatc agcgaacggt gtgtcattca gcgtgaggcc atagtgctgg tccagcagtc
     3241 gggtgagcag tgtctgccag atggtgatgg gagacgggca gcccgatgcc tcccgtacgt
     3301 gagtgtcggg taatgtattc atcttaagtt ctctgtaaca ggtggttata aaggggagat
     3361 atccggtgtt atacggtggt tgcgggcgct gccggtgtcg gataaacagc cagataaacg
     3421 taaccacagg agccgagggt gtcggcttcg caggtcagcc ctttcgcata cagtgtgacg
     3481 gtatgctgat ggcggggatt cagttcaccg ccagtgagca tgagttccag ttgtttcatc
     3541 agcagcggaa aggcctggtc caggtggtac gcatctacgt cgctgaacct gcctctgata
     3601 ccggcgcggt ctgcaaggta atgcaggcgg ttaccctcct gtaccagtcg ggccccgaaa
     3661 cagggcgtca cggtgcaggg cagcccccac caggggcggt cgtgattgtc gtcgggaggc
     3721 gttgtcccgg ggagtgtgtc tgacacgata aagtcctcac agaaaatcgg ttaagaatgg
     3781 tccggtagtg gcgataattc tgctcatcag aattcccttc cagactcagt tcagggtgac
     3841 gctcatcagc cggacatacg gaccaaaact gtccttacgg cgttctgcaa acacagccag
     3901 tacaccggga atatcctgca cttcacggcc ggtatatgta tccgtgctac cgtgccagcg
     3961 gtatttaccg gtacagaacg gaaaaagacg ggatgcagga tgctgacggt gaatacgcat
     4021 ggcttcacca cgggtgatga ttttcatgag aatagtcctc ctgaatgtga aagtaagaaa
     4081 cagctgagaa agagaaactg aagtgatatg aaggtgattg tgacggtgac gggttaaagc
     4141 agaccgtgtt ccgcaaagga gaaaacctgg ctgccaccga ctatcagatg gtccggtacc
     4201 cggatatcaa ccagggccag tgcctgtacc agacgctcag tgataaggcg gtctgccttg
     4261 ctggggggga cttcaccgga cgggtgattg tgcgccagca ccacggcagc ggcattgtgg
     4321 tacagggcgc gtttaatcac ttcccgggga tggacttccg tacggttgat ggtgccggtg
     4381 aagagggttt caccggcaat cagctgattc tggttgttca gatacagcac ccggaactct
     4441 tcacgctcca gtcccgccat gttcagaatc agccattccc gtgccgcatg cgtggaggtg
     4501 aaggccacgc cgggttcatg aagatggcgg tccagggttt tcagggcccg cagaatgagg
     4561 ctgcgctctc tggttgtcat ttctccgggc agaaaggaaa gctgctgcat tgtccctgcc
     4621 accattcagt cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc
     4681 cgcagccggt aataatggac cgtcatggca taacactccg tacgacaggc atgatgactg
     4741 tacgtcatca gacaggcagc aatgccggca gcttccggac tcatttcagc gcggttaccg
     4801 ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc gggtgccata
     4861 aatgccccgc cgttgttcag ggtgtacaga ttccagatac caccgcagta gtcttcgcac
     4921 agacggtcca tccagccgaa gacacggggc tccagggtca cccactgtgg aatgaggcca
     4981 aagtgctgcg gccagaagct gatgcgctgt tcatcaggga cgagggtggc aaccagttgt
     5041 tgcaggtcag tctccggtgc acaggccgaa g
//
LOCUS       DAJTNP010000185         5015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTNP010000185 DAJTNP010000000
VERSION     DAJTNP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..549
                     /locus_tag="OO160_003976"
     CDS             <1..549
                     /locus_tag="OO160_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5525880.1"
                     /translation="IIGRGCGGLGAITTGKDSQGKLPPAVYWLTLPWRIGMWLSMRWF
                     CRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPMLDLVVP
                     EEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEAIIFIR
                     ARRSHIVLKEEHKAMLKLWENR"
     gene            complement(603..1208)
                     /gene="azoR"
                     /locus_tag="OO160_003977"
     CDS             complement(603..1208)
                     /gene="azoR"
                     /locus_tag="OO160_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5525881.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1409..>5015
                     /gene="hrpA"
                     /locus_tag="OO160_003978"
     CDS             1409..>5015
                     /gene="hrpA"
                     /locus_tag="OO160_003978"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5525882.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNG"
BASE COUNT         1221 a         1253 c         1402 g         1139 t
ORIGIN      
        1 attattggcc gtgggtgcgg tgggcttggc gcgataacaa cagggaaaga tagtcagggg
       61 aaactcccgc ccgccgttta ctggctgaca ttgccctggc gcatcgggat gtggctatct
      121 atgcgttggt tttgtcgtcg cctggagccg gtgagcaaaa tgactgctgg tgtttattta
      181 agggcgtttc cacgacatat tccggcacag aatgcggttc tggacgtcac ctttgaattc
      241 cctcgtggac gagcgacaaa agatcgactc tatttctgtg taccgatgtt ggatctggtg
      301 gttccggaag agggggagct ccgacaggcc gtggcgatgc tggaaacatt acgcgaagag
      361 caaggcagcg ttctggtcca ttgcgcattg ggattatcgc gcagtgcgct ggtagtggcg
      421 gcatggctgt tatgttacgg acactgtaaa acagttgatg aagcgattat ctttattcga
      481 gccagacgct cgcatattgt gcttaaggaa gagcacaaag cgatgttgaa attatgggaa
      541 aacaggtaag tggattgaga tgtggactga atatctacag tccacatcaa gaccgtgtcc
      601 ggttatgcag caacaatgct gtcgatggct gcttttgcgt cagactgtgc tttcgctgcc
      661 atttccggac cgtatgcgat cccttcggcg aagacaaatt tcacatcggt aatgccgata
      721 aagccgagga acgtggacag atacggcgtc accaggtccg ttggtccatc tttgtggatc
      781 ccaccgcggc tggtaataac gatggctttt ttacccgtta ccagaccttc cggaccgttc
      841 tcggtatagc ggaaagtaac gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga
      901 gttgagatgt tgaagttata catcggtgcc gcaataacga taacgtcgtg ggctttcagc
      961 tcggcaatca gctcatcgga aagcgccaga gcttcctgct gacgcggagt cagcggcgca
     1021 tcgctcggac gcagagcgcc aaccagttcg ccatccagta ccggaatcgg atttgcagct
     1081 aggtcgcgaa cggtgatttc atcagcggag tgcttttcgc tccattgttc aacaaaataa
     1141 tcggacaact gattagactg agagtaccct gccaggatgc tggatttaag aactaatacc
     1201 ttgctcatgg tgtttcctta tagatgtttg aatggacgat gccccgttgc ttgctgacac
     1261 tttattcaca atcctgccac agagatcgcg caataaatcg aagtctatgt tcgaatttat
     1321 tgaacaacgc atagaaagcc gcgatgtggt actctatatc tatcatttaa aagaaaatta
     1381 atcaggcaga ctactgccca ctaacgttat gacagaacaa caaaaattga cctttactgc
     1441 cttgcaacag cggctggatt cgctgatgct gcgcgacaga ctgcgttttt ctcgccgtct
     1501 gcattgtgtt aagaaggtta aaaatcctga tgcacaacag gccattttcc aggagatggc
     1561 gaaagagatt gaccaggcgg cagggaaagt cctgctgcgt gaagcggcac gaccggaaat
     1621 cacttatcct gacaatttac cggttagtca gaaaaaacag gacatactcg aagcgattcg
     1681 tgatcaccag gtggtgatcg tcgccgggga aaccggctcc ggtaaaacga ctcagctacc
     1741 gaaaatctgt atggagctgg ggcgcggtat taaagggctg atcggtcata cccagccgcg
     1801 tcgcctggcg gcacgaacgg tggccaaccg tattgcggaa gagctgaaaa cggagccggg
     1861 cggttgcatc ggttacaaag tgcgtttcag cgatcacgta agtgataaca cgatggtcaa
     1921 gctgatgacc gacgggatcc tgctggcgga gatccagcaa gaccgcctgc tgatgcagta
     1981 cgacaccatc attattgacg aagcgcacga acgcagcctg aatatcgatt ttctgctcgg
     2041 ctatttgaaa gagttgctgc cgcggcgtcc tgacctaaaa atcattatca cttccgcgac
     2101 tatcgacccg gaacgctttt cgcgccactt taataatgcg ccgattattg aagtctccgg
     2161 tcggacctat ccggtggaag tgcgctatcg cccgattgtt gaagaagccg atgacaccga
     2221 gcgcgatcag ttgcaggcga tttttgacgc cgtagacgaa ctaagccagg aaagccccgg
     2281 cgacattctg atctttatga gcggcgagcg ggaaattcgc gataccgccg atgcgctgaa
     2341 caagctgaac ttacgccata ccgaaatctt gccgctttat gcgcggcttt cgaacagcga
     2401 acagaaccgc gtgttccagt cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc
     2461 ggaaacctcg ctgaccgtac cggggattaa atacgtcatc gaccccggta cagcgcgtat
     2521 cagccgctac agctatcgca ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc
     2581 gtctgccaat cagcgtaaag gccgctgtgg tcgtgtgtcc gaagggatct gtattcgtct
     2641 ctattccgaa gacgatttcc tctcgcgccc ggagtttacc gatccggaga ttctgcgtac
     2701 caacctggcc tcggttattt tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt
     2761 cccgtttgtc gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga
     2821 actgggcgcg atcaccacgg atgaacaggc cagcgcctat aaactgacgc cgctcggtcg
     2881 ccagctctcg cagttgcctg tcgacccacg tctggcgcgt atggtgctgg aagcgcaaaa
     2941 acatggctgc gtgcgtgagg cgatgattat cacgtccgcg ctctccatcc aggatccgcg
     3001 cgaacgtccg atggacaaac agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa
     3061 agaatccgac ttcctcgcgt ttgtaaatct gtggaattat cttggcgagc agcaaaaggc
     3121 gctttcttcc aacgccttcc gtcgcctgtg tcgtaccgat tacctcaact atctgcgcgt
     3181 gcgcgaatgg caggatatct acacccagtt gcgccaggtg gtgaaagaac ttggcattcc
     3241 ggtcaacagc gaaccggcgg agtatcgtga aattcacatc gccttactga ccggtttgct
     3301 ttcccatatc ggcatgaaag atgccgataa acaggaatat accggtgcac gtaacgcgcg
     3361 tttctccatc ttccccggtt ctggtttatt caaaaagccg ccgaaatggg taatggtggc
     3421 ggaactggta gaaaccagcc gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg
     3481 ggtagaaccc gttgctcagc atttgattaa acgcacctac agcgaaccgc actgggaacg
     3541 ggcgcagggc gcggtgatgg caacggaaaa agtcactgtt tatggtttgc cgattgttgc
     3601 tgcgcgcaag gtcaactaca gccagatcga ttcggcgtta tgtcgtgaac tctttattcg
     3661 ccacgctctg gtggaaggtg actggcagac gcgtcacgca ttcttccgtg aaaacctgaa
     3721 actgcgggcc gaagtggaag agctggaaca caaatcacgt cgccgcgata ttctggttga
     3781 tgacgaaacg ctgtttgagt tctacgacca gcgcatcagc cacgatgtaa tctccgctcg
     3841 tcacttcgat agctggtgga aaaaagtcag ccgcgaaacg cctgatttgc tcaactttga
     3901 aaagagcatg ttgatcaaag aaggggcgga aaaaatcagc aagctggatt acccgaactt
     3961 ctggcatcag ggcaatctca agctgcgttt gagctatcag tttgagccag gcgcggacgc
     4021 ggacggtgtg accgtacata ttccgctgcc gttacttaac caggttgagg aaagcgggtt
     4081 tgaatggcag atccccggtc tgcgccgcga attggtgatt gctctgatta aatcgttgcc
     4141 gaaaccggta cgccgtaatt ttgtgcctgc accaaactac gccgaagcgt ttttaggccg
     4201 cgtcacaccg ctggagttac cgttgctcga cagccttgag cgcgagttac ggcggatgac
     4261 cggcgttacc gttgaccgcg aagactggca ctgggatcag gtgcccgatc acctgaaaat
     4321 caccttccgc gtggtggatg acaaaaacaa gaagctaaaa gaagggcgct cgctacaaga
     4381 tctgaaagat gcgctgaaag gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg
     4441 tatcgagcag agcggcttac atatctggag ttttggtcag ctgccggaaa gttacgaaca
     4501 gaagcgtggc aactataaag tgaaggcgtg gccggcgctg gtggatgaac gcgacagcgt
     4561 gacgatcaaa ctgtttgata acccgctgga gcaaaaacag gcaatgtgga atggtcttcg
     4621 ccgtctactg ctgctaaata tttcctcgcc gatcaagtat ttacatgaga agttaccaaa
     4681 caaagccaag ctggggctgt actttaaccc gtatggcaaa gtgctggagc tgatcgacga
     4741 ctgtatctcc tgcggtgtgg atcaactgat cgacgccaat ggtggcccgg tctggacgga
     4801 agaaggcttt gctgcgctgc atgaaaaagt gcgcgccgaa ctgaatgaca cggtggtgga
     4861 tattgcgaag caggtcgagc aaatcctcac tgcggtgttc aatatcaaca aacgtctgaa
     4921 agggcgggtg gatatgacca tggcactggg gctttctgat attaaagcgc agatgggcgg
     4981 gttggtatat cgcggttttg tcactggtaa cggct
//
LOCUS       DAJTNP010000186         5012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTNP010000186 DAJTNP010000000
VERSION     DAJTNP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO160_003979"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO160_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5525883.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO160_003980"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO160_003980"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5525884.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO160_003981"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO160_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5525885.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO160_003982"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO160_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5525886.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5012
                     /gene="yidZ"
                     /locus_tag="OO160_003983"
     CDS             4378..>5012
                     /gene="yidZ"
                     /locus_tag="OO160_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5525887.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWE"
BASE COUNT         1141 a         1204 c         1252 g         1415 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa ca
//
LOCUS       DAJTNP010000187         4952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTNP010000187 DAJTNP010000000
VERSION     DAJTNP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..515
                     /locus_tag="OO160_003984"
     CDS             <1..515
                     /locus_tag="OO160_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5525888.1"
                     /translation="VGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNIISNNSV
                     VMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANT
                     APDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQ
                     SVIGVTFIYE"
     gene            849..3128
                     /gene="ydeP"
                     /locus_tag="OO160_003985"
     CDS             849..3128
                     /gene="ydeP"
                     /locus_tag="OO160_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5525889.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3376..3579
                     /gene="safA"
                     /locus_tag="OO160_003986"
     CDS             3376..3579
                     /gene="safA"
                     /locus_tag="OO160_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5525890.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3647..4408
                     /gene="ydeO"
                     /locus_tag="OO160_003987"
     CDS             3647..4408
                     /gene="ydeO"
                     /locus_tag="OO160_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5525891.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4810..>4952
                     /locus_tag="OO160_003988"
     CDS             4810..>4952
                     /locus_tag="OO160_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5525892.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1414 a         1048 c         1110 g         1380 t
ORIGIN      
        1 cagttggggc ggccggtgga gttgtcataa aagcggggga agtcattgcc cgtattcata
       61 tgtataaaat tgcaacatta ggaagcggaa atccgcgtaa ttttacgtgg aatattattt
      121 caaataacag tgttgttatg ccaacaggcg gctgcaccgt tgatagtcgt aatgtcaccg
      181 tggatctccc ggactttcct ggaagcgcag aaatacccct tggcgtatat tgctcaagcg
      241 aacaaaagct ctcattttat ctttcgggtg ccaccactga cagtagccga caagtgtttg
      301 ctaacacagc acctgatgca acaaaagcat cgggagtggg tgtcactcta atgcgtaatg
      361 gaaaaatact ggctacggga gaaaatgtct ccctggggac agtaaataaa agcaaagtac
      421 ctctgggttt aagcgcaacc tatggacaaa caggaaacaa ggtttcagcc ggaaccgtcc
      481 agtcagtcat tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc
      541 gctccattca cgaaaattgc gcggataatt attttgtgaa ggctattatc ctacacctgt
      601 aagaaaatcc gcgcacaatt gaagccagag tcttattttt tatattggaa aatatgttaa
      661 tttaaacact atgagagatg atgcttaact tatcgtattt aatctattgt ttaacgataa
      721 tagattaaaa atagaaagag agtagtccct tactcttata tatccatgtt ggcgataatc
      781 cctttgtatg tactgtgcat catcgctatt acaaatccta ataattcatt tccacacagg
      841 ataagtagat gaagaaaaaa attgaatcct accagggtgc tgcaggtggt tggggtgctg
      901 ttaaatccgt agcgaatgca gtacgtaagc agatggatat acgccatgat gttattgcca
      961 tgtttgacat gaataagcca gagggctttg actgtccggg ttgtgcatgg ccagatccta
     1021 agaacagtgc gtcattcgac atttgtgaaa acggcgcaaa agcaatcgct tgggaagtca
     1081 cggataagca ggtaaatgcc tctttctttg ctgagaatac ggttcaatca ttacttacct
     1141 ggggagatca cgagcttgag gctgcggggc gtctcactca gcctttgaaa tatgatgccg
     1201 tcagcgactg ttacaagcca ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc
     1261 ttcaaagcta tagtgatccc aatcaggttg aattctatac ttcgggccgc acttccaatg
     1321 aagctgcctt tctttatcag ctttttgccc gtgaatacgg gagcaataac tttcccgact
     1381 gctccaacat gtgccatgaa ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta
     1441 aagggaccgt gttgctggaa gactttgaga agtgcgattt agtcatttgc attgggcata
     1501 accctggtac aaaccaccct cgcatgctga cttcgttgcg cgctttagtg aaacggggag
     1561 cgaaaatgat cgccatcaat cctctacagg aacgtggcct ggagcgattt accgcaccgc
     1621 aaaacccgtt tgaaatgctg acgaactctg agactcagtt ggccagtgcc tactacaacg
     1681 tgcgcattgg tggcgatatg gcgttgctca aggggatgat gcgcctgtta attgagcgcg
     1741 atgatgctgc aagtgccgca ggtcggccct cattgcttga tgacgaattt attcaaacgc
     1801 ataccgtcgg ctttgacgag ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg
     1861 aacgtatttc tggactaagt cagacacaaa tcgccgaact ggctgacgca tatgccgctg
     1921 ccgaacgcac cattatctgt tacggaatgg ggatcactca gcacgaacat ggtacccaga
     1981 acgtgcagca actggtcaat ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg
     2041 gtatctgccc actacgtggt cactctaatg tacagggcga ccgaaccgtc ggtatcaccg
     2101 agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc cccccacctc
     2161 atgcacctgg acatgctgca attgccagca tgcaagcaat atgtacgggg caggctcgag
     2221 cattgatctg catggggggc aactttgcac tggcaatgcc agatcgggaa gcgagcgctg
     2281 taccgttaac gcaattagat ttggcggtac acgtagccac taagcttaac cgctctcatc
     2341 tgctgaccgc acggcatagc tatattctgc cggttctggg gcgtagcgag attgatatgc
     2401 aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat gtcgatgatt catgcctcgc
     2461 gtggcgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa
     2521 tcgcgcaggc agcactaccc cagagcgtgg tagcctggga gtatctggtg gaagattatg
     2581 atcgcattcg caatgacatt gaagctgtgc tgccagagtt cgccgactat aaccagcgca
     2641 tccgtcatcc cggtggtttt cacctgataa atgcagctgc tgaaaggcgc tggatgacgc
     2701 cgtcaggtaa ggctaatttc attaccagca aagggctgtt agaagatccc tcttcagcgt
     2761 ttaacagtaa gctggtcatg gcgacagtac gcagccacga tcagtacaac acgacgattt
     2821 atggtatgga tgatcgctat cgaggggtat tcggtcaacg agatgtggtc tttatgagtg
     2881 ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt attgcgctta
     2941 cgccagacgg taagcgcagc tcacgccgca tggatagatt aaaagtggtc atttacccta
     3001 tggctgaccg ctcactggtg acctattttc cagaatcgaa tcacatgcta acacttgata
     3061 accacgatcc attaagtggt attcctggct ataaaagtat tccggttgaa ttagaaccat
     3121 caaattaatg cctcttctca tttcttctgc tgtcatccgc acagcagaag aattcctcat
     3181 tgactattat tgcgcaattt gctcacatgg attaaattaa actacatact ataagatata
     3241 aacttctgcc tacagctgta agaaactccg ctcagtactg aagcaccagt cctatttctt
     3301 cttttctcca gcctgttata ttaagcatac tgattaacga cttttaacgt tatccgctaa
     3361 ataaacatat ttgaaatgca tgcgaccaca gtgaaaaaca aaatcacgca aagagataac
     3421 tataaagaaa tcatgtctgt aattgtggtt gtcttattac tgacacttac gttgatagcc
     3481 attttttcgg caattgatca gctgagtatt tcagaaatgg gtcgcattgc aagagatctt
     3541 acacatttca ttatcaatag tttgcaagac tggaaataaa cagcaaatta taaatgtgaa
     3601 atgtttaaaa ggtatcgata aaactttatt gttttaagga gataaaatgt cgctcgtttg
     3661 ttctgttata tttattcatc atgctttcaa cgctaacatt ttagataaag attacgcctt
     3721 ctccgacggc gagatcctga tggtagataa cgctgttcgt acacattttg aaccttatga
     3781 gcggcatttt aaagaaatcg gatttaatga aaataccatt aaaaaatatc tacaatgcac
     3841 taacatccag acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc
     3901 gactggattg cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa
     3961 tttttcagaa cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat
     4021 tacactatta actaacggtg tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat
     4081 gaagctggcg cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag
     4141 cctgttgaag aaaaaactaa agcaagagca aacgacattc tcacagattc tattagatgc
     4201 cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata aaattgccga
     4261 acaatgtggt tatgccagta catcttattt tatttatgcg ttccgcaaac atttcggcaa
     4321 cagtccgaag agagtttcta aggagtaccg ttgtcaaagg cacacgggta tgaatacggg
     4381 caacacgatg agtgctttag ctatttgatt atttgctaac gagtagtcaa ccacgcacgc
     4441 tacgtaagaa ttatatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatgtt
     4501 tattgaaata aataagtgac atccatcaca tatttatgca cttgcataac ctgttgcatg
     4561 attatttatg atctcaattc ggcattttgt cagtaaaatg caataattta ttaaatatca
     4621 ataaattagt tgtttatcgg cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct
     4681 ttatggactg ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt
     4741 cacttacatc cacttatttc tcttcgtaaa attacttcgg aattaagtac aataagaaga
     4801 ggaacattta tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata
     4861 ctggcatctg gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca
     4921 accaaaacaa acgttgcttt ctcagacttt ac
//
LOCUS       DAJTNP010000188         4856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTNP010000188 DAJTNP010000000
VERSION     DAJTNP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1308)
                     /locus_tag="OO160_003989"
     CDS             complement(<1..1308)
                     /locus_tag="OO160_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5525893.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIL"
     gene            complement(1324..2208)
                     /locus_tag="OO160_003990"
     CDS             complement(1324..2208)
                     /locus_tag="OO160_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5525894.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2219..2824)
                     /locus_tag="OO160_003991"
     CDS             complement(2219..2824)
                     /locus_tag="OO160_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5525895.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2995..>4856)
                     /locus_tag="OO160_003992"
     CDS             complement(2995..>4856)
                     /locus_tag="OO160_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR5525896.1"
                     /translation="TIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKK
                     ALMIINPDAYYWGLINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQEL
                     QMLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMP
                     DNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIV
                     MTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLAND
                     SSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDN
                     LQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMK
                     QKIWQRALSHRKNNPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDF
                     LESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDA
                     LAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNP
                     RFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1413 a         1204 c         1008 g         1231 t
ORIGIN      
        1 caaaatatcg cttacggcag gctcgtttgc cagcgcatcc agtacatagc gtccatgccc
       61 tgcggcaatg tcaaccacgc ggacggctaa acctttggcg tggagatcgg caacggcttg
      121 tttaatcaat atttgcaaat gggttttgcg ctggcgaata ccgcgccatc caacactgtt
      181 caggtagatt ttgtcgatta aacgcccgaa tgcgttactg ccttgcggtt gattttgata
      241 gacataatcc agggaactgc ctgaatcaaa gccggtactc attccgagat gcaggcccgc
      301 agagtgcgca ccgaacaatt tcatcgcctt tcgcataaag cgatacgcta aatcaaccgg
      361 cgataatggc acgggtccac cagaaaggag ccgccatcga tccgctgatg gtcctgtgcg
      421 gtcttcatgc tgataatcaa atttttgcga tttgttagca tataagcggc agataaagct
      481 ttgcattttt tcaaatgcct gcgccctgtt ctcttcaccc aatgtgtcgt gataaaagcc
      541 tggcagcaga tgcagctctt tcagagggct acgtaatctc tgataaaaat caatctgcgg
      601 ttggcgatgc accacatagt catcacctga tatcagaagt tgcgtgggga gcgtaatcgc
      661 cgccgcatcg ctaacaatac gttcagacgt tttatagaga tcgagcaaga tattgacggc
      721 aatcgcccgt gtgatcagcg gatcattatt gaaactcgcc acccgttgcc gatcgtgggt
      781 caaatagcgc cctttcacat aggaattaat aaaaaataga ccacgcagac gatgccataa
      841 cgccagcgca ggacgtgcca gcggcacata caatttaacc ttaaaggccg gagaagccag
      901 caccagccca cgaattgcag gtgcataatc atgaacccat gtggcaacaa gcactgcgcc
      961 gacgctttgc gcgatcacta ccacctcttc cagtccgacc tggctgtcgc tggcagcaaa
     1021 acggacaaat tcatcgacat cctgcactga gcgcataaga gacgggctat aaccacgcgg
     1081 ccccgaattt tttccatgcc cccgggcatc ccaggcataa aaagcagtat caggcatcgc
     1141 cagttcatca acgagatgtt gtagacgacc agaatgttca tgcccgcgat gaaataagac
     1201 gatgaccttt ttcgcccctg gctgtaaagt gggccagtgc cgataataca atgctgtatt
     1261 atcactggtg gtaaaaaaat gttccctagg gatgcgtgaa ttttccattt tctctatttt
     1321 ccttcagtaa cagcagtagc gaataaaata aaaaaatacc ggagcggtga aaattaatga
     1381 gtcaatcctg tcaaggagtc cgccatgtcc tagtaatagt tttccactat ctttaacacc
     1441 aatatctcgt ttgatggctg acatcactac atcgccgcaa aaaccactaa taccaattaa
     1501 caaacccgct aataatgcct gtaatgtatt tagcggtgtc agcaacggtc caataatcag
     1561 tgacgcaatc atggtggtga ttacgccccc cagcagacct tccagcgttt ttcccgggct
     1621 gactttgggg accacttttc ttctgccgca ggatttcccc cataaatact gtgcaatatc
     1681 gtttgactcg gttaacgcca aaagaaataa caccagtaac gccccgccct ggatatttat
     1741 ggttggcaac attaatagcc aggcggcatg actcaaggca aaaacggtca ttatccatcc
     1801 ccaaaaaatg gcgctcacgg tatgctgaaa accggagggg tcacccacaa aaactcgcca
     1861 ggtggctaat atcagaaacc cagtcaaagg tataaataac agaaacagct caaagcaatt
     1921 aaaaccgata agcaaatagt taagaggaat aacccaatat aaccaacgcg aaaaatgtac
     1981 ggatataagc gtacaatatt ctttcaatgc cagaaaactt atcagcgtaa aaaacgtcaa
     2041 cgtcatccag cgtgggcctg aaatcaccag tgaaaaacaa atgacgatgc cccaccaggt
     2101 acgaatacgt aatgtaactt catccccttt tctctcaggt aaccgccata gcagaaaacg
     2161 gtttatcagc ttgctaaaat taacaacgcg aaaagtgttg ccagagattt ttccagcatt
     2221 atctttcagc gctcataaga agcacactcc gacaacgatt aatcgcagta cagataagca
     2281 gaattgaagc aatgctccat aaaagattat tccattgcat ccactgtgga taaatggcaa
     2341 cggccagacc ccacagacca aatattaacg cgcgatcact tttgccaaat ggaccggcat
     2401 aactgcgaac accgttaatc gtctgcgcga gtaaaccgca aaactcggtc aatatggtgc
     2461 aaaataacat gagtatcacg agtgatgcgt tactttccgg taaaaataaa aacggtaaat
     2521 agagcgcaat atcggaaata acatcgccag tctcattcaa aatcgccccc agacgtgttt
     2581 gctgattgca ctcacgcgcc aacatgccat ccagcgcatt gagcgccata cggataaaaa
     2641 gcacgatggg caatagcaaa aagaggatgg gttgtgccac caacaccaac agcaatccgg
     2701 taaaaagaga aagcgccagt gccgtaagag tgatgtgatt cgctgtaacg tggtgcttat
     2761 aaagccaaaa catcgtcggc cttaacagcg actgaaagag cggttttatc tggtagagcg
     2821 tcattatcaa tccttgataa ttcggtttgc gctatggtgc atcaattccc ctgacggaga
     2881 tatcgcaaca cgccgcttca ttaaaaattc agaatatatc aaggtattgg gggctcactg
     2941 tcaggaatga ttatccccaa taaatcaagt agttaaaaat tattacgctg ttattcaata
     3001 caactcatca agtttcctca tcgcttgttc ccgtattgcg cgcgtcttac cgttaagcgc
     3061 cagctggcgg actaaatcca gttcagggtt aacaagctct aacgccagca aaggatcacg
     3121 tttcagcctc gccttcaata catcaactgg aaagcgggga ttattcatcg ccacaatcca
     3181 ccactcggga tcaatttctg ccgccagtgc cgcagagggg gtgtggcggt tgctaagtgc
     3241 gccgaacttg aaaaatgtga gattgctttt cgccagtgta ctccaccata tacgatccgg
     3301 ttgttttgcc agtacatcca gttgtgctaa cgcatcgggg tcgagtgcat aataaacacg
     3361 taatgcttca tcaacctgcg cacgggagct attttcctgc ctcagaaaaa acttcgccca
     3421 aatatcattg cgtggcgtgt tttgctgtaa ttccttgaga acaacagcgg gtaaattatc
     3481 ccaactaact aatgcactct ctaaaaagtc ccagtcttcc aaacgtatca gggcggcggc
     3541 atttaatagc atctggtcat caacttcgcc gtggctaatc agctcagaca aaatcatatc
     3601 agcgctggtt tcatatgcat ctgaatctgc tttcggatta tttttccgat gcgataaagc
     3661 acgttgccaa attttttgtt tcatcgacgt gctcagatac tgatttttcg ccagattact
     3721 ccacccctca ggatcgttga aacgtgtcaa taaccgttgt aaagcagcgt cagagtgaat
     3781 tacctgcata tcccgacagc aaaagtaggc aggggatttt ttctccatcc ttttaacaaa
     3841 attaaactgt aagttatctg gcaatgctaa aaataacaca cgtattagat catcgtcatt
     3901 ctcgaatttt tggtataaat aggctgcaac tttgtcactc tcttgtggcg tcatccgcaa
     3961 taattgacca tggtgtttca tctctgccag cttgaacgct aagcttgtga caacattgtt
     4021 tgatgaatcg ttcgccaact gcattgcctg attgtaagtg agttgtcctc cagcaccgac
     4081 ctgacgaata gaggaatcat cactgcggat atagccatca ataatggatt cgggcaataa
     4141 ccacgcatct ttataaagct cctgaatgat catatcccaa tctcttttcg ccagagtcat
     4201 gacgatatcc agagggagat tttcacgtag cgcaagattc aacggaccga caccatcagg
     4261 tagacgactc attcgcgtca ttatttcgac agagagcggt tcacggtcga gcaaccccag
     4321 aacaacaggc tggtaaccca attgctgatc ggtcaactcc agaatctcat tgtcaggcat
     4381 atatctcgcc atactttcaa tcgtttgttc atcattgcta ttaataagtt gtttgcgtag
     4441 gttagctgaa attcctttaa gccttgggag agttcgcatt atggcaagat caaatgtcgc
     4501 aatatctcgt ccttcaaatg cagcaacgtt gccttcatcc agcatttgca attcctgcac
     4561 taactcagga gaaggcttag ggcgaaacag cgcttctttt ttcatctgat tacagacatt
     4621 ccctgccatg cgaatattgc ttcgcttaaa aatttctttc agagcatctt catcatttat
     4681 tagaccccaa taatatgcat caggatttat tatcatcagc gcttttttga cattttcagt
     4741 gtcatttttg tcactcgcac ggaccatgaa ttcactattt gtcgaagacg caacaacagg
     4801 tgcaattatg tcattgataa ataccccacg cgcattaata ctccccttaa tcgtag
//
LOCUS       DAJTNP010000189         4777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTNP010000189 DAJTNP010000000
VERSION     DAJTNP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(6..335)
                     /locus_tag="OO160_003993"
     CDS             complement(6..335)
                     /locus_tag="OO160_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5525897.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(340..525)
                     /locus_tag="OO160_003994"
     CDS             complement(340..525)
                     /locus_tag="OO160_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5525898.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(522..3161)
                     /locus_tag="OO160_003995"
     CDS             complement(522..3161)
                     /locus_tag="OO160_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5525899.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3369..4358
                     /gene="ldhA"
                     /locus_tag="OO160_003996"
     CDS             3369..4358
                     /gene="ldhA"
                     /locus_tag="OO160_003996"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5525900.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4469..>4777
                     /gene="hslJ"
                     /locus_tag="OO160_003997"
     CDS             4469..>4777
                     /gene="hslJ"
                     /locus_tag="OO160_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5525901.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1209 a         1248 c         1113 g         1207 t
ORIGIN      
        1 tgctattaaa actttcgcac ccatttaccg tttatccctt gtacatattc ccccggtttg
       61 gcccgagcta cgagcttttg cccggcgagt ttcgcgatat catctacaga cacattattt
      121 tgctttgccc gttgttggta actcgcttta cgttcggcat taatttcggt tactaatttc
      181 tctgtttcag catccgtttt cagcgcaacc agataaccat aaaatgtttc acctacccgc
      241 ccctgagttc tggcttcatc cagagtcaat gccattacat tgctgcttac cagcccaaca
      301 agaaacgcac aaagaagtaa tgttttcttc atcatcgcct cagaaaagat cgctacgagt
      361 ttcaagcagc tcttcgacat ctttgtctgc cttgatgatg atctcatgct caattttaac
      421 gttcatattg atagtgatcg gttccttagg tgcagcgact tcaatgcgag gcgtacagcc
      481 aaccagcata aaagatgacg tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac
      541 tcttttcctt gcggacagtc atttcccggc agacgtgcgt tctgctccaa ccatgcctgg
      601 agattatcgc caaagcgtaa actgcgccac agcgtaaaaa tattctcttc atgatggtaa
      661 ttaaggttta ccgtaccgct tttaccatca acgcgactgg tacctgtaat gttggcctgc
      721 atggttaata aaccgagatt atctaagtta atttttgtcg atgaccggct aatttccata
      781 tagcgcaacc agttaattgc tgaacccgca gtcatattgt ctttcaccac cgcatctgcg
      841 gtatctttat ccagccgcaa ggtcattagc ccgctatttg ccagccaacc atctttcact
      901 atccattttt cgttgtttag ccataacggc aatgcaccac taaatgcccc ggacatggcg
      961 aattgtttcg gattgacggc gctaaccagt tcgctggatg agagattatt cagccgcaac
     1021 agcgccgggt catgttgcgg catacgtaat tgtttcatca gcacgttacc gcctaacaca
     1081 tcgacgctaa catcagtcaa cagcaagggt tcttcttcag tccacggata acgcccttgc
     1141 aaatcagccg taatattttt cgctgtcacc agattaatca cttcggcaat tcgcaacgta
     1201 acggggccgc gagtccccag atgccaggct ccatcggcaa aacggaaagg caggacaaaa
     1261 tcgacaccgt taacctgatt atctggcatc caggcactac cgcctttcaa cacgccgtgc
     1321 cctcccgcgc ggaatccttg ttcaggcgca gcagaaaatg caacctgagc atacagttca
     1381 ccatcgcgta agttcatctt ccagtcgggt ggcaccagcg gctggaatac agtcagtgat
     1441 tgttttggcc accaggcgtt accgcgcaga cgaataccgt cccagcgacc atttacccga
     1501 accgggccaa tctcaccagc atgtaaatcg cctttaaaga ggaaataggt cggattgcgc
     1561 ccatcgacgc taaattttaa ggttgatggc ggtaacacac tgccgccagt gaacagcgtt
     1621 tgcccggcgt ccagtgacag cgcgccgctg aaggagggat gctgtgcgtc acgtacccag
     1681 acgacgggct tgtcgagaat taatcgcggc ttttctaccg tcatcgtacc gtattgtaac
     1741 tggtcgaaac cggtggagag atcggtcagc gtaatcgtgc tgtcatgcca ctcacctttt
     1801 cctgcgacat cccaggtggc attcatcggt gtaaaactcc cttttcccca gtagcgccac
     1861 tgccagcggc cagcgtcagg gagaaaatca ttcgccagcc catccatatg cagcacgaaa
     1921 tcgcccagtt cattttcatg cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt
     1981 tgggtgactt ttacacccgc taaaggccag cggatttcat cgatgtccag cgaatcgatg
     2041 actcttccct ttgaacgaag taacgcgcct ggctcaaagg tcagcgttgg gtcagacaga
     2101 cttccactta gctgcgcagg taaacgggca tataaaatga ggtccgcctg tttcgcttca
     2161 ccggtcagct gcagaggcag ttgactgtta tccatgctta attttcctgg gccaaaatta
     2221 agcaccgcgt tgcccttacc cgcttgcccc tgggtcagca cattcagtcg tccgctgacc
     2281 agagcgttct caagccctgc ctgccagttg tcgactttga cacccagtcg gccactcaaa
     2341 ggaaaaccag catacagcca gctccagcga ccatcgctta cggtcaattg ttgacgagta
     2401 atttgccacg gcaaatcgag caacggatcg ccgttatccc gtgccagcac gatcaattgc
     2461 ccgctatttt cctgccagtc cagctcggca tccaccagtg acggttcctg cggcaagttt
     2521 aacgtcgcag tagcatgccc acttacagga agtccatccg gcacgagcgg catagtaaat
     2581 tcccccacca gtttaaccgg cggctgattt tcaaacgcga cgacatccag ttcgctgact
     2641 gtaagttgtt gccctttcag ctggccttga aatttaactt tttcgccctg ataacgcagt
     2701 tgctggatat cagaggttaa tgcgagagag agttttccct gccattcctg ccagggagaa
     2761 aaaatcagtt tatcgatatt gatccaggtg ttaggcagca tggactgcca ctgcgcgagg
     2821 gtttttggag cagctggcga ttgctccgtc tgcggcaatt tcgccagaca agcagaatca
     2881 agttctaccg tgccgacgtt caataaccag cggctgggat gtgaaaggct ggcgttggtg
     2941 atatgcgcaa gctgacaatc tcccaccaga taacggagat cgggaatgat taaaccttta
     3001 cgcgtaatgc gtgggctttc atctaatgca atacgtgtcc cgagcggtag ccagatgtcc
     3061 gccagcgtgg gaacccacag cccgagcgtc atcagcagcg tcaacggcac aagaataatc
     3121 agtaataaca gcgcgagaac ggctttatat ttacccagca tgggtagtta atatcctgat
     3181 ttagcgaaaa attaagcatt caatacgggt attgtggcat gtttaaccgt tcagttgaag
     3241 gttgcgccta cactaagcat agttgttgat gaatttttca atatcgccat ggctttcaat
     3301 tatatttgaa attttgtaaa atatttttag tagcttaaat gtgattcaac atcactggag
     3361 aaagtcttat gaaactcgcc gtttatagca caaaacagta cgacaagaag tacctgcaac
     3421 aggtgaacga gtcctttggc tttgagctgg aattttttga ctttctgctg acggaaaaaa
     3481 ccgctaaaac tgccaatggc tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc
     3541 acccggtgct ggaagagctg aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg
     3601 gtttcaataa cgtcgacctt gatgcggcaa aagaactggg gctgaaagta gtccgtgttc
     3661 cagcctatga tccagaggcc gttgctgaac acgccatcgg tatgatgatg acgctgaacc
     3721 gccgtattca ccgcgcgtat cagcgtaccc gtgacgctaa cttctctctg gaaggtctga
     3781 ccggctttac tatgtatggc aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg
     3841 cgatgctgcg cattctgaaa ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa
     3901 gtgcagcggc gctggaactc ggtgtggagt atgtcgatct gccaaccctg ttctctgaat
     3961 cagacgttat ctctctgcac tgcccgctga caccggaaaa ctaccatctg ttgaacgaag
     4021 ccgccttcga tcagatgaaa aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga
     4081 ttgattctca ggcggcaatt gaagcgctga aaaatcagaa aattggttcg ttgggtatgg
     4141 acgtctatga gaacgaacgc gatctgttct ttgaagataa atccaacgac gtgatccagg
     4201 atgacgtatt ccgtcgcctg tctgcctgcc acaacgtgct gtttaccggg caccaggcat
     4261 tcctgacagc agaagctctg accagtattt ctcagactac gctgcaaaac ttaagcaatc
     4321 tggaaaaagg tgaaacctgc ccgaacgaac tggtttaatc ttgcagctcc cctgcaatcc
     4381 aggggagctg attcagataa tccccaatga cctttcattc tctattctta aaatagtcct
     4441 gagtcagaaa ctgtaattga gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag
     4501 cctgctgatg gcgggatgtg taagtaatga caaaattgct gttacgccag aacagctaca
     4561 gcatcatcgc tttgtgctgg aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc
     4621 gccagaaatc agctttggtg aaaaaatgat gatttccggc agcatgtgta accgctttag
     4681 cggtgaaggc aaactgtcta atggtgaact gacagccaaa gggctggcaa tgacccgtat
     4741 gatgtgcgct aacccgcagc ttaatgaact cgataac
//
LOCUS       DAJTNP010000190         4670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTNP010000190 DAJTNP010000000
VERSION     DAJTNP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(106..690)
                     /gene="kptA"
                     /locus_tag="OO160_003998"
     CDS             complement(106..690)
                     /gene="kptA"
                     /locus_tag="OO160_003998"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5525902.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            940..1623
                     /locus_tag="OO160_003999"
     CDS             940..1623
                     /locus_tag="OO160_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5525903.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1620..2081
                     /locus_tag="OO160_004000"
     CDS             1620..2081
                     /locus_tag="OO160_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5525904.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2094..3266
                     /gene="iadA"
                     /locus_tag="OO160_004001"
     CDS             2094..3266
                     /gene="iadA"
                     /locus_tag="OO160_004001"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5525905.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3331..4242
                     /gene="hypT"
                     /locus_tag="OO160_004002"
                     /gene_synonym="qseD"
     CDS             3331..4242
                     /gene="hypT"
                     /locus_tag="OO160_004002"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5525906.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4235..4627)
                     /gene="iraD"
                     /locus_tag="OO160_004003"
     CDS             complement(4235..4627)
                     /gene="iraD"
                     /locus_tag="OO160_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5525907.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
BASE COUNT         1080 a         1234 c         1192 g         1164 t
ORIGIN      
        1 aaggataccc ttttacgagg gtatcccttc agactgataa caaacgctac attgccggat
       61 gtgctgcgct taccaggcct atataacccc tgcaatatat tgaatttatt gtttttgtac
      121 cccagtcggc ataaacgacc actcaagaaa ctcaacagca accgttgatg tcagccagac
      181 cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg ctttgacggt
      241 taagacaacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt catcagcaga
      301 gagatgcacg taatgacgat cccctgcaat cagcccctgt ttttttatct cgtcaaggaa
      361 acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa aggaaatagc
      421 gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac tggaataact
      481 aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc aatgcacggg ttaatcgctt
      541 gcccgctttt tgggcacaaa gtatgagctt attgatatcc gcccatcctt cacggtccag
      601 tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact tgctcgtatc
      661 cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt ttctgttttt
      721 atttactaat gtcattgtca ctgggtttat gaattttctg caactcaatc ctgcgcagaa
      781 ccttttccac acagcgtaat ttttccccta tgcaaaaaac gcatagccag aaacatactt
      841 gcattttccc catacccaca tggctgataa ggtttttcag ttccaggaat tttcctggct
      901 caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca aggggatgct
      961 gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc tttttttctc
     1021 accataatct tttttcccgg tgttttttcc ggaacagaca gctggtggcg cgtttttgat
     1081 ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaatggagc caccacctca
     1141 tttcgcgggg tgggtggcgc gggggctaaa gatggctttc tctttgcgct ggagctggca
     1201 ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg cggattacgc
     1261 gccgcgcagc agttaatgac acctgtgcta aaaccgttgt tgggcattcc agggatctgc
     1321 tcgctggcac tgatcgctaa cctgcaaaac acggatgccg ccgccggtat gaccaaagag
     1381 ctggcgcagg aaggtgaaat taccgaacgc gataaagtga ttttcgccgc atatcaaacc
     1441 agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt tgcctttctg
     1501 ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt cggagccaac
     1561 attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg agcgcaagca
     1621 tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg cgccgtggct
     1681 ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc atcattcagg
     1741 cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc gtaatggcgc
     1801 tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg agtatgggcg
     1861 gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc attaaccggc cacgatgtca
     1921 ccgttttact gcccgcaatg tatttaatgg gcaacccggt gcaaaatgtc ggtcgctgcc
     1981 tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc tgcgtgatta
     2041 acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt accatgattg
     2101 attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg ccggaagatc
     2161 ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc agcaatatcc
     2221 cttctgacat tgtaccgaac tgcacggttg tcgatctcag cgggcagatc ctctgcccag
     2281 gttttattga tcaacacgtc catttgattg gcggtggcgg cgaagcaggt cccacgacgc
     2341 gcacgccgga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca gtggttggtc
     2401 tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag acccgtgcgc
     2461 tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc ccttcccgca
     2521 ctattacggg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt ggcgtgaaat
     2581 gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct aatatggcgg
     2641 cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg ttccacatgg
     2701 gcgacagtaa aaaggcgtta aagcctattt atgacctgct ggaaaactgc gatgtgccga
     2761 tcagcaagct gctgccgacc cacgttaacc gcaacgtacc gttgtttgag caggcgctgg
     2821 agttcgcgcg caaaggcggc accatcgata tcaccagcag cattgacgaa ccggtcgccc
     2881 ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc gtcaccctca
     2941 gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta acccatatcg
     3001 gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa gactatgatt
     3061 tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc cttaacatga
     3121 gcgggaaagg cgaaattctg ccaggcaatg acgcagactt actggtcatg acgccagaat
     3181 tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc aaagcctgcg
     3241 tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg ccctaatgga cgtagaatgc
     3301 ccccaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcggt tttgcataat
     3361 attgaaacca aatggcttta tgattttctg actctggaaa aatgccgcaa tttttcccag
     3421 gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg tgcactggaa
     3481 caggcgattg gcgttgaatt gtttaaccgc caggtgacgc cactgcaact ctcagaacaa
     3541 gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagaaag caaccttgca
     3601 gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca agatagccgc tgcacactct
     3661 ctttccctcg gtctgttacc gtccattatc agccagatgc cgccgctctt tacctgggca
     3721 attgaagcca ttgatgtcga tgaagcggtc gataaactgc gtgaagggca aagtgactgt
     3781 attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat tcgcttattt
     3841 gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact ttttaacctc
     3901 gcgcagccac actttccgtt actgaattac agccgcaact cctacatggt gcgattgatt
     3961 aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt ctcttcgatg
     4021 agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct gccggagtat
     4081 gccatccaac aagaaattcg cagcgggcag ctcgttgtgc tgaatcggga cgaacaggtg
     4141 atcccgattc aggcctacgc ataccggatg aatacccgca tgaatccagt tgccgaacgc
     4201 ttctggcgtg aactgcgcga gctggagatt gtgcttagct gacattctca agcgtcgcac
     4261 tgcgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg tcgtggtaca
     4321 gcattatctc aaggctgatc gccagagtgt ggcagtacgc ttctttcaaa ttaacctgca
     4381 acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg caataccagc
     4441 tctggagatg tttgtcgctc gggttatgca cttgccactc gggcatgcct gaaacaagta
     4501 atgacggtaa tgccgaatga ggagaattaa aaagagtgag gagatcgctg cataccgatt
     4561 tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga agtgattggc
     4621 gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg
//
LOCUS       DAJTNP010000191         4592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTNP010000191 DAJTNP010000000
VERSION     DAJTNP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(68..856)
                     /gene="phoH"
                     /locus_tag="OO160_004004"
     CDS             complement(68..856)
                     /gene="phoH"
                     /locus_tag="OO160_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5525908.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1463..2728)
                     /gene="efeB"
                     /locus_tag="OO160_004005"
     CDS             complement(1463..2728)
                     /gene="efeB"
                     /locus_tag="OO160_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5525909.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2734..3861)
                     /gene="efeO"
                     /locus_tag="OO160_004006"
     CDS             complement(2734..3861)
                     /gene="efeO"
                     /locus_tag="OO160_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5525910.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3919..>4592)
                     /locus_tag="OO160_004007"
     CDS             complement(3919..>4592)
                     /locus_tag="OO160_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5525911.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1075 a         1295 c         1108 g         1114 t
ORIGIN      
        1 gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat
       61 aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt acgcaatcct
      121 cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
      181 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
      241 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
      301 gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata taggcgaacg
      361 gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
      421 agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
      481 caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
      541 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc
      601 cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc tgcagatagt
      661 gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg cgggccatac
      721 caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
      781 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
      841 ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
      901 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
      961 ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta acacgagtaa
     1021 agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga aatctccgcg
     1081 agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca tccgcgatct
     1141 ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag tacaacagaa
     1201 ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca tttcggtgac
     1261 attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc tgtgtgcttt
     1321 ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg gcgcgaacgc
     1381 cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg ataaacctgc
     1441 gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga taatcgttcg
     1501 cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta acgtattcct
     1561 ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc agatcgtgtt
     1621 ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc cccaacgaat
     1681 aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg ggattcgcca
     1741 gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg taatcaggca
     1801 catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg ccaaaaatcg
     1861 tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga atcaagcgta
     1921 ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc gttacccaca
     1981 ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg tctttgaaac
     2041 ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg gaaataaacc
     2101 cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata tcgcgcagcg
     2161 catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca ccatgacata
     2221 acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt tttggcatct
     2281 gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac gtgatggtga
     2341 gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc aggcgcggat
     2401 ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga gtcaacaagc
     2461 gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc accagcatca
     2521 ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac ggctgtttct
     2581 cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt gcatgagcga
     2641 ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt gacggttcat
     2701 tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca caccgcgaag
     2761 ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc ggtcggcatc
     2821 ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc
     2881 gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt tttgcagttg
     2941 tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag
     3001 atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca cttcctcaat
     3061 cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt
     3121 ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc ctttggtggt
     3181 gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg ggtcggcggc
     3241 tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc
     3301 agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt
     3361 ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt
     3421 ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc
     3481 atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt
     3541 agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg
     3601 gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc
     3661 ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc cggcgttaac
     3721 cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct gcggcacatc
     3781 agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt
     3841 acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa
     3901 gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag
     3961 agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct
     4021 cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
     4081 gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
     4141 aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa acaggctggt
     4201 ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca ggaagccgag
     4261 caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg
     4321 ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa
     4381 gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc tatcgactgc
     4441 ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa ccatccaggt
     4501 aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct ctttttgcgg
     4561 aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTNP010000192         4553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTNP010000192 DAJTNP010000000
VERSION     DAJTNP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..1225
                     /locus_tag="OO160_004008"
     CDS             <1..1225
                     /locus_tag="OO160_004008"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5525912.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1225..1959
                     /locus_tag="OO160_004009"
     CDS             1225..1959
                     /locus_tag="OO160_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5525913.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2427..3188
                     /locus_tag="OO160_004010"
     CDS             2427..3188
                     /locus_tag="OO160_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5525914.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3232..4311
                     /locus_tag="OO160_004011"
     CDS             3232..4311
                     /locus_tag="OO160_004011"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5525915.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4308..>4553
                     /locus_tag="OO160_004012"
     CDS             4308..>4553
                     /locus_tag="OO160_004012"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5525916.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQL"
BASE COUNT         1247 a          865 c         1121 g         1320 t
ORIGIN      
        1 ctttccgtat ggcattttcc agcagcgaaa tgttcttgcg agctggctgg caacaggttg
       61 cggcgtagcg ttgtatctgg ctttaacggc gcgtactcgt tctctgacgt tgatatgttt
      121 gttgtcgtta taccctctgt ctacagcttt agtccttaca caaagccgtg tcggccttct
      181 tggtttattt tgcgtcatcg tgctggccgc tctcgcggac agaccccgct ggcgcggacg
      241 tccccttagc ggtctgttgc gcttgatctt cgtcgcctcc ttgctgtgct ggtgtgtcgg
      301 tatctcaaac tatgccatgc caggggagac aacggctgac tttacgcatt catcatcgaa
      361 tgaccagcgt atacgtattc tgaaaggtgc cataagtctt attcaacagc gccccttaac
      421 cggttggggg ttaggcagct ttgaagcgac cttcccgcaa gccttagagg atgtgggcgt
      481 gacaaacata gagagtgata cggctacgca tccccataac gaactgctgt tcgtaacggc
      541 tgaaggtggc atcgtcgcct tgtttggttt tttgctgcta tcaggcgttt ggctgtggcc
      601 gtgtatatgc cgcctgaaaa tgacaaacaa taacgcgcac catcaatgtg aaaagtggtt
      661 tattgcctta atggggttgc ctttagtcat tcatatgatg accgaatatc ctctctatac
      721 gtctgttccc catttgatgc tgttgctgct ccttttccgc atcggaatgc cggaaaacgt
      781 gatgaatcag gtacgtatac ctaaaacagt ccggttgggt attctgcctg tcattgtgct
      841 cattatgacc gccgcgctgg tcatcctggt agcgggattt gatgcacagc gggaactgac
      901 cagagctgaa gctgatatga acgaaggatt tatccctcgc cttccggaac atagctggaa
      961 atacctgacg caggcagaac gtcttgagcg cgatcagcat ctgcttattg cgaataaacc
     1021 ggggttcgtt cgcgatacgg aaagcatgtc tcttttcact gtgtggggaa ataaatggtt
     1081 ggcaatccac aatgatgcag acgtaagcgc tgcaatgata ttcattgcgc acaggcgcgg
     1141 tgattatctg acagctgaaa aaataagaaa acgcgcagct cgggtatttg taaatgacga
     1201 acgttttact cgcgggggaa actaatggtt aacgatctga cgtataagaa attagaaagc
     1261 acaaacatca actgtaatat tctctcgcca gaaaatacca catggacaaa tgtcacggtg
     1321 attggcctgg atctttttgc catttgcggc gtgtgcagtt tgctcaagcg tgttggggga
     1381 gtgaaacttc aggttttcac ttccttaccc actgatatga atgttctgac tgacagtagc
     1441 atcgtgatat gggtaaaaat gcgtcacgat ggtttgcctg aattagccgg acatattgtg
     1501 aaggtttgtc gtcgataccg tctgatgcgg caactcgtta ttagcgatgc tattcctgaa
     1561 ggaatgacga atgatatcgg gcctctttcc cgagtatggc ttactcatgg gagtgcaaaa
     1621 gtggatattt tattatcttc attacggaag gtgttaaatg ctcattgttt gcctggtcct
     1681 ttgtttataa aacgcctggg aagagtgcaa tggagggttc ttttactccg cgcgaagggg
     1741 attagcacac gtaaaattgc agaaatctgt ggtattggcc tcaagaccgt tagcgcccat
     1801 gagtccgcaa ttcgagaacg attaggcacc aggaataaaa gtgagtacgt ctggttagta
     1861 cgtaatgctc aacttataca acaggcgata cccgcgttgg gaagagatgc gtcaaggtat
     1921 gaaagatgta acaatatgcg aacaaagcaa aaagtataat tgatgcgtca tagataactc
     1981 tctttttcga tgacgaaatc gctgagcatg cgtggtgagg ttagtaatag atgggtgcta
     2041 tgttttgcca cgggtttgta gctgtgaaac ctggaaaaaa ataagaatta aaaatattta
     2101 aagagagtta ataaggttct ggttgtaatt gtcttggtca aaaaaaagat attgtcaatg
     2161 ttgtttttta aattttcagg ttttggttaa atatttgctt gcaaatatag gtgatttttt
     2221 tattgtaggc ttttgaaggc tattataact gtgcgctaaa ttaatgcgtt gaataaaaga
     2281 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
     2341 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
     2401 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
     2461 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
     2521 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
     2581 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgcaga
     2641 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
     2701 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
     2761 aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa aagagtgatg
     2821 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
     2881 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
     2941 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
     3001 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
     3061 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
     3121 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttata tctcatttgc
     3181 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
     3241 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     3301 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     3361 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     3421 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     3481 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     3541 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     3601 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     3661 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatggtg
     3721 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     3781 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     3841 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     3901 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     3961 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     4021 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     4081 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     4141 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     4201 aatggcgagg agcagtattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     4261 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     4321 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     4381 gagggattca aaacgccact cattttaatg caatatctac gggtattggc atttctctgg
     4441 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     4501 ataaatatcg tattcgttat agtaaatcca ttagtgattt tggtactcaa ttg
//
LOCUS       DAJTNP010000193         4500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTNP010000193 DAJTNP010000000
VERSION     DAJTNP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..239
                     /locus_tag="OO160_004013"
     CDS             <1..239
                     /locus_tag="OO160_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR5525917.1"
                     /translation="DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYV
                     DRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKK"
     gene            325..1941
                     /gene="bglH"
                     /locus_tag="OO160_004014"
     CDS             325..1941
                     /gene="bglH"
                     /locus_tag="OO160_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5525918.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            1968..3143
                     /locus_tag="OO160_004015"
     CDS             1968..3143
                     /locus_tag="OO160_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5525919.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3145..3867
                     /locus_tag="OO160_004016"
     CDS             3145..3867
                     /locus_tag="OO160_004016"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5525920.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(3929..>4500)
                     /locus_tag="OO160_004017"
     CDS             complement(3929..>4500)
                     /locus_tag="OO160_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5525921.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1245 a         1007 c         1098 g         1150 t
ORIGIN      
        1 acgatcacct ggtacaggta aatgaagcga ttgccgatgg tgtggatatt atggggtaca
       61 ccagttgggg gccaattgat ttagtcagtg catctcattc acaaatgtct aagcgctacg
      121 gctttattta tgtggatcgt gatgataatg gcgaaggaag cctcacaaga acgcgtaaga
      181 aaagcttcgg atggtatgca gaagtgatca aaacgcgggg gctgtcatta aaaaaataac
      241 caataaagca ccttaattat cgtcgcattc agaacagtct ggatgcgata cgttaattct
      301 ttctttgcac cataaaggga tattatgttt agacaaaatc ttattacctc tgccatctta
      361 ttaatggcac cgttagcctt ttctgcacaa tcattggctg aatcattaac ggtggaacaa
      421 cgccttgagt tattagaaaa agcgttaaga gaaacgcaaa gcgaactcaa aaagtataaa
      481 gatgaagaga agaaaaaata tacgccagcg acggtgaatc gtagcgtaag tacgaatgat
      541 caagggtatg ccgccaatcc gttcccgacc agtagtgccg caaaacctga tgctgtactg
      601 gtcaaaaatg aagagaaaaa tgccagtgag acaggctcga tttattcttc catgactctg
      661 aaagatttca gtaagtttgt gaaagatgaa attggcttta gttacaacgg ctattaccgt
      721 tctggttggg ggaccgcctc tcatggttca cctaaatcat gggcgattgg ttctctgggc
      781 cgctttggta acgaatactc cggctggttt gatttgcagt taaaacaacg tgtctacaac
      841 gaaaacggca aacgggttga tgccgttgtg atgatagatg gtaacgttgg tcagcagtac
      901 tctaccggct ggtttggcga taatgccggt ggcgagaact ttatgcagtt ctccgatatg
      961 tacgttacca ccaaaggttt cctgcccttt gcgccagagg ctgatttctg ggtgggtaaa
     1021 cacggtgcgc cgaaaattga aatccagatg ctggactgga aaacgcagcg tactgatgct
     1081 gcagcgggtg taggtctgga aaactggaaa gtcggtccgg gtaaaattga tatcgcgctg
     1141 gttcgcgaag atgtcgatga ttacgatcgc agcctgcaaa acaaacagca gattaatacc
     1201 catactattg atttacgcta taaagatatc ccgttatggg ataaagcgac cttaatggtg
     1261 agtggtcgtt atgtcacggc aaacgaaagc gcatcggaaa aagataatca ggataataac
     1321 gggtattatg actggaaaga tacctggatg ttcggcacat ctttaacgca gaaatttgat
     1381 aaaggtggct tcaacgaatt ctccttcctg gttgcgaata actctatcgc caggaacttt
     1441 ggccgttatg ctggcgcaag tccatttacc acctttaatg gtcgttatta tggtgatcac
     1501 accggcggaa cagcagttcg tctgacctcg cagggcgaag cctatatcgg cgatcatttt
     1561 attgtggcta acgcgattgt ttactccttc ggtaacaata tttatagcta cgaaacaggc
     1621 gcacactctg atttcgaatc tattcgtgcg gttgttcgcc cggcctatat ttgggaccaa
     1681 tataaccaga caggtgttga actgggctat ttcacccagc aaaacaaaga tgcgaatagt
     1741 aataaattta atgagtctgg ttataaaacc acgctcttcc atacctttaa agtcaatacc
     1801 agtatgttga cctcgcgtct ggaaattcgt ttctacgcca cgtatatcaa agccctggaa
     1861 aacgaactgg atggcttcac cttcgaagac aataaagacg cccagtttgc tgtcggtgcc
     1921 caggctgaaa tctggtggta atggaactta atgaaaggaa tattacgatg aatataaaaa
     1981 ttgctgcttt aacgctggct attgccagcg gtatttctgc tcagtgggcc atagcagcgg
     2041 atatgccagc cagcccggca cccactatcc cggtgaaaca gtatgtgact caggtcaatg
     2101 ccgataacag cgtgaccttt cgctactttg cccctggggc aaaaaatgtc tctgtagtgg
     2161 tgggtgttcc ggttccggac aatattcacc cgatgaccaa agacgaagca ggagtctggt
     2221 cgtggcgcac acctatcctg aaaggcaacc tgtacgagta ttttttcaat gttgatggtg
     2281 tacgcagcat tgatacaggc accgcaatga ctaagcctca gcgccaggtt aactccagta
     2341 tgattctggt gccaggcagt tatctggata cgcgttctgt tgcgcatggt gatttgatcg
     2401 ccataactta ccactccaac gcattgcaat ctgaacgtca gatgtatgtc tggaccccgc
     2461 caggatacac cggcatgggc gagcctttgc cagtgctcta tttctatcac ggttttggtg
     2521 ataccggacg ttccgctatc gatcaggggc gtatcccgca aatcatggat aacctgctgg
     2581 ctgaagggaa aattaaaccg atgctggtgg tgatcccgga taccgaaacc gatgcgaagg
     2641 gcattattcc cgaagatttc gtgcctcagg aaagacgtaa agtcttttat ccgctgaatg
     2701 ctaaagcggc agatcgcgaa ctgatgaacg atattatccc gctgattagc aagcgtttta
     2761 gtgtccgtaa agatgccgat ggtcgcgcgc tggcagggct ttcacaaggc gggtaccagg
     2821 cgctggtttc cggaatgaat catctggaaa gctttggctg gctggccaca ttcagtggtg
     2881 ttaccacgac aaccgtaccg gatgaaggtg tcgcggcccg gctgaacgat ccggcagcta
     2941 ttaaccagca actacgtaat tttactgtgg tcgttggtga taaagatgtc gtaaccggca
     3001 aggatatcgc cgggctgaaa actgagcttg agcagaaaaa aattaagttt gattaccagg
     3061 aatatcccgg cctgaaccat gaaatggatg tctggcggcc tgcctatgca gccttcgtac
     3121 agaaatttaa aataaggcat taagatgaaa ttaatcatta ccgaagatta ccaggaaatg
     3181 agccgtgtcg cggcacacca tctgcttggt tatatgtcga agacgcgtcg tgttaacctg
     3241 gcaattaccg ccggtagcac gcccaaaggc atgtatgaat acctcaccac cctggttaaa
     3301 ggtaagcctt ggtacgataa ctgctatttc tataatttcg atgaaattcc ctttcgcggc
     3361 aaagagggag aaggcgtaac gattaccaat ctgcgtaatc tgtttttcac ccctgcgggg
     3421 atcaaagaag agaatatcca gaagctcact attgataact accgcgagca tgatcagaaa
     3481 ctggcgcgtg aaggcggcct ggatttggtg gtgctgggat taggtgcaga tggtcatttt
     3541 tgtggcaatt tgccgaatac gacccatttc catgagcaaa ccgtggagtt cccgatccag
     3601 ggagagatgg ttgatattgt ggcgcatggt gagttgggcg gcgacttttc gttagtgcct
     3661 gacagctacg tgacgatggg gccgaaaagc atcatggcgg cgaaaaactt gctgataatt
     3721 gtcagtggcg caggtaaagc gcaggcactg aaaaatgtgc tgcaaggtcc ggttaccgaa
     3781 gatgttccag cttccgtgtt gcagctgcat ccttcgctga tggtaatcgc tgataaagcc
     3841 gctgccgcag aattagcgtt cggttaagct gaagaaataa ggccggatgt atgaattacg
     3901 ctccggcctg ttctcattat ttaaataact acgaaaaatc accccacagt ctcagcttgc
     3961 cgcagtggag acagcggaag agataaccct gacaatcacc acccttttgc aggcatttag
     4021 ctagatcaga atttctaata ccgaaattct cacaatcttc ttcaaggttg gcaaattcat
     4081 cgaggcgatc ttttatatca ttccagccca catagccgat aaaagcacag aaatcgccac
     4141 aatgcgcaag ccagaattcc tgttgccatc catgataacc tggcgtgcgt tcaaccaact
     4201 ctttcagcat ttcatcagga tatgtgttct taataccggc aaattcgtcc tcttcatcat
     4261 actcaaattc aacaccttct atgctggcat catcctgaaa actacctgca aacttttctg
     4321 cagcagaacc gtccgcaata cacaacggac agagatgttc aacttcatca acgcaataaa
     4381 agggacccga gtaataaact gacgtctgtt gttcacagca atcgcactct acggttttat
     4441 cctgttcaaa tgcgcctgtt tccagtggct tgggatgata tttgaatgta agcgccccga
//
LOCUS       DAJTNP010000194         4358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTNP010000194 DAJTNP010000000
VERSION     DAJTNP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..311
                     /locus_tag="OO160_004018"
     CDS             <1..311
                     /locus_tag="OO160_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5525922.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO160_004019"
     CDS             468..1217
                     /locus_tag="OO160_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5525923.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO160_004020"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO160_004020"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5525924.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO160_004021"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO160_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5525925.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO160_004022"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO160_004022"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5525926.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..>4358
                     /locus_tag="OO160_004023"
     CDS             4084..>4358
                     /locus_tag="OO160_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525927.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVV"
BASE COUNT         1170 a         1103 c         1075 g         1010 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgt
//
LOCUS       DAJTNP010000195         4317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTNP010000195 DAJTNP010000000
VERSION     DAJTNP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            4..327
                     /locus_tag="OO160_004024"
     CDS             4..327
                     /locus_tag="OO160_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5525928.1"
                     /translation="MATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLW
                     PVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAME
                     SRSLR"
     gene            524..1963
                     /gene="aldA"
                     /locus_tag="OO160_004025"
     CDS             524..1963
                     /gene="aldA"
                     /locus_tag="OO160_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5525929.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2005..3005)
                     /gene="gap"
                     /locus_tag="OO160_004026"
                     /pseudo
     CDS             complement(2005..3005)
                     /gene="gap"
                     /locus_tag="OO160_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3194..3724
                     /gene="cybB"
                     /locus_tag="OO160_004027"
     CDS             3194..3724
                     /gene="cybB"
                     /locus_tag="OO160_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5525930.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3969..4142
                     /locus_tag="OO160_004028"
     CDS             3969..4142
                     /locus_tag="OO160_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525931.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4214..>4317)
                     /gene="hokB"
                     /locus_tag="OO160_004029"
     CDS             complement(4214..>4317)
                     /gene="hokB"
                     /locus_tag="OO160_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5525932.1"
                     /translation="ILAFTLLTRQTLYELRFRDGDKEVAALMACTSR"
BASE COUNT         1097 a          988 c         1089 g         1143 t
ORIGIN      
        1 acgatggcaa cgttccgccg tatgaccggg gacaatcccg atgcaccacg ctggttaagt
       61 tatcccggat tcgttcctca gttaggaaat aacgcagaca gtgtaatctt tattaatcag
      121 ttacaaggat tatggccggt tgagcgttat ctctcactac tcactggcga gctgccgcgt
      181 ttacacgatg atagcgatgg ctacggtccc cgcgggcgag attttatcgt tcacgttgat
      241 tttccggcag aagtcatcca tgcatggcaa acgctgaaac atgatgcggt gctcatcgag
      301 gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa
      361 cgggcatgac tcctgacttt tatttctgcc ttttattcct tttacacttg tttttatgaa
      421 gccctccaca gacttgtcct ttcccgatgc cgtctctctg atgattgatg ttaattaaca
      481 atatattcac cgaaaacaaa catataaatc acaagagtcg cccatgtcag tacccgttca
      541 acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat ggattgatgt
      601 ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg ccgaggatgc
      661 ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat
      721 tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc cgcgaacgcg ccagtgaaat
      781 cagtgcgctg attgttgaag aagggggcaa gatccagcag ctagctgaag tcgaagtggc
      841 ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg agggcgagat
      901 tattcaaagc gatcgtccgg gagaaaatat tcttctgttt aaacgtgcgc ttggtgtgac
      961 taccggcatt ctgccgtgga actttccgtt cttcctcatt gcccgcaaaa tggctcccgc
     1021 tcttttgacc ggtaatacca tcgtcattaa acccagtgaa tttacgccaa acaatgcgat
     1081 tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc ggcgtgttta accttgtact
     1141 ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg caatggtcag
     1201 tatgacaggc agcgtctctg caggtgagaa gatcatggcg actgcggcga aaaacatcac
     1261 caaagtgtgt ctggaattgg gtggtaaagc accggctatc gtaatggacg atgccgatct
     1321 tgaactggca gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc aagtgtgtaa
     1381 ctgtgcagaa cgtgtttatg tacagaaagg catttatgat cagttcgtca atctgctggg
     1441 tgaagcgttg caggcggttc aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg
     1501 gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga
     1561 agaaggggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat attattatcc
     1621 gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg aaacctttgg
     1681 cccggtgctg ccagttgtcg catttgacac gctggaagat gctatctcaa tggctaatga
     1741 cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg cgatgaaagc
     1801 cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg aagctatgca
     1861 aggtttccac gccggatggc gtaaatccgg tattggcggc gcagatggta aacatggcct
     1921 gcatgaatat ctgcagaccc aggtggttta tttacagtct taatgagtga aagaggcgga
     1981 ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg tgcgaataag
     2041 ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca gttgtaaatc
     2101 gcccacggcg gtaatttccg tttgcgtggc atcaaacact gaaccgaaat ggctgccaat
     2161 gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat tggtggttgc
     2221 ttgtttaagt gcgttattca cctcttcggc agtcactttt ttccgagaat ggacaccagc
     2281 tcagtgaccg aacctgtttt caccggcacg cgttgcgcat gacctttcag tttgccgctc
     2341 agttccggga tcaccagacc aatggctttt gccgcccccg tagtgtgggg aatgatattt
     2401 tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac cagtgactgg
     2461 gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa actgtcatgc
     2521 aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg acgccacgga aacaatggtg
     2581 tcgttgccat ccagagtgtc gtcattaacg ttataaacga tggttttcat ttcaccggca
     2641 ggagcggaaa tcaacacctt cttcgcacca gcatcaagat gcgcctgcga tttatcggcg
     2701 gaggtataaa agccagtaca ttcgacaatg atttctgccc ctttcgcttt ccacggaata
     2761 tttttagcct ctttttcggc gtaaaccgcg atacttttcc catcaacgat aagtgaatct
     2821 tccgtaaaat caacgctcca ggggaacgga ccatagtttg aatcatgttt cagcagatag
     2881 gcgagaattt ttggggaagt aagatcatta atagcgacaa cgtctatgtt gcttttgact
     2941 tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa aaccgttaat accaacttta
     3001 ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc aggcttagac
     3061 caggattgtg ggatgggcaa tctccattct tacagtgaaa cgtaacgtac tgaaaacggg
     3121 tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg ttaacggaaa attatgttaa
     3181 agcaggaaat gttatggaaa ataaatactc aaggttacaa atcagcattc actggctggt
     3241 ctttttgctg gttatcgcag cgtattgcgc aatggagttt cgtggtttct tcccacgtag
     3301 cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc atctcaattc tcgtgctgat
     3361 ggtggttcgt cttttgttaa gactgaaata cccaaccccg ccgattatac ctaagccgaa
     3421 accgatgatg acgggactgg cgcatttggg acatttggtg atttatctcc tgtttattgc
     3481 gctgccagtg attggtttgg tgatgatgta taaccggggc aacccgtggt ttgcgtttgg
     3541 tttgacgatg ccttacgctt cagaggccaa tttcgaacgg gttgatagct taaagtcgtg
     3601 gcatgagacg ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg cggctgcact
     3661 ggcacaccac tatttctgga aggacaacac acttctacgc atgatgccgc gtaaacgttc
     3721 ctgaaggata tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat
     3781 acgggtctac atcggaagcg cctattatat ttatttgtat gacaaataaa accataatcc
     3841 ttgcccatac gtccatctgg cttattttta atcaattcac ccgatctttg atctcatcaa
     3901 cggtatcaaa ataaaccagc gtataacgtt caaatcgcac ataagattcg ataaaccatg
     3961 gaggttatat gaaaaaactg gcacttattt tgtttatggg aacgcttgtt tccttttatg
     4021 ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac tgtacggcag
     4081 gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca tgcactaact
     4141 gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag tcagatgcgc ggggaaaatc
     4201 cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc agcaacctcc ttatcaccgt
     4261 cccggaaccg cagttcgtag agcgtttgtc gggtcaggag tgtgaatgcc agaatcg
//
LOCUS       DAJTNP010000196         4211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTNP010000196 DAJTNP010000000
VERSION     DAJTNP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO160_004030"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO160_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5525933.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO160_004031"
     tRNA            complement(324..399)
                     /locus_tag="OO160_004031"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO160_004032"
     tRNA            complement(406..480)
                     /locus_tag="OO160_004032"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO160_004033"
     tRNA            complement(597..681)
                     /locus_tag="OO160_004033"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO160_004034"
     tRNA            complement(690..765)
                     /locus_tag="OO160_004034"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO160_004035"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO160_004035"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5525934.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO160_004036"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO160_004036"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5525935.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO160_004037"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO160_004037"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5525936.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1090 a         1047 c          936 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcaagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt c
//
LOCUS       DAJTNP010000197         4184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTNP010000197 DAJTNP010000000
VERSION     DAJTNP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..188)
                     /locus_tag="OO160_004038"
     CDS             complement(<1..188)
                     /locus_tag="OO160_004038"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5525937.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO160_004039"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO160_004039"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5525938.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="OO160_004040"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="OO160_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5525939.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="OO160_004041"
     CDS             3865..4146
                     /locus_tag="OO160_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5525940.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1046 c         1062 g         1060 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatcccta taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac aggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgggcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaa
//
LOCUS       DAJTNP010000198         4162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTNP010000198 DAJTNP010000000
VERSION     DAJTNP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            74..742
                     /locus_tag="OO160_004042"
     CDS             74..742
                     /locus_tag="OO160_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525941.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(985..1680)
                     /locus_tag="OO160_004043"
     CDS             complement(985..1680)
                     /locus_tag="OO160_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5525942.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1673..3100)
                     /locus_tag="OO160_004044"
     CDS             complement(1673..3100)
                     /locus_tag="OO160_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5525943.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3111..3830)
                     /locus_tag="OO160_004045"
     CDS             complement(3111..3830)
                     /locus_tag="OO160_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5525944.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1107 a         1080 c          862 g         1113 t
ORIGIN      
        1 ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac agattttatt
       61 ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg agattttatt
      121 tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta tggcaatggt
      181 gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc ttaaaataaa
      241 ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg cctgtcagac
      301 cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca gcccagatat
      361 caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac caactgctga
      421 agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aataccataa
      481 tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg agctttatac
      541 aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac
      601 tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt
      661 ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata
      721 ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg tataaagctg
      781 aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg
      841 taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata accatgacgg
      901 aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt
      961 ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca ccgctttcac
     1021 cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc tgggaccact
     1081 gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat gcaatttttc
     1141 tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc
     1201 cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga
     1261 ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg cctgcgagat
     1321 attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc
     1381 actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag
     1441 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca gctcacagcg
     1501 cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag
     1561 ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag
     1621 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat
     1681 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     1741 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     1801 cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact ttccacaatc
     1861 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     1921 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     1981 ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat
     2041 ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc
     2101 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc
     2161 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     2221 caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggccctgtca
     2281 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     2341 atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact gcaatatgcg
     2401 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     2461 cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt
     2521 cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc
     2581 ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca
     2641 caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga tcggtttcaa
     2701 tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     2761 atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg
     2821 tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     2881 agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc
     2941 gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccaa
     3001 tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga
     3061 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     3121 atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg taatcgccca
     3181 ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg gacttccatc
     3241 aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     3301 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     3361 acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc caggctacaa
     3421 gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac ccctaattta
     3481 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc
     3541 cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt gcaagagcca
     3601 gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     3661 ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca tccctgtttc
     3721 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     3781 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     3841 ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat caatcatcaa
     3901 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa
     3961 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     4021 cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt aaaaatggct
     4081 acatccctta tacctattca gagctactta ttcaggggtt attgagatat acactgatgg
     4141 tgtacaaaaa aatcaccgac aa
//
LOCUS       DAJTNP010000199         4158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTNP010000199 DAJTNP010000000
VERSION     DAJTNP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..427
                     /gene="tnpA"
                     /locus_tag="OO160_004046"
                     /pseudo
     CDS             1..427
                     /gene="tnpA"
                     /locus_tag="OO160_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(545..1489)
                     /gene="hypT"
                     /locus_tag="OO160_004047"
                     /gene_synonym="qseD"
     CDS             complement(545..1489)
                     /gene="hypT"
                     /locus_tag="OO160_004047"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5525945.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1834..2553
                     /locus_tag="OO160_004048"
     CDS             1834..2553
                     /locus_tag="OO160_004048"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5525946.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2619..3917
                     /locus_tag="OO160_004049"
     CDS             2619..3917
                     /locus_tag="OO160_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5525947.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1048 a          899 c         1025 g         1186 t
ORIGIN      
        1 tggaactgta aatatcacat agttttttgc gccaaaatac cgaagacagg tgttctacag
       61 agagaagcgt agagcaacag gcagtatttt gagaaagctg tgtgagtgga aaagcgtacg
      121 gattctggaa gctgaatgct gtgcagatca tatccatatg cttgtggaga tcccgcccaa
      181 aatgagcgta tccggcttta tgggatatct gaaagggaaa agcagtctga tgctttacga
      241 gcagtttggt gatttgaaat tcaaatacag gaacagggag ttctggtgca ggggggacta
      301 cgtcgataca gtgggtaaga acacggcgaa gatacaggat tacataaagc accagcttga
      361 agaggataaa atgggagagc agttatcgat tccctgtccg ggcagcccgt ttacggaccg
      421 taagtaacga agttggatgc aaatgtcaga tcgtgtgcgc ctgttagggc gcggctggta
      481 agagagcctt ataggcgcat ttgaaaaacc tccggctatg ccggaggata tttattgcac
      541 gtcgtcaatt gcggcgtgct ggctcttcca ttgatatctg ctggattaaa tcgtctggca
      601 tatgttcctg taaaatacgc cagaggttct cggcggttct attcagtcgg gtgttcattc
      661 ggtaaatata agcctgaatg gggactgtca atttagcagc atcaaggcat atcagacgtt
      721 tggtctgtag ttcatcgacg atcgaccaga tcggtaacca ggcaatgccg tatccgttga
      781 gcgccatatt ctttaataac tcgctcatcg aagagataaa tatcgtgcgc ccggtaatac
      841 ctccaacctc tgccagatga cgattgacca acctgcccat ataagatgtg gcggtatagt
      901 tgagcaaagg aacctggggc tgagtgatat cgaatacagg gtgcccctgt gcgtcggcgg
      961 cacataccgg gtacaactca gattcaaaca gtttcaggca gcaaaaaggc gattgcataa
     1021 gatcttcatc gcgaaacgaa ataataaagt cactcttgcc ttcgcgtaac gtatttacgg
     1081 cctggacaac atcaatggct tcaacatgat agacaaattc tccgccatag gcggtaagtg
     1141 aatgcaccag tttgggtaat acgctcaggg atagtgagtg cgcggctgca attttaatgt
     1201 tgggaacgcc gagtaaactc tggccgttta actcccccaa attgcattca agctgctgta
     1261 ataagctgcg agtctgggaa tgaaagagct tgccctcttc ggttaattgt aaaggggtgg
     1321 tcgtgcgatc gaataaaaca acgccgatcg ctgcctccag cgccttaatt cgtcggctga
     1381 atgcgggttg cgaaagatta cgctcttttg ccgcctgaga aaaatggcga caggcttcca
     1441 gtgtcaggaa atcgtataac cattttgtct cgatattctt cattaccatg gcacctgtat
     1501 tttcaatcca aagacataat aaaacatccc ggcaataaaa ataatgttct caattagcac
     1561 aacctggcct ttatcattcg ttttgtgcat aaccccttca tttccggtat tgacgttgat
     1621 atcacatttc gccaaaaaaa cgaaattttt ccgtcagata accagaatac atcatcacta
     1681 tggtgaataa gcggaatgtt ccatagctgc atcatgcgtg tcttgcatcg ccttgtacaa
     1741 ccttgcattt ctcatcgccc atttcgactg ttaaaaaaga gagcagcagt tatttaccgc
     1801 gtattgtcat taacgttttg agaggcaggt attgtgaaag gtttaattgg tgtactcggt
     1861 ggaatggggc cggcagctac cgtcgacctg tttaataaat ttgtaacttt taccgctgca
     1921 caatgtgatc aggaacatat tcctttaatt atttcttcga tcccggatat tcctgaccgt
     1981 accgatgcat taatgcatca tgggcgttca ccattacccg ttatgcgtga ttatatgcat
     2041 aagctggaag atgccggggc agaatgtatt gttattccct gtaataccgc gcattactgg
     2101 ttcaaagaat taaaagatgc ttgtcatatt gatatattaa gtatcgtaga aactacgatc
     2161 aatgaagttc gcgcctgtgg taaaacccga attggcttgt tagccaccaa tgccacgcta
     2221 tatatgggtt tataccaaaa aggaattgag tcgctgggat ttacctgtgt cagccccgat
     2281 gccgacggtc agaaaaaagt gatggaaagt atttattcct tgaaagctgg caatatagcg
     2341 cacgcacaaa agttgatgaa tgagcaggct gaaatattat tttcccgcgg tgcagaaatc
     2401 cttgttcttg gttgtacaga aattccggtt attctggcgc aagccgttaa agagcagcct
     2461 tcccgctata ttgactcaac ggcgtcactc gttcgtgccg gaataaaatg gtacgaaaat
     2521 cgtgtcggta aacattatct tttaacccaa taatcattta aatcgcagcc agaaagttat
     2581 tcgcttttaa ctgaattata tttataacgg agaacattat ggtttggctg gaaattatcg
     2641 tagtacttgg tgcaatattt tttggtattc gccagggggg aatcggtatt ggtttatgtg
     2701 gcgggcttgg gcttgccatt ctgactctgg gatttggtct gcctatgggg tcgccgccag
     2761 tcgacgtgat cctgattatt atgaccgttg ttgtggcggc ctcagcctta caggccgcag
     2821 gcggaatgga ttatctggta cgcgtcgctg gtaattttat gcggcgtaac ccgaaatata
     2881 tcaacataat cgcgccgatt atcacctggt ctatgaccat tatggccggt actgggttta
     2941 ttgttttttc cacgctgccg gtgattgctg aagtggcgaa agagtccggt atccgtcctt
     3001 cccgcacgct tgccggttcg gttgtcgctt cgcaggttgc gatttccggt tcgccgatca
     3061 gcgccgcaat ggcggctatg ctcactatta tggaggtaaa cggtgtcagt tttattcaag
     3121 tgatgtcggt atgtcttccc acctcttttg ctgccgcgat ggtcgccgct tttattgctt
     3181 cccgacaggg atgtgagttg caggatgacg aggtctacct tgagcgcctg caaaaaggac
     3241 tggtgcaaaa atacgagaat aatagcagca tcaagccagg tgcgacgctc tccgtcggat
     3301 tgttcctgct ggcgacggtc gctattgtta ttcttgctgc cttcccccaa ctgcgtccgg
     3361 gttttgatat cggtaaaccg atggaaacac gcgatatcat tattatctgt atgctctctg
     3421 ccgcctgcct gatggtggtg ctgtgtaaga cggcgaccga tgccattatt ctcacctcca
     3481 cattccgtgc ggggatgagt tcgctggcgg taattctcgg cattgtaacc ttaggcacca
     3541 cttttattga tgcgcattta actgagatta aagatattgc tggtgatatt ttgcaggcgt
     3601 atccgatgtt gttggcgctg gttctttttg gcacttgcgc gctgctctac tcgcaaggtg
     3661 cgaccacgcc gttaattatc ccgcttgccg tcgcgcttga cgtgcccacc tgggcaattc
     3721 tggcgtccta tgtggccgtc accggagtat ttgtactgcc aacgtatcca acatcactgg
     3781 ctgccatgga atttgatact accggcacaa ctcgtgtagg caaatatgtg ctgaatcatc
     3841 catttatgct gcctgggttg gggggaatta ttgctggagt ggcattaggc tttgtgattg
     3901 cgccaatgat tatttagcac tatcaaacat cagtatcgat ggtttataca caataaagac
     3961 agatatggcg gaatatagtc tgttgtaagc gccaacaaag aataggggaa ttccgccatg
     4021 tctgaacatc ttcacaatat ggtattaagt gttgtcgttt aatgatttat ttcttggtta
     4081 tgttgtgtat tagctgcatc tatcgctttt tgatacctga ggtgtactgg caatagcgga
     4141 cactaccatt tgttcttt
//
LOCUS       DAJTNP010000200         4051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTNP010000200 DAJTNP010000000
VERSION     DAJTNP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(349..996)
                     /gene="ychE"
                     /locus_tag="OO160_004050"
     CDS             complement(349..996)
                     /gene="ychE"
                     /locus_tag="OO160_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5525948.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1473..>4051
                     /gene="adhE"
                     /locus_tag="OO160_004051"
                     /gene_synonym="adhC"
     CDS             1473..>4051
                     /gene="adhE"
                     /locus_tag="OO160_004051"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5525949.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILL"
BASE COUNT         1122 a          991 c          929 g         1009 t
ORIGIN      
        1 caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac agttccagta
       61 gtgaaatgca caataattta acaattgatt attatttagc actttcctct accaaaggca
      121 gtggaattac taatatcatt tcaattattc ttcagcaagc ccaggattat gatgtggcga
      181 aaataacatg atggtgagga gaagcagtta aggaaatcct tataactaac tgtaatttga
      241 gaagaatatt tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat
      301 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
      361 gccggggaaa atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa
      421 tagccccata atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca
      481 cggtgccatg cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa
      541 accaaacaga tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc
      601 ccctggcccc gccatcaacg gcaacgccaa tggcaccaca ccaatacttt cacgtaccgc
      661 ggtttctgat ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga
      721 catcgctatc gtcaccacca ggatgccccc ggcgatacgg aacgaatcaa ttgatatacc
      781 aaaaagttgt agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga
      841 caggttggct gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct
      901 gataaagacg ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt
      961 gaagtaaacg ggaaaatcaa aaagggtctg aatcacggtt cgctccgaag caaaagccgg
     1021 ataatgttag ccataaataa ggttgaaaag acgcgctgac aatacgcctt ttgacagcat
     1081 ttttcacctc ctaactactt aaaattgcta tcattcgtta ttgttatcta gttgtgcaaa
     1141 acatgctaat gtagccacca aatcatacta caatttatta actgttagct ataatggcga
     1201 aaagcgatgc tgaaaggtgt cagctttgca aaaatttgat ttggatcacg taatcagtac
     1261 ccagaagtga gtaatcttgc ttacgccacc tggaagtgac gcattagaga taataactct
     1321 aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt
     1381 cattggttaa gcaaatcatc accgcactga ctatactctc gtattcgagc agatgattta
     1441 ctaaaaaagt ttaacattat caggagagca ttatggctgt tactaatgtc gctgaactta
     1501 acgcactcgt agagcgtgta aaaaaagccc agcgtgaata tgccagtttc actcaagagc
     1561 aagtagacaa aatcttccgc gccgccgctc tggctgctgc agatgctcga atcccactcg
     1621 cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga agataaagtg atcaaaaacc
     1681 actttgcttc tgaatatatc tacaacgcct ataaagatga aaaaacctgt ggtgttctgt
     1741 ctgaagacga cacttttggt accatcacta tcgctgaacc aatcggtatt atttgcggta
     1801 tcgttccgac cactaacccg acttcaactg ctatcttcaa atcgctgatc agcctgaaga
     1861 cccgtaacgc cattatcttc tccccgcacc cacgtgcaaa agatgcaacc aacaaagcgg
     1921 ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc gaaagatctg atcggctgga
     1981 tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca ccacccagac atcaacctga
     2041 tcctcgcgac tggtggtccg ggcatggtta aagccgcata cagctccggt aaaccagcta
     2101 tcggtgtagg cgcgggtaac actccagttg ttattgatga aactgctgat atcaaacgtg
     2161 cagttgcatc tgtactgatg tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac
     2221 agtctgttgt tgttgttgac tctgtttatg acgcagtacg tgaacgtttc gcaacccacg
     2281 gcggctatct gttgcagggt aaagagctga aagctgttca ggatgttatc ctgaaaaatg
     2341 gtgcgctgaa cgcggctatc gttggtcagc cagcctataa aattgctgaa ctggcaggct
     2401 tctctgtacc agaaaacacc aagattctga tcggtgaagt gaccgttgtt gatgaaagcg
     2461 aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat gtaccgcgct aaagatttcg
     2521 aagacgcggt agaaaaagca gagaaactgg ttgctatggg cggtatcggt catacctctt
     2581 gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga
     2641 aaacagctcg tatcctgatt aacaccccgg cttctcaggg tggtatcggt gacctgtata
     2701 acttcaaact cgcaccttcc ctgactctgg gttgtggttc ctggggtggt aactccatct
     2761 ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac cgttgctaag cgagctgaaa
     2821 acatgttgtg gcacaaactt ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg
     2881 cgctggatga agtgattact gatggccaca aacgtgcgct catcgtgact gaccgcttcc
     2941 tgttcaacaa tggttatgct gatcagatca cttccgtact gaaagcagca ggcgttgaaa
     3001 ctgaagtctt cttcgaagta gaagcggacc cgaccctgag catcgttcgt aaaggtgcag
     3061 aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct gggtggtggt tccccgatgg
     3121 acgccgcgaa gatcatgtgg gttatgtacg aacatccgga aactcacttc gaagagctgg
     3181 cgctgcgctt tatggatatc cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag
     3241 cgaaaatgat cgctgtcacc accacttctg gtacaggttc tgaagtcact ccgtttgcgg
     3301 ttgtaactga cgacgctact ggtcagaaat acccgctggc ggactatgcg ctgaccccgg
     3361 atatggcgat tgtcgacgcc aacctggtta tggacatgcc gaagtccctg tgtgctttcg
     3421 gtggtctgga cgcagtaact cacgccatgg aagcttatgt ttctgtactg gcatctgagt
     3481 tctctgatgg tcaggctctg caggcactga aactgctgaa agaatatctg ccagcgtcct
     3541 accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt tcacagtgca gcaactatcg
     3601 cgggtatcgc atttgcgaac gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg
     3661 gttcccagtt ccatattccg cacggtctgg caaacgccct gctgatttgt aacgttattc
     3721 gctacaatgc gaacgacaac ccgaccaagc agactgcatt cagccagtat gaccgtccgc
     3781 aggctcgccg tcgttatgct gaaattgccg accacctggg tctgagcgca ccgggcgacc
     3841 gtactgctgc taagatcgag aaactgctgg catggctgga aacgctgaaa gctgaactgg
     3901 gtattccgaa atctatccgt gaagctggcg ttcaggaagc agacttcctg gcgaacgtgg
     3961 ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg cgctaacccg cgttacccgc
     4021 tgatctccga gctgaaacag attctgctgg a
//
LOCUS       DAJTNP010000201         4043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTNP010000201 DAJTNP010000000
VERSION     DAJTNP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(886..1077)
                     /locus_tag="OO160_004052"
     CDS             complement(886..1077)
                     /locus_tag="OO160_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5525950.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="OO160_004053"
     CDS             1142..1465
                     /locus_tag="OO160_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5525951.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO160_004054"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO160_004054"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO160_004055"
     CDS             2143..2673
                     /locus_tag="OO160_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5525952.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTHWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO160_004056"
     CDS             2680..2913
                     /locus_tag="OO160_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5525953.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3460..3852)
                     /locus_tag="OO160_004057"
     CDS             complement(3460..3852)
                     /locus_tag="OO160_004057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525954.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          918 c         1208 g          951 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 cactggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggg caccgggaac gcgcttcagt cagaccttgc
     3121 tgaaaacaca ggaacgagtt aaaacgcata cgggcgcgat tctggtgtgt cttttgtcgc
     3181 tgtgttttgc ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctca
     3241 ggtccccgtc ttcttcgctt ccgaaagcat gaataaaatc cgtttcagct gtctctggct
     3301 ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg
     3361 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     3421 cgcgccgaac gcgtttcagc ggtgcgtaca attaagatat tacgttaaat tgtgagggaa
     3481 tggtccccat atactgactt tatcataggg atagggttgg tagtgggtcg attgggtttc
     3541 ggcattgttt ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga
     3601 ttctcctggg cctggaaagg ctaagtgttt agaattgatg gcaaaatatc ttccgtccga
     3661 tttttgaagt agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat
     3721 ttgtagtttt tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg
     3781 tattattctc tcttttaata agctgtttac cacagggttt agttgttgtc taaagcacgc
     3841 agataatgcc attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg
     3901 tgtttgatgt gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac
     3961 ggctataaaa caaaattttc atagagtgat tcaagatctt cttgagatcc ttttttgcgg
     4021 cgcgtaatct gctgcctgca aac
//
LOCUS       DAJTNP010000202         4029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTNP010000202 DAJTNP010000000
VERSION     DAJTNP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(151..1008)
                     /gene="sitD"
                     /locus_tag="OO160_004058"
     CDS             complement(151..1008)
                     /gene="sitD"
                     /locus_tag="OO160_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5525955.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1005..1862)
                     /gene="sitC"
                     /locus_tag="OO160_004059"
     CDS             complement(1005..1862)
                     /gene="sitC"
                     /locus_tag="OO160_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5525956.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1859..2686)
                     /gene="sitB"
                     /locus_tag="OO160_004060"
     CDS             complement(1859..2686)
                     /gene="sitB"
                     /locus_tag="OO160_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5525957.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2686..3600)
                     /gene="sitA"
                     /locus_tag="OO160_004061"
     CDS             complement(2686..3600)
                     /gene="sitA"
                     /locus_tag="OO160_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5525958.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1088 a          983 c          961 g          997 t
ORIGIN      
        1 actttcgata gtgcgagtat tgaatgattt ctaggcgtta tcgattttac gtattttttg
       61 catgatagaa ttcgtacctc ctcccaacga ccttccatgg ctttccggac ttctgtaggc
      121 tggcggacga tgctgtcctc ggatgttgtg ctaattgggt gataacagat cctgtgtatg
      181 ggcgtttccc tttgtgcgtg cgttgatgct ggtaacggta aacgtcatga tgaacacgac
      241 cgcgaataag acgacaatcg tcggagctgg tgcgctgtcg ataaaaaagg acagccagac
      301 accagtcatt gacactatta ccgatatgcc agtcgccagt aggagcgcaa tatgaaagcg
      361 ttgtgtcagg agtactgcaa tggcgccagg cgcaatgagc agagaaatag agagaatgat
      421 acctactgct ttcagcgtcg ccacaatggt cagggaaacc atgcacagca gcccataatg
      481 cagccatctc gtatgcaaac cgcttacctg cgcctgttga taatcaaagc tgaacagcaa
      541 aaaatcccgc cattttacgc taataacaag tgtaaccagc ccggcaataa tcaccgtctg
      601 gattatgtcg ccgatggtta tcccgagcat gtcgccgaaa agaatatggt caagatgtac
      661 gtctggttta attgcaatat acaagatcag acctgcggca aacatgccag aaaaaacaat
      721 ccccataact gtatcttgct tgattcggct gttgtctttc aggtatcccg tcgccaccgc
      781 gcagaatact ccggcgacga atgcccctgt agccagaggt aaccccagaa tccaggtaag
      841 cacgatgcca ggaaaaaccg catgactcat cgcgtcaccc atcagtgccc aacctttcag
      901 gaccagaaag accgacagca gcgcacaggg tatggaaact accagtgaaa tcagcagtgc
      961 gtgactcata aaggtgaact gcagtggctc cagtaataat gccatcatga gcgatcctcc
     1021 agcgctttat gcgcacgccg acgatttgcc aaaagtccat gggtgggcgc aaatacaaat
     1081 gctaaaagaa acagcagtgt ctgcgccacc acgatgattc cacctgtcgc gccgtccaga
     1141 aagtaactga cccacgcgcc gaggaaactg gttacactgc caatagttac agcaattatg
     1201 agcaggcggg gaaaacgatc ggttaacaac catgctgttg caccgggagt caccaccaga
     1261 cagatcacca gaaaggcacc aaccgtttgc agcgccgcaa cggtcgaaac cgacagcagg
     1321 gtgaagaaga ggatttttaa tctgcccgga tgtaatccaa tagcacgggc gtgattctcg
     1381 tcgaaaaagg tcaccataag atctttccat ttgaaaaaaa gcacgatgat cgagagaatg
     1441 ccaatgatag tgagttgcag gatatccgcc ggatcgattg ccagaatatt gcccaggacg
     1501 atggtctgaa tgttcacgga tgtcgggttc aatgacacca taaacagccc caggccaaaa
     1561 aaggacgaga agatcaggcc gatgatggca tcttctttta agcgggtgcg ttggttaaga
     1621 aacagcatgc tgcccgctgc cagtccgccg gaaaagaagg ctcccagaga aaacggaagc
     1681 cccagcatat atgcccccgc aacacccggg acaatagagt gcgacagcgc atcgccaatc
     1741 agcgaccagc cttttagcat cagatagcat gacaggaaag cgcagagacc gccgaccatt
     1801 gccgagaccc acatcgcatt gagcatatat tcatagctga agggttccag tagcacgttc
     1861 atcttgcttc cctctgcacc gctgacggtc gatgtgccac aaaagggcgt tcatcgtcag
     1921 taataatgct ttcttccgag ccgttaagtg tgacatggcg cagtacgccg ctaaaagcca
     1981 gctccaggtt ctcggctgta aaagtggtgt ctgtcggccc gcttgccagg acggtgcctt
     2041 tgaccatgac cgtataatcg caaaatgtcg tgactgagcc gagattgtgc gttgagacaa
     2101 gcatcgtttt gccttccgcg cgtaactccc tcaacaggct gatgattttc gcttcggttt
     2161 tcacatcaac gccggtaaaa ggttcatcca gcaggatcac atcaccctgc tgtgcaattg
     2221 ctctcgccag aaagacgcgt ttcttttgtc caccggaaag ctcgccgatt tgacgatggc
     2281 gaaaatccac catatcgaca cgttccagcg catcggtgac aatctgacga tccctctttt
     2341 tggctatacg caacataccc atatgcccat agcggcccat catcaccaca tcttccacca
     2401 gtacaggaaa tgaccagtca acctcttctg actgagggac gtaggcaacc aggtttttct
     2461 gtagcgcctg tcgcgtggga atacccagaa cagatatctt tccgctggtc agacgcacaa
     2521 atcccataat cgctttaaac aacgtcgatt ttccggaacc gtttacccca accagagcgg
     2581 caatagatcc acttggtact gtgaaggatg catcgcgcag tgctgtgtgc ccgttacgcc
     2641 aggtgacagt aacatcatta acgacaatgc ctgcagattg catcattatt tctccctttt
     2701 cccggcttta attccctgta ccagcgtact ggtagtaacc ttaagaaggt cgatatacgt
     2761 aggtaccggg ccgttttctg tgctcaggga atcgacatag agcacaccgc cgtagtgtgc
     2821 gccggtttca cgcgcaacct gacgcgctgg tttatcggaa atcgtactct cgctaaagac
     2881 tgccgggata tgatttttct taactatatc aacaacctta cgtacctgct gcggtgttcc
     2941 ttgttgatcg gcattaatcg gccacagata aagctctttt agccccaaat cgcgtgcgag
     3001 ataagaaaaa gccccttcac tggtgaccat ccatcgctga ttctcaggga gttccgtaat
     3061 ctgcttacgc aggggggcaa gggtttgggt aatcttggct ttataagtat cggcattacg
     3121 ttggtaggtt tgtgcatttg ccggatcgta ttttatcaac gcatcacgaa tattatcgac
     3181 gtaaatcaga gcattatctg gcgacatcca tgcatgggga ttaggtttgc cctcataggg
     3241 tccttcggtg atccctactg gcgtcacacc cgaagagaca attacttctg gaaccccatt
     3301 gagatgctgg taaaagcgtt ggaaccacaa ttccagattc ataccattgg cgagaatcag
     3361 ttgtgccccc tgcgcacgtt taatatcgcc aggggtaggc tgatactcat gaatttctgc
     3421 accaggcttg gttatggatg agacttctgc agcatctcca gccacgtttt tggccatatc
     3481 cgcgatgatg gtaaatgttg taatgacctt gaatttttca gttgcacttg cctgaaatgc
     3541 gatcgagcag gtgagggcga ggcaccccaa gagcatggtt acttttttta ttgagtgcat
     3601 aatttagtac ctatagtgag caaaattaga acaaacatag caacggctat gttttatagc
     3661 ataacctata caaaatgaaa ataattatca ttaacagctt tgaggcctaa aacagggatt
     3721 aggttgtttc ctgctggcga catgatattg aggaaaaaag tagagcagct attaggcttt
     3781 gaaatgactt tatgaggaaa attgttgatg gcaaaaatgg ggcaaaaaag agtgggttat
     3841 cgtagcttat tgttgtcgct gataatgttt aacatattga aaaataaata aaatacttat
     3901 gagtcagata gttgtgattt ttgcccttac ttgttcaggt tgtattgttc tttctttgta
     3961 atttgttgat tttcttgcat tatttcagtt ctctggtact aaatggggca aattgggggc
     4021 aaactttgc
//
LOCUS       DAJTNP010000203         3902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTNP010000203 DAJTNP010000000
VERSION     DAJTNP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(96..257)
                     /locus_tag="OO160_004062"
     CDS             complement(96..257)
                     /locus_tag="OO160_004062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525959.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(254..442)
                     /locus_tag="OO160_004063"
     CDS             complement(254..442)
                     /locus_tag="OO160_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5525960.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            702..1037
                     /gene="iraM"
                     /locus_tag="OO160_004064"
     CDS             702..1037
                     /gene="iraM"
                     /locus_tag="OO160_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5525961.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1318..1426)
                     /locus_tag="OO160_004065"
                     /pseudo
     CDS             complement(1318..1426)
                     /locus_tag="OO160_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1949..2025)
                     /locus_tag="OO160_004066"
     tRNA            complement(1949..2025)
                     /locus_tag="OO160_004066"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1989..1991),aa:Arg,seq:tct)
     gene            complement(2127..2202)
                     /locus_tag="OO160_004067"
     tRNA            complement(2127..2202)
                     /locus_tag="OO160_004067"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2167..2169),aa:Ile,seq:cat)
     gene            complement(2347..3168)
                     /locus_tag="OO160_004068"
     CDS             complement(2347..3168)
                     /locus_tag="OO160_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5525962.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3183..3539)
                     /locus_tag="OO160_004069"
     CDS             complement(3183..3539)
                     /locus_tag="OO160_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5525963.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3552..>3902)
                     /locus_tag="OO160_004070"
     CDS             complement(3552..>3902)
                     /locus_tag="OO160_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5525964.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1099 a         1048 c          749 g         1006 t
ORIGIN      
        1 tactgatggc tctgataact catgcaagcg tctcagacga cccactgaca ctaccggtga
       61 gtttaacgat accttccatt tgactggctc actttttatg atgatgccgg tgcatttatc
      121 tccagcacca gactttctat ctcaacgcca tacgttgcat ttttggtaat atccgtcagc
      181 gtcagtgcat ttagtcccac tgccagactg tcttttatgg cctggaatgc cgggccagta
      241 cgatgacgta gtatcactcc ggctcagttg caccactgac caccacatca ccttctgctg
      301 caatcgcctg catcagggta taaggggtta tggccaccgg actaccaaac ggctgccagc
      361 cctctttcag tttatgtgtc agcttttccg caagatctga cggcggcgcc gccctgacaa
      421 catcatagtg tttaaatgcc atggttcttt ccaacatctg aaaaataatt ctttaaaata
      481 cctgacatgt aatacagaaa aaacacaaaa ccatacctta aataaaaacc tgattatcaa
      541 gcagatatgc atggataaac tacaagacga gatataaacc accctgcatt taaataaaca
      601 ataaacaaca tcagaaaaat aattctgttc tatggtttaa ttcaaaaata tcatttatac
      661 ttttcagaac atcaccagca aggcataaac aaggaaacta aatgaagtgg attgtgattg
      721 atacagttat ccagccatca tgcggaatat ctttttcagt catatggagt aaaataaaat
      781 taataatctg gtatcaatcg gatgctttct tacctcctga aagtatattt acactgactc
      841 acacaggcat catgctcaat aacaaagtgc tacctgtaac catttacaac gtagtaccat
      901 tcaataaaac attctggaat ttaatcaaaa acagccagga atgccctaca aatacagata
      961 acgtattgaa tgaatgcttt aataaccgtt gcactctgca aatatgccct tatggactaa
     1021 aacaacaaag tccataagga gtttactcac atctgacaaa atcaatataa acagcccctc
     1081 cggagagggg ctggagagtg gcgctatgtg ccattccatg gtgccgggtg cctcccggtg
     1141 aattcagtac cagcacctga atccgcgatt atcccatata cctactcgct gattgcccct
     1201 ccgcacaggg ggattcacca tgccagtttc ttttaacaaa ctccccgcaa accagacaac
     1261 agtcaaccgc ctgaattgtg aagtatttaa aaatttctcc cgctaactga tacccggcta
     1321 acagtctggc gttttctttt tcagcaacgg gaaagcaaca accaccacac ccgccaccag
     1381 cacaccgtca gccggcactg acattatccg gctgctgcaa tgccattaac aaaaacagta
     1441 agcaatcact ttttaccgta acaggtgata atccagatat gtatctaccc cagatgagta
     1501 atccgaagtt catccatacc acaggtcctg gctattctgt tgtactcctg aacaagagca
     1561 aataattctg aattagcaac catgaactca tcgcaaaccc tctgtatagc atcactattc
     1621 agaataataa cgtctcttcc cgaaagacga tcaggagtac agaacaaaac tgtcaaacgg
     1681 ctgaaggcct ttgctcgtgc tgcattgact atatcaatac gctgcctaag gatgaaacac
     1741 cccgacgcct catcaatatt cactctaccc acaccatatg aatgataaat acttaatgct
     1801 gaaaaaacca ttagaccgta taacaaatac tcaatcaata cttaacagaa cttttatttt
     1861 tgacaaatat ataatatttt caacaatatc ctgagccagg tatatttcag tataaggctc
     1921 tgccggaagg aatctggaag aatgaatatg gcgcgctgta ctggattcga accagtgacc
     1981 gattgcttag aaggcaattg ctctgtccgg ctgagctaac aacgctgaat accgataatg
     2041 gaccgccatc ggggacccgc cccccgcacc aacaaccctg ttatcgtgtc gtctgctctt
     2101 cctgataagc taatggcggt ttgtgatggt ggcccttgct ggatttgaac cagcgacctg
     2161 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgggagcag aataataacg
     2221 gtccgtaatt aatttcgcaa taaaaaaacc cgctcagcgg cgggttgtag aaactcttct
     2281 aacgccaggc ataaaacgcc catcattatg acgaatttac cacagattcc ggaaaaatca
     2341 accttgttac ctagttacct tttttaactg ccgctcagcc catgcttctt caatatcaaa
     2401 ccgggtcacc agcgcatcat aaaatttctt aactgttttt tcccatgacg cgcgtgttat
     2461 ctggtttgtc acctcgcata tagcattaaa tgcctccgtt gatggtagtc tttcatagcc
     2521 acgaccacca caacgctggc agtctctgat aacaggcata ccacgtttta ccgactcttc
     2581 acgatgaatg gcgacaccac gcccacggca atccttacag gcggtggaaa cctcaccctt
     2641 tccgccacac tccggacagg caacttttac cacctccctg acttttttcc attcttccca
     2701 gtaaaacgga tacacacctt tcgtacactt tgcccatacc ggcggcttac catccggata
     2761 ctggatcttg tttgtaaaaa ctatgctttc aataaatttt tccccatagc aacaagggca
     2821 ctgcttttta ctcgctgcgc tgcgggcata atcctcaaaa gcgtacgaag ccataatgcg
     2881 catcactacc ggttttattt ctgccggaag ttttctcaac gccgccacac gatcgcaccg
     2941 actgagtgca taatctgcca gtaattctgt tgcccgcgcc ctgtcattca tactgatgcc
     3001 cattttcccc aggaacgcag aaaaacccat ctcagcccga ttctgtgtca tgccctgcgc
     3061 ggccatcaca tcagtgatac tcagcgcatc ttttgacgtt gaggccgatg catcggtcag
     3121 gccaggggat tttggggagt agtatttcgg taaatcttcc agtttcattt tttgacctgc
     3181 tcttaatgca ttatggggta aatcttcacc cccagacgtc caccagatac tggctgacca
     3241 cgaacgatat tgatttcatc aaactgctca tcgtccatta acactcccgc atgcgtcagc
     3301 gcatccagcg gtgctttcag gatattgtcc aggtcgcgac gacgcttatc cggtggctct
     3361 gcaatcacct ttatcgccag ccttccggac aggcttaatt tcagccgctg ctggcgaaca
     3421 ataagcgcca cagcccggcg ataacgcttt ccctcctccg agataaaata tgtgctgcca
     3481 cggcgtcgcc agtaagtgtt caccgtcggc gggtaaggta aaaccaaatc tatgagcatc
     3541 agtcacctct tttacccaag cacgccagtt gcaaaggcgt gatcaagaaa acgaaaaatt
     3601 aaatcaacct gagaaccatg cttttcttcg aacgccagag gatccgcatg aagctcgttg
     3661 tgatgctccc gacacagcgg tagcgtgaaa atatcgtgag atttggtccc cattccgccc
     3721 tgaccatgac caatcaggtg atggggatcg tcggctggct taccacaaca cgcacacggc
     3781 tgtgtcttca cccagcgttt gtatttctcg ttaacccagc ggcgacgttt aggtcgtttc
     3841 atgaaagatt ccggagactc aggatcaacg gcaatgctga ccaccgtctt ttcctgttgt
     3901 gg
//
LOCUS       DAJTNP010000204         3881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTNP010000204 DAJTNP010000000
VERSION     DAJTNP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            180..437
                     /locus_tag="OO160_004071"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO160_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO160_004072"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO160_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5525965.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO160_004073"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO160_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5525966.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO160_004074"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO160_004074"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5525967.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO160_004075"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO160_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..>3881)
                     /locus_tag="OO160_004076"
     CDS             complement(3595..>3881)
                     /locus_tag="OO160_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5525968.1"
                     /translation="DNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTLLDD
                     GYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
BASE COUNT         1013 a          919 c          916 g         1033 t
ORIGIN      
        1 cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3781 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3841 tgtacggcta ctgacgttaa ttttatccgc tagattatcc a
//
LOCUS       DAJTNP010000205         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTNP010000205 DAJTNP010000000
VERSION     DAJTNP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            437..2089
                     /locus_tag="OO160_004077"
     CDS             437..2089
                     /locus_tag="OO160_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5525969.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO160_004078"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO160_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO160_004079"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO160_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO160_004080"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO160_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTNP010000206         3725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTNP010000206 DAJTNP010000000
VERSION     DAJTNP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..723
                     /locus_tag="OO160_004081"
     CDS             <1..723
                     /locus_tag="OO160_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5525970.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVART
                     VLRAVRDTVVSVARAVWNWIFF"
     gene            1095..>3725
                     /gene="ag43"
                     /locus_tag="OO160_004082"
     CDS             1095..>3725
                     /gene="ag43"
                     /locus_tag="OO160_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5525971.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
BASE COUNT          854 a          964 c         1121 g          786 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
      241 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
      361 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
      541 gacgagctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg tacggttctg
      661 cgtgccgtcc gtgacacggt ggtctctgtt gcccgcgctg tatggaactg gattttcttc
      721 tgaacagtct gccagggaaa tgcctgataa cattacgtta accaactgaa aacatgacac
      781 aaacccctca tactcctcct gtttctcttg cgtgaccgcc ttgtcgttaa tagaataaaa
      841 ctatcgataa aacagatatc gatcgttgct atcgatcgtt tatagtggtt aatggtctgc
      901 gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaacg gactgatact
      961 tctctggcgg atgagagagg ggaagcattt atgtccggga aaaaccactt tatctgacca
     1021 tgctgttttg tacctgccgg tatccacttt tgtgggtacc ggctttttta ttcaccctct
     1081 gtaaggaaaa gctgatgaaa cgacatctga acaccagcta caggctggta tggaatcaca
     1141 ttacgggcac cctggtggtg gcttccgaac tggcccgctc acggggaaaa cgcgccggtg
     1201 tggcggttgt gctgtctctt gctgctgtca catcagtccc ggcactggct gctgacacga
     1261 ttgtacaggc gggagaaacc gtgaacggcg gaacactgac aaatcatgac aaccagattg
     1321 ttctcggtac ggccaacgga atgaccatca gtagcggtct ggagtatggg ccggataacg
     1381 aggccaatac cggcgggcaa tggatacaaa atggcggtat cgccaacaac actactgtca
     1441 ccggtggtgg tcttcagaga gtgaatgccg gaggaagcgt ttcagacacg gttatcagtg
     1501 ccggaggcgg acagagcctt caggggcagg cagtgaatac cactctgaac ggcggtgagc
     1561 agtgggtaca tgaaggcggg attgcaacgg gtaccgtcat taatgagaag ggctggcaga
     1621 ccgtcaaatc cggcgcaatg gcaaccgaca cggttgtgaa taccggcgcg gaagggggac
     1681 cagatgcaga aaatggtgat accgggcagt ttgttcgcgg aaatgccgta cgtaccacta
     1741 tcaatgaaaa tggtcgtcag attgtggctg ctgaaggaac agcaaatacc actgtggttt
     1801 atgccggcgg cgaccagacg gtacacgggc atgcgctgga taccacactg aatggcggtt
     1861 accagtatgt gcacaacggc ggtacggcgt ctggcactgt tgtaaacagt gacggctggc
     1921 agattatcaa ggaaggtggt ctggcggatt tcaccaccgt taaccagaaa ggcaaactgc
     1981 aggtgaacgc cggtggtacc gccacacatg tcaccctgaa acagggtgga gcactggtta
     2041 ccagtactgc ggcaacggtt cttggcagca accgtctggg caatttcacg gtggaaaacg
     2101 gtaaggctga cggagttgtt ctggagtccg gcggtcgcct ggatgtactg gaaggccatt
     2161 cagcgcagaa aacacgggtg gatgacggcg gaaccctggc agtgtctgcc ggtggtaagg
     2221 caacaggtgt caccatgaca tccggtggtg ccctgattgc agacagtggt gccactgttg
     2281 aggggaccaa tgccagcggt aagttcagta ttgatggcac atccggtcag gccagcggac
     2341 tgctgctgga aaatggcggc agctttacgg ttaatgccgg aggactggcc agcaacacca
     2401 ctgtcggaca tcgtggaaca ctgacgctgg ctgccggggg aagtctgagt ggcagaacac
     2461 agctcagtaa aggtgccagc atggtactga atggcgatgt ggtcagtacc ggcgatattg
     2521 ttaacgcagg ggagattcgc tttgataatc agacgacacc ggatgccgcg ctgagccgtg
     2581 ctgttgcaaa aggcgactcc ccggtaacgt tccataaact gaccaccagt aacctcaccg
     2641 gtcagggtgg caccatcaat atgcgtgttc gccttgatgg cagcaatacc tctgaccagc
     2701 tggtgattaa tggtggtcag gcaaccggca aaacctggct tgcgtttaca aatgtcggaa
     2761 acagtaacct cggggtggca acctccggac agggtatccg ggttgtggat gcacagaatg
     2821 gcgccaccac agaagaaggt gcgtttgccc tgagtcgccc gcttcaggcc ggcgccttta
     2881 actacaccct gaaccgtgac agcgatgaag actggtacct gcgcagtgaa aatgcttatc
     2941 gtgctgaagt ccccctgtat acatccatgc tgacacaggc aatggactat gaccggattc
     3001 tggcaggctc ccgcagccat cagaccggtg taaacggtga aaataacagc gtccgtctca
     3061 gcattcaggg cggtcatctc ggtcacgata acaacggcgg tattgcccgt ggagccacgc
     3121 cggaaagcag cggcagctat ggcttcgtcc gtctggaggg tgacctgctg agaacagagg
     3181 ttgccggtat gtctctgacg acaggggtgt atggtgccgc aggccattct tccgttgatg
     3241 ttaaggatga tgacggttcc cgcgccggca cggtccggga tgatgccggc agcctgggcg
     3301 gatacctgaa tctgacacac acgtcctccg gcctgtgggc tgacattgtg gcacagggaa
     3361 cccgccacag catgaaagcg tcatcggaca ataacgactt ccgtgcccgg ggctggggct
     3421 ggctgggctc actggaaacc ggtctgccct tcagtatcac tgacaacctg atgctggagc
     3481 cacaactgca ctatacctgg cagggactta ccctggatga cggccaggat aacgccggtt
     3541 atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc
     3601 acaacgatat gagctttggt gaaggcacct catcccgtga cactctgcgc gacagtacaa
     3661 aacaccgtgt gagtgaactg ccggtgaact ggtgggtaca gccttctgtt atccgcactt
     3721 tcagc
//
LOCUS       DAJTNP010000207         3690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTNP010000207 DAJTNP010000000
VERSION     DAJTNP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..224
                     /locus_tag="OO160_004083"
     CDS             <1..224
                     /locus_tag="OO160_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5525972.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO160_004084"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO160_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5525973.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO160_004085"
     CDS             518..1108
                     /locus_tag="OO160_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5525974.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO160_004086"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO160_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5525975.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO160_004087"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO160_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5525976.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO160_004088"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO160_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5525977.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO160_004089"
     CDS             3457..3648
                     /locus_tag="OO160_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525978.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          998 a          901 c          928 g          863 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 tacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga
//
LOCUS       DAJTNP010000208         3584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTNP010000208 DAJTNP010000000
VERSION     DAJTNP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..219)
                     /gene="flhe"
                     /locus_tag="OO160_004090"
     CDS             complement(<1..219)
                     /gene="flhe"
                     /locus_tag="OO160_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5525979.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVR"
     gene            complement(219..2296)
                     /gene="flhA"
                     /locus_tag="OO160_004091"
                     /pseudo
     CDS             complement(219..2296)
                     /gene="flhA"
                     /locus_tag="OO160_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2289..3436)
                     /gene="flhB"
                     /locus_tag="OO160_004092"
                     /pseudo
     CDS             complement(2289..3436)
                     /gene="flhB"
                     /locus_tag="OO160_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          872 a         1061 c          892 g          759 t
ORIGIN      
        1 gcgaacccgc agtcctgcag gtgtcgggcc gataagctga taacgccacg ccaccagcgt
       61 catcaatcct gaagcaggtt gtcgcgcaga aagaggtgca gacgacatcg actcaccgcg
      121 atgatttagc gtaattccga cactacttgc ctgccacatc ccctccccgg cggcttgcac
      181 cagcagcgga aacaataata tcgctaataa ggttctcatc atttaccgcc aattgtcgcc
      241 gtcatgcgga tatgtcggtt atcagacagt tccagattcg acaggaccac taactgcggc
      301 aagctgcggc gcaggaagcg agacaataat ggtcgcagcg cgtggttcac caacaatact
      361 ggcggcgcac ccagcatctc ctgacgggat agagcttcct gagtttgcgc cagtaaacga
      421 tccgccagcc ctggctccag tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc
      481 agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca ctgctgggta
      541 atcgcccgtc ccaacgccac gcgcacgacg gcgcttaatt catgtggatc gctttggatg
      601 ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca
      661 tcgaggagat tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga
      721 tcttccgtca gctttggcat ctcctgggcg acgcgatcca acagctgttg cgcctcctga
      781 cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt tgctaccacc
      841 gtgctggcct caaccactgt gtacccctga atctgcgcct gttcttttag cgcactttca
      901 atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg taacgtcccg
      961 gcagcggttc cagggttaat cgccagccag cgccccggat aagcatcacc actgccaatc
     1021 tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg
     1081 tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt tcttgcggat actgcgtata
     1141 cggcccaaca actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg ataacccact
     1201 tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac aacggtatta
     1261 ttctctgcca tttttaccgg tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc
     1321 caccaggcca gccccagcaa tccggcagtg aacagcaaaa ataccaggtt cggcattcca
     1381 ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca acataacgct tgggttactg
     1441 aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg
     1501 ccccccgcgg cggtagaaat caccagcgcc ggaatttgtg ccaccagacc gtcgccaatg
     1561 gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccagttgc agcacgccga
     1621 ccagcaaccc gccgacaacg ttaatgacca tgatgaggat cccggcaatg gcatcgccgc
     1681 gaacaaactt acttgccccg tccattgagc cgtaaaaatc ggcttcctga gtcacttcgg
     1741 agcggcgttt tttcgcctca tcttcaccaa tcaatccagc gttaaggtcg gcgtcaatcg
     1801 ccatctgctt acccggcata ccatcgagaa caaagcgcgc acccacttct gctatacgcc
     1861 cggcaccttt ggtaatgacc ataaagttga tgatcacgag aatgacaaac accacgatac
     1921 cgatagcgaa attgccacca acgaggaagt gaccgaacgc ttcgaccacc ttcccagccg
     1981 ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt tgaagccacg ttaagtgcca
     2041 gacgcaacaa catagtaaac aacagaatgg tcggaaacgc agcaaactca agcgtgcgct
     2101 gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag gtaaacaaca
     2161 ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac aggatcaaaa
     2221 tcggtccggc aaggatctgc cattatgtcg atttcaggtt tgcgggcagg cgcagcatcg
     2281 cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggctgc cggcaccgga
     2341 agatgagtag gtgtacaggg cgctgtccac cagccagacg ccagcgtttc agttgccaga
     2401 cccaggccag cacttccgcc accgcggcgt acagttgacc cgggatttgt tgaccaatct
     2461 ccgcatgtcg atacagcgct cgcgccagcg gcggcgcttc aagcgtcggg acgttatttt
     2521 cagcgccaat ttcacgaatg cgcagcgcga ccagccctgc acctttagcg accactttcg
     2581 gtgcgctcat tttgttttcg tcatactgca acgctaccga atagtgggtc gggttattga
     2641 caatgacatc cgctttcggc acatcggcca tcatccgacg ccgtgcggca gctcgctgca
     2701 tctgacagat ccgtccttta acatgggggt cgccttcgct ttgtttgaac tcatcacgaa
     2761 tatcctgccg cgacattcgc agctttttca ggtggctgaa gatttggaaa aagacgtcaa
     2821 atcccaccat tggaatgaca ccaagcacca ccagcagtgc gcatagccct accaaatcca
     2881 tcgcattacc catggcggta atcggtgact cggccattaa gcgcatcatc tgcggccagt
     2941 gatgccagag aaaaaacccc gtcacgctgc caaccaggat ggttttcaaa attgccttaa
     3001 gcaactccgc gctagtctgt gccgagaata tccgtttaat gcccggtagc gggttgagtt
     3061 tggaaaactt cggctgcaag gatttgccgc taaataccag cccccccagc ataaccggag
     3121 aaatgagcgc caccagcacc acgccgctaa tcagcggtag cagcgccagc atggcttctc
     3181 tgatcagcag aataatttgc ccgaggatca gattcgggtc tttgataata ctgtgatcaa
     3241 aatgcagccc agcggagagc atgcccgaca atcgacgggc cagcgacaca ccgccaaacc
     3301 agataacact aacgcccacc agcaaaatca gcagtgaggt cagttcacgg gaacgcggga
     3361 tttgcccctc ttcccgcgct ttttctagtc ggtgaggtgt gggggcttct gttttgtcgt
     3421 cgctctcgtc agacacgtcg ccaatcctgg aaagagtatt aagcgtaaat gatgccagag
     3481 cgcaaagcgt tcaatggttt aagtaagggg caaaacaggc gggatttagg gcgtttatgc
     3541 aaaaataggt tcagttgcag aacgttttgc gaaccatttt ccag
//
LOCUS       DAJTNP010000209         3442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTNP010000209 DAJTNP010000000
VERSION     DAJTNP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..757
                     /locus_tag="OO160_004093"
     CDS             <1..757
                     /locus_tag="OO160_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5525980.1"
                     /translation="REGRGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEAND
                     LTMELSSIKAEELTDNAGIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLEN
                     GTSLVMTGSCGTGKNHLAVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTAD
                     EVIERYASMDLLIIDEVGVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAF
                     ISPRIADRITDGGRNWLSFNWPSYRSRIRGVAA"
     gene            754..2145
                     /locus_tag="OO160_004094"
     CDS             754..2145
                     /locus_tag="OO160_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5525981.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2142..2399
                     /locus_tag="OO160_004095"
     CDS             2142..2399
                     /locus_tag="OO160_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525982.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2494..2694
                     /locus_tag="OO160_004096"
     CDS             2494..2694
                     /locus_tag="OO160_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5525983.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2687..3067
                     /locus_tag="OO160_004097"
     CDS             2687..3067
                     /locus_tag="OO160_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5525984.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3267..3343
                     /locus_tag="OO160_004098"
     tRNA            3267..3343
                     /locus_tag="OO160_004098"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3301..3303,aa:Ile,seq:cat)
     gene            3345..3421
                     /locus_tag="OO160_004099"
     tRNA            3345..3421
                     /locus_tag="OO160_004099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3379..3381,aa:Arg,seq:tct)
BASE COUNT          894 a          759 c          997 g          792 t
ORIGIN      
        1 gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat cacacgcata tactggtgtg
       61 gagcgatcgt aatggcgagg acagagaaga aatttcatgc tgccctgact gcttaatcgc
      121 tgaggccaac gatttgacca tggagctgtc gtccatcaag gcggaagagc tgactgataa
      181 cgccggaatt gccctgcgtt ttcgggactg cgagtttgat aattatctgg aggttaatcc
      241 tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg gagaactggc cagatatgct
      301 ggagaacggt accagtcttg ttatgaccgg cagttgtggt accggaaaaa atcatctggc
      361 tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc agtgtggaga tcaccgacgt
      421 gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat gacagcgaaa aaacagcgga
      481 tgaagttatt gagcgttatg cgtcaatgga tttgctgatc atcgacgaag ttggcgttca
      541 gtttggcagc gcggctgaaa tggctatttt gcaggaaatt atcaacgcca ggtacgaaag
      601 tatcttgccc accattctga tcagtaacct ctcaccggaa gagttgtggg cgttcatcag
      661 tccccgaatt gccgacagga tcacagacgg gggacgcaac tggttgtcgt ttaactggcc
      721 cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa ctccagtatg gcgtaacgat
      781 gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg gagcagatcc tgaagtgatg
      841 gatattctgg ccacactgcc agcggacgtt ttttctgtac gagcgtatca ggatatctat
      901 acaggcatct gcagacaggc ccgtgtttca ggagtgattg accccgtgct gttgtgtaat
      961 gagatgccgg aacttgcccc ggtgattact gataccgggc gtaaaacctg ggtgaagtct
     1021 tcactggagc actatgttgc agcgttgcgg cgcaatgccg cactgcgcga tgcagaaaaa
     1081 acactgaatg aggcgctgca gaaattacgt gatgcgcata cctgtgaagc agctgaagat
     1141 gccctgaagg atgcgcagaa catgatggtc acactgtcga caggaaaggg cgtcattcag
     1201 ccggtacata ttgatgatgt gcttccggaa gtggttgagc gtgttgaatg ccggaatcag
     1261 gggctggaga aatccaggac gttgatgacc ggtattgatg aactggacgc aaaaacaggc
     1321 ggtatggagc ccggagacct ggtattcatt gccgcccgtc cttcgatggg gaaaaccgaa
     1381 cttgcgctgg acatcatcga caaggtgact gagcaggggc atggtgtgct tctgttcacc
     1441 atggagatgg cgaacatcca gattggtgaa cgtatggtgt ctgcggctgg agggatgcca
     1501 gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag actggacgcg tttctcacaa
     1561 ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg accaggcgaa cctggccatt
     1621 gatgagatat gcgcaacaac gaaacaccac ctgattaaat atccggaaac ggcgctggtg
     1681 gtggttgatt atctcgggct gataaaaacc cgaaccacgg ggcgtcatga ccttgccgtg
     1741 ggtgaaatct caaaggggct taaaggcctg gcaaaatccg gtggttttcc gttgattgcg
     1801 ctgagccagc tctcccgcgg tgtggagtcc agacccaata aacgtcccat gaactcagac
     1861 ctgaaaaatt ccggagaaat agaggcggat gcagacatca ttctgatgct ttacagggat
     1921 gaagtgtaca acccggatac gcaggccagg ggcatcgcag aaatcaatat cacgaaacaa
     1981 cgtaacggtt ctctgggaac gatttaccgg cgtttttata acggacattt tctgcctgtg
     2041 gaccaggaaa gtgcacgggt gctttccact cccatgaagc ccggcaatcc gcgcagatac
     2101 agtaataaac gaactgacag cagtaagatg gagcgtttct tttgaacaac cagacaatga
     2161 cttttacccc tgaacaattg cgtaaacacg cgcaggaaat gctgcgtcat gctgaacaac
     2221 tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga tatggtaccc gcgcttcggg
     2281 aactgatgca ggcgaagcat cgcgcacaga aagcagtaga tgagctggtg gactgtgtgg
     2341 cagagctgga aaccagagtc ggaaagtttg aaaaactggt gcaggaggcg ctgcgctgat
     2401 gcgccatgag tttgttttgc cctggcttgt gcgcgatcat tatgtccctg gtggaaagcc
     2461 cgggataaaa atcaccgaac cggagagtat ttgatgagcg tcaaaattca aacgataccg
     2521 gaactgctta tccagacacg aggtaatatg accgaagttt cacgaatgct gaactgtaat
     2581 cgcgccacgg taagaaaata tgctgaagat aaagagggca aagggcacgc cattgttgat
     2641 ggtgttctga ttgttcatcg tggatgggac aggggtaaag acagtgatgc gtgatattca
     2701 gcaggttctt gagcgctggg gcgcgtgggt ggctaataat catgaggatg tgacctggtc
     2761 gtccattgct gccggtttta agggattaat cccttcaaaa gtaaaatccc gcccgcaatg
     2821 ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc cgccttaacc ggaacaacaa
     2881 cgatctacat gacttgctgg ttgactacta cgtgtttggg atgacattca tgacgctggc
     2941 gcggaagcat ggatgctcag atggttatat aggaaagaag ctacaaaaag ccgagggggt
     3001 ggtggaagga atgttgatga tgttgggtgt ccggttagaa atggacaaat atattgaaca
     3061 tccctgatgg taaggggcga ctttctagaa aatattttac gtacgtaaaa aacgggatat
     3121 tctgttaaga gtggttgctt cgccacacag cttaaacccg cctccgggcg ggttttttat
     3181 gcctgaaaaa cggtacagga cgttaaacac gctggtggtc agatgagttt gcagatgtga
     3241 tgacatatgg ttattattct gcctccggcc ctttagctca gttggtcaga gcgagcgact
     3301 cataatcgcc aggtcgctgg ttcaagtcca gcaagggcca ccaaccacca ctagctcatc
     3361 cggatagagc atcaaccttc taagttgacg gtgcgaggtt cgagtcctcg gtggtgggcc
     3421 agcgccgact tagctcagca gg
//
LOCUS       DAJTNP010000210         3404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTNP010000210 DAJTNP010000000
VERSION     DAJTNP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..238
                     /locus_tag="OO160_004100"
     CDS             <1..238
                     /locus_tag="OO160_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5525985.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO160_004101"
     CDS             248..886
                     /locus_tag="OO160_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5525986.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO160_004102"
     CDS             889..2052
                     /locus_tag="OO160_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5525987.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3404
                     /locus_tag="OO160_004103"
     CDS             2136..>3404
                     /locus_tag="OO160_004103"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5525988.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSI"
BASE COUNT          758 a          803 c          982 g          861 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatt
//
LOCUS       DAJTNP010000211         3350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTNP010000211 DAJTNP010000000
VERSION     DAJTNP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(216..419)
                     /locus_tag="OO160_004104"
     CDS             complement(216..419)
                     /locus_tag="OO160_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525989.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(421..576)
                     /locus_tag="OO160_004105"
     CDS             complement(421..576)
                     /locus_tag="OO160_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5525990.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(779..1186)
                     /locus_tag="OO160_004106"
     CDS             complement(779..1186)
                     /locus_tag="OO160_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5525991.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1263..1490
                     /locus_tag="OO160_004107"
     CDS             1263..1490
                     /locus_tag="OO160_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5525992.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1474..1896
                     /locus_tag="OO160_004108"
     CDS             1474..1896
                     /locus_tag="OO160_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5525993.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1974..2751
                     /locus_tag="OO160_004109"
                     /pseudo
     CDS             1974..2751
                     /locus_tag="OO160_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2758..3350
                     /locus_tag="OO160_004110"
                     /pseudo
     CDS             2758..3350
                     /locus_tag="OO160_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          860 a          744 c          900 g          846 t
ORIGIN      
        1 acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc ccccatggag
       61 atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc ggtgatattt
      121 atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat cgcgcgtctg
      181 tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag acccggtgaa
      241 tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc gccagattcc
      301 agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc cagttgctgg
      361 aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt ctcgttcatt
      421 ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt taaacgtttt
      481 gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca cgccgtacac
      541 cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc cccttaacgc
      601 cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat aaaatttaga
      661 ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt ttgatgtaaa
      721 aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa aaaggggatt
      781 atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg ttgaaatttt
      841 caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc agtgcgtcga
      901 acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta tccgggatcg
      961 gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag cactgaagcg
     1021 cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt tcccattgag
     1081 aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac agttttaatt
     1141 ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta gggaattcta
     1201 atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct aaggagctcg
     1261 atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt gccaatgccg
     1321 caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa ggtcgcgcga
     1381 tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt tatgacgaat
     1441 atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga gcacatcgaa
     1501 tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa cgcaattact
     1561 gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa ggactggaac
     1621 aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca aagggaaaaa
     1681 attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg tcaccgactc
     1741 tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc gttgagtacg
     1801 gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca tttcagcggt
     1861 ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt gccggattgt
     1921 tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa caaatggctt
     1981 caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc aggcttgctg
     2041 agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc tgggcatggg
     2101 cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt acaaaaagtg
     2161 caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag gttggatggc
     2221 tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac ggaaaaagct
     2281 ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg aaacgaaaag
     2341 gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa gaggaagagg
     2401 aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     2461 tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg catgagtggg
     2521 tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat acgggaactg
     2581 gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag catcgccaat
     2641 gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct ccgacaggtt
     2701 aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta acggaccatg
     2761 aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc gcaacatgta
     2821 accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga aggccagaaa
     2881 cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat tctgaatcgc
     2941 tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt gcagaacgac
     3001 ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat gaccgggtgt
     3061 acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc ggcagctatc
     3121 gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc tgatgttatg
     3181 agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt gcgggagctg
     3241 tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac gaaaaacgag
     3301 caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
//
LOCUS       DAJTNP010000212         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTNP010000212 DAJTNP010000000
VERSION     DAJTNP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            419..1528
                     /gene="fepE"
                     /locus_tag="OO160_004111"
     CDS             419..1528
                     /gene="fepE"
                     /locus_tag="OO160_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5525994.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2553..2747)
                     /locus_tag="OO160_004112"
                     /pseudo
     CDS             complement(2553..2747)
                     /locus_tag="OO160_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          802 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaac attatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
      121 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
      181 gtcattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
      241 ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg cgttcagcgc
      301 gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc tgctgaatgg tttttaggtt
      361 ttttcctaac atatgataga ataaaaaaac catatgttaa tatattgtgt ttttttcaat
      421 gaaaatctca aaggtctctg acgaacattt ttctaattac aacatgttgc cgaatggtaa
      481 tgaagaaatt gacctttttg gcttgattag cgttttatgg gcagcaaaaa taaaaattgt
      541 tggggtgatt ctattttttg cttgtattgg ctttcttgtt tcttttgttt tgccacaaaa
      601 atggactagt tctgctgtgg ttactcctgc tgagtctgtt caatggagtg aattaaataa
      661 ggagctttcc aagcttcatg ttctgggagt ggattttgat atcaatcgtg atagcgcatt
      721 tgctctgttt attaagaaat ttcagtccgt taattcgttg aatgagtata tgagatcatc
      781 tccttatgtt atggagatta ttaaaaagaa taacattagc gctctcgaac tgcatcgtgc
      841 aattgttgag ttgagtaaaa atatgaagtc gattgatgat aatgccagta agaaaaacga
      901 caagtcatca ttgtatgtat catggactct gagttttact gctccaacaa gtaaagaagc
      961 tcacgatgtg ttgtctgggt atattaatta tgtttcttcc cttgttgtaa gggatttgat
     1021 ggaagatata agaaataaac tagaagttaa aactaatgtt gaaaaagaaa ttcttgcact
     1081 ggatgagata aaaattagaa accagctgaa tgcagatatt cgacgcctca attattcact
     1141 ggaggttgct aatgcggctg gaataaaaaa acctgtatac agcaatggtc agattatgaa
     1201 ggatgaccca gattttcctg tggctctcgg ttctgatggt atagcaacta aattgaacat
     1261 caaaaaatca atcaaggatg tttcggaatt gagtggggag ttgcgaaatc gtcaatatgt
     1321 tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat tttatgccat ttcagtatca
     1381 attgagccca acgttaccgg tcagaaagga tgggccaagt aatgccataa ttgtaattct
     1441 ttcttctgtc attggaggga tgcttgcatg tggctatata cttttacgtc atgccatgga
     1501 ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga tgcatcattt tataagcaac
     1561 ttttataaaa agttcaaagg atcttcttga gatcattccc ttacagccgt agtcttctac
     1621 tctagaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga
     1681 acttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttctagtg
     1741 tagcctcagt aaggctgcca cttcaagaac tctcaataca tctctcgcac atcctgttta
     1801 ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacgaac gcggccataa
     1981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     2041 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccac cggcgcggcc
     2161 ttttttcggg gcctcttttt tgctcacatg ttttttctgg tgttatcccc tgattctgtg
     2221 gataaccgta tatccgtctt tgcgtgagca gacaccgctc gccgcagccg aacgaccgag
     2281 cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga agctgcattt tctccttacg
     2341 tatctgtgca gcatttcaca cctcatatgt cgctgttctc tcaccatctg ctctgccgcc
     2401 tcataattaa gccagtatac actccgttat cgctacgtga ctgggtcatg gctgcgcccc
     2461 gacacccgct aaaaccccct gacgcgccct gacgggcttg tctgctgacg aaaacgccca
     2521 cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc ctgtttcact ttcggcctca
     2581 aagcgaatct ggatgctgtt ctggagttct tctgcgagtt cgtgcagtcg tttgcacatg
     2641 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     2701 ttgatacatc gcgccatatt cagcgcagtt tgttgttatt tgttcacctt ccgttgttct
     2761 ccgtcagttc cgggtgtcgg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAJTNP010000213         3176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTNP010000213 DAJTNP010000000
VERSION     DAJTNP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(183..1892)
                     /locus_tag="OO160_004113"
     CDS             complement(183..1892)
                     /locus_tag="OO160_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5525995.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2324..3043)
                     /locus_tag="OO160_004114"
     CDS             complement(2324..3043)
                     /locus_tag="OO160_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5525996.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          969 a          607 c          534 g         1066 t
ORIGIN      
        1 tttctgctca ttattagctc cttctgatgc cattctattt caggaaggag tgtccgttaa
       61 actcaggcta cctcaggttg ccaattgagg tggttgcctg aaagttggca gttacccggt
      121 cactacacct tgttggagtg aacatatcaa aaacaggtaa ttacacaatt taaattacag
      181 tctcataaaa aatgattaaa tctatttatt atttcaataa acgttttttt catcttcttt
      241 gaaaattgtt ctgctatagt atccggttcc agattgttct cttttattat tctacaatac
      301 tctaatattg tttctttgtc tctatttact acagatacaa atacaccagg aataccatct
      361 ttattagttg cggacagcat ttcgaccaat cgtcttgtgg taaaactata gttttttaga
      421 atattacaaa aggcacgtat tgtttcggta tggccattat aaaatgccat aaaaagtcct
      481 gatgtaccgt tgggattttt tatccataat aattcatcta tatattcctg gtgagggggg
      541 agtattttta gtatatctcc atacgccata atggcctttg tgtgcccatt ttgaagtgcc
      601 ataaacaacc catgctttac tttcaataat tctttaattt gttcactact aagcattttc
      661 ttattaagca gacttccgaa catacgaata ctatctgcat gtccattctg caatgcaaaa
      721 aataacccag gtaaatcatg gctgtctttc gcagctaata gatataatct cttatcggaa
      781 gttaacgggg ttttaaagac tatttcccca taggctttta ttgtgtctac atgtccgttc
      841 ataaaagaca tgcataaacc cggcacattg tttgccccta ccgcagcaag caatgtaagt
      901 attgtttctt gactaagatt gcacatattt aatatcttac catatacttg aataatgtct
      961 gaatatccgt tttgaagtgc tatataaaat gcaggagtac catcagagct tctcgtggcc
     1021 agaaggttca ccagtttaga ttgagaaatt tcaccatttt tgtatttttc catcagtata
     1081 aataatactt ctgtcagatt ctttgttatc gcatgatata taactctgga attgatcaat
     1141 tcatctggta aaagtttcac tgtgtttgtt agaggggaat tatgcctatc ttcaaatatt
     1201 acctgcccgg gttgctccag accataaatt ttccaataaa aatcatcaaa aaaatcctta
     1261 gcggataatt gactcagttc gaaatggcta tcacaagtaa atacagtacg tttatgagta
     1321 acagttcgat taggatcgaa aaactgaaca acccattttt cactttcttg tacgcgcttt
     1381 attttcaacc ctaacgccat ttggtggttt aatgtgcatg ctgataatgt tctgacaaag
     1441 tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa ctgttcccca ctttttattt
     1501 tccacaaaat aaatataagc tggtgatttt gaaaaatctt ccgttttttc caaacataca
     1561 ttttttgcaa tagcatcagg ggtgctaaga agctgatagt ttaccttccc atttgtttcc
     1621 ataaaattca tttcccaata ggaagctagg tgacggcaaa ctatttgtct tccatatata
     1681 tcttttgcca ctccatttat atttacctcc acatttctct tttctgaaaa ataaggtgct
     1741 gttttattct taaactgaat tagttcactg gagtttgaga ccctcataat agaacttgtt
     1801 attgttctat tatctctgtt aacagacata tctgttgata acgaattttt tgttgaaaat
     1861 atactagatg aaaatgtttt atttaacggc atagaagaca accatagaga tgttataata
     1921 ctattagatt gcagttacta atagtaaaag cctccttaca aggaggtagc ttattcagaa
     1981 tgctcctata agatagcgag ctcgtcaacg atacagatga tctgattata ttgtgcaaaa
     2041 ggagaacagg aaaataggca ctggaaaaga tgaaggcctg atgggagcat taactaatgc
     2101 aaaatccgac aggttaatat cccgaaaagc aatcaaacga ctcaaacagg agatgcacgc
     2161 tgaaatggtg gacaactgtt cggatttcag atgagtagct cagcagttta tgagccgaat
     2221 gctctgtcac gggcaggaat caggctgcat acgcagtgcc aaatctaatc gtagttattc
     2281 taaccagttt tcaagctaca aggtggtgta gctggcatct tctctactct atcatcaaac
     2341 gataaaatgg ttcctctcgt aacatttgct cttgcctttc acttccatct cgatcgcttc
     2401 gtagttcatt acgatagctg acatatttcc agtcttcagg tctaacatct gacgccggat
     2461 actccccggg agcgattttg cttcttaata gattagactc gagacacatt ataaattgtc
     2521 gtattttatg aagtaataaa gcactatatt gcgagcattc ctgatctgat tttacatata
     2581 gcgtaaactg agctcctatc cccacacgag attgctgaga aactcgattc atatcagtta
     2641 tcttccattt atctatagga ctgtcctctg aaaataataa accagacaaa atttggaatg
     2701 ccagaggaac ttgctcccta gctatactaa tatgaaactt gtccccaaca aaatccccct
     2761 tactctgagg attttcacga catgcatgaa taaacacatc tccactatgg ttcacaatct
     2821 ggaatccttg gtacatgaca gaaaaagatt gacatatatc agagtaagtt ggcacgttcg
     2881 atgctacttt gaaatagtac gctgggtatt gccgtatttg gttgtacaat atattgaagt
     2941 tatttttcaa tctttcattc gcagaaagca tctgcggtat ttcagacctc acaacattca
     3001 aagaatctaa atttagtttt agacagggct tttttatggg catagaaaac gtcctctata
     3061 aaatagatat attatcttat attgattgtt tatcatcttt tataaaacga tacattatat
     3121 gtaatgtatg taagcgtaag ggtcctctcc gaacatggtg ggaaggctta gaacgc
//
LOCUS       DAJTNP010000214         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTNP010000214 DAJTNP010000000
VERSION     DAJTNP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO160_004115"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO160_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5525997.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO160_004116"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO160_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO160_004117"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO160_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5525998.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO160_004118"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO160_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5525999.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO160_004119"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO160_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTNP010000215         3076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTNP010000215 DAJTNP010000000
VERSION     DAJTNP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..250
                     /gene="pgrR"
                     /locus_tag="OO160_004120"
     CDS             <1..250
                     /gene="pgrR"
                     /locus_tag="OO160_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5526000.1"
                     /translation="RMEGQLLLNTIDLIIDVAIDGHGLAYLPYDQVERAIKEKKLIRV
                     LDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            587..2200
                     /gene="mppA"
                     /locus_tag="OO160_004121"
     CDS             587..2200
                     /gene="mppA"
                     /locus_tag="OO160_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5526001.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2251..>3076)
                     /gene="ynaI"
                     /locus_tag="OO160_004122"
     CDS             complement(2251..>3076)
                     /gene="ynaI"
                     /locus_tag="OO160_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5526002.1"
                     /translation="LKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDIT
                     SARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGI
                     MLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENP
                     GRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLN
                     IMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          867 a          673 c          721 g          815 t
ORIGIN      
        1 tcgcatggaa ggtcagcttt tactgaatac gatagacctg atcattgatg ttgcaattga
       61 tgggcatgga ttggcgtatc taccttatga tcaggttgag cgggctatta aagaaaaaaa
      121 actgatacgt gttttggata aattcactcc agatttaccc ggttatcacc tgtactatcc
      181 acaccgtcga catgctggct cggcattctc attatttata gataggctga agtataaagg
      241 tgctgtttag cactacttgc tgatacatta atttaattct tctcttaacg tattctcagt
      301 tcctttcaac gttttggtta ttttttattc ttcgtacaat ggcgacagat gctgattatg
      361 ataccgaaaa ctggtttgaa cgtgcgaagc ccgaacgtag tgttcggtgt tctatgtgct
      421 ttgccacatt ttggcgtcta ttatttacac taaatctgat ttgatatatt gatacttaaa
      481 acatttgatg cttcctttgt cacttttttg atggaagttg tttgcatttc tttaaggcga
      541 aacaaataat tacgcatcaa ttttaatgtc ggttagaggg aaacttatga agcactctgt
      601 ttcagttacg tgttgtgcgc tgttggtcag cagcatttct ctttcgtatg ctgcagaagt
      661 tccgagcggc acagtactgg cagagaagca ggagctggtg cgccacatta aagatgagcc
      721 tgcgtcgctg gatcccgcta aagccgtggg cctgccagag attcaggtca ttcgcgatct
      781 gtttgaaggt ctggtgaatc agaacgaaaa aggggagatt gtccccggcg tcgcgactca
      841 atggaaaagt aatgacaacc gtatctggac ttttaccctg cgcgataacg caaaatgggc
      901 ggatggcaca ccggtaacgg cgcaagattt tgtctacagc tggcaacgtc tggtggaccc
      961 aaaaacattg tcgccatttg catggtttgc cgcgctggcg ggaatcaaca acgcacaggc
     1021 gattattgat ggtaaagcta cgcctgatca gcttggcgtc accgcagttg atgcccatac
     1081 tttgaaaatt cagcttgata aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc
     1141 cttcttcccg gtgcaaaaag ccaacgtaga aagcggtaaa gagtggacga aacccggaaa
     1201 tctgatcggc aatggcgctt atgttcttaa agagcgcgta gtcaatgaaa aactggtcgt
     1261 ggtaccgaat acccattatt gggataacgc caaaacggtg ctgcaaaaag tgaccttcct
     1321 gccaattaat caggaatccg cagccactaa gcgttacctc gcgggggata ttgatatcac
     1381 cgaatccttc ccgaaaaata tgtatcagaa gctgttgaag gatattccgg ggcaggttta
     1441 tacgccaccg cagctcggga cctattatta tgcctttaac acgcaaaaag ggccgacggc
     1501 agatcagcgc gttcgcctgg cattaagtat gacgatagat cgccgcctga tgaccgaaaa
     1561 agtgttgggg acgggcgaaa agccagcgtg gcattttata ccagatgtta ccgccggatt
     1621 tacgccggaa ccttcgccgt ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa
     1681 aactttgttg agcgcagctg gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta
     1741 taacacttca gaaaaccatc aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa
     1801 ccttggcgta gatgttaaat tgcaaaatca ggaatggaaa acctatatcg atagccgtaa
     1861 caccggcaat tttgatgtta tccgcgcctc atgggtgggg gattataatg aaccctccac
     1921 tttcctgaca ttattaacgt caatgcattc aggaaatatt tcacgcttta acaatccggc
     1981 atacgacaaa gttctggccc aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga
     2041 ttacaacgcg gcagaaaaaa tcctcatgga gcaagcaccg attgcaccaa tttatcaata
     2101 taccaatgga cgattaatca aaccgtggct gaaaggttat cccattaata accctgaaga
     2161 tgtagcgtac agtcggacta tgtatattgt gaagcattga tgtgatggga actggcgtta
     2221 cccttgtgga taacgccagt gatgtctgat ttagcgcccc tgttccggcg gtgtaatgtt
     2281 atccatatac agcgtctggc tggggaaggc aaagtccgcg ccgtgtgact gtacaatatc
     2341 gataatcttc aaataaacgt cttgctgtgc agcaagccac tcagcccata ccgtggtttt
     2401 ggtaaagcaa taaaccataa tattcaatga cgagtcagca aactggttga aataaaccag
     2461 taaggtttgt cgctggtcga tggccgggtg atttttcagc atctcacgta cggcttcgac
     2521 aataacgccc acttttgccg catcctcata acgtaaacca atggtcgtgg taatgcggcg
     2581 gttggtcatt cgtcctgggt tttctacgct gatcgacgaa aacagcgagt tcggtacgta
     2641 caatggacga ttatcaaagg tcgtaatttt ggtaattcgc cagccaattt ccgctactgt
     2701 accttcgata tttctgtccg gtgaacggat ccagtcgcca atactgaaag gacggtcgaa
     2761 atagagcatg atcccggaaa agaaattact cagaatatct ttaccggcca taccgacagc
     2821 cagaccacca ataccaccaa aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc
     2881 ataaagcaga acaagcacta caataatggt gattttgata atgcgcgaca taatccgcgc
     2941 actggtgata tcgcgacctt ttttaatctg ctgtttttca aactgattaa tcagcagaaa
     3001 tagcttaatc gtcagaataa ccgcaatcag ggacgtacag ataaaatcga taacgcctgg
     3061 ggtgataaat ttgagt
//
LOCUS       DAJTNP010000216         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTNP010000216 DAJTNP010000000
VERSION     DAJTNP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..498
                     /locus_tag="OO160_004123"
     CDS             <1..498
                     /locus_tag="OO160_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5526003.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO160_004124"
     CDS             578..1273
                     /locus_tag="OO160_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5526004.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="OO160_004125"
     CDS             1302..>3069
                     /locus_tag="OO160_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5526005.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAJTNP010000217         3002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTNP010000217 DAJTNP010000000
VERSION     DAJTNP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..137
                     /locus_tag="OO160_004126"
     CDS             <1..137
                     /locus_tag="OO160_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526006.1"
                     /translation="GLIVPALISMKDTVAVISGFALACLTPPCIYAIYKGLSFSKDKR
                     "
     gene            165..959
                     /locus_tag="OO160_004127"
     CDS             165..959
                     /locus_tag="OO160_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5526007.1"
                     /translation="MLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEVVGVGRYWTGWN
                     TEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTVFQTYRKGVDDI
                     TDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTPIGIQVMSLSYV
                     GKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQADAIRTKAQAE
                     ADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1003..1752
                     /locus_tag="OO160_004128"
     CDS             1003..1752
                     /locus_tag="OO160_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5526008.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1749..2318
                     /locus_tag="OO160_004129"
     CDS             1749..2318
                     /locus_tag="OO160_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526009.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2442..2684
                     /locus_tag="OO160_004130"
     CDS             2442..2684
                     /locus_tag="OO160_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5526010.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2688..2834
                     /locus_tag="OO160_004131"
     CDS             2688..2834
                     /locus_tag="OO160_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526011.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2843..>3002
                     /gene="xisR"
                     /locus_tag="OO160_004132"
     CDS             2843..>3002
                     /gene="xisR"
                     /locus_tag="OO160_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5526012.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          809 a          725 c          790 g          678 t
ORIGIN      
        1 acggattaat cgttccggcc ctcatatcaa tgaaggatac ggtagcagtg atttctggct
       61 ttgctctggc gtgtctgacc ccgccctgca tttatgccat ttataagggt ctttcttttt
      121 ctaaggataa aagatgaaaa aagttattcc tgctttagcc cgttctgctg accaccggac
      181 ttgtcggttg cgatcgcgtt gagcctggta atgttggcat caaggtaaac aaactgggcg
      241 acgataaagg cgtcggagaa gtggtcggtg ttgggcgcta ctggacggga tggaacactg
      301 aggtttacat cttcccgacc ttcaaacaaa tgaagaccta cgatgagcca ttcagcttcc
      361 agatgagtga cggtacaacc atcggctatc acatcggcgt ggcctacaaa gttgatccat
      421 ccaaagttac cacagtcttt cagacctacc gcaaaggcgt ggacgacatt accgacaccg
      481 acctgcgcca gaagatagcc gacgcactca accgactggc cagcaaaatg accaccgaca
      541 aatttatcga cggcggcaag tctgagctgc tggatgcagc tcttaaagac attcaggaag
      601 aaatgacgcc catcggtatt caggtaatga gcctctcata tgtgggtaag ccggagtacc
      661 cgcctactgt tatcgacagc attaatgcca aagtcacggc gaaccagaaa accctgcaat
      721 gcgagcagga agtaaaacag cgcgaagcgg aagccaacat gttgcgcgcg gaagctgccg
      781 gacaggcaga tgcgattcgc acaaaagccc aggccgaagc cgatgctatt cgtttacgcg
      841 gtgaagctct gcgccagaac ccaaacgtta tggagctgga agccatcaac aagtggaacg
      901 gtacactgcc gcaatacatg accagcggtg ccaacacacc atttattcag gttaagtaat
      961 acatatgccc ggtattacac gccggggtgt ctggagataa aaatgaatac tgtaaccatc
     1021 aacaacaaac agtttccggt aatcgaatat cgcggtcagc gtgttgtgac gctggcgatg
     1081 attgatgaag tccaccagcg accggaagga accgcgcgtg cagcattcaa ccgaaaccgt
     1141 gagcatttca tcagcggtgt ggattatgcc gaattaggtg cggacgtaat acgtacggac
     1201 ctcccggaag gaacattctc taaatttgca ccatcgggga ttgtactttt tgaatcaggt
     1261 tacctgatgt tgacgaagcc attcaatgac gcccttgcct ggcaggttca gcgcgaactg
     1321 gttaacagct acttccgcac tcgcacaccg ctaacggaaa tcgagatgat cgctgcgata
     1381 gccgccgacg ctgttcgtca acagaagcgc ctgaatcatg tggaagaaca gctcgaaaca
     1441 gtcacagaag cggtggaaac catcaaacgc ggaaatatgc gcgccggata tgtcggttac
     1501 cgccaggtgg tagccaaaag tggaatgagt gacaccaagt gtcggaatct ggtgaacgca
     1561 tatcaaattc ctacagacac ccacgaattc atgactccgg acggtctgtt gtctcgcagg
     1621 gctatcgtcg agcttgagcc atttatggcg gcgttccacc aaatgatgtc agaggctgac
     1681 ccacgcggca cacgctggta tcacccgaag atggggcttt tccaggtgat cggatgggag
     1741 gataaagcat gatcatccag tcaaaactta ttcgcgccgc tctggtgtgc gctgctaaaa
     1801 acgacgttcg ttattacctg aacggtcttc acatcacgcc gaaacatatt gaggcaacca
     1861 atggttccgt ggcactgcgc atggctcacg gcatccggac gaagaaaaac atcattgtcc
     1921 agttcgaagg gggcgtcccg gccaaagccg aaacgacaga gctgattttt agtaaagagc
     1981 cgatcgctgt tcatcgcgac cagtttcagc gacgactgtc cattaccggc attaaactgg
     2041 tggacggttg ttttccggat ttggatcgca tcattccgaa aaagtttgac cgctgtactc
     2101 acccggtgct ccaggcgggt tacctgagtt atccagagaa gatgtttggt cgtgagcgta
     2161 aatttattcc cgtccagtta cgtccctccg gtgacgggca agcggtcaga attcagtttg
     2221 attccatcat caactcaatg tatggcaatc ctgaatttgt tgtgatgcct tgtcgtgatc
     2281 atggcgattt caacgtggct caggagcatc cggaatgaaa atcgaatacc aggacgccgc
     2341 aggaggtgaa tcaatgagct ggcctgatgc aatcgtaact ctggggatgg tattcgcagt
     2401 agcgtttgtt gtgtactcga tttgtcgatg gggataacca catgttcgct ttgattcaac
     2461 gcgggcagat atacacagac agagccggat acccagtaga aattactcgc agtactgagc
     2521 actcagtgtt ctttcggagg atggacggac gtaccgggcg agtacgcatc ggtgaattca
     2581 gtagcctgtt cgagcatatt gaccacctgg aatatcacaa gattctggct gaaacagaac
     2641 aagaaaagca cctgaagaaa ttacgcgcaa tgcaaaggaa gtaaaaaatg aataaagcgt
     2701 ttgagctatg ggtccaccag cgttacggca accactacga cctgacgcga gatgttgacg
     2761 gtttctactg tcgtgaaatt gtgaaacgaa tgtttgaggt ctggtgccac tgccgtgggc
     2821 tgagtgttgt gtgaggtaat gcatgggcaa tgtgattcaa ctggctccca atgaatgggt
     2881 ttgtgaaagc gttctaatcg caattaccgg gctcaaacca ggcacaattc ttcggacccg
     2941 gaaagaatgc tggatggttg gaagagagta tattcacgta tcaccagacg gtaatccaaa
     3001 gc
//
LOCUS       DAJTNP010000218         2941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTNP010000218 DAJTNP010000000
VERSION     DAJTNP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            131..1999
                     /gene="kup"
                     /locus_tag="OO160_004133"
     CDS             131..1999
                     /gene="kup"
                     /locus_tag="OO160_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5526013.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2166..2585
                     /gene="rbsD"
                     /locus_tag="OO160_004134"
     CDS             2166..2585
                     /gene="rbsD"
                     /locus_tag="OO160_004134"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5526014.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2593..>2941
                     /locus_tag="OO160_004135"
     CDS             2593..>2941
                     /locus_tag="OO160_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5526015.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          656 a          700 c          772 g          813 t
ORIGIN      
        1 tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc cgttaatcgt
       61 gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata tttcaacgaa
      121 agactagtct atgagcactg ataataagca atcattgccc gcgattaccc tcgcggcgat
      181 tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg aatgtttgtc
      241 cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat cgctgatctt
      301 ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc gggcagataa
      361 cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg
      421 aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct atggtgaagt
      481 cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa tcgtcgcccc
      541 gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat tatttatgat
      601 tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt
      661 tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag tgctgcatgc
      721 gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt cttttattgc
      781 attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg atatggggca
      841 ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt ccttgaccct
      901 taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta agaacccgtt
      961 cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg cactggcgac
     1021 ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc aggcggtacg
     1081 tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt cagggcaaat
     1141 ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt
     1201 tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa ccatggtact
     1261 gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata agtattttgt
     1321 tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg ctaacctcga
     1381 taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt ttatcgtgat
     1441 gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac atggtaactc
     1501 tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc ccgggaccgc
     1561 ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata accttaaaca
     1621 taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag acgcgccata
     1681 tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct ggcgcgtggt
     1741 ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc gctgcggtct
     1801 ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg agtcgttgat
     1861 cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg
     1921 taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta tcgaactggg
     1981 tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc gtttttgttt
     2041 tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta acgtcacgat
     2101 ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg ctgatggaga
     2161 aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tcccgtctgg
     2221 gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc
     2281 gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg ggcgtcgtca
     2341 caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa cataatccgc
     2401 aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaatacca
     2461 ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg
     2521 taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt
     2581 tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc cttcccgggc
     2641 gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat ggcgctggtg
     2701 ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat ctatactcgc
     2761 gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc gaaatcttcc
     2821 caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca gttgaccatt
     2881 gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa
     2941 a
//
LOCUS       DAJTNP010000219         2931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTNP010000219 DAJTNP010000000
VERSION     DAJTNP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..2743
                     /locus_tag="OO160_004136"
     rRNA            <1..2743
                     /locus_tag="OO160_004136"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2839..>2931
                     /gene="rrf"
                     /locus_tag="OO160_004137"
     rRNA            2839..>2931
                     /gene="rrf"
                     /locus_tag="OO160_004137"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          769 a          650 c          919 g          593 t
ORIGIN      
        1 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
       61 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
      121 agtgtgtgtg ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac
      181 acaaaaatgc acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
      241 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
      301 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
      361 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
      421 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
      481 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
      541 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
      601 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
      661 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
      721 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
      781 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
      841 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
      901 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
      961 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
     1021 gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta
     1081 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     1141 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     1201 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     1261 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     1321 ggttttccag gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc
     1381 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     1441 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     1501 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg
     1561 aagcgacttg ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     1621 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     1681 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
     1741 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     1801 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     1861 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     1921 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     1981 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     2041 tggacccgtg atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     2101 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     2161 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     2221 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     2281 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     2341 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     2401 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     2461 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     2521 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     2581 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
     2641 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     2701 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt
     2761 ttggcggatt gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     2821 ctgataaaac agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg
     2881 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca t
//
LOCUS       DAJTNP010000220         2922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTNP010000220 DAJTNP010000000
VERSION     DAJTNP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO160_004138"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO160_004138"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5526016.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO160_004139"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO160_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5526017.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="OO160_004140"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="OO160_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5526018.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2922
                     /locus_tag="OO160_004141"
     CDS             2753..>2922
                     /locus_tag="OO160_004141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5526019.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASG"
BASE COUNT          657 a          798 c          802 g          665 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc gg
//
LOCUS       DAJTNP010000221         2921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTNP010000221 DAJTNP010000000
VERSION     DAJTNP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..757
                     /locus_tag="OO160_004142"
     CDS             <1..757
                     /locus_tag="OO160_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5526020.1"
                     /translation="TPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKN
                     KRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINP
                     EESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDAN
                     TAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAP
                     NHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1106..2278
                     /locus_tag="OO160_004143"
     CDS             1106..2278
                     /locus_tag="OO160_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5526021.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2332..>2921)
                     /locus_tag="OO160_004144"
     CDS             complement(2332..>2921)
                     /locus_tag="OO160_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526022.1"
                     /translation="GGADLAIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGG
                     GKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVT
                     RSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSC
                     DVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          908 a          607 c          580 g          826 t
ORIGIN      
        1 cacgccaaaa ccagcgtccg ttgcaacgaa ggctacgcat ctttcattta taaaggccct
       61 actaagagcc gcagagcgtg aatggaaaat gctggataag gcaccaatta ttaaagtgcc
      121 tcaaccaaag aataaacgga tccgctggct ggagccccat gaagcacaaa ggctgattga
      181 tgaatgtccg gagccattaa agtctgttgt tgaatttgca ctggcaacag gcttaagacg
      241 ctcgaacatc atcaaccttg aatggcaaca aatagatatg cagcgccggg tggcatggat
      301 aaacccggaa gagagtaaat caaaccgcgc aattggcgtt gcgctgaatg atactgcatg
      361 tcgcgtattg aaaaaacaaa tcgggaatca tcaccgttgg gtatttgtgt acaaggaaag
      421 ctgtaccaaa ccagacggaa cgaaagcgcc aacggtcagg aagatgcggt atgacgcaaa
      481 cacagcctgg aaagcggcgc tgagacgagc aggtattgat gatttcagat ttcacgactt
      541 gagacacacc tgggcaagtt ggctggttca agccggagtc ccgttgtcag tgttacagga
      601 aatgggaggc tgggagtcta tcgaaatggt tcgtcgatat gctcaccttg cacctaatca
      661 ccttaccgaa cacgcacggc aaatagactc gatcctgaac ccatcggtcc caaattcgtc
      721 ccagtcaaaa aataaggaag gtactaatga tgtgtaactt attgatttaa atggtgccga
      781 taataggagt cgaacctacg accttcgcat tacgaatctg tagcaccaat cataactatc
      841 tgttttagca agcattaacc gcattcacta agctatagtt gatgacacaa acagaaagtt
      901 gatgcatgat gttgccatat atatgtcaca aatacggcac aacgatcttc aaacatatag
      961 ccacacattc acagaagagc acaaagcctt gcaatccagt gcaaagcttg gtgtgtctcc
     1021 gttttgtttc atcagttaca cataactata tgatcattaa ctaaaagtca gtatacttgg
     1081 agaaatttta attgcgtaat agagcatgca tatgattgaa attaacagtt tattattaat
     1141 aacatccgtg atattgatgt cgttattagc tgtggggtta tttgataaaa tttccccaat
     1201 aaatcttgtt gagcacggca ggaataatca aatagatgga atgcgggggt tcttagcaat
     1261 tttcgtgctt attcatcacg cagcaatttg gaatggatac ttgtcatctg gagtatggga
     1321 agcaccttca tcaaatctgt tagcaaattt aggccaagtt ggtgtgtcat tcttttttat
     1381 gattactggt tatctgttct tttcaaagat tatctctgga gatcaggact ggacaaggct
     1441 ttatgtatca agattactac gattaacccc aatgttcata gttagcctat gtctcatttt
     1501 tatcattgta ggttttaagt ctggatggag aatgcaggta tccacagaag agctttttgt
     1561 gtcaataatg aagtggttgc cattcactgc gctaggtatg ccgaacatta atgacgtaaa
     1621 agattcattt actatcaatg ccgctgtaac atggacactt gtatatgaat ggttctttta
     1681 tttttctctt ccggtaattt ccgcgctcat aaaaagaaaa gtcagtattt acatggttat
     1741 gattagcgca atatcgctat ttgttttcat tttatttttc agtaaaatac acatagcatc
     1801 atttcttttt gggctcctgg catttttgct aaataaatca aagatagtca atggaattgc
     1861 caaagcaaaa gtaacaccaa ttataataac tgcgataatg gtttttgaaa tgacgtattt
     1921 caaaacaact tacgcaccgc taccactgat tctttgtgga ataacattta tcattatcgc
     1981 atcaggttgt gatttgtatg gaatattaag attaaatata accagaaagc taggagaaac
     2041 tacctacagc gtttaccttc tgcatgggat attcctttat tgcctaatga cgtggattat
     2101 tcctaataat tacactgaaa atacatttat catactggta agcacaactg catttcttat
     2161 tacgttcaca tcatgcctaa cttttaaatt aatagagacg ccattcatca aattaacaaa
     2221 acaaaccaca actttagtaa aagaattaat acccacatta acaaacaaca atcaataaac
     2281 aataaacata agccgcccta catatatgtc caaatggggc ggcgaatgta tttatattag
     2341 tctggtaatg ctaaatgcaa acgaactgga gattcctgcc ccgctaagcg tattgcgcag
     2401 ggtcacccta aatgtatcta cgccaatata agtaacatca caactaactg aatcaggcgc
     2461 agatataggt gttatctgca caatataacc gctagcaact gctgcgagcg gatgagaaaa
     2521 gctaacatca tacacacctg tagaaaccac gttaaccgat gaaacaccag tagcaacgtc
     2581 gatactacca ttggcgttaa tttttcccga gcgtgtgact gacaatgcag aagccgatgt
     2641 taccttactg gttgtttctg taaaaacaca cccaatgtca actacctcgc aattagctcc
     2701 tgttatccag tatggtcgtt ctgcacttgg ctgataatcc cctgcgctct ggaacgttgt
     2761 gccgttgagt atgactgtaa cttttccccc acctgagctt gttacttgaa tattcgtgtg
     2821 cgtgtatctt gcactgctca cgcgatgaaa atttccaccg tttataacca cagttacagg
     2881 gcgcgtccca gtattatcta tagcaaggtc tgcccccccg g
//
LOCUS       DAJTNP010000222         2919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTNP010000222 DAJTNP010000000
VERSION     DAJTNP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            174..1715
                     /gene="istA"
                     /locus_tag="OO160_004145"
     CDS             174..1715
                     /gene="istA"
                     /locus_tag="OO160_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCR5526023.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1730..2476
                     /gene="istB"
                     /locus_tag="OO160_004146"
     CDS             1730..2476
                     /gene="istB"
                     /locus_tag="OO160_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR5526024.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            2562..2919
                     /locus_tag="OO160_004147"
                     /pseudo
     CDS             2562..2919
                     /locus_tag="OO160_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          840 a          685 c          708 g          686 t
ORIGIN      
        1 gatcactcaa taacttccat cgagatcggg taataacatt tgaacagatc gctgaataac
       61 atcgatggag atcacttttg actcattttg ttattcagtg atctccatca atgttattgg
      121 aacttcacag gtgtgttgat ctgtatcttt tgccattccg gtaaaggata cctatgccaa
      181 cagttccaat ttctatgaga aaacttaaag aaattcttag gcttaaatac ggtgttggac
      241 tcagccatcg acaaattggt cgtagtcttg caatctcccc ttccgttgta tccagatatg
      301 ctaatcgggc ggctcaactt ggcataaagc agtggccctt acctacagga tgggatgata
      361 caaaactaaa acatgcgttc cttcagaccc aggttaagat gaagaagcac tctctgcctg
      421 actgggctac agtacaccgg gaactgcgta ataaatgcgt gacgctgcag ctactctggg
      481 aagaatactg tgagcgtaat ccaggcggtt tttacagcta taaccattac tgccggatgt
      541 accgtgaatg gctcaaaacc acttcaccat caatgcgtca ggtacataaa gctggcgaaa
      601 aacttttcgt tgattactgt ggacctaccg ttggcgttac cgaccctgag accggagaaa
      661 taagaactgc tcaggtcatc gtagctgttc tcggggcatc aagttacaca tgggcagagg
      721 ccacctggtc tcagcagctt gaagactggg tgatgagtca tgttcgctgc ttccagtggt
      781 tgggtggcgt tcctgaactt gttgttccgg acaatctgaa aagcgccaca tccagggcat
      841 gtaagtatga tcctgacgtt aaccctacct accagcagat gcttgagcat tataatgtcg
      901 cagttttgcc tgcgcggcca cgtaaaccga aagataaagc caaagctgaa gttggcgttc
      961 aggttgttga acgctggatc atggcccgaa tcaggcatga gatcttctac agccttgcat
     1021 cgcttaatca gcgcattcgg gagttgctgg aaagactgaa taacaaaata atgcagaagt
     1081 tgggttattc acgtgcagaa ctcttcatcc agcttgataa acccgcactg aagcctcttc
     1141 ctgaagccag ttacagttac accctggtga agaaagtcag agttcatgcc gattaccacg
     1201 tggaaatcga caaacattac tactcggttc catgttcgct gttaggccag caactggaag
     1261 catggatctc cggagaactg gtaagactct tcaatcaggg gcaggaggtt gctgtgcacc
     1321 cgcgcaagcg tacttatggc tacagtaccc gcaacgagca catgcctgaa gctcatcgac
     1381 agcatgccac ctggacgcca gagcgtcttc tggaatgggc ggggcacata ggcagtgaaa
     1441 ctcatagtta tgtgcttcat atactgaact ctcgtccaca tccggaacaa agctatcgct
     1501 tctgccttgg actcctgaac cttcataaaa aatacagtaa agccagactt aatgcagcat
     1561 gtgcaagagc tctgaaaaca aaggtatggc gtctgtcagg tattaaatcg atcctggaaa
     1621 aaggtctgga taaacaacct gttcaggatc caaaaccaga tctgttatcc acgatggaac
     1681 acgaaaacgt acgcggcagt gagtattacc actgatacgg gatccaatga tgaatcatct
     1741 ttacgaacaa ctgaccgcac ttaaactcac cggcttccgt gatgcgctta aaaagcaact
     1801 tgctcagccg ggcacatacc aggagctggg cttcgaagaa cgcctgtcat tactgacagc
     1861 agaagaacta acctgccgtg aaaacaggaa ggcagagcgt ctgatcaaac atgcacggtt
     1921 cagacttaat gctgagttat caaagctgga ttatcgtaac aatagagggc tggacagggc
     1981 cctcatccgt tcactcagtc agggaaactg gttaaccctg aaacaaaata ttttactgac
     2041 cggggccacc ggcagcggta aaacgttcct ggcatgtgca cttggtcata atgcctgccg
     2101 acagggatac aaggtctact attatcgcct taaagcgctg atggaacagt gctatcaggg
     2161 gcatgctgat ggaagataca gcaaactttt gaccaggctg aataatagcg atctgctgct
     2221 tctggatgac tgggggctgg aacctctctc atcagaacag cgtagcgacc tgctggaaat
     2281 agtggatctg atgtaccaac gaggctcaat catcgtagtg agccagttgc cggtggaaaa
     2341 ctggtacaaa atgatcggag actccacaca tgcggatgcc atcctagatc gactggttca
     2401 tggcagtatc aagatcgaac ttaaaggaga atcaatgcgg aaaatacaat ctccgttgac
     2461 cgaaggagat cagtgaaggt aatttaaaaa cggttctgtg aaagtgacac gaaccgatct
     2521 ccatcgatgt tactcaccga tctccttcac ggtaatacgc accaggctcc cgcacagtgg
     2581 ctgagctgga atctgaagtt atgcaactgc gtaaggcttt aaatgaagca cgacttgagc
     2641 gagatatatt aaaaaaagca acagcgtatt ttgcacagga gtcgctgaaa aatacgcgtt
     2701 agtcgaacaa tggcgacaac aattccctgt tgaagcgatg tgtcgggtac ctggtgtatc
     2761 caggagcggt tattacgacc gggtacagca tgccccctca gacagaaaac aaagtgatga
     2821 gcggctaaaa ctggagatta aggtggcaca tatccgcact cgcgaaacat atggaacccg
     2881 gcggctccag acggagctgg cagataacgg aattatcgt
//
LOCUS       DAJTNP010000223         2890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTNP010000223 DAJTNP010000000
VERSION     DAJTNP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..266
                     /locus_tag="OO160_004148"
     CDS             <1..266
                     /locus_tag="OO160_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5526025.1"
                     /translation="ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(282..545)
                     /locus_tag="OO160_004149"
                     /pseudo
     CDS             complement(282..545)
                     /locus_tag="OO160_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
     gene            738..1241
                     /locus_tag="OO160_004150"
     CDS             738..1241
                     /locus_tag="OO160_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5526026.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1287..1703)
                     /gene="rraB"
                     /locus_tag="OO160_004151"
     CDS             complement(1287..1703)
                     /gene="rraB"
                     /locus_tag="OO160_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5526027.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1865..2878
                     /gene="argF"
                     /locus_tag="OO160_004152"
     CDS             1865..2878
                     /gene="argF"
                     /locus_tag="OO160_004152"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5526028.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          809 a          671 c          681 g          729 t
ORIGIN      
        1 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
       61 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
      121 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
      181 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
      241 atctgaacat aaaacactat caataagttg gagtcattac cgattgcgaa atagaaaaaa
      301 acaaaaacaa gacaactaac aaaaacattc ccaccggtaa ttccataaga aaatcgttgt
      361 ccgttaactt ccatattagc ataaagataa cgcttacatt tcattaatgc ccatggtaaa
      421 tagataccta gcgttataat cgttaacaac acattcacca cacaaataag aaaatatctc
      481 tcacctgtac catgaaaaac aaaagaatgg gacggaacat ccatttcatt aataacttga
      541 gccataaaaa tcccctgaat taaaaaatcc attgatataa tttatagata cagacagtaa
      601 aatgttctgc cacgttacaa tactcttatc agtaataggc tggcaaaaac tttattgctt
      661 tataagacgc ttgcgcacgt aagaagagag tggtattaaa caaatgtata attactttat
      721 ttttattggg ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga
      781 cgctgcagga taatcctgct atcgccagcg tcatccgtca ggtttacgca gagtatggtc
      841 ttaccgccga taaaggctac accgttgccg atccgaatct cgacgaactg tatcaagtgt
      901 acagccagcc tggccatgct tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg
      961 gcgggattgc gccattaaca ggtagtgaag cggacatttg cgaactgcag aaaatgtact
     1021 ttcttcccgc tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc
     1081 aggcacgaga gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag
     1141 ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg
     1201 gccatgtcga ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg
     1261 tgctatcggg cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca
     1321 aaatcttcat cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg
     1381 tactcaacgt caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga
     1441 tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca
     1501 acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct
     1561 ttttccagag tttccagatc gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc
     1621 ggatcgctgc catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct
     1681 tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt
     1741 gtcacacacc actgacattg cctccacctt tgtgacaaag atttatgctt tagacttgca
     1801 aatgaataat aatacacata aattgagttt taattcattg aggcgttagc cacaggaggg
     1861 atctatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga
     1921 actcaacagc ctgctgcagt tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga
     1981 agaagccaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgactcgtac
     2041 ccgatgctct ttcgaagttg ccgcatatga ccagggggct cgcgttactt atcttggccc
     2101 aagcggtagt cagattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg
     2161 catgtatgac ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga
     2221 atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagttgct
     2281 ggcggatctc ctcaccatgc aggagcattt acccggtaaa gcattcaacg aaatgacgct
     2341 ggtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcggcgct
     2401 taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt
     2461 tacggaatgc cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt
     2521 agctaaagga gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga
     2581 agcaaaagaa aaatgggctg aacgcattgc attactgcgt gattatcagg tgaacagcaa
     2641 gatgatgcag ttgaccggta acccggaggt caaattcctc cattgcctgc ccgcgtttca
     2701 tgacgaccaa acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat
     2761 ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa
     2821 ccgcatgcat accatcaaag cggtgatggt cgcgacgctc agtaaattaa ataattaata
     2881 tcgctataaa
//
LOCUS       DAJTNP010000224         2715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTNP010000224 DAJTNP010000000
VERSION     DAJTNP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            54..2576
                     /locus_tag="OO160_004153"
     CDS             54..2576
                     /locus_tag="OO160_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5526029.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2587..>2715
                     /locus_tag="OO160_004154"
     CDS             2587..>2715
                     /locus_tag="OO160_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5526030.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          678 g          724 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      781 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      841 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      901 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtcagccg
      961 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt ggcgatttgg
     1021 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1081 tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt gaattgcgcg
     1141 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1201 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1261 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     1321 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaactat
     1381 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     1441 ataccctcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     1801 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     1861 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     1921 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     1981 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2041 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2101 gggccgccag gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2161 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2221 tctcgaagtc gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg
     2281 cagtggttaa agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa
     2341 atgcaaatgg taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg
     2401 cagattcagg aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag
     2461 gacaggttac gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcat
     2521 tttcgacacc agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg
     2581 atttttatga acaagtatat caaacagtgg tgctttgctg tgtttatgct ctcgttaagt
     2641 agtgtagcac ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt
     2701 acgcttgatt tctcg
//
LOCUS       DAJTNP010000225         2689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTNP010000225 DAJTNP010000000
VERSION     DAJTNP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..545)
                     /locus_tag="OO160_004155"
     CDS             complement(<1..545)
                     /locus_tag="OO160_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526031.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO160_004156"
     CDS             complement(1207..2196)
                     /locus_tag="OO160_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526032.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2689)
                     /locus_tag="OO160_004157"
     CDS             complement(2280..>2689)
                     /locus_tag="OO160_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526033.1"
                     /translation="YPLSLAIKTLIPGYLSGLPSTSREAMELLEALAEAGVTIGNFFS
                     NDLMTAYQRRQMNKRAEAERIAKELASQKERTREMFMTEDEWQKELQRREQVKAERRT
                     YGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          699 a          625 c          655 g          710 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctcgc ggctggtgga cggtaagcct
     2641 gacaggtagc ccggaatgag ggtcttgata gcgagcgaca acggatagg
//
LOCUS       DAJTNP010000226         2673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTNP010000226 DAJTNP010000000
VERSION     DAJTNP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..169
                     /locus_tag="OO160_004158"
     CDS             <1..169
                     /locus_tag="OO160_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5526034.1"
                     /translation="DVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQD
                     VPGDQKEQIPD"
     gene            208..1011
                     /gene="agaC"
                     /locus_tag="OO160_004159"
     CDS             208..1011
                     /gene="agaC"
                     /locus_tag="OO160_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5526035.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1001..1792
                     /gene="agaD"
                     /locus_tag="OO160_004160"
     CDS             1001..1792
                     /gene="agaD"
                     /locus_tag="OO160_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5526036.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1793..2548
                     /locus_tag="OO160_004161"
     CDS             1793..2548
                     /locus_tag="OO160_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5526037.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          659 a          625 c          667 g          722 t
ORIGIN      
        1 agatgtcaac gtcggcaata tgcatttctc ggaagggaaa aagcaaatca gcagtaaagt
       61 ttatgtcgat gaccaggatc tcgcggactt acgttttatt aaacaacgtg gcgtgaatgt
      121 tttcattcag gacgtccctg gcgatcaaaa agaacaaatc cctgactaaa tctaaaatcg
      181 ccttaatatt ggtttgaggt aataaaaatg catgaaataa ccctacttca gggattatcc
      241 ctagcggcgt tagttttcgt tctggggatt gatttttggc tggaagcctt atttttattc
      301 cgcccgataa tcgtttgtac cctaactggc gctattctcg gtgatattca gactggctta
      361 attaccggtg gtctgacaga gttggctttc gccggattaa cccctgcagg tggtgttcag
      421 ccgcccaacc cgattatggc gggtctgatg accaccgtca ttgcatggtc tacgggcgtt
      481 gatgccaaaa cagcaattgg tcttggcctg ccgtttagtt tgttaatgca gtacgtcatt
      541 ctgttcttct attccgcttt ctcattattt atgaccaaag ccgataaatg cgcgaaagag
      601 gcggatacgg cagcgttttc ccggcttaac tggacaacga tgctcatcgt cgcttcagcg
      661 tatgcggtga ttgctttcct ctgtacttac ctggcacaag gggcgatgca ggcgctggtg
      721 aaagcgatgc ccgcctggtt gacccacggc tttgaagtgg ctggcggtat tctgcctgcc
      781 gttggttttg gcttgctgct gcgcgtaatg ttcaaagcgc aatatatccc ttacctgatc
      841 gccggtttcc tgtttgtttg ctacatccgg gtcagcaacc tgttgccggt tgccgtactg
      901 ggcgcaggct ttgcggtgta tgagtttttc aatgcgaaat cccggcagca agcgcaaccg
      961 cagcccgttg ccagtaaaaa tgaagaagag gactacagca atgggatctg aaatcagtaa
     1021 aaaagatatc acccgtctgg gctttcgttc gtcgctgctg caagcgagct ttaactacga
     1081 aaggatgcag gcgggcggtt ttacctgggc gatgttgccg atcctgaaaa agatttataa
     1141 ggacgacaaa ccgggcttaa gcgcggcgat gaaagataac ctcgaattta ttaataccca
     1201 cccgaatctg gtcggattcc tgatggggtt attaatttcg atggaagaaa aaggagaaaa
     1261 ccgcgacacc attaaaggcc tcaaagtggc actgtttggc ccaatcgccg ggattggcga
     1321 tgcgattttc tggtttactt tgttgccgat tatggcggga atttgctcat catttgccag
     1381 ccagggaaac ctgctggggc cgattctatt tttcgccgtt tacctgctta tctttttcct
     1441 gcgcgtcggc tggacccacg tcggttattc agtcggcgtg aaggcgatcg ataaagtgcg
     1501 agagaactcg cagatgattg ctcgctcggc aaccatcctc gggatcacgg taatcggcgg
     1561 gctgatcgct tcgtatgtgc atattaacgt ggtgacatcg tttgccatcg acagtaccca
     1621 cagcgtcgcg ctgcaacagg atttcttcga taaagtcttc ccgaacattt taccgatggc
     1681 ctacaccctg ctgatgtatt acttcctgcg ggtgaaaaaa gcgcatccgg tgctgttaat
     1741 cggcgtgact tttgtgctct ctattgtttg ttccgcattc ggcattttgt aaatggaacg
     1801 aggcactgct tctggcggtg cctctttact taaggaattt catcctgtgc aaacccttca
     1861 gcaagttgaa aactatacgg cgttaagtga acgagccagc gaatatttat tggctgtgat
     1921 ccgtagcaaa ccggatgccg tgatttgcct ggcgaccgga gccacgccat tactgacgta
     1981 tcattatctg gtagaaaaaa tccaccagca gcaggttgat atcagccagc tcaccttcgt
     2041 gaagctcgac gaatgggtgg atctgccatt aacgatgcct ggcacctgcg aaactttcct
     2101 acaacagcat atcgtgcagc cgctggggct acgtgaagac cagctcatca gctttcgctc
     2161 cgaagagata aatgagacag agtgcgaacg ggtaacgaac ctgattgcgc gcaaaggcgg
     2221 tctggattta tgcgttctcg gattggggaa aaacggtcat cttgggctga acgaaccggg
     2281 agaaagccta caaccggcct gccatatcag tcaacttgat gccagaacac agcaacatga
     2341 gatgttaaaa accacgggtc gccccgtgac tcgtgggatc accttaggcc tgaaggatat
     2401 tctcaatgcc cgcgaggttt tgttattggt gactggcgaa ggaaagcagg atgcgacaga
     2461 acgttttctc acggctaaag tctccaccgc tatcccggct tcatttttat ggctgcacag
     2521 taactttatt tgtttaatta atacctaacc agatgaaaaa tttgctcaaa atttttaaat
     2581 taacttattc aacagtcacg cattatatta aataacattt gactgggttg aacataacgc
     2641 caatagcaaa aggagttatg ttcaacaatt taa
//
LOCUS       DAJTNP010000227         2673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTNP010000227 DAJTNP010000000
VERSION     DAJTNP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..567
                     /locus_tag="OO160_004162"
     CDS             <1..567
                     /locus_tag="OO160_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5526038.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="OO160_004163"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="OO160_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5526039.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OO160_004164"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OO160_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5526040.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="OO160_004165"
     CDS             2169..2405
                     /locus_tag="OO160_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5526041.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2444..2530)
                     /locus_tag="OO160_004166"
     tRNA            complement(2444..2530)
                     /locus_tag="OO160_004166"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2494..2496),aa:Leu,seq:cag)
     gene            complement(2565..2651)
                     /locus_tag="OO160_004167"
     tRNA            complement(2565..2651)
                     /locus_tag="OO160_004167"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Leu,seq:cag)
BASE COUNT          681 a          606 c          683 g          703 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     2521 ccaccttcgc acagtcatct tacttttttt gatatcgcct cgtttggtgc gagggggggg
     2581 acttgaaccc ccacgtccgt aagaacacta acacctgaag ctagcgcgtc taccaattcc
     2641 gccaccttcg cccagtgcga gcaatatcaa cgt
//
LOCUS       DAJTNP010000228         2625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTNP010000228 DAJTNP010000000
VERSION     DAJTNP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            623..1222
                     /locus_tag="OO160_004168"
     CDS             623..1222
                     /locus_tag="OO160_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5526042.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1595..1978
                     /locus_tag="OO160_004169"
     CDS             1595..1978
                     /locus_tag="OO160_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526043.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1975..2400
                     /locus_tag="OO160_004170"
     CDS             1975..2400
                     /locus_tag="OO160_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526044.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          651 a          637 c          661 g          676 t
ORIGIN      
        1 ggcttcaccc gtgttaatat cctggataac tgtgtttgaa ggatttctgt taataatctg
       61 ctcctgcaac tcacctgatg agtattagtg gtaatacata atcttactcg taaaagagta
      121 taccttatgt cattaaacag tcttccggcg tgttgttcaa tcctgtacct ctgttactta
      181 tctggtacgt tgatatgtgt aatgctacgt taattgcaat atctggttat ggctttatac
      241 cttcagcatt taaaatattt tattaccgga gaaacaggga agtggaaaga cttttgggat
      301 aatgtcaggc cattctgacg ttaatcacag taccaaataa cgagagggta atggaagtta
      361 aaacctgcgt aagttcctct gttgttcact ggaaaaagtg attgcccaca cgcgttataa
      421 aggcctttcc gctgcagcct ggttaacaga gcttcaggcg ttatatgcgc tgtatggctg
      481 ttacgtatgc tgttctttgc ctggttctga tacccgtgga agtcgttacc ggaggaaaca
      541 cgttcaccct actacctgag aacatcttct tcaaaaatta cggcaacgct tcttcctgtg
      601 ttacagaaca cgaggaagaa acatgcaaac ttataacacc aatcccgctt acgaaatggc
      661 tccccagctc ctggagcact ttaaccagca tctggattca ttattcgggg tctattcgaa
      721 actgttaccg ttcagaatgg attttgctta ccgaaaaaac accctcagct acagatgtgc
      781 ctgcaggtac gccatgtgcg cggagatgct gcaactgatt aacgaggtgg gagagaaact
      841 ggtcgggtat gcctgggtga tggaatatac agaacgtaaa gggcttcaca ttcattttgt
      901 gggctatctg aacggccaga gccacaggag ttcgtatctg gtatccagat tgatgggaga
      961 tatctggagg cgggttaccg agggtaatgg ttactaccac tggtgccggt ttaataaaaa
     1021 ttatccggta aacattaacc acgtcattca ctattcagat cacaaagcgg tgaatgattt
     1081 acgctatgcc atcagctacc tggccaaacg ggaacagaaa gaatgtggca tcatcctgaa
     1141 atgcagcggg cttcctgaaa aaagtaaccg gggtcgacca cgactggaca gccccctgcc
     1201 ggggatatgt gctctggtgt aaacccctga acgacaagta cgggctggcg ctaatctttg
     1261 ctgtcggttc cggcggccac ttcagcggca ctgcgcattt tttacggcgt ggtcattccg
     1321 tatgaaccat acggagattt aaccatgaca cattctttac cattccttcc ggcaaccgat
     1381 acagcacatc ccccaatctc catctctgct tactgactgt tttttacgtc agtgggccat
     1441 tgtgtgacaa ccacagtgct gtaacagcaa ccagcctgct ttacccaaac tccagtccag
     1501 attttaactg aaccgccatt cacaccctga ccacgctgcc cgtaccggga cggctgttgc
     1561 ctgcgtgtcg tctggcggct gaggaacata tactatgtac gcaaaatctt ttctcgcact
     1621 tgatggcaac ggacgtctga ccggtgcccg taccgcacag actgcacctt acgatagcta
     1681 cacctgccac ctttgcggca gtgcactcag ataccacccg caatacgaca ctgaacggcc
     1741 ctggtttgaa cacgctgacg aagggctgac ggagcacggt catgagtgcc cttatgtcag
     1801 gccggaacgc agagaaatac ggttgattaa acgtctgcag caattcgtcc cggatacctt
     1861 acccgtagta cgtaaagcca gctggcactg caggcaatgt caccacgatt attatgggga
     1921 gcagtactgc acacactgcc agaccgggcg cttcagtgag gaggggggag cagaatgaac
     1981 ggtataaaaa cggggctggg gatcacgccg ggtgagcaca ttatcagtgc cgactctgcg
     2041 ctcagcagaa atatccggca gtgtttctgc ctttcctgcc atggccggtt gattctgcag
     2101 acagatgctc agggggcgtg gtttgagcat gatttacatg ccctgagtga gcaacagaag
     2161 gcggactgtg tggtgctgaa tccggagaag tcccaccctt acattgagga tatggcgatg
     2221 tttttatcgc cattaccggt cgtgctggag tggcattgtg tgatgtgtga gcagtttttt
     2281 catgggaaga agttctgtga ggcctgtggt acggggattt attgcagagc ggtatgtacg
     2341 aagtctgttt acagctacca gccagacttg tttgaggact gcggaggtgc gagtacttaa
     2401 tccgaaatgc ccgacctgct cagggcgttc agaggtattg ccaacctgta ttttatctgg
     2461 tgtttcgcac ccaccccgtt cgtgacacaa aggcgcgaca ccggatatcc gcagtcctgc
     2521 ttacctgaat aaaacccggg gtattgattg gtagtgtggt gcggaatcgt accgtgatct
     2581 cgcattcgcc gaattcgcac tgtggtgtca atcaaccata cgcga
//
LOCUS       DAJTNP010000229         2562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTNP010000229 DAJTNP010000000
VERSION     DAJTNP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(64..822)
                     /locus_tag="OO160_004171"
     CDS             complement(64..822)
                     /locus_tag="OO160_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5526045.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO160_004172"
     CDS             complement(830..1933)
                     /locus_tag="OO160_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5526046.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2562)
                     /locus_tag="OO160_004173"
     CDS             complement(1943..>2562)
                     /locus_tag="OO160_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5526047.1"
                     /translation="VVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          706 a          681 c          599 g          576 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac ag
//
LOCUS       DAJTNP010000230         2489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTNP010000230 DAJTNP010000000
VERSION     DAJTNP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..756)
                     /locus_tag="OO160_004174"
     CDS             complement(<1..756)
                     /locus_tag="OO160_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5526048.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDN"
     gene            complement(1355..1603)
                     /locus_tag="OO160_004175"
     CDS             complement(1355..1603)
                     /locus_tag="OO160_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5526049.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1958..2305)
                     /locus_tag="OO160_004176"
                     /pseudo
     CDS             complement(1958..2305)
                     /locus_tag="OO160_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            complement(2332..>2489)
                     /locus_tag="OO160_004177"
     CDS             complement(2332..>2489)
                     /locus_tag="OO160_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5526050.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          634 a          475 c          602 g          778 t
ORIGIN      
        1 gttatcacta atatctattg aacattcatt cctcagatta agaatgcttt ccgggatatg
       61 acttatctga tttatgtcaa aataaccgtt ccttaaattt tccggcaggt atggaatatc
      121 ggttaacata ttgtcgattg cccatatgga gtgaatgttg tgaggtagag gggggagttc
      181 actaagaaga ttccctattg cgctaatctc ttgtaaagaa aatggtaaag gtggtaaaac
      241 tgccagccca ttacatgata catctaatgt ttccagtagt tctggtaaaa caggaagaga
      301 acataattga ttatttgaga catgaagctc cttcaaagat atggggagtg tgggtagtgt
      361 gattagttga ttgtgggacg cattcaataa tttaagtcct tgaggcaaag caggcagttc
      421 aataagtctg ttatagctga cattaagttg tgtaagggac gcaggcaatg gggagattaa
      481 gcttaaatta tttttactta tattaattga tttaattccc ggggggattt caggtaatgt
      541 tgtcaggcct aattcagaca agtccaggtt cgtctcttgg ttttgtagac atgatactag
      601 tcgctgaaaa gcgatgtctc gttgttcttc ttgtatgcgg ttatttttcc attcagtcca
      661 atgggttaga taattttcat atgcgcggct agtgtcgatt gaataagtgg agaacgaatt
      721 tgaaattaat ctgtgattgt tatttgtcgg gagcataaat atcagggtgt attctttatg
      781 ttgagaggtg ttggattctt ttttatctgc tttgcaatgt tattggttcc cttcagcagt
      841 agacaggaac ttctggagga ggcggatata tagtttgtgt aagcatatta actccatatg
      901 ttatatattg aagaacttct gctttacact atcctaaact ggatcggttt aatgtaaaaa
      961 aacgatactt tttggggggg ggcaattttt ccttaaggta tgaaaaaagg agctgaagct
     1021 atataatagc tattaatgcc actggtatca gtgaatcatg tatataaaaa actgtgttaa
     1081 ggaggctgtc cattagcggt tttttattct tctggaaccg cggcatcttt gcgtatcttt
     1141 ttgtcttctc accgtctggt cggtgttttg ctgagactgc taatttcctg tctttgctag
     1201 tgctgtactg gtactgccca atcatgattg gcgggggcgg gcggagttga gaccgcagac
     1261 acgtcgtatg caagaacgtg ctgcggctgg ctggtgaact tccgatagtg cgagtattga
     1321 atgatttcca gccgttactg attttacgtg ctaattagtg aacaaaccac tcgtcagcag
     1381 actcccaggt atctttcaga gtttcctgaa caaaagtttt agctgaatct ttatcggcgg
     1441 tgcgcgtaac agaaaggcca tcgttgctgg tggcttttac gatcacctct acatcgtcat
     1501 aacgtttact gatgcgtcgg gttaattctt cctttaacgc atccacagca ccggttggca
     1561 ttttagtcat tttctctttg gctatgcaga tttcaaaacg cataaaaatc cctctgcact
     1621 gtgtttttat acagtattat ttttaactgt atggataaac agtgtcaaga gtgagggaaa
     1681 gtgatttttt ggggcatgca tggggcaaaa atatttgttg tggggcattt tggggcatga
     1741 ttgggacact ttgttacata tgaactttgc cgattttcat atgacatggc aatccaacgc
     1801 tttgattatt ctaaagaaaa cacatgctct tgggcgttct ttagtgattt ttaaaaatcc
     1861 cgtatcacgc agttaaagtg gcgggcatac tcttcaaggc tggtgatccc caaccgcacc
     1921 catttaggat gtgaccattg cggtagaccg atgtaaatca taacgcgtga aggatctctt
     1981 ccacgctacg cacacgggcg atgcgcgggt agatatgatt aatgctgtta ttgtgttgct
     2041 cggcgctggc ggcactacag gcgtcttcgg caatcaccag attaaagcca agttcccagg
     2101 cattgcgggc ggtggattca acaccgatat tggtcgagat cccacataac actattgtat
     2161 cgatacctcg gcggcgcagt tgcaactcca gatccgtacc gtaaaacgca ccccactgac
     2221 gtttgatgat ttcgatatca ctgtcagttg cacctaatgc agctggatgt tgccaccagt
     2281 tttcgggcaa cactttagcg ggggagtgcc cctattaagt tgtcaagcat gttatgaccc
     2341 ctgcggggta taaaaagtcc cgtcgcgcat catggcgaac agaacgtcgc agcgtcgtct
     2401 cgccagggcg ataagcacct gattgtgtcg ttttccctga ctcattttgc gggtgtagta
     2461 agccctggag agcggatccc tgagcgcgg
//
LOCUS       DAJTNP010000231         2418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTNP010000231 DAJTNP010000000
VERSION     DAJTNP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..243
                     /locus_tag="OO160_004178"
     CDS             <1..243
                     /locus_tag="OO160_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5526051.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="OO160_004179"
     CDS             complement(332..844)
                     /locus_tag="OO160_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5526052.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..1803
                     /locus_tag="OO160_004180"
     CDS             1039..1803
                     /locus_tag="OO160_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526053.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1754..>2418
                     /locus_tag="OO160_004181"
     CDS             1754..>2418
                     /locus_tag="OO160_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5526054.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          681 a          539 c          614 g          584 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgctg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga
     1741 gagcgtgacc tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg
     1801 tagtttctgg actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa
     1861 atcatgggat gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg
     1921 ttgtgcccgt cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca
     1981 gatagacagg tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg
     2041 ggttactggc tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc
     2101 actggagggg atcgatatgt tgtggctgga agaagcccac gcactgacgg aataccagtg
     2161 gaaaattctg gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc
     2221 cggacttgtt actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct
     2281 catccgcaaa attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat
     2341 cgacgcggcg cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga
     2401 gtctgatgat gatgcggc
//
LOCUS       DAJTNP010000232         2226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTNP010000232 DAJTNP010000000
VERSION     DAJTNP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..828
                     /gene="proB"
                     /locus_tag="OO160_004182"
     CDS             <1..828
                     /gene="proB"
                     /locus_tag="OO160_004182"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5526055.1"
                     /translation="IYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINEN
                     DAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGI
                     DDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVG
                     TLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRG
                     EVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            840..2093
                     /gene="proA"
                     /locus_tag="OO160_004183"
     CDS             840..2093
                     /gene="proA"
                     /locus_tag="OO160_004183"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5526056.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          529 a          562 c          643 g          492 t
ORIGIN      
        1 atttatggca ttcacgtcgg gcaaatgctg ctgacccgtg ctgatatgga agaccgtgaa
       61 cgcttcctga acgcccgcga caccctgcgt gcgttgctcg ataacaatat cgttccggta
      121 atcaatgaga acgatgctgt cgctacggca gagattaagg tcggcgataa cgataacctt
      181 tctgcgctgg cggcgattct ggcgggtgcc gataaactgt tgctgctgac cgatcaaaaa
      241 ggtttgtata ccgctgatcc gcgcagaaat ccgcaggcag aactgattaa agatgtttac
      301 ggcattgatg acgcactgcg cgcgattgcc ggtgacagcg tttcaggcct cggaactggc
      361 ggcatgagta ctaaattgca ggccgctgac gtggcttgcc gtgcgggtat cgacaccatt
      421 attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga tggaaggcat ttccgtcggt
      481 acgctgttcc atgcccaggc gactccgctt gaaaaccgta aacgctggat tttcggtgcg
      541 ccgccggcgg gtgaaatcac ggtagatgaa ggggcaactg ccgccattct ggaacgcggc
      601 agctccctgt tgccgaaagg cattaaaagc gtgactggca atttctcgcg tggtgaagtc
      661 atccgcattt gcaacctcga aggtcgcgat atcgcccacg gtgtcagtcg ttacaacagc
      721 gatgcattac gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat actgggatat
      781 gaatacggcc cggttgccgt tcaccgtgat gacatgatta cccgttaagg agctggccga
      841 tgctggaaca aatgggcatt gccgcgaagc aagcctcgta taaattagcg caactctcca
      901 gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga actggaagca caaagcgaaa
      961 tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg agccaatggc cttagcgaag
     1021 cgatgcttga ccgtctggca ctgacgcccg cacggctgaa aggcattgcc gacgatgtac
     1081 gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat cgatggcggc gtactggaca
     1141 gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt tattggcgtg atttatgaag
     1201 cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct gaaaaccggt aatgcggtga
     1261 tcctgcgtgg tggtaaagaa acctgtcgca ctaacgcggc aacggtggcg gtgattcagg
     1321 acgccctgaa atcctgtggc ttaccggcgg gtgccgtgca ggcgattgat aatcctgacc
     1381 gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg atcccgcgtg
     1441 gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac aatcccggtg atcacaggtg
     1501 gtataggcgt atgccatatt tacgttgatg aaagtgcaga gatcgctgaa gcattaaaag
     1561 tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa tacggttgaa acgttgctgg
     1621 tgaataaaaa catcgccgat agcttcctgc ccgcattaag caaacaaatg gcggaaagcg
     1681 gcgtgacatt acacgcagat gcagctgcgc tgacacagtt gcaggcaggc cctgcgaagg
     1741 tggtggcggt taaagccgaa gagtatgacg atgagtttct gtcattagat ttgaacgtca
     1801 aaatcgtcag cgatcttgac gatgccatcg cccatattcg tgaacacggc acacaacact
     1861 ccgatgcgat cctgacccgc gatatgcgca acgcccagcg ttttgttaac gaagtggatt
     1921 cgtccgctgt ttacgttaac gcctctacgc gttttaccga cggcggccag tttggtctgg
     1981 gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg gggctggaag
     2041 cactgaccac ttacaagtgg atcggcattg gtgattacac cattcgtgcg taaataaaac
     2101 cgggtgatgc aaaagtagcc atttgattca caaggccatt gacgcatcgc ccggttagtt
     2161 ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc gatatagctc
     2221 agttgg
//
LOCUS       DAJTNP010000233         2207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTNP010000233 DAJTNP010000000
VERSION     DAJTNP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..406
                     /locus_tag="OO160_004184"
     CDS             <1..406
                     /locus_tag="OO160_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5526057.1"
                     /translation="QLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQI
                     IKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGRNIFQS
                     DHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            430..720
                     /gene="lsrG"
                     /locus_tag="OO160_004185"
     CDS             430..720
                     /gene="lsrG"
                     /locus_tag="OO160_004185"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5526058.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            760..1518
                     /gene="tam"
                     /locus_tag="OO160_004186"
     CDS             760..1518
                     /gene="tam"
                     /locus_tag="OO160_004186"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5526059.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1522..>2207)
                     /locus_tag="OO160_004187"
     CDS             complement(1522..>2207)
                     /locus_tag="OO160_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526060.1"
                     /translation="RYVEARKLWGQLMIASRSLLREVKTTLPDSASVREFARLQIAFA
                     HCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRILLIMGEWLAVQRRNGQLSDI
                     LFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLFCIMLPFALVVDLHYMT
                     PFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICNAIEIDLLQMNDEAEIPAKI
                     LPDRHYQLT"
BASE COUNT          567 a          526 c          587 g          527 t
ORIGIN      
        1 tcagctggtc gatgccggaa tgaaagtggg aatgccgacc atggccgtga ctggcgtggg
       61 caaagatatg gtgcgcgatc agcgttattt ctcgctcgcg actcgaatcg ccgctgaaat
      121 gggggcgcaa attatcaaaa cctattatgt cgaaaaaggt tttgaacgga ttgttgccgg
      181 atgtccggta cccattgtta ttgctggcgg taaaaaatta ccggagcgcg agacgctgga
      241 aatgtgctgg caggctatcg atcagggcgc ttctggtgtg gatatggggc gtaatatttt
      301 ccagtctgac catccggtgg cgatgatgaa agccgtacag gcggtggttc accataacga
      361 aacggctgat cgggcatatg aactctatct gagtgaaaaa cagtaactgc ggatttaagg
      421 agaagaatta tgcacgtcac actggttgaa attaacgttc atgaagacaa ggttgacgag
      481 tttatcgaag tttttcgcca gaaccacctg ggctctgtac aggaagaagg taatttgcgc
      541 ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt atatttacga agcctataaa
      601 gatgaagaca cagtggcatt ccataaaacc acgcctcact acaaaacctg tgtcgcgaaa
      661 ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt tcaatgggtt gatgccgtga
      721 ggctacaatt ggggtaacgc gctgacggga gtaaaaaaaa tgtctgactg gaacccctct
      781 ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagtg
      841 ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa cagcaccgcc
      901 ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc tccggcgatg
      961 attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga catccgcaac
     1021 tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc
     1081 gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg cgtgctggca
     1141 gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcttgg
     1201 gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc ttactacgat
     1261 attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta tcaccagatg
     1321 ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc gtggttacag
     1381 gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat gctggaagag
     1441 cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct gtttattgtt
     1501 gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa gaatttttgc
     1561 tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc
     1621 atccagcggt aaatcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca
     1681 atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca tgtaatgcag
     1741 gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat
     1801 cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc
     1861 tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa gctgtccatt
     1921 gcggcgctga accgccaacc attctcccat tattaacaag atacggttag ctggagagtt
     1981 cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca cttccgcctg
     2041 tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa
     2101 ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta acgaccgtga
     2161 ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacggg
//
LOCUS       DAJTNP010000234         2153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTNP010000234 DAJTNP010000000
VERSION     DAJTNP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO160_004188"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO160_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5526061.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="OO160_004189"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="OO160_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1984)
                     /locus_tag="OO160_004190"
                     /pseudo
     CDS             complement(1294..1984)
                     /locus_tag="OO160_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          630 a          398 c          494 g          631 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     1501 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     1561 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc
     1621 cagtgttgtt gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg
     1681 gcaacatcag cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg
     1741 tccgtatgac ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc
     1801 cgttatgtgc catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt
     1861 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
     1921 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
     1981 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
     2041 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
     2101 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DAJTNP010000235         2130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTNP010000235 DAJTNP010000000
VERSION     DAJTNP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..837
                     /locus_tag="OO160_004191"
                     /pseudo
     CDS             1..837
                     /locus_tag="OO160_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            952..1293
                     /locus_tag="OO160_004192"
                     /pseudo
     CDS             952..1293
                     /locus_tag="OO160_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1276..2113
                     /locus_tag="OO160_004193"
                     /pseudo
     CDS             1276..2113
                     /locus_tag="OO160_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          526 a          516 c          640 g          448 t
ORIGIN      
        1 acaaatggta gtgtccgcta ttgccagtac acctcagttc tgggccttgc ccgtagcagt
       61 tatttttatc accgggctgc actgaaagcc ggtgataaat acgccacgat acgtaagatg
      121 ctgacagata tctttaacag taattaccag tgttatggct atcgtcgcct gcatgcgatg
      181 ctcaggcatg agggggggcg gctatcagaa aaggttgtac gcagacttat ggtggaggaa
      241 cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct catattgcgg agaaatcgga
      301 ccggctccgg ataaccttat cgccagagat tttaaggcgg agcaacctaa tcagaaatag
      361 ctgacagata tcacggagtt ccagctccct gcaggtaaag tctggctgtc accagtggtg
      421 gactgcttcg atggaaaagt tgtgagctgg tctctcagta cacgccctga tgctgaactg
      481 gtcaacacta tgctggatag cgctgtcgaa acgttaaatg ctggcgaacg accggtgata
      541 cacagtgaca gaggtgggca ttatcgctgg ccaggctggc tggaaagagt gaatgcagca
      601 ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg ataatgctgc atgcgaaggc
      661 tttttcggca gactgaaaac ggaaatgtat tatgggcgta aatggtcggg catcacgcca
      721 gaaaagttca tgcagcaagt ggatgcttac atcagatggt ataacgagcg gcgtataaaa
      781 ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac aatgcgggct ggaatgataa
      841 agcagtccag gaaatcgtcc gcatcccccc ttcaccattt ggattgtccg ctccacccaa
      901 catgttgttt ccttaaggtt cctacaccac aaaggaaact ctcatgctgc gcagtggatg
      961 gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc agacaccgga acagcgcttc
     1021 acgcaggaac aggagcatct gcagccgctg ctgggtacgg acttcgatat ccgtcatgtc
     1081 tcctgggatg gctatatcga ggttggcggg aaccgttaca gtgtgccgga aagcctgtgt
     1141 ggtcagctgg tatcaatacg gatctcgctg gatgatgaac tgcggatcta cagtaatgag
     1201 caacaggtag catcgcaccg tctctgttcg gctgcatacg gctggcagac cgtgccggag
     1261 catcacgctc cactctggca gcaggcttcg gagcgcatgg ccgaatggct gggggagata
     1321 cagaaacggg taatcactgt ctgtgaccgg gaagcggata tctggcatta cctgtattat
     1381 aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat cgccggctgg aagaggcacc
     1441 cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca ggaagccaca cgctgaatgt
     1501 gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg ttcatcagtt acagcgaagt
     1561 cagcataaaa aatcccgaca acagcggcca ggcgctcccg ctcacgtatg tctgttgccg
     1621 ggagcaggca gaggacggtg cctgctggca tctgctgacg tcaggaaaag cggcgagtgc
     1681 cgccgatgca cgacgtattg tcagccatta cgagcgacgc tggctgacag aggaatacca
     1741 caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa tgcagacccg ggataacctt
     1801 gagcgcatgg tggttatcct ggcgtttata gcggtacggg tgctgggcct gcggcaggga
     1861 ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa tactgacgcc gacagagtgg
     1921 aaactactgt gggttaagct ggaaggaaag ccgcttccgg tgcaggcacc gaccctgaaa
     1981 tgggcctggg gcgatggcat gacagcaaac gcacaggtcg tcccggttgg ggcgtcatgt
     2041 gggatggctg gtcagacttc aggatatggt tgagggttac ctggtgatga agtctcttga
     2101 tcaggagctc tgatcaagag acagcagctt
//
LOCUS       DAJTNP010000236         2105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTNP010000236 DAJTNP010000000
VERSION     DAJTNP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            264..568
                     /locus_tag="OO160_004194"
                     /pseudo
     CDS             264..568
                     /locus_tag="OO160_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            737..2101
                     /locus_tag="OO160_004195"
     CDS             737..2101
                     /locus_tag="OO160_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5526062.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          547 a          417 c          479 g          662 t
ORIGIN      
        1 gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat taactagcca
       61 atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa ttacctatac
      121 ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt tgtgcgcggt
      181 cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg ttagcgggcc
      241 gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg ctcacaccag
      301 tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt actcctgtct
      361 ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac agggctggta
      421 tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt acgcgtaaac
      481 aactaaataa aaatatctta aacatgcaag taattttggt attctcgata caagaataaa
      541 tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc atctttacat
      601 ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg tgataatgca
      661 atactttgag tgattatata atgataagta aacaggtttg cgacaattgg ttatcagtat
      721 tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa gattaccctc
      781 tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca gcattttcct
      841 cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg gtgtgctgca
      901 catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct atggtatatc
      961 aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt tgtctcttgg
     1021 tttattcctg tccggactgg taaattttct gataggatat attcccggga tcatcacatc
     1081 cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta tgggatatcc
     1141 tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca cctgggccac
     1201 actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga ttggtttcag
     1261 ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta tcttcccagg
     1321 agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca gacctgtatc
     1381 agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc cggctaaagg
     1441 ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata tcatccgaaa
     1501 taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga gatacggtat
     1561 tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta aggatatggg
     1621 aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg ctggtatgct
     1681 tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa caaatacttt
     1741 atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat caggcaatga
     1801 atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt atggtccggt
     1861 gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg gagccgcatc
     1921 agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac tgggaatggg
     1981 acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac tgtctgctgt
     2041 ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg aaaaagagta
     2101 atttt
//
LOCUS       DAJTNP010000237         2100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTNP010000237 DAJTNP010000000
VERSION     DAJTNP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            117..1154
                     /gene="curA"
                     /locus_tag="OO160_004196"
     CDS             117..1154
                     /gene="curA"
                     /locus_tag="OO160_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5526063.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1352..2017
                     /gene="mcbR"
                     /locus_tag="OO160_004197"
     CDS             1352..2017
                     /gene="mcbR"
                     /locus_tag="OO160_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5526064.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          578 a          446 c          561 g          515 t
ORIGIN      
        1 ggattcctcg atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc
       61 aatcccgtga gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg
      121 ggcaacaaaa gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg
      181 ttccggagaa tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt
      241 tactgcgcac agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc
      301 catcttattc accgccggtt gatattggcg gcgtgatggt cggcggtacg gtgagccgtg
      361 tcgtggagtc gaatcatcct gattatcagc ctggcgactg ggtgctgggc tacagtggat
      421 ggcaggacta tgacatatcc agtggtgatg acctggtgaa acttggcgat catccgcaaa
      481 atccatcgtg gtcgctgggt gtgctgggga tgccaggctt taccgcttat atggggctgc
      541 tggatatcgg tcagcctaaa gagggcgaaa cgttggtggt agctgcggcg acagaaccag
      601 tgggggcgac ggtggggcaa atcggcaaac ttaaaggttg cagagtggtg ggggtagccg
      661 gtggcgcgga aaaatgccgc catgctaccg aggtgttagg cttcgatgtt tgccttgatc
      721 accacgcgga cgattttgcc gaacaactgg cgaaagcgtg cccaaaaggt attgatatct
      781 attatgaaaa cgtgggcggt aaggtatttg atgcagtgct accattactt aatacatctg
      841 cgcgcattcc cgtttgtggc ttagtgagca gctataacgc tacagagcta ccacccggtc
      901 cggatcgttt acccctgttg atggcaacgg tgctgaaaaa acgtattcgc ttgcaagggt
      961 ttattatcgc tcaggattat ggtcaccgca tccatgagtt tcagaaggag atggggcaat
     1021 gggtgaaaga ggataaaatc cactaccgcg aagatattac tgatggttta gagaatgcgc
     1081 cacagacgtt tatcggcctg ctgaagggta aaaacttcgg caaagtggtg atccgcgtcg
     1141 cgggtgatga ttaaagtttc aacggcggcg taagccgcca taaaaatcca ggcaaaaagc
     1201 ttattttcag ctttaattaa ctaaccacgt cattgttctg ctgctaataa caacaaaggt
     1261 gggtggttta tgattattca ctttaataca ctaggtgaac tccttctgcc atgcaggcag
     1321 ggttggacag aaaacacgcg aaggaattaa aatgcctgga acgggaaaaa tgaaacatgt
     1381 cagtttgact ctgcaggttg agaacgacct gaaacatcag cttagtattg gcgcattaaa
     1441 acctggcgca cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga gtattacacc
     1501 tgtgcgtgaa gcattattac gtctggtttc ggtgaatgcg ctttctgtcg cacctgcaca
     1561 agcatttaca gtaccggaag tgaggcaaag tcagttagac gaaatcaatc ggatccgcta
     1621 cgaactggaa ttaatggcag ttgccctggc tgttgaaaac ctctcctcgc aagacatagc
     1681 agaacttcag gaattgctcg ggaaattaca gcaggcgcaa gaaaagggcg acatggaaca
     1741 aatcattaat gtgaacaggc tatttcgctt agcgatttat catcgctcaa atatgcccat
     1801 cctgtgtgag atgattgagc aactgtggat caggatggga cctgctttac attatcttta
     1861 tgaagcgatt aacccagcgg aattacggga gcgcatagaa aactatcatc tattactcgc
     1921 tgcgttaaaa gcaaaagaca aagagggatg caaacattgt cttgctgaaa ttatgcaaca
     1981 aaatattgct attttatacc agcaatacaa tcgttaaatt aaatgcccgg caaataccgg
     2041 gcatcaagta attactcaaa tcgccacgca atattcatgc cgacaccata atttcgttca
//
LOCUS       DAJTNP010000238         2070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTNP010000238 DAJTNP010000000
VERSION     DAJTNP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(220..384)
                     /locus_tag="OO160_004198"
     CDS             complement(220..384)
                     /locus_tag="OO160_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526065.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OO160_004199"
     CDS             1286..1570
                     /locus_tag="OO160_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5526066.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OO160_004200"
     CDS             1558..2043
                     /locus_tag="OO160_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5526067.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          537 a          432 c          592 g          509 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca
//
LOCUS       DAJTNP010000239         2035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTNP010000239 DAJTNP010000000
VERSION     DAJTNP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            6..224
                     /locus_tag="OO160_004201"
     CDS             6..224
                     /locus_tag="OO160_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5526068.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO160_004202"
     CDS             226..591
                     /locus_tag="OO160_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5526069.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO160_004203"
     CDS             588..1253
                     /locus_tag="OO160_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526070.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO160_004204"
     CDS             1253..1618
                     /locus_tag="OO160_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5526071.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          556 a          419 c          541 g          519 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggtt
//
LOCUS       DAJTNP010000240         2035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTNP010000240 DAJTNP010000000
VERSION     DAJTNP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..559)
                     /gene="yehT"
                     /locus_tag="OO160_004205"
     CDS             complement(<1..559)
                     /gene="yehT"
                     /locus_tag="OO160_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5526072.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRT"
     gene            complement(556..>2035)
                     /gene="btsS"
                     /locus_tag="OO160_004206"
     CDS             complement(556..>2035)
                     /gene="btsS"
                     /locus_tag="OO160_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5526073.1"
                     /translation="NTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSMGGMTALSCMIS
                     TIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRL
                     VSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNE
                     VNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEV
                     VYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGE
                     GIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQAS
                     QLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQ
                     QLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASG
                     LGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          494 a          563 c          474 g          504 t
ORIGIN      
        1 gtgtacgact ttccaaggta cgtaatgtca attcggtaaa gccctctttc ccttcgtggc
       61 tggtaacgta gacaccgctc atccgactgc tgacaaatgc cacatctttc atttgcagca
      121 aataaatccg actatgcccc gtacaaggga taaatttcag cgcctgttga ttttccggta
      181 acagcgaaac atcctgcttg ctgcgctcct gacgcaatcg cgccagcgtt ttctccagtc
      241 gcgcttcatc aattggcttc agcagataat caaaggcatg ttcttcaaag gctttaatag
      301 cgtattcgtc aaacgcagtg agaaaaacaa tatacgggcg atgttccggg tcaagcatcc
      361 ccaccatttc cagaccactg atgcgcggca tctggatatc gagaaacagc acatccgggc
      421 gcagtttatg caccgcgccg atcccttcca cggcgtttga acactctcca acgatttcaa
      481 tatcgctctg ctcctgcaaa aatatacgca ggttctcccg tgctaacggt tcatcatcga
      541 caattaagac tttaatcatg cctcgtccct ccatggtagt cgtaacgtta ttcgggtgta
      601 actatcaggc tcacaggcga cgcttattcc atagtcatcg ccaaaccgtt cacgtaaacg
      661 cttatccacc agattcatcc ccagcccact ggcattggtt accggttgat acaaaccggc
      721 attgtcttcg atctccagca tcaaatgttg cccctcacgt cgggcgctga ttgccactcg
      781 ccctgtatcc agcagttgtg atgtcccatg tttaatggcg ttttccacta tcggttgcag
      841 ggtaaacgcg ggcaattgct gctgggataa ttcttgcgga atagcaatgt tgacctgcaa
      901 ccgcgactgg aagcgcgcct tttcaatttg cagataagcg ttcacatgtt caatttcgtc
      961 ggcgagagta acaaactccg aaggccgctt taagtttttg cggaaaaaag tggaaagata
     1021 ctgcaccagc tggctggcct gttcgctgtc gcggcggatc accgctttaa tggtgttaag
     1081 cgcattaaac aaaaaatgag gattcacctg ggcgtgaagc agtttgattt ctgactgggt
     1141 gagcatcgct ttttgccgct catattgccc tgcaagaatc tgcgccgaaa gcagttgcgc
     1201 aatcccctcg cccagcgtgc ggtttattga actgaataac cggtttttgg cttcatacaa
     1261 tttgatggtg cccatcacgc gctgattttc gccacgcaac ggaattacca gcgtcgaccc
     1321 cagtttgcat tgcggatgca aagagcaacg ataaggtact tcgttgccat cagcgtagac
     1381 cacttcaccg gtttcaatcg cttttaaggt gtaagtcgaa gaaatcggtt tgccgggtaa
     1441 atggtggtcg tcaccaattc cggtaaaggc cagcaatttc tctcgatcgg taatcgcgac
     1501 tgcaccaata tccagctcct gatacagtac ctgagccact ttcatgctgt tcacttcgtt
     1561 aaacccctgt cgcaaaatgc cttccgtcga ggctgccact ttcagcgcag tggcagaaaa
     1621 agccgaagtg tatttttcaa acatcgcgcg tttatcgagc aatatacgca taaacagcgc
     1681 cgcgccgacg gtattggtga ccatcattgg cgcagcaata ttactcacca gacgcaccgc
     1741 atcttcataa ggtcgggcga tcgcaaggat gatcagcatt tgcaccattt cagcgacgaa
     1801 cgtgacggca ccggcggtaa tggggttaaa gactttatca gtgcgcccgc gacggatcag
     1861 gatgctgtgt accaggccgc cgagcaatcc ttcaacgatg gttgagatca tgcaacttag
     1921 cgcggtcatg ccccccatcg aatatcgatg taagccgccg gtcagaccaa ccagcccacc
     1981 gacgaccgga ccgccgagta agccgcccat taccgcgcct atcgcacggg tattg
//
LOCUS       DAJTNP010000241         2034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTNP010000241 DAJTNP010000000
VERSION     DAJTNP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..2034
                     /gene="sat"
                     /locus_tag="OO160_004207"
                     /pseudo
     CDS             1..2034
                     /gene="sat"
                     /locus_tag="OO160_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          747 a          356 c          400 g          531 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc agat
//
LOCUS       DAJTNP010000242         1999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTNP010000242 DAJTNP010000000
VERSION     DAJTNP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..102
                     /locus_tag="OO160_004208"
     CDS             <1..102
                     /locus_tag="OO160_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526074.1"
                     /translation="CELVLPAGTLRLKGKLTPALLQTLIREIKGSSH"
     gene            102..449
                     /gene="tnpB"
                     /locus_tag="OO160_004209"
     CDS             102..449
                     /gene="tnpB"
                     /locus_tag="OO160_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526075.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            469..>1999
                     /locus_tag="OO160_004210"
     CDS             469..>1999
                     /locus_tag="OO160_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5526076.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RV"
BASE COUNT          478 a          551 c          578 g          392 t
ORIGIN      
        1 tgtgagctgg ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta
       61 ttacagacac ttatccgcga aataaaaggg agcagccact gatgatatct ctccctgcag
      121 gttcgcgtat ctggctggtt gccggtatca ccgatatgcg aaatggcttt aacggcctgg
      181 cctcaaaagt tcagaacgtc ctgaaggatg acccgttctc cggacacctg ttcatcttcc
      241 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
      301 tcaccaaacg cctggagcgg ggccgcttcg tctggccagt cacccgtgac ggcaaggtgc
      361 accttactcc ggctcagtta tccatgcttc ttgaaggtat caactggaag cacccgaaac
      421 gaacggaacg cgctggaatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
      481 cttgctcatg agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
      541 cagatggccg aatataaccg cctgctctca ctgcgggtgg cggcttatgc ttccgaaatc
      601 aaccggctga aggcgctggt tgcaaaactg cagcgcatgc agttcggtaa aagctcagaa
      661 aaacttcgcg caaaaaccga acgacagata caggaagcac aggagcgaat cagcgcactt
      721 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atccgccctg
      781 cgccagtctt cagcccgtaa actgttaccg gcctcacttc cccgtgaaac ccgggtcatc
      841 cggccggaag aggaatgctg tcctgcctgt ggtggtgaac tcagttctct gggatgtgat
      901 gtgtcagagc aactggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
      961 aaactggcct gttgccggtg cgaccatatc gtgcaggcac cagtaccttc aaaacccatt
     1021 gcacgcagtt atgccggagc ggggcttctt gcccatgttg tcaccgggaa atatgcagac
     1081 catctgccgt tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt
     1141 gccacactgg ggcgctggac aggtgctgtt gctgaactgc tggagccact gtatgacgtc
     1201 ctgcgccagt atgtgctgat gcccggtaaa gtccatgccg atgatatccc cgtcccggtc
     1261 caggagccgg gcagcggtaa aacccggaca gcccggctgt gggtctacgt ccgtgatgac
     1321 cgtaacgccg gttcacagat gcctgcggcg gtctggttcg cgtacagccc ggaccggaaa
     1381 ggcatccatc cacaaaatca cctggccggt tacagcggag tgcttcaggc cgatgcttac
     1441 ggtggctacc gggcgttata cgaatccggc agaataacgg aagccgcgtg tatggcccat
     1501 gcccggagaa aaatccacga tgtgcatgca agagcgccaa ccgatatcac cacggaagcc
     1561 ctgcagcgta tcggtgaact gtatgccatc gaggcagaag tccggggatg ttcagcagaa
     1621 cagcgtctgg cagcaagaaa agccagagct gcgccactga tgcagtcact gtatgactgg
     1681 atacagactc agatgaaaac actgtcgcgt cactcggata cggcaaaagc gttcgcatac
     1741 ctgctgaaac agtgggaggc cctgaacgtg tactgcagta atggctgggt ggaaatcgac
     1801 aacaacatcg cagagaacgc cttacgggga gtggccgtag gccggaaaaa ctggctgttc
     1861 gcgggttccg acagcggtgg tgaacatgcg gcggtgttgt actcgctgat cggcacatgc
     1921 cgtctgaaca atgtggagcc agaaaagtgg ctgcgttacg tcattgaaca tatccaggac
     1981 tggccggcaa accgggtac
//
LOCUS       DAJTNP010000243         1953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTNP010000243 DAJTNP010000000
VERSION     DAJTNP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="OO160_004211"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="OO160_004211"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5526077.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>1953
                     /locus_tag="OO160_004212"
     CDS             1649..>1953
                     /locus_tag="OO160_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5526078.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          518 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaa
//
LOCUS       DAJTNP010000244         1929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTNP010000244 DAJTNP010000000
VERSION     DAJTNP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..450
                     /locus_tag="OO160_004213"
     CDS             <1..450
                     /locus_tag="OO160_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526079.1"
                     /translation="ADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYVISWQTL
                     KPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIY
                     KTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            452..1057
                     /locus_tag="OO160_004214"
     CDS             452..1057
                     /locus_tag="OO160_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5526080.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1044..>1929
                     /locus_tag="OO160_004215"
     CDS             1044..>1929
                     /locus_tag="OO160_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526081.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANS"
BASE COUNT          465 a          442 c          539 g          483 t
ORIGIN      
        1 gcagatatcg cggcggccat ctggcgtaag gctccgccgg gtattgatat gaatggcaac
       61 acaacgttca ctgttgcaga taaggagtac gatccgccgt atcctgaata cgtgatcagc
      121 tggcagacac tcaaacccgt cagtctgcat gtcagtgtga cgctgaaaaa aagtgactac
      181 ctgccttcag atattacccg acaggtacag caatccgtgc tggacgcatt taatggtaca
      241 gatggtggtc tgcgggcaag agttgcctct gttgtctctg ccgggcgcta ctatgccggc
      301 atttacaaaa ccgatccgga acatattgat attctgggcc ttactgtgag ccgtgatggt
      361 tcgtcatgga caacggctgt cactttcggg atagatgaga ttccggttct ggatgtgtcg
      421 gatatcagtg tgaaactcca ggaggcataa cgtgcagaat gtggctgcca ctgtgcttgc
      481 gcagtatgct gccagccccc gactcaatgc cctcattaac agctttaacg cagcgctttc
      541 ccccgacagt tttatcagtg atttttatgg tcttatctgg aacatcgata ccgcagaaaa
      601 gtatggtctt gatgtctggg gaaagattgt gggtgtcagt cgctggctga cggtaaagga
      661 cgattttaat tacctgggct tcagcgagtc caggatggac accccggtaa tggatgatcc
      721 ctgtccgttt aatcaggcac cgttttacaa cggaaaatcg gatacccgga ctgttgacct
      781 gtctgatgct gtataccggc ggctgatact gatgaaagcc atgtcgaaca ttactgactg
      841 ttccgttccg gatattaacc tgatgctgag atttatgttc ggaaaaaaac gccgggctta
      901 tgttctgaat aatggcgggc tgaggatgag ttacgtcttt gagtccgcgc tctcgttggc
      961 agaactggcg attatccagt cgtcgggtgc actgccatcc ccgccgggtg tttatgtttc
     1021 agtagtttta aaggagtccc gtaatgaagg ccagtgataa accccgccag ctggcggtcc
     1081 cctttgcgag taccggagat aaaaaccgta tcccggacaa ggcgacacag cagaccagag
     1141 agagcggtaa tgctgcgtat gattcaggtt ttcctccggt gaccatgaca gcggtctcag
     1201 cgggaggtat accgccacac ggcaaggatt ttaacggtct gatgtacgat attaccgcag
     1261 caatacggtt cgcccaggct ggcggtttgt acacgtataa tgccggtttt gcgggggcca
     1321 ttggtggata tgcaaaagga gccattctcg ccggagtcgc aacaacagcg gtctggctga
     1381 ataccacgga cgataacctg accgatcctg aaggctccga cagtgcgggc tgggtaaatc
     1441 ttcttgagga tccgaaaagg atattcctgc ggcagaagaa caatctgtca gaccttcaga
     1501 ataaagggac ggcacgggat aatcttcagg tttacagtaa agagcagtca gatcaacgct
     1561 atgttcatcg ggaaggcgat aaaataaccg gagagctgaa aatccgtggt gttaatgcgc
     1621 tgaggatttt caacgaagct tttggcctga tttttcgtcg ttcggaagag tgcctgcacc
     1681 ttattcccac cagtgaaggt cagggggaaa atggcgatat tggtcccctg cgcccgttca
     1741 ccattaattt gcggacgggt gaaatatcca tgtcgcataa agtgtctgtt ggcggtggtt
     1801 cgcaggtcaa tggtgcgctg ggtatcggcg ttcagaacgc cctggggggg aattcaattg
     1861 ttcttggtga taatgacacc ggatttaaac agaatggaga cggtattctg gatgtttatg
     1921 ctaatagtc
//
LOCUS       DAJTNP010000245         1876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTNP010000245 DAJTNP010000000
VERSION     DAJTNP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(159..1763)
                     /locus_tag="OO160_004216"
     CDS             complement(159..1763)
                     /locus_tag="OO160_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5526082.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          465 a          469 c          425 g          517 t
ORIGIN      
        1 ggtctgtagt aagtgtcatc ctagaagaga aaactttttg tgtcccttct ccctatagtg
       61 agtcgtatta attatccctt gacaagtaga gaaaactatg agtcaaaaac atgaccacac
      121 aggtctgtag taagtgtcat cctagactat ggtctcctct aggtctcctt ataaacatca
      181 gccatcacat tgaccagcct atcaacctcg acctctttag cataacggtc gtacgtaata
      241 gaccctgaac tatgccccat gatcgcctgt gcatacgcca actcgacccc atgttgtttt
      301 aactgggccg caactgtatg tctaaacgag tgaaacgcta agccgctacg gtctacgagg
      361 actttaggca acaacgtccg attaaaccat ttactcacct gctcaccgta accgttcttc
      421 atgagacgca gaccatcgaa caccatagcg tcatcaccgt cagcgcctct acgggtctct
      481 acaagcgcca taaagtcagc caatataaaa ccataggccc catcgaccag aggcacacag
      541 cggtcactat gagcgttctt gatgctctta ccgggtagac tactatcgtc ttcattgata
      601 tgaatataaa cggttcccgc ctcagtggtc ctgacgtctt tgacctgtag ttgggctatt
      661 tcattgagtc tcgccccagt gatagcagcc aaggcggtaa cataataatg atacggctta
      721 ccagaggtct tctgagagta tgccttagct gcgaccagta gttgccccac ctgctccgta
      781 gagaaggcgt tacgggcccc tgaggcttta cgctgaggga ctttaagctc cagaccctca
      841 gtcatgttct ttttaatcaa gtcattgcgc acagcccacc taaagacagc agccatctta
      901 ataagatact tgttgttgat ggtaacgaca tccagacaat ctgtcttatc ttcacggctc
      961 aacaggtcag caagtgggac atccttgaag cgctgcttac ggttcctagg taactgctgg
     1021 agcacatcac ggaccctaag catgtcagcc ctgttagcgt ctgcattgag acccagatgg
     1081 tcgaatattt cgattaacgc agcgtgtgac gccttgtttt cccttaaggt tgcaggcttc
     1141 cagttctgat agttctccgc ttcgtattgt tcaaagaggc tcgctatggt aatgctaggc
     1201 gtaacttctg gttttacttc tggagcatta acagataggg acacagagtc tgcttggtta
     1261 tcttgcctat caatatcatt aaaaagatcg acgtaatcta tcagaggctg actgtctccc
     1321 gcttcgatac gcctcataca ggcttttagc accttcaggg cctcattgat gtaacgatgc
     1381 tggtcaatgc ttagcggctc actagccaga gcatcggtaa ggctctcacc aagttctccg
     1441 tattggtccc ggtagatatc cctcatgtca ggctcaaaga gatctgaacg gcccatagac
     1501 agttcacact cagcgatgtc cttaaggtgt tccctcattt cctcataggt agcgttgggt
     1561 ctgtctgcgt gtattgcctt gagtgctgcc tttatgtgcc ttgagtaagc catagcggtg
     1621 cgtctatcct tggtccgtaa tgatattgag gtcattctat cattgtttga ttgtctgacc
     1681 ctaagacgga agtagtagac gccgttacgc tggtagagat agtagactgc tggaagtttg
     1741 tgactactgg atagctgaat catatgtctg catgccctga gagggttaca cagtgggtta
     1801 cagtaagtaa ctctgcaagg aggcttaaag ccgtatccct taggattcag taggttggag
     1861 tactttaaga aaggtc
//
LOCUS       DAJTNP010000246         1861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTNP010000246 DAJTNP010000000
VERSION     DAJTNP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..259)
                     /locus_tag="OO160_004217"
     CDS             complement(<1..259)
                     /locus_tag="OO160_004217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526083.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1485)
                     /locus_tag="OO160_004218"
                     /pseudo
     CDS             complement(1012..1485)
                     /locus_tag="OO160_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1413..1779)
                     /locus_tag="OO160_004219"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="OO160_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          426 a          519 c          442 g          474 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 g
//
LOCUS       DAJTNP010000247         1816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTNP010000247 DAJTNP010000000
VERSION     DAJTNP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(198..599)
                     /locus_tag="OO160_004220"
                     /pseudo
     CDS             complement(198..599)
                     /locus_tag="OO160_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1028..1816
                     /gene="virF"
                     /locus_tag="OO160_004221"
     CDS             1028..1816
                     /gene="virF"
                     /locus_tag="OO160_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5526084.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          565 a          329 c          337 g          585 t
ORIGIN      
        1 tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac
       61 attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga
      121 gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt
      181 cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag
      241 agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg
      301 ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc
      361 atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc
      421 agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg
      481 gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat gacatcctgc
      541 accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa
      601 tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc
      661 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
      721 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      781 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      841 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      901 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      961 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
     1021 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
     1081 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
     1141 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
     1201 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
     1261 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
     1321 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
     1381 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
     1441 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
     1501 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
     1561 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
     1621 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1681 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1741 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1801 tcataaaaaa ttttaa
//
LOCUS       DAJTNP010000248         1810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTNP010000248 DAJTNP010000000
VERSION     DAJTNP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..234
                     /locus_tag="OO160_004222"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO160_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO160_004223"
     CDS             655..1233
                     /locus_tag="OO160_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526085.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OO160_004224"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OO160_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1810
                     /locus_tag="OO160_004225"
                     /pseudo
     CDS             1584..1810
                     /locus_tag="OO160_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          587 a          322 c          424 g          477 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc
//
LOCUS       DAJTNP010000249         1794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTNP010000249 DAJTNP010000000
VERSION     DAJTNP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            182..529
                     /gene="tnpB"
                     /locus_tag="OO160_004226"
     CDS             182..529
                     /gene="tnpB"
                     /locus_tag="OO160_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526086.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            579..>1794
                     /locus_tag="OO160_004227"
     CDS             579..>1794
                     /locus_tag="OO160_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HCR5526087.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGS"
BASE COUNT          399 a          510 c          534 g          351 t
ORIGIN      
        1 cagagagccc ccagctactt cctgtgactc tcgatgcagc tgccgaacag ccagaatcgc
       61 tcgcagagga cccggaaacc ctcagtatca gctgtgaggt aacgttccgg cacgggacgc
      121 tccgcttcaa tggcaatgtc agcgaaaagc tcctgactct gctgatacag gaactgaagc
      181 gatgatcccg ttaccttccg ggaccaaaat ttggctggtt gccggtatca ccgatatgag
      241 aaatagcttc aacggcctgg ctgcgaaagt acaaacggcg ctgaaagacg atcccatgtc
      301 cggccatatt ttcattttcc ggggccgcag cggcagtcag gttaaactgc tgtggtccac
      361 cggcgacgga ctgtgcctcc tgaccaaacg gctggagcgt gggcgcttcg cctggccgtc
      421 agcccgtgat ggcaaagtgt tccttacgca ggcgcagctg gcgatgctgc tggaaggtat
      481 cgactggcga cagcccaagc ggctgctgac ctccctgacc atgctgtaaa tctctttatc
      541 ctggttgtca cagaataagc ccggtaaaat acgggcttat gaacgacatc tcttctgacg
      601 acatcttcct gctgaaacag cgcctggccg aacaggaagc gctgatccac gccctgcagg
      661 aaaagctgag caaccgggag cgcgaaatag accatctgca ggcgcagctg gataaactcc
      721 gccggatgaa cttcggcagt cgttccgaaa aagtctcccg ccgtatcgca caaatggaag
      781 ccgatctgaa ccggcttcag aaagagagcg atacgctgac tggtagggtg tatgacccgg
      841 cagtacagcg tccgttgcgt cagacccgca cccgtaagcc gttccctgaa tcactacccc
      901 gtgacgaaaa gcgactgttg cctgcggcgc cgtgctgccc gaactgcggc ggttcactga
      961 gctatctggg cgaggatacc gccgaacagc tggagttgat gcgtagcgcc ttccgggtta
     1021 tccggacggt acgggaaaaa catgcctgta ctcagtgcga taccatcgtg caggcacctg
     1081 caccttcgcg gcccatcgag cggggtatcg ccggaccggg gctgctggcc cgcgtgctga
     1141 cctcgaagta tgcagagcac accccgctgt attgccagtc agaaatatac ggccggcaag
     1201 gtgtggagct gagccgttca ctgctgtcgg gctgggtgga tgcatgctgc cggctgctgt
     1261 ctccgctgga agaggcgctt catggctatg tcatgactga cggcaaactc catgccgatg
     1321 ataccccggt ccaggtactg ctgccgggta ataagaagac gaagaccggg cggttgtggg
     1381 cgtatgttcg tgatgaccgc aatgccgggt cagcgttggc acctgcagtg tggttcgctt
     1441 acagcccgga cagaaaaagc atccatccgc agactcatct tgcttgcttc agcggtgtgc
     1501 tgcaagcgga tgcgtacgcc gggttcaacg agctgtatcg caatggtggg ataacggaag
     1561 ctgcctgctg ggctcatgcc cgccgaaaga tccacgatgt gcacgtccgc atcccgtcag
     1621 cactgacgga agaagccctg gagcagatcg gtcagttgta cgccatagag gcggatataa
     1681 ggggaatgcc ggcagagcag cggcttgctg aacgtcagcg aaaaacgaaa ccgctgttga
     1741 aatccctgga aagctggttg cgtgaaaaga tgaagaccct gttcttcggc tctg
//
LOCUS       DAJTNP010000250         1791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTNP010000250 DAJTNP010000000
VERSION     DAJTNP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1..745)
                     /locus_tag="OO160_004228"
                     /pseudo
     CDS             complement(1..745)
                     /locus_tag="OO160_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(796..1371)
                     /locus_tag="OO160_004229"
                     /pseudo
     CDS             complement(796..1371)
                     /locus_tag="OO160_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1375..1775
                     /locus_tag="OO160_004230"
                     /pseudo
     CDS             1375..1775
                     /locus_tag="OO160_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          382 a          512 c          454 g          443 t
ORIGIN      
        1 tgaccgcccg cagggcattt tcagcgatgt tgttgtcgat ttccacccag ccattactgc
       61 agtacacgtt cagggccttc cactgtttca gtaggtatgc gaacgctttt gccgtatccg
      121 agtgacgcga cagtgttttc atctgttgct gtatccggtc atacagtgac tgcatcagtg
      181 gcgcggctct tgcttttctt gccgccagac gctgttctgc tgaacatccc cggacttctg
      241 cctcgatggt atacagttca ccgatacgct gcagggcttc cgtggtgatg tcggttggca
      301 ctcttgcatg cacatcgtgg atttttctcc gggcatgggc catacacgca gcttccgtta
      361 ttctgccgga ttcgtataac gcccggtaac caccgtaagc atctgcctga agcacaccgc
      421 tgtaaccggc taggtgattc tgtggatgga tacctttccg gtccgggctg tacgcgaacc
      481 agaccgccgg gggcatctgt gaaccggcgt tacggtcatc acggacatag acccacagcc
      541 gggcagttct tgttttacca ctgcccggct cctggaccgg aacggggata tcatccgcat
      601 gaactttacc gggcatcagt acatactggc gcaggacgtc atacagcggc tccagcagtt
      661 cagcaacggc accggtccag cgacccagtg tggcacggct cagctccacg ccctgacgac
      721 ggtatatttc tgactggcgg tataactgtc aacgacggat gaaaagtggc tcttccgcaa
      781 cctgggtgga gattttcatg ccagtggact cattaccctc tgcctgagta gttcaaatcc
      841 tgctcgacca tacatctggc gtttcagcat cttcttcagg cgatttacat gaccttcgac
      901 aacaccatta ctccagcgac tactgattgc ctcacatata gctgcagcat cagcttccat
      961 ccccgccgcc acgcgccgaa attctgctga accgctttcg tggactcgag tgaaccaact
     1021 gcttagctgt gatttatttt gggttttcag aatacggtag aactcgagtg ccagttgctg
     1081 cgctattttc agctcaggtt ctttttcaca catcagacta ataaatcggg aagcataatt
     1141 ttcttcatcc ctggttattc tccagggcat caaccagcgg cttacccggg agactgaagg
     1201 aagtctgacg gcagtagtaa ccggtgggtt ccagccttta cgccatttgg caaccgcatc
     1261 cctgacgatg gtttcactgc ctgtaaatcc ccgggccacc atttcccgcc atatccggct
     1321 ggcgttataa ttaccacttt ctcgttgctc ttttaaccac tccctccatg gtgtcagcga
     1381 aaaacgaaac cgctgttgaa atccctggaa agctggttgc ggaaaagatg aagacccagt
     1441 cgcgacactc agagttggcg aaggcgttcg cgtacgcact taaccagtgg ccggcactga
     1501 cgtactatgc gaacgatggc tgggtggaaa tcgacaacaa catcgctgaa aatgccttgc
     1561 gggcggtcag tctgggtcgt aaaaacttcc tgttcttcgg ctctgaccat ggtggtgagc
     1621 ggggagcgct actgtacagc ctgatcggga cgtgcaaact gaatgacgtg gatccagaaa
     1681 gctaccttcg ccatgtgctt ggcgtcatag cagactggcc ggtcaaccgg gtcagcgaac
     1741 tgcttccgtg gcgcatagca ctgccagctg aataacacat ccccgtcaat a
//
LOCUS       DAJTNP010000251         1782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTNP010000251 DAJTNP010000000
VERSION     DAJTNP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            173..1198
                     /locus_tag="OO160_004231"
     CDS             173..1198
                     /locus_tag="OO160_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5526088.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1782
                     /locus_tag="OO160_004232"
                     /pseudo
     CDS             1266..1782
                     /locus_tag="OO160_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          416 a          417 c          453 g          496 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cg
//
LOCUS       DAJTNP010000252         1769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTNP010000252 DAJTNP010000000
VERSION     DAJTNP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..360
                     /locus_tag="OO160_004233"
     CDS             <1..360
                     /locus_tag="OO160_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5526089.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO160_004234"
     CDS             361..726
                     /locus_tag="OO160_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5526090.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO160_004235"
     CDS             723..1406
                     /locus_tag="OO160_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5526091.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="OO160_004236"
     tRNA            1605..1680
                     /locus_tag="OO160_004236"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="OO160_004237"
     tRNA            1682..1758
                     /locus_tag="OO160_004237"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          488 a          374 c          488 g          419 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttg
//
LOCUS       DAJTNP010000253         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTNP010000253 DAJTNP010000000
VERSION     DAJTNP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(110..577)
                     /locus_tag="OO160_004238"
     CDS             complement(110..577)
                     /locus_tag="OO160_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5526092.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO160_004239"
     CDS             complement(643..882)
                     /locus_tag="OO160_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5526093.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO160_004240"
     CDS             complement(1017..1622)
                     /locus_tag="OO160_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5526094.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTNP010000254         1654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTNP010000254 DAJTNP010000000
VERSION     DAJTNP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            51..266
                     /locus_tag="OO160_004241"
     CDS             51..266
                     /locus_tag="OO160_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5526095.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            270..899
                     /locus_tag="OO160_004242"
     CDS             270..899
                     /locus_tag="OO160_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526096.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            965..1498
                     /locus_tag="OO160_004243"
     CDS             965..1498
                     /locus_tag="OO160_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5526097.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1495..>1654
                     /locus_tag="OO160_004244"
     CDS             1495..>1654
                     /locus_tag="OO160_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5526098.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITD"
BASE COUNT          442 a          359 c          448 g          405 t
ORIGIN      
        1 tcatttctca gtgtaaaact acgccatccg ttatttgcgg aggtgaggct atgaaatcca
       61 tggacaaaat ttcaacgggc attgcctacg gcacctccgc cggcagtgcc ggctactggt
      121 ttttgcagtg gttggatcag gtcaggccat cacagtgggc tgcgattggt gtgctgggaa
      181 gtctgcttct ggggcttctg acttatctga cgaatctgta tttcaaaata agagaagaca
      241 agcgtaaggc tgcacgggga gagtaaataa tgaagcatga agaaatgaat cagcgattca
      301 atcacctgga aaatgaaatc actgagctta ataaaaaatt gtcggcgttg gtgagttctg
      361 aagatgaaaa caaacgccgc gatgagcatt atgcggcgat ttacgattac tgccacaaag
      421 tcgctcacga gacttttatg aagtttttgc aggaaaagtt tttaccggcc gcattgtcag
      481 aaaaagaggc ggcttacctg cggcctgagt acgtcattac ggttaacagt gccggagagg
      541 aagagcataa aagtgatttt attgcgtctg caccggataa agaccaggaa ccccatcggc
      601 ctttcagggt ttcctgtgaa gagggtgagt tcgtcgttta tgagaacgga aaacctgtcc
      661 gggcatcgca tcatcactgc ctgaaaatta ttaatctcgc tatcagatgc ctgaaagacg
      721 aaaacaccag agttatgaaa cgcattggcc gttgcatggg gtatttgcag gtggcggcag
      781 agattgaagc acttgccagt ggtgcagaca tggatgctgc ggtgcgggag gctcttcttc
      841 gtgatttcaa tactccccct ttaagaaaga gcctgatgac tggatccagc cggggctgac
      901 ttatcttaaa aggcgtatat aagttggctc gttatttgtt gccgataaat cctgataaat
      961 atccatgagc gcaaaaatca aatacggcct gtcggctgct gttctggcgc tgattgccgc
     1021 aggcgcgtct gctccccaga tacttgacca gtttctgaac gaaaaagagg gtaatcacac
     1081 aacggcatac cgtgatggtt ctggcatatg gaccatctgt cgtggtgcca caatggtgga
     1141 tggtaagcct gtcataccgg gaatgaagct gtcgaaggaa aaatgtgacc aggttaacgc
     1201 tattgaacgt gataaggcgc tggcatgggt ggagcgcaat attaaagtac ctctgaccga
     1261 accccagaaa gcgggtatag cgtcattttg cccctataac attggccccg gtaagtgttt
     1321 cccgtcgacg ttttataagc ggctgaatgc cggtgatcgt aagggcgcat gcgaggcgat
     1381 tcgctggtgg ataaaagatg ttgggcgcga ttgccgcata cgttcaaata actgctatgg
     1441 acaggttatt cgtcgtgacc aggaaagcgc attagcctgt tgggggatag atcaatgagc
     1501 agagtcaccg cgattatctc cgctctggtt atctgcatca tcgtctgcct gtcatgggct
     1561 gttaatcatt accgtgataa cgccattacc tacaaagagc agcgcgacaa aaacgccgga
     1621 gaactgaagc tggcgaacgc aaccattact gaca
//
LOCUS       DAJTNP010000255         1631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTNP010000255 DAJTNP010000000
VERSION     DAJTNP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..166
                     /locus_tag="OO160_004245"
     CDS             <1..166
                     /locus_tag="OO160_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526099.1"
                     /translation="QLLQARQREFATKWGRAALPHERLTYFPLEDLIKNNGKKIRSDK
                     KHFLECNEDK"
     gene            complement(757..900)
                     /locus_tag="OO160_004246"
     CDS             complement(757..900)
                     /locus_tag="OO160_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526100.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            1139..>1631
                     /locus_tag="OO160_004247"
     CDS             1139..>1631
                     /locus_tag="OO160_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5526101.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVG"
BASE COUNT          516 a          343 c          340 g          432 t
ORIGIN      
        1 gcaactgtta caggcacgcc aacgggagtt tgccactaaa tggggacgtg ccgcgctgcc
       61 acatgaacgc ctaacctatt ttccacttga agacctcatt aaaaataatg gcaaaaaaat
      121 caggtctgat aagaaacact tcttagaatg caatgaagac aaataacaac aatctgcata
      181 ttgccaccaa ccacagatgt ggcatcgtta tcagcgcact tgattttctt tatattcact
      241 gacgagcttg ccggggcacc tttgtttgcc ggtcattctg gtatcatgca gatatttacg
      301 gcgtacaagc ccactagcat gacattgtaa taaggcctga gcctaacgct ttaatgtgtg
      361 tcatacactc agtggcgtaa tcgttgacaa tatggtgcat gtaatcgtaa gcgtccagcg
      421 aaatccatct ggtcataatc tgaagcatcc gacaaagtgg tgtccaccaa ataagtgatc
      481 tgcgcccaat atcctgagcc ggaactttga caaaggccaa cgaaaaatgg gagcggatgt
      541 tacagagttc tcggtacagg ggaaaagtgg tacctgtcgc cagttctccg atctgtttaa
      601 ccgggaaatt atctcctaca gcttgtcaga aaggtcggtg atggagcatc gttaatacga
      661 tgctatacga tgcattcaca gtgcccggcc agaggatgcc ccgtcgctgc atatggatca
      721 gggttggcaa tatcgaatcg caggctatag gcaaagttaa agccccatgg agtagcacaa
      781 aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tggagaactt cttcggcaca
      841 ctgcaaaatc gcatcgtggt cgatcgctaa ttcgtcgtga atcactgaac gatgttccat
      901 gcgaacagat attcgatcat atcgaaatat taaacattac ccctccccac attgggcaaa
      961 aaaaaacaaa aaacgatcaa ttagtattga ttcacataat ctctcatcat taatgagaac
     1021 attgtcacat atttacaata tagaactgtt acttttagca agctgttagt aaccaaccgt
     1081 tactaataat tttaattctt aatactttgt tcattcaata aatgagttga gagaatatat
     1141 gaataaaata tactccctta aatatagtgc tgccactggc ggactcattg ctgtttctga
     1201 attagcgaaa agagtttctg gtaaaacaaa ccgaaaactt gtagcaacaa tgttgtctct
     1261 ggctgttgca ggtacagtaa atgcagcaaa tattgatata tcaaatgtat gggcgagaga
     1321 ctatcttgat cttgcacaaa ataaaggtat tttccagccc ggagcaacag acgtaacaat
     1381 cactttaaaa aacggagata aattctcttt ccataatctc tcaattccgg atttttctgg
     1441 tgcagcagcg agtggcgcag ctaccgcaat aggaggttct tatagtgtta ctgttgcaca
     1501 taacaaaaag aaccctcagg ccgcagaaac tcaggtttac gctcagtctt cttacaaggt
     1561 tgttgacaga agaaattcca atgattttga gattcagagg ttaaataaat ttgttgtgga
     1621 aacagtaggt g
//
LOCUS       DAJTNP010000256         1621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTNP010000256 DAJTNP010000000
VERSION     DAJTNP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..374)
                     /gene="arsC"
                     /locus_tag="OO160_004248"
     CDS             complement(<1..374)
                     /gene="arsC"
                     /locus_tag="OO160_004248"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5526102.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFS"
     gene            complement(387..>1621)
                     /gene="arsB"
                     /locus_tag="OO160_004249"
     CDS             complement(387..>1621)
                     /gene="arsB"
                     /locus_tag="OO160_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5526103.1"
                     /translation="KGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAV
                     IIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPI
                     VIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYASVM
                     VPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVG
                     FFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYG
                     LRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTA
                     SGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTL
                     PVLFVTLAALALRLSFTL"
BASE COUNT          436 a          457 c          420 g          308 t
ORIGIN      
        1 gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga agggcggcac
       61 aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg gtgctgaagc
      121 ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag ctcctcatac
      181 ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc aatgagtttg
      241 accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg ttctgtgccg
      301 ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt gtgataaatg
      361 gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag cgccagcgca
      421 gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa gtaatatccc
      481 cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagcagcgt tgccaggcta
      541 ccaatcgggg taattttcgg ccccaaatcg cagccaatca cgttggcata aaccatcgct
      601 tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc
      661 ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc gagcgtcgcg
      721 gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc cgttaatccg
      781 gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac aatctgccag
      841 ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc gacgacaaat
      901 aatatcagtg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg
      961 aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt agcaggatct
     1021 ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg aatatctttg
     1081 cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc caccggcacc
     1141 atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac gatattcacc
     1201 aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc cgccatcacg
     1261 aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat gacaatcggc
     1321 gtcaaaataa gcgccgcgcc atcattggca aacagggccg caacggcggc accgagcagg
     1381 acaatccagg taaacagcaa gcgaccgcgt ccattgcccc agcgtgaaac gtgcagcgct
     1441 gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac ggcaataaac
     1501 gccgccgtcg cgttccagac gatattccac accaccggaa tatcacccgg atgaaccacg
     1561 cccgtaacca acgccagtac cgcgccgagc gttgcactcc agccgatgcc taaacctttc
     1621 g
//
LOCUS       DAJTNP010000257         1604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTNP010000257 DAJTNP010000000
VERSION     DAJTNP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            240..509
                     /locus_tag="OO160_004250"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO160_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO160_004251"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO160_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO160_004252"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO160_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5526104.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          477 a          330 c          377 g          420 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaa
//
LOCUS       DAJTNP010000258         1561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTNP010000258 DAJTNP010000000
VERSION     DAJTNP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            400..1149
                     /locus_tag="OO160_004253"
     CDS             400..1149
                     /locus_tag="OO160_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5526105.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1561)
                     /locus_tag="OO160_004254"
     CDS             complement(1319..>1561)
                     /locus_tag="OO160_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526106.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          427 a          332 c          315 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAJTNP010000259         1461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTNP010000259 DAJTNP010000000
VERSION     DAJTNP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            187..438
                     /locus_tag="OO160_004255"
     CDS             187..438
                     /locus_tag="OO160_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526107.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            505..783
                     /locus_tag="OO160_004256"
                     /pseudo
     CDS             505..783
                     /locus_tag="OO160_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            785..>1461
                     /locus_tag="OO160_004257"
     CDS             785..>1461
                     /locus_tag="OO160_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5526108.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          357 a          368 c          385 g          351 t
ORIGIN      
        1 ctctgatgtg gcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc gggtttttgt
       61 ttttattttc aacgcatttg aagttctgga tggtgccgga atagaatcaa aaatacttaa
      121 gtagcgcgca gggataaaag ggatggaccc cgaacagggg agtgctactt atctggaagg
      181 attctgatga tgaacatcga agaactgcgt aaaatttttt gtgaagatgg cctctatgct
      241 gtgtgcgttg aaaatggaaa tattgttagt cattaccgca ttgtgtgttt gcaaaaaaat
      301 ggggctgcgt taattaattt tgtggatgcc cgagtgacgg acggatttat cttgcgcgac
      361 ggtgagtttg tcacttcatt acaggcattg aaagagatcg gaataaaagc tggcttttct
      421 gctttttcag aagaataaac tcatctacaa tcttgcgcgg ggctgaactc ccgctgagta
      481 acaccgtgcc accggagaaa accgatggca cgcaacgcaa aatattacaa tcatgataat
      541 tcgaccgttc ttgcccacac gcacgagcgg tattctcacg catttaagtc agactggtac
      601 cagcatccct catgcactga agaacaggcc gaatggctta ttcagtgtta ccgcaggcgc
      661 ggatgcgagg ttaaaaaagc ccttagcctc gactaccgtc actagataat ctccgtcagg
      721 ctcccttact ccgaacagcc accgcgtccg tcccgcacat tccagcaacg gatctggagg
      781 taacgtgcgg gtattacttc gacctgttct ggttccggaa ctcgggctgg tggtccttaa
      841 gcccggtcgt gaatccatgc cggtattcca caatacccgg gtactggtgg agccggaacc
      901 gaaaagcatg cgtaatctgc cgtccggggt cgttcctgcc gttcgccagc cgctagtgga
      961 agacaaaaca ttgctgccgt ttttcagtaa cgcacgggtg attcgtgctg ctggtggtgc
     1021 tggcgcattg tctgactggc tgttgcgcca tattaaatcc tgccagtggc cacacggcga
     1081 ttatcatcac agcgaaaccg tcattcaccg ttatggtacc ggcgcaatgg tattgtgctg
     1141 gcactgcgac aaccagctgc gtgaccagac atccgaatca ctcgagcaac ttgctcatca
     1201 aaacctgtca gcatggatga ttgacgtcat cggtcacgca ataagcggta cgcaggagcg
     1261 tgaattatct ctggctgaat tatcctggtg ggcggtccgc aatcaggtgg cggacgcgct
     1321 accggaagcg gtattacgtc gttcgctggg gttgcgtgcg gaaaaaatcc gctcaatgta
     1381 ccgtgaaagc gacatcgtgc cgggagagca gaccgccacc agcatactga agcagcgcac
     1441 aaaaaatctt gcgccgctgc c
//
LOCUS       DAJTNP010000260         1443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTNP010000260 DAJTNP010000000
VERSION     DAJTNP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            82..1275
                     /locus_tag="OO160_004258"
     CDS             82..1275
                     /locus_tag="OO160_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5526109.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="OO160_004259"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="OO160_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAJTNP010000261         1426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTNP010000261 DAJTNP010000000
VERSION     DAJTNP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1..265)
                     /locus_tag="OO160_004260"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OO160_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO160_004261"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO160_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5526110.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1171..1426)
                     /locus_tag="OO160_004262"
                     /pseudo
     CDS             complement(1171..1426)
                     /locus_tag="OO160_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          409 a          354 c          245 g          418 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca ggactaacag
     1201 ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt
     1261 agtgttgtac gaataccaga gccagtgttg ttgcagcctt gctgccaacg aagcgccact
     1321 gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca gatgttatcc
     1381 gccggggcgg gagcttttta atgtccgtat gacggtattg atgccg
//
LOCUS       DAJTNP010000262         1406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTNP010000262 DAJTNP010000000
VERSION     DAJTNP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            144..287
                     /locus_tag="OO160_004263"
                     /pseudo
     CDS             144..287
                     /locus_tag="OO160_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            434..700
                     /gene="ospE1"
                     /locus_tag="OO160_004264"
     CDS             434..700
                     /gene="ospE1"
                     /locus_tag="OO160_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR5526111.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1091..>1406
                     /locus_tag="OO160_004265"
     CDS             1091..>1406
                     /locus_tag="OO160_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5526112.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTH"
BASE COUNT          495 a          290 c          230 g          391 t
ORIGIN      
        1 catcccgtat tgggcagtat gatgccggaa agctgcgagg ctccacacct gccagtttca
       61 cccttttaac catcatacta ctccgtgttt aattgtgtgg gatgcgttca ttacatttta
      121 acagtgtcac cccgtcggcc acgaaagata aacaggtgac cagaaaaggg atcttcatcc
      181 agcacatgct atatcttgaa cgtaacaacc tgaccctccg cacccgcatc aagcggctgg
      241 cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca tcggctcctt
      301 cattgaaaaa cacatattct actgattgga agcgccaccc gaaaaagtat cgttttttta
      361 cagtaaactt catttagccg actataatgt aaaaaaataa attttttacc aaaatataac
      421 ataaggaagt aatatgctta cacaaactat attcccttgt cttccacaaa aacaagaaaa
      481 tatcatcctt gaagtatcaa atcctgtatt attatcttct actgttacta cagatggata
      541 tactgttttc aataaaaaag cagctatata cgaattacaa atacctgctg ccagccggac
      601 gaaaacgttg aaattcactg ctacagaaat gcaatggttg acaaagataa atgaagccgg
      661 aattgatgaa aaacaaagcc aacggtattc tgatttctga agttaatttc agactcgttt
      721 aagctccatt ttcaaaaaaa atatatcagg gtttgttact aataacttct gaaaacagaa
      781 aaaaaataga gcagtaattc tatttcagag aggagtaact ccatctatcc tcgatacgta
      841 ctgaacgtta tcgtgatata ttcatcatgc tttcttcacc attattcgag tatagggaga
      901 gacgcaatga ccttaaatac ctttttatgg ctgaattacc attctcagga ataacagcag
      961 actccttccc atagacggct tagggggaaa gcaatacccg gagaaagggt acaaagcaaa
     1021 ctgttttaaa attaataagc catattctat ttaactagaa ttcttatcgt tgataagaat
     1081 tctagttata atgattaaat caaccaatat acaggcaatc ggttctggca ttatgcatca
     1141 aataaacaat gtatactcgt taactccatt atctttacct atggaactga ctccatcttg
     1201 taatgaattt tatttaaaaa cctggagcga atgggaaaag aacggtaccc caggcgagca
     1261 acgcaatatc gccttcaata ggctgaaaat atgtttacaa aatcaagagg cagaattaaa
     1321 tttatctgag ttagatttaa aaacattacc agatttaccg cctcagataa caacactgga
     1381 aataagaaaa aacctattaa cacatc
//
LOCUS       DAJTNP010000263         1304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTNP010000263 DAJTNP010000000
VERSION     DAJTNP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            51..971
                     /locus_tag="OO160_004266"
     CDS             51..971
                     /locus_tag="OO160_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5526113.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            971..1304
                     /locus_tag="OO160_004267"
                     /pseudo
     CDS             971..1304
                     /locus_tag="OO160_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          362 a          300 c          324 g          318 t
ORIGIN      
        1 agtgatatca ccaaccgtca aaacgaaccg acaactccat gaggaactga atgatgaata
       61 ataaagtcag cttcactaac agcaataatc caaccatctc tttgtctgca gtgatctatt
      121 tccctccaaa atttgatgaa acccgtcagt atccggcaat tgtggtatct cacccaggtg
      181 ggggcgttaa agagcaaacg gccggaacct atgccgaaaa gctggcggaa aagggatttg
      241 tcactattgc ttatgacgca tcttatcaag gtgaaagtgg cggcgagccg cgtcagttag
      301 aaaacccata cattcgtact gaagacgtca gtgccgtgat tgattacctg acaacgcttt
      361 cttatgtcga caatacccga attggtgcga tgggaatttg tgccggtgca ggatatacag
      421 ccaatgctgc aattcaggat cgccgcatca aggccatcgg taccgttagt gcggtcaata
      481 ttggttcgat gttccgcaat ggttgggaaa acaatgtgaa atctattgat gctctgccac
      541 acattgaagc tggctcaaac gcccgaacca gcgatattag cagcgaagaa tacgccatta
      601 tgccactggc tccaatgaag gaggccgatg cgccgaacga agaactgcgt caggcctggg
      661 agtattacca cacacctcgt gcgcagtatc caacagcacc gggttacgct actttgcgca
      721 gccttaacca gattattacc tatgatgctt accatatggc ggaagtgtac ctgactcagc
      781 cgatgcagat tgtggcgggc agccaggcag ggagcaaatg gatgagtgac gacctgtacg
      841 atcgggcatc aagccaggat aaacgctatc acattgttga aggcgcaaac catatggatc
      901 tttatgacgg caaagcctat gtggctgaag caatttcagt attagctccg ttctttgagg
      961 aaactctgta atgaaacata ttcaaatccg caatagtgat atggcctggc atattgctgc
     1021 caatattcag tttccaccaa attttgatga gtcaaaacag tatccggcga ttatcagcgt
     1081 ccatcctttt ggcagttgta aggagcagac gtctggcaat atctatggca aggcattggc
     1141 tgaaaaaggc tatgtcgttc tcgcttatga cgccagtttc cagggagaat ctggtgggta
     1201 gcctcgttgg atagaagatc cgacacagcg cgtagaggat attagccgtg ttatcgatta
     1261 tgcagtaact ctgccatatg ttgatgctga acgcattggc gtgc
//
LOCUS       DAJTNP010000264         1288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTNP010000264 DAJTNP010000000
VERSION     DAJTNP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            522..806
                     /locus_tag="OO160_004268"
     CDS             522..806
                     /locus_tag="OO160_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5526114.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="OO160_004269"
     CDS             806..1081
                     /locus_tag="OO160_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5526115.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          322 c          325 g          302 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag gacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc cagacatt
//
LOCUS       DAJTNP010000265         1241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTNP010000265 DAJTNP010000000
VERSION     DAJTNP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..1079)
                     /gene="treF"
                     /locus_tag="OO160_004270"
     CDS             complement(<1..1079)
                     /gene="treF"
                     /locus_tag="OO160_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR5526116.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLE"
BASE COUNT          307 a          314 c          327 g          293 t
ORIGIN      
        1 ctctccagtt taaacaggaa ggcattcaga tcgatgggga tgaactgggt ggtacgaatg
       61 ctcgccagac gaccagtatc acgcagccaa cgggaagagt aatcccaacc ggaggccgct
      121 cccgcgcgta aatcgcggta cacctcgttg ggcgggcgac cagaatgttt cgcggtttca
      181 acgtcctcaa gccaggattc gtcacgcggc gtgtcgcgat cgtcccagta acggttgagc
      241 agcgatccgt ccggcatccg cacaacatgg cgataggcct gattagggat caacgattct
      301 gcaccgtcca tccagaaggc atattccatt ttaaggtggt cgagatagcg gcgcgcaccg
      361 cgtacaccat cttcttcaaa caactccacc atcagcgcaa aaaccggtgg ttgcgagcgg
      421 ctcaaataat aggtgcggtt gccgttgggg atgtgaccgt agttttcgat catccaggcg
      481 aagttatcgg ccatgcattt cagcaaatct tcccgaccac tttccgccag tcccagcatg
      541 gtgaaatagg aatcccaata gtaggtttcg ctaaaacggc cgcccgggac aatatatgac
      601 tgcggtagcg ccagcagaga agaccacgga atgtgatcct gtggttcgcg ggttagcacc
      661 ggccacagct ggtcgatatg ctctttcagg gaattttgcg ggtccgatac atactcgctg
      721 gagtagacct ccggcagcca gaagtgattt tcaacaaact tgcgcaagtc aaaatcacga
      781 tgacggcgca ctttacggta gcggattaaa atatccagcg ggtccatttt gggtgcgcag
      841 tcgggaaagg ttttactgtc ggggaaaatt ttcgccgact gaacatgctc gaacagttcg
      901 agatagcgat cggcaggcgt cagcgcatca gaggcgggca gcccttcaat catctcgggt
      961 tccggttctg cctcgatcat ctcatccagt tttaattcat acgggtccag ttcatagcag
     1021 agatcgacct cgatcatcag ttcgtctgga ttagggtttt gaattttctg attgagcata
     1081 aggatccaac ctccgaaatt cgtcgctaat tatcgggctt ttctttaagg gtagtcattc
     1141 gctccgcctg gtgggcgaca aagcgtgcgg tggatcacgt ttattaactt gtcaaaaaat
     1201 ataacgcact aatatcaatg gaatttataa aaatacggat t
//
LOCUS       DAJTNP010000266         1233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTNP010000266 DAJTNP010000000
VERSION     DAJTNP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            188..>1233
                     /locus_tag="OO160_004271"
     CDS             188..>1233
                     /locus_tag="OO160_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5526117.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          401 a          282 c          195 g          355 t
ORIGIN      
        1 ctctgcattg tgaatatgta aaacatcaaa aaaagatgaa ccaaatatac gaaaaaagca
       61 aatatactta ccagggtaga aagcatgata taacactcat tcccttatgg caaatattct
      121 gaaacaccct cctcattagg agagatacat taaatacaat ttattcacaa tctaattcca
      181 tatccatatg cgagaaatta atatgctcag aaacatttca tcctgtttat ttccacatat
      241 cagcacaatt acatccccca accattattt gtccgaatgg gatgattggg agaaacaggg
      301 gttaccggaa gaacagcgta ctgaggcggt aagaagactt cgtgcatgtc ttacctctaa
      361 ggggcataaa ctggacctgc gagccttggc gctttcctcg ctacctgtac tccctgcttg
      421 cattaaaaag cttgatgtga gctgtaataa attaaccatc cttactgatt tacctgaaaa
      481 tattaaagaa cttattgcaa gagataattt cttaacacat atatctgcat taccacatta
      541 tctaataact ttggatgtgt ccgaaaatca attagagaat ctgccgttat taccagacac
      601 catcaaatca ctaagcgcag agtataatag gttatccaca ctgccttcat tacccttgaa
      661 tttaaaaaaa cttgaggtta ggaacaacga actgcaaact cttccatctc tgccttctaa
      721 tcttaagata cttaaggttg cgcacaacca tcttactgaa ctgccccctt tacctaggag
      781 actgcaactt ctttttgcat atagcaatag attaagcaac ttaccaaaca tccaagaaaa
      841 tattatcatg agaagatttt tttattttga aaacaaccaa ataactacaa tcccgacaaa
      901 tctttttcgt ttagatcctc atataactat tgagattgca aataacccct tatcagatca
      961 aactctgcta ttcttaatac agcaaacttc ggttccaaat tttaacgggc ctcagtttcg
     1021 tatttccctg tcagaccaaa acagactgtt tttacgccag atgttgccgc aaaatttaca
     1081 ttcgcgccat atcagagtca tcactgaagg ggggcagaac tttcagatcc cccctcttcc
     1141 cgaaactgtg gcagcctggt ttcctgaagc agatcgtcgg gaggtttcta cacaatggac
     1201 ttctttttcc accgaggaga attcccgggc att
//
LOCUS       DAJTNP010000267         1212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTNP010000267 DAJTNP010000000
VERSION     DAJTNP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(267..1175)
                     /locus_tag="OO160_004272"
     CDS             complement(267..1175)
                     /locus_tag="OO160_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526118.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          234 a          330 c          293 g          355 t
ORIGIN      
        1 caatcagtat gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc ccttcctcgg
       61 gaggggcttt cccgtttcag ccctttgctg actccccgat tgttgatgag cgccggacct
      121 cgcgcaacgc tttcccagag gcagttttgc cttccccgag cggtcacgca aaacgttgca
      181 acgacctgat catttgcagt ttttagcgga acgcagatat aaaaaatccc cggctttttg
      241 tgccagggac gttcatagct gaaaatttat tttacgtttt cggcgacggg agttgttgag
      301 attaagggtg aagtcaatgc cgccagtgaa ccgcctgaaa cgccaatttc attgagcaag
      361 gagtcaatca gcggagcctg tgtgcggtaa gagagagcgg ctgataatgc ttgttccgct
      421 aggtttccgc cccccacatt acccgtgttt gcatcacccg cagcgctgcc actattcaga
      481 ccatcgacct gaataatctt gataccgtca atagatttca tcggctcaac ggatttttct
      541 attaccgcag gtagcgcctg caacaaggcc agtttgaatt taaggctggt ttgttcatca
      601 gaaagtacgt tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc cagacccttt
      661 ttatgtgtag cttcggctaa ctcaacgata gccgccgcct gcatctctgc ggcttctttt
      721 tctgctttcg cccgcacggt cagttcaacc gctttggttt ctgcatcctg cgcggcagcg
      781 attagggcaa cttgtttagc acgatcggct tctgcggtct ggcgagtggt ttctacgttt
      841 tgttgcgcac ttactgcttc tgcgagtgca aggttagcac gcgcttctgc ctgggactgt
      901 tgttccgatt tggcggcaat agcgatcgat ttcgtctggt tggcgatttc ggtgacctgc
      961 tgctgttcga tctctttaat acgaacttca cgttcagcct caacctttct tgagcggacg
     1021 gcctgctcgc gctcgatctc cgtttcctga atttgtcgtt cagccagaat tcgcgtctgc
     1081 tctgcttcac gacgacgttc agcttcaaaa gccgcaatac gtgcattctg ttcggcagta
     1141 cgggttttaa cctgctgctc ctgctcaagc gtcataaacg cttcttgttg ttcggtaatg
     1201 actccaactt ac
//
LOCUS       DAJTNP010000268         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTNP010000268 DAJTNP010000000
VERSION     DAJTNP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..312
                     /locus_tag="OO160_004273"
     CDS             <1..312
                     /locus_tag="OO160_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5526119.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
     gene            315..>1190
                     /locus_tag="OO160_004274"
     CDS             315..>1190
                     /locus_tag="OO160_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5526120.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLI"
BASE COUNT          207 a          322 c          321 g          340 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg
      301 caaggagctt aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta
      361 tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg
      421 acatgtttga tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc
      481 tcctggcgat tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgttaa
      541 aacgcattgt tgacctgccg atgaaactgg tggggcataa accaggcggg ctgggctacg
      601 tcggcgtgct ggcagctatg attatggcga gcctttctgg ttctgccgtt gcggataccg
      661 ccgccgttgc cgctctactg gtgccgatga tgcgcagcgc caactacccg gtcaaccggg
      721 cggcggggct gattgcctct ggcggcatta tcgcgccaat tattccccct tccattccgt
      781 ttattatctt cggtgtttcc agcggattat ccatcagtaa gctgtttatg gcgggcattg
      841 cccctggcat aatgatgggc gcaacgctga tgcttacctg gtggcggcag gccagtcgcc
      901 ttaatttacc ccgccagcaa aaagcaacga tgcaggaaat ctggcactct tttgtctccg
      961 gtatctgggc gctgtttctt ccagtcatca ttattggcgg gtttcgctcc ggtctgttta
     1021 cccctaccga agccggggcc gttgctgctt tctatgcgct gtttgtcgcc acagttattt
     1081 accgtgaaat gaccttcgcc acactttggc atgtactcat tggcgcggcg aaaaccacct
     1141 cagtggttat gtttctggtg gcctcggcac aagtttccgc ctggctgatt
//
LOCUS       DAJTNP010000269         1189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTNP010000269 DAJTNP010000000
VERSION     DAJTNP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..503)
                     /locus_tag="OO160_004275"
     CDS             complement(<1..503)
                     /locus_tag="OO160_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR5526121.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLL"
     gene            600..>1189
                     /locus_tag="OO160_004276"
     CDS             600..>1189
                     /locus_tag="OO160_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5526122.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEV"
BASE COUNT          346 a          237 c          291 g          315 t
ORIGIN      
        1 agcaaattct cacaaagctg ctgtagccgc tcatcatttg gccaagggag ttcaagtgac
       61 attttcaccc ccatttttat ttcctgaagt atcagtgtct cgagggctga aaaacgtaac
      121 tgttgtttat ctgtggtact ttgcgacggt ggaagttggt taattgcaat tagtaattcc
      181 cttaacaggg gtgaaatacc cgtcatgaag caaactgttc ccagcgtttc actttccgct
      241 tctgaaacca gtacggtttt taaaagtacg ttgcctgtcg caacaaactt atgcggcatt
      301 tgtgggggca gccaaagcgc gctgctggca tggactagcc agacgctttc tccagcccat
      361 acacgaacga ctccttcagc cgggtaaagc aactgggcat agagatgttt atgccaggga
      421 actgtacctc cattcagatg ttccgaatcc agtccgataa tgtgatgccc tggtgatgct
      481 ttgggttgca gtgtaattgt catttacagt aggtattttg tcagaaatca tcaaataagc
      541 catgttaaat tttaaatatg acgtatgcaa gtttccggat atatacggtg taaagagtga
      601 tgataatttc taaaaaacag cttattgggg tggtagcgat cggtattctt ttgactggcg
      661 ttgtcttttt tatctggagg gtaagcaaag gaagatttat acagacgacg gatgatgctt
      721 acattggtgg taatatcacc acggtggcaa gtaaagtttc tggttatatt tctgctatag
      781 aggtgcgcga taatcagagc gtaaaaaaag gcgatattat cctgcgtctc gatgatcggg
      841 attatcgtgc aaatgtcgcc aggcttgagg caaaaataaa aagtagcaaa gccaatctcg
      901 aaggcattca agccactatt acgatgcagc agtcgataat tcaaagtgcc agtgaaacat
      961 ggcaggcagt aaaacatgag gagcaaaagc gcctgcgaga tactgaacgt tatgaaaaac
     1021 tcgcacaatc agcggcaata tcgcaacaaa ttattgataa cgccaggttt gactaccagc
     1081 aggtcgcagc aaaggagcgc aaagccgcga acgatttcct ggtggagaag caacggctgg
     1141 cagttttatc tgctcaggaa gaaaacgtac gtgcgagtat cgaagaagt
//
LOCUS       DAJTNP010000270         1186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTNP010000270 DAJTNP010000000
VERSION     DAJTNP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            38..778
                     /locus_tag="OO160_004277"
     CDS             38..778
                     /locus_tag="OO160_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5526123.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..>1186
                     /locus_tag="OO160_004278"
     CDS             837..>1186
                     /locus_tag="OO160_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5526124.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAG"
BASE COUNT          284 a          273 c          370 g          259 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cgggga
//
LOCUS       DAJTNP010000271         1179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTNP010000271 DAJTNP010000000
VERSION     DAJTNP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..1048
                     /locus_tag="OO160_004279"
     CDS             <1..1048
                     /locus_tag="OO160_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5526125.1"
                     /translation="GRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKA
                     ANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTT
                     YGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHR
                     LTPIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQ
                     LIEEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDL
                     WVPNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEY
                     LPVARLEDGTLLNW"
BASE COUNT          281 a          268 c          326 g          304 t
ORIGIN      
        1 tggccgcgat gccagccgta tcgaagatat ctggcaattc ttttataaag gtgcttactg
       61 gcgacgtggc cccgtcacga tgtcagccat ttctgccatt gatatggcgt tgtgggatat
      121 caaagctaaa gcggcaaata tgccattgta tcaactgctt ggtggtgctt ctcgtgaagg
      181 cgtgatggtt tattgccaca ccaccgggcg tacgattgat gaagtccttg aagactatgc
      241 aaagcaccag cagatgggct ttaaggcgat tcgtgtgcag tgcggtgtac cgggtatgca
      301 aaccacttat ggcctggcga agggcaaagg actggcgtat gaaccggcaa ccaaaggcct
      361 gtggccagaa gagcagttgt ggtcttcaga aaagtatctc gattttacgc cgaagttatt
      421 cgaagcggtg cgtaataagt tcggttttaa cgaacatctg ttgcatgaca tgcaccatcg
      481 cctgaccccc atcgaagcgg cgcgctttgg gaagagcatt gaggattacc gcctgttctg
      541 gatggaagat ccgacgcctg ccgagaacca ggaatgtttt cgtctgattc gccagcatac
      601 cgtgacgccg attgcggtcg gtgaagtgtt taacagtatc tgggactgca agcagttaat
      661 cgaagagcag ctgattggct atattcgcac gacgatcacc catgcaggcg gcattaccgg
      721 gatgcggcgg attgccgatt ttgcatcgct ttatcaggta cgtaccggtt cgcatggccc
      781 ttcagattta tcgcctgttt gtcatgcggc ggcgctgcat tttgacctat gggtaccaaa
      841 ttttggcgtt caggagtaca tgggctattc cgaacaaatg cttgaagttt tcccgtacag
      901 ctggcgcttc gaggaaggtt atatgcaccc aggcgatgaa ccggggctgg gtatttcttt
      961 cgatgagaaa ctggcggcga aatatccgta tgacccggag tatctccccg tcgcacgact
     1021 ggaagatggc acattgttga actggtaata acggttcgct taattatttt ctgaatagtc
     1081 atcaaatcga ttaattccgc tcaaggagct aaaaatggac gttgatgtgt caaccagtgt
     1141 tgcgggtaat aaaccgcaac ggattcgtcg tattcagac
//
LOCUS       DAJTNP010000272         1166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTNP010000272 DAJTNP010000000
VERSION     DAJTNP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..102
                     /locus_tag="OO160_004280"
     CDS             <1..102
                     /locus_tag="OO160_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526126.1"
                     /translation="CELVLPAGTLRLKGKLTPALLQILIREIKGSSH"
     gene            102..449
                     /gene="tnpB"
                     /locus_tag="OO160_004281"
     CDS             102..449
                     /gene="tnpB"
                     /locus_tag="OO160_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526127.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            469..>1166
                     /locus_tag="OO160_004282"
     CDS             469..>1166
                     /locus_tag="OO160_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526128.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          269 a          323 c          331 g          243 t
ORIGIN      
        1 tgtgagctgg ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta
       61 ttacagatac ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag
      121 gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg
      181 tctcaaaagt tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc
      241 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
      301 ttaccagacg cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc
      361 accttactcc ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac
      421 gaacggaacg cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
      481 cttgctcatg agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
      541 cagatggcga aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc
      601 aaccggctga aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa
      661 aaacttcgcg caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt
      721 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg
      781 cgccagtctt cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc
      841 cgtccggaag aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat
      901 gtgtcagagc agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
      961 aaactggcct gttgccggtg tgaccatatc gtgcaggcac cggtgccttc aaaacccatt
     1021 gcacgcagtt atgccggagc ggggcttctg gcccatgttg ttgctggaaa atatgcagac
     1081 tatctgccgt tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt
     1141 gccacactgg ggcgctggac aggtgc
//
LOCUS       DAJTNP010000273         1140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTNP010000273 DAJTNP010000000
VERSION     DAJTNP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>1140
                     /locus_tag="OO160_004283"
     CDS             join(<1..233,233..>1140)
                     /locus_tag="OO160_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5526129.1"
                     /translation="GKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIKKY
                     GPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNSCC
                     WPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLD
                     LRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDER
                     WVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQ
                     VLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIY
                     GTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          303 a          272 c          306 g          259 t
ORIGIN      
        1 cggtaagcgt tatcccgaag agtttaaaac tgaagcagtc aaacaggttg ttgatcgcgg
       61 ttattctgtt gccagcgttg caacacgtct cgatatcacc acccacagcc tttatgcctg
      121 gataaagaag tacggtccgg attcttccac taataaagaa cagtcagatg ctcaggccga
      181 gatccgccgt ctccagaaag agctgaaacg ggttaccgac gaacgggaca tattaaaaaa
      241 agccgcggcg tacttcgcaa agctgtccga ctgaggtacg cctttatccg tgacaactcc
      301 tgttgctggc ctgttcgcct gctctgtcgg gtgctggatg ttcatcccag tggtttttac
      361 gcctggcttc agcagccgca ttcacaacgc catcaggcag acctgagact gacaggacag
      421 attaaacagt tctggctgga atcgggatgc gtctatggtt atcgcaaaat ccatctggat
      481 ctgcgtgaca gcgggcaaca gtgcggagta aacagagtct ggagactgat gaaacgtgtc
      541 ggaataaagg ctcaggtcgg ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc
      601 gtgtcaccca acaggctcca gcgacagttc aatccggatg ctcctgatga gcgttgggta
      661 acggacataa cctacatcag gacccacgaa ggctggctgt atcttgccgt tgttgttgat
      721 ctgttctcac gcaaaattat cggctggtcc atgcaatccc ggatgacaaa ggacattgtc
      781 ctgaacgcac tgctgatggc tgtatggcgg cgtaatcccg aaaaacaggt gctggttcat
      841 tcggatcagg gcagtcagta cacaagccat gagtggcagt cgttcctgaa atcacacggc
      901 ctggagggta gcatgagccg tcgcggtaac tgccatgata atgcggttgc agaaagtttt
      961 ttccagttgt tgaaacgtga acggataaag aaaaagatct acggaacgcg ggaagaagcc
     1021 cgcagtgata tttttgatta catcgaaatg ttttataaca gtaagcgtcg gcatggttct
     1081 agcgaacaga tgtcaccgac agaatatgaa aaccagtatt atcaacggct cggaagtgtc
//
LOCUS       DAJTNP010000274         1090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTNP010000274 DAJTNP010000000
VERSION     DAJTNP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..622)
                     /gene="ipaH4.5"
                     /locus_tag="OO160_004284"
     CDS             complement(<1..622)
                     /gene="ipaH4.5"
                     /locus_tag="OO160_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5526130.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKY"
BASE COUNT          309 a          199 c          237 g          345 t
ORIGIN      
        1 aatattttaa gctctgagga aattctggta gttcctgtag tctgttgccg gaaacaaaga
       61 gttcttgtag tctatgaggc aattcaggca agacagtaag gcgattacct tcaatacgta
      121 atattgttag cgttgatgga agctctggta acgtctccag actattaaaa cgtgccgaga
      181 gtgattgtaa atatggtggt aatgatggca gacatgacaa atttcgattg aatgaaatat
      241 taagcttaat cagttgagat ggaagtgtcg ggagtatatt caagttgtta ccatttacat
      301 gaagttctgt caaaagcgga gaattttcag gtagggagat taactcattg ttggaaatat
      361 taagttcacg tatatgcaaa gggagtggtg gtaaagaacg tagtttcagt aagcttaaat
      421 tgagaacagg ctcccgggta tccagacata ttttcaataa tcgaaccgcc tggatgcgat
      481 tttctgttgt tgttccttcc ctagcccagt catcccagat gcggtggtaa tctctggtac
      541 actgttcttc taccgataaa cgagatatac atgataagcc acaaagggaa cgaaaaaaag
      601 aatgattgtt gatcggtttc atttattaac ccctcactat tcatatcttg gtctattaat
      661 gcaaataaaa cgagaaagtc tttaaaaaaa caatcctaga gctttatcgg cgttttcatt
      721 ccggctggag gaatatgttc ctgtctttcc agtaaccgtc gattgttata tcagtctagc
      781 catgttaatg tgaccagttc tactttcatc cagtttggat tgatctttat cccctatatt
      841 ttcaaacaat ttttatcatt taactcatta ctgtctcgat attcccatag caaacgatct
      901 ggttggttat cccctcaaaa aagtcagttg cacgccgtaa atccctgccg gtattgagcg
      961 aggatttacg ggttgttgtt aaggtccgta atcgggtcac agtttttccg gagtcaatcc
     1021 ggtctgcggt ttatgcttat gcgacgtgat tatgaatggt gcagtcgtga gccgttttca
     1081 gacaatcgca
//
LOCUS       DAJTNP010000275         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTNP010000275 DAJTNP010000000
VERSION     DAJTNP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO160_004285"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO160_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5526131.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          347 a          158 c          194 g          389 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt
     1081 gcttgcgt
//
LOCUS       DAJTNP010000276         1086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTNP010000276 DAJTNP010000000
VERSION     DAJTNP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            25..570
                     /locus_tag="OO160_004286"
     CDS             25..570
                     /locus_tag="OO160_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5526132.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            594..>1086
                     /locus_tag="OO160_004287"
     CDS             594..>1086
                     /locus_tag="OO160_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5526133.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSE"
BASE COUNT          307 a          243 c          300 g          236 t
ORIGIN      
        1 gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactgc gggtaatccg
       61 aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa
      121 gacggaaaaa actggtatga agagctgaag aatttcgcca gcgacacaat aaaaatagct
      181 tacaccggag atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat cgagccacgc
      241 aatgccagcg ttatcgaagt tccggatatt accgctaacc gacggattac cgcgccgggt
      301 tactggtttt accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag
      361 cgtgatgccc ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg
      421 ctggggttaa tcagcgacga agataaagaa aagctgaaag cctgtcgtat ttacgcgaaa
      481 tcgctgcagg cgatggattt cagcaccatc actgataaat cctcatacaa cgccattgaa
      541 tggcccgcct ctccggaagg ttcttcctga tttaatttat cgcgagaaaa acaatgtctg
      601 tagtgatatc aggtgcgctg actgatggcg caggtatccc catgtccgga taccatatta
      661 ttctgaaatc ccgggtaaac accccggaag tggtgatgaa cactgttgct gatgtgatga
      721 caggaaacga tggtgaatac tgtttccatg cgcggactgg aaaatatggt gtgtatctga
      781 aacaggactg gcgcaacgag tacaacgttg gcgacattgc tgtatatgag gactcaaagc
      841 ccggcacgct gaatgacttt ctgattgctc ctgatgaggg cgacctgaaa ccggatgtcg
      901 tcaaacgctt tgaggaaatg gtggcgcagg cgcagcagag cgccggggcc gcagccggaa
      961 acgcacagca gacggcgcag gatgtggcgg cagccgcagg ttatgcccgc gcagcagagc
     1021 aggccaaaaa tgacattgat gctgcgctga ccggcactct gaaaacggct aaccatctgt
     1081 ctgaaa
//
LOCUS       DAJTNP010000277         1048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTNP010000277 DAJTNP010000000
VERSION     DAJTNP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..132
                     /locus_tag="OO160_004288"
     CDS             <1..132
                     /locus_tag="OO160_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5526134.1"
                     /translation="NYEVMKTLLGERVMDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            147..569
                     /locus_tag="OO160_004289"
     CDS             147..569
                     /locus_tag="OO160_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5526135.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            627..983
                     /locus_tag="OO160_004290"
     CDS             627..983
                     /locus_tag="OO160_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526136.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          301 a          207 c          292 g          248 t
ORIGIN      
        1 aactatgagg tgatgaaaac attgctcggc gagcgggtga tggatcgcat ggtcatgaac
       61 ggcgggcgct gggttaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
      121 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
      181 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
      241 tgtccgatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
      301 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
      361 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
      421 atccggacct tatctggtca ttacctgacg gagaaatacg ccgctacgac aggcgcctga
      481 atataatctg tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
      541 agggtaattt tcaggaggcg atactgtgaa tgaaattagc tatcaggctt caattaccgc
      601 tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag ttaagcaata
      661 tcgttttatt tccgataaaa gaggatgacc ctcgtaatca ggttaatttt ctttataagc
      721 catcggaaag accatattgc catcacgcct ctgtacgggt tgacgaaaaa gagcgtcagg
      781 tccgctgtaa aatctgcggt gcggttgtgg agccgtttga ctggatgctc tctgtggcaa
      841 aaagagaaac cagactggca gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc
      901 ggagaaatat agaaaagctg atacagattg agcgtaacgc gaaagcgcgg atacgcaggg
      961 cgacaaaatc cagaactgaa taattaaatt tagcactgtt aaaaatttaa tccttaaccg
     1021 gagggatttc tgcaccctca aatcatca
//
LOCUS       DAJTNP010000278         1022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTNP010000278 DAJTNP010000000
VERSION     DAJTNP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(120..809)
                     /locus_tag="OO160_004291"
     CDS             complement(120..809)
                     /locus_tag="OO160_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5526137.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(806..>1022)
                     /locus_tag="OO160_004292"
     CDS             complement(806..>1022)
                     /locus_tag="OO160_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5526138.1"
                     /translation="SLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQ
                     FDETNIVRGLPVPGGRLGIKITELECA"
BASE COUNT          242 a          282 c          203 g          295 t
ORIGIN      
        1 cgtttaacgt cctgtgccat ttttcaggca taaaaaaacc gctcggcggc gggttttgct
       61 tactttacca tcgcgtacaa aatcggcaaa gtatcagatt tgcatgagat atatgccttt
      121 taatctactt ttgcaatact ttgctttgaa aatgccgctt tttgttctga acgtgttctc
      181 atcacaaaca ataaagcctc actatccagt cggtgaaaaa tgtgtttcat tacaacccag
      241 tgaccagtaa atgttttgga ccagtttttg gttgttactc ccgccagtaa tgccagctcc
      301 tgatattcat aaccttcccc accaaaaagt tctgctttta ccgcctgcgc cgccagccag
      361 attaattttt tcaggcgttc ctgtgttttc tctgcaattt tcctagtgcc agaatgagcc
      421 ttaaactctt cccacgccca gtgggttatg gtgatctgat actcccagca aatacttccg
      481 ctgtaacacc acaacaacca ggctttatag tgctcttcaa gagacagaac agcccgccgc
      541 cacgatgatg tcgaaaacgc aacctgactg accagaggaa ttgacgcccc cttcgccagt
      601 gattgttttc ccgggattgg tggattatcc cgcgttatca tttttccggt cacctcatcg
      661 cggtaccgga tttttttacg cctgtaacgc cctgtatcga acatggcatt ctcctgccag
      721 gcttcaagct gaccttttgt cgccccactc aaatcagcgg tggcgataat gagctgctca
      781 cgaacaaact gtaaatactg gttattcatg cacactccag ctctgtgatt tttatcccca
      841 accgaccacc aggaacaggc agtccgcgca caatattggt ttcatcaaac tgctcgtcgt
      901 cgataagcaa ccccgcatgt gtcagtgcat ccagtggtgc tttcaggata ttgtccaggt
      961 cacgacgccg cttatccggt ggctctgcaa taatctttat cgccagtctt ccggacaggc
     1021 tt
//
LOCUS       DAJTNP010000279          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTNP010000279 DAJTNP010000000
VERSION     DAJTNP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO160_004293"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO160_004293"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5526139.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTNP010000280          957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTNP010000280 DAJTNP010000000
VERSION     DAJTNP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(3..485)
                     /locus_tag="OO160_004294"
     CDS             complement(3..485)
                     /locus_tag="OO160_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5526140.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO160_004295"
     CDS             complement(476..778)
                     /locus_tag="OO160_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5526141.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          289 a          235 c          193 g          240 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgat
//
LOCUS       DAJTNP010000281          954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTNP010000281 DAJTNP010000000
VERSION     DAJTNP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..216
                     /locus_tag="OO160_004296"
                     /pseudo
     CDS             1..216
                     /locus_tag="OO160_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            362..>954
                     /locus_tag="OO160_004297"
     CDS             362..>954
                     /locus_tag="OO160_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5526142.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRG"
BASE COUNT          234 a          232 c          292 g          196 t
ORIGIN      
        1 gcacaccgag cagggaattc tggttatcgg tggggctgtt cgccagaaaa aatttgaccc
       61 gttgtcagtt gatctgcccg actggttgtc agaaacactc tggcatgagt gggtccagtt
      121 caggcaggca ttgcgaaaac cgattcgaac ggagctgggc gctaacgggg cgatacggga
      181 actggaaaaa ttccgtcagc agggttttac acctgagcag gtgattcgac acagcatcgc
      241 caatgaatac cagggtctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca
      301 ggttaacacc gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacagac
      361 catgaaaaat attgcgacag gaggcgttct ggagcgtatc cgcagactga ccccaccaca
      421 tgtaaccgcc ccattcagaa cggttgcgga gtggcgcgag tggcaacttg ctgaaggcca
      481 gaaacgttgc gaggagatca accgcctgaa tcgtcagttg cgggtggaaa aaatcctgaa
      541 ccgctccggc atccagccgt tgcaccggaa gtgttcgttt gcgaattacc gggcgcagaa
      601 cgacggtcag cgacatgcac tgagtcaggc gaaatccata gctgacgaac tgatgacagg
      661 ctgtacgaat tttgtgttca gcggtaagcc cggcaccgga aaaaatcatc ttgcagccgc
      721 gattggcaac cggctgatgg cgaaggggcg tagcgtgatt atcgtcaccg tgtccgatgt
      781 catgagcgtg ttgcatgaga gctacgacaa cggcaaatcc ggtgaaaaat ttttacagga
      841 gctttgtggt gttgacctgc tggtcctgga tgaaattggc atgcagcggg atacgaaaaa
      901 cgagcaggtg gtactgaacc agattgttga tcgccggacg gcatcgttac gcgg
//
LOCUS       DAJTNP010000282          951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTNP010000282 DAJTNP010000000
VERSION     DAJTNP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            24..951
                     /locus_tag="OO160_004298"
                     /pseudo
     CDS             24..951
                     /locus_tag="OO160_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          263 a          236 c          262 g          190 t
ORIGIN      
        1 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
       61 aatccgctca actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg
      121 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      181 aaacaccaaa agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      241 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      301 cagactccct gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac
      361 actctgccat gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga
      421 aaaaccagac ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag
      481 ccacggttcg gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat
      541 gggacgctgg cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc
      601 gactcaccgg tataaacgtg gtggtcatga acatgttgct atccctaact accttgaaag
      661 gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt gatgtgacct atatctggac
      721 gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg
      781 ctgggccatg tcgttctcgc cggacagcag gctcaccatg aaagcgctgg aaatggcatg
      841 ggaaacccgt ggtaagcccg gcggggtgat gttccacagc gatcagggca gtcattatac
      901 gagcaggcag ttccggcagt tattgtggcg ataccagatc agacagagta t
//
LOCUS       DAJTNP010000283          937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTNP010000283 DAJTNP010000000
VERSION     DAJTNP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(191..856)
                     /locus_tag="OO160_004299"
     CDS             complement(191..856)
                     /locus_tag="OO160_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5526143.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          242 a          231 c          211 g          253 t
ORIGIN      
        1 aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc ttgccggacg
       61 cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct gaagcagata
      121 gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat cagaaaggtg
      181 ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt ttgataagag
      241 ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca cagccacaat
      301 attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac gagccgccct
      361 gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg gctcgccgcc
      421 cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa ctgctgatca
      481 cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca
      541 tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt tcaaaccagt
      601 tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca aagcgaatat
      661 tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg tagaaaaaga
      721 tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa catggagcac
      781 agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg ggccgtttga
      841 aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag agtgtagcat
      901 tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTNP010000284          936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTNP010000284 DAJTNP010000000
VERSION     DAJTNP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            144..641
                     /locus_tag="OO160_004300"
                     /pseudo
     CDS             144..641
                     /locus_tag="OO160_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="OO160_004301"
                     /pseudo
     CDS             682..873
                     /locus_tag="OO160_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAJTNP010000285          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTNP010000285 DAJTNP010000000
VERSION     DAJTNP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..92
                     /locus_tag="OO160_004302"
     CDS             <1..92
                     /locus_tag="OO160_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5526144.1"
                     /translation="LAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            104..475
                     /locus_tag="OO160_004303"
     CDS             104..475
                     /locus_tag="OO160_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526145.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            462..>932
                     /locus_tag="OO160_004304"
     CDS             462..>932
                     /locus_tag="OO160_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526146.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          210 a          208 c          290 g          224 t
ORIGIN      
        1 ctctggcatt tgtgccgccg ggaatggcgt tccgtgtctt tgccggtgtg gcagccggaa
       61 tgacagaacg tggcctggcc agaatgcaat aacgggaggc agcgtggctg atttcgataa
      121 tctgtttgat gttgcacttg atctcgcaga caaggccatt attcgcaata tggggattag
      181 agcggtcatt acgtcaggcc ggctaaaagg gatcatgatt tccggggttt ttgatgatcc
      241 tgaaaatatt tctctggtgg ccggcggtgt gcgtattgaa gactctttac catccctgtt
      301 tgtgaaaaca gcagatattt tacggctgtg tcgcaatgat tcgctgatga ttggtcgtga
      361 gtctttctgt gtggatcgta tcacccctga tgatggcgga tgtagttata tccggttgcg
      421 gcgtgagggg ctgccgggaa acgtaagggc aggacgatat tatgaagggg cttgagaatg
      481 ccatccgcaa tctgaacagc cttgataccc gtatggtgcc acaggccagc gcatgggcga
      541 taaaccgtgt ggcacagaaa gcggtctcgg ttgccacccg gcaggttgcc gggaataccg
      601 ttgcgggcga taaccaggtg aaagggatcc ccctgaaact ggtacgtcag cgtgtccggg
      661 tgtttaaagc cagtccgtca ggaaaaatga cggccaggat ccgcgttaac cggggcaatc
      721 tgcccgccat caaactgaac acaacacggc ggcgtgctgg tgaaggactg agagtgggaa
      781 aatacttttt ccggggggca tttgttcagc aactggcgaa tggccgctgg catgttctga
      841 ggtgtcttcc tgaagcgcgt tttgcaacag ggcatgacca tcagggcagg ctaagaaaaa
      901 atcgtcttcc tgtggaggta gtgaaaatcc cg
//
LOCUS       DAJTNP010000286          891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTNP010000286 DAJTNP010000000
VERSION     DAJTNP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            274..747
                     /locus_tag="OO160_004305"
     CDS             274..747
                     /locus_tag="OO160_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5526147.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          270 a          188 c          198 g          235 t
ORIGIN      
        1 tcgcaatcgc tggattcggt cattctgtct cccttgtata tgggttaacc agataaccac
       61 ggggagcagg taccggcatc cacgttacag acggtcccgg caaaagtgcc tgacctgacc
      121 cctattagcg gttaccagcg ccccaccaga cccggtgaca tcaccccccg gatcatggac
      181 gactgggggc agtaatcatg ccgggtctgg ctggctaaca ccccattata aggggtacga
      241 ataaagtaac gggggggaga tgaaccctat gcaatgtcct ggttaatatc tcagtgtgct
      301 catcagtgca ctgataataa aaagacggag acagatgcca tttacgataa agttcggagt
      361 tcttatttgc tttcatgtat tctgaaaaaa aacaaaaatg ttgggcttat tcttcatgca
      421 ccatcatttg tttctgtgtc tgaaaaaatc gccagaattg tgatggcaaa ttactcccgt
      481 aactggagta attccgagtt ggccagtgcg gttttaatga gtgaatcttc tttaaaaaga
      541 agaatgtaca aagaggtcgg ttctataagt acatttgtac ataaaataaa gcttacagaa
      601 gctatcagga aattacgaag gacaaatact cccatttctg tcatatcatc tgagttaggt
      661 tacagttcac catcgtattt ttctaaagtc ttctttaaat atttaaagac ataccctcaa
      721 aacataagga agaaaaatgg tcggtaatgt caccaattag gaggacttgc agagccgctt
      781 ttttacgata ctgagatcaa acagacataa tctatgcgga gatttacagc agggcatggt
      841 tcacaaatct gacagcgatg aattatcggc actgcgggca gaaaatgctc g
//
LOCUS       DAJTNP010000287          889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTNP010000287 DAJTNP010000000
VERSION     DAJTNP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            218..757
                     /locus_tag="OO160_004306"
     CDS             218..757
                     /locus_tag="OO160_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5526148.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
BASE COUNT          373 a          177 c          144 g          195 t
ORIGIN      
        1 aaaaaacacc gcaaaaatgg attgttaaca aatgaaaata ctcaattcga atctcataac
       61 aaacatatca tcaacaataa gaaatcacct tctaacatca tccaaaaata taaaaacaca
      121 cccatcacca aacatggacg taacaagtca tcgtcgttta ttccagaaag acggaatagg
      181 attgagccaa acaggaaaag caacacaact tgtcattatg tcgcatggtg gatggaaaga
      241 gatctcccat ccacaaacac tattcacgcg tcaaaaaggc gatggatgga ctgtagtccc
      301 caaaaaccta agaattgatt tttatacaaa agacaacgac ttcacaaagg gactaagcgt
      361 tctttcagag gtaaacaaac gccatactga agctcaaaaa gggttaacac catcactaaa
      421 cataagcaaa gatgacctaa aagttctagc gaacgcgcgc aatatacctc aatcaaaatt
      481 agaagaagaa atgatgtcac aagccatata tagaaaagaa tacgcaacaa gtaatgagaa
      541 gataaaaaac tatgctctat attaccatga acaagcccaa aatattatac aaagacatca
      601 agatggctta ggaaacaaaa atattgatat agcatttata actgataaaa atcataagaa
      661 gcacctttct gacatattta aagtaataaa tgaatcagga gtaaaatatg atgtaattca
      721 ctttggcgct tgccgagtaa atagagagga gaaataatca tcatttacca taatccctta
      781 attgtacgca ccactgaaac gcgctgaacg cgattctgac gcagacacca caaaaataga
      841 tgttttaccc cggaaaaaca ggaaggatgc gtcaggatcg ttttcagct
//
LOCUS       DAJTNP010000288          819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTNP010000288 DAJTNP010000000
VERSION     DAJTNP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(142..478)
                     /locus_tag="OO160_004307"
                     /pseudo
     CDS             complement(142..478)
                     /locus_tag="OO160_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          263 a          155 c          148 g          253 t
ORIGIN      
        1 agaacttctt cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa
       61 ctgaactaat acatcagaat ctgcattatg ttaaataaat atgaaaagat ggtttaaata
      121 ccccgttact tttgacttac actatacggt atcgcatcgt ttaatattcg caccggccag
      181 atttttattt ctattagttg tcacaatact gaatgcgtac gaccacagta ttctggctcc
      241 tgtgtggtta tgctttaatt ctgcgttccg ggcagataag cagttgcttg caggaatcct
      301 tcttgtgtta atgtcagttc ccctttttac cagtgctgat ttccacattc cgtccaacag
      361 agcttatagc ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa
      421 tagaagcact gctggcagat ccagtccgtt tttcataacc cactgtactg ataaccataa
      481 tctaatcagt agaaattgag tcgaaaataa gcactactct atacaggata attagaggtc
      541 agtttgatta ttcacaattc atcatcagca ttttctattt ctgacgaaat caacatgaaa
      601 ataaccatat atgataatta ttataataac ggctttaatt ggaatacata tattacaacg
      661 tattatatat aattggtatt ctgggaacta tattctcaaa atacagtaga aacgaggtat
      721 gtttctggtg gaaaggacag tgggattaaa aagtaagaat tgataaaaaa aacgccagca
      781 acacatagtg ccggcggagg gaatacccca tggagaaaa
//
LOCUS       DAJTNP010000289          819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTNP010000289 DAJTNP010000000
VERSION     DAJTNP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>819
                     /locus_tag="OO160_004308"
     CDS             <1..>819
                     /locus_tag="OO160_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5526149.1"
                     /translation="YQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKS
                     TRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLA
                     EKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLT
                     VDADPQAEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQ
                     QKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNN"
BASE COUNT          245 a          187 c          200 g          187 t
ORIGIN      
        1 tatcagtcgc tgtttttccg tgtaaatgca cgagacgata atatgcagga tgctgcgaat
       61 gcattaatgg cagagttggc aaccattgat cagcatggtt tttctgctga agaactcgat
      121 gatgtcaaat ctacccgcct cacctggctg aaaaatgcgg ttgatcagca agctgaacgt
      181 gatttacgta tgctgaccag tcgcctggca tccagctcat taaataatac gccgttcttg
      241 tcgccggaag agacatatca actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt
      301 ctggcggaaa aatggcagca gttaagaaag aaccaggacg cattttggga gcaaatggta
      361 aacaatgagc ttgccgccaa aaaagcattg tctcctgcgg ctatcctggc gctggaaaaa
      421 gagtacgcca acaaaaagct ggcggcttac atcttcccag gcagaaattt atcgttaaca
      481 gtagacgctg acccacaggc ggaaattagc agcaaagaaa cgctggctga gaatctgaca
      541 tcattaacac tttccaatgg tgccaaggtt attctggcaa aatccgcggg tgaagagcaa
      601 aagctacaaa ttattgccgt atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa
      661 tcacttatcg cgctggcaaa taaagcagtt agcggaagcg gcgttggcga actctcctct
      721 tccagcctga aacgctggag tgcggaaaac tcggtaacca tgagcagtaa agtcagtggc
      781 atgaatacgt tgctctccgt tagcgcgcgg actaataac
//
LOCUS       DAJTNP010000290          818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTNP010000290 DAJTNP010000000
VERSION     DAJTNP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(21..311)
                     /locus_tag="OO160_004309"
     CDS             complement(21..311)
                     /locus_tag="OO160_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5526150.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(311..>818)
                     /locus_tag="OO160_004310"
     CDS             complement(311..>818)
                     /locus_tag="OO160_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5526151.1"
                     /translation="GEWIHADFKRVRNYAFHKRFFKLLQLGFDYWTPVGGAITPRERK
                     LVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDTALLKSFDAFREWVTIQAG
                     FYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVLWNWILFRKFSSPEEVENV
                     AAQLLEFA"
BASE COUNT          194 a          221 c          186 g          217 t
ORIGIN      
        1 gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc ttgtgtgcga
       61 aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct ggtttttacc
      121 cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctacctgcat atcgtgtggt
      181 tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga agtttcggga
      241 ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc cgctttacgt
      301 aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact tcctccggag
      361 aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat ttataaacct
      421 gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg cgcccacgac
      481 taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc cactcgcgga
      541 aagcgtcaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca actgtattaa
      601 ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact gattcgcaca
      661 ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc ccaccgaccg
      721 gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg aatgcgtagt
      781 tacgcacacg cttaaagtct gcgtgtatcc actcacct
//
LOCUS       DAJTNP010000291          810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTNP010000291 DAJTNP010000000
VERSION     DAJTNP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..209)
                     /locus_tag="OO160_004311"
     CDS             complement(<1..209)
                     /locus_tag="OO160_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5526152.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTR"
     gene            complement(364..522)
                     /locus_tag="OO160_004312"
     CDS             complement(364..522)
                     /locus_tag="OO160_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5526153.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(593..>810)
                     /locus_tag="OO160_004313"
     CDS             complement(593..>810)
                     /locus_tag="OO160_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCR5526154.1"
                     /translation="SRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLG
                     VQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          189 a          205 c          226 g          190 t
ORIGIN      
        1 cgggtctggc aggcgaagaa tggctgaaaa ccttcctgct gcaggctttc cagtacggtg
       61 atggtgggaa tgtacgcata tcgtttactg cgggaagtat gccggtcttc accgaaaata
      121 ctgggaacat agtgcatcag ttcttcgtgt gtcagcggac ggtcacggcg tatctggttc
      181 gcataaccaa aacgactggc tagtcgcata atttgctcct tatcggtggt tacgatttac
      241 tggtgtaata aatgaaaaag ctgcgtctcc cggagaagac gcggcctgac agatgaaatg
      301 aatgacgttt attgtctgag aagcccttaa ctggcgagct gagtattaag ctgtgttccg
      361 gcatcaccag cgcaactgac cttcagcatt acggataacc agccgggaat atgttccctg
      421 gtcatcttca gtaaacacat tgcggtaagc tgttttgacg gcaacagcct gttcgcgtga
      481 gaaagggcct tcgggcaata aacgtgatgt acaacccggc atatctgttg ctccctgaaa
      541 gtaaaaagcc ccggtcatga tgaccggggc ctgaaggaga gtgacctgat tatcaaaaag
      601 tcacattcag cgtggcctga ccgttataac cttcagcgct gctgccgctg acgctgtggg
      661 cataaccggc ctgaacgccc agggtgatat tttcccggac acgggcttcc agtccggcct
      721 gcaggtccag tgacgtgcca ttccgggacg gtgagaacgt catgttactg ccggcggcgg
      781 ctgtccccat gctcatgtca ccccgggagc
//
LOCUS       DAJTNP010000292          758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTNP010000292 DAJTNP010000000
VERSION     DAJTNP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO160_004314"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO160_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5526155.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          277 a           98 c          149 g          234 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa aaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtgg
//
LOCUS       DAJTNP010000293          756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTNP010000293 DAJTNP010000000
VERSION     DAJTNP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..390
                     /locus_tag="OO160_004315"
     CDS             <1..390
                     /locus_tag="OO160_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5526156.1"
                     /translation="EDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLF
                     RKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGI
                     LIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT          204 a          204 c          187 g          161 t
ORIGIN      
        1 gaagaccaga agaagcgagc agccattcag tccgctaacc gccgaggcgt gacttggggc
       61 gactactgca aaacctatgg tctgcccgct cagaagctaa tgaccgcact tctccagcat
      121 cgtggcctct ttcgtaagaa cccaatcagt aacgagtggt ctgtaaatcc gaagtattcc
      181 gactgctttc gtatcattaa gccgtcagac cagaagttct cagcaggtgg gtataacttc
      241 cggttcaacg ctaaggggct ggaggtcttc ggtaagcctg agatggttga caagatgcga
      301 ggcatcctta ttgcattcac tggcaccgac cagcagaagc aagagcacct cctgaagctg
      361 gcacaatccg gtaaggtgga gggaatctaa gatgatgatt gacccgcgca cacctgaggg
      421 gcgcatgact ctccgttacc gtggctaccg gactgaagtc ctcctcagag agcttggtct
      481 agacccggaa gatgagaccc gccagcacca atcccgagac gagctaatag cccaactggt
      541 cgctatgaaa ctgccgctta accgttagta gctcacctac tcaataccta atttaaacac
      601 cttttgagat gaccgatgcg gggagacccg taggccttac tgtgcctgcg ataccgatag
      661 tctcaactct caagaggaga attacctatg atgttcaaca acaacaactg gaagctgtca
      721 gtcaccgaca tcaaccttta tgaaaacact gtgagt
//
LOCUS       DAJTNP010000294          748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTNP010000294 DAJTNP010000000
VERSION     DAJTNP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..214)
                     /locus_tag="OO160_004316"
     CDS             complement(<1..214)
                     /locus_tag="OO160_004316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526157.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQ"
     gene            complement(347..652)
                     /locus_tag="OO160_004317"
                     /pseudo
     CDS             complement(347..652)
                     /locus_tag="OO160_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          173 a          190 c          181 g          204 t
ORIGIN      
        1 actgtcgctg aagtctcgcc agcgtcttct ggtttttctg aaaactgttt acaggctcaa
       61 agactgtgcc atctgacagc gaggcaagtt tagccacgcc agcatccagc ccgaccattg
      121 atgctgaagg gtgaaccgga gttgatactt cactttctgc ctgaatactg atgtaccact
      181 taccgcagga ctggctgaca gtgacatttt tcacaacacc cgtgacctga cggctgttcc
      241 ggtagcgcat ccagccaagt tttggcagaa aaatacggct gttttcctga gcgagcttaa
      301 cgccctgcgg atagcggaat gcatcattct gtcaccgctt tttgaatcag gggaacgccg
      361 agcgcttctg gaaaaagttt ttgcaggcct gctcagggtc tttcagtgac tgttgcaatg
      421 gctgtgacgg agcatcttta atccattgcg tttcagtggc atttttccac tcaaccaatc
      481 aggaagccat tttcgcgtaa gggatgtctt tgttaccggc cttatgattc tcattctgac
      541 gtgccagagt acgactgaaa acgaaacgac atgcgcctgc gaagcgcctc atttcacgcg
      601 cctgttgacc accgggtctt aaccggaatt tgaatacctg tagtcgtttc acagggcaaa
      661 ttatactttg gtctgtgaaa aaaactgata tccgacgcgg aaggcactgt gttttcctga
      721 tgcatgctca ccgggtcttt gttaccaa
//
LOCUS       DAJTNP010000295          730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTNP010000295 DAJTNP010000000
VERSION     DAJTNP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            133..562
                     /locus_tag="OO160_004318"
                     /pseudo
     CDS             133..562
                     /locus_tag="OO160_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          180 a          146 c          233 g          171 t
ORIGIN      
        1 tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtgt ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      541 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      601 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      661 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      721 aatcttttgg
//
LOCUS       DAJTNP010000296          728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTNP010000296 DAJTNP010000000
VERSION     DAJTNP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..114
                     /locus_tag="OO160_004319"
     CDS             <1..114
                     /locus_tag="OO160_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5526158.1"
                     /translation="IGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            342..>728
                     /locus_tag="OO160_004320"
     CDS             342..>728
                     /locus_tag="OO160_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526159.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          227 a          110 c          151 g          240 t
ORIGIN      
        1 attggtacag agctgggaat ggaccaggat gaagtgctga ggctgaaaca gattagcggg
       61 ctgacagagt tgtttcagga agaggatttc agctcggctt ggacggttcg ttgatgttgt
      121 taaatatggg cggtatttct gaaagttgat actttgggta gaaaaaatga taccgaaaac
      181 tatggaggat gtgcttgtca ggtgactgga caccataggt cttaggcttt gataacggga
      241 taagatgagc ggcttttctc ataccatcag gtgtgatgat ggcgcagatt tacaaaatct
      301 gattgattct aaagatgtat aagtaaaaat caagctttga tatgaaaaaa cagatattta
      361 ttaataataa acctcctgta gtcccttact cagggacgca cgcgaaaata tttaaataca
      421 ttgaaatccc tctgccgttt ttctacttta tctatacatc aggcgaacca ttccacatat
      481 ctgtccaaaa tactgttatt tatgtatcaa aatacaatgg tatatttatt aataaattag
      541 ttcctttttc tcttttattt gacagggata tatctgttct tcaaagaaga gatatttgtg
      601 ttgtgcgatt tacgtcagag gaaataagtg aacataacgt tttatttgat catgacattg
      661 aacgattaaa aaaaatttcc aaagctcagc ttatatctcc cgattatgtt ttgattgatt
      721 tttctagt
//
LOCUS       DAJTNP010000297          727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTNP010000297 DAJTNP010000000
VERSION     DAJTNP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>727
                     /locus_tag="OO160_004321"
     CDS             <1..>727
                     /locus_tag="OO160_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5526160.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWI
                     QLQRKTLSKHAEMAKAFDYILNHW"
BASE COUNT          195 a          170 c          223 g          139 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaatccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg
      541 atagcagagt tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt
      601 gcagtcagaa aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg
      661 cagaggaaaa cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat
      721 cactgga
//
LOCUS       DAJTNP010000298          720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTNP010000298 DAJTNP010000000
VERSION     DAJTNP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>720
                     /locus_tag="OO160_004322"
     CDS             <1..>720
                     /locus_tag="OO160_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5526161.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRP"
BASE COUNT          200 a          144 c          177 g          199 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
//
LOCUS       DAJTNP010000299          706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTNP010000299 DAJTNP010000000
VERSION     DAJTNP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            167..406
                     /gene="relB"
                     /locus_tag="OO160_004323"
     CDS             167..406
                     /gene="relB"
                     /locus_tag="OO160_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5526162.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            406..693
                     /gene="relE"
                     /locus_tag="OO160_004324"
     CDS             406..693
                     /gene="relE"
                     /locus_tag="OO160_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5526163.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          191 a          147 c          183 g          185 t
ORIGIN      
        1 gctgacctgc acaatacagg atggacttag caatggctgc tcctggcaca aagcggacag
       61 tgatcaccgt tcttacgact actttctgac ttccttcgtg acttgcccta agcatgttgt
      121 agtgcgatac ttgtaatgac atttgtaatt acaagaggtg taagacatgg gtagcattaa
      181 cctgcgtatt gacgatgaac ttaaagcgcg ttcttacgcc gcgcttgaaa aaatgggtgt
      241 aactccttct gaagcgcttc gtctcatgct cgagtatatc gctgacaatg aacgcttgcc
      301 gttcaaacag acactcctga gtgatgaaga tgctgaactt gtggagatag tgaaagaacg
      361 gcttcgtaat cctaagccag tacgtgtgac gctggatgaa ctctgatggc gtattttctg
      421 gattttgacg agcgggcact aaaggaatgg cgaaagctgg gctcgacggt acgtgaacag
      481 ttgaaaaaga agctggttga agtacttgag tcaccccgga ttgaagcaaa caagctccgt
      541 ggtatgcctg attgttacaa gattaagctc cggtcttcag gctatcgcct tgtataccag
      601 gttatagacg agaaagttgt cgttttcgtg atttctgttg ggaaaagaga acgctcggaa
      661 gtatatagcg aggcggtcaa acgcattctc tgaaccaaag catgac
//
LOCUS       DAJTNP010000300          670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTNP010000300 DAJTNP010000000
VERSION     DAJTNP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(1..589)
                     /locus_tag="OO160_004325"
                     /pseudo
     CDS             complement(1..589)
                     /locus_tag="OO160_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
BASE COUNT          189 a          141 c          131 g          209 t
ORIGIN      
        1 catcaacgat gacatccatc acactgatat gaattgggtt gggattctct gctatcagat
       61 ttcgcccttc ccggcgccat ttcactttcg gcgcagcatc ttcgggaata aggcggatat
      121 ttgctggtcg ccagaacagc ttgatacgcg tttttactgc tatttccagc gagttcgtcg
      181 ctttgttgct cgctgatggc ggaatatttt taatattcag ccagaagact gactcgcgat
      241 catcaggcaa tgacacacct ttggtgtgaa taatgcgtaa tattttttct tgcgaaggtt
      301 ctaatcgtga tactggcgga ataacggtaa acggcgcttt gcttttatcc tctttattaa
      361 atggatctac ccaagcctgg atgagataag gtattttttc ttctttgcta aatacagaaa
      421 tagatttatc agcattatta cttaagtaaa ccacccgcgt actaccaatg acaacgcctg
      481 cataagttaa tgctggttgg cctaaaaaaa cgctgagtat aatcgcggta aataacttac
      541 attaaactgt tgttttaaag agcctgatta aacaactttt tctggacata acgactccct
      601 ggccaaagcc aactgaataa aaaggccctt gataaattat ttcttatatt cgacagtaaa
      661 ttgtgaatca
//
LOCUS       DAJTNP010000301          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTNP010000301 DAJTNP010000000
VERSION     DAJTNP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>668
                     /locus_tag="OO160_004326"
     CDS             <1..>668
                     /locus_tag="OO160_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5526164.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTNP010000302          665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTNP010000302 DAJTNP010000000
VERSION     DAJTNP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..284
                     /locus_tag="OO160_004327"
     CDS             <1..284
                     /locus_tag="OO160_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5526165.1"
                     /translation="AFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVVHFDKFG
                     NVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLLSLK"
     gene            301..582
                     /locus_tag="OO160_004328"
                     /pseudo
     CDS             301..582
                     /locus_tag="OO160_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          213 a          149 c          140 g          163 t
ORIGIN      
        1 ccgcattcaa ggaagcctac attgctgagt tcgaccgcat ggaggccgaa ctgcgccaga
       61 ataacacccc acctgcagac aagatgattc cgggtgatgg tcgcactctg gttgttcact
      121 tcgacaaatt cggcaatgtc gaattcaccg aaaccgttcc tgatggcgca ctggtctgta
      181 ccctggagac tttccggttt tatctggaaa aacagggatg gactcttgtt aaccggggcg
      241 caattaaaaa tatgactgtg gaacaattac tttctttaaa ataaaaatgg agctacggat
      301 atgaacccta tgcttgatta ttgttttacc tctgcacctc agcagaccat aacacacacc
      361 ggaatcaaaa atttgttatg gataatcaaa aaaaacctcc agtagaaagg tgatcccgat
      421 ttaattccct gtgcttatgg cctgtgtgac agacaagtaa gccctgataa ggcttacact
      481 gctgaactta tttacatgca cggaaccatg atgaggaaaa agaaacgcaa atattgtagt
      541 gaaatctgcg ccgaaaaaga ccagatggca cacgaacttt aattaactga ctattcgaaa
      601 ctgaatttat gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga
      661 acacc
//
LOCUS       DAJTNP010000303          664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTNP010000303 DAJTNP010000000
VERSION     DAJTNP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..664
                     /locus_tag="OO160_004329"
                     /pseudo
     CDS             1..664
                     /locus_tag="OO160_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          143 a          167 c          210 g          144 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca gtgaaacagt
      541 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc
      601 agtggagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac gccagttcgc
      661 ccgg
//
LOCUS       DAJTNP010000304          661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTNP010000304 DAJTNP010000000
VERSION     DAJTNP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>661)
                     /locus_tag="OO160_004330"
     CDS             complement(<1..>661)
                     /locus_tag="OO160_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5526166.1"
                     /translation="PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAG
                     VRVVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDV
                     DDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEEL
                     YSAREFYQDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEP
                     IL"
BASE COUNT          160 a          194 c          145 g          162 t
ORIGIN      
        1 aaagaatagg ctcattcagt gggcatgcct ttccgtgacc gtgcacacca ttgaccagcg
       61 tcaactctgc ctcacggaac tggaatttat ctgccagaga ttcccacggt ggattaaaca
      121 gatactgctg cttgccgtag aaagcgttga tagtgtcctg gtaaaactct cgcgcactgt
      181 agagttcttc aggatcgaag taggatgccg tgccgatgta ctgccagatt tcatcctcgg
      241 taacaccgtt catcaggcat tcgttgatgt ctttgtacgg cagagtaaca agacggcaac
      301 gatgttcacc gagtcggctt acgatttccc ttgcggcttc acgaccaaca tcatcaacgt
      361 ccatcgagat gaatatttcc tcaaacctgt cgaggttgtg atactcaaac tcaatccact
      421 gttgcttagc gcctttcccg ccaccaaacg gcacggataa cgccgagatg ccgtattgcg
      481 catagctcat acaatcaatt tcgccttcgc aaagcacaac caccctcacg ccagcgtcca
      541 gagcctgcca tccgaacagg caaggttcgc aatcaccttc tgccataatg actttcttcc
      601 cgtccgggcg ctcagtgctg attcgcttga cctgcaacaa ctcaccatcg cgtttgtacg
      661 g
//
LOCUS       DAJTNP010000305          660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTNP010000305 DAJTNP010000000
VERSION     DAJTNP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>660
                     /locus_tag="OO160_004331"
     CDS             <1..>660
                     /locus_tag="OO160_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5526167.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     T"
BASE COUNT          153 a          167 c          208 g          132 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
//
LOCUS       DAJTNP010000306          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTNP010000306 DAJTNP010000000
VERSION     DAJTNP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            103..>659
                     /locus_tag="OO160_004332"
     CDS             103..>659
                     /locus_tag="OO160_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5526168.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTNP010000307          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTNP010000307 DAJTNP010000000
VERSION     DAJTNP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            14..655
                     /locus_tag="OO160_004333"
                     /pseudo
     CDS             14..655
                     /locus_tag="OO160_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          175 a          156 c          169 g          155 t
ORIGIN      
        1 tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac caggtggtca
       61 acaggagtgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct
      121 ggcacgtcag aatgagaatc atgaggccgg taataaatac atcccttacg ggaaaatggc
      181 ttcctggctg gttgagtgga aaaatgccac tgaaacgcaa tggcttaaag attctccctc
      241 acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact tcttccggaa
      301 gcgggctgct tttccccgat tcaaaaagcg gtgacagaat gatgcattcc gctacccgca
      361 gggcgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaactgg gctggatgcg
      421 ctaccggaat agccgtcagg tcacgggtgt tgtgaaaaat gtcactgtca gccagtcctg
      481 cggtacgtgg tacatcagta ttcagacaga aagtgaagta tccactccgg ctcacccttc
      541 agcatcaatg gtcgggctgg atgctggcgt ggctaaactc gccacgctgt cagatggcac
      601 agtctttgag cctgtaaaca gttttcagaa aaaccagaag acgctggcga gactt
//
LOCUS       DAJTNP010000308          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTNP010000308 DAJTNP010000000
VERSION     DAJTNP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(24..>649)
                     /locus_tag="OO160_004334"
     rRNA            complement(24..>649)
                     /locus_tag="OO160_004334"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJTNP010000309          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTNP010000309 DAJTNP010000000
VERSION     DAJTNP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..545)
                     /locus_tag="OO160_004335"
     CDS             complement(<1..545)
                     /locus_tag="OO160_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5526169.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGL"
BASE COUNT          147 a          159 c          168 g          169 t
ORIGIN      
        1 agaccagagg tatcaccagt gtcgactctc tgctgtgctg cggtcaggac ttccagcgct
       61 aaccttatgc ccttctgctg gtccagactc aaggcttgcg tagcggctag ctccttcagg
      121 ttggacttct cgtcgaccag agtagcgacc tcaagtccat tccagtaatc gtcactagcg
      181 tctgtcagga actgctcagc catagaccgg agatgctctg taagctcctg tagtgagact
      241 tcaggcctat ccaacatgaa ggccttagcg gtggctgcaa gctctctctg acgcattgtg
      301 gctactctcc tgtctgtgct gcgggtcgat actgaaagaa acggtgtggt ctgtcccttg
      361 agtctgaagc ggatagtata tcgaccattg gctctgcgga ctaactgagg cttagctacg
      421 ctgagtagat tgatttgtgt cagccttgtg tcagggcttg aggtgtagac ttgctgatac
      481 cgatagggag acccagagaa agaactggat ggatatacag tgttatcatg attaatgtca
      541 gtcatatcag gaccttaagt agcattgagt caactacttc aagtccatcc atccgtctgg
      601 caatggagtc catctggaac tacgcgggaa ataccctcgt taa
//
LOCUS       DAJTNP010000310          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTNP010000310 DAJTNP010000000
VERSION     DAJTNP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..635
                     /locus_tag="OO160_004336"
                     /pseudo
     CDS             1..635
                     /locus_tag="OO160_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          162 a          182 c          171 g          120 t
ORIGIN      
        1 aacagtatca accagtaaaa aaccccgtaa acagcattcg cctgaatttc gcagtgaagc
       61 cctgaagctt gctgaacgca tcggtgttac tgccgcagcc cgtgaactca gcctgtatga
      121 atcacagctc tacaactggc gcagtaaaca gcaaaatcag cagacgtctt ctgaacgtga
      181 actggagatg tctaccgaga ttgcacgtct caaacgccag ctggcagaac gggatgaaga
      241 gctggctatc ctccaaaagg ccgcgacata cttcgcgaag cgcctgaaat gaagtatgtc
      301 tttattgaaa aacatcaggc tgagttcagc atcaaagcaa tgtgccgcgt gctccgggtg
      361 gcccgcagcg gctggtatac gtggtgtcag cggcggacaa ggataagcac gcgtcagcag
      421 ttccgccaac actgcgacag cgttgtcctc gcggctttta cccggtcaaa acagcgttac
      481 ggtgccccac gcctgacgga tgaactgcgt gctcagggtt acccctttaa cgtaaaaacc
      541 gtggcggcaa gcctgcgccg tcagggactg agggcaaagg cctcccggaa gttcagcccg
      601 gtcagctacc gcgcacacgg cctgcctgtg tcaga
//
LOCUS       DAJTNP010000311          634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTNP010000311 DAJTNP010000000
VERSION     DAJTNP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..157
                     /locus_tag="OO160_004337"
     CDS             <1..157
                     /locus_tag="OO160_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5526170.1"
                     /translation="STPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKER
                     RKEWGTL"
     gene            142..>634
                     /locus_tag="OO160_004338"
     CDS             142..>634
                     /locus_tag="OO160_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5526171.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVR"
BASE COUNT          172 a          136 c          185 g          141 t
ORIGIN      
        1 cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga atgatttctg
       61 gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt acctgcagcg
      121 tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact tggcaacatg
      181 attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc tctgaatcat
      241 ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga ctgtgtgttg
      301 tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat ggcactaggt
      361 gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc ggtggtggtg
      421 tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt actggggcgg
      481 gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat taatccggag
      541 agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc cagggggaaa
      601 caccagatca ctttaatgtc tgatattgtc aggg
//
LOCUS       DAJTNP010000312          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTNP010000312 DAJTNP010000000
VERSION     DAJTNP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>628)
                     /locus_tag="OO160_004339"
     CDS             complement(<1..>628)
                     /locus_tag="OO160_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526172.1"
                     /translation="SVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSR
                     GFTKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSL
                     SNGSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGC
                     TAKNKFIPRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
BASE COUNT          158 a          181 c          126 g          163 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgac
//
LOCUS       DAJTNP010000313          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTNP010000313 DAJTNP010000000
VERSION     DAJTNP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            119..>620
                     /locus_tag="OO160_004340"
     CDS             119..>620
                     /locus_tag="OO160_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5526173.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAG"
BASE COUNT          125 a          172 c          186 g          137 t
ORIGIN      
        1 tcgcccgctg cacggagcgg gaatttgcga ccattcttgc cggtatcagc gccggcggta
       61 tgtactgccc ggagtgtggc acggttcact ggccggacgg agtcgccccg cccttctgat
      121 gctcccccgt tttgccgata tttttcagca gggaaaccgc tggcttaact ggctggagaa
      181 acaaccggaa ggggcagtcc gtccggtggt aattgaatca gtgacaaaaa tcatggcctg
      241 cgggaccaca ctgatgggct acacgcagtg gtgttgttca tcaccggact gttgccatac
      301 aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg cactgcgggg tgaaggctgg
      361 cgcacagtgg atacagtatc tgctgagcct ggtcccctac tgcccgtggc agcatattgt
      421 gttcacactt ccctgccagt actggtccct ggtgttccac aaccggtggt tactggcaga
      481 gatgagccgc attgcagcgg atgtgatact ggaaatctgc catcaggcag atgtggagcc
      541 ggggatattc acggtgatcc acacatgggg gcgtgaccag cagtggcatc cgcatatcca
      601 tttatcgaca actgccggtg
//
LOCUS       DAJTNP010000314          599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTNP010000314 DAJTNP010000000
VERSION     DAJTNP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(166..>599)
                     /locus_tag="OO160_004341"
     CDS             complement(166..>599)
                     /locus_tag="OO160_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526174.1"
                     /translation="YLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFM
                     ERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLK
                     VDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          141 a          191 c          117 g          150 t
ORIGIN      
        1 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
       61 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      121 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      181 catccgggcc agcggctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      241 ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc agcgggtcaa ccttcagtaa
      301 cctctgatac atccctctcc aggtgatttg catcgccgtt tttctcactg tctccgttat
      361 gatgtacacc acttcttcca gtaaccgccg tttcgccgga ctcaaaaaac cgtagtacct
      421 caccatacgg aaccccttat ccgccacatg ccaggagaac ctttccatga actcatctcc
      481 actcatcaac aggtattctt cccgttttgt tcggtgactg ttgtaacgca gaccgatttc
      541 atcctgaccg gcataatgct ccagacgact catcggcacc ggtggctttt tcaggtaag
//
LOCUS       DAJTNP010000315          593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTNP010000315 DAJTNP010000000
VERSION     DAJTNP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT          184 a          141 c          116 g          152 t
ORIGIN      
        1 ttgtatgaaa aaacacgcac gactaattga caataatttt catttccatt aaaaaagatc
       61 gcgtagcatt cttaattcat gaggaacttt acccgccagc aatctgagta gcgaaagctg
      121 ttcatcccca atgtttttgc tggcgggtcc ttttttctac ggtcctcctc cccggagggg
      181 ggcatttcat tatttttcta atcgttactg gtgagaaggc ggaagaaaaa gccagttctt
      241 cgcccctaaa taacagcatg attccagtcc atgcaaaatc tgatttccag ctcacacata
      301 ccgagcactg tacaaaaaac cgccagccaa ggcagttaag agtgtgttcc cggggtttgc
      361 ttagaatatt ttgtaagttg tccgaagaga tatttacaac accagaatga tgaatcatca
      421 gtcccctgcc agaggttcac tacgtaacct gaccgacaat gcaccattct ggtgctgaaa
      481 aaagagcact ggaactgcaa caaaacaaca tcacagaaca ggagatggta aggagtcaga
      541 cattcccaca caatattgtg tcagtgcaaa taacaacctt cgtctcaaat ctc
//
LOCUS       DAJTNP010000316          580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTNP010000316 DAJTNP010000000
VERSION     DAJTNP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT          209 a          104 c          122 g          145 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 gggggggatg aaaatacctg aagcagtaaa tcatattaat gttcagaata atattgatct
      541 tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
//
LOCUS       DAJTNP010000317          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTNP010000317 DAJTNP010000000
VERSION     DAJTNP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(59..>579)
                     /gene="treF"
                     /locus_tag="OO160_004342"
     CDS             complement(59..>579)
                     /gene="treF"
                     /locus_tag="OO160_004342"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR5526175.1"
                     /translation="FRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIVP
                     LYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKM
                     YGDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNG
                     VVRRLIGLYGEP"
BASE COUNT          132 a          169 c          141 g          137 t
ORIGIN      
        1 cctcctgaag aggataattt tacagccagc aggaagttag cggctggctg taaaaatatt
       61 atggttcgcc gtacaaacca attaaacggc gtaccacacc gttagtccag ccaaacccat
      121 cctgcaacgg atactcgcca ccgccgcctt cgcggggaac accatcggca atatggtatt
      181 tctcgatcat tttgtgctgt tccagataga actgattcac cgttttcagc cagctgcgcg
      241 cgatttcatc acccagaagg tcatcgccat acattttaaa tccctgaatt gccatccatt
      301 gtaacggtgc ccagccgttg ggtttatccc actgttcacc ggtttcgtac tcgcttgcca
      361 aaatcccgcc aggtgtcagt aaccggctgc gtacggcgtt tgccagacga tcggcctgtt
      421 catggttcgc catgccgaca tagagcggca caatggcggc agcggaaaac agcgccagtt
      481 gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc atcatcccag aggtaacggt
      541 ttaccgcatc gcgacgggca ctggccttct ggcggaaca
//
LOCUS       DAJTNP010000318          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTNP010000318 DAJTNP010000000
VERSION     DAJTNP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>571
                     /locus_tag="OO160_004343"
     CDS             <1..>571
                     /locus_tag="OO160_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5526176.1"
                     /translation="SMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVI
                     SMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNP
                     RKIFISASGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPIS
                     AFDVLISDRPLPADYVTHCQNGSVKIITPD"
BASE COUNT          144 a          139 c          122 g          166 t
ORIGIN      
        1 atccatgcca gtaatccatg acgttccaaa aaatcatcgt gatgacttac ctattgcaat
       61 tctggctgcc ggaatggtta atgaaaatga tctgatcttc tttgataatg gccaggagat
      121 accactcgtt ataagcatga tcccggatgc aatcaccttc accggcatct gttactcaca
      181 tcgcgtcttt gttgcgttga atgaaaagcc taatgtaaca gcaatacttt gtggtggtac
      241 gtatcgtgcc agaagtgatg ctttttacga tgccagtaac tcttcgccat tagactctct
      301 caatccgcga aaaatattta tttccgccag cggtgtgcat aatcactttg gcgtcagctg
      361 gtttaaccct gaagatcttg ccactaagcg taaagtgatg aaccgtggac tacggaaaat
      421 tttgctcgcc cgccacgcgt tgttcgatga agtggcctct gccagcctcg caccgatctc
      481 tgcatttgac gttctgatta gcgatcgtcc gttaccggca gattatgtta cgcactgcca
      541 gaatggttct gtaaagatca ttacacctga t
//
LOCUS       DAJTNP010000319          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTNP010000319 DAJTNP010000000
VERSION     DAJTNP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            129..>550
                     /locus_tag="OO160_004344"
     CDS             129..>550
                     /locus_tag="OO160_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR5526177.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQ"
BASE COUNT          162 a          135 c          125 g          128 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa
//
LOCUS       DAJTNP010000320          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTNP010000320 DAJTNP010000000
VERSION     DAJTNP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            44..>533
                     /locus_tag="OO160_004345"
     CDS             44..>533
                     /locus_tag="OO160_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526178.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLP"
BASE COUNT          123 a          148 c          128 g          134 t
ORIGIN      
        1 catttaaacc gtctggtctg tttcctccgg ctctacaaaa ataatgtcca tcatttttaa
       61 tggacactat cgtatgaaac accggacctg gatcactgaa gctttacgtc ttcactttga
      121 agaacattta ccccgggttg tggccgggcg tcgcctgggt gtaccaaaat caacagtttg
      181 tagtatgttt gtgcgctttc ggagagctgg cctttcgtgg cctttgccct caggcatgtc
      241 ggagcaggaa cttgatgcct gcctttacgg acaattttcc acggtaccag tcgtacgtcc
      301 tgaaagcacc gttatatccg aagcccccgt ggtaaaaaaa cgtccccggc ggcccaactt
      361 cccttatgag tttaaaatcg ccttagtgga gcagtcactg cagcccggag cctgtgtggc
      421 gcagatcgcc cgggaaaacg gaatcaacga taacctgctc ttcaactggc gccatcaata
      481 ccggaaaggt ggcctgctgc cttccggaaa aaatatgccg gcactgcttc ccg
//
LOCUS       DAJTNP010000321          521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTNP010000321 DAJTNP010000000
VERSION     DAJTNP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(213..521)
                     /locus_tag="OO160_004346"
                     /pseudo
     CDS             complement(213..521)
                     /locus_tag="OO160_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
BASE COUNT          162 a          106 c          114 g          139 t
ORIGIN      
        1 tgcagctcac cagtaaaatc agcaatcaga gaaggaattg ccttattcat accagggcaa
       61 ggtattaatt taaactgtaa taatttaatt ttaggatgtg tagcagcagc ccgatacaga
      121 gttgcaagga cacacttttg ccagagggcg ttacaggaaa gcttaatgtt tgattctgta
      181 tacataataa atcaccttat agttacaata ggtcaaaaac cgctgtagcc aaagttacgc
      241 tggcctgatg ctttagtgtc gagcttcgtc aaataatcca gacgtaccaa taagcgctgg
      301 tactgctctg ggaaattagg atcgttaata tccaggatgt aacgtccatt agcagggaaa
      361 tgaactaatc cctggtgatc ttccagttcc aaccctaagg cactgtacca ggctccggta
      421 attaaccctc tcagggtgtc agagcgctca tagtttccca gatggtaata ggcgtctttg
      481 ttaaaaaaag gcagacgtga taatgacatt taaaggactg c
//
LOCUS       DAJTNP010000322          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTNP010000322 DAJTNP010000000
VERSION     DAJTNP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>519
                     /locus_tag="OO160_004347"
     CDS             <1..>519
                     /locus_tag="OO160_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5526179.1"
                     /translation="PRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLITSFIGNGP
                     VRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTHTRWDKKF
                     TSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQISEELGFHYLYTT
                     ERRMNAPAKGTTR"
BASE COUNT          169 a           88 c          108 g          154 t
ORIGIN      
        1 ccccgtaaaa gcgttatgtt gacatttgat gatggatacc ttgacaactg gtttcaggta
       61 tatcctttat taaacgagtt taatctcaag gcacatattt ttttaattac cagttttatt
      121 ggaaatggtc cggttcgtca ctctcctgga aaggaatatt ctcatcgcga ttgcgaacat
      181 caaatagcaa ccgggaatgc tgataatgtt atgcttcgct ggtctgaagt gaacgaaatg
      241 ttgcaatctg gtctggtcga atttcatgtt catacacata ctcatacacg ttgggataaa
      301 aagttcactt cacgagaaga acagtgtaaa catcttcggc aggacttatt gagcgggaga
      361 gaatatttaa aaaaaatgac aggaaaatgc agtaaacatt tgtgttggcc ggaaggttat
      421 tataacaagg attatattca gatctctgaa gaacttggat tccattattt atatacaaca
      481 gaaagaagaa tgaatgctcc tgctaaagga actacacgg
//
LOCUS       DAJTNP010000323          515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTNP010000323 DAJTNP010000000
VERSION     DAJTNP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>515)
                     /locus_tag="OO160_004348"
     CDS             complement(<1..>515)
                     /locus_tag="OO160_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5526180.1"
                     /translation="EKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSP
                     ADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPV
                     AQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQA
                     KDISHLLAFTC"
BASE COUNT          136 a          123 c          126 g          130 t
ORIGIN      
        1 gacaagtaaa agccagcaaa tgactgatat ctttagcctg aggatcaacc gagaggcgag
       61 aagaaacgct gtatgcccca gatgcctgtt cacgaatatt aacgcgtaga tcttttgcca
      121 gtgcgacgtt aaaagcatcg agcgccatac gcgtcgccag attaacaggt gtccgggaat
      181 cataacgctt ccactgtgaa acctgtgcca caggttcatt ttgttctttt acagtaaccg
      241 atgcgttgtc cgtcgcgcga gttaatggtt tacctgcggc taatggcgaa tcagagtgtt
      301 tgattgagcc taagtaacgc gtaattaacg ccacgagttt gtcttctgcg acattaccga
      361 caatgacaaa cgtgatatcc gctggagatg aaaacaactg gcgatcggca gccagcgcat
      421 ctgcggcagt aaactgtgca atctgatttt cttgcagtaa tttcgtgcgg tcatcagcat
      481 agcgcgtctc atacatcagc tgggcgaatt tctcc
//
LOCUS       DAJTNP010000324          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTNP010000324 DAJTNP010000000
VERSION     DAJTNP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(35..307)
                     /locus_tag="OO160_004349"
     CDS             complement(35..307)
                     /locus_tag="OO160_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526181.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          116 a          128 c          143 g          127 t
ORIGIN      
        1 tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca ccacttcggg tcatccgcct
       61 tcatctccgc ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt
      121 attcgtacgg tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg
      181 agggatgacc ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg
      241 cgacggcttt cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt
      301 gtgacatggg aactcattct ggatggttac tctgaaagct catattctgc cacaccccga
      361 tttgcagccg ccaggctgcc gtggttcaca gataaaacac tctccaggaa acccggggcg
      421 gttcactgcg tgtctgggtt cgccagcatg agcgggatac cgggggcggt tcacacgaac
      481 ataccacaga cagttgattt tggtacgccc aggc
//
LOCUS       DAJTNP010000325          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTNP010000325 DAJTNP010000000
VERSION     DAJTNP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>511
                     /locus_tag="OO160_004350"
     CDS             <1..>511
                     /locus_tag="OO160_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5526182.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQ"
BASE COUNT          121 a          156 c          141 g           93 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca a
//
LOCUS       DAJTNP010000326          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTNP010000326 DAJTNP010000000
VERSION     DAJTNP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            71..352
                     /locus_tag="OO160_004351"
     CDS             71..352
                     /locus_tag="OO160_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5526183.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..>504
                     /locus_tag="OO160_004352"
     CDS             318..>504
                     /locus_tag="OO160_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5526184.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPA"
BASE COUNT          181 a          105 c          108 g          110 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctg
//
LOCUS       DAJTNP010000327          498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTNP010000327 DAJTNP010000000
VERSION     DAJTNP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..498
                     /locus_tag="OO160_004353"
                     /pseudo
     CDS             1..498
                     /locus_tag="OO160_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          141 a          133 c          129 g           95 t
ORIGIN      
        1 ggaaaaacac caaaagcctc ccccattacc ccggaacaaa ttgaaatccg tgagctcagg
       61 aaaaagctac aacgtattga aatggaaaat gaaatattaa aaaggctacc gcgctcttga
      121 tgtcagactc cctgaacagt tttcgataat cgggaaactc agggcgcgtt atcctgtggc
      181 cactctctgc catgtgttcg gggtccatcg cagcagctac aaatactgga aaaaccgtcc
      241 tgaaaaacca gacggcagac gggctgtatt acgcagccag gtacttgaac tgcatggcat
      301 cagccacggc tctgccggag caagaagcat cgccacaatg gcaacccaga gaggctacca
      361 gatggggcgc tggcttgctg gcagactcat gaaagagctg gggctggtca gttgccagca
      421 gccgactcac cggtataagc gtggcggtca tgagcacgtt gctatcccga atcatcttga
      481 gcgacagttc gccgtaac
//
LOCUS       DAJTNP010000328          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTNP010000328 DAJTNP010000000
VERSION     DAJTNP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(212..>497)
                     /locus_tag="OO160_004354"
     CDS             complement(212..>497)
                     /locus_tag="OO160_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5526185.1"
                     /translation="EWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVK
                     LSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          108 a          165 c          111 g          113 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacggaggc ccgcccccgg gcctcccctg attcatccgt ttccctgttc
      121 agcctgacag cacgccccgg ctgccggatg acagactccg cttcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attactcaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagaccgctc agctcctctt tcatttcacg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tcggcatcgc tccacgccat atgtgtttgt gctgtgacag
      421 attttgccag catgactacc ggggacaggc ggcccagtga gtcggggcca gcattccaga
      481 tacgaccgtt ccattca
//
LOCUS       DAJTNP010000329          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTNP010000329 DAJTNP010000000
VERSION     DAJTNP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>485)
                     /gene="capU"
                     /locus_tag="OO160_004355"
     CDS             complement(<1..>485)
                     /gene="capU"
                     /locus_tag="OO160_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5526186.1"
                     /translation="SKIAFEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIV
                     CHSGHDSNIVGLVRFFTWKHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHL
                     EQEGCRTRVTVVPPGFDFQELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGH
                     E"
BASE COUNT          149 a          110 c           92 g          134 t
ORIGIN      
        1 aattcgtgcc ctttttccgg gcgcaacatt cctacctgag caattacggg gcatcctctt
       61 cgggacgcca gccaggaaag aacactcgga ggcaaactgt ttcgggaatc aacgtataat
      121 tcctggaaat caaagccagg aggcacaaca gtaacccgag ttcgacatcc ttcctgctcc
      181 agatgtgtct tcatatttgt tccgggaaca atcacctcat cgcaaaaatg atttattgaa
      241 aaaaattttg tttttcgtgt caaatatgtc ttttgcctga taattctgaa tggatgtttc
      301 caggtaaaaa accgtaccaa accaacaata ttactatcat gtccactgtg acaaacgatt
      361 gcatcgggct gaaaactatg aactattccg agtaatctcc atacagtagg aatgtgaaga
      421 ctgtttcgaa ataacgcgaa tgtgatatca attccaaact tactggcttc aaaagcaatt
      481 ttgct
//
LOCUS       DAJTNP010000330          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTNP010000330 DAJTNP010000000
VERSION     DAJTNP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            120..>483
                     /locus_tag="OO160_004356"
     CDS             120..>483
                     /locus_tag="OO160_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5526187.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRL"
BASE COUNT          165 a          104 c          117 g           97 t
ORIGIN      
        1 gatcccacta agaataaatc tccttacctc acaacacgac aagcctgcat tgcggctctt
       61 cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg gcataacaga ggaaagaaaa
      121 tgtcactctt ccgcagaggt gaaatatggt acgccagttt cacattgccg aacggtaaaa
      181 gatttaaaca gtctcttgga acaaaggaca aaaggcaggc gacagaactc catgacaagc
      241 taaaggctga agcatggcgg gtcagcaaac ttggtgaaat acctgatata acgttcgagg
      301 aagcgtgtgt caggtggctt gaagagaaag cacataaaaa atcactggac gatgacaaaa
      361 gccggatcgg attctggctt caacatttcg caggaatgca actaagagac attactgaat
      421 caaaaattta ttcagcaatg cagaaaatga cgaaccggcg tcatgaggaa aactggagac
      481 tca
//
LOCUS       DAJTNP010000331          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTNP010000331 DAJTNP010000000
VERSION     DAJTNP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>479)
                     /locus_tag="OO160_004357"
     CDS             complement(<1..>479)
                     /locus_tag="OO160_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5526188.1"
                     /translation="AAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFSKDA
                     NQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLMGRS
                     EEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAMKKG
                     "
BASE COUNT          132 a          122 c           82 g          143 t
ORIGIN      
        1 cccttcttca tagcatcaaa taattcctgt tcagtctttc cggttgcttt agcgaatacc
       61 tgaaccatac caggaacagc ttcagctagt tgccccttaa gctcttcagc cataatctga
      121 cccttaccag ccatctgtga taatgctgtc agtgctcgct taatctcttc ttcacttcgc
      181 cccatcagac gagaatagcc aatcatattt ctaaaaagtt cttcagaacc ttgaatgcct
      241 agagactctc tagctgaagc accgaaacca gcaaactgtg attgcagttc agcgatatct
      301 aaacccgttg tttgagctaa tgactttgtg aaatctttta cttgatttgc atctttagaa
      361 aatacgaaat caattgaacg ttcagcactt gcacgttgaa ggccaacttc cattactcgc
      421 tgataagcgg atagtacagc ctgaaagctg ataagtacac ctgctgtttt aactgctgc
//
LOCUS       DAJTNP010000332          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTNP010000332 DAJTNP010000000
VERSION     DAJTNP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT          138 a          110 c          103 g          128 t
ORIGIN      
        1 tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt cactttaaaa
       61 agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc aacatggagt
      121 gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg tcctcaacgg
      181 ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg cctgctttta
      241 actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg tataatctga
      301 ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcatc tctcaaagag
      361 actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt tgatcgcgct
      421 gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc atctcattg
//
LOCUS       DAJTNP010000333          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTNP010000333 DAJTNP010000000
VERSION     DAJTNP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..205
                     /locus_tag="OO160_004358"
     CDS             <1..205
                     /locus_tag="OO160_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526189.1"
                     /translation="SNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLPASGKYELR
                     VLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(313..>474)
                     /locus_tag="OO160_004359"
     CDS             complement(313..>474)
                     /locus_tag="OO160_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526190.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          152 a          115 c           83 g          124 t
ORIGIN      
        1 ctctaatgaa ggcgctgata cttatctctt cgggccagga atcgatgatt ctgttgattt
       61 atccagatat tccccagaac tcgatagtca cggacaatac tcactccctg cttctggcaa
      121 atatgagctg cgagtgctgc aaactcgaaa cgacgcccgt aaaaacaaaa ctaaaaaata
      181 taacgtcgat attcaaatta agtaaatgtc aaccgacggg gactatcccc gtcatagacg
      241 ttaacgcccc actcctctct gtcgctcaag aggttaatga cgaatagcag agaacataat
      301 gtaaacaagg catcactcac gacacataaa taacatcttt gtggtggttt gattgatttt
      361 tccactccgc ttaccatcta caccaaaata acgatcatca cgacataatt tttctttaac
      421 atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaa
//
LOCUS       DAJTNP010000334          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTNP010000334 DAJTNP010000000
VERSION     DAJTNP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>470)
                     /locus_tag="OO160_004360"
     CDS             complement(<1..>470)
                     /locus_tag="OO160_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5526191.1"
                     /translation="HDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVI
                     EGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPR
                     RREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT           93 a          141 c          123 g          113 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg
//
LOCUS       DAJTNP010000335          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTNP010000335 DAJTNP010000000
VERSION     DAJTNP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>470
                     /locus_tag="OO160_004361"
     CDS             <1..>470
                     /locus_tag="OO160_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5526192.1"
                     /translation="HDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVI
                     EGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPR
                     RREDYGKDLWSTYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT          108 a          124 c          144 g           94 t
ORIGIN      
        1 catgacggta cctccagcta ccagatgctg ccgggttact tcagattcgt ctgccagaac
       61 gggtgtgtct gtggtcagtc tctgggggaa gtgcgtgttc cgcaccgggg aaacgtggtg
      121 gagaaagtta ttgaaggggc ttacgaagtg gtgggcgtgt ttgaccggat tgaggagaaa
      181 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
      241 gcactgactt accgttatgg tgacgaacat cagcccgtca ccaccgccga cattctgacg
      301 ccacgacgcc gggaggatta cggtaaggac ctgtggagta cttatcagac catccaggag
      361 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
      421 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt
//
LOCUS       DAJTNP010000336          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTNP010000336 DAJTNP010000000
VERSION     DAJTNP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT          131 a           97 c           86 g          156 t
ORIGIN      
        1 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
       61 gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt tcagggaagt
      121 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttgctactaa
      181 atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg gttatatcca
      241 catagagaca aaaatattaa tctgataaat atttatttca cgccgtatat ttgattgttt
      301 aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata tatggttatt
      361 tttcaccctg cgtattcagc acgcaacaaa aaacccgccg aagcgggttt agtgcgggtg
      421 cgttgaggat gcctgacaca tgagaggtgg cgagggattt ctcccccgcc
//
LOCUS       DAJTNP010000337          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTNP010000337 DAJTNP010000000
VERSION     DAJTNP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>463
                     /locus_tag="OO160_004362"
     CDS             <1..>463
                     /locus_tag="OO160_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5526193.1"
                     /translation="EITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHPVIT
                     GHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPEI
                     AINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGG"
BASE COUNT          120 a           99 c          116 g          128 t
ORIGIN      
        1 gaaattaccg ataatgaact gttagtgagt gtaatttctg acagcgtctg tttatcgacc
       61 tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac ccgacgattt agaaaatcat
      121 ccggtcatta ccgggcatga atgtgccggg gttattgtcg aagtgggtaa aaatctcact
      181 ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt accaagcgga
      241 tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat gattattccc
      301 gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg
      361 tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta tcacaccacg
      421 caatatgttt atgagcatcg catgggcgtc aaacctggcg gca
//
LOCUS       DAJTNP010000338          462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTNP010000338 DAJTNP010000000
VERSION     DAJTNP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            287..>462
                     /locus_tag="OO160_004363"
     CDS             287..>462
                     /locus_tag="OO160_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel"
                     /protein_id="HCR5526194.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDL"
BASE COUNT          131 a           92 c           87 g          152 t
ORIGIN      
        1 atttaagggc acggtttttg caaccgtgtt cttgtttggc ctttccttat agccggtcag
       61 tgcattcact accacaagca gataataact ttatctgtat ttctttatat gatgtgagca
      121 tgttcaggaa aaaagccctt tcctgacgca gagttgctca ctcaaaacct ttgattactg
      181 cggataaagt catctactat tgtttaacac attcacacgc tgcatgtatg ttcagaaaat
      241 gtgcacgcta agataattaa acaaaattgt ggttacagag gtatcagtga gtcactgggc
      301 tacattcaaa caaacagcaa caaatttatg ggtgacccta cggcacgaca ttctcgcgct
      361 ggccgtcttt ttaaatggat tgcttatttt taaaacaatc tatggtatgt cggtcaattt
      421 gcttgatatt ttccatatca aagcattttc agagttggat ct
//
LOCUS       DAJTNP010000339          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTNP010000339 DAJTNP010000000
VERSION     DAJTNP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..139
                     /locus_tag="OO160_004364"
     CDS             <1..139
                     /locus_tag="OO160_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526195.1"
                     /translation="AGDPGRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRDKTR
                     E"
     gene            136..>454
                     /locus_tag="OO160_004365"
     CDS             136..>454
                     /locus_tag="OO160_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5526196.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          137 a          113 c          123 g           81 t
ORIGIN      
        1 ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc tcagttatcc atgctactgg
       61 aggggatcgc gtggcaacat cccaaacgga cagaacggcc tggcatccgg atataacccg
      121 tgataaaaca agggaatgaa caatgaactc cccgatgata ttgagctgct taaagccatg
      181 ttgcgtaagc aacagagtcg gcttcgacag tatgcctgtc aggtcgcggg ctatgagcag
      241 gaaattgaac ggctgaaagc gcaactcgac aggttgcgtc gtatgttgtt cggccagagt
      301 tcagagaaaa agcgtcataa gcttgaaaat cagatccgac aggcagaaaa acgactgtcg
      361 gaactggaaa accggctgaa cacagccaga aatcttctgg aagatgcatc gtcagtcaca
      421 gattcacctg acaccagtcc cccgtcagaa aacc
//
LOCUS       DAJTNP010000340          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTNP010000340 DAJTNP010000000
VERSION     DAJTNP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            1..439
                     /locus_tag="OO160_004366"
                     /pseudo
     CDS             1..439
                     /locus_tag="OO160_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          107 a          121 c          105 g          114 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgc
//
LOCUS       DAJTNP010000341          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTNP010000341 DAJTNP010000000
VERSION     DAJTNP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..430)
                     /locus_tag="OO160_004367"
     CDS             complement(<1..430)
                     /locus_tag="OO160_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526197.1"
                     /translation="MAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAEL
                     GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSP
                     GSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPL"
BASE COUNT          112 a          117 c           98 g          117 t
ORIGIN      
        1 gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg gtatccgata
       61 tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa acggtgttac
      121 tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc tctgttcgat
      181 atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg ttaacagtga
      241 acactatgct gttatccggg tttctctggc tgaaaaaatg ccagcctgaa tcatcaccaa
      301 gctctgcaac aacaggcctg gatggattac cccacaaata aaacgctgca tttttcgtgg
      361 agttgttggc gctggataac gtgaattttc tggcagttcc ggcctgaata ttcttaaaag
      421 caatagccac tccattctga aagc
//
LOCUS       DAJTNP010000342          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTNP010000342 DAJTNP010000000
VERSION     DAJTNP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..161)
                     /locus_tag="OO160_004368"
     CDS             complement(<1..161)
                     /locus_tag="OO160_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5526198.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLG"
BASE COUNT          161 a           71 c           71 g          133 t
ORIGIN      
        1 ttacccagtg ggacaatttg atcggctgta tcggaggtaa cttcacaagt agtgtcgaca
       61 atttcaccga cgaagttaac aacaccatct tctgccagtg catgcccggc cagcattaga
      121 gatgccagag tgaatgcacc gcctataata ttacgcttca taaaaactcc attgatataa
      181 tatatccttt accatgaatc atcacttata ttaataagtg tgtttttgat tcaaaggaat
      241 agtacaccaa agagacggct ttatctaaaa ataatattaa ttacgcaaaa ataaaatata
      301 gatttattgt ttaaatatta tttaaacagt aaaaaatcaa gtttgttatc agacattaat
      361 gataattcag aatatattta tatagaaaga tagatgaatt tattgatgtc agttataaaa
      421 aagggccacc ggtcgg
//
LOCUS       DAJTNP010000343          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTNP010000343 DAJTNP010000000
VERSION     DAJTNP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            35..434
                     /locus_tag="OO160_004369"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO160_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTNP010000344          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTNP010000344 DAJTNP010000000
VERSION     DAJTNP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(151..420)
                     /locus_tag="OO160_004370"
     CDS             complement(151..420)
                     /locus_tag="OO160_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526199.1"
                     /translation="MLETDQAWDVFEKLEDCYFSQRDPSTPVSCQKSYDTRVLCYQRG
                     GVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVERLMQL"
BASE COUNT          116 a          113 c           85 g          119 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgt
//
LOCUS       DAJTNP010000345          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTNP010000345 DAJTNP010000000
VERSION     DAJTNP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>428
                     /gene="istA"
                     /locus_tag="OO160_004371"
     CDS             <1..>428
                     /gene="istA"
                     /locus_tag="OO160_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HCR5526200.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT          100 a          118 c          105 g          105 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcg
//
LOCUS       DAJTNP010000346          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTNP010000346 DAJTNP010000000
VERSION     DAJTNP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..305)
                     /locus_tag="OO160_004372"
     CDS             complement(<1..305)
                     /locus_tag="OO160_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5526201.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          122 a           80 c           91 g          133 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttgttaatgg ataaatccat caacttgtgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggccaggt tctgacgagc gttgccatca agatataatt catcattgat aatctgaaat
      181 gcgacatcgt cgcgcatttc gtgcagcgga aaacgttttg attctgcgat agtggaaata
      241 gacttcgcac caaaacgtga atcgagtagt tccgacctta aatccgttac ttgcttctta
      301 tccattttaa actccttaaa atgattggat cgcattaaaa aagtaggatt tatcgataaa
      361 gtaagcaagt gcaaaggact cgtgtttaaa taacaaaatc ctaatgttat ttatcgtgag
      421 atattg
//
LOCUS       DAJTNP010000347          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTNP010000347 DAJTNP010000000
VERSION     DAJTNP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(312..>420)
                     /locus_tag="OO160_004373"
     CDS             complement(312..>420)
                     /locus_tag="OO160_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5526202.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          116 a           97 c           86 g          121 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcact cccgtaaaga caggagtcaa
       61 gttcccgctc cgacataact gcgtgcaatg gattttgaag gaaccgtcag cccattcccc
      121 ggaggcaaac atgggctggc aaaagtgtag cggtattagg cacagctatt taggaaagag
      181 ggaccatctc catctcatta aatggaccac gccagacaat tcactgtcat gctgatgctg
      241 cactgtgggg acagaatcac tcatgatgac ttcgataatc gacgacatta ttatttggcc
      301 gagcttttag gtcatatctc aaatcgtttg cctaatattg ctccattttt taataaaaaa
      361 tcaatcattt ttgaatcttt gcttttcatt gcattgtcaa gcatagtgtc cccactattt
//
LOCUS       DAJTNP010000348          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTNP010000348 DAJTNP010000000
VERSION     DAJTNP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>419
                     /locus_tag="OO160_004374"
     CDS             <1..>419
                     /locus_tag="OO160_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5526203.1"
                     /translation="APAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTD
                     KPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGV
                     TYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG"
BASE COUNT           97 a          117 c          121 g           84 t
ORIGIN      
        1 gcgcctgcgc cgaaaaatgg tcaggccgtt ggcaccaaca ccattggttc ttccgaggcc
       61 tgtatgctcg gcgggatggc gatgaaatgg cgttggcgca agcgtatgga agctgcaggc
      121 aaaccaacgg ataaaccaaa cctagtgtgc ggtccggtac aaatctgctg gcataaattc
      181 gcccgctact gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg
      241 gacccgaaac gcatgattga agcctgtgac gaaaacacca tcggcgtggt gccgactttc
      301 ggcgtgacct acaccggtaa ctatgagttc ccacaaccgc tgcacgatgc gctggataaa
      361 ttccaggccg acaccggtat cgacatcgac atgcacatcg acgctgccag cggtggctt
//
LOCUS       DAJTNP010000349          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTNP010000349 DAJTNP010000000
VERSION     DAJTNP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..276)
                     /locus_tag="OO160_004375"
     CDS             complement(<1..276)
                     /locus_tag="OO160_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5526204.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKL"
BASE COUNT          100 a          114 c           96 g          106 t
ORIGIN      
        1 cagtttaaat gactcaccac cagaggtagc aatctcatgg gtggtgagac gcacagggtt
       61 gctacaaccg gtgactaccg aaaccggcca gccttgcggc tgccctgcac gccccaccat
      121 aatctgaatg tggctgtgct tgacgcataa aaaaaccgcc tgagcgcggt tatgcgcagt
      181 agtcatcgtc ggggtagcaa tcccggcacc cgttttatga ggtgcaggtg cactataatt
      241 ccacccgttc tggttttcaa tagctacatt caacattttc tctttccttt catcaccgaa
      301 gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac gttaaccagt taacaattac
      361 agttggcttt cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcg
//
LOCUS       DAJTNP010000350          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTNP010000350 DAJTNP010000000
VERSION     DAJTNP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT          169 a           62 c           61 g          118 t
ORIGIN      
        1 ctgcagcaat aattttcatt attgcaatct cctgtgttgt aaatagacgt ttcaaaccta
       61 attttacgcc tactaccata caagagagaa aaagcgatta ttcaaccgtg atcacattaa
      121 aaacaaaaat ttctaccgag gagaaaaaat accctcaaaa accacctgaa taattaacca
      181 ttattgttaa aacaacacgt tgaataatca cataacaaac tccattaatc attgaaaatg
      241 agcacagaaa tagaaattaa ttatattcaa ttaattaaaa tgtgtattgc ttgatgtatt
      301 taaaaatatt attttcatgg tgagagtgga ggtaaagaag acacaataat aaaaaggaat
      361 gtgacaaata atggttctgg ttgggcgtta aatttaaggt caccagaatg
//
LOCUS       DAJTNP010000351          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTNP010000351 DAJTNP010000000
VERSION     DAJTNP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            35..>406
                     /locus_tag="OO160_004376"
     CDS             35..>406
                     /locus_tag="OO160_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5526205.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNP"
BASE COUNT          124 a           91 c           93 g           98 t
ORIGIN      
        1 ttgctaataa ttaaattatt acaaaggtga tcaaatggaa tggcaacaat tgcttaactg
       61 caaccgtcgg aaagataatc taaaaaaaac caacatagca tttttacatc cccttgctag
      121 cggaagacaa gagatcgagc gggattacga ccgattactt tttgctgcac caactcgtcg
      181 gcttgcggat aaaacacaag ttttcccgtt agatcaaaat gacagcatac ggacgaggct
      241 gactcattct cacgaagtag caaactttgc tcgcgggata ggaatgaggc tcgctttcga
      301 gatgaaggag cacatctttg gcgaaatacc cacggggatt gtcgttgaac gcgacgttcc
      361 tgctctgtta gcagcgatcg gactggcaca cgatctcggt aatcca
//
LOCUS       DAJTNP010000352          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTNP010000352 DAJTNP010000000
VERSION     DAJTNP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>398)
                     /locus_tag="OO160_004377"
     CDS             complement(<1..>398)
                     /locus_tag="OO160_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5526206.1"
                     /translation="ELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGG
                     RFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSML
                     SALVVELVLSAGFLLVIHGATDKFAPAGFA"
BASE COUNT          123 a          124 c           87 g           64 t
ORIGIN      
        1 gcgcaaaacc tgccggagcg aatttgtcgg ttgcaccgtg gatcactaac aggaagcccg
       61 cactcaatac cagttcaact accagcgcgg aaagcatgga ataaccgcct ggtgaatgct
      121 cgccataacc gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg
      181 caattaaata cagcagcgcc gctgcaacaa taccgccgac aacctgggca attacgtagc
      241 caacgacttc ttttgccgga aaacgtccgc cagcccataa accaatagtg accgccgggt
      301 taaaatgacc accagaaata tgaccaacag cgaaggccat cgtcagaacg gtcaaaccaa
      361 atgccagcgc cacgccggca aaaccaatgc ctaattcc
//
LOCUS       DAJTNP010000353          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTNP010000353 DAJTNP010000000
VERSION     DAJTNP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..129)
                     /locus_tag="OO160_004378"
     CDS             complement(<1..129)
                     /locus_tag="OO160_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Involved in glycogen synthesis. May be involved in
                     glycogen priming; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthesis protein GlgS"
                     /protein_id="HCR5526207.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRT"
BASE COUNT          127 a           85 c           63 g          118 t
ORIGIN      
        1 agttctatgt tcttcatcca tgttaccagt aacagccaga atatcgaccg ggcgaccttc
       61 tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa ttattcaaag aattaagact
      121 atgctccata agcacatcct ccggttatcg catacctttt aaggccatat gctttttctg
      181 atttcagtat tccccaatca gtgtagtgcc tttaaacata acgtgcgtaa atatattgtc
      241 cccgatcaca ctttttagtg aaaaataaaa aaccatatta aaatactttc tcttccagaa
      301 cttctctaac atgtgttatt attaacaaag acaaatgtaa gcagggaaac tattccaagg
      361 taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DAJTNP010000354          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTNP010000354 DAJTNP010000000
VERSION     DAJTNP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..337
                     /locus_tag="OO160_004379"
     CDS             <1..337
                     /locus_tag="OO160_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526208.1"
                     /translation="TEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVR
                     GQSYRGSRLGPVGISVPGTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSGA
                     MPGLFIRTS"
BASE COUNT           96 a           93 c          106 g           94 t
ORIGIN      
        1 cacagaagat attatttaca gaatcgtggc gagctctgtc cgcaacagat ggggagaccc
       61 tgacattggc gggctgatta ttgctgcgta ccagggagaa gctgacggtg ataaagtcat
      121 cagacttgtc agggggcagt catacagagg ctcacgactg ggaccggtgg ggatttcagt
      181 gcccggtact cccaccggaa cgtatatagc atccccacaa tttttcatta cgggatgttc
      241 agagcattca ttaccggggt catattgcgc cctgtccggg gtgccggatg ctcatgtctc
      301 tggcgcaatg cccgggcttt ttattcgcac atcgtgagga atgcaccgtg gaaattaaaa
      361 aaatcattaa tccccgttat accgaaagt
//
LOCUS       DAJTNP010000355          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTNP010000355 DAJTNP010000000
VERSION     DAJTNP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..220)
                     /locus_tag="OO160_004380"
     CDS             complement(<1..220)
                     /locus_tag="OO160_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5526209.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQ"
BASE COUNT           89 a          100 c          103 g           95 t
ORIGIN      
        1 gttgtttcga ggcgatagtc gctggcagtt ccgggtaacc caggtgctca cgtccggcgg
       61 cgatcgcgcc cgacgtcaca ataacaatcc gatgcccggc ggcatgtaac tgcgcgcact
      121 ggcgaacaag ttcaacgata tgggcacggt tcagacggcg cgatccgcct gttagcacac
      181 tggtgccgag ttttaccacc agcgtctggc tgtcactcat gattctctgc cattcaattt
      241 taggaaaaat gatatcaaac gaacgtttta gcaggactgt cgtcggttgc caaccatctg
      301 cgagcaaagc atggcgtttt gttgcgcggg atcagcaagc gtagcggcag ttgtttacgc
      361 ttttattaca gatttaataa attacca
//
LOCUS       DAJTNP010000356          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTNP010000356 DAJTNP010000000
VERSION     DAJTNP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            260..318
                     /locus_tag="OO160_004381"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO160_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTNP010000357          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTNP010000357 DAJTNP010000000
VERSION     DAJTNP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..294
                     /locus_tag="OO160_004382"
     CDS             <1..294
                     /locus_tag="OO160_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526210.1"
                     /translation="NETDAEYLVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFD
                     ANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
BASE COUNT          125 a           74 c           95 g           79 t
ORIGIN      
        1 aatgaaacag atgctgaata cctggtgcgt aaattcgatg agcttgaagc taaatgtgca
       61 gcacaggaaa acaaagtaat accagtgtca actgaactgc caccagcaaa tgaaagtgtt
      121 ttgttattcg atgctaacgg agaaggctgg ctaattggct ggcgttctct ctggtacacc
      181 tggggacaaa aagaaaccgg agaatggcag tggacatttc aggtcgggga ccttgaaaac
      241 gtcaatatca ctcactgggc agtaatgcca aaagcaccgg aggctggagc ataatgacca
      301 cttttaccga caaagaactg attaaagaaa ttaaagagcg tatcagcagc ctcgacgtgc
      361 gagacgatat tga
//
LOCUS       DAJTNP010000358          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTNP010000358 DAJTNP010000000
VERSION     DAJTNP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT          100 a           86 c          112 g           67 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gggtgaaact ctgcggcctt tttcgtaaga aaaatgcaac gattgcacgc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagacg tgacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg
      241 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa catcacgggg gctaatcttg aacccaaaca actcaagaat agccgcgaaa
      361 cgttg
//
LOCUS       DAJTNP010000359          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTNP010000359 DAJTNP010000000
VERSION     DAJTNP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>365
                     /locus_tag="OO160_004383"
     CDS             <1..>365
                     /locus_tag="OO160_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5526211.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHW"
BASE COUNT           84 a           92 c          108 g           81 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctggg
//
LOCUS       DAJTNP010000360          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTNP010000360 DAJTNP010000000
VERSION     DAJTNP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT           77 a           89 c           86 g          111 t
ORIGIN      
        1 atccgatgtc aaatgttaac actctgcgag tggtacattt tccccggatt atcgtcctga
       61 gcctgccgct ggctctcttt ctaccgcctc gctttgctcg ttgctcaacg cctcacagac
      121 acggattaaa atccgcatcc gttcaccgtt ttttaaagtc cgttaaaagc atgatgccat
      181 ctccgagagt taatctcgtc aaatgctaaa tcgtgggggt cccctttggg gttccgattt
      241 agtgattgac gacaccaccg attaaaaaac ttatgcgggg tggatggttt cacgaagtga
      301 ggccatccac ctgtaagaca gggttttgtt tttattccct gttttggtga tcgggtgtgt
      361 gga
//
LOCUS       DAJTNP010000361          362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTNP010000361 DAJTNP010000000
VERSION     DAJTNP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>362
                     /gene="ydfJ"
                     /locus_tag="OO160_004384"
     CDS             <1..>362
                     /gene="ydfJ"
                     /locus_tag="OO160_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5526212.1"
                     /translation="VAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVM
                     VFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLI
                     QTFLAGYLVQTLLFNKAI"
BASE COUNT           85 a           80 c           97 g          100 t
ORIGIN      
        1 ttgtggctat gggaactaac tgcggcacct taagcgcaac ggcaatctgg gcctttatgt
       61 tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg ttcctggcga
      121 gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa agcccggtct
      181 ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct ggtagcatgt
      241 tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggcg ggtaactccg
      301 gtttaattca gactttcctt gcaggctatt tagtacagac gttattgttt aacaaagcaa
      361 tt
//
LOCUS       DAJTNP010000362          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTNP010000362 DAJTNP010000000
VERSION     DAJTNP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAJTNP010000363          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTNP010000363 DAJTNP010000000
VERSION     DAJTNP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>356
                     /locus_tag="OO160_004385"
     CDS             <1..>356
                     /locus_tag="OO160_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5526213.1"
                     /translation="KIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRLDS
                     IPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNVAG
                     NPLSGHTMRTLQQITT"
BASE COUNT          114 a           80 c           62 g          100 t
ORIGIN      
        1 caagataaaa gaattaccat ttcttcctga aaatctaact catttacggg ttcataataa
       61 ccgattgcat attctgccac tattgccacc ggaactaaaa ttactggtag tttctggaaa
      121 cagattagac agcattcccc cctttccaga taagcttgaa gggctggctc tggctaataa
      181 ttttatagaa caactaccgg aattaccttt tagtatgaac agggctgtgc taatgaataa
      241 taatctgaca acacttccgg aaagtgtcct gagattagct cagaatgcct tcgtaaatgt
      301 tgcaggtaat ccattgtctg gccataccat gcgtacacta caacaaataa ccaccg
//
LOCUS       DAJTNP010000364          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTNP010000364 DAJTNP010000000
VERSION     DAJTNP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            46..>353
                     /locus_tag="OO160_004386"
     CDS             46..>353
                     /locus_tag="OO160_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526214.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     V"
BASE COUNT           92 a           77 c           87 g           97 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggt
//
LOCUS       DAJTNP010000365          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTNP010000365 DAJTNP010000000
VERSION     DAJTNP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..119)
                     /locus_tag="OO160_004387"
     CDS             complement(<1..119)
                     /locus_tag="OO160_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HCR5526215.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQ"
BASE COUNT          132 a           64 c           61 g           94 t
ORIGIN      
        1 atttgtttcc ggaatgtctc gggagaaagt gtaaccagtc ctgggcaatg gctgacatga
       61 tgatacatta ataccggaac atgatttgct ttaaacaaaa tgccccctcc gtttaaaatt
      121 taaagtttct ggcacccagt aagtagagat aaaaggaaag cctaataaag gtggtgaact
      181 ttatatacta ctaaatcaaa catcaacaat ttataaacaa taatatatta tcatatcttt
      241 taaaaacggc tcactaagaa ccttgaggtg gcacactaaa tttagctacc tgaacagagg
      301 tgatatgctc acctcagaac aacacaggtg ccataatgaa aaaagaaatt t
//
LOCUS       DAJTNP010000366          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTNP010000366 DAJTNP010000000
VERSION     DAJTNP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            223..>347
                     /locus_tag="OO160_004388"
     CDS             223..>347
                     /locus_tag="OO160_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5526216.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIV"
BASE COUNT          116 a           68 c           75 g           88 t
ORIGIN      
        1 caacattttt aaaaatcgac gaagatcgtc caggagcgcc gtttttcaag ggttggatag
       61 atgattcgtt taccatgtcg acgaacttcg acgaatttcg ccgataaaaa gtgtcccata
      121 catgccccat tgatgcccca taaacaatat caggaattga cactggttat ccatacagat
      181 aaaaataaca cctgtacata actacagtta caggagggtg ttatgcgtgt tgagatctgt
      241 atcgctaaag aaaaaatcac taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa
      301 ttaacccgac gcatcagtaa acgttatgac gatgtagagg tgatcgt
//
LOCUS       DAJTNP010000367          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTNP010000367 DAJTNP010000000
VERSION     DAJTNP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(88..>345)
                     /locus_tag="OO160_004389"
     CDS             complement(88..>345)
                     /locus_tag="OO160_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5526217.1"
                     /translation="VTVSSAGVAGVGGGATFAALIVLPAMGLPVTLVALLISVEPLID
                     MGRTALNVSGSMTAGTLTSQWLKQTDKAILDSEDDAELAHR"
BASE COUNT           89 a           98 c           90 g           68 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaac
//
LOCUS       DAJTNP010000368          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTNP010000368 DAJTNP010000000
VERSION     DAJTNP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>344
                     /locus_tag="OO160_004390"
     CDS             <1..>344
                     /locus_tag="OO160_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5526218.1"
                     /translation="IQGKLRMWGRWSYIGGGKTGNMFNQLLASKKLTKTAVNEALRRM
                     KKAGIEKPELEAFLREMINGKQKTWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQR
                     YEGRGMTKRKMA"
BASE COUNT          101 a           59 c          111 g           73 t
ORIGIN      
        1 attcagggaa aactgcgcat gtgggggcgc tggtcgtata ttggcggcgg taagacggga
       61 aatatgttta accagttgct ggcatccaaa aaattgacga aaacagccgt caatgaagcc
      121 ctgcgcagga tgaaaaaagc gggaatagag aaacctgagc tggaagcgtt tttgcgagag
      181 atgatcaatg gcaagcaaaa gacctggctg gcgcattgta ctgatgcaga ggcgttatgt
      241 attgatagag tcataagtga ggtgctggca gagcatcctg gattgattag tgtcctccgg
      301 caacggtatg aagggcgggg gatgactaag cgtaaaatgg ctga
//
LOCUS       DAJTNP010000369          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTNP010000369 DAJTNP010000000
VERSION     DAJTNP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO160_004391"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO160_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5526219.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTNP010000370          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTNP010000370 DAJTNP010000000
VERSION     DAJTNP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>340)
                     /locus_tag="OO160_004392"
     CDS             complement(<1..>340)
                     /locus_tag="OO160_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5526220.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJTNP010000371          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTNP010000371 DAJTNP010000000
VERSION     DAJTNP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>337
                     /locus_tag="OO160_004393"
     CDS             <1..>337
                     /locus_tag="OO160_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5526221.1"
                     /translation="MQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAPLF
                     EHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCA
                     VAAQVYIGSE"
BASE COUNT           75 a           75 c           99 g           88 t
ORIGIN      
        1 atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt gatgctggcc
       61 tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga tataaatatc
      121 gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta
      181 gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa ctctattctg
      241 gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt
      301 tgcgcggtgg cggcgcaggt ttatatcggc agcgaat
//
LOCUS       DAJTNP010000372          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTNP010000372 DAJTNP010000000
VERSION     DAJTNP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..326)
                     /locus_tag="OO160_004394"
     CDS             complement(<1..326)
                     /locus_tag="OO160_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5526222.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQL"
BASE COUNT           98 a           88 c           62 g           84 t
ORIGIN      
        1 agctgctcaa cgttcagttt cgctttggca taacaggcaa aatcataaaa atttgtcgcc
       61 tgctggctca acaaaaacac ggcattccca tgtttattaa tcccaacacc gttacgaatt
      121 ttacgtgagg cgacgttggg atgaatacgc ggattaatta caccgttttc catcaacatt
      181 ggtcctgact gcaccgcaaa ctgaatatct ttactggctt taaaagcatc cagacgaacg
      241 atgccgactt tatctcccgc gacataaaac acgccgccag gacggataaa gaaattccct
      301 tcacctgaag cgagatttaa cgccaccttc tg
//
LOCUS       DAJTNP010000373          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTNP010000373 DAJTNP010000000
VERSION     DAJTNP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(122..331)
                     /locus_tag="OO160_004395"
     CDS             complement(122..331)
                     /locus_tag="OO160_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5526223.1"
                     /translation="MFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
BASE COUNT          102 a           79 c           62 g           88 t
ORIGIN      
        1 attttcctga aacaaggtga atattcaaaa actcctgtca aattgccttt cgccctgaaa
       61 aatgcatagt caaatatctg ttttaactaa ttggcgttgc agtacatgca acgccaatta
      121 gttagcggaa tttacgtcga tactcgcctg gcgtcatccc aaagcgttgc ttaaataccg
      181 ttgaaaaatg actctggtca gaaaatcccc aatgaaagcc gatgcctgcc agtttttcat
      241 catctgcggc atggcgaatc gcatctgcac aaaaatcgag acgacggtta cgaatatatt
      301 gcgcgactac caaaccttta tcggcaaaca t
//
LOCUS       DAJTNP010000374          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTNP010000374 DAJTNP010000000
VERSION     DAJTNP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..>326)
                     /locus_tag="OO160_004396"
     CDS             complement(<1..>326)
                     /locus_tag="OO160_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526224.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNA"
BASE COUNT           59 a          115 c           72 g           80 t
ORIGIN      
        1 gcgcgttttt cttcagcccc tccacattca gtcccgcgat ggcccggcgg tataccatcc
       61 ggccgccaca cagcacgcat tcgaacgggt cacgactcag gaactgtttc accatttgtg
      121 catagcacac tttcgccact ggttccggtt tatccatccc cagagcacgg tacacctgcg
      181 gcagcttctc tccacacacc cggttggcaa ggaacccgaa gtacctcacc atcttaaaaa
      241 acttctccgg gatgtgctgt ttcagcctcg cgaccagctc acgctgtgtc agcgtttccg
      301 tcgccgtttc tcccgttttg tggtcc
//
LOCUS       DAJTNP010000375          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTNP010000375 DAJTNP010000000
VERSION     DAJTNP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>321
                     /locus_tag="OO160_004397"
     CDS             <1..>321
                     /locus_tag="OO160_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR5526225.1"
                     /translation="VKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTC
                     TCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLW
                     DALA"
BASE COUNT           84 a           66 c           88 g           83 t
ORIGIN      
        1 acgtcaagaa aggggttgca aggtttacca tcgattcttc gttatatgtc gcacatgcgg
       61 atgacattgt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac gttaacgatc
      121 cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct gaggaaaatc
      181 agttactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa gaagtcataa
      241 aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc caaacatctt
      301 cgttatggga tgcattagcc t
//
LOCUS       DAJTNP010000376          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTNP010000376 DAJTNP010000000
VERSION     DAJTNP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..62)
                     /locus_tag="OO160_004398"
     CDS             complement(<1..62)
                     /locus_tag="OO160_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5526226.1"
                     /translation="MKRSIIAAAVFSSFFMSAGV"
BASE COUNT          134 a           40 c           38 g          104 t
ORIGIN      
        1 aatactccag cgctcataaa aaaagaagaa aagacagcgg cagcaataat tgaacgtttc
       61 ataatatgtt ccttaataaa atttattttt ggttgttaag agtctgtatc tcttggaaac
      121 aaatttctca catcaaaaaa gctgccgcaa tgtaaacata ttgttttatt gatttaaaac
      181 aaattaacca atgcaatata aatacaacaa atacttaaga atattaaata tttatcacat
      241 ctattaatta atgtagattt atgtaaatgg tattgttgat atcaataaaa aagacgaaat
      301 catttatata aatgat
//
LOCUS       DAJTNP010000377          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTNP010000377 DAJTNP010000000
VERSION     DAJTNP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..>312
                     /locus_tag="OO160_004399"
     CDS             <1..>312
                     /locus_tag="OO160_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5526227.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     ND"
BASE COUNT           91 a           52 c           98 g           71 t
ORIGIN      
        1 ggcggtaaga cggggaatat gtttaacctg atgttgacct ctaaaaagct gacaaaaacg
       61 gcaattaacg aggcgctccg gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag
      121 gcttttttgc gggatatgat taacggtaag caaaagagct ggctggcgca ttgtactgat
      181 gcagaggcgt tatgtattga tcgggtcata agtgaggtgc tggcagagca tccaggattg
      241 attagcgtcc ttcggcaacg gtatgagggg cgggggatga ccaaatgcaa aatggctgaa
      301 ctgctgaatg at
//
LOCUS       DAJTNP010000378          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTNP010000378 DAJTNP010000000
VERSION     DAJTNP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..269
                     /locus_tag="OO160_004400"
     CDS             <1..269
                     /locus_tag="OO160_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5526228.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
BASE COUNT           79 a           84 c           87 g           60 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc
//
LOCUS       DAJTNP010000379          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTNP010000379 DAJTNP010000000
VERSION     DAJTNP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..233
                     /locus_tag="OO160_004401"
     CDS             <1..233
                     /locus_tag="OO160_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5526229.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT           77 a           67 c           78 g           88 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 gtcgtcagag ctgcgatata tccccttcct ccatgggctg tctcttatac acaaatcccc
      301 acccggggat
//
LOCUS       DAJTNP010000380          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTNP010000380 DAJTNP010000000
VERSION     DAJTNP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            complement(<1..169)
                     /locus_tag="OO160_004402"
     CDS             complement(<1..169)
                     /locus_tag="OO160_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526230.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSP"
BASE COUNT           83 a           81 c           86 g           59 t
ORIGIN      
        1 tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg tacatttgga
       61 aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg ttggcgtaac
      121 cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata aatcgcgaat
      181 gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct ccgtaaacat
      241 ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag ccccggataa
      301 cagattgag
//
LOCUS       DAJTNP010000381          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTNP010000381 DAJTNP010000000
VERSION     DAJTNP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            23..>301
                     /locus_tag="OO160_004403"
     CDS             23..>301
                     /locus_tag="OO160_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5526231.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVD"
BASE COUNT           87 a           64 c           77 g           73 t
ORIGIN      
        1 gtaaggctgc gagaggtgaa taatgtcgcc atcattacgc aaggctgttg cagttgctat
       61 tggtggcggg gctgttgcta tagcatctgt gttaatcact ggcccaggtg gtaacgatgg
      121 tctggaaggt gtcagctaca taccatacaa agatatcatt ggtgtatgga ctgtatgtca
      181 cggacacacc ggaaaagaca tcatgcctgg taaaacgtat accgaagcag aatgcaaagc
      241 tctcctgaat aaagaccttg tcactgtcgc cagacaaatt aacccgtaca tcaaagtcga
      301 t
//
LOCUS       DAJTNP010000382         8782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.guided.142, whole genome shotgun sequence.
ACCESSION   DAJTNP010000382 DAJTNP010000000
VERSION     DAJTNP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.guided.142"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            263..922
                     /locus_tag="OO160_004404"
     CDS             263..922
                     /locus_tag="OO160_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5526232.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO160_004405"
     CDS             complement(1123..1500)
                     /locus_tag="OO160_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5526233.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="OO160_004406"
     CDS             complement(1567..4533)
                     /locus_tag="OO160_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5526234.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="OO160_004407"
     CDS             complement(4536..5096)
                     /locus_tag="OO160_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5526235.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="OO160_004408"
     CDS             complement(5222..5572)
                     /locus_tag="OO160_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5526236.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="OO160_004409"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="OO160_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5526237.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="OO160_004410"
     CDS             6998..7828
                     /locus_tag="OO160_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5526238.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8732
                     /gene="aadA1"
                     /locus_tag="OO160_004411"
     CDS             7941..8732
                     /gene="aadA1"
                     /locus_tag="OO160_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5526239.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1996 a         2356 c         2479 g         1951 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     8641 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     8701 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     8761 gcttcgcggc gcggcttaac tc
//
LOCUS       DAJTNP010000383         6563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.guided.162, whole genome shotgun sequence.
ACCESSION   DAJTNP010000383 DAJTNP010000000
VERSION     DAJTNP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.guided.162"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO160_004412"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO160_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5526240.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="OO160_004413"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="OO160_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /locus_tag="OO160_004414"
     CDS             2144..2617
                     /locus_tag="OO160_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5526241.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="OO160_004415"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="OO160_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="OO160_004416"
     CDS             2967..3803
                     /locus_tag="OO160_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5526242.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="OO160_004417"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="OO160_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="OO160_004418"
     CDS             complement(4358..4747)
                     /locus_tag="OO160_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526243.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="OO160_004419"
     CDS             4965..5405
                     /locus_tag="OO160_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5526244.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6183..6332)
                     /locus_tag="OO160_004420"
     CDS             complement(6183..6332)
                     /locus_tag="OO160_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526245.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1622 a         1700 c         1696 g         1545 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggataaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgtata
     5821 agtgatgtat aagtgatgta aaagtgatgt atgaactgaa ggagcgatgt aattctgtag
     5881 tcaggaagaa taacccgttc atgcaggcat gaacgataca gtgaggaacg ctttttttgt
     5941 cacgatctcc ccttaacctg cgtaaatcac ccgccatggt tgattatttt tcaacctcaa
     6001 aatggtcata cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa
     6061 aaaaggaata tttcgttttc acgagtaata cccgggtatt aaaaatccac tgtaattaca
     6121 tgaaaataaa gaaataaaag gtaataccgg agtattacca cgtacgcggg caagcgaaaa
     6181 agctataacg cttcccggcc gacaggcgac ccaaaatcca caggggcatg caggttctct
     6241 tcagtaacct gcgccattaa atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc
     6301 gaatccagtt gttcatcata ttcagatggc atggggtgat atccagtagc tgagaggttg
     6361 ttagcgggtt tcggggcgca gccctgaacc agtcatgtag cgctagcgga gtgtatactg
     6421 gcttactatg ttgccacgat tgcgaaaatc agtgaagtgc ttcatgtggc aggagaaaaa
     6481 aggcagcacc ggcgcgtcag cagaatatgc gatacaggag atattccgct tcctcgctca
     6541 ctgactcgct acgctcggtc gtt
//
LOCUS       DAJTNP010000384         6562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.guided.163, whole genome shotgun sequence.
ACCESSION   DAJTNP010000384 DAJTNP010000000
VERSION     DAJTNP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.guided.163"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO160_004421"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO160_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5526246.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1640..2122
                     /locus_tag="OO160_004422"
                     /pseudo
     CDS             1640..2122
                     /locus_tag="OO160_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2143..2616
                     /locus_tag="OO160_004423"
     CDS             2143..2616
                     /locus_tag="OO160_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5526247.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2691..2966
                     /locus_tag="OO160_004424"
                     /pseudo
     CDS             2691..2966
                     /locus_tag="OO160_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2966..3802
                     /locus_tag="OO160_004425"
     CDS             2966..3802
                     /locus_tag="OO160_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5526248.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3909..4313)
                     /locus_tag="OO160_004426"
                     /pseudo
     CDS             complement(3909..4313)
                     /locus_tag="OO160_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4357..4746)
                     /locus_tag="OO160_004427"
     CDS             complement(4357..4746)
                     /locus_tag="OO160_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526249.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4964..5404
                     /locus_tag="OO160_004428"
     CDS             4964..5404
                     /locus_tag="OO160_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5526250.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6182..6331)
                     /locus_tag="OO160_004429"
     CDS             complement(6182..6331)
                     /locus_tag="OO160_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5526251.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1622 a         1700 c         1696 g         1544 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat atttttttgg
     1621 tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg
     1681 acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc
     1741 tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat
     1801 caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc
     1861 ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact
     1921 tggcgctgtt cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat
     1981 gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga
     2041 cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt
     2101 gcggctcgac caagagcgca ccgttaaccc aggataagaa ccttgaaagt atcattgata
     2161 gctgcgaaag cgaaaaacgg cgtgattggt tgcggtccag acataccctg gtccgcgaaa
     2221 ggggagcagc tactttttaa agcattgacc tacaatcagt ggcttctggt gggtcgcaag
     2281 acgtttgaat ctatgggcgc actccccaat aggaaatacg cggtcgttac ccgctcaggt
     2341 tggacatcaa atgatgacaa tgtagttgta tttcagtcaa tcgaagaggc catggacagg
     2401 ctagctgaat tcaccggtca cgttatagtg tctggtggcg gagaaattta ccgagaaaca
     2461 ttacccatgg cctctacgct ccacttatcg acgatcgaca tcgagccaga gggggatgtt
     2521 ttcttcccga gtattccaaa taccttcgaa gttgtttttg agcaacactt tacttcaaac
     2581 attaactatt gctatcaaat ttggaaaaag ggttaacaaa gctatgcaat cgacggcaaa
     2641 aagcttcgtt cgcttcgcgc actacgcctt tttccgcgat tgatagcgac gatatggttg
     2701 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
     2761 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
     2821 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
     2881 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
     2941 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
     3001 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
     3061 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     3121 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     3181 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     3241 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     3301 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     3361 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     3421 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     3481 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     3541 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     3601 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     3661 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     3721 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     3781 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     3841 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     3901 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     3961 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     4021 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     4081 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     4141 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     4201 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     4261 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     4321 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     4381 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccactcttac
     4441 attgttcgca gtatctaggt acatgttatt gcgtgcacct tcatgatgta taatgttatt
     4501 aatgacagca gaaagatcga cagaattaat attcttcgta cctgggacag ttatcgggtg
     4561 tcggccagtg atgagaccaa aggatgcagt ataagatgaa ccactactat tatcatggcc
     4621 accattacca ccaccgttaa aactacctcc cgaacttcca gagccgctat gaccatcatt
     4681 gtaggggtca tttggtccca tgtgaccaaa accaccgaca gtaccgctat ttaactctgt
     4741 actcataaaa ttatcctctt tgacttttaa aaacagcaaa aactgtattt ataacaatgt
     4801 gtttatatac agtattcgaa atactacgcc cgtcaaaacc acaccgtcaa caggtttttt
     4861 gtgtattttt cacgcctcac gggcagcctc agaccttttg tgttaaaaca gtaatatgtt
     4921 ctgctgttac aatacggtac acacaggaga cactgtcact tttatggcat ggcacacagt
     4981 tcaagagaca ctgaaattaa caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa
     5041 aggccttgtc agctatcgca caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact
     5101 gattcgggcc tatggcgagc tgcgcacttc tgagacacca aaaaggcaca aggagggaca
     5161 ctccaaatct gtcgttagcc aggcggaaaa tgctcagcta gacgtcattc agcagaaact
     5221 ggacgctctg cagcagactg ttaccctaat gcttgaagac aagatggcgc gggaagcaga
     5281 aaaccgcctg cgaaatgagg agagggaaca actacaggcc gaaattagcc gtctggctga
     5341 cgcgctggca caggaaaaaa agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga
     5401 gtaacagtgc aggtgactct gatttaccat aacatctgtt atcagcaccg cctgagaggc
     5461 cctcagggcg tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc
     5521 cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcgcacaag tgcatagcgc
     5581 ggaaattcac accattcagc cggggtaagt cctgtttccg gcgttcttcc catccgaaaa
     5641 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
     5701 cccgctgtca gcacgttcag gaagggggtg atgtacaaat ggcacagagg atgaaagtga
     5761 cgtatgagct gaaggaatga tgtaaaagtg atgtataagt gatgtaaaag tgatgtataa
     5821 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5881 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5941 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     6001 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     6061 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     6121 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     6181 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     6241 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     6301 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     6361 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     6421 cttactatgt tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     6481 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     6541 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJTNP010000385         3889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.guided.207, whole genome shotgun sequence.
ACCESSION   DAJTNP010000385 DAJTNP010000000
VERSION     DAJTNP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.guided.207"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            <1..301
                     /locus_tag="OO160_004430"
     CDS             <1..301
                     /locus_tag="OO160_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR5526252.1"
                     /translation="LYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVN
                     FLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            294..1028
                     /gene="frdB"
                     /locus_tag="OO160_004431"
     CDS             294..1028
                     /gene="frdB"
                     /locus_tag="OO160_004431"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5526253.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1039..1434
                     /gene="frdC"
                     /locus_tag="OO160_004432"
     CDS             1039..1434
                     /gene="frdC"
                     /locus_tag="OO160_004432"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5526254.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1445..1804
                     /gene="frdD"
                     /locus_tag="OO160_004433"
     CDS             1445..1804
                     /gene="frdD"
                     /locus_tag="OO160_004433"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5526255.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1867..3000
                     /locus_tag="OO160_004434"
     CDS             1867..3000
                     /locus_tag="OO160_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5526256.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3089..3622
                     /gene="blc"
                     /locus_tag="OO160_004435"
     CDS             3089..3622
                     /gene="blc"
                     /locus_tag="OO160_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5526257.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3619..>3889)
                     /gene="sugE"
                     /locus_tag="OO160_004436"
     CDS             complement(3619..>3889)
                     /gene="sugE"
                     /locus_tag="OO160_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5526258.1"
                     /translation="AVGLKYTHGFSRLTPSVITVTAMIVSMALLAWAMKSLPVGTAYA
                     VWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT          949 a         1024 c         1017 g          899 t
ORIGIN      
        1 gctctacacc attgaactga gccacggtct gaacgttgct gaatgtatgg cgcactccgc
       61 aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg
      121 tgacgacgtc aactttctca aacacaccct cgccttccgc gatgctgatg gcacgactcg
      181 cctggagtac agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg
      241 cgaagcggat gcagccgata aggcggaagc agccaataat aaggagaagg cgaatggctg
      301 agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc
      361 atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct
      421 acatcaaaga caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt
      481 gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc
      541 gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc
      601 tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg
      661 gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt
      721 atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg
      781 gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag
      841 atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggtgtat
      901 ggagctgtac tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg
      961 cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac
     1021 cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc
     1081 acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt
     1141 ccggctgtgt ggttcagcat tgaactgatt ttcgcgctgt ttgccctgaa aaatggcccg
     1201 gaagcctggg cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg
     1261 atcactctga cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg
     1321 gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg
     1381 gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc
     1441 tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg
     1501 ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct
     1561 gctgccactg gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc
     1621 gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg
     1681 tttacaccgt atgcaccacg cgatgcacga tctgaaaatc cacgtgcctg cgggcaaatg
     1741 ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggtg tcgttacaat
     1801 ctaacgcatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca
     1861 gaccttatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctctta ctccacattt
     1921 gctgcccctc aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa
     1981 caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc
     2041 tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta
     2101 ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc tcgaggggaa
     2161 atcaagttaa gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaat
     2221 gggatcacac tattacatct tgcgacctac accgctggcg gcctgccatt gcaggtgccg
     2281 gatgaagtga aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg
     2341 gctccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct
     2401 gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc
     2461 aaactcaacc atacgtggat taatgtacct cccccagaag aaaagaatta cgcctgggga
     2521 tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt tagatgctga agcttatggt
     2581 gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa gcaatttacc cccccttgat
     2641 atcaatgaga aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc
     2701 ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc
     2761 atcattaacg gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag
     2821 cccccaactc ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt
     2881 ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa
     2941 aactatccca atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa
     3001 aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac
     3061 ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac
     3121 agctgcattt ctggtcgttg cctgtagttt tcctacgccg ccgcgtggcg tgaccgtagt
     3181 gaataatttc gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca
     3241 ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg
     3301 cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg
     3361 gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc
     3421 tttctatggc ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg
     3481 cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt
     3541 gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg
     3601 ggtacagcag cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat
     3661 taacgccaga ctcgccagac gcatcgggtt agcggactca ccgagcagca caatgccggt
     3721 gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc ctaccggtaa
     3781 cgatttcatc gcccaggcaa gtagcgccat actgactatc atcgccgtca cggtaataac
     3841 actcggcgtc aaacggctaa agccgtgggt atatttcagg ccaacggcc
//
LOCUS       DAJTNP010000386         3525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.guided.213, whole genome shotgun sequence.
ACCESSION   DAJTNP010000386 DAJTNP010000000
VERSION     DAJTNP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.guided.213"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            68..283
                     /locus_tag="OO160_004437"
     CDS             68..283
                     /locus_tag="OO160_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5526259.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            276..662
                     /locus_tag="OO160_004438"
     CDS             276..662
                     /locus_tag="OO160_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5526260.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            670..1356
                     /locus_tag="OO160_004439"
     CDS             670..1356
                     /locus_tag="OO160_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5526261.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1334..1957)
                     /gene="tetR(B)"
                     /locus_tag="OO160_004440"
     CDS             complement(1334..1957)
                     /gene="tetR(B)"
                     /locus_tag="OO160_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5526262.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2039..3244
                     /gene="tet(B)"
                     /locus_tag="OO160_004441"
     CDS             2039..3244
                     /gene="tet(B)"
                     /locus_tag="OO160_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5526263.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3357..>3525)
                     /locus_tag="OO160_004442"
     CDS             complement(3357..>3525)
                     /locus_tag="OO160_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5526264.1"
                     /translation="QENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAI
                     YRGFLLSLKDK"
BASE COUNT          972 a          650 c          771 g         1132 t
ORIGIN      
        1 tgaacataaa acactatcaa taagttggag tcattaccga ccactattaa gtcatgtagc
       61 tggttttatt tcaattgaac gttttcaaag tctagctaca gaaggaaaaa tgttatcgct
      121 atcttggtgg gaaaacgaat acgcagttct gcaatggaaa aatcatgttt tacatgcgaa
      181 agctcaacaa gaagggcgag agtcaatatt tgatttttac aaaattagta ttgctcatat
      241 tactcgcgaa tattcattta aaaaggacaa ggataatgtt tgatgttcac gttgttttag
      301 ataatcaaat aggacaatta gcattactag gaaaaacatt aggtaataaa ggtattggat
      361 tggaaggggg agggatattt acggttggtg atgaatgcca tgctcatttt cttgttgaac
      421 aaggaaagga agctaaaata gcgctagagc aagctggact gttagtactt gcgatccgga
      481 caccattaat tcgtaagtta aaacaggaaa aaccggggga acttggcgaa atagcacgag
      541 tattggcgga gaataacatt aatattttag tgcaatacag tgaccatgct aaccaactga
      601 tattaataac ggacaatgat agtatggctg catctgttac gctcccttgg gcaataaagt
      661 gaacttgcga tggctaattt aatacgaaaa gaggttacct ttgagtcctc aatagccgcg
      721 ataggggcgg ctatgtctga catttcacga gttaaaatac tcagtgcttt gatggatggg
      781 cgagcttgga cggccactga gctaagttct gtggcgaata tatcagcttc aacggcgagc
      841 agtcatttat ctaaattatt agattgccag ctaatcacag tagtagctca aggcaagcat
      901 cgttattttc ggctagcagg aaaagatatt gctgaattga tggaaagtat gatggggatc
      961 tccttaaacc atggcgtaca tgccaaagtt tccacgccag tgcatttacg aaaagcacgt
     1021 acttgctatg atcatttagc tggcgaagtt gccgttaaga tctatgattc cctttgtcaa
     1081 cagcaatgga tcactgaaaa tggttcaatg atcacattaa gtggtattca atattttcat
     1141 gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa tctgttgtgc gtgtttagat
     1201 tggagtgaac gccgtttcca tttaggtggg tacgttggag ccgcattatt ttcgctttat
     1261 gaatctaaag ggtggttaac tcgacatctt ggttaccgtg aagttaccat cacggaaaaa
     1321 ggttatgctg cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga
     1381 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
     1441 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
     1501 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg
     1561 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
     1621 gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat
     1681 gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt
     1741 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
     1801 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
     1861 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
     1921 cgctgttaat cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga
     1981 cactctatca ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat
     2041 gaatagttcg acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct
     2101 tatcatgcca gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa
     2161 ccactttggc gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct
     2221 tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc
     2281 atcgctggat tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg
     2341 tttgctttca gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac
     2401 cacctcagct tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg
     2461 tttaatagcg gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt
     2521 ttttatcgct gcgttgctaa atattgtcgc tttccttgtg gttatgtttt ggttccgtga
     2581 aaccaaaaat acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc
     2641 ggtatacatc actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca
     2701 attgataggc caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg
     2761 gaatagcatg atggttggct tttcattagc gggtcttggt cttttacact cagtattcca
     2821 agcctttgtg gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg
     2881 atttattgca gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt
     2941 tttccctgtt ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt
     3001 gatgtctatc caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct
     3061 taccaatgca accggtgtta ttggcccatt actgtttgct gttatttata atcattcact
     3121 accaatttgg gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct
     3181 gctatcgatg accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc
     3241 ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataacc
     3301 caagagggca ttttttacga taaagaagat ttagcttcaa ataaaaccta tctattttat
     3361 ttatctttca agctcaataa aaagccgcgg taaatagcaa taaattggcc ttttttatcg
     3421 gcaagctctt ttaggttttt cgcatgtatt gcgatatgca taaaccagcc attgagtaag
     3481 tttttaagca catcatcatc ataagcttta agttggttct cttgg
//
LOCUS       DAJTNP010000387         3512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103437
            SAMEA8899885-rid15821763.guided.214, whole genome shotgun sequence.
ACCESSION   DAJTNP010000387 DAJTNP010000000
VERSION     DAJTNP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899885
            Sequence Read Archive: ERR6005291
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478205.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478205.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:18:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 395
            CDSs (without protein)            :: 395
            Pseudo Genes (ambiguous residues) :: 0 of 395
            Pseudo Genes (frameshifted)       :: 183 of 395
            Pseudo Genes (incomplete)         :: 110 of 395
            Pseudo Genes (internal stop)      :: 145 of 395
            Pseudo Genes (multiple problems)  :: 42 of 395
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899885-rid15821763.guided.214"
                     /serovar="S. flexneri 2a"
                     /isolate="103437"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-06-25"
     gene            13..471
                     /gene="gpt"
                     /locus_tag="OO160_004443"
     CDS             13..471
                     /gene="gpt"
                     /locus_tag="OO160_004443"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5526265.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            563..1807
                     /gene="frsA"
                     /locus_tag="OO160_004444"
     CDS             563..1807
                     /gene="frsA"
                     /locus_tag="OO160_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5526266.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            1865..2266
                     /gene="crl"
                     /locus_tag="OO160_004445"
     CDS             1865..2266
                     /gene="crl"
                     /locus_tag="OO160_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5526267.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(2305..3360)
                     /gene="phoE"
                     /locus_tag="OO160_004446"
     CDS             complement(2305..3360)
                     /gene="phoE"
                     /locus_tag="OO160_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5526268.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT          862 a          850 c          868 g          932 t
ORIGIN      
        1 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
       61 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
      121 cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
      181 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
      241 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
      301 gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
      361 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa
      421 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
      481 cgcctggcat tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt
      541 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
      601 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
      661 gcctgtgcag tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg
      721 tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat
      781 tgtgatgagc gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg
      841 tggtggcaac tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg
      901 tacggaagaa gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa
      961 cattgccgcc tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc
     1021 aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt
     1081 taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc
     1141 gttcccgaca gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct
     1201 gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt
     1261 gggcttttct tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt
     1321 aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg
     1381 tttcggcgct aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt
     1441 tgcctgtctt ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt
     1501 gccggaaatg tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga
     1561 agcgttacgc gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg
     1621 ctgcccaacg ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga
     1681 ctcacgctta atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc
     1741 ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt
     1801 gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat
     1861 agcaatgacg ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact
     1921 aggcccgtat attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc
     1981 tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga
     2041 gctggaagcg caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc
     2101 aggcgactgg aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac
     2161 cctgcgtgag tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga
     2221 accggcgaat gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg
     2281 atgcatcaca tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc
     2341 atcattatta atattcagct tattatcgct atccagttgg ttgattttat aatcaacaaa
     2401 cgctgacata tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc
     2461 ttcatcaccg atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg
     2521 cagaccaaag tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa
     2581 gccgccagtt attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt
     2641 attggcatcg tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct
     2701 ttgcaggttc tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc
     2761 gctgccgcca aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac
     2821 gtcgcggttt tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc
     2881 gaagaagtcg gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc
     2941 ggtctgcgcc gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc
     3001 atacaacgcg cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc
     3061 ggcaaaagcg agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa
     3121 ctccgcttcc caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa
     3181 accaaaacgg atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat
     3241 ggctttaact ttgccataga catccagttt attaccgtct ttattatata tttctgcagc
     3301 ctgtacagat gcagatgcca caatgcccat caccactaat gccagagtgc tctttttcat
     3361 tttcattcct gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag
     3421 gaaagttttt aacctgagat tgttaaagat atattacaga ttaatgatat tctgaaaatg
     3481 ggtaatgctg ccaacttact gatttagtgt at
//
